LOCUS       DAOOLB010000001        77672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_173_length_77672_cov_46.340686, whole genome shotgun sequence.
ACCESSION   DAOOLB010000001 DAOOLB010000000
VERSION     DAOOLB010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 77672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 77672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77672
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_173_length_77672_cov_46.340686"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1171
                     /locus_tag="PLF77_00005"
     CDS             41..1171
                     /locus_tag="PLF77_00005"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC07262.1"
                     /translation="MYIAQKQLEKLRNLAVPGKVVVIYGARRTGKTTLLKEFLKDETK
                     DHLLVNGEDITVHSYLSSQSVEKLTSFVGNAKLLIVDEAQKIPNIGINLKLIVDHLSG
                     IRMVVTGSSSFDLARHTGEPLTGRKYTLKLFPLAQMEIGQIEKIHQTQAHLENRLIYG
                     SYPEVVLMQDNQGREKYLKEIVSSYLYKDILELDGIRQSAKIIRLLQLLAFQIGKEVS
                     LTELGTSLSMSKNTVERYLDLLEKAFVLHRLTGFSRNLRSEIIKNCRYYFLDNGVRNA
                     LINNFNPLSQRNDCGELWENYLIMERLKRQEYLDTPANNYFWRTYTRQEIDFVEERQG
                     RLFGYEMKWGKAKPKPPKLWQSAYPESSYELINRENYLKFIT"
     gene            1343..1936
                     /locus_tag="PLF77_00010"
     CDS             1343..1936
                     /locus_tag="PLF77_00010"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HPC07263.1"
                     /translation="MATVKRIKSDNVFQLKITLNHIKPPIWRRVLVDSDIKLPDLHKI
                     IQTVMGWTNSHLHQFIIGNQYYSLPSDESFYKVVDYRRIKLDSLFNTPKSNFIYEYDF
                     GDGWEHSIVIEKILPRKKNTYYPICIDGKRSCPPEDCGGTFGYENLITIINNPEHEEY
                     DEMMEWLGDYFDPKEFNIDEVNEFLREKNFGCVELFD"
     gene            2074..3087
                     /locus_tag="PLF77_00015"
     CDS             2074..3087
                     /locus_tag="PLF77_00015"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPC07264.1"
                     /translation="MVGQAEKIKFTVDKSQGGQRLDIYLAQSEAALSRSQVKYAIEEG
                     DVLVNDKVPKVSQHLKEGDVIVLTKRPPVEAIAVPQEMPLDIVYEDDAVIVINKPAGM
                     VVHPAPGNPDKTLVNALLFHCHDLSGIGGVLRPGIVHRLDKDTSGLIVAAKSDDAHRN
                     LSSQFEKHDVHKKYLALVWGDVKGNSGEIVLPVGRHTNNRKKMSTKSRHGKGALTLWK
                     VRERFGVATLLDVEIKTGRTHQIRVHLSDRGYPVVGDAVYGNSAKINTVKDPLLKAKI
                     KEFNRQALHAAQLSFIHPQKGERVVFTADMPQDLADLCAQLRTSIKSVDSKKDMWKDK
                     LKG"
     gene            3091..3945
                     /gene="pgeF"
                     /locus_tag="PLF77_00020"
     CDS             3091..3945
                     /gene="pgeF"
                     /locus_tag="PLF77_00020"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HPC07265.1"
                     /translation="MFQLNKKHSIQYYESSFLSGCDFLTHAFCTRLGGLSEDDYKSLN
                     ISFKEGDLESRVLQNWNRLAMAFAIPLENFLTLNQVHGDDIFIIKPYGDYLPSDGALN
                     YDAIVTPRENLAICIKTADCVPVFIVDRAKKIIAAVHAGWKGTALEISAKVVRLFLDK
                     YGSYPHDILAAVGPSIGLCCFEVDEDAADSFRNQKDHEAFLFAGNRPNKWIVDLPEAN
                     RRQMINCGISAANIDLSHLCTSCRQDLFYSHRGSGGITGRQLNFIMVKGEAPCRVMTI
                     GDEYPKIQ"
     gene            4147..>5070
                     /gene="rfaD"
                     /locus_tag="PLF77_00025"
     CDS             4147..>5070
                     /gene="rfaD"
                     /locus_tag="PLF77_00025"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HPC07266.1"
                     /translation="MIIVTGGAGFIGSAFVWKLNQEGIDNIIIVDRLGTSDKWKNLVN
                     LRFVDYIHKDDFLEMVDTDMMPFDVDAIIHMGACSSTTERDADYLWHNNYAYTKILAE
                     WALEHDIRFIYASSAATYGDGAKGFADSHKTIPGLKPINMYGYSKQVFDLWVLRQKLE
                     SRVAGIKFFNVFGPNEYHKGDMSSVIFKAFHQIKETGKVKLFKSYKKEYKDGGQMRDF
                     VYVKDCVNVMWWLLQNRKVNGIFNLGTGKARSWNDLIKAVFTAMKKKTNIEYIAMPEA
                     LRNQYQYFTEAEMSKLKKAGCPFKFSSLEDSV"
     assembly_gap    5071..5170
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5409..6155
                     /gene="kdsB"
                     /locus_tag="PLF77_00030"
     CDS             5409..6155
                     /gene="kdsB"
                     /locus_tag="PLF77_00030"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPC07267.1"
                     /translation="MKREVVCIIPSRYESSRFPGKPLADLCGKPMIQHVYERVAKAKA
                     VPYVAVATDDQRIFDAVKHFGGNVVMTAGTHRSGTDRIAEAVKSLNLPADAIVVNIQG
                     DQPIFEPTQVDEVIQPLLDDPSINMSTLIYKIVLDKEITHPHAVKVVLDHQNFALYFS
                     RATIPYVRDKNLRADYYKHHGIYAYRRDFLDVFTKLPEGKLEKLEALEQLRALEYGYK
                     IKVVVTPYDSVEVDNQQELERVREILLEKK"
     gene            6152..7324
                     /locus_tag="PLF77_00035"
     CDS             6152..7324
                     /locus_tag="PLF77_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HPC07268.1"
                     /translation="MNLKIIKKTCLLLWMFLLFTVAAAMPAGAADMENALMECAKIRN
                     NATRLKCFDKLARKPSIKEESTATTIEKKESVAVTAGDMETSEAAAKQTPLSVMEKYW
                     DMSLDPRKERNIFVLWPYRPCYFLPLAYNFSPNKDAALDFDPDAKAKHNEVKFQLSFK
                     FKLWRDIIRDHTIKNIIEKSTGIRGVDLWVAYTQQSFWQLYNDAFSAPFRDTNYEPEL
                     LLNFRMQREIPYLLGTKLQFVNIGFNHQSNGRSEPLSRSWNRIVANVGLEKNFGTATN
                     DSFELLLKTWYRLPEDDADDDNPDLSRYMGYGELWGTLYWKKQRFALMLRNNLRSDNK
                     GAVQLEWSMPMASINENLAKKISLYVQYFNGYGESLLDYNKNVNRISAGLMLADWY"
     gene            7366..7671
                     /locus_tag="PLF77_00040"
     CDS             7366..7671
                     /locus_tag="PLF77_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07269.1"
                     /translation="MKEIITATDDHTVESSLGWRVDILSMDALRYQERDKTITFEIED
                     YSDAIGELEWTIYIPPICKWQDENHPEEIIGQEKLDDIIDRISTAFWKLDMKIRGIA"
     gene            7886..8266
                     /locus_tag="PLF77_00045"
     CDS             7886..8266
                     /locus_tag="PLF77_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07270.1"
                     /translation="MSSDFSLEFKERYIHLIHAPDYEISPESNDILWAELAKACRKYK
                     CLKVLTEGSNQKRRITSMDAYQSGAQAAESIPGMTLAICIDDYTPDDITQFFKNVAYN
                     RGARIEFFNDKKKALAWLGFEDTE"
     gene            8328..8768
                     /locus_tag="PLF77_00050"
     CDS             8328..8768
                     /locus_tag="PLF77_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07271.1"
                     /translation="MTIHSYSKLEIATQQLDTALMLYFEDKNYFSVITLAGAAEEILA
                     VYLKLHKQPNAFEEMLDSSLLIGELLYNSKGSRDSMYKTINRVKNGSKHMKGRDDINI
                     QCNLKEEAQNILDRAVSNFYTLMTFENLNETSLMKKFNSQLVSK"
     gene            complement(9022..10683)
                     /gene="ilvD"
                     /locus_tag="PLF77_00055"
     CDS             complement(9022..10683)
                     /gene="ilvD"
                     /locus_tag="PLF77_00055"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPC07272.1"
                     /translation="MRSDLMKKGFERAPHRSLFKAMGYTDEELARPLIGVVNSANEII
                     PGHIHLDLITQDVKAGIRMAGGTPVEFPVIGVCDGIAMGHEGMKFSLASRELIADSIE
                     IMAMAHPFDALVFIPNCDKIIPGMLMAALRLNIPSIFISGGPMLAGKFQDKTVDLISV
                     FEGVGAVRAKKITAAELKQLEDCACPGCGSCSGMYTANSMNCVTEALGLGLPGNGTIP
                     AVLAARHRLAKRAGMKIMDLLKKNIRPRNIATLAAFKNAIAVDMALGCSTNTVLHIPA
                     VAHEAGIKLDLDLFNRISEKTPNLCKLSPAGRHHIEDLDAAGGIQAVMKVISALGVID
                     EKAITVTGKTVGGNLKDARVLNTDVIRTLKNAYSQQGGIAILRGNLAPDGAVVKQSAV
                     APAMQKNEGKARVFDCEDDAIKAILGGKIKGGDIVVIRYEGPKGGPGMREMLSPTSAI
                     VGMGLDKTVALLTDGRFSGGTQGAAIGHISPEAAEGGPIALVKEGDIIAIDIPAKKLN
                     LKVGAQELAKRRKALKPFKPRITTGYLARYAKMVTSSSTGAIFKD"
     gene            complement(10782..11840)
                     /gene="ilvC"
                     /locus_tag="PLF77_00060"
     CDS             complement(10782..11840)
                     /gene="ilvC"
                     /locus_tag="PLF77_00060"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HPC07273.1"
                     /translation="MAKINFGGVLEDVITAKEFPLKKAQKVLKNDVVAVLGYGVQGPA
                     QAANMKDNGINVIIGQAKEDTRYWKKAIADGWKPGKTLFPIEEAVKRGTIIQYLVSDA
                     AQKLLWPKVKANLKEGDALYFSHGFSIVYRKQTGVIPPKFVDVIMVAPKGSGTSVRRN
                     FLDGSGINSSFAVQQDYTGRAMERTLALGIAIGSGFLFPTTFQNEVYSDLTGERGVLM
                     GALAGIMEAQYNELRKHRHTPSEAFNETVEELTQSLIRLVAENGMDWMYANCSTTAQR
                     GALDWRHKFRKAVAPVFAELYKSVASGKETAIVLKANSAPDYRVKLEKELKQMRDSEM
                     WKAGAAVRELRPEKRKKK"
     gene            complement(11872..12357)
                     /gene="ilvN"
                     /locus_tag="PLF77_00065"
     CDS             complement(11872..12357)
                     /gene="ilvN"
                     /locus_tag="PLF77_00065"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HPC07274.1"
                     /translation="MEKKEHIISILVHNKPDVLARIAGILGGKGYNIESLCVNSTTHY
                     EVSKIVMTTVGTQDTINRIEGQLRRLVDVIQVDDLTNVESIHRELILVRLNLAADKKE
                     QIKKAVDMNKWKVIVNADTYVILEITGDRSQIDFALARLEPLGIADITRTGIVALNLE
                     N"
     gene            complement(12526..14229)
                     /gene="ilvB"
                     /locus_tag="PLF77_00070"
     CDS             complement(12526..14229)
                     /gene="ilvB"
                     /locus_tag="PLF77_00070"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HPC07275.1"
                     /translation="MKLKGTEILLKALEEEKVEIIFGYPGGAVLDIYDQIYKSNMKHI
                     LVRHEQGAVHAADGYARSTGKVGVCLVTSGPGATNTVTGIATANMDSIPLVVFTGQVP
                     TALIGNDAFQECDITGITRPCTKHNFLVRNIDDLAATIKEAFDIARSGRPGPVLVDLP
                     KNVMAAQTEYDPANIKIRNHEAAYKPAPKKMAGLLDLLTAAKKPLIMTGGGVILGRAS
                     SLLTEFARKYQMPVTGTLMGLGSFPGTDPLWLGMLGMHGTYYANMAISHCDLLIAVGV
                     RFDDRVTGTIDTFAAKAKIVQIDIDPSSINKNVAVDLPIIGDTQAVLKDLITFLDEHN
                     YAYDKDARNEWLNEIAGWKEKVPLTYCQNGNVIKPQSVIETLYKLTGGDAIITTEVGQ
                     NQMWTAQFFPFDKPNTFISSGGLGTMGFGFPAAIGVKCAFPDKHVVDIAGDGSIQMNI
                     QELATAAQYKINVKIVLLNNGYLGMVRQWQELFYEKRYSFTDMTYAPDFVKLAEAFGV
                     VGLRATKPEDVEPVLKEGLAIDKPVLMDFRVSREECVYPMVHPGDSISNMSLGSREMI
                     N"
     gene            complement(14811..16835)
                     /locus_tag="PLF77_00075"
     CDS             complement(14811..16835)
                     /locus_tag="PLF77_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HPC07276.1"
                     /translation="MFLNRKKSNRKNDGEAKKLSSLFNFFLGFVVFILVMTVFGVIGF
                     YAFTMDLPGIDALKDYRPSISSRVYDDKNELIDEFFLEDRKLIKIKEVPRVVVQAFVA
                     AEDSRFFAHKGFDLQSIFRAVFKNIEAGHIIQGGSTITQQVAKMMYLSPEKKYTRKFK
                     EAILAYKIDRYLTKDEILNLYLNQIYLGHGTYGIESASLGYFGKSARELKLHEAALLA
                     GMPKAPSNYSPFLHYDKAKQRQYYVLTRMKEDGYISQEEMEKAYAAPMNLRSIRPKDK
                     MAAYFVETVRRYVQEKYGADVLYKEGLSIYTTLDLTAQKYAGEAMERGLKELEERNKY
                     KSGLVQGALFCMDVRTGAIRAMVGGRDFNQSEFNRATQSRRQAGSAFKPIIYTAAFDK
                     GMTPATVIDDSPLAVDDVSQPDGIWRPKNFDDKFMGPITMRTALVLSRNVVTVKILQE
                     IGIDYAVSYAMNMGITSPLVRTLSLALGASGVTLQELVQAYGVLANQGQKVSPFFIKK
                     IVDRTGNVFEETKVQPEQVIDPRIAFITTHVMQDVVTSGTGTRVRSIGRPVAAKTGTT
                     NDTRDAWFIGMTPSLITGVWIGFDQEASLGSQEVGGRAAAPIWLYFMEKALQNTPVES
                     FPVPEGIVFEKINPKTGEPAGPSEKGTNEAFLLGTSPSSTLGDSIKGLFR"
     gene            complement(16844..17608)
                     /gene="tatC"
                     /locus_tag="PLF77_00080"
     CDS             complement(16844..17608)
                     /gene="tatC"
                     /locus_tag="PLF77_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPC07277.1"
                     /translation="MDNTQAAEDQKMSLTDHLLELRKRLTRSLIALAAGFGVCYYFKD
                     LIFDVMTRPLTQVLSKDSHLVYIGLTEAFFTYMKIAFFASLIITSPYILYQVWKFLSP
                     GLLPKEKQYVVPFVISSSLLFIGGVLFGYFIVLPTAFKFFVSFNNEYLQAMLSFKDYL
                     SLFVTFLLGFGLSFELPIFIFFLTKLGIVNAAKLSRQRRYAILVIFLVAAVLTPSPDA
                     LSQILMAIPLMILYEISIFVSKFAETKKEESQNDEG"
     gene            complement(17670..17930)
                     /gene="tatB"
                     /locus_tag="PLF77_00085"
     CDS             complement(17670..17930)
                     /gene="tatB"
                     /locus_tag="PLF77_00085"
                     /inference="COORDINATES: protein motif:HMM:NF011430.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HPC07278.1"
                     /translation="MFGIGMQELIIIAIIALLVVGPKKLPDLAKSLGKGFSEFRRAAD
                     GVTEDIKDAVKVDEKPKNDDEWKNSLLMKKPDHDNVKSDSSE"
     gene            18167..19225
                     /locus_tag="PLF77_00090"
     CDS             18167..19225
                     /locus_tag="PLF77_00090"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HPC07279.1"
                     /translation="MSRKTGVVKDGKYMQHSMGFAHPESPERLAVIYEMLENPLMSWK
                     FTPIEPREATHEEIERIHARSYVDFIASTAGKRSVFLDPDTSTSPETYDAAKLAAGGV
                     CNAIDSVMKGDVDNAFAFVRPPGHHAEKDTAAGFCVFNNIAIGAMHAIQKHNLKRILI
                     VDWDLHHGNGTQHSFYGDQRVLYFSTHQYPYYPGTGSISETGRGQGEYYTINVPLTMS
                     VGDAVFVKIFRKILEPVALEFKPELILLSAGFDTYYQDPLGAMQVTPEGYAAMTRILL
                     NIADTCCQGRFVAVLEGGYHLSGLVKSVKAVLNEMLDETHFSEERLSLMEKDVDERTT
                     ESVIDKVITRINPYWKVF"
     gene            19241..19528
                     /gene="gatC"
                     /locus_tag="PLF77_00095"
     CDS             19241..19528
                     /gene="gatC"
                     /locus_tag="PLF77_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HPC07280.1"
                     /translation="MKISSREVEYVAHLARLEITDAEKEKFTAQLNDILLYIDKLNEL
                     DTGDVKPISHAISITNAFREDKISNSIGTREALANAPDTRGEFFRVPKVID"
     gene            19590..21047
                     /gene="gatA"
                     /locus_tag="PLF77_00100"
     CDS             19590..21047
                     /gene="gatA"
                     /locus_tag="PLF77_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HPC07281.1"
                     /translation="MKLNQLTIHELQKRIKTGETSATQITHDVFARINAVDEKVHAYI
                     RLMQEEAFQAASKADEDIKQGDIRALTGIPIALKDIVCTKGIPTTCGSRILHNFIPPY
                     DATVVERLRKAGAIFVGKANMDEFAMGSSTETSYFGATRNPWDLERIPGGSSGGSAAA
                     VAADECIASIGSDTGGSIRQPAALCGTVGMKPTYGRVSRFGLIAFASSLDQIGPFTKD
                     VEDCAIMMNVLAGYDPKESTSVNEEVPDYRQFVGRDIKGWKIGIPKEYFIDGIDPGVH
                     AAIKKAITVIEQSGGQCVDISLPHTQYCVATYYIIAPAEASSNLARYDGVRYGLRAQD
                     VRDLLMMYKESRKQGFGAEVKRRIMIGTYALSAGYYDAYYKKASQVRALIKKDFDKAF
                     KICDVIMTPTSPTPAFKIGEKTDDPLQMYLSDIFTISANLAGIPGISVPCGFSSSGLP
                     IGVQFLASHFEEGKLIQIASAYEKNAKIEKRRPAL"
     gene            21047..22489
                     /gene="gatB"
                     /locus_tag="PLF77_00105"
     CDS             21047..22489
                     /gene="gatB"
                     /locus_tag="PLF77_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HPC07282.1"
                     /translation="MDFETVIGLEVHAQMLTDTKIFCNCSTKFGAAPNSHTCPVCLGM
                     PGVLPVLNKKVVEYALKMALATNCEINKSCEFARKNYFYPDLPKGYQISQYAYPLAEH
                     GYVMLETNGEQKKIGITRIHMEEDAGKLLHDEHNPVSYVDLNRTGVPLIEIVSEPDMR
                     SPEEAADYLKRLHEILVYLEICDGNMEEGSFRCDANVSIRPKGQKAFGTRTELKNMNS
                     FRNVQRALEYEIKRQQYLVENGGTVIQETRLWDDAQGATNSMRSKEEAHDYRYFPDPD
                     LVPILVDEAWVEKIRKELPELPLAKRERFIKDYQIPAYDAGVLTADKALALYYEKVVK
                     LCNKPKIASNWLMGDVMRFLNEDKRSVRECPIKAESLADMIRLIEEGAISGKMAKEVV
                     EDMYKTGKSPQNIIKEKGLVQITDRDALVKTASITVGKYPQQRDDYRGGGREKLFGFF
                     VGQVMKETKGKANPQLVNEILKEILDKKDD"
     gene            22482..23531
                     /gene="mtnA"
                     /locus_tag="PLF77_00110"
     CDS             22482..23531
                     /gene="mtnA"
                     /locus_tag="PLF77_00110"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPC07283.1"
                     /translation="MIKTIFWKNNAVVLIDQKALPLAEKYIVCKSYKAVIACIKDLTV
                     RGAPAIGVAAAMGAALGAIHLASLSPDKFRKKFYAICDEIAQARPTARNLFWALERMK
                     KIVSHSSQLSQRDIVKELIKKAKHICSEDIAINQQMGKNGSRIIADGDNILTHCNAGA
                     LATAGYGTALGVIRAARKQGKKLHVYVDETRPVLQGARLTAWELKKEKIPYTLITDNM
                     AGFLMQQKKIDKIIVGADRIAANGDTANKIGTYSLAVLAQAHGVPLYIAAPLSTIDVS
                     LKTGDSIPIEERKSDEVAHLQGTRSAPRGTIVYNPAFDITPARLITAMITEKGILRKP
                     FRASIARLYKGERGS"
     gene            23528..24142
                     /locus_tag="PLF77_00115"
     CDS             23528..24142
                     /locus_tag="PLF77_00115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPC07284.1"
                     /translation="MKLFLFDFDGVLVDSLDVYEKTVKDNLQKIKQPVVQSREEFLEL
                     FEDNFYTSLKNKGIDLNVFMKAAEDILAQVRITDMKPFPDVAPVIETLHKNHCLIVVS
                     SNDYASIKEALDLFHFNDYFRDILGSDFMFSKKEKILYAAEKYHVEPNKIYYIGDTTG
                     DIKEGKQAGVKTVGVTWGWHSRERMAAAEPDYLFDTPGELLQLS"
     gene            24153..24434
                     /locus_tag="PLF77_00120"
     CDS             24153..24434
                     /locus_tag="PLF77_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07285.1"
                     /translation="MNDLLAYISNWSVLKWVVLVLIAGFIGQFGRMAAEAIAKKIRAG
                     RPQQENKTIKPETPLQTPAPQPVSSGDLPDKKLLKAIAKAKKKEAKKKE"
     gene            complement(24436..25338)
                     /gene="ftcD"
                     /locus_tag="PLF77_00125"
     CDS             complement(24436..25338)
                     /gene="ftcD"
                     /locus_tag="PLF77_00125"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPC07286.1"
                     /translation="MKIIECVPNFSEGRDDKKILSIVSVFKMFPGVRLADFSGDADHN
                     RSVFTFLGKPDDVLDATLAACGKALELIDMRQQSGVHPRLGAVDVVPFIPLGRAKMKD
                     AVELAHAFGMRFSQRFGVPVYFYGYAARNGRNVELPDVRRGGYERLADKMSSTESLPD
                     AGENQFNARSGATAVGARDLLVAYNINLVSDDLLLARHIASRIREKGGGLKNVRAIGV
                     MLKRRRLAQVSVNLTDCKETPLKTVFDRVKALAAEGGAQILESELIGLAPKCAFAGAT
                     PEYLRLKDFDENRLLETHIRRLSE"
     gene            complement(25341..25985)
                     /locus_tag="PLF77_00130"
     CDS             complement(25341..25985)
                     /locus_tag="PLF77_00130"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPC07287.1"
                     /translation="MGKIYAIGDIHGCLTKLKTLISDIDIDFKKDTLVFIGDYVDRGE
                     FSRDVVDYVSGLQDSHENIVCLLGNHEQMFMNYLEGVDEEIYLRNGGMQTLRSYEIFL
                     SDDIKERKRKMSARHRKFFETLLPYYETEKYIFVHAGLRPGIPVAEQSQNDLLWIRYE
                     FINTEKDFDKTVVFGHTPLMTPLIDKNKIGIDTGAVYGGKLTCIEFPGLKIYQA"
     gene            complement(26013..26933)
                     /gene="ftsY"
                     /locus_tag="PLF77_00135"
     CDS             complement(26013..26933)
                     /gene="ftsY"
                     /locus_tag="PLF77_00135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPC07288.1"
                     /translation="MAKSSLFEKLKAGLVKTRTALTKSLDNLVFGKRVLDQELFEDLE
                     ELLISADMGPQFTYDLIDDVKQRVSRNELNNAQEIKKVLQERMTAILQKMEKPLIIPR
                     GKPFVIMTIGVNGSGKTTTIGKLANILKSEGHEILLVAADTFRAAAVEQLEVWGARVG
                     APVIKHKTNADPAAVVFDAMSKVKAGFNGVVIVDTAGRLHTRVNLMEELKKVKRIINK
                     ELPDVPPEILLILDATTGQNAVAQAKTFKEEIGATGIVLTKLDGTSKGGVVVRIASEL
                     ALPVWYIGVGEGVDDLRVFESSNFTKALLD"
     gene            complement(27000..30548)
                     /gene="smc"
                     /locus_tag="PLF77_00140"
     CDS             complement(27000..30548)
                     /gene="smc"
                     /locus_tag="PLF77_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HPC07289.1"
                     /translation="MNLKNLEISGFKSFRDRVVLDFSNGVTGIVGPNGCGKSNIVDAI
                     RWVMGEQRVKALRGKKMDDVVFNGSQDSAPVSMAEVTMTLYANGQSFPGAYSDLNEVS
                     ISRRVVLDGDSEYFINKVPCRLLDIKEFFMGTGVGARTYSLVEQGHVTNLVEAKPEDR
                     RLYIEDAAGVSKYKSRKEAAVRKMDLTKQNILRLNDIIKEVKTQLNTISRQAKKAEQY
                     KKIKQEIREAEITVALQTYVDFMARESDLQQQRTELQQQEALIRADFQAKESMLDEMK
                     VKLLENEELTAKVQQELYEIKNTISIKEKSIEFAQRQVTDMSGRREKDLTEIQVLQTR
                     KADLAAEIENLRKNSAEAENKIVALKAEHESLQQKVQELTEADKALSSQLEEKKILFI
                     DVVTEKAKLHNMISSLSKNIEDLKKREERETRELEEDQKMMADVCARLEAADEALKKE
                     KADIVGLEAKMAEAATELDRFRSDLQFNEEDIARLREDTSVKSTRLISLREFQESSKW
                     SNEGVKTIIENKEHSDKFYGVIADHISVPREYEAAVEAVLGEKLQYVVVQSQEDGVRA
                     IDYLKNYQLGRGSFISVDLRTHDARTYSEAHLNEAKPLLDKVTVRDDFKPIIDCLLGD
                     VLLIPTIENGISLWKKNGFRGTFVTPEGDIISPHGVLTGGSGSAVEKSLLATKREIGE
                     LEAEIAALSLDLETKVGRRTKILATISRWEEESSQMKTEIHALEITVNGRKKDRERFD
                     DDVNRLRQRIAVLEFNRQTLNTEKTEAEEKLRRFNEDVLQKDGEEKQINDIIAALNVQ
                     KEQSRVQIDEQERYVTAGKIELASLEEKKEGDVRTISRLQDHIDSMEQEQRTKEEDIV
                     FCEKQIAELTQGIENDQVLLKEIYSAFAAQEALLAEKKAEQNREDAQLKIREGEIREI
                     KKNLDELRQQANAMEFQSREITLNRENLRKGVAEKHDVDLAGMAADFEKVTEEKLVEI
                     VDALEKNKQMLETFGEVNLLALNEYEELDQRYNFLSTQIADLNASINVLQRTIARINK
                     ISRARFAETFEAVNACFKEVFAQIFPGGRGELLLTDENDMLETGVDIDIQVPGKRKQN
                     VSLLSGGEKSLVAVALIFAILKYRPSPFLVLDEVDAALDDANTNLFNRLIKDVAGKSQ
                     VVMITHNKSTMEVADTLFGVTMQKQGISTLVSVNLN"
     gene            complement(30811..31254)
                     /locus_tag="PLF77_00145"
     CDS             complement(30811..31254)
                     /locus_tag="PLF77_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPC07290.1"
                     /translation="MQRVSDILKSKSKQMWTINPNDTVFEALKLMAEKEIGALVVVDK
                     KGKVAGIITERDYARKIVLKGKTSPKTLVKEIMTPFNKMYTVKPDTSMEGCMVLMTGK
                     HIRHVPVFEGSKFLGIVSIGDVVKSTISEKDVLINQLSNYIAGKY"
     gene            complement(31408..33336)
                     /locus_tag="PLF77_00150"
     CDS             complement(31408..33336)
                     /locus_tag="PLF77_00150"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPC07291.1"
                     /translation="MRFEHLFSPFTINGMTLKNRAVMPPMATGYGNADNTVSERLLAY
                     LERRAQGGTGLIITEVCAVDPRGKGFPNEIGVWNDDFIGALSRIPAALHRHGAKAALQ
                     LHHAGRETFEAVLGKKPEAPSAIPSAVLGQPCEAMSLERIAEIVRAFAQAAGRARQAG
                     FDSVELHGAHGYLLNQFLSPFSNNREDEYGGSEENRMRFILEVIAAARKAVGDDFPLW
                     IRISADEMVRGGYDFSLMRRLAPRMVTAGVDAIHCSVGVYSTPGGLSIASMDTEPGFN
                     LFRARALKEVVSVPIIGVGRINDPAMADQAIARGDADLISFGRQHLTDPDFIEKARRG
                     SLEDIRRCTACNQGCIERLSFEMKSTTCSFNPECGREYKGPVLPAGTPKKVWVIGAGP
                     AGLSAALVLAARGHQVEVFEKESQPGGQLLSASRPPHKAVFMDWVNWALRRLKQWSVP
                     LHCSHPVSADDIKKAAPDAVIVASGAYPVTASIPGLKSELVADARDVLTGNIKLAGPA
                     VVLGAGYVGMETADYLLANGIAVTVLEMQTLPPVNRFSAHGYWLNKRIKDSGGKIIFG
                     ARVLGIENNIVRYVQADKPSEEKASLIVTAMGAKSENTLEDVLNALAVPYRVVGDARS
                     PRRILEAIHEGYKAGEEI"
     gene            complement(33615..33776)
                     /locus_tag="PLF77_00155"
     CDS             complement(33615..33776)
                     /locus_tag="PLF77_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07292.1"
                     /translation="MTEEIKQEQPATDPHKQENQPITLKEKIAAAVLIAYVISLIWLA
                     GSKHWFGLW"
     gene            complement(34090..35100)
                     /gene="amrS"
                     /locus_tag="PLF77_00160"
     CDS             complement(34090..35100)
                     /gene="amrS"
                     /locus_tag="PLF77_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPC07293.1"
                     /translation="MIKEAMLYEKLQDGNVRCNLCAHRCTIHPERQGVCGVRGNRDGV
                     LYSMAYGTLIAQNVDPIEKKPFFHVYPASRSYSIATMGCNFFCDFCQNHDISQAPRKT
                     KMIYGEDSMPSDIVERAKESGAKTIAYTYTEPTVFFELAYDTAKIAAASGLKNVFVTN
                     GFMTPEAIEIIAPYLSAANVDLKSFRDEFYKKRCGARLVPVLDSLKKMKERGIWVEIT
                     TLLIPSLNDSEEEMRDIAQFIAGLGRETPWHISRFHPQYKMQHLPVTPLESLHRAGQI
                     GREAGLKYVYSGNVPGDDAENTRCFHCGKLLIERYGFKIIGTNLQKNKCSHCGTQLEG
                     IF"
     gene            complement(35097..35879)
                     /locus_tag="PLF77_00165"
     CDS             complement(35097..35879)
                     /locus_tag="PLF77_00165"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC07294.1"
                     /translation="MFFDQKKTGFPAERLKTPRDDYIFSRQKELVLDLITPLAGERLL
                     DIGCGTGNHLDMFRGKWCSLTGLDSSGANLEIARQKHSERAELILADPEDIPFSDNEF
                     DIVTMIHCLETAVHPQKIIDEAVRVCRGRVFIGCVNNYSFVGTRQRLKEMFGFSFSQK
                     IRFFSLPEIRKMAEVSAGNNAMKWGSVIYFPAMVYDFFEELEEMLPFRKNPFGAFVGL
                     VCPVTYTYRTAQNPVMNSFNIKAETRKTAPEAVRNMLKEADR"
     gene            complement(36013..37008)
                     /gene="moaA"
                     /locus_tag="PLF77_00170"
     CDS             complement(36013..37008)
                     /gene="moaA"
                     /locus_tag="PLF77_00170"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HPC07295.1"
                     /translation="MMLDKYSREINYLRVSITDRCNLRCIYCRPKEGVALHGHEDILR
                     YEEMIRVISLAVKMGLIKVRLTGGEPLVRRGFVDFVADLNKIEGLKDISLTTNGILLE
                     QFAQGIFDAGIGRINVSLDSLNRDKYYEITRGGRLEDVLRGIARAEEIGFFPIKINTV
                     AIRGFNDDEALDFARLAEERPFQIRFIELMPVGKTDLDYDKDYLSGSELIKIIRQRYV
                     LEPVKNKHNRSDGPAKIFKIKGGRGEIGFINPVSDHFCSTCNRMRLTSDGKLRACLLN
                     DAEVDLKKALRENCSDAELEKLIWDAVLLKPRQHDIVCTENSLRKCSRNMSEIGG"
     gene            37180..37659
                     /gene="greA"
                     /locus_tag="PLF77_00175"
     CDS             37180..37659
                     /gene="greA"
                     /locus_tag="PLF77_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HPC07296.1"
                     /translation="MEKVPITKAGFEKLKKELETLKNVSIPENVRDIEVARAHGDISE
                     NAEYSAAKERQSFLYGRMQELENQLAMSNVISLKGLNTAKVVFGCYVSLQDIDSGEKI
                     KYQLLGPYESDISRNKISVTSPIGKALIGKNVGSEINVKTPGGTRNLEITDISVEEE"
     gene            complement(37670..38800)
                     /locus_tag="PLF77_00180"
     CDS             complement(37670..38800)
                     /locus_tag="PLF77_00180"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S double cluster binding domain-containing
                     protein"
                     /protein_id="HPC07297.1"
                     /translation="MLTKEEIIDEAKRLKFADIGFTGAEPFGSQKDYLLTHQEEYGWA
                     EAIGLGLLAGTDPRNILAGAKSIIVVLESYFEESFPQPMERHFGRCYLDDDRITQDGL
                     ALKIKAFRKYLKANGVNSKVPFHLPHRLSAARAGLGTFGNNCIFYARRVARGSSFVFP
                     IALVVDCEFTPDEPSVAMGCPDWCRNACVAACPTRALKGGGKIDPRRCISFLTYYGSG
                     LTPQDLREPMGMYIYGCDRCQNVCPRNIPWMAEEKPVNQRVSAKASDFGLSRLLHMDA
                     VYFEKKIWPHMFYMSPDDLWRWKMNVARAMGNSLNPVYVADLIRAFHENSDERVRCMA
                     AWALGRIGGNEAVEALGRFLKNSDGVVREEIEQAMVSASAGV"
     gene            complement(38758..39543)
                     /locus_tag="PLF77_00185"
     CDS             complement(38758..39543)
                     /locus_tag="PLF77_00185"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC07298.1"
                     /translation="MHLTGAMFHSRKYPTKDHYPFHLPLFRATKRLVFDTPVTLFVGE
                     NGTGKSTLIEALAQACQIHIWRDTQRTRPDYNPYEDQLHRYMSVIWANEPVPGSFLGS
                     KNFQYFAEKLDEWASADRDQLNYFGGRSLTTQSHGQSLMSFFYSRYRIPGLYLLDEPE
                     TALSPQSQILLLDILQRMSAAGHAQFIIATHSPLLLACPQATIYNFDRSPLTKIVYEK
                     TKHYRIYKDFLLNRSNYLKPKAKHQRKDTKCLPKKKSLTKQSA"
     gene            39730..40572
                     /locus_tag="PLF77_00190"
     CDS             39730..40572
                     /locus_tag="PLF77_00190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HPC07299.1"
                     /translation="MNAKSALIIAAIVFIFTGCASENIQVRKLPDSAINAGLEDLRQE
                     VADNGVPGEWFDDQMDHYAFRLHHNIDRFFRKSAEKKTDSEKKRSLLWYFSIFGVETK
                     IEKGKTFIEDYTGILERAEAKHGIHKELIAAIIGMETNFAQRRYRGKFYAFNSLVSQY
                     IFTDRKKFAVREITSLYKFSEKTDRSPWYFKSSYAGAIGWGQFIPSSLLSFFIDENGD
                     NDDIDPFSIEDTIFSVENYLYRHGLSGKNVHKRRSRYKAVYAYNHSDAYVQAVLHIYD
                     GLKN"
     gene            complement(40623..40832)
                     /locus_tag="PLF77_00195"
     CDS             complement(40623..40832)
                     /locus_tag="PLF77_00195"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPC07300.1"
                     /translation="MNFKIGLVAIFALLALIFLVQNIEVMTVHFLFWQLSMSRAIFLF
                     FILLTGFIIGWFLHSFVSYSQKKKN"
     gene            complement(40873..42246)
                     /locus_tag="PLF77_00200"
     CDS             complement(40873..42246)
                     /locus_tag="PLF77_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC07301.1"
                     /translation="MKKILIVDDELNMRLVLSAMLKKEGFEVSSASNGREALQILQSN
                     KMALVITDLKMPGMDGMDLLTQISANHPQIPVVMITAHGTIATAVEALKKGAFDYITK
                     PFDLDDLKNIVSKAIRTSTLKESEFSMAPSEIDRLGIIGTSPAIMEIFEAIHRVAQTT
                     TTVMITGETGTGKELVADAIHYNSQRKNNPLIKINCAAIAETLMESELFGYEKGAYTG
                     AAVTKPGKFELAHKGTLFLDEVAEIPREMQVKLLRVLQEQAFERVGGLRTIKVDVRII
                     AATNQHLLRQVQTGNFREDLYYRLNVFPIDIPPLRERKDDILSLADYFLDKFNKKLGI
                     SVGMDNEVKDILLRYDWPGNIRELENLIERMMLLARGCRIMSGDVPDDFKKSVDHVNT
                     ALSPGIKKPFKEYVRDQVENVERQMIARALDESGGNITQAAKKMGLSRKGLQLKMSKY
                     HLRKESK"
     gene            complement(42300..43817)
                     /locus_tag="PLF77_00205"
     CDS             complement(42300..43817)
                     /locus_tag="PLF77_00205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC07302.1"
                     /translation="MDTFILVATSFTIAVSLMITGKTNRLQSAFAALCAAVFVSQAAV
                     TLENIFHYEFLLKIKYLGSLAIAPAALRFFHHLTKGQSIILRNFFSLFAVISLCGVVF
                     VFLPLSEKTCFHNVIAAYVLFSLMICYLALLGHVGTLPSGTEKRRFGYLLVFCPVALI
                     LSYLDLLNFWGFSLLKINGMVSSALLYFILLIIAYPQLLELHDFLARSLVILLSTLAG
                     AAIFYFTVSFFNGDPPLTGLLMASFLVVISLSPMRMIFRKIFGVFYPDSKDIFTSLYE
                     FDEKLEREKALMLEEMAPVLAHEIRNPLGSIKGAAQYLKTEASTGEHKELLDVIVEGT
                     DRLNTVVSKFLDYARPYQLQLKTQDINAIIRRAISVIAANKLLEHITIVEDLEDKLPF
                     AAVDEQQCLQVVLNIALNAIEAMPQGGTLTFCTSSQKSETAEDLALTIRDTGTGIDQK
                     YVKDIFKPFFTTKERGVGLGLAICQKIIREHNGRISVQSIASQGTTVVIQLKRSG"
     gene            complement(43914..46256)
                     /gene="lptD"
                     /locus_tag="PLF77_00210"
     CDS             complement(43914..46256)
                     /gene="lptD"
                     /locus_tag="PLF77_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HPC07303.1"
                     /translation="MNINTLINVKPIKHNGLASFSGCLEPVFTFCLVAVIIILWSFPA
                     DCAGKQKSSAGKASPSIQKKNFPASVHTNEKSPVSIEADAMDYDHVSDLYRARGQVSV
                     FYSGASLYADEVELDNQNHVARAQGNTLLKIGEDTLKGERLWFNIEDKTGAAFNADAF
                     YARNHFRVRGNIIEKTGENTYFIDNPSATTCDGSNPDWEIAGSEMKVTIEGYGLMKNA
                     CFKVGSLPLLYSPYILFPAKTKRQTGFLLPYLAYSRDKDGIDVELPFFWAVSPWMDAT
                     FYQRFIEKRGFKEGAEFRYDFGNKSFGTFYGDYLEDTKQVTETEDDALRRDWQGMHKR
                     WSYFLHHQTRLDSQFYLRTDLKKVSDKWYFRDFSSYNYYREHFNSTSEDRFRNVSFEG
                     DKSLRYLDSTIRVYKGWSKYNVTGLMHAADDFAAVNNDQTLQRYPEIIFAGVKQPLLH
                     TPFYYELAGRYDYLYKKEGHKGHFADLSPSLSMPVNLSDYVRIIPQFTFKETFWSRDD
                     DENGLKEKTADRSVYNASVSFGSQLFRVFHLNGKHWEKIRHEIKPEIIYSYMPSISAE
                     DVPDYYLPALSPFGMPLASLSSDPLNGQNAVAWSLTNTLTARVKDQGGSLSYLEFFRL
                     KVFQAYDIDEARRDMARNAPERRPFSDMGIEFDFSPHKYFSFRMRDKYNVYSGWKQNS
                     YDLSLRDWRGDALLVAYRNTEGLVEEINLGVKAVITDHFYSTFVLKRDLQDARKIENS
                     LGFVYHTQCWSLGFDLSDTDDDVRFLFRISLAGFGKTADK"
     gene            complement(46253..47563)
                     /locus_tag="PLF77_00215"
     CDS             complement(46253..47563)
                     /locus_tag="PLF77_00215"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPC07304.1"
                     /translation="MKNSRTSQYLDSLNINKMRFGLGAITSVLSRLGHPQKSFKAVTI
                     TGTNGKGSTAAMIAAMLHKAGYCVGLYTSPHLIDVRERITVNGRKISRKDFDRIIVCV
                     RKKVVQPLTYFEFITAVAFEYFKTRKADIAVLEVGLGGRLDATNVCRPLVSVITNIAF
                     DHTAYLGRTLTSIAVEKAGIIKPHGLCVTGVAQKKILGLLQDICLQRGASLYRLNHDF
                     RLARQKNGSLLYEGVYSDVKNLTVPLRGEHQLANAALALAAVEMCARKGFPVSETAVR
                     AGLQNVRWEGRLEVLHISPWVLLDGAHNPAGIGALCRSLKKDFSFRHLILIFGVLADK
                     DYRKMLQKIVPLARVIILAPLKAQRAASVAAVSQTIRSLGRMPVVSQSVHQALELGLS
                     LAGSRDLICATGSFYLAGEVKQTFSKLVFCDNEKSKKVQGKKVS"
     gene            complement(47651..48475)
                     /locus_tag="PLF77_00220"
     CDS             complement(47651..48475)
                     /locus_tag="PLF77_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HPC07305.1"
                     /translation="MKSRIAEAIKLKYQPVALLWSVEKPADAMQFAEGKWGCVMWLAV
                     HAAKGRAAVADRKTFGCFGGGVGLGFGNQYKNFPGGEEGFCYFLSSGNARRPGGPEMA
                     AKVKPFMTRESHDNFLNGERYVQNPQLVEKFIRDLPLTDIPKPFVVFQPLSDVDQDGE
                     KPQTVIFFVNPDQLSALTVLANYGREGNENVIIPYAAGCQAIGIYPYREAGLKNPRAV
                     VGLTDLSARVYVRKQLGESHLMTFSVPFNLYDEMEQNVPGSFLERHTWQSLLSENR"
     gene            complement(48596..48856)
                     /locus_tag="PLF77_00225"
     CDS             complement(48596..48856)
                     /locus_tag="PLF77_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07306.1"
                     /translation="MAEKKIGEVMKFFSKPSVAAVKITDGEIAVGDSLKFSGHTTDFT
                     DVIESMEVDNQSVPKAVAGDFIGIKVSDRVRPGDEVFKVIPD"
     gene            complement(48878..49039)
                     /locus_tag="PLF77_00230"
     CDS             complement(48878..49039)
                     /locus_tag="PLF77_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07307.1"
                     /translation="MGFNGGELTVMTLPLALGRPGSLSMIQQTQALKTGNEAVLDIID
                     CPVRVYAFR"
     gene            complement(49176..49598)
                     /gene="rplQ"
                     /locus_tag="PLF77_00235"
     CDS             complement(49176..49598)
                     /gene="rplQ"
                     /locus_tag="PLF77_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPC07308.1"
                     /translation="MYHGKAGRKLGRTSSHKEAMLRNMVTSVIKHERIRTTDAKAKEL
                     RKLAEKMITLGKKGSLHARRQALAVIRDKETVGKLFGEMTERYRNRAGGYTRIVKAGY
                     RYGDNAPVSILEFINDEKKKEKAKPKARSRAKAKEKAE"
     gene            complement(49662..50630)
                     /locus_tag="PLF77_00240"
     CDS             complement(49662..50630)
                     /locus_tag="PLF77_00240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPC07309.1"
                     /translation="MQRNWSSLIRPKRIDVDEATHTNFYGEFTIQPLERGFGITLGNA
                     LRRVLLSSIQGSAIVSAKIEGVLHEFSTISGVKEDVTDIILNLKGVRFKLGQANIKEV
                     SIDVSRAGEVTAADIMTDGTVEILNPEHHIATLSSGSFKAKMMVKMGKGYVPAKKELD
                     PDQPKGTINIDAVFSPIKKVNYVVSYARVGQIADYDKLTLEVWTDGSVKPEDAIAYSA
                     KILKQQLDVFINFEEVEEEILPEKQDEPDNINKILSRSVEDLELSVRSANCLKNAGIN
                     TIGELVKKTEAEMLKTKNFGRKSLSEIKDILAEYDLTFGMDIGSKN"
     gene            complement(50676..51305)
                     /gene="rpsD"
                     /locus_tag="PLF77_00245"
     CDS             complement(50676..51305)
                     /gene="rpsD"
                     /locus_tag="PLF77_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPC07310.1"
                     /translation="MARYTDSSCRLCRREGLKLFSKGDRCYSEKCSFERRDYVPGDHG
                     QMHKKQRSDYGVQLREKQKLKRMYGLLEKQFRSYFEKADKQKGITGTNLLILLERRMD
                     NMVFRMGFANSRIESRQLISHGHFLVNGKPVNIPSYLLKAGDEVSVKENSRKVTRIMG
                     ALETVVRRGVPQWLELDKDNFNAKIKTLPVREDLTMPIQEQLIVELYSK"
     gene            complement(51353..51745)
                     /gene="rpsK"
                     /locus_tag="PLF77_00250"
     CDS             complement(51353..51745)
                     /gene="rpsK"
                     /locus_tag="PLF77_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPC07311.1"
                     /translation="MAKAVRKTGKKKEKKNITDGIAHIQSTFNNTIVTITDMSGNVIA
                     WSSSGLQGFKGSRKSTPFAAQMAAEDVVRKAKEQGMRKVQVYIKGPGAGRESALRSLQ
                     IAGLTITLIRDVTPVPHNGCRPPKRRRV"
     gene            complement(51772..52140)
                     /gene="rpsM"
                     /locus_tag="PLF77_00255"
     CDS             complement(51772..52140)
                     /gene="rpsM"
                     /locus_tag="PLF77_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPC07312.1"
                     /translation="MARISGVDLPKNKRMEIALTYIYGIGKTRAKQILKDAGVSPDTR
                     TDDLADSEISAIRGIIDKEGKVEGDLRRDISMSIKRLMDVGTYRGLRHRKGLPVRGQR
                     THTNGRTRKGPRRAIAGKKK"
     gene            complement(52166..52279)
                     /gene="rpmJ"
                     /locus_tag="PLF77_00260"
     CDS             complement(52166..52279)
                     /gene="rpmJ"
                     /locus_tag="PLF77_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HPC07313.1"
                     /translation="MKVRSSVKKICEKCKIVKRKGVLRVICENPKHKQRQG"
     gene            complement(52298..52516)
                     /gene="infA"
                     /locus_tag="PLF77_00265"
     CDS             complement(52298..52516)
                     /gene="infA"
                     /locus_tag="PLF77_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPC07314.1"
                     /translation="MAKEEAIEVEGRVIEPLPNAMFRVELDNGHKVLAHISGKMRMHF
                     IKILPGDKVTVELSPYDLTRGRITYRTK"
     gene            complement(52538..53290)
                     /gene="map"
                     /locus_tag="PLF77_00270"
     CDS             complement(52538..53290)
                     /gene="map"
                     /locus_tag="PLF77_00270"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPC07315.1"
                     /translation="MVILKLPEEIERARASNRIVAEVLSRLREKVKPGVKTKDLDKFA
                     EEIAEKRGARPAFKGYHGYPHSLCVSINEVVVHGMPSERVLEEGDIVGLDFGVFYQGF
                     FGDATVTLPVGKVTAKALRLMQVTEQSLYAGIAQAREGNRLGDISAAVQSVVEEAGYS
                     VVRDFVGHGIGKDLHEEPQIPNFGQKGRGIELKKGMILAIEPMVNEGKYKVQVLPDGW
                     TVVTKDGSLSAHFEHSIAITEHGPDILSRLNY"
     gene            complement(53303..54610)
                     /gene="secY"
                     /locus_tag="PLF77_00275"
     CDS             complement(53303..54610)
                     /gene="secY"
                     /locus_tag="PLF77_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPC07316.1"
                     /translation="MLEGLGSVSKIPELQRRILFTILLLGVYRIGVYIPTPGIDNVAL
                     LAYFEHAKGSLFGLMDMFAGGAFSNFSIFALGIMPYISSSIILQLLTIAVPHLERLSK
                     EGESGRRKITQYTRYGTVLLSMIQGFGIAFGLQQMAGPSGAPIVLNPGIGFVLMTMIT
                     LTSGTAFIMWLGEVITEKGIGNGISLIIFAGIVCRIPAAIGNTFRLATAGELGVLVIL
                     LITVLMIAVVGAIVFVEGGQRRIPVQYAKRVVGRKMYGGQTTHLPLKVNTSGVIPPIF
                     ASSVIMFPATIANFAPQTWMKDIAAYLVPGRLAYEVLYVAFIFFFCFFYTAVTFKPDD
                     VAENMKKYGGYVPGIRPGKKTAEYIENVLEKLTFSGAIYVSIICVLPSVLIAQLNVPF
                     YFGGTALLIVVGVAMDTASQIESHLLTRQYEGFMKKGHIARRR"
     gene            complement(54612..55058)
                     /gene="rplO"
                     /locus_tag="PLF77_00280"
     CDS             complement(54612..55058)
                     /gene="rplO"
                     /locus_tag="PLF77_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPC07317.1"
                     /translation="MDLGSLKAPAGATKKRKRVGRGNASGHGGTSGKGHKGQKARSGG
                     KVRVGFEGGQMPMARRLPKRGFRNPFRKEYIAVNILDLCRKFSKGAVIDEAALLQSGL
                     IKTEGAHIKILAKGDIDFPVTLKIARISPAAKDKIIAAGGSVEEVI"
     gene            complement(55062..55241)
                     /gene="rpmD"
                     /locus_tag="PLF77_00285"
     CDS             complement(55062..55241)
                     /gene="rpmD"
                     /locus_tag="PLF77_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001085235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPC07318.1"
                     /translation="MLKITKVKSVIGRPEKQRKILKGMGLTKLNKTVTLEDTPQIRGM
                     INKVTHLVSVEESKE"
     gene            complement(55282..55746)
                     /gene="rpsE"
                     /locus_tag="PLF77_00290"
     CDS             complement(55282..55746)
                     /gene="rpsE"
                     /locus_tag="PLF77_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003133760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HPC07319.1"
                     /translation="MKDMELLDRVVAINRTAKVVKGGRRFRFSAIVVVGDGKGMIGTG
                     LGKAHEVPEAIRKGMDMAKKNMVRVAVVDRTIPFEVIGRYGAGKVLLKPASEGTGLIA
                     GGAVRAVLEVAGVHNVLTKCLGSHNPHNLVKATFEGLCQLKNPEEIAAQRGK"
     gene            complement(55804..56169)
                     /gene="rplR"
                     /locus_tag="PLF77_00295"
     CDS             complement(55804..56169)
                     /gene="rplR"
                     /locus_tag="PLF77_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HPC07320.1"
                     /translation="MASKKTLKIREKREKRTRSKIKGTQERPRLSVFRSDRHIYVQAV
                     NDVTADTLATASTLCKELESRLKGKKKVDVAKEVGKLIAERLKAKGIEKVVFDRGRFL
                     YHGRVKALAEAARESGLKF"
     gene            complement(56208..56747)
                     /gene="rplF"
                     /locus_tag="PLF77_00300"
     CDS             complement(56208..56747)
                     /gene="rplF"
                     /locus_tag="PLF77_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HPC07321.1"
                     /translation="MSRIGKKPVSIPKNVKISQSADTVKVEGPKGALSSRMPEGIAIK
                     ISDGTLSVERKSEALTTRSYHGLTRTLIDNMIKGVTTGFEKRLEISGVGYRAEANDKS
                     LKFILGFSSPVQYDIPKGIDIKIDKQVNIVVSGIDKELVGRVAAEIRALRKPEPYKGK
                     GIKYANEYIKRKAGKSAGA"
     gene            complement(56783..57184)
                     /gene="rpsH"
                     /locus_tag="PLF77_00305"
     CDS             complement(56783..57184)
                     /gene="rpsH"
                     /locus_tag="PLF77_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HPC07322.1"
                     /translation="MGMTDPIADMLTRIRNANKARFKTVRVSMSQVNVNVAGVLKKTG
                     YITNYEKVKDEKGRQMLEIILKYPDAKRTVITNIKRVSKPGRRIYVPADNIPKVLNGL
                     GISILSTSRGVITDAEARELNVGGEILCNVW"
     gene            complement(57209..57394)
                     /locus_tag="PLF77_00310"
     CDS             complement(57209..57394)
                     /locus_tag="PLF77_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HPC07323.1"
                     /translation="MAKKSLIAKAKREKKFSVREYNRCPLCGRPRAYYRKFDMCRICL
                     RKLSLQGELPGVIKSSW"
     gene            complement(57421..57960)
                     /gene="rplE"
                     /locus_tag="PLF77_00315"
     CDS             complement(57421..57960)
                     /gene="rplE"
                     /locus_tag="PLF77_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HPC07324.1"
                     /translation="MARLKEYYSKTVAPALVQEFKYKNPMQVPKMEKIVINMGLGEAI
                     QNVKIIDGAVQELAAITGQKPIVNKAKKSIATFKLRQGMPVGCSVTLRKKIMYEFFDR
                     LVNAALPKVRDFRGISPHAFDGRGNFSIGLQEQIIFPEIDYDKIEKNKGMNITIVTTA
                     KTDEEARFLLKMMGVPFKQ"
     gene            complement(57976..58290)
                     /gene="rplX"
                     /locus_tag="PLF77_00320"
     CDS             complement(57976..58290)
                     /gene="rplX"
                     /locus_tag="PLF77_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HPC07325.1"
                     /translation="MSLNKFKKGDTVKVLAGKDKGKTGKVLAVIPEKDRIVVEKVNVI
                     KKHKRPDQRSKGGIVEKEGSIHVSNVGLFCNKCNKPVRVQSKKLEDGKKVRICSKCKD
                     VI"
     gene            complement(58313..58681)
                     /gene="rplN"
                     /locus_tag="PLF77_00325"
     CDS             complement(58313..58681)
                     /gene="rplN"
                     /locus_tag="PLF77_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HPC07326.1"
                     /translation="MIQMQTILNVADNSGAKKVACIKVLGGSKRRYASLGDVIVVSVK
                     EAIPNSKVKKGDVIKAVIVRTVKEVRRPDGSYLKFDDNSAVLISNQMEPVGTRIFGPV
                     ARELRARQFMKIISLAPEVL"
     gene            complement(58727..58984)
                     /gene="rpsQ"
                     /locus_tag="PLF77_00330"
     CDS             complement(58727..58984)
                     /gene="rpsQ"
                     /locus_tag="PLF77_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HPC07327.1"
                     /translation="MDQRGFNRTIKGVVVSNKMDKTIVVRAERFVKHPVFHKYVRKHV
                     KYKAHDERNQSKIGDTVLIIESRPLSREKRWRMLEILEKAK"
     gene            complement(58986..59180)
                     /gene="rpmC"
                     /locus_tag="PLF77_00335"
     CDS             complement(58986..59180)
                     /gene="rpmC"
                     /locus_tag="PLF77_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HPC07328.1"
                     /translation="MKIKEIRALDSNGLKQKIAELSEELFRLKIRHASGQLESTVMLS
                     KIRKDIARVNTVLKEKEAAS"
     gene            complement(59170..59592)
                     /gene="rplP"
                     /locus_tag="PLF77_00340"
     CDS             complement(59170..59592)
                     /gene="rplP"
                     /locus_tag="PLF77_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HPC07329.1"
                     /translation="MLMPKRVKYRKLQKGRMGGKATRGSSIAFGEYGLQAAECGWITS
                     RQIEAARVAMTRHVKRGGRIWIRVFPHKSVTKKPAETRMGKGKGAPEGWVAVVKPGSV
                     LYEMEGVSKDVAREAFRLAAHKLSIATKFLSRDIVDEN"
     gene            complement(59671..60312)
                     /gene="rpsC"
                     /locus_tag="PLF77_00345"
     CDS             complement(59671..60312)
                     /gene="rpsC"
                     /locus_tag="PLF77_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HPC07330.1"
                     /translation="MGQKVNPVGLRLGYIKKWQSVWYTDKKYSDVLHEDLQIRKYLKK
                     KLYHAGLSKIEIERAANKAKVNIYSARPGVIIGKKGSEIEKLKSEIERFSQSEIIINI
                     LEVRKPEVDAQLVAENVAMQLEKRVAFRRAMKKCVGYALKFGAKGIKIACSGRLGGAE
                     MSRSEWYREGRVPLHTLRADIDYGFTEAKTAYGLIGVKVFIFHGEVLPTKNEK"
     gene            complement(60324..60656)
                     /gene="rplV"
                     /locus_tag="PLF77_00350"
     CDS             complement(60324..60656)
                     /gene="rplV"
                     /locus_tag="PLF77_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPC07331.1"
                     /translation="MEAKAVAKYMRMSPQKVRLVADLIRGKNVQEARNILLYTRKYAA
                     GVVANVLKSAVANAAQNPNIDENILYVKEIYVDQGPALKRWRARAQGRAAGIKKRSSH
                     ITVILDEI"
     gene            complement(60699..60977)
                     /gene="rpsS"
                     /locus_tag="PLF77_00355"
     CDS             complement(60699..60977)
                     /gene="rpsS"
                     /locus_tag="PLF77_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPC07332.1"
                     /translation="MARSIKKGPYIEEKLLAKIRKMEAEGTKNVIKTWSRRSTITPEL
                     IGYTFAVHNGKKFIPVYVTENMVGHKLGEFAPTRVFFSHSGDRKTKVS"
     gene            complement(60980..61804)
                     /gene="rplB"
                     /locus_tag="PLF77_00360"
     CDS             complement(60980..61804)
                     /gene="rplB"
                     /locus_tag="PLF77_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPC07333.1"
                     /translation="MAIIKYKPTSPGRRFQSVSDFAEITSTEPVKRLLKPLKKKGGRN
                     SYGRVTARHIGGGHKRKYRVIDFCRNKADIPAKVASIEYDPNRTARIALLNYRDGEKR
                     YIVAPAQVNVGDVLYSGEKADIKPGNAVPLKYIPLGSLIHNVELKVGRGGQLIRSAGA
                     YGQLMAKEGSYAQVRLPSGEVRKIFIECMATIGQVGNNEHENISIGKAGRTRWLGKKP
                     HVRGVVMNPVDHPMGGGEGKSSGGRHPCTPWGIPTKGHKTRKNKRTDKYIIKRRGK"
     gene            complement(61832..62119)
                     /gene="rplW"
                     /locus_tag="PLF77_00365"
     CDS             complement(61832..62119)
                     /gene="rplW"
                     /locus_tag="PLF77_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPC07334.1"
                     /translation="MDRSKVVKRILVTEKSTMARDASNKYFFEVDRKANKVEIADAVE
                     KLFKVKVMDVRVMHVLGKKKRMGRTVGQKSSWKKAVVTLAAGNSIEFAEGV"
     gene            complement(62119..62742)
                     /gene="rplD"
                     /locus_tag="PLF77_00370"
     CDS             complement(62119..62742)
                     /gene="rplD"
                     /locus_tag="PLF77_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPC07335.1"
                     /translation="MAVADVLNIEKKKVAEVDLNDAVFGADVNEAVIYDVVKMQLASR
                     RSGTASTKTRSDVSGGGKKPFRQKGTGRARAGSSRSPVWRTGGTVFGPHPRDYSFSVP
                     KKIRKKALISALSLKFKENKMIILKDFPMEKISARGFQKVVDLFDLKKALFVLDDNNE
                     ILMKSSRNIKNVKMIRSEGINVYDVLNHEHLILLEPSVKKIEGALLV"
     gene            complement(62771..63397)
                     /gene="rplC"
                     /locus_tag="PLF77_00375"
     CDS             complement(62771..63397)
                     /gene="rplC"
                     /locus_tag="PLF77_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPC07336.1"
                     /translation="MSKGIIGKKLGMTQVFADDGSAVGVTAIEVEPSVVLQVKTKDKD
                     GYDAVQLGYGRVKQKNVTRPLQGHFNKANKGFFRFVREFPAHSETYEVGQEISADIFA
                     MGDYVDVVGTSKGKGFQGVVKRHGFGGGRATHGSMFHRAPGSIGASADPSRVFKGTKM
                     GGHMGNVRKTVQNLKVWQVRSERNLLLLEGSVPGSRNSFLLITKAKKK"
     gene            complement(63486..63794)
                     /gene="rpsJ"
                     /locus_tag="PLF77_00380"
     CDS             complement(63486..63794)
                     /gene="rpsJ"
                     /locus_tag="PLF77_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPC07337.1"
                     /translation="MKEQKIRIRLKAYDYKLLDRSVEEIVETAKKTGARVAGPIPIPT
                     EINKYCVNRSPHVDKKSREQFEIRTHKRLVDIVGPTQATVDALMKLDLSAGVDVEIKA
                     "
     gene            complement(63814..65007)
                     /gene="tuf"
                     /locus_tag="PLF77_00385"
     CDS             complement(63814..65007)
                     /gene="tuf"
                     /locus_tag="PLF77_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HPC07338.1"
                     /translation="MAKKKFERTKPHLNIGTIGHVDHGKTTLTAAITKYLAKKGFAEF
                     RAFDSIDNAPEEKERGVTINISHVEYETEKRHYAHVDCPGHADYIKNMISGAAHMDGT
                     ILVVAASDGPMPQTREHILLARQVQVPYIVVFLNKVDLVDDPELLDLVELELRELLTQ
                     YEFPGDNIPIIRGSALKALEADDINSPDVKCIAELMDAVDNYVPEPKRDTDKPFLMPV
                     GDVFTISGRGTVVTGRVDRGIVKVGEEVEIIGIRETIKTVVTGVEMFRKTLDEGRAGD
                     DVGLLLRGIKREEVERGQVVAKPASITPHTKFEAAVYVLTKEEGGRHTPFFSGYRPQF
                     YFRTTDVTGVATLPEGVEMVMPGDNVKMSIELITPIAMEEQLRFAIREGGRTVGAGVV
                     SKVIA"
     gene            complement(65064..65534)
                     /gene="rpsG"
                     /locus_tag="PLF77_00390"
     CDS             complement(65064..65534)
                     /gene="rpsG"
                     /locus_tag="PLF77_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HPC07339.1"
                     /translation="MPRRREVEKRDILPDPKFNNKLVAKFINSLLKRGKKSVAESILY
                     GAFDIIEKRVKEQPVDFFEKAVNNVKPVIEVKSRRVGGSTYQVPTEVVPARRTALAIR
                     WLISNAQERTEKTMREKLASELIDAANNRGGAIKKKEAVHKMAEANKAFAHYKF"
     gene            complement(65549..65920)
                     /gene="rpsL"
                     /locus_tag="PLF77_00395"
     CDS             complement(65549..65920)
                     /gene="rpsL"
                     /locus_tag="PLF77_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HPC07340.1"
                     /translation="MPTISQLVRKGRAKIKRKTNSPALAGSPQRRGVCVRVYTTTPKK
                     PNSALRKVARVRLTSGYEVTSYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHVIRGTL
                     DTVGVQDRKQGRSKYGAKKPS"
     gene            complement(66032..70126)
                     /gene="rpoC"
                     /locus_tag="PLF77_00400"
     CDS             complement(66032..70126)
                     /gene="rpoC"
                     /locus_tag="PLF77_00400"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPC07341.1"
                     /translation="MEDVFSYFEKPKNLVKFNAMQISIASPDKILSWSHGEVKKPETI
                     NYRTFKPEKDGLFCAKIFGPVKDYECLCGRYKRMKHRGVVCEKCGVEVIQSKVRRERM
                     GHITLATPVAHIWFLKSLPSRIGNAVDLSLKELEKVLYFESWIVLDPKNTPLKKKDLL
                     SDEEYEEAREQYGNDGFVAGIGAEAIRQLLEEIDLEKLYEELRTEIVTSVSDTKKKKL
                     VKRLKVVEALRKSGNKPEWMILTVLPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLKRLQELNAPEIIIRNEKRMLQEAVDVLFDNGRRGRAITGTNKRPLRSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVVTVGPDLRLHQCGLPKKMAIELFKPFIYNRLLEKGYV
                     TTVKSAKKMVEKESAEVWDALDEVVREYPIILNRAPTLHRLGIQAFEPVLIEGKAIQL
                     HPLVCTAFNADFDGDQMAVHIPLSVEAQTEARVLMMSTNNILSPANGSPIIIPSQDIV
                     LGIYYLTRAKTGVKGDGMIFAGPDEVRAAIDNGVIDLHARIKVRIDSELKETTAGRII
                     LSEIVPEQISFDDINKLMNKKELTSLIDHCYRLCGDKTTIILADKLKDLGFKYATVSG
                     LSFAVGNMIIPEQKKKIVLQADKDVLKIQRQYMDGLITDGERYNKVVDIWAQATEKIA
                     SEMLKGISTEDVPAAEGKKRKIESFNPIYMMADSGARGSGQQLRQLAGMRGLMAKPSG
                     EIIETPITANFREGLTVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDCIIT
                     EHDCETVDGVYVSALMEGGEIIETAGERVLGRVALQDVKDPFSGEVIVPANQMIDETL
                     AEKIDNAGIEKVHIRSVLTCRSKHGVCAMCYGRDLAHGHLVNIGEAVGIVAAQSIGEP
                     GTQLTMRTFHIGGTAKFDEHSTLEARHDGIVKFVNLSTVKTKEGDLVVMKRNGEVHVL
                     DEQKHVRGKYVLSYGAHLKVTDNSEVKKGQLIAEWDPFSIPILAEVDGTIKFDDITEG
                     KTMQEQVDEVTGLSRKIIVEFKGGDLRPRVSIKDEKGKTAKLADSNAVARYLLSVGVN
                     LVVSEGDQVRAGDIIAKIPRETTKTKDITGGLPRVAELFEARKPKDFAVISEISGVVS
                     YGKDAKGKRKIVVTPEVGEEKEYLIPKGKHISVQEGDVVSPGDALMSGVNNPHDLLSI
                     KGEKELARYLVDEVQEVYRLQGVKINDKHMEVIVRQMLRRVKVMDPGDTAFIADEKIE
                     RYRFQEENEKIIAQGGRPAIGEPMLLGITKASLSTQSFISAASFQETTKVLTEAALSG
                     KTDYLRGLKENVIMGRLITAGTGLVMYRKLGIALENENAETAAE"
     gene            complement(70207..74337)
                     /gene="rpoB"
                     /locus_tag="PLF77_00405"
     CDS             complement(70207..74337)
                     /gene="rpoB"
                     /locus_tag="PLF77_00405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPC07342.1"
                     /translation="MGDFRKNFGQVRKILDIPNLIDIQTESYEKFLQKDIAPVKRGAF
                     GLQGAFKSVFPISDFSGKCSLEFVSYKLGDIRYDVKECVQKGMTYAAPLKIVVRLVVF
                     EADRPAEGKGAGETTEAKQIRDIKEQEVYFGEIPLMTENGTFIINGTERVIVNQLHRS
                     PGIFFDHDKGKIVASGKLIYSARVIPIRGSWLDLEFDSKDILYVRIDRRRKMPVTILL
                     KALGNSTEFILNYFYNVNTVINDNKKIYFAVDESLVGKKSPEDIKDSKSSELIVKKGR
                     KLTKPLIKRIMEAGVKRTAINENELIGKIPSRDVHDPETGEIVFRCNEELPANCLDII
                     REKNIRELKLIHIDEDMDNASIRDTLLLDKIDTTEDAIMEIYRRLRPSNPPTPETADK
                     FFKSLFFEPETYDLSNVGRAKMNYKLHMNVPTDNTVLSNEDILSAVKYLIDLKNGVGD
                     CSVDDIDHLGNRRVRSVGELIENQYRIGLVRMERAIKEKMSLQDIETMMPHDLINAKP
                     VSAVVNEFFNSSQLSQFMDQTNPLSEITHKRRLSALGPGGLTRERAGFEVRDVHPTHY
                     GRICPIETPEGPNIGLIVSLSTYARVNEFGFIETPYRKVENGRVSDDVHFVTAIEEEN
                     QIIASADRPLSKNGKFAEELISVRKGGSYISVEPEEIKMMDVSPTQLVSVAAALIPFL
                     EHDDANRALMGSNMQRQAVPLMNPEVPVVGTGVESVVARDSGAVVLAKRSGVVESVDA
                     SRIIVRADRMDEDGIDSGVDIYNLAKYQRSNQDTCFNQKPIISVGQRVHKKDILADGP
                     AIDNGELALGRNVMVAFMSWGGYNYEDSILVSERVVKEDIFTSIHIEEFETMSRDTKL
                     GKEEITRDIPNVGEEVLKNLDESGIVCIGVSVKPGDILVGKITPKGETQLSAEEKLLR
                     AIFGEKASDVRDTSLRVPPGVEGTVIDAKIFTRKGAESDSRSQAIEADAIEQIAKDRD
                     DEIRILTETIKKDITKLVVGKTAATRIGDAKKKTVLKKGDKITKEIWNDLPFSSWKDI
                     TIAENDGSEEKIAGLIASLERKIDFVSAHFEEKMEKIKASDELPPGVIKMVKVYIAIK
                     RRLSVGDKMAGRHGNKGVLSRILPEEDMPYFADGSTVDIVLNPLGVPSRMNVGQILET
                     HLGWAAKSIGEGLNELLEQQRAGLSHIKDELKKVYSSPDFDKFIDQASTEDIKRFVGR
                     LKKGMLVASPVFDGCPEDQIREMLKRTDLPETGQSILFDGRTGEPFDQEVTVGIIYMM
                     KLHHLVDNKIHARSIGPYSLVTQQPLGGKAQFGGQRLGEMEVWAMEAYGAAYALQEFL
                     TVKSDDVAGRTRIYESIVKGEHNFESGLPESFNVLVKELQGLGLDVELVEESDSLS"
     gene            complement(74377..74766)
                     /gene="rplL"
                     /locus_tag="PLF77_00410"
     CDS             complement(74377..74766)
                     /gene="rplL"
                     /locus_tag="PLF77_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014167065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HPC07343.1"
                     /translation="MSDKITKEDVVKFIEEMTVLELSALIKELEDKFGVSAAAPVMAA
                     VAAPGAAAAAGPVEEKTEFDVILTGFGAEKIQVIKVVRAITNLGLKEAKDLVEGVPKP
                     IKEGASKDEAADIKKKIEEVGGTVEIK"
     gene            complement(74792..75316)
                     /gene="rplJ"
                     /locus_tag="PLF77_00415"
     CDS             complement(74792..75316)
                     /gene="rplJ"
                     /locus_tag="PLF77_00415"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HPC07344.1"
                     /translation="MDRKTKEQIVSELQKKLKEANLGILTSFSGMNVEKMEALRNELR
                     KSNAEWKVVKNTLLRIASEGTDFSILADHFKWPVAIVLSYKDPVAPAKVLIDFAKKNA
                     ELEIKVGVLDKKLLTRNDLTVLAELPGKDVLLGKLVSVMAAVPTSFVRVLNGVPGSFV
                     QVLNAYCDKKKSLN"
     gene            complement(75638..76336)
                     /gene="rplA"
                     /locus_tag="PLF77_00420"
     CDS             complement(75638..76336)
                     /gene="rplA"
                     /locus_tag="PLF77_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HPC07345.1"
                     /translation="MLRRGKRILAAKAKVEPAKKYTLKEAAEIVVSTAGTKFDETVDA
                     AIRLGVNPAHADQMVRGSVVLPHGLGKTVRVLVFAKGEKEKEALEAGADLVGNDEVIE
                     KIKGGWMEFDRVIATPDMMGSVGKIGKILGPRGLMPNPKVGTVTFDVANAVKELKAGK
                     VEFRVEKAGIVHSPVGKVSFGAEKLRENVSALLETILKLKPASSKGTYIKSISISSTM
                     GVGVRIDPLDIKAA"
     gene            complement(76356..76781)
                     /gene="rplK"
                     /locus_tag="PLF77_00425"
     CDS             complement(76356..76781)
                     /gene="rplK"
                     /locus_tag="PLF77_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HPC07346.1"
                     /translation="MAKKIIANIKLQIKAGKATPSPPIGPALGQHGVNIMEFCKAYNA
                     MTQSQEGMIIPVVITVYADRSFTFITKTPPVSVLLKQAAKIAKGSSDPKRDKVGSVTA
                     SQVKDIAELKYKDLNAVNIEGAINIIAGTARSMGIEITG"
     gene            complement(76837..77367)
                     /gene="nusG"
                     /locus_tag="PLF77_00430"
     CDS             complement(76837..77367)
                     /gene="nusG"
                     /locus_tag="PLF77_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HPC07347.1"
                     /translation="MAFKWYVVHTYSGYENKVKISLEERIKTTGTQAYFSDILIPEED
                     VVELISGEKKSSKRKFFPGYILVRMDMNDEAWHVVKDTPKVTGFIGSKDKPSPIPDKD
                     VEHLKIRIDEGTLKPKPKFKFDVGDHVKIIDGPFTNFDGIIDELKPEKSKLRVIVSIF
                     GRPTPVELDFIQVTQG"
     gene            complement(77372..77587)
                     /gene="secE"
                     /locus_tag="PLF77_00435"
     CDS             complement(77372..77587)
                     /gene="secE"
                     /locus_tag="PLF77_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HPC07348.1"
                     /translation="MEDFKEKVKVTIQKITQFLKDAKMELKKVTWPTPKQALASTAVV
                     IILVFIVSLILGIIDFALAKAVKLILG"
BASE COUNT        19466 a        20140 c        17174 g        20792 t
ORIGIN      
        1 ttatatataa tacggtcaac acaatgacca aggtaattat atgtacattg cacaaaaaca
       61 attggaaaag ttaagaaatc tggccgttcc gggcaaggtg gttgttattt acggcgccag
      121 gcgcacgggt aaaacgacac tgctgaaaga atttctgaaa gacgaaacaa aagatcatct
      181 gctggtcaac ggagaagata ttaccgtcca ttcctattta tccagccagt cggtggaaaa
      241 gctcacatct tttgtcggca acgcaaaact tctgattgtt gacgaagcgc agaaaattcc
      301 caatatcgga ataaatctca agctgattgt tgatcatcta tccggtatcc gcatggtcgt
      361 caccggctcg tcgtccttcg atctggcgcg tcacacgggc gaaccgctga ccggacgaaa
      421 atacacgctc aaattgtttc cgctggcgca aatggaaatc ggtcagattg aaaaaataca
      481 tcaaactcaa gcccatctgg agaaccgcct gatttacggt tcttatcccg aagtcgtctt
      541 aatgcaggat aatcaagggc gggaaaagta tctcaaagaa atcgtgtctt cttatttgta
      601 taaagatatt ctggaactcg acggcatcag acaatccgcc aagattatcc gcctgctcca
      661 gcttcttgct ttccagatag gcaaggaagt atcactgacc gaattgggaa catcattatc
      721 catgagcaag aataccgtgg agcgctatct ggacctcctg gaaaaagcat tcgtcctgca
      781 tcgcttgacc ggtttcagcc gcaacctgcg cagtgaaatc ataaaaaact gccgctacta
      841 ttttctggac aacggcgtgc gcaacgcgct gatcaacaat ttcaatccgc tctcgcaacg
      901 caacgattgc ggcgaattgt gggagaatta cctgatcatg gaaagattga aaaggcagga
      961 atatctggac accccggcta ataattattt ctggcggact tataccagac aggaaattga
     1021 ttttgtcgaa gagcgtcaag gccgcctgtt cggctatgaa atgaaatggg gcaaggcaaa
     1081 acccaagcca ccgaaactct ggcagtcggc ctatccggaa tcttcctatg agctgattaa
     1141 cagagagaat tatctcaagt ttattacttg atgcaggaac tgctttcccg aacagttcca
     1201 taactaaaat accggagcaa atacttgtta gggacgtctg aaaaaatcag ttttatagtg
     1261 gataaaagcc agagcgggca gaggctgatt tttcagtggg acaaacattt caatatttaa
     1321 tctaaaagaa caaggtgttg acatggcaac tgttaaacga attaaatctg ataatgtatt
     1381 tcaattaaaa ataactctta atcatattaa gccaccgatt tggagaagag tattggttga
     1441 ttcagatatt aagctgcctg atttacataa aattatacag actgtaatgg gctggactaa
     1501 ttctcattta catcaattta ttattggcaa tcaatattac tcacttccaa gcgatgaatc
     1561 attttacaaa gtagttgatt atcgccgaat taaattggat tcattattca atacgccaaa
     1621 atcaaatttt atttatgaat atgattttgg ggatggttgg gaacattcaa tcgttattga
     1681 aaaaatactg cctcgtaaaa aaaacactta ttatccaata tgcattgacg gcaaacgaag
     1741 ttgcccacct gaagattgtg gtggcacctt tggttatgaa aatcttatta cgataataaa
     1801 caatcccgaa catgaagaat atgatgaaat gatggaatgg cttggagatt attttgaccc
     1861 gaaggagttc aatatagatg aagtgaatga attcctccgt gaaaaaaatt ttggttgtgt
     1921 cgaattattt gactaaaaga aggtattgat aatttataat atattcgttt ctatttttat
     1981 ggaaacatgg aacgcctttg agttatcaag tttttcaaaa tgaaaaagac tggattcccg
     2041 ccttagcggg aatgacaaaa ttgaggcaat aagatggtcg gacaggcgga aaaaataaaa
     2101 tttacagttg ataaaagcca gggcgggcag aggctggata tatatctcgc gcagagcgag
     2161 gcggcgcttt cgcgttcgca ggtgaagtac gccattgagg aaggcgatgt gctggtcaac
     2221 gataaagttc ccaaagtcag ccagcacctc aaggaaggcg acgtgattgt gctgaccaaa
     2281 aggccgccgg tggaggccat agccgtcccg caggaaatgc cgctggacat cgtttacgaa
     2341 gacgacgccg ttatcgtcat caacaaaccg gcgggtatgg tggtgcatcc ggcgcccggg
     2401 aatcccgata aaacgctcgt caatgctttg cttttccact gccatgattt atccggcatc
     2461 ggcggtgtct tgcgtccggg cattgtgcac cggctggata aggacacgtc cggccttatt
     2521 gtggcggcaa aatccgatga cgcgcaccgc aatctttctt cgcagtttga aaaacacgat
     2581 gtgcacaaaa aatatctggc gctcgtctgg ggagacgtga aaggcaatag tggagaaata
     2641 gttctgcctg tcggcaggca cacaaacaac cgcaaaaaaa tgtccacaaa aagcagacac
     2701 ggcaaaggcg cgctcactct ttggaaagtg cgcgaacgct tcggcgtggc aacgcttctg
     2761 gatgtggaga ttaaaaccgg acgcacgcat cagatccgcg tgcatttgtc cgaccgcggt
     2821 tatccggtgg tgggcgatgc cgtttacggc aactccgcca aaatcaacac ggttaaagat
     2881 cccctgctga aggcaaagat caaggaattt aaccggcagg cactgcacgc ggcacaactt
     2941 tctttcattc atccgcaaaa aggcgagcgg gtggtattta ccgcggacat gccgcaggat
     3001 ctggcggatt tgtgcgctca attacggaca agcatcaagt cagttgactc aaaaaaagat
     3061 atgtggaagg ataaattaaa agggtaatcc atgtttcaac tgaacaaaaa gcattccatc
     3121 cagtattacg aatcctcgtt tttgagcgga tgcgattttt tgacgcatgc cttctgcacc
     3181 aggctcggcg gtctctcgga agacgattat aaatcgctga acatcagttt caaggaaggc
     3241 gatctggaaa gcagggtttt gcagaactgg aatcgtctgg ccatggcctt tgccataccc
     3301 ctggaaaact ttctgacatt gaatcaggtg cacggcgacg atatttttat aatcaaaccc
     3361 tacggcgatt atttaccttc ggacggagcc ctgaattacg acgctattgt aacgccccgt
     3421 gagaacctgg cgatctgcat caaaaccgcg gattgcgtgc cggtgttcat cgttgaccgg
     3481 gcaaaaaaaa tcattgcggc agtccatgcc ggatggaaag gcaccgcgct ggaaatcagc
     3541 gccaaagtcg ttcgtctttt cctggacaaa tacggatcat atccgcacga tattctggcg
     3601 gccgttggcc cctcgatcgg actctgctgc tttgaagttg atgaagacgc ggcggattct
     3661 tttcgcaatc aaaaagatca cgaagcgttt ttattcgcag gcaatagacc gaataaatgg
     3721 atcgtggatt taccggaagc caaccgcaga cagatgatca actgcggcat ttccgcagcc
     3781 aatatcgacc tttcccattt gtgcacgtcc tgccgtcagg atttgtttta ctcgcaccgg
     3841 ggttccggcg ggatcacagg ccggcagctt aatttcatca tggtgaaagg tgaggcaccc
     3901 tgccgcgtca tgaccatcgg cgacgaatat ccaaagattc agtaaaaaac atggctattg
     3961 acagtatttc ttttcagtat taattaagat catcgtcttc aaaaaacgat ttcgtttcta
     4021 aatcaatgat gatatcgcgg cggctaggag gaggcgacga gacgtattgt acatacgttg
     4081 aggaagccga caacgacgcc aacaaagata gcgcttgatt ttgaaatgaa ataaaggagt
     4141 gaacaaatga ttatcgttac aggtggcgcc ggatttatcg gcagcgcttt tgtctggaaa
     4201 ctcaaccagg aaggcattga taacattatc atcgttgacc gtctgggaac gagcgataaa
     4261 tggaaaaatc tggttaatct tcgcttcgtc gattatattc acaaagatga ttttctggaa
     4321 atggtcgata ccgatatgat gcctttcgac gtcgacgcga tcatccatat gggcgcctgt
     4381 tcctccacga ccgaacgcga tgccgattac ctatggcaca acaattacgc gtataccaag
     4441 attctggccg aatgggcgct ggagcacgat attcgtttta tctatgccag ttccgcggca
     4501 acctatggcg acggcgcaaa aggatttgca gacagccaca aaaccatccc cggactcaaa
     4561 cccatcaata tgtacggtta ttccaaacag gtattcgatc tgtgggtact gcgccagaag
     4621 ctcgaaagca gagtggctgg aatcaaattt ttcaatgtct tcggccccaa cgaataccac
     4681 aaaggcgata tgtccagcgt cattttcaag gcctttcatc agatcaaaga aaccggcaag
     4741 gtcaaacttt tcaaatcgta taaaaaagaa tacaaagacg gcgggcagat gcgcgatttc
     4801 gtttatgtca aagactgcgt caacgtcatg tggtggcttc tccaaaacag gaaggtcaac
     4861 ggcattttca acctgggaac gggaaaagcg agaagctgga atgatttaat taaagctgtt
     4921 tttacagcca tgaaaaagaa aacaaatatt gaatatattg ccatgcctga agctctgcgg
     4981 aatcagtatc agtattttac cgaagcggaa atgagcaaac tgaagaaggc gggttgtccg
     5041 tttaagtttt cctcgttgga agattccgtc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn caaactgaag aaggcgggtt gtccgtttaa gttttcctcg ttggaagatt
     5221 ccgtctatga ttatgtaacg aaatatttgc agaaatccga tccgcatttg ggaaaataaa
     5281 ttattcaact tacgtcactt cggcgaaggc cggagtctag acattatttt cgcaaaagcg
     5341 gaacaatttt atacactgga ttccggcctc cgccggaatg actaatgagg ctttttacgg
     5401 agttgtttat gaaaagagaa gttgtttgca tcatcccctc gcgctatgaa tccagccggt
     5461 ttcccggcaa gccgctggct gatttatgcg gcaagccgat gattcagcac gtctatgaac
     5521 gcgtggccaa agccaaagcc gttccctatg tggcggtagc caccgacgac cagcggatat
     5581 tcgatgcggt aaaacatttc ggcggcaacg tcgtgatgac agcaggaacg caccgttccg
     5641 gaactgaccg catcgccgaa gcggtcaagt cgcttaatct tcccgccgac gcgattgtcg
     5701 tcaacattca gggcgatcag ccgatattcg aaccgacgca ggtggacgaa gtgattcagc
     5761 ccctgctgga cgacccgtcc atcaacatgt ccacgctgat ttataaaatt gttctggaca
     5821 aggaaatcac ccatccgcac gccgttaagg tcgtcctcga tcatcagaat ttcgcgctgt
     5881 atttctcgcg ggcgacgatt ccgtatgtcc gggataaaaa tctgagagcc gattattaca
     5941 aacatcacgg catttacgcc tatcgccggg attttctcga tgtgttcacc aaacttcccg
     6001 aaggcaaact ggaaaaactc gaagcgcttg aacagttgcg agcgctcgaa tacggttaca
     6061 aaatcaaagt agtcgtcacg ccctatgatt ccgtggaagt ggacaatcaa caggaactgg
     6121 aaagagtacg cgagatactc ctggagaaaa aatgaacctt aaaataatca aaaaaacgtg
     6181 tctgctctta tggatgtttc ttctgtttac ggttgcggcc gcaatgccgg ccggcgccgc
     6241 cgacatggaa aacgctctga tggaatgcgc taaaatcagg aacaacgcca cccgactgaa
     6301 atgttttgat aaactggccc gtaagccatc catcaaagaa gaatccaccg ccacaacaat
     6361 cgagaaaaaa gagtctgtcg cagtcaccgc cggagacatg gaaacaagcg aagccgccgc
     6421 aaagcaaacg cccttatccg ttatggaaaa atactgggat atgagccttg atccccgtaa
     6481 ggaaagaaat atttttgtcc tctggccgta ccggccctgc tattttctgc cgctggcgta
     6541 taatttttca cccaacaagg atgccgcgct ggatttcgat cccgacgcca aggcaaaaca
     6601 caatgaagta aaatttcaac tgagcttcaa gtttaaactc tggcgagata ttatcaggga
     6661 tcataccata aaaaatatta tcgaaaaaag taccggcatc cgaggggttg atctctgggt
     6721 cgcctataca cagcagtcgt tctggcaatt atataacgat gccttttccg ctccctttcg
     6781 cgataccaac tatgaaccgg aactgctatt gaatttccgc atgcagcggg agatccccta
     6841 tcttttggga acaaagctgc agtttgtgaa catcggcttt aatcatcagt ccaacggccg
     6901 ctccgagccg ctctcccgga gctggaaccg tattgtagcc aatgtcggcc tggaaaaaaa
     6961 ttttggaacg gctaccaatg attccttcga actgctgctc aagacgtggt accggcttcc
     7021 cgaagacgat gccgacgatg acaatcccga tctttccaga tacatgggat acggcgagtt
     7081 atggggaacc ctctactgga aaaagcagcg gttcgcattg atgctgcgca ataatttacg
     7141 ttcggataac aaaggcgcgg tacaactgga atggagcatg ccgatggctt ctatcaacga
     7201 aaatctggcc aaaaagatca gcctctatgt gcagtatttc aacggatacg gagaaagcct
     7261 gctcgactat aataaaaacg tcaatcgaat cagcgccgga ttgatgcttg cggactggta
     7321 ctgatttttg gatgcaaacg tctaaaacac aaccggttta tctttatgaa agaaatcata
     7381 acagccacgg atgaccatac agttgaaagc tctctgggct ggcgggtcga tattttatcc
     7441 atggatgctt tacgatatca ggagagggac aaaaccatca catttgaaat cgaagactat
     7501 tcggacgcta ttggagaact cgagtggaca atttacatcc cgccgatatg caaatggcag
     7561 gatgaaaacc atccggaaga aatcattggt caggaaaaac tggacgacat tatcgacaga
     7621 atcagcacgg cattctggaa actcgacatg aaaattagag gaatagcatg atcacatctt
     7681 ctgtgaaaag aatttttctg ctgtggtctg atagttaagg ttatgaatta atgacgaaat
     7741 tacaggattg cttaaaaacg ccttttgttg tatgttttca taaaggattt tttgaggaaa
     7801 attgatgtca aatcatcact gaaacaaatc cgtataaatc gatcaaacac aaatactatc
     7861 aattttcttt ttagggaagt gctccatgag ttcagatttt tctcttgaat ttaaagaaag
     7921 gtacattcat ctaattcatg caccggatta tgaaatttcg ccggaaagca atgacatcct
     7981 atgggctgaa ttagccaagg cctgccgcaa atataaatgc ctgaaagtct taacggaagg
     8041 ctccaaccag aaaagaagaa ttacttccat ggacgcttat caatccggcg ctcaagcggc
     8101 ggagtccatt ccgggaatga ctctggccat ctgtattgac gattacactc ctgacgacat
     8161 tactcaattt tttaaaaatg ttgcctataa cagaggcgcc cgcatcgaat tcttcaacga
     8221 caagaaaaaa gctctcgcct ggctcggctt cgaagacacc gagtaaatcc caacaaggat
     8281 tttatataaa gtaaagttat taattattgt gggcctgaga atatatcatg actattcata
     8341 gctattcaaa attagaaatt gctactcagc aattggatac agcgttaatg ctttatttcg
     8401 aagacaaaaa ttatttctca gtcataactt tagcaggagc agcggaagaa atactcgcgg
     8461 tgtacctcaa gcttcataaa caacctaatg catttgagga aatgttggat tcatcgctat
     8521 taattggcga attattgtat aattcaaaag gttcaagaga cagtatgtac aaaacaatca
     8581 atcgagttaa aaatggttca aaacacatga aaggaagaga tgatataaat attcaatgta
     8641 atctaaaaga agaagcgcaa aacattcttg atcgtgcagt ttctaatttt tacacattga
     8701 tgacttttga gaatcttaat gaaacatccc tcatgaaaaa atttaattcc cagttagtta
     8761 gtaagtaata acaaataatc atctcaaaat gtttaaagaa cgtactttaa taaagaaact
     8821 cgaatgagct acaaatataa attgccgggt aatcaggcat tcgcctgact tccaacccag
     8881 caataacata ccttttgtat tccgcttcca aatcaaagat catatcaagg cggcaaggag
     8941 tcggctccac agacgtatat gataatacgt cgaggaagac gacgctgcca acaaagttag
     9001 ccgaagaaat caaaattggt atcagtcttt gaagatggcg cccgtcgagg acgacgtcac
     9061 cattttcgca tacctcgcca gataacccgt cgtaattctc ggcttgaatg gtttcaatgc
     9121 ttttctgcgt ttggccagtt cctgagcacc gactttcagg ttcagtttct tggccggtat
     9181 atcgatggcg atgatgtcgc cttctttcac cagcgcgatc ggcccgcctt ctgctgcttc
     9241 tggtgagata tggccgatgg ctgcgccctg tgtaccgcca ctgaaacgtc cgtcggttaa
     9301 gagagcaacc gttttatcca gacccatgcc aacgattgcc gaagtgggag aaagcatttc
     9361 cctcatgccg ggaccgcctt tgggaccttc gtaacgaatg acaacgatat cgccgccttt
     9421 gatttttccg ccaagaatcg ccttaatggc gtcgtcttcg cagtcaaaca cacgcgcttt
     9481 gccttcgttt ttctgcatgg ccggcgcgac cgccgattgt ttcactaccg ctccgtccgg
     9541 cgcgagattg ccgcgcagaa tcgcgatacc gccctgctga gaataagcat tcttgagagt
     9601 tctgatgaca tccgtattca acacacgggc atctttcaaa ttcccgccga cggttttgcc
     9661 ggttaccgtg atggcttttt catcaatgac acccagcgcg ctgatgactt tcatcacagc
     9721 ctgtatgccg cccgccgcgt ccagatcttc gatgtgatga cgcccggcgg ggctcaattt
     9781 gcagagattg ggagtttttt cactgatcct gttgaaaaga tccagatcga gtttgatgcc
     9841 cgcttcatgg gcaacggccg gaatgtgcaa aaccgtgttg gtggaacagc ccagcgccat
     9901 gtcgacagcg atagcatttt taaatgcagc caatgtggca atgttgcgcg gccggatatt
     9961 tttcttgagc aaatccatga ttttcattcc ggcgcgtttg gccaggcggt gacgagcggc
    10021 caggacggcg ggaatcgttc cgttgccggg caagccgaga cccagcgctt cggtgacgca
    10081 gttcatggaa ttggccgtgt acatgccgga acatgaaccg cagccggggc aggcacagtc
    10141 ttccagttgc ttcagttcag cggcggtgat tttttttgcc ctgacggcgc ccacgccttc
    10201 aaaaacactg atcaggtcaa cggtcttgtc ctgaaatttt cccgccagca tcggaccgcc
    10261 gctgataaaa atggacggaa tgttcagacg cagagcggcc atgagcatgc ccggaataat
    10321 cttgtcgcag ttgggaatga aaaccagcgc gtcaaaagga tgagccatcg ccatgatctc
    10381 gatggaatcg gcgatcagtt cgcgactggc cagcgaaaat ttcatacctt cgtgccccat
    10441 ggcgatgccg tcgcagacgc caatgaccgg gaactccacc ggtgttccgc cggccatccg
    10501 gattcccgcc ttgacatcct gcgtaatcaa atccagatgg atatgtccgg gaatgatttc
    10561 attggccgaa ttgaccacgc cgatcaacgg cctggccagc tcttcatctg tataccccat
    10621 tgccttaaac aacgagcgat gcggcgcccg ctcgaaacct tttttcatga gatcgcttcg
    10681 catggttatc tctcccttga taaaataaat ttctaataga aacttccccg cccctctccc
    10741 ctcaagggag agggcgtaaa atgaactttt taaggagtcg tctatttctt ctttcgtttt
    10801 tccggtcgta attcacgcac ggcagcgccg gctttccaca tttccgaatc acgcatctgt
    10861 ttaagttctt tttccagttt gacccggtaa tcgggagcac tgttggcctt caggacaatg
    10921 gctgtttcct tgccactcgc caccgactta taaagctcgg caaacacggg tgcaaccgct
    10981 ttgcggaatt tatgacgcca atccagagcg ccgcgctgag ccgtggtgga gcaattggca
    11041 tacatccagt ccataccgtt ttctgcaacc agacggatca ggctctgtgt cagttcttcc
    11101 accgtttcgt taaaagcttc actgggcgta tggcggtgtt tgcgcagttc gttgtactgg
    11161 gcttccatga tgcccgccag cgcacccata agcacgccgc gttcaccggt caaatcgctg
    11221 taaacctcgt tctggaaggt tgtcgggaat aaaaatccgg agccgatggc gatgcccagg
    11281 gccagcgttc tttccattgc gcgtcccgta taatcctgct gcacggcaaa gctggagttg
    11341 ataccgctgc cgtccaggaa attccggcgg acggatgttc ccgacccttt gggagctacc
    11401 atgatcacat caacaaattt tggcggtata acgccggtct gctttctgta aacaatggaa
    11461 aatccgtgtg agaaatatag agcgtcgccc tctttcaggt tagccttgac cttgggccaa
    11521 agaagtttct gcgccgcatc agaaaccaga tactgaatga tggtaccgcg cttgaccgct
    11581 tcttcaatcg ggaaaagagt cttgccgggc ttccatccat cggcaatggc ctttttccag
    11641 tagcgcgtat cttccttggc ctggccgatg atcacattaa tgccgttatc cttcatatta
    11701 gccgcctgag ccggcccctg cacgccgtac cccagcactg ccaccacatc attcttcaaa
    11761 actttctgag cttttttcag aggaaattcc ttcgccgtaa taacatcttc gagaacaccg
    11821 ccaaaattaa tttttgccat attaaccccc tattgaataa aatattataa tttagttttc
    11881 caaatttaaa gcgacaatgc cggttcttgt gatatcggct atgcccagcg gctccagtct
    11941 ggccagcgca aaatcaatct gtgaccgatc accggttatt tccagaatca cgtaggtgtc
    12001 ggcattgact attaccttcc atttattcat gtcaacagct ttttttatct gttctttttt
    12061 atcggccgcc agatttaacc gcacgaggat caattcgcgg tgaatcgatt ccacattggt
    12121 caaatcatcc acctgaatta catctaccag ccttcgcaat tgcccttcga ttctgttgat
    12181 ggtgtcctgc gtacccacgg tggtcatcac gatttttgat acttcgtaat gtgtggttga
    12241 attcacgcac aggctttcga tgttataacc cttaccgccc aggatgccgg cgattctcgc
    12301 cagcacgtcc ggtttgttgt gaaccagtat tgaaatgata tgttccttct tttccatctt
    12361 ctccctcatc atattattta ttatttaata cttcttattt aaaaactttg tcaaaagcta
    12421 cagaggcaag gcgcgcgaag cctgagaagt gaggcgtatt tttcacatac gccgcaacgc
    12481 cgaaggatga agcgcaacgc agcatctgga cttttcacga aatcgtcaat tgatcatttc
    12541 tctgctcccc agcgacatgt tgctgatcga atcgcccgga tgcaccatgg ggtaaacgca
    12601 ttcttcccgg gagacacgaa aatccatcag caccggtttg tcaatagcca gtccttcttt
    12661 taacacaggc tccacgtctt ccggttttgt ggcgcgcaag cccacgacgc cgaacgcctc
    12721 agccagtttt acaaaatcag gcgcataggt catatctgta aatgaatacc gtttttcgta
    12781 aaacaattcc tgccactgac gcaccatgcc cagatagccg ttattcagca gaacgatttt
    12841 tacattgatt ttatactgcg cggcggtggc cagttcctgg atattcatct ggatactgcc
    12901 gtcgccggca atgtccacca cgtgcttgtc gggaaaagcg catttcacac cgatagccgc
    12961 ggggaaaccg aatcccattg ttcccagtcc gccggatgaa ataaacgtat tgggcttgtc
    13021 aaacgggaag aactgcgcgg tccacatctg gttctggccg acttccgttg taataatcgc
    13081 gtcgccgccg gtcaacttat acagtgtctc gatgacagac tgcggtttga tgacgttgcc
    13141 gttctggcaa taggtcagcg gcactttttc cttccagccg gcaatttcat tcagccattc
    13201 atttcttgca tccttgtcat aagcgtaatt gtgttcgtcc agaaatgtga tgaggtcctt
    13261 cagcaccgcc tgggtatcgc cgatgatcgg cagatccacg gcaacgtttt tattgatgga
    13321 tgacggatca atgtcaatct gaactatttt ggccttggcg gcaaacgtat cgatggtgcc
    13381 ggttacccgg tcgtcaaatc tgacgcccac ggcaatgagc agatcacaat ggctgatcgc
    13441 catgttggca tagtacgttc cgtgcatgcc cagcatgccc agccaaagcg gatcagtccc
    13501 cgggaaagaa ccaagcccca tcagggttcc ggtcaccggc atctgatatt tcctggcaaa
    13561 ctccgttagg agcgatgacg ccctgcccag aatgacaccg ccgccggtca taatcagagg
    13621 ctttttcgcc gctgtcagca aatccagtaa accggccatc tttttcggag ccggtttgta
    13681 cgcggcctca tgatttctga ttttgatgtt ggccggatca tattccgtct gggccgccat
    13741 cacattttta ggcagatcaa caagcaccgg accgggtctt cccgagcggg caatatcaaa
    13801 cgcttccttg atcgtcgcag cgagatcgtc aatattgcgc accagaaaat tatgtttggt
    13861 gcacggacgg gttatgccgg tgatatcgca ttcctgaaaa gcgtcgttgc cgatcagcgc
    13921 agtgggaacc tgccccgtaa aaacaaccag aggaatggaa tccatattcg ccgtggcaat
    13981 gccggtgact gtgttggttg cgccgggacc cgacgtcacc agacaaacgc cgacttttcc
    14041 ggtggaccgg gcatagccgt cggctgcgtg cacggctccc tgctcatgcc ggaccagaat
    14101 gtgtttcatg ttgcttttgt atatctgatc gtaaatatcg agaacagcgc ctccgggata
    14161 cccgaagata atttcaacct tctcctcttc gagcgctttc agcaatattt ctgtcccctt
    14221 cagtttcatt ccttttcccc tttaaaatga tttataatta attttttacg gtcttgtttc
    14281 aggccatgtc tctttcgctt ttttaaagat cacttaaccc gcttaccctg cccagaatta
    14341 atccgccgta caaatttcca cctatagcga ccttcaggat atgcgttcct gaaagttgag
    14401 tctccgatcc cactccgctt cgctccgggg attgttcgtt ctgcaaattt cagcgtgctc
    14461 cgcttctgaa atttggaact cacaaaaaaa agccgcctgt ttccttgaga aaaagcggct
    14521 ttatgccaaa aacaacaaag ccgcgggctt caggggcctg cggctagtat cttttattcc
    14581 ggtttcttaa agatacagag cagcagaccc cgtaagtacg actactacga ggaccagtac
    14641 tttattcagt acccggataa tgccgctttg taaaacttta agaaacataa acacatcatt
    14701 ccgttgaatt atttcgtgct ttcaataacg aaaaacactg tctttgtcaa caatattttt
    14761 tgccggacat tctttccgga tcgtcgacac gccccaggaa taactcccgg ctaccggaat
    14821 aaacctttaa tagaatcgcc cagagtgctg ctgggagacg ttcccaacag gaaggcttca
    14881 ttggtcccct tttccgaagg tccggcaggc tctcccgttt tcggattgat tttttcaaag
    14941 acgatgcctt ccggaacggg aaaagactcc accggtgtgt tctgcaaggc cttttccata
    15001 aaataaagcc agatcggtgc cgccgcccgt ccgccgactt cctgtgatcc cagcgaggct
    15061 tcctgatcga aaccgatcca cactccggtg atcagcgaag gcgtcatccc gataaaccag
    15121 gcatcacgcg tatcgttggt cgttccggtt tttgcagcca ccggacgccc tatgctcctg
    15181 actctcgtac cggtgccgct tgttacgaca tcctgcatga catgagtcgt gatgaatgcg
    15241 atgcggggat caatcacctg ttccggctga acttttgttt cttcaaaaac attgccggtg
    15301 cgatcaacga tttttttgat gaaaaaagga gaaactttct gtccctgatt agccaaaacg
    15361 ccatacgctt gaaccagctc ctgcagcgtt accccggatg cgcccagtgc caaagacagt
    15421 gttcgcacca gaggggatgt aattcccata ttcatggcgt acgaaacagc ataatcaatg
    15481 ccgatttcct gcaaaatttt taccgttaca acattgcggg acagcacgag cgccgttctc
    15541 attgtgatcg gccccatgaa tttgtcgtca aagtttttcg gcctccagat gccgtcaggc
    15601 tgcgaaacat catccacagc aagcggtgaa tcgtcaataa cggttgcagg cgtcatgcct
    15661 ttatcaaaag ccgccgtata aatgatcggt ttgaacgctg aaccggcctg acgtcgtgac
    15721 tgcgtcgcgc ggttgaactc gctttgattg aagtcgcgcc cgccgaccat cgcacggatg
    15781 gcacccgttc tgacgtccat gcagaaaagc gcgccttgca cgagaccgga tttgtattta
    15841 ttccgttcct ccagttcctt caagccccgc tccatcgcct ccccggcgta cttctgcgcg
    15901 gtcagatcca gcgtggtata gatggaaaga ccttccttgt acaaaacatc ggcgccgtat
    15961 ttttcctgaa cataccgtcg aacggtctcg acgaaatagg cggccatctt gtccttgggg
    16021 cggatggatc gcagattcat cggcgcagca tacgcttttt ccatttcctc ctgggaaata
    16081 tagccgtctt ctttcatgcg cgtcagaacg taatattgtc tttgtttggc tttgtcataa
    16141 tgaaggaaag gggaataatt gctgggagct ttgggcatac cggccagaag agccgcttcg
    16201 tgcaatttca gttcccgggc actcttcccg aaataaccaa gcgaagcgga ctctatgccg
    16261 taagtaccat ggcccagata aatctggttt aaatataaat tcagaatctc gtctttggtc
    16321 aggtaccgat caattttgta agctagaata gcttccttga atttacgggt atatttcttt
    16381 tcgggagaaa gatacatcat cttggcaacc tgctgggtaa tcgtgctgcc gccctggatg
    16441 atatgtcccg cctcgatgtt tttgaaaacc gcccggaaaa tgctttgcag atcgaaaccc
    16501 ttgtgcgcaa agaaacggga atcctccgcc gccacaaaag cctgaacaac aactcgcgga
    16561 acttccttaa tcttgatgag ttttctgtct tccagaaaaa actcatcgat cagttcgttt
    16621 ttatcgtcat aaacgcggga agaaatgctg ggacgataat ccttgagcgc atcgataccg
    16681 ggcaaatcca tcgtaaaagc atagaatccg atgaccccga aaactgtcat caccagaatg
    16741 aagacgacaa agcccagaaa aaaattaaaa agcgaagaaa gttttttagc ttctccgtcg
    16801 ttttttctgt ttgacttttt acgatttaga aacattttac agattatcct tcgtcatttt
    16861 gggattcttc ttttttcgtc tcggcaaatt tggaaacaaa tatgcttatt tcatacagga
    16921 tcatgagagg aattgccatt aatatctggc tcagggcgtc cggcgaaggc gtcaggactg
    16981 cggcaaccag aaagataaca agaatcgcgt atcgcctctg tctggaaagc ttggctgcat
    17041 tgacgatccc cagtttggtc agaaaaaaaa tgaaaatcgg caattcaaaa gacaaaccga
    17101 acccgagcag gaacgttaca aaaagagaca aataatcctt aaacgaaagc atcgcctgca
    17161 agtattcatt attgaaactg acaaaaaatt tgaacgccgt gggcaaaacg ataaaatacc
    17221 caaagagaac gcctccgata aaaagcagag atgaagaaat gacaaacggg acaacatact
    17281 gcttttcttt cggaagcagt cccggtgaaa ggaatttcca tacctgatac agaatgtagg
    17341 gactggtaat gatcagggaa gcaaaaaaag cgattttcat gtacgtgaaa aaggcttccg
    17401 tcaaaccgat atagacaaga tgactgtctt tcgacaacac ttgagtcagc ggacgggtca
    17461 tcacatcaaa aatcaggtct ttaaaataat aacagacacc gaaaccggcc gccagcgcaa
    17521 ttaaagaacg ggtgagcctt ttgcgcagtt ccagcaaatg atcggtcaga gacatttttt
    17581 gatcttcggc ggcctgggta ttgtccatag tttgacgttt tgccttttgc agcgttgata
    17641 gtcagagcga atcagtttat cgtttggatt tattcagatg aatcggattt tacgttgtca
    17701 tgatcgggct tcttcattaa aagagaattc ttccattcgt cgtcattctt cggtttttca
    17761 tccactttga cggcgtcctt gatatcttca gtcactccgt cagccgctct gcggaattcg
    17821 ctgaagcctt taccaagaga cttggccaga tcgggcagtt tcttaggacc gacaaccaga
    17881 agagcaatta tcgcaataat tatcagttcc tgcatcccta tgccaaacat cgtgtatcct
    17941 ctccgggcaa cttttgtatt cctatatatt gattgctgtt tttgtcaatg ttttttgtcg
    18001 cagacttttt tgaattttcc gaattcatca aacagcgatc aaacgaacaa aaacgtccat
    18061 tttatctttt cctgttttaa agcggtgatt tcttttgctc tttgcaacta tttatggtaa
    18121 acggaattaa ctttttttaa cggcattttt accgaagaag gttcacttgt ccaggaaaac
    18181 aggcgtcgtc aaagatggaa aatacatgca gcattcgatg ggttttgctc atcccgaaag
    18241 cccggagagg ctcgccgtca tttacgaaat gctggaaaac ccgctgatgt cctggaaatt
    18301 cacgccgatc gaaccgcgtg aagccactca cgaagaaatt gaaaggattc atgctcgctc
    18361 ttatgtggac tttatcgcca gcaccgcggg caagcgcagc gtttttctcg atcccgacac
    18421 atcgacatct ccggaaactt acgacgccgc aaagctagcc gccggcggtg tctgcaatgc
    18481 cattgacagc gtcatgaaag gcgacgtgga taacgctttt gcttttgtca gaccgccggg
    18541 acaccatgcg gaaaaagata cggccgccgg cttctgcgtt ttcaataata tcgccatcgg
    18601 ggccatgcat gccatccaaa aacacaattt aaagcgaatt ctgattgtcg actgggatct
    18661 gcatcacggt aacggcaccc agcacagttt ttacggggat cagcgcgttt tatatttttc
    18721 cacgcatcaa tatccctatt atccgggaac gggaagcatt tcggaaaccg gccggggcca
    18781 gggcgaatat tatacgatca atgtccccct gaccatgtct gtcggcgatg cggtctttgt
    18841 caagatattc cggaagattc ttgaacccgt ggcgctggaa ttcaaacctg aattgatcct
    18901 gctgtccgcc ggttttgata cgtattatca ggatccgctg ggcgcaatgc aggtcacgcc
    18961 ggagggctac gccgctatga cgcgtatcct tctcaatatt gccgacacct gctgtcaggg
    19021 acgttttgta gccgtcctgg aaggaggata tcatctgagc ggactggtca agtcggtaaa
    19081 agccgtcttg aacgaaatgc tggatgaaac ccatttctcg gaagaacgct tgtctctgat
    19141 ggagaaagac gtggatgaaa gaacaacgga atccgtgatc gataaggtga tcaccagaat
    19201 aaatccctac tggaaagttt tttaaaggag atgatgtttt ttgaaaataa gttcccgcga
    19261 agttgagtat gtggctcatt tggcgcgtct ggaaataacg gacgcagaaa aagagaaatt
    19321 tacggctcag ttaaacgaca ttctgctcta tattgataag ttgaacgaac tggataccgg
    19381 ggatgtgaaa ccgataagcc atgcaatcag cataaccaat gcgtttcgcg aagataagat
    19441 atcaaactcc atcggcaccc gggaggcatt ggccaatgcg ccggacaccc gaggtgagtt
    19501 cttccgcgtc cccaaagtaa tcgattaaaa tctcgtccga aatcacaacg ttgactattc
    19561 caggccctaa ttaaatataa aggaatttta tgaagttgaa tcagttaacc attcatgaac
    19621 tgcagaaaag aatcaaaacc ggcgaaacat cggccacgca aattactcat gatgtttttg
    19681 ccagaatcaa tgctgtcgac gaaaaggttc atgcctatat tcgtctgatg caggaagagg
    19741 cttttcaggc cgcgtcaaaa gcggatgaag acattaaaca aggtgacata agagctttaa
    19801 ccggcattcc tatcgctctg aaagacattg tctgcaccaa aggcattccc accacctgcg
    19861 gatcgcgcat tctgcataat tttattccgc cgtatgatgc aacggtcgtg gaaagactgc
    19921 gcaaggctgg agcgatattt gtcggcaagg ccaatatgga tgaattcgca atgggttcgt
    19981 ccacggaaac ttcctatttc ggtgccacac gcaatccatg ggatctggaa cggattcccg
    20041 ggggctccag cggcgggtcc gccgccgcgg ttgccgccga tgaatgcatc gcctccatcg
    20101 gttccgatac cggcggctcc atccgtcaac cggcggcgct ttgcggtact gtcggcatga
    20161 aaccgaccta cggacgggtg tcgcgcttcg gtctgattgc ttttgcctca tcgcttgatc
    20221 agatcggacc gtttaccaaa gatgtcgagg actgcgccat catgatgaat gtgctggccg
    20281 ggtatgatcc gaaagaatcc acttccgtga acgaggaagt tcccgattat cggcaatttg
    20341 tcgggcgcga catcaaaggc tggaaaatcg gcatcccgaa ggaatatttt atcgacggca
    20401 ttgatccggg agtccatgcc gccattaaga aagcgattac cgtcattgaa cagagcggtg
    20461 gccagtgcgt ggatatttca ctgcctcata cacaatactg tgtggcaacc tattatatta
    20521 ttgctccggc ggaagccagt tccaatctgg cgcgctatga cggcgtccgg tacgggcttc
    20581 gggcacaaga tgtccgggat cttttgatga tgtataaaga atcccgcaag cagggtttcg
    20641 gcgcggaagt aaaaagaaga attatgatcg gcacctatgc cttgtccgcg ggatattatg
    20701 acgcctatta caaaaaagct tcgcaggtga gggctctgat caagaaggat ttcgacaaag
    20761 cgtttaaaat ttgcgacgtg attatgacgc ctacatcccc gacaccggct tttaaaatcg
    20821 gcgaaaaaac agatgatccg ctgcaaatgt atctttccga tattttcacc atctcggcca
    20881 acctggcggg aataccggga atctcggttc cctgcggatt tagttcgagc ggtctgccga
    20941 tcggagtgca gtttctggcg agccattttg aagaaggcaa actcattcaa atcgcctctg
    21001 cttacgagaa aaacgccaag attgaaaaga ggagacctgc attataatgg attttgaaac
    21061 agtcatcggg ctggaagttc acgcccaaat gttaacggac acgaaaattt tctgtaactg
    21121 ctccaccaaa ttcggcgctg cacccaacag ccacacctgc ccggtttgtc tgggcatgcc
    21181 cggcgttttg ccggtactga ataaaaaagt cgtggaatac gccctgaaga tggcactggc
    21241 aaccaactgc gaaatcaata aatcatgcga atttgcgcgc aagaattact tctaccccga
    21301 tctgcccaag gggtaccaga tatcccagta tgcctatccg ctggccgaac acggctatgt
    21361 catgctggag acgaatggcg agcagaaaaa aatcggcatc acccggattc acatggaaga
    21421 agacgccggc aaactgctgc atgacgagca caacccggtc agttacgtcg atttaaaccg
    21481 taccggcgtt ccgctgattg aaattgtcag cgaaccggat atgcgttcgc cggaggaagc
    21541 agccgattat ctgaaacgcc tgcacgaaat tctagtgtat ctggaaattt gcgacggcaa
    21601 catggaagaa ggttccttcc gctgtgatgc caacgtatcg attcgtccca aaggccagaa
    21661 ggcattcggc acccggaccg aattgaaaaa catgaattcc ttccgcaatg tgcagcgcgc
    21721 gctggaatac gaaatcaagc gccagcaata cctggtggaa aacggcggca cagtcattca
    21781 ggaaacccgg ctttgggatg acgcccaggg cgccaccaat tcgatgcgca gcaaggaaga
    21841 agcgcacgat taccgttact tccccgatcc cgatcttgtc cccatcctgg ttgatgaagc
    21901 gtgggtggaa aaaatcagaa aagagcttcc cgaactgccg ctggccaaac gggaaagatt
    21961 catcaaggat tatcaaatac cggcttacga tgccggcgtg ctgaccgccg acaaggcgct
    22021 ggctctttat tacgaaaagg tggtcaaact ctgcaacaaa cccaaaatcg ccagcaactg
    22081 gctcatggga gacgttatgc gttttctcaa tgaagataaa cgctctgtcc gcgaatgtcc
    22141 catcaaggcc gaatcgctgg ccgatatgat tcgattgatt gaagaaggcg ccatcagcgg
    22201 aaagatggcc aaggaagttg tggaggatat gtataagacc ggcaaatcgc cgcagaatat
    22261 catcaaggaa aagggcctgg tgcagattac cgatagagat gctctggtta aaacagccag
    22321 cataaccgtc ggtaaatatc cgcagcagcg tgatgattat cgcggtggag gcagggaaaa
    22381 actttttggt ttttttgtcg gtcaggttat gaaagagacc aagggcaaag ccaatccgca
    22441 gcttgtcaat gaaatcctga aagaaattct tgataagaaa gatgattaaa acaattttct
    22501 ggaaaaacaa tgcggtcgtt ttaatcgacc agaaagctct gccgcttgcc gagaaatata
    22561 tagtctgcaa atcatacaaa gcagtgattg cctgtattaa agatttgact gtgcgcggag
    22621 ctcccgccat cggcgttgcc gctgcgatgg gcgccgctct gggagcgatt catcttgcct
    22681 ctttatcgcc ggataaattc cggaaaaagt tttacgccat ttgtgatgag attgcccagg
    22741 cacgccccac ggcacgcaat cttttctggg cgctggagcg catgaaaaaa attgtcagtc
    22801 attcaagtca actatcccaa cgagatatag tcaaagaact aatcaaaaaa gccaagcata
    22861 tctgctccga agatatagcc atcaatcaac aaatggggaa aaacggaagc cggataattg
    22921 ccgacggcga caatattctg acgcactgca atgccggcgc gctggcgaca gccggatacg
    22981 gaacggcgct gggcgtcatt cgtgcagccc gtaaacaagg caaaaaactg cacgtttacg
    23041 tggacgagac cagacctgtc ctgcagggcg cacggcttac cgcctgggaa ctcaaaaaag
    23101 aaaaaatccc ttacacacta atcaccgata atatggcggg atttctcatg cagcagaaaa
    23161 aaatagacaa gattatcgtc ggggcagaca gaattgcggc caacggcgac acggccaata
    23221 aaatcggcac ctattctctg gcggtcctgg cgcaggctca cggcgttcct ctctacattg
    23281 cggcgccgct ttccacgatt gacgtttcat taaaaacagg agactcgatt cccatcgaag
    23341 aaaggaaaag cgatgaggtg gcacatcttc aaggaacccg cagcgctccc aggggaacga
    23401 tcgtttataa tccggcattt gacatcacgc ccgcccggct gatcacggcg atgatcacag
    23461 agaaaggaat tttaagaaaa cctttccgcg catccattgc ccgtctgtat aaaggagaaa
    23521 ggggatcatg aaattattcc tttttgattt tgacggcgtg cttgtcgact cgcttgacgt
    23581 ttatgaaaaa acggtcaagg acaaccttca aaaaattaaa caacctgttg tacaaagccg
    23641 ggaggaattt ctggaactct ttgaagacaa tttctatacc tcgctgaaaa acaagggcat
    23701 tgatctgaat gtgtttatga aagccgccga ggatattctc gcgcaggtcc ggatcacgga
    23761 catgaaaccg tttccggatg ttgcgccggt tatcgaaacg ctgcacaaga accactgcct
    23821 gattgttgtt tcctccaatg attatgcctc catcaaagag gcccttgatt tatttcattt
    23881 caatgattat ttccgggaca tactcggttc cgatttcatg ttcagcaaaa aagaaaagat
    23941 tctttatgcc gcggaaaaat atcatgtgga acccaataaa atatattata ttggcgatac
    24001 gaccggcgat atcaaggaag gaaagcaggc cggggtaaaa accgtgggag tgacctgggg
    24061 atggcacagc agagaaagaa tggctgctgc cgaaccggat tacctgttcg atacgcccgg
    24121 cgaacttttg caactaagct aggtttttaa acatgaatga tttactggca tatatcagca
    24181 actggtctgt tctgaaatgg gttgtgcttg tgctgattgc cggatttatc ggacaattcg
    24241 gcagaatggc ggctgaagcc atcgccaaaa aaatccgtgc aggccggccc caacaggaaa
    24301 acaaaacaat aaagccggaa actcccttgc agactccggc accgcagcct gtatcatccg
    24361 gtgaccttcc cgacaaaaaa ctcctcaaag cgattgccaa agcgaaaaaa aaggaggcga
    24421 aaaaaaaaga gtaacctatt ccgacaggcg cctgatgtgt gtttccagca gacgattctc
    24481 atcaaaatct ttcagtctga gatattctgg cgtcgcaccg gcaaaagcgc atttcggcgc
    24541 caatccgatt aactccgatt ccaaaatctg cgcaccgcct tcagccgcga gagctttcac
    24601 ccggtcaaag acggttttca agggtgtttc cttacaatcg gtcagattga ccgagacctg
    24661 cgccagtctt cgtcttttca gcatcactcc gatcgcccgc acattcttca atccaccgcc
    24721 cttctctctg atgcgcgagg cgatatgccg cgccagaagc agatcgtcag agaccagatt
    24781 gatattgtag gcaaccaata aatcccgcgc ccctacagcg gtggcgccgg accgcgcgtt
    24841 gaattgattt tctcccgcat cgggaaggct ttccgtagaa gacattttat cagccagtct
    24901 ttcataaccg ccgcggcgga catcgggaag ttcaacattc cgaccgttgc gcgcagcgta
    24961 accgtaaaaa tacaccggca cgccgaaccg ctgggaaaac cgcatgccga acgcatgagc
    25021 cagttcaacg gcatctttca tttttgcccg gcccagcgga ataaaaggaa cgacatcgac
    25081 ggcacccagc cgcgggtgaa cgcccgactg ttgccgcata tcgattaatt ccagggcctt
    25141 tccacaggcg gccagtgtcg cgtccagcac atcatcgggt tttcccaaaa aagtaaaaac
    25201 acttctgtta tgatcggcgt ccccgctgaa atcggctaaa cggacgccgg gaaacatttt
    25261 aaaaacagaa acaatggata agattttttt gtcatcgcgt ccttcactga agttcggtac
    25321 gcattcaatg atcttcatgt ctatgcctga taaattttta agcccggaaa ttcgatacag
    25381 gtcagtttcc cgccgtaaac tgcgccggta tcaatgccga tcttgttttt gtcaatcagg
    25441 ggggtcatta acggcgtgtg accgaacaca accgtcttgt caaagtcttt ttctgtattg
    25501 ataaactcgt accggatcca taatagatcg ttttgcgact gttccgcgac cggaatccct
    25561 ggtctgagac cggcatgaac aaaaatatat ttttcggttt cataatacgg cagaagggtt
    25621 tcaaagaatt tccggtgcct tgcggacatt ttacgttttc tttccttgat atcatcggac
    25681 aaaaatattt cataggaacg cagggtctgc atgccgccgt ttctcagata aatctcttca
    25741 tccacgccct ccagataatt cataaacatt tgttcgtgat tgccgagcaa acaaactatg
    25801 ttttcatgcg aatcctgcag gccggaaaca taatcgacaa cgtccctgct gaattcaccg
    25861 cggtcgacgt aatcgccgat aaacaccagg gtgtcttttt tgaaatcgat gtcaatatcc
    25921 gatatcagcg ttttaagctt tgtcagacaa ccgtgaatat cgccgatggc atagattttc
    25981 cccatatgga tctgctcccg ttgcctcggc tcttaatcca gcagtgcttt ggtaaaattg
    26041 ctgctttcaa aaacgcgcag atcgtccacg ccttcaccaa caccgatata ccagacgggc
    26101 aatgcaagtt cactggcaat gcgcacaacg actccgcctt tggaggtgcc gtccagtttt
    26161 gtcagcacga tgcctgtcgc gccgatttct tctttaaacg ttttggcctg agccacggca
    26221 ttctggccgg tggtcgcatc cagaatcagc agaatctccg gcggcacatc cggcaattcc
    26281 ttgttgatga ttcttttcac ttttttcagt tcttccatca gattgacacg ggtgtgcagc
    26341 cgccccgcgg tatccacaat caccaccccg ttgaaaccgg ccttgacttt cgacatcgcg
    26401 tcaaaaacga ccgctgccgg gtcggcgttt gttttgtgtt taataacggg agcgcccacg
    26461 cgtgctcccc agacctcgag ttgttcgacg gcggcagccc gaaatgtatc ggcagccacc
    26521 agcaaaattt catggccttc acttttcagg atattggcca gctttccgat ggtcgtggtc
    26581 ttgccgctgc cgttaacgcc gatggtcata atcacaaacg gtttcccgcg gggtatgatc
    26641 aggggctttt ccattttttg cagaatggcc gtcatgcgtt cctgtaaaac ttttttgatc
    26701 tcctgagcgt tattcaattc attgcggctg acgcgctgtt tgacgtcatc aatcagatcg
    26761 taggtaaact gcggccccat atccgctgaa atcagaagtt cctccagatc ttcgaaaagt
    26821 tcctggtcca gaaccctctt gccgaacacc agattatcca gactcttggt cagggcggtt
    26881 ctggtcttga ccagccccgc tttcaatttt tcaaaaagac tcgatttagc catagaagca
    26941 cctttcatcc tgccacaatt gattcataac cgcttttttg taagaagtaa agagacggat
    27001 taattgagat tgacggaaac cagagtggat atcccctgtt tctgcatggt cacgccgaag
    27061 agcgtgtcgg caacttccat cgtgctcttg ttatgcgtaa tcatgacaac ctgggatttc
    27121 ccagcgacat ccttgatcag acgattgaac aaatttgtat tggcatcatc cagggcggca
    27181 tcgacttcat ccagcaccag aaacggcgag gggcgatatt tcaaaatggc aaaaataaga
    27241 gcaaccgcca ccagtgattt ttcaccgccg gaaagcagac tcacattctg cttgcgtttg
    27301 ccgggtacct gaatatcaat atcaacgcct gtttccagca tatcattttc gtccgtcaaa
    27361 agaagttctc cgcgtccgcc gggaaaaatc tgcgcgaaga cttctttgaa gcacgcgttg
    27421 acggcttcaa aggtttccgc aaatctggcg cgggaaattt tattaatccg ggcaatggtc
    27481 cgctgcaaaa cattgatgga tgcattcaga tcggcaatct gtgtcgacag aaaattgtaa
    27541 cgctggtcga gttcctcata ctcattcaag gcaaggagat tcacctcgcc gaatgtctcg
    27601 agcatctgtt tgtttttttc cagagcatca acgatctcga ccagtttttc ttccgtgacc
    27661 ttttcaaaat ccgcggccat gccggccaga tcaacatcat gtttttcggc cacgcctttt
    27721 ctgaggtttt ctctgtttag ggttatttcc cggctttgaa attccatcgc attggcctgc
    27781 tggcgtaatt catccagatt cttcttgatt tccctgattt caccttcccg gatctttagt
    27841 tgtgcatctt cacggttttg ctcggctttt ttctcggcca gcaatgcttc ctgtgccgca
    27901 aacgcgctgt atatttcttt taacaaaacc tgatcgttct caatgccttg cgtcaattcg
    27961 gcgatctgct tttcacagaa cacgatgtcc tcttctttgg tcctctgttc ctgttccatg
    28021 gaatcaatat gatcctgcag cctggatatc gtccgtacat ccccttcttt cttctcctcc
    28081 agagaggcca gttctatttt cccggcggtt acataacgtt cctgttcgtc aatctggacg
    28141 cgggactgtt ctttttgaac gtttaacgcg gcgatgatgt cgttgatctg tttttcttca
    28201 ccgtcctttt gcaaaacatc ctcattgaac cttcggagct tttcttccgc ttccgtcttt
    28261 tctgtattca gggtttgtct gttgaactcc agaacggcga ttctctgtct gagtctgttg
    28321 acatcgtcgt caaaacgttc gcgatccttt tttctgccgt tgacggtaat ttccagcgca
    28381 tgaatttccg tcttcatttg agagctttct tcttcccagc gggaaatggt cgctaagatt
    28441 ttagttctcc ggccaacctt ggtttccaga tcgagagata aggctgcaat ctctgcctcc
    28501 agttcgccga tttctctttt tgtcgccagc aggctttttt ccaccgccga accgctgccg
    28561 ccggttaaca caccgtgcgg gctgatgata tcgccctccg gcgtgacaaa cgtcccccgg
    28621 aaaccgtttt tcttccagag agagatgccg ttttcaatcg tgggaatcag cagaacgtca
    28681 cccagcaggc agtcgataat gggtttaaaa tcatcgcgga ctgttacctt gtccagcagg
    28741 ggtttcgctt cattgaggtg cgcttcgctg taagtcctgg catcatgggt tctcaaatcg
    28801 acggaaataa aactgccccg gcccaactga taattcttca agtaatcgat agcgcgcaca
    28861 ccgtcttcct gactttgcac taccacgtac tgcagttttt cacccaggac ggcttccacg
    28921 gcagcctcat attcacgggg aacactgata tgatcggcaa tcacgccgta aaatttatca
    28981 ctgtgctctt tattctcgat aatcgtttta acgccctcgt tactccattt ggatgactcc
    29041 tgaaactccc gcagagaaat cagtcgggtg gatttgacgc tggtgtcttc cctgagtctt
    29101 gcaatatctt cctcgttaaa ctgtaaatcc gatctgaatc gatccagttc ggttgccgct
    29161 tccgccattt tagcttccag tccgacaatg tccgctttct cttttttcaa ggcttcgtcg
    29221 gccgcctcca gcctggcgca tacgtcggcc atcatcttct gatcttcttc cagttcgcgg
    29281 gtttcccttt cctctcgttt cttcagatcc tcgatatttt tgctgaggct ggaaatcata
    29341 ttatgaagtt tggctttttc cgtcacgaca tcgataaaca aaatcttttt ttcttccagc
    29401 tgactgctca atgccttgtc ggcttcggtg agttcctgga ctttttgctg aagcgattcg
    29461 tgttcggctt tcaaagcgac gattttattt tccgcttctg ccgaattttt ccgcagattt
    29521 tcgatttcag cggctaaatc ggcttttctc gtctgcagca cctgaatttc cgtcaaatct
    29581 ttctcccggc gtccggacat atcggtcacc tgacgttggg caaactcaat acttttttcc
    29641 ttgatactga tggtgttttt gatttcgtaa agttcctgct gaactttcgc cgtcagttcc
    29701 tcattctcca gaagtttcac tttcatttca tccagcatcg attctttcgc ttggaaatcc
    29761 gcccggatga gagcttcctg ctgctgtaat tccgtgcgct gttgctgcag atccgactcc
    29821 ctggccataa agtcgacata ggtttgcagg gcaaccgtta tttccgcttc tcggatttcc
    29881 tgcttgattt ttttgtactg ttccgctttc ttcgcctgac gggaaatggt gttaagctgc
    29941 gttttgactt ccttgataat atcgttgagc cgcagaatgt tctgtttcgt caggtccatc
    30001 ttacggacgg ccgcttcctt gcggctttta tatttggaaa cgccggcggc gtcttcgatg
    30061 tacaaccttc tgtcttcagg cttagcctca accagattgg tgacatggcc ctgttcaacc
    30121 agcgaatatg tccgggcacc gacaccggtc cccatgaaga attccttgat gtcgagaaga
    30181 cggcagggaa ctttattaat aaaatattcg ctatcgccgt ccagaacaac tctgcgggag
    30241 atggagactt cgtttaaatc ggaataagca ccgggaaaac tctggccgtt ggcatacaac
    30301 gtcatcgtca cttcagccat gctgaccggt gccgaatcct ggctgccgtt gaagaccaca
    30361 tcatccattt ttttgcctcg cagtgccttg actctctgct cacccatcac ccagcgaatg
    30421 gcatcgacga tattagattt gccgcaaccg ttgggaccga caatacccgt aacaccgttg
    30481 gagaaatcca gcacgaccct gtccctgaag gacttaaacc ccgatatttc caaatttttc
    30541 agattcataa acaagcagct ttatttaatt gttcaaaatt tgcaaaattt tagcaaaaga
    30601 atataccttt gtaaagtgaa aatacaataa aatgtggttt taatatattc ataccacaat
    30661 atgtagtgct ttgatttttt tagcttttta atgaaattcc gtacttcatc gaaattaaag
    30721 agaaaaacca tgaaaaagca agaaaaggca ggggatgttc ccctgccctg aaagcaacac
    30781 ataaacttaa attattgaaa tatttagcaa ttaatacttc cccgcaatgt agttactcaa
    30841 ctggttaatc agtacatctt tttcagaaat cgtcgatttt acaacatcac caatagaaac
    30901 tatccccaga aatttgcttc cttcaaaaac cggaacatgc cgaatatgct tgcccgtcat
    30961 taataccatg caaccctcca tcgaggtgtc cggcttcacg gtatacatct tattaaaagg
    31021 cgtcatgatt tctttgacca gtgttttggg agatgttttt cctttcagga cgatttttct
    31081 cgcgtaatcg cgttctgtga ttatgccggc aactttacct ttcttatcca cgaccaccaa
    31141 tgccccgatt tctttttctg ccatgagttt aagggcttca aatactgtat cgttcggatt
    31201 aattgtccac atctgcttcg acttgctctt taaaatgtcg ctcactctct gcataatcca
    31261 cacctcctga taaagtctgt ttatttttct cggctgtgaa cttattttaa aagaaaaatg
    31321 ggaatatggc aagcacaatg taaatcctcc cgggatccgc ttgtgctttt tgatgaaagg
    31381 cttgccgatt ccgaaggacg atcactgtca gatttcttcc ccggccttgt agccttcatg
    31441 aatggcttcc aaaatccggc gcggactgcg ggcatcgcct acaacccggt aaggaacggc
    31501 caacgcgtta agcacatcct ccagagtatt ttccgatttc gcgcccatgg cggtcacgat
    31561 cagggatgcc ttttcctcac ttggtttgtc cgcctgaaca tatcgcacga tgttgttttc
    31621 aatcccgagg actctggcgc cgaatattat ttttcccccg ctgtctttga tgcgtttgtt
    31681 cagccagtat ccgtgcgcgg aaaacctgtt caccggcgga agggtctgca tttcaagcac
    31741 cgttacggca atgccgttgg ccagcaggta atcagcggtt tccatgccga catatccggc
    31801 tcccagaaca acagccggac cggcaagttt gatatttccg gtaagcacat cccgcgcatc
    31861 agccacgagt tcggatttca gaccgggaat ggaagcggta accgggtaag cgcccgacgc
    31921 tacgatcacg gcatcgggcg ctgccttttt gatgtcatcg gcgctgaccg gatgagagca
    31981 gtgcaacggc acggaccatt gcttcagacg gcgcagggcc cagtttaccc aatccataaa
    32041 aacggctttg tgcggcggac ggctggccga cagcagttgc ccgcccggct gggattcttt
    32101 ctcaaaaacc tccacctgat gtccccgtgc agccagcacc agggcggcgg aaagtcccgc
    32161 cggtccagcg ccgatcaccc atactttttt cggcgtcccg gccggcaaaa caggtccttt
    32221 gtattcgcgt ccgcattcag gattaaaact gcacgtggtg gatttcatct cgaagctcaa
    32281 acgctcgata cacccctgat tacaggctgt gcagcggcgg atatcctcca gagatcccct
    32341 gcgggctttt tcgataaaat cgggatccgt aagatgctga cgtccgaaac tgatcaggtc
    32401 ggcatcgccc cgggcaatgg cctgatcggc cattgccgga tcattgatcc gacccacgcc
    32461 gatgatcgga acgctcacca cctctttaag cgcacgggcc cggaacagat tgaatccggg
    32521 ttcggtatcc attgaggcga tggacagacc tcccggcgtg gaataaaccc ccacggaaca
    32581 atggatggca tccacacccg cagtaaccat ccggggggct aatctgcgca tcagagaaaa
    32641 atcatatcca ccgcgaacca tttcatccgc cgatatgcga atccagaggg ggaaatcgtc
    32701 tcccaccgct ttgcgcgccg ccgcgatgac ttccagaata aagcgcatgc ggttttcctc
    32761 gcttccgccg tactcatctt cacggttgtt ggagaacggt gaaagaaact gattcagcag
    32821 atagccgtgc gcaccgtgaa gttccaccga atcaaatccg gcctgacggg cgcgtccggc
    32881 cgcctgagca aaggcacgga ctatttcggc aatacgctcc agagacatcg cttcacacgg
    32941 ctgccccaat acggcactgg gtatcgccga aggagcctcg ggttttttgc cgagcaccgc
    33001 ttcaaacgtt tcacggcccg catgatgcaa ttgcagcgcg gccttggcgc cgtgacggtg
    33061 cagcgcggcc ggaatccgtg acagcgcgcc gatgaaatca tcgttccaga caccaatctc
    33121 attgggaaac ccttttccgc gcggatcaac cgcgcagacc tcggttataa tcaaccctgt
    33181 tccgccctgt gccctgcgct ccagataagc cagcaaacgt tcactgaccg tgttgtcggc
    33241 attaccgtaa ccggttgcca tcggcggcat caccgccctg tttttcaaag tcattccgtt
    33301 tatggtaaac ggtgaaaaga gatgctcaaa ccgcattcgt gcctccttgt ttgatatggc
    33361 tgaaagacgt tcgtaaaaaa tgtttaaacg ttgagaatgt ccgtaacgat tttgtcccct
    33421 ttgccggagg aataaaacca aatcggtaaa atatgaagag taaccggtgg aaacagccgg
    33481 ccgtgcgtat cattttgatc attctcagat caccttactg ataagacgat gatccaaaag
    33541 aaaatacgtt ccaggctcaa ctgttttttg cccaaagctt aaattaagat tttttccgcc
    33601 gggaattgca agctttacca gaggccaaac cagtgtttgc tccctgccag ccagatcagg
    33661 gaaatcacat aagcgatcaa aacagccgcc gctatcttct ctttaagggt gatgggctga
    33721 ttctcttgtt tatgaggatc agtggccggc tgctcctgct tgatctcttc ggtcatgggt
    33781 tttcctccaa gcatattttt gatattttca cgtaatatat acggaaatgc ggatcattgt
    33841 caatctttgc ataaacagcc cgattcaaac aacactcctg cgacccttga tttaaatata
    33901 cagtacaata aagaccatct catgaaaaaa gcgcgcaaga accgatcagc ggagccgata
    33961 cgctctcttc atcatccggg ggaaatcggg caccggaggg ctggccatct gttgattgga
    34021 tgagctggtt tcaacacatt aagagctgca aaaaaatcgt tgagggcaaa acatgaaaaa
    34081 aggtgtcgtt taaaatattc cttccagttg tgttccacag tgcgaacatt tgtttttttg
    34141 cagattggtg ccgataattt taaaaccgta gcgttcaatc agcagtttgc cgcagtgaaa
    34201 acagcgcgtg ttttcggcat catcgccggg aacgttgccg ctgtatacat attttaatcc
    34261 ggcctccctg ccgatctgcc ctgcgcgatg aagggattca agaggcgtta ccggcaggtg
    34321 ctgcattttg tactgtggat ggaagcggct gatatgccag ggcgtttccc ttcccagtcc
    34381 ggcaataaat tgtgcgatat ctctcatttc ttcttcgctg tcatttagag acggaataag
    34441 cagcgtggtg atttccaccc aaatccctct ttctttcatt tttttcaaac tgtccagaac
    34501 gggaaccaga cgtgccccgc atcgtttttt ataaaactcg tcgcgaaaag acttcaaatc
    34561 cacattggcc gcggataaat acggcgcgat gatttcgatg gcttcaggtg tcatgaaacc
    34621 gttggtaaca aaaacattct tcaggccgct tgcggctgcg atttttgcgg tatcgtacgc
    34681 cagttcaaaa aacaccgtcg gttcggtgta ggtataagcg atcgttttag cgccgctttc
    34741 tttcgcgcgc tccacgatgt ctgacggcat ggaatcctcg ccgtaaatca tttttgtttt
    34801 tcggggcgct tgtgaaatat cgtgattctg acaaaagtca cagaaaaaat tgcagcccat
    34861 cgtggcaatg gagtaagacc gtgaggcagg gtatacatga aagaaaggtt ttttttcgat
    34921 gggatcgaca ttttgagcaa tcaatgtccc gtaagccatc gaatacagca caccgtcccg
    34981 attgccccgt acaccgcaga caccctgacg ctcgggatgg atcgtgcatc ggtgcgcgca
    35041 aagattgcat cggacgtttc cgtcttgaag tttttcgtag agcatggctt ctttgatcat
    35101 cggtctgcct ctttcaacat gttcctcaca gcttcaggag ccgttttgcg tgtttccgcc
    35161 ttgatattga atgaattcat caccggattc tgtgccgtcc ggtacgtata cgttacggga
    35221 caaaccaagc cgacaaacgc cccgaagggg tttttccgga agggcagcat ctcctcaagt
    35281 tcttcaaaaa aatcgtaaac catggcggga aaataaataa cgcttcccca tttcatggcg
    35341 ttattgccgg ccgagacctc cgccattttt ctgatttcag gtaaactgaa aaaacggatt
    35401 ttttgagaaa aagaaaaacc gaacatttct ttcaggcgct gtctggttcc gacaaaagag
    35461 taattgttca cacaaccgat aaaaacacgg ccacggcaga cccggacggc ctcgtcaatg
    35521 attttttgcg gatgcacggc ggtttccaga caatgaatca tcgtgacaat gtcaaactca
    35581 ttgtcggaga agggaatgtc ttcaggatcg gccagaatca gttcggcacg ttcactgtgt
    35641 ttttgacggg cgatttccag atttgcgccg gaagaatcaa ggcccgtcag agaacaccat
    35701 tttccgcgga acatatccag atggttgccg gtgccgcaac cgatatccag aagacgttca
    35761 ccggccaggg gagtaatcag atccagaacg agctctttct gcctcgaaaa gatgtaatcg
    35821 tccctcggtg tttttaatct ttcagccgga aatccggttt tcttttgatc aaaaaacatg
    35881 gtcttgatcc tacccagatt ttcacaacga tgtaacgatg tattgccgtc ttttccagat
    35941 gcggtccgac agccccctgc cgccggaaat aaagccatta ttttttaaat atatattttt
    36001 ggaaaatatt tttcaaccgc cgatttccga catattccgg gaacattttc gcagactgtt
    36061 ttccgtgcag acaatgtcgt gctgccgggg tttcaacaga acggcatccc agatcagctt
    36121 ttccagttcg gcatcactgc agttttcccg caaggctttt ttcaaatcca cttccgcatc
    36181 attgagcagg caggctctga gtttcccgtc cgacgtcaga cgcatgcgat tacaggttga
    36241 acagaaatgg tcgctcaccg gattgataaa accgatttct ccccgcccgc ctttaatctt
    36301 aaatattttt gccggaccgt ctgatctgtt atgtttgttt ttgaccggtt ccaacacata
    36361 gcgttgccga atgattttaa ttaactccga tccggacaaa taatctttgt cgtaatcaag
    36421 atccgttttg ccaaccggca tcagttcaat aaatcgaatc tgaaacggac gttcctcagc
    36481 caaccgggca aaatccagcg cttcatcatc gttgaaccct ctgatggcca ccgtgttgat
    36541 tttaatcggg aaaaaaccga tttcttccgc ccgcgcaatt cctctcaaaa catcttccag
    36601 acgtccgccc ctggtaatct cataatattt atccctgttg agcgaatcaa ggctgacatt
    36661 aatccggcca atgccggcat caaaaatacc ctgtgcaaac tgttcgagta atatcccgtt
    36721 ggtcgtaaga ctgatatctt tcaatccttc aattttattc aggtccgcaa cgaagtcgac
    36781 aaagccgcgc cggacaagcg gttcgccgcc ggttaaccgc accttgatca atcccatttt
    36841 caccgccagg gaaataacgc ggatcatttc ctcataacgt aaaatatcct catgaccgtg
    36901 cagagcaacg ccttccttgg gacggcagta aatgcaccgc aaattgcagc ggtcggtaat
    36961 ggaaacgcgc aaataattta tctctctact gtatttatcg agcatcattt ttaatacaat
    37021 ccttttttca ttgtgtgata aatagcataa ttatttacac agggccatta aatgtttctt
    37081 gacagcgcca ctattctcca ttaaacaaaa acgacgtatc atcctgcctg ttgttgaaaa
    37141 ataatcacgt caggaagagc aaaacaatcc ggagagatca tggaaaaagt gccgattacc
    37201 aaagcgggtt ttgaaaagct gaaaaaagaa cttgaaacac tgaagaacgt gtccattcct
    37261 gaaaatgtcc gtgacatcga agtggcgcgc gcgcacggcg acatttcaga gaatgcggaa
    37321 tattctgccg ccaaagaaag acaatcgttt ctctacggca ggatgcagga gttggaaaac
    37381 cagctggcca tgtccaatgt catcagtctc aaaggattga acaccgccaa ggttgttttc
    37441 ggctgttatg tcagcctgca ggatattgat agcggcgaaa aaattaaata tcaactgctt
    37501 ggcccgtatg aatccgatat cagccggaat aagatttcgg tgacgtcgcc aatcggcaaa
    37561 gccctcatcg gtaaaaatgt cggcagcgaa atcaacgtca agacacccgg cggcacacgg
    37621 aatctcgaaa tcaccgatat ttccgttgaa gaagaataac gttcctcatt tatacgcctg
    37681 cggaagccga taccatagct tgctcaattt cttcccggac aacgccatcg ctgtttttca
    37741 aaaacctccc aagcgcctcc acagcctcat taccgccgat acgccccagc gcccaggccg
    37801 ccatgcatcg cacgcgttca tcggaatttt catgaaacgc ccggatcaga tcggcgacat
    37861 aaacgggatt tagagaattg cccatggcgc gagcaacatt catcttccag cgccagagat
    37921 catccggaga catataaaac atatgcggcc atattttctt ttcaaaatat acggcatcca
    37981 tatgcagaag acgggataat ccgaaatccg acgcctttgc tgaaacccgt tgattgaccg
    38041 gcttttcctc agccatccac ggaatgtttc gcgggcaaac gttttgacaa cggtcacaac
    38101 cgtaaatgta catccccatg ggctcacgaa ggtcctgcgg cgtcaatccg gagccgtaat
    38161 aggtcaggaa agaaatgcag cggcgcggat caatttttcc tccgcccttg agcgcgcggg
    38221 tggggcaggc cgccacgcat gcattgcggc accagtcggg acagcccatt gcgacggatg
    38281 gttcatccgg tgtaaattca caatcaacaa ccaaggcaat tggaaaaacg aaagaactgc
    38341 cgcgggcgac gcgccgtgca taaaaaatgc agttgttgcc aaaggtcccc aaaccggcac
    38401 gggcggctga taaacggtgc ggcagatgaa acggcacttt ggaattaaca ccgtttgcct
    38461 tcaaatattt tctaaaggct ttaattttca gcgccaaacc gtcttgggta atccggtcgt
    38521 catcgagata gcaccggccg aaatgacgct ccatcggctg cggaaaggat tcttcaaaat
    38581 atgattccag aacaacgata atcgattttg cgccggcaag aatatttcgg gggtcggttc
    38641 cggccagcag ccccaggcca atcgcttcag cccagccgta ttcctcctgg tgcgtcagca
    38701 gatagtcttt ctgcgaacca aacggctccg cgcccgtgaa accgatatcg gcaaatttca
    38761 ggcgctttgc ttcgtcaatg atttcttctt tggtaagcat ttggtgtcct ttcgttgatg
    38821 ttttgctttg ggctttagat agttgcttct gttcagcaga aaatctttgt aaatccggta
    38881 gtgctttgtt ttctcgtaca caattttcgt caacggcgat ctgtcgaaat tataaattgt
    38941 tgcctgtggg caggccagta aaagcggcga atgcgtggcg atgatgaatt gggcatggcc
    39001 ggcggcgctc atgcgctgaa gaatgtccag caggagaatc tgtgactgcg gcgacagagc
    39061 ggtttccggt tcatccagca gatacaggcc cggaatccgg taacggctgt aaaaaaaaga
    39121 catcagggac tgaccgtgcg actgagtggt cagcgatcgc cctccgaaat aattcaactg
    39181 gtcgcgatcc gctgacgccc attcgtcgag tttttcagcg aagtactgga aattcttgga
    39241 tcccaaaaaa gacccgggca ccggctcgtt tgcccagatg accgacatat accgatgaag
    39301 ctgatcttca taaggattgt aatccggtct ggttctctgc gtgtcacgcc agatatggat
    39361 ttgacaggct tgagccagcg cctctatcag cgtggatttt cccgttccgt tttcgcccac
    39421 aaacagcgtc accggcgtat cgaacaccag ccgttttgtt gccctgaaca gcggcaaatg
    39481 aaaaggatag tggtcttttg tcggatattt acgagaatga aacatcgccc cggtcagatg
    39541 catgtcttga gtccgataca aactattatt tggaaacttt atacaccaac cttcacatga
    39601 gcacaagggc agacatcgcc cggttaaatt taacaatgca ccgcgaaata aattgacaat
    39661 accgtttatt gtttagtaga aatcaaaccg tgaaacaaaa acaaaatttg aaggaacagg
    39721 ttgtgtctca tgaatgctaa gtccgcactg attatcgccg ccatcgtttt tatttttacc
    39781 ggttgtgcct cggaaaatat acaggtccgg aagctcccgg actcggccat caacgcgggc
    39841 cttgaagacc tgaggcagga ggtggcggac aacggtgtac cgggcgaatg gtttgacgat
    39901 cagatggacc attatgcgtt tcggcttcac cacaacatcg acagattttt cagaaaatcg
    39961 gcggaaaaga aaacagacag tgaaaagaaa cgcagcctcc tgtggtactt ctccatcttc
    40021 ggtgtggaaa caaaaatcga aaagggcaaa acattcatcg aggattatac cggcatttta
    40081 gaacgggccg aggcgaagca cgggattcac aaggaattga ttgccgccat catcggcatg
    40141 gaaaccaatt ttgctcaacg ccgttatcgc ggaaaatttt acgcttttaa ttccctggtc
    40201 tcccaataca tcttcaccga ccggaagaaa tttgccgttc gtgaaataac atcgctctat
    40261 aaattttcag aaaagaccga ccggtcaccc tggtatttca aaagttcgta cgccggtgcc
    40321 atcgggtggg ggcagttcat cccgtcgtca ttgctgtctt tctttatcga cgaaaatgga
    40381 gacaacgacg acattgatcc tttttccata gaagatacga ttttcagcgt cgaaaactat
    40441 ctttaccggc acggactctc cggcaagaac gttcataaac gtcgcagccg gtataaagcg
    40501 gtttatgcat acaaccacag cgacgcttac gttcaggcgg ttctccatat ttatgacggg
    40561 ctgaaaaact aacgggtccg ccgccgaccc gactttgact gggatctcag acctctgtca
    40621 ggtcagtttt tcttcttttg gctgtacgac acgaagctgt gcaggaacca tccgatgata
    40681 aaaccggtca gcaggataaa aaacaggaaa atcgcgcgcg acatcgacag ttgccagaac
    40741 agaaaatgta ccgtcatcac ttcgatgttc tgaaccagaa agatcaaagc cagcagggcg
    40801 aagatggcaa ccagtccgat tttaaaattc attctttgcc tccttttctg ttgaggaacg
    40861 atgaacgatc atttattttg attccttccg aagatgatac ttgctcattt tcaactgaag
    40921 ccccttgcgg ctcaaaccca tttttttcgc cgcctgcgtg atattgccgc cggattcatc
    40981 aagcgccctg gcaatcatct gccgctcaac attttccacc tgatcgcgca catattcctt
    41041 aaacggtttt tttataccgg gggataaagc cgtattgaca tgatcaaccg attttttgaa
    41101 gtcatcgggt acgtcgccgg acataatccg gcatcccctg gccagcagca tcatcctctc
    41161 gatgaggttt tccagttcgc gaatgttgcc cggccagtca taacgtaaaa gaatgtcttt
    41221 gacctcattg tccatgccga cagagatccc gagtttttta ttgaatttat cgaggaagta
    41281 atccgcgagt gacagaatgt cgtcttttct ttctctcagc ggcgggatat cgatgggaaa
    41341 aacgttgagc cggtaataca aatcttcgcg gaaatttccc gtctgaacct gacgcagaag
    41401 atgttgattg gttgccgcaa taatgcgcac atcgaccttg atggtgcgca gcccccccac
    41461 cctttcaaat gcctgttcct gaaggacacg cagcagttta acctgcatct cgcgcggaat
    41521 ttccgccact tcatcaagaa aaagcgttcc tttgtgggca agctcgaatt tgcccggctt
    41581 ggtcacggcg gcaccggtat aggctccttt ttcataaccg aacagttcac tctccatcag
    41641 cgtttcggca atggctgcac agttgatctt gatcagggga ttatttttgc gttgcgaatt
    41701 ataatggatg gcgtccgcca ccagttcttt gcccgtcccc gtttcgccgg tgatcatcac
    41761 cgtggtggta gtttgcgcta cccggtgaat ggcctcaaag atttccatga tggcggggct
    41821 ggtcccgatg atgcccaggc gatcgatttc cgaaggcgcc atggaaaatt cactttcttt
    41881 caacgtgctg gttctgatcg ctttagacac aatattcttc aggtcatcaa ggtcgaaagg
    41941 tttggtaata taatcgaatg cccctttttt cagcgcttcc accgcagtgg caatcgtccc
    42001 gtgcgcggta atcatgacaa ccggaatttg cggatggttt gcagaaatct gggtcaatag
    42061 atccatgccg tccattcccg gcattttcaa atcggtaatg accaaggcca tcttgttcga
    42121 ttgaaggatc tgcagggctt cccgcccgtt ggaagcggag gacacttcaa aaccttcttt
    42181 cttcagcatg gccgacaaaa ccagccgcat gttcagttca tcatcaacga ttaaaatctt
    42241 tttcatagtt tgcaaatagt atgctttgaa attttaatca agccggatta tcggatgact
    42301 caaccggatc ttttcagctg aatcacaacg gtggtgccct gagacgcaat cgactgcacg
    42361 ctgatgcgtc cgttgtgttc cctgatgatc ttctgacata tggccagtcc cagaccgaca
    42421 cccctttcct tggttgtgaa aaacggcttg aaaatgtcct tgacgtattt ttgatcgatt
    42481 cccgtacccg tgtcccggat ggtgagcgct aagtcttcgg ccgtttcgga tttctggctg
    42541 ctggtgcaaa acgtcagggt gcctccctgc ggcatggctt cgatcgcgtt taaagcgata
    42601 ttcagaacga cttgcagaca ctgctgttcg tcaacggccg caaacggcag cttatcttcc
    42661 aggtcttcaa cgatggtgat gtgctccaac agtttgtttg cggcaataac ggaaatggcc
    42721 cggcgaatga tggcgttaat atcctgtgtt ttgagctgaa gctggtaagg acgcgcgtaa
    42781 tccagaaatt tgctcaccac ggtatttaat ctgtccgttc cttccacaat gacatccaac
    42841 agttccttgt gttcccccgt cgacgcctcc gttttcaggt actgcgccgc tcctttgatg
    42901 gaccccaaag gattgcgaat ctcatgggcg agaacggggg ccatctcctc cagcatcagg
    42961 gctttttctc tctccagctt ttcgtcaaat tcataaagag acgtaaaaat atctttactg
    43021 tcgggataga aaaccccgaa gatttttctg aaaatcattc gcatcggcga cagggaaatc
    43081 acgacgagaa aagaggccat cagcaatccg gtcagggggg gatcaccgtt gaaaaacgaa
    43141 acagtaaaat aaaaaatggc ggcgccggca agagtgctga gcaggataac cagcgaccgg
    43201 gccaaaaagt catgtaattc caaaagttgc ggataggcaa taatcagcaa aataaaataa
    43261 agcagtgcgg atgataccat gccgttgatt ttcagcaagc tgaatcccca gaaatttaat
    43321 aaatccaaat aactcagaat caaagctacc gggcagaaaa ccagcaggta accgaaacgt
    43381 cttttttccg tacctgacgg caatgttccg acatgcccaa ggagggccaa atagcagatc
    43441 atcaacgaga acagcacata agccgcgatg acattatgaa aacaggtttt ttcggataac
    43501 ggcagaaaaa caaaaacgac gccgcacaga ctgataaccg caaagagcga aaaaaaattc
    43561 cggaggataa tcgattgccc cttggtcaga tgatggaaaa accgcagcgc cgccggggca
    43621 atggccaggc tgcccagata ttttattttc aagagaaatt cataatggaa aatattttcc
    43681 agcgtcacgg cggcctgtga aacaaaaacc gccgcacata aagcggcaaa ggcggactga
    43741 agcctgttcg tcttcccggt aatcatcaga gaaacggcaa tggtgaaact ggtcgccacg
    43801 agaataaatg tatccatagt tacctgacaa agttttttta aagattgttt tgcttgattg
    43861 aatacattgt ccggccggct ccattctgaa catcggcccg gatccggatc gttttatttg
    43921 tccgcggttt tgccgaaacc ggccagggat attctgaaca ggaatctgac atcgtcatcc
    43981 gtatcgctga gatcaaatcc cagactccag cattgcgtgt gataaacaaa tcccagagaa
    44041 ttttctattt tccgggcgtc ctgcaaatcg cgtttcagaa caaaggtgct gtagaaatga
    44101 tcggtaatga cggccttcac acccaggttg atttcctcca ccagaccttc cgtattccgg
    44161 taggctacaa gcagagcgtc gccgcgccag tctctgaggc tcagatcata actgttttgc
    44221 ttccatccgc tgtaaacatt atatttatcc ctcatccgga aagaaaaata tttatgaggg
    44281 ctgaaatcga attcaatgcc catgtcggag aaaggccgcc tctcgggtgc attccgcgcc
    44341 atatctctcc gggcctcatc gatatcgtac gcctgaaaca ctttcagccg gaaaaactcc
    44401 agatagctta aggacccgcc ctgatctttg accctcgccg tcagtgtgtt ggtcagcgac
    44461 caggcaacgg cattctgtcc gttcagcgga tcgctggaca acgatgcaag gggcattccg
    44521 aatggcgata acgccggcag ataataatcg ggaacatcct ctgcggaaat agaaggcata
    44581 taggagtaga tgatttccgg tttgatctcg tgtctgattt tttcccagtg tttcccgttg
    44641 agatggaata cccggaaaag ctggctgcca aaagacaccg aggcattata aaccgagcgg
    44701 tcagccgttt tctcttttaa gccgttttca tcatcatccc ggctccagaa cgtttctttg
    44761 aacgtaaact gggggataat tctgacgtag tcggaaagat tgaccggcat ggagagagat
    44821 ggcgacaaat cggcaaaatg tcccttatga ccctccttct tgtataaata atcgtaacgg
    44881 ccggccagct cataataaaa aggcgtatgc agcaagggct gcttgacgcc ggcaaaaatg
    44941 atttccggat atctttgcag cgtctgatca ttattgacag cggcaaaatc gtcagccgca
    45001 tgcattaatc ccgtcacatt atacttggac catcctttat acacgcggat ggttgaatcc
    45061 aggtaacgca gcgatttgtc gccttcgaaa gacacattcc gaaaacggtc ttcggacgtg
    45121 ctgttaaaat gctcccgata ataattataa gacgaaaaat ccctgaaata ccacttgtcg
    45181 gacacctttt tcaggtccgt tcggagataa aactgcgaat cgagcctcgt ctgatggtgc
    45241 aggaaataag accagcgttt gtgcattccc tgccagtccc gtcggagagc gtcatcctcc
    45301 gtttccgtca cctgcttggt atcctccaga taatcgccgt aaaacgtgcc gaaggattta
    45361 ttgccgaagt catatctgaa ttccgcccct tctttaaatc ctcttttttc aatgaaacgt
    45421 tgataaaacg tcgcatccat ccaaggcgaa acggcccaga aaaaaggaag ctccacgtcg
    45481 attccgtctt tatcgcggga atacgccaga tagggaagca gaaaaccggt ttgccgcttg
    45541 gttttagccg gaaaaagaat atacggggaa taaagcaggg ggaggctccc tactttaaaa
    45601 caggcgtttt tcatcaaacc gtagccttca atggtcacct tcatttcact gccggcgatt
    45661 tcccaatccg ggttactgcc gtcacaggtt gtggcagagg gattgtcgat aaaataggtg
    45721 ttttctcccg ttttctctat gatattgcca cggacacgaa aatggtttct ggcatagaaa
    45781 gcgtcagcat tgaacgcggc gccggtttta tcttcaatat tgaaccatag gcgttcaccc
    45841 ttcagcgtat cctctccgat tttcaaaagc gtgttgccct gcgcccgggc aacatgattc
    45901 tgattatcca gttccacttc atcggcgtat aaggaagcgc cgctgtaaaa gacggacacc
    45961 tggcccctgg cacggtataa atcgctgaca tggtcgtaat ccatggcgtc ggcttcaatg
    46021 ctgacagggg acttttcgtt cgtgtgaacg gatgccggga aatttttttt ctgaatggaa
    46081 ggtgatgctt ttcccgccga ggatttttgc ttgcctgcac aatccgcggg aaatgaccac
    46141 aggatgatga tcaccgcaac cagacaaaac gtaaagacag gctcaaggca gccggagaaa
    46201 gaagccagtc cgttgtgttt tatcggcttg acgttaatca gcgtgttgat gttcatgaaa
    46261 ctttcttgcc ctgtactttt ttgctttttt cattatcaca gaagactaat ttagaaaacg
    46321 tctgttttac ttcgccggca agataaaaag atccggtggc gcagattagg tcgcgactcc
    46381 cggccagaga cagacccagt tccagagcct gatgaacact ttgagagaca acgggcatgc
    46441 ggcccaatga gcgtatcgtt tgactgaccg cggcaacgga agcggctctt tgtgccttga
    46501 gcggcgccag aatgatcacc cgagccagcg gaactatttt ctgaagcatt ttacggtaat
    46561 ccttgtccgc cagaacgccg aatataagaa tcagatgacg aaaggaaaaa tcttttttca
    46621 gtgaccggca caacgcaccg atgcccgcgg gattatgcgc tccgtccagc agaacccagg
    46681 ggcttatgtg taaaacctcc agccggccct cccagcggac attttgaagc ccggcgcgaa
    46741 cggccgtttc tgagacggga aatccttttc gagcgcacat ttcaacagcg gccagagcca
    46801 gggcggcatt ggccaactga tgttcgcccc gcagcggcac ggtgagattt tttacatcgc
    46861 tgtacactcc ttcatacagc aacgatccgt ttttttgtct ggcgagtctg aaatcgtgat
    46921 tcaaacggta caaggaagca ccgcgctgca gacagatatc ctgcaacaat cctaagattt
    46981 tcttttgggc gactccggtg acgcacagac catgaggctt gatgattccc gctttttcca
    47041 cggctatcga cgtcagggta cgacccagat aggccgtgtg atcaaaagca atgtttgtga
    47101 tgaccgaaac gagcggtcgg caaacattgg tcgcatccag tctgccgccc aggcccactt
    47161 ctaaaacggc aatatcggct tttcgcgtct tgaaatattc aaaagcaacc gccgttataa
    47221 actcaaagta cgtcaacggc tgaacgactt tctttctgac gcagacaatg attctgtcga
    47281 aatctttccg ggatattttc cttccgttaa cggtaatacg ttcccggacg tcaatcagat
    47341 gcggcgatgt gtacagtccg acgcaataac cggctttgtg cagcatggcc gcgatcatcg
    47401 cagcggttga tcctttgccg ttggttccgg taatggtcac cgccttaaat gatttttgtg
    47461 gatgtcccaa acgcgacagc acgctggtga tcgccccgag accgaaccgc attttattga
    47521 tgttcaaact gtccaggtat tgagatgttc ttgaattttt catggaatgg tcgacggccc
    47581 ggctaatatc tgtccttcct gaatctccgc aaagctagca taccctctcc ggacagggaa
    47641 tgaaataaaa ttaccggttc tcagacaaca gactctgcca ggtgtgacgc tccagaaaag
    47701 aaccggggac attctgctcc atttcgtcat aaagattgaa ggggacggaa aaagtcatca
    47761 gatgagactc gcccagttgt ttgcgaacat aaacccgtgc agaaaggtcg gtaagaccga
    47821 caacggcacg ggggtttttc aacccagcct cgcggtaagg ataaataccg atggcctggc
    47881 aaccggcggc gtaagggatg atgacatttt cattgccttc acgcccgtag ttcgccagaa
    47941 cggttaaagc ggacaactga tccggattca cgaaaaaaat gacggtctgc ggcttttcgc
    48001 cgtcctgatc cacgtcagac agcggttgaa aaacaacaaa cggtttgggg atatctgtca
    48061 gaggaagatc cctgataaat ttttcaacaa gctgaggatt ttgaacgtag cgttcgccgt
    48121 tcagaaaatt atcatggctc tccctggtca tgaacggttt gacttttgcg gccatttccg
    48181 gtccgcccgg acgtcgggca tttccgctgg aaagaaaata acagaagccc tcctcaccgc
    48241 cggggaaatt tttgtactga ttaccgaacc ccagaccgac gccgccgccg aaacaaccaa
    48301 atgttttcct gtcggcaaca gccgcacgac ctttggcggc gtgaaccgcc agccacatga
    48361 cgcagcccca tttcccttcg gcaaactgca tggcatcagc aggtttttca acggaccaca
    48421 acagcgctac cggctggtat ttaagtttta tcgcttcggc aattctgctt ttcatgacct
    48481 gctagcctct aagaaaacca tgcatcgacg atgaaatcct gatcgggcaa aagaccactg
    48541 tgtcttttca cctgaatcgg gattttcata acaaaccgct gatctccggg caacattaat
    48601 ccgggatgac tttgaaaacc tcatcgccgg gccggacacg atcggaaact ttgatgccga
    48661 taaaatcacc ggctaccgct tttgggaccg attgattgtc cacctccatc gactcaatca
    48721 catcggtaaa atcggtggtg tgtccggaaa atttcaggct gtcgccgacg gcaatctcgc
    48781 catcggttat ctttaccgcg gccactgaag gttttgagaa aaacttcatt acttcgccga
    48841 tcttcttttc tgccataacg aaacctccat tgttagttta tctgaaagca tatacccgta
    48901 ccggacaatc gatgatatcg agtactgctt cgtttcctgt tttgagcgcc tgcgtctgct
    48961 ggatcatact caacgatccc ggtctgccta atgcaagcgg cagtgtcatc acggtaagct
    49021 caccgccgtt aaatcccatt acccgcctca tctgctttgc agcggttatc gtttcaattc
    49081 ggcgttcaca atactgcttt tattatcaaa aaacaaccac aaaaaaaggc gggggacaac
    49141 ccgccgcctt tttgatggtt tattcatgga aaatcttact ctgctttttc cttcgctttc
    49201 gctctggatc tggctttggg ttttgctttc tcttttttct tctcgtcatt aataaattct
    49261 agaatggaaa ccggagcatt gtcgccatag cggtagccgg ctttaactat tcgtgtatat
    49321 ccgccggcgc gattgcgata gcgttccgtc atttcaccga aaagcttacc gacggtttcc
    49381 ttgtcccgga tcaccgccag tgcctgacgg cgtgcatgca gactgccttt tttacccaag
    49441 gtgatcattt tctccgccag ctttctcaat tcttttgctt tggcatctgt cgtgcggatg
    49501 cgttcatgtt taatgacaga agtcaccata ttgcgaagca tcgcttcctt atggctggac
    49561 gtcctgccca gttttctgcc cgctttaccg tgatacattg ctgatttacc tctttccttt
    49621 tataataaat acaacgtgat ttttgatatt gaccgccaat gctaattttt cgaaccgata
    49681 tccattccga acgtaagatc atattctgcg agaatatctt tgatctcgct caatgatttt
    49741 ctgccgaaat tcttggtttt aagcatttcc gcttctgttt tcttcaccag ttctccgatc
    49801 gtgttgattc cggcattttt cagacaattg gccgatcgga cggacagttc cagatcttcc
    49861 acggaccgtg atagaatttt gttgatgttg tccggttcat cctgtttttc cgggagaatt
    49921 tcctcttcga cttcttcaaa attaataaaa acatccagtt gctgtttgag tatttttgcc
    49981 gaataagcga tggcgtcttc cggtttaacg ctgccgtccg tccaaacttc caaggtcagt
    50041 ttgtcgtaat cagcaatctg accgacacgg gcgtaggaaa cgacgtaatt cactttttta
    50101 atgggtgaga aaacagcgtc tatattgatg gttcccttgg gctgatccgg atccagttcc
    50161 tttttcgccg ggacgtatcc cttgcccatt tttaccatca ttttcgcttt gaaagaaccg
    50221 ctggaaagcg tggcgatgtg atgctcagga ttcaggattt ccactgtgcc gtccgtcata
    50281 atgtccgcag cagtcacttc gcctgcgcgg ctgacatcga tgctcacttc cttgatattg
    50341 gcctgcccca gtttaaagcg cacacctttc aggtttaaaa taatatccgt gacatcttct
    50401 ttaacaccgg agattgtcga aaattcgtgc aaaacccctt caattttcgc tgatacaatg
    50461 gcagagccct gtatggaaga aagcagtacc cttctcaggg cattgcccaa cgtgatacca
    50521 aatcctcttt ccagaggctg aatggtaaac tctccataga aattcgtatg tgtcgcttca
    50581 tcaacatcaa tacgcttggg acgaatcaaa cttgaccagt tcctttgcat aattaaaccc
    50641 atccttatag tagctggttt atgaaaatag tttttttact tcgaataaag ctcgacaata
    50701 agctgttcct gtatcggcat ggttaaatct tcccgcaccg gcagtgtttt aatcttggca
    50761 ttgaaattgt ctttatccaa ttccagccat tgcggaacac ctcgcctgac aaccgtttca
    50821 agggctccca tgatgcgggt caccttgcga ctgttttcct ttacagaaac ttcatccccg
    50881 gccttgagca gataagacgg gatgttaacc ggtttgccgt tgaccagaaa atggccgtgg
    50941 ctgatcaact gccgggattc aattctggaa ttggcaaatc ccatgcggaa aaccatgttg
    51001 tccattcttc tttccagcag tatcagcaaa ttggtgcctg taataccctt ttgcttatcg
    51061 gccttttcga aataacttct gaattgtttt tccaaaagcc cgtacattct cttgagtttt
    51121 tgtttttccc gcaactggac cccataatca gaacgctgtt ttttatgcat ctgaccatga
    51181 tctcccggaa cataatctct tcgttcaaag gaacattttt ccgagtaaca ccggtcaccc
    51241 tttgaaaaaa gttttaatcc ttcgcggcga cacaacctgc atgaggaatc cgtatatctt
    51301 gccaaaaatt gcctccctat atttccttga aattaatatt gttcgttaat cgttacaccc
    51361 ggcgacgttt cggcggccgg catccgttat gcggcacggg cgtcacatcg cgaatcaacg
    51421 ttatcgtcag accggcgatc tgcagcgagc gcagtgccga ttctctgccg gcgccgggac
    51481 ctttgatata aacctgaact tttcgcatgc cctgttcttt ggctttgcgc accacatctt
    51541 ccgcagccat ctgagcggca aaaggcgtgc tcttgcgcga tcctttgaat ccctgcaatc
    51601 cggatgacga ccatgcgatc acgttaccac tcatatcggt tatggtgaca atggtattgt
    51661 taaacgtcga ctgaatatgg gcaatcccgt cggtgatgtt ctttttttct tttttcttgc
    51721 cggtttttct gactgcctta gccattctac ctccacttgg aaatcataaa tttacttttt
    51781 cttgcccgca atggcgcgcc gcggaccttt tcgggtacgt ccgttggtat gcgttctctg
    51841 gccccggacg ggaagcccct tgcggtgtct caacccacga taagttccga catccatcag
    51901 acgtttgatt gacatggata tatcccggcg cagatcgcct tccactttgc cttctttatc
    51961 gatgatgcct ctgatcgccg atatttcgga atcggcaaga tcatctgtcc gtgtgtccgg
    52021 gctcaccccg gcatctttca ggatttgctt tgctctggtc ttgccaatgc cataaatata
    52081 cgttaaagca atttccattc ttttattttt cggtaaatca acacctgaaa ttcgtgccac
    52141 ctaaaacctc ctgaatataa aaaaactaac cctgacgttg tttatgcttg ggattttcgc
    52201 aaatcacccg caaaacacct ttgcgtttga caattttgca tttttcgcat atttttttaa
    52261 ccgatgatct aaccttcacg atttaaactc ctcattttta cttcgtcctg taagtaatcc
    52321 ggccccgggt caaatcatac ggtgaaagtt ccaccgtcac cttgtcgccc ggcaggatct
    52381 tgataaaatg catgcgcatc tttccggaaa tgtgcgcaag aaccttgtgt ccgttgtcca
    52441 gttccacccg gaacatggca ttgggcaggg gttcaatcac tcttccttcg acttcaattg
    52501 cttcctcttt ggccataatt ccttttgtat tccaaagcta ataattcagc cggctcaata
    52561 tgtcgggacc atgctctgtg atcgcaatag agtgctcaaa gtgagccgac aaacttccgt
    52621 cttttgtgac caccgtccag ccgtccggca acacctgaac cttgtatttt ccctcattca
    52681 ccatcggctc aatggccagg atcattccct tcttcagttc gatcccgcgc cccttctggc
    52741 caaaattggg aatctgcggc tcctcatgaa gatccttgcc gatgccgtga ccgacaaagt
    52801 cgcggaccac cgaatatccg gcttcctcca ccacagattg cacagcagcg gatatatctc
    52861 ccagcctgtt gccttctctg gcctgtgcga tgccggcgta taagctctgc tcggtaacct
    52921 gcatgagcct taaggctttt gccgtaacct ttcccaccgg cagggttacg gttgcgtcac
    52981 cgaaaaatcc ctgataaaaa acgccaaaat caagcccgac aatatctcct tcctccaaaa
    53041 cccgctcgga aggcattccg tgaacgacca cttcattgat cgatacgcat aaagagtgag
    53101 gataaccatg atagccttta aatgccggcc gggctcctct tttttccgcg atctcctcgg
    53161 caaatttatc caggtctttg gtcttaacac ccggcttcac cttttctctc agtctgctta
    53221 aaacctcggc aacaattcgg ttactggctc ttgccctttc aatttcttcc ggaagcttca
    53281 gaatgaccat tatattcttc cgttatctcc ttcgggcaat atggcccttc ttcatgaatc
    53341 cctcgtattg ccgtgtcaga agatgcgact cgatctggct ggccgtatcc atcgccaccc
    53401 cgacaacaat caacaaggcc gttcccccaa aataaaaagg aacgtttaac tgggcaatca
    53461 gaacgctggg cagcacgcag atgatggaaa cgtaaatcgc cccgctgaaa gtcagttttt
    53521 ccagtacgtt ttcaatatat tccgcggttt tctttcccgg tctgatgccc ggtacgtaac
    53581 cgccgtattt tttcatattc tccgcaacgt cgtccggttt gaacgtaacc gccgtataaa
    53641 aaaagcagaa aaagaagata aacgccacat aaagcacttc gtacgccagt cttcccggca
    53701 ccaaataggc ggcgatatcc ttcatccagg tctgcggagc aaaattggct atcgttgccg
    53761 gaaacatgat caccgacgaa gcaaaaatcg gcgggattac tccggacgta ttcactttca
    53821 gaggcagatg ggttgtctgc ccgccataca ttttccggcc aacgactctt tttgcatatt
    53881 gaaccggaat tcggcgctga ccgccttcca caaaaacaat cgcccctacc accgcgatca
    53941 tcaaaacggt gattaagaga ataaccagta cgcccagttc accggcagtg gccagcctga
    54001 aggtattgcc gattgccgcc ggaatccggc aaacgatacc ggcaaaaatg atcagcgata
    54061 taccgttgcc aatgcccttt tcagtgatga cttcacccag ccacataata aacgccgtac
    54121 cggaggtcag cgtaatcatt gtcatcagca caaaaccgat acccggattc aaaacaatcg
    54181 gagctcccga tggcccggcc atctgctgca gaccaaaagc aatcccgaac ccctgaatca
    54241 tacttaaaag aaccgtcccg taacgggtat attgcgttat cttgcggcgg cccgattcac
    54301 cttctttgga cagtcgctcc aaatgcggaa ccgcaatcgt cagcaactgc agaataatcg
    54361 aagaactgat gtagggcata atgcccagag caaaaataga aaaattgctg aacgctccgc
    54421 ctgcaaacat atccatcaga ccgaacaacg atcctttggc atgttcaaaa taagccagta
    54481 acgctacatt atcaattccc ggagtgggaa tataaacgcc gattcgataa acacccaaaa
    54541 gaagaatcgt gaataaaatc cttctttgca actccggaat cttggataca ctgcccagcc
    54601 cttctaacaa atcaaattac ctcttccacc gaaccgcccg cagcgatgat tttatctttt
    54661 gcggccgggc tgattcgggc aatttttaat gtcacaggaa aatcaatgtc tcccttggcc
    54721 agaattttga tatgggcgcc ttctgtttta atcaggccgc tttgcagaag agccgcctcg
    54781 tcaatcaccg cacctttgga gaattttctg cacaaatcca aaatattgac ggcaatatat
    54841 tcttttctga aaggattgcg gaaccctcgt ttgggaagac gacgcgccat cggcatctgg
    54901 ccgccttcaa aaccaaccct tacctttccg ccggaacgag ccttttgtcc cttatggcct
    54961 ttgccggatg ttccaccgtg accggatgca tttccccgcc ccacgcgttt gcgttttttt
    55021 gtcgcgccgg caggagcctt caacgaaccc aaatccatat tctactcctt tgactcttcc
    55081 acggaaacta aatgcgtaac cttattgatc atcccgcgaa tctgcggtgt atcctccaag
    55141 gttacggttt tatttaattt ggtcagcccc atgcccttta aaatttttct ctgcttttcg
    55201 ggtcggccga ttacactttt cacttttgtt atttttaaca ttttacccac gatacgctac
    55261 ctcaccgtat aattgacaga gctatttgcc tctctgtgcg gcaatttctt cagggttttt
    55321 caactgacaa aggccttcga aagtggcttt taccaagtta tgcggattat gagatcccag
    55381 gcatttggtc aaaacattgt gcacaccggc aacttccagc acggcccgca cagcgccgcc
    55441 ggcaatcaat ccggttcctt cagatgccgg cttcagcaaa accttccccg caccatagcg
    55501 gccgatgact tcaaaaggga tggttctgtc cacaacggcc accctgacca tatttttctt
    55561 ggccatatcc atgcccttgc gaatcgcttc aggcacttca tgagcttttc ccaatccggt
    55621 cccaatcatt cctttgccgt ccccgacaac aacaatggcg ctgaaacgga aacgccttcc
    55681 gcccttaacc acttttgccg ttctgttaat ggcgacaacc cggtcaagga gctccatgtc
    55741 tttcatttct ttttctttct tgtccaaaga ttctttcctt tattcgcttg attgattaat
    55801 aaattaaaat ttcagaccgc tttccctggc ggcttcggcc aatgccttga cgcgcccatg
    55861 atagagaaat cttccccgat caaaaaccac cttctcaatg cctttagctt ttaaacgttc
    55921 ggcaatgagt ttgcccactt ctttggccac atccaccttc tttttgcctt tcagcctgct
    55981 ttccagttcc ttgcagagcg tcgaggccgt tgccaacgtg tctgccgtta catcattaac
    56041 ggcctgcaca taaatatgcc tgtcactccg gaatacggaa agacggggtc tctcctgagt
    56101 tcctttaatc tttgatcttg ttcttttttc tcttttttct ctgatcttta atgtcttttt
    56161 cgaagccaat ttcctacctc tttacggcaa tataattttt ttaccaatta agcaccggca
    56221 gacttaccgg ctttgcgttt gatatactca ttcgcatatt taatgccctt gcctttgtac
    56281 ggttccggtt ttctcaaagc cctgatttcc gccgcaaccc tgccgaccag ctctttatca
    56341 ataccggaaa cgacgatatt gacctgttta tcaattttga tatctattcc cttgggaata
    56401 tcgtactgaa ccggagacga gaaacctaaa ataaatttca aacttttatc gtttgcttca
    56461 gcgcgataac cgactccaga tatttccagc cgcttttcaa acccggtggt aacccccttg
    56521 atcatgttat caatcaacgt tctggtcaaa ccgtggtagg aacgggtcgt cagagcttcc
    56581 gattttcttt caaccgacag tgtgccgtcg ctgattttga tggcaattcc ttccggcatt
    56641 ctggaagaaa gcgccccttt gggcccctcg acctttaccg tatcagcact ctggctgatt
    56701 ttaacattct tgggaatact tactggtttt ttacctattc tcgacatgaa ccacagctcc
    56761 tgctaatatc ttgatgttca aattaccaaa cgttgcagag gatttccccg ccgacattca
    56821 attctctggc ttccgcgtcg gtgataaccc cccgggacgt cgaaagaatg gaaattccca
    56881 acccgttgag aaccttggga atattgtccg caggcacata aattctgcgt cccggtttgc
    56941 tgactctttt aatattcgta atcactgtac gcttggcatc gggatattta agaattattt
    57001 ccagcatctg ccggcctttt tcatctttga ccttttcgta attcgtgatg taacctgttt
    57061 ttttcaatac accggccaca ttcacattca cctgagacat gctgacacgc acggtcttga
    57121 accgcgcctt attggcgttt cttattctgg tcagcatatc ggcaatagga tccgtcatcc
    57181 ccatgctttt tttctcctta agctaaagtt accaactgga tttaataacc ccggggagtt
    57241 ctccctgcag ggaaagttta cgcaaacaaa ttctgcacat atcaaatttt ctgtaatacg
    57301 cccgcggcct tccgcaaaga gggcatcgat tgtattctct gactgaaaat ttcttctctc
    57361 gcttcgcttt cgcgatcaat gactttttag ccacgcaaaa ttcctccgga gcttcacaat
    57421 ttactgttta aaaggaacgc ccatcatttt cagcaaaaac ctggcttcct cgtcggtttt
    57481 cgccgtcgtc acgatggtga tattcattcc tttgtttttc tcgattttat catagtcaat
    57541 ttcggggaaa ataatctgtt cctgcaggcc aatggaaaaa tttcccctgc cgtcaaacgc
    57601 atggggtgat atgccgcgga aatcccgaac ctttggcaaa gcagcgttca ccagacgatc
    57661 gaagaattca tacatgatct tttttcgaag cgtaacggaa cagccgaccg gcatgccttg
    57721 cctgagcttg aaggtcgcaa tggatttctt cgctttgttg acaatcggtt tctggccggt
    57781 tatggccgcc agttcctgta cggcaccgtc aataatcttg acgttctgaa tagcttcgcc
    57841 caaccccatg ttaatgacga ttttctccat cttgggcacc tgcataggat tcttgtactt
    57901 gaattcctgc accagcgcag gcgctacggt ctttgaataa tattctttta atcttgccat
    57961 tttatttaac ccacattaaa ttacgtcttt gcatttactg cagatacgaa ctttctttcc
    58021 atcttcaagt tttttacttt ggactctgac gggcttattg catttattgc aaaacaatcc
    58081 gacattggat acgtgaatgg atccttcttt ttccacgata ccgcctttgg agcgctggtc
    58141 cggtctttta tgtttcttga tgacgttcac tttctcgacc acgatcctgt ctttctcggg
    58201 aatgacggcc agaacttttc ccgtcttccc tttatcttta ccggcaagga ccttaaccgt
    58261 atcccctttt ttaaatttgt ttaaactcat ttttcccctc tgcaaaacat ttttacaaaa
    58321 cttcaggagc tagagatata attttcataa attgtctggc tcgcaattct cgggcgacag
    58381 gtccaaaaat gcgcgtcccg acgggttcca tctgattgga gattaaaacc gccgaattat
    58441 catcgaattt caaataagag ccgtccggtc ttctcacttc cttaaccgtg cggacaataa
    58501 cggccttgat cacatcacct tttttcactt tggaattggg aatcgcttcc ttgacggaaa
    58561 ccacaatcac gtctcccaaa ctcgcatacc gccgtttcga tcctcccagc accttgatac
    58621 aggcaacttt cttggcgccg gaattatccg caacatttaa tatggtctgc atctgaatca
    58681 tataaaaatc ctcatcaaac tgacttattc catcaggtca aacaatctat ttggctttct
    58741 ccaaaatttc cagcattcgc cagcgttttt ctctgctcaa cggcctggac tcaataatta
    58801 aaaccgtatc tccgattttg ctctggttgc gctcgtcatg cgccttgtac ttgacatgtt
    58861 tacgtacata cttgtgaaaa acaggatgct ttacaaaacg ttccgctctc accacaattg
    58921 ttttatccat tttattgctg acgaccacgc ccttaatggt gcgattaaat cctctttgat
    58981 ccacgttaac tcgctgcctc cttttctttc aacaccgtgt tgacacgggc aatatccttc
    59041 cggattttgc tcagcatgac ggttgattcc aattgccccg atgcatgccg gatcttcaac
    59101 cgaaaaagtt cttcactcaa ttcagcgatt ttttgtttga gcccgtttga gtcaagagct
    59161 cttatttcct taattttcat caactatatc cctcgataaa aattttgttg cgatggataa
    59221 tttatgggct gccagccgaa aagcctcgcg ggccacatct ttcgaaacgc cttccatttc
    59281 atagagaacg gaaccgggtt tcacgacggc cacccatcct tccggggcgc ctttcccctt
    59341 gcccatacgg gtttcagcgg gcttttttgt tacggactta tgaggaaata ctctgatcca
    59401 gattctgccg ccacgtttga cgtgacgagt catggcaaca cgagcagcct caatttgacg
    59461 ggacgtaatc caaccgcact ccgccgcctg caatccgtat tcgccaaaag cgatggaact
    59521 gccccttgtc gccttgccgc ccatccggcc tttctgcaac tttctgtatt ttaccctttt
    59581 cggcattaac ataaaaacaa actcccgttg tttctgacaa catccaaccc tgtccgcaca
    59641 aaccgttcag cgcccaggat ggacatcaat ttatttttca ttcttcgtcg gtaaaacttc
    59701 tccgtggaaa ataaaaactt ttacgccgat caatccgtaa gccgttttgg cttccgtaaa
    59761 tccgtaatca atatccgccc gcagcgtatg cagcggcacc cggccttcgc ggtaccattc
    59821 cgatcgcgac atttccgcgc cgcccagtct tcccgaacag gcgattttga tgcccttggc
    59881 gccaaacttc agggcatacc cgacgcactt cttcattgcc cggcggaaag caaccctttt
    59941 ctccagttgc atggccacgt tttccgccac cagttgcgca tcgacttccg gcttgcggac
    60001 ttcgagaata ttaatgataa tttccgactg cgaaaatctt tcaatttctg atttgagctt
    60061 ctcgatctcc gatccttttt tcccaatgat cactccgggt cgcgccgagt aaatattgac
    60121 tttcgctttg ttggctgctc tctcaatttc aattttggat agacccgcat gatacagttt
    60181 ctttttcaga tattttctga tctgcagatc ttcatgcagc acgtcgctgt attttttatc
    60241 ggtataccag accgattgcc attttttgat gtagcccaac cgtaatccta ccggattaac
    60301 cttctgcccc aagctgaacc tccttaaatt tcgtccaaaa taacggtaat atgtgaactt
    60361 cgtttcttga tgcctgccgc tcttccctgc gctctggcgc gccagcgttt caaggcgggc
    60421 ccttgatcga cataaatttc tttgacgtaa agaatatttt catcaatatt gggattctgg
    60481 gcagcattgg ccacagctga tttcagcacg ttggccacga cacctgccgc atatttcctg
    60541 gtgtagagaa gaatgttacg cgcttcctga acgtttttcc cccggatcaa atccgccaca
    60601 agccgaactt tctgcggcga cattctcata tattttgcaa cagcctttgc ttccatattt
    60661 atcaactcca agctttaccg ctttatctcc cgaaaatact agctgacttt ggttttccga
    60721 tcaccggaat gagaaaagaa aaccctggtc ggtgcaaact ctccgagttt atgccccacc
    60781 atattctccg tgacataaac cgggatgaat ttttttccat tatgtacggc aaaagtataa
    60841 ccgatcagtt ccggtgtaat agtggaacgc cgcgaccaag tcttgatgac attcttggtt
    60901 ccttcggcct ccatttttct gattttagcc agtaactttt cttcaatata cggacctttt
    60961 ttgatcgaac gcgccacgct tatttacctc gtctcttaat aatatactta tcggttctct
    61021 tgttctttct ggtcttatgg ccttttgtgg gaatacccca gggcgtgcac ggatgacgcc
    61081 cgccggagga tttgccttcg ccgccgccca tcggatgatc caccggattc atgaccacgc
    61141 cgcgcacatg aggtttttta cccagccagc gtgtccttcc ggctttgccg atgctgatgt
    61201 tttcatgttc gttattcccg acctgtccga tggtcgccat acattcaata aagattttcc
    61261 ggacttcacc ggacggcagt cgtacctgag cgtaacttcc ctctttggcc atcagctgtc
    61321 cataggcccc tgcagatctg atcagttggc caccccgtcc gactttcaat tccacgttat
    61381 gaatcaagga ccccagcgga atatacttca agggcacggc gttgcccggt ttgatatccg
    61441 ctttttcacc gctgtacagg acatcgccga cgttgacctg cgcgggtgcg acaatgtatc
    61501 ttttttcacc gtcacgataa ttgagcaggg cgattcgagc cgttctgttt ggatcatatt
    61561 cgatcgaagc caccttggcc ggaatgtcgg ctttattgcg gcaaaaatca atcacccggt
    61621 atttccgttt gtgaccgccg ccgatatgcc gggctgtgac tcttccgtaa ctgttacggc
    61681 cgcctttctt cttcaaaggt tttaaaagcc ttttcaccgg ctccgtagag gtaatttcgg
    61741 caaaatcgga aacgctctga aatcgtctac ccggtgatgt cggtttatac tttataattg
    61801 ccatccgtct aatccttcaa tgcattcttt cttatacgcc ttcagcaaat tcaatgctgt
    61861 tgccggcggc taatgtcact acggcctttt tccaggagct cttttgaccg actgtccttc
    61921 ccattctctt cttttttccc agaacatgca tcacgcgcac atccattact ttgaccttga
    61981 aaagcttttc aaccgcatcg gcaatttcca ctttgttggc tttgcgatct acttcaaaaa
    62041 agtatttatt ggatgcatcg cgcgccatgg tgcttttttc ggtaaccaat attctcttca
    62101 cgactttgct tctgtccatt ataccagcaa tgccccctca atctttttta cggagggttc
    62161 aagcagaatc aagtgttcat gattcaacac atcataaaca ttaataccct ctgatctgat
    62221 catcttgaca tttttaatat ttcgggaaga tttcatcaaa atctcattgt tatcatccag
    62281 cacaaataac gcttttttga gatcgaaaag atcgacgact ttctgaaatc cgcgcgcgct
    62341 gatcttctcc atcgggaaat ctttcaggat gatcatttta ttttctttga atttcaggct
    62401 caacgccgag atcagtgctt tcttgcgaat ttttttggga acgctgaagg aataatcccg
    62461 cggatgcggc ccgaaaacgg ttccgcctgt tctccagacg ggcgaccggg acgatcctgc
    62521 ccttgcacgg ccggttcctt tctggcgaaa cggcttttta ccgccgccgc tcacgtcact
    62581 cctggttttc gtcgaggccg taccggagcg tcgtgaggcc aactgcattt tgacgacgtc
    62641 gtatattacc gcctcattga cgtcggcccc gaagacggca tcattcaaat ccacctcggc
    62701 aactttcttt ttttcgatat ttaaaacatc tgcaactgcc atgtgcgtaa ctccagcctt
    62761 agcgtaatcg ctattttttc tttgcctttg taatcagtaa aaaactgttt ctacttcccg
    62821 gaacggaccc ctccagcaaa agcagattcc tttcggaacg aacctgccat accttgagat
    62881 tctgaacggt tttacggaca ttccccatgt gtccccccat cttggttcct ttgaaaacac
    62941 gggagggatc agcactggca ccaatcgagc ctggtgcacg atgaaacatg gatccatgcg
    63001 ttgccctgcc gccaccgaaa ccgtgtcttt tcacaactcc ctgaaaacct ttcccctttg
    63061 atgttccgac aacgtcgaca taatccccca tggcaaatat gtccgcagaa atttcctgcc
    63121 ccacttcgta cgtttcggaa tgcgccggga actctctgac aaaacggaaa aatcccttgt
    63181 tggccttatt aaaatgacct tgcagcggcc tggtcacatt tttctgtttc acacggccgt
    63241 aacccaattg gacggcatca tagccatcct tgtctttggt cttcacctgt aaaaccacag
    63301 agggttccac ctcgatggcg gttacaccga cggcagatcc gtcatccgca aatacttggg
    63361 tcattcccag ttttttacca ataattccct tgctcatctg acattccaat ttttaagtaa
    63421 taatatattc catttgaaag acggcattac cctatgccat ttttcatcat tttaaaaaag
    63481 cacgcctacg cttttatttc cacatccacc ccggctgaaa gatccagttt cattaaagcg
    63541 tcaacggtgg cttgagtagg cccgacgata tcaaccaatc ttttgtgggt tctgatttca
    63601 aactgctcgc gagatttttt gtccacatga ggtgagcgat tcacgcagta cttattgatt
    63661 tccgtaggaa taggaatagg accggcgaca cgtgcgccgg tctttttcgc cgtttccaca
    63721 atttcctcga cggaacgatc caacagttta taatcgtaag ctttaagccg aattctaatc
    63781 ttttgttctt tcatgggtta gctaacctat tttctatgca ataaccttgc ttacgactcc
    63841 ggcgcctacg gttctgccgc cttcacgaat agcgaatctc agctgttctt ccattgcaat
    63901 gggggtaatt aattcaattg acattttgac gttgtcgccg ggcatcacca tttccacgcc
    63961 ctcgggaagc gttgcaacac ccgtcacgtc cgtcgtccgg aagtagaact ggggacgata
    64021 accggagaag aacggggtat gacgaccgcc ttcttccttg gtcagaacat agacagccgc
    64081 ttcaaatttg gtatgcggtg taatggaggc gggcttggcg acaacctgtc ctcgttcaac
    64141 ttcttcccgc ttgattccgc ggagcaacaa gccgacgtcg tcaccggcac gaccttcatc
    64201 cagcgttttg cgaaacattt ccacaccggt cacaaccgtt ttaatggtct ctctgattcc
    64261 gatgatttcc acttcttcac cgaccttgac aataccgcgg tctactcttc cggtcacaac
    64321 cgtaccgcgg ccggaaatgg tgaaaacgtc accgaccggc atcagaaacg gtttgtccgt
    64381 atcgcgcttc ggttccggta cataattatc cacggcatcc atcagttccg caatgcattt
    64441 cacatcaggg gaattgatat cgtcggcctc caaggctttc agtgccgaac cgcgaatgat
    64501 aggaatattg tcaccgggaa attcatactg ggtcaaaagt tcacgcaatt caagttcgac
    64561 aaggtccaac agctcgggat catcaaccag atccacttta ttcagaaaaa cgacaatgta
    64621 aggaacctgc acctggcgag caaggaggat atgctcgcgt gtctgaggca tcggaccatc
    64681 ggaagccgcc actaccagaa tggtcccatc catatgggcg gcgccggaaa tcatattttt
    64741 gatatagtcg gcatggccgg ggcaatccac atgagcgtaa tgtctttttt ccgtctcata
    64801 ttccacgtgg gaaatattga tggtaacgcc gcgttccttt tcttccggag cattatcgat
    64861 ggaatcaaaa gctctgaatt ctgcaaaacc cttttttgcc agatatttgg taatcgcggc
    64921 cgtcaatgtg gttttaccat gatccacgtg accgatcgtt cctatattta aatgcggctt
    64981 agtcctttca aattttttct ttgccatttc acctttcctc cttaactttg aatggttggt
    65041 ttccttatcg ttaaaattat gatttaaaat ttataatgag caaaggcttt gttcgcctcg
    65101 gccattttat gcacagcttc cttcttctta atcgctcccc cgcggttgtt ggcggcatcg
    65161 atcaattccg aagccagttt ttcccgcatg gttttttccg tacgttcctg ggcattggat
    65221 atcaaccagc gaatcgccag cgcggtgcgt cttgccggaa caacttccgt cggcacctga
    65281 taggtagaac cgccgacacg cctggatttg acttcgatca ccggtttcac attgttgaca
    65341 gccttttcaa aaaaatccac cggttgttcc ttgacccgct tttcaataat atcaaaagcg
    65401 ccgtagagaa tgctttctgc aacgctcttc ttacctcttt tcaacagcga attaataaat
    65461 tttgcgacca gtttattatt aaacttggga tcgggcaaaa tatctctctt ttcaacttcc
    65521 cgtcttctcg gcattgaatc ctctccgttt aacttggctt tttagcaccg tacttcgatc
    65581 gaccctgttt gcgatcctga acgccgaccg tgtccagagt tcctctgatg acatgatacc
    65641 ggacaccggg cagatccttg acccttcccc cgcggaccag aaccactgaa tgctcctgca
    65701 gattatgacc gatacccgga atgtatgacg taacttcata accgctggtc aatcgcactc
    65761 tggccacttt ccttaaagcc gaattcggct tcttcggtgt cgttgtatac actctaacac
    65821 aaacaccgcg acgttgcggc gaacccgcca gcgcgggtga atttgttttt ctcttaatct
    65881 ttgctctgcc cttgcgaacc agttgactaa ttgtcggcat tttcctccta aaacaaatcg
    65941 ctgctcttca atgtattata accggcagcg aaccggtggt acgtaccagt tcaaattact
    66001 aatgtcaagc attttctgcc gatattttaa tttattctgc cgccgtctcg gcattctcat
    66061 tttccagcgc aattcccaac ttccggtaca tgaccaaacc ggttccggcg gtaatcaacc
    66121 ggcccataat gacgttttcc ttaagccccc ttaaataatc cgttttaccg gaaagggcgg
    66181 cttccgtcag gacctttgtg gtctcctgga aggacgctgc cgaaataaag ctctgggtgg
    66241 aaagcgatgc tttggtaatc cccagaagca tcggctcgcc gatagcgggt cggccgccct
    66301 gggcaataat cttttcgttt tcttcctgga agcggtatcg ttcgattttt tcgtcggcga
    66361 taaatgcggt atctcccgga tccattacct taacgcgtct gagcatctgg cgaacaatga
    66421 cttccatgtg tttatcattg attttcacgc cctgcaaacg gtagacttcc tgaacttcat
    66481 ccaccagata acgggccagt tctttttcgc ccttaatgga cagcagatcg tgaggattgt
    66541 tgacaccgga catcagggca tcaccgggag aaacaacatc gccctcctga acgctgatat
    66601 gtttcccttt gggaataaga tactcttttt cctcgcccac ttccggggta acaacgatct
    66661 tacgtttccc tttggcatct ttcccgtagg aaaccacacc gctgatttca ctgatcacgg
    66721 cgaaatcttt gggtttacgc gcctcgaaca gttcggcaac gcggggcaaa ccacccgtga
    66781 tatctttcgt ttttgtcgtt tcccggggaa ttttcgctat aatatcaccg gctctcacct
    66841 gatctccttc agaaaccaca aggttgaccc ctactgagag cagataccgt gcaacggcat
    66901 tggaatccgc cagtttggct gtttttcctt tttcgtcctt aatggaaaca cggggtctca
    66961 aatccccgcc cttaaactca acgatgatct ttcgtgaaag accggttact tcatcaacct
    67021 gttcctgcat ggtctttcct tcggtgatat catcaaactt gatcgttccg tccacttccg
    67081 ccagaatcgg tatggagaag ggatcccatt cggcaatcaa ctgtcctttt ttcacttcgc
    67141 tgttatccgt aaccttcagg tgggcgccgt acgacaggac gtacttgccg cgaacgtgtt
    67201 tctgttcatc cagcacgtgg acttcgccgt tgcgtttcat cacgaccagg tccccttcct
    67261 ttgtttttac cgtgctgaga tttacaaatt tcacaattcc gtcgtgacgg gcttccagag
    67321 tcgaatgttc gtcaaatttt gccgtaccgc cgatatgaaa cgttctcatg gtcaactggg
    67381 tccccggttc gccgatggac tgagcggcaa caataccgac cgcttcaccg atattaacga
    67441 ggtggccgtg cgccaggtca cgtccataac acatcgcaca aaccccgtgc ttggaacgac
    67501 aggtcagaac cgaccgaata tggacttttt cgataccggc attgtcgatc ttttcagcca
    67561 gcgtttcatc aatcatctga ttggccggca cgataacttc tccgctaaac ggatctttaa
    67621 catcctgcag cgcaactctt cccagaacac gttctccggc ggtttcaatg atttcgccgc
    67681 cttccatcag agctgataca tacacaccat ccactgtttc gcagtcatgc tccgtgatga
    67741 tgcagtcctg cgccacatct acgagtcttc gtgtcaggta accagagttg gccgtcttca
    67801 gcgccgtatc ggccaatccc ttacgggcgc cgtgcgttga aataaagtac tgaagaaccg
    67861 tcaatccctc gcgaaaattc gctgtaatag gcgtttcgat aatttcaccg gagggcttcg
    67921 ccatcagacc gcgcataccg gccagctgtc ttaactgctg tccggagccg cgggcacccg
    67981 aatcagccat catgtaaatg ggattgaaac tctcgatttt gcgtttcttt ccctcggcgg
    68041 ccgggacatc ttcggtactg atgcctttga gcatttcgga tgctattttt tcagtggcct
    68101 gtgcccaaat gtccacaact ttgttgtaac gttctccgtc ggtaatcaag ccgtccatgt
    68161 actgtctttg aatcttcaaa acatctttat cggcctgcag gacaattttt ttcttttgct
    68221 ccggaatgat catattgccc acggcaaagg agagccccga aacggtcgca tacttgaacc
    68281 ccagatcctt gagtttatcg gccagaatga tggttgtctt gtcgccgcac agacgataac
    68341 aatgatctat caggctggtc agttctttct tgttcatcag tttgttgata tcgtcgaagc
    68401 tgatctgctc gggcacgatt tcggaaagaa tgattcgtcc agccgtggtt tctttcagtt
    68461 ccgaatcaat gcgcaccttg atccgcgcat gcaggtcaat cacgccgtta tcaatggcgg
    68521 cgcgcacttc gtcgggaccc gcaaaaatca tcccgtcgcc cttgacgccg gtcttggccc
    68581 gggtcaggta ataaataccc agaacgatgt cctggctggg aatgatgatc gggctgccgt
    68641 tggccggaga aagaatatta ttggtggaca tcatcagaac gcgtgcttcc gtctgtgctt
    68701 caacggacag cggaatatgc acagccatct ggtcgccgtc aaaatccgcg ttgaacgccg
    68761 tacaaaccaa cgggtgaagc tggatggcct tgccttcgat caggaccggt tcaaaggcct
    68821 ggatgcccag acgatgcagc gtcggcgcgc ggtttaagat gatcggatac tcgcgcacga
    68881 cttcatccag cgcatcccag acttccgccg attccttttc caccattttt ttggcacttt
    68941 tcaccgtggt tacatatcct ttttccagta accgattgta gataaaaggc ttgaaaagct
    69001 cgatggccat tttcttcggc aaaccgcact gatgcagtct cagatccggg ccgaccgtaa
    69061 ccacggaacg tccggagtag tcgacgcgct tgcccagcag gttctgacga aaacggccct
    69121 gcttgccttt gagcatgtcg gacagggagc gcaacggcct tttgttggtt ccggtaatcg
    69181 cacggccgcg tcggccgttg tcaaaaagaa cgtccaccgc ttcctgcagc attctctttt
    69241 cattgcgaat gatgatttcg ggagcgttga gttcctgcaa tctcttcaga cgattattcc
    69301 ggttaatgac gcggcgatat aaatcgttga gatccgatgt ggcaaaacgt ccgccgtcca
    69361 gtggaaccag cggccgcaga tcgggcggaa tcaccggcag caccgtgaga atcatccatt
    69421 cgggcttatt gccggatttg cgcagcgctt caacaacctt taagcgcttc accagcttct
    69481 ttttcttggt atcggaaacg gaggtcacga tttccgtacg cagttcttcg tagagttttt
    69541 ccagatcaat ttcttccagc agttgtctga tggcctcggc gccgataccg gcgacaaaac
    69601 cgtcattacc gtactgttcc ctggcctctt cgtattcctc atccgaaagc agatcttttt
    69661 tcttcaatgg cgtattcttg gggtcaagaa caatccatga ttcaaagtaa agaactttct
    69721 ccaattcttt aagagaaagg tccaccgcgt tgccgatgcg gctgggaaga cttttcaaaa
    69781 accagatatg agcgacgggc gtcgcgagcg ttatgtggcc catgcgctcg cggcgaactt
    69841 tggactgaat gacctcaacc ccgcactttt cacagaccac gccgcggtgc ttcattctct
    69901 tataacgtcc gcaaagacat tcgtaatcct tgaccggacc gaaaatcttc gcgcagaaga
    69961 gaccgtcttt ttccggctta aatgttcggt aattgatggt ttccggtttt ttcacctcac
    70021 catgggacca ggacagaatt ttatccggtg aagcaatgga aatctgcatt gcgttgaatt
    70081 taaccaaatt cttcggtttc tcaaaatagc tgaaaacgtc ttccacaaat tttctcctgt
    70141 acgtaaataa cggtgtgaaa ttattaagcc gcctgttgtt gctttttttg ccgcgatacc
    70201 cctaggctaa cttaggctat ccgactcttc gactaattcc acatccaatc caagcccctg
    70261 aagctccttc accaaaacgt tgaaggactc cggcaggccc gactcaaaat tatgttcacc
    70321 cttgacaatg gattcgtata ttcttgttct gcccgccaca tcatccgatt tgacggtcag
    70381 aaattcctgt aaagcatatg cggcgccata agcctccatg gcccagactt ccatttcgcc
    70441 cagtcgctgg ccgccgaact gcgccttgcc gcccagcggt tgctgggtca ccagcgaata
    70501 gggcccgatg gagcgcgcgt ggatcttgtt gtccaccagg tggtgcagct tcatcatgta
    70561 aataatgcct accgtcacct cctgatcaaa gggctcgccg gttctgccgt caaaaagaat
    70621 ggactggccg gtctccggca aatcggttct tttcaacatc tctctgattt gatcttccgg
    70681 acatccgtca aaaaccggac tggcaaccaa catgcctttc ttcaaacggc cgacaaatcg
    70741 tttgatatct tccgtggacg cctgatcgat aaatttgtca aaatcgggcg aagagtaaac
    70801 ctttttcaac tcgtctttga tatgactcaa accggcgcgc tgctgttcca gcagttcatt
    70861 caaaccctca ccgatcgatt ttgccgccca gcccagatgc gtttccagaa tctgccccac
    70921 gttcattcgc gaaggaacac ccagcggatt taagacgatg tccactgtgg aaccgtcggc
    70981 aaaatacggc atgtcttctt ccggcaggat ccgtgataaa acgcctttgt tcccatggcg
    71041 ccctgccatc ttgtcgccca cggacagcct tcttttaata gcaatataca ccttgaccat
    71101 cttgattaca ccgggcggaa gttcatcact ggccttgatc ttttccatct tctcctcaaa
    71161 atgagcactg acaaaatcga tctttcgttc cagtgaagcg atcagaccgg cgatcttttc
    71221 ttccgaaccg tcgttctcgg cgatggtaat atctttccag ctggagaaag gcagatcgtt
    71281 ccagatctct ttcgtaattt tgtcgccctt cttcagaacg gttttctttt tggcatcccc
    71341 aattctggtt gccgccgttt tgccgacaac aagtttggta atatcttttt tgatggtttc
    71401 ggtgagaatg cggatttcat catctctgtc cttcgcgatc tgctcaatgg catccgcctc
    71461 aatggcctgt gaacgggaat cgctttccgc gccttttctg gtaaaaattt tggcgtcaat
    71521 aaccgttcct tccacgcccg gcggaacgcg caacgatgta tcccggacat cgctggcttt
    71581 ctctccgaaa atcgcccgca gaagtttttc ctcagcggac agctgtgttt cgcccttggg
    71641 cgttattttg ccgaccagaa tatccccagg tttgaccgaa acgccaatgc aaacaatacc
    71701 gctttcatcc aggtttttga gaacctcttc gccgacgttg gggatatcgc gcgtaatttc
    71761 ctctttgccc agtttcgtgt cgcgggacat ggtctcgaat tcctcgatat gaatcgacgt
    71821 gaaaatatct tccttgacga ccctctcgct gaccagaatg gagtcttcat agttgtaccc
    71881 gccccaggac atgaaagcga ccattacatt tctacccagt gccagttcgc cgttatcaat
    71941 ggccggtccg tccgcgagga tatccttttt atggacacgc tgcccgacgc tgatgatcgg
    72001 tttttgattg aaacaggtat cctgattcga tcgctggtat ttcgccagat tataaatatc
    72061 cacaccgctg tcgatgccgt cttcatccat ccggtcggcc cgcacaataa tgcgtgaagc
    72121 atcaacgctt tcgacaacac cggaccgttt ggccaggacc acagcgccgg aatcgcgggc
    72181 taccacggat tccacgcctg tccccacaac cggaacttcc ggattcatca ggggaaccgc
    72241 ctgccgttgc atattcgatc ccatcaacgc acggttggcg tcatcatgtt ccagaaaagg
    72301 aatcaacgct gcagctacgc tgaccagctg ggtgggcgaa acgtccatca tcttaatctc
    72361 ttccggctca accgaaatat agctgccgcc ttttctgacc gaaatcaatt cttcggcaaa
    72421 ttttccgttt ttactcagag gacggtccgc cgaagcaata atctgatttt cttcctcaat
    72481 cgccgttaca aagtgaacat catcggacac ccggccgttc tccactttcc ggtacggtgt
    72541 ctcaataaaa ccgaattcat tcactctggc ataggtgctc aacgacacga tcaatccgat
    72601 gttcggacct tccggtgttt caatcggaca aatgcgtcca taatgtgtcg gatgaacatc
    72661 gcggacttca aatcccgcgc gttcacgggt caagcctcct ggacccagag ccgacagacg
    72721 tcgcttatgg gtaatctccg aaagcggatt ggtctgatcc atgaactgag acaactggct
    72781 ggaattgaag aattcattga ccaccgcaga aaccggtttg gcattgatca ggtcgtgcgg
    72841 catcatggtc tcgatgtcct gaagactcat tttttccttg atggctcttt ccatacgcac
    72901 cagaccgatg cggtactggt tttcaatcaa ttcgcctacc gaacgcaccc ggcggtttcc
    72961 caggtgatca atatcgtcca cactgcaatc gccgacgccg tttttcaggt cgatcagata
    73021 tttcactgcg gacaggatgt cttcgttgct caacacggtg ttgtccgtcg gcacgttcat
    73081 gtgcaatttg taattcattt tcgcgcgtcc gacattggac agatcataag tctccggctc
    73141 aaagaacagg ctcttgaaga acttgtccgc tgtttccggc gtaggaggat tgctcggacg
    73201 caaacggcga tagatctcca tgatcgcatc ctcggtggta tcaatcttat ccagcagcag
    73261 cgtatcccgg atggaagcgt tatccatgtc ttcatcaata tgaataagtt tcaattcccg
    73321 gatattcttt tcgcggataa tgtccagaca gttcgccggc agttcctcgt tgcaacgaaa
    73381 aacaatctca cccgtttccg gatcatgaac atcccgagaa ggaatttttc cgatcagttc
    73441 gttttcattg attgccgtcc gtttaacacc ggcctccatg attctcttga tcagcggctt
    73501 ggtcagtttt cttcccttct tgacaatcag ttcggaactc ttggagtctt tgatatcttc
    73561 tggtgatttc ttgccgacca gcgattcatc aacggcaaaa taaatctttt tattgtcatt
    73621 gatcactgtg ttgacgttat aaaaataatt caggatgaat tccgtcgaat tccccagcgc
    73681 tttcagcagg atggttaccg gcatttttct gcgacggtcg atccggacat aaagaatatc
    73741 tttggaatca aactccagat ccagccacga tcctctgatg gggatgactc ttgcggaata
    73801 aatcagttta ccgctggcaa caattttacc tttgtcgtga tcaaaaaaga tacccggcga
    73861 acggtgcagc tgattgacga tcacccgttc cgtcccgtta atgatgaagg taccgttttc
    73921 cgtcatcaac ggaatttcgc cgaagtaaac ttcctgttcc ttgatatcgc ggatctgctt
    73981 ggcttcggtc gtttcaccgg cacctttccc ttcggcgggg cgatcggcct caaacacaac
    74041 caaccggacc acgatcttca agggagccgc gtaggtcatt cctttttgaa cacattcttt
    74101 cacatcatac cggatatcac ccagtttata gctcacaaat tccagagaac atttcccgct
    74161 gaaatccgaa ataggaaaaa cgctcttgaa agcaccctgc aaaccgaaag cgccccgctt
    74221 caccggtgca atatccttct ggagaaattt ctcgtaagac tctgtctgaa tatcgattaa
    74281 attggggata tccagtatct ttcttacctg accaaaattt tttctaaagt ctcccatggc
    74341 atccttaaaa atgaataata tatgtttgag attgcgttat ttaatctcga cggtaccgcc
    74401 cacttcttcg attttcttct tgatgtccgc agcttcatcc ttggacgcac cttccttgat
    74461 gggcttggga acgccttcca ccagatcctt ggcttctttg agtcccaggt tggtaatggc
    74521 acggacaacc ttgatgacct gaatcttctc cgcgccaaag ccggtcagaa tgacatcgaa
    74581 ttctgttttt tcttcaaccg gacctgcagc agcggcggca cccggggcag ccacggcggc
    74641 cataaccgga gcggcagcgg aaacaccaaa tttatcctcc agctctttga tcagagcgga
    74701 aagttccaat accgtcattt cttcaataaa tttgaccaca tcttctttcg taattttatc
    74761 tgacatgttt tattcctttc tgtatgttgt attagtttaa agattttttc ttatcgcaat
    74821 aagcgttaag cacttgcacg aaacttcccg gtactccatt taaaactctc acgaaggatg
    74881 tcggaacagc cgccatgacc gaaaccagtt tgcccagaag gacgtctttg ccgggcaatt
    74941 ctgccagaac cgtcaggtcg ttcctggtta aaagcttttt atccagcaca ccaaccttga
    75001 tttccagttc cgcgtttttc ttggcaaaat caatcagaac cttcgccggc gccaccggat
    75061 ctttgtagct caggacaatg gcaacgggcc atttgaaatg atcggcaagt atgctgaaat
    75121 cggtgccttc ggaagcaatg cgtaaaagtg tattctttac gaccttccat tcggcattgg
    75181 acttgcgcaa ttcatttctc aaagcttcca ttttttcgac attcatcccg ctaaagctgg
    75241 tgagaatacc taaattagct tctttgagtt tcttttggag ttcggataca atttgttctt
    75301 tcgttttccg atccaatcta tcagcctcct ttcaaaatta ttatattgct gaggccggag
    75361 aattaagcca catacagaac cccgataccg ggattgttcg acttacaaat ttcaatacac
    75421 ctatgaagcc ctgtagcgca ttcgtttttg aaatttgagt ctcacaacaa aaaaacggta
    75481 acccagccta catcccgatt cccgttttac aaaaaaagtc tacccttaca aatcaagtaa
    75541 caatttttat tttacgagtc tcggcaggcc ttttcaggtt taaacgtctg tacctgctgt
    75601 cttcgacctt gtaaccaact aaaaaatgcc cgatatccta agctgcttta atatcgagcg
    75661 gatcaattct caccccgaca cccatcgtgc tggagatcga aatgcttttg atatacgtac
    75721 ccttgcttga tgccggtttt aatttaagaa tggtctccag caaggcacta acattttctc
    75781 tcaatttttc agcaccgaag gatacctttc ccaccggaga atgaacaatg ccggcttttt
    75841 cgacccggaa ctcgacttta cccgctttga gttctttcac cgcattggcc acgtcaaacg
    75901 ttaccgtccc caccttgggg ttgggcatca aaccgcgagg tccgagaatt ttaccgattt
    75961 taccgactga tcccatcata tccggcgtgg cgataacccg atcaaattcc atccatccgc
    76021 ctttgatttt ctcgataact tcgtcattgc ccaccagatc ggcaccggcc tccaaagctt
    76081 ctttttcctt ttcacctttg gcaaatacca ggaccctgac tgttttaccc aatccgtgcg
    76141 gcagaaccac gctgccccgc accatttgat cggcgtgagc gggattaaca cccaaacgaa
    76201 tcgccgcatc cacggtctca tcaaatttcg ttcccgccgt cgatacaaca atttccgcag
    76261 cctctttcaa cgtgtatttc ttggccggct cgactttagc ctttgccgcc aatatacgtt
    76321 tgcctcttct taacatgatc aaacctcttt atttattaac ctgtaatttc tattcccatt
    76381 gaccttgccg tgccggcaat gatatttatc gcgccttcaa tattaacggc gtttaaatcc
    76441 ttatacttca gttcggcaat atcctttacc tgagatgcgg tcaccgaacc cactttatcc
    76501 cgtttgggat cgctggatcc cttggcaatt tttgctgcct gcttcagtaa caccgacaca
    76561 ggaggtgttt ttgtaataaa cgtaaaagag cgatcggcat aaaccgtaat cacaacagga
    76621 ataatcattc cttcctggct ctgcgtcatc gcgttataag ccttgcagaa ttccatgata
    76681 ttcacgccgt gctgacctaa tgccggaccg atcggaggcg aaggggttgc cttgcccgcc
    76741 ttgatctgta atttaatgtt cgcaataatt ttttttgcca tatcgaatca ccctgatctg
    76801 aattttaaat attatttatt cggcctatgc aaagcattac ccctgcgtca cctggatgaa
    76861 atccagctcc actggcgtcg gccggccaaa aatacttacg ataaccctaa gcttactttt
    76921 ttccggtttt aattcatcga taatgccgtc gaaattcgta aaaggaccgt cgataatttt
    76981 gacatgatcc ccgacatcga atttgaattt gggtttgggt tttaacgttc cttcatcaat
    77041 tctgatttta agatgttcaa cgtctttatc aggtatggga gaaggtttat ccttgctgcc
    77101 gatgaatcct gtcactttgg gagtatcctt gaccacgtgc caggcttcat cattcatatc
    77161 catccgcaca aggatatacc ccgggaaaaa cttgcgtttc gaactttttt tctctcccga
    77221 aatcaattcc acaacatctt cttcaggaat cagaatgtcg gaaaaatacg cctgagttcc
    77281 ggtcgtctta attctttctt caagacttat tttgacttta ttctcgtatc cggaataagt
    77341 atgtacaacg taccatttaa aagccatttt cctaacccaa tattaatttc actgctttag
    77401 cgagggcaaa atcaataatg cccagaatta aagaaacaat aaaaaccaga atgatcacca
    77461 ccgcggtaga cgccagggcc tgttttggag tcggccaggt aacttttttc aactccatct
    77521 tggcatcttt gagaaactgt gtaatctttt gaatcgttac ttttactttt tctttaaaat
    77581 cttccatctg actcatcctt cacacaacgg ggaaaaatgg caggccagga gggacttgaa
    77641 cccccagcat ccggatttgg agtccggcgc tc
//
LOCUS       DAOOLB010000002        61372 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_313_length_61372_cov_45.206974, whole genome shotgun sequence.
ACCESSION   DAOOLB010000002 DAOOLB010000000
VERSION     DAOOLB010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 61372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 61372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61372
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_313_length_61372_cov_45.206974"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="PLF77_00440"
     CDS             complement(<1..331)
                     /locus_tag="PLF77_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07349.1"
                     /translation="MKTKTVSPFIQKTSVKDWADWRWQIKNRLTHPLLPCAQPQTAAS
                     HRETLQSVTAVYPMAVTPYYLSLIQHQDFHDPIYAQCMPDPREISTAGRAAEDPLQED
                     LHMPVPKL"
     gene            482..1465
                     /locus_tag="PLF77_00445"
     CDS             482..1465
                     /locus_tag="PLF77_00445"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPC07350.1"
                     /translation="MQQRKENVAMLSVASNAFLVLFKVLVGFAIGSVSIISEAIHSGV
                     DLLAAVIAMFSVKTSSVPADERHPFGHGKIENLSGFVEALLIFGAAFWIIFEALKKLN
                     APQAMENVGWGVGVMLFSAVMNFIVSQKLFKVGKEADSIALQADAWHLRTDVYTSAGV
                     MFGLTIIWLGHKFFADPNIYWVDPVAAILVAALIMRVAYRLTVDALSGLVDVQLPREE
                     ENFIRSMISVEKAVYGFHQLRTRKAGHVRFVEFHIKVDPQMSVHDSHGITRILKKNIR
                     ARFPESTITIHIEPCDGECSDMCQAGCLLPREKRPDKGMIRPQPIGHDQSS"
     gene            complement(1477..2259)
                     /gene="truA"
                     /locus_tag="PLF77_00450"
     CDS             complement(1477..2259)
                     /gene="truA"
                     /locus_tag="PLF77_00450"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPC07351.1"
                     /translation="MASYKFKILLEYDGTGYRGWQTQKNARSVQETLIKAAQSFLDVP
                     VRIQGAGRTDAGVHALEQAAHLESPKKVHPDILRTGMNDHLPSSVNILRVENAPARFH
                     ARHHALSRSYLYLISRRRTAFGKRYVWWVKDRLDCDKMKQTLDVFQGFHDFASFADKR
                     MDKEASTKVNIEGLWLKEFDNIIAVRILGSHFLWKMVRRIVGVTVELGRGNLTRPDVE
                     KMMTSFSELPAHFTAPPSGLFLEKVLYEGDKLPPVTPPICLL"
     gene            complement(2341..3681)
                     /locus_tag="PLF77_00455"
     CDS             complement(2341..3681)
                     /locus_tag="PLF77_00455"
                     /inference="COORDINATES: protein motif:HMM:NF024314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family N-terminal domain-containing
                     protein"
                     /protein_id="HPC07352.1"
                     /translation="MKVKYVCVVLALFLVSCYLQPPDTLQDVKDVTDLPQDHTARLAD
                     VPADKVLLTRDSQTAMNEHYDKTFFSVWHHPEPVHALPERLSAIFTQFAAHPGYGENK
                     RKHSTAWLKKLQQNAWLENYPNTLQPAITVRSTNLRMLPTNGPHFYKPGGDINGWPFD
                     NLQISSVAANTPILICHISRDRSWALVETSFAFGWIPAEDFARVDDPFMQTWQTSRYA
                     TVTRDKIPIVDANGNFSLRTSIGQMFPLITEMADKLEILIASADANHQAIIQRVFISR
                     EIMNIKPLPFTYANATRIANELIDEPYGWGGLYGNRDCSAMTRDFFAPFGIWLPRHSA
                     DQAKKIGIYIDLEAMEPQEKEKVILEQGIPYLSLLWRRGHIMLYIGERNGRALIFHNV
                     WGLRITDARGEEGRKIIGKAVITTLHPGKELTNIDPDALLIKNLSAMTILAPAK"
     gene            3891..4439
                     /locus_tag="PLF77_00460"
     CDS             3891..4439
                     /locus_tag="PLF77_00460"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HPC07353.1"
                     /translation="MDSFVPKKIFFTKGVGTHKDELHSFERALRDAGIEKCNLVQVSS
                     IFPPRCKMISKTQGLKQLTPGAITYCVMSRCCTNEPKRLMAASVGCAIPADKSSYGYI
                     SEHHAFGQNEKQAGDYAEDLAAAMLASTLGIDFNVDESWDEKKEIFKISGKIVRTLNV
                     TQSTICKDNKYTTVIAAAVFVF"
     gene            4526..5404
                     /locus_tag="PLF77_00465"
     CDS             4526..5404
                     /locus_tag="PLF77_00465"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPC07354.1"
                     /translation="MTQTERILDIPSLNHHDIGGAHLPYLYYEGETPQILFAHATGFL
                     PWLWHPILEELTPPRRQAWAPYICNYRDCDPESGGLSWDVIARDLISFCSVQNISDNL
                     AVGHSMGATVVTIASALFGLQPRGMILIEPIFLPEDFYTIDIQLKDHPLASLSIRRKN
                     GWKDENAALEYLKSKSLFAGWDEQMLQFYLQYGMEKQAGGDLKLTCAPRNEAALFMGG
                     KSTNPWPLLEKLTCPVLILEGEKSSNREFIDLPKAASLLRDGRYQLIPDAGHLIPMEK
                     PKEIAKIIKEFLADVF"
     gene            5546..6331
                     /locus_tag="PLF77_00470"
     CDS             5546..6331
                     /locus_tag="PLF77_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC07355.1"
                     /translation="MSKPFALIGRNVIRWINSIGAATIFLVLALLKTFHPKQFQKVIQ
                     QIYYIGARSTTIIMLVSLFTGMVLGLQSYHALVQFGAEGSLGTLVSLSLIRELGPVLT
                     AIMITARAGSAITAEIGVQRISEQIDALETMRIDPFRYLVTPRITASIVSFPLLTTVF
                     DLIGIIGGYVSAVALMGLNAGVYSHSIRSYVDMEDLTNGFVKSLVFAVIVATVCCYQG
                     YFVHMREDSKGAKAVGLATTSAVVISCVLILVADYVVTSLLMV"
     gene            6339..7583
                     /locus_tag="PLF77_00475"
     CDS             6339..7583
                     /locus_tag="PLF77_00475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC07356.1"
                     /translation="MNTPLIEFKNITKRFGEQTVLDQVNLQIYEGEVTTIIGLSGSGK
                     SVLLKHIIGLLKPDEGTILFRGEPINDMKKTELAEAMSKISYMFQSNALFGSMTVYEN
                     IALPLLENTAMKKNEIHDRVMARIEQTELTEAAQKYPSELSGGMQKRAALARALITDP
                     QVVLFDEPTTGQDPVRKNAILSMIAQYQKKFNFTAVMVSHEIPDVYFISNRILALYDK
                     SIVFQGTPEELSNFDHPFNDEVIRSLEGLQKELTGLYSRRQFKVLHRSQLQSRKSDEG
                     YCVVVFDLSDMNSIVSAIGYDRAQETIHSMGKYIDKHFDAIGGFSTRRKINEFVTVLP
                     FSDIAETESIVKDFMEDFQKSGMSEIWSEAQKNDPQTRCVDFSVKAGMAEGTPVAEID
                     SIVKLAKAHRKEIGRLQCAVKE"
     assembly_gap    7620..7719
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7722..8198
                     /gene="mlaD"
                     /locus_tag="PLF77_00480"
     CDS             7722..8198
                     /gene="mlaD"
                     /locus_tag="PLF77_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HPC07357.1"
                     /translation="MPSRSKHMKKYEIETVVGIFVFLGLLCIGYMTVKLGNVSFLEAA
                     SYPLEAKFTSVNGLRIDSSVKVMGLTVGKVKQIAMDQDNQRAVVKLNIQKGIKIYDDA
                     IASIKTEGLMGDKYVSIDPGGGGSLLEPGGIILETQSPLDIETLISKYAFGDVKKQ"
     gene            8233..8829
                     /locus_tag="PLF77_00485"
     CDS             8233..8829
                     /locus_tag="PLF77_00485"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPC07358.1"
                     /translation="MKKLFTQTAIILVIVMFSLPVYAGAALDSVQKCVNEVIGIVGDP
                     KLKSESAKELKKEKLRVLYKQMFDEIEFSKRTLTRNWNNFTPAQRTEFVNLFEQVLET
                     AYGDKVLSYTNEKVVFYKETTLSATQAEVQSKIVTSSKEIPVFYRVILKDGKWKVYDV
                     VVENVSLVQNYRTQFNDILSKDKPEKLLEVLRNKVKQH"
     gene            8897..9826
                     /locus_tag="PLF77_00490"
     CDS             8897..9826
                     /locus_tag="PLF77_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HPC07359.1"
                     /translation="MKSRLVFLVSFIWMLSGGAPGSAHAFVDLPPEQTSPPPVVLTVS
                     PDRPANQFAAAAVVDPMEEEYTGDEFDDYAGDNAKEEKVSIADPLEPFNRAMHEFNDK
                     MYFWAVKPVAQGYKAVVPEPARISVDNFFSNLGFPARFLSCLLQADFKGAATEAGRFT
                     INTVWGIGGLLDPSSGKELNLQEQNTDLGQTLGVWGVGQGFYIVWPVFGPSTPRDTVS
                     IAGNYFLDPVAYLNPWSARLGVNVLDKVNAASLRIGDYEALKNAAIDPYVAFRDAYIQ
                     YRYKQIKARKAKIFSGSSAAEPFPETVRPDKVD"
     gene            9827..10564
                     /locus_tag="PLF77_00495"
     CDS             9827..10564
                     /locus_tag="PLF77_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07360.1"
                     /translation="MKKIRCAPYWMVFVLTALVFFTTGPAASAAGLFSAFETVSRQDG
                     GLNTAIGYRYHQDTYEKGNGLKIRQNEIYSQAAYGADNAWEIYARLGIADLKIGDVFR
                     ESEEVFATLGGKVFYPVSGKLGVGAFVQGTYYLNDFSDTVERRKHVQLKDLWDVHFGV
                     ALQATLPCGSTMYAGPYLYYSEGTLSLSSNVSDWGYAAGRYSVQNTSIAGGFAGLDIP
                     LAKGFRLNVEGQYADRFSAGAAISYTY"
     gene            10622..11338
                     /locus_tag="PLF77_00500"
     CDS             10622..11338
                     /locus_tag="PLF77_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC07361.1"
                     /translation="MNQQKEKFFQVQDLTKVYQMGEVAVHALRGVTMELYAGELVVLL
                     GPSGSGKSTLLNIMGGLDTATGGVVSYRGKELTKATEKELTEYRRFHVGFVFQFYNLI
                     SSLTARENVSVVTEIAPDPMAPQQALDLVGLSDRLNHFPAQLSGGEQQRVAIARAISK
                     KPAVLLCDEPTGALDSQTGIVVLQALERINRELGTATAIITHNVDIAGMADRVIHLSN
                     GHIASVEINKVKKSASELQW"
     gene            11340..13706
                     /locus_tag="PLF77_00505"
     CDS             11340..13706
                     /locus_tag="PLF77_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPC07362.1"
                     /translation="MMKTLNRKLWRELWLMKGQVFAITLVVMSGVATFIMFISTMHSL
                     DFTRSKFYREYNFADVFVNLKRAPETLKYKINDIQGVHQVETRVCAQAKLDIRGFKES
                     VTARITSIPDDGDPLLNRLYIRKGRLPDPARDNEVAMNETFAQAHGLNPGDKFAAVIN
                     GKWKRLTVTGIVLSPEFILLMKTGAMTPDFKRYGALWMTRKAISKAYDMEGAFNDVVL
                     TVYPGTALSDVVRDLDNILGRYGGFGAYGRKDQISHRLLSEEFRQLKTSSRIYPTIFI
                     CVAAFLLNIVMSRTISTQREQIGTLKAFGYNNLSIAVHYATMVMMIIATGVLLGIAGG
                     IWFGKMLGALYMETYRFPFFHYILHPQVMIAVVVISVAAALSGTMHSLWNAARQTPAE
                     AMRPEAPVKYRVSLIEKTGIGRWLSQPSRIIFRNIERKPVKTVLAIIGIATACATMIC
                     SGFFRDCIDYMVHIQFVLSQKEDMTVSFVEPTSYRAVYELKRMEGISSGEAFRSVPVR
                     MKFGHRSYKTVINGIQPDSHLNILLDNHLKSISMPPSGILLTDYLGEILGVKAGDLLM
                     VEALEGSRAVRQVPVVAMAKQYLGVMGYMNLEALNRLINEGDAISGAYLVTDETHREK
                     LFQLFVQMPRVAEIVVRQNEIRSIYEIMAKAMLFFMFIATVMACSIAFGVVYNSARIS
                     LAERSRELSSLRVLGYTRGEIAYILLGELGFITLVAIPLGFIIGYWICAYVAHALTTD
                     LYRIPMMIELKTYAQAALVVIVSAVISGCIVRHKLNNLDLVEVLKTRE"
     gene            13742..14938
                     /locus_tag="PLF77_00510"
     CDS             13742..14938
                     /locus_tag="PLF77_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPC07363.1"
                     /translation="MNHVLRRKIFILSIMAVVAGLLIYGFLPKTRDVDIVFVTRGPLQ
                     VTIEEEGRTRLKDRFTISAPTAGYLQRIHAKVGDNVQQGQIVAVLEPLQSQALDPRSH
                     AAARAAVSAAEAALKAAVEREKVAAADAAYLDQRRERLKALYDKGSIARDQFDQIHAE
                     AQKARALQLSSAAEVHRAGSELEQARVALRNFSAVRSQGNAVAVSSPVNGAVFRIYHE
                     SEGAVNIGAPLMDLGDPKNLEVRVEVLSSDAVKIRQGTHVLFKRWGNDEPLTGRVRLV
                     EPAGFTKISSLGVEEQRVLVIVDITSPPEQWSVLGDGFRMEAHFTIWEDDNVLQIPAS
                     ALFRAGKDWAVFVEHDGKARKRVIEIGQRNGLTAQIISGLKENEKVIAYPDDTISEGT
                     KIRQRK"
     gene            14965..15501
                     /locus_tag="PLF77_00515"
     CDS             14965..15501
                     /locus_tag="PLF77_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07364.1"
                     /translation="MKVFIIVGMPAAGKNIARIYAGSRDIVYYSTGDIVRAEVKARGL
                     EVDAATTSEVSDDLRGEDGLGVTRLALEEILKTGADVGFLEGMRSWQEIELIREKTDG
                     VVVAVLTPRGLRLERVCSRGRDDDSPLGFNERDQREIAYGTAIPIALADAYILNTGTM
                     DDAMEQLDAIVRREMAPA"
     gene            complement(15937..16677)
                     /locus_tag="PLF77_00520"
     CDS             complement(15937..16677)
                     /locus_tag="PLF77_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC07365.1"
                     /translation="MKITFLGTNGWYDTDCGNTICTLIETKTAYILLDAGNGIYKADK
                     YIKKNLPVYLLLSHFHIDHMEGLHILAKFRFTSLEIIGQTGTKKTIGEFLSPKYSLPV
                     TRLPYPCNVTDINEGWHRRPFRFRALPLEHASRCFGYRLEMEGKVISYCTDTGYCKNS
                     EELSVNADLLISECAFLSGQQSKTWPHMNPQLAARLAVKSQAKRLALTHFDAALYTTR
                     AKRAKAQMQAKRIFPHTFAASDGLSIVI"
     gene            16887..>18919
                     /locus_tag="PLF77_00525"
     CDS             16887..>18919
                     /locus_tag="PLF77_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPC07366.1"
                     /translation="MTNDIITLNEFDGTALLKKGGIPVMDSILARDDKEIDVAANQLG
                     FPVALKICSEKILHKSDRGGVALNIADTSSLADAFRDMNNRFSDVPHAFLVQKMARPG
                     TELILGARRDPVFGPVILAGIGGIFTEIFRDTAIDLAPVDNTIACAMLGRLRGAALLD
                     GYRSQKALDVVAIAEALSSLSRLISDRPDILEIDVNPFIAYPDGAVAVDALVRLGGSH
                     ARSARKHPEPATVVPFFNPASIAIIGASRSPGKGGNIILRNLQKAGFGGAIYPINPTM
                     KEILGLPSYAKVCDVPAPVDLAMIVIPKTAVPEALEDCKAKGVTNIILSTGGYSDAGK
                     AGAVEQKIIIEQARKADIRIMGPNSIGTLNPSAGMATSIVGLEPIKAGGVSIIGQSGV
                     FSSGWARWIADTKPFGLAKVACIGNKGDINESDLLEYLAEDWVTLTVGMYLEGVIDGR
                     RFIRAAEKASRKKPVVVMKAGRSEAGAAAVASHTGSLAGSDAVFDAVCRRTGLVRTHN
                     PEAFFDTLSAFEKLPLPRGNRMGVFSITGMGCVAATDAAEEYGIVLPELNPATLKKLA
                     EVIPAWAPVRNPIDTWSAIEQHGSKKTSIHIAQCMLDQRDMDALLIIFVLMPESMFDI
                     PEAFAELFRNHPDKPVFASYYGGTAREIAHVHEGFSRLGVPCYPTPERAMFAFG"
     assembly_gap    18920..19019
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            19546..21051
                     /locus_tag="PLF77_00530"
     CDS             19546..21051
                     /locus_tag="PLF77_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07367.1"
                     /translation="MKQVAWSKSRRPKSKPIFYKNLLIMMAIAVLGIAMVPVTGQAVS
                     RVPLNASGIYSATADDGSKIFLYRYAPYTTGTPKFNTSATPVLIFTGITMNMNQYLSC
                     TPPDKKDVYSSVYVPPVDQAPEWALNEDKTDYEPYIKADKMRYYSLAHYLWLKGYDPW
                     FVNYRGTGRGEVRSDGTNANSLTTLDTWATQDAPAAIAKVKSVTGKRMFIGGHSTGGL
                     VSYDYLQGAYMESGIASSAWAKKAYYKLCYSLGYMPHVRASADLAKKRNEDVKGFIGI
                     DPAGVPSLPNLLDTPLFWTIVGSRLYLPLDYLSDNLIQVLPSKPLVGLLEGMFGLLNK
                     AAAGDSAISDFFDYLNFWVVEDMDPCLEDWTVRYAVGSAAIRGFGHYMDMGLNNTLRE
                     HYLNGKENYLSSKLVKGVSAPNPGRDGYYYYSENMARMTVPMIVFSSSTGSLVSPQAT
                     YDFIISKKTPTAYDEWYVLDGTGHFDLAMGKKMPTEMFPRLGAWLDTVDAL"
     gene            21301..21870
                     /locus_tag="PLF77_00535"
     CDS             21301..21870
                     /locus_tag="PLF77_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPC07368.1"
                     /translation="MEQTFTITVNVQELFMEFIPKLAGQYIQMRGDQEELKGTELKIT
                     LDISGSVYSYIIQDGVGFSIREGAVDNPHVRIAFSPDTLSKMADMKNIDMLIAMQKQL
                     TRKKFDVLTALSGKGVFQIKNADDTVSEITAIFNGAEKPIAVLRLSMQDANMISGGKE
                     SPINLFMSGKMKIEGDIAFAMKLQPLFVA"
     gene            21937..22380
                     /locus_tag="PLF77_00540"
     CDS             21937..22380
                     /locus_tag="PLF77_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07369.1"
                     /translation="MLMPIKRIFFKTRLAFLMYGFACMMRLAVLISKDFRAHLATHDY
                     SFIMGSPEEDTYRYFQFIRGRVQSVRGRRPSDFALIWRDNRSGGKVMIDLAAGKRKAL
                     NRAVVHGLLTLEGEGKYISLLIETMNRLNRLFRHERKTNNRVKAG"
     gene            22790..24610
                     /locus_tag="PLF77_00545"
     CDS             22790..24610
                     /locus_tag="PLF77_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPC07370.1"
                     /translation="MSEFKPYNDTTIPGIFRNQVEKSRDIIFLRIRDENKKWKEYTYG
                     DISGQVDALASYFLKKGIQPTDKIAIYANNRPEWAVADLATLAIGAADVTVYPTNSGP
                     EAEYILNDSRAKICMCAGKFQVDNLLKEKNNLHYLQEIIVFDEIDSRYPIVVKFSSVL
                     QIGKASLNLDEIDKRLRNIDVQETATLIYTSGTTGNPKGAMLTHSNLVSNVKQFVEHH
                     PFPPRGTCVSLLPLSHALERTVGYNSAMHEGCTISYTKGAETLLGDLKDVRPTLAVYV
                     PRVLEKIYEGVMSQLGNAPPSKQKLFHASMGVGKKALPYILYNKPVPFFLNLQYKLFE
                     KLIYSKLRKALGLDRMVTIGVGGAPLSNTINEFFQIIGVEIHLGYGLTETTPITHLNT
                     YKHLKPIKIGTCGPAFPRTECKIAEDGEILIRGPQVMKGYYNRPEDTAQVLTKDGWFY
                     TGDIGTIDEDGYLTITDRKKDILITAGGKNVSPQVIEGAFVMHPLIEQIAIMGDQKKY
                     LVALIVPCFTELIKWAKSKNINETDPTKLIAYPEVVQKYKETIDELNKPLGRVEQIKR
                     FSLINHAFSQESGELTPTLKIKRKIVLANYQDRIEEMYKE"
     gene            24724..24921
                     /locus_tag="PLF77_00550"
     CDS             24724..24921
                     /locus_tag="PLF77_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07371.1"
                     /translation="MHYRICKTVTIVLFTMLAMFCLSCTEAKCKIDQTVCNYDCPSTI
                     GVKQACEQKCNLLYDICRSQK"
     gene            25186..26700
                     /locus_tag="PLF77_00555"
     CDS             25186..26700
                     /locus_tag="PLF77_00555"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HPC07372.1"
                     /translation="MTTPIQQKIRDKKRTAKQIAEMVKSGDWINHGAVGGDSTACTEA
                     VAKRLGGGKSDLHDIEFWLAGNFYPHPELQEIDPMQQYHCVHEHFFFPWNRKAREDND
                     VTSWTPWGWAGGIWAHHYRFANGERKNRGIDWWFNAATAPDNHGYFNMSYGTNSSNIF
                     KQTAKKIVFEVRADYPWAEGGRFNTIHIDDIDYWVEVDCEKYKWPQMNEKGIVARPVE
                     QEIASHIMTIMRDRDVVQLGIGSLPSACVAAMADAGFKYLGIHTEMLNYGLIKMIESG
                     QVTNAYKRLDQGKSVWTFAFPVDVKWYYETVHRNQALAVYDIDYTNNLDVLRRVDNMI
                     AINNTVAVNLLGQMTCSFYGKRPISGTGGFFQFIIFAAQSKGGRSVAAMTSCSKHNTP
                     RIVPFLPEGSVVDVPAQFAQYVCTEYGMVNLRGLNGYERARALISIAHPDDRAWLEKE
                     ARRHGLMPPQFPANFLPEEGGNRRYPSYSDRRSYKMPMGSSIWGYDWDPIQSGK"
     gene            26780..27622
                     /locus_tag="PLF77_00560"
     CDS             26780..27622
                     /locus_tag="PLF77_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07373.1"
                     /translation="MKVIPADASTVVLLRSCPDASVRGIEVLMVLRNRKSSFVPGYYV
                     FPGGGLENDDFADGMERFIVGMDRLAASKILSDMRYPEQALGAWVAGIRETFEEVGIL
                     LAQKKDGSPVTIRAENERQRFCDHRKALISGKMRFIEMLATEELLLPLDRLYYFSHWI
                     TPEPFPQRYDVRFFVTEIPSDQAVIHDGVELTDHVWVRPAEAIRQYEQGRLDMVLPQI
                     MTLENIKNCKTVAEVIARVRKRNVPAILTKIKRIDGKDVEVMPDGAVFENRPPAYSWP
                     DKSD"
     gene            complement(27687..28199)
                     /locus_tag="PLF77_00565"
     CDS             complement(27687..28199)
                     /locus_tag="PLF77_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07374.1"
                     /translation="MMQKTKWFMARFIFLTAAMGTALSGGLLGYVLCPLFSWYFFKDL
                     NFIKYHHYIIRLVFAFWRQVVELLYNPDYREMFYIPWTDPPINAPDPKRVRVRALWQH
                     SDKGCGLCNNCCTRRACPLHDMKHNQCKSYGSFFWRYFNCGRYPENTKQIHYYECKKW
                     ERYNCLSENE"
     gene            complement(28202..29671)
                     /locus_tag="PLF77_00570"
     CDS             complement(28202..29671)
                     /locus_tag="PLF77_00570"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HPC07375.1"
                     /translation="MLLNIYGLSVEAAIIIFLFIWLLIFAGYYYYSGVAAEEFTANEN
                     DATQPHSRWPVLVLFLVMIFAIVYSNSYKSWQMSEPLHIAWLNGHGCLRQAPQGIFNK
                     RPLSGEKQSTTSAEPYEGITPRPLVLITVDALRSDQMGVYGAPRETTPFLSHLWRGGK
                     LRRIDNAYSICTTSFCGMIGILSSKYWHQLNEPPVNLADVLKKYNYRNSFLLSGDTTN
                     FFGLRQVLGSHIDLFCDGSIEPKKYANDDRSVLGWLQTYNWKSADRTFLYIHLMSAHI
                     IGLRDPRFKKWLPDKEWDIPKLFYSQRAYQNNYHNGVLQADNTIRQIFEILDREGMLQ
                     RALIVITADHGEFLGEFDNFGHGFEPFEPMVRIPMLVYDGLQSNYPERSLSSQVDVAP
                     TMLYAIGAAIPKDWSGIPFQLAAPRKAVYTACYDVSGVVADIGGQRFKFLQKRKGGKE
                     MLFDLDTQKAESINLAAKPDMRKILASMRELNQLTQKSK"
     gene            complement(30165..30959)
                     /gene="trpA"
                     /locus_tag="PLF77_00575"
     CDS             complement(30165..30959)
                     /gene="trpA"
                     /locus_tag="PLF77_00575"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HPC07376.1"
                     /translation="MKPIENKFSELRARNEKALIVYLTAGDPSLDITKQLIFALEKSG
                     VDILEIGVPFSDPTADGPVIQAAAQRSLKAGTTLKGVLKMVADVRKTSDIPIVLFGYF
                     NPIFAYGVKNFAQAARHAGVNGVLVVDLPYEEAKELRDYTDAVGMDFISLIAPTTDKE
                     RLKKIASKASGFLYYISITGITGTAAPKIGNIKTEVSKIRKITKLPIAVGFGISRPEQ
                     AREIGRFADGVVIGSAVIRLIDENKNNGDLSKILSDYISNIKKELR"
     gene            complement(30959..32152)
                     /gene="trpB"
                     /locus_tag="PLF77_00580"
     CDS             complement(30959..32152)
                     /gene="trpB"
                     /locus_tag="PLF77_00580"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HPC07377.1"
                     /translation="MKNTLPDKNGHFGIFGGRYAAETLMPALIELEKAYKIAQKDRGF
                     EKEFEWYQREYAGRVTPLYCARRMSESLRGAKIYLKREDLNHTGSHKINNTLGQALLA
                     RRMGKKKVIAETGAGQHGVATATVAALFGMECKIFMGEEDIRRQAPNVFRMKTLGAEV
                     VPVKSGTATLKDAMNEAMRYWVSSVRDTFYVIGTTAGPHPYPGMVRDFQSVIGKETKR
                     QIMKFEGRYPDVMIACVGGGSNAMGTFYPFRNEKNIAMYGVEAAGHGMNTSEHSASIN
                     GGKAGVLHGNKTYLLQDAHGQVRDAYSIAAGLDYPGVGPEHAYFHATKKVKYVTITDK
                     EAIKAFSFLSLKEGIIPALESAHAIAYAMKLAPKMTKNKIIVICLSGRGDKDAQTIIE
                     KGVMG"
     gene            complement(32149..32811)
                     /locus_tag="PLF77_00585"
     CDS             complement(32149..32811)
                     /locus_tag="PLF77_00585"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF002298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HPC07378.1"
                     /translation="MTQVKICGIASKGDAFAAVNCGADALGFIFYPPSPRYIKPDDAE
                     KIIRVLPDKVVKVGVFVNEQPKNIKKLVETCGLDMIQLHGDESPDDCRLFPSQVLIKA
                     VELKNEFDLTPACAYPVAAILVDNRHAGRYGGTGKKSNWDLAAGLRGKIPLILSGGLN
                     AENVADALKTVTPDALDINSGVEASPGKKDHQKLARIFDTIRAFDTKHENFQSIFKKR
                     DT"
     gene            complement(32813..33583)
                     /gene="trpC"
                     /locus_tag="PLF77_00590"
     CDS             complement(32813..33583)
                     /gene="trpC"
                     /locus_tag="PLF77_00590"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HPC07379.1"
                     /translation="MLDKIIETKKEEVAQLKKETTEAQLKKEIAGFAPCRDFKKALTS
                     SDCNIIAEVKCASPSRGRLVTDFDPLRIARIYEQNGAAAISMLTDEKYFSGHKDYLTQ
                     IRQIVQLPILRKDFIVDPLQIYETRAIGADAVLLIVRVLGEKLADFISLSGEVGLSQL
                     VEVHSQEELDLALAAGAEIIGINNRDLDTFITDIETSVKLKKRIPENNLVVAESGIRK
                     RKDIELLIKAGIKTFLIGEHLVTAPDIGKKLRELKGES"
     gene            complement(33797..34807)
                     /gene="trpD"
                     /locus_tag="PLF77_00595"
     CDS             complement(33797..34807)
                     /gene="trpD"
                     /locus_tag="PLF77_00595"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HPC07380.1"
                     /translation="MIKEAIEKATMKTDLPEAEMMAAMDEIMEGVATPAQIASLITAL
                     RMKGETVEEVSGAARIMRQKATRINACSSTIIDTCGTGGDKLNTFNISTTAAFVAAAA
                     GLTVAKHGNRAVSSGCGSADVLEALGVNISADQEIVEECVQQIGIGFLFAPKLHGAMK
                     YAIGPRREIGIRTIFNMLGPLTNPAGATAQLLGVYDAKLTEMFAHVLKNMGTKKAFVV
                     HGLDGLDEATITGETRVAELRDGIVRTYNINPVDYFGRLDSLESIRGGDPSANAQITR
                     DVLSGKNGPCRHVVLLNAALAIVAGEKAKDIKEGLAIATDCIDGGAAVKKLQGLIEMS
                     NS"
     gene            complement(34823..35392)
                     /locus_tag="PLF77_00600"
     CDS             complement(34823..35392)
                     /locus_tag="PLF77_00600"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HPC07381.1"
                     /translation="MILMIDNYDSFTFNIVQYLEQMGEKVNTYRNDKIKTDDIRRLQP
                     RAIFLSPGPCSPAEAGVTVEVIRKFYQDVPIMGICLGHQSIGYAFGATVSRAGRIMHG
                     KISHVKHDGQTIFAGLPNPFPAGRYHSLIVYPETLPDCLEISALTDEGEIMGLRHKKY
                     PVEGIQFHPESVLTPQGKRIIRNFLIYTK"
     gene            complement(35482..36978)
                     /gene="trpE"
                     /locus_tag="PLF77_00605"
     CDS             complement(35482..36978)
                     /gene="trpE"
                     /locus_tag="PLF77_00605"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HPC07382.1"
                     /translation="MYQPNFAEFEKLAQKGNLIPVYREILADVETPVSVLMKLRKKDH
                     VFLLESVEGGEKWGCYSFLGTDASVVFRVQGDRVIIDEKGNVTTRHHHGGPLNFLREL
                     LNRYKPVALPGLPRFYGGAVGYLGYEMVRYFEELPPSPPDDLNLDDAVFIISDSLMIF
                     DNVRHTIKVVACTFTEDEESLEAGYAASCRKIEEMIEIISASASHKIAAATEGDVNFQ
                     SNMTCHQYKDAIEKAKEYIAAGDIIQVVLSQRFETEYLNSSIDLYRALRFINPSPYLF
                     FLKMDDLTLIGSSPEVMVRKEDDTMELRPIAGTRPRGRTEQEDRALSDELLSDEKERA
                     EHVMLVDLGRNDLGRVAQTGSVQVNQFMMVEKYSHVMHLVSNVRAQLTKGKDAFDVLS
                     ATFPAGTLTGAPKVRAMQIISELEKVKRGPYGGAVGYFSFSGNMDFCITIRTMILKNG
                     RIYVQAGAGIVYDSIPESEHQESLNKARGMLQAIKLAAGGFEISTNQA"
     gene            complement(37354..37545)
                     /locus_tag="PLF77_00610"
     CDS             complement(37354..37545)
                     /locus_tag="PLF77_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07383.1"
                     /translation="MKKIRRSLKPAGGSTTRVKPQRENVWCERYGYFIDLDACRARSM
                     QNKACRRCFPSLIQVPLPF"
     gene            37674..39134
                     /locus_tag="PLF77_00615"
     CDS             37674..39134
                     /locus_tag="PLF77_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HPC07384.1"
                     /translation="MMLLESFLSAVKSIRVQDVLDVMIIAIMIFALLTWFKTRASRFV
                     LIGIILLGGVYIAARFFQLYLTTIVLQSFFAILLFVLVVIFQEDLRRFFERLAMLGSL
                     GKKFPEISGLKSTAEIIAQTAANLAKKKTGALIVLRGNDPLERHINGGTKLDGVVSGA
                     LLESIFDPHSVGHDGAVIIEDNRISQFGCHLPLSANTSKYSNLGLRHTAALGLSERSD
                     AICVVVSEERGTISLAYMENLTVVPSAAVLHETLEQFYIRNTPTKKSHPALNWLKENT
                     LEKVIAIAMACILWMAFGYQRDIVRRDFIVPIEYKNIPRNWQIDEMQVTDARVVLKGP
                     EQAFRLLDERSLKLSLDLSGIADKKREFNLSADMINVPSNLTIADIVPAKIHVTASKR
                     IPWTFPVHVNTQNALAHHLSLQRLSVAPSKVRVLISAQTDPDQIKIETEPVDLKRILS
                     TTTVDTKIVLPIGVALPEGQSPAARVQIKVKQKASS"
     gene            39146..40207
                     /gene="corA"
                     /locus_tag="PLF77_00620"
     CDS             39146..40207
                     /gene="corA"
                     /locus_tag="PLF77_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPC07385.1"
                     /translation="MTSKYHKRSRKTGLAPGSLVHIGSPYAEKSKITLMQYNEGFFEA
                     KDLESLKDFQADKDKEIITWLNVDGLTDIQLFEEIGRIFGLHPLVLEDILNTAQRPKM
                     EDYGGYIYLVARNFHDQENGSLLFEQISIILGKNFILSFREKESNLFKSIREKISQNK
                     GRIRKENTDYLAHALLDNIVDNYFVILENLEEKIEALEDDLIRQPDSSSLQAIHMLKR
                     KLILLGKSLWPLREAISALERSDSALINKTTVVYFKDIYDHTIAIIDTVETFKDMLSG
                     ILDVYLDTVSNRLNEVMKILTVIATIFMPLTFIAGIYGMNFKYMPELDMRWGYFGILG
                     VMLLVALFMVRYFRKKNWF"
     gene            40279..40758
                     /locus_tag="PLF77_00625"
     CDS             40279..40758
                     /locus_tag="PLF77_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPC07386.1"
                     /translation="MSVFAASDIVEVAIRIEENGVNFYKFAEQIAKKEDEKKLFAGLA
                     QAEVAHKKTFEKLFAGMEKSNLQESYEGEFGEYLRAYVDNNLIFTKEIMDKQLAKVTD
                     TTSALEFAIQRELDSILYYHEIKKMVPAAQHDALEKIIEEERKHFQALTAMKKRLAC"
     gene            complement(41034..41804)
                     /locus_tag="PLF77_00630"
     CDS             complement(41034..41804)
                     /locus_tag="PLF77_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07387.1"
                     /translation="MSNTNDQNLSQIIIKLKPKLIFLYIFVPIFFLVMAHILGQLAIF
                     NIIPCFRGYDLFSVFFNLDGEANIPTLYTILEWFLCVIFLVLIAFFERNTQSSRYRYW
                     LGLAIIFSFLLIDEFISFHEISDHILALFNIKIVLFPTNAWVLIYLVVLAPISLVYLR
                     FMIHLPKKTKYLFVFSFIIFMLGAAGIEALELRHVEIYGQDKYYYLYFVIVEETLEMM
                     GLLIFIYALASYLANELGISQIKILPEKISHESTDKSA"
     gene            complement(41813..43177)
                     /locus_tag="PLF77_00635"
     CDS             complement(41813..43177)
                     /locus_tag="PLF77_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC07388.1"
                     /translation="MQKLSILIVDDEKSQRELLRDFLRSEGHTVTEADNGEKAIKMVL
                     GGHFDLILLDYKMPGMNGLEVLKEVKRINPEIDVVIITAYGTVETAVDALKAGAIDYI
                     TKPIVELDELIILVNRIAERRQLIQENRLLKDDLSKHGITADKIIYQSRQMAELINLA
                     GRVAPSKASVLIQGESGTGKELLARLIHQLSPRADKPIIIVNCVALHENLLESELFGH
                     EKGAFTGASSRRIGRFEEADGGTIFLDEIGELSPTIQVKLLRFLQEREFQRLGSNVNL
                     QVDVRFISATNRDLEIQVAEGTFRDDLFYRLKVVTMSLPPLRERKEDLPVLIDYFIDK
                     FAKENGKNIKGFTAEARDLLLKYDYPGNVRELENILERAVVIAREQYITTNDLPFKSD
                     FLLEDSEKKPFGALKDSLDELEKKLIIEAMEKTQNNQTRAADLLGISERMLRYKLKKY
                     GLKG"
     gene            complement(43199..44986)
                     /locus_tag="PLF77_00640"
     CDS             complement(43199..44986)
                     /locus_tag="PLF77_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC07389.1"
                     /translation="MRLFRKKDIVVHRKAHNYNDPFLQPLAVAIVSAIFVALILITGF
                     LELRRSESNLIAFMENQALSTIGVLQRLTEENLKSIIGVPDKNAPHEKTSGRDEASYS
                     KQWVIQAITELAKDLDEQWRKGDMTDESLRQFASDNNFSYVGMLNAQGNAVFQSGPLQ
                     RDLLEENELTHKGRKLTTLELISKIGEKQGVGFIALSRKDNTGAVIISLDKSGSVYWI
                     LKIAGERGMDKIGEGHGIVYMQIFNDRDKLLSSVGQPAPALNHRIFNLKEILSGKIKI
                     VSRKVDYADNKILDLAAPLYLDKKIVGIVRIGLDRNSMDKSLVENRQHIFVFSIFIVL
                     IALLSMWLLYHDQNRHLAGIVEMERQLEKAERLSSLGQLAAGVAHEIRNPLNAISMAT
                     QRLKRDFVPSDPQKVDDFQNLSGIIRDEIKRLNGIIEEFLNFSKSRRLDFCKFSITEL
                     LQKIVSLMKEEALLRGITLETTWKQESVPISMDVNKLQQAFINLIKNAMESITAEKGR
                     IVISVDKEGKNYIVVKISDSGCGMTEEELEKIFNPEYTTKEKGVGLGIPLAFEIIRGH
                     GGDIRVTSRKNKGTTFEVILPRERLNEVS"
     gene            complement(45729..47078)
                     /locus_tag="PLF77_00645"
     CDS             complement(45729..47078)
                     /locus_tag="PLF77_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HPC07390.1"
                     /translation="MKTLDLFDQGNSGNVKDAAPLAERMRPQNIYEYIGQKHLLGEGS
                     LLKRAIESDKLFSMIFWGPPGSGKTTLARILANETKSKFVSFSAVLSGVKEIRAVIDE
                     ARELLERKSIKTILFVDEIHRFNKAQQDAFLPHVESGLITLIGATTENPSFEVIAPLL
                     SRTRVLVLKPFSEENLLAILEAALKNKQKGLGNIDVKIDANVLRYLVSLADGDARSAL
                     NNLEVLVSMVQNLPDDQRAISLKFAQDALLKKSLLYDKDGEEHYNLISALHKSLRGSD
                     PDAALYWLGRMLAAGEDPLYIARRMIRFASEDIGNADPQALVVAVAAQQAFHFIGLPE
                     GELALAQAAVYLATAPKSNALYVGYGKVKNTINTKGYLPVPLHIRNAPTKLMKDLNYG
                     KDYKYAHDYEESYVPQDYLPEEIKGELFYQPKGVGFEKTIKERLNYWRNKKKEGEKE"
     gene            complement(47075..47278)
                     /locus_tag="PLF77_00650"
     CDS             complement(47075..47278)
                     /locus_tag="PLF77_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07391.1"
                     /translation="MTFAYCLREGGNLPCVRIIRCWSPVFDIESFLKGHLSEKRWLKF
                     INTKAPDKITSLIELIEAAKAKK"
     gene            complement(47414..48778)
                     /locus_tag="PLF77_00655"
     CDS             complement(47414..48778)
                     /locus_tag="PLF77_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HPC07392.1"
                     /translation="MEQVKVVLQDREIPKKWYNIMADLPTPMKPPLHPGTGQPVTPQD
                     LAAIFPMNIIEHEASNKRWIKIPEEVLQKYLLWRPSPLYRAYNFEKLLNCPVKIYYKN
                     EGVSPAGSHKPNAAVPMAYYNKVAGTKRITTETGAGQWGSALAMATQMFGLECRVYMV
                     KISYEQKPYRRLMMNTWGAECIPSPSTLTQSGRDFLAKYPDSPGSLGIAISEAIEDAV
                     TSKIKTHYALGSVLNHVLIYQSIIGLEAEKQMQKLGIYPDVVMGCCGGGSNFAGIAAP
                     FVRDKIHGKDVKIVGAEPSSCPTMTKGPFAYDFGDLAQKTPLLPMYTLGHDYVPAPIH
                     AGGLRYHGMAPIISHLVKEKLIEPRAYDQLKTFDAGVKWARSEGFIPAPETNHVLAAV
                     VDEAMRAKKEGKEKTILFNWSGHGIIDLAAYDAYFAGKLKAYELPGHEIERALKAIKN
                     FPKP"
     gene            complement(49051..49998)
                     /locus_tag="PLF77_00660"
     CDS             complement(49051..49998)
                     /locus_tag="PLF77_00660"
                     /inference="COORDINATES: protein motif:HMM:NF016356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 and DUF1722 domain-containing protein"
                     /protein_id="HPC07393.1"
                     /translation="MEKIRLGISSCLLGNNVRYDGGHKLDRFLTDTLGKYVDYVPVCP
                     EVECGLSIPRPSMRLEGHPDAPRLMVTSTRQDLTERMETWAKKRVTELEKEDLCGFIF
                     KSDSPSSGMERVKIYNEKTMPVKNGVGLFAAMFMKHFPLLPVEDEGRLHDPGLRENFI
                     ERIFALKRWRETLSAKNFKKSLIDFHTKHKLLILAHSTKHYQMMGKLVAAQKNFAGPD
                     IQKEYQALLLESLKVKATPAKHVNVLQHMMGYFKDQLSGDEKKELLELIHAYRDGYIP
                     LVVPVTLISHYVRKYDEPYLKEQIYLNPHPSELQLRNHV"
     gene            complement(50192..51607)
                     /locus_tag="PLF77_00665"
     CDS             complement(50192..51607)
                     /locus_tag="PLF77_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPC07394.1"
                     /translation="MDLIQKYLMKDIDVLLNCIDEAILIVDRDANILKYNETFSRLTD
                     LMGRSLVGMNLKDIVASGILRESAALKSLEVKKKIDMNLTYETGQTATWTYIPVFDER
                     GELVMTVGTGRDMTKLVTLEKKLKISETIISQYEGRNLKMAGDAGQVEIIYSSSVMQQ
                     VIRLGWKAAATSSPVLIWGETGVGKEIMAALIHQLSARSAKPFVAINCASIPDELLEA
                     ELFGYEEGSFTGAKRSGKKGLLEEAHEGTLLLDEIGELPLKMQSKLLRFLQEGNFQKI
                     GSNKTQAVDVRILCATNLTMDQLMNSKQFRQDLFYRIAVIPVFIPPLRDRQEDIPPLI
                     LHFIQIFNTKYKTDIKLPAKLMNRLYTYEWPGNVRELKNVIERMAIMHASQELTEEDL
                     ELILNINPRQASSDRSLPAPEPAMPVLPPSLPKAMDQFEEKWIRQAYQMTGSIVGAAK
                     LLGINPSTIHRKLKKGILRLD"
     gene            51786..53495
                     /locus_tag="PLF77_00670"
     CDS             51786..53495
                     /locus_tag="PLF77_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPC07395.1"
                     /translation="MSNQKPWLKFYKNVPETIDYPRVTMYEALMQTVERYPDRIAYDF
                     FDYTSTYRQFAQDIDICANALASLGLKKGDRMTISMPTSPQGIICFYAANKLGVVASM
                     IHPLSTPAEIAYYLNVSKSRVALTLDLFHNSFKNLPEKTPLELLILTEIFDQLTQAQP
                     MAEKPLPEEGPAVRWWKELMTAAHPQAPKADMHTDEMAVILYSGGTTGFPKGIMLSNF
                     NFISEGLMVANWGGGELMAEDSSVLAILPIFHGFGLGVCCNAAFMGGSKSILVPQFTP
                     ENVADLIRKKRPSIIVGVPTLYDALSKNEVFQTTDLSCLKNTSSGADSLPRTVKDRFE
                     EVVKRQNGNVQLVEGYGLTEAVTAIMSTPIDGYREGSVGIPFPDMLAKIVGIGTIDEV
                     PVGQEGELCLHGPAVMLGYLEQPEETASVLKTHADGKVWLHTGDIFTMDADGYFYYKL
                     RLKRMIKSSGMNVYPVQVEACLDQHPDVAAVCVIGVPDEAQVEKVKAFVVLKDATKAS
                     AEMEKELINFSRKHLIKWSCPREVEFTDKLPLTKVGKIAYTELAKQEIEKLRKAGKYT
                     GGK"
     gene            53495..54616
                     /locus_tag="PLF77_00675"
     CDS             53495..54616
                     /locus_tag="PLF77_00675"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPC07396.1"
                     /translation="MNHLEEYRRAVSDPSDYVQQLKKASGKKIIGYTCSYTPEEVIMA
                     AGAHPLRLFGTKQHISLADSHLQSYCCSVVRGILEEGLSRRVDYLDGMVFPHTCDSMQ
                     RLSDIWRMNIPFGFHLDITLPVKLDTASAKDYMIDVLKKFRDDLGGKLNVEISDDAVQ
                     KAIATTNAIRQSVRAIYDIRNLHPRLMPGEDLYNIVRASMIMDRDRLAVMLAETAAEL
                     KERATSAKPASVKRILMAGGMCNQPDVYAMIEESGGEIVWDDFCTGARYFTGLTDAGS
                     DPLSSIGERLLNRVVCPAKHADLDGRARHIIQLAQEKNAQGVIFLLPKFCDPHAFDYP
                     YLKERLEKAGIPLMNVEVDDSLPAEGQLKTRFESFLEML"
     gene            54628..55866
                     /locus_tag="PLF77_00680"
     CDS             54628..55866
                     /locus_tag="PLF77_00680"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HPC07397.1"
                     /translation="MSDTQDTEKKTVSYTKQMRDIMTAYYIDAKTARENNKKVAWITS
                     GGPVEPLIAMDIIPVYPENYGAMIGASKMGVDLCKKAEDMGYSSDLCSYARGDISCAT
                     VKGGPIGGLPEPDMLVCCNNICGTVLKWYEVQARYFKVPLFIFDTPIVHTEFTREMKT
                     YVQRQMEEYIVFLENVSGRKMDRDRMVEVGKLSVEGQQLWQEVLDTTMNRPSPMTAFD
                     AFFYLALIVTLRGTKQAVDFYRGLRDEMRIRASEGKGAIPRERYRLLWDNLPIWYRLK
                     WLSQEFAKHDACLVADTYTSAWCGSMKYIDRNDFIGSAAETYTRIYLNIGVDKMAEIV
                     LSMIDKYQADGIVMHSNRSCKPYSFGQYDIQKIVERERGIPSLIIEADMCDERSFSEA
                     QIQTRIDAFMEMIRAKKGAS"
     gene            55985..57328
                     /locus_tag="PLF77_00685"
     CDS             55985..57328
                     /locus_tag="PLF77_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HPC07398.1"
                     /translation="MKVRKIILAVICTLMLAATAVMASGPSLQGEELYKYLSKKTISE
                     GDAAFVEGLNTSSLKLDWSAAQKKDLTGNLRILKVKNAKVGTEALYFIRGSEGDMYVV
                     SLPAEGGRPAAEYEKISENRMMFALETVTGEHEGTTYAFARISGEPKAILFDRIFKIA
                     IVLMLFFVMVGMGLTLKLSDFALVFRKPLGIVIGIVLQYGLMPLVAFGLSYMFGFYQA
                     YPFIFMGLILATASPAGVTSNLLTQFAKGDLALSISLTSISTILAMFCTPLLLSLYGN
                     TSSGASVPALLIAQTIIVLVIVPLAVGMTVRAKFEKAALKAAPIFSILGIITVLFIMV
                     TGVVGNLNALKDTARYSLSFYLAPLILALTGMVIAIVVAKIFRVSPKQVRSLAFETGV
                     RNSALSMTLAILIQDQIGDFYSSLFIVNGVYGLEMYIAGILFVMFFRKFSKETEG"
     gene            57436..57522
                     /locus_tag="PLF77_00690"
                     /pseudo
     CDS             57436..57522
                     /locus_tag="PLF77_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611470.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            57682..58047
                     /locus_tag="PLF77_00695"
     CDS             57682..58047
                     /locus_tag="PLF77_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07399.1"
                     /translation="MKDVTFLTHKGVRILYEDLENAKQEDVLPWIERAKGVIRSQPEK
                     SVLALLNVKNAKFDTEVTAALKEFAKGNEPYVKFAAVYGIEGLKEIIFKSVLTFTGRK
                     NLVLCKTLEEAKDILASKV"
     gene            58296..59360
                     /locus_tag="PLF77_00700"
     CDS             58296..59360
                     /locus_tag="PLF77_00700"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPC07400.1"
                     /translation="MKIIKAQKLAYFDTSHEMPLEIIMAAGFTPYKILGDVHQSNDPA
                     DKHLSNFFCGAAKSWLTEALARSKEWAGIIFAQGCNNTNRHYDIWKVHVQTPFLHWFG
                     APLLDSDAAKKYYRVELQRLISHLENQFNIKITPEKIREAIIKSNEIKKKLQRLSALR
                     AQKDIPNRDYLAAMIKCLTNPVDEVSAWLDSEIAAWNSKPSFPSGKKQFLLTGSDITY
                     VEWMDTLEDCGIRIVRDDLSVGERFFATLIPDKSDPLDALIEYYMNIPKPATRPTIQK
                     RLDYLYKALGETKVDGVLSQNVKFCEPFAYDSVPVNNALKDKGYKVIHLERDFSPAAD
                     QQLANRLMAFVEMMEVGNNG"
     gene            59353..60633
                     /locus_tag="PLF77_00705"
     CDS             59353..60633
                     /locus_tag="PLF77_00705"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPC07401.1"
                     /translation="MAKTNNSAENDRKIKLKTLPHAKDLKWPMLWWFTMGSIYHALPW
                     KKTAWTCAAFPIELMWIYDIFPDHPENMATVAAARKQSQRLIEHAEGMGYSRDLCSYC
                     KTSIAAYDTKIKKTLGGISKPDIVVCTNAICDTHWKWFQIMADKMNVPFFMFDIPKWV
                     SGTDEKTLQGNIDYVVEQFYDLIVFIEKHTGKKMDEKRLYKVLDKSTELSDLWREIFE
                     HRKAVPSPYSGAETSASFFPLVVLPGVQVGINFFKKILADIKNLTAKGEGTMPKDKEK
                     YRVMWEGIPFWYRMRFMYDLAQYGAVITFEPYTYSFAPEKKKCNTMEETLRDVAKLIM
                     DTPYAYNLERRIEHFEKWIDEYKIDGVILHENMSCRPSSAGMVDLAEAIMRDKGIPVL
                     ILQCDMNDPRAYSEGQIKTRVEGFIELMEANKGK"
     gene            60820..>61372
                     /locus_tag="PLF77_00710"
     CDS             60820..>61372
                     /locus_tag="PLF77_00710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPC07402.1"
                     /translation="MITAGIDIGSITTKAAIFADGKITATKVIFTGYNAEAAGLKVYE
                     EALKEAGIDKNAVAKIVSTGYGRNSVKFADKSYTEIMCHATGAHFLNPHIRSIIDIGG
                     QDSKAILLDDKGKVKNFVMNDKCAAGTGRFLEVMARAMEVNLDEFGAMSINSNQPAKI
                     SSLCTVFAESEVISLIAKGEQRKD"
BASE COUNT        15575 a        14691 c        15277 g        15629 t
ORIGIN      
        1 ccagtttggg aaccggcata tgcaaatctt cctgaagggg atcttccgcc gcccggccgg
       61 cagtagatat ttcccgcgga tccggcatgc attgggcgta aatgggatcg tgaaaatcct
      121 ggtgttggat aagcgataaa tagtaaggcg ttacggccat cggataaaca gccgttaccg
      181 actggagggt ttcccggtgg ctcgcggcgg tttgcggctg cgcgcacggg aggagcggat
      241 gggtgaggcg gtttttgatc tgccagcgcc agtcggccca gtcttttacc gaggtttttt
      301 gaatgaaagg tgaaacagtt tttgtcttca taaggatctt tttttaccaa acaatttgag
      361 gctggctttg tatcacaaaa ttaggtgctt gcaaagccgg cgaattgcga ttagaagaaa
      421 aacacagcgg gcctgcaata cagatggcca attttaatta cgaggcaata ccgtgaaaga
      481 tatgcagcag cgcaaagaaa acgtggcaat gttgtcggtt gcttccaatg cgtttctcgt
      541 tttattcaaa gtccttgtcg gctttgccat cggttccgtt tcgatcattt cggaagcgat
      601 tcactccggt gtggatctgc tggctgcagt gatcgccatg ttttccgtca aaacttccag
      661 cgttccggcc gacgaacgtc atccgttcgg ccacggcaaa attgaaaacc tttccggctt
      721 tgtggaagcc ctgctgattt ttggcgcggc gttttggatt atctttgaag cgctgaaaaa
      781 actaaatgcc ccccaggcca tggaaaatgt cggctggggg gtcggcgtca tgttgttttc
      841 tgccgtcatg aattttattg tttcgcaaaa actttttaaa gtgggcaagg aagccgattc
      901 cattgctctc caggctgatg cctggcattt gcgcacggat gtgtatacgt ctgccggtgt
      961 catgtttggc ctgacgatca tctggctggg tcataaattc ttcgccgacc ccaatattta
     1021 ctgggttgat ccggttgccg cgatcttggt cgccgcgctg attatgcgcg tcgcatatcg
     1081 tttgaccgtt gacgccctca gtggcctggt tgatgtccag ttaccgaggg aagaagagaa
     1141 ttttatccgc agcatgatca gcgtggaaaa ggccgtctac ggttttcatc aattgcgcac
     1201 gcgcaaagcg gggcatgttc gctttgtgga atttcacatc aaagtcgatc cgcagatgtc
     1261 cgtccacgat tcgcacggca ttaccagaat tttgaagaaa aacatcaggg ctcggtttcc
     1321 ggaatccacg atcaccattc atatcgaacc ctgtgatggt gaatgttccg atatgtgtca
     1381 ggccggttgt ttattgcccc gggagaaaag accggataaa ggcatgatcc gaccgcaacc
     1441 gatcggacat gatcaatcat cgtgaagagc tgctgtttat aatagacaaa tcggcggggt
     1501 gacgggcggc agtttatcgc cttcgtacag gaccttttcc agaaaaagac cggaaggcgg
     1561 ggcggtgaag tgcgcgggca gctctgaaaa ggatgtcatc attttttcga catcgggcct
     1621 ggtgaggttg ccgcggccga gctcaacggt cacgccgaca atgcgccgga ccattttcca
     1681 gagaaaatgc gaccctaaaa tgcgcaccgc aatgatattg tcgaattctt tcagccatag
     1741 gccttcaatg ttgacttttg tggaagcttc cttgtccatt cttttgtcgg cgaaggaggc
     1801 aaaatcatga aagccctgaa aaacatccag cgtctgtttc attttatcgc aatcgagtct
     1861 gtctttgacc caccagacat atcgcttgcc gaaagccgtg cgtcttcggg aaatcaaata
     1921 gagatagctt cggctgagtg cgtgatggcg ggcatgaaaa cgcgcgggcg cattttccac
     1981 ccttaagatg ttgacgcttg acggcaggtg atcgttcata cccgtgcgta aaatgtccgg
     2041 atggactttt ttgggagatt cgagatgggc cgcttgctcc agggcatgta ccccggcgtc
     2101 ggttctgccg gccccctgta ttcttaccgg cacgtccaga aaactttgcg ccgccttgat
     2161 taaagtttcc tgaacgctgc gggcgttttt ctgcgtttgc cagccgcggt aaccggttcc
     2221 gtcatattca agcagaattt taaatttgta cgatgccatt ttcagtgaag gtgttgttta
     2281 atacgtcgtc ccttatgacc tattttctta aacagcggcg tcaacgggaa gacatgctat
     2341 ttactttgcc ggcgccagta tcgtcatggc ggaaagattt ttgatcagca aggcatcggg
     2401 atcgatgttt gttaattctt tgccgggatg caaggtcgtg atgacggcct tgccgatgat
     2461 cttgcgtcct tcctcgccgc gggcgtctgt gattctcagt ccccagacat tgtgaaaaat
     2521 cagtgcccgc ccgtttcgtt cgccgatgta aagcatgata tgtcccctgc gccacagcag
     2581 gctcaaatag ggaatgccct gttccagaat cactttttct ttttcttgcg gttccatagc
     2641 ctccaggtca atataaatgc cgattttttt tgcctgatcc gccgaatgtc tgggcagcca
     2701 gatgccgaaa ggagcaaaaa aatcccgcgt cattgcggaa caatcgcggt ttccgtaaag
     2761 accgccccag ccgtagggtt cgtcaatcag ttcattggca attctggttg cgtttgcata
     2821 ggtaaacggc aacggtttga tgttcatgat ctcccgcgaa atgaaaacgc gttggataat
     2881 ggcctgatgg tttgcgtccg ccgaggcgat gagaatttcg agtttatccg ccatctcggt
     2941 gatcagagga aacatttgtc cgatagaggt ccggagactg aaattgccgt ttgcgtcgac
     3001 gatcgggatt ttatcccggg tgacggtggc atagcgggag gtttgccatg tctgcataaa
     3061 gggatcgtca acgcgtgcaa aatcctcagc cggaatccat ccgaaggcaa agctggtttc
     3121 caccagggcc catgaccggt ccctgctgat gtggcatatc agaatcggtg tgttggccgc
     3181 cactgaagat atctgaagat tgtcaaacgg ccagccgttg atgtcgccgc ccggtttata
     3241 aaaatgtgga ccgttcgtgg gcagcatgcg caggttcgtc gatcggacgg tgatcgccgg
     3301 ctgcagcgta ttgggataat tttccagcca ggcgttctgc tgaagttttt tcaaccatgc
     3361 ggtggaatgc tttctcttgt tttcgccata gcccggatgg gcggcaaatt gtgtgaaaat
     3421 ggcagacaat ctttccggca gtgcgtgcac cggttcagga tggtgccaca cggaaaaaaa
     3481 cgttttgtcg taatgttcat tcatcgcagt ttgactatca cgggtcagaa ggactttgtc
     3541 tgccggcacg tccgccagac gggcggtgtg atcctggggc aggtcggtga catcttttac
     3601 gtcctggagg gtatcgggag gttgcaaata acaggacact aaaaacagcg ctaaaacaac
     3661 gcagacgtat tttactttca tgcccgatcc cttcaattta ttctggcgta ttgtatagtc
     3721 ttttttcatg ttttgggcaa gatgaaacgg cgaaaaattt tctgtatttt ataaataatt
     3781 tatggaataa gggatcgaac cctgctatac aaacttttgg attttcgacg ggcagggcga
     3841 aaagttttaa aaatacggcg ccgtttacgg gtagaagaag gagatgaacc atggatagtt
     3901 ttgtgcccaa aaagattttt tttacaaaag gagtgggcac gcacaaagac gaattgcatt
     3961 cctttgagcg tgccttgcgt gatgccggaa tagagaaatg caacctggtg caggtgtcca
     4021 gcatctttcc gccacgctgt aaaatgatct ccaaaactca ggggctgaaa caattgacgc
     4081 cgggcgccat cacgtactgc gttatgagcc gttgctgtac gaacgaaccc aaacgactca
     4141 tggccgcttc ggtcggttgc gccattcccg ccgataaatc atcctacggt tacatcagcg
     4201 aacaccatgc tttcggtcag aatgaaaaac aggccggcga ttacgcggaa gatcttgcag
     4261 cggccatgct ggcctccaca ttgggcatcg attttaatgt tgatgagagc tgggacgaaa
     4321 agaaggaaat ttttaaaatc agcggcaaga tcgttcgaac gctaaatgtg acccagtcga
     4381 caatttgcaa agacaacaaa tacaccaccg tgattgcagc ggcggtattt gtgttttaaa
     4441 tatttttaaa agcgtataaa cggcgtatga atccccgaag ggagacatac accggtgaca
     4501 tgcgaccttt tccccggtgc ggactatgac ccaaacggaa agaattctgg atattccgtc
     4561 tctgaaccac cacgatatcg gcggagcgca tttaccgtat ctttattacg agggagagac
     4621 tccgcaaatt ctttttgccc acgccaccgg ttttctaccg tggctttggc atcccatcct
     4681 ggaggagctg acgccgccca ggaggcaggc gtgggcgcca tatatatgta attaccgcga
     4741 ttgcgatccc gaaagcggcg ggctttcctg ggatgtgatt gccagagacc tgatcagctt
     4801 ttgcagcgtt cagaatatca gcgataatct ggctgtcggg cattcgatgg gcgccacggt
     4861 ggtgacgatt gcctccgcgc tttttggtct gcagccccgc gggatgattc tgattgaacc
     4921 cattttcctt ccggaagatt tttataccat cgatatccag ctcaaagatc atcccctggc
     4981 ctctttgtcc atccgaagaa aaaacggctg gaaggatgaa aatgccgcgc tggagtatct
     5041 gaaatcaaaa tcgctttttg cgggctggga cgaacagatg ttgcaatttt atctgcaata
     5101 cggcatggaa aaacaggccg gcggcgattt gaagcttacc tgtgcgcccc gcaacgaagc
     5161 ggctcttttt atgggcggta aaagcaccaa tccctggccg ttgctggaaa agttgacctg
     5221 tcccgtcctg attctggaag gggaaaaaag ctccaacaga gagtttatcg atctgcccaa
     5281 ggcagcatcg cttctccggg acggccggta ccagttgatt cccgatgccg gtcatctgat
     5341 tcccatggaa aaacctaaag aaatcgccaa aattataaaa gaatttctgg cggacgtctt
     5401 ttaggaaatc attgacgaaa tgctttaaat atgtaaaatt tttacgctag cattccggga
     5461 acaatgaaag ggaagaaatc tgttgatagc tttggatgat tacaagcaga ataggactgg
     5521 agacacacag aggttcaggg gaacggtgag caaaccattt gccttgatcg ggcggaatgt
     5581 catccggtgg attaacagca tcggagcggc cactattttt ctggtgctgg ctttactcaa
     5641 aacctttcat cccaagcagt ttcaaaaggt cattcagcaa atttattata tcggggcgcg
     5701 atcgacgacg attatcatgc tggtcagtct gtttaccggc atggtgctcg gactacaatc
     5761 ctatcatgcg ctggtgcagt tcggcgcgga aggatcgctg ggaacgctgg tctcgctgtc
     5821 gctgattcgg gaactggggc ctgtgctgac cgcgattatg attacggcca gggccggatc
     5881 ggcaataaca gccgaaattg gcgtgcagcg gatttccgag cagattgacg ctttggaaac
     5941 catgcgcatc gatccttttc gctatctggt gactccccgg atcaccgcgt cgatcgtcag
     6001 ttttcccctg ctaaccactg tattcgatct gatcggcatt atcggcggtt atgtcagcgc
     6061 ggtggctctg atgggattga atgccggcgt ttattcacac agcattcggt cctacgtgga
     6121 tatggaagac ctgaccaacg ggtttgtcaa atcgcttgtg ttcgcggtga ttgtcgcgac
     6181 cgtgtgctgt tatcagggat attttgttca tatgcgcgag gacagcaaag gcgccaaggc
     6241 ggtcggtctg gccaccacct cagcggtggt tatttcctgt gtattgattc tggttgcgga
     6301 ttacgtcgta acgtcactcc tgatggtata gaaaagatat gaacacaccg ttgattgaat
     6361 tcaaaaatat caccaaacga ttcggagaac aaaccgttct cgatcaggtc aatttgcaga
     6421 tttacgaagg ggaagtaacc actattatcg gcctgagcgg ttcaggcaag agcgtgctgc
     6481 tcaagcacat cattggcttg ctcaaacccg atgaaggaac cattcttttc cggggagaac
     6541 cgatcaacga catgaagaaa acagaactgg ccgaagccat gtccaaaatc agctacatgt
     6601 ttcaaagcaa tgccttgttc ggctccatga cggtttatga aaacattgcg ctgccgcttc
     6661 tggaaaatac ggccatgaaa aaaaacgaaa ttcatgaccg ggtcatggcc agaatcgaac
     6721 agacggaatt gacagaagcc gcacagaaat atccttcgga gctttccgga ggcatgcaaa
     6781 aaagagcggc gctggcacgg gcgttgatta ccgatcccca ggtggttttg ttcgatgaac
     6841 cgacaaccgg tcaggaccct gtccggaaaa acgcgatttt gagcatgatc gcccagtatc
     6901 agaaaaaatt taattttacg gcggtcatgg tcagtcatga aattcccgat gtgtacttta
     6961 tttccaaccg gattctcgcc ctctatgaca aaagcatcgt ctttcaggga acaccggagg
     7021 aattgagtaa ttttgatcat cctttcaacg atgaagtgat ccgcagtctg gaaggtttgc
     7081 aaaaggaatt aaccggtctt tattccagaa gacaatttaa ggttttgcac cgttcccagt
     7141 tacaaagccg caagtccgac gaaggctatt gtgtggttgt gtttgattta tcggatatga
     7201 attcgattgt ttccgccatc ggttacgaca gggcgcagga aaccatccat tcgatgggaa
     7261 agtacatcga taaacatttt gatgccatcg gcggtttctc cacccgtcgt aaaatcaatg
     7321 aattcgtgac agtgcttcct ttttccgata ttgccgagac ggaaagcatt gtcaaagact
     7381 ttatggagga tttccaaaaa agtggcatga gtgagatatg gagcgaagct caaaaaaacg
     7441 atcctcagac gaggtgcgtt gatttttcag tcaaagccgg tatggcggaa gggacaccgg
     7501 tcgctgaaat tgattcaatt gtaaaactgg ccaaagctca tcgaaaagaa atcggacggc
     7561 tgcaatgtgc cgtcaaggag taagcacatg aaaaaatacg agattgaaac tgtcgtgggn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna tgtgccgtca aggagtaagc
     7741 acatgaaaaa atacgagatt gaaactgtcg tgggaatttt tgtgtttctc ggtcttctct
     7801 gtatcggata tatgaccgtg aaactcggaa atgtgtcttt tttggaagcc gcgtcctatc
     7861 cccttgaagc taaatttacg tccgtcaacg gtttacggat cgacagttcc gtaaaggtca
     7921 tgggtctgac tgtgggcaaa gtgaaacaaa ttgccatgga tcaggacaat caacgggctg
     7981 tggtgaaatt aaatattcaa aaaggcatca agatttatga cgatgccatc gcctcgatca
     8041 aaacggaagg attgatggga gacaaatatg tcagcatcga tccgggcggc ggaggctctc
     8101 tgctggagcc cggtggaatt attctggaaa cgcagtcacc gttggacata gagacgctga
     8161 tcagcaaata cgcttttggt gatgttaaaa aacaatagat cggttattac gacgttgagg
     8221 gaggaaaaat tcatgaaaaa actttttacc caaacagcca tcatcctggt gattgtaatg
     8281 ttttctctgc ctgtttatgc gggcgcggcg ctggattccg ttcagaaatg cgtcaacgag
     8341 gtcatcggta tcgtgggcga tcccaagctc aagagcgagt cggccaagga actgaaaaaa
     8401 gaaaaactcc gggttctata caaacagatg ttcgatgaaa ttgaattttc caaacgcacc
     8461 ctgacgcgca actggaataa ttttacgcct gcccagcgga cggaatttgt caatcttttt
     8521 gagcaggtgc tggagacagc gtacggcgat aaagtgctca gctataccaa tgaaaaggtc
     8581 gtcttttata aagaaaccac gctttcggca acccaggcgg aagtgcaaag caagatcgtc
     8641 acctcttcca aggaaattcc tgttttttac cgcgttattt taaaagacgg aaaatggaaa
     8701 gtatatgatg tcgtggtgga aaacgtcagc ctggttcaga attatcggac gcagttcaac
     8761 gatattttgt ccaaggacaa accggaaaaa ttgctggaag ttctcagaaa caaggttaag
     8821 cagcattaaa aaacctgctg accctgcggc tgtgttgcag cacgccggct gataaccttt
     8881 catacggtcg gatgacatga aatctcgttt ggtatttctc gtatcgttta tatggatgct
     8941 gtcggggggc gcacccggca gcgctcatgc atttgttgat ctgccgccgg agcagacatc
     9001 tccgccgcct gtggtcctga cagtctcccc ggaccggccc gcaaatcaat tcgccgcggc
     9061 cgctgtggtt gatcccatgg aagaggaata cacgggggac gaattcgatg actatgcggg
     9121 ggacaatgct aaagaagaaa aagtgtccat cgccgatcct ctggaacctt tcaacagggc
     9181 aatgcatgag ttcaacgata aaatgtattt ctgggcggtc aagcccgtag ctcagggcta
     9241 taaagccgtt gtccccgaac ccgcgcgcat tagtgtggat aatttctttt ccaatctcgg
     9301 atttcccgca cgatttttaa gctgtctgct gcaggctgat ttcaaaggtg cagcgacgga
     9361 agcgggacgt tttaccatta ataccgtctg gggcatcggc ggtctgctgg atccgtcttc
     9421 cggcaaggag ttgaatttac aggagcagaa cacggatctg ggacaaactc tcggcgtctg
     9481 gggcgtggga cagggttttt atattgtctg gccggtcttc ggtccctcca ccccccggga
     9541 taccgtcagc atagccggca attattttct ggatcctgtt gcctacctga atccctggtc
     9601 ggcccgcctg ggtgtgaatg tcctggataa ggtcaacgcc gcatccctga gaatcggcga
     9661 ttatgaagcg ctgaaaaatg cggccatcga tccgtatgtg gcttttcgcg acgcctacat
     9721 tcagtaccgt tacaagcaaa ttaaagccag aaaggcaaaa attttctccg gatcatcggc
     9781 agcagaaccg tttccggaga cggtccggcc ggacaaggtg gattaaatga aaaaaatacg
     9841 ttgcgcacca tattggatgg tttttgttct gacggcgtta gtttttttta caaccggtcc
     9901 ggcggcaagc gccgcaggat tgttcagcgc ctttgagacc gtttcccgtc aggacggcgg
     9961 attgaatacc gccatcggtt accggtatca ccaggataca tatgaaaaag gcaacgggtt
    10021 gaagatcagg cagaatgaga tttattcgca ggcggcctac ggtgctgaca atgcctggga
    10081 aatatatgcc cggctgggga ttgccgatct gaaaataggc gacgttttca gagaaagcga
    10141 agaggtcttt gcaactctgg gcggaaaggt tttttacccg gtcagcggga agctgggagt
    10201 cggcgcattt gtccagggga cctattattt gaatgatttt tccgataccg ttgagcgcag
    10261 gaagcatgtt caactgaagg atctctggga tgttcatttc ggtgtcgcct tgcaggcgac
    10321 gctgccctgc ggctcgacga tgtatgcggg gccgtattta tattactcgg aaggaacgct
    10381 gtctctttcc tccaatgtgt cggactgggg atatgctgcc ggtcgttatt ctgttcagaa
    10441 cacgtccatt gccggaggtt ttgcgggtct ggatatcccg cttgccaaag gttttcgtct
    10501 gaacgttgaa ggacaatatg cggacaggtt ctccgccggc gccgccatca gctatacata
    10561 ttaagattat tcctgcacat tataagtcaa cccgcagccc gtcagacaac cggtgagtcg
    10621 gatgaatcag cagaaagaaa aatttttcca ggtacaggat cttaccaaag tttatcaaat
    10681 gggcgaggtg gccgttcatg ccttgcgcgg ggtgaccatg gaactctatg ccggcgaact
    10741 ggttgttctt ctcggtccgt ccggaagcgg caaatcaacc ctgctgaaca tcatgggcgg
    10801 cctcgataca gccacgggcg gtgtggtgtc ctacagagga aaagaactga ccaaagccac
    10861 ggaaaaggaa ctgaccgagt accgccgttt tcatgtcgga tttgtttttc aattctacaa
    10921 tctgatttcc agcctgacgg cccgggaaaa tgtttcagtt gttacggaga ttgctcccga
    10981 tcccatggcg ccccagcaag cgcttgacct ggtcggcttg tctgacaggt tgaaccattt
    11041 tcctgcgcag ctttccggcg gcgagcagca gcgcgtggcc atcgcccggg ccatttccaa
    11101 aaaaccggct gtgctgttgt gcgacgaacc gactggcgcg ctcgattccc aaacaggcat
    11161 tgtggtgctc caggccctgg agcgtatcaa ccgcgaactg ggcacggcta ccgccatcat
    11221 tacccataac gtggatatcg cgggcatggc cgaccgcgtc attcatttaa gcaacggcca
    11281 tattgccagt gttgaaatca acaaagtgaa gaaatccgcc agcgagctgc agtggtaaga
    11341 tgatgaaaac gctgaaccga aaactctggc gggaattgtg gctgatgaaa gggcaggtgt
    11401 ttgccattac gctggtggtg atgagcggcg tagccacctt catcatgttt atcagcacca
    11461 tgcattctct cgattttacc cgcagcaagt tttaccgcga atacaatttt gccgatgttt
    11521 tcgtcaattt aaagcgggcg ccggaaaccc tcaaatataa gatcaatgac attcaaggcg
    11581 tccatcaggt ggaaaccagg gtttgcgccc aggccaagct ggatatccgg ggattcaagg
    11641 aatcggtgac tgccaggata acctccattc ccgatgacgg tgatcctctg ctgaatcgtc
    11701 tttacatccg taaaggacgt cttcccgatc ccgcacggga taatgaagtg gccatgaacg
    11761 aaaccttcgc gcaggcgcat ggcctgaatc cgggcgataa gtttgcggcg gtcattaacg
    11821 ggaaatggaa gagactgacc gtcaccggca ttgttctttc tccggaattc attttgctga
    11881 tgaagacggg cgccatgacg ccggatttca aacggtacgg cgcattatgg atgacccgca
    11941 aggctatcag caaggcctac gatatggaag gcgcgttcaa cgatgttgtt ttaaccgttt
    12001 atccgggaac ggcattgagt gatgttgtgc gggatctgga caacattctg ggacggtacg
    12061 gcggctttgg cgcttacgga cgcaaggatc agatttccca ccggctgttg agtgaggaat
    12121 tccggcagct caagaccagt tccagaattt accccacgat attcatctgt gtcgccgcct
    12181 ttctgctgaa cattgtcatg agcagaacca tcagcacgca gcgcgagcag atcggcacgc
    12241 tcaaagcatt cggctataat aatctcagca ttgccgttca ttacgcgacg atggtgatga
    12301 tgattattgc cacaggcgtg ttgctgggga ttgccggcgg tatctggttc ggcaaaatgc
    12361 tgggggcgct gtacatggaa acctaccggt tcccgttttt tcattatatc ctccatccgc
    12421 aagtgatgat agcggtcgtc gtgatcagtg ttgccgctgc gctcagcggg acgatgcatt
    12481 cgttgtggaa tgccgcccgg cagactccgg cagaagcgat gcgaccggaa gcgccggtga
    12541 aatacagggt atcgctgatt gagaaaactg gcatcggccg ctggctgtcg cagccgtcgc
    12601 gtattatctt ccgcaacatt gaacgaaagc cggtcaagac cgtgctggcg atcatcggca
    12661 tcgcaaccgc ctgcgcgacg atgatctgca gcggtttttt ccgggactgt attgattaca
    12721 tggtgcacat tcaattcgtc ctgtcgcaga aggaagatat gaccgtaagc tttgtcgaac
    12781 cgacgtccta ccgggcggta tatgaactga aacgcatgga aggtatcagc agcggcgaag
    12841 cattccggag tgttccggtg cggatgaaat tcggtcatcg cagttataaa accgtcatca
    12901 acggcatcca gccggacagc cacctgaaca ttttgctgga caaccatctg aagagtattt
    12961 ccatgccgcc gtccggtatt ctgttgacgg attatctggg agagattctc ggagtgaaag
    13021 cgggagatct gctgatggtc gaggcgctgg aagggtcccg ggccgtccgc caggttccgg
    13081 ttgttgctat ggctaaacag tatctgggag tgatggggta catgaacctg gaagcgctca
    13141 accgcctgat caacgaaggc gatgccatct ccggcgccta tctggtaacg gatgagacgc
    13201 acagagaaaa acttttccag ttgtttgtgc aaatgccacg ggtagccgaa atcgtggtcc
    13261 ggcaaaacga gattcgcagc atttatgaaa tcatggccaa ggcgatgctc ttctttatgt
    13321 ttattgcaac agtgatggcc tgttccattg ctttcggtgt ggtgtacaac agcgccagaa
    13381 tttccctggc ggaacgcagc cgcgagctgt cttctctgcg cgtgctggga tatacccggg
    13441 gcgagattgc ctatattctt ctgggtgaac tggggtttat aactcttgtt gccattccct
    13501 tgggatttat tataggatac tggatatgcg cttatgtggc tcacgctctg acaaccgatc
    13561 tataccgcat accgatgatg atcgagctta aaacgtatgc gcaggcggcg ttggtggtta
    13621 tcgtatcggc ggtgatatcc ggttgtatcg tccgtcacaa gctcaacaac ctcgatcttg
    13681 tcgaggtgct caaaacccgg gaataaaata aaaaaactca gatgggtcag aggatcattt
    13741 catgaatcac gtgttacgca gaaaaatatt cattctcagt atcatggcgg tcgttgccgg
    13801 cctgctcatt tacggttttc ttcccaagac gcgggatgtg gatattgttt ttgtcacgcg
    13861 agggccgctt caggttacca ttgaagaaga aggccgcacc cgcctgaagg accgctttac
    13921 catttctgcg cccactgccg gatacctgca gcgcattcat gccaaagtgg gcgacaatgt
    13981 gcaacagggc cagattgttg cagtactcga acccctgcag tcgcaggcgc tcgatccccg
    14041 cagccacgcc gcagcgcggg cggcggtttc cgccgccgag gcggctttga aggcagccgt
    14101 ggaacgcgaa aaagtggccg cggcggatgc cgcgtatctg gatcagcgca gggaaagatt
    14161 aaaagccttg tatgacaagg gctccattgc cagagatcag tttgaccaga ttcatgccga
    14221 agcgcaaaaa gcgcgcgccc tgcaattatc gtctgcagcg gaggtccata gggcaggttc
    14281 cgaactggaa caagccagag tggccttgcg gaatttttca gccgtaagaa gccagggcaa
    14341 tgccgttgcc gtatcttcgc cggtgaacgg agccgtgttt agaatttatc atgaaagcga
    14401 aggtgcggtg aacatcggcg cgccgttgat ggatctcggc gacccgaaaa atcttgaagt
    14461 ccgggtggaa gtcctttcgt cggatgcggt gaaaatcaga caaggaacac acgtgctgtt
    14521 taaacgctgg ggcaatgatg agccgctgac gggcagggtg cgtctggtgg aaccggcggg
    14581 atttacgaaa atatcgagcc ttggcgtcga ggaacagcgg gttctggtaa ttgtcgatat
    14641 tacgtcgccg ccggaacagt ggagcgttct cggtgacggt ttccggatgg aagcgcactt
    14701 tacaatctgg gaagatgaca acgttctgca gattccggca agcgcccttt tccgtgcggg
    14761 aaaagactgg gcggttttcg ttgagcacga tggaaaagca cgcaaacgcg ttatcgagat
    14821 cggtcagaga aacggactga cggctcagat catttccggt ttaaaagaaa acgaaaaagt
    14881 gatagcatac cctgacgata cgatcagcga aggcacaaag atacgacaga gaaaataaac
    14941 taaaacactt ttgcgagggg agatatgaaa gtttttatca ttgtcggcat gccggcagcg
    15001 ggtaagaata tcgcccggat ttatgcagga tcacgggaca tcgtatatta ctcgaccggc
    15061 gatattgtcc gggccgaggt caaagcacgc ggattggaag tcgatgccgc aacaacatcc
    15121 gaggtgtccg atgatctgcg gggtgaagat ggcctgggcg tcacccggct tgccctggaa
    15181 gaaattctga agaccggcgc cgatgttggt tttctggaag gcatgcgttc ctggcaggaa
    15241 atagaactca ttcgcgaaaa aacggatggt gtggtcgttg cggtcctgac tcccagagga
    15301 ctgcggctgg agcgcgtctg ttcgcgagga cgagatgatg attctcctct gggctttaac
    15361 gagcgggacc agcgtgaaat tgcttatgga acggctattc ccattgccct cgccgatgca
    15421 tatatcctaa ataccggcac gatggatgat gcaatggaac aattggatgc cattgttcgc
    15481 cgggagatgg ctcccgcata aaaccatcaa agattaattc gtcccggttc atcggcgtga
    15541 agaagcgtac agaaaaagaa ccgtatttaa acgttatcat gtatcgaagt cgctgaatga
    15601 accgttatgc ccttcccggg atttcccaaa aaagactgac agtttcattg attcccgttc
    15661 tccgacgccg agagatgtct gcggctattc gatcgtccgg ggtccgattt tgcgatgaac
    15721 aaagcactgc ttaaaaagtg cgggtcaaga tttccgaaat acaacacatt acgtcttgcc
    15781 ccgaacctgg cgaacgagca gacaattaac aaggtgatga agcttaaaga agcaaggcaa
    15841 atcttcacaa acaggatgcc gcttacagaa gatattccaa agactttttt aaattattcg
    15901 attatcgagg acaaatccgg aatcatcatt atgctgttaa atcactatgc ttaatccgtc
    15961 agacgcagca aacgtgtgcg gaaatatcct ttttgcctgc atctgcgctt ttgctctttt
    16021 ggcccttgtt gtatataagg cggcatcgaa atgcgtcagg gcgagcctct tcgcttgtga
    16081 tttaacggcc agcctggcgg cgagttgcgg gttcatgtgg ggccaggtct tggactgttg
    16141 accgctgaga aatgcgcatt ccgagatcag caaatcggcg tttaccgaaa gttcctcgga
    16201 gtttttacag tatccggtgt cggtgcagta actgatgact ttgccctcca tctccagccg
    16261 gtacccgaaa catctggagg cgtgctcaag cggcagcgcc cggaatcgaa aaggacgcct
    16321 gtgccagcct tcattgatat cagttacgtt acagggataa ggcaaccggg tgaccggcag
    16381 agaatatttt ggagacaaaa attccccgat cgtttttttg gtgccggtct gaccgataat
    16441 ctccaaggat gtgaatctga atttcgccag gatgtgcaga ccctccatgt gatcgatgtg
    16501 gaaatggctg agcagcaagt aaaccggcag atttttcttg atgtatttgt ccgccttata
    16561 gatgccgttt ccggcgtcca gaagaatata ggccgttttt gtttcgatca gcgtgcaaat
    16621 ggtattgccg caatccgtat cataccagcc gttggttcct aaaaatgtaa tcttcatctt
    16681 gcccccgtat tgttacttat aagattacga aatacttttc aaattataaa atcgttttta
    16741 atgagaatga aaaaatcttg tagagattca ttgaagtcat tcgaaatcac cggaaaagac
    16801 gaactctggc ttgacaagat atcgcggcgt gtgttcatta tgaaaacgtt tgtttcaaaa
    16861 cagtggaatc aggaggtgtt ttagttttga caaatgacat cataacactc aacgagtttg
    16921 acgggacggc actgctcaag aagggcggaa tccctgttat ggatagtatt ttagccaggg
    16981 atgataagga aattgacgtt gcggccaatc aactgggttt ccccgtagcg ctcaagattt
    17041 gttccgagaa gatacttcac aagagcgacc ggggtggcgt tgcgctgaac attgcggata
    17101 cctcctcgct ggccgatgct ttccgggaca tgaacaatag attttccgat gttcctcatg
    17161 cattcctggt gcagaagatg gcgcggccgg ggactgaact catcctgggg gcaaggcgtg
    17221 atcccgtgtt cgggccggtg atcctcgcgg gaatcggcgg catctttacc gaaattttcc
    17281 gcgacaccgc catcgatctt gccccggtgg acaatacaat tgcgtgcgcg atgctcgggc
    17341 gcctcagagg agcagcgctg ctcgacggct accgctctca gaaggctctg gatgttgttg
    17401 ccatcgccga agccctctca tccctctccc gattaatatc cgatcgaccg gacattctgg
    17461 aaatagacgt aaatcctttc atcgcgtatc cggatggtgc cgtggctgta gatgctctcg
    17521 tgcggctggg tggcagccat gccagatccg cccgcaaaca tcccgagcct gctacggtcg
    17581 ttcccttttt caatccggcc tccatagcga ttatcggtgc ctctcgaagt ccgggcaagg
    17641 gcggcaacat tattctgcgc aacctgcaaa aggccggttt cgggggcgcc atttatccga
    17701 tcaatcccac gatgaaggaa atcctgggtc tgccctctta cgcaaaagtc tgcgatgtgc
    17761 cggcgccggt ggatttggcc atgattgtga ttcccaaaac tgcggtgccg gaggctttgg
    17821 aagactgcaa ggcgaaggga gttaccaaca tcattttgag cacgggcggg tattcggacg
    17881 caggcaaagc cggcgccgtg gaacaaaaaa ttattatcga acaggcccgc aaagcggata
    17941 tccggatcat gggtcccaac agcatcggga cgcttaatcc ctccgccggc atggcgacgt
    18001 ccatcgttgg ccttgaacca atcaaggcag gcggcgtatc gatcatcggc cagtcggggg
    18061 ttttttcgtc aggctgggcg cgctggatag cagacaccaa gcccttcggg ctggccaagg
    18121 tggcttgcat cggcaataag ggagacatta acgaaagcga tcttctggaa tacctggccg
    18181 aggattgggt aaccctgacc gtcggcatgt acctggaagg cgtgattgac ggacggcgtt
    18241 tcatcagagc cgccgaaaaa gccagcagga aaaaaccggt tgtcgtgatg aaagcaggac
    18301 gcagtgaagc cggagcggcg gctgttgcgt cgcatacagg ctcgcttgcg ggatcggatg
    18361 cggtgttcga cgctgtctgc cgcagaacgg gtctcgtgcg aacacacaat cccgaagcat
    18421 ttttcgatac cttatccgcc tttgagaaac tccccctgcc tcggggaaac cgcatgggtg
    18481 tgttttccat cacgggcatg ggttgcgtcg ctgcaacgga tgccgcggag gaatacggca
    18541 ttgttctgcc cgaactgaat cccgcgacgc tcaaaaaact ggcagaagtg attcctgcat
    18601 gggcgccggt gcgcaatccc attgatacct ggtcggccat cgaacagcat ggttcgaaaa
    18661 agacatcgat ccatattgcc caatgcatgc tggatcaaag ggatatggat gccctgttga
    18721 ttatttttgt tcttatgccg gagagcatgt tcgacattcc ggaagcgttt gccgaactat
    18781 tccgcaatca tccggacaag ccggtctttg ccagttacta tgggggcacc gccagggaaa
    18841 ttgctcatgt ccatgaagga ttttccaggc tgggcgttcc ctgttatccc acaccggagc
    18901 gcgccatgtt tgcgtttggn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
    19021 caggctgggc gttccctgtt atcccacacc ggagcgcgcc atgtttgcgt ttggccgcat
    19081 ggtggaatac gcgcggcacc gcggtagaat ccgcacccgg ccttaaattg atgaatggca
    19141 attgaggacg gcttccttga tgaatccgtg tcttaaattc actcgtcgca gcggcgtcgc
    19201 ccatgacgac gccgtcggta atagatctgt ccttggactt gccggaactc acggccttgc
    19261 gtttgagcag ggttgctgat ccgcacccgg tccgcttttg ttttgttcgt aaatctatga
    19321 agaatccata tcgaaccaca atccatttct ttaaaataat tacatttttt atttgacaaa
    19381 acctcaaaaa agtctcatac agtacgaaat ccaaaaacac atattatctg attttttccg
    19441 cacgcgaaat ggtctgtaca ggtgctgtac ggcggggctt gaatcgtgaa ttaaaaaact
    19501 tattttgaca agttttttat aaatccaaaa gagggggaaa caggaatgaa gcaggtggct
    19561 tggtctaaaa gcagacgtcc aaaaagtaaa cccatttttt acaaaaacct tttgattatg
    19621 atggcgatcg ccgttttggg aatcgccatg gtgccggtga ccggccaggc cgtatccaga
    19681 gttcccctga atgccagcgg catctacagc gccacggcgg atgacggcag caagatattt
    19741 ttgtatcgct atgcgccgta cacgaccggt accccgaagt ttaacaccag cgctacgccg
    19801 gttctgatct ttaccggcat caccatgaat atgaaccagt acctttcctg tacgcctccc
    19861 gataagaagg atgtctacag cagcgtttat gttcctccgg ttgatcaggc tcctgaatgg
    19921 gcgttgaatg aggacaaaac agactatgaa ccgtacatca aagccgacaa aatgcgttat
    19981 tacagtctgg cgcactacct ctggcttaag gggtatgatc cctggtttgt caattatcga
    20041 ggcacaggcc gcggtgaggt aaggagcgac ggaaccaacg ccaacagcct cacgaccctt
    20101 gatacctggg ccacgcagga tgctcccgca gcaatcgcca aggtaaaatc ggtgacaggc
    20161 aaacgcatgt ttatcggcgg acacagcacg ggaggtcttg tttcctatga ttatctgcag
    20221 ggcgcctata tggaatccgg cattgcttcc tcggcctggg caaagaaagc ttactataaa
    20281 ttatgctatt ccctcggcta tatgccgcat gtcagagcca gcgctgatct tgccaaaaaa
    20341 aggaatgagg atgtaaaagg ctttatcggt attgaccccg caggcgtgcc gtcgctgccg
    20401 aaccttctgg acacccctct tttctggaca attgtaggat cgagattgta tctgccgctg
    20461 gattaccttt cagataattt aattcaggtg cttccgagca agccgctggt cggtttgctg
    20521 gaaggcatgt tcgggctgct gaacaaggcg gccgccggtg attctgcgat aagcgacttc
    20581 ttcgactatc tgaatttctg ggtggttgag gatatggacc cctgcctgga ggattggacg
    20641 gtgcgttatg ctgtcggcag cgctgccata cgcggcttcg gtcattacat ggacatgggc
    20701 ctgaacaata cccttcgtga acactactta aacggaaaag aaaactatct atcgtcgaaa
    20761 ttggtcaaag gggtttcggc acccaacccc ggccgagacg gttactacta ctatagcgag
    20821 aatatggcac gcatgaccgt accgatgatc gttttttcat cgagcacggg atccctggtg
    20881 agtccgcagg cgacttacga ctttatcata tcgaaaaaaa cgccaacggc gtatgacgag
    20941 tggtatgttt tggacggaac cggccatttc gatttggcga tgggcaagaa aatgccgacc
    21001 gaaatgttcc cgcggctggg agcgtggctg gacacagttg acgcattata aaaggcaact
    21061 gcggcataga tacatcggcc agcagaattt ttaaatcact tcaaaccggg aggatgcctg
    21121 ccaggcgggc atcctcccga aacaatgacg ctttttgaaa acccccgttc aatcatttgt
    21181 tggactatcc aatgcacctg cgaacattgc gcgatcggac cctcaacaga aaaaaaatcc
    21241 aaaaaaagtg ggtgcctttg ataaaaaagc acacaaatta aatctctgga ggaagggatt
    21301 atggaacaga cgttcacgat tacagttaat gttcaggagt tgttcatgga gtttatcccc
    21361 aagttggccg gacaatacat tcagatgcgg ggagaccagg aggaattgaa agggacagaa
    21421 ctgaaaataa ccctggacat atccggcagc gtctacagtt atatcattca ggatggcgtc
    21481 ggtttcagca tccgggaagg cgctgtcgac aatccgcacg ttcggatcgc cttttctccc
    21541 gataccctgt ccaaaatggc ggacatgaag aatattgata tgttgatcgc catgcagaaa
    21601 cagctcacca ggaaaaaatt tgatgtgttg accgcattaa gcgggaaggg tgtttttcag
    21661 atcaaaaatg ccgacgatac cgtttccgaa ataaccgcca tattcaacgg cgcagaaaag
    21721 ccgatcgccg ttttgcggct ctccatgcag gatgccaaca tgatcagcgg cgggaaagaa
    21781 tctcccatta atcttttcat gtccggtaaa atgaaaatcg aaggcgacat tgcttttgcc
    21841 atgaagctgc agccgttgtt tgttgcttga aaaccggggc ccggttaaaa aattttacat
    21901 tggacgtttt tcaatatggc gattcaatgc gtctgaatgc ttatgcccat aaaaagaatc
    21961 ttttttaaaa cccggcttgc tttcctgatg tacggttttg cctgcatgat gcggctggct
    22021 gttcttatca gtaaagattt tcgtgcgcat ctggcaactc acgactattc atttatcatg
    22081 ggctccccgg aagaggatac ttatcgctat tttcaattta tccgcggcag ggtgcaatcg
    22141 gtccggggtc gccggccatc ggattttgcc ctgatctggc gggataatcg atccggcggt
    22201 aaagtcatga tcgatttggc agcgggcaaa cgcaaggcgc tgaaccgcgc cgtcgtgcat
    22261 ggtttactga ccctggaagg agaaggaaag tacatttcgc tgttgattga aaccatgaat
    22321 cgtcttaacc gtttgttcag acatgagcgg aaaacgaaca atcgtgtcaa agccggttga
    22381 ttcgtaaaag caggcataaa ataaagcaaa taaacggatt tctgataatc tgaaaaagac
    22441 gttcttcggg gcgaaatata tcaccaatat attgtttata ttagcaaaaa tcaacccccc
    22501 tgttttttgt ccaaccttgc tcccgccgac atttcctgtt ttttcttgca atgtttatag
    22561 gaagtcagta ttattaatta tgatcatgcg atgatcataa gagttaaaca tgaatgaaaa
    22621 cttgtatttc aagacctgac cttatctgtt tgtattctat cagttcccct tctagctttg
    22681 tcagtactgc agttttcaaa tcaaaacgtg aaattacctg agccggctga tggtgttcgg
    22741 atcgtccgga cctgaagccg ggacagacat aaaatacagg agagatatta tgagtgaatt
    22801 taaaccttac aacgacacaa caatcccagg cattttcaga aatcaggtgg agaaatcccg
    22861 cgacataatt tttctgagaa tacgcgatga aaacaaaaag tggaaagagt acacgtacgg
    22921 cgatatcagc gggcaggtcg atgcacttgc ttcgtacttt ctgaaaaaag gcatccagcc
    22981 gacagataaa atcgccattt atgccaacaa ccggcctgaa tgggccgtag ccgatctcgc
    23041 cacgctggcc atcggagcag ccgacgtcac cgtctatccg accaactccg ggccggaagc
    23101 cgaatacatc ctgaatgatt cccgggcaaa aatatgtatg tgcgccggga aattccaggt
    23161 ggacaatctg ttgaaggaaa aaaataatct tcattatctg caggaaatca tcgttttcga
    23221 cgaaattgat tccaggtatc cgatcgtcgt gaagttttcc agtgtcttgc agatcggtaa
    23281 agccagcctc aatctcgacg aaatcgacaa acggttgaga aatatcgacg ttcaggaaac
    23341 ggcaacccta atttatacct cgggcacgac gggcaatccc aaaggcgcca tgctgaccca
    23401 cagcaatctg gtatccaacg taaaacaatt cgtcgagcat catccgttcc cccccagggg
    23461 aacgtgtgtg tcgcttcttc cgctttccca tgcgttggag cgcacagtcg gctacaattc
    23521 cgccatgcat gaagggtgca ccatctccta caccaaaggt gccgagaccc tgctgggcga
    23581 cttgaaagac gtcaggccga cgctggccgt atacgtgccg cgtgtcctgg aaaaaattta
    23641 tgaaggcgtc atgagccagc tgggaaacgc tccaccgagt aaacagaagc tgtttcacgc
    23701 atcgatgggc gtcggtaaaa aggcgctgcc ttatattctt tacaacaaac cggtgccgtt
    23761 tttcctgaat ctgcaataca agcttttcga aaaactcatt tattccaagt tgcgcaaggc
    23821 cctggggctt gatcgcatgg tgacgatcgg ggttggcggc gcgccgttat ccaacaccat
    23881 caatgagttc tttcagatca ttggcgttga aatccatctg ggttacggac tgaccgaaac
    23941 cacgccgatc acgcatctga acacatacaa gcatctcaag ccgatcaaga taggcacctg
    24001 cggtccggcc tttccgcgca cggaatgcaa gattgcagaa gacggcgaaa tcctgatccg
    24061 cggacctcag gtgatgaaag gatattacaa ccggccggaa gacacagcgc aggtgctcac
    24121 gaaagacgga tggttttaca ccggcgacat cggcaccatc gacgaagacg gttatctgac
    24181 gatcaccgat cgcaaaaaag atattttgat taccgcgggc ggcaaaaatg tgtcacctca
    24241 ggtcatcgag ggcgcatttg tcatgcatcc gctcatcgag cagatcgcca ttatgggcga
    24301 ccagaaaaaa tacctcgttg ccctgatcgt gccctgtttc accgaactga ttaaatgggc
    24361 caaatcgaaa aatatcaacg agaccgatcc gacaaaactg atcgcctatc cggaggttgt
    24421 ccagaaatac aaggagacca tcgatgaatt gaacaaaccg ctgggtcgcg tggaacagat
    24481 caaacgattt tctctcatta accacgcatt cagccaggaa agcggcgaac tgacgcctac
    24541 gctcaagatc aagcgcaaga tcgttctggc taattaccaa gaccgtatcg aggagatgta
    24601 taaagaatag cctaaaaaat tgtgtgaaca atgagggggt aattcgggac agcaattccg
    24661 aattacccct tttgcatctt ttttgttata cttcacacag acgtcatgga aggaggaatg
    24721 aatatgcatt acagaatctg taaaacggtc acaatcgtat tattcacgat gctggcaatg
    24781 ttctgtctgt cgtgcaccga agcgaaatgc aagatcgatc agacagtatg caattatgat
    24841 tgtccgtcaa ccatcggagt gaaacaggcc tgcgagcaga aatgcaatct gttatatgat
    24901 atttgcagaa gtcagaaatg atattccttg atctgaagtt caatgtttgc aagatgccac
    24961 cacgccggga ggtcagtcgt cacgtccgtt catcaataat gaagtaatca ggctgaatcg
    25021 tcttgctaaa attccgtggc tagtgtaaga ctttccgcca acttgttatt tatattataa
    25081 aaaaggctca atgaccttct tggtgttgaa tgtctgcacc ctgagccggt tgatggagac
    25141 attgcaagat taaatggcag tgtaaataaa gtaagagaga aggctatgac gacaccgata
    25201 cagcagaaga tccgagataa aaaaagaaca gccaagcaga ttgccgaaat ggtcaagtcc
    25261 ggcgattgga ttaatcacgg cgcggtcggc ggtgattcca ctgcctgcac cgaagcggtc
    25321 gccaagcgtc tgggcggagg gaaaagcgat cttcacgaca tcgagttctg gcttgccggc
    25381 aacttttatc cgcatccgga attgcaggag atcgatccga tgcagcagta ccactgcgtg
    25441 catgaacatt tctttttccc ctggaaccgc aaggcgcggg aggataacga cgtaaccagc
    25501 tggacgccgt ggggctgggc gggcggcatc tgggcgcatc attaccggtt cgccaacggc
    25561 gagcggaaaa accgcggtat cgactggtgg tttaacgcgg cgaccgcacc ggacaatcac
    25621 ggttatttca acatgtccta tgggaccaac agcagcaaca tcttcaagca gacggcaaag
    25681 aaaatcgttt ttgaagtcag agccgattac ccctgggcgg aaggtggccg tttcaacacc
    25741 attcacattg acgatatcga ttattgggtg gaggtggact gcgaaaaata caagtggccg
    25801 cagatgaatg aaaagggcat tgtcgcaaga ccggtggaac aggaaatcgc cagccacatc
    25861 atgaccatca tgcgcgaccg cgatgtcgtg caactgggca tcggatcgct tccctcggcg
    25921 tgcgtggccg ccatggcgga cgcgggtttc aaatatctgg gcatccatac ggaaatgctc
    25981 aattacggtc tgattaaaat gatcgaatcg gggcaggtga ccaacgcgta caagaggctg
    26041 gatcagggca agagcgtctg gacgttcgct tttcccgtgg atgtgaagtg gtattacgaa
    26101 accgtacacc gcaatcaagc gctggcggtt tacgatatcg attacaccaa taatctcgat
    26161 gtgctgcgtc gcgtcgacaa catgatcgcc atcaacaaca ccgttgccgt gaatttgctg
    26221 ggtcaaatga cctgttcgtt ttacggcaag cgtcccattt ccggcacggg tggatttttc
    26281 cagttcatca tttttgcagc ccagtccaaa ggcggtcgca gtgttgcggc catgacgtcg
    26341 tgcagcaaac acaacacgcc gcgcattgtt cccttcctgc cggaaggatc ggtggtggac
    26401 gtgcccgcac agtttgccca atacgtctgc acggaatacg gcatggtcaa tcttcggggc
    26461 ctcaacggct acgaacgcgc ccgcgcgctg atttcaatcg cgcatcccga cgaccgcgcg
    26521 tggctggaga aggaagcccg gcggcacggc ctgatgccac cgcagtttcc cgcgaatttc
    26581 ctgccggaag aaggcggcaa ccggcgctat ccgtcttatt cggatcggcg cagttacaaa
    26641 atgcccatgg gcagttccat ctggggttac gactgggatc ccattcaatc gggcaaataa
    26701 aaaaagagac aaaagtacag cggcccctgt gttcaatttg taacgaggaa ccggttgtcc
    26761 ttttcaaagg tgttaaaata tgaaagttat tcccgccgat gcttcgaccg tcgtgctcct
    26821 acgttcctgt ccggatgctt cggtaagagg cattgaagtt ctgatggttt tacgaaaccg
    26881 caaaagcagt tttgtgccgg ggtattatgt tttccccggc ggaggtctgg aaaatgatga
    26941 ttttgccgac ggcatggaac gtttcattgt gggcatggat cgtcttgccg cgtccaaaat
    27001 cctgtccgac atgcgttacc cggagcaggc gctcggggca tgggtggccg gtatccgcga
    27061 aacatttgaa gaggtgggca ttttgctggc gcagaaaaaa gacggttcac ccgtgacgat
    27121 tagagcggaa aatgaacggc agcgcttttg cgatcaccgc aaagcgctca tcagcggtaa
    27181 aatgcgtttt attgaaatgc tggcaaccga agagcttctt ctgcctctgg acagacttta
    27241 ttatttttcg cactggatta caccggaacc ttttccccaa cgctatgatg ttcgtttttt
    27301 tgtgacggaa attccgtccg atcaggccgt cattcatgac ggcgtcgaat tgaccgatca
    27361 tgtctgggtt cgtcccgctg aggcgataag gcaatatgag cagggccggc tggatatggt
    27421 tttgccgcag atcatgaccc tcgagaatat caaaaattgc aaaacggtgg cggaagtgat
    27481 tgcgcgcgtc cggaaacgca atgtaccggc catactgact aaaatcaaac ggatcgacgg
    27541 caaagacgtg gaagttatgc cggacggagc ggtttttgag aatcgtccgc ccgcgtattc
    27601 ctggcccgat aagagcgatt aattttccgg cattgcgcct gcgaaatatc ccggaagtga
    27661 ttcatattaa atattaataa cattttttat tcgttttcgc tcaggcagtt atacctttcc
    27721 cattttttgc actcgtaata atgaatttgt ttggtgtttt ccggataacg tccgcaattg
    27781 aaataacgcc agaaaaaaga accataactt ttgcattgat tgtgtttcat atcatgaaga
    27841 ggacacgctc ttcttgtgca gcaattgttg cacaacccgc aacctttatc actatgctgc
    27901 cacagtgcgc ggacacgaac acgtttggga tcgggagcat tgattggtgg atctgtccat
    27961 gggatgtaaa acatttcccg gtaatccgga ttatagagca gttcgacaac ctgacgccaa
    28021 aaagcaaaaa caaggcggat gatatagtga tgatatttta taaaatttag gtctttaaaa
    28081 aagtaccatg agaacagagg acacaaaaca tagcctagca gaccgcccga cagcgcagtt
    28141 cccattgccg ctgttaaaaa aataaatctt gccataaacc attttgtttt ttgcatcatc
    28201 gctattttga tttttgcgtt aattgattaa gctcccgcat cgacgcaaga atttttcgca
    28261 tgtccggttt tgcggcaaga ttgatagatt ctgccttttg tgtatccagg tcgaaaagca
    28321 tttccttgcc gcctttccgt ttttgcaaaa acttaaaacg ttgtccgccg atatcagcca
    28381 cgacgcctga aacgtcataa caggcggtgt aaaccgcctt gcgcggcgct gcaagttgaa
    28441 aaggaatgcc cgaccaatct tttggtattg cggcgccaat tgcatataac atggtagggg
    28501 caacatcaac ttggctggac agcgagcgtt caggatagtt ggattgcagt ccatcataga
    28561 ctagcatggg gatacgcacc atgggttcga aaggctcgaa accatgaccg aaattatcga
    28621 attctccaag gaactcacca tgatcggcag taatgacgat taatgcgcgt tgtaacatac
    28681 cttcccgatc cagaatctcg aaaatttgtc tgatcgtgtt gtcagcctga agaacaccat
    28741 tatgataatt gttttgatac gcgcgctgcg aataaaatag ttttggaata tcccattctt
    28801 tgtcaggcag ccattttttg aaacgcggat cgcgcaaacc gatgatgtgt gcggacatca
    28861 gatgaatata aagaaacgta cggtccgccg atttccagtt gtatgtttgc aaccagccta
    28921 aaacggatcg atcatcgttt gcatattttt tcggttcaat ggatccgtcg caaaaaagat
    28981 caatatggga acccaagacc tggcgtaaac cgaaaaaatt cgtcgtgtcg ccgcttaaaa
    29041 gaaagctgtt acgataattg tactttttga gcacatcggc cagattcaca gggggctcgt
    29101 ttaactggtg ccagtatttg gacgacaata tgcctatcat gccgcaaaac gacgtggtgc
    29161 aaattgaata tgcattatcg attcgacgaa gttttcctcc gcgccataag tgcgagagga
    29221 aaggcgtggt ttcccgggga gccccataca cacccatttg atcgctgcgc aatgcatcga
    29281 ctgtgataag cacaagcggc cggggagtta ttccttcata cggctccgct gatgttgttg
    29341 actgtttttc tccggacaaa ggacgtttgt tgaatattcc ctgcggagct tgtctcaaac
    29401 aaccatgccc gttaagccag gcgatgtgca aaggttcgct catttgccag cttttgtaac
    29461 tgtttgaata cacgattgca aatatcatca cgaggaataa gaccagaacc ggccatcggg
    29521 aatgaggttg tgttgcgtca ttttcatttg cggtaaattc ttccgccgca acaccgctgt
    29581 aatagtaata tcctgcgaat attaacagcc atataaataa gaaaataatg atcgccgctt
    29641 caacagacaa tccgtaaata ttcagcagca catcgatatg tcccgaatag gcgctcaata
    29701 attcaaacgt aaaaggaccg ctccagcttt tatagcccaa tatggtcata acgtaaaaac
    29761 aaagcaggaa ataatgaaca aagctcagaa tgagcaccag ggtaattcgc ctgacgacgg
    29821 aaggaacaat tttgaaagat tttaagtcaa acggaatcat taatacgatc aacagcaagc
    29881 acacaatgat ccaatgcatg aacaagcgca tgaaatatcc atcatatgta tagttgacat
    29941 atccgataac tgccgttgcc tgtaagaacc agaccagcgt taatttcatg acatgtttcg
    30001 gtggcagcca tttcagaaaa agaattttta gatggggatt tttcaaaagc aaccctctca
    30061 atgctttgct caacggaaaa gatcgaaatt aaattaacgt gcaaattcgt aatattttat
    30121 tacatgaaaa acaagaaaaa actgatgaga tcaatctgct ttcattatcg taattctttt
    30181 ttaatgttgc tgatgtaatc ggaaagaatc tttgacaagt cgccgttatt tttgttttcg
    30241 tcaatcagac gtataaccgc gcttccgatg acaacaccgt cggcaaaacg tccaatttct
    30301 cgcgcctgtt cgggtcggga tattccgaaa ccgacggcta tcggcaattt agttatttta
    30361 cggattttgc tcacttccgt tttgatattg ccgatctttg gcgcggctgt gcccgtgatc
    30421 ccggtgatgg aaatgtagta gagaaatcct gaagctttcg aggcaatctt ttttaatctt
    30481 tctttatccg tggtgggagc gatgagagaa ataaaatcca taccgacagc atccgtgtaa
    30541 tctcggagct cctttgcttc ttcatagggc agatcaacaa ccagcacgcc gttcacgccc
    30601 gcatgccggg cagcttgagc aaagtttttc acaccgtagg caaagatggg attgaagtaa
    30661 ccgaaaagga caatcggaat atcggaggtc ttgcgcacgt cggcaaccat tttcagcaca
    30721 cctttcaaag tcgtcccggc tttgagcgag cgttgagccg ccgcctgaat aaccggcccg
    30781 tcggccgtgg gatcggagaa aggtacgccg atttccagga tgtccactcc ggatttttcc
    30841 agagcaaaga ttagttgctt tgtaatatca agcgacggat cgcctgccgt taaataaaca
    30901 atcagtgcct tttcatttct ggcgcgtaat tccgaaaatt tattttcaat tggtttcatc
    30961 agcccataac ccccttctcg ataatcgtct gagcgtcctt gtcgccgcgc cccgacagac
    31021 aaatcacaat aattttattc ttagtcattt tgggcgccag tttcatcgcg taggcgatcg
    31081 catgcgcgct ctccagcgcg ggaataatgc cttccttcag cgacaaaaat gaaaaagcct
    31141 taatcgcttc cttatccgtt atcgttacgt atttgacttt tttcgtagca tgaaaatagg
    31201 catgctcggg acccacaccg ggataatcca ggccggcggc aatggaatag gcatcgcgca
    31261 cctgtccatg tgcgtcctga aggagatagg ttttatttcc atgcagaacg ccggctttgc
    31321 cgccattgat gctggccgaa tgttcggacg tattcatgcc atgacccgcc gcttccacgc
    31381 catacatggc aatatttttt tcattacgga aagggtagaa agtgcccatg gcgttgctgc
    31441 cgccccccac gcaggcgatc atcacgtcgg gatatcggcc ttcgaatttc atgatttgcc
    31501 ttttcgtttc tttgccgatg accgactgaa aatcgcgtac catccccgga taaggatgcg
    31561 gcccggccgt tgtgccgatc acgtaaaatg tgtcacgcac tgaagatacc cagtagcgca
    31621 tggcttcgtt catcgcgtct ttaagcgtgg cggtgccgct tttcaccggc acgacttccg
    31681 cgcccagggt cttcatccgg aaaacattgg gcgcctgccg gcggatgtcc tcttcgccca
    31741 taaatatttt gcattccatt ccgaagagcg ccgcgactgt cgccgtggcg acaccgtgct
    31801 gtcccgcgcc ggtttccgcg atgactttct tcttgcccat ccggcgcgcc agcagcgcct
    31861 gccccagcgt attgtttatc ttatgggagc cggtatggtt cagatcttcg cgcttcaggt
    31921 aaattttcgc gccgcgtaaa gattccgaca ttcttcttgc gcagtaaagt ggcgtgacgc
    31981 gtccggcata ttccctctga taccattcaa actctttctc gaagcccctg tccttttgtg
    32041 cgattttgta agccttttcc agctcgatta atgcgggcat cagcgtctcg gcagcatagc
    32101 gtccgccgaa aatgccgaaa tggccgtttt tgtcaggcaa tgtattcttc atgtgtccct
    32161 ctttttaaaa atggattgaa aattttcatg ctttgtatcg aaggcccgga tggtgtcaaa
    32221 gatacgggcc agcttctgat gatctttttt ccccggcgat gcttccactc cgctgttgat
    32281 gtccaaagcg tccggagtga ctgttttcaa agcgtcggca acattttcgg cgttgagtcc
    32341 tccggataaa atcagcggta ttttacccct gaggccagcg gccaagtccc agttggattt
    32401 tttaccggtg ccgccgtaac gtccggcatg acggttgtcc accaggatgg ccgccacagg
    32461 ataggcgcag gcgggggtta aatcaaattc gttctttaac tcaaccgcct taatcaatac
    32521 ctgcgacggg aaaagacggc aatcgtcggg cgattcgtct ccgtgcagtt gaatcatatc
    32581 gagtccgcag gtttcgacca gcttttttat atttttcggt tgttcattca cgaatactcc
    32641 caccttcaca accttatccg gcaaaacacg gataattttt tctgcatcat ccggttttat
    32701 atagcggggc gagggcggat aaaaaataaa gcccaacgca tccgcgccgc aatttactgc
    32761 agcgaaagca tctcctttac tggctatgcc gcagattttt acttgagtca cttcagctct
    32821 ctcctttcaa ctcgcgcagt ttcttgccga tgtccggcgc cgtgacgaga tgctcaccga
    32881 ttaaaaaggt cttaattccc gctttgatca ataattcaat atcttttctt ttccggatgc
    32941 cgctttccgc cacgacaagg ttgttttccg gaatccgctt tttgagtttg acgctggttt
    33001 cgatatccgt aataaatgtg tccagatcgc ggttattgat accgataatc tccgcgccgg
    33061 cggccagcgc taaatccagt tcttcctgag agtggacttc caccagttgg cttaagccaa
    33121 cttccccgga gagcgaaatg aaatcagcaa gtttttctcc taacacccgt acgataagca
    33181 aaacagcatc agctccgatg gctctcgtct cgtaaatctg aagaggatca acaataaaat
    33241 ccttgcgcaa tattggcaat tggacgattt gcctgatttg cgtcagataa tctttgtgtc
    33301 cggaaaaata cttttcatcg gtgagcatgg aaatcgccgc cgcaccattt tgttcataaa
    33361 tccgcgcaat cctcaaagga tcgaaatcag tgaccagccg gcctcgtgat ggtgaggcgc
    33421 acttgacttc cgcgataatg ttgcagtcgc tgctcgttaa cgcctttttg aaatcccggc
    33481 agggggcaaa tccggcaatt tcttttttca gttgcgcctc tgttgtttct tttttcagtt
    33541 gcgccacttc ttcttttttt gtttcaataa ttttgtcgag catttccacg tcgctctgct
    33601 cctgggatag tttccaatag cctttggtta ttggataatt cgatttgcga atttcagttc
    33661 acaacgtttc tggaattcga gtctttttac gttcagcaag tttcagtgtg cctatagtga
    33721 ccttcaggtt actttgcgcc tgggatagtt ccgctaacgc ttcgcgcgtc attgcattcg
    33781 cgctcagcga gtatcgctaa ctgttgctca tttcgatcag tccttgcagc tttttaacgg
    33841 ctgcgccgcc gtcaatgcag tcggttgcaa tcgccaaccc ctccttgatg tctttcgcct
    33901 tttctccggc aacaatcgcc agggcagcat tcagcaggac aacatgacgg cagggaccgt
    33961 ttttgcctga gagcacatcc cgggtaattt gggcattggc cgaaggatcg ccgccgcgaa
    34021 tggattccag ggaatccagc cgtccgaagt aatcaacagg attaatatta tatgtccgga
    34081 caatcccgtc acggagttcg gccacacgcg tctctccggt aatggtggct tcgtccagtc
    34141 cgtccagtcc gtgaacgaca aacgcttttt ttgtacccat gttttttaaa acatgcgcga
    34201 acatttctgt aagttttgca tcgtaaacgc caagcaactg ggctgtagct cccgccgggt
    34261 tggtcagcgg gcccagcata ttaaaaatgg ttcgaatgcc gatttcccgc cgcggaccga
    34321 tggcgtattt catcgcgccg tggagtttgg gggcaaatag aaatccgatg ccgatctgct
    34381 gaacacattc ttcaactatt tcctggtcgg cgctgatatt cacgccaagc gcttccagaa
    34441 catcggcgct gccgcagccg ctggaaacgg cccggttgcc gtgcttggcg acggtcagtc
    34501 ccgccgcagc tgcaacaaag gccgccgtcg tggaaatatt gaacgtattg agcttgtcgc
    34561 cgccggtgcc gcaggtgtcg atgatagttg aagaacaggc attaatacga gtggctttct
    34621 ggcgcatgat gcgtgctgcg cccgagactt cttccacggt ttctcccttc atgcgcagcg
    34681 ctgtgatcaa cgaggctatt tgcgcggggg tagcaactcc ctccatgatt tcatccatcg
    34741 cggccatcat ttccgcttcc ggcaaatccg ttttcatcgt ggctttttcg attgcttctt
    34801 taatcatgat cgtcgctcct tattatttcg tatatattaa aaaatttctg atgatgcgtt
    34861 ttccttgcgg tgtcagaacg gattccggat ggaactgaat gccttccacc ggatattttt
    34921 tgtgccgtaa tcccatgatc tcgccttcgt ccgtcagtgc gctgatttcc agacagtcag
    34981 gcagcgtttc cggatagaca atcagggaat gataccgccc ggcggggaaa ggattgggca
    35041 gtccggcaaa gatagtttga ccgtcgtgtt ttacatgaga tattttcccg tgcatgattc
    35101 ttccggcgcg cgatactgtg gcgccgaacg cgtaaccgat ggactgatgt cccaggcaga
    35161 tgcccatgat gggaacgtcc tgataaaatt tccgtataac ctcaaccgtt actcccgcct
    35221 cggcgggcga acagggaccg ggcgaaagaa aaatcgctct cggctgaaga cgcctgatgt
    35281 catccgtttt tattttgtca ttgcgataag tgttcacttt ttcgcccatc tgttccagat
    35341 actgaacgat gttgaatgta aaggaatcgt aattatcgat cattaaaatc atatttattt
    35401 ctcctgccgg ccgtttttaa accaaattgc tttcaaatat taagatatga atttttgaaa
    35461 gcttacatgg tcttatgcta atcatgcctg attggtactg atttcaaatc ctcctgctgc
    35521 aagctttatg gcctgcagca tcccgcgggc tttgttgagc gattcctgat gttccgattc
    35581 ggggatggaa tcatacacaa tgcccgctcc ggcctgaacg taaattctgc cgtttttcag
    35641 aatcattgtt ctgatggtaa tgcagaaatc catattgccg ctgaaactga agtaaccgac
    35701 ggctccgccg taaggtcccc gtttgacttt ctccagttcg ctgatgattt gcatcgctcg
    35761 cactttcggt gcaccggtta gggtgcctgc cggaaaggtt gctgaaagaa cgtcgaaagc
    35821 atctttgcct ttggtcaact gcgcgcgcac attggaaacc agatgcatga cgtgcgaata
    35881 tttctccacc atcatgaatt gattgacctg aacactgccg gtttgcgcca cgcgtcccag
    35941 atcgttgcgt cccagatcca ccagcatgac atgctccgca cgctctttct catcggacag
    36001 cagttcatcg gacagcgcgc ggtcttcctg ttccgttctg ccacgcggcc ttgtcccggc
    36061 aatcgggcgc aactccatgg tatcgtcctc tttgcggacc atcacctcgg gtgaagagcc
    36121 aatcagcgtc agatcgtcca tcttcaaaaa gaaaaggtac ggcgacggat tgatgaaacg
    36181 cagtgcgcgg tacaaatcaa tggagctgtt taaatattcg gtttcgaaac gctgagacag
    36241 cacgacctga atgatgtcgc ccgccgcaat atattccttg gctttttcaa tggcgtcttt
    36301 atactggtga caggtcatat tggattgaaa attgacgtca ccttctgttg ctgcggctat
    36361 cttgtgcgat gcggatgctg aaatgatttc aatcatttct tcaattttcc ggcatgatgc
    36421 tgcgtagcct gcttcaagcg attcctcatc ttcagtaaaa gtgcaggcaa caacttttat
    36481 ggtgtgacgc acgttgtcga aaatcatcag ggaatcgctg atgataaaaa cagcatcatc
    36541 cagattcaga tcatccggcg gactgggtgg aagttcttcg aaatagcgca ccatctcata
    36601 acccagatac cctaccgcgc cgccgtaaaa acgcggcagg ccgggaagcg ccaccggttt
    36661 atagcggttc agcaactcac ggagaaaatt caaaggaccg ccatgatggt ggcgggttgt
    36721 cacatttcct ttttcatcaa tgataactct atcgccctgg actctgaaaa ccacgctggc
    36781 gtccgttccc aaaaacgaat agcatcccca tttctcgccg ccctcgacgc tttccagaag
    36841 gaatacatgg tcttttttcc gaagtttcat taaaacggaa acaggcgttt ccacatcggc
    36901 taggatttcc cgatagaccg gaatcagatt tcctttttga gctaactttt caaattctgc
    36961 aaaattcggt tggtacataa aaattctccc taaaacgact aaagccgtga aatctattca
    37021 cggcttcaat ctgtagatac aaaaaagccg tgaaggtccc ggaggcactc cacggctttg
    37081 actttaatta aaaaggatca gcggcggaaa tccctccctt tgaggttcca ccaccagttg
    37141 ttaagcatta aggtttttac tgttgttttc ataaattaat ttccaatctc aatgtgggat
    37201 tcctataaca gcaagaaaat gacttgtcaa tagattttaa tgagactcgc cgaaaacgag
    37261 cgaagtgaag ttagaggcgc aagtcgaatt atccccgaag cgcagcggag agggattgtg
    37321 aattttaaaa gcataacaat ctgttagctt gcatcagaaa ggcagcggca cctggatcag
    37381 ggaggggaaa caacggcgac aggccttgtt ttgcatggaa cgggcgcggc acgcatccag
    37441 atcgataaaa taaccgtatc tttcgcacca aacattttcc cgttgcggtt ttactctggt
    37501 cgttgaacct cccgcgggtt tgagactgcg acgtattttt ttcatggcgg caaagatatg
    37561 agataaaagg gggcattgtc aacagaagat tattaatcct gcttgacacg tgtttgttta
    37621 tttttatata agttttccaa gccttgcaaa attggcgtat cgggaagaaa tttatgatgt
    37681 tactggaatc ctttttatcc gccgtgaaga gcatccgggt gcaggatgtt ctggatgtga
    37741 tgatcatcgc catcatgatc tttgctcttt tgacctggtt taaaacacgg gcgtcccgtt
    37801 tcgtactcat cggcatcata ctgttaggcg gcgtttatat cgcggcgcgc ttttttcagc
    37861 tttacctcac cactattgtt ctgcagagct tttttgctat tctgcttttt gttctggtgg
    37921 tcatttttca ggaagatctc cgtcgctttt ttgaaaggtt ggcgatgctg ggaagcctcg
    37981 gcaagaaatt tccggagatc agcggcttga aaagcacggc ggaaatcatt gcccagactg
    38041 cggcaaacct agccaagaaa aaaacaggag ctttgattgt tctgcggggc aatgaccccc
    38101 tggaaaggca catcaacgga ggaaccaaac tggatggcgt ggtcagcggg gctctgctgg
    38161 aaagcatttt tgatccgcat tccgtcggcc atgacggggc cgtcattata gaagacaaca
    38221 ggatcagtca attcggctgt catctgccgc tttccgccaa tacctcaaaa tattccaatt
    38281 tgggattgcg tcataccgcg gcactcggtc tttccgaacg atccgacgcc atatgcgttg
    38341 tggtatccga agaacgaggg accatttctc tggcctacat ggaaaatctt acggtggtgc
    38401 ccagcgcggc cgtcctgcat gaaacgctgg aacagtttta tatccgcaac acgccgacga
    38461 aaaaatcgca tcccgcgttg aactggctta aagaaaacac actggaaaaa gttattgcaa
    38521 tagcgatggc ctgtattttg tggatggcgt tcggctatca gagggacatt gtacggcggg
    38581 attttattgt tcccattgag tacaaaaata ttccgcgcaa ctggcagatc gatgaaatgc
    38641 aggtcaccga tgcccgggtc gtgctgaaag gtccggagca ggcgtttcgt ctgcttgacg
    38701 aacgctcttt gaaattatcc ttggatctat cgggtatcgc cgacaaaaaa cgcgaattca
    38761 acctgagcgc cgacatgatt aatgttccat ccaatctgac cattgcggat attgttcccg
    38821 ccaaaattca cgtaacggcc tccaaacgta ttccctggac attccctgtt catgtcaaca
    38881 ctcaaaatgc tctggcccat catctttcgc tgcagcggtt aagtgtagcg ccgtccaaag
    38941 ttcgggtttt gatcagtgca cagacggatc cggatcaaat caaaatagaa acggaaccgg
    39001 ttgatctaaa gagaatttta tccacgacaa cggttgatac aaaaatcgtg cttcccatcg
    39061 gtgttgcttt accggaggga caatcgccgg ctgcccgggt gcagattaag gtgaaacaaa
    39121 aggcttcttc ttaaatgagg cagacatgac gtccaagtat cacaaaagat ccagaaaaac
    39181 gggtcttgcg cccggaagtc tggtgcacat cggcagtccg tatgccgaaa aatcaaaaat
    39241 cactctcatg caatacaatg aaggtttttt tgaagcaaag gacctggaaa gcctgaagga
    39301 tttccaggcg gataaagata aagagatcat cacctggctg aatgttgacg gattaacgga
    39361 catacaatta ttcgaagaaa ttggcagaat tttcgggttg cacccgcttg ttctcgaaga
    39421 tattttaaat acggctcagc gtccgaaaat ggaagattac ggaggctata tttacctcgt
    39481 ggccagaaat tttcatgatc aggaaaacgg cagtttgctt ttcgagcaga tcagtatcat
    39541 tctggggaaa aatttcattc tctcttttcg ggaaaaagaa agcaatcttt tcaaatcaat
    39601 ccgggaaaaa atcagtcaga acaagggcag aatacgaaag gaaaacacag attatcttgc
    39661 ccacgccctg ctggataata ttgtcgataa ttattttgtt attctggaaa atctggagga
    39721 aaaaattgaa gctctcgaag acgatctgat cagacagccc gatagctcat ccctccaggc
    39781 aattcacatg ctcaaacgaa aactgattct gctgggcaaa tcgctgtggc ccctgcggga
    39841 agctatcagc gcactggaga ggtcggattc ggcgctgatt aacaaaacaa cggttgtcta
    39901 cttcaaggat atctatgatc acaccatcgc gatcatcgat accgtggaga cattcaagga
    39961 tatgctttcc ggtatcctgg atgtttatct ggataccgtc agcaaccggt tgaacgaagt
    40021 tatgaaaatt ctgacggtca ttgcgacaat tttcatgccc ctgacattca tcgccggaat
    40081 atacggtatg aatttcaagt acatgcccga actggacatg cgctgggggt attttggaat
    40141 tttaggcgtc atgctgcttg tcgcactgtt catggtgaga tattttagaa aaaagaactg
    40201 gttttaattt tgttgcattc aatcataaaa tatgcaaaat ttacccagaa tttatttaag
    40261 aatcaggagg caatgaagat gagtgttttt gcagcaagcg atatcgtgga agtcgctatc
    40321 agaattgaag aaaacggcgt taatttttat aaatttgccg aacagattgc caagaaagaa
    40381 gacgaaaaga agctttttgc cgggctggcg caggcggaag ttgctcataa gaaaacattt
    40441 gaaaaacttt ttgccggtat ggaaaaatcc aaccttcagg aaagttatga gggggaattc
    40501 ggcgaatatc tccgtgcgta cgttgataat aatttgatct tcaccaagga aataatggat
    40561 aaacagttgg ccaaagttac ggataccaca tctgccctgg aatttgccat tcagagggaa
    40621 ctggactcca ttctttatta tcacgaaatc aaaaaaatgg tccctgcggc acagcacgat
    40681 gcgctggaga agattatcga agaggagaga aagcattttc aggccctgac agccatgaaa
    40741 aaacgtttag cttgttagtc attcaactca actaaccata aaaaagccgc cctaaaaggc
    40801 ggctttttta tggttagcca atgaattttc acagcttttc ttcaggggaa aaagctttct
    40861 tatggcgttt tttgtttcgc ttaaaaacaa gatatcagca aaaataatca gagggctttg
    40921 cccttcccct aatccttttg aaaaatttca agaacaatac gcgcgtatga gaataaatct
    40981 ttaacgcaag gggttacaag ggcttgatag cagggcaggc atgaggatag cggctatgcc
    41041 gatttatcag tcgattcgtg cgaaatcttc tctggcagta ttttaatctg cgatatccct
    41101 agctcatttg ccaggtaaga tgccagagca taaataaata tcaacaaacc catcatttcc
    41161 aatgtttcct ctactataac aaaatataga taataatatt tatcctgtcc gtatatctcc
    41221 acatggcgca attccagcgc ttctattcct gcggcaccca gcataaaaat gataaaggaa
    41281 aaaacaaaca gatattttgt ttttttaggc aaatgaatca tgaaacgtaa ataaacgaga
    41341 gaaatcggtg caagaacaac taagtaaata agaacccatg cattagtagg gaaaagcacg
    41401 atctttatat taaacagagc taatatatga tctgaaattt cgtgaaagga aatgaattcg
    41461 tcgatcaata gaaaactgaa aattatcgcc aatcccaacc agtatctata acgggaggac
    41521 tgtgtgtttc tctcaaaaaa agcaattaag accagaaaaa tgacacaaag gaaccattcc
    41581 aaaatagtgt aaagcgtggg aatatttgcc tctccatcaa ggttaaaaaa cactgagaat
    41641 aaatcataac cgcgaaaaca tggaatgatg ttaaaaatag caagttgacc cagaatatgt
    41701 gccatgacta aaaaaaaaat tggcacaaaa atataaagga atattaattt tggtttcaat
    41761 tttatgatta tttgcgacag attctggtcg ttcgtatttg acatgcttca tttcatccct
    41821 tcagaccata tttcttgagt ttgtatcgca gcatccgttc cgatattccc aacagatcgg
    41881 cggcccgggt ctgattgttc tgggtttttt ccatagcctc aataattaat tttttttcca
    41941 attcatccag agagtctttt aacgcgccga aaggtttctt ttctgaatct tcaagcagga
    42001 aatcagattt gaacggcaga tcattggtgg taatgtattg ttcgcgtgcg atgacgacag
    42061 ccctctccag aatgttttct aattcccgaa cattgcccgg gtaatcatat tttaaaagta
    42121 aatccctggc ttcagccgta aatcctttta tgtttttgcc gttttcttta gcaaacttat
    42181 cgataaaata atcaatcaag acaggtaaat cttccttgcg ctctctgagc ggcggcagcg
    42241 acatcgtaac aacttttaaa cggtaaaata aatcatcccg gaaagttccc tctgccactt
    42301 gtatttccag gtcgcgattg gtggcgctga taaaccgcac gtcaacctgg agattgacat
    42361 tgctgcccag ccgctgaaat tcacgttctt gaagaaaccg caaaagcttg acctggatgg
    42421 tcgggctcag ctcccctatt tcatctaaaa agatcgtgcc tccgtcggcc tcttcaaacc
    42481 ggccgatgcg gcgcgaggac gctccggtaa aagcgccttt ttcatgaccg aacagctcgc
    42541 tttccagcag attttcatgg agtgcaacgc aattcacaat aataatcggt ttatcggcac
    42601 ggggactcaa ttgatgaatc agccgcgcca acaattcctt acccgtgccg ctttcgccct
    42661 gaatcaaaac ggaagctttg ctgggcgcta cgcggccggc cagattgata agttccgcca
    42721 tttgccggct ctgataaata attttatctg cagtaatgcc gtgtttgctc aagtcatcct
    42781 tcagcagcct gttctcctgg atgagttgtc gtcgctccgc tattctgttg accagaataa
    42841 tcagttcatc cagttccacg atcggtttgg tgatatagtc aatggcgccg gccttcaggg
    42901 catccactgc cgtctcgaca gtgccgtatg ccgtgatgat caccacatcg atttccggat
    42961 tgattcgttt gacttctttg agcacttcca gaccgttcat ccccggcatt ttgtaatcca
    43021 gaagaatcag atcgaaatgg ccgcccaaaa ccattttgat tgctttttct ccgttatccg
    43081 cttctgtgac cgtatggcct tcgcttcgga gaaaatcgcg taacagttcc cgctgggatt
    43141 tttcgtcgtc aacaatcaga atgctcagct tttgcatgaa atggtcatcc ttgtttgatt
    43201 atgaaacttc attcaatctt tcccggggaa gaatcacttc aaatgttgtg cctttattct
    43261 tccggctggt tacccggata tcaccgccgt gtccacggat gatttcaaag gccagtggaa
    43321 ttcccagccc cacccctttt tctttggtgg tgtattcggg attaaagatt ttttcgagtt
    43381 cttcttcggt catgccgcaa ccggaatcgg aaatttttac aacgatgtaa tttttgcctt
    43441 ccttgtccac cgaaatcaca attctgcctt tttccgccgt tatggattcc atggcgtttt
    43501 taatcagatt gataaatgcc tgctgaagtt tgttcacgtc catggagatt ggaacggact
    43561 cctgtttcca ggttgtttca agggtaattc cgcgcaacag ggcttcttct ttcatcaggc
    43621 taacgatttt ttgcagcagt tctgtgatgg aaaatttaca gaaatccagg cggcggcttt
    43681 tggaaaaatt aagaaattct tcaatgattc cattgaggcg tttaatctca tccctgatga
    43741 tgcccgataa attctgaaaa tcgtcaacct tttgagggtc ggaggggacg aaatctcttt
    43801 tcaatctctg ggtggccatg ctgatggcgt tcaatggatt gcgtatttca tgggctactc
    43861 cggcagccag ttgacccaga gaagaaagcc gttcagcttt ttccaattgg cgttccattt
    43921 ccacaatgcc ggccaggtgg cggttctggt catggtaaag cagccacatg gacaacaagg
    43981 cgatcaggac gataaatatc gagaaaacaa aaatatgttg tctattttcg acaagactct
    44041 tatccatgga gtttcggtca aggccgatgc gcacaatacc cacaattttt ttatccagat
    44101 acaacggcgc cgccaggtcg agaattttat tgtcggcata atccactttc cggctgacaa
    44161 tttttatttt tccggacaaa atttccttta gattgaagat tctgtgattt aaagcggggg
    44221 ccggctggcc gacactgctg agcagcttgt cccggtcatt aaagatttgc atataaacga
    44281 tgccgtggcc ttcgccgatc ttgtccattc ccctttcacc ggcaattttt aaaatccagt
    44341 aaacggatcc ggatttgtcc aggctaatga tcaccgctcc ggtattgtcc ttacggctta
    44401 atgcaataaa tcctactcct tgtttctctc ctattttcga tatcagttcc agcgtggtca
    44461 atttacgacc tttgtgcgta agctcgtttt cctcaaggag gtctctctgc aaagggccgc
    44521 tctgaaagac agcgtttcct tgagcattca acatccccac atacgaaaaa ttattgtccg
    44581 atgcgaattg acgcagggac tcatcggtca tgtccccttt tcgccattgt tcatccaaat
    44641 ctttggccag ttcggtaata gcctggataa cccattgttt agaataggat gcttcgtcac
    44701 gaccggacgt tttttcatgc ggagcgttct tatccggtac gccgataatg ctttttaaat
    44761 tctcttccgt taagcgctga agaacgccga tagtgctcaa agcctgattt tccataaagg
    44821 ctatcaggtt gctttcgctg cgtcgaagct cgagaaatcc tgtaatcaga attaatgcga
    44881 caaaaattgc actgacgata gccacggcca gaggttgaag gaaggggtca ttgtaattat
    44941 gagctttgcg atgaacgacg atgtcttttt ttctgaaaag acgcattgtt atccttaata
    45001 attgattttg ccggtcattt taagctattt gaaagaaaag tcaacgatta atcctttttt
    45061 atccgacaat tttgtcgaat gccggacaaa cagcgtcaaa aaaacttaat taattgtaat
    45121 tattatgatt tttaaaaatt aacaaaaatg attcgacatt ttttgccgct aaacaagtcg
    45181 cggatattcg ctgaagaagg cattaatata catattttgt caataatatt aatattatgt
    45241 aaaatattta gaattctggt acggcaaatg ctttaaatac gggcgggtaa attgtcgact
    45301 gaaaaataac gggtatgatt agccgacaac aaaataacct ggaaggtata aaaaccttca
    45361 ctcttcatgt ttaagggagg ctccgaccaa gcctccctta ctttttaatg acactcgctg
    45421 agaacgagct tagcgaagtt cgaagcgcaa gtggaattat ccccgaagcg tagcgtaacg
    45481 ggatttgaca ctcgctgata gtgaggactc gttgagaacg aatgaaatga tgttcgaagc
    45541 gttagccgaa ctataccagg cacgtagtgc cgtggtagaa caagcgaagt gcagtttgag
    45601 acataagtcg aatcaatgaa actccaattt caaaagcaga acgcattgaa attggtaagt
    45661 tgaattgtcc cttgtgcgga gcacatgggg atatcccaca tgcgaaactt agtgcgattc
    45721 taggcgcttt attctttttc cccttctttt ttcttatttc gccagtaatt gagtcgttct
    45781 ttaattgttt tttcaaagcc cacgcctttg ggctgataaa ataactctcc cttgatttct
    45841 tccggcaaat aatcctgcgg aacataactc tcttcgtagt catgagcgta tttgtaatct
    45901 ttgccgtagt tcaggtcttt cattaattta gtcggagcgt tccggatgtg taatggcacg
    45961 ggcaaatacc ctttggtatt gatggtattt ttaactttgc cgtaaccgac gtaaagagca
    46021 ttgcttttgg gcgctgtggc caggtaaaca gccgcctgag ccagggccag ttcgccttcc
    46081 ggcaggccga tgaagtgaaa tgcctgctgg gcggcaacgg cgacaaccag cgcctgagga
    46141 tcggcattac cgatgtcttc agaagcaaag cggatcatcc gccgcgcgat ataaagggga
    46201 tcttctccgg cggccagcat ccgccccagc cagtaaagag ctgcatccgg atcgctgccc
    46261 cgcaagctct tatgcagggc ggaaatcaaa ttgtaatgtt cttcgccgtc tttgtcgtat
    46321 aaaagcgatt ttttcagcag ggcatcctga gcgaatttca gcgatattgc cctctgatcg
    46381 tccggcaggt tttgtaccat ggataccagc acttcaagat tattcaacgc acttcgggca
    46441 tcgccgtcag ccagactgac cagatagcga agaacattgg catcaatttt cacatcgata
    46501 ttgccgaggc ctttttgctt gtttttaagg gcggcctcca gaatagcaag gagattttct
    46561 tctgaaaaag gttttaaaac cagcacccgt gtccgcgaga gtagaggagc gatgacttca
    46621 aaagagggat tttccgtggt tgcgccgatt aatgtgatca agccgctttc cacatgcggc
    46681 agaaaagcat cctgttgcgc tttattgaaa cggtgaattt catcgacaaa taagatggtc
    46741 ttgatgcttt ttctctccag caattctctg gcttcgtcaa tgaccgcgcg gatttccttg
    46801 acgccggaaa ggacggcgga gaaactgaca aattttgatt ttgtttcatt ggccagaatt
    46861 cgagccaatg tggttttgcc tgagccgggc ggcccccaga aaatcattga aaacagcttg
    46921 tcggattcga tcgcccgttt gagcagagat ccttcaccca gtagatgctt ctggccgatg
    46981 tattcgtaaa tattctgtgg tctcatccgc tcagccagcg gcgctgcgtc tttgacgttg
    47041 ccggaattcc cctgatcgaa taaatccaga gttttcattt ttttgctttt gccgcctcaa
    47101 tcagttctat caagcttgtt attttgtccg gagctttggt attgataaac ttgagccaac
    47161 gtttttcaga tagatgcccc ttcaaaaaag attctatatc gaaaacagga gaccagcagc
    47221 ggatgattcg aacacaggga agattgcctc cttcacgcag acagtaagca aaagtcattt
    47281 cgtctcctaa cttcgggcag cggatcattt tgttgtcgtg ttgtgttttc aaggggttca
    47341 gctttactga aaaaagaaag gccgtcaaga tataaatttc tcaacggcct tatctgttgt
    47401 tactcagggt ttactagggt ttggggaaat ttttaatggc ttttaatgct cgctcgatct
    47461 cgtgaccggg cagttcataa gctttcaatt tacctgcaaa ataagcatcg tacgcggcaa
    47521 gatcgatgat accgtggccg ctccagttaa agagaatggt tttttccttt ccttcctttt
    47581 tggcgcgcat cgcctcgtca acaaccgctg ccagtacgtg attggtttcc ggtgccggga
    47641 tgaaaccttc ggaacgggcc catttgacgc cggcgtcaaa tgtcttcaac tgatcgtagg
    47701 ctctgggttc gataagtttt tccttgacca gatgactgat gatcggcgcc atgccgtgat
    47761 agcgcagtcc gcccgcgtga atcggcgccg gcacatagtc atggccgagt gtgtacatgg
    47821 gcagaagcgg tgttttctga gcgagatcgc cgaagtcata tgcaaagggg cccttggtca
    47881 tggtcggaca ggaggacggt tccgcgccca ctattttcac atctttaccg tgaattttat
    47941 cacgcacaaa cggggcggcg atgccggcaa aattacttcc gccgccacag catcccataa
    48001 ccacgtccgg gtagatgccg agtttctgca tctgtttttc cgcttccagg ccgatgatgg
    48061 actgataaat caacacgtga ttcaacacgc tgcccagcgc gtaatgggtt ttgattttgc
    48121 tggtgaccgc atcttcgatg gcttcgctga tggcgatgcc gaggctaccg ggagaatcgg
    48181 gatacttggc cagaaaatct ctgcctgatt gcgtcagcgt gctgggactg ggaatgcatt
    48241 ccgcgcccca tgtgttcatc atcaggcgac ggtagggttt ctgttcgtag ctgattttaa
    48301 ccatgtatac acggcactcc agaccgaaca tttgcgtagc catggccagg gcactgcccc
    48361 actggccggc gccggtttcc gtcgtgattc tcttggttcc cgcgaccttg ttataataag
    48421 ccatcggtac ggcggcgttc ggcttgtgtg aacccgcggg actcacgcct tcgtttttat
    48481 aataaatttt gacggggcaa ttgagcagtt tttcgaaatt ataggcccgg taaagaggag
    48541 aaggacgcca caatagatat ttttgcagga cttcttccgg aattttgatc cagcgcttgt
    48601 tggaggcctc atgttcaata atgttcattg ggaaaattgc cgccagatcc tggggggtta
    48661 ccggttgacc ggttccggga tgcagtggcg gtttcatcgg cgtaggcaga tccgccatga
    48721 tgttatacca ctttttgggt atctcacggt cctgcaatac tacttttacc tgttccatat
    48781 tctttctcct tcaaataaag tattttgatc ctgaaattaa gaaagccacg ggattgtatt
    48841 cccatggctt taactattac cggagagtca tgggaagaag cttccctgcc catgctcata
    48901 ttttcccgag attatgggca gacatttgaa atctgccacc accagttgtt tttggaaacg
    48961 gttttgaaca tagtcggctc acgtgtttct taaagttgga aattccgtag cacagcgaat
    49021 aaatggatgt caagttgaaa ttttgacaac ttacacgtgg tttctcaact gaagctctga
    49081 aggatgcggg ttcagatata tctgttcctt aagataaggc tcgtcgtact tgcggacgta
    49141 gtggctgatc agcgtcaccg gcacaacaag gggaatatat ccgtcgcggt aagcatgaat
    49201 caattccagc agttctttct tttcatcgcc ggacaattgg tctttaaaat atcccatcat
    49261 gtgctgcaga acattgacgt gtttggccgg tgttgccttc accttaagcg attccagcag
    49321 caacgcctga tattcttttt gaatatccgg tccggcaaag tttttttgcg ccgccaccag
    49381 tttacccatc atttgataat gtttggtgct gtgcgcgagg atcagtaatt tatgtttggt
    49441 gtgaaaatcg atcaggctct ttttgaaatt tttcgcgctc aacgtttccc gccatctttt
    49501 tagcgcgaat attctttcaa taaagttttc gcgcaggccg ggatcgtgta gccggccttc
    49561 atcttccacg ggcaacagcg gaaaatgttt catgaacatt gcggcaaaca gtcccacgcc
    49621 gtttttcacc ggcatggttt tttcgttgta gatttttacc cgctccatgc cgctgctggg
    49681 tgaatcgctt ttaaaaatga aaccgcagag atcttctttt tccagttcgg tgacgcgttt
    49741 tttcgcccag gtttccatgc gttcggtcag atcctgtctg gtgctggtaa ccattaaccg
    49801 cggcgcgtcc ggatgccctt ccaggcgcat ggaaggtctg ggaatactca ggccgcattc
    49861 gacttccgga caaaccggca catagtccac gtattttccc agcgtatcgg ttaaaaacct
    49921 atccaattta tgtccgccgt catagcggac attgttgccc agtaaacagg agctgatacc
    49981 cagtctgatt ttttccatgt gaaatccttt gtagtgttat aatagtgaag cgggatacgc
    50041 tttaaactga atacttaaat tttaagtttt tcatctgctc tgagccgctg cgctaataat
    50101 gtcaacatca agatgtgatg agcgctacct ttacaatgaa ctgattcatc gtgcaagagc
    50161 cctgttgcat gatgcaagga caacgcatga atcaatcgag tcgaagaatc ccttttttta
    50221 acttgcgatg aatggtggac ggattgatgc ccagtaattt tgcggcgcca acaatgctgc
    50281 ccgtcatttg atacgcctgt ctgatccatt tctcctcaaa ctgatccatc gccttgggca
    50341 gggaaggcgg cagcacgggc atagcgggtt caggcgccgg aagagaccgg tcgctgctgg
    50401 cttgacgggg gttgatattc aggatcagtt ccagatcttc ctcagtcaac tcctgagatg
    50461 catgcatgat agccatccgc tcaataacat ttttcaactc ccgcacgttg cccggccatt
    50521 cataagtata cagtcgattc atcagcttcg ccggaagttt gatgtccgtt ttatattttg
    50581 tgttgaatat ctgaatgaaa tggaggatca gaggcgggat atcctcctgc ctgtcacgca
    50641 gcggcgggat gaaaaccggg atcacagcga tccggtaaaa gagatcctgc cggaactgtt
    50701 tgctgttcat gagttggtcc atggtgagat tggtggcgca aaggatgcga acatccaccg
    50761 cttgcgtctt gttgctgccg atcttctgga aatttccctc ctggaggaat cgcagtaatt
    50821 tactttgcat tttcaggggc aattcgccga tttcatccag caagagcgtt ccttcatgtg
    50881 cctcttccag gaggcccttc ttgccgctcc tttttgcacc ggtaaatgaa ccttcttcat
    50941 atccgaataa ttccgcttcc agaagttcat cgggaatgga ggcgcagttg atggcgacga
    51001 acggtttggc gctgcgggcg ctgagctgat ggattaaagc agccatgatt tctttgccga
    51061 cgcctgtttc tccccagatt aaaacggggg aagaagtggc ggccgctttc catcccaggc
    51121 ggatgacctg ctgcatgacg gaactggagt agataatctc cacctggcct gcgtccccgg
    51181 ccatctttaa gtttctgccc tcatactggg atatgatcgt ttcagaaatt ttgagctttt
    51241 tttccagagt cacgagtttt gtcatgtccc tgccggttcc gacggtcata acgagttcac
    51301 cccgttcatc gaagaccggg atataagtcc acgtagccgt ttgtccggtt tcgtaggtca
    51361 gattcatgtc aatttttttc ttgacttcca gggacttgag ggccgcggat tctctcagta
    51421 ttccgctggc cacgatgtcc ttcagattca tcccgacgag cgaccggccc atcaaatcgg
    51481 tcagtctgct gaaggtctcg ttgtatttca ggatgttggc gtcacgatca acgattaaaa
    51541 tagcctcgtc aatacaattt agcaggacat cgatatcttt catcagatat ttttgaatca
    51601 ggtccacttt tgctccttcg acacctttcc tataagcagt tctcttgcaa gttgcaacat
    51661 attttaacgt aaaaaagggg cagcatatta ataatccaat tatttctttg acttacattt
    51721 tggcattaaa tctgcaatat aaaacgatag aaaattttaa tatttacacc gaaaggagaa
    51781 aaattatgtc caatcaaaaa ccctggctga agttttacaa aaatgtgccg gaaacgatcg
    51841 attacccccg cgtgacgatg tacgaagccc tgatgcagac ggtcgaaaga tatccggacc
    51901 gcatcgctta tgattttttt gattacacat caacgtatcg ccagtttgcg caggatatcg
    51961 acatctgcgc caacgcgctg gcctcgctcg gcctgaagaa gggcgaccgg atgacgattt
    52021 ccatgccgac ctctccgcag ggtatcattt gcttttacgc cgccaataag ctcggcgtgg
    52081 tggccagtat gatccacccg ctctccacgc ccgccgaaat cgcctattat ctgaatgtca
    52141 gcaaaagccg ggttgccttg acgctggatt tgtttcataa cagtttcaaa aatcttccgg
    52201 aaaagacgcc gctggagctg ctgatcctca cggaaatttt tgaccagctt acgcaggcgc
    52261 agcccatggc ggaaaaaccc ttgccggaag agggaccggc tgtccggtgg tggaaggaac
    52321 tcatgactgc cgcgcacccg caggcgccga aagcggacat gcacacggat gaaatggccg
    52381 tgatccttta cagcggcggg acgactggct ttcccaaagg catcatgctc tccaacttca
    52441 attttatcag tgagggactg atggtggcca actggggagg cggcgaactg atggcggagg
    52501 attcttcggt tctggccatc ctgcccattt tccacggttt tggtttgggt gtttgctgca
    52561 acgccgcttt tatgggcgga agcaagagca ttcttgttcc ccagtttaca ccggagaacg
    52621 tagccgatct cattcgaaaa aaacgcccca gcattattgt cggggtgccg acactttacg
    52681 atgccctctc caagaatgaa gtattccaga ccacggacct gagctgtctg aagaatacat
    52741 cctcgggcgc cgattccctg ccgcggaccg tcaaggacag gtttgaagaa gtcgtgaaga
    52801 gacagaatgg aaacgtccag ctggtcgaag gctacggtct gacggaagcc gtcaccgcga
    52861 ttatgtccac gccgattgac ggataccggg aaggcagcgt gggcattcca ttccctgata
    52921 tgctggcgaa gatagtcggg atcggcacca tcgacgaggt tcctgtcggc caggaaggag
    52981 agctttgctt gcacggaccg gccgtcatgc tgggctacct ggagcagccg gaagagaccg
    53041 ccagtgttct caaaacacat gctgacggca aggtctggct tcacacgggg gacattttca
    53101 ccatggatgc agacggttat ttctattata aactgcgtct caaacgcatg atcaagtctt
    53161 cgggcatgaa tgtctatccg gttcaggtgg aagcttgtct ggatcaacat ccggatgtgg
    53221 cggcagtctg cgtgatcggc gtgccggatg aggctcaggt ggagaaggtc aaagccttcg
    53281 ttgtcctgaa ggacgctacc aaggcatctg ccgaaatgga aaaagaactg attaactttt
    53341 cccgcaagca tctgattaag tggtcctgcc cccgggaagt ggagtttacg gacaagctgc
    53401 cgctgacgaa agtcggcaag attgcctaca ccgaactggc aaagcaggaa attgaaaaac
    53461 tccgcaaagc gggaaaatat acaggaggaa aataatgaac catctggagg aatatcgccg
    53521 ggcggtctcg gacccttccg attatgtcca acaactgaaa aaggcatcgg ggaagaaaat
    53581 catcggatac acgtgctctt acacacctga ggaagttatc atggccgcgg gcgctcatcc
    53641 cctaaggctt ttcggcacaa aacagcatat cagcctggcc gattcccact tgcagtctta
    53701 ctgttgctcc gtggtccgcg gaattctgga ggaaggtctg tcacgacggg ttgactacct
    53761 ggacggcatg gtttttccgc atacctgcga ctccatgcaa cggctgtccg acatctggcg
    53821 gatgaacatc cccttcggtt ttcatctgga tatcaccctg cccgttaaac tcgatacggc
    53881 aagcgccaaa gattatatga tcgacgtctt aaaaaaattc cgggatgact tgggcgggaa
    53941 actgaacgtc gaaatttccg atgatgcggt gcaaaaagcg atcgcgacca ccaacgctat
    54001 ccgtcaatcg gttcgggcga tctatgacat ccgcaacctt catccccggc tcatgccggg
    54061 agaggatcta tacaacattg tccgcgcgtc catgatcatg gatcgggacc ggctggccgt
    54121 gatgctggcc gaaacagccg ccgaattgaa agaacgcgcg accagtgcca agcccgcctc
    54181 cgtaaagcgc atcctgatgg cgggcggcat gtgcaatcag cccgacgtct atgccatgat
    54241 cgaagaatcg ggaggcgaga ttgtctggga tgatttctgc acgggggcac gctattttac
    54301 cggtttgact gacgccggaa gcgatccgct ttcgagcatt ggcgaacgac ttctgaatcg
    54361 ggtggtctgt ccggcaaagc atgccgacct tgatggtcgc gcccgacata ttatccagct
    54421 ggcccaggaa aaaaacgcgc aaggggtcat tttcctgttg cccaagttct gcgatcccca
    54481 tgccttcgac tacccctacc tcaaagagcg tctcgaaaaa gccggcattc cgctgatgaa
    54541 cgtggaagtc gatgattccc tgcccgccga agggcagttg aagactcgct ttgaatcttt
    54601 tttagagatg ctgtgaaagg agataacatg tcagatacac aagataccga aaagaaaacc
    54661 gtcagctaca caaaacagat gcgggatatt atgaccgcct attatattga cgcaaaaacg
    54721 gcccgggaaa ataataaaaa agtggcctgg atcaccagcg gcggtccggt cgagccgttg
    54781 attgccatgg atatcattcc cgtttacccg gaaaattacg gagccatgat cggtgccagc
    54841 aagatgggcg tcgatttatg caagaaggcg gaggacatgg gctattcgag cgatctttgt
    54901 tcttacgccc gcggcgacat ttcctgtgcg accgtcaaag gcggccctat tggcggcctg
    54961 ccggaaccgg acatgttggt atgctgcaat aacatctgcg gcaccgttct caaatggtat
    55021 gaagtgcagg cccggtattt caaggtgccc ctgttcattt ttgatacccc cattgtccac
    55081 acggaattca cgcgggagat gaaaacctac gtgcagcggc agatggaaga atatattgtt
    55141 ttcctggaaa atgtttccgg ccgcaagatg gatcgggacc gcatggtgga ggtcggcaag
    55201 ctctccgtcg aggggcagca gctctggcag gaggttttag atacgactat gaatagaccc
    55261 tctcccatga ccgccttcga tgccttcttt tatctcgccc tgatcgtcac gctccgggga
    55321 acgaagcagg ctgttgactt ctaccgggga ctccgggacg aaatgcgcat acgggccagc
    55381 gaaggaaaag gggccattcc ccgcgagcga taccgcctgc tgtgggacaa cctgccgatt
    55441 tggtaccggc tcaaatggct gtctcaggaa tttgccaagc acgacgcctg cctggtcgct
    55501 gacacctaca cctcggcctg gtgcggttct atgaaataca ttgaccggaa tgattttatt
    55561 ggctccgcgg cggaaaccta cacacgcatt tatctgaata tcggcgtgga caagatggcc
    55621 gagatagtcc tttcgatgat tgacaaatac caggcggatg gaatcgtcat gcactccaac
    55681 cgtagctgca aaccttattc cttcggtcag tatgacatcc agaaaatcgt tgagcgcgaa
    55741 cggggcattc cctcattgat aattgaagcc gatatgtgcg atgagcgaag cttttccgag
    55801 gcgcagattc aaacgcggat cgacgccttt atggaaatga tccgggcgaa aaaaggggca
    55861 tcctgaaatg tgcactgccg ggattgcaag accttgccca ttagggtaaa gataatatgc
    55921 cgtacccagt gacacacggg attctctcgg caacaccaaa aaaaaattac gggggtatat
    55981 gtcaatgaaa gttcgaaaga ttatcttagc tgttatctgc acactgatgc ttgccgcaac
    56041 agctgtcatg gcaagtggcc catcgttgca gggagaagaa ctctacaagt acctttcaaa
    56101 aaaaaccatc agcgaaggag atgccgcgtt cgtggaaggg ctgaacacaa gctcgctcaa
    56161 gctcgactgg tctgcggcgc aaaagaagga tctgaccggc aacctccgca tcttgaaagt
    56221 taagaacgcg aaggtcggga ccgaggcgct ctattttata cgcggcagcg aaggagatat
    56281 gtacgtcgta tcactgccgg ccgaaggcgg gcgccccgcg gccgagtatg agaaaatctc
    56341 ggaaaacagg atgatgttcg cgctcgaaac cgttaccgga gaacacgaag gcacgaccta
    56401 tgccttcgcc cggatcagcg gggagccgaa ggctattctc ttcgaccgca tattcaagat
    56461 agccatcgtc ctcatgctct tctttgttat ggtcggtatg ggactgaccc tgaagctcag
    56521 tgacttcgcc ctcgtcttcc gcaagccgct gggcatcgtt atcggaatag tcctccagta
    56581 cggcctgatg cccctggtcg cgttcggcct gagctatatg ttcggattct accaggccta
    56641 tccattcata ttcatggggc ttatcctggc gacggcgagc cccgcgggag tcacctcgaa
    56701 ccttctgaca caattcgcaa agggcgatct ggcgctttcc atttccctca cctcgatctc
    56761 gacgatcctg gccatgttct gcacgccgct cctcctctcc ctttacggga acaccagttc
    56821 gggagcatcg gttccggccc ttctcatcgc acagaccatc atcgtgctgg tcatcgttcc
    56881 actggccgta ggcatgacgg tgcgggccaa attcgagaag gccgcgctca aggcggctcc
    56941 gatcttctcc atcctaggaa ttatcacggt cctgttcatc atggtgacgg gcgtggtggg
    57001 aaatctcaac gcgctgaaag acaccgctcg ctacagcctc tccttctacc tcgctcccct
    57061 gattttagct ctcaccggca tggtgatcgc gatagtcgtg gcgaagattt tccgtgtgtc
    57121 cccgaagcag gtccggtctc tcgctttcga gactggcgtc cgcaactccg ccctttccat
    57181 gacgctcgcg atcctcattc aggaccagat cggcgatttc tatagctcgc tctttatcgt
    57241 caacggggtc tatggtctgg aaatgtacat cgcgggtata cttttcgtga tgttcttcag
    57301 gaaattttcg aaggaaacgg aagggtagtc atctcactct cggtttcttt ttcgagagag
    57361 ggcaagggaa tcccccgaaa gttgtgccag cgttaattag agtgtggaac acaaaaacac
    57421 tttaaggagg cacagatgaa gaagcgattt acagaagagc agatcatcgg ggttttgcag
    57481 gaagccgaac gaggggagaa agtcaaggac gtatgccgca aatgcagctt agccggacgt
    57541 ctgtttttac ttgaccagat gagtttctta tactactttt aacacaagta aattttcatt
    57601 acgtcaagca ctgaaggcca gtaataaaat aattcaacag agaacctttg aagaaaaaaa
    57661 ttacaagaat cggaggtaaa aatgaaggac gtaacttttc tgacccataa gggcgtaaga
    57721 attttgtatg aagatcttga aaatgccaaa caggaggacg tattaccctg gatcgaaaga
    57781 gctaaaggag ttattcgcag tcagcctgaa aaatctgttc ttgctctgct caatgtcaaa
    57841 aatgcaaaat ttgacacaga agtaacagcc gccctgaagg aattcgcgaa gggaaacgaa
    57901 ccttatgtca aattcgctgc tgtttacggt atagaaggtt tgaaggaaat aatattcaaa
    57961 agcgtcctga ccttcaccgg caggaagaac cttgttcttt gtaagaccct tgaagaagca
    58021 aaagatattc tggcgagcaa agtttgaaac aagcgaaatg atttttgtcc ctgccaccag
    58081 aaagtggcag ggtaattccc tataagattt tccgatcact gcgtatcaga aaatcaaggt
    58141 tcattaaaac ctaagttgaa tgggttcttg tgcaaagcac atggggatat tccgagaacc
    58201 aagtaccctg aataacctaa atgtaaacag gggagaattc ataacgacga gatatttctt
    58261 aagagttggc ctagcaataa agaaaacttt ttggagtgaa gattatcaaa gcgcaaaaac
    58321 ttgcctattt tgacacatcg catgagatgc cgctggaaat tatcatggcg gccggattta
    58381 ccccgtataa aattctggga gatgtccacc aatccaatga cccagcggat aaacatttat
    58441 ccaacttttt ctgtggcgcc gcaaagagct ggctcacgga agctctggcc cgctccaaag
    58501 aatgggccgg cattattttc gcgcaggggt gcaacaatac caaccggcat tatgacattt
    58561 ggaaggttca tgtccaaacg cctttcctgc actggttcgg cgctcctctg ctggacagtg
    58621 acgccgcgaa aaaatattac cgggttgaac tgcaaagact gattagccat ctggaaaatc
    58681 aatttaatat aaaaattacg cctgaaaaaa taagagaggc gattatcaaa tccaacgaaa
    58741 tcaagaaaaa gctgcaacgt ctctctgcgc tcagagcaca gaaggacatt cccaaccgcg
    58801 attatcttgc cgcgatgata aaatgtctta cgaatcccgt ggatgaagtt tcggcttggc
    58861 ttgactcaga gatagcggcc tggaattcaa agccctcttt tccgtccggc aaaaaacaat
    58921 ttttattaac cggcagcgac atcacgtatg tggaatggat ggacacgctg gaagattgcg
    58981 gaatacggat tgttcgtgac gacctttccg taggagaaag atttttcgcg acactgattc
    59041 ccgacaagag cgatccgctg gatgccctta ttgaatatta catgaacatc cccaagcccg
    59101 cgacaaggcc gaccattcag aaacggttgg actatcttta taaagctctc ggtgagacaa
    59161 aggttgacgg tgttctttcg caaaatgtga aattctgcga gccttttgcc tatgattccg
    59221 tgcccgtcaa taatgccctg aaagacaagg gctacaaggt gatccacctg gaacgagact
    59281 tttctcctgc ggccgaccag caactcgcca accggcttat ggcctttgtg gaaatgatgg
    59341 aggtagggaa caatggctaa aacaaacaat tctgctgaga atgacagaaa gataaaactc
    59401 aaaaccctgc ctcacgcgaa agacctgaag tggccgatgc tgtggtggtt taccatgggg
    59461 tcaatatacc atgctttgcc ttggaaaaag acggcctgga catgtgccgc ctttcccatt
    59521 gagctcatgt ggatttacga tatttttccc gatcaccctg aaaatatggc aaccgtggcg
    59581 gccgcccgca agcagtccca gcgtctgatt gagcatgccg agggaatggg ttattcacgt
    59641 gacctgtgtt cctactgcaa gacaagcatt gccgcctacg ataccaaaat caaaaagacg
    59701 ctcggcggaa tcagcaagcc cgatatcgtg gtgtgcacca atgccatctg cgatacccac
    59761 tggaagtggt ttcagattat ggccgataaa atgaacgtcc cgttttttat gtttgatatt
    59821 cccaaatggg tgagcggtac ggatgagaaa accctccagg gtaatattga ttacgtggta
    59881 gagcagtttt acgacctgat tgtatttatt gaaaagcaca ccggtaaaaa aatggatgag
    59941 aaaagactgt acaaagtttt ggataaatcg acggaactgt ccgacctgtg gcgggaaatt
    60001 tttgaacata gaaaggcggt gccgtcgccc tacagcggag cggagacatc ggcgtcattt
    60061 tttcccctgg tggttctgcc gggtgttcag gtcggcataa attttttcaa aaaaattctt
    60121 gccgatatca aaaacctcac cgcaaagggc gagggaacca tgcccaaaga caaagaaaaa
    60181 taccgtgtga tgtgggaagg cattcccttc tggtaccgga tgaggttcat gtatgacctc
    60241 gcgcaatacg gcgcggtcat taccttcgag ccttacacct actcgttcgc tcccgaaaag
    60301 aaaaagtgta acaccatgga agagacattg cgcgatgtcg ccaaactcat catggacacg
    60361 ccctacgcct acaacctcga aagacgtata gagcattttg aaaaatggat tgacgaatac
    60421 aaaattgacg gcgtcattct gcacgaaaac atgtcgtgca ggccatccag cgccggcatg
    60481 gtcgatctgg cggaggccat catgagggac aagggcattc ccgtgctgat cctccaatgt
    60541 gacatgaacg atccccgggc ctactccgaa gggcaaatta aaacgagggt ggaggggttt
    60601 attgaactca tggaggcgaa taaaggcaag taaatagaac cgttgtcttt tgctctgcag
    60661 ttcagccaag tctttttgac ttggctgtcg taaatatcgt gaactgataa aaggtcgagt
    60721 ccaaaagact cgaccaaact aggggaaaaa ttgtcactga ataaacactt ataatatcca
    60781 accaaaggaa taagaggtta gatattaaga aaggatataa tgattacagc aggaattgat
    60841 atcggttcta ttaccacgaa ggcggctatt tttgccgatg gtaagattac ggcgacaaaa
    60901 gttattttta ccggatacaa tgccgaagcg gcaggcctca aggtttatga agaagccctg
    60961 aaagaggcgg gcattgataa aaatgccgtg gcaaaaattg tttccaccgg ctacggccgc
    61021 aacagcgtta aattcgctga taagtcctac acggaaatca tgtgccacgc caccggagca
    61081 cattttttga atccgcatat ccgttccatc attgatatcg gcggccagga cagcaaagcc
    61141 attcttctgg acgacaaagg caaggtaaaa aactttgtca tgaatgataa atgcgccgcc
    61201 ggaacaggcc gttttctgga agttatggcc agagccatgg aagtcaatct cgatgaattc
    61261 ggcgctatgt ccatcaattc aaatcaaccc gccaaaatca gcagcctctg cacggtattt
    61321 gcggaatcgg aagtgatttc cctgattgca aaaggtgaac aacggaagga ca
//
LOCUS       DAOOLB010000003        59642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_332_length_59642_cov_40.711363, whole genome shotgun sequence.
ACCESSION   DAOOLB010000003 DAOOLB010000000
VERSION     DAOOLB010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 59642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59642
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_332_length_59642_cov_40.711363"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="PLF77_00715"
     CDS             complement(<1..1144)
                     /locus_tag="PLF77_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07403.1"
                     /translation="MTEESKCPVTGMMKKPISGGGKSNRDWWPNQLNLKILHQQNKKS
                     NPMGEAFDYAKEFKKLDLKAVKKDLITLMTDSQDWWPADWGHYGGLFIRMAWHSAGTY
                     RMGDGRGGAGTGNQRLAPLNSWPDNVNLDKARRLLWPIKQKYGKKISWADLMILAGNC
                     ALESMGFKTFGFAGGRVDVWETEEDIYWGSEEEWLATSDKPKSRYSGERDLENPLAAV
                     QMGLIYVNPEGPDGNPDPIASGRDVRETFARMAMNDEETVALVAGGHTFGKCHGAGPA
                     THVGPEPEAAPLEAQGLGWGSSYKSGKGGDTISSGLEGAWKPNPTTWDMGYLKVLFKY
                     EWELVKSPAGANQWLAKNVEDEDMVADAHDPSKKRRPMMTTADLSLR"
     gene            complement(1309..1737)
                     /locus_tag="PLF77_00720"
     CDS             complement(1309..1737)
                     /locus_tag="PLF77_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HPC07404.1"
                     /translation="MIYKDGLIGQLKEKGLKITPQRLAIIDALVEHSEAHPGATLIYE
                     AASKKARRISLSTVYATLKEFSENGLIRQLEFDRMENRYDVDLSDHVNLICNRCGKIF
                     DYAIPASLEPRDIARKSGFVVTETRLEFHGYCRDCLKRSD"
     gene            1884..2213
                     /locus_tag="PLF77_00725"
     CDS             1884..2213
                     /locus_tag="PLF77_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07405.1"
                     /translation="MPVYLQVILLVAIFIATVIIFLYIGGLAVRRASFQIIAEMEEAR
                     AFKEGRAIKIQDERKNFFRVGTKNIRPRALNLLIADGLVIKTNNGKYYLDKEKLAQAK
                     AQLSSKT"
     gene            complement(2235..3380)
                     /locus_tag="PLF77_00730"
     CDS             complement(2235..3380)
                     /locus_tag="PLF77_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07406.1"
                     /translation="MNRKTELEEITHTGGKVNFNIKIDADGRIAYNVGLTHSRPTPAA
                     LFAVYAIPQGVAVHDIKMGGIGTPWNPPPLPDCFPVFISSDSTGMFGHQCPSCNGYWR
                     AGLGGKICPYCAFTADEYYHFLTDAQQRYVRQYCEVLSNALASGQSGEHIIDMDSVAE
                     AAGKDCEKPAFYYAEERQQNLFTCNACGKVNDVLGTYAYCSSCGTRNDLQELEKNIQQ
                     IRDRINAGGPYETCVKETVAIFDSFAGHYAKQLLTRVPLTPARKAWIEKSHFHNIGTV
                     SEMFRKVFDIDVLSSIDSEDVSFGTLMFHRRHVYEHKGGEADEKYITDSGDSVRLKQA
                     LRETKETAHRTANFITKIATNLHKGFHEIFPPLEEPIKQYEQRKYKR"
     gene            complement(3652..5532)
                     /gene="mnmG"
                     /locus_tag="PLF77_00735"
     CDS             complement(3652..5532)
                     /gene="mnmG"
                     /locus_tag="PLF77_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HPC07407.1"
                     /translation="MTNLTDNYDVAIVGGGHAGCEAALACARMGCRTILFNINLDSIA
                     LMSCNPAIGGLAKGQLVKEIDALGGEMGKITDKTAVHFRLLNASKGPAVHSSRMQCDK
                     QLYRLTMKAVVENQPNLYVRQAMIEKLVIEDGKVIGVLDQSEHFYGAKKIIITTGTFL
                     NGLVHVGPSQTPSGRAGELASIGLANHLKELGFDIGRMKTGTPPRLRASSIDFSRLER
                     QDSDPDPRPFSFTTEKLRTERLPSYFSATSAETHRVIRENIQHSPLYSGIIKGVSARY
                     CPSLEDKVMRFAERQSHPVVLEHEGLDTQEVYAKGLGNCLPLELQYKIVRSVKGLEQA
                     QIMRPAYAIEYDFVQPTQLKPTLETKLVDGLYLAGQINGTSGYEEAAAQGLWAGINAA
                     CAVQGAPAFLLDRSEAYMAVMIDDLVTRGVDEPYRMFTSRAEYRLILREDNATIRLMG
                     KGCELGLIDKTHYLELQDKIKKIENGIEQLKASPVKPTAETNEKLATLNSPPLSNHTT
                     LHGLLKRHELSYDNLSIFPGWEPELDAFVKQQIEIETKYEGYIKRQFDAVKKLKDQEK
                     KKIPPDFDYNSVPGLSNELKLKLTKIAPQTLGQMERIPGMTEGAISAVLIMMKKHELQ
                     GR"
     gene            5733..6686
                     /locus_tag="PLF77_00740"
     CDS             5733..6686
                     /locus_tag="PLF77_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418923.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPC07408.1"
                     /translation="MPKPDFWKNNKNYYDLKSYWRNLFGCSVHKLQIDAGFTCPNRDG
                     HVALGGCIYCDGRGSKLRQAGELPSVTEQIASGKKYYTKQAGKFIAYFQTFTNTYAPV
                     EKLRALYDEALNQEDVIGLSIGTRPDCIGPHVVELLSGYAQKYHVWVELGLQSVHDKT
                     LTLINRGHSFQQFEDAVNALAGHGLNICVHIIIGLPGESDEDVLATAKTLAAMPIHGI
                     KIHSLLALDGTALGEMYKKGRINMITKEKYVSLVADVLEVLPPQTVIQRLTADGYRDI
                     FLAPDWAMNKLDVLNSIDKELIRRDSYQGKKYRTNRAESLD"
     gene            complement(6727..6933)
                     /locus_tag="PLF77_00745"
     CDS             complement(6727..6933)
                     /locus_tag="PLF77_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07409.1"
                     /translation="MEEKERMIDRLFNPMLDALEDHPYSGMLVIAFVCILIVAFFPPS
                     LVFILIFIGYGFYLNSKRSKKDKP"
     gene            complement(7291..8064)
                     /locus_tag="PLF77_00750"
     CDS             complement(7291..8064)
                     /locus_tag="PLF77_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07410.1"
                     /translation="MMKKMFLSSLVVFIAASIVSSPPVCFSEQPDPKVWKHLSDDFYY
                     NKINLIQSADSISVWTYRTIADDERKYLIEHFRESDPKKSIKYQKLDHQTILLKMDCR
                     NKQSKIEKLVNYDDNESVLYEETYNNSEWTGIIPGSKPEEIYHDICVTSERQEQLHAL
                     KMKCEKDCDAWVRVDEKKFFRDPFTHQSHYNTRLDKCFILLNYTKKPLKVLREMKGNK
                     IYGAVRSQKNGKTITCNVLEKTCKSEAEWDAHVKPYMEE"
     gene            complement(8276..10093)
                     /locus_tag="PLF77_00755"
     CDS             complement(8276..10093)
                     /locus_tag="PLF77_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPC07411.1"
                     /translation="MTENDIKTLPQGLKWLVDKQPDRVAMRMKDYGIWHDISWRQYYE
                     NVKKVAVGLYALGVKPFDHVAIVGENKPEWLFSAMGVMSLGGIFVGVYTTNPTAECEY
                     VVGHSESVVFFCEDEEQFDKALAFRERTPRLKKIIVWDMEGLKHFDDPMLMSFEKLLE
                     LGEKIDKERPGLYEKIVAGRESEDTASIIYTSGTTGPPKGAQISHQNYLWIARQSQKI
                     TKLSVDDETISFLPLNHVYEQIFDLMMHLTTGHIVNFTENTDTVMNDLRDVSPTLFHA
                     VPRIWEKYHSGIVLKMADATWLKRTVFNLAFKIGTTYNGFKLAGKPIPWYLFLGYQLA
                     YFSVFWKLKQRLGFDKVRLGFSGAAPISHEVLKFFQSIGIPIREGYGLTETTGITHMS
                     DEEHFKVGTVGRALPDSEVKIAEDGEILIKHGGIFQGYFKEPEATAEALEGGWFHTGD
                     VGEIDAEGYLKITDRKKDLIITAGGKNIAPQYIENLLKFSPYITDAVVIGDRRKYITA
                     LIVIDEENVVKYAQDHKIQYTTFASLTRAEEVIRLISDEVEKANRQIARVENIRKFHL
                     LDKKLYTEDGEVTPTMKVKRKSISAQYKDIIEAMYTEDP"
     gene            complement(10103..10873)
                     /locus_tag="PLF77_00760"
     CDS             complement(10103..10873)
                     /locus_tag="PLF77_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC07412.1"
                     /translation="MLSVKNIETWYDLIRALHGISFEIKEGSIVALLGSNGAGKTTTL
                     KTIMHLLDDDQPEKGSIEFLGKRIDRMDTEEIVRLGISYVPEGREVFPELTVMENILM
                     GAYTRKDRKGIQDDIDGILKQFPILSERKNQQAGHLSGGEQQMLAIGRALMSRPKLLM
                     LDEPSLGLSPLLTKEIFSIIQNINQKGVTIFLVEQNVNMALKYSHYAFLMENGRIVRA
                     DRPEILREDDDVKEFYLGIATETSVKGFKRYRRKVRFR"
     gene            complement(10873..11646)
                     /locus_tag="PLF77_00765"
     CDS             complement(10873..11646)
                     /locus_tag="PLF77_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC07413.1"
                     /translation="MAHFKVENLNISFGGIRAVSDVSFEVEYNKIFSIIGPNGSGKTT
                     IFNIISGIYKPHAGEVFLDNENLVGLRPDTIARKGVARTFQNIQLFGNATVMDNLMLG
                     RHIHMKTGITSGAFMWGKRSFAAKEELKHREIVEHIIDFLDLQSLRDLFVSSLPYGKR
                     KLVELGRALALEPKVLLLDEPSAGMNTEEKDDLRIWIKDIQEDYKVTILLIEHDMNMV
                     MGISDRILAINQGVKIIEGTPQDVQKNPEVIAAYLGEEE"
     gene            complement(11732..12922)
                     /locus_tag="PLF77_00770"
     CDS             complement(11732..12922)
                     /locus_tag="PLF77_00770"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPC07414.1"
                     /translation="MKRFIIALTIVIAVFIASLLVFAQAKSGFDNKEIRIAQWGPQTG
                     PAAPWGSVARGSGLLFQIVNDEGGIHGRKIKYFIRDDQYNPAQTKAVVKELVEREGIF
                     AFVGGVGAACGMAVKDYLAQNKIIWVNPSTAIDEYVFPVNPYLFAMYTLYDDEASILT
                     KYTVEKLKAKKIGFLYQNDAYGKSGLKGCKARLNTYKMKLVEEISVEPGEKDLSSQML
                     RLKNSGAEVTFLWVNPTSAAIALKTAATIGFKPQWISSDTLMDFPLMNKITGGLWEGV
                     ITGGIGLPTDVDHPLMNKYRAAAKKYAPEERFGVFYTAGIMFAEPLVDALKRVGPKLS
                     TEALLRELNKTKNFQGLGAVVNWSPTVHQGCDSIQILKCGPNSSYIILQDWTSNELAT
                     WKKK"
     gene            complement(12991..13440)
                     /locus_tag="PLF77_00775"
     CDS             complement(12991..13440)
                     /locus_tag="PLF77_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07415.1"
                     /translation="MSKTVKILILACAVLVVFGLVIWDAPRNRLELFLEKSVIEETAR
                     NYLTAEMKKDSRQVYALLAPSSDYKKTHTYEEYLKDIAENSSAALNTCKIVRIYHLRD
                     NHNRHNYPAVDKLVQVEVEVTFGHAGPNSIFNYSFTFLKEKGKWYKG"
     gene            complement(13463..14335)
                     /locus_tag="PLF77_00780"
     CDS             complement(13463..14335)
                     /locus_tag="PLF77_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPC07416.1"
                     /translation="MFEMLLIEGIAIGACYGLFGVAVAIIYKTSDVINFAQGEMGMVA
                     TFVAYHVLTVYNVPFWLAFPVTLIFAILLGMAIEFCFLRPAKDPTVLGLLIITLGAEM
                     LLMGLAGWKWGAEQKDFPFLLSVTKTHEMLPGIFINEWTIGVFSLSIIIMISLFLFFR
                     YSKLGIAMRATQQNVNAAKIMGIKVERVFSITWAMSSLIGAVAAMFFAARSVLDPNFM
                     MEPFMRAFAAAVLGGLTSIPGVVVGGLILGIIENFFGYAWPEWKPIVAFVVIILIITV
                     RPSGLFAKHYIKKV"
     gene            complement(14357..15427)
                     /locus_tag="PLF77_00785"
     CDS             complement(14357..15427)
                     /locus_tag="PLF77_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPC07417.1"
                     /translation="MDMKRDYYEDVELFRSGTILTWSIVLLVFLFTLPVYLPSYNIYL
                     LNIIMVNIILAVGLNILVGYTGQISLGHAGFFAIGAYGTSLIMMNFHFPFFAAIILAA
                     LIAAFFGFIIGLPALRLEGPYLAIATLGFGLTIMHIIGRWDFFGGRMGITAPPLDMGI
                     SGTSFSYVIQGDVKIYYLILMMTVLLVIGARNLMKTRVGRAFMSIRDDDIAAEVMGVN
                     LTIYKTLAFAVSAFYAGVAGGLYGFVVGFFDPFTFNLILSIIFLVMITVGGLGSIMGA
                     IMGAMLITYLQYDLLKNVENLPYLGALLTTVSKKWFTVIGLANFSSIALGLIMITIVI
                     FEPLGMFGIWIRIKKYWKTWPF"
     gene            15655..16281
                     /gene="hisH"
                     /locus_tag="PLF77_00790"
     CDS             15655..16281
                     /gene="hisH"
                     /locus_tag="PLF77_00790"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HPC07418.1"
                     /translation="MIAIIDYNAGNITSVARALQSIGQEFIITDDAKKLDAASRVIFP
                     GVGAAGEAMAYLRQKKLDVWLRDYIKTGKPLLGICLGTQIILDYSEENDTPCVSLVAG
                     STRRFPETLAFNGELLKIPHMGWNSIRLLRSHPVFDGVSPPAEFYFVHSYYPAPADQE
                     VVLGVTDYGMTFCSVLARNNLVAMQFHPEKSGRPGLQILKNFCTWRLS"
     gene            16278..17057
                     /gene="hisF"
                     /locus_tag="PLF77_00795"
     CDS             16278..17057
                     /gene="hisF"
                     /locus_tag="PLF77_00795"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPC07419.1"
                     /translation="MISKRIIPCLDVRDGKLTKGIKFKGNVDIGDPVEAAREYYEQGA
                     DEIVFYDITASSDKRNIMIDVVRRVAETIFIPFSVGGGIRNLDDMRNVIHAGAEKVSV
                     NSAAVENPQIIMQGAKAFGSQCVVLGMDVKQVDVSDAIPSGYEIVVHGGRIYTGIDAL
                     WWATEAEKLGAGEICLNSIDADGVQTGYELNLTRLISENVHIPVIASGGAGKPEHLAD
                     VLTKGKADAALIASMVHYRTYTIAEIKHYLSEKQIKTRLFW"
     gene            17136..>17239
                     /locus_tag="PLF77_00800"
     CDS             17136..>17239
                     /locus_tag="PLF77_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPC07420.1"
                     /translation="MDKKRYELNVQLAQMLKGGVIMDVTNVEQAKIAE"
     assembly_gap    17240..17339
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17340..17818
                     /locus_tag="PLF77_00805"
     CDS             <17340..17818
                     /locus_tag="PLF77_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HPC07421.1"
                     /translation="RRIAEGAAMIRTKGEAGTGNVVEAVRHMRAMNREIGQLVQMPVE
                     EVTGFCKTNGIPYEHALKVKELSRLPVVNFAAGGIATPADAALMMQLGCDGIFVGSGI
                     FKSADPARRARAIVKATAYFNDPQKVLEASMDLGEAMPGLEISQIPPEQILAGRGW"
     gene            17825..18514
                     /gene="pdxT"
                     /locus_tag="PLF77_00810"
     CDS             17825..18514
                     /gene="pdxT"
                     /locus_tag="PLF77_00810"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HPC07422.1"
                     /translation="MSLRLVSDKPSVIGVLDLQGGVHEHLEHLERLGVACKRVKQTED
                     FKDLAGLIIPGGESSCLSRLLTIFGMKDVLLAAHRRGMKIWGTCAGAILLAQNVVGED
                     ACLKLIDITVERNSFGSQLDSFAGEALIPGISADPVPLTFIRAPKILAAQPDVRVLLK
                     IDDYIAAAENDSVLVTVFHPELTGCLALHQYFAVKCGLKTATGQAGHSDPDWDISSWT
                     KYAALAGRAGD"
     gene            18637..19743
                     /locus_tag="PLF77_00815"
     CDS             18637..19743
                     /locus_tag="PLF77_00815"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPC07423.1"
                     /translation="MQNIFSELKTDSRFIRFVMKVGSPSPLDPDEQHRRIPAVFVLMI
                     ISVATAVSGIYHCSSGKNPLVVLDVIGFILSLIMLLYLRQGKNPGIAYGMISTGFVLL
                     CCAATVLGRTEMSLIFWALVLPAGCFSILGRKMGMFVAAFFFFINLLLMNLPEHMILS
                     KPFPASVAARFSVVYLILTFMLYYYESSHQMLIRYIRHEENKFQNASRYDPLTGLSNR
                     RDILEKMENERERHSRLEKTFTLIMGDIDNFKMLNDTLGHEAGDYVLQTIGRILKNQV
                     RGIDCSSRWGDDEFLILLVDTDLEGGQRVAERIRKKVENTSFQYQGKGLAVTMTFGLS
                     IYQSADEQIDACVRRSDDALEEGKRQGKNRVVPV"
     gene            complement(19804..21168)
                     /gene="mgtE"
                     /locus_tag="PLF77_00820"
     CDS             complement(19804..21168)
                     /gene="mgtE"
                     /locus_tag="PLF77_00820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPC07424.1"
                     /translation="MQKVKKKEPTALETLKDLISRNREFDIIDLFSDMHPADIADLID
                     ELEEEDRLHLFSLLDVDKASDVILELSDTSREQLIEDLSNEKLTDIIEEMDSDDKADI
                     IAELSDDQAKAVLEAIEPEESQEVQELLKYADDTAGGIMQSELVSVRKDATINDAFQA
                     VADAKDEIENIHNIFVVDETDKLIGTVPLQSIITTKRFTPILDIIDEDIPSVLHDMDQ
                     EEVARLFKKYDLVSIGVVDNQQRLLGRITIDDVVDAIQEETSEDIYRIAGLGEDDTVF
                     NNTTESVKKRLPWLYLNLITALASVLVIGFFEETIKLMVALVFFMPVVAGLGGNAGSQ
                     TLTLIVRGMALGEITFENAKRALFRQIAVGIANGIAVGIVIGIIAYFWKGIPILGLIL
                     GLAMIISIFTGTLVGALIPLALKRLKLDPALGSHIFLTAFTDAFGFLTFLGLATIFLK
                     IFHL"
     gene            21464..22294
                     /locus_tag="PLF77_00825"
     CDS             21464..22294
                     /locus_tag="PLF77_00825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC07425.1"
                     /translation="MTCLYSLREFYFLGAKGTILIKADNIEKNFFSVGRYWGNLNASL
                     KRKESVWWMNTGVAVSDMNWAWNETPLAAPDTAIVSEIREDYKKNNLRFWWWVYPCGQ
                     SAATSAILQNAGFRLYANVCCMAADLNESFPEPYATGDMEIYHVRSREDLAVWEDVAF
                     HGFEMPRHARDPFGAFVSSFETGDQFPQRFILGCIQGKPVATSLLFKDQRTAGIYYVS
                     TLPSYRNRGCGLSVTQAAMQLARESGFKHVVLQATPMGKRVYERLNFKRYYHAGIYTL
                     "
     gene            22432..23322
                     /locus_tag="PLF77_00830"
     CDS             22432..23322
                     /locus_tag="PLF77_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07426.1"
                     /translation="MKRNSGHNIACDNECGGLHQSNPLSLAKNEISSTLSWFMTDNAL
                     YPGVDKHSSPLRYENWSGRYSEYISRQEAESIVSRFICPEKDENFTLFFVSDYKDTYL
                     GKNFLKDGSDIYLKHYRMTDAPREIRLRNVLRRCLARKNFNAFYQLHQKNIEAVKPLF
                     YVMKRGRWIPDEVIAVSKGASGNKTIETLMADSRDNDEFRLLAVNLGSYVAELQSREI
                     LFKELYRNLIAVNQTTHWDFILCDLDEIRSVSEKRFYKHRKHVEKLRKKIKRYGDSFL
                     HAFDQGYQTRMHDQTAPSRR"
     gene            complement(23382..23726)
                     /locus_tag="PLF77_00835"
     CDS             complement(23382..23726)
                     /locus_tag="PLF77_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HPC07427.1"
                     /translation="MGKMTQCPWGSFEVLSETAGHKVKRIIVEPGGGLSLQLHHFRSE
                     HWFVVAGTGDVTVGDKIISLSAGGSVDIPAKVKHRVLNHADGHLVIIEVQTGARCDDG
                     DIERFEDLYGRI"
     gene            23879..24565
                     /locus_tag="PLF77_00840"
     CDS             23879..24565
                     /locus_tag="PLF77_00840"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HPC07428.1"
                     /translation="MNISYTVRRSRKRQKTMSLQVCDDAKIVVSAPSFTPADDIRRFV
                     EEKQGWVAKILARQKQEAAHAKEKKYETGEQFFYLGQLYPLEVFFEPFEKEGIAFRNH
                     RFYLNCRSDKVLRKQCFVAWYSKKADEMIRRRVDFFSGMLKLPYERIRITSAGSRWGS
                     CSEDNSLSFSYRLIMAPPDVVDYVIVHELVHIREKNHSSTFWQKVEAVMPSFREQRRW
                     LQDNHSNLAL"
     gene            24599..25456
                     /locus_tag="PLF77_00845"
     CDS             24599..25456
                     /locus_tag="PLF77_00845"
                     /inference="COORDINATES: protein motif:HMM:NF021445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HPC07429.1"
                     /translation="MEHIEIIDLKHRDGLAERFKKMNLRISDYTFANVYLFRNISHYE
                     FVTKECGLFVSGWNKQNQNYVMPLNDPRGCDVKTFAEILGARNFFFPIPEEWLVYFPK
                     DKFSVSYDESESDYLYLTENMAGFKGKQYLRHRNHLKQFLASYIPDGVTLSSHNCNDA
                     MIVLHQWQEDSRTASDKTDFVQCSEALQNFSGLALWGIIYYIAGKPAGFIIGEALAGD
                     TFCLHFAKASKRYHGIYEYMFNDTAKRLESAYKYLNLEEDMGNKNLRRTKSSYGPEFL
                     LKKYRVGII"
     gene            25457..27712
                     /gene="feoB"
                     /locus_tag="PLF77_00850"
     CDS             25457..27712
                     /gene="feoB"
                     /locus_tag="PLF77_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPC07430.1"
                     /translation="MEKKTAMSESVNITSLQEGDQGVIHAIEGGSAITSRFAGMGIVS
                     NARFRIAQISGGLIVLQVANTRIALGRGEASKITVSKVAPEADVCMPPIEKEISVALV
                     GQPNVGKSTVFNILTGLSQHVGNWPGKTVEKKEGTHRADSVLIRIVDLPGTYSLTAFS
                     EEERITRDFIIKEQPGLVVLVLNATALERGLYLLAEVLLLNRPVIVVLNMLDVAADQD
                     IEINIRALEDSLGVPVIPMVAKRNSGIRELVSHISSIASSEYKFQPVKPEVSTDHQLI
                     YQQILTLVRPYIHDPYTPEWVAVKLMEGDPEVTQLVEQQVQTIARDQIEKLLIKHEDA
                     LHAVVNGRYDWIEEITRSVVSRFKMGQVVLTDRIDHVLTRPVFGIPILLAVMALVFFL
                     TYSVGVPLQEWLSDRIQQFIGFAEPLTTVWPSWLSGLLLNGVIGGVGSVLTFLPVLLI
                     FFAIMAFLEDVGYMARAAFVMDKIMHLVGLHGKSFIPMCLGFGCNVPAVLGARIIETK
                     KARMITLLLIPFVPCTARLAVLTLVSAAIFGQNGAFVAWSLVALNIAVLGIAGVFVNK
                     TLWKQDAPFIMELPIYHTPDAKTIMMVVWTRTLSFIRKAGTVILAVSLIVWMLSYFPN
                     GNVEESLLASFGRLLQPLGIPLGLDWKMITALLTGLVAKENVVATLGVLYSVGRDGLS
                     NMLPAIMSPESAAAFLVVMMLFIPCAATIAVLKKEMNNNKWFYTAIVMTLLVSYVGGI
                     AAYNFVKWLGV"
     gene            complement(27717..28889)
                     /locus_tag="PLF77_00855"
     CDS             complement(27717..28889)
                     /locus_tag="PLF77_00855"
                     /inference="COORDINATES: protein motif:HMM:NF025182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4185 domain-containing protein"
                     /protein_id="HPC07431.1"
                     /translation="MNKNILIRNVCRGFIFLFVVIPLLSCSTFTAARYDADVSPGHCL
                     PVFPDRDGWYGGDGAYSIKLDKDRTLWVFGDTFVGCPGCRRDRIDMDIVLGTTVAISS
                     CPENRGFEITYYLQHSGGKYVSSFGKNEWLWPQDPFMVNRVLYIPLISIVPGDKEDEV
                     FNFKVAGHKFVRVKDFSATDPRNWNYDYVDLTHALPPEIAAFAATSVVHDRYVFFYPF
                     YVHVKNDLNVFGNILARIPVDRIDRPAGAIEYYTKEGTWENRLSPEKVKIVLDASVSE
                     LSVRYHEDDQKWIAVYLSTQNKGDRLLYQTSDKLEGPWSEAKPLGAPVPEVDPRSALY
                     DKGNFCYAGKEHIGFSRGKYLVVTYVCNSSEDMEKQDSFIRRNMFLYRPVVREIGY"
     gene            complement(28894..29451)
                     /locus_tag="PLF77_00860"
     CDS             complement(28894..29451)
                     /locus_tag="PLF77_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HPC07432.1"
                     /translation="MGFITIFLIATGLGLDAFSVAIGIGAAHQKRSWIPVLRLAIAFG
                     FFQFIMPIIGWMAGLTVVEVIASFDHWIAFGLLALIGGKMILEGCGKGIDETQSDRTT
                     GWPLLMLSIATSIDALAVGFSFSVLKHPILFPAVVIGVVCFVMTATGMIFGKALAKLF
                     GRKVEIFGGIVLIVIGVKILFDHLM"
     gene            complement(29461..>30262)
                     /locus_tag="PLF77_00865"
     CDS             complement(29461..>30262)
                     /locus_tag="PLF77_00865"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPC07433.1"
                     /translation="ALLQLANDNARNLWESTYKKREEKEFALQILQEKLSLAGLPRRI
                     ECYDISNIGGTYAVGSMAVLRDGDPDQNSYRRYRVTAPAEPDDYKMMQEVLSRRFSSG
                     EHLPDLVVVDGGKGQLNVALSVLKDLKIRTDVIGLAKEKCRRCPVRAGQKTTRTWEDR
                     VYLPGRKDALYLSAWPQALRILQLVRDEAHRFALRYHHQIKHKGDLHSVLDDIADIGA
                     KRRKILLSHFGSAGKIKDATKEELQNVPGIGKVLAEKIFNALKIIQNE"
     assembly_gap    30263..30362
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<30363..31126)
                     /locus_tag="PLF77_00870"
     CDS             complement(<30363..31126)
                     /locus_tag="PLF77_00870"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HPC07434.1"
                     /translation="MMKENNVLKDKIALFPRSPGVYLMKDPAGKIIYIGKANHLKSRV
                     ASYFTGKDSRPMTPFLMARVYDIEFINTATAKEALILENNLIKRHRPRYNVTLRDDKT
                     YYYLSLDPTDKFPRLQLVRKRLHNAALYFGPYPSGLAAKETLRFVQQIFPLRSCRNRD
                     FKLRPRPCLEYQIGRCLAPCSGRINEEAYRKLADNAVSFLRGRSRELITELKKQMNDA
                     SEELHYEEAARLRDRIAALEHALEKQNVERGEEKDQ"
     gene            complement(31187..31582)
                     /locus_tag="PLF77_00875"
     CDS             complement(31187..31582)
                     /locus_tag="PLF77_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07435.1"
                     /translation="MKYLIITGAVLILLAITTGWFIIAGKYLSLTVMKKIIRDEEKLI
                     KAHVDYVIMGILLLVLFATGINLPLVVAVLACAGALANPSLFLFLAVKPNVNKKAGSF
                     FGIVSTLVFLITTAGIGGAALLLIQELIQ"
     gene            complement(31623..32663)
                     /locus_tag="PLF77_00880"
     CDS             complement(31623..32663)
                     /locus_tag="PLF77_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07436.1"
                     /translation="MSRRLKGFLFILCFLLISFLVAELFFRYLYPKIAPFDDRIDIRY
                     ANPYTMFSRFAYGSQEPAKTRGEFRIFMLGGSTVEYGNPPLPVYVEQELKRRGFENVR
                     VFNYGVISQNSGQELAHLVFHVLDSEPDMVVFYDGGNDIMDPLLYDPRPGYPFNFLAY
                     ENNVLFQATGDYPWPALLAYGSEMLRHVFRNYFFEKFGNFSNLRLSAQHGEYAWSEKI
                     AGIYAGNLAKAGQICRAYHVEYVAFLQPIIFYKRFAVSDTEKNILKGWPPEEAGIVRP
                     YTNDVRKRILRNIREEQKRFPFEFHDLSDIFCNSRDAVFTDYIHVNDTSNKKIALAIC
                     DRLMDNIKNSHP"
     gene            complement(32736..34550)
                     /locus_tag="PLF77_00885"
     CDS             complement(32736..34550)
                     /locus_tag="PLF77_00885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC07437.1"
                     /translation="MSVNYFIVYNPLTYQLMDLMPANSITPFDVITYLSGQNAHEMNA
                     ENYLRFIDFQPEKKRATMVDALAIEMYAGIYIQHQLAKLAQDHLVVMVDGKRRNFSEV
                     IRQKKAPPQAVFISTISSSFPAAAAAAIVLNHAKIPVIIGGIHVSTMPEDADTFIRKY
                     APYPELISIVRGAGDSTVLKTILNDLKTHSLKSDYSGETLIENGVWGNFENLEPLEPL
                     KINLLNRLPVIKYTPLKDFRINPVAPFTGCIFSCKFCSISTLPQRNKIVQFRDTDDFV
                     EELRSYQKETVDFKNRYYFFTPDNLFSNKKNLISFLKKIIASDLRINYAVQISIEVAD
                     DEELLKLIRASGGTHFFIGLESLDIRNLKHINKHIVKDIEKSGMSVKKYYASKIRKIH
                     AHGISVHGSFIVGLPYDYFNSPTDNTGVDITKFCIRNRIGAQPSTIVDLPGSVFFHES
                     QENNTFIYGKKGTMDYFLSLSVADLAETNKIPPDSLHQSPLIIASMVYEISKIVGAPV
                     RATFNSSIIFFRSLLHPTNKGQGLHFWQRFIDAVCSAVSQTAVALYKEQGESLVRSKH
                     GVRGIMERLYDTEKNPEIRKMFKNYIPQFTEYGTSGKT"
     gene            complement(34801..35433)
                     /locus_tag="PLF77_00890"
     CDS             complement(34801..35433)
                     /locus_tag="PLF77_00890"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HPC07438.1"
                     /translation="MPVRTYHLILLATVLIVWIWSAIKPQDTYLTWFLETLPFMMALP
                     IILLTYKKFRLTDLTYTLIALHAMILMLGAHYSYAKVPLGLWMEEWFGWTRNNYDKIG
                     HLMQGFGPAIYAREIIARTSPVQKGKWLAFFSLAVPLAFSALYEILEWAASLSNPNDT
                     EAFLGTQGYLWDTQTDMFLCLIGSMASLLLLATLHDKHLLKTQAPGINKK"
     gene            35612..37594
                     /locus_tag="PLF77_00895"
     CDS             35612..37594
                     /locus_tag="PLF77_00895"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPC07439.1"
                     /translation="MKKIFLQFFLLLVLFGLLPVAGIFNPVAAEESARNASGNQTEID
                     LMLLFTNDLHSYFLPHRVLTEDGRQTVQGGYARLACMINEQRRLHPAKTLLVDAGDFS
                     MGTLFHTAFMTEAFELRLMGKMGYAATTFGNHDFDFHPEGLAKMLTAARSKSQILPQI
                     VASNLIVNGDHPGDKTLKQALNDYPVRDYMVLERNGLRIGLFGIMGKDAADDAPFAKP
                     VMFADPVEASKKTVAILKNREKADLVICLSHSGTSADAKKSEDEILARAVPQIDVIVS
                     GHTHTVLKTPIIIGRTIIVSGGRYGRYLGSLGIRFDRFKGASLASYDLRHVSSEIPED
                     KAVQEAIAGYEKIVNRDFLAHYDLTFEDVIAESDFSMESLESMHRHPRETGLGNLIVD
                     AYREAVKQAEGPDYRHVHFALQPLGVIRDSLLKGKITVSDVFRVLSLGIGPDRIPGYP
                     LVSFYISGKELKDILEVHTTIAPMHKKDAYLQVSGVKFTYNPRRIWFDRVTSVWVADE
                     KGVYQPLDQTKLYRACANLYTAALIDYVSSVSFGLIHVVPKDSSGKRLTDLNSSIVSM
                     KKTGEIKEWLALALYLRSFQEPQHLQTAKIPLKYSKPEGRYLAQPSCNPVRIFSKGNI
                     LTKVFLVSVILLLMICGLIVYVFVKKMKKISKKNPR"
     gene            37845..38591
                     /locus_tag="PLF77_00900"
     CDS             37845..38591
                     /locus_tag="PLF77_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPC07440.1"
                     /translation="MQIELTPVEKMKAPEQIAERLLKYILGGGVSPGEKLPPERTLAT
                     QLNVTRATLREALKKLEQLKLIVIHQGKGIIVEDFHKASIDLIFSLLVINGQIDLKIL
                     ENILEARELFGTDVARCAARRADKKDVEQMKILMNELIKATDPGKLQQLDFEFFRQLA
                     LASKNMVYILLMNTIKTIHDNHLDLFLPLSTNLDTTLQQEILQAVIDGDEEKAAEKAN
                     TFLKAGMELLKFLKSLSDTKESQNADRETN"
     gene            38569..41874
                     /locus_tag="PLF77_00905"
     CDS             38569..41874
                     /locus_tag="PLF77_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPC07441.1"
                     /translation="MQTARQIDKDPIEQTQKLNVLKIQRTCVHDGPGIRTTIFFQGCG
                     LRCVWCQNPEALSLKPMPSPAVHYAIADVLEVVLRDKDYYLKTGGGVTLSGGEPLLQD
                     PESLTHLLKKLKDEKIHIAVETSLHVPWENISKTAPYIDLFLVDLKVVGDEDLHRKCT
                     KQDSRLIHDNIRKLLASKANIKFRMVMVPEYNDHEQHIKATADFLKSVNHKSIELLKY
                     HNMYEDKAKRLGLVRESLNISPEQSLTAVKKAVELLKPLDIYAECEDLGNFRSKAVFT
                     DRVHAIQKDIRKSGYHLCFETSKLKTKFYRKNGFKKPTHIHRAERLDYVLKNKQVRIY
                     PHELLVGNFTSKRVGGQVWEEHYGILFSSILHQIHRQTPVAFQCSWKDRLYFYCRILP
                     FWQKHCLLTKVNKTLSDLVLSISRLCEMNVGFNNNMAAIAHFIVNFERILTLGTTGII
                     KELEEAQKEKPQNDQTFYQGAIIAVKALENFALRYADALSDMSKTEADPVRRKELEDL
                     VQICRHVPKHPARTYHEALQSMMFLQVALCIESYENAISFGRLDQILYPYYKKDKEAG
                     IITYEKARELLALFILKMDEAILVNDGDTYFAIGRLFETQSTDQTVTFGGVDKDGQDA
                     TNDLTYMLIDICELQFLAVDMSARIHPNSPERYLNRIAEVYINGSPQPKLNNDEVYIQ
                     SLSKHYDTSIENIRNYSIVGCVEPNASDDHFGNTDCANMNVVLPLLQALKGEEDDLWN
                     YGFSDQMEKLTTKFIEYNFRGGNRVSKWIADKYGKARASYKTKKKTAYNPPANMDELL
                     ERYQNRLNHLASSILADHQKIEKEICKNFTTPLASALSRGCVEKGLDVNQGGCEFNTS
                     GIQAVGITDAADSLHALNEVVFKKKMYSLMDVIHAIDNDFEGDDGQQIRQALLAVPKL
                     GHDDSPEAVKWVNKTLEIYCNALRQVPNSPRNGFYTAGYYALNVSDVYGKKTPSLPSG
                     RLKGVPLANSISPHYGMQMSDLLSSLNSVSGVDFVKYAPNGTTVTFTMDSALFQGRDG
                     ITNLAGIFSTYFKKGGMQFQPNVINREMLLDAYNHPEKYPYLLVRIAGYCAYFNHLSD
                     DLKKVIINRTCYS"
     gene            complement(41953..42600)
                     /locus_tag="PLF77_00910"
     CDS             complement(41953..42600)
                     /locus_tag="PLF77_00910"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPC07442.1"
                     /translation="MLKKYRYVLTGFLQDFLYYFIRGYSWTFRLQVVNEKPWMDHLKQ
                     GGRILICCWHQQFFSAIRHFKTYAPYRPALMISQSKDGDIIARIAEKTGWHTVRGSSS
                     KDGSRALRGMIDYLIKSRFAGHVVDGPRGPAGIVKAGVVKLAQASGALVVPFYISADR
                     AWYFNSWDHFMLPKPFAHVTLRFGDMLDLASGESEEDFERQRLRLEGIMKPNLHL"
     gene            complement(42743..43210)
                     /locus_tag="PLF77_00915"
     CDS             complement(42743..43210)
                     /locus_tag="PLF77_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07443.1"
                     /translation="MKTIKIYCIVILAALLLSAYGSLAADWGFRGLLPRGTGYYDKNS
                     IRKISDHIYQVWTVTIYNERGRADAYAMLLKQEKAPGNPAVLNQESILLELDCLQGKY
                     RIVSINIYDEKDTLLFSVPETNDGWHDIIPNSINEKLNNAVCKAVDNHRDGNP"
     gene            complement(43296..44177)
                     /locus_tag="PLF77_00920"
     CDS             complement(43296..44177)
                     /locus_tag="PLF77_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC07444.1"
                     /translation="MKEETEINIPKEFTNKLFWQSLNPMSITRATDGMYVDVNESFTE
                     FFGRRREEVIGNTAADIAYATSLERLKILEEINEKGFARNITITVRDKKGDLRCALVN
                     TLPVEIKNQVYWLTVGTDISDLNPAAKISHEDALIKSLNSIKGAGVVLIRNHQKKQPS
                     LCYMNEEAKMVLKTHSLDNLLRTLAENDSTYLSIGAKYYHVKNINNHRRSPLKLIVME
                     RMPDTICIREKLTILDLTPRQKEIAFLAATGYSNREIAGRFCITEYTVKDHLKKIFHT
                     LGVKKRSEISPKLFHWR"
     gene            44586..44978
                     /locus_tag="PLF77_00925"
     CDS             44586..44978
                     /locus_tag="PLF77_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07445.1"
                     /translation="MKKRIMVLLSIIVFVLGLVCIQGCQQNENPKFQNEYQAVLLTTG
                     QVFFGKVEFVGTDYVLLKDVFYIRSVANPDTKQVSNTLIRKGNEIHGADQTYINARHI
                     IMIEPVSPQSPLAGRIKELQSQKPQGQP"
     gene            complement(45093..46277)
                     /gene="metK"
                     /locus_tag="PLF77_00930"
     CDS             complement(45093..46277)
                     /gene="metK"
                     /locus_tag="PLF77_00930"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPC07446.1"
                     /translation="MSKLHTRHIYTSESVSEGHPDKVCDQISDAILDACLEQDKHAHV
                     GCETAVGPNLVVNLGEITCKNWNKINPQAISRQVVQDIGYDDPCENFSWDTFNYLSAI
                     HGQSPDIKQGVDRDRPENQGAGDQGMMFGYASYATPQMMPAPIYYAHLLLEELTILRK
                     ANVIDYLRPDSKSQVSVLHEDGKAVKITTVVISHQTLDVPLKRIRKDLIDVAHQVLDK
                     TGMLTNKTEFFINPTGRFVIGGPAGDSGVTGRKIIVDSYGGVGSHGGGAFSGKDPSKV
                     DRSAAYYARYAAKNIVAARLADKCEIQIAYAIGVAKPLSINVCTYGTSVIPENKIEKI
                     LTEGKIFDFRPAMMIKDLGLTTPKGWSYRKACNYGHFGRKGFPWEKTDRVDALRQAAG
                     LK"
     assembly_gap    46784..46883
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<46884..47440)
                     /locus_tag="PLF77_00935"
     CDS             complement(<46884..47440)
                     /locus_tag="PLF77_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07447.1"
                     /translation="MKKLMSGIIFFAGVLFAPFSVSAGVSVHIDIPLPPPIIFPAPPH
                     LVVIPETYIYAVPDIQDDLFFYSGWWWRPWNGRWYRSRYYDRGWTYDHQPPFFHRTIP
                     PGWKDNYRNKTWKGHRWEHRKISHHELQRNWSGWKKEKRWEKHSWGVKNWKDRRSGTQ
                     YRREVKKSKTVIKYRDDRRPVREDRR"
     gene            complement(47595..48536)
                     /locus_tag="PLF77_00940"
     CDS             complement(47595..48536)
                     /locus_tag="PLF77_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPC07448.1"
                     /translation="MRLLKAIVVLIILCLATGIGFVYLAPEQATSLALDVERKRSGLE
                     KKEINLSDHIRYVYLEGGKGEPLMLLHGFGANKDNFTRVARFLTPHYRVIIPDHIGFG
                     DSSRPQNEDYGSLAQAVRIRTLAQALGIKDVHVGGSSMGGHISMMYAFLYPDEVKSLW
                     LIDPGGIWSAPPGELHEIIAKTGKNPLMAKNEDEFAEIFSFVMTDHPFIPRPILNVMA
                     QERIRNYELEQRIFKQLSSDASERYVKEIKTPTLIVWGEKDRVISPQTAPILNKLLQN
                     SEIIIMKGLGHLPMIEQPKIAADDYLKFRKRLAKQGS"
     gene            complement(48615..49946)
                     /locus_tag="PLF77_00945"
     CDS             complement(48615..49946)
                     /locus_tag="PLF77_00945"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPC07449.1"
                     /translation="MSIWNKKNTIVVTCPKGVSPFLKEEVQALNYPVRAEWDTAIQTE
                     GTLQDTMALNLHLRTAQRVLYQLDSFSADTPEWLYKKIHALPWENLLHDSGPAAYLCV
                     TSIAENPLIKDSQFVNVKVKDAIVDRLRDISGNRPDSGPEKNRAVVHVYWKDNRATVY
                     LDTSGERLALRGYRKVPLVAPMQETLAAAVIMAAGWKGESNFINPMCGSGTLAIEAAS
                     IALHRAPGLLRNHFGFMFIKGFPEDHWQTLRSKARTNALKKLPARIIATDINREAISA
                     ARQNAQTAGVDHFIEFNICPFEKTSVPAGDGIIIFNPPYGERMSSDPRKPVSADFRHS
                     RETPGPGRKIILRKSGDKSGDGNRHRGREMKKLEALYKGIGDFFKKTCTGCHGYIFTG
                     NLELIKKVGLRTKRRVILYNGDIECRLLEYALYTGSLKNTAKEKSLSAENG"
     gene            50125..51369
                     /locus_tag="PLF77_00950"
     CDS             50125..51369
                     /locus_tag="PLF77_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC07450.1"
                     /translation="MKNDRKIFWGWYIVGGAFLVMAMNYGARYCFGVFLKPMANEFAL
                     SRSVISLAAAINMMVYSFGSVFVGRMLDRVAPRWITTVGAGVAACGYLLTGFVDTPIG
                     LYISYGVMVGLGSAGMGVVACSSSVSKWFISKRGVAVGIASMGISLGTVMLTPLAGYI
                     ATVFSWRSGLMALSFIMLIVGVVVAQTLMRRTHPEAYGLLPDGNTISGMPQAAEATAV
                     CPVSTMILFRDSRFWTLAVCQGLAVMISMAVFVHQVAYATDNGISKMAAASSLAAVSL
                     TGFMGQFFFGWITDRINDPKYASFLGISFMLAGTVILLNVESLLHLYVAALVYGFGYG
                     SLAPMLPLIIADRFGRQCMGSIYGLLTFFIGTGGAIGPVLGGFIYDHFGSYHNLWLMN
                     IVVLSLIAMAFLTLKKGRPYAA"
     gene            complement(51387..52550)
                     /locus_tag="PLF77_00955"
     CDS             complement(51387..52550)
                     /locus_tag="PLF77_00955"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPC07451.1"
                     /translation="MQNFVFENPTKIIFGKGQIPRIGKETARFGRRALLVFGMGTILK
                     NGVYDQVTNALEKAGVEIVDFPGVKSNPVLSHVQKGIALARGENVDVILAVGGGSVID
                     AAKAIAAGVVAHHDVWDFFTFSKPVTGALPVLTVATVSASASEMNAGAVVTREDGCQK
                     YCIVSPFIQPKVSVLDPTVLFSLSPAYSAYSAVDIITHMLEGYFNNTSSASPLQDRLV
                     ESLIKTVMESTEIILKEPDNYDARANMMWSAGLAFNGLTTAGMGQVRMPAHMIEHSLS
                     AIYDIAHGAGLSIILPAWMSYALDKKTDRLEKLAREIFHVKGRGAISAAKKGIACLKH
                     WFESIGSPVSLQAVDIPESDIDAIAGNAFALSQVWCYEGYTKDVIRKILLLAR"
     gene            complement(52618..53847)
                     /locus_tag="PLF77_00960"
     CDS             complement(52618..53847)
                     /locus_tag="PLF77_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07452.1"
                     /translation="MNGSNFQIEQQCPQCGAPIILDETDRILSCHFCRTRVYLATDDH
                     FLFYIPPAAGISEKIYFIPYWRLKGLSYAITTHNIAYKYFDANFISFDLPFFPHSLGL
                     RPQAMKLRFVGNADHEGKFIAASQKTRGKLLKNFKTQAGAVQEIFIGETASIIYTPVY
                     FANGRLYDAVLKKPLDIQKKEPEMEDKLVSSPDEKWHVDFLPLLCPECGADLPGAKDA
                     LIVFCENCHRAWNPSKKHFVPIPYATWREKGDEITYLPFWQLKVKVDGIALGNYADFI
                     KVANLPKAPLDIWDKTPFYFFAPAFKLNPALFLRWCRQLTVTPAPDDLTPDCPVKNIH
                     QVTLPVSEAFESCLVTLGSLMADKRHLADMLSSLKLTLEHYSLVLHPFAVRNRELVHT
                     KLDFSMDISALTMGAFL"
     gene            complement(53834..54952)
                     /locus_tag="PLF77_00965"
     CDS             complement(53834..54952)
                     /locus_tag="PLF77_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HPC07453.1"
                     /translation="MGTNNIIFLEVIEWFDESGKEIVHRIPEKGSGEIKFGAQLTVRE
                     SQSAVFFYKGKAYDAFGPGRHTLKTANIPLLTKVIAIPWGMTSPLRAEVYFINMKVFP
                     NLKWGTRDPVAFRDRELGLIRLRAFGVFNIRVIQPLLLINSLVGTQGIFSTEEIEEYL
                     NRVIVSRFNDYMGEKVDTIFNLPGKYNEFSEGLKVMLQKDLSKFGLALTDLYINSITP
                     PPEVQKAIDDKSRLGVFDDLNKLLKMKAAMAVEAAAQNQSEAGAGLGMGMGFMMPGML
                     LEAFKGAGTTVAADVFTCPDCRQVVPKEAHFCPACGHQILIVNKCAHCGRNLPVNANF
                     CVSCGKPVTVNPPGKQCAKCGFENLPDSMYCNKCGERL"
     gene            55099..56526
                     /locus_tag="PLF77_00970"
     CDS             55099..56526
                     /locus_tag="PLF77_00970"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPC07454.1"
                     /translation="MKKTSAEQNIYDGLALIADPIHQYILFTVPKDKSAKSAGEITEQ
                     HIIDSPWMQRLRRIYQLQSARWVYPAAEHSRFQHSLGTMHMAGEFAKSIYPSLSSVCK
                     DVPSCHYVEELLRLAGLLHDVGHGPFGHFFDDNFLSNWSLTHEIVGQNIIVDKLGDDL
                     ARVRRSPSGGFSRTERMDAQKVAYLIRMPEDGEEGRPPRWLQMLRQLFSGVYTADNLD
                     YVQRDAYMTGFSLDMVDIPRLRYYTFFTREGLTLHQSGISALRRFLNARLNLYTNVYF
                     HRTTRGLDLHLQEFFHDTMQMILPANPLNNLDDYLQCDEWTLFNHVQNWLRSKDEQKK
                     KLAAEWKKLHDREVKWKMSFVAEISLDQIQPGVGFSRAADYEQQIRERLPKPLKSLPF
                     KVDLATQDPRPLNPITEREKRINIFNPVTHKTSPEPLLEIYRFIPARVMQFRVFALNH
                     DHDGDLAAAAEKVLGSSAKSTLTNI"
     gene            56528..58588
                     /locus_tag="PLF77_00975"
     CDS             56528..58588
                     /locus_tag="PLF77_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HPC07455.1"
                     /translation="MSKNDETTDLTLFVRTTGEEIVLWDRQRIVDALVREADIDDDVA
                     GQISKEVEKQIIVSGIGLLTTALIRELVNVRLIERGLEKERRLHGRLGFPLYDVRQLI
                     LHPNKQDANIPHSPEGTNLIFAEGIKRELSLFDVFSADVGEAHATGDIHIHALGYIDR
                     PYSCCQSLEYLKINGLNLPHAINSANPAKYAEVLLAHMVRFSAVLQGHFAGTVGWDAV
                     NISFAPYLASMTDKEVKQFAQMLIYEFSQLAATRGGQALYTDIHIYYDVPPHWADLPA
                     VGAEGKPTGKTYGDYTWDAQRFAMAILEIFAEGDARGKPFILPRPLLHITDHVWKNDK
                     AREFLLRACDVAALMGNPCFVFDRDEKAVDLIFNDEERRKPWLQRRAAIQTVTLNLPR
                     LGYKTGGDEKEVFSLLEAFMEKAVKAHVQKKDFLEKLLSYAEEGPLAVLAMNHDGFPF
                     LRMHLASYVIGIVGLNELVQIVTGNEMHQKQEALEFGLRVVDFIRQEALRLSRQCKMK
                     FVLGQSLAETTAYRFARLDLRYFSPTAGRYVKGDIAEGAIYYTNSTHLNVAADVGPLQ
                     KVMREGLFHHYLEDEVITHLQLGDLNPGRNVLARFIRDVFYHSANRQIDFNPEFTFCL
                     SCKETTRGLQDKCAACGSADVEGIARLTKYFSKTSGWNKGKLAELKDRTMNDNFNNVD
                     QPKC"
     gene            complement(58585..59508)
                     /locus_tag="PLF77_00980"
     CDS             complement(58585..59508)
                     /locus_tag="PLF77_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPC07456.1"
                     /translation="MKKILLKIINPVVLFNAWLYHKKNSTFSNSTSDLELHLYSKIFK
                     NDMLHYGYFENTEIEVDTISIAEIEKAQVRYAQNIIDQIINPQDLILDIGCGMGGLSN
                     LISQKGFNVEALTPNKNQIRHIQSKYPTITCYQKRFQEFETDKKYGTIINSESLQYIP
                     LEKAFEKAESIVKPDGTWIIADYFKFDNSGVNKSSHLLDDFVRKSKEHGWEITYQQDM
                     TPHVLPTLKFVYNYAERFLLPLVHFTSGKIKRKKAWLYYLIRDLKQSVDHKIWKELAA
                     VDPIKFRNEKKYMLFVLRKNCRDAADNPASS"
BASE COUNT        14878 a        14074 c        14593 g        15797 t
ORIGIN      
        1 aacgcagcga gagatccgcc gtggtcatca tcgggcggcg cttcttggac gggtcatgag
       61 cgtctgcgac catgtcttcg tcttcgacgt tcttggccag ccactgattg gcgccggccg
      121 ggctcttgac cagttcccac tcgtatttga acagcacttt cagatagccc atatcccagg
      181 tcgtcggatt cggtttccat gccccttcaa gaccgctgga gatcgtatcg ccgcccttgc
      241 cgcttttata actgcttccc cagccaagac cctgcgcttc aaggggagcc gcttccggtt
      301 ctggtcccac atgggtcgca ggaccggcgc cgtggcactt gccgaaggta tggccgccgg
      361 caaccagcgc gacggtttct tcatcgttca tagccatgcg cgcaaaggtt tcgcgcacgt
      421 cacggccgga ggcgatcgga tcgggattgc cgtccgggcc ttcggggttc acatagatca
      481 gccccatctg cacggccgcc agaggatttt caagatcgcg ctcgcccgaa tatcggctct
      541 tgggcttgtc gctggtcgcc agccattcct cttcgctgcc ccagtagatg tcttcttcag
      601 tttcccagac gtccacgcgt ccgccggcaa agccgaaggt tttaaatccc atcgattcca
      661 gggcgcagtt gccggccaga atcatcagat cggcccagga gatttttttg ccgtatttct
      721 gcttgatcgg ccacagaagc cttcgcgcct tgtcgaggtt cacgttatcg ggccaggaat
      781 ttagcggggc cagtcgctga ttgcctgttc ccgcgccgcc gcggccgtca cccatgcggt
      841 aggtgcctgc actatgccag gccatccgga tgaaaagtcc gccgtaatgg ccccagtcgg
      901 ccggccacca gtcctgcgaa tcggtcatca gcgtgatgag atctttcttc acggccttta
      961 ggtctagttt cttgaattct ttggcgtagt caaatgcttc gcccatgggg ttgctcttct
     1021 tgttctgctg atgcaggatc ttgaggttca attgattcgg ccaccagtcc cggttcgatt
     1081 tgccgccgcc ggaaatgggt tttttcatca tgcctgttac cgggcatttg ctttcttcgg
     1141 tcatctgatt tcctcctttc tttcagtgat aggggttaaa aaaccttatt ccttttgcct
     1201 tgttgttttc tcagtaatct ggaatgattc tgaattagaa taaaaaccgg tcatcgtcat
     1261 tcttttaccg tttgtttaaa ataccgcggg actgcgcaat aagaaaattc agtctgagcg
     1321 cttcaggcaa tccctgcaat acccgtgaaa ctccagtcgt gtttcggtta cgacaaaacc
     1381 cgatttccgg gcaatgtccc tgggttcaag cgaggcggga atggcgtagt caaaaatctt
     1441 cccgcagcgg ttgcagatga gattcacatg atccgaaagg tccacatcat agcggttttc
     1501 catccggtcg aattccaatt gtcggatcag accgttttca gaaaactcct taagcgtcgc
     1561 ataaacggtg gacaaactga tacgacgcgc tttcttactc gctgcctcat aaatcaaagt
     1621 agcgccgggg tgagcttcgc tatgctccac cagcgcatca ataattgcca gacgctgcgg
     1681 ggttatcttg agaccttttt cctttaattg gccgatcagg ccgtctttat atatcataag
     1741 tattctggaa tcattccggt ttatggaaag atacaatcat gcctataaat tgtcaagaca
     1801 tattttttca tggttgaccg gcgggattga caaacgaact tatcaattat atgaaacaaa
     1861 taataattat tgagagattt catatgccgg tttatttaca agtaatcctg ctggttgcca
     1921 tctttatcgc cactgtgatc atttttcttt acattggcgg cctggctgtg cggcgggcca
     1981 gttttcagat tattgccgaa atggaagaag ccagagcctt taaagaggga agggcgatta
     2041 agattcagga cgagcggaag aacttcttcc gcgtggggac caaaaatatc cgcccccggg
     2101 cgctgaatct gctcatcgct gatggtctgg tgattaaaac caacaacggg aaatattatc
     2161 tggacaaaga aaaactcgcc caagccaaag ctcaattaag ttcaaaaaca taaaatgaac
     2221 atttcttctt aagcttatct tttatacttc cgttgttcat actgcttaat gggctcttcg
     2281 agaggtggaa aaatctcgtg aaatcccttg tgaagatttg tagcaatctt cgtgatgaaa
     2341 ttggcagtac ggtgggcggt ttctttagtt tcacgtaatg cctgttttaa gcgcacgcta
     2401 tcgccactgt cagtaatgta cttttcgtca gcttcaccac ccttgtgctc ataaacgtgc
     2461 ctacgatgaa acattagagt accaaaagat acatcctcgc tatcgatact actgagaacg
     2521 tctatgtcaa atactttgcg aaacatctcg gaaactgtcc cgatgttgtg gaagtgggat
     2581 ttctctatcc aagctttacg ggctggagtt aatggcactc tggttaggag ctgctttgcg
     2641 tagtggccgg cgaacgagtc gaaaatagcg acggtttctt taacacacgt ttcatatgga
     2701 ccaccagcat tgattctatc tctgatttgc tgaatatttt tttccaactc ctgcaaatca
     2761 tttcttgttc cacatgagct acagtaagca tacgttccaa ggacgtcatt aactttacca
     2821 caggcattgc atgtaaatag gttttgctga cgttcttctg catagtagaa agctggcttc
     2881 tcgcagtctt taccagccgc ttcagccacc gaatccatat cgattatgtg ttcgccagac
     2941 tgaccagaag caagagcgtt actaagtact tcacagtatt gcctaacata tcgttgttgg
     3001 gcatcagtaa ggaaatggta gtactcatcg gccgtaaagg cacaatacgg acagatcttg
     3061 ccaccaagac ctgctcgcca ataaccatta catgaggggc attggtggcc aaacatgcct
     3121 gtgctgtctg atgagataaa tactggaaaa cagtccggca acggaggtgg attccaagga
     3181 gtgccgattc cacccatctt aatgtcatga acggcaacgc cttgcggaat ggcataaaca
     3241 gcaaacaatg ccgcaggggt tggtcttgaa tgagtcaatc caacattgta ggcgatccgg
     3301 ccatcagcgt cgatcttgat gttaaaatta acctttcctc ctgtatgtgt aatttcctca
     3361 agttctgtct ttctattcaa tatttccccc cacggtttgg tgtaaatgtg atcaaacttt
     3421 ctcactccgt ctttgtcggc acacaacaat taatatgcgt acttcattta ttttgatatt
     3481 gtggatgcta aaaaataaca caacttttta tttttcaaaa gaaaaaatcc tatgttttag
     3541 ggctgttcaa aaatgctcag atgcaaggcg tgcaagaatc gtatcgcgag gcgtatatga
     3601 acatacgttg agcggtgcag tttgcagcac aacgccgcag atggacgttt tttagcggcc
     3661 ctggagttcg tgttttttca tcataatcaa cactgccgaa atcgcgcctt cggtcatgcc
     3721 cggaatgcgc tccatctggc caagcgtctg cggggcgatt ttggtgagct ttaattttaa
     3781 ttcgttggaa aggccgggca cggagttgta gtcaaagtca ggcgggattt tctttttttc
     3841 ctgatctttt aatttcttaa cggcgtcgaa ctgccgcttg atgtagcctt cgtatttggt
     3901 ttcgatttca atctgctgtt tgacaaaagc atccagttcc ggttcccagc cgggaaaaat
     3961 ggaaaggttg tcgtatgaca attcatgtcg tttcaaaagg ccgtggagcg ttgtgtgatt
     4021 gctcaacggc ggagaattga gtgttgcgag tttttcgttt gtttccgcag tcggtttgac
     4081 cggcgatgcc ttgagctgct caataccatt ctctattttt tttattttat cctgaagttc
     4141 cagataatgc gttttgtcaa ttaaccccag ttcgcaaccc ttgcccatca ggcgaatggt
     4201 tgcattgtct tcgcgtaaaa tcaggcggta ttcggcgcgt gaggtaaaca tgcggtaagg
     4261 ttcatccacg ccgcgggtaa caagatcatc gatcatcacc gccatgtagg cctcggaacg
     4321 atccaataga aaagcagggg ctccctgcac ggcgcaggca gcgttgattc ccgcccagag
     4381 accctgtgcc gcggcctctt cataacccga tgtgccgttg atctgaccgg ccagatacag
     4441 gccatcaact agttttgttt ccagcgtggg tttgagctgt gtcggctgaa caaaatcgta
     4501 ttcgatggcg taggccgggc gcatgatttg cgcctgttcc agtcctttga cgctgcgcac
     4561 aatcttatac tgcaattcca gtggcagaca gttgcccaga cctttggcgt aaacttcctg
     4621 cgtgtcgagt ccttcatgtt ccaacaccac cggatgactc tggcgttcgg caaagcgcat
     4681 gaccttgtcc tccagcgatg ggcagtagcg ggcgctcacg cctttgatga tgccggagta
     4741 taaaggagaa tgctgaatgt tttcgcgtat cacgcgatgc gtttcagcgg atgtggcgga
     4801 aaaatacgac ggtagccgct cggtgcgcag tttttcggtt gtgaaggaaa agggccgggg
     4861 atcgggatcg ctgtcctgcc gttccaagcg ggaaaaatca atggaagagg cgcgaagcct
     4921 gggcggcgtt cccgttttca tgcggccgat atcgaagcca agctctttaa gatggttggc
     4981 caatccgatg gacgccagtt caccggcgcg tcccgatgga gtttgcgaag ggccgacgtg
     5041 caccagcccg tttaagaagg tgccggttgt aataataatt tttttagctc cgtaaaaatg
     5101 ctcgctctga tccagcacgc ctatgacttt tccgtcttca atcaccagtt tttcaatcat
     5161 ggcctgccgg acataaaggt taggttggtt ttcgaccacg gccttcatcg tcagacggta
     5221 gagttgcttg tcgcactgca tgcgggagga atgcaccgcc ggacctttgg aggcattgag
     5281 caggcgaaaa tgcacggcgg ttttatcggt gatcttgccc atctcgccgc cgagggcgtc
     5341 aatttccttg accagttgac ctttggccag tcccccgatg gccggattgc aggacatcaa
     5401 cgcgatggaa tccagattga tgttgaacag aatcgtccgg caacccatgc gcgcgcaggc
     5461 cagcgccgct tcgcagccgg catgtccgcc gccgacaatc gcaacatcat aattgtctgt
     5521 taagttcgtc attctttcaa tcaaacactt gctcttgttt taaatcaata gtaaacacga
     5581 acaggtcatg ccgttgcaaa acggtatcca gtattttcat taagttctgg ataccggcct
     5641 tcgccggtat gacgccattg atggtacttt ctattcatga caagtttttt acggcataga
     5701 atgtaaaaaa acaacataaa aaagaggttg ctttgcccaa accggatttt tggaagaaca
     5761 ataaaaatta ctacgatctt aaaagttact ggcgcaatct gttcggctgc agcgtgcaca
     5821 agctgcagat tgatgcgggg tttacctgtc ccaaccgcga cggtcatgtg gctctgggcg
     5881 gctgtatcta ttgtgacggc agaggttcaa agttgcgtca ggctggtgag ttgccgtcgg
     5941 ttaccgagca gattgcgagc ggaaaaaaat attatacaaa acaggccgga aagttcatcg
     6001 cttattttca gacatttacg aatacttatg ctccggtgga gaaactgcgc gcgctctatg
     6061 atgaagcgct caatcaggaa gacgttatcg ggctttccat cggcaccagg ccggattgta
     6121 tcggccctca tgttgtggag ttgctaagcg gatacgcgca aaagtatcat gtctgggtcg
     6181 agctggggct gcaatctgtt catgataaaa cattaacgtt gatcaaccgc ggtcacagtt
     6241 ttcagcaatt tgaggatgct gtaaatgctc tggccggaca cggcctgaat atttgtgtgc
     6301 acatcatcat cggtctgccg ggcgaaagcg acgaagatgt gctggccacg gccaaaacgc
     6361 ttgcagccat gccgattcat ggcatcaaaa tccattccct gctggccctg gacggaacgg
     6421 ctcttggtga aatgtacaag aaaggacgca tcaacatgat caccaaagaa aaatatgttt
     6481 ctctggtggc cgatgttctg gaggtgctgc cgccgcaaac cgtgattcag cgcctcaccg
     6541 cggatggtta ccgcgatatt ttccttgcac ccgactgggc aatgaacaaa ctggacgtgt
     6601 taaactcaat cgataaagaa ctgatacggc gtgattctta tcaggggaaa aagtaccgga
     6661 caaatcgcgc tgaatcactt gattgatatt tccttagttc gaaccgcttc aacccttcaa
     6721 taagcgctat ggcttatctt tttttgaccg tttggaattc agataaaatc cataaccgat
     6781 aaaaatcaaa ataaatacta acgacggggg gaagaaggcg acaatcaaga tgcagacgaa
     6841 agcgatgaca agcattcctg aatacgggtg atcttccaat gcatccaaca tcggattaaa
     6901 caatcgatct atcattcgct ctttttcttc cattgttccg aaatcctttt gtttatgcat
     6961 ggttgtttgt gcaatgtctt attgacatca tagcataatt cttgatcttt taaagaggca
     7021 aatattccaa aacgacagga aggagtgtcc gttggtgagg cttgaaatga aaataaaagt
     7081 ccatgccgct aatttatcaa acggaacaaa ctgatgatca atgaaaatat cagtaaaatt
     7141 aagtatagaa tggtaaatat ctcggcggcc ctgaagtctt tgattttctt cgccattaaa
     7201 tcatttaact ttccgtcctt gtagatccac cagagaaatg tcacagaaaa aattattata
     7261 ataatccaca aaattgcatt gttcatttca ttattcttcc atgtacggtt ttacatgagc
     7321 atcccattcg gcttcagatt tgcacgtctt ttctaacaca ttacacgtaa tcgttttgcc
     7381 gtttttttgg gaacggaccg ccccgtatat tttatttccc ttcatctctc tcaaaacctt
     7441 taagggtttt tttgtatagt ttaataaaat gaaacattta tccagtctcg tgttgtaatg
     7501 gctctgatgg gtaaatggat ctctgaaaaa ttttttttcg tcaaccctga cccatgcgtc
     7561 acaatctttt tcgcatttca tctttaatgc atggagttgc tcctgtcgct cggacgttac
     7621 gcaaatgtca tggtatattt cttccggctt gcttccgggg atgatgcccg tccattcgct
     7681 attattatat gtttcttcat ataagacgct ttcattgtcg tcataattta ccagcttttc
     7741 tattttactc tgtttgtttc tgcaatccat ttttaacaaa atcgtctggt gatcgagttt
     7801 ctggtatttg attgatttct tgggatccga ttctctgaaa tgttcaatca aatattttct
     7861 ttcatcgtcg gcaatggttc tgtaagtcca tacagatatg ctgtcggccg attgaattaa
     7921 atttatttta ttatagtaaa aatcatcgct taaatgtttc cagactttcg ggtcgggctg
     7981 ctccgaaaaa caaacgggag gtgatgacac aatggacgct gcgatgaaaa caacgagaga
     8041 tgataaaaac atttttttca tcataggaat ctccgaacaa ttcatagccg acttatttca
     8101 taacaagttg aaagtatcat aacactgttc cttaatttaa aaaaagagaa atatcaaccg
     8161 cgttaactgg atctggaaat cttgcacggt ggctgatagg aacgattttg gaatatcggg
     8221 tgataaaaaa caagagagag aggatgaatg accggaaaac agcgcggcag cggtattatg
     8281 ggtcttctgt atacatggct tcgatgatat ctttgtattg cgcagagatg gattttcgtt
     8341 ttactttcat ggtcggagta acttcaccgt cttccgtgta gagtttcttg tcgagcaaat
     8401 ggaatttgcg gatgttttcc acgcgcgcga tttgcctgtt ggccttttcc acctcgtcgg
     8461 aaataagtct gatcacttcc tccgcacggg ttaggctcgc aaacgttgtg tattggatct
     8521 tatggtcctg tgcgtatttg acgacgtttt cttcatcaat gacaattaaa gcggtgatgt
     8581 acttgcgccg atcgccgata accaccgcgt ctgtaatata gggcgagaat ttcaacagat
     8641 tttcgatata ctgcggcgcg atatttttgc cgcctgcggt gataatcaaa tctttcttac
     8701 gatcggtgat tttgagataa ccttccgcat cgatttcgcc gacgtcgccg gtatggaacc
     8761 atccgccttc cagcgcttcc gccgtggctt ccggttcctt gaagtatccc tgaaaaattc
     8821 caccgtgttt gatcaggatt tcaccgtctt cggcaatttt cacttcggaa tccggcagtg
     8881 cgcgacccac ggttcccact ttgaaatgtt cctcgtcgga catgtgcgta atgccggtcg
     8941 tttccgtcag cccgtaacct tcacggatcg gaatgccgat gctctggaag aatttcagta
     9001 cctcatggga gatgggggcc gctccggaaa atcccagccg caccttgtca aatcccaggc
     9061 gttgtttaag tttccagaaa acgctgaaat acgccaattg ataaccaagg aataaatacc
     9121 aggggatcgg tttcccggcc agtttgaaac cgttgtatgt tgttccgatc ttaaaggcca
     9181 gattaaaaac agtgcgtttt aaccacgtgg catcggccat tttcaggaca ataccggaat
     9241 gatacttttc ccagatgcgc gggaccgcat gaaagagggt cggagaaaca tcgcgcaggt
     9301 cgttcatgac cgtgtcggtg ttttcggtaa aattgacgat atgccccgtt gtgagatgca
     9361 tcatcaagtc gaatatctgt tcatagacgt gattgagcgg cagaaaggaa atggtttcat
     9421 catcgacgct cagtttggta attttctggc tttgacgcgc aatccataaa taattttgat
     9481 gggagatttg cgcgcccttg ggcggaccgg ttgtccccga tgtataaatg atggaagcgg
     9541 tatcttcgct ctctcgtccg gcaacaatct tttcatacag acccggtctt tccttatcga
     9601 tcttttcccc aagctccaga agcttttcaa aactcatcag catgggatcg tcaaaatgct
     9661 tgaggccttc catatcccag acgattattt ttttcaggcg cggtgttctt tcgcgaaatg
     9721 ccagagcctt atcgaattgt tcttcatctt cgcagaaaaa aaccaccgat tcggaatgcc
     9781 cgacaacata ttcgcattcc gccgtaggat tggtcgtgta cacgccgaca aagataccgc
     9841 ccagcgacat gacccccatg gccgaaaaaa gccactcggg tttattctcg ccgacaatgg
     9901 ccacgtgatc gaagggcttg acgcccagcg cgtacaatcc caccgccact ttcttcacgt
     9961 tttcataata ctgtctccag ctaatgtcgt gccagatgcc gtaatccttc atgcgcatgg
    10021 caaccctgtc cggctgtttg tcgaccagcc atttcagccc ctgaggaaga gttttgatgt
    10081 cgttttcagt catatgtata cctcagcgga aacgcacttt gcggcgataa cgtttgaagc
    10141 ctttgaccga tgtttccgtg gcaatgccca gataaaattc tttgacgtcg tcgtcttctc
    10201 ttaaaatttc cggtctgtcc gcacggacaa tgcgcccgtt ttccatgaga aaagcgtaat
    10261 gcgaatattt gagtgccatg tttacgtttt gttccacaag aaagatcgtt acgcccttct
    10321 gattgatgtt ttgaatgata ctgaaaatct ctttggtcaa taagggcgaa agtcccagcg
    10381 acggttcgtc caacatgagc agtttcgggc ggctcatcag cgcccgtccg atagccagca
    10441 tctgctgttc gccgccgctc aaatgtccgg cctgctgatt tttgcgctcg cttaatatgg
    10501 gaaactgttt gagaatcccg tcaatgtcat cctggatgcc ttttctgtct ttgcgcgtat
    10561 aggcgcccat gagaatgttt tccatcacag tgagctcggg aaagacctcg cgtccttccg
    10621 gcacatagct gatacccagc cggacaattt cctccgtgtc cattcggtcg atccttttac
    10681 ccagaaattc aatagatcct ttttccggct gatcgtcatc caaaaggtgc atgatggttt
    10741 tgagggtggt ggttttgccc gctccgttac tgccgagaag cgccacaatg gagccttctt
    10801 tgatttcgaa cgaaatgccg tgcagtgccc gaatcaagtc gtaccaggtt tcgatatttt
    10861 taaccgagag cattattcct cctctcccag atacgcggca atgacttcgg ggttcttttg
    10921 cacgtcctga ggcgttccct cgatgatttt cacgccctgg ttgatcgcca gaatcctgtc
    10981 ggaaatgccc atcaccatat tcatgtcatg ctcgatcagc agaatggtga ctttgtaatc
    11041 ttcctgaata tccttgatcc agatgcgcag atcgtctttt tcttccgtgt tcatgccggc
    11101 ggacggttcg tccagcagca gaactttcgg ttccaacgcc agcgctcgtc cgagttccac
    11161 gagtttgcgt ttgccgtagg gaaggctgga aacaaaaaga tctcgcaggg actggagatc
    11221 aagaaagtcg ataatgtgtt ccacgatttc ccggtgtttg agctcttctt tcgcggcgaa
    11281 agaacgtttc ccccacatga aagcgccact cgtgattcct gtcttcatat ggatgtggcg
    11341 tcccaacatc aggttatcca tgacagtggc gttaccgaaa agctgtatgt tctggaacgt
    11401 gcgcgccaca cctttgcggg caatggtgtc cggacgcagg ccgacaaggt tttcattatc
    11461 cagaaaaact tccccggcat gaggtttgta aatgccgctg atgatattga aaatcgttgt
    11521 ttttccgctt ccgttgggac cgatgatcga aaatatctta ttgtactcaa cctcaaagct
    11581 gacatcgctg acagcccgga tgccgccgaa ggagatgttc aggttttcca ctttaaaatg
    11641 cgccatacac tgcgagcctg ctgatcgtta ttggcaacga aaatatttgg tgggaggcag
    11701 ggcatccccc gcctccggcg atattggtat gttacttttt cttccacgtt gccagttcgt
    11761 tagatgtcca gtcctgaaga ataatatagc tggaattcgg accgcatttc agaatctgaa
    11821 tggaatcgca accctgatgc actgtcgggc tccagtttac aacggcgccc aatccctgga
    11881 aattctttgt tttattgagt tctcgcagca gggcttccgt gctcagtttc gggccgaccc
    11941 gcttgagcgc atccaccaac ggttccgcaa acataatgcc ggcggtgtag aaaacgccga
    12001 aacgctcttc aggtgcgtat ttcttggcag ccgcgcgata tttattcatc agcggatgat
    12061 ccacatcggt cggcaggccg attccgccgg tgatgacgcc ttcccacaac ccaccggtaa
    12121 tcttgttcat cagcggaaaa tccatcaacg tgtcggaaga aatccattgc ggtttaaatc
    12181 cgatggttgc ggcagttttc agcgcgattg ccgcagaggt gggattgacc cacagaaaag
    12241 tcacttccgc gccggaattt ttcagtctca acatctgcga ggaaagatct ttttcgccgg
    12301 gttcaacgga tatctcttcg acaagtttca tcttatacgt attcaggcga gccttgcagc
    12361 ccttgaggcc gctcttgcca tatgcgtcgt tctgatacag gaatccgatt tttttggctt
    12421 tcagtttttc caccgtatat ttggtcagaa tgctggcctc atcatcatag agagtataca
    12481 tggcaaagag gtacggattg acgggaaaca catactcgtc aatggctgtc gacggattga
    12541 cccagatgat tttattctgc gccagataat ctttcacggc cattccgcag gcagcaccga
    12601 cgccgcccac aaaagcaaaa attccctcac gctcgacaag ttccttgacc acggctttag
    12661 tctgggcggg gttgtactga tcatcgcgga tgaaatattt gattttacgt ccgtggatgc
    12721 cgccttcatc gttgacaatt tgaaacagaa ggccgctccc tctggcgacc gatccccagg
    12781 gtgcggcagg tcccgtctgc ggcccccact gggcgatacg gatttccttg ttgtcaaagc
    12841 ccgatttggc ctgggcaaaa acaagaagcg aagcaataaa aacagcgatg acaatcgtca
    12901 atgcaatgat gaatcttttc atagtgacct ccatgcttta atgagtttag ataaatgatt
    12961 aacctcttta tgccgttatg gtctggggga tcaacccttg taccatttcc ctttttcttt
    13021 caggaacgtg aagctgtaat taaagatact gttcggacct gcgtgtccaa acgtcacttc
    13081 cacctccacc tgaaccaatt tgtccacagc gggataattg tgccggttgt ggttatcccg
    13141 cagatggtaa attctgacaa ttttacaggt gttcaatgcc gcggaagaat tttcggcgat
    13201 gtctttcaaa tattcttcgt acgtgtgtgt ctttttataa tccgatgaag gcgccaaaag
    13261 cgcatagacc tgccgcgaat cttttttcat ttcggcagtc agatagtttc gggcggtttc
    13321 ttcaatgacg cttttttcca gaaacaattc gaggcggttt cggggcgcat cccagataac
    13381 caatccgaaa acaaccagca ccgcgcaagc caggataagg attttcacgg ttttactcat
    13441 gataatgctc cgatgcttca cgttacactt tcttgatata atgtttcgcg aacagtccgc
    13501 tcggcctgac ggtaatgatc aggatgataa caacaaacgc gacgatcggt ttccattccg
    13561 gccaggcgta accgaagaaa ttttcgataa tacccaaaat caggccgccg acgacgacac
    13621 cgggaatgct ggtcagaccg cccaggacgg ccgcggcaaa agcgcgcata aacggttcca
    13681 tcataaaatt gggatccagc acggaacgcg ccgcgaagaa catggccgcg acggcaccga
    13741 tcagcgaact catcgcccag gtaatggaga atactctttc cactttgatg cccatgatct
    13801 tggctgcgtt gacgttttgc tgggtggcgc gcatggcgat gcccagcttc gaataacgga
    13861 aaaataagaa cagggatatc atgatgatga tggacaggga aaagacaccg atcgtccatt
    13921 cgttaataaa gatgccgggc agcatttcat gtgtcttggt aacggacagc agaaagggaa
    13981 aatctttctg ttccgctccc cacttccagc cggccagtcc cataagcagc atttccgcgc
    14041 cgagcgtaat aatcagaagc cccagcacgg tcggatcttt ggccggacgc agaaaacaaa
    14101 actcgattgc catgcccagc aggatggcaa aaatcagcgt aacgggaaag gcgagccaga
    14161 agggcacgtt atagaccgtc aagacatgat aggcgacaaa ggttgcaacc attcccattt
    14221 caccctgggc gaagttgatg acgtccgatg ttttgtaaat gatggcgacg gcgacgccga
    14281 aaagaccata gcaggccccg atggcgattc cctcgatcag cagcatttca aacatgacgg
    14341 actcccttca gcttttttaa aaaggccagg tcttccagta ttttttgatt cggatccaga
    14401 tgccgaacat gcccagcggt tcaaaaatga cgatcgtgat catgatgagt cccagtgcaa
    14461 tgctggagaa attggccagc ccgatcaccg taaaccattt ttttgatacc gtcgtgagaa
    14521 gtgcccccag atagggcaga ttttccacat ttttcaataa gtcgtactgg agataggtga
    14581 taagcatggc acccatgata gcgcccataa tcgatcccag gccgccgacg gtaatcatga
    14641 ccagaaagat gatggaaaga atcaggttga acgtaaaggg atcgaaaaag ccgacaacaa
    14701 aaccgtaaag gccgcccgcc acgcctgcgt aaaaagcgct tacggcaaat gccagggttt
    14761 tataaatcgt cagattgacg cccatgactt ctgctgcaat gtcgtcgtcc cggatggaca
    14821 taaacgctct gcccaccctg gtcttcatca gattgcgcgc gccgatgacc agcaaaacag
    14881 tcatcatcaa tatcagatag taaatcttta catcaccctg gatgacgtaa ctgaacgatg
    14941 tccctgaaat acccatatca agcggcgggg cggtgattcc catcctgccg ccaaagaaat
    15001 cccaccggcc gatgatgtgc ataatggtca acccgaagcc cagagtggca atcgcaagat
    15061 aggggccttc aagcctcaac gccggaaggc cgattataaa gccgaaaaag gctgcaatca
    15121 acgcggccag aatgattgca gcgaagaacg gaaagtggaa attcatcatg attaatgatg
    15181 tgccataggc gccgatggca aaaaaaccgg catgtcccag cgatatctgg ccggtgtagc
    15241 cgacaaggat gttaaggccg accgccagaa tgatgttaac catgataata tttaagaggt
    15301 agatgttata agacggtaaa taaacgggaa gggtaaaaag aaaaaccagt aatacgatgg
    15361 accacgtcag aatcgtgccg cttcggaata actcaacgtc ctcatagtaa tcgcgtttca
    15421 tgtccatggc ataaccacat atcgatggtt ggttgacatg ttgctgtacg ttgatctttt
    15481 ttgcgtctgg atgttctaaa aacatagcaa gacgtccgaa atgtgtcaag ataatttgac
    15541 caggaacaaa attgtatcag tgtgggatta atgtataaaa tgataaaaat taactgtaaa
    15601 ttgaacagaa aaattgctaa aggcatattc cagaaatttg gtaaggaatg aacaatgatc
    15661 gcgattatcg actataacgc cggaaatatt accagcgttg cacgcgctct gcagagtatc
    15721 gggcaggaat tcatcatcac ggatgatgcg aaaaagctcg atgcggcgtc gcgggtgatt
    15781 ttccccggtg tcggcgcggc gggcgaagcc atggcttatc tgcgccaaaa gaaactggat
    15841 gtctggctgc gagattacat caaaaccggc aagcctctgc tgggcatttg tctggggacg
    15901 cagattattc tggattactc cgaggaaaac gacactccgt gcgtgagtct tgttgccggt
    15961 tcgaccaggc gtttcccgga aacattagcg ttcaatggtg agttattaaa aattcctcat
    16021 atgggctgga atagcatcag gctgctgcgc agtcacccag tgtttgatgg tgtttcgccg
    16081 ccggctgaat tttattttgt gcattcctat tatcctgcac ccgccgatca agaggtggtt
    16141 ttaggcgtca ccgattacgg aatgacgttt tgttcggtgc ttgccaggaa caatcttgtg
    16201 gccatgcagt ttcacccgga aaaaagcggt cgcccgggcc ttcagatttt aaagaatttc
    16261 tgcacatgga ggctgtcatg atcagcaaga gaatcattcc ctgccttgat gttcgcgacg
    16321 gcaagctcac caaggggatc aagtttaaag gcaacgtgga tatcggcgat ccggtggaag
    16381 cggcgcggga atattacgaa cagggcgcag acgaaattgt attttacgat attacggcgt
    16441 cgtcggataa gcgtaacatc atgatcgatg tagtacggcg ggtggcggaa acgattttca
    16501 ttcctttttc tgtcggaggc ggaatccgca atctggatga tatgcggaac gtgatccatg
    16561 cgggagcgga aaaagtgagc gtgaattccg ccgcggtgga aaatccgcaa atcattatgc
    16621 agggggcaaa agctttcggc agccagtgtg ttgtgctggg catggatgtg aaacaggtgg
    16681 acgtttccga tgccattcca tcggggtatg aaatcgttgt tcacggcggc cggatttata
    16741 ccggaattga cgcgctgtgg tgggcaacgg aagcggagaa actcggcgcc ggggaaatct
    16801 gtctgaattc catcgacgcc gatggggtgc aaaccggcta cgaattgaat ttaacccggt
    16861 tgatctccga aaacgtgcat attccggtca ttgcttccgg aggcgcggga aaacccgaac
    16921 atctggcaga cgtgctgacc aaagggaagg ccgatgcggc gctgattgct tccatggtgc
    16981 attaccgcac ctataccatc gctgaaatca aacactattt gagcgaaaag caaatcaaga
    17041 caagattgtt ctggtaattt ttgctttttt tcactaggaa acagtattgc gaagtattat
    17101 aatagtaaca gttttacgct aggaggcgat ataaagtgga taaaaagagg tatgaattaa
    17161 atgtccagtt ggcgcaaatg ctcaaaggcg gcgtcattat ggatgtaacc aatgttgaac
    17221 aggcaaaaat cgcggaagan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
    17341 gcgccggatc gctgaaggcg cggccatgat ccgtacgaaa ggcgaggcgg ggacaggcaa
    17401 cgttgtggaa gctgtccgtc acatgagggc catgaaccgg gaaatcggtc aactggtgca
    17461 gatgcccgtg gaagaagtga caggattctg caaaaccaac ggtatcccgt acgaacacgc
    17521 gctcaaagtg aaggaattga gccgtttgcc ggtggtgaat tttgccgcgg gaggcattgc
    17581 cactccggcg gatgccgcgt tgatgatgca actcggctgc gatggcattt ttgtcggctc
    17641 cggtatcttc aaatccgccg atccggccag acgggcacgg gctatcgtca aggctaccgc
    17701 ttatttcaat gatcctcaaa aagttctgga agcgtccatg gatctgggag aagccatgcc
    17761 gggcctggaa atttcgcaga ttccgccgga acagattctc gccgggcgcg gctggtaaat
    17821 ctttatgtca ctgcgtttag tgagcgataa accttctgtt atcggtgtgc ttgatttaca
    17881 gggcggtgtc catgagcatc tggagcatct ggagcggttg ggtgtcgcct gtaaaagagt
    17941 aaagcagacg gaagatttta aagacctggc gggcctgatt attcccggcg gggaaagttc
    18001 ctgtctttcc cggttgctga ctattttcgg catgaaggat gttttgctgg cggcgcaccg
    18061 ccggggaatg aaaatctggg gcacctgcgc cggagcgatc ttgctggccc aaaacgttgt
    18121 cggcgaagac gcctgcctca aactgatcga catcaccgtc gaacgcaata gtttcggttc
    18181 tcaactggac agttttgccg gcgaagcgtt gattccgggt atttctgccg atcctgttcc
    18241 gctgacgttc atccgcgcgc ccaaaatact tgctgctcaa cccgatgtcc gcgtactttt
    18301 gaaaattgac gactacatag cagcagcgga aaatgactcc gttctggtga cggtctttca
    18361 tccggaatta accggctgtc tggccctgca tcagtacttt gcggttaaat gcggtctgaa
    18421 aaccgcaacc gggcaagccg gacattcgga tccggactgg gatatttcaa gctggacaaa
    18481 atatgccgcc cttgccggca gagccggtga ctgacgaaaa ctgtcgttta aaaaaattgc
    18541 ataacaatcc caaaatgctt ggtctgcaca ttgaaacaaa atggtgttga caggtcaaaa
    18601 tatctttttt aaagtgcgga catattaagg agcgtcgtgc agaatatttt ttccgaactg
    18661 aaaacagatt cccgcttcat ccgttttgtg atgaaggtgg gcagtccgtc acccctggat
    18721 cccgacgaac aacacagacg gataccggcg gtgtttgtgc tgatgatcat cagcgtcgcc
    18781 acggcggttt ccggcatcta tcattgtagc agcggaaaaa atccccttgt ggttttggac
    18841 gtcatcggtt ttatcctttc ccttatcatg ctgctttatc tccgtcaggg aaaaaatccc
    18901 ggcatcgcgt acggcatgat cagcacgggt tttgttctct tgtgttgtgc ggcgactgtt
    18961 ctgggcagaa cggagatgtc gctgatcttc tgggccctcg tcctgcccgc cggatgtttt
    19021 tccatattgg gccggaaaat gggtatgttc gttgccgcat tctttttctt tatcaatctc
    19081 ctgctgatga atttaccgga gcatatgatc ctatcaaaac cctttccggc atcagtggct
    19141 gcccgtttca gtgttgttta cctgatcctg acgtttatgc tttattatta cgagtcatcg
    19201 catcagatgc tgattcgcta catccggcat gaggagaata agtttcaaaa tgcttcccga
    19261 tacgatccgc taaccgggct ttccaaccgc agggatattc ttgaaaaaat ggaaaatgag
    19321 cgggagcgtc attcgcgtct ggaaaaaact tttacgctca ttatgggtga tatagataat
    19381 tttaaaatgc tcaacgatac gttagggcac gaagccggtg actatgtact gcaaacgatc
    19441 gggcgcatcc tgaagaatca ggttcgtgga atcgattgtt cttcgcggtg gggagacgat
    19501 gagtttctga ttctgctggt ggataccgat ctggaaggcg gacagcgcgt tgccgaaaga
    19561 atcagaaaaa aggttgagaa taccagcttt cagtatcagg gcaagggact tgccgtgacg
    19621 atgacattcg ggttgagtat ttatcagagc gccgacgaac agatcgacgc atgcgtcagg
    19681 cgttccgatg acgcccttga ggaaggcaag cggcagggga aaaacagagt ggttcccgtt
    19741 tagtgtgttt catatctgtc aaggaagagg ttcaaattgt tttcttcgtc gactccaagc
    19801 cggttataaa tgaaagattt tcagaaaaat ggtcgccagt cccaggaaag tcaaaaaacc
    19861 gaaggcgtct gtaaaagctg tcaggaaaat gtgcgatccc agggccggat ccagcttgag
    19921 gcgctttaac gccaggggga ttaacgcgcc cacaagggtc cctgtgaaaa tactgataat
    19981 catggccaga ccgagaatca gtcccagaat gggtatgcct ttccagaaat atgcaatgat
    20041 gcctatgaca attcctacgg cgatgccgtt ggcaatgccg acggcaattt gtctgaacaa
    20101 ggcgcgcttc gcgttctcaa aggtaatttc tccaagcgcc atcccgcgta cgataagcgt
    20161 gagggtctga ctgcccgcgt tgccgcccaa tccggctaca accggcataa agaacaccag
    20221 cgccaccatc aatttgatcg tttcttcaaa aaatccgatg accagcacgg aggcaagagc
    20281 cgttataaga ttcaaataaa gccaggggag tctttttttt actgattcgg tggtgttatt
    20341 gaaaacggtg tcgtcttcac ccaatccggc gatgcggtaa atatcttccg atgtttcttc
    20401 ctggatggca tcgacaacgt cgtcaatggt aatccgaccc agaaggcgct gttgattatc
    20461 cacaacaccg atagagacca gatcgtattt tttgaaaaga cgggccactt cttcctgatc
    20521 catgtcgtgg agaacactgg gaatatcctc gtcgataata tccagaatcg gcgtaaagcg
    20581 tttcgttgta ataatacttt gcagaggaac ggttccgatc aatttatcgg tttcatcaac
    20641 gacaaaaatg ttatgaatat tttcgatttc atctttggcg tcggcaacgg cctgaaaggc
    20701 atcgttgatg gtcgcgtctt ttcttacgga aaccagttcc gactgcatga taccgccggc
    20761 ggtatcgtcg gcgtatttga gcagttcctg aacttcctgg gattcttccg gttcaatcgc
    20821 ttccagaacg gccttggcct ggtcatcgga taactccgcg atgatatccg ccttgtcgtc
    20881 cgagtccatc tcttcgataa tgtcggtgag tttttcgttg gaaagatctt cgataagctg
    20941 ctcacgggat gtatcggata attccagaat aacgtcggac gctttgtcca catcgagcag
    21001 cgagaaaaga tgtaagcggt cttcttcttc cagttcgtcg atcaaatcag cgatgtcggc
    21061 cggatgcata tcggagaaaa gatcgatgat gtcgaattcc cggttgcggc tgatcagatc
    21121 ttttaatgtt tccagcgcgg tcggttcttt tttcttgacc ttttgcatag gatcgcctga
    21181 tgttgaagtc ggtttaagaa tgattttgtt gattcagcaa taaactagag ttcttgctca
    21241 tgcgtattta cgggaaagaa gattcttttc tgaaatattc gcacgtgaag attcgttttc
    21301 cggcagcgag actataagga aattcaatac atatgtcaat gtttttctga aaaaaaagtc
    21361 ataaaaaaca atttttgtca taaggttcat ccgagccgac ggttgttttc tgtatttgaa
    21421 aatcctgcat ccgggtgaga tttttttctc gttgacaggg gaaatgactt gcttatattc
    21481 tctgcgggag ttttattttc ttggcgcaaa ggggacgata cttattaaag cggacaacat
    21541 tgaaaaaaat ttcttttctg tcggtcgcta ctggggaaac cttaatgcat cgttgaagag
    21601 aaaagaatcc gtctggtgga tgaataccgg tgtggctgtt tccgatatga attgggcatg
    21661 gaatgaaacc ccgctggcgg ccccggatac ggcaatcgtt tccgaaatca gggaagatta
    21721 caaaaaaaat aatttgcgtt tctggtggtg ggtctatcca tgcggacaat ccgcggccac
    21781 atccgcaata ctgcagaacg caggatttcg tttatatgcc aatgtctgct gtatggcggc
    21841 tgatttaaac gaatcatttc cggagccgta cgcgaccggt gacatggaaa tatatcatgt
    21901 ccgcagccgg gaggatctgg ccgtgtggga ggacgttgct tttcatggtt ttgagatgcc
    21961 gcggcacgcg agagacccat tcggggcctt tgtttcttct tttgaaacag gagatcagtt
    22021 tccacagagg tttattctgg gttgtattca agggaaacct gtggcgacgt ccctgctttt
    22081 taaggatcaa cgcacggcgg ggatatacta tgtatcaacg ctcccctctt acagaaacag
    22141 gggatgcggc ttgagcgtca cgcaggccgc gatgcaattg gcgcgggaaa gcggatttaa
    22201 gcatgttgtc ttacaggcga cgcccatggg gaaaagagtt tatgaacgat tgaatttcaa
    22261 acgatactat catgccggaa tctatacctt atagaagatc atgacgcttt tatcctcttg
    22321 acaggcatca tcgcgttacg tatagtccgc cagacaaaac cccataagct attcaaaatt
    22381 gcaacgtgct gtcggcaacc catgataata tctttctgaa aaggacagtt catgaaaaga
    22441 aactccggtc ataatatagc atgcgacaat gagtgcggtg gccttcatca atccaacccc
    22501 ttgtcccttg ccaaaaatga aatatcctcg acgctttcct ggtttatgac ggataatgcg
    22561 ctatatccgg gtgtggataa acactcttcc ccgctgcgtt atgagaactg gtcgggacga
    22621 tatagcgaat atatttccag acaagaagcc gaatccatcg tgagccgctt tatctgccct
    22681 gagaaagatg aaaattttac actgtttttc gtcagtgatt ataaggatac gtacctgggg
    22741 aaaaattttt taaaggacgg cagcgatatt tatctgaaac attaccggat gaccgatgcg
    22801 ccccgggaaa tccgtctgag aaatgttttg cgccggtgcc tcgccagaaa aaacttcaat
    22861 gctttttatc agctgcatca aaaaaacatc gaagccgtta agcctttgtt ctatgttatg
    22921 aagaggggcc gctggattcc cgatgaggtc atcgctgtca gcaagggggc gtccggcaac
    22981 aaaacaattg agaccctcat ggcggacagc cgggacaatg atgaattcag gcttctggcc
    23041 gtcaatctcg gctcgtatgt cgccgaactt caatccaggg aaattctttt taaagaattg
    23101 taccggaatc tgatcgcggt caaccagacg acgcattggg attttattct ctgcgatctg
    23161 gatgaaatca ggtcggtctc ggaaaaaaga ttttacaagc acaggaagca cgttgaaaaa
    23221 ttacgcaaga aaataaagcg ctacggcgat tcttttctcc atgcttttga tcaggggtat
    23281 cagacacgga tgcatgatca gacggccccg tcgagacggt gatttatcgg ggatgtttgc
    23341 cgaagggatg acgataaaaa ataccggcaa tgggttgtcg gttaaattct tccatacaga
    23401 tcttcaaaac gttctatatc gccatcatca cagcgtgctc ccgtttggac ctcaataatg
    23461 accagatgac cgtctgcatg gttcaggacg cggtgtttaa cctttgccgg gatatccacc
    23521 gatccgccgg cgctcagcga tatgatcttg tctccaacgg ttacgtctcc ggtgccggca
    23581 acaacaaacc agtgttcgga acgaaaatga tgaagctgta gactcagacc ccctcccggt
    23641 tctacaataa tgcgtttgac tttatggccg gcggtctccg ataagacttc aaaactgccc
    23701 caggggcatt gggtcatttt tcccattttc atcacctgta agatttgccg catatctaac
    23761 acaaattttg aacggggaaa agcgctcttt ttcattttcc atactcgctt tcgaatttaa
    23821 attatgatac ttacacatac cgcgcatcga tcatgtcggc ggaaagtaac ggggtttgat
    23881 gaacatttcc tacacggtca ggcgaagcag aaaaagacaa aaaaccatgt cgcttcaggt
    23941 ttgcgatgac gcgaaaatcg ttgtatccgc tccttctttt acacccgccg atgacatcag
    24001 acgttttgtg gaggaaaaac aaggctgggt ggctaaaata ctggcgcgac agaagcagga
    24061 ggccgctcac gccaaagaga aaaaatatga aaccggcgag caattttttt atctcggaca
    24121 gctctatccg ctggaagtct tctttgaacc ttttgagaag gaagggattg ctttccggaa
    24181 tcatcgtttt tatcttaatt gccgcagcga taaagtgtta agaaaacaat gttttgttgc
    24241 atggtattca aagaaagcgg acgagatgat tcgtcgtcgt gttgattttt tcagcgggat
    24301 gcttaaactg ccttatgaaa gaatcagaat cacatccgcc ggaagccgtt gggggtcctg
    24361 ttcggaagat aacagccttt ctttcagtta tcgcctgatc atggcgccgc cggatgtcgt
    24421 ggattacgtg attgtccatg aattggtgca catccgggaa aaaaatcatt cgtcgacatt
    24481 ctggcaaaag gtagaggcgg ttatgccgtc gttcagagaa cagcgtcgct ggctccaaga
    24541 taaccatagc aacctggcac tttgagcgga cgaaggaaag gaatgataac gtatcgacat
    24601 ggaacacatt gaaatcattg atttgaaaca ccgggatggc ctggcggagc gtttcaaaaa
    24661 aatgaatctg cggatttccg attatacatt tgccaatgtc tatctttttc gcaacattag
    24721 tcattacgag ttcgtgacca aagaatgcgg tttgttcgtt tccggctgga ataagcagaa
    24781 tcaaaattat gtcatgccgt tgaatgatcc gcgcgggtgt gacgtcaaaa catttgctga
    24841 aattcttgga gcaagaaatt ttttctttcc cattcccgag gagtggcttg tttattttcc
    24901 aaaagacaaa ttcagcgtca gttatgacga aagcgaatcc gactatctgt accttaccga
    24961 aaacatggcc gggtttaagg gaaaacaata cttgcgccac cgcaaccatt taaaacaatt
    25021 tctggcatcg tatattcccg atggcgtgac cctgtccagc cataactgca acgatgcgat
    25081 gattgttctt caccaatggc aggaggattc ccgcacggca tccgacaaaa cggacttcgt
    25141 ccaatgcagc gaagccctgc aaaatttttc cggccttgcg ctctggggaa taatatacta
    25201 cattgccggc aaaccggccg gttttatcat cggcgaagcg ctggcggggg atacgttctg
    25261 tctgcatttc gccaaagcat cgaaacggta ccacggcatt tacgaatata tgtttaatga
    25321 tacggccaaa agactggagt ctgcatacaa gtatctcaat ctggaagagg atatgggtaa
    25381 taagaatttg cgccggacca aaagttccta cggtccggag tttttgctga agaaataccg
    25441 tgtcggaatt atctaattgg aaaaaaagac agcgatgagc gaatcggtta atataacatc
    25501 actgcaggaa ggcgatcagg gagtgattca tgccatcgaa ggcgggtcgg ccattaccag
    25561 ccgctttgcc ggcatgggga tcgtctccaa cgccaggttc cgcattgcgc agatcagcgg
    25621 cggcctgatt gtgcttcagg ttgccaatac ccgtatcgcg ctgggacggg gtgaagcttc
    25681 aaaaatcaca gtttccaagg tcgctccgga ggccgatgta tgtatgccgc ccatcgaaaa
    25741 ggaaatttcc gtggcgcttg tcggccaacc caatgtcggc aagtcaaccg tattcaatat
    25801 tctgaccggt ttatcccagc atgtcggcaa ttggccggga aagacggttg aaaaaaaaga
    25861 agggacccat cgtgccgaca gcgttttgat cagaattgtt gatttgcccg ggacttacag
    25921 tctgacggca ttttccgaag aagaaagaat tacgcgtgat ttcatcatca aggagcaacc
    25981 cgggctggtt gtgctggtct tgaacgcaac ggctctggag cggggtcttt atcttctggc
    26041 cgaagtgctg ttgctcaatc ggccggtcat cgtggtcctc aatatgcttg atgttgctgc
    26101 cgatcaggat attgaaatca acatccgggc actggaagat tctctgggcg taccggttat
    26161 tcccatggtg gccaaacgca acagcggcat cagggaactt gtttctcata tctcctcgat
    26221 tgcgtccagt gaatacaaat ttcaaccggt aaagccggaa gtatccaccg atcatcagtt
    26281 gatctatcag caaatcctga cgctggtcag accctatatc catgatcctt acacacccga
    26341 atgggtggcg gtgaaattga tggaaggcga cccggaagtt acccaacttg tcgaacagca
    26401 ggttcagacc atcgcgcgcg atcaaattga aaaactgctc atcaagcatg aagacgctct
    26461 gcatgctgtg gtcaacggcc gctacgattg gattgaagaa atcacccgtt ccgtcgtttc
    26521 ccgttttaaa atgggacagg tcgtgttgac cgacagaatc gatcatgtcc tgacccgtcc
    26581 ggttttcgga attcccatcc tgctggcagt gatggccctg gttttcttcc tgacctacag
    26641 tgtgggtgtc ccgcttcagg aatggctcag cgaccgtatt cagcaattta tcggatttgc
    26701 cgaacccctc acgaccgttt ggccttcgtg gttgagcgga ttgctgctca atggcgttat
    26761 cggcggcgtc ggctcggtgc tgacgtttct gccggtgctt ctgatatttt ttgccatcat
    26821 ggcttttctg gaggatgtcg gctacatggc gcgcgctgcg tttgtgatgg acaaaatcat
    26881 gcaccttgtc ggattgcacg ggaaaagttt tattccgatg tgtctgggat tcggctgcaa
    26941 tgtgcctgct gttttgggcg cccgtatcat tgagacaaaa aaagccagaa tgatcacgct
    27001 gcttctgatt cctttcgttc cctgtaccgc gcgactggcc gtgctgacgc tggtatcagc
    27061 ggccattttc ggtcaaaacg gcgccttcgt tgcgtggagt cttgtggccc tgaacatcgc
    27121 cgtgctgggc attgccggcg tttttgtcaa taaaacgctc tggaaacagg atgcgccatt
    27181 catcatggag ctacccattt atcacacccc ggatgccaaa accattatga tggtggtttg
    27241 gacaagaaca ctttcattta tccggaaagc cggaacggtg attcttgctg tttcgctgat
    27301 tgtctggatg ctttcgtatt ttccgaatgg aaacgtcgaa gaaagtctgc tggcctcctt
    27361 cggcagactg cttcagccat tgggcattcc actgggactg gactggaaaa tgattaccgc
    27421 tcttttgacc ggtctggtgg cgaaagaaaa tgtcgtggcg actctgggcg tgctttattc
    27481 cgtgggcaga gacggtctgt ccaatatgtt gccggccatt atgagtccgg aatcggctgc
    27541 ggcgtttctt gtggtcatga tgcttttcat cccctgtgcc gccacaattg ccgttttgaa
    27601 aaaagaaatg aacaacaata aatggtttta tactgctatt gtcatgacgc tgctggtgtc
    27661 gtatgtcggt ggaatcgcgg cttataattt cgtcaaatgg ttgggtgttt aatcaatcaa
    27721 taaccgattt ctctaacgac cgggcgatag agaaacatat tgcggcggat aaagctgtcc
    27781 tgcttctcca tatcttctga tgaattacag acgtaagtca ccacaagata tttccccctg
    27841 gaaaatccga tgtgttcttt tcccgcgtaa caaaaatttc ctttatcgta cagagcgctt
    27901 cggggatcca cttccgggac gggagcgccc agaggtttcg cttcactcca cggaccttcc
    27961 agtttgtcgg aggtctgata cagcaaccgg tcccctttat tttgggtgct taaataaacg
    28021 gcgatccact tttgatcatc ttcatggtag cggacgctca attccgagac actggcgtcg
    28081 agaacgattt taactttttc cgggctcagc cggttttccc aggttccttc cttggtgtaa
    28141 tattctatcg ccccggcagg acgatcgatt ctgtcgacag gaatgcgggc cagaatattt
    28201 ccgaagacat tcaaatcgtt tttgacgtga acatagaaag ggtagaaaaa aacatagcga
    28261 tcatgcacaa cggatgtggc ggcaaaggcg gcaatttccg gaggcagcgc gtgcgtcaaa
    28321 tcgacgtaat cataattcca attgcgggga tcggtcgcgg aaaaatcttt tacccggacg
    28381 aatttgtgac cggcgacttt gaaattgaat acctcatctt ctttgtctcc cggaacaatc
    28441 gatatcagcg gaatatataa aacgcggtta accataaacg gatcctgcgg ccagagccat
    28501 tcattcttgc cgaacgatga cacatattta ccgccgctgt gttgcagata ataggtgatc
    28561 tcaaatccgc gattttccgg gcaggaggaa atcgcaacgg tggtgcccag gacgatgtcc
    28621 atatcaatgc ggtctcgtct gcatcccggg cagccgacaa acgtatctcc gaaaacccag
    28681 agtgtccgat ccttgtccag tttaatggag taggcgccgt cgccgccgta ccagccgtcc
    28741 ctgtcgggaa aaaccggcag gcaatgaccc ggcgagacgt ccgcatcgta tcttgcggca
    28801 gtaaacgtgg agcaggaaag aagaggaata acaacgaaca ggaaaataaa gccccggcag
    28861 acattccgga tcaaaatatt tttattcata acatcacatc aaatggtcaa atagaatttt
    28921 tacgccaatc acaattaaaa caattccgcc gaagatttcc actttccggc cgaataattt
    28981 cgccagcgcc ttgccaaaaa tcatgccggt agccgtcatg acgaaacaga cgacgccgat
    29041 gacgacggca gggaacaaaa tcggatgttt caagacggaa aaactgaaac ccaccgccag
    29101 agcgtcgatg ctggtcgcaa tggagagcat cagcagcggc cagccggttg tccgatccga
    29161 ttgtgtttcg tcaatgcctt ttccgcaacc ttccaggatc attttccccc cgatcagcgc
    29221 cagaagtccg aatgcaatcc agtgatcgaa ggaggcgata acctccacaa cggtcagacc
    29281 ggccatccag ccgatgatgg gcatgataaa ctgaaagaat ccgaaagcaa tcgcgagccg
    29341 caacaccggg atccatgatc ttttctgatg ggcggcgccg ataccgatcg cgacggaaaa
    29401 tgcgtccagt cccagtcctg tcgcaatcaa gaatattgtt atgaagccca tgacggtcca
    29461 tcattcattc tgaattattt tcagagcgtt aaagattttt tctgccaaaa ccttaccaat
    29521 tcccgggacg ttttgcaatt cttccttcgt tgcatctttt atttttccgg cagagccgaa
    29581 atggctcagt aaaattttgc ggcgttttgc accgatgtcg gcgatgtcat caagtacgga
    29641 atgcaaatcg cctttatgtt tgatctgatg atggtaacgt aatgcgaaac gatgggcctc
    29701 atcccgcacc agttgaagaa tgcgcaacgc ctgcggccag gcggataaat agagggcatc
    29761 tttgcgtccc ggcaggtaga cgcgatcctc ccacgtccgg gttgtctttt gccccgcgcg
    29821 gacgggacag cgccggcatt tttcttttgc caggccgatg acatccgtcc tgatcttcag
    29881 atctttcagc accgaaagcg cgacgttgag ttgccctttc ccgccgtcga caacaaccag
    29941 gtcaggcagg tgttcaccgc tggaaaaccg tcgggaaagc acttcctgca tcattttata
    30001 atcgtccggt tccgccggcg ccgtgacccg gtaacgccgg taactgtttt ggtcggggtc
    30061 accgtcccga agaaccgcca tggaaccgac ggcgtatgtg cccccgatat tggaaatatc
    30121 ataacattca atacgtcgcg gcaatccggc gagtgataat ttttcctgca gtatttgcag
    30181 ggcgaattct ttttcttccc gcttcttata agtggattcc cagaggttgc gcgcattgtc
    30241 gttggccagc tgcagcaggg cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30361 nntcctgatc cttttcttct ccccgttcaa cgttttgttt ttccagcgcg tgttccagtg
    30421 cggctatccg gtcgcgcaaa cgggcggctt cttcgtaatg gagctcctct gacgcgtcgt
    30481 tcatctgttt tttcagttcg gtaatcagct cccggcttcg tccccgtaga aacgatacgg
    30541 cgttgtcggc cagtttccgg taggcctcct cgttgattcg tccgctgcaa ggggcaagac
    30601 accggccgat ctggtattcc aggcagggcc tcgggcgaag cttaaaatcc ctgttgcggc
    30661 aggaacgcaa aggaaatatc tgctggacga aacgcagggt ttctttggcc gccagtccgg
    30721 aagggtaagg accgaaataa agcgcagcgt tatgtaatcg tttgcgcacc agttgcagac
    30781 ggggaaattt atccgtcgga tccagcgaca ggtaataata ggttttatcg tcgcgcagcg
    30841 taacgttgta acgcgggcgg tggcgcttga ttaaattgtt ctcaagaatc agagcttctt
    30901 tagccgttgc ggtgttgatg aattcgatgt cataaacgcg cgccattaga aagggcgtca
    30961 tcggacggct gtctttcccc gtgaaataag acgccacacg gctttttaaa tgatttgctt
    31021 tgccgatata gataattttc ccggccggat ccttcataag gtagacgccg gggctgcgcg
    31081 ggaaaagagc gattttatct ttcaggacgt tgttttcttt catcatttca gaaggcgacg
    31141 accattttgc accggtcgtc agccgtgtct gtcttagagt ttcggtttat tgtatcagtt
    31201 cctgaatcag aagcaaagcg gctccgccga ttcccgcggt tgtgattaaa aataccaacg
    31261 tgctgacgat accgaaaaag cttccggctt ttttattgac gttcggtttc acggcaagaa
    31321 acagaaaaag tgacggattg gccagcgccc ccgcacatgc caatacagcg acaacgagag
    31381 gcaggttaat gccggtggca aaaagcacga gcaggaggat gcccattatc acataatcaa
    31441 cgtgagcctt gataagcttt tcttcgtcgc ggatgatttt cttcatgacg gtcagagata
    31501 ggtatttgcc cgcgataatg aaccagccgg tcgttatcgc cagcagaatt aatacagccc
    31561 cggttatgat gagatatttc atgatattac ctccggaaga tattagtgtc aggtcttatt
    31621 attcatggat ggctgttttt tatgttgtcc atcagccggt cacagatagc cagcgcaatt
    31681 tttttattgg aggtatcatt gacgtggatg taatcggtga agacagcatc ccgagaattg
    31741 cagaatatgt cactgagatc atgaaattca aagggaaacc gtttttgctc ttcccggatg
    31801 ttgcggagaa ttcttttccg gacgtcgttc gtatatggac ggacaatgcc cgcttcttcc
    31861 ggcggccatc cttttaaaat atttttttcc gtatcggaaa cggcgaaacg tttatagaag
    31921 attatgggtt gcagaaaggc aacatattca acatggtagg cgcggcagat ttgtcccgct
    31981 tttgccagat tgccggcata aattccggct attttttccg accaggcata ttctccgtgt
    32041 tgtgccgaaa gtctcaggtt ggaaaaattg ccgaattttt caaaaaaata attgcggaac
    32101 acgtgccgga gcatttcgct gccgtatgcg agcagcgcgg gccaggggta atctcctgtt
    32161 gcctgaaata gaacattgtt ttcataagca aggaagttaa agggatagcc gggccgggga
    32221 tcgtaaagca gagggtccat aatatcgttg ccgccgtcgt agaacaccac catgtccggt
    32281 tccgagtcca acacgtgaaa gacaaggtga gccagttcct ggccggaatt ctgcgatatg
    32341 acgccgtagt tgaagacgcg cacgttctcg aatccgcggc gcttcagttc ctgttcgaca
    32401 tagacgggca acggcggatt gccgtattcg accgtcgaac cgccgagcat aaatattctg
    32461 aattctcccc gcgttttggc cggttcttgg ctgccgtatg cgaatcggct gaacatcgtg
    32521 tagggatttg cataacggat atcgatcctg tcgtcaaaag gggcaatttt aggataaaga
    32581 taccggaaaa acaattcggc gaccaaaaaa gaaatcagca gaaaacaaag aatgaagaga
    32641 aatcctttaa gccgtctgga catgatggct cctgccgcat aaaatcaacc aggatgtatg
    32701 gtctgttccg gaaaacatta ctgccgctgc cttttttatg tttttcccga tgttccgtat
    32761 tcggtgaatt gcgggatgta gtttttaaac attttccgta tttcaggatt tttttccgtg
    32821 tcataaagcc gttccataat gcctctcaca ccgtgcttcg accgtacaag agactcgccc
    32881 tgctccttat acagggcaac ggcggtctgg gatacagccg aacagacggc gtctatgaac
    32941 ctttgccaga aatgcaatcc ctgcccttta tttgtggggt gcaacagaga ccggaaaaaa
    33001 atgatggacg agttgaacgt tgccctcacc ggtgcgccga ctattttcga tatttcatag
    33061 accatggacg caataatcag cggactttgg tgcagcgaat cgggcggaat tttgttggtt
    33121 tcggccagat cggcaacgga aagggagaga aaataatcca tcgtgccttt tttgccgtaa
    33181 atgaacgtat tattctcctg gctttcatgg aaaaagaccg agccgggcaa gtcaacgatg
    33241 gtgctgggct gcgctccgat tcggtttcgg atacagaatt tcgtaatgtc aacgccggtg
    33301 ttgtcggttg gagaattaaa atagtcatag ggcaggccca caataaaaga tccgtgaacg
    33361 gaaatgccat gagcatgaat cttcctgatt ttggatgcgt aatatttttt taccgacatg
    33421 ccgctttttt cgatatcttt aacaatatgc ttattgatat gcttcaaatt gcgtatatcg
    33481 agggattcca gaccgatgaa gaaatgggtg cctcctgatg ctctgatcag tttcagtaat
    33541 tcttcgtcgt cggcaacttc aatggagatt tgaaccgcgt agttaatgcg cagatcgctg
    33601 gcaatgattt tttttaagaa ggatatcaga ttttttttat tggagaacag attgtccggc
    33661 gtgaagaaat agtaccggtt tttaaaatca acggtttcct tctgataaga acgaagctcc
    33721 tcaacaaagt catccgtgtc ccgaaattgg acgattttat ttctctgcgg cagagtcgat
    33781 atggagcaaa acttgcagga gaatatacat ccggtgaatg gtgcgacagg attaatccgg
    33841 aaatctttca ggggggtgta tttgatcacc ggcagtctgt tgagcagatt gattttcagc
    33901 ggttccaggg gctcaaggtt ttcaaaattt ccccagaccc cgttttcaat cagggtttct
    33961 cccgagtagt cgcttttcag ggaatgcgtt ttcaaatcat tcaggatggt cttgaggacc
    34021 gtcgaatcgc ccgcccccct gacaatggaa atcagttcgg gatagggcgc atattttctg
    34081 atgaacgtgt ccgcatcttc aggcatggtg gaaacatgta ttccgccgat gatgaccgga
    34141 atttttgcgt gattcagcac gatggccgca gcagcggcgg ctggaaaact ggagctgatg
    34201 gtggaaataa aaacagcctg gggaggggcc tttttctgtc tgatgacttc tgaaaaattc
    34261 cgtcgcttac cgtccaccat gacgacgaga tgatcctggg ccagttttgc cagctgatgc
    34321 tggatataga tgcctgcata catttcgatg gccagggcgt caaccattgt tgcccgcttt
    34381 ttttccggct gaaaatcgat gaatcttaaa taattttcag cattcatttc gtgagcgttc
    34441 tgtccggaca gataggtaat cacatcaaac ggcgtgatgg aattggccgg catcagatcc
    34501 atcaattgat aggtcagcgg attataaacg ataaaataat ttacggacat tgtttaattg
    34561 agtcctttct catattttta acaaaaacat tccagcccat gtacagaatc atggggtata
    34621 tcgtaaggac gatgcccact gaagcggaca cgtatccgga tttgctaaat cgccagcctc
    34681 aatgtctttc ttgagctttt atgatttttg ttgtgcaagg catagcacag agtggtttcc
    34741 tcaaacaagc ttaattttaa tcatcctttc ggttttttta tttgtcttgc gggaaaatgt
    34801 ttattttttg tttatccccg gtgcctgcgt tttcaaaaga tgcttgtcat gcaacgtggc
    34861 taacaggagg agcgacgcca tagaaccgat cagacataaa aacatgtcgg tttgggtatc
    34921 ccagagatat ccctgggttc ccagaaaagc ttccgtgtcg ttggggttgg agagcgatgc
    34981 ggcccattcc agaatctcat ataatgcgga aaaggccagg gggacggcaa gggagaaaaa
    35041 agccagccat tttccctttt ggaccggtga ggttctggcg ataatttcac gcgcgtatat
    35101 cgccggaccg aacccctgca tgagatgccc gattttgtcg taattattcc gcgtccagcc
    35161 gaaccattct tccatccaga ggcccagcgg tactttggcg tacgaataat gggcgccgag
    35221 catcaggatc atcgcgtgga gggcgattag cgtataggtt aaatccgtca gtctgaattt
    35281 cttgtatgtc aagagtatga tcggcaaggc catcatgaaa ggcagggttt ccagaaacca
    35341 tgtcagatag gtgtcttgcg gttttatggc cgaccagatc cagacgatca agacggttgc
    35401 cagcaagatc agatgatatg tacgtacggg catattttct tacggtcaat gtgcgtgaag
    35461 aattttactg caatctactt cattcaacgg aaaaaataaa gaacgattta tggtgcttcg
    35521 gaccatgatg attggagtcc attatttctt gattttgtga tacatcacca tacagaacat
    35581 aaaaaagttg ccggagcgta aaagacacag gatgaaaaaa attttccttc aattttttct
    35641 gctgctcgtt ttattcggtt tgttaccggt tgccggaatt ttcaatccgg ttgctgcgga
    35701 agaatctgca cggaacgcct ccgggaatca aacggaaatc gatctgatgc ttctgtttac
    35761 caacgattta cattcttact ttctgccgca tcgcgtttta acggaagacg gacgacagac
    35821 agtgcagggg ggatatgcca gacttgcctg tatgatcaac gagcagaggc gcctgcatcc
    35881 cgctaaaacc ttgctggtcg atgccggcga tttttcgatg ggcactcttt ttcataccgc
    35941 gttcatgacc gaagctttcg aactgagact gatggggaaa atgggctatg ccgcaacgac
    36001 gtttggaaat catgatttcg actttcatcc cgagggcctg gcaaagatgc tgacagcggc
    36061 gcgttccaaa tcgcaaatac tgccgcagat tgttgcctcc aatcttattg tgaacggtga
    36121 ccatccaggc gataaaacgc tgaagcaggc actgaacgat tatccggtcc gggattacat
    36181 ggttcttgaa agaaacggtt tacgcatcgg cctgttcggc atcatgggaa aagacgcggc
    36241 cgacgatgct cctttcgcca aaccggtgat gtttgccgat ccggtggaag caagcaaaaa
    36301 gaccgttgcg atcttgaaga accgggaaaa ggctgatctg gtcatttgcc tttcccacag
    36361 cggaacgtcg gccgatgcga agaaatcgga agatgaaatt ctggcccggg cggttccgca
    36421 aatagacgtt atcgtcagcg gacatacgca cacggtcttg aaaacgccga tcatcatcgg
    36481 ccggaccatt attgtctccg gcggacgcta cggcaggtac ctcggatcgc tgggcattcg
    36541 ttttgaccgg tttaagggcg cttcattggc ttcatacgat ctaagacatg tttcatcaga
    36601 aattcctgaa gacaaggcgg ttcaggaggc aattgccggc tacgaaaaga tagtcaatcg
    36661 cgactttctt gcacactatg acctgacatt cgaagacgta attgccgaat cggatttttc
    36721 catggaatct cttgaatcca tgcatcggca tccgcgtgaa acaggactgg gtaatttaat
    36781 cgttgatgcc tatcgtgaag ccgtcaaaca ggcggaaggt cccgattacc gccatgtcca
    36841 ctttgccctg caacctttgg gcgtgattcg cgattccctg ctcaagggaa aaattaccgt
    36901 aagcgatgtg ttccgggtcc tttctctggg aatcggaccg gacaggattc ccgggtatcc
    36961 gctggtttct ttttatatca gcgggaagga actcaaggac attctggaag ttcatacaac
    37021 catagcgccg atgcataaga aagacgctta tttgcaggtg tccggcgtga aatttaccta
    37081 taatccgcgg cgcatctggt tcgaccgggt cacgtcggtt tgggttgcgg atgaaaaagg
    37141 cgtttatcaa ccgcttgatc agacaaaact ctaccgcgcc tgtgcgaatt tgtataccgc
    37201 cgcccttatt gattacgtca gctcggtcag tttcggttta atccatgtgg tgccgaagga
    37261 cagctcaggg aagcgattga ccgatctcaa ctcgtccatc gtgtctatga aaaaaaccgg
    37321 cgaaatcaag gaatggctgg cccttgcact ttatctgcgt tcatttcaag aaccgcaaca
    37381 tttgcaaacg gcgaagattc cgcttaaata cagcaaaccg gagggacgat atctcgcgca
    37441 gccgtcctgc aatccggtca gaatttttag caaaggcaat atccttacaa aggtttttct
    37501 ggtgtctgta atcctactcc ttatgatttg cggtttaatt gtctatgttt ttgtcaaaaa
    37561 aatgaaaaaa atcagcaaga agaacccccg ataaatgtca ttcccctgat taaaatagat
    37621 attgtctgta tttacaataa tttgtagaaa tattttatta ttttgacatt cattactgcc
    37681 aaaaaaaata aaaatacttg atttttgaaa attatccacg tacaattggt ccaatggtac
    37741 taccaatgtt tatatttttt gacacgctaa agatcgtttt tttgatggtg atccctcctc
    37801 aaagatagat tttccaattg cggtgccaga aggtgaagaa gaccatgcag atagaattaa
    37861 cgcccgttga gaaaatgaag gctccggagc agattgccga gaggctcctg aaatatattc
    37921 tcggaggcgg cgtcagtccc ggtgaaaaat tgcctccgga aaggacccta gcgacacaat
    37981 taaatgtgac gagggcgaca ctcagggagg ctttgaaaaa actcgagcag ttgaaactga
    38041 ttgtcattca tcagggcaag gggattatcg ttgaggattt tcacaaggcc tcaatcgacc
    38101 tgattttttc attgctggtc atcaacggcc aaattgatct caagattctg gaaaatattc
    38161 tggaagcaag agaactgttc gggacagacg ttgccagatg cgcagcccgg cgagccgaca
    38221 aaaaggatgt cgagcagatg aaaattctga tgaatgaact gatcaaggca acagatccgg
    38281 gaaaactcca gcagctggat ttcgaatttt tccggcagct cgcgctggcc agcaaaaaca
    38341 tggtgtacat tcttctgatg aataccatca aaacaatcca tgataatcat ctggatctgt
    38401 ttctgcccct gtcgacgaat ctggatacaa cgcttcagca ggaaattctg caggctgtga
    38461 tagacggcga tgaagaaaag gcggctgaaa aagccaacac ttttctcaaa gccggtatgg
    38521 aattactgaa atttttaaag tcgctttcag acacaaagga gtcccaaaat gcagaccgcg
    38581 agacaaattg acaaagaccc gatcgaacaa acccaaaaac tcaacgttct gaaaatacag
    38641 cgtacctgtg ttcatgacgg tcccggcatc cgcacaacca ttttctttca ggggtgcgga
    38701 ctgcgatgcg tatggtgtca gaatcccgaa gcgctgtctt taaaaccgat gccctctccc
    38761 gccgtgcatt atgcaattgc tgacgtcctg gaggttgtcc tgcgcgacaa ggattattac
    38821 ctcaagaccg gcggcggcgt gacgctcagc ggcggtgaac ctctgctgca ggatccggaa
    38881 agccttaccc atcttttgaa aaaattgaag gacgaaaaga ttcacattgc tgtggaaacc
    38941 tccctgcatg tgccttggga gaatatcagc aagacagcgc cctatattga cttgttcctg
    39001 gtcgatttga aggttgtggg ggatgaggac ctccacagaa aatgcacaaa gcaggacagc
    39061 cgtctcattc acgacaatat tagaaaactg cttgcgtcaa aagcgaacat taaattccgc
    39121 atggtcatgg tcccggaata taacgatcac gagcagcaca taaaggcgac ggctgatttt
    39181 ctgaaatccg taaaccacaa atcgattgaa cttttaaaat atcacaatat gtacgaagac
    39241 aaggccaagc gtctcggtct ggtccgtgaa tcgttgaata tttcaccgga gcagagtttg
    39301 accgccgtta aaaaagccgt cgaattattg aaaccattgg atatttacgc cgagtgtgaa
    39361 gatctgggaa actttcgcag taaagcggtt tttaccgatc gtgttcatgc gatacaaaaa
    39421 gatatacgaa agagcggtta tcatctctgt tttgaaacat ccaaactgaa aacaaaattt
    39481 tacagaaaaa acggtttcaa aaaaccgacc cacatccatc gcgcggaacg tctcgattat
    39541 gtgctgaaaa ataagcaggt caggatctat ccgcatgaac tgctggtcgg taattttaca
    39601 tccaaaagag tcggcggtca ggtgtgggag gaacattacg ggattctctt ttcatccatt
    39661 ctccatcaga ttcaccgcca gacaccggtg gccttccagt gttcgtggaa agacagactg
    39721 tatttttatt gcagaatatt gccgttctgg cagaaacact gcctgctgac caaagtcaat
    39781 aaaacgttgt ccgatctggt cttaagcatt tcacggttat gtgaaatgaa tgtcggattc
    39841 aacaacaata tggcagcgat agcgcatttc atcgtcaact tcgaacgtat tctgacactt
    39901 gggacaaccg gcatcatcaa agaacttgaa gaggcacaaa aagaaaagcc tcagaatgat
    39961 cagacctttt atcagggcgc cataattgcc gtcaaagcgc tggaaaattt tgcgctaaga
    40021 tacgctgacg ctctttccga catgagcaaa acagaagcag accccgtccg ccgtaaggaa
    40081 ctggaagact tggttcagat ttgccggcat gtaccgaaac atccggcacg cacgtatcat
    40141 gaagccctcc agtccatgat gtttctgcag gtcgcgctct gtattgaatc gtatgagaac
    40201 gccatatcgt tcggacgtct cgatcagata ttgtatcctt attataagaa agataaagag
    40261 gccggaatca tcacatacga aaaagccagg gaactgctgg ccttatttat cctcaaaatg
    40321 gacgaggcga ttctggtaaa cgacggagat acgtattttg ccatcggccg gcttttcgaa
    40381 acccagtcca cagaccagac cgtgacgttc ggcggtgtgg acaaagacgg tcaggatgcg
    40441 accaatgatc ttacctatat gttgattgat atttgcgaac tccagtttct cgcggtggat
    40501 atgtcggcaa gaattcatcc gaacagccct gagcgctatc tgaacagaat agcggaggtc
    40561 tatattaacg gttccccgca gccgaaactg aataatgatg aagtttatat tcaatcgtta
    40621 tcaaaacatt atgacacgtc tatcgagaat atcagaaact attccattgt cggatgcgtc
    40681 gaacccaatg cgtctgatga tcatttcggg aacaccgact gcgccaatat gaatgtcgtc
    40741 ctgccgttgc ttcaggccct caaaggcgaa gaagatgatt tatggaacta cggcttttcc
    40801 gatcaaatgg aaaaactgac gacgaaattc attgaatata atttccgcgg cggcaacagg
    40861 gtttccaaat ggatagcgga caaatacggc aaagcccggg cctcctataa aacgaaaaag
    40921 aaaacagcat ataatcctcc ggcgaatatg gatgaattgt tggaacgata tcagaatcgt
    40981 ctgaatcatc tggcatcttc catattggct gatcatcaga aaattgaaaa agaaatatgc
    41041 aaaaacttta cgacgcccct ggcttccgcg ctatccaggg gctgtgtcga aaaaggactg
    41101 gatgtgaacc agggcggatg cgagttcaac acgagcggta tccaggccgt aggcatcacg
    41161 gacgccgcgg attcgctgca cgcactcaac gaagtggtct tcaagaaaaa gatgtattcg
    41221 ttgatggacg tcatccatgc catcgataac gacttcgaag gggatgatgg tcaacaaata
    41281 cgacaagcgc tgcttgccgt gccgaaactg ggacatgatg attcccccga agcggtcaaa
    41341 tgggtcaaca agacgctgga gatttattgc aatgccctga ggcaggtgcc gaattcgccg
    41401 cgcaacggat tctataccgc cggatactat gcgctgaacg tcagcgatgt ttacggcaag
    41461 aaaacaccgt cgttgccgtc cggccggctc aaaggggttc ccctggccaa cagtatttct
    41521 ccgcactacg gcatgcagat gtccgatctt ctctcctcac tgaattccgt gtctggtgtt
    41581 gattttgtaa agtatgcgcc caacggaacc accgtcacct ttaccatgga ctcggctctt
    41641 tttcagggga gagacggcat aacaaatctt gccggtattt tcagcacgta tttcaaaaaa
    41701 ggaggcatgc agtttcagcc caacgtcatc aaccgggaaa tgctgctgga tgcttacaac
    41761 catccggaga aatatccata cctgctggta cggattgcgg gatactgcgc atacttcaac
    41821 catttgtccg atgatttgaa aaaagtcatt attaacagaa cctgttattc atgaacagga
    41881 gctgctgaaa agggcttgtt cccttccgga agtgaaacgc aaacgagtac atggacctgt
    41941 ttgcgatccc ggtcataggt gcaagtttgg tttcatgatg ccctcgagcc gtaatctttg
    42001 gcgctcgaaa tcttcctcgc tctctcccga tgccagatcc agcatgtccc cgaaacgcag
    42061 ggtaacgtgc gcaaaaggct tgggcagcat gaaatgatcc cagctgttga aataccatgc
    42121 acgatcggcg gagatataga acggcacgac gagcgctccg ctggcttgtg ccagtttaac
    42181 aacgcctgct ttcacgatgc ctgctgggcc tctgggtccg tccacaacgt gaccggcaaa
    42241 acgggattta atcagataat caatcattcc cctgagcgca cggcttccgt cttttgacga
    42301 tgatccccgc acggtatgcc agcctgtttt ctcggcaatc ctggcgatga tatcaccgtc
    42361 tttactctgg ctgatcatca gggcgggacg atagggggcg taggttttga aatggcggat
    42421 ggctgaaaaa aactgctggt gccagcagca aatcagaata cgtcccccct gtttgaggtg
    42481 atccatccag ggtttttcat tgacaacttg caggcggaat gtccaactgt aaccccgtat
    42541 aaaataataa agaaaatcct ggagaaaacc ggttaataca tatcgatatt ttttcagcat
    42601 atctatgtcc tttcaggtta ttggtaaaat caaccggtca tgaacggccg gtttgggaat
    42661 cgtctgactc cggtgttttt tatgatggga gacaaaagcc cgtcgtggcc ggctgaaaca
    42721 tcccatgcgc gatccggaga cattatggat tcccatcccg gtggttgtct acggccttgc
    42781 agaccgcgtt gttaagtttt tcattaattg aattcggaat gatatcatgc catccgtcat
    42841 tggtttccgg tacggaaaaa agcaacgtgt ctttttcatc ataaatattt atggaaacaa
    42901 ttctatattt gccctgcagg cagtccaatt ccagcaatat cgattcctga ttcaacacgg
    42961 cagggtttcc cggcgccttt tcctgcttta gcagcatggc ataagcgtcc gctctgcccc
    43021 gttcgttgta aatggttacg gtccagacct gatagatatg atcactgatt tttctgatgc
    43081 tgttcttgtc ataatagccg gtgccccggg gaagcagtcc tctgaatccc cagtccgccg
    43141 caagactccc gtatgccgat aagagcaatg cggctaaaat aacgatgcaa taaatcttta
    43201 tggttttcat ggttcgatgc aataaaaaaa tgttccggat gtttttcggg agaatatttt
    43261 tttatacttt taaatgcaag gagtttttga ggtcgctaac gccagtggaa cagtttgggg
    43321 ctgatttcgc tgcgtttctt aacgccgagg gtatgaaaaa tttttttcag atgatctttt
    43381 acggtgtatt ccgtgatgca gaatcttcct gcaatttccc ggttggagta cccggtcgcc
    43441 gccaggaaag cgatctcttt ctggcggggg gtcagatcga gtattgtgag tttctccctg
    43501 atgcatatgg tatccggcat tctttccatg acgatcagtt tcaggggaga ccgccggtgg
    43561 ttgttaatat ttttaacatg ataatatttg gccccgatgc tgaggtaggt ggaatcattt
    43621 tccgccagtg tgcgaagcag gttatccagc gaatgagttt ttaaaaccat tttggcctct
    43681 tcgttcatgt aacataaaga gggctgtttc ttttgatggt ttcttattaa aacaacgccg
    43741 gcgcccttaa tggaattcaa ggatttgatc agtgcgtctt catgacttat ctttgcggcc
    43801 ggattgagat cggatatatc cgttccaacc gtcagccagt atacctgatt ttttatctca
    43861 acggggaggg tgttaaccaa tgcgcatcgc agatcgcctt ttttatccct gacggtgatg
    43921 gtaatatttc tggcaaaacc tttttcatta atctcttcca gaattttcaa tctttccaga
    43981 ctggtggcgt atgcgatgtc agcggctgtg ttcccgatga cttcttcgcg tcgccgtccg
    44041 aaaaattccg tgaaagattc gttgacgtca acatacattc catctgtagc ccgggtaatg
    44101 gacatgggat tgagagactg ccagaataat ttattcgtga actctttcgg aatatttatc
    44161 tctgtttctt ctttcatgtt ctcatatttc tgcaatatcc ggatcatgct gttattaaat
    44221 taattaaatt attaatatct tttcgattat ctttaattat gctgctaaat tcaatggcga
    44281 ttacgctgta attgttaatt ataaaagatg tagatataat acttatattt ttcaattctt
    44341 gttttcagga tagttttgag gcgcgtaaga attgcaataa tccttcagta agtctctgca
    44401 tatcaacggc agaaatcatc gtatttttat ctgcaaggtc aggagcgaaa attgatacag
    44461 gtaaggcagt atgcattatg tgttatgtct tatcaaaaca tatatttatt ttattttttc
    44521 gtcgtgatat acttcaaaaa tattttataa acttatttag tcatttattg tgaaaggagt
    44581 ctgtcatgaa aaagagaatc atggttttat taagcattat cgtatttgtc ctcgggctcg
    44641 tctgcattca gggttgccag cagaatgaaa atccaaaatt tcagaatgaa tatcaggcgg
    44701 ttttacttac cacgggtcag gtttttttcg gaaaggttga atttgtgggt acagattatg
    44761 ttcttctcaa agacgttttc tatattcgca gcgtcgctaa tccggataca aagcaggtta
    44821 gcaataccct gataagaaaa ggcaatgaaa tacacggggc ggatcagact tacattaatg
    44881 cccgtcacat cattatgatt gagccggtat ccccgcaatc acctctcgcc ggccggatca
    44941 aggaactaca gtcccagaaa ccgcaggggc aaccgtaagt ttttaaccgc ggttccagca
    45001 ctattgttat aacaggtgtc agggtgcggt aattctgata aaaaagcccg ctgcaatcgg
    45061 cagcgggctt tttttataaa acacaacctt ttttacttca acccggcggc ctggcgaagc
    45121 gcatccaccc ggtctgtttt ctcccagggg aagcctttgc gtccgaaatg gccgtagtta
    45181 caggctttgc gatagctcca acccttcggc gtcgtgagtc caagatcctt aatcatcatg
    45241 gccggacgga aatcgaaaat ttttccttca gtgagaattt tttcgatctt attttcagga
    45301 attacgctgg taccgtacgt gcagacatta atgctgagcg gtttggccac gccgattgcg
    45361 tatgcgattt gaatttcgca tttgtcggcc agtcttgccg cgacgatatt tttagcggcg
    45421 tagcgtgcgt agtaagcggc agaacgatcc actttggaag gatccttacc tgaaaacgcg
    45481 ccgccgccgt gagaaccgac cccgccgtaa gaatccacga taatttttcg tccggtgaca
    45541 ccggaatcgc cggccggtcc gccgattaca aatcgtcccg tcggattgat gaaaaattca
    45601 gtcttgttgg tcagcatgcc cgtcttgtcg agaacctgat gtgcgacgtc aatcaggtct
    45661 ttgcgaattc ttttcagcgg aacgtccagc gtttgatgcg agatgacgac ggttgtgatt
    45721 ttcaccgcct ttccgtcttc atgaagaacg ctgacctggg attttgaatc cggccgcagg
    45781 taatctatca catttgcttt acgcaaaatg gtaagttctt ccaataagag atgtgcataa
    45841 taaatcggcg caggcatcat ttgcggcgtg gcatatgacg cataaccgaa catcatgccc
    45901 tgatcgccgg caccctgatt ttcaggtcga tcccgatcaa cgccctgttt aatatcgggc
    45961 gactgaccgt gaatggcgga aaggtaatta aatgtatccc aactgaagtt ttcacaggga
    46021 tcatcataac cgatgtcctg gacaacttgt cgcgaaatgg cctgaggatt gatcttgttc
    46081 cagtttttac aggtgatttc cccaagatta acgacaagat tgggcccgac agccgtctca
    46141 cagccgacat gggcgtgttt gtcctgttcc aggcaggcat ccagtatggc atcggaaatc
    46201 tgatcgcata ccttgtccgg gtgtccttct gaaacggatt ccgaagtgta aatgtgacgt
    46261 gtgtgcagtt tactcatgtt tttttgtaac ctctcttttc tattttattt ttttaatcaa
    46321 atatgattgg gcctcataaa aaacaaaaag cccgcgccaa agggcaacgg gctcattttc
    46381 attcacacca gcctttagca catttttaga gcggagcaat taacttgatt aaatcaccgc
    46441 ttgtcgttaa catactcatc cattgaaatt tgtcaacaat tatttctact tttttctttt
    46501 cccgctgttt ttggggaatc ggtataaaaa atcatcaatc attttggtca tgcacacgat
    46561 cctgtgttcc cacaaaaaac gaaacagacg tttatagatt gatatcacag ggattgtttt
    46621 gtagcaccat gaaaaggacc ggctttgttt ttctgcaaaa acaaagccgg tgtcataaac
    46681 catcttgggg tgaaagaaat caacatgacg cggttggccg ttgtcagtgt tgatgaatta
    46741 cgtcgtccgg ctatcgtcgg tcctctctga cgggacgtcg gtcnnnnnnn nnnnnnnnnn
    46801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46861 nnnnnnnnnn nnnnnnnnnn nnncgtcggt cctctctgac gggacgtcgg tcatcacggt
    46921 atttgatgac ggtctttgat ttttttactt cacgcctgta ttgcgtgccg gagcgccggt
    46981 ctttccagtt ttttacaccc cagctatgtt tttcccagcg tttttctttt ttccagccgc
    47041 tccagttacg ttgaagctcg tgatggctta tttttctgtg ttcccatcgg tgtcctttcc
    47101 aggtcttatt gcggtaattg tccttccacc cgggcggaat ggtacgatgg aaaaacggcg
    47161 gctgatggtc ataagtccat cccctgtcgt aatagcgtga acggtaccag cggccgttcc
    47221 acggccgcca ccaccaaccg gaatagaaga ataaatcgtc ctgaatatcg ggtaccgcat
    47281 agatgtatgt ttccggtatc accaccagat gcggcggcgc cgggaaaatg attggcggcg
    47341 gtagagggat atcgatgtgc acactgactc ccgccgatac tgaaaacggc gcaaagagca
    47401 cgcctgcgaa aaatatgatt ccggacatta attttttcat cggattacct cctccgtcgg
    47461 gtcggttaag gttggtcgaa aagtttccat cgtaagtctg atgccccaac gataaaaaaa
    47521 agacaggggc tgtcaagcat gaagcaactt cctgccgccc ggttcccgag ccatgtttac
    47581 cggtcattcg accattatga tccttgcttt gcaagccttt tccgaaactt taaataatcg
    47641 tcggcagcga ttttcggttg ctctatcatc ggcagatgtc ccagcccctt catgatgatg
    47701 atttcggagt tctgcaataa tttgtttaga atcggcgccg tttggggact gatgacgcga
    47761 tctttttctc cccagacaat cagggttggc gttttgattt ctttgacgta tctttccgat
    47821 gcatcggacg acaactgctt gaaaatacgc tgttccagct cataattcct gatgcgttcc
    47881 tgcgccatga cgtttaaaat cggtcgcgga atgaacggat gatcggtcat gacaaaactg
    47941 aatatttcag caaactcatc ttcgtttttg gccatcagcg gatttttgcc tgttttggcg
    48001 atgatttcat gcaattcacc gggcggagcg ctccagattc cgccgggatc gatcaaccac
    48061 aagcttttta cttcatcagg ataaagaaaa gcgtacatca ttgaaatgtg gccgcccatg
    48121 gaacttccgc ccacgtgcac atctttgatc cccagcgcct gcgccagggt tctgattctg
    48181 acagcctgcg ccagtgatcc gtagtcttca ttttgcggac ggctagaatc accgaaaccg
    48241 atgtggtcgg gaatgatgac cctgtaatga ggcgtcagaa acctggccac ccgcgtgaaa
    48301 ttatccttgt tcgcgccgaa tccgtgcagc agcatcagcg gctcaccttt tcccccttcc
    48361 agataaacgt aacggatatg atccgaaagg ttgatttcct ttttttccag cccggaacgt
    48421 ttgcgttcca catccagagc gagcgacgtc gcctgttcgg gagccagata aacgaatcct
    48481 atgcccgtcg ccaagcaaag aatgatcaat acaacaatcg ccttgagtag tcgcattttc
    48541 ccctcctgtt ttttgatcgg attaattctg gttatctaat atagcaatta ttggtcaaga
    48601 tttaaaatga attcttatcc gttttctgcg gatagcgatt tctcttttgc cgtgtttttc
    48661 agactgccgg tataaagcgc gtattcaagc agacggcatt cgatatcgcc gttgtacagg
    48721 atcacacgcc tttttgttct caagccgact ttcttgatca gttccagatt gccggtaaaa
    48781 atatatccat gacaaccagt acatgttttt ttgaagaaat cgccgatgcc cttataaagc
    48841 gcttccagtt ttttcatctc tcttccccga tggcggttgc cgtcgccgga cttgtcgccg
    48901 gatttacgaa gaatgatttt gcgaccgggg ccgggcgttt ctctgctgtg acggaaatcc
    48961 gctgacaccg gtttgcgagg atcggaactc atgcgttcac cgtaaggagg attaaaaata
    49021 atgatgccgt cgcccgcggg gacggacgtt ttttcgaacg gacagatatt gaattcaatg
    49081 aaatgatcca ccccggcagt ctgcgcgttt tgtctggctg ccgaaatcgc ctctcggttg
    49141 atatcagtgg cgataattct ggccggcaat tttttcaggg cattcgtccg ggctttgcta
    49201 cgcagcgtct gccagtgatc ctcggggaac cctttgatga acataaaacc gaagtgattt
    49261 ctcagcagcc ccggtgcccg atgcagcgca atggatgcgg cttctatggc cagagttccg
    49321 ctgccgcaca tgggattgat aaaattactt tctcccttcc agcctgccgc cataatgacc
    49381 gccgccgcaa gcgtctcctg catgggcgcc acgaggggta ccttgcggta tccgcgcaat
    49441 gccagtcgtt ctcccgacgt atccaggtaa acagtggccc ggttatcttt ccagtataca
    49501 tgaaccacgg ccctgttttt ttccggaccg gaatccggcc gatttccgga aatgtcgcgt
    49561 aggcgatcca cgatagcgtc tttgaccttg acattgacaa attgcgaatc tttaatgagc
    49621 gggttctcgg cgatggaggt cacgcagaga tacgccgcgg gtccggaatc atggagcaga
    49681 ttttcccagg gcagggcatg aatttttttg tagagccatt ccggcgtatc agcggagaag
    49741 gagtccaatt gatataaaac ccgctgggcg gtgcgcagat gcaggttgag cgccatcgtg
    49801 tcctgaaggg ttccttccgt ttgaatggcc gtatcccatt ctgcccgaac cggataattc
    49861 aaggcctgga cctcttcttt taaaaaaggc gatacgcctt tcgggcaagt gaccacaatg
    49921 gtatttttct tattccagat ggacatgaac tgcttctagc acaggtcgtt gtttttgata
    49981 agcgatatcc tgtaacgatt tttaacgcgg agggtcaaac cggaaaagga accgggatga
    50041 tcacagatat ttatttctaa gaaaaagatt tagtgataag agagcggccg ggagcgtgat
    50101 tgccggtgat gcaggatttt attgatgaaa aatgatcgca aaatattctg gggctggtac
    50161 attgtgggcg gcgcttttct ggtcatggcc atgaattacg gcgcgcgata ctgtttcggt
    50221 gtttttctca agccgatggc caatgaattc gctttatcac ggtccgtcat atccctggcg
    50281 gcggccatca acatgatggt ttattcattc ggatctgttt ttgtcggcag aatgctggac
    50341 cgcgttgcgc cgcgctggat cacaacggtc ggcgccggcg ttgccgcctg cggttacctg
    50401 ctgaccggtt ttgtcgatac accgatcggg ctgtatatca gctacggcgt catggtcggg
    50461 ttggggtccg ccggaatggg cgtcgttgcc tgcagctctt ccgtcagcaa gtggttcatc
    50521 agcaagcggg gtgtggccgt cggcatcgca tccatgggaa tcagtctggg aacggtcatg
    50581 ctgacgccgc ttgcgggtta tattgcaaca gtattcagtt ggcgatccgg attgatggcg
    50641 ttgagtttca tcatgctgat tgtgggcgtt gttgttgcgc agacgctgat gagaagaacc
    50701 cacccggaag cctacggtct cttgccggac ggcaatacaa tatcaggcat gcctcaggcg
    50761 gcagaggcaa cggccgtttg tccagtgtcc accatgatcc tttttcggga ttcccgtttc
    50821 tggacgctgg ccgtctgtca gggattggcc gttatgatca gcatggccgt ttttgttcat
    50881 caggtagcat atgccactga caacggtatt tccaaaatgg cggcagcctc gtctctggca
    50941 gccgtcagcc tgaccggttt tatgggacaa ttttttttcg gctggataac cgacaggatc
    51001 aatgacccca aatacgcttc tttcctcggc atctccttta tgctggcggg aacggtcatt
    51061 ctcctgaacg tggaaagcct gctgcatctt tacgttgcgg ctcttgtcta cgggttcgga
    51121 tacggatcgc ttgcgcccat gctgcctctg attattgccg accgattcgg ccggcagtgc
    51181 atgggatcga tttacggcct gctgacgttc tttatcggca ccggcggcgc catcggtccg
    51241 gttctgggag gttttattta cgaccatttc ggatcgtatc ataacctgtg gctgatgaat
    51301 attgtcgtgc tgagtcttat tgcaatggct tttctgaccc ttaaaaaggg caggccctac
    51361 gccgcttgat caaatgaaca gagggtctag cgggcgagta gaaggatttt cctgatgacg
    51421 tctttcgtgt agccttcata gcaccatact tgcgagaggg cgaaagcatt cccggcgatt
    51481 gcgtcaatat cgctttcggg gatgtcaact gcctgtaaag acaccggact gccgatcgat
    51541 tcaaaccagt gcttcaggca ggcaattccc ttttttgctg cggatatcgc gccgcgtccc
    51601 ttcacatgaa aaatttcccg ggcaagtttt tccaaccggt ctgttttttt gtccaacgcg
    51661 tacgacatcc atgccggtaa aataatggac agtccggcgc cgtgggctat atcataaatg
    51721 gcactgagcg aatgctcgat catatgcgcg ggcattcgca cctggcccat tcccgccgtt
    51781 gtgagcccgt taaatgcaag accggcgctc cacatcatat tggctcgggc gtcatagtta
    51841 tccggctcct tcaaaatgat ttccgtgctt tccatgacgg tttttatcag cgattcgacc
    51901 agcctgtcct gcagcgggct ggcggaagag gtgttgttga aatatccttc cagcatgtgc
    51961 gtaatgatat cgacggcgct gtaagcgctg taggcggggc tgagtgaaaa aagaacggtc
    52021 ggatccagta cggagacctt cggttgaata aagggagata cgatgcagta tttttgacag
    52081 ccgtcttccc gggtgacgac agcgccggca ttcatttcgg atgcactggc cgagacggtt
    52141 gccaccgtca gaacgggaag ggcgccggtg acgggcttgc tgaacgtaaa aaaatcccac
    52201 acatcgtgat gtgccacaac ccctgcggca atagctttcg cagcatcgat cacactgccg
    52261 ccgccgaccg ccagaataac atcgacgttt tccccgcgcg ccagtgcaat gcccttttgc
    52321 acatgcgaaa gcaccgggtt ggatttgacg ccaggaaaat caacgatttc gacgccggct
    52381 ttctcaagag cgtttgttac ctgatcataa acgccgtttt tcagaatcgt acccatgccg
    52441 aaaaccagca gggccctgcg tccaaaacgg gctgtttctt tgccgattcg gggaatctga
    52501 cccttgccga atataatttt ggtcggattt tcaaaaacaa aattttgcat gattttaccc
    52561 cttcctacgt gcttttgcgg tccggccaga tcctatcgcc ggcctttgag acttcttcta
    52621 caagaaggcg cccatggtta gcgcactgat gtccatgctg aaatcgagct ttgtgtggac
    52681 gagttctctg ttgcggaccg caaacggatg aagaaccaga gaataatgtt ccagcgtcaa
    52741 tttaagcgac gagagcatat ctgccagatg tcttttatca gccattaagc tgccaagcgt
    52801 gacaaggcag ctttcgaacg cttcgctcac gggcagggtg acctgatgaa tattttttac
    52861 cgggcaatcc ggcgttaaat cgtcaggcgc gggcgttacg gttaattgcc ggcaccagcg
    52921 cagaaacaag gcgggattga gcttgaaagc gggagcaaaa aagtaaaaag gtgttttgtc
    52981 ccagatgtcc aggggggctt tcggcagatt ggccacttta atgaagtctg cgtagtttcc
    53041 cagcgcgata ccgtcgacct tcaccttcag ttgccagaaa gggaggtaag tgatctcgtc
    53101 ccctttttcc cgccacgttg cgtacggaat cggaacaaaa tgctttttcg atggattcca
    53161 cgcacgatgg caattctcac aaaatacaat caaggcgtct ttggcgccgg gcagatccgc
    53221 gccgcattcg ggacaaagca ggggcagaaa atcaacatgc cacttttcgt ccggtgatga
    53281 aacgagtttg tcctccatct ccggctcttt tttttgtatg tccagaggct tctttaaaac
    53341 ggcatcgtaa agacggccgt tggcaaaata aaccggcgta tagatgatgc tggccgtttc
    53401 gccgatgaat atttcctgaa cggcgccggc ctgtgttttg aaatttttca acagttttcc
    53461 cctggtcttt tgagacgctg caatgaattt tccttcatga tctgcattgc ccacaaatcg
    53521 taatttcatc gcctgaggtc tgagccccag cgagtggggg aaaaaaggca aatcaaacga
    53581 gatgaaattg gcgtcaaaat atttataagc aatattgtgc gttgtgatgg cgtaggagag
    53641 acccttgaga cgccagtagg ggataaaata aattttttca gaaattccgg cggccggggg
    53701 gatgtaaaag agaaagtgat catcggtggc cagatagaca cgcgtcctgc agaaatggca
    53761 cgaaagaata cggtccgtct cgtcgagaat aatcggcgca ccgcactggg gacattgctg
    53821 ttcgatctga aagttagagc cgttcaccgc acttattgca atacatcgaa tccggcaggt
    53881 tttcaaaacc gcacttggcg cactgtttgc cgggaggatt tacagtgacc ggtttgccgc
    53941 aactgacgca gaaattggca ttcacaggca agtttcttcc gcaatgtgcg catttgttga
    54001 caatcagaat ctgatgaccg catgcgggac agaaatgcgc ttccttcggc acaacctgtc
    54061 tgcaatccgg acaggtgaaa acgtcggcag caacggttgt tccggctcct ttgaacgctt
    54121 ccagcagcat tccgggcatc atgaaaccca tgcccatacc caatccggcg ccggcttccg
    54181 actggttttg cgctgctgct tcaacggcca tcgccgcttt catttttaaa agcttgttga
    54241 ggtcatcaaa gacacccagg cggcttttat cgtcgatggc tttttggact tccggtggcg
    54301 gggtgatgga attgatgtac aggtcggtca gtgccagacc gaatttgctc agatcctttt
    54361 gcagcatgac cttcagacct tcggaaaact cattgtattt tcccggcaga ttgaaaatcg
    54421 tgtccacctt ttcgcccata taatcgttga agcgtgaaac aatgacacgg ttcagatatt
    54481 cttcgatttc ttccgtgctg aaaatacctt gtgtacctac cagggagtta atcagcagaa
    54541 gcggctggat gacacggatg ttgaaaacgc cgaaagcacg caggcggatc agccccagtt
    54601 cccggtcgcg gaaagccacc gggtcccgtg tgccccattt taagttgggg aataccttca
    54661 tgttgatgaa gtagacttcg gcgcgcagcg gactggtcat tccccaggga atggcaatga
    54721 ctttggtcag cagaggaatg ttggcggttt tcagtgtgtg tcgtccggga ccgaaggcgt
    54781 cataggcctt gcccttgtag aaaaagacgg cgctctgact ttctcggact gtcaattggg
    54841 cgccgaattt tatttcgccg gagccctttt ccggaatcct gtgtacgatt tcttttcccg
    54901 attcgtcaaa ccattcgatg acttccagaa aaattatgtt gtttgttccc atgtcaatcc
    54961 gcatccttta ttttgtggcg attattgaaa ttcaacgttt cagcgtgtaa aaaatcataa
    55021 gggagggcaa ccaaatagtc aagaacaaat gaaggattca atttgatttt ttaaacggga
    55081 tattatagag aaatcatcat gaaaaaaacg tctgctgagc aaaatatata tgacggtctt
    55141 gcgcttattg ccgatccgat tcatcagtat attcttttta ccgttccaaa ggacaagtct
    55201 gccaagtctg ccggagaaat aacggagcag catatcattg attctccctg gatgcagagg
    55261 ctcagaagaa tttatcagtt acagagcgcc cgctgggttt atccggcggc ggagcattcc
    55321 cgttttcagc attccctggg cacgatgcac atggcgggtg aatttgccaa aagcatttat
    55381 ccgagcctgt cttccgtctg caaagatgtt ccgtcctgtc attacgtgga agaattactc
    55441 cggctggccg ggcttctgca tgatgtcgga cacggccctt tcggccactt ttttgacgat
    55501 aattttttga gcaactggag tctgacccat gaaatcgtgg ggcagaacat tatcgtggac
    55561 aagctcggcg atgatcttgc gcgcgtccgt cgaagccctt ccggcggatt ttcacgcaca
    55621 gaaagaatgg atgcgcaaaa agtggcgtat ttgatcagga tgcccgaaga cggtgaggag
    55681 ggcaggccgc cgcgctggct gcaaatgctc cggcagttgt tttcgggtgt ttatacagcg
    55741 gacaatctcg attatgtgca gcgagacgct tacatgaccg gattctccct ggatatggtg
    55801 gatatccccc gtttacgcta ctacactttt tttacccggg aaggtctcac gcttcatcaa
    55861 tcgggcattt ccgcactcag acggttcctc aacgcccgtc tcaatctcta caccaacgtt
    55921 tattttcacc gcacgacacg ggggctggat ctgcatcttc aggaattttt tcatgatacc
    55981 atgcagatga ttttacccgc caatccgctc aacaatcttg acgattatct gcaatgcgat
    56041 gaatggacgc tgtttaatca tgtgcagaac tggctgcgat ccaaagacga acaaaagaaa
    56101 aaactggctg ccgaatggaa aaaacttcac gaccgcgaag tgaaatggaa aatgtctttt
    56161 gttgcagaaa tttcactgga tcagatacag cccggggttg gcttttcccg ggcagccgat
    56221 tatgaacaac agatcaggga acgccttccg aaaccgctga aatcccttcc tttcaaggtt
    56281 gatctggcga cgcaggatcc gcgtccgctc aatccgatta ccgagaggga aaagagaatc
    56341 aatattttta atcccgtcac acacaagacg tctcccgaac cgcttctcga aatttaccgt
    56401 tttattccgg cacgggtcat gcaattcaga gtttttgctc tgaaccatga tcatgacgga
    56461 gacctggccg ctgccgctga aaaagttctt ggaagctctg caaaatcaac actgacgaat
    56521 atctgacatg agtaaaaacg acgagacaac agacctgact ctttttgtgc ggacaacggg
    56581 agaggaaatc gttctctggg accgtcagcg tattgtggat gccctggtgc gcgaagccga
    56641 tatcgatgat gacgtggcag gccagatttc gaaagaagtg gaaaaacaga tcattgtatc
    56701 gggaatcggc ctgctcacaa cggccctgat ccgtgaactg gttaatgtcc ggctgatcga
    56761 aagaggcttg gagaaagagc gccggcttca cggccgtctg ggattccccc tttatgatgt
    56821 gcgccagttg atcctgcatc ccaacaaaca ggacgccaat atcccgcatt ctcccgaagg
    56881 cacgaatctg atttttgccg aaggcattaa acgggaactc tctttgtttg acgttttttc
    56941 cgccgatgtc ggcgaagccc atgccacggg agatattcat atccacgctc tggggtatat
    57001 tgaccgcccg tatagctgct gccagtctct ggaatacctc aaaatcaacg ggttaaatct
    57061 gcctcatgcg atcaattccg ccaatcccgc caaatacgcc gaagtgcttc tggcacatat
    57121 ggtacgcttc agcgctgttt tgcagggtca ttttgccgga acggtcggct gggatgcggt
    57181 caatatttca tttgcgccgt atctggcctc catgaccgac aaggaagtca aacagtttgc
    57241 ccagatgctc atttatgaat tctcgcagct cgccgcaacg cgcggcggac aggcgctcta
    57301 caccgacata cacatttatt atgatgtccc gccgcactgg gcggaccttc ccgcggtggg
    57361 agcggaagga aaaccgacgg gaaaaacata cggcgattac acgtgggacg cccagcgctt
    57421 tgccatggcc attctggaaa tttttgccga aggggacgcc cgaggaaaac cgtttattct
    57481 gccccgtccc cttttgcata ttacggatca tgtctggaaa aacgataagg cccgtgaatt
    57541 tctgcttcgt gcctgcgatg tggccgcgct gatggggaat ccctgttttg tttttgacag
    57601 ggatgaaaaa gcggtcgacc tgattttcaa cgacgaggag aggcgcaaac cctggcttca
    57661 gcgccgggcc gctattcaga cggtaacgct gaatctgccg cggctgggtt acaaaaccgg
    57721 tggagacgaa aaagaagtct tttccctgct tgaagccttc atggagaaag ccgttaaagc
    57781 ccatgtgcag aaaaaagatt ttctggaaaa attgctttcc tatgccgaag agggacctct
    57841 ggccgtactg gccatgaacc acgacggatt tcccttttta aggatgcatc tggccagcta
    57901 tgttatcggc attgtcggtt tgaatgaact ggtgcagatt gttaccggaa atgaaatgca
    57961 tcaaaaacag gaggcgttgg aattcggcct gagagttgtt gatttcatcc ggcaggaagc
    58021 gcttcgttta agcagacaat gcaaaatgaa attcgttttg gggcagtctc tggcggaaac
    58081 caccgcgtat cgttttgccc gtcttgattt aagatatttc tctccgacag ccggacgtta
    58141 tgtcaaggga gatattgccg agggagccat ttattacacg aattcgacgc atttgaatgt
    58201 tgcagccgat gttgggcccc tgcagaaggt gatgcgggag gggcttttcc atcattacct
    58261 tgaagacgag gtgattacgc atttgcagct gggagatttg aatccgggaa ggaacgtcct
    58321 tgcccggttc atccgcgacg tattctatca ttccgcaaac cgccagatag atttcaatcc
    58381 ggaattcacg ttttgtcttt cctgcaagga aacgaccaga gggttgcagg acaaatgtgc
    58441 cgcctgcggt tctgccgatg tggagggcat cgcgaggctc acgaagtatt tcagcaaaac
    58501 ctccggctgg aacaagggga aactcgccga gctgaaagac agaacaatga atgacaactt
    58561 taataatgtc gatcagccga aatgttaaga cgaagctgga ttgtccgcgg catctcgaca
    58621 gttttttctt aatacaaaaa gcatgtattt cttttcattt ctaaatttta tcgggtcaac
    58681 ggccgccagt tccttccata ttttatgatc aacggattgc tttaaatctc tgatgaggta
    58741 gtataaccat gcctttttac gcttgatttt tccggatgta aaatggacca ggggtaaaag
    58801 aaatcgttca gcataattat agacaaattt cagcgtcggc aaaacatgag gcgtcatgtc
    58861 ctgctgatac gtaatctccc agccatgttc ctttgatttc cgaacaaaat catcgagcag
    58921 gtgtgatgac ttgttgacgc ctgaattatc gaatttgaaa tagtccgcaa taatccatgt
    58981 gccgtcaggt ttgacaatgc tttccgcttt ttcgaacgct ttctcaagcg gtatatattg
    59041 aagcgattcc gaattgatga tggttccata ctttttatcg gtttcgaatt cttgaaatcg
    59101 cttctgataa cacgtaatcg tcgggtactt tgattggata tgcctgatct gatttttatt
    59161 cggagtcaac gcctcaacat taaatccctt ctgggatatt aagttcgaca agccacccat
    59221 tccgcagccg atgtcaagaa ttaaatcttg cgggttgatg atttgatcga tgatgttctg
    59281 cgcatatctg acctgggctt tttcaatttc agcgatagag atggtgtcga cttcaatctc
    59341 ggtattttcg aaatacccat aatgaagcat gtcatttttg aatattttcg aataaagatg
    59401 aagttccagg tcggaagttg agttgctgaa ggttgaattt ttcttgtggt aaagccaggc
    59461 gttaaaaaga acaacaggat ttataatttt taaaagtatt ttcttcatta tttgcgggag
    59521 ggactatatg ggaaacatta ttctatgtca atgacaaact aaccgacgaa agtcggcgat
    59581 tgaattgaat tgatattgat tgaattgatt attgattgat aattattgat tgattgaatt
    59641 aa
//
LOCUS       DAOOLB010000004        59443 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_337_length_59443_cov_45.925507, whole genome shotgun sequence.
ACCESSION   DAOOLB010000004 DAOOLB010000000
VERSION     DAOOLB010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 59443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59443
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_337_length_59443_cov_45.925507"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PLF77_00985"
     CDS             <1..321
                     /locus_tag="PLF77_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07457.1"
                     /translation="IERSDDGKIIGLRTIAGPNSTEEKSINDQEVIDFLKDNLDNNFL
                     KNLLVHSDAGLIRLLEDLIALLTKKNIILFTELPEKAQAKIIEREKIREKISSDNIIV
                     EDIL"
     gene            612..1967
                     /locus_tag="PLF77_00990"
     CDS             612..1967
                     /locus_tag="PLF77_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HPC07458.1"
                     /translation="MKPYIKNVFILVVILLFAGTTGVRGETLRDSVKSVLQNNPDVTS
                     VAYNRLARDQEVRQAMADFFPRIETTLSDGYIDQHHPYLLDDEFHPRSATVRLNQNIF
                     RGGATFAETKRQKSRVISAAYQLQGVSENTALLACRVYLNYLRAIEQDNLAKENLLIH
                     ERFYDQIKLRSQAGVDRRVDLDQITARLALAKSNLIVTQANIENAKTDYQAVMGYLPV
                     NLVKPEPAYAEIPATLQDAERIALASHPTLKSAKADIEARNYQHKTARALAYPTVDVG
                     AGYTWGEDLNRPAASYDYEDYLQLNATLSFNIFNGFINQARIKETSYLINEAEEIAKK
                     TELQTIQSVRLSYESYLADQRRVRELEQYVDSTDKAAEAFISQWSIGRRSLFDVLDSS
                     AEKITAKSDLINAQYDKMYSAYRILSGLGNLVHTLGLQWPEESRVDTGAGIVDMSFLE
                     K"
     gene            2168..3040
                     /locus_tag="PLF77_00995"
     CDS             2168..3040
                     /locus_tag="PLF77_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HPC07459.1"
                     /translation="MKYWKITGVISAALCLLITHQAWADREWVCLESNSSRESFYDKN
                     SIVKVDENVFRLWMKQVLSENGKIKTISKFRNTNKPDNSLDLISYILRQMDVDCRNNQ
                     YKDSALIFYDEKSNVLYSSPKGAIGKWDDIVEGSFAYQLKNVICKGPNLAEKPSAILS
                     ADHAKKPAEAGTPSQSLPAGTSDEDIRQVVSKWLAAWQSGDIATYRSCYDEDFKSKNM
                     TLEKWIAYKTGVRQKSTDIKIGIDDLKIIADGDHAEAVFIQTYSSSLLNDKGKKTLKL
                     KKVGNEWKIYQEIM"
     gene            3361..4233
                     /locus_tag="PLF77_01000"
     CDS             3361..4233
                     /locus_tag="PLF77_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC07460.1"
                     /translation="MDYEGFIIRPPSEAYSLLLQVTTGCSHNKCTFCGTYRQKKFKIK
                     PIERIKKDLDEARQYEDVDRVFLCDGDALIIPQPKLKEILELINENLSDIERIGTYAN
                     AKSILRKSVEELKELKSLGLKIVYLGVETGNAELLKKIHKGVTYEQMIQAGRRIKEAG
                     IILSVTVLLGLGGREKSIDHALDTAKILTAMDPDYVGALTLMLIPETELYEDYLAGRF
                     VLPDQFGFIRELYLMIEKSSFTDCYFTSNHASNYLPVKAHLPREKEKNLKMIGSVIHE
                     KDISRLRPEYLRGL"
     gene            4250..5083
                     /locus_tag="PLF77_01005"
     CDS             4250..5083
                     /locus_tag="PLF77_01005"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPC07461.1"
                     /translation="MNIKKNISKLFFWREQKYIKNKINAMLSVAQKSGLVDASSREMI
                     ENILEFSHTLVREIMIPRTDIVAVSTEAAIDDIIHEVIASRHTRIPVYRDTIDNIIGI
                     LNVKDILMFWSKPMTKEDLIACLSTPHYIPETKNTHLLFYELKENKKHMAIVIDEYGG
                     TAGLVTLEDMLEEIVGEIRDEHDGNVTDDRIIQSPDGSLIFDGRMEIEKIEENLNIDL
                     KKGRYETLGGLILDAIKRIPVSGEKFQIEGLDVTIDKADERSIKKVKIKKIETDNDDE
                     K"
     gene            5073..6635
                     /gene="lnt"
                     /locus_tag="PLF77_01010"
     CDS             5073..6635
                     /gene="lnt"
                     /locus_tag="PLF77_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPC07462.1"
                     /translation="MKNNFPEVSLADISRNLFRKNIFFAALSGILLFLSFPKYGSGVL
                     AWVALVPLFYALKNAATIRHGLLLGFITGIISYVGIIYWIAHVIVNYGHLPLYIGIAL
                     MLLLAAYLSLYLSFFAGCLVSFREKVPFYIIGPALWVSLEFCKSFILTGFPWENLGYS
                     QYGNTYLIQVADIFGVPGLSFLIVLVNAVLLDVIHRRTKKEYVAAGMVFVLIAGIYVY
                     GFFRLREIDATVTSAPGIDVSLIQGNIDQAVKWDQDYKQETLDIYERLSLKNKPGRGS
                     LIIWPETAVPFKFQNINRFRRQIIDLSLKTQSWLLFGSVSYEPGAYGNDFFNSAYLLS
                     PSGDVRGRYDKVHLVPYGEYVPFRDLMPFVKGLTSGIGDFSSGAGFDPLSLNDKKIGV
                     LICYEGILPFASRAYKNKAAELLVNITNDAWFGATSAPFQHFSMAVFRAVEMRLYLVR
                     AANTGISGIIDPAGRIIAKTGIFQEEALKGHAKFVTIPTVYERVGDIFIIGCFMMVSI
                     CFIFSLRRRSQK"
     gene            6632..7739
                     /gene="prfB"
                     /locus_tag="PLF77_01015"
     CDS             join(6632..6703,6705..7739)
                     /gene="prfB"
                     /locus_tag="PLF77_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139198277.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPC07463.1"
                     /translation="MTTENILETISDLKRRIQHIGECLDVPELKMKIKDLEELSLKKD
                     FWNDQDYARSVLQKKTNLSEKVERWDRFSKDISDIENLAEMAVREKDEHVLQDIDEEL
                     EQLNAAVAQEELKMMLNGEQDAMNAIVSIHAGAGGTEAQDWTEMLLRMYLRWAEKKKF
                     AARIIDYLPGDEAGVKSVSFTLEGEYAYGYAKAEIGIHRLVRISPFDAGARRHTSFAS
                     VFVYPEVDDDIKIEINDDDLRIDTFRAGGKGGQHVNKTDSAVRITHVPTGIVVQCQNE
                     RSQHQNKAMAMKFLKSRLYERELQLKNQKIDEENKQKKDIAWGSQIRSYVLHPYKLVK
                     DHRTNVECGNAGKVLDGDIDEFIQAYLMMTTTTS"
     gene            7909..9429
                     /locus_tag="PLF77_01020"
     CDS             7909..9429
                     /locus_tag="PLF77_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPC07464.1"
                     /translation="MTGLTRNRLIGLIALICLFIVPLIGYAENSIDKKSPESSGDITK
                     QKIDQKIKLAALNNDDESSLFDFPQENDPLADVQEDDEKQDMMENALELLGEADKLWK
                     NGDIEETLDTLDEAYSLILNANGDSAIAQEKDDLRLLISQRILAVYSSKRDVLQGKNG
                     EIPLLMNGDVQKEITSFQTVERDSFLAAYERSGMYRSEIVKELKRSGIPEEFFWLPLV
                     ESLFKINAYSSARALGLWQFIPSTGYKFGLNRDEWVDERMDVQKSTKAAIAYLKELHG
                     MFGDWLTALAAYNCGEGRVLRVISRQRLNYLDSFWDLYRQLPNETARYVPRFLATLHI
                     VNNPQKYGFDLSHTARPIEFETVRVNRIMKFNDIAERIDTTEDILCLLNPELRHKMTP
                     DREYDLKVPGGMLDKFSFVYHEIPSSEKPSIASVQTYVKASDRTRRTYIKHRVKRGET
                     LYSISRKYKVSISSIRSLNKLSPKKKLIQGRRLLIPVASAKRHAKGLTVKKKFKKK"
     gene            9435..9836
                     /locus_tag="PLF77_01025"
     CDS             9435..9836
                     /locus_tag="PLF77_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPC07465.1"
                     /translation="MGRYKVKKGENLSTIACRFSVSVSELKAINNLSSGQIYAGQRLK
                     IPQKEDGKDSGNEDSEKNLRTSAQKSKIDKKILSAKDINQLGPDKYIVTKNDNLHTIA
                     KKNNTNAARIMELNGLSVDAKIVPGQILVVQ"
     gene            10024..10887
                     /gene="panC"
                     /locus_tag="PLF77_01030"
     CDS             10024..10887
                     /gene="panC"
                     /locus_tag="PLF77_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPC07466.1"
                     /translation="MNVVKSVQKMQSFSENLRLEGKKIACVPTMGYFHEGHLALMREA
                     RKQADCVVVSIYVNPTQFGPQEDFSKYPRNFDRDMKMAQNVGVDVIFYPSNRAMYPPH
                     YQTYVAVEKVTQNLCGASRPGHFRGVTTVCNKLFNIIKPHVAVFGKKDFQQFVVVQRM
                     VRDLNMDLEIIGYPTVREADGLAMSSRNKYLSAEQRTSALVLFKSLQLAQKLYAKKET
                     SPRIIIDAVKKMIQKTPLTQIDYVKICDTTRLQDIAKITDQTVIALAVRIGSTRLIDN
                     HVFGEALKISY"
     gene            10912..11268
                     /locus_tag="PLF77_01035"
     CDS             10912..11268
                     /locus_tag="PLF77_01035"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPC07467.1"
                     /translation="MQRFMLKSKIHRATVTDADLHYEGSISIDEKLLEAASILPYEKV
                     AIYDVDNGERFTTYAIKGKRNSGIICLNGAAARKVAKGDLIIIASYVVVEEAETKNWK
                     PTCVFVDGKNKIKKKH"
     gene            11464..12888
                     /gene="ahcY"
                     /locus_tag="PLF77_01040"
     CDS             11464..12888
                     /gene="ahcY"
                     /locus_tag="PLF77_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPC07468.1"
                     /translation="MAFLEIDPALKYKVADIELADWGRKEIKLAENEMPGLMALRKKY
                     GQKKPLKGMKIMGSLHMTIQTAMLIETLKTLGAEIRWASCNIFSTQDHAAAAIAKKGS
                     AAVFAWKGESLEEYWWCTEQALTWPDGSGPHLIVDDGGDATLFVHQGVKVERDPALLK
                     QKIDNKEFKIILDRLEKSFRRNPKKWQKIASTIKGVSEETTTGVHRLYQMQAAGELLF
                     PAINVNDSVTKSKFDNLYGCRESLADGIKRGTDIMISGKIVVVCGYGDVGKGCAQSMR
                     GFGARVIITEIDPICALQAAMEGYEVRTMEEVAPIGDIFVSATGCCDVITGRHMEMMK
                     NEAVVCNIGHFDSEVDMHYLETSKVCRKETIKPQVDKWTLKSGRSIIVLAEGRLVNLG
                     CATGHPSFVMSNSFTNQCLAQIELATKKHKIGVYTLPKVLDEEVARLHLERLDARLSK
                     LTKKQADYLGITVDGPFKPDHYRY"
     gene            complement(12923..14002)
                     /gene="lptG"
                     /locus_tag="PLF77_01045"
     CDS             complement(12923..14002)
                     /gene="lptG"
                     /locus_tag="PLF77_01045"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HPC07469.1"
                     /translation="MKLLDKYILKEFLKFFTITFLAFIALFLIVDFFERIRMFLSHQA
                     TAAQMASYFIFSVPMIISYVLPPSILLATLMTFSSLTKFSEITAMKANGINIFRMALP
                     IVMFASFTAVFYFYFTELITPAAVQKTTYIKNIEVKKQKTPGFFKQNEIWYRSDNAIY
                     NFKMFDIGKNILHGVMITYLNKDFTLISRIDARRAEWKNGRWVFHDLLTTRFDDNRAP
                     VLERSKEKIIPLPEKPEDFKVMQKDVEKMGYFELRRYVQKIQSEGFDARKYNVDLHGK
                     TAFPFVILIMVLIAVPFALRSERSGGVMQSIGIGIFIGFSYWIVHAFCMSLGKSEMLP
                     VVLAAWAANILFGAAAAFLLYKVNT"
     gene            complement(14054..15169)
                     /gene="lptF"
                     /locus_tag="PLF77_01050"
     CDS             complement(14054..15169)
                     /gene="lptF"
                     /locus_tag="PLF77_01050"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HPC07470.1"
                     /translation="MRKIIDRYIFKEIAFPFILILCVLTFVLLMGKILQIMDLFINKG
                     VSIFSIAKIFIFMLPYFMLFTIPIALLISILIAIGRLSSDNEITALKTSGVSMARIYY
                     PVAVASLLAFVFTLLVSYFVAPQSNFATKRLLFDIAQQNASVGIKEKVFNDSFKGFII
                     YTNKISEGNHALEEVIVSDKRTIGEQNTIIAPRAFLVSDPDTMRVKLRMENGSIHTVS
                     SDLKNYRKIDFQSYEINLDLYAALAEFDKASKAAKDMTMTELLQNMKKPGMSEKDVRE
                     LSIEAHKKFSIPLACIFFGLLALPLGMRSQRSVKARGFAVGLITVCLYYLLRIGGEAL
                     AETGYIPVAVGVWIPNISFAFIGIFMFYITSKEIYLF"
     gene            15473..16708
                     /locus_tag="PLF77_01055"
     CDS             15473..16708
                     /locus_tag="PLF77_01055"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPC07471.1"
                     /translation="MKVDTMRRIDFYAGVPLCFFGSLFYKAVKWLSPRTKKDPKNVLF
                     IELSEMGSAILVDPAMRKLRDTTGAQLHFAIFKKNKPSLDLLQTVSPDNIYTIREDRL
                     SSFLMDTFKFLHWTRKRKIDTVIDLELFSRFTALLTGFSGASNKAGFHAYFNEGLYRG
                     DFLSHKVAYNPHMHIAKNFIALVNALLSPKEEIPYSKTRIQDAEISMAKVHITDTQKK
                     TVLDIIRGCFDAFDPSRHKIVLINPNASEMLVQRRWPQAYYGHLIKMILGQCSHAVVL
                     ITGDPREREEAERLKEQVQDERCVNFAGKVSFAQLPQLYSVSEFMVTNDSGPPHFAAI
                     TEMPTYVIFGPETPALYGSLGRTTPIYAGLACSPCVSASNHRKTACTDNVCLKVITSS
                     QVFETIRPVLDRINAETLL"
     gene            complement(16720..17010)
                     /locus_tag="PLF77_01060"
     CDS             complement(16720..17010)
                     /locus_tag="PLF77_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07472.1"
                     /translation="MMRRCHKCLNEISDNVYTGRQSQCPVCSADLHCCLNCSFYDAGA
                     YNDCREPQAERVLDKARSNFCDFFRFRQTETLSDAPSQNTPGDKLEALFKKS"
     gene            complement(17108..17470)
                     /locus_tag="PLF77_01065"
     CDS             complement(17108..17470)
                     /locus_tag="PLF77_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07473.1"
                     /translation="MTNDKEYRLSASINDGIIEIILSGTVTRTNVSRLQKEIDSLRNE
                     KGSRLLIDVRTLNEGSGYHDVLYYIRRPDKATGKTAILDVPGNDRLETFLKTATLNTH
                     LEARYFSNLDLARNWLKT"
     gene            complement(17700..18932)
                     /locus_tag="PLF77_01070"
     CDS             complement(17700..18932)
                     /locus_tag="PLF77_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HPC07474.1"
                     /translation="MNPTQPSFLKSYGFSILLIASIVIGAVLGFVFKADAVRLKPLGD
                     IFLNLIFTAIVPLVFFSIASAVAGMQNIRRLGKIMSAMIFVFIATGLIASAVMIAAVI
                     FFPPAAGLNIGLGNGVDVEHLKASDQIVKAFTVGDFTELLSKKNMLALIVFSVLTGLA
                     ASVTGERGKPFANFLNSGSAVMMKVISFIMYYAPVGLCAYFAYLVGVFGPQLLDSYAR
                     AMMVYYPTAFLYFFIAFSLYAFIAAGIDGMRRFWKNIIPPSLIALATGSSMAAIPANL
                     EAADKNGVPRDIREIVIPVGATMHMEGSCLAAVLKIAFLFGLFNMPFSGAETLLTAFG
                     IALLTGVVVSGIPGGGTIGELLIISFYGFPMEAFPIITMIGTLVDAPATMLNAVGDNV
                     SGMLVARILDKKNPVAPD"
     gene            complement(18937..19704)
                     /locus_tag="PLF77_01075"
     CDS             complement(18937..19704)
                     /locus_tag="PLF77_01075"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPC07475.1"
                     /translation="MKIRFLLAVTVSFSFFYFASAAHCSDLNKKPDRFVDIQTVIPGA
                     LLDIRYHGAHNFLGERVDGYLAPKCYLTKEAADALALVQKDLEAYSLTLKIYDCYRPQ
                     RAVHHFVRWAKDIENTKTRKEFYPTVDKRNLFTDGFIADRSSHSRGSTVDLTIVLLSA
                     PPQPDYTPGQTLSECYLPSGKRFADNSIDMGTGFDCFHELSHTANAKIGEQQKINRLL
                     LKSLMEKHGFKNYDLEWWHYTLKNEPYPDTYFDFIIE"
     gene            complement(19725..20318)
                     /locus_tag="PLF77_01080"
     CDS             complement(19725..20318)
                     /locus_tag="PLF77_01080"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPC07476.1"
                     /translation="MSRPHQPDGSSQVLLVQRCGFWIFTRTIVYVMEQREGNWSAVSP
                     PMDAVIGRNGFAPPGEKREGDGKTPSGIFPLVMAFGYADSANTKMLYRQIKEDDIWVD
                     DSEAADYNRWTKQSVTQSTSYERMKRDDDLYKYGIVIAYNTNPVVQGRGSAIFVHVWK
                     DKSVPTAGCVAVSEENILRLLHWLDPAAAPLIIMGLK"
     gene            20614..21510
                     /locus_tag="PLF77_01085"
     CDS             20614..21510
                     /locus_tag="PLF77_01085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPC07477.1"
                     /translation="MSDNFFTFMIIPNRKSGVKKISVPKVAIRNILIGAIVVVLIALF
                     VIYDYASIKRDRAELARLRAQTKEQSRQLQDMGMKIDQFADRLEALRQYDKQIRVLAH
                     ETNLSKKMPLGVGGSEEVRMKDLVDKDHEQIIAGMRRNFEKLHEEANERERSFTELLG
                     YLGQQKAVLASTPSLWPVKGWVTSEFGMRRSPFRSGMEFHKGLDISTRFGKEVVAPAD
                     GLVISSGYGPQDGNYIKIDHGHGFTTAYLHLSRMTVKQGTRVNRGDVIGYVGDTGRST
                     GPHLHYAVLVNNVEVNPRKYLR"
     gene            21621..24149
                     /gene="secA"
                     /locus_tag="PLF77_01090"
     CDS             21621..24149
                     /gene="secA"
                     /locus_tag="PLF77_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPC07478.1"
                     /translation="MLDNILKKIFGTKNDRELKRLSLLLREINNLEPAMTALNDEELK
                     AKTPYFREKLKNGFSLDDILTEAFATAREASRRTLGMRPFDVQIIGGIALHEGKIAEM
                     KTGEGKTLAATMPLYLNALEGKGCHVVTVNDYLARRDAEWMGPIYKFLGLTVGVIIHG
                     LDDPERREAYHCDITYGTNNEFGFDYLRDNMKFTLDDYVQREFNYAIVDEVDSILIDE
                     ARTPLIISGPSEESTDKYYKINQIIPRLKKDKDYSIEEKSRTVVLTEDGVAHVEKLLN
                     VQNLYEPRNIEIVHHVNQALRAHTLFKRDVDYLVKDGQVVIVDEFTGRVMPGRRYSDG
                     LHQALEAKENVKIERENQTLASITFQNYFRMYKKLAGMTGTADTEAAEFKKIYNLDVL
                     VIPTNMPMIREDHNDLIYKTEREKINAVIGEVKTLNKERRPVLIGTISIEKSEMLSKH
                     LLRAGIKHHVLNAKNHEKEAEIVAQAGQAGMVTISTNMAGRGTDIKLGEGVAELGGLH
                     ILGTERHESRRIDNQLRGRSGRQGDRGSSRFYLSLEDDLLRIFGAERISGIMDKIGIE
                     EDQPIEHKYISRAIENAQKRVEGQNFDIRKHLLDYDDVMNRQRKVIYEQRKKVLRGDN
                     LWADTEEMIEEIIDNILSEFVDEKTKKEDINFKGLDDALLKQFNIRFDLANTDQTGDG
                     QSAIREWIIQGVQKYLRDKEKEFGPELMNYLMKVIMLQAIDVQWKEHLLAMDHLREGI
                     GLRGYAQKDPVREYQREGYDMFMEMIARIKMDVLEKLCLIRIQREEEVEAIRQKQKQD
                     YVMSRGEDTPEKKTVKHEGPKTGRNDPCPCGSGKKYKKCCGASV"
     gene            24168..25367
                     /gene="argJ"
                     /locus_tag="PLF77_01095"
     CDS             24168..25367
                     /gene="argJ"
                     /locus_tag="PLF77_01095"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HPC07479.1"
                     /translation="MIKNKLKISVPGFLAGGISVGIKKKDQKDLGLIYSTVPARAAAM
                     FTKNTFKAAPLLINEDRVKRGIAQAIITNSGCANAATGKEGIRDALIVSGALSRELAI
                     REELILLGSTGVIGKRLPVKKIEGGIGKLVKGLRETGIEDAEKAMMTTDKYPKIAIRK
                     GVIGAKDITICGIAKGAGMIEPNMATLLTYVMTDALIDYRALNTVFHQAINATFNSIS
                     VDGCMSTNDMAIILANGLAGNNPLEKVQARLQLFRDMLTEVLMELCQAVVKDGEGATK
                     FISIMVKGARSKSDARCAAYAISNSNLVKTACFGEDPNWGRIIAALGASGISMDKEKV
                     VLTIADLAVFSQNTPQEVLMSRLKDAFKKDRIDIEVDLGAGDKSYYVYTSDLSHEYVK
                     INAEYST"
     gene            25555..26370
                     /gene="rpsB"
                     /locus_tag="PLF77_01100"
     CDS             25555..26370
                     /gene="rpsB"
                     /locus_tag="PLF77_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPC07480.1"
                     /translation="MSAISMKLLLEAGVHFGHQTNKWNPKMKPYIFGARNNIYIIDLQ
                     QTVGLFQSAYNFVIDTVSEGKEILFVGTKKQSQEAIKEEATRCGMPFVNQRWLGGMLT
                     NFVTIKKSIDRLNSLTDMFNDDSIKAFPKKEITKLQKEKEKMENVLGGIRTIKSLPAA
                     VFIVDPGKEHIAVNEAKKLRIPIVAIVDTNCNPDDIDYVIPGNDDAIRAIKLFTSKFA
                     DAVLEGKKRFEEKLAAESNKEADAAVETKPVEADIPESLEIDDSIDKSETYAE"
     gene            26420..27025
                     /gene="tsf"
                     /locus_tag="PLF77_01105"
     CDS             26420..27025
                     /gene="tsf"
                     /locus_tag="PLF77_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPC07481.1"
                     /translation="MEITSTMVKELRTKTGAGMMDCKQALAESDGDFEKAIDFLRKKG
                     LSAATKRSSKAAKDGTIASYIHMGGKIGVMVEINCETDFVAKTEDFQTMARDIAMHIA
                     ASNPLYVRPEEIPESALEREKEIYRSQLREEKKPEKIWDKIIEGKLKKYYEDVCLTQQ
                     KFIKDTDITVATLINNMIAKTGENMVIRRFARFQLGEEIKQ"
     gene            27025..27750
                     /gene="pyrH"
                     /locus_tag="PLF77_01110"
     CDS             27025..27750
                     /gene="pyrH"
                     /locus_tag="PLF77_01110"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HPC07482.1"
                     /translation="MSTKQKAAYKRVLLKLSGEALMGKQSTGVDPDVANYIADEIKSL
                     SRMGVQLGIVIGGGNIFRGIEASARGMDRTTADYMGMLATVINSLALQSALEERGLPT
                     RVQSSIEMHEIAEPFIQRRAIRHLEKGRIVIFAGGTGNPYFSTDTAAALRAVEIRADV
                     IMKATKVDGVYNADPVRDKKAVKFNKISYIDVLTKNLKVMDSTAISLCRDNLLPIIVF
                     NLQKKGNICKAVCGQKIGTYVGE"
     gene            27753..28310
                     /gene="frr"
                     /locus_tag="PLF77_01115"
     CDS             27753..28310
                     /gene="frr"
                     /locus_tag="PLF77_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HPC07483.1"
                     /translation="MKETVFQKFKEDMEKTIAGLSKSFSRIRTGRASVSLLDGIKVDY
                     YGAPTPIAQVANVSVPESRLILIVPWDVSIIGAIEKAIQKSDLGLTPANDGKVVRLSI
                     PQLTEERRKEIAKNVKKMAEEGKVKLRNARRDANEELKKLKKDNKISEDELFTAQDEV
                     QKLIDQYIDKTDKILAAKEKEIMEI"
     gene            28489..29223
                     /locus_tag="PLF77_01120"
     CDS             28489..29223
                     /locus_tag="PLF77_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPC07484.1"
                     /translation="MVKIDVNKLPRHIAIIMDGNGRWAKQHAMGRIRGHKKGAEAVRT
                     TVRTCREIGVKYLTLFAFSIENWERPDKEVKALMYLLEEYLNAELDELQKQEIRLTTI
                     GETDRLNETVREKLIETIDRTAQNDKMVLNLALSYGAKDEILAAVKTVVRDAQTGSLD
                     IDRLDKETFSRYLYTAQMPDPDLLIRTSGEYRISNFLLWQMAYTELYFTDVLWPDFTR
                     EDLLKAIASYQKRERRFGLTSEQLQE"
     gene            29261..30079
                     /locus_tag="PLF77_01125"
     CDS             29261..30079
                     /locus_tag="PLF77_01125"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPC07485.1"
                     /translation="MAKTPSSLIKRWLTGIVLVPLLLLVIFFGSEAMFAAVVILFICG
                     GVWEYHHIVFGQGFVMEKIESLAFAVLIPLCVLSGNHQYLTALLALAVMLVFVLFIGS
                     IRESSFDFLSVAKVVFGIMYIPFLMSHFIAIRMLDQGVWWIMFVLVLAFAGDTVALYT
                     GKYFGKHKLVPLISPGKTVEGLIGLVVGSTVACLIYSHYLLPDVAVVHVAVMAFVGGM
                     IGQLGDICESAIKRGYGLKDAGSILPGHGGILDRLDCLMFIAPFVYYYRIFVIG"
     gene            30080..31243
                     /locus_tag="PLF77_01130"
     CDS             30080..31243
                     /locus_tag="PLF77_01130"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HPC07486.1"
                     /translation="MKKITLLGSTGSIGLSALDIIEKNPKRFRIAALAAGENIQLLKR
                     QIERFKPEMAAVKNKETAVKLRRALRPGTRIRISYDTEGLQETAACSSADIVISAISG
                     AAGLLPTLAAIEAGKDIALANKETMVMAGRIVTRAAAKKNVRILPVDSEHSAIFQCLE
                     GSRKEDLKRIILTASGGPFLNVAARQLKKVTLRQALRHPRWKMGKKITIDSASMMNKG
                     LEIIEARWLFNTSFDQIDVLVHPQSLVHSMVEFIDGACLAQLGVPDMKIPIAYALTYP
                     ERIANDLKILDLVQAGRLDFYKPDLKKFPCLKIAREAGVSGGTAPAVLNAADEVAVAA
                     LMQERLSFADLPKIIEKVLDAHDVIPDPSMDDILQADADARQQTEKIIGRITT"
     gene            31240..32331
                     /gene="rseP"
                     /locus_tag="PLF77_01135"
     CDS             31240..32331
                     /gene="rseP"
                     /locus_tag="PLF77_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPC07487.1"
                     /translation="MTTLISFIILLGILIFVHELGHFLVARMSGVGVQKFSLGFGPKI
                     IGRTIGETEYVLSWIPLGGYVKLMGESGTEELSPEDEKRSFARQNVWKRIMIVAAGPA
                     FNFLLAVVIFIAVMMYGLPTLTSDIGEVQKDSAAYQAGMLNGDKIISLDGKKIALWEE
                     IRDVVARAKDRQIEAVVQRGKETVRLQITPKLSKSKNLFGEDVSAYLIGVSPAGQTVI
                     ERKSPVDAVVSSVAKTWEISKLTVISVIKIFEGVISPKTLGGPIFIAQVAGAQVREGI
                     IPFVLFMAILSINLGVINLFPIPVLDGGHILFYLIEVVTRREVSAKVREMSQQIGFVV
                     LLMLMLFVIFIDIERLNIKFVNDVVKFFK"
     gene            32333..33016
                     /gene="tsaB"
                     /locus_tag="PLF77_01140"
     CDS             32333..33016
                     /gene="tsaB"
                     /locus_tag="PLF77_01140"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPC07488.1"
                     /translation="MRILAFDTSSQTASVAVLQDDDILYDVIIHTGLNHSETLLPAID
                     QACLRTKIPLPEFDVFACTLGPGSFTGLRIGAGTLKGFMLATGKPAVGVSSLAAIALN
                     AEKTSKKIYALMDAGRGQVYTACFQYGAGGMIEQQGGEQVANPSDIVYDADAVYVGSG
                     VIKYGAAFMPERQNGILSTAKQHCRASAVAVLGKAKYERGERLNAENFVPFYLRSADA
                     DQKKSFFGK"
     gene            complement(33030..33851)
                     /locus_tag="PLF77_01145"
     CDS             complement(33030..33851)
                     /locus_tag="PLF77_01145"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC07489.1"
                     /translation="MNIANSKNINKDNYSPSDYENWGFIYLENKEFDQALKCFQKTIE
                     LEPHNPKNYIHLGYVYEKMDMLDSAKKSYEKALEVKSDYIEAILKIGHILEMQGDYHN
                     AVELYTRAIDIAPQAIEPRFCLATLYDRHDMYDEAAEEYENIIEINPKHTKAIHNLGR
                     IYFQDGDYARALKSFQKVLNIEPENTDAWNDLGSVYEITNNLIQAISTYRKALKVNPL
                     HEDTNYNLANAYFSLFLSNPNIVKIDDIVERLQLVLSQNPNNRKVWELLSKIKAS"
     gene            complement(33929..35956)
                     /locus_tag="PLF77_01150"
     CDS             complement(33929..35956)
                     /locus_tag="PLF77_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC07490.1"
                     /translation="MYCDYWNLTKPPFDNVPDASMYVDCHESMENVISETLFAIKEGN
                     ECLTVIVGEVGLGKTLSLRVIIDSLEPEKYKVALITNPALSFVQLLREIIGQITGKQC
                     EVKNKVDLLEIFNRLLFETADQGRKIVIVIDEANVLSPSNLDDLRLLTNMQDDERNLF
                     TLLLVGQMEFAKTLEHPKRANLFQRIGTYGHLEKLPSPEAVRTYIESRLRIAGTDRKI
                     FDDDAIPVIWEFSEHGVPRLINKICKLCIKAGETNEFQTISGDVAQQIADRFQKLSKT
                     SAQKRKAPAAEAQPAAEAPRKQEPERIPKPSRPAFPEKETPAVSAPVRPAPPRMEEPA
                     PMRPRMAPPEQPPARPTAPPVIDEAPPVRPRPVMPEQPPMRRPLHPGMEDLMPPRSRM
                     APPEQPPVSPALPSMEEPAPMRPRMAAPEEMPLRPTAPPVMDEAPPVRPRPVMPEQPP
                     VSPAPPRMEEPAPMRPRMAAPEEMPLRPTAPPVMDEAPPVRPRPVMPEQPPVSPAPPR
                     MEEPAPMRPRMAAPEEMPAYTAPVRQEEPIPVRPQAVPVPPKPSASVVPPPVSAPGNV
                     APPQGVRAADTAQQSYEEQPMPMEAVEEEDFEEVTIGQQSIKLAIPSHVIRQAQSSNK
                     ENKNKLAGYWSAQILKGNPQLTHSSFADPVSLWFEVKNVILKKFNSPAHMQ"
     gene            complement(36026..36652)
                     /locus_tag="PLF77_01155"
     CDS             complement(36026..36652)
                     /locus_tag="PLF77_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPC07491.1"
                     /translation="MEKICIVKRRREYTQPEVKVTVTQPAISETAAPEVAVSRVDVKN
                     NAGLSENTARTTKESMTPELTIRSVGMEHEIKIPERKIVEEFVDNASSVIAITMTKEQ
                     SDLLLQSEYIKELLSGKKSDPALDIKVTPEGRLSLNYRFSDSLLLRMIAPNQVCQMLQ
                     VSRSFLQKLVHENKIKSYKLGRMRRFLLEDILEYLSSEVDFMQPSDDK"
     gene            complement(36972..38666)
                     /locus_tag="PLF77_01160"
     CDS             complement(36972..38666)
                     /locus_tag="PLF77_01160"
                     /inference="COORDINATES: protein motif:HMM:NF028259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07492.1"
                     /translation="MKTLLSLIGPRVRSSLNSLTRGNRKNVWSRLVMFGGLGLLFWAG
                     IFMIFYRVLLYFHSVQDFGDILAMKFVSMIILTFFMLLLFSNIINCLSHLYLSQDLPL
                     LHSLPVSANDIFFSRWIISAFDSSWMILAFSLPVFLSYGIIFHTGIFYYAIFTTVMIF
                     MCLIAAALSGILVLFGSKVLPAGRIRTILILLGALMMIVLIFVLRFTRPEQLVNPDSF
                     ASVVLYLNALKTPSSPVLPTTWITDAILAALKNDRATALLNAGLVVTFACMLVFISSM
                     LARNMYFPGFSKSQMTPRRLFAPLRYKGFSWETLLNFLPRESKAFAVKEIRTFFRDSS
                     QWPQLFLMTALIVIYIYNFSVLPLESYPIKTIYLQNVFSFLNIGLAAAVLAAVAARFV
                     FPAVSMESEAFWIVQSAPVSIGNFLWIKFFLYYIPLMMMAEGLVVASNILLNVSDFMM
                     LLSVLTVFFLVPAIVGLATGLGAVYADFKSENPANIVTGFGGLLFMILSFGLIALVII
                     LEAGPVYTIFMADVLGKNLTALKILWAVLSFALAIFLCLLATFYPIHLGRKRLTVK"
     gene            complement(38663..39397)
                     /locus_tag="PLF77_01165"
     CDS             complement(38663..39397)
                     /locus_tag="PLF77_01165"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC07493.1"
                     /translation="MITLSELTKDYGTTVAVDKLSLTVNAGEIYGFIGPNGAGKTTTI
                     RMMGGILAPTSGSIVIGGVDMARNPVEGKRMIGFVPDRPFLYEKLTGMEFLEFSANLY
                     NVRRGLFPEKAKRLLQQFALWTWADELIEAYSHGMKQRLIIAAALLHDPQILIIDEPM
                     VGLDPEAVHMVKDILKDLAARQTTIFVSTHTLSIAEDLCHRIGLIHKGTLLAEGSLKE
                     LKQIARLGEARLEEVFLTIIKENTFL"
     gene            complement(39394..40869)
                     /locus_tag="PLF77_01170"
     CDS             complement(39394..40869)
                     /locus_tag="PLF77_01170"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPC07494.1"
                     /translation="MTRPFKWPMAAGILVTIAFAVLLLIRLDFFQTQTPPVAGIKMVP
                     PQQPQDIWMTIFQNDRKIGFVHRMLTKNENGFAFNENVFMEISTMGMPQALNLATEGQ
                     LNADMTLSSFTFKLNSSLFQFHIRGRVADNKLILYTGAPNRQQKNEMPLQALPHISGN
                     IYDAAFHAGMDKGTTRGFSIFDPSSMSVRTINVTRNADEIIPIMGNRLLLKKYCADFM
                     GAKHCAWLDKDGQVLKETGMLGLSMEKTSRENALAEIDGSAGVDFTQIASIPSNVDIA
                     DPEKLRKLQIRISGITPPLQRQLHLDGDRQHYHDGVLTITREDPADKGPAGGWPADIA
                     VFLKPAALIQSDDPQIKALIGKIVRPEDSAFVKARKIINWVFLNIEKKPALSVPNALE
                     VLKNRSGDCNEHAVLTVALLRAAGIPARSEAGLVYLRGRFYYHAWCVLYINRWITADA
                     VFNQIPADVTHIRLLRGETDQQIRLIGVIGKTNLEVLSQTR"
     gene            41125..41769
                     /locus_tag="PLF77_01175"
     CDS             41125..41769
                     /locus_tag="PLF77_01175"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPC07495.1"
                     /translation="MKHDSIIAAEGYPFVVFSLIVTVFVAFLGGNWWLILFFALVTVF
                     ILCFFRNPERSFPEDEKVLISPADGKIIKIEEVDVNGTLSGKFKKISIFMNVFNAHVN
                     RAPYDGKIEAVHYHPGKFFSANLDKASEDNERNEILIRTPDNRTVWAVQIAGLIARRI
                     VCWVTPGMPIRKGERIGIICFGSRVEVYLPVDSAISVKLQDKVKAGQTVLGYLS"
     gene            41766..42548
                     /gene="pssA"
                     /locus_tag="PLF77_01180"
     CDS             41766..42548
                     /gene="pssA"
                     /locus_tag="PLF77_01180"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPC07496.1"
                     /translation="MNVENAARKIRMKKGIYLLPNLFTSASLFCGFYSAIASFKEHFV
                     TAAIAILVSVIFDGLDGRVARLTNTTSKFGAEYDSLADVISFGIAPALLAYSWSLSVY
                     GKWGWVVAFLFVLCGALRLARYNIQIGIIESKVFNGLPIPAAASVIATTVIFFDYVGA
                     EGKFHNIFIMVFVIMISLLMVSSVKYYSFKDMSLLVRKPFTIFFWSTVLLIIIVAEPE
                     IMFFVIILGYALSGPVWWLIKFGFGKPKAVPENKNNAANGKP"
     gene            42585..43142
                     /gene="rsmD"
                     /locus_tag="PLF77_01185"
     CDS             42585..43142
                     /gene="rsmD"
                     /locus_tag="PLF77_01185"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HPC07497.1"
                     /translation="MRITGGKARGKMLLFPSGSKERPTTDFLREALFNLTGSLNEKTF
                     LDLFAGSGSVGLEAASREARQIVLVENNQKLAGVIRKNIPNGLQDGRLTIMATDVAFA
                     LRDLFRKQCSFDIIFADPPYHRGLVAVTLELLGKYPVLAKGGILVMQHSVKEPIPETV
                     LGIFHRTDQRKYGENALTFFKMERE"
     gene            43142..43645
                     /gene="coaD"
                     /locus_tag="PLF77_01190"
     CDS             43142..43645
                     /gene="coaD"
                     /locus_tag="PLF77_01190"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPC07498.1"
                     /translation="MQEDFTKRIAIYPGSFDPITNGHVDIIKRGKRMFDEIIVLIAYN
                     PNKVYLFNIEERMEMIREIFGETPGIRVDCYSGLLVDYLKMSGANIILRGMRALSDFE
                     YEFQMALMNRRQTKHIETVFLMSGFKWFFTSSKLIKEVASLGGSVKGLVPDNVYQRLT
                     EKYNTRG"
     gene            43649..44842
                     /locus_tag="PLF77_01195"
     CDS             43649..44842
                     /locus_tag="PLF77_01195"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPC07499.1"
                     /translation="MRLSARVGRISPSETLAITAKVNALRAQGRDVIGFGAGEPDFDT
                     PDNIKDAAIKAIQSGFTKYTPVGGTDELKDAIINKLKRDNRLEYKRSQIVVSCGAKHT
                     LYNLAQIYFDAGDEVIIPAPYWVSYPDIVTLADGTPVIVKTEEKDAFKMKPEQLAASI
                     TARTRALILNSPSNPTGAAYSPDELKAIAQVLLDKDILIITDDIYEKIFYAGFPFANI
                     ANVEPKLKDRVVVVNGVSKTYAMTGWRIGYAAAAEEIASAISKIQSQNTSNPTSIAQK
                     AAVEALSCDQTMVGDMAAEFKKRRDYIVAALNEIPGISCFSPEGAFYVFPNVSGIYGR
                     SCGGKVINGSADFIDYLLDEANVAGVPGAAFGSDSHIRLSYATSLKNIEEGIRRIREA
                     VSKLT"
     gene            44859..46262
                     /locus_tag="PLF77_01200"
     CDS             44859..46262
                     /locus_tag="PLF77_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPC07500.1"
                     /translation="MGGIFGVVSSANCIKTLFYGTDYHSHLGTEHAGMAVLGPQGFYK
                     TVHSVSQVQFKSRFIDEYKKMDGFFGIGAIDDDTPQPLIFRSRFGVYAVAAVGLINNK
                     NQLVNELLKEGANFGERAGGDINNAEVVSCLINRGDNIVEGIASMRAVIEGSICAVVM
                     TSEGIYAARDKFGRFPLAIGKSKNPQDQSYVVATEASSFENLGYELTQFLGPGEIVLL
                     TTDGVKPVKPAGTEKKICSFLWIYTGYPSSSYEGISVEGARERCGAYLAKRDNIQADF
                     VSGVPDSGVGHALGYAMASGIPYRRALVKYTPGYGRSYTPPAQEIRDLVATMKLCAVR
                     EVINGNRIIICDDSIVRGTQLKNLTIKKLWDNGAKEIHVRPACPPLMFPCIYTSSTRT
                     TGELACRKAIRALEGKEIEHPSEYLDHHSEKHAKMIDWIRRDLNVTSLKYLAIDDMIA
                     AIGLPADQLCLHCWLGK"
     gene            46357..47451
                     /gene="ispG"
                     /locus_tag="PLF77_01205"
                     /gene_synonym="gcpE"
     CDS             46357..47451
                     /gene="ispG"
                     /locus_tag="PLF77_01205"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HPC07501.1"
                     /translation="MATVEHSSFIRRKKTKQIHLGGVAIGGDAPVIVQSMTNTDTRNV
                     EATVRQIKALEKAGCEIVRIALPDDKAAASVSAIKKRIRIPLIADIHFHDRLALEAIR
                     QGVDGIRINPGNIAPGKMGAIIRLARERRVVIRIGINSGSLQKDLLKKYGGATPEALV
                     ESAVRNLELFEDRGFDLIKLSLKSSDVPATIEAYRAIAARTDYPLHLGVTEAGSLIGA
                     AIKSSLGIGTLLYEGIGDTLRVSVTGDPVSEMGLAYGILRALGIRRVGVDIVSCPTCG
                     RCEIDLPSLTRRIEKALAGRKDYLKVALMGCVVNGPGEAADADIGLAGGKGQGLLFKK
                     GKAYKKVREKDFVNILLEEIDTIIEERKKD"
     gene            47451..49178
                     /locus_tag="PLF77_01210"
     CDS             47451..49178
                     /locus_tag="PLF77_01210"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HPC07502.1"
                     /translation="MRYSEMFLPTVREVPSDAEIISHQLMIRAGLIRKLTSGIYSYLP
                     LGYRVIRKLEQIIREEMNRAGAQEVHLPMVQPAEIWQESGRWTYYGKELLRLRDRHDH
                     EYCLGPTHEEVITGLVRNDVKTYRQLPLNLYQIQTKFRDEVRPRFGVMRCREFGMKDA
                     YSFDVDEEGAEKSYEKMFAAYNNIFRRCGLQFRPVEADSGSIGGKYSHEFMVMADSGE
                     DAMVFCEKCAYAANLEKAEVACPEKKIIFEDDWLPLEDVDTPDVRTIEEVSAFLKVTP
                     QNIVKTLIFNADGKPCAVLIRGDHEVNEVKVKNYLAAAELELASDDMIREVTGAPRGF
                     AGPVNIKAPILVDYSVIDMVNFITGGNRQDGHLKNVNVGRDFKVEAFTDLRIVNAGDP
                     CPRCGQPIKTARGIEVGHVFKLGTKYSKAMKAVYLDKDGQEKIMIMGCYGIGVGRTVA
                     AGIEQNHDDNGIIWPMPLAPYQVIISPVNINEITIRETAEYLYNAMITEGVEVLYDDR
                     DERAGVKFKDADLIGIPLRVTVGHKNLQDGNVELKIRKTGANELCPLQDIVGRVREII
                     RQELNPDIA"
     gene            49206..51359
                     /locus_tag="PLF77_01215"
     CDS             49206..51359
                     /locus_tag="PLF77_01215"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HPC07503.1"
                     /translation="MLRINDIIEKAQTYLPAEDLEMIEKAYIYSASVHQGQVRLSGEP
                     YLTHPMEVAGILTDMKMDAATIISGLLHDTIEDTLTTADQLEHEFGKDVSFLVGGLTK
                     LSRISFGSQEERQSENFRKMILAMSSDIRILMVRLADRIHNMRTLQYHPKEKRIYIAR
                     ETMDIYAPLANRLGINSMRMELEDLAFQYIAPDAYNDLTAKIVKSNEARNRYTEEVKN
                     IIQQEMEKYSVACKVEGRAKHLYSIYKKMHEQHINFDEVYDLIAFRIILDADKEKSCY
                     EVLSIVHALWKPVPGRFKDYIAMPKANNYQSLHTTVIGPYGERVEIQIRTRNMHEWAE
                     KGIAAHWRYKEGGSYKDNEEDQIRKLREVLEIQQDIKNPKEFMSDLKLALFPEEVYVF
                     TPHGDVKSFPKGATPIDFAYSIHTDVGNQCIGAKVNRSIVPLRYKLQNGDRVEIITQA
                     GHHPSKDWLKNVVTTRAITKIRNYINLEEKKNSIVLGRELLEKEFKRHNLKFSGIVKS
                     DDIKKIFSAFSVNQLDDLLFQVGLGRISAKRVAHYFIAGEEVEAAEPAREKIKKKKAA
                     SPSMGVSLIGIDDPDDIMVNFAKCCNPIPGDDISGYISRGRGVIVHTSNCPYIKRMDQ
                     ERIVDVQWSSREEHTYPVNIKVVCDDIKGILTEVSSIISSFDVNISYAEIETVNMIAT
                     CKFIIDISNLPQLNQIMSAIRQLKFVKAVEWLHHF"
     gene            51424..52113
                     /locus_tag="PLF77_01220"
     CDS             51424..52113
                     /locus_tag="PLF77_01220"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPC07504.1"
                     /translation="MFHTKKILVLITFAVLACFGWGAAELIAAEKKYIILIDPAHGGK
                     DRGLKLNDDVSEKDVTLAVALQIKKELAQEPNLQIILTRETDKTLDREARQKMIKTLN
                     PDMVLNLHVNGGFGKEASGYEIYYPSCGADDPASMRKTDKTKPQSQCQNNSLKMAKLI
                     SDNFNALFPRKARGLRMADLSVTEGVLVPAVAVEMGFATNAVDQKNLMSKKTQAEMGK
                     ALAVSIKAFSR"
     gene            52117..52848
                     /gene="rph"
                     /locus_tag="PLF77_01225"
     CDS             52117..52848
                     /gene="rph"
                     /locus_tag="PLF77_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HPC07505.1"
                     /translation="MTERKSGRRFDELRPIKLIKNYLNTAAGSVMVEFGNTKVLCAAS
                     IDEKTAPFLRNTGRGWVTAEYSMLPMSTQTRTARESVRGKIQGRTQEIQRMIGRSLRA
                     VTDLSAFGEKTIYLDCDVIQADGGTRTASVTGAFVALVGLFDQLKRDGVVQNIPVRDY
                     LSAVSVGIIDQQVMLDLEYEEDSRADVDMNFVMTGSGHFIEVQGTAEKIPFSREQMTQ
                     MTDVAERGIRQIIERQKELVGDLIG"
     gene            52845..53441
                     /locus_tag="PLF77_01230"
     CDS             52845..53441
                     /locus_tag="PLF77_01230"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:TIGR00042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HPC07506.1"
                     /translation="MKIVFASGNLGKVREIREMLSCINIELVSLKDYPDIPEIVEDGK
                     TFLENALKKARTVSERTAQAVLADDSGLQVDALGGEPGIYSARYAGEGASDDDNINLL
                     LEKLKEVPPEKRTASFCCVLVLYNTDGTYESFESRWPGRIIDDRRGDNGFGYDPVFYV
                     PELNKTAAELPPEIKNRISHRGQSFLKLKQSLGRKMKK"
     gene            53445..53521
                     /locus_tag="PLF77_01235"
     tRNA            53445..53521
                     /locus_tag="PLF77_01235"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53479..53481,aa:Pro,seq:tgg)
     gene            53771..54733
                     /locus_tag="PLF77_01240"
     CDS             53771..54733
                     /locus_tag="PLF77_01240"
                     /inference="COORDINATES: protein motif:HMM:NF026253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WG repeat-containing protein"
                     /protein_id="HPC07507.1"
                     /translation="MERFRKLCVIILSLSFIFSVAACDNFSKFKEKMLSEEIISFPVK
                     QGNSWGFIDTTGKFVIQPQFDGAGRFSDGFAAVKIGDRWGFIDKTGEYAINPQFDVAF
                     LFHDGLASVGLGGGKVKVGFINKQGKYVINPQFDDAGWFSEGLTAVGKIDGQKVKAKV
                     GFIDKSGKYIIKPRFEDAGWFSEGLAAVKIDGKYGYIDKTGKFIIEPQYDLAFWFSEG
                     MATVKNGNKYGYIDKTGKFIIEPQYDFAFWFSEGLASVKSKGKYGFIDKSGRYVIEPQ
                     FDIAFWYENGLAPVGIGRGKVKVGFINKQGKYAINPQFDGVNVF"
     gene            54860..55753
                     /locus_tag="PLF77_01245"
     CDS             54860..55753
                     /locus_tag="PLF77_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC07508.1"
                     /translation="MSGTLYPFKVNDSLWILGNGYFHLYLLKGAKQCALVETGISATA
                     DLVLSQLEALDVRPDYLVVTHPHSDHITGLDHLRGAFPGAVVMIAAGAASFVAHPKAA
                     PAIIAEDRHMFRALAARGLFSQTAVVTMPPSLSGCRILTDGEALDLGGMSVNFLSVKG
                     HSPGNLLAWIAGTKTLLVSDSLGNHYPGRGFFPTFFTGYNDYMATIDRLESFRPKRMG
                     LAHNGFFSTSGAIADIFCLSRKSAQDVRRYIEDCAMDDEDLARSLFGFYYTDELAVYS
                     PDNILNCCRLLVRRVRELHTA"
     gene            complement(55812..57149)
                     /locus_tag="PLF77_01250"
     CDS             complement(55812..57149)
                     /locus_tag="PLF77_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HPC07509.1"
                     /translation="MNPHVFREYDVRGVVDKDLNEDFVFHLGRAIGTYARAKRIRNMT
                     VGRDCRLSSESYHRFLSRGLNAAGIHVIDIGMCATPMLYFSIRHLRVGGGVMITGSHN
                     PPEFNGFKICIGHSTIFGPQIQDLRKIMESGRYAKGAGRCREKDIAKEYENYLFKRIK
                     IQRRIKVVVDGGNGVGGYFAVPLLKRFGCEVIPVHCEPDGTFPNHFPDPTVEENLTDL
                     IRLVRKNKADLGISFDGDADRLGVVNDKGEIIWGDKLLLLFARHILKNHPRAAIIGEV
                     KCSQVLFDDIEKHSGKAIMWKAGHSLIKAKMKEVKAVLGGEMSGHFFFADRYFGYDDG
                     IYAALRLLEILSRTGKKLSGLFADVPKTFATPEIRVDCTDSKKAKVVEGIKQHFKNTP
                     GIVDIDGIRIPFENGWALVRSSNTQPVIVLRFEAASVKTLNKIRGEVEALLHV"
     gene            complement(57174..59069)
                     /locus_tag="PLF77_01255"
     CDS             complement(57174..59069)
                     /locus_tag="PLF77_01255"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC07510.1"
                     /translation="MEPEIFEEYLAGFKKAYPKKFVSEDEIFSHIRRGDHIFVASGCG
                     EPQYLVKAMIRYVESHPKAFFDAEIMHVYSLGLAPYTEPKYKANFRHNSFFIGNSTRS
                     SINAGMADYTPISLSQVPSLLASGVVQIDVALIQTSMPDEHHYLSLGVSVDVVKAATE
                     KAKIVIAQINPNMPRIHGDGFIHIRDVDFIIPYDEPLLEVSSPKTGDVIITIGKNVAR
                     LVQDGDTIQVGWGSLPNAVMFCLSNKKNLGVHTELLSDGLVDLIKAGVINNSRKTIDH
                     GKTVASFCMGNKSTYAFLDNNPSISLRTLDYTNNPVMMSRIDNMVAINSALEIDLSGQ
                     ATSESIGGIFYSGIGGHQDFMRGALFARNGKSILALKSTSKNDTVSRIVPALKEQAGV
                     TLNRGDVRYVVTEYGIAYLHGKNIRERAMSLIAIAHPKFRPWLIDEAKKRGLIFKDQA
                     FIPGKRGEYPEDLETYLTTKTGLQVFIRPVKISDESLLKDFFYSLSDKSIERRFISTR
                     KDMPHERLQNFVIIDYTKQIVLVAIKGDVDNSYISAVGQYGIDDATHTAEVAFAVRDS
                     EQNNGIGFELLKYLTYLAKRQGLLGFTAEVLVENKPMLHVFEKGGFDIKKKIDSGVYE
                     LTLKFKL"
BASE COUNT        15817 a        14077 c        15464 g        14085 t
ORIGIN      
        1 attgagcgtt ctgacgacgg gaaaattatt ggattgagaa ccattgccgg tccgaattca
       61 accgaggaaa agtcgattaa tgatcaggaa gttattgatt ttcttaaaga caatctggat
      121 aacaacttcc ttaaaaatct tctggttcat tctgacgcag ggttgatcag gctgctggaa
      181 gatctcatcg ccctgctgac gaaaaagaac attattcttt ttacggaact gccggaaaaa
      241 gcccaggcaa agatcattga acgggaaaaa atacgtgaaa aaatatcatc ggacaatatt
      301 atcgtagaag atattcttta atgcagttag tatgttgtaa tttaaaataa ttgcttgaca
      361 aggattaaaa tagtaataag attttgccgc aattgaaatc gcaaggtgaa ggaatccaaa
      421 gcccatctaa atacagtttt aaaaatatga aattaaagct gtacttacct aaatgtggat
      481 ttttaaaaag taaacgccgg atattacgtt ggctttaaaa aatgcactca gtgttataaa
      541 aaaattgaat aaaaaaagaa taatattttc taaccgtaag catatatttt tatatctata
      601 aggaggattc tatgaaacct tatattaaga atgttttcat cttagttgtt atcttgcttt
      661 ttgccggaac gaccggcgtc aggggagaaa cacttcgcga ttcagtaaaa tcagtgcttc
      721 agaataatcc ggatgttact tctgtggcct ataatcgact ggcaagggac caggaggtcc
      781 ggcaggccat ggctgatttt ttccccagga tcgagaccac gttgtcggat ggctatattg
      841 accaacatca tccttatctt ctggatgatg aatttcatcc gagatcagcg accgtcagac
      901 tcaaccagaa tatattccgg ggaggtgcta ctttcgcgga aacaaaacgt caaaaatcca
      961 gagtgatttc tgcggcctat caattgcagg gggtttcgga gaatacggca ttgctggcgt
     1021 gcagagttta cttaaattat ctgcgggcca ttgagcagga taacctggcg aaagaaaatc
     1081 ttctgattca tgagcgattt tacgatcaaa tcaagttaag aagtcaggcg ggtgtcgacc
     1141 gtagagttga tttggatcaa attaccgccc gtttggccct ggccaaatcc aatctgattg
     1201 ttacccaggc aaacatcgag aatgccaaaa cggattatca ggccgtgatg ggatacctgc
     1261 cggttaattt ggtaaaaccg gaaccggcct acgctgaaat accggccacg ctgcaggatg
     1321 ctgaaagaat tgctctggcc agtcatccga cgttaaaatc ggccaaagcg gatattgaag
     1381 ccagaaatta tcaacacaaa acggctcggg cgcttgccta tcccaccgtc gatgtcggtg
     1441 cgggatatac ctggggagag gatttgaacc ggccggccgc ttcttatgac tatgaagatt
     1501 atttacagct caatgcgacg ctttctttca atatttttaa cggattcatt aatcaggcaa
     1561 ggatcaaaga aacatcgtat ctgattaacg aagcggaaga aatcgccaag aaaactgaac
     1621 ttcagaccat tcagtctgtc cggctgtcgt atgagtcgta tttggccgat cagcggcgcg
     1681 tcagggaact ggagcagtat gtcgattcta ccgataaagc ggcggaggct ttcatttctc
     1741 aatggtccat cggaagacgc agtctttttg atgttctcga ttcgtccgct gaaaaaatta
     1801 cggcaaaaag cgatttaatc aatgctcaat atgataaaat gtacagcgcg tacagaattc
     1861 tgagcggttt gggcaatctg gttcacaccc tggggctgca gtggccggaa gaaagtcggg
     1921 tggataccgg tgcgggcatt gttgatatga gctttcttga aaaataaccg gataatgtca
     1981 aacaatgcgc ttgattgaga acatcaggaa taggcggata ttcttttaat gatcttttga
     2041 tgacgaaaag attccgccca tgaccgtgca gaaatgaaat tgaatgtcgt gctcaagcgg
     2101 atatctgaac ccgataagag gtcgcgttca gaggtgttga tctttgcatg accggatccc
     2161 atggcttatg aaatactgga aaattacagg cgtgatcagc gcggcacttt gtcttcttat
     2221 tacccatcag gcatgggcag acagggaatg ggtctgcctg gaatccaatt cttcaagaga
     2281 atcattttat gacaaaaact ccattgtgaa agtggatgaa aatgtctttc gtttatggat
     2341 gaagcaggtc ttaagcgaaa acggaaaaat aaaaaccata tcgaaattcc gcaacacgaa
     2401 caaaccggat aacagccttg atttaataag ctatatttta aggcagatgg acgtcgactg
     2461 ccggaataat caatataaag acagcgccct tatcttttac gatgaaaaaa gcaacgttct
     2521 ttattcttcc ccgaagggtg cgatagggaa atgggatgat atcgttgaag ggtcgtttgc
     2581 gtaccagttg aaaaacgtaa tttgcaaagg accgaatctt gctgagaaac cctctgcgat
     2641 tctatccgcc gaccatgcga aaaagccggc tgaagcgggc actccgtcac aaagtttgcc
     2701 cgccggtact tccgatgaag acattcgtca ggtggtcagt aaatggctgg cggcctggca
     2761 gtcgggagac atcgcaacct atcgcagttg ctatgatgaa gattttaaat cgaaaaatat
     2821 gacgctggaa aagtggatcg catataaaac cggcgtgcgt caaaaaagca cggatataaa
     2881 gatcggcatt gacgatttaa aaattatcgc ggatggagat cacgcggagg cggtttttat
     2941 ccagacatac agctcttccc tattgaacga caagggtaaa aaaacgctga agcttaaaaa
     3001 agtcggcaat gaatggaaaa tatatcaaga gatcatgtga agccgcatgc acgcagccat
     3061 gagcctgcac cgaaggatca tgaccgtccc gtttttttct gtattgtcga tttccgtgtc
     3121 cgccccgaag tcctccgttg tgcgatccgt tcacgttata aatgtcctat atcggggatc
     3181 ccataattta ttcaggaaat tgtgaattac acaaactttt ttcctctgct tactgcttga
     3241 tttttccccg tgctctgata taaagcccca atcacaatgc atttgctgcc ctgatcaggc
     3301 tctcatctgc tattccatta gttcaggcgg caggaacagg aaggaagtat aaattaaatc
     3361 atggattatg agggatttat catcaggccg ccaagcgaag cttacagttt gcttttacag
     3421 gtgaccacgg gatgttcaca taataaatgt acgttttgcg gcacttaccg gcaaaagaaa
     3481 tttaaaatta aaccgattga acgcattaaa aaagatcttg acgaggccag acaatatgag
     3541 gacgtcgaca gagtgttttt atgcgatggc gatgcgctga tcatcccgca gccgaaactt
     3601 aaagaaatac ttgagctcat taacgaaaac ctgtccgata ttgagcggat cggaacctat
     3661 gccaatgcga aaagtatttt gagaaaaagc gttgaagaac tcaaagaatt aaaatcgctg
     3721 gggctgaaaa ttgtttatct gggtgttgaa accggcaatg cggaactatt gaaaaaaatt
     3781 cataaaggtg taacgtatga gcagatgatc caggccggcc gtcgcattaa agaagcgggc
     3841 attattcttt ctgtaacagt gcttttgggg ttggggggca gggaaaaaag tattgaccat
     3901 gctctggata cggcaaaaat tctgacggct atggatccgg actatgtcgg cgcgctgacg
     3961 ttgatgctga ttcccgaaac agaattgtat gaggattatc ttgcaggtcg ttttgttctt
     4021 cccgatcaat tcggttttat ccgggaactt tacctgatga tcgaaaaatc atcttttacg
     4081 gactgctatt tcacatccaa ccatgcgtcg aattatctgc cggtcaaagc gcacctgccg
     4141 cgtgaaaagg aaaaaaatct gaaaatgatc ggctctgtga ttcacgaaaa agacattagc
     4201 cggctcagac cggaatattt gcggggccta taactcaggg agaagatatt tgaacatcaa
     4261 aaaaaacatt tccaaacttt ttttctggcg cgaacaaaaa tatatcaaaa acaaaatcaa
     4321 tgccatgttg tccgtcgccc agaaaagcgg tttggtcgat gcgtcgtccc gggagatgat
     4381 cgagaatatc cttgagtttt cccacacact ggttcgcgaa atcatgattc cgcgcacgga
     4441 tattgtcgcg gttagcacgg aggcggcaat agatgacatc atccatgagg ttattgcctc
     4501 aagacatacg cggatccccg tgtaccggga taccattgat aatatcatcg gcatcctcaa
     4561 cgtcaaggac atcctcatgt tctggtccaa gccgatgacc aaggaagatt tgattgcctg
     4621 tttgagcacg cctcattata ttccggaaac aaaaaacacc cacctcctgt tttatgaatt
     4681 gaaggaaaat aaaaagcata tggctatcgt catcgacgaa tacggcggga cggcgggtct
     4741 ggttaccttg gaagacatgc tggaggaaat tgtcggagaa atccgcgacg aacatgatgg
     4801 caatgtcacc gatgaccgca tcattcagtc gccggacgga tctctcatat ttgacggccg
     4861 catggagatt gaaaaaatag aagaaaacct gaacatcgat ctgaaaaaag gcagatatga
     4921 aacattgggc ggtctgatcc ttgatgcgat aaagcgcatt ccggtttccg gagaaaaatt
     4981 ccagattgaa ggactggacg ttaccataga caaggccgat gaaagaagta tcaaaaaggt
     5041 caagataaaa aagattgaaa ccgacaacga cgatgaaaaa taattttccc gaagtctctt
     5101 tggcagacat ttcccggaat cttttccgga agaatatctt ttttgccgcg ttgagcggta
     5161 tcctgctgtt tttgtcgttt ccgaagtacg gatccggtgt attggcctgg gtggccctgg
     5221 tgccgctttt ttacgcgctg aaaaacgctg cgaccatccg tcatggactt cttctgggtt
     5281 ttattaccgg gatcatcagt tatgtgggga tcatttactg gattgcccac gttattgtaa
     5341 actacgggca tcttccgctt tatatcggaa ttgcgctgat gctgcttctg gccgcgtatc
     5401 tgagtcttta cctgtcgttt tttgccggat gtcttgtgtc ctttcgtgag aaagtaccgt
     5461 tttacatcat cgggccggct ttatgggttt ctctggaatt ttgcaaatcc tttattctga
     5521 caggttttcc gtgggagaat ttggggtatt cgcagtacgg aaacacctat ctgattcagg
     5581 ttgctgatat tttcggtgtt ccgggtttgt cctttctcat tgttttggta aatgccgttc
     5641 tcttggatgt gatccacagg agaacaaaaa aagaatatgt tgccgccggc atggtttttg
     5701 ttctgattgc cggaatctat gtgtacggat ttttccgtct gagagagatt gatgcgacgg
     5761 tgacaagcgc tccgggcatt gacgtgtcgc tgatacaggg caatattgac caggccgtca
     5821 aatgggatca ggattacaag caggagacat tggatatcta tgaacggcta tctttaaaaa
     5881 acaagcccgg ccggggcagt ctcataatct ggccggaaac ggcggttccg tttaaatttc
     5941 aaaacatcaa ccgttttcgc cgtcagatca ttgacctgtc tttaaaaact caaagctggc
     6001 ttctgttcgg ctcggtgagt tatgagcccg gagcatacgg caatgatttt tttaacagcg
     6061 cctatctgct gtctccctcc ggtgacgtca gaggacgcta tgataaagtc catctcgtac
     6121 cgtatggcga atatgttccg ttcagggatc tcatgccctt tgtcaaaggg ctgacgtcag
     6181 gtatcgggga tttcagttcc ggggcgggat ttgatcccct gtccctgaat gataaaaaaa
     6241 taggggttct gatctgttat gagggcattt taccgtttgc ctcgcgagcc tataaaaata
     6301 aagccgccga actgctggtc aatattacca atgacgcctg gttcggcgca acgtcggcgc
     6361 cctttcagca tttctccatg gcggttttca gagcggtgga aatgcgcctg tacctggtca
     6421 gagcggccaa taccggaatt tccggaatta ttgatcctgc cggaagaatt attgcaaaaa
     6481 cgggtatctt tcaggaagag gccttgaagg ggcatgcaaa atttgtcaca ataccgacgg
     6541 tgtatgaacg agtcggggat attttcatca tcgggtgttt tatgatggtg agcatctgtt
     6601 ttattttcag tttaagacga aggagtcaaa aatgaccacg gaaaacattc tggaaacgat
     6661 cagcgatctg aaaagaagaa ttcagcatat cggggagtgt ctttgacgtt ccggaattga
     6721 aaatgaaaat aaaagatctg gaggagttat ccttaaaaaa ggatttctgg aatgatcagg
     6781 attatgcccg gtccgttctc cagaagaaga caaatctgtc cgaaaaagtg gaaagatggg
     6841 acagattcag caaagacatc agcgacattg aaaaccttgc ggaaatggcc gtcagagaga
     6901 aagacgagca tgtcctgcag gatatcgatg aagagctgga acagttgaat gcggccgttg
     6961 cccaggaaga actgaagatg atgctcaacg gcgagcagga tgctatgaat gccatcgtat
     7021 ccattcatgc cggcgcaggg ggaacggagg cgcaggactg gacggaaatg ctgttgcgga
     7081 tgtatctgcg ttgggcggag aagaaaaaat ttgcggccag gatcatcgat tatctgccgg
     7141 gggatgaggc cggtgttaaa agcgtgtcgt ttacgcttga aggagagtat gcgtacggat
     7201 atgccaaggc ggaaatcggc attcatcgtc tggtccgtat ttcccccttt gatgccggtg
     7261 cgcgtcgtca tacatcgttt gcctccgtgt ttgtatatcc cgaagtcgat gacgatatca
     7321 aaattgaaat caatgacgat gatttgcgca tcgatacgtt tcgtgccgga ggcaaggggg
     7381 ggcagcatgt caataaaacc gattcggcgg taagaattac acacgtgccg acggggattg
     7441 ttgtacagtg ccaaaacgaa cgttctcaac atcagaacaa agcgatggcc atgaaatttt
     7501 tgaagtcgcg gctctatgag agggaactac agttaaaaaa tcagaaaatc gatgaggaaa
     7561 acaagcagaa aaaagacatc gcgtggggca gccagatacg gtcctatgtt ctgcatccct
     7621 acaaactggt caaggatcac cggaccaatg tcgaatgcgg caacgccggt aaagtgctgg
     7681 acggagacat tgatgaattc attcaagcct atctgatgat gacgacaacg acctcgtaat
     7741 cggatgggcc agtgcacggc aacttctcct ttgatcgtcg cgtttttgtc caagggaaat
     7801 ttaatttttg gaaaaatgaa aaattatgct ataaagatga accatacggt acggccatga
     7861 tggaaggata tgcagaagcg tgtcgtattt atgaataagg agttctgaat gaccggattg
     7921 acaagaaacc gtctgatagg cctgattgct ctcatctgtc ttttcatcgt ccctttaatc
     7981 ggatatgctg aaaattctat tgacaaaaaa agtccggagt cctccggcga catcacgaaa
     8041 caaaaaatag accagaaaat aaagctcgcc gcgttgaata acgacgacga aagcagcctg
     8101 tttgattttc cgcaggaaaa cgatcccttg gcggatgttc aggaggatga tgaaaaacag
     8161 gacatgatgg aaaatgcact ggagttattg ggtgaggccg acaaactttg gaaaaacgga
     8221 gatatcgaag aaaccctgga tacactggat gaggcttatt cgttaattct taatgccaac
     8281 ggcgattcgg caatcgctca ggaaaaagac gatctgcgtc ttttaatttc ccagcgcatt
     8341 ctcgctgttt attcctccaa acgggatgtg ctccagggga aaaacggcga gattcccttg
     8401 ttaatgaacg gcgatgtgca aaaggagatc acttcttttc agaccgtcga gcgcgacagt
     8461 tttcttgccg cctatgagcg gtccggcatg taccgttctg aaattgtgaa agagcttaaa
     8521 cggtccggga ttcctgaaga gttcttctgg ctgcctcttg ttgaaagcct ctttaaaatc
     8581 aatgcttatt cttcggcccg cgcactcggg ttatggcaat tcattccttc gaccgggtat
     8641 aagttcggac tgaatcgcga tgaatgggtt gatgaacgga tggatgtgca aaaatccacc
     8701 aaggcggcca tcgcttattt gaaagaactg catggtatgt tcggcgactg gcttacggcg
     8761 ctggctgcgt ataattgcgg ggagggcagg gtcctgaggg tgatttcacg acagcggctc
     8821 aattacctgg acagcttctg ggatttgtac aggcaattgc cgaatgaaac agcgcgttac
     8881 gttccccgat tcctggccac gctgcatatc gtcaacaatc cgcaaaaata cggatttgat
     8941 ttgagccata ccgccaggcc gattgaattt gaaacggtcc gggtaaacag gatcatgaag
     9001 tttaacgata ttgccgaaag aatcgacacg acggaagata tcttgtgcct gctaaacccc
     9061 gaattgaggc ataagatgac gccggatcgg gaatatgatt taaaagttcc gggaggcatg
     9121 ctggataaat tcagttttgt gtatcatgaa atcccgtcca gtgagaagcc gagcatcgcg
     9181 tccgtccaga cgtatgtcaa ggcatccgac cgaacacgca ggacctacat caagcatcgt
     9241 gtcaagagag gcgagaccct ttactccatt tcgcgaaagt ataaagtttc gatttccagc
     9301 attcgatcct tgaataaatt aagtccgaaa aagaaactga ttcaggggag aagactgctc
     9361 attcctgttg cgtcggccaa acggcatgca aagggattga ccgtaaaaaa aaaattcaaa
     9421 aagaaataac gtccataggt cgttataaag ttaaaaaagg tgaaaacctt tctacgatag
     9481 cctgccggtt ttcggtgtcc gtatcggaac tcaaagcgat caataacctg tcgtcgggac
     9541 aaatttacgc aggtcagcgc ttgaagattc ctcaaaagga agacggaaag gattccggca
     9601 atgaagattc cgaaaaaaat ctgagaacat ccgctcaaaa atcaaaaatc gataaaaaaa
     9661 tattgtcggc aaaggatatc aatcagctgg ggccggacaa atatattgtc acgaaaaacg
     9721 ataatctgca caccatcgcc aaaaaaaata ataccaatgc ggccagaatc atggagttga
     9781 atggcctgtc cgttgatgca aagattgttc cgggtcaaat tctggttgtt caataatcat
     9841 cgcggaacgt atgaatatat catgacttat tcggaaccgt atttattttc ttgacaaaaa
     9901 aagacgaata ctgataatga aaccctaaat tcgcttggat ccgaaaggac tgagacggaa
     9961 ggaaaatgca tcgaaaattt taaagccttt cgtcttgcga tgaaaggctt tttttattcg
    10021 aagatgaacg tcgtaaagtc agttcagaaa atgcagtctt tctctgaaaa cctgcgtttg
    10081 gagggaaaga aaattgcatg tgttcccacc atgggctact ttcatgaagg tcatcttgca
    10141 ttgatgagag aagcccgcaa gcaagcggac tgtgttgttg tcagtattta tgtgaatccg
    10201 acgcagttcg ggcctcagga agatttctcc aaatatcccc gtaattttga tcgagatatg
    10261 aagatggcgc aaaacgtagg cgtagatgtc atcttttacc cgtcgaaccg ggcaatgtat
    10321 cctccgcact atcagaccta tgtggcggtt gaaaaagtca cgcagaatct gtgcggagca
    10381 tcccggccgg gccattttcg cggtgtgacc accgtatgca ataagttgtt caatattatc
    10441 aagccgcatg ttgctgtctt cggcaaaaag gattttcaac aatttgtcgt cgtccagcgc
    10501 atggtgcgtg atctgaacat ggatctggag atcatcgggt atcccacggt cagggaagcg
    10561 gacggactgg ccatgagctc gcgcaacaaa tatctttccg ccgaacaaag aacctctgca
    10621 ttggttttat tcaagtctct ccaactggct caaaaactgt acgcgaaaaa agagacatcg
    10681 ccccgtatca ttatcgatgc cgtgaaaaaa atgattcaaa aaacacctct tacacaaatc
    10741 gattacgtga aaatctgtga cacgacgcgc ctgcaagata tcgcaaaaat cacagatcag
    10801 accgttattg ctctggctgt cagaatcggt tcgactcgac tgattgacaa tcatgttttc
    10861 ggtgaagcgc tgaaaatatc atattgaatt ttttaaggag acacttaaac catgcagaga
    10921 tttatgctga aatcaaaaat tcaccgggca acggtcacgg atgccgatct gcattacgaa
    10981 ggcagcattt ccattgatga aaaactcttg gaagcagcat ccattttgcc ttatgagaaa
    11041 gttgcgatat atgacgtgga caacggggag agattcacca cgtatgcaat caagggaaaa
    11101 cgaaattccg gaatcatctg cctcaacggc gctgcggccc gtaaagtggc taaaggggac
    11161 ctgatcatca ttgccagtta tgtggtggtc gaggaagcgg aaacgaaaaa ctggaaaccg
    11221 acctgtgtct ttgttgacgg taaaaacaag atcaagaaga aacattgacc gtatgaaggt
    11281 gtctgaccgg atcaatgtgc ccatggatat ccgccggata ttttttgtct cgaaaactga
    11341 agttcaatcc ccataaaaat attagactac attcatgacc tgtgttattg atgtgtatat
    11401 ttgacgcaac atcatgcaga ctaacgtgct gcttaaaaaa atgaaatcaa acttggagga
    11461 aacatggctt ttttggaaat cgatcctgct ttaaaatata aagtggcgga catagaactg
    11521 gcggactggg gaagaaagga aatcaaactg gcggaaaatg agatgcccgg tctgatggcg
    11581 ttgcgaaaaa aatacggaca gaagaaaccg ctcaagggca tgaaaatcat gggcagtctc
    11641 cacatgacca ttcaaaccgc catgctcatt gaaaccctca agacattggg tgcggaaatc
    11701 cgctgggcgt cctgcaatat cttttcgacg caggatcacg ccgcggcggc aatcgccaaa
    11761 aaaggatcgg cggccgtttt tgcctggaaa ggcgaatccc ttgaagagta ctggtggtgt
    11821 accgagcagg ctctgacctg gccggacgga tcgggcccgc atctgattgt cgacgacggc
    11881 ggcgatgcga ccctctttgt tcaccagggt gtgaaagttg aacgagatcc cgcgttgttg
    11941 aaacaaaaga tcgacaataa agaatttaaa atcatcttgg accgtctgga aaaatcattc
    12001 cggcgcaatc cgaaaaaatg gcagaagatc gcctcaacca tcaaaggcgt atccgaagaa
    12061 acgacaacgg gcgttcaccg cctttatcag atgcaggcgg cgggagaact tttattcccg
    12121 gccatcaacg tcaatgattc ggtgaccaag tccaaattcg acaaccttta cggttgccgc
    12181 gaatcgctgg ccgacgggat taaacgaggc acagacatca tgatatccgg aaagattgtt
    12241 gtcgtctgcg gttatggcga tgtcggcaag gggtgtgcgc agtccatgag aggattcggc
    12301 gcccgggtca tcattacgga aatcgatccg atctgcgcct tgcaggcggc gatggaagga
    12361 tacgaggtcc ggaccatgga ggaagtggcg ccgatcggcg acatttttgt ttctgcaacc
    12421 ggatgctgcg atgtcattac aggccggcac atggaaatga tgaaaaacga ggcggtggta
    12481 tgcaacatcg gccatttcga cagcgaagtg gacatgcatt atctggaaac aagcaaagtc
    12541 tgcagaaaag aaaccatcaa gccgcaggtg gacaagtgga cgttgaaatc gggacgctcc
    12601 atcatcgtac tggccgaagg tcgtctggtc aatctcggct gtgcaaccgg tcaccccagt
    12661 tttgtgatga gcaacagctt taccaatcaa tgcctggccc agattgagct tgccactaaa
    12721 aaacataaaa taggcgtata tacgctaccc aaggtgctgg atgaggaggt cgcccgtctg
    12781 catctggaac gactggatgc ccgattatcc aaactgacaa aaaagcaggc cgattatctg
    12841 ggcattaccg tggacggccc ttttaaaccg gatcattaca gatattaaat tccgggatgc
    12901 tttaagaata aacgcgttgc gctcaggtat tgactttgta taaaagaaat gcggcggcag
    12961 caccgaaaag aatgtttgcc gcccaggcgg ccaacacaac aggcagcatt tctgatttgc
    13021 ccagagacat gcagaaggca tggacgatcc agtaggaaaa accgataaaa atgcctatgc
    13081 cgatgctctg catgacgccg ccgcttcgtt cggagcgcag agcaaagggt acggcaatca
    13141 gaaccataat caaaatcaca aaaggaaagg ccgtcttgcc gtgcagatcg acgttgtatt
    13201 tgcgggcatc gaaaccttcc gattgtatct tttgcacata tcggcgcagt tcaaaatacc
    13261 ccattttttc aacatccttc tgcattactt tgaaatcttc cggtttttcc ggaagcggga
    13321 tgattttttc ttttgagcgt tccagaaccg gggcacggtt atcatcgaag cgcgtcgtaa
    13381 gcagatcatg aaaaacccag cggccgtttt tccattcggc gcggcgggcg tcaatgcggc
    13441 tgatcagggt aaagtccttg tttaaataag tgatcatgac gccgtgcagg atgttttttc
    13501 cgatgtcaaa cattttgaaa ttatagatgg cattgtcgct gcggtaccag atttcattct
    13561 gtttaaagaa gccgggcgtt ttttgttttt tcacctcgat gtttttgatg taggtcgttt
    13621 tttgaacggc ggccggtgta atcagttccg taaaataaaa gtaaaacacc gcggtgaaag
    13681 aggcgaacat gacgatgggc agagccattc tgaagatatt gatcccgttg gctttcatcg
    13741 cggtgatttc gctgaatttc gtcagggagc tgaaggtcat caatgtggcc agcaggatcg
    13801 agggaggcaa cacataggat ataatcatgg gaacggagaa gataaagtac gacgccattt
    13861 gtgccgccgt ggcctgatgg ctcagaaaca ttctgatgcg ttcgaaaaaa tcgacgatca
    13921 aaaaaagcgc aatgaacgcc agaaacgtta tcgtgaagaa ctttaaaaat tctttcagaa
    13981 tatatttatc cagaagtttc acgtcgacgt cctcggatga gtctccagtg tgccgggagc
    14041 aataaaaata tgtttaaaaa agataaatct ctttgctggt tatgtaaaac atgaaaatgc
    14101 cgatgaaagc aaatgaaata ttgggaatcc agacgccaac ggccacgggg atgtagcctg
    14161 tttcagccag cgcttcgccg ccgatgcgca gcagataata caagcaaacc gtgatcaggc
    14221 cgacggcaaa tcctctggcc ttgacggagc gctggcttcg catgccgagc ggcagcgcca
    14281 gcagaccgaa aaagatgcac gccagcggta tggaaaattt cttgtgggct tcgatggaca
    14341 attcccgaac atctttttcg ctcatcccgg gttttttcat gttttgcagt aattcggtca
    14401 tggtcatgtc tttggcggct ttcgacgctt tatcgaattc agccagggcg gcatacaaat
    14461 ccagattgat ttcatagctc tgaaaatcaa ttttacggta attttttaaa tcggaactga
    14521 ccgtgtggat agatccgttt tccatccgaa gcttcaccct catggtgtcg gggtcggaaa
    14581 ccaggaaggc ccggggtgcg ataatggtgt tctgttcgcc gatggtgcgt ttgtcggaaa
    14641 cgatgacctc ttccaaagcg tgattcccct cggaaatttt attggtataa ataataaagc
    14701 ccttaaagct gtcattaaaa actttttcct tgatgccgac actggcattt tgctgtgcga
    14761 tatcgaaaag cagccgcttg gtcgcgaaat tgctctgtgg tgcgacgaaa taactgacca
    14821 gcagcgtgaa aacaaacgcg agcagggaag cgaccgcaac cggataatat attctggcca
    14881 tactgacgcc ggatgtcttc agcgccgtga tttcattgtc ggaggataat cggccgatcg
    14941 cgatgaggat ggagatcaga agcgcaatcg gaatggtaaa aagcatgaaa tagggcagca
    15001 taaaaatgaa aatttttgcg atggaaaaaa tgctgacgcc tttattgatg aacaagtcca
    15061 tgatttgaag aattttcccc atcagtaaaa cgaacgtcag gacgcaaaga atcagaataa
    15121 aagggaacgc gatttcctta aatatatagc ggtcgatgat tttgcgcatg gcctgatcaa
    15181 ctcttttttt aatggatttc cagacataaa aagagcgccg tcgcgatcaa aaggatcccg
    15241 ggtcatgccc tgttttttgt ccgggttgaa attaacacgc aatgatgaat tttctcaagt
    15301 aaaacatgca gagattctga aaccatgttt gcatgattca ggcggcggac gttcgtgaaa
    15361 tgaccgacgg atttcgaaaa aaaacgattg gccttccatt cattgattga cagtttcaat
    15421 catttttttt atggtgatgc cgaatcttac ccgttgtgaa agggatcggt ctatgaaggt
    15481 tgatacaatg cgccggatag atttttatgc aggcgtcccg ctgtgttttt tcgggtccct
    15541 gttttataaa gcggtgaaat ggctttcccc gcggacaaag aaagatccga aaaatgtcct
    15601 gtttatagaa ctgtcggaaa tgggcagcgc cattcttgtg gatccggcaa tgagaaaact
    15661 gcgcgacaca accggcgccc agttgcattt tgccatcttt aaaaaaaaca agcccagcct
    15721 cgatctactg caaaccgttt cgccggacaa tatttacacc atccgggagg accgtctttc
    15781 gtcttttctg atggacacgt ttaaattcct gcattggacg agaaagagaa aaatcgacac
    15841 ggtcatcgat ttggaactgt tttcccgttt taccgcactg ctgaccggtt tcagcggtgc
    15901 ttcaaacaag gcaggttttc atgcctattt caatgaaggt ctttatcgcg gcgattttct
    15961 gtcgcataaa gtcgcctata acccgcacat gcatatcgcg aaaaatttca tcgctttagt
    16021 caatgccctg ctttcgccga aagaagaaat cccctattcc aaaaccagga ttcaggatgc
    16081 agaaatcagc atggccaaag tgcacatcac ggatacccag aaaaagacgg tcctggacat
    16141 cattcgcgga tgctttgatg ctttcgatcc ttcccggcat aaaattgttc taatcaatcc
    16201 caatgccagt gaaatgcttg ttcagcggcg ctggccgcag gcctattacg gacacctgat
    16261 aaaaatgatt ctgggacaat gttcccatgc ggttgttctg attaccggcg atccgcgtga
    16321 gcgggaggag gcagagcgcc tcaaggaaca ggttcaggat gagcgatgtg ttaacttcgc
    16381 cggcaaagta tcctttgccc agcttcccca actatacagc gtctcggaat ttatggtgac
    16441 caacgattcc ggaccgccgc attttgccgc cattaccgag atgcccacct acgtcatctt
    16501 tgggccggaa acgcctgcac tttacgggtc cctgggccgg accacgccga tttatgccgg
    16561 actggcctgc tcgccgtgtg tcagtgcatc gaaccatcga aagacggcct gcacagacaa
    16621 tgtctgtctg aaggtgatta cctcatcgca ggtttttgaa acgatcaggc ctgtgctcga
    16681 cagaatcaat gctgaaactc ttctgtaaag aaagcgcggt cagctttttt taaacagcgc
    16741 ttccagtttg tctccggggg tgttttgaga gggtgcatcg gatagcgttt ccgtttgccg
    16801 gaatctgaag aaatcgcaga aattggagcg ggctttatcc aggacgcgtt ccgcttgcgg
    16861 ttcgcggcag tcattatacg cgccggcatc gtaaaaagag cagttcaggc agcaatgcaa
    16921 atccgcgctg cagacggggc actgggattg tcgtccggta taaacattat ccgaaatttc
    16981 attgaggcat ttgtgacatc gtcgcatcat gtcaaaaaaa tacatgttgt gttgaatttt
    17041 tgtcaagcca atttgaaagc aacaatgcat ttccgagcgc gacggaaagc agggcttctt
    17101 gatttcatta tgttttaagc caatttctgg ccaggtcaag gttgctgaaa tatctcgctt
    17161 ccaaatgggt gttcagggtg gctgttttta aaaaagtttc caatctgtca ttgccgggaa
    17221 catccaggat agccgtcttt cccgttgcct tatcgggacg tctgatataa taaagaacat
    17281 cgtggtatcc cgagccttca ttaagggttc gcacgtcgat cagcaaccga ctgccttttt
    17341 cattcctcag ggaatcaatc tccttttgca accgcgaaac attggttctt gttaccgtgc
    17401 ctgaaagaat aatttcaatg attccgtcgt ttatcgatgc tgacaggcgg tattccttat
    17461 cgtttgtcac tttttcccct cacgatcgcg atacgctatt ctcgtccgcc ggacgatgat
    17521 gaattttgta aaagcggtgt attcatctct caataatgaa aaatataatt ttgcatatac
    17581 tcagattgta aaaaaatatc aatccctcga aagtgggatt ttgacgtgtc tgaattcgcc
    17641 atgcaacgag gaggcccggg gcgggagaaa actcttcaga gggcttgatc agcgtatttt
    17701 caatccggcg caaccggatt tttcttgtcg aggatgcgtg ccaccagcat acccgaaaca
    17761 ttgtcgccga cagcattaag cattgttgcc ggtgcgtcaa caagcgttcc aatcatcgta
    17821 atgatcggaa aagcttccat cgggaaacca taaaaggaaa taatcagcag ttcgccgatg
    17881 gtaccgccgc cgggaatgcc gctgaccacc acgccggtca gcaaagcaat cccaaaagcc
    17941 gttaaaaggg tttcggcacc tgaaaaaggc atattgaaga ggccgaaaag aaacgcaatc
    18001 ttcaaaacgg cggccaggca cgagccttcc atatgcatgg ttgcgccgac ggggataacg
    18061 atctcgcgaa tatcccgggg aacgccattt ttatcggcgg cttccagatt ggcgggaatg
    18121 gcggccatgg aactgccggt ggccagggca atcagagatg gcggaatgat gtttttccag
    18181 aagcgacgca tgccatcgat accggcggcg ataaaagcgt acaggctgaa agcgatgaaa
    18241 aaatacaaaa aagccgtggg gtaataaacc atcatggccc gtgcgtaaga gtcaagcagt
    18301 tgcggcccga aaacgccgac caaataagcg aaataggcac agaggccgac gggagcataa
    18361 tacatgataa aggaaatcac tttcatcatc acggcgcttc ccgaattcag aaaattcgca
    18421 aacggttttc cgcgctcacc cgtcacagag gcggccaatc cggttaatac tgaaaaaaca
    18481 atcagggcca gcatgttctt tttagaaaga agctctgtga aatcgccgac ggtgaaagcc
    18541 ttgacgatct ggtcggaagc tttgaggtgt tcgacatcaa caccgttacc cagaccgatg
    18601 ttcaagcctg cggccggcgg gaaaaagatc acggcggcaa tcatgaccgc cgacgcgatc
    18661 aggccggttg cgataaaaac aaaaatcatc gcagacatga tttttcccag ccgtcggata
    18721 ttttgcatac cggcgacggc ggaggctatg gaaaagaaga ccagcggaac gattgcggta
    18781 aagatcagat tcaggaagat atcgcccagg ggtttgaggc gtacggcatc ggccttaaaa
    18841 acaaagccca aaacggcgcc gatgacaatc gaagcaatta gtaaaatgga aaaaccgtac
    18901 gattttaaaa aagatggttg tgtcggattc atgttttcac tcgataatga aatcaaaata
    18961 tgtgtcgggg taaggttcgt ttttcagggt gtagtgccac cattccagat cgtaattctt
    19021 gaagccgtgc ttttccatca gcgatttgag cagcagccgg ttgatctttt gttgttctcc
    19081 gatttttgcg tttgccgtat gcgacagttc atgaaaacag tcaaatccgg tgcccatatc
    19141 gatgctgtta tccgcaaagc gttttcccga aggcaggtag cattcggata aggtttgtcc
    19201 cggggtgtaa tcgggctgcg gcggcgcgga caacaaaacg atagtcagat caacggtgct
    19261 gccgcggctg tggctggacc ggtcggcaat aaacccgtcc gtaaacagat tccgtttgtc
    19321 caccgtagga tagaattctt ttctggtttt cgtgttttcg atgtcctttg cccagcggac
    19381 gaaatgatgc accgcccgtt gcgggcgata acagtcgtag attttcaggg tcagcgaata
    19441 agcctcaaga tccttctgga ccagcgccag cgcatcggcc gcttctttcg tcagataaca
    19501 tttcggggcc agatagccgt caacccgttc gcccaggaag ttgtgcgcac cgtgataacg
    19561 gatatcaagc agcgcgcctg gaatgactgt ttgaatatcc acaaaacggt ccggcttttt
    19621 gttcaggtcg gagcaatgcg ccgcggacgc aaaataaaaa aaagaaaatg aaactgttac
    19681 tgccagtaaa aacctgattt tcatgttatc ctgctcccgg attattattt caggcccatg
    19741 atgatcagcg gcgctgccgc cggatcaagc cagtgaagaa gcctgaggat attttcttcg
    19801 gatactgcca cgcaacctgc ggtgggaacg ctcttgtcct tccaaacatg gacgaaaata
    19861 gcgctgcctc ttccctggac gaccgggttg gtgttatacg cgatcacgat tccgtattta
    19921 taaaggtcgt catcacgttt cattctttca tacgatgtcg attgcgtcac ggattgcttt
    19981 gtccagcggt tataatctgc cgcttcggaa tcgtcaaccc agatatcatc ttctttgatt
    20041 tggcgataga gcattttcgt gtttgcagag tcagcataac caaacgccat gaccaggggg
    20101 aaaatgccgg aaggggtttt tccgtcgcct tctctttttt cccctggcgg cgcaaaaccg
    20161 tttcgtccaa tgacggcgtc catggggggg gagaccgccg accagtttcc ttcacgttgc
    20221 tccatgacat aaacgattgt tcgggtgaaa atccagaaac cgcagcgttg cacaagaagc
    20281 acctgggaag aaccgtccgg ttgatgaggc ctggacatta aaaaatccgc agcatcaaat
    20341 tttgtgttga aacaggaaaa ggcgcatccg gaaacggatg aaaacgccat aattactgaa
    20401 aatataagga caaattttaa cggataaaat atcttcataa aatcattcaa tattttggaa
    20461 atgacggcct agaatacggt aaaactataa aaaaatcaag taataacagc agacgaagtt
    20521 atgcttgact ttaaatattt ttctgtgata gcaaagtgca tctgaatggc acttgatcga
    20581 taatctcata ataaatttcg caggaaagaa gcagtgtccg ataatttttt tacatttatg
    20641 attattccca accggaagag cggggtcaaa aaaatatccg ttccgaaggt tgccatccgt
    20701 aatattctca ttggcgccat cgtcgttgtt ctgatcgctc tgtttgtcat ttatgattat
    20761 gccagcatca agcgcgaccg ggcggaactg gcccggttga gagcgcagac gaaagaacaa
    20821 tcccgtcaat tgcaggatat gggcatgaaa atagatcagt ttgccgatcg cctggaagcc
    20881 ttgaggcagt atgacaaaca gatccgggtt ctggcccatg aaaccaacct gagcaagaaa
    20941 atgcccctgg gcgtgggcgg atcagaagaa gtcagaatga aggatttggt tgataaagac
    21001 catgaacaaa ttatcgcggg catgcgcaga aacttcgaaa agcttcatga agaagccaat
    21061 gaaagagaga gaagcttcac cgaactattg gggtatttgg gccaacagaa agccgttctg
    21121 gcttcaaccc cttcgctctg gccggtgaag ggatgggtga cgtccgaatt tggcatgaga
    21181 agatcgccct tccgatcggg aatggaattt cataaaggcc tcgacatttc cacccggttc
    21241 ggaaaagaag tggtcgcgcc ggcggacggt ttggtgatca gcagcggtta cggaccgcaa
    21301 gacggcaatt atatcaagat cgatcacggc catgggttta ctacagccta tttgcatttg
    21361 tccagaatga cggtgaaaca gggaacgcgg gtcaaccgag gagatgttat cggttatgtc
    21421 ggcgatacgg gacgcagtac cggtccgcat ttgcactatg ctgttttagt taacaatgtc
    21481 gaggtaaatc cgagaaaata tctgagataa tgattgactg attttacctg aagttgtatt
    21541 gatacattca ctgattgaat agaccggtta attggccggt ttttttttaa aaaaatatgg
    21601 aattttaggg tattgattga atgctggaca atatattgaa aaaaatattc ggcacaaaaa
    21661 acgaccggga attgaaacgg ttgtcgttgc ttttaagaga gatcaacaac ctggaaccgg
    21721 ccatgacggc tttaaacgat gaggaattaa aagccaaaac cccttatttc agggaaaaac
    21781 tcaaaaacgg attttccctg gacgatattc tgacagaggc ctttgccacg gcgcgggaag
    21841 cctcgcgccg gacgctgggg atgcggccgt ttgatgtgca gattatcgga ggcatcgctt
    21901 tacacgaagg caaaattgcg gaaatgaaaa ccggtgaagg caaaacgctt gccgccacca
    21961 tgccgttata cctcaatgcc ctcgaaggaa agggctgtca tgtggtgacc gttaacgatt
    22021 atctggcgcg ccgcgatgcg gaatggatgg ggccgattta taaatttctg ggtctgaccg
    22081 tcggcgtcat cattcacggg ctggatgacc cggaacgcag agaagcctat cactgcgata
    22141 ttacctacgg gaccaataat gaattcggtt ttgactatct gcgcgacaac atgaaattca
    22201 cgctggacga ttatgtgcaa cgcgaattca attatgccat cgtcgacgaa gtggacagta
    22261 ttctgatcga cgaagcccgg accccgctga ttatttccgg tccgtccgag gaatcaacgg
    22321 acaaatatta taaaatcaac cagattatcc cccggttaaa aaaggacaaa gattactcta
    22381 ttgaggaaaa gagccgtacg gtggttctga ctgaagacgg ggtcgcacat gtggaaaaat
    22441 tactcaatgt gcagaatctt tacgagccgc gcaacataga aatcgtgcat catgtgaatc
    22501 aggcgctgcg cgcccatacc ctgtttaagc gggatgtgga ttatctggtc aaggacggtc
    22561 aggtggtgat cgtcgacgaa tttaccggaa gagtcatgcc cggccgtcgc tacagcgacg
    22621 gattgcatca ggcgctggag gccaaagaaa acgtaaaaat cgaacgggaa aatcagacgc
    22681 tggcgtcgat cacctttcag aactatttcc gcatgtataa aaaactggcc ggcatgaccg
    22741 gaaccgccga cacggaagcg gccgagttca agaaaattta caacctcgac gttctggtga
    22801 ttcctaccaa catgccgatg attcgcgagg accacaacga tctgatctac aaaacggaac
    22861 gggaaaaaat caacgcggtc attggggaag ttaagacgtt gaacaaggaa aggcggccgg
    22921 ttttgatcgg aacgatttcc atcgaaaaat ccgagatgct gagcaaacat ttactgcgcg
    22981 ccgggatcaa acatcatgtg ctgaacgcta aaaatcacga gaaagaagcc gagatcgttg
    23041 cgcaggcggg ccaggcgggc atggttacca tatccaccaa catggccggt cgcgggacgg
    23101 acatcaaact gggcgagggc gtggcggagt tgggcggtct gcatattctg ggcaccgaaa
    23161 gacacgaaag ccggcgcatc gataatcagt tgaggggccg ctccgggcgc cagggagaca
    23221 gaggttcgtc gcggttttac ctttcgctgg aggatgattt attgcgcatc ttcggcgcag
    23281 agcgtatttc cggaatcatg gacaaaatcg gcattgaaga ggatcagccg atcgaacaca
    23341 aatacatctc ccgcgccata gaaaatgccc agaaacgcgt ggaagggcag aatttcgata
    23401 ttcggaaaca tctcctggat tacgacgacg tgatgaaccg tcagcgaaag gttatctatg
    23461 aacagcgcaa gaaagtgctg cgcggcgaca acctctgggc ggacacggag gagatgatcg
    23521 aggaaatcat cgacaatatt ttgtccgagt ttgtcgacga aaagacaaag aaggaagaca
    23581 taaatttcaa gggtctggac gatgccctgc tcaaacaatt caacataaga tttgatctgg
    23641 cgaataccga tcagacgggt gacggtcaat cagccattcg ggaatggatc attcagggcg
    23701 tgcagaaata tctgcgggac aaggaaaaag aattcggccc ggagctcatg aactacctga
    23761 tgaaagtcat catgcttcag gccatcgatg tccagtggaa ggaacacctg ctggccatgg
    23821 atcatttaag agaaggaatc ggtttgcgcg gatatgccca gaaggatccg gtccgggaat
    23881 atcagcgcga agggtacgac atgttcatgg aaatgatcgc ccggataaaa atggacgttc
    23941 tggaaaaact gtgtctgatc cggattcagc gagaggaaga agtcgaggcc atccggcaga
    24001 aacaaaaaca ggattacgtg atgagcagag gggaggatac gccggagaaa aaaacggtca
    24061 agcatgaagg cccgaaaacc ggaagaaacg atccctgccc gtgcggaagc ggcaagaaat
    24121 ataaaaaatg ctgcggagca tccgtttaac cgagaggagc taatttcatg atcaagaaca
    24181 aattaaaaat tagcgtaccg ggatttctgg ccggcggtat ttccgttggc attaaaaaaa
    24241 aggatcagaa ggatctgggc ctgatctact ccaccgttcc cgcccgggcg gcggcgatgt
    24301 ttacaaaaaa cacctttaag gcggcgccgc ttttgatcaa tgaagaccgg gtgaaaagag
    24361 gcatcgccca ggcgatcatc accaacagcg gttgcgccaa cgccgcgacg ggcaaagagg
    24421 gaatccgcga cgctctgatt gtttccggtg cattgtcccg ggaactggcc atcagagaag
    24481 aactgatcct gctggggtcg acgggcgtga tcggcaaaag actgccggtg aaaaaaatcg
    24541 aaggcggcat cggcaaactg gttaaagggc tcagggaaac gggaattgaa gatgctgaaa
    24601 aagccatgat gaccaccgac aaatatccca agatcgccat ccggaaagga gtcatcggcg
    24661 caaaagatat caccatttgc ggcatcgcca aaggcgccgg tatgattgag ccgaacatgg
    24721 ccaccctgct gacgtatgtc atgacggacg ccctgattga ttaccgggcg ttgaatacgg
    24781 tatttcatca ggcgatcaat gccacgttta attccatcag cgttgacggc tgcatgagca
    24841 ccaacgacat ggcgattatt ctggccaacg gtctggccgg gaataatccc ctggaaaagg
    24901 ttcaggcgcg tctgcagtta ttccgggaca tgctcacaga ggtgttgatg gaactctgcc
    24961 aggcggtcgt aaaggatggg gaaggcgcga caaaattcat ttccatcatg gtcaaaggcg
    25021 ccagatcgaa atccgacgcg cgctgcgctg cttatgcgat ttccaattcg aatctggtta
    25081 aaacagcctg tttcggggag gatcccaact ggggaagaat cattgccgcc ctcggcgctt
    25141 cgggcatatc catggacaag gagaaagtcg tcctgaccat cgccgatctc gcggtttttt
    25201 cgcaaaacac gccccaggag gtgctgatga gcagactgaa ggacgccttt aagaaagatc
    25261 gcatcgatat tgaagtggat ctgggagcag gcgacaaatc ctattatgtc tacacatccg
    25321 atctttcgca cgaatatgtc aaaatcaacg cggaatattc aacctgagtt tatcgaggcg
    25381 tcaaaatatt tattttccga taaaagttat taaaacttga ttatttgtgc ggtttgtgtt
    25441 aattgccgcc ggataaattt cacacatccc cggattgccg gtttgttgct tgaaggtcaa
    25501 gttggccggc caaggtgaag gggatggagg aaaaaaacaa aaaaggagag agatatgtca
    25561 gcgatttcaa tgaaactttt actggaggct ggtgttcatt tcgggcatca gacaaacaaa
    25621 tggaatccca agatgaaacc gtacatcttc ggagccagaa acaacattta tatcatcgat
    25681 ttgcagcaga ccgtggggct tttccagtcg gcgtataact ttgtcatcga caccgtcagc
    25741 gaagggaagg aaattctctt tgtcgggaca aaaaaacagt cacaggaagc cattaaggaa
    25801 gaagcaacgc gttgcggtat gccttttgtc aatcagcgct ggctgggcgg catgttgacc
    25861 aatttcgtta ccatcaaaaa gagcatcgat cgtctgaact cgctgacgga catgttcaat
    25921 gacgactcca ttaaagcttt tcccaaaaaa gaaattacga aactgcagaa ggaaaaggaa
    25981 aaaatggaaa acgtcttggg cgggatccgg accatcaagt ctctgccggc ggccgttttt
    26041 atcgttgatc ccggcaagga gcatattgcc gtcaatgaag ctaagaaact gagaattcca
    26101 atcgtcgcta tcgtcgatac aaattgcaat cccgatgata ttgattacgt gattccggga
    26161 aatgacgatg ccatcagggc catcaaactg tttacctcta aatttgccga tgcagttttg
    26221 gaggggaaaa agcgttttga agaaaaactg gctgctgaat cgaataagga agcggatgcc
    26281 gccgtggaga ccaaaccggt ggaagcggat attccggaaa gtctggaaat tgatgatagt
    26341 atcgataaat cggaaacgta cgccgaataa tcggaatctg tctggaattt agaatagcat
    26401 gtaagggaga aaagtaaaca tggaaataac atcaacaatg gtgaaagaat taagaacgaa
    26461 aaccggcgcc ggcatgatgg actgcaagca ggcgctggct gaatcagacg gggattttga
    26521 gaaagccatc gattttctca gaaagaaggg gctttccgcc gcgacgaagc ggtcgtcgaa
    26581 agcggccaag gatggaacca ttgcctccta tatccacatg ggcggcaaaa tcggtgtcat
    26641 ggtggaaatc aactgcgaaa cggatttcgt ggcgaaaacg gaagattttc agaccatggc
    26701 ccgggacatc gccatgcaca ttgcggcgtc caatccgctt tacgttcgtc cggaggaaat
    26761 tccggagtcc gccctggaga gggaaaagga aatctaccgc agtcagttgc gcgaagaaaa
    26821 gaaaccagag aaaatctggg ataagatcat cgagggaaaa ctcaaaaaat actacgagga
    26881 tgtctgcctg acccaacaga aattcatcaa agataccgac atcaccgttg cgacactgat
    26941 caacaatatg attgccaaaa cgggagaaaa catggttatc cgtcgttttg cccgttttca
    27001 actgggtgag gaaatcaaac aataatgagc acaaagcaga aggcggcata taaaagagtg
    27061 ctcctgaaac tcagcggcga agccctgatg gggaaacagt ccaccggtgt cgatccggat
    27121 gtggccaatt acattgccga tgaaataaag tcgctttcca gaatgggtgt tcaactcggc
    27181 attgtcatcg ggggcggaaa tattttccgc gggattgaag ccagcgccag aggtatggat
    27241 cgcacgacag ccgattacat gggcatgctg gcaacggtca tcaacagcct ggccctgcag
    27301 agcgcgctgg aggaacgcgg tcttcccaca cgcgtgcagt catccatcga aatgcatgaa
    27361 atcgccgaac cgtttattca gagacgggcg atccgccatc tggaaaaagg acgcatcgtc
    27421 atatttgccg gcggcacggg gaatccctat ttttccacag acaccgcggc ggcgctgcga
    27481 gcggtggaga tccgggctga cgtcatcatg aaagccacca aggtggatgg tgtttataac
    27541 gcggacccgg tcagggacaa aaaagcggtc aagtttaata aaataagtta tattgatgtc
    27601 ttaacaaaaa accttaaagt aatggattca acggcaatat cgctttgccg tgataattta
    27661 ttgcccataa ttgtttttaa tttgcagaag aagggcaata tttgcaaagc ggtttgcgga
    27721 caaaaaatcg gaacttacgt aggagaataa tcatgaagga aaccgttttt cagaaattta
    27781 aggaagacat ggaaaaaacg attgccggat taagcaaatc gttcagcaga atcagaaccg
    27841 gcagagcatc ggtttcgctg ctcgatggta tcaaagtcga ttattacggc gctccgactc
    27901 cgattgccca ggtggcgaat gtttccgttc ctgaaagccg tctgatttta attgttccct
    27961 gggatgtttc cattatcggc gccatcgaga aagccattca gaaatcggat ctgggcctga
    28021 ccccggccaa tgacggcaag gtcgtacgac tgagcattcc gcagctcacg gaagaacgcc
    28081 gcaaagagat agccaaaaac gtcaaaaaaa tggctgaaga ggggaaagtg aaactgcgca
    28141 atgcgcgcag agacgccaac gaagaattaa agaaattaaa gaaagacaac aaaatatcgg
    28201 aagacgaact ttttaccgcg caggacgaag tacagaaact gatcgatcag tatatcgata
    28261 aaacggataa aattctggcg gccaaagaaa aagagatcat ggaaatttaa caaacgcctg
    28321 atacgaaaac gacgatgggg agtttccgac tccccatttg tttttaatac ggtgttttga
    28381 ttatttctgt ccaggcagga tgccattcaa aaaaattatc cgggaattga ctgttgaaaa
    28441 aaaatcagcg gtcgattagg atagaccatc attcaggagc atacgtgttt ggttaaaatc
    28501 gacgtcaata aattgccccg tcatatcgcc atcatcatgg acggtaacgg ccggtgggcc
    28561 aagcagcatg ccatggggag aatccggggg cataaaaagg gagcggaggc cgtaagaacg
    28621 acggtgcgga cctgtcggga aatcggcgtc aaatatctga ccctttttgc gttttccatc
    28681 gaaaactggg agcggccgga caaggaagtc aaggcgctga tgtatctgct tgaagaatat
    28741 ttgaatgccg aactggacga actgcagaaa caggaaatcc gtctgaccac catcggagaa
    28801 acggatcggt taaacgagac ggtccgggaa aaactcatcg agacgatcga tcgtacggcg
    28861 caaaatgaca agatggtttt gaatctggcc ttaagttacg gggcgaagga tgaaatcctg
    28921 gcggcggtga agactgtggt gcgggacgct caaaccggca gtctcgatat cgatcgcctg
    28981 gataaagaaa cattcagccg ttacctgtac acggctcaga tgcccgatcc cgatttgctg
    29041 atccgcacca gcggagaata ccggatcagc aatttcctgc tctggcagat ggcctatacg
    29101 gagctgtatt ttacggatgt attatggccc gattttaccc gggaggatct tctgaaggcc
    29161 attgccagtt atcagaagag ggaaaggcgt ttcggtctga ccagcgagca actgcaggaa
    29221 tagcatcccg caaaacaata aaggcagaag gtgaaaaatc atggcgaaga cacccagttc
    29281 cctgatcaag agatggctga cgggcattgt gcttgttccg ttgctcctgc tggtgatttt
    29341 cttcgggtcg gaagcgatgt ttgccgccgt cgtgattctg ttcatttgcg gaggcgtctg
    29401 ggaatatcat cacatagtgt tcggacaagg ctttgtcatg gaaaaaatcg aaagccttgc
    29461 ttttgccgtt ttaattccgt tatgcgtttt gtccggcaac catcagtatc tgacggcgct
    29521 ccttgcgctg gcggtcatgc tggtgtttgt tttatttatc gggtccatcc gggaatcgtc
    29581 ttttgatttt ctttccgtcg ccaaggtggt gttcggcatc atgtatatcc ctttcctgat
    29641 gtcccatttc attgccatca gaatgctgga ccagggtgtc tggtggatca tgtttgttct
    29701 ggtgctggcc tttgccggtg acaccgtggc gctgtatacg ggcaagtatt tcggaaaaca
    29761 caagctggtt ccgctgatca gtccgggcaa aaccgtggag ggactgatcg gtctggtggt
    29821 gggcagcacc gttgcctgtc tgatctacag ccattatctg ctgccggatg tagcggttgt
    29881 gcatgtggcg gtcatggcgt ttgtcggcgg aatgatcgga caactggggg atatttgtga
    29941 atcggccatt aagagaggtt acggtctcaa ggatgccggt tcgatccttc ccggccatgg
    30001 gggcattctc gatcgtctgg attgtctgat gttcatcgct ccctttgttt attattaccg
    30061 gatatttgtg ataggttaaa tgaaaaaaat tacattattg ggctccaccg gctccatcgg
    30121 cctcagtgcg ctggatatca ttgagaagaa tccgaagcgt ttccggattg cggctctggc
    30181 tgccggtgaa aatattcaat tgctgaagcg gcagatcgaa agattcaaac cggagatggc
    30241 cgccgtaaaa aacaaagaaa cggccgtcaa actgcgccgg gccctgagac ccggaacccg
    30301 gattcggatc agttatgata cggaaggact gcaggaaacc gcggcctgtt cgtccgcgga
    30361 catcgtcatt tcagccattt ccggagcggc aggactatta cccacactgg ccgccatcga
    30421 ggccggaaaa gacatcgcgc tggccaacaa ggaaaccatg gtgatggccg gccggatcgt
    30481 cacgcgcgca gcggcgaaaa agaacgtccg gattctgccc gtggacagcg aacacagcgc
    30541 catcttccag tgtctggaag gaagcagaaa agaggattta aagcggatta tcctgacggc
    30601 ttcgggagga ccgtttttaa acgttgcggc ccgtcagtta aagaaggtca cgttgcgtca
    30661 ggccctgcgc cacccgcgct ggaaaatggg aaaaaaaata accattgatt ccgcctccat
    30721 gatgaacaag gggctggaga tcattgaagc ccggtggctt ttcaatacgt cttttgacca
    30781 gatcgatgtt ctggtgcatc cgcaaagcct cgtgcattcc atggtggagt ttatcgacgg
    30841 ggcctgtctg gcgcagctgg gcgtaccgga tatgaaaatt cccatcgctt atgccttgac
    30901 atatcccgaa agaattgcca atgatttgaa aatactcgat ctcgtgcagg ccggccggct
    30961 ggatttttac aaaccggatc tgaaaaaatt cccctgtttg aagatcgccc gtgaagcagg
    31021 ggtcagcggc ggcacggctc cggcggtttt gaatgccgct gatgaagtgg ctgtggcggc
    31081 attgatgcag gaaagactct cttttgccga tctgcccaaa atcatcgaaa aggttctcga
    31141 tgcgcatgat gttatccccg atccttcaat ggatgacata ttgcaggcgg atgccgacgc
    31201 aagacaacaa accgaaaaaa taataggaag gataacaacg tgaccacttt aatttctttt
    31261 atcatattgc tggggattct gatttttgtc catgagctgg gacattttct ggtcgcccgg
    31321 atgtcgggcg tcggagttca aaaattctcc ctgggcttcg gaccgaaaat tatcggcagg
    31381 acaatcggtg aaacggagta tgtgctgtca tggattcctc tgggaggcta tgttaagctg
    31441 atgggtgaat ccggcaccga agaattatcg ccggaagatg aaaaacgatc attcgccagg
    31501 caaaatgtct ggaagcgaat catgatcgtc gcagcaggtc ccgcgttcaa ttttttactg
    31561 gcggtagtca ttttcattgc cgtgatgatg tacggtttgc cgacgctgac gtcggatatc
    31621 ggcgaggtcc agaaggattc cgcagcgtat caggcgggca tgcttaacgg cgataaaatc
    31681 atttctctgg acgggaaaaa gattgcctta tgggaagaga tccgggatgt cgttgcccgg
    31741 gctaaagaca gacagatcga agccgtcgtg cagcggggaa aagaaacagt gcggttgcaa
    31801 atcacaccga aattgtccaa atcgaaaaat cttttcgggg aagacgtttc tgcgtatctt
    31861 atcggggtgt ctcccgcggg acaaaccgtt atcgagagga aaagtcccgt tgatgccgtc
    31921 gtttcgtcgg tggccaaaac atgggaaatc agcaaattaa cggttatctc cgtaattaaa
    31981 atatttgaag gcgtgatttc gccgaaaacg ctgggcggtc ccatcttcat agcgcaggtt
    32041 gcaggcgctc aggtcaggga aggcatcatt ccgtttgttc tcttcatggc gattttgtcg
    32101 attaatctgg gggtgatcaa tcttttcccc atcccggtat tggacggcgg ccatatcctg
    32161 ttttacctga tcgaggtcgt caccagacgg gaggtcagcg ccaaggtgag agagatgtcc
    32221 cagcaaatcg ggtttgtcgt tttgctgatg ctgatgcttt tcgttatttt catcgatatc
    32281 gaacggctaa acataaaatt cgtcaacgat gttgtaaaat tttttaaatg atatgcggat
    32341 cctggcgttt gacacctcat cccaaacggc ttccgtggcc gtcctgcaag atgacgatat
    32401 tctctatgac gtgatcattc ataccggctt gaatcactcg gaaacgcttt tgcccgccat
    32461 tgaccaggcc tgtctgcgga ccaaaatccc ccttcccgaa ttcgatgttt ttgcctgcac
    32521 cctcggtccc ggatccttca ccggtctgcg catcggcgcc ggcacgctca agggattcat
    32581 gctggccacc ggcaagccgg cggtcggtgt ttcctccctg gccgccattg cgctgaacgc
    32641 ggagaaaacc tcgaaaaaaa tatatgcgct gatggatgcg gggcgcggcc aggtctacac
    32701 ggcctgcttc caatacgggg ccggcggcat gatcgaacaa caaggcggcg aacaggttgc
    32761 caatccgtcg gacatcgttt atgacgccga tgccgtttac gtcggcagcg gggttatcaa
    32821 atacggggcc gcatttatgc ccgaacggca aaacggaatt ctttcaacgg caaaacagca
    32881 ttgccgcgct tcggccgtgg ctgttttagg caaagcaaaa tatgaacgcg gggaacggct
    32941 gaatgccgaa aactttgtgc cgttctattt gcgctcggcg gatgccgatc agaaaaaatc
    33001 ctttttcggg aaatgacttc ccgcaggcat catgacgctt tgatcttgct gagcagttcc
    33061 cagacttttc tattgttggg attctgcgag agaacgagct gcaaccgttc gacaatatca
    33121 tcgattttga cgatattcgg attggataaa aacagggaaa aataggcatt ggccagatta
    33181 tagttggtat cctcgtgaag cggattcacc ttcagcgcct tgcggtaagt cgaaatggcc
    33241 tggatcagat tgttggtgat ttcataaacc gatcccagat cattccaggc atcggtattt
    33301 tcgggttcga tatttaaaac tttctggaaa ctcttcagcg ctctggcgta atccccgtcc
    33361 tgaaaataga ttcgtccaag attgtggatg gctttggtat gcttggggtt gatctcgatg
    33421 atgttttcat attcttccgc cgcctcgtca tacatgtcgt gtctgtcgta aagggttgcc
    33481 aggcaaaagc gcggttcgat cgcctgaggt gcgatgtcaa tcgctcgggt atacagttcc
    33541 acggcgttgt gataatcgcc ctgcatttcc agaatatgac cgatttttaa aatagcttcg
    33601 atataatcgc ttttgacctc cagggctttt tcgtaggatt tttttgcaga atccagcata
    33661 tccatctttt cataaacata gcccagatgg atataattct tcgggttgtg cggttcaagt
    33721 tcgatggtct tttgaaagca tttcaaggct tgatcaaatt ctttgttttc caggtagatg
    33781 aaaccccagt tttcataatc tgaaggcgaa tagttgtctt tattgatatt tttggaattt
    33841 gcgatattca tttcgtgatg atcaagacac gcccgattaa gacataaata tatttagatt
    33901 ttcgaatttt catccatttg aaatcaaatc actgcatgtg tgcagggctg ttgaattttt
    33961 tcaaaatgac attcttgact tcaaaccata aagataccgg atcagcaaaa gaagaatgcg
    34021 ttaactgcgg atttcccttt aaaatttgcg ccgaccagta gcccgctaat ttgttcttat
    34081 tttccttgtt ggatgactgc gcctgccgga tgacatgcga cggaatcgcc agtttaatgg
    34141 actgctgacc gatggtgacc tcttcaaaat cttcctcttc aactgcctcc atcggcatcg
    34201 gctgctcttc ataagactgt tgagccgtgt ccgcagcgcg gacgccttgc ggaggagcaa
    34261 cattacccgg agcgctcacc ggaggcggga caacgcttgc cgaaggtttc ggcggtactg
    34321 gaaccgcctg aggacggacc ggtatcggct cttcctggcg cacaggtgcg gtatatgccg
    34381 gcatctcttc gggtgcggcc attctcggtc tcatcggcgc aggctcttcc atgcgcggcg
    34441 gagccggact gacaggcggc tgctcgggca tcaccggtct gggcctgacc ggaggcgctt
    34501 catccatgac aggcggcgct gtcggtctga gcggcatctc ttcgggtgcg gccattctcg
    34561 gtctcatcgg cgcaggctct tccatgcgcg gcggagccgg actgacaggc ggctgctcgg
    34621 gcatcaccgg tctgggcctg accggaggcg cttcatccat gacaggcggc gctgtcggtc
    34681 tgagcggcat ctcttcaggg gcagccattc tcggtctcat cggcgccggc tcttccatgc
    34741 tcggcagagc cgggctgaca ggcggctgtt caggtggagc cattcttgat cttggcggca
    34801 tcaggtcttc catgcccggg tgaagggggc gccgcatcgg cggctgttcg ggcataacgg
    34861 gtctgggcct gaccggaggc gcttcatcta tgacgggcgg cgctgtgggt cttgccggcg
    34921 gttgttccgg aggagccatt ctcggtctca tcggcgccgg ctcttccatg cgcggcggcg
    34981 ccggccggac cggcgcggaa acagccggtg tctctttttc ggggaaagca ggtcgcgacg
    35041 gtttcggtat tctttccggc tcctgcttgc ggggagcttc tgcggcaggt tgtgcttccg
    35101 cggcaggcgc tttgcgtttt tgagcggatg tcttgcttaa tttctggaaa cgatcggcga
    35161 tttgctgggc cacatcaccg gatatggtct gaaattcatt cgtttcaccc gcttttatgc
    35221 acaacttgca gattttgttg atcagacgcg gcacgccatg ttcggaaaat tcccagatca
    35281 ccgggatggc atcgtcgtcg aaaatttttc tgtccgtgcc ggcgattctg agccgggatt
    35341 cgatatacgt tctgacggct tccggagagg gtagtttttc cagatgaccg tacgtgccga
    35401 tacgttgaaa gagatttgcc cgcttgggat gttccagcgt tttggcgaat tccatctggc
    35461 cgacgagcag cagagtgaaa agattccgtt catcatcctg catattggtc agaagccgca
    35521 gatcgtcaag attggaagga gacagaacgt tggcttcatc gatgacaatg acgattttcc
    35581 tgccctgatc tgccgtttca aacagaagac ggttgaatat ttcaagcaaa tcgaccttgt
    35641 ttttgacttc gcactgcttt ccggtaatct gaccgatgat ttcccgcaat aactgaacaa
    35701 acgagagcgc cggattggta attaacgcca ctttatattt ttcaggttcc agcgagtcta
    35761 tgatcacgcg aagagatagc gtttttccca ggccgacttc gcccacaata accgtaaggc
    35821 actcgttgcc ttctttgatg gcaaataaag tttcggagat gacattttcc atggattcat
    35881 gacaatccac atacatggag gcatcgggaa cattgtcgaa aggtggctta gttaaattcc
    35941 agtaatcaca atacatcttt cttctcctcg gtttcgcctt gtaaaagcaa aaaaattatt
    36001 ttcagccaat aaaacacgca cccatttatt tgtcatcgct tggctgcatg aaatcaacct
    36061 cgctgctcaa atactccagg atatcttcca gcaggaacct tctcattctt cccagtttgt
    36121 aacttttaat cttgttttca tgaaccagct tctgcaaaaa gctgcggctg acctgaagca
    36181 tctggcacac ctggttcggc gcgatcattc gcaaaagcaa cgaatcgctg aatcggtaat
    36241 tcagggacag tctcccttca ggcgttacct tgatatccaa agcgggatcg cttttcttgc
    36301 cgctgagcag ttctttgatg tattcggatt gcagtaaaag atcggattgc tccttggtca
    36361 tggtgatggc gatgacggac gacgcattgt ccacgaattc ttcgacaatt tttcgttcgg
    36421 gtatcttgat ttcatgctcc atccccacac tgcggattgt caattcaggc gtcatgcttt
    36481 cttttgttgt ccgggctgta ttctccgata atcccgcgtt gtttttaacg tcgacgcggc
    36541 tgacggcaac ttcgggggcg gccgtttcac tgatggccgg ttgcgtaacc gttactttta
    36601 cttccggttg tgtgtattct cgacgcctct ttacaatgca aatcttttcc atatgactcc
    36661 tgtggtctgg gatcgctata aattattcac ggaaatccgt ctgcaaaaca gattgatccg
    36721 gtacaataca atccatcaat aaaaaatcag tttctctcct ctaatcagtt ctcagccggg
    36781 aaaacatttg cggtctatta caatcaaaaa aagcttcaca ttcccaatct tagctggaaa
    36841 ctgtagaatt taaatttaat ttagtgcaat gtagcttatt tattaagtaa aatcaagaca
    36901 attttgttca tccatgtcgt ttcggcatgc gtgtagcctg agtttcaaaa aaatcaccta
    36961 tgaaaaaata tttattttac ggttaagcgt tttcggccca gatgtatcgg ataaaatgtc
    37021 gccagcagac acaggaaaat cgccagcgcg aaagaaagca ccgcccacag gattttcagg
    37081 gccgtgagat tttttccgag aacgtccgcc atgaaaatgg tatagaccgg tccggcctcc
    37141 aggatgatca caagcgcaat cagaccgaag cttaaaatca taaaaagcag tcccccaaaa
    37201 cccgttacga tgttggccgg attttccgat ttgaaatcgg cgtaaacggc tccgagtccc
    37261 gtggccagtc cgacaatggc gggaaccaga aaaaaaacgg tcaggacgga caacagcatc
    37321 atgaaatccg aaacgttgag gagaatgttg gacgcgacca ccaggccttc ggccatcatc
    37381 atcaggggga tataatagag aaaaaatttg atccagagaa aattgccgat gcttaccggc
    37441 gcggattgca caatccagaa cgcttcgctt tccatgctga cggccggaaa gacaaaccgg
    37501 gccgcaacgg cggcgagaac ggcagcggcc agcccgatgt tcaaaaaaga aaacacgttc
    37561 tgcagataaa tggttttaat cggataactc tccagcggca gtacggaaaa attgtagatg
    37621 taaatcacaa tcagcgccgt catcaggaaa agttgcggcc actgggagga atccctgaaa
    37681 aatgtccgga tttccttgac ggcgaacgct ttggattcac gcggcaggaa gttcagaagc
    37741 gtttcccagg agaatccttt gtaccgcaaa ggcgcaaaca gacgacgggg tgtcatctgc
    37801 gattttgaaa atcccggaaa atacatgttc cgggcaagca tactgctgat aaaaacgagc
    37861 atacaggcaa aggtgaccac caggcccgca ttcagaagcg ccgttgcgcg gtcgtttttc
    37921 aaagcggcga ggatggcgtc ggtgatccac gttgtcggca ggaccggtga actgggcgtc
    37981 ttcagcgcat tcagatagag gaccaccgat gcaaaactgt cgggattgac cagctgttcg
    38041 ggccgtgtga agcgcagaac gaaaatcaga acaatcatca tcagcgctcc cagcagaatc
    38101 agaatggttc tgatgcgtcc ggcaggcaga actttcgatc cgaacaacac caggatcccg
    38161 ctcagggcgg cggcgatcag acacatgaaa atcatgacgg tcgtaaaaat ggcgtaatag
    38221 aagatgccgg tgtgaaaaat gatcccgtac gacaggaaaa ccggaagact gaaggccaga
    38281 atcatccagg aactgtcgaa agcgctgatg atccagcgcg aaaaaaagat gtcgtttgcg
    38341 gataccggca gagaatgcag aagcggcaaa tcctgactca ggtaaaggtg cgagaggcaa
    38401 ttgatgatgt tgctgaacaa caaaagcatg aagaaggtca aaataatcat ggagacaaac
    38461 ttcatggcca gaatgtctcc gaaatcctgc acgctgtgga aatacagcag gacgcggtag
    38521 aaaatcatga agatgcccgc ccagaacaaa agaccgagtc cgccgaacat gacaaggcgc
    38581 gaccagacat ttttcctgtt gccgcgggtc agactgttga gcgaagaccg cacgcgcggt
    38641 ccgatcaggg ataaaagggt cttcatagaa acgtgttctc tttaatgatc gtcagaaaga
    38701 cttcctcaag cctcgcttcg ccgagtcggg cgatctgctt taattccttg aggctgccct
    38761 cggccagcag cgtgcccttg tgaatcagcc cgatacggtg gcagagatct tcggcaatac
    38821 tcaacgtatg tgtggaaacg aaaatagtgg tttgacgcgc cgcgaggtct ttcagaatat
    38881 ccttcaccat gtgcacggct tccggatcca gtccgaccat cggttcatcg atgatgagaa
    38941 tctgcggatc gtgtaaaagc gcggcggcga tgatcagccg ctgcttcatg ccgtgggagt
    39001 aggcttcgat cagttcatcc gcccaggtcc acaaggcgaa ttgctgcagg aggcgtttgg
    39061 ctttttcagg gaaaagaccg cggcggacgt tatacagatt agcggagaac tccagaaatt
    39121 ccatgccggt cagtttttcg tacagaaaag gtctatcggg aacaaaaccg atcatccgtt
    39181 ttccttcgac gggatttcgg gccatgtcca caccgccgat cacaatactg ccggaagtcg
    39241 gcgcaagaat gccgcccatc atgcggatgg ttgtggtttt gccggcgccg ttgggaccga
    39301 taaagccgta aatttccccc gcgttcaccg tcaggctgag tttgtccacg gcgacggtcg
    39361 ttccgtaatc cttggtcagt tcggataatg tgatcatctc gtttgtgaca gcacctccag
    39421 gtttgttttg ccgatcaccc cgattaagcg gatctgctga tcggtttcgc cgcgcagcag
    39481 tctgatatgc gtgacatccg cgggaatctg gttgaacacc gcgtcggcgg ttatccatcg
    39541 gttgatgtaa agcacgcacc aggcgtgata ataaaatcgt cccctcagat acaccagacc
    39601 ggcttcagac cgggccggaa tgccggctgc ccgcaacagg gccaccgtca gaacggcatg
    39661 ttcgttacag tcgccggatc ggtttttcag aacctccagg gcattcggca ccgacagagc
    39721 cggctttttt tcgatattca gaaaaaccca gttgatgatt ttccgtgctt tgacaaaagc
    39781 ggaatcctcc ggacggacga ttttcccgat gagcgccttg atttgcggat catcggattg
    39841 aatcaatgcg gcgggtttca gaaatacggc gatatccgca ggccaaccgc ctgcaggacc
    39901 tttatccgcg ggatcttcac gcgtgatggt cagaacgcca tcgtgataat gctgtctatc
    39961 gccgtcgaga tggagctgac gctgcagcgg cggggtaatc ccgctgatcc taatttgcag
    40021 tttgcggagt ttttccggat cggcgatgtc cacattggag gggatggaag caatttgcgt
    40081 aaaatccacg cctgcgctgc cgtcgatttc cgccagcgca ttttcccggc ttgttttttc
    40141 catggaaaga cccagcatgc cggtttcttt taacacctgt ccgtctttgt ccagccatgc
    40201 gcagtgtttg gcgcccataa aatcggcgca gtattttttc agaagcaggc gattgcccat
    40261 gatcggaata atttcatcgg cgttgcgcgt gacgttgatc gtccggaccg acatgcttga
    40321 cggatcgaaa atgctgaatc cgcgggttgt gcccttatcc atgccggcgt gaaaggccgc
    40381 gtcgtagatg ttgccgctga tatgcggcag ggcctgaagc ggcatttcat ttttttgctg
    40441 acggtttggg gcaccggtgt aaaggatcag tttgttgtcg gccacacggc cgcggatatg
    40501 aaactgaaag agactggaat ttaatttgaa agtgaaggag gaaagggtca tgtccgcgtt
    40561 tagctgtcct tcagtggcga gattcagcgc ctgcggcatc cccatggtgc tgatttccat
    40621 aaaaacattt tcgttgaagg caaatccgtt ttcatttttc gtcagcatcc ggtggacaaa
    40681 tccgattttc cggtcgttct ggaaaatcgt catccagata tcctgcggtt gttgcggcgg
    40741 gaccattttt atccccgcga ccggaggcgt ttgcgtctga aaaaaatcaa gacggatcaa
    40801 aagcaaaacg gcaaaggcaa ttgtgaccag aattccggcg gccattggcc atttaaacgg
    40861 gcgcgtcatg caaaaaacct taaaaaataa taatcattat cgattaatat acaggagaca
    40921 ccggagcgat gcaataacaa acatgggagg aaatccaaca tgtttttgta taaaaattaa
    40981 agatttattg acaagcacgg tcaattgata tatcagaaaa actcgtttaa cgacaagacg
    41041 tctgacggga aatgcagatc gtgattgccg gttatgtgga tgtgatatta aaagcttgtt
    41101 tttattcgga aaagataagt atttatgaag catgacagta ttatcgccgc tgaaggatat
    41161 ccttttgtag ttttttccct gattgtcaca gtttttgtcg cctttctggg cggtaactgg
    41221 tggttgatcc tctttttcgc tcttgtgacg gttttcatcc tctgtttttt ccgcaatccg
    41281 gagcggtctt ttccggaaga cgaaaaggtt ttaatcagtc ccgccgacgg taaaatcatc
    41341 aaaatcgaag aggtggacgt caacggcacc ctctcgggga aattcaaaaa aatcagcatt
    41401 tttatgaatg ttttcaatgc gcacgtcaac cgggctccct atgacggcaa aattgaagcc
    41461 gttcattatc atcccgggaa attcttttcc gcgaatctgg ataaagcgtc ggaagacaac
    41521 gagcgcaatg aaattctgat ccgcacgccg gacaaccgca cggtctgggc ggtccagatt
    41581 gccggtctga tcgcgcggcg tatcgtctgc tgggtgacgc cggggatgcc tatccgcaag
    41641 ggggagcgca tcggcatcat ttgtttcgga tcgcgggtgg aggtctatct tcccgtggat
    41701 tccgccatat cggtcaaatt gcaagataaa gtcaaggcgg gccaaacggt tttaggatat
    41761 ttatcatgaa cgtggaaaat gcagcgcgga aaatccgcat gaaaaagggc atctacctgt
    41821 tgcccaattt attcacctcg gccagccttt tttgcggatt ctactccgcc atcgcatcgt
    41881 tcaaggaaca ttttgtcacg gcggcaatcg ctattctggt ctccgtcatc ttcgacgggc
    41941 tcgacggccg tgtcgcccgg ctgaccaaca ccaccagcaa attcggcgcc gaatacgatt
    42001 ccctggcgga tgtcatctcc ttcggcatag cgccggccct tctggcctac agctggtcgc
    42061 tttccgttta cggcaagtgg ggctgggtgg tcgcatttct gtttgtgctg tgcggcgctt
    42121 tgagactcgc gcggtacaac attcaaatag gcatcatcga aagcaaggtc ttcaacggcc
    42181 tgcccattcc tgccgcggcg tcggtcattg cgaccaccgt gatcttcttc gactatgtcg
    42241 gagcggaagg aaaattccac aatatcttca tcatggtctt tgtcatcatg atctctttac
    42301 tgatggtcag cagcgtcaag tattacagtt ttaaagacat gtctctgctg gtccgcaaac
    42361 ccttcacgat tttcttctgg tcaaccgttc tgctcatcat cattgtcgcg gaaccggaga
    42421 taatgttttt cgtcatcatc ctgggatacg ccctgtcggg gcccgtctgg tggctgatca
    42481 agtttggctt cggcaaaccg aaggccgtcc cggaaaacaa aaacaatgca gcgaatggaa
    42541 aaccgtaagg gcatcatctt taaaaaacta tttttaaacc ggatatgaga attacaggag
    42601 gaaaagccag gggaaagatg ttgcttttcc cttcgggatc aaaggaaagg ccgaccaccg
    42661 attttttacg ggaagcgctt ttcaatttga ccggttcttt gaacgaaaaa acatttcttg
    42721 atctttttgc cggatcgggc agtgtcggcc tggaggccgc cagccgcgaa gcgcgccaga
    42781 tcgttctggt ggagaacaat caaaaacttg ccggcgtgat cagaaagaat attccgaacg
    42841 gcttgcagga cggacggttg accatcatgg caaccgatgt ggcatttgcc cttcgcgatc
    42901 tttttagaaa acagtgttcg ttcgacatca tttttgccga tccgccgtat caccgcggtc
    42961 tggtcgccgt gaccctggag ctgttgggca aatacccggt cctggcgaaa ggcggtatcc
    43021 tggtgatgca gcattccgtg aaagaaccga taccggaaac ggtcttagga atttttcacc
    43081 ggaccgatca gagaaaatac ggcgaaaacg ccttaacttt ttttaaaatg gagagagaat
    43141 aatgcaggaa gattttacaa aaagaatagc gatatacccg gggtccttcg atcccatcac
    43201 caacggacat gtggatatca tcaaaagagg gaaacggatg ttcgatgaga tcatcgtttt
    43261 gatcgcttac aatcccaata aagtctatct gttcaacatt gaagaacgaa tggaaatgat
    43321 ccgcgaaata ttcggggaga caccgggcat ccgcgtggat tgctactccg gtttgctggt
    43381 cgattatctc aaaatgtcgg gggccaacat cattctgcgc ggcatgcggg cgttgtccga
    43441 tttcgaatat gaatttcaga tggcgttgat gaaccgacgc cagaccaaac acattgaaac
    43501 cgtctttctg atgagcggct ttaaatggtt ttttaccagt tccaaactga tcaaggaagt
    43561 ggcgagtctg ggcgggtccg tcaagggact ggttccggac aatgtctatc agcgattaac
    43621 ggaaaaatac aataccaggg ggtaagttgt gcgattatca gcgagagtag gcagaatcag
    43681 tccgtcggaa accttggcga tcacggccaa agtgaatgct ttacgggctc agggaagaga
    43741 cgtcatcggt ttcggcgccg gtgaaccgga tttcgatacc cccgataata tcaaagatgc
    43801 ggcgatcaag gcgattcaga gcggattcac caaatacacg ccggtgggcg gaacggatga
    43861 actcaaggac gccatcatca acaagctcaa acgcgacaac cgtctggaat acaaacgctc
    43921 ccagatcgtc gtttcctgcg gagccaaaca caccttatac aatctcgcgc agatttattt
    43981 tgacgccggc gacgaggtca tcattcccgc gccgtactgg gtgtcctatc cggacattgt
    44041 caccctcgcg gacggcacgc cggtcatcgt caaaaccgaa gagaaggatg cctttaaaat
    44101 gaagccggaa cagctggcgg cgtccatcac cgcccgcaca cgggcgctga ttctcaacag
    44161 tccgtccaac cccacgggag cggcgtattc tcccgacgaa ctcaaggcga ttgcgcaggt
    44221 tctcctcgac aaggatattc tgatcatcac cgacgatatt tacgaaaaaa tcttctacgc
    44281 cggatttccg tttgccaaca tcgccaacgt cgagccgaaa ttgaaagaca gggtggttgt
    44341 cgtcaacggc gtgtcgaaaa cctatgccat gacgggatgg cgcatcggtt acgccgcggc
    44401 ggcggaagaa atcgcctccg cgatcagcaa aattcaaagc cagaacacat ccaatcccac
    44461 atcgattgcc cagaaagcgg cggtggaagc gctgtcctgc gatcagacga tggtgggcga
    44521 tatggcggct gaatttaaaa aacgcaggga ctacattgtc gcggccctca acgaaattcc
    44581 cggcatcagc tgtttctcgc cggaaggcgc cttttatgtt ttccccaacg tgtccggcat
    44641 ctacggacgt tcttgcggcg gcaaggtcat aaacggttcg gccgatttta ttgactatct
    44701 gctggatgaa gccaatgtgg cgggcgtgcc cggagcggca ttcggcagcg acagccacat
    44761 ccgtttatcc tatgccacct ccctgaaaaa catcgaggaa ggcatcaggc ggatcaggga
    44821 agcggtttcc aaactcacct aattaggagc acaaatcaat gggcggcatc tttggagttg
    44881 tatcatcggc aaattgcatc aaaacgcttt tttacgggac cgattaccat tcgcatctgg
    44941 gaacggaaca cgcgggcatg gcggtgctcg ggccccaggg attttacaag accgtgcaca
    45001 gcgtgtccca ggttcagttc aaatcccgtt ttatcgatga atataaaaaa atggacggat
    45061 tcttcggcat cggggccatt gatgacgata cgcctcagcc gctgattttc cgttcgcggt
    45121 tcggcgtcta tgcggtggcc gcggtgggac tgatcaacaa caaaaatcaa ctggtcaatg
    45181 agctgctcaa ggaaggcgcc aacttcggag aaagggccgg cggagacatc aataatgcgg
    45241 aagtcgtttc ctgtctcatc aatcgcggcg acaacatcgt cgagggcatt gcctccatgc
    45301 gcgccgtgat cgaaggatcc atctgcgccg tggtgatgac ctcggaaggc atttatgccg
    45361 cccgcgacaa attcggacga ttcccgctgg ccatcggcaa aagcaaaaat ccgcaggatc
    45421 aatcctacgt ggtggccacc gaagccagct cgtttgaaaa tctcggctat gaactgacgc
    45481 agttcctcgg ccccggcgag attgttcttc tgaccaccga cggcgttaag ccggtcaaac
    45541 cggcgggcac cgagaaaaaa atatgttcgt tcctctggat ctataccggc tatccatcct
    45601 cctcgtacga aggcatcagt gtggaaggcg cccgggagcg gtgcggcgcc tatctggcca
    45661 aacgcgacaa tatccaggcg gactttgtct ccggcgtccc cgattccggt gtcggacacg
    45721 ccctggggta cgcgatggcg tcgggcattc cctaccggcg ggcgctggtc aaatacacgc
    45781 ccggttacgg acgaagctac acgccgcctg cgcaggaaat cagagacctg gtggccacca
    45841 tgaaactttg cgccgtgcgg gaagtgatca acggcaaccg gatcatcatc tgcgacgact
    45901 ccatcgtgcg gggcacgcaa ctgaagaatc tgaccattaa aaaattatgg gacaatggcg
    45961 cgaaggaaat ccatgtgcgt ccggcctgtc cgccgctgat gttcccctgt atctatacct
    46021 cctcgacgcg gacaaccggt gaactggcct gccgcaaggc cattcgcgcg ctggaaggaa
    46081 aagaaatcga acatccgtca gagtatctgg atcatcactc tgagaaacac gcgaaaatga
    46141 tcgattggat tcgccgggat ctgaacgtga cctccttgaa atatctggcc attgacgata
    46201 tgatcgcggc catcggactt ccggcggacc agttgtgcct gcactgctgg ctgggcaaat
    46261 aagcgtttga tgacggcctc gtttccgcat cgtgacggac ggtttaccgg atcaattcaa
    46321 atgtcgtcat tgacttccga ttcaatcaat ggaaaaatgg caactgtgga acattcatcg
    46381 tttatcagaa ggaaaaaaac aaaacagatt catctcgggg gcgtggcaat cggcggcgat
    46441 gctccggtga ttgttcagtc catgaccaac accgacacga ggaatgtcga ggcgaccgtc
    46501 cgacagatta aagccctcga aaaagccggg tgcgaaatcg ttcggattgc gctgccggac
    46561 gacaaagccg cagcctccgt ttccgccatc aagaaaagaa tccggattcc gctgattgcg
    46621 gacatccatt ttcatgaccg cctggccctg gaagcgatca ggcagggcgt agacggaatc
    46681 cggattaatc ccggcaatat tgcgccgggc aaaatgggcg ccatcatccg gctggcccgg
    46741 gaacgccggg tggttatccg gatcggcatc aactccggat cgctgcagaa agacctgctg
    46801 aaaaaatacg gcggcgccac gccggaggct ctggtggaaa gcgccgtgcg caatttggaa
    46861 ctgtttgagg accgcggatt cgatctgatc aaattatcgc tgaaatcttc cgacgttccc
    46921 gccacgatcg aagcataccg ggccattgcc gcccggacgg attacccgtt gcatctgggc
    46981 gtcacggagg ccggctcgct catcggggct gcgatcaaat cttcgctggg catcgggacg
    47041 ctgctgtacg agggtatcgg cgacaccctc cgggtgtcgg tcaccggcga tccggtttcg
    47101 gagatggggc tggcgtacgg cattttacgc gcgctgggca tccgccgtgt cggcgtggac
    47161 attgtttcct gcccgacctg cggccggtgc gaaatcgatc ttccgagcct cacgcgccgc
    47221 atcgaaaaag ccctggccgg ccggaaggac tatttaaaag tggccctgat gggctgcgtc
    47281 gtcaacggtc cgggcgaagc ggcggacgcc gatatcggtc tggccggggg aaaagggcag
    47341 gggctccttt tcaaaaaagg aaaagcttat aaaaaagtca gagaaaaaga ttttgtgaat
    47401 attttactcg aagaaatcga tacgatcatt gaagaaagga aaaaggatta atgcgttatt
    47461 cagaaatgtt tttaccgacc gtccgggaag tgccttccga tgcggaaatc atcagtcacc
    47521 agttgatgat ccgggcggga ttgatccgca aactgaccag cggcatctat tcctaccttc
    47581 cgctggggta ccgggtgatc agaaaactgg agcagatcat ccgcgaggag atgaaccggg
    47641 cgggcgcgca ggaagtgcat ctgcccatgg ttcaaccggc ggaaatctgg caggaatcgg
    47701 ggcggtggac ttattacggc aaggaacttc tgagactccg cgaccggcat gatcatgaat
    47761 attgtctggg gccgacgcat gaagaagtca ttacgggcct ggtgcgcaac gacgtgaaaa
    47821 cctaccgtca actgccgctc aacctgtatc agatccagac caaattccgc gacgaagtcc
    47881 gtccgcggtt cggcgtgatg cgctgccgcg agttcggcat gaaagacgcc tacagttttg
    47941 atgtcgatga agagggcgcc gagaaaagtt acgaaaaaat gtttgctgcg tataacaata
    48001 ttttccggcg ctgcggtctg caattccggc cggtggaagc ggattccgga agcatcggcg
    48061 gcaagtattc ccacgagttc atggtcatgg ccgactccgg agaagacgcg atggtgtttt
    48121 gcgaaaaatg cgcctacgcg gcgaatctgg aaaaggccga agtggcctgt cccgagaaaa
    48181 aaatcatctt tgaagacgac tggctgcccc tggaggacgt ggatacgccg gatgtccgca
    48241 ccatcgagga agtcagtgcc ttcttgaaag tcacgccgca gaacatcgtc aaaacgctga
    48301 tcttcaatgc cgacggcaaa ccctgcgccg tgctgatccg cggagatcac gaggtcaacg
    48361 aggtcaaggt taaaaattat ctcgccgcgg ccgaactgga actggcctcc gacgacatga
    48421 tccgggaagt caccggggcg ccgcgcggtt tcgccggtcc cgtcaatatc aaagccccga
    48481 ttcttgtcga ttattcggtg atcgatatgg tgaattttat cacgggaggc aaccggcagg
    48541 atggccatct gaaaaacgtg aatgttggca gggatttcaa agtcgaagcg ttcacggacc
    48601 tgagaattgt caacgccggc gatccctgtc cgcgctgcgg acagcccatc aaaacggcgc
    48661 gcggcattga agtcggccat gtgtttaagc tcggcaccaa atacagcaag gccatgaagg
    48721 cggtttatct ggacaaagac ggtcaggaaa aaatcatgat catgggctgt tacggcatcg
    48781 gtgtcggcag aaccgttgcc gccggcatcg agcagaatca cgacgacaac ggcatcatct
    48841 ggccgatgcc gctggcgccc taccaggtca tcatctctcc cgtgaatatc aatgagatca
    48901 ccatcaggga aaccgccgaa tatctctata atgccatgat cacggaaggc gtggaggttt
    48961 tgtacgacga ccgggatgag cgggccggcg tcaaattcaa ggacgccgat ctgatcggta
    49021 ttccgctgcg ggtaaccgtc ggccacaaaa atcttcagga cggcaacgtt gaactgaaga
    49081 tcagaaaaac aggcgccaat gaattgtgcc cgctgcagga catcgtcggc agggtccggg
    49141 agatcatccg gcaggaattg aatccggata tcgcctagcc gcgaaacgag agggttgaag
    49201 agttcatgct tcggatcaac gacattattg aaaaagctca gacctatctt ccggcggaag
    49261 atctggaaat gatcgagaag gcctatattt attcggcatc cgttcatcag gggcaggtcc
    49321 ggttgtccgg cgagccctat ctgacgcatc cgatggaagt cgccggaatc ctgacggaca
    49381 tgaaaatgga tgccgcaacc atcatcagcg gcctgctgca tgacaccatt gaagacaccc
    49441 tgactacggc ggatcaactg gagcacgaat tcggaaaaga cgtctccttt ctggtcggcg
    49501 ggctgaccaa gttgagccgc atttccttcg ggtcgcagga ggagcggcaa tcggaaaact
    49561 tccgcaaaat gattctcgcc atgtcgtcgg atatccgcat tctgatggtg cgcctcgccg
    49621 accggattca caacatgaga acgctgcagt atcaccccaa ggaaaaaagg atctacatcg
    49681 cgcgcgaaac catggatatt tacgcgccgc tggccaaccg gctgggcatc aactccatgc
    49741 ggatggagct ggaagatctt gccttccagt acatcgcacc ggacgcctat aacgacctga
    49801 ccgccaagat cgtcaaatcg aatgaggccc gcaaccgcta caccgaggaa gtcaaaaata
    49861 tcattcagca ggaaatggag aaatattccg ttgcctgcaa agtggagggg cgggccaagc
    49921 acctctacag catttacaaa aaaatgcatg aacagcacat caatttcgac gaggtttacg
    49981 atctgatcgc cttcaggatc attctcgatg cggacaagga aaaatcctgt tatgaagtgc
    50041 tgagcatcgt gcacgcgctg tggaagccgg ttccgggacg gttcaaggat tacatcgcca
    50101 tgcccaaggc caacaattac cagtcgctgc ataccacggt catcgggccc tacggcgagc
    50161 gggtggaaat tcagatcaga acccggaaca tgcacgaatg ggccgaaaaa ggaatcgccg
    50221 cacattggcg ctacaaggaa ggcggatcct acaaggacaa cgaagaagac cagatccgga
    50281 aactccgcga agtgctggag atccagcagg acatcaaaaa tccgaaagaa ttcatgtccg
    50341 atctgaaact ggctctcttt ccggaggaag tctacgtatt tacgccgcac ggcgacgtca
    50401 agtcctttcc caagggcgcc acgcccatcg atttcgccta cagcatacac accgacgtcg
    50461 gcaatcagtg tatcggcgcc aaggtcaacc gcagcatcgt gccgctgcgc tacaagctgc
    50521 aaaacggcga tcgcgtggaa atcatcacgc aggcgggaca ccatcccagc aaggactggc
    50581 tcaaaaatgt cgtgaccacc cgggccatca ccaagatccg caattacatt aacctggaag
    50641 aaaagaaaaa cagcatagtt ctgggacggg agctgctgga aaaggaattc aagcggcata
    50701 atctgaaatt ttccggcatt gtcaaatcgg acgacattaa aaaaatcttt tccgcgtttt
    50761 cggtgaatca gctggacgat ctgttgttcc aggtcgggct ggggagaatt tccgccaaac
    50821 gggtcgccca ttatttcatc gccggcgaag aagtcgaagc ggcagaaccc gcccgggaaa
    50881 aaattaaaaa gaaaaaagcg gcatcgcctt ccatgggcgt ttcgctgatc ggcattgacg
    50941 accctgacga catcatggtg aattttgcca aatgctgcaa tccgattccc ggcgatgata
    51001 tttccggtta catctcgcgc ggccgcggcg tgatcgtcca taccagcaat tgcccctata
    51061 ttaaacgaat ggatcaggag cggatcgtcg acgtgcagtg gagttcccgc gaagagcaca
    51121 cctatcccgt gaacatcaaa gtcgtctgtg acgatattaa aggcatcctg acggaagtca
    51181 gctccatcat ttcatcgttc gacgtcaata tcagctatgc cgaaatcgaa accgtcaaca
    51241 tgatcgccac ctgtaaattt atcatcgaca tcagcaatct gccgcagttg aaccaaatca
    51301 tgtccgccat caggcagttg aagtttgtca aggccgtgga atggctgcat catttttaaa
    51361 tcatattgac aaaaaaaatc actctgttat aaacaggccc gactaaaaaa aagagagaag
    51421 acgatgtttc atacaaagaa aattctagtg ctcatcacgt ttgccgttct ggcctgtttt
    51481 ggctggggcg ctgcggagct tattgccgcg gagaaaaaat acatcattct gatcgatccg
    51541 gcccatggcg gaaaagatcg ggggctgaaa ctcaatgacg acgtttccga aaaagatgtt
    51601 accctggcgg tggcgctgca gatcaaaaag gaacttgcgc aggaacccaa cctccaaatc
    51661 atcctgaccc gggagacgga caaaaccctg gatcgggaag ccagacaaaa aatgatcaaa
    51721 accctcaacc cggacatggt gctgaatctt cacgtcaacg gcggattcgg caaggaagcc
    51781 tccggatatg aaatctatta tccgtcctgc ggcgccgacg atccggcctc aatgcggaag
    51841 accgacaaaa ccaaaccgca aagccagtgt cagaacaatt ctttaaaaat ggccaagctg
    51901 atttcggata atttcaacgc gctctttccg cgcaaggcca gaggcctgcg catggccgat
    51961 ttgtccgtca ccgagggggt gctggtgccg gccgtggcgg tggaaatggg ctttgccaca
    52021 aatgccgttg accagaaaaa tctgatgtcc aaaaaaacgc aggctgaaat gggcaaggcg
    52081 ctggccgtca gcatcaaagc attttccagg tgagttgtga ccgaaagaaa atcaggccgc
    52141 cggttcgatg aactgaggcc gataaaactg atcaagaatt atctcaatac cgccgccggt
    52201 tccgtgatgg tggaattcgg caacacgaaa gtgctctgcg ccgcgtcgat cgacgaaaaa
    52261 acggcgccct ttctgaggaa tacgggccgg ggctgggtga ccgcggaata ttccatgctg
    52321 ccgatgtcca cgcagacgcg cacagcaagg gaatccgtcc ggggaaaaat ccagggacgc
    52381 acccaggaaa ttcagcgtat gatcggccga tcgctgcgcg ccgtgacgga cctgagcgcc
    52441 ttcggcgaaa aaacgattta tctggattgc gatgtcattc aggcggacgg aggaacccgg
    52501 acagcgtcgg tcacgggcgc gtttgtcgcc ttggtgggcc tgtttgatca attgaaacga
    52561 gacggcgtgg tgcagaacat tcccgtgcgc gattatctgt cggccgtcag tgtcgggatc
    52621 atcgatcagc aggtgatgtt ggatctggaa tatgaagaag attcccgcgc ggacgtggat
    52681 atgaattttg tcatgaccgg ttcggggcat ttcatcgaag tgcagggcac tgcggagaaa
    52741 atcccgttca gccgggaaca aatgacacag atgacagacg tggccgagcg cggcatccgg
    52801 cagatcatcg aacggcagaa agaacttgtg ggtgacctga tcggatgaaa atcgtttttg
    52861 cttccggcaa tcttggcaag gtccgggaaa tccgggaaat gctttcctgc atcaacatcg
    52921 agcttgtctc cctgaaagat tatcccgata tcccggaaat cgtcgaagac ggaaaaacct
    52981 tcctggaaaa cgccctgaaa aaagccagaa ccgtttccga acggacagca caggccgttc
    53041 tggccgacga ttcgggactg caggtggacg cgctgggcgg cgagccggga atttattcgg
    53101 cgcgttatgc cggagaaggg gcctcggacg acgacaacat caatctgctt ttggaaaaac
    53161 tcaaagaggt tccgccggaa aaaagaaccg cttccttttg ctgtgttctt gtattataca
    53221 acaccgacgg cacctacgaa tcttttgaat cccggtggcc gggccggatc attgacgacc
    53281 gccgcggcga caacggtttc ggctacgacc cggtttttta cgtgccggaa ctgaacaaaa
    53341 ccgccgcgga actgccgccg gaaatcaaaa acagaatcag tcaccgggga caatccttct
    53401 tgaaactgaa acagtccctc ggacggaaaa tgaaaaaata aaaccggggc gtagcgcagc
    53461 ctggtagcgc gcctgctttg ggagcaggat gtcgagtgtt caaatcactc cgccccgacc
    53521 attcaaaact ccggcggtgt aaaatctttt ttaatttatt ttgcacagtc cgctcgggca
    53581 acgcctgcgg ccggccgttg catcatcctg aaaaatgaaa cgttgcaaaa cgcatatcat
    53641 gtcgcatgga aaagcgtaaa aataattgat tggaaaataa tccaatatgg attaagatac
    53701 gcgccatatt cataaaaact ttttgaaaag ctgtttcacg aaagctcaaa aacatggggg
    53761 agggtttgca atggagcgtt tcagaaaatt atgcgtgatc attttaagtt tgagttttat
    53821 tttcagtgtt gccgcctgcg ataatttttc aaaattcaag gaaaaaatgt taagcgagga
    53881 gatcatctcc tttcccgtga agcagggaaa cagctggggc tttatcgaca cgacgggcaa
    53941 attcgtcatc caaccccagt ttgacggagc ggggcgtttt tccgacggat tcgccgccgt
    54001 gaaaatcggc gaccggtggg gattcatcga caagaccggg gaatacgcca tcaatccgca
    54061 gtttgatgtc gcttttctgt tccatgacgg actggcatcc gtcggcctgg gcggcggcaa
    54121 ggtcaaggtc ggattcatca acaaacaggg caagtatgtc atcaatccgc aatttgatga
    54181 tgccgggtgg ttttcagaag gccttactgc ggtgggaaaa atcgacgggc agaaagtcaa
    54241 ggccaaggtc gggttcatcg acaaaagcgg gaaatacatc atcaagcccc ggtttgaaga
    54301 tgcgggctgg ttttcggaag gcctcgcggc agtcaaaatc gacggcaaat acggctatat
    54361 cgacaaaacc ggcaaattca tcatcgaacc gcagtatgat cttgcttttt ggttttccga
    54421 aggaatggct acggtcaaaa acggaaataa atacggctat atcgacaaaa ccggcaaatt
    54481 catcatcgaa ccgcagtatg atttcgcttt ctggttttcc gaaggcctgg cctcggtgaa
    54541 gagcaagggg aagtacggtt tcatcgacaa aagcggccgg tacgtcatcg agccgcagtt
    54601 cgatatcgcg ttttggtatg aaaacggttt ggcgccggtc ggcatcggac gcggcaaggt
    54661 caaggtcggg ttcatcaaca aacagggcaa gtacgccatc aatccccagt ttgacggtgt
    54721 gaatgttttc taaagtcggg gagaaaaact aaaaaaagaa agggattgct tccgcacaca
    54781 gatcgaattg tttgctttgt cgccggcgat aacgccggcg gcgaggcgcg agcgctcttt
    54841 ccatgcggcc cccataacca tgtccggcac actctatccg tttaaagtca atgacagcct
    54901 ctggatactg ggcaacggct attttcatct ttatctgctg aagggcgcca aacaatgcgc
    54961 gctggtggag accggcatat ccgcgacggc ggacctggtg ctttcgcagc ttgaggcgct
    55021 ggatgtccgg ccggattatc ttgtcgttac acatccgcac agcgatcaca tcaccggact
    55081 ggaccatctg cgcggggcgt ttcccggcgc tgttgttatg attgcggcgg gcgccgcatc
    55141 gtttgtcgcg catccgaaag ccgcgccggc aattattgcc gaagaccggc acatgttccg
    55201 ggcgctggcg gcccgcggcc tgttcagcca aaccgccgtc gtcacaatgc cgccttcgct
    55261 ttccggatgc agaatcctta ccgacgggga agcccttgat ctgggcggga tgtccgtgaa
    55321 ctttctttct gtcaaaggcc attccccggg aaacctgctc gcctggattg ccggcacaaa
    55381 aacccttctg gtatcggaca gcctgggcaa ccattatccc ggacgcggct ttttcccgac
    55441 gttttttacc ggttataacg attacatggc aaccatcgat cgtctggaga gcttccggcc
    55501 gaaaagaatg gggcttgcgc acaacggatt tttttccaca tccggagcga ttgcagacat
    55561 attttgttta tcgcgaaagt ccgcacagga tgtccgccga tacatcgagg attgcgcgat
    55621 ggatgatgaa gacctcgccc gcagcctttt cggattttac tatacggacg aactggccgt
    55681 ttattctccg gacaatattt taaactgctg ccgattgctg gttcgccgtg tcagagaatt
    55741 acacacggcg tagccggccc atccgccata gattattttg agtcgacacc cgttattcgg
    55801 ttcagccgcc gctacacatg gagcagcgcc tccacctctc cgcgaatttt gttgagcgtc
    55861 tttacagagg cggcctcgaa gcgcagaaca atcaccggct gcgtattgga agagcgcacc
    55921 agcgcccagc cgttttcaaa cggaatccgg atgccgtcga tgtccacaat gccgggcgta
    55981 tttttgaaat gctgtttgat gccctcgacc acctttgctt ttttgctgtc cgtgcaatcc
    56041 actctgattt ccggcgtggc gaatgttttc ggcacatcgg caaagagacc gctgagtttt
    56101 ttgcccgtcc gggacagaat ttccagcagg cgcagcgcgg catagatccc gtcgtcgtat
    56161 ccgaaataac ggtcggcgaa aaagaaatgg ccgctcatct ctccgcccag caccgctttg
    56221 acctctttca ttttcgcctt gatcagagaa tgtcccgcct tccacatgat ggcctttccg
    56281 gaatgctttt cgatatcgtc aaaaagaacc tgtgaacact tgacctctcc gataatcgca
    56341 gcgcggggat gattcttcag aatatgccgg gcgaacagca gcagcagttt gtcgccccag
    56401 ataatctcgc ccttgtcgtt gacgacgccc agacgatccg cgtcgccgtc gaacgaaatg
    56461 cccagatccg ccttgttttt gcgcaccagc cggatcagat cggtcaggtt ttcctccacc
    56521 gtcggatccg gaaaatgatt gggaaacgtt ccgtccggtt cgcagtgtac gggaatcact
    56581 tcgcagccga accgtttgag cagcggcaca gcgaaatatc cgccgacgcc gtttccgccg
    56641 tccaccacca ccttgatcct tcgttgaatt ttaatgcgtt tgaaaagata attttcgtac
    56701 tctttggcaa tatccttttc ccggcagcgc ccggcgcctt tcgcatagcg gccgctttcc
    56761 atgattttgc gcaggtcctg gatctgcggc ccgaaaatcg tgctgtggcc gatgcagatt
    56821 ttgaagccgt tgaattccgg cggattatgg ctgcccgtga tcatcacgcc gccgccgacg
    56881 cgcaggtgac ggatggaaaa ataaagcatg ggcgtggcgc acatgccgat atcgatcaca
    56941 tgaatgccgg cggcgttgag gccgcggctc agaaaccgat gatagctctc ggagctcagg
    57001 cggcagtcgc gtccgacggt catgttcctg attcttttcg ccctggcgta cgtgccgatg
    57061 gcgcgtccca gatggaacac aaaatcttcg tttaagtcct tatcgacaac accgcgcaca
    57121 tcgtattctc ggaaaacatg gggattcatg aaacggtcaa ctcctttggt aaattataat
    57181 ttgaatttca gcgtcagctc gtaaaccccg ctgtcaattt tcttcttgat atcgaaaccg
    57241 cccttttcaa atacatgcag catcggcttg ttctcgacga ggacttccgc ggtgaacccg
    57301 agcaagccct gccgtttggc cagatacgtc aggtatttga gcaactcgaa tccgatgccg
    57361 ttgttctgct cgctgtcgcg cacagcgaaa gcgacttcgg cggtatgcgt ggcgtcgtcg
    57421 attccgtatt ggccgacggc ggagatatac gaattatcga catcgccttt gatcgccacc
    57481 agcacaatct gtttggtgta atcgataatg acgaaattct gcagccgctc atgcggcata
    57541 tccttgcgcg tcgaaataaa acgccgttcg atgcttttat ccgacagcga ataaaagaaa
    57601 tccttcaaaa gactctcgtc gctgatttta accggtctga tgaaaacctg caggccggtc
    57661 ttggtggtga gatacgtctc caggtcctcg ggatattcgc cgcgcttgcc gggaataaac
    57721 gcctggtcct tgaaaatcag gccgcgtttt ttcgcctcat cgatcagcca cggccggaat
    57781 ttcggatggg cgatggcaat cagcgacatg gcgcgttcgc ggatattttt gccgtgcaga
    57841 taggcgatgc cgtattcggt gaccacatag cgcacatcgc cgcggttcag cgtgaccccg
    57901 gcctgttcct tgagcgccgg aacgatgcgg gaaaccgtat cgttcttgga cgtggatttc
    57961 agcgccagaa tggatttgcc gtttcgggca aacagcgcgc cccgcataaa atcctgatgg
    58021 ccgccgatac cgctgtaaaa aatgccgccg atggattccg acgttgcctg gccgctcaga
    58081 tcgatttcca gggcgctgtt gatcgccacc atgttgtcga tgcgggacat catcaccgga
    58141 ttgttcgtat aatccagcgt ccgcagcgat atggaaggat tattatccag aaaggcgtac
    58201 gtggatttgt tgcccatgca gaaagacgca accgttttgc cgtgatcgat ggtttttctg
    58261 gaattattga tcacgccggc tttaattaaa tccaccagac cgtcgctcaa aagttccgtg
    58321 tgaacgccca gatttttttt gttggataaa cagaacatca cggcgttggg cagactgccc
    58381 cagccgacct gaatcgtatc gccgtcctgc accagacggg caacgttttt tccgatggtt
    58441 ataatgacgt cgcctgtttt aggactggat acttccagca gcggctcatc gtaaggaata
    58501 ataaaatcaa cgtccctgat gtggatgaat ccgtcgccgt gaatgcgggg catattggga
    58561 ttgatttgag cgataacaat ttttgccttt tccgttgcgg ctttgaccac gtccacactg
    58621 acgccgaggc ttaagtaatg atgttcatcc ggcatggagg tttgtatcag agcaacgtca
    58681 atctggacaa cgccgctggc taaaagagag ggaacctgcg aaagggaaat gggcgtatag
    58741 tcggccatgc cggcgttgat ggagctccgt gtgctgttcc cgatgaaaaa agaattatgg
    58801 cggaaattcg ctttgtattt gggctcggtg taaggagcga gtcccagcga gtaaacatgc
    58861 ataatttccg cgtcaaaaaa agctttcggg tgggattcca cataacggat catcgccttg
    58921 acgaggtatt gaggttcgcc gcagccggat gcgacaaaaa tatgatctcc gcggcggata
    58981 tggctgaaaa tctcatcttc cgaaacgaat tttttgggat aggctttctt gaagccggca
    59041 agatattctt cgaaaatttc aggttccatg gcagtctgta gccccggttt aaattaaaag
    59101 aaaaacgacg gtatcccgat gtttttcgag tattataagt taaaccgctg cccctgtcaa
    59161 ccagccttct agtgagattt gctaagaacg aacgaagaaa aatcttgttt ttttgtatcc
    59221 atcggcttcg cacaaaaatg tgaaaggtga aaagtgaata gtgaagaaaa ataagacttc
    59281 tcacatacgt tcgaagtgac acagtccgca accgttgaac cctgaacctt ttttcaccat
    59341 caactatgaa ctatgaacta tcaactatac cccggaggtc atcccgttat ttcagaaact
    59401 ccgccatgtt cgtatgcttt tcaatcaatt gcgataaaag act
//
LOCUS       DAOOLB010000005        58586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_351_length_58586_cov_45.251969, whole genome shotgun sequence.
ACCESSION   DAOOLB010000005 DAOOLB010000000
VERSION     DAOOLB010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 58586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 58586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58586
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_351_length_58586_cov_45.251969"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..2179
                     /gene="glyS"
                     /locus_tag="PLF77_01260"
     CDS             107..2179
                     /gene="glyS"
                     /locus_tag="PLF77_01260"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HPC07511.1"
                     /translation="MSNELLFEIGTEEIPAGFLSKAVVDMEEIIRKSFTDKRIAFDGI
                     KCLATPRRLVLYIADLSAKQEDQTVEKLGPAKKAAFDENGQPTKAAIGFARGQGVDVS
                     QLETITTEKGEYLGARKTIAGQPTKDLLPDILKDFMLAIPFRKSMRWADCTLRFARPI
                     HWILALYSGEVIPLKIEDIESGNTSCGHRFMSPAPFAVAGFEDYLNKARENFVIADPA
                     ERRKLILEEAEKSAAEVGGKLFYTDDLLETVSFIVEYPVIVRGGYEEDYLKIPKDVLT
                     TTMISHQKYFPVVNEAGKLLPYFIAVSNTKPRDISVVAKGNERVLRARLADASFFFEE
                     DKKVPLEDRVESLKKVVFHTLLGTSHKKVSRFRKLAVKIASKVKPSVKKNVDRAALLA
                     KADLESLMVGEFSELQGIMGREYALIAGEKPEIANAIYEHYLPIVAGGDLPQSDEGAI
                     VGLADKMDTIAGFFGVGMPPTGTADPYALRRQALGIINIILSREYPLSLNFLIDESLA
                     LLKDVLKKPAEGIKKDILEFFKGRLQNQLIAQGYAYDTVDAVLSANIDELVEVIEKVK
                     ALEEFRKHPDFEPVSIAFKRVDNILKDFKGGQFDVNLLVHDAEINLFSYFDNIKPRVE
                     KGIAEKDFAAALNKLAALRPHVDNFFDNVMVMDKDEKVRFNRQSLLAEISALFHKIAD
                     FSKIVTAG"
     gene            2281..4584
                     /locus_tag="PLF77_01265"
     CDS             2281..4584
                     /locus_tag="PLF77_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPC07512.1"
                     /translation="MVGSLDKISASAPGNSKIRKELLDITNRIHAAQNIKQILVDLKS
                     EILKLFNANSLTIYVVDKLRNEIYSMFLAGTQLKEIRLPINNKSIAGYVANTGAVINI
                     ENAYNAKELKSIEAELTFDDSWDKKTGFKTRQVLAAPIYHNNQLMGAIQIINKKVGEG
                     KFSEDEIGLALEIAEVLGVAFYNQQRFVRHRKTRFDYLLSHDLIKEDELESAWKESRE
                     KKISMENFLMQKHKISKADIGHAFEEFYNCRFIQYNDKLPIPGHLIKNLKKNFLRREL
                     WVPLEMVGDKIHVIIDDPNNIIKKDTIESLLKTKKIKYDVALEEDIIKYVNLFYNTPD
                     EEHSFTEILGRMEGEDGGPSSDDDEDLGEGVSETDSVIMQLVNKIVNDAYNRRASDIH
                     VEPNVGKKNVEIRFRVDGDCSLYQTVPFSYRAAIISRIKIMSNLDITIKRLPQDGKIK
                     FRRNTGDEIELRVATIPTQGGVEDVVMRILAKGETMPLQDMGLLPRNYNELINIISKP
                     YGMILVVGPTGSGKTTTLHAALHHINRPDRKIWTAEDPVEITQYGLRQVQVQPKIGFD
                     FAAAMRSFLRADPDVIMVGEMRDFETAKTGVEASLTGHLVFSTLHTNSAPETIVRLLD
                     MGIDPLNFADALLGILAQRLVRTLCKSCKEPYHASKDEYDEIAKSYGAEEFAKLNIPY
                     NDDFKLYRAKGCPNCDGTGYKGRMGIHELLVGSEDIKRMIQKHETVEVMREKAISEGM
                     TTLLQDGLLKTLKGFTDFKQVRRVCIK"
     gene            4883..5158
                     /locus_tag="PLF77_01270"
     CDS             4883..5158
                     /locus_tag="PLF77_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07513.1"
                     /translation="MKKTLGTMIVAAAVILFTATFSFAEYAAAGAANFPFFQLGCLIV
                     GGLIMVSLKRKYDKMYVGEVVGIFALYTILMALFTNPVIETVKTIVS"
     gene            5201..5440
                     /locus_tag="PLF77_01275"
     CDS             5201..5440
                     /locus_tag="PLF77_01275"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPC07514.1"
                     /translation="MLATHKIHQKDFNNLKQVFMLSAWGFTMVVVSFLFLYIGHLVDG
                     LLGTTPNFMLGFFILGLFLCMMRLYQEACIRRKDV"
     gene            complement(5520..6134)
                     /locus_tag="PLF77_01280"
     CDS             complement(5520..6134)
                     /locus_tag="PLF77_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HPC07515.1"
                     /translation="MIRRHKGLACFIFVLLLTAGMVLASPYFLLYATDYKKADAVILL
                     LGPDFKARHKAARELVASGMADYLIISAYNKTYRNHDQDGTRQLVLDKHAPDADSENN
                     APFPGFYEDTHIEIIKAKKVMTAYGLKSAIFVSSPYHMRRIRFMAAKEFNFNDASFYF
                     VPTRYEKAPANFWELSSSDWRKVRREYSKIIWFFIYSAWLDYFL"
     gene            complement(6188..6736)
                     /gene="amrA"
                     /locus_tag="PLF77_01285"
     CDS             complement(6188..6736)
                     /gene="amrA"
                     /locus_tag="PLF77_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HPC07516.1"
                     /translation="MELTDQEKNTLLDIAKNAIAAKINHRETPELNIDSNNLRLKRGA
                     FVTLKKQGRLRGCIGYIKAYKPLWETVQEMAVAAAFHDPRFPSLRAEEIQQLTFEISV
                     LSPLKRIADVNDIEVGRHGLYIIRRHHSGLLLPQVATEYKWDRETFLEETCHKAGLPP
                     DAWKDKETEIYIFSADYFGDIS"
     gene            complement(6813..7640)
                     /gene="amrB"
                     /locus_tag="PLF77_01290"
     CDS             complement(6813..7640)
                     /gene="amrB"
                     /locus_tag="PLF77_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPC07517.1"
                     /translation="MNPIKKSVIAGSWYPGNPAVLRRDIEKYFDAVPEMTIDGNIVGL
                     IAPHAGYLYSGHVAAHAYKLVRGRTYDAVMVVGPSHRVAFRGISIFSKGGYETPLGVV
                     PVEEALADKLKKKSPIVTDIPQAHLQEHAVEIQLPFLQVALGQFSFLPLVMGSQDAAT
                     CRELAEAIADVSADKNLLIVGSSDLSHFYNYNAAKKMDAVALGHLKHGDSQGLLQSLE
                     NGDVEACGGGPMAVVMSVARMLKADRPCLLKYANSGDVTGDKESVVGYAAAVFYRSP"
     gene            complement(7645..9966)
                     /gene="cysS"
                     /locus_tag="PLF77_01295"
     CDS             complement(7645..9966)
                     /gene="cysS"
                     /locus_tag="PLF77_01295"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPC07518.1"
                     /translation="MSTFYKNVMEGIGNTPLVEITRLNPNKKVRILAKMESANPGGSI
                     KDRTALFMIESAESRGNLTHDKIILEATSGNTGIGLAMIAAVKGYKLCLIMPESASEE
                     RKKILRAMGAELYFTPAGHGTDGAIEVAYKMLRENPDKYFGTDQFNNEDNVAAHYFGT
                     GQEIWDQTDGKVTMVVASLGTSGTAMGISKRLKELNPQIKMVGVEPYLQHKIQGLKNM
                     RESYRPGIFNKNMLDEKINIQDEDAFEMSRRLAREEGLFVGMSSGAALFVALQQARQM
                     DEGLIVVIFPDGGERYLSTELFAVKKAISTFYLFNAFARKKTLFNPIKTDEVAMRTCG
                     PTIHDTPHLGNYRRLVVSDLINRYLIYKGYKVKHVLDIVDFGDKSIRGSEKESVALAE
                     YSKKYLQTFIEDTKFLNIRQDNICVKSSENVEAMLKMVEKLTDKEYAYEKLHSVYYNI
                     SKLADYGALSNIDLGKTRKVKSVDVDDYEKDSPADFALLKRSSLKELKRGIYYKTVWG
                     NVRPSWHLECAAISQKYLGDTYDIHVSGADETFPHCENVLAIQKAFSGHQGPNYWMNV
                     ELVMVEGRKMSRSLNNALSLSGLQKDGYSGSDIRLFLLSIHYRKPVNYSAGALQTAKN
                     TVRKIHTFIRRLHAIDDGVGGNGFAETDQLIYDLKQQFAAALDDDLNIAGALAALFSF
                     IGKINEPLTKGLINKNNAVKILKALENINIVLGVMDFEVGTIHRNIEELIDKRNAARK
                     ARDWKEADSCRARLAELGVDVLDTPRGVIWRFK"
     gene            complement(10055..10471)
                     /locus_tag="PLF77_01300"
     CDS             complement(10055..10471)
                     /locus_tag="PLF77_01300"
                     /inference="COORDINATES: protein motif:HMM:NF018391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HPC07519.1"
                     /translation="MIIYDLKCENGHAFEGWFKDRENFVLQHSKKLVSCPICNSSGVE
                     IVPSSITIMGKESRTENKSRSKELSGMGALKMLHQYIDKNFEDVGDKFAEVALKIHYG
                     DEEKRNIKGTSTPREEETLKEEGVQYFKIPVAKMDS"
     gene            10669..10745
                     /locus_tag="PLF77_01305"
     tRNA            10669..10745
                     /locus_tag="PLF77_01305"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10703..10705,aa:Pro,seq:cgg)
     gene            complement(11032..12240)
                     /locus_tag="PLF77_01310"
     CDS             complement(11032..12240)
                     /locus_tag="PLF77_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HPC07520.1"
                     /translation="MPVFIWEAVTKKDEAKKGEMEAVDEITVRSLLRRQGYKSIEVKR
                     KPKDLLEYLPFLQGKVKEKNVVIFCRIFSTMINAGLPLIQCLDLLAQQEQNKAFSKII
                     KSIKEDIEGGTSLTNALKKYPEIFDELFVNLIAAGEAGGILDIILQRLSSYMEKAMKL
                     KARVKGAMTYPAAVLVISCAVVALLLIKVIPVFQKMFEGMGGELPALTAALIAASQFA
                     QSYWYLGVAILVVIYIILSRFYRTEKGRWTIDSLILKSPVFGPLLKKVAVAKFARTLS
                     TMMSSGVPILEGLSIVSKTSGNVVIEDALMKTRQGISEGQSIAEPLTKTGIFPPMVVQ
                     MIAVGEATGALDSMLSKIADFYDDEVDAAVDAMTALLEPVMMVFLGGVVGGMIVAMYL
                     PIFKLASVVG"
     gene            complement(12411..13166)
                     /gene="rlmB"
                     /locus_tag="PLF77_01315"
     CDS             complement(12411..13166)
                     /gene="rlmB"
                     /locus_tag="PLF77_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPC07521.1"
                     /translation="MEFIYGINSIRDLLRLQKSGWEKIMIASGRTGPAVQDIIAMAEA
                     HNIPVEFCERKKLDKLAGTPNNQGMIAFRQTFAYADLETVMKNRHPACGHDLILILDS
                     VMDPQNLGSIIRTAHCWGANGVIIPEDRSAGVTAAVNKASAGSVHHIPVAKVVNLAQL
                     LDHLKDRGFWIFGAETHGGKKLTETDFDCPVALVMGGEAKGIRPLLKKKCDFLTTIPL
                     TGNFDSLNVSVAAGIILYEISRQRTRSTKQSGK"
     gene            complement(13192..13944)
                     /gene="pyrF"
                     /locus_tag="PLF77_01320"
     CDS             complement(13192..13944)
                     /gene="pyrF"
                     /locus_tag="PLF77_01320"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPC07522.1"
                     /translation="MKNISSKNAGERLIFALDVQNYEDALSLVDLLSGHVGMFKIGKE
                     LFTAAGPKIIRAIKERNQKVFLDLKFHDIPNTVARAAEAAVKMHVDMFNVHASGGSRM
                     IRESVSASWTCSDALGRARPILLAVTVLTSLNNDDLKEIGFQKTTNELVVHLAGLAKA
                     AGASGVVASAQDIVMLRKAFGEDFVIVTPGIRSAAEIRKDDDQKRTLSAYDAVAAGAD
                     YIVVGRPIRTAADPLKACRQIVQEIAEGLSAR"
     gene            complement(13925..14194)
                     /gene="rpoZ"
                     /locus_tag="PLF77_01325"
     CDS             complement(13925..14194)
                     /gene="rpoZ"
                     /locus_tag="PLF77_01325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPC07523.1"
                     /translation="MARITIEDSLRVAKTRFGLVSLASKRAHQLLKSSQPLVVSKNRE
                     IVQALREIAAGKVVYANPEYLNGTKEDFKPIPDSTEFIGDEEYIE"
     gene            complement(14223..14825)
                     /gene="gmk"
                     /locus_tag="PLF77_01330"
     CDS             complement(14223..14825)
                     /gene="gmk"
                     /locus_tag="PLF77_01330"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPC07524.1"
                     /translation="MSKGLFIVVSAPSGTGKSSICQGFMEAFPEIKFSVSYTSRPPRP
                     GEIDGKDYNFISREAFRKKIRQGEFIEWVENYGHLYGSSRRFIEEGVRDGRDLLLDIE
                     PRGAKKIKQEFRRGIYVFILPPSRQELLKRLKKRGHERENVIQKRYKQSESEIKEISW
                     YDYVIFNNDLKKAVNELIAIYTAEKCKRTRLNREIREFIK"
     gene            complement(14830..15708)
                     /locus_tag="PLF77_01335"
     CDS             complement(14830..15708)
                     /locus_tag="PLF77_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPC07525.1"
                     /translation="MIKSMTGYGRAEKFFQDKNIIVEAKSVNHRFLEIVLRTPSALFP
                     MEMDYKKKITERFKRGRIEVFIKFEGEGADTAKVNLNMEIARGYFNVLNRLREEFDLE
                     SPVTLKTLTAFRDIFTPPAETEIGSDFLCEVEKSFLEALEILAAMRQGEGLVLLQDMQ
                     MRLKMIDGVLNDIAVRAPQVVSEYQKRLSERIKELTEGFALDENRLAQEVAIMAERTD
                     ITEEIVRLRSHLGQFEVLLHSDESEGRKIDFLLQEMNREINTIGSKVGDVDITRHVID
                     VKSELGKLREQAQNIE"
     gene            complement(15749..16282)
                     /locus_tag="PLF77_01340"
     CDS             complement(15749..16282)
                     /locus_tag="PLF77_01340"
                     /inference="COORDINATES: protein motif:HMM:NF025743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4416 family protein"
                     /protein_id="HPC07526.1"
                     /translation="MSKPSPAEPVKLFFSLFAQDAVIIDETIKELSRAYGDPDFISAL
                     QPFDYTDYYGGEMGENLIRRYLTMEKLIHPESLPDIKLATNDIEIKSAAGLKRRINID
                     PGYISKAHLILATGKSYTHRPYLRDGIYADLTLVYQGKKFCVLPWTYPDYADAKQLEM
                     LDRIRNRYLTQLKMTDH"
     gene            complement(16279..17268)
                     /gene="rfaE1"
                     /locus_tag="PLF77_01345"
     CDS             complement(16279..17268)
                     /gene="rfaE1"
                     /locus_tag="PLF77_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HPC07527.1"
                     /translation="MQKLLTKKRALEIIAGFPRAGVLVIGDVMVDHFIWGNVSRISPE
                     APVPVVDVQKDNILLGGCTNVLNNIHSMGGRAYVSGVVGADTIGKQLLAELNDRKIET
                     KGIVVKKDRPTTLKTRIVAHGQQMVRFDKEDRKPISPVSIHKILDYVRSRVDKIGAIV
                     ISDYNKGVVSKELIQGIKKIVDHSGIFICLDPKQSNFSVYKGVHVITPNHYEVQRAVG
                     MELNGTNNIERLSEIILKKHSFPALLVTRGEEGVSLFENGRKIVHTHFPAQAREVYDV
                     TGAGDTVIGVLALCLAAKASLKEATCLANLAAGIVVEKVGTATVSRQELIDVL"
     assembly_gap    17522..17621
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            17667..18371
                     /locus_tag="PLF77_01350"
     CDS             17667..18371
                     /locus_tag="PLF77_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC07528.1"
                     /translation="MLNIKQLRYGTDNFSYLLYGKTQAVAVDGGAWKEILDFLKQNNL
                     MLKIVTNTHSHYDHTPGNDHLLQKTGAEFIRAADFQDHQPLVINAERILVYRTPGHTD
                     DSVCFHAGSFLITGDTLFNGTVGNCFSGNMNIFFLSIKRLMDLSGETMIYAGHDYILN
                     ALAFARHLEPRNKNIDLFRNACRSRNTTYSTLDEERGINPYLRFNEESIIHLLRKNNL
                     PHATEQERWNSLMSLE"
     gene            18506..20338
                     /locus_tag="PLF77_01355"
     CDS             18506..20338
                     /locus_tag="PLF77_01355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC07529.1"
                     /translation="MQEIKTANAKKTPPVKSRKDTKEAFLYRPQPEEKHENWLQKSLF
                     LFQHNPASIVLLRLPEKKYIDINKTVLRTFGFSRQDVIGKTSDELDIFVEQDKRQIAL
                     KQLMQQGYIENFEMKFRRRDKTILDGLFSAEIIQHREKRYVLVVITDITSRNLAQQAQ
                     RENEEKYRTILETTEEGYYEIDLAGNFTFFNDPVCALLGYSENELRGMNNRQYTSRET
                     ARKVFQAFNKVYRTGRPTKEFDWEIIRKDGTKRYIEQSASLKKDSSGKPIGFRGIIHD
                     ITERKQMEDALRKSEENFRHSLDDSPLGVRIVTEEGETLYANQAILDIYGYTYVDELK
                     NTPLHKRYTAESYADFQQRQEKRLMGEAGPPEYEISIVRKDGEVRHVLALRKEIFWNG
                     QKRYQIIYQDITERRKAELKLQETLHTLRKSINTTIQVLGIASEARDPYTAGHQRRVA
                     DLARAIATEMKLPQDTIEGIRMAGAIHDIGKISIPSEILCKPTTLSELEFSLIKSHCQ
                     YSYEIIKDVESPWPLADIVYQHHERMDGSGYPRSLKGEDILIEARIIAVADVVEAIIS
                     YRPYRPALGIEIALAEIENNAGTLYDVEAVSACLKLFRNKNFKI"
     gene            20478..21230
                     /locus_tag="PLF77_01360"
     CDS             20478..21230
                     /locus_tag="PLF77_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPC07530.1"
                     /translation="MVHSALLVTLGVAITAFMSFWSVVFSVFPAADNKIHKVATLWAK
                     LLLMICNTKVDVIGKENILFGKPQIFMANHQSDFDILISLAHIPVQFRWIAKKELFAI
                     PVFGAAMRSAGYIEIDRSNREKAMQSIDEAALRIRSGKSVMTFPEGTRSREGEIKAFK
                     QGAFHLAVKSGVPIIPISIIGSGRIMPKRSLRIKPGQLKMVIGKPIDVQDVTIENRHS
                     LIEKVRNEIIKNYNAWQDIEMSPLKDLESGAH"
     assembly_gap    21252..21351
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21352..22068
                     /locus_tag="PLF77_01365"
     CDS             <21352..22068
                     /locus_tag="PLF77_01365"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPC07531.1"
                     /translation="SSERSGKRGTLNLLTVIVPGIVQEFTELFPVSRFEHLVIVQSFF
                     PGFHQPGGAFDAVLLLIIAAIPVAVIGYLFKDQMHNIFESARAAAFFLIITGILLFLA
                     DKMKKTRRDIKNMTVIDGILSGSARAVPFLTGISRSGTTITSGIFCNLNRNAAARFSF
                     LLSLPAVCGAVILKAGYLRQISSPGSYLYFAGFVCAALAGLLALKLFFLIIREARLKY
                     FAYYGWFFGTFTLLVKSSLF"
     gene            22107..24362
                     /locus_tag="PLF77_01370"
     CDS             22107..24362
                     /locus_tag="PLF77_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HPC07532.1"
                     /translation="MEKKQTDNKRTREIKGAVCLALGLFLLLCLVSYHPQDPSFTHFV
                     AGDHTTHNFTGKAGSYTADSLIRLLGAASLLFPLMLFVCSFKYFRSSDFRIGTGRLIG
                     FLFFIFSFAGIMALLISGSISLYGESLKNGTGGLTGVAIVGLLRNYFNVAGTYIILFL
                     IFVVSLTFLVDFSMISFTEKISKSGAAFLLRWKERIMAFVDFVFNRMKSEPPSPPQPA
                     IVEKAPDVTKKAPAKKVEQTHFDFTKVKSDDKFQLPPFTLLEEAPQKDARVKRDALVT
                     NSRILEKKLSDFGVEGRVVEVTPGPVITMYELEPAPGVKINKIVNLADDLALALMAPS
                     IRILAPIPGKSVIGIEIPNLKRESVFLKEVIDNDSFTQSSFRLPIALGVNFVGTPVIA
                     DLTKMPHLLIAGTTGSGKSVALNAMICSILFKAQPEDVKFLMIDPKRLELSSYEGISH
                     LMHPVVTDPKKASQVLRWTVEEMERRYKVLNDLKAKSIDAYNELLLKGLKSKTVLAMP
                     KQIDPELESGQTIDETAREEQEIKHTKLPYIIVVIDELADLMMVAPRDVEESLTRLAQ
                     MARAAGIHLIIATQRPSVDVITGLIKANFPTRISFQVSSKIDSRTIIDQQGAEKLLGS
                     GDMLFIPPGTSKLSRIHGAYVSDKEISRIVDFIKMQAQPAYDDSIEKYALAAAQIQHE
                     GDDDFDAKYDEAVALVSELGQASISLVQRYMKIGYNRAARIIEKMESEGIVGPSDGAK
                     PRKVLIRKLPG"
     gene            24359..25717
                     /gene="rimO"
                     /locus_tag="PLF77_01375"
     CDS             24359..25717
                     /gene="rimO"
                     /locus_tag="PLF77_01375"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPC07533.1"
                     /translation="MKNAEKTKINIISLGCPKNLIDSEVMGGFLRQSGHALVDKHTDA
                     DLVIINTCAFINPAKEEAIDEILTVAEAKKNRPNLRIIVAGCLAQRYGRELLDQIPEA
                     DRVIGTGEVGNIVRHVTELIRCKNRRKAVITRPVYLMNCNHRRILPASAFSAYLKISE
                     GCSNRCSYCVIPSLRGAARSRPPDDILREAERLAQRGIREIIITAQDTTGYGWNLKGR
                     PRLSDLLNDLTQIKGIEWIRLLYAHPARITADLLSTIAENDKICSYIDLPIQHIDDAI
                     LNAMKRKVNAAEIRQVIAQIRHIIPDVALRTSLITGFPGETPARFNRMLDFVRQTRFD
                     HLGVFTYSREEGTEAAGLRYHVPEKEKERRRETIMHDQMEISASINTSLIGSVQEVII
                     EGKSDRPDYPYIGRCRRQAPEIDGVTYIKGPSPDIGHIVKCRITAADDYDLFGKIIRK
                     SS"
     gene            complement(25728..26117)
                     /locus_tag="PLF77_01380"
     CDS             complement(25728..26117)
                     /locus_tag="PLF77_01380"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HPC07534.1"
                     /translation="MTANLLKIFNPVKRNKTVLMKKSLSCLQCGKCCFVDFTAYAQQE
                     DYDRWRAENRQDILEMIDHRHLFWAGDRMISSDTGNAPGECPFLYNTGKVWLCSIYET
                     RPLICRDYQPGSSELCPQWKNRKKKEE"
     gene            26299..27936
                     /locus_tag="PLF77_01385"
     CDS             26299..27936
                     /locus_tag="PLF77_01385"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPC07535.1"
                     /translation="MTTSPDRKETEQAGVSFNTKQTETTSVRPELAEMFARQKFGLDQ
                     NRPEAVEKRRQKNQRTARANVEDLCDAGSFMEYGALAMAAQRGRRSEEDLMARTPADG
                     LIAGIGTINGTFFKEEKARCMVMAYDYTVLAGTQGFFNHKKMDRMLKLAHEQSVPLVL
                     FAEGGGGRPGDVDAIGVMTAGLDLSTFAMFAGLSGKVPLVGIVSGPCFAGNAALLGCC
                     DVIIAAKNSNIGMGGPVMIEGGGLGNFKPEQIGPIDVQTQNGVVDIEVADDVEAVAAA
                     KKYLSYFQGTTPSWEAADPLQLRDLIPENNRRAYNMRTVIKALADTDSYLELRPKFGA
                     GMITGLIRIEGRPFGLIANNCLHMAGAIEAEGADKTVRLMQLCQAHGLPILSLCDTPG
                     FMVGPEIEKRAQVRHVCRMFVVGSHLTVPYFTVVLRRGYGLGAMAMAKGGFHESFFTA
                     SWPTGEFGAMGIEGAIKAGFKKELAAIEDPDQREQLYRDLVAQLYKRGKAVNIASYLE
                     IDAVIDPSETRKWIMRGIKSAATGKKQSPVHNYVDPW"
     gene            27959..28933
                     /locus_tag="PLF77_01390"
     CDS             27959..28933
                     /locus_tag="PLF77_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPC07536.1"
                     /translation="MQIQEIFSIYKEDLQQVEKCISDNAISEIKLIPEVAHHLIDSGG
                     KRFRPLLLLIASRLCGYHGEHRYAMGTVMEYIHTATLLHDDVIDQALIRRGKTSANKV
                     FGNSVSILVGDFLCFKAFMLMSEIGNNNIFELVSRMGNIMSEGEVFQLTKCGDINMTR
                     DDYLNIIEKKTAVLISASCAAGAILGSASPEKIDAMARFGKNAGMAFQITDDILDYAA
                     KGQTFGKSIGKDLEEGKITLPLIFALKQSTDEEKNTMKEILSRKKSSRKAALQVRTFI
                     EKYEGLELSRYTAAGYIKEAKRQLDVFEDCAEKKHLITVADYILSRNV"
     gene            complement(28937..32011)
                     /locus_tag="PLF77_01395"
     CDS             complement(28937..32011)
                     /locus_tag="PLF77_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPC07537.1"
                     /translation="MQNTLENSSDQNRRSGYWGLAVSASKVGIGKVLRKHQLLLEGFL
                     QFIAGRFNTSEALLEELKGTQGWINLCLGMRSEDSAIEAAIVFQDGVVRVLKTIPENV
                     ETVIIFKTSDHLLDMTDATPDEMYKMMLIGTIRTQGNIMLASLFNYLMALVFDKGQQK
                     AVDRQIEEHRKANKSVGRDVADTDCCRQERQRRKISRVAGGKVDPGVKYLEDPHLAGL
                     GLEDFPRLEQFRAEYFGKKKEAVVCHEYGKLITDFHLANGYEVDKDGKPWDPNLRKAE
                     SLKYILENRTPVIRTNDLLAGTYTTSPVSTCVGHPFSIGCYSWGELRSFSKRELMPYE
                     ISEESIHILHRHVFPYWAKRNIHELWRSRTNGGLPVQIHDRFFSVYYWKTISMSEVPP
                     GHEALIKLGTGGLIRKIEEELARDAHADDEKKNTLKAMIISLEGVNAYARNLARQALE
                     ESKTATNPQRKAELETMHRMLLKIPESPSETLHEAVQNIILMHLCLGMESTDDGPMYG
                     RLDQILQPYFESDMHKLTTPQKREAYIKQVIDILGCLYFIESSHQILAPDIGNWQNGG
                     SSPNGTITLGGVTPQGEDAVNDMTYILLKVTELLSLNNPNVHARYKPDKNSFAYLKRV
                     CDVNYITGATPCIHGDDAVMESLTARGWAVEDVRDWVVNGCVEPGIPGKHCSATSSIE
                     FNLVAVLEMALNNGKHPLMNWKLGPDTGIIGQGDFETFDDFWKAFKEQCEFLCEQSII
                     GNNQLGEIYQQHQPAPLISSMTEGCIESGRGVTRGGARYNSSGVSVIGMADVVDSLMA
                     IKTLVYDEKKITFAELKEAIDSNFAHHPRLHVMIKTRVPRFGSGNEESVTMANRVTSL
                     VHDYFASHQNYRGGPHATGWWSMANHTVYGRVTGASPSGRLAGEPFTPGLTPHPTASP
                     NLLDNLRDVAKLKPETLDNNIAFNVKIVPASSDSHERIIEIMANYADTYFRMGGMQTQ
                     FNVISTETLKDAMAHPEYYSDLMVRISGFVAYFTRLQRDLQLEVIRRAEYKI"
     gene            complement(32236..32883)
                     /locus_tag="PLF77_01400"
     CDS             complement(32236..32883)
                     /locus_tag="PLF77_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC07538.1"
                     /translation="MSKITRSEKEIEEVRNAILEEALTILREEGFEKLSMYQLGRRTN
                     MTAANLYNYFRNKDQLIIAIHKKTFELLYAMLSKAVRKEAEPMAKLKKIIKTFLTFAK
                     KYPNYYDIMFNRRVPQVSDYYGTSEESLAREEYESSARNLNLVMSIGKNVLSSSSEPD
                     VRDVEFYFIRTFSELHGIVSLYNSGILKVIVKNPERAMHRIVDDLLTAYIRRKYR"
     gene            complement(32897..34720)
                     /locus_tag="PLF77_01405"
     CDS             complement(32897..34720)
                     /locus_tag="PLF77_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07539.1"
                     /translation="MTKSFAENRDTKKNLKKYYIMYDPTTVDLMGKIGVAGFTVEDTV
                     EYLTGRKLEACNETNYTEFLSFNTNLLRKGMLEQMAIEPYKAYYIANQICRADQDARV
                     IIADDTRKTIGQIIAEEKQKPVAVFMSLISSTFPTACAVILILNKVNIPVVIGGIHVS
                     TSFNDVDMYLRKYLPHPQIVTQVIGAGDLPTIKNVMSDLARSNLKKEYHGNIPIEDGV
                     WGCDRVIELPKIKPYFINKFPVIGPLLSRIIETNVTTPFLGCPFSCSFCSISSFPEER
                     RKFTSRSPEDFTCELLDKQKKGVTFKNRFYFISPDNLLFGGKKLHDVLDKMIESPLRI
                     NYAAQISIEVADSDKLLQKLRLSGASHFFIGLESLDIRNLEYVGKNIVAKIKKEKISV
                     EEYYASRIKKIQDHGISVHGAFMFGMPYDYFHSLEDHSGRKIVEFCKKNKIGIQPTCL
                     SNLPGSLDFLKGLETHELIYGNPGSMDYFCSLTIADLTESNRKIPDALFNSPLVTFYM
                     LYDTMEKIGSYISALHIAFGMAKKSWSVHSSKGAQSFKDRMVDAFAGIGFQLGASAYF
                     ELYKGLAYSTSSLPGTFERLYQWEKNPTVRKMFKKYVENFV"
     gene            complement(34772..36115)
                     /locus_tag="PLF77_01410"
     CDS             complement(34772..36115)
                     /locus_tag="PLF77_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPC07540.1"
                     /translation="MQINRLTLKFSGELSYLEAPFLKEYYTSSLPHIRMMMILGAMLY
                     AAFGVLDVLLMPQQVVALWIIRFIVIGPVLVGVLAISFLPIFEKYIQPILALVYFLAG
                     AGIIGMIVVAPPPVSYSYYAGLMLVFMWGYTLIRLFFTWASVAGWLQVIFYETVTVGL
                     NPTPLDVFLGNNFFFISANLVGMMACYAIEFYARRDFLMNQQVEEEREKVSRINEELE
                     DRVKQRTEDYKNLNQALLQEVAGHEQAKEKLQEALDSLKKSMGVTIQVMVSAVEARDP
                     YTAGHQIRSSNLACAIAAEMGLPQEKIDGIQMASSIHDIGKLSIPAEILTKPTKLTNL
                     EYSLIKEHSACGYEMLKNVESQWPLAEIIFQHHERMNGSGYPRQLKGDEILLEARILA
                     VSDVVESMASHRPYRPSLGIGAALEEIEKNRGILYDSAVVDACLKLFREKNFYLE"
     gene            complement(36168..37733)
                     /locus_tag="PLF77_01415"
     CDS             complement(36168..37733)
                     /locus_tag="PLF77_01415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPC07541.1"
                     /translation="MKIASVGEMRSMDRFAIEHLGIPEDVLMENAGRAAVSVLLSRTG
                     IRGKKFAIFCGSGNNGGDGLVVARLLHSRGGLVRVFLLNDSEKFRGAAATNFEIVKRL
                     PLETAQLTNARPAGRHVAHCDVVIDAILGTGLDRPVSGLVADTISLINKGGKKVLSLD
                     IPSGVNGNTGEIMGAAVTADYTVTFGLPKIGNLLYPGCDVNGELWVSHISFPPSLYDR
                     DDIPVRTNDAMALPPRPADAYKGAMGDVLFIAGAVNYYGAPYLSAMSFLKAGGGYARL
                     AAPRAVIPVIARRGREIVYLPQEETEAGSMALKNKKALLEHSAKADMVVIGPGLSLQD
                     ETVNLVREIAATVRKPMLIDGDGLTAVAGCPDSIRRRKAATILTPHMGEMARLTGKSL
                     KEIHADKIKVLRETVQTLNACIVLKGPHSLIGTRDGNVYVNLSGNAGMATAGSGDVLT
                     GCIAAMFGMGLSFDEATRKGVFLHGVAGDLAALKKGTDGMTAQDIMNALPQALKEDRS
                     GRLQDQYALPDGV"
     gene            complement(37890..38921)
                     /locus_tag="PLF77_01420"
     CDS             complement(37890..38921)
                     /locus_tag="PLF77_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HPC07542.1"
                     /translation="MKAAILEQTTSLKKNKKPLKITEVAEPAVGDGDDVLVKVSVCGV
                     CHTELDEIEGRIFLPSFPVILGHQVVGVVAGQGRRARKFAIGDRVGIGWINRSCGQCP
                     FCLSGNENLCAGFKATGRDVSGGYAQYLTVSEKFAFPIPEIFSDSQAAPLLCAGAIGF
                     RSLRLANLTNGQRLGLTGFGASAHLVLKMARYLYPQTPLFVFARNPAERQFALDLGAV
                     WAGDTSDESPEKLHAVIDTTPVWKPVVEALKNLEPAGRLVINAIRKEDTDKDYLLQLN
                     YGRHLWMEKEIKSVANVALCDIENFLKLASDMRIVPDVQAYPLNDVNQALGELKEGKI
                     RGAKVLIIS"
     gene            complement(38918..40309)
                     /locus_tag="PLF77_01425"
     CDS             complement(38918..40309)
                     /locus_tag="PLF77_01425"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPC07543.1"
                     /translation="MIYKDKNELQKKYEKIIAIEGNDVSILNETALRGDLIDHLIYTA
                     VFSADQAAQQAACWLIRRAGAALGIVSASIQPLYEAMGREDVRGFTVPAINLRGLTYE
                     SAQAVFRAMMKGKVGPVIFEIARSEIDYTSQRPVEYTAAVIAAAIKTGYRGPVFLQGD
                     HFQINAKKFAADAAKETQTVKNLIWEAIEAGFYNIDIDTSTLVDLSKPTVTEQQQTNF
                     SLAAELTTMIRDLEPEGITISVGGEIGEVGGKNSTVEELEAYMDGYLAELKKQGESLK
                     GISKISVQTGTTHGGVPLADGSVAKVKIDFAVLEKLSAVARSKYGLAGAVQHGASTLP
                     DEAFDRFPAVGTAEIHLATGFQNIIYDSSAFPADLKARIYDHLKTAFQSEWKDKDTEE
                     QFIYKTRKKGFGPFKQDMWHLPADTRDKISAGLEKQFAFLFDKLKMNNTQDVVEKYVQ
                     PVDVAPDVPVSLK"
     gene            complement(40396..41337)
                     /locus_tag="PLF77_01430"
     CDS             complement(40396..41337)
                     /locus_tag="PLF77_01430"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF012536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HPC07544.1"
                     /translation="MGEGIGDYTKNIVDLRRHMWMAGVEVELRRLIWQIAVVGKYISA
                     KIHESNRKLAGLKNVYGEEQMALDRASDEILKNQLEFSGFVREYASEELENIVKIGTG
                     QEKYIVTADPLDGSSLVDTNLAIGTIIGIHKESVLDCGRNTMVAALYITYGPLITMIY
                     SAGKGAHEFVLNREGEYVLSQENIKLKEKGSIFSPGGLRRDWNPAHLQFIDKLENDGY
                     KLRYSGGFVPDINQVLIKKGGLFTYPALKKSPKGKLRLLFELQPMAFIMEQAGGLAID
                     GDQDILSVKVETINQLSPIYIGSTYEVQLAKEMLSAS"
     gene            41676..>42010
                     /locus_tag="PLF77_01435"
     CDS             41676..>42010
                     /locus_tag="PLF77_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPC07545.1"
                     /translation="MTSSKSYKIAVVPGDGTGPEVVAEGLKVLKAVAASESIRYELVH
                     YDLGGERYKKTGEILPDSVLEEFRKFDAIYLGAIGHPDVKPGILEKGILLRMRFELDQ
                     YINLRPVKL"
     assembly_gap    42011..42110
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <42111..42898
                     /locus_tag="PLF77_01440"
     CDS             <42111..42898
                     /locus_tag="PLF77_01440"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPC07546.1"
                     /translation="RMRFELDQYINLRPVKLYPNVPCPLKDKGPKDIDFVVVRENTEG
                     LYTGGGGFLKKGTVDEVAIQESINTRKGVERCLRYAFQYTAKRNKANKLTLCGKTNVL
                     TYAFDLWERTFNELAKDYPQIKTDYVNVDATCMWMVKNPEWFDVIVTDNMFGDIITDL
                     GAMVQGGMGIAAGGNINPEGVSMFEPIGGSAPKYTGKNIINPLAAICAGGMLLETLGE
                     EKAAKRVEAAVVKLLESGKLKSLDAGKMGMSTSEIGDWLSDNI"
     gene            43306..44538
                     /locus_tag="PLF77_01445"
     CDS             43306..44538
                     /locus_tag="PLF77_01445"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC07547.1"
                     /translation="MNNFFIFIFVFVLSIAVIISFIYTMWRMNRDEQKLISIIHGSPI
                     PTFVISKNHKVVYWNRALQAASKIKPSDVLGTDQHWRAFYTSPRPCLADLILDGKTAQ
                     IAELYPDRWSRSKLLEDAYEVTQFYPDMGEKGKWYRITSAGLKDSRGNLFGVMETLED
                     ITEQKLAQDELLRRTKLESLGTFADGVAKDFDSLLTAILRNVFLAKISASDEDKMLEN
                     GLAVAERAGLQAKELAHRLITFAQGGYPVRKLEKIEPVLHEAAESVFKNSAITCNITI
                     AEDLWPCEIDATQIKQVIENVLINAREAMPDGGSVQMTTENTTTGTNIRSLGQGNYIR
                     IIIKDSGIGIPRENLPRIFDPYFTTKKTKEHGGIGLGLAISDSIIKYHNGLITVESIV
                     NSGTTFSIYLPAKTTNGS"
     gene            complement(44593..45960)
                     /locus_tag="PLF77_01450"
     CDS             complement(44593..45960)
                     /locus_tag="PLF77_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC07548.1"
                     /translation="MVVEKAVLIVDDDSVHRMILKKHLNDWGYQVNEADDGHVAVEAV
                     RRMPFDLIIMDICMLNVSGIEALGQIKAINPETPVIIMTAYASLETAVSALKKGAYDY
                     LTKPLNFDELRIAIGRATEHNRLKRENRYLRNRLDEKFNRQNLIGQSYPMVKLLETVA
                     QVAPTEANVLISGESGTGKELIANAIHFNSMRAETVFIKINCSALTETLLESELFGHE
                     KGAFTGAERRREGKFLQAHGGSLFLDEVSEMSKAMQVKLLRVLQERELTRVGGAEVIK
                     VDVRVIAASNKNLKEEVQNGNFREDLFYRLNVVSLNVPPLRERKEDIPILAQHFLELF
                     AGRNKKTIKAFTPRAMEKLLAYHWPGNIRELMNAVERSVVLSRTDYLDADDMTLVMEK
                     SHEVSGIAEQGEAIRNLSLESMEKKNILAVLADCRGNKSETARRLGITRKTLRAKLIK
                     YEMNR"
     gene            complement(46430..49087)
                     /gene="mutS"
                     /locus_tag="PLF77_01455"
     CDS             complement(46430..49087)
                     /gene="mutS"
                     /locus_tag="PLF77_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPC07549.1"
                     /translation="MAVGATLDLTPAMKQYVKIKQQHPDCILLYRMGDFYEMFFEDAV
                     TAAPVLEITLTSRNKGKEDSVPLCGFPYHAASAYITKLVEKGFKVAICEQVEDPKKAK
                     GIVKREVIRIITPGLVVDEENLAPGENNYLACLCAEKNTLGLAFLDISTGEFQIGEFT
                     DRDYFFTVISGLDFKELLLPRNFPDRILLDTIEAQMPQLRINDLDENYFLTGQAGDVL
                     QRTFSTDVLDACGISEHPAALKASGAILSYVNETQKINPQHISAIRWYSAEKFLLLDD
                     TARRNLEIFTSIQNNTAAGSLFSLFSETQTPMGTRRLRWWMSYPLVDAEKIKTRLAAV
                     AELKDDPLRRANLRKTLSRIYDLERLAGRVSLKAANARDLVALKNSLLAVPEIKSLLS
                     DCTAPAICAIRSHITEMTDVVDVISRAIVDSPPQKIQDGGFIAAGYDEELDKLRLISR
                     DGKQWIAALEAEERKKTGINSLKVGFNNVFGYYIEVTKANAALVPDSYIRKQTLVNAE
                     RYINQALKEFEQTVLNAEERIKQKEQELFINLRDGLDRYIAEIQGNANLIADLDALAA
                     LADVAEKYHYVCPEIDDEETIDIQDGRHPVVESALKNEGFVPNDCLMDLRQNKLLVIT
                     GPNMAGKSTYIRQVALIVLLAQMGSFVPAAKAKIGIVDKIFTRIGASDSLIKGQSTFM
                     VEMTETAEILKNATPRSLVIIDEVGRGTSTFDGLSIAWAVAEYIHDFQGRGVRALFAT
                     HYHQLTDLVVTKPGAKNYNIAVKEWGEKIIFLRKIMEGGTSRSYGIEVARIAGVPADV
                     IMRAKEILRNLEKGEFDEIGMPRIVRGAREGKNANPQLSLFSRRETAIALEKKGQNLI
                     EALKNIDISSLSPLEALNKLNELKNKISD"
     gene            complement(49078..49482)
                     /locus_tag="PLF77_01460"
     CDS             complement(49078..49482)
                     /locus_tag="PLF77_01460"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HPC07550.1"
                     /translation="MIITRWLIITAAILLASQFVPGIEVDKLSTAVIAACVLGLINIF
                     IRPIVVILTLPLSILTLGVFYFFINAFLLELVAYFVSGFAIKNFFSAFAGSLIISIVS
                     WLANSFITSHKIVKKDDPDYIDLRKGDDGKWR"
     gene            complement(49547..50386)
                     /locus_tag="PLF77_01465"
     CDS             complement(49547..50386)
                     /locus_tag="PLF77_01465"
                     /inference="COORDINATES: protein motif:HMM:NF018352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HPC07551.1"
                     /translation="MHIRYTVTVFLLIIYFSPGKAIGEEKPLWEIGAGMAFLQMPDYR
                     GADESRFYPLPYPHVVYRGDFFKVDENRITGQIFKTDRVLLDFSIFGSVPVNSDDNAA
                     REGMDDLDPTFELGPALKIKLWEHKQHKTRMNLTLPARAFFSTDFSSVRHEGWVFSPR
                     INIVTDDLIPRTGLNLGVSAGPMFADSGYHEYFYEVEPRDARVTRPVYRADGGYSGST
                     VTIGLSKTYKHFIFHAFVSADFLQGASFEDSPLVKRETSMMSGFNVSWIFFKSEKMVN
                     AEK"
     gene            complement(50467..50961)
                     /locus_tag="PLF77_01470"
     CDS             complement(50467..50961)
                     /locus_tag="PLF77_01470"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPC07552.1"
                     /translation="MDIKEALSQAYTGEAKAALRLKVYAQKAEDEGYKQMAKLFRVIA
                     FSEEIHGARALKGLKEIKSTEENLAASFESETQVAEVAYDRFVKLAEAEGNKEAVLHF
                     SQSKDVEETHAKLYKQAMNHFMEERETDYFVCKICGYVADGVLPEECPVCGAKAKMFV
                     AFQG"
     gene            complement(51094..51252)
                     /locus_tag="PLF77_01475"
     CDS             complement(51094..51252)
                     /locus_tag="PLF77_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPC07553.1"
                     /translation="MKKYVCGVCGYIYDPAEGDSEGNIPAGTPFEKLPENWVCPVCGA
                     AKDEFSPE"
     gene            complement(51284..52495)
                     /locus_tag="PLF77_01480"
     CDS             complement(51284..52495)
                     /locus_tag="PLF77_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPC07554.1"
                     /translation="MNDIYSAVKVTDHVYWVGAIDWTIRDFHGYTTPRGSTYNAYLVM
                     ADEITLIDTVKKPFMDEMLARIKSVVDPSKIKYIVSNHSELDHTGSLPDVIDFIKPQK
                     VFASGVGTKTLKDLFHDEREIIAVENGQSLNLGNMNLTFMETRMIHWPDSMFTYLDVD
                     ELLFSQDAFGMHLATLERFADEIPVSTLEYEGATYYANIVLPYSPIVLKALEKVAASG
                     LKISVIAPDHGPIWRKDIGWIIDLYKKWALQKPTAKAVVIYATMWHSTEKMARIISEA
                     LAQAGVKVSLISMNETHRSEAVYEVLDAGALIVGSPTLNNNILPQMADVMTYLKGLKP
                     VNKIGAAFGSFGWSGESVKHLEAMLKEMKVDIASESVAVKHVPGADELDQCQKLGKTV
                     AAKLLEKVNSQ"
     gene            52847..53788
                     /locus_tag="PLF77_01485"
     CDS             52847..53788
                     /locus_tag="PLF77_01485"
                     /inference="COORDINATES: protein motif:HMM:NF024786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC07555.1"
                     /translation="MRKDIRNPDIHSSPWLLDLAWFYIQRKMLKRKLPLLASFKITYR
                     CNLSCRACPFHLRSDREKEHMSRDAAVHIMDLLNQLGTRMIVFEGGEPLLWRDGSYRL
                     HDLILYARKKFLRVAVTTNGTMPLDVPSHTLWVSLDGQRETHNTLRSNSFDQVRANIQ
                     NTRHPRVFIHYTLNRRNWQDLPFVAQWVQDTPALKGMTVQFFYPYHQGEDDLSLPPEE
                     RRAAIEKLLQLKKSGLPILNSAGRLRAMIDNRWSCHDDILINVDPDGTITKGCYVKNR
                     GNINCDACGFTPVAEASGALDFIPGSLYAGWRLFLKM"
     gene            complement(53864..55924)
                     /locus_tag="PLF77_01490"
     CDS             complement(53864..55924)
                     /locus_tag="PLF77_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC07556.1"
                     /translation="MSDKINIGPSGRNIIFCMVLTALILAAYLQVHQFDFVNIDDSVY
                     VTENDYIRSGLTLDGIRWAFTTLYAEFWHPLTWLSLMLDYELYGLHAGGYHLTNLFLH
                     IISSLLLFGMFQRMTGAFWPSAFVAAFFSLHPLRVESVAWVAERKDVLSVFFWMLTLY
                     FYALYTEKPVFRRYLAAVFFFACSLMSKPLTVTLPVIMMLLDYWPLKRFASRRQNLFL
                     WQLKEKLPFFLLSAVFSLIAIYAQPKFPIDGWPFSLESRIINAIISFVLYLQKLFWPY
                     DLAICYPFFGRAPAGQIIAAVLLFFAVSAAVVWMMKKSPCLLVGWLWFIITILPVIGI
                     IPAGNNAMADRYLYLPSIGIAVMIFFGIPLLVENEKIKKFFLPPAALIFFAVLSGMTW
                     HQCGFWKNSEALFRNASRINEENALAHINYGNALAGEGRLEEAVVYYNKAWGMLPETG
                     GNVMAYNKMGIAENKLRQLIDVSLGNVYTKQKKYQEAVHHYTRAIAMRPITPDHVRTY
                     NFRGTAYSRLGRKKDAIRDFNEAIRLDRRDYRAYNNRGLLYAETGMHRQALQDFNQAV
                     LMNPNNAELYKNRGIACASLKLYQNAFEDFEKALTLVPDDANIYYNRGVAYIELGRHQ
                     EAFSDFNEAIRLKADYTNAYHYRGGIYLMYGYAASGCDDARKACQLGNCEILEAAGSK
                     NLCR"
     gene            56250..57134
                     /locus_tag="PLF77_01495"
     CDS             56250..57134
                     /locus_tag="PLF77_01495"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF006143.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HPC07557.1"
                     /translation="MKAEDIKKILIAGAGTMGRSIGLSCVTSGYAIVLYDINDDIPAK
                     ARDIMNKKIDKMADFGMMPKDKAESFKNNITATTDLAAAGADADVVSESIPEDPDIKG
                     DFFAELNKVCPARTIFTTNASSLVPSMFADKTGRPEKFLAWHFHPGFKLVDVMGHPGT
                     SPETVETVQRFSELIGHSPIVLKQEKAGYVFNSLLNPWFLAALNLASRDIASPEDVDR
                     SWKEITASPLGPFEMMDYVGLETVWRITDFWAKKRNNHQAKLSADLLKKYVDRGELGM
                     KTGKGFYDYTQKKSPGES"
     gene            complement(57207..57950)
                     /locus_tag="PLF77_01500"
     CDS             complement(57207..57950)
                     /locus_tag="PLF77_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07558.1"
                     /translation="MDADHGSSGKVTYNYRFIFKDGVEKEFNIALDSVTLNLLPTDKK
                     NYPEWTKLNCFRCSHCTLNDQCEYCPIAINMVDVVDYFRNYFSYDIVETIVTAQERKY
                     FRTVSLQHGVSSLIGIYMVTSGCPLMETLKPMVRFHLPFATIEETKYRAISMYLMAQY
                     FLYQHGRQPDWDLKKLAEAYENVRIVNEYFCKRLRMIQGQDASLNAVVLLDIFADSVN
                     FSMDGRMANDLEYLFKGYFESTPGANNAS"
     gene            complement(57982..>58586)
                     /locus_tag="PLF77_01505"
     CDS             complement(57982..>58586)
                     /locus_tag="PLF77_01505"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPC07559.1"
                     /translation="SLKKNPTNIIITGVGGQGNVMASRVLAGMLVNAGFIVTIGETFG
                     MSQRGGSVMSHLRVSSSSVLSPQIPQGRGDIVIALEPVEALRVLTKYGNPDVAVLTNS
                     RMVYPMGVITGEFTYPTIEEVKSMFEKISAKNWIIDATSVAVELGNPVLSNIVMIGAL
                     AGTSLLPIDRRAFEKEIAKTIPAGKHQINLKAFDAGVKML"
BASE COUNT        14855 a        15136 c        13431 g        14864 t
ORIGIN      
        1 acctttaggt tattcgcctc ggggataatt cgaccatcaa atttcagtac acttcgtttc
       61 tgaaatttgg gtctgattaa agagataaaa aatatctgga gacaaaatga gcaacgaact
      121 tctttttgaa attggcaccg aagaaattcc cgcaggattt ttatccaaag cggtggtgga
      181 tatggaagag attatccgca aatcgtttac ggataaacgc atcgccttcg acggcatcaa
      241 gtgcctggcg accccgcgcc gtctggttct ttatatcgcc gatctgtctg caaagcagga
      301 agaccagact gttgaaaaac tgggaccggc caaaaaagcc gcgttcgatg aaaacggtca
      361 gccgacgaaa gccgccatcg gttttgcccg gggacagggc gtggacgttt cgcaactgga
      421 aaccatcaca accgaaaagg gcgaatatct cggcgcgcgt aaaaccattg ccggacagcc
      481 caccaaggat ctgctgccgg acattctcaa agatttcatg ctggcgattc ctttccgaaa
      541 atcgatgcgc tgggccgatt gcacgttgcg tttcgcgcgc ccgatccact ggattctggc
      601 gctgtacagc ggcgaggtca ttcccctgaa aatcgaagat attgaaagcg gcaacacctc
      661 ctgcggccat cggtttatga gcccggcccc ctttgccgtc gccggctttg aagattatct
      721 gaacaaagcc agagaaaatt ttgtgatcgc cgatccggcg gaaagaagaa aactgattct
      781 ggaagaagct gaaaaatcag cggcggaagt cggcggaaaa cttttttaca cggatgatct
      841 gctggaaacg gtcagcttta tcgtggagta tccggtcatc gtgcgcggcg gttacgaaga
      901 ggactatctg aaaattccca aagacgttct gaccaccacg atgatttccc atcagaaata
      961 ttttccggtc gtcaatgaag cgggaaaact gctgccctat ttcattgcgg tcagcaacac
     1021 gaaaccccgc gatatttccg tggtggccaa aggcaatgaa cgggttctgc gggcgcgact
     1081 ggccgacgct tcgttctttt ttgaagaaga caagaaagtt ccgctggaag atcgtgtgga
     1141 atcgctcaaa aaagtcgtct tccatacgct gctcggaacc tcgcataaaa aagtttcgcg
     1201 tttccgcaaa ctggcggtaa agattgcatc caaggtcaaa ccgtccgtga agaaaaatgt
     1261 cgaccgtgcg gcgcttttgg ccaaggccga tctggaatcg ctgatggtcg gtgagttctc
     1321 ggaactgcaa ggcatcatgg gccgtgaata tgcgctcatc gccggtgaaa aaccggaaat
     1381 cgccaacgcc atttatgaac attatttgcc gatcgtcgcc ggcggagacc ttccgcaatc
     1441 ggatgaaggc gccattgtcg gccttgccga taagatggac accatcgccg gcttcttcgg
     1501 cgtgggcatg ccgccgaccg gtacggccga tccttacgcc ctgcggcgtc aggcgctggg
     1561 cattatcaat atcattttat cgcgcgagta ccccttgagc ctcaactttc tcatcgatga
     1621 aagcctggcg cttctgaaag atgtcttgaa aaaaccggcg gagggaatca aaaaggatat
     1681 tctggaattt ttcaagggac gtctccagaa ccagttgatt gcgcagggtt atgcttacga
     1741 taccgtggac gccgttttat ccgcaaatat tgatgaactg gttgaggtca ttgaaaaagt
     1801 caaggcattg gaggaatttc gcaaacatcc cgatttcgag ccggtctcca tcgctttcaa
     1861 gcgcgtggac aatattttaa aggattttaa aggaggtcag tttgacgtaa accttcttgt
     1921 tcacgatgcg gaaatcaatc tgttttctta tttcgacaat atcaaacccc gcgtggaaaa
     1981 aggcatcgca gaaaaagact ttgcggcggc gctcaataag ctggccgcgc tgcgtcccca
     2041 tgtggataat ttcttcgaca acgtcatggt catggacaag gacgaaaaag tgcgttttaa
     2101 ccgtcagtcg ctgctggcgg aaatttcggc tcttttccat aaaatcgccg acttctccaa
     2161 aatcgtcacg gccggctgat cgtaaagcgg caaccttcag gggacatgag gcgcattgac
     2221 ataaattcaa atccttgcta ctatgatttt catacagatt actaaggaat aatttgcctc
     2281 atggtaggtt ctctagacaa aatttccgca tcggcgcccg gtaattccaa aatacgcaag
     2341 gaattgctgg atatcaccaa ccgtattcat gccgcccaga acatcaaaca gattctggtt
     2401 gatttaaaga gcgaaattct aaaacttttc aacgccaatt ccctgaccat ttatgttgtc
     2461 gataaactgc gcaacgaaat ttattccatg ttcctggccg ggacgcaact caaagaaatc
     2521 aggctgccga tcaacaacaa gagcattgcc ggttatgtgg ccaataccgg cgcggtcatc
     2581 aatattgaaa atgcctataa cgccaaagag ctcaaaagca tagaagctga actgacgttc
     2641 gacgacagct gggacaagaa aaccggcttt aaaaccaggc aggttcttgc ggcgcccatt
     2701 tatcacaaca atcagctcat gggcgccatt cagattatca acaaaaaagt gggcgaggga
     2761 aaattttccg aggatgaaat cggccttgcg ctggaaatcg ccgaggttct gggagttgcg
     2821 ttttataatc agcagcgctt cgtccgtcac agaaaaacgc gtttcgatta tctgttaagc
     2881 catgatttga tcaaggaaga tgaactggaa agcgcctgga aagaatcgcg tgagaaaaaa
     2941 atctccatgg aaaattttct catgcagaaa cataagatat ccaaagccga tatcggtcac
     3001 gcttttgaag agttttataa ctgccgcttc attcaataca atgataagct gcccatcccc
     3061 ggacatttga tcaaaaattt gaaaaagaat tttctacgcc gcgagctctg ggtgcctctg
     3121 gaaatggtcg gagataagat tcacgtcatc attgatgatc ccaacaacat catcaaaaaa
     3181 gatacgatcg aaagtctcct gaaaacaaag aagattaaat acgatgtcgc cctggaagaa
     3241 gacattatca aatacgtcaa tctcttctat aacaccccgg atgaagaaca ttccttcacc
     3301 gaaatcctcg gccgtatgga gggagaagac ggcggcccgt catcggatga tgatgaagac
     3361 ctgggcgagg gcgtctctga aacagacagt gtgatcatgc agctcgtcaa caaaatcgtc
     3421 aacgacgcct ataaccgccg cgcatccgac atccatgtcg aacccaatgt gggcaagaag
     3481 aatgttgaaa tccggttccg ggtcgacggc gactgcagtc tttatcaaac cgtgcctttc
     3541 agctaccgcg cggccatcat ttccagaata aaaatcatgt ccaatcttga tataacgatt
     3601 aagagacttc cgcaggacgg caaaattaaa ttccggcgca acacgggaga tgaaattgaa
     3661 ttacgtgtcg cgacgattcc cacccagggc ggtgtggaag atgtcgtcat gagaattctg
     3721 gccaagggcg aaacgatgcc cctgcaggac atgggccttt tgccccgcaa ttacaacgaa
     3781 ctcattaaca ttatttccaa gccgtacggc atgattctgg ttgtcgggcc gaccggttcc
     3841 ggtaaaacca cgacgctgca cgcggccctg catcatatta atcgcccgga cagaaaaatc
     3901 tggacagcgg aagatcccgt ggaaatcacc caatacggtt tgcgccaggt ccaggtgcag
     3961 ccgaagatcg gtttcgattt tgcggcggcc atgcgttcct ttctgcgcgc cgatccggat
     4021 gtcatcatgg tcggtgaaat gcgcgatttt gaaacagcca aaaccggcgt ggaagcctcc
     4081 ctcaccggcc acctggtttt cagcaccctg cacaccaaca gcgctccgga aaccatcgtc
     4141 cgtctgctgg atatgggcat cgatccgctg aatttcgccg acgcgttgct gggcatcctc
     4201 gcgcaacgtc tcgtgcgcac gctttgcaag tcctgtaagg aaccgtatca tgcttccaaa
     4261 gacgaatacg acgaaatcgc taaaagttac ggtgcggaag aattcgccaa actcaacatt
     4321 ccgtacaatg acgactttaa attatacaga gcaaaaggat gtcccaattg cgacggaacc
     4381 ggatataaag gacgaatggg tatccatgaa ctccttgtcg gttcggaaga tattaaaaga
     4441 atgatacaaa aacatgaaac cgtggaggtc atgcgggaaa aagccatcag cgaaggcatg
     4501 accacacttt tgcaggacgg gctgctgaag acgcttaaag gatttacaga cttcaagcag
     4561 gtacgccggg tttgtatcaa ataatccgct tgtccgcgga ttttctccca tcaaaaattt
     4621 ctcttcattc ggtgatctcc ttttttctta tttgtccgtc aacggatccc cggcatcgac
     4681 atttcgttaa acatctttaa tattttattc ataaaaagac gaaataatcg tgttttccca
     4741 taagcaataa ataacttatc taataataat atttaataaa atcataactt tgcaaaattt
     4801 aataagattt tttggcattc tggcacggtc ttttcattag attaagacaa agaaaaaaat
     4861 ttcctaaagg aggcaacaga tcatgaaaaa aacattagga acaatgatag tggcggcagc
     4921 ggttattctt ttcacagcaa ccttcagctt tgcggaatat gcggcagccg gagcagccaa
     4981 tttccccttc ttccagctgg gatgcctgat cgtcggcgga ctgatcatgg tttctttgaa
     5041 acgcaaatat gacaagatgt atgtcggtga agtggtcggc atcttcgccc tgtacaccat
     5101 tctgatggcg ctgttcacca acccggtcat cgaaacggtc aaaaccatcg tcagctaagc
     5161 attgctaaaa caacatctca cgggaaagga ggaatgaatc atgctggcaa ctcataaaat
     5221 acaccaaaaa gatttcaata atctcaaaca ggtctttatg ctctccgcgt ggggtttcac
     5281 catggtagtt gtttctttcc tgttcctgta tatcggacat ctggtcgacg gtctgctggg
     5341 aaccacaccg aattttatgc tggggttttt tattctggga cttttcctgt gcatgatgag
     5401 gctttatcag gaagcctgta ttcgaaggaa ggatgtctga tcattaaaaa catgatctgc
     5461 tatatcatat gaccctcctg caagacgcta tgttttcagg agggtttttc tgatcaaaat
     5521 caaaggaaat aatccaacca ggcggaataa ataaaaaacc agattatttt actgtattcc
     5581 ctcctgacct ttctccaatc cgaagacgat aattcccaga aattggccgg cgctttctca
     5641 tagcgcgtcg gaacaaaata aaaagaggcg tcgttaaaat tgaattcctt cgccgccatg
     5701 aatcgtatcc ttcgcatatg atagggagag cttacaaaaa ttgccgattt aaggccatag
     5761 gcggtcatga cctttttggc tttgatgatt tcgatatgcg tgtcttcata aaaacccgga
     5821 aaaggcgcat tgttttctga atcagcatcc ggcgcatgct tatccagaac aagctgccgc
     5881 gtcccgtcct gatcatgatt tctgtaggtt ttattatagg ctgaaataat cagataatcc
     5941 gccatgccgg aagcaaccag ttccctggcc gccttgtggc gcgccttgaa atccggtccc
     6001 agcaaaagaa tgacggcatc cgctttttta taatccgtgg cgtaaagcag aaaatacgga
     6061 gaagccagaa ccattccggc cgtcaacagc agaacgaaaa tgaaacatgc caatccctta
     6121 tgacgtctga tcataatcat ctcttcaatc gttttacaaa atgagcatga attgtctgaa
     6181 cggtttttca ggaaatgtcg ccgaaataat ctgctgaaaa aatgtagatt tccgtttcct
     6241 tatccttcca ggcatccggc ggcaatccgg ctttgtggca ggtctcctcc agaaatgttt
     6301 ctctgtccca tttatattcc gttgccacct gcggcagcaa caaacccgag tgatgccggc
     6361 ggatgatgta caaaccgtgc ctgccgactt caatgtcgtt gacatccgca atgcgcttga
     6421 gcggactcaa gacggatatt tcaaaggtca gttgttgaat ttcttccgct cttaaagacg
     6481 ggaatcgcgg atcatggaaa gccgcggcaa cggccatttc ctgcacggtt tcccagagcg
     6541 gtttgtatgc tttaatataa ccgatgcaac cgcgcaaccg cccctgcttc ttcagggtga
     6601 cgaatgcgcc tcttttcaac cgcagattgt tggagtcaat gttcaactcg ggagtttccc
     6661 gatgattgat tttagcggca atcgcatttt tggcgatgtc cagaagcgtg tttttttcct
     6721 gatctgtcag ttccatgttg ctttcctttc tccatcattc aatgacgcat acatttttcc
     6781 gtcgttacga aagaaaataa acaagatccc ttttaaggag aacggtaaaa aacggcggcg
     6841 gcgtatccga caacggattc cttatccccc gtgacgtctc cggaattggc gtatttcaaa
     6901 agacacggcc tgtcggcctt taacatgcgg gcaacagaca tcacaaccgc catgggtccg
     6961 cccccgcagg cttccacgtc accattttcc agactttgca aaagcccctg tgagtcgccg
     7021 tgtttcaaat ggcccagggc aacggcatcc attttctttg ctgcattgta gttataaaaa
     7081 tgcgacaaat cggagcttcc cacaatcagc agatttttat ctgccgatac gtcagctatc
     7141 gcttcagcca gctcccggca ggtggcggca tcctgactgc ccatgaccag cggtaaaaat
     7201 gaaaattggc ccagggcaac ctgcagaaag ggaagctgta tttccacggc atgctcctgc
     7261 agatgggctt gcggaatgtc cgtgacgatc ggactttttt tcttcagctt gtccgccaga
     7321 gcttcttcaa cgggaacgac acccaaaggc gtttcgtagc cgcccttgct gaaaatcgaa
     7381 atgccgcgga aagcaacccg gtggctgggt cctacgacca tcaccgcatc gtatgtcctg
     7441 ccgcggacca gtttgtaagc atgggccgcg acgtgtcccg aatacaagta gccggcatgc
     7501 ggggcgatca acccgacgat attgccgtca atcgtcattt cgggaacggc atcaaaatac
     7561 ttttcgatgt ccctgcgcaa aacggcagga tttcccggat accaggaacc ggctatcact
     7621 gatttcttga taggattcat ctctctattt aaaccgccag atgacacccc ggggggtgtc
     7681 caaaacatcc acgcccagct ccgccagacg ggccctgcag gaatccgctt ctttccagtc
     7741 ccgggctttt ctggccgcgt tgcgtttgtc gatcaattct tctatattcc ggtgaatcgt
     7801 cccgacctcg aaatccatga cccccagaac aatattgata ttttccagcg cttttaaaat
     7861 ctttaccgca ttattcttat tgatcaagcc tttcgtcaga ggctcgttaa tcttaccgat
     7921 gaaagaaaac aaagcggcca gcgctcccgc gatgttcaga tcgtcatcca acgcggcggc
     7981 aaactgctgc ttgagatcgt atatcaactg atcggtttct gcaaaaccgt tcccgccgac
     8041 accgtcatcg atcgcatgaa gccgtcgaat aaacgtatga atttttctga cggtattctt
     8101 ggccgtctgt aaagcccccg ccgaataatt gaccggtttc cggtaatgga tgctcagcag
     8161 aaaaagacgg atatcgctgc cgctgtaacc gtccttctgc aacccggaca gcgataaggc
     8221 attgttcagc gaacgggaca ttttgcgccc ctccaccatg accagttcga cgttcatcca
     8281 gtaattgggg ccttgatgtc cggaaaaagc cttctgaatg gctaaaacgt tttcgcagtg
     8341 aggaaacgtc tcatccgcgc cggaaacatg aatgtcatac gtgtctccca gatacttctg
     8401 agaaattgcc gcgcactcca gatgccagct cggtctgaca tttccccata ccgttttata
     8461 ataaatccct cttttgagct ctttcagact cgaccgcttc agcagggcaa aatccgccgg
     8521 gctgtctttt tcgtaatcat caacatcaac ggatttgacc tttctggttt tgccgagatc
     8581 gatattggac agggcgccgt aatccgcaag cttggatata ttgtaataca ccgaatgcag
     8641 cttttcatac gcgtactctt tatccgttag tttttcgacc atcttcaaca tcgcctccac
     8701 attttcagag gacttgacac agatattgtc ctgacgaatg ttgagaaatt tcgtatcctc
     8761 aatgaacgtc tgcaaatatt ttttcgagta ctccgcaagc gccacggatt ctttttccga
     8821 tcctctgatg gatttgtctc cgaaatcgac aatatccaga acatgcttca ccttgtatcc
     8881 cttgtagatc aaataacggt tgatcagatc ggataccaca aggcgccggt aattacccaa
     8941 atgaggcgta tcgtgtatgg tcggcccgca ggtccgcatg gccacttcat ccgttttaat
     9001 cggattaaaa agcgtttttt tgcgcgcaaa cgcattgaaa agataaaagg tggatattgc
     9061 tttttttacg gcaaaaagtt ccgtgcttaa atatctttct ccgccgtcgg ggaagatcac
     9121 aacgatcagt ccctcgtcca tctgccgggc ctgctgcaaa gccacgaaca gggctgcgcc
     9181 cgaactcatg cccacaaaga gaccttcctc ccgggccagc ctccgggaca tttcaaaggc
     9241 atcctcatcc tgaatgttga tcttctcatc cagcatgttt ttgttgaata tcccgggacg
     9301 ataggattcc cgcatatttt tcaacccctg tatcttatgc tggaggtagg gttcgacacc
     9361 gaccattttg atctgcggat tcagttcttt gagccttttg gaaatgccca tggccgtccc
     9421 gctggtaccg agactcgcca ccaccatggt cacttttccg tccgtctgat cccatatttc
     9481 ctgtccggtg ccgaaataat gagcggcaac attatcttca ttgttaaact gatccgtccc
     9541 gaaatattta tccggattct cgcgcagcat tttataggcc acttctatgg cgccgtcggt
     9601 gccgtggccg gccggcgtaa agtataattc ggcccccatc gcccgcagaa tcttttttct
     9661 ttcttcactg gccgattccg gcatgatcag gcacagttta tagcctttga cggctgcaat
     9721 catggccagg ccgataccgg tgttaccgct tgttgcttcc agaataattt tgtcgtgcgt
     9781 cagattacct ctggattccg cgctttcgat cataaacagg gcggttcggt ctttgatgga
     9841 tccgccggga ttggcgcttt ccattttagc cagaattctg acctttttat tgggattgag
     9901 cctggtaatt tccaccagcg gcgtattgcc gatcccttcc atgacatttt tatagaatgt
     9961 actcatacgt aaaaatacct taaaataaaa aaacccgcag gggttaaaaa ataatcagca
    10021 tctttgggaa tctttattca tttccggacg tgaatcaact atccattttg gccacgggaa
    10081 ttttaaaata ttgcaccccc tcttctttta aggtttcctc ctcccggggc gtgctggtac
    10141 ccttgatgtt tcttttttct tcatctccgt aatgaatctt cagcgccact tccgcaaatt
    10201 tatcgcccac atcttcgaaa tttttatcaa tatactgatg cagcattttc agagcgccca
    10261 tcccagacag ttccttgctc cgggatttat tttccgtccg ggattctttt cccatgatcg
    10321 taattgagga cggaacaatc tccacaccgg agctgttaca gatcgggcag gaaaccagtt
    10381 tttttgaatg ttgcagaaca aagttttccc gatctttgaa ccacccttca aacgcgtgtc
    10441 cgttttcaca cttcaaatcg tatataatca taataaataa cctgttttat gacccatttg
    10501 cattatttat aacatgcctg tttataaatt acaattacat atcaattctt tcgagcttta
    10561 ttcctttccg ttaaaatcat atttttgatg taaatgataa agaaaacgga aaaatgaaca
    10621 aaaaaagttg gacaattgca aataaatttt atatcgtccg ccgtgagtcg ggatgtggcc
    10681 cagtttggta gggcactgcg ttcgggacgc aggagtcgcg cgttcaaatc gcgccatccc
    10741 gaccattttt atgaagtccc gatttcaaaa gcgtatcccc tcaatagccc aaagggcacg
    10801 ataggcgtac tcaaaaatgt gttgatcgaa ttatcccgaa agtgaatgac ctgaataccc
    10861 tccagggcac catggggtcg ctataggcga cgcggtctct gaaacgtttg cgaaacaatc
    10921 ccgccggcaa agctcacagg atcgtctcca accgcaaacc gtcaggcgcc ctctgtggaa
    10981 aacgacaggc accatgcccg cgatggcatc ctgtgaggca ggagcttcgc atcaaccgac
    11041 aacggatgca agtttgaaga tcggcagata catcgccaca atcatgccgc cgacaacgcc
    11101 tccaagaaaa accatcatca ccggttccag aagcgccgtc atggcatcca ccgcagcatc
    11161 gacctcatcg tcatagaaat cggcaatttt ggacagcatt gaatccagcg cgcctgtcgc
    11221 ttcaccgacc gcaatcattt gcacaaccat gggagggaaa attcccgttt tggtcagcgg
    11281 ttcggcaatg gactgacctt cgctgatgcc ctggcgtgtt ttcatgagcg catcttcaat
    11341 aacgacattc ccggacgttt tgctgacgat ggacaaacct tccagaatgg gcacaccgga
    11401 gctcatcatc gtagacagcg tgcgcgcaaa tttggccacg gcaacttttt tcaagagcgg
    11461 cccgaaaacc ggcgacttga gaatcagcga atcaatcgtc caacgtccct tttccgttct
    11521 ataaaacctc gaaagaataa tgtaaattac taccagaatg gcaacgccaa ggtaccagta
    11581 actttgtgca aactgactcg cagcaatcag cgcggcggtc aatgccggca attccccgcc
    11641 cattccctca aacatttttt gaaaaacggg aataaccttg atcagcagca gggccacgac
    11701 cgcacaggag atcaccagaa cggcggccgg atacgtcatc gcacccttga cacgcgcttt
    11761 cagtttcatc gccttttcca tgtagctgga aagtctttgc agaatgatat cgagaatacc
    11821 gcccgcttca cctgcggcaa tgaggttgac aaaaagttca tcgaagattt cgggatattt
    11881 tttcaacgcg ttcgttaaac tcgttccgcc ctcgatgtct tctttgatcg acttgataat
    11941 tttggagaag gccttgtttt gctcctgctg ggcgagaaga tcgagacatt gtatcagcgg
    12001 aagaccggcg ttgatcatcg tggaaaaaat acggcagaag ataacgacgt ttttttcctt
    12061 aaccttcccc tgtaaaaagg gcaggtattc aagaagatct ttgggttttc tcttgacttc
    12121 aatggatttg taaccctggc ggcgtaaaag actgcgaacg gtaatttcat ctacggcttc
    12181 catttctcct tttttggctt catctttttt cgttaccgct tcccagataa atacaggcat
    12241 atttagtcct ttcccccaat cggataactt aaagttttcg cattttaaac cagatttttt
    12301 acaaattcaa caagttaaat aaccgtcttt cgtcgaaccg gcaggccatt ttccaaaatc
    12361 gcttattccc tgcgcacgat gcacgcatca aaaagacggt gacgatcaat tcatttgccg
    12421 ctttgtttag ttgaccgggt tcgctgacgg gatatttcat acaaaataat tcctgcggca
    12481 acagacacgt tcaacgaatc aaaattaccg gtcagcggga tcgtggtcaa aaagtcgcat
    12541 tttttcttta agagaggcct gatgcctttt gcttcgcctc ccatcaccag agcgacggga
    12601 cagtcaaagt cggtttccgt caatttcttc ccgccgtgcg tctccgcacc gaaaatccag
    12661 aacccccgat ctttcaggtg atccagcaat tgagccaggt tgacaacttt ggcgacgggg
    12721 atatgatgaa cgctcccggc ggaggctttg ttcacggccg ccgtcacccc tgccgatctg
    12781 tcttccggaa taatcacgcc gttggccccc cagcaatgag ccgtccggat tatcgaaccg
    12841 agattttgag gatccatgac gctgtccaga atgagaatca gatcgtgacc acacgcgggg
    12901 tgacgatttt tcatcacagt ttccagatcc gcgtaagcaa atgtttgacg aaaagcgatc
    12961 atgccctgat tgttgggtgt tccggccagt ttatccagtt ttttccgttc acaaaattcc
    13021 acgggaatgt tgtgagcttc cgccatagcg ataatgtcct gcacagcggg gccggtcctt
    13081 ccggaggcaa tcatgatttt ttcccatccg cttttctgca gccggaggag atctctgatg
    13141 gaatttatgc cgtaaatgaa ctccatggta tcttccgcga tcggaaaggc attaccgtgc
    13201 cgacagccct tcggcaattt cctggacaat ctggcggcag gctttcaagg gatcggctgc
    13261 cgtgcgaata ggcctgccga cgacaatata atcggcgccg gctgccacag cgtcatacgc
    13321 gctgagcgtt cttttctgat cgtcgtcttt tcggatttcg gccgcactgc gaatgcccgg
    13381 cgtgacaatg acaaaatcct cgccgaatgc cttcctcaac atgacaatat cctgcgctga
    13441 agccaccacg ccggatgctc cggccgcctt cgccaacccg gccaggtgaa caaccagttc
    13501 gtttgttgtt ttttgaaagc cgatttcttt caaatcgtcg ttattcagac tggtgagcac
    13561 cgtcaccgcc agcaggatcg gcctggcacg accgagtgca tcggaacacg tccatgaggc
    13621 ggaaaccgac tctcggatca tccgggaacc gcccgacgcg tgaacgttga acatgtccac
    13681 atgcattttg acggcggctt cggcagcacg ggcaacggtg ttaggaatat cgtgaaactt
    13741 cagatctaaa aatacttttt gatttctttc cttgatcgcc cggatgattt tcggcccggc
    13801 ggctgtgaaa agttctttgc cgatcttgaa cattccgaca tggccggaca acaggtccac
    13861 cagcgataac gcatcttcat aattctgaac atcaagcgcg aagattaatc tctcgcctgc
    13921 attcttactc gatatattct tcatcgccga taaactctgt gctgtccgga attggtttga
    13981 aatcctcttt ggttccgttc agatattcgg gattggcata aacaaccttg cctgcagcaa
    14041 tttcgcgcag cgcctgaacg atttctctgt ttttactcac gaccagaggc tgcgaactct
    14101 tcagcagctg atgcgcccgt ttggaggcca gggacaccag accgaaccgg gttttggcca
    14161 ctcgcaacga atcttcaatg gtaattctcg ccatagcgtt tacatctcct tcaagtatat
    14221 atttatttta taaattctct tatttctctg tttaatcttg tcctcttgca cttttccgcc
    14281 gtataaatgg caatcaattc attgacggcc tttttcagat cattgttaaa gatgacgtaa
    14341 tcataccagg aaatctcttt tatttcactt tccgactgct tatatctttt ttgaataacg
    14401 ttttcacgct catgacctcg ttttttcagc cttttcaata gttcctgacg ggacggcgga
    14461 agaataaaaa cataaatgcc tctccggaat tcctgtttta tttttttggc gccccgcggt
    14521 tcgatatcca gcagcagatc cctgccgtca cgcacacctt cttctatgaa gcgccgcgac
    14581 gatccgtaca ggtggccgta attttccacc cactcaatga actcgccctg cctgattttt
    14641 ttccgaaacg cctcacgaga aataaaatta taatcctttc cgtctatttc accgggacgc
    14701 ggcggtctcg acgtatacga aaccgaaaat tttatttccg gaaacgcttc cataaagccc
    14761 tgacagatgc tgctcttccc cgtgcctgac ggcgcggaaa cgacgatgaa taatcctttg
    14821 gacatagcct cactctatat tttgcgcctg ttcgcgtaat tttcccagtt cgcttttcac
    14881 gtcgatgaca tgacgcgtga tgtcgacatc gccgaccttg gatccgatgg tattgatctc
    14941 gcggttcatt tcctgcagca gaaaatcgat ttttctgccc tcggattcat cgctgtgcag
    15001 aagaacttca aattggccca gatgacttcg caggcgaacg atttcttccg taatgtcggt
    15061 cctttccgcc atgatggcca cttcctgagc caaacggttt tcatcaagcg caaacccttc
    15121 ggtcagttct ttaatgcgtt ccgagaggcg tttctgatat tccgaaacga cctgcggcgc
    15181 acgcacggca atatcattaa gaacaccgtc tatcattttc agcctcattt gcatatcctg
    15241 caacaggaca agcccttccc cctgccgcat cgcggccaga atttccaagg cctcgagaaa
    15301 cgatttttcc acctcgcaga gaaaatcaga gccgatttca gtttccgcgg gcggcgtaaa
    15361 aatatcgcga aaggcggtca atgtttttag cgtcacgggg gactccagat cgaattcttc
    15421 ccgaagtctg tttaatacat taaaataccc tcgcgctatt tccatattca gattcacttt
    15481 cgctgtgtcg gcaccttcgc cttcgaactt gatgaatact tcaatgcgtc cgcgcttgaa
    15541 tctttcggta attttctttt tatagtccat ctccatcggg aaaagtgccg acggcgtgcg
    15601 cagcacgatt tccagaaaac gatggttcac ggatttggct tcaacaatga tatttttgtc
    15661 ctgaaaaaat ttttccgccc gtccgtagcc ggtcatgctt ttaatcattt taataaacct
    15721 gtctttgttc gattattgtt cactattttt aatgatccgt cattttcaac tgcgtcaaat
    15781 atctgtttct gatccggtcc agcatctcca gctgttttgc atcggcataa tccggatagg
    15841 tccagggcag aacacagaat tttttcccct gataaacaag cgtcaaatcg gcataaattc
    15901 cgtctctcaa atatggacgg tgggtgtaac tcttgccggt cgctaaaatc agatgagcct
    15961 tggatatgta acccggatca atgtttatcc ttctcttcaa accggctgcc gattttattt
    16021 caatgtcatt ggtggccagt ttgatatccg gcagggattc cggatggatt aatttttcca
    16081 tggtcagata ccggcgaatc agattttcgc ccatttctcc gccgtagtaa tccgtgtaat
    16141 caaacggctg aagcgcactt ataaaatccg ggtccccgta agcccgggaa agctctttta
    16201 tcgtctcgtc tatgattacg gcatcctgcg caaaaaggct gaaaaataat ttcaccggtt
    16261 cggccggact cggtttactc ataatacgtc gatcagctcc tgtcgcgaaa cggtggccgt
    16321 tccgactttc tcgaccacaa tgccggcagc cagattcgcc agacacgtgg cttccttgag
    16381 gctggctttt gccgccaggc acagcgcaag gacaccgatc accgtatccc ccgcaccggt
    16441 cacatcatag acttctctgg cctgtgccgg aaagtgcgtg tgaacgattt tccggccatt
    16501 ctcaaacaag ctcacgcctt cttcaccccg tgtcaccaga agggccggaa acgaatgttt
    16561 cttcaaaatg atttcgctca atctctcaat attattggtc ccgttcagct ccataccgac
    16621 agcgcgctga acctcgtaat ggttgggagt gatgacatgt acgcccttat aaacggaaaa
    16681 attgctttgt ttcggatcca gacaaataaa aatccccgaa tgatcaacaa ttttttttat
    16741 gccctgaatg agttccttcg agacaacacc tttattgtaa tcggaaatga cgatcgctcc
    16801 tattttatcg acccgggacc gaacatagtc cagaatcttg tggatgctta ccggagaaat
    16861 cggtttcctg tcttccttgt caaaccgcac catctgctgt ccgtgagcca cgattcgcgt
    16921 tttcaatgtc gtcggacggt cttttttaac cacaatgccc ttcgtttcaa tcttacgatc
    16981 attcaactcg gccagcagct gtttgccgat cgtatcggcc ccgacaactc ccgaaacgta
    17041 cgcccggcct cccatggaat ggatgttgtt gagcacattc gtgcaaccgc cgagcaaaat
    17101 gttgtctttc tgaacgtcaa cgacggggac cggagcttcc ggcgaaatac gcgacacatt
    17161 cccccagatg aaatgatcga ccatgacatc gccgatcacc agaacgcccg cgcgggggaa
    17221 accggcgatg atttccaacg ctctcttttt tgtcagtaat ttttgcacgt ttgaaggcaa
    17281 ctcccgaaac catcatgctg aaaattaaaa aaagcgcttc acgcgcccgt acgtttacaa
    17341 agttgcgtga tattattatt aagatctgaa tttgtcaaac attttctaaa aaacaggacg
    17401 tcggctattt atgattatct tgacatgtcc ccggaatctt cattaatatg cctgcgggag
    17461 aaaaacgaaa cccgaaaagc cgttattcgg cgaatcgaat gggataagac catgttgaat
    17521 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaaacccga aaagccgtta
    17641 ttcggcgaat cgaatgggat aagaccatgt tgaatattaa acagttacgg tatggaacgg
    17701 acaatttctc ataccttctg tacggaaaaa cgcaagccgt ggccgtcgac gggggcgcat
    17761 ggaaagaaat acttgatttt ctgaagcaaa acaatctcat gctgaaaatc gtcaccaaca
    17821 cgcattcgca ctacgaccat accccgggaa acgaccattt gctgcaaaaa acaggcgccg
    17881 aattcattcg cgcggctgat ttccaggacc atcagcctct tgttatcaac gcagaacgca
    17941 tcctggtcta ccgaactccc ggacatacag acgattccgt ctgctttcat gccgggagtt
    18001 ttctcattac cggggacacg cttttcaacg gaaccgtggg caactgtttt tccggcaaca
    18061 tgaatatttt cttcctttcc atcaaacggc tgatggacct gtccggtgaa acgatgattt
    18121 atgccggcca cgactatatc ctgaatgctc tggcgttcgc ccgtcatctg gaaccccgga
    18181 ataaaaatat cgatcttttc cggaatgcct gccgctccag gaacacaacc tattccaccc
    18241 tggatgaaga gcgcggcatt aacccgtacc tgcggtttaa tgaagaatcg atcatccatc
    18301 ttcttcgcaa aaacaatctg cctcacgcca ccgagcagga acgctggaac tccctgatgt
    18361 ccctggaata aaaaatccgc cgggcattta atatttgaca gacgcaataa attattttat
    18421 attttatcat acttattcat tttcattccg gcattgttgc gcaacatctt cgaaaatcca
    18481 caaaggactc gagacggatg gacgaatgca ggaaataaaa acggcaaatg caaaaaaaac
    18541 acctccagta aaaagcagaa aagacacaaa agaagccttc ctttaccgac cccaaccgga
    18601 agaaaagcat gaaaactggc tccagaaatc ccttttcctt tttcagcaca accctgcttc
    18661 cattgttctg ttaagacttc ccgaaaaaaa atacatcgac atcaataaaa ccgttttgcg
    18721 gacgttcggt ttttcccggc aggacgtcat aggaaaaacc agcgacgaac ttgatatttt
    18781 tgtcgaacag gacaaacgac agatcgcact gaaacaactg atgcagcagg gttatattga
    18841 aaatttcgaa atgaagttca ggcgaaggga caagacgatc ctggacggtc ttttttccgc
    18901 ggaaatcatc caacatcgcg aaaaacgata tgtcctggtt gttattacgg atattacatc
    18961 ccggaatctg gcacagcagg cacaacgcga aaacgaagaa aaataccgga cgattctgga
    19021 aaccaccgag gaagggtatt atgaaataga tcttgccggc aatttcactt tttttaacga
    19081 cccggtctgt gcactgctgg gttattccga aaacgaatta aggggaatga acaaccgtca
    19141 atataccagc cgggaaaccg caagaaaagt tttccaggcc tttaacaagg tctatagaac
    19201 gggacgcccc acaaaagaat tcgactggga aatcatccgg aaggatggga caaagcgcta
    19261 catcgaacag tcggcgtccc tgaaaaagga ttcgtccgga aagccgatcg gcttccgcgg
    19321 aattattcac gacattaccg aacggaaaca aatggaagat gccctgcgaa aaagcgagga
    19381 aaatttccgt cattcccttg atgattctcc tctgggcgta cgcattgtca cggaggaagg
    19441 cgaaacgctc tatgccaatc aagccattct cgatatttac ggttacactt atgtggatga
    19501 attaaaaaac acgcctctgc acaaacgtta caccgcagag agttacgccg attttcaaca
    19561 gcggcaggaa aagcggttaa tgggggaagc cggtccgccc gaatacgaaa tcagcattgt
    19621 cagaaaagac ggtgaagtgc ggcatgtcct tgcactgcgc aaggaaatat tctggaacgg
    19681 gcaaaaacga tatcaaatca tttatcaaga cattaccgaa cgaagaaagg ccgagttgaa
    19741 actgcaggag acccttcata cgctgcgcaa atccattaat acgaccattc aggtgctggg
    19801 catcgcttcg gaagcaagag atccgtatac cgccggacat caacggagag tggccgatct
    19861 ggcccgcgct atcgcgacgg agatgaaact gccgcaagat accatcgaag gaatccgcat
    19921 ggccggagcg atccacgata tcggtaagat ttccatcccg tcggaaatat tatgcaaacc
    19981 aaccacgttg agcgaactgg aattttcttt gataaaatcg cattgccagt acagttacga
    20041 aatcatcaaa gacgtcgaat ccccctggcc gcttgccgac attgtatacc agcatcatga
    20101 acggatggac ggttccgggt atccccggag tctcaaaggc gaggatattc tgattgaagc
    20161 gcgcattatt gccgtcgccg atgtcgtgga agcgatcatt tcctaccgtc cgtaccggcc
    20221 ggcgctgggc atagagatcg ctctggctga aatcgaaaac aatgccggta cgctctatga
    20281 cgttgaagcg gtgtcagcct gcctgaaact gttcaggaac aagaatttta aaatctgaac
    20341 gccctgattt ttttattctc tacctccgat acgtttgaag acgacttcat cattcttgaa
    20401 acttttttca tgattgactt cactattatt atcaagtaaa aatttattca ttaatccgtc
    20461 tgagcaggaa aacgtttatg gtccattcag cgttactggt cacattaggt gttgccataa
    20521 cggcgttcat gtcgttctgg tcggtggtgt tttccgtctt tcccgccgct gacaataaaa
    20581 ttcacaaggt ggcaacattg tgggcaaagc tattactgat gatctgcaac acaaaagtcg
    20641 atgtcatcgg taaagaaaac attctgttcg gaaaaccgca gatttttatg gccaatcatc
    20701 aaagcgattt tgatatttta atttcactgg cgcatattcc ggtgcaattc cgctggatcg
    20761 caaaaaagga attgttcgcc attccggtat tcggcgcagc catgcgcagc gccggatata
    20821 ttgaaatcga ccggagcaac cgcgaaaaag caatgcaaag cattgatgaa gccgcactgc
    20881 gcatccggag cggcaagtcc gtcatgacct ttccggaagg cacgagaagc cgcgaaggag
    20941 aaatcaaagc gttcaaacag ggcgcctttc atctggccgt caaatcggga gtgccgatca
    21001 ttccgatttc cattatcggc agcggccgga tcatgcccaa gcggtccctc cggataaaac
    21061 ccggtcagtt gaaaatggtg attggaaaac ccatcgacgt tcaggacgtc acgattgaaa
    21121 acagacactc actgatagaa aaagtcagaa atgaaataat aaaaaattac aatgcctggc
    21181 aggatattga aatgtctcct ctgaaagatc tggaaagcgg ggcacattga atctgcttac
    21241 cgttatcgtt cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntcctctgaa
    21361 agatctggaa agcggggcac attgaatctg cttaccgtta tcgttcccgg catcgttcag
    21421 gaatttaccg aactgtttcc tgtcagcagg tttgagcacc tggttattgt acagagcttt
    21481 ttcccgggat ttcatcagcc gggcggggcc ttcgacgcgg tgcttttatt gattatcgcc
    21541 gcaataccgg ttgccgttat cgggtatctg ttcaaagacc agatgcacaa catctttgaa
    21601 tcagcccggg cagcggcatt tttcttaatc atcaccggca ttcttttgtt cttagcagac
    21661 aagatgaaga agacgcgacg ggatatcaaa aatatgaccg tcatcgacgg tatcctctcc
    21721 ggaagcgccc gtgccgtccc ttttcttacg ggcatttccc gctcggggac aacgatcacg
    21781 tccggaattt tctgcaatct gaaccggaac gcggcggcac gcttttcttt tctgctctcc
    21841 ctgccggcgg tatgcggggc ggtgattctg aaagccggct atctacggca gatttcctcc
    21901 ccgggatcat atctctactt cgcagggttc gtttgtgcgg cattggccgg actcctcgcc
    21961 ctgaaactgt ttttcctgat tatccgggag gcgcgtttaa aatactttgc ctattatggc
    22021 tggttcttcg gaacgtttac gctgcttgta aaatcttctc ttttctgaaa taatatcggt
    22081 attactatta ttgattcggc cacaacatgg aaaaaaaaca gacggacaat aaaagaacaa
    22141 gagaaatcaa aggcgcggtt tgtctggcgc tgggcctctt tttgctcctg tgcctggttt
    22201 cctatcaccc gcaggacccc tctttcacac attttgtcgc gggcgatcac accacgcata
    22261 attttaccgg caaggccgga tcgtataccg cagattcact gatccgcctg ctgggcgccg
    22321 cttcgttgct tttccctctg atgctgttcg tttgcagttt taaatatttt cggtcttccg
    22381 actttcgcat cggcaccggc cgtttaatcg gctttctgtt tttcatcttt tccttcgccg
    22441 gcatcatggc gcttctgatc agcggaagca tctcccttta cggagaatcg ttgaaaaacg
    22501 gtaccggagg cctcacaggc gtcgccattg tcggtttgct gcgcaactat ttcaatgtgg
    22561 cgggaaccta tattatttta tttttgattt tcgtcgtgtc actgactttc ctggtcgatt
    22621 tttcgatgat ctcgttcacg gaaaaaattt ccaaatccgg cgccgccttt ttgttaagat
    22681 ggaaagagcg catcatggcc tttgtcgatt tcgtcttcaa ccgcatgaaa tccgaacccc
    22741 cgtccccgcc gcaacccgct attgtcgaaa aagcgccgga cgtgaccaaa aaagcgccgg
    22801 ccaagaaagt tgaacagaca catttcgatt ttacgaaggt aaaaagcgac gataaatttc
    22861 agttgccgcc attcaccctg ctggaggagg ccccgcaaaa ggatgcccgc gtcaaacgcg
    22921 atgcgctggt caccaattcc cggattctgg aaaagaaact gtccgatttc ggcgtcgagg
    22981 gacgggtggt ggaagtcacg cccggaccgg tcatcaccat gtatgagctg gaacccgctc
    23041 cgggagtgaa aatcaacaag atcgtcaacc tcgcagacga tctggcgctg gcgctgatgg
    23101 ctccgagcat ccgcattctg gccccgattc cgggcaaatc ggtcatcggt attgagattc
    23161 caaatttaaa aagagaatct gttttcctca aagaggtcat cgacaacgat tccttcacgc
    23221 aatcatcttt ccggctgccg atcgcgctgg gcgttaattt cgtcgggacg cccgtgattg
    23281 ccgacctgac caagatgcct catctgctca tcgccgggac caccggctcc gggaaaagcg
    23341 tcgcgcttaa cgccatgatc tgcagcatcc ttttcaaggc ccaaccggaa gatgtaaaat
    23401 ttctgatgat cgatcccaaa cggctggagc tatcctcgta cgaaggtatt tcgcacctga
    23461 tgcatccggt ggtgacggac ccgaaaaagg cgtcgcaggt gttgcgctgg acggtggaag
    23521 aaatggaaag acgttacaag gtcctcaatg atttgaaagc aaaaagcatc gatgcctata
    23581 atgagctgct cctcaaaggg ctgaaaagca aaacggtgct ggccatgccg aaacaaatcg
    23641 accccgaact ggaatccgga caaaccatcg atgaaacggc ccgggaagaa caggaaatca
    23701 agcacacaaa actgccttat atcatcgtcg tgatcgatga actggccgat ttgatgatgg
    23761 ttgcgccaag ggatgtggaa gaatcgctga cccggctggc gcagatggcg cgggccgccg
    23821 gaattcatct gatcatcgcc acgcagcggc cttccgtgga cgtcattacc ggcctgatta
    23881 aagccaattt tcccacgcgc atttcgtttc aggtgtcctc caaaatcgat tcgcgtacga
    23941 ttattgatca gcagggggcg gaaaagctgc tgggatccgg cgacatgctt tttattcctc
    24001 ccgggacctc caaactctcc cgcattcacg gcgcatacgt ttcggacaag gagatttccc
    24061 gcatcgtcga ctttatcaaa atgcaggcac agcccgcata cgacgattcc atcgaaaaat
    24121 atgccctggc ggccgcgcag atccagcatg aaggcgacga tgatttcgac gcaaaatacg
    24181 atgaagccgt ggcactggtc agcgaactgg gacaggcctc catttcactg gtccagcgtt
    24241 acatgaaaat agggtacaac cgcgccgccc gcattattga aaaaatggaa tccgaaggca
    24301 tcgtcggtcc ttccgacggc gccaaaccca gaaaagttct gattagaaag cttcccggtt
    24361 gaaaaacgca gagaaaacaa aaatcaatat tatcagtctg ggctgtccga aaaatctgat
    24421 tgattcggaa gtcatgggag gatttctccg ccagtccggc catgccctgg ttgacaaaca
    24481 taccgacgcg gatctcgtca tcatcaatac ctgcgctttt attaatccgg ccaaagaaga
    24541 agccattgat gaaatcctga ccgtcgccga agcaaaaaaa aaccgcccga acctgcggat
    24601 catcgtggcg ggatgtctcg cacaacgcta cggcagggaa cttctggatc agataccgga
    24661 ggcggatcgg gtcatcggaa ccggcgaggt cggcaacatt gtcaggcacg tcacagaact
    24721 catccggtgc aaaaaccgcc gtaaggctgt gatcacccgg ccggtttatt taatgaactg
    24781 caaccaccgg cgtattttac cggcatcggc attcagtgcc taccttaaga tatctgaagg
    24841 atgctccaac cgctgttctt actgtgtcat cccttccctg cgcggcgcgg cgcgcagccg
    24901 tccgccggat gatattctcc gcgaagccga acgactggcc caaagaggca tcagggaaat
    24961 catcattacg gcgcaggaca cgaccggtta cggctggaat ttgaaagggc ggcccaggct
    25021 ttccgatctg ttgaacgatc tgacgcaaat taaggggatc gaatggatcc ggctgcttta
    25081 tgcgcatccg gcccgtatta ccgccgattt gctcagcacg atcgctgaaa acgataaaat
    25141 atgttcctac atcgatctgc ccattcagca tattgacgat gccatcctca acgccatgaa
    25201 gcgcaaagtg aacgctgcgg aaatcaggca ggtaattgct caaatccggc atatcattcc
    25261 cgatgtcgcc ctgagaacat ccctgattac cgggttcccc ggcgagacac cggcccggtt
    25321 caaccggatg ctggattttg tccgacaaac ccggttcgat catctcggtg ttttcaccta
    25381 ttccagagaa gaaggcacag aagccgccgg cttgcgatat catgtgccgg aaaaggaaaa
    25441 agaacggcgc cgtgaaacga tcatgcatga ccagatggaa atttccgctt ccatcaacac
    25501 gtcgctcatc ggttccgtcc aggaggtgat cattgaaggg aaaagcgacc gcccggatta
    25561 tccttatatc ggcagatgcc gccgccaagc gcccgagatc gacggggtta cctatatcaa
    25621 ggggccttcg ccggacatcg gtcatatcgt caaatgccgg ataaccgcag cggacgacta
    25681 tgaccttttt ggaaagatca tccggaagtc ttcctagccc ctggatttta ttcttccttt
    25741 ttttttctgt ttttccactg cggacataat tcggacgatc cgggctgata gtcacggcag
    25801 attaacggcc ttgtttcgta tatggagcag agccagacct tgcctgtatt gtaaaggaag
    25861 ggacattccc ccggcgcatt gcccgtatcc gaagatatca ttctgtcgcc cgcccagaac
    25921 aaatggcgat ggtcgatcat ctccagaatg tcctgcctgt tttcggctcg ccagcggtca
    25981 taatcttcct gctgcgcata agccgtaaaa tcgacgaaac aacattttcc gcattgcaga
    26041 caggagagtg attttttcat caaaaccgtt ttgttccttt tcaccggatt aaatattttc
    26101 agaagatttg ccgtcatctt tcaatacttt aaaattatct cacatttccg gatgaattca
    26161 tagcccgtcg gtttctcttc cgaccgataa gcgcatgatg cgcaagaaat gctgaattta
    26221 ccctttgaaa tacccctata attgttgtat gaattttgac atagaacacg tcggggcaac
    26281 ccagggagca gaacgcgcat gacgacatct ccagaccgga aagaaactga acaagccggc
    26341 gtctccttca atacaaagca gaccgaaacg acatccgtgc ggccggaact tgccgaaatg
    26401 tttgcacggc agaaatttgg tctggatcaa aacagacccg aggcggtgga aaaacgcaga
    26461 cagaaaaatc aacgaacagc ccgggcaaat gttgaggatc tgtgcgacgc cggaagtttt
    26521 atggaatacg gagcgctggc gatggcggcg cagcgcggca gacgttccga agaggatctc
    26581 atggccagga cacccgccga tggactgatt gcgggcatcg gaacaatcaa cggcactttc
    26641 ttcaaagaag aaaaggcccg ctgcatggtc atggcctacg attacacggt tctggccggc
    26701 acgcagggat ttttcaatca caaaaaaatg gaccggatgc tcaaacttgc ccatgaacag
    26761 tctgtcccgc tggtcctgtt cgcggaaggc ggcggcggaa ggccgggaga cgtcgacgcc
    26821 atcggtgtga tgaccgccgg cctggatttg tcaacgtttg ccatgttcgc cggactcagc
    26881 ggcaaagtgc cgcttgtcgg gatcgtgtcc ggcccctgtt ttgccgggaa tgcggcgctg
    26941 ctgggctgct gcgatgtcat cattgcagcg aaaaattcca atatcggcat gggcggcccg
    27001 gtgatgatcg aaggcggcgg cctaggaaat tttaaaccgg aacagatcgg gcctattgac
    27061 gtccagacac aaaacggcgt ggtagatatc gaagttgccg atgacgtgga agccgttgcc
    27121 gcagcaaaaa aatatctctc ctattttcag gggacaacgc cgtcatggga agcggcggat
    27181 ccgcttcagc tgcgcgacct cattcccgaa aacaacaggc gcgcctacaa catgcgaacc
    27241 gtcattaaag cgctcgccga tacggattca tatctggaac tgcgccctaa attcggcgcg
    27301 gggatgatta cgggattgat tcgcatcgaa gggcggcctt tcggattgat cgccaacaac
    27361 tgcctgcata tggccggcgc cattgaagcc gagggcgcgg acaaaacggt gcggctgatg
    27421 cagctttgtc aggcacacgg cctgccgatt ctgtctctgt gtgacactcc cggatttatg
    27481 gtgggtccgg aaatcgaaaa acgcgcgcag gtgcgccatg tctgccggat gtttgttgtc
    27541 ggctcgcacc tgaccgttcc atattttacg gttgtcctgc gccgcggtta cggcctgggc
    27601 gccatggcga tggccaaagg aggtttccat gaatccttct tcacggcttc ctggcccacg
    27661 ggagaattcg gtgccatggg cattgagggg gccatcaagg cggggtttaa aaaagagctg
    27721 gcggccatcg aagatccgga tcagcgtgag cagctatacc gggacctggt ggcccaactt
    27781 tacaagcggg gcaaggccgt caacatcgca tcctacctgg aaatcgatgc cgtgatcgat
    27841 ccgtccgaga cccggaagtg gatcatgcgg ggcataaaat ccgcagcaac gggaaaaaaa
    27901 cagagccccg ttcataatta tgtcgatccc tggtaaccac aacaaggcgg attttacgat
    27961 gcaaattcaa gaaatttttt ccatctacaa agaggatctt caacaggtcg aaaaatgcat
    28021 cagtgacaat gcaatctctg aaataaaatt aattcccgaa gtggctcatc atctgatcga
    28081 cagcggcggg aagcgattcc gtcccctttt gctgctgatc gcgtcccggc tttgcggcta
    28141 tcacggggaa caccgttatg ccatggggac cgtcatggaa tacattcata cggcaacact
    28201 cctgcatgac gatgtcattg atcaggccct gatcagacgc ggcaaaacgt ccgccaataa
    28261 agtattcgga aattccgtca gtattctggt gggcgatttt ctgtgtttca aggcctttat
    28321 gctgatgagc gaaatcggca acaacaatat ttttgaactg gtctcccgga tgggaaatat
    28381 catgtcggaa ggcgaagttt tccaactgac caaatgcggg gatatcaaca tgacgcggga
    28441 tgactatctc aacattattg aaaagaagac ggccgttctg atttcggcat cctgcgccgc
    28501 gggagcgatc ctgggctcgg catccccgga aaagatcgac gccatggccc ggttcgggaa
    28561 aaacgcgggc atggcttttc aaattacgga cgatattctg gattacgcgg ccaaaggcca
    28621 gacattcggc aaatcgatcg gcaaggatct ggaggaaggc aaaatcaccc tgccgctcat
    28681 ctttgccctc aaacaaagca cggacgaaga aaaaaacacc atgaaagaga tcctctcccg
    28741 aaaaaaaagc tccaggaaag ccgctctgca ggtccggaca ttcattgaaa aatacgaagg
    28801 gctggaattg tcgcggtata cggcggcggg atacatcaaa gaggccaaac ggcaactcga
    28861 cgtttttgaa gattgcgccg aaaaaaaaca cctgattacc gttgccgatt acattctctc
    28921 ccgaaacgta taaactctat attttatatt cagcgcgccg aatgacttcc agctgcaaat
    28981 cacgctgcaa ccttgtgaaa taggccacga acccggaaat tctgaccatc aaatcgctgt
    29041 aatattccgg atgggccatc gcatctttca gcgtctccgt ggaaatcaca ttgaactgcg
    29101 tctgcatgcc tcccatcctg aaatacgtat ccgcgtaatt ggccatgatt tcaataatcc
    29161 tttcatgact gtccgaggaa gcgggaacaa ttttgacatt gaaggcgatg ttattatcca
    29221 gcgtttcggg tttcaatttc gccacgtccc ggagattatc cagcagattc ggcgatgccg
    29281 tcggatgcgg tgtgagtccg ggggtaaaag gttcgccggc caaccggccc gacggggagg
    29341 ctcccgtgac gcgcccgtaa accgtatgat tggccatgga ccaccaaccg gtcgcgtggg
    29401 gtcctcctcg ataattttgg tgagaggcaa aataatcatg caccaatgac gtcacccggt
    29461 tggccattgt tacggattct tcattgcccg aaccgaaacg cggcactctg gttttaatca
    29521 tgacatgcaa ccgggggtga tgggcgaaat tgctgtcaat cgcctcttta agttcggcaa
    29581 acgtgatttt tttctcatca tagaccagtg ttttgatcgc catcagagaa tcgacaacat
    29641 ccgccattcc gataacggat acgccggacg aattatatct ggctccgccc cgggttaccc
    29701 ctctgccgct ttcaatacac ccttcggtca tgctggaaat cagcggcgcg ggctgatgct
    29761 gttgatagat ctcgcccaat tggttattgc cgataatgga ttgctcgcac agaaattcac
    29821 actgttcttt gaacgctttc cagaaatcgt caaaggtctc gaaatcgccc tgcccgatga
    29881 tgcctgtgtc cggaccaagt ttccagttca tcaggggatg ttttccgtta ttcagcgcca
    29941 tttccagtac ggcgaccaga ttgaattcta tgctggacgt ggcgctgcag tgcttgccgg
    30001 gaatgcccgg ttccacgcag ccgttgacaa cccaatccct cacatcctca acggcccagc
    30061 cgcgcgccgt caaggattcc atgacggcgt catcgccgtg gatgcagggc gtcgcgcctg
    30121 tgatgtaatt cacatcgcaa acccttttca gataggcaaa actgttttta tccggtttat
    30181 accgggcatg aacattggga ttattcaacg aaagcaattc cgtcaccttg agaaggatat
    30241 atgtcatgtc gttgaccgca tcttccccct gaggcgtcac gcctcccagc gtgatcgtgc
    30301 cgttgggcga cgagccgccg ttctgccagt tgccgatatc cggagcaaga atctgatgac
    30361 tggattctat aaagtaaaga catccgagaa tatcgatcac ctgtttgatg tatgcttccc
    30421 gtttttgcgg ggtggtgagt ttatgcatat ccgattcaaa atacggctga agaatctgat
    30481 ccagccttcc gtacatggga ccgtcgtcgg tgctttccat ccccagacaa aggtgcatca
    30541 gaataatatt ctgaacggcc tcatgaagcg tttcagatgg actttccgga attttgagca
    30601 gcatccgatg catggtttcc aattcggctt tacgctgcgg gttcgttgcc gttttggact
    30661 cttcaagcgc ctgacgggca agattgcggg cgtaggcgtt gacaccctcc agactgatga
    30721 tcatcgcctt taacgtattt ttcttttcat cgtcggcatg agcatctctg gcaagttctt
    30781 cttctatctt tctgatcaac cctccggtcc ccagcttgat aagcgcttcg tgtcccgggg
    30841 gcacttccga catggatatc gttttccaat aataaacgga aaaaaacctg tcatgaatct
    30901 gaacgggcag accgccgttt gtccggcttc tccacaattc gtgaatattt ctttttgccc
    30961 aatagggaaa tacgtgcctg tgcagaatat gaatggattc ctcgctgatc tcatacggca
    31021 tgagctccct ttttgaaaag gaccgcagct caccccagct gtaacagccg atggagaaag
    31081 gatgtccgac gcaggtggaa acgggagatg tggtgtacgt tccggccagc agatcgtttg
    31141 tcctgatcac cggcgttctg ttctccagga tatacttcag gctttccgct ttacgcaggt
    31201 tgggatccca gggtttgccg tccttatcca cctcgtatcc gttggccaga tggaaatctg
    31261 tgatcagttt tccgtattcg tgacagacca ccgcttcctt ttttttaccg aaatactcgg
    31321 cgcgaaattg ttccagcctc ggaaaatcct ccagccctaa gccggccaga tgcgggtcct
    31381 ccagatattt gacgccggga tcaacttttc cgcccgctac gcggctgatt tttcttctct
    31441 gacgttcctg cctgcagcaa tcggtgtcgg ccacatcccg gccgacggat ttgtttgcct
    31501 ttctatgttc ttcaatctgt cggtccacgg ctttctgctg tcccttgtca aaaacaagcg
    31561 ccatcagata attgaacagg gaggcgagca tgatgttgcc ttgtgttcga atggtgccga
    31621 tcagcatcat tttatacatt tcatcggggg ttgcatcggt catatccaga agatgatcag
    31681 aggttttaaa gataatgacc gtttccacat tttcaggaat tgtcttcagc acacggacca
    31741 cgccatcctg aaacacgatc gccgcttcga tggcgctgtc ctccgatctc atccccagac
    31801 aaaggttgat ccagccctgc gtgcccttca actcctcgag cagcgcttcg gaagtattga
    31861 atcggccggc gataaattgc agaaaccctt ccagaagcaa ctgatgcttg cgcagcactt
    31921 taccgatgcc gacctttgat gcgcttaccg ccagacccca atagcctgat cgccgatttt
    31981 gatctgaact gttttccagc gtgttttgca tggctttttc tcttgcctga catcccttgc
    32041 agaagcgtca gttttttgtc tttaatttaa cgctattaaa tttaaaagtc aatcgttttc
    32101 cgtcaggatt cagaaaccga tgatagcgat aacgtatcga aaaagaatca tccgccgcca
    32161 ccgcaacagc ggaccggatt ggcaggaaag gccgttgagc cgaagtcaaa tcttgcgtct
    32221 gatatttcta tgagatcacc ggtatttcct cctgatgtag gcagtcagta aatcatcgac
    32281 gatacgatgc attgcacgtt cgggattttt aacgatcacc tttaaaatac cgctgttgta
    32341 aagagagaca atgccatgca gttcgctaaa ggtcctgata aaataaaact ccacgtcccg
    32401 gacatccggc tcagaagatg aggacagaac gttcttgccg atactcatga ccagattaag
    32461 atttctcgcc gagctttcat attcctcgcg ggcaagagat tcttcggatg ttccataata
    32521 gtcggatacc tgaggcaccc ggcgattaaa catgatgtcg taataattcg gatatttttt
    32581 agcaaacgtc aaaaatgttt tgattatttt tttcagtttg gccatcggct ccgcttcttt
    32641 ccggacagcc ttcgaaagca tggcatataa caactcaaat gtttttttat ggattgcgat
    32701 gatcagttga tctttgtttc tgaaataatt gtagaggttg gccgccgtca tattggttcg
    32761 tctgcccagt tggtacatgg aaagcttctc aaatccttct tctctcagaa tcgtcagggc
    32821 ttcttcgaga atggcgtttc gtacctcttc tatttctttt tcgctgcgtg tgattttgga
    32881 caatgcaatt cccgctctag acaaaattct ccacgtattt tttgaacatc ttcctcacgg
    32941 ttggattttt ttcccattga tataatcttt caaatgttcc cggaagcgaa cttgtggaat
    33001 acgcaagccc cttgtacagc tcaaagtagg cactcgcccc cagttgaaat ccaatgccgg
    33061 caaaagcatc caccatgcgg tctttgaagc tttgcgcccc ttttgacgaa tggacactcc
    33121 aggatttctt cgccatacca aaagcaatat gcaaagcgct gatgtaggat cctatttttt
    33181 ccatggtgtc gtacagcatg taaaaagtca ccagcggact gttgaacagg gcatccggta
    33241 tctttcggtt gctctccgtc aaatcggcaa tggttaacga acagaaataa tccattgaac
    33301 cgggattgcc gtagatcagc tcatgcgtct ccaggccttt aagaaaatca agcgagcccg
    33361 gcagattgct caggcaggtc ggctgaatgc cgattttatt cttcttgcag aattcaacaa
    33421 ttttcctgcc ggaatgatcc tctaaagaat gaaaataatc gtagggcatt ccgaacatga
    33481 acgcaccatg aacggaaata ccgtgatcct gaattttttt aattcgcgat gcgtaatact
    33541 cttcgacgga tattttctct tttttgattt tggctacgat attcttgccg acatactcca
    33601 gattcctgat atccagcgat tccaatccga taaagaaatg cgatgcgccg gaaagcctca
    33661 gtttttgaag gagcttgtca ctatcggcga cctcaatgct gatttgcgcc gcgtaattga
    33721 tccgtaaagg actttcgatc attttgtcca gaacatcgtg cagctttttc cccccgaaca
    33781 aaaggttatc gggcgagatg aaatagaacc ggttcttaaa cgtgacacct ttcttctgct
    33841 tatccagcag ttcacaggta aaatcctcgg gtgaacgaga tgtgaacttt cttcgctctt
    33901 ccgggaacga agatatggaa cagaaactgc aggaaaacgg acagcctaaa aagggagtcg
    33961 tcacgttcgt ttcaatgatt cgtgaaagga gcgggccgat gacgggaaat ttattaataa
    34021 agtatggttt gattttaggt aattcgatga cccggtcaca tccccagacg ccatcctcaa
    34081 tcgggatatt tccgtgatac tcttttttca gattcgaacg cgcaagatcc gacataacgt
    34141 ttttaatggt cggcaaatcg ccggcgccga taacctgcgt aacgatttgg ggatgcggca
    34201 gatactttct cagatacatg tccacatcat taaagctggt tgaaacatgg atgccgccga
    34261 tgacgaccgg tatgttgact ttgttcaaaa tcagaatcac ggcacaagcg gtgggaaacg
    34321 ttgaactgat cagagacatg aagactgcca ccggtttttg tttttcttcc gcaatgatct
    34381 gtccgatcgt tttccgggta tcatcggcga tgatgactct tgcgtcttga tccgcccggc
    34441 aaatctgatt ggcaatatag taggctttat agggctcgat ggccatctgc tcgagcatcc
    34501 ctttccggag caggttggta ttgaagctca ggaattcggt ataattcgtt tcattgcacg
    34561 cttccagctt tctgcccgtc aggtattcaa ccgtatcttc aacggtgaag cccgccacgc
    34621 ctatttttcc catcagatcc acggtcgtgg gatcgtacat aatataatat tttttcaaat
    34681 tcttttttgt gtcacgattt tcagcgaatg attttgtcat ttttcagttc tcacgaaaca
    34741 atttaatgag gcatcttcaa tggcagaaac tttactcaag gtagaagttc ttttctctga
    34801 acaacttcag acaggcgtcc acgacagcgc tatcatagag aatccccctg tttttttcga
    34861 tttcttccag agcggctccg attcccagcg acggacggta tggacgatgg gacgccatgg
    34921 attccacaac atcggaaaca gccagaatac gggcttcaag gagtatttca tcccctttaa
    34981 gctgtctggg atatcccgaa ccgttcatcc tttcatgatg ctgaaaaatg atttccgcca
    35041 gcggccattg cgattccaca tttttcagca tttcatatcc gcaggccgag tgctccttaa
    35101 tgagggaata ttccagattg gtcaattttg tcggttttgt cagtatctcc gccggtatgg
    35161 acaattttcc gatatcatga atggaactgg ccatttgaat accgtcaatt ttttcctggg
    35221 gcaaccccat ttccgctgca atcgcacagg ccagattgga ggatctgatc tgatggcctg
    35281 ctgtatacgg atcccgtgcc tccacggcgg acaccatgac ctgaatggtt actcccatgg
    35341 atttcttgag actgtccaga gcttcctgca gtttctcttt cgcctgctca tgaccggcaa
    35401 cctcctgcaa aagcgcctga ttaaggtttt tataatcttc cgtacgctgt ttgacccggt
    35461 cttccagttc ctcattgatc ctggagactt tttccctctc ctcttccacc tgctggttca
    35521 tgagaaagtc gcgccgggca taaaattcaa tcgcgtagca ggccatcatt ccgaccagat
    35581 tggcgctgat gaagaagaaa ttattgccca ggaaaacatc cagcggcgtc gggttgagcc
    35641 cgacggtaac ggtttcataa aagataacct gcagccatcc ggcaacagac gcccaggtaa
    35701 aaaagagccg gatgagggtg tatccccaca tgaaaacgag cataaggccg gcgtagtatg
    35761 aatagctgac cggcggcggt gcaacaacga tcatgccgat gatgccggca ccggccagaa
    35821 aataaaccag tgcaagtatg ggctgtatgt atttttcaaa tatgggcaaa aaagatatcg
    35881 ccagcacgcc caccagcacc ggaccgatga caatgaaacg aatgatccag agcgcaacga
    35941 cttgctgcgg catcaggaga acatccagaa caccgaacgc cgcataaagc atcgctccca
    36001 gaatcatcat catgcggatg tgcggcagag acgacgtata atactctttc agaaacggcg
    36061 cttcgagata agacaattcc ccggaaaatt tgagggtcag ccgatttatt tgcattggaa
    36121 gattcatagc ctatcgtaaa acgccctgtt cgattttaca acatttatta aacgccgtcg
    36181 ggaagggcat attgatcctg aagccttccg gacctgtctt ctttcagcgc ctgaggcagc
    36241 gcattcataa tatcctgcgc cgtcatgccg tccgttcctt tttttaacgc cgccagatct
    36301 ccggcgaccc cgtgaaggaa tacccccttg cgggttgctt catcgaagga caaccccatg
    36361 ccgaacatcg ccgcaatgca gccggtcagc acatcaccgg aaccggccgt cgccattccc
    36421 gcattaccgc ttaaattaac gtaaacattc ccgtcacggg tgccgataag cgaatgcggt
    36481 cctttgagca cgatacaggc gttcaatgtt tgcaccgtct cccgcaatac ttttatttta
    36541 tcggcatgga tttccttcaa tgatttgccc gtcagcctcg ccatttctcc catatgcggt
    36601 gtcagaatcg ttgccgcttt ccgccggcga atgctgtccg gacagcctgc cacagcggta
    36661 agaccatcgc cgtcaatcag catcggctta cgaaccgtcg cagcgatttc cctgacaagg
    36721 ttgaccgttt cgtcctgcag agaaagtccc ggaccgatga ccaccatatc cgcctttgcc
    36781 gaatgctcca gcaacgcctt cttgtttttc aaagccatgc tccctgcctc ggtttcttcc
    36841 tgcggcagat aaacaatttc ccggccgcgc ctggcaatta ccggaatcac cgcacgcggc
    36901 gctgccagtc ttgcataacc gccgccggcc ttcaaaaagg acatggccga cagatacgga
    36961 gctccgtaat aattgaccgc tcctgcgatg aacaggacat cgcccatggc gcccttatag
    37021 gcatcggcag gcctcggcgg caaagccata gcgtcattcg tcctcaccgg gatgtcatcc
    37081 cggtcataca gcgacggcgg aaaagaaatg tgactgaccc ataattcgcc gttgacgtca
    37141 caaccgggat aaagcaggtt gccgattttg ggaaggccga acgtgacggt atagtcggct
    37201 gtaacagcgg ctcccatgat ttctccggta ttgccgttga ccccggaggg aatgtcgagc
    37261 gataaaactt tttttccgcc tttattaatc agtgaaatgg tgtccgcaac caatccggaa
    37321 accggacgat ccaatcccgt ccccaggatg gcatcaatca caacgtcgca atgggccacg
    37381 tgcctgccag ccggtcgggc atttgtcagc tgcgctgttt ccagcggcaa ccgtttgacg
    37441 atttcgaaat tcgtcgcggc cgcacctctg aatttttcgc tgtcattgag taaaaatacc
    37501 ctgacaaggc cgccgcgtga atggagcaga cgtgcaacga caagaccgtc ccctccgtta
    37561 ttaccggatc cgcagaaaat ggcgaatttc ttgccgcgaa tgcctgtgcg cgacaaaaga
    37621 acgctgacgg ctgcccgtcc cgcgttttcc atcagtacgt cctcgggaat gcccagatgc
    37681 tcaatagcaa agcgatccat ggaacgcatt tcaccgacac tggctatttt cataatgagt
    37741 tctccgcaaa cctcgatccc atttcctgcc aattatccgg cataaaaaag agccacaaac
    37801 tttaaatccg ttgcggtttc cggttatggt agcgttaata aaaatttaca aattaatata
    37861 ttaataatac gcagattgcc gttgaaaaat caactgatta ttaagacctt ggctccgcgg
    37921 attttacctt ctttcaattc gccgagcgct tgattgacgt cgttgagcgg ataggcctgc
    37981 acgtccggaa caatgcgcat gtcggaggcc agtttaagaa agttctcaat atcacaaagg
    38041 gccacgttgg ccacgctttt gatttctttt tccatccaga gatgacgtcc gtaattcagt
    38101 tgcagaagat aatctttgtc tgtgtcctct ttgcggattg cgttaatcac cagccggccg
    38161 gccggttcca gattttttaa cgcttcgacc accggtttcc agaccggcgt cgtatcaatg
    38221 acggcatgaa gcttttccgg agattcgtcg gatgtatctc cggcccagac ggcacccagg
    38281 tcaagggcaa attgacgttc cgccggattg cgggcaaaca cgaacaatgg tgtttgcggg
    38341 taaagatacc gggccatctt caaaaccaga tgggccgacg cgccgaatcc ggtcagaccg
    38401 agacgttgtc cgtttgtcag atttgccaga cgcagggaac gaaaaccgat ggcgccggca
    38461 cacagaaggg gcgcggcctg actatcggaa aaaatttcgg gaatgggaaa cgcgaatttt
    38521 tcggatacgg tcagatactg ggcatagccg ccggagacat ccctgcccgt ggccttaaaa
    38581 ccggcgcata gattttcatt tccggaaaga cagaaaggac attgaccgca ggatcgatta
    38641 atccagccaa tgcccactct gtcgccgatt gcaaatttgc gcgcgcgcct gccctgtccg
    38701 gcaacaacgc caaccacctg atgtcccaaa ataacaggga atgaaggcag aaagattctg
    38761 ccttcaattt cgtcgagttc cgtgtgacaa acgccgcata ccgacacttt aaccagcacg
    38821 tcgtcaccgt cgcccaccgc aggttcggca acctcggtta ttttcagagg cttcttattt
    38881 ttctttaagc tggttgtttg ctctaaaatc gccgctttca tttcaggctg acaggaacat
    38941 ccggcgcgac gtccacgggt tgaacatatt tttcaaccac atcctgcgta ttgttcattt
    39001 tcagtttgtc aaatagaaag gcaaactgtt tttccagtcc ggcgctgatt ttatcgcggg
    39061 tgtccgccgg caaatgccac atatcctgct taaagggacc gaaacccttt ttgcgggttt
    39121 tgtaaataaa ttgttcttcc gtatctttat ccttccattc agactggaat gccgttttca
    39181 gatgatcgta tattctggcc ttcaagtcag ccgggaaagc cgaactgtcg taaataatat
    39241 tttgaaagcc ggtggccaga tgaatttcag ccgtccccac cgcggggaac cggtcaaacg
    39301 cctcgtcggg taatgtcgac gctccgtgct gaaccgcacc ggccagaccg tatttggacc
    39361 gggccaccgc cgataatttc tccagaaccg caaaatcgat tttgactttg gcgacgctgc
    39421 cgtctgccag cggaacgccg ccgtgcgtcg ttcccgtctg gacgctgatt ttactgatcc
    39481 ctttgagcga ttcaccctgt tttttaagct ccgccagata accgtccata taggcctcca
    39541 gctcttccac cgtgctgttt ttcccgccca cttcaccgat ttcgccgccg actgatattg
    39601 tgattccctc cggttccaga tcgcggatca tggtggtcag ttcggctgcg agactgaaat
    39661 tcgtctgttg ctgttctgtg acggtgggtt tggaaagatc caccagcgtc gacgtatcga
    39721 tatcgatatt gtaaaatccc gcttcaatcg cttcccagat taaatttttt accgtctggg
    39781 tttctttcgc tgcgtcggcc gcaaatttct tggcgttgat ttgaaaatgg tccccctgca
    39841 aaaagacggg cccgcgatat ccggttttga ttgcggcagc aatgacggcc gctgtgtatt
    39901 ccaccggccg ttgcgaggta taatcaattt ccgaccgggc gatttcgaaa atgacaggac
    39961 cgactttccc tttcatcatc gcgcggaaaa ccgcctgggc cgattcatag gtcaggccgc
    40021 ggagattgat tgccggaacg gtaaatcccc tgacgtcttc ccggcccatc gcttcataaa
    40081 ggggttgaat ggaggcggac acaattccca gggcagcgcc tgcgcggcgg atcagccagc
    40141 atgcggcttg ctgtgctgcc tgatcggcgc tgaacacggc ggtataaatc aaatgatcaa
    40201 tcaagtctcc tctgagtgcc gtctcattca aaatgctcac atcgttgccc tcgatggcaa
    40261 taattttttc gtatttcttc tgcaattcat ttttatcttt gtaaatcata atcactcctt
    40321 gcaattatat tttattcagt cgccggcggg gaaatcaccc gcccgttccg accatctccg
    40381 caccgggatt atttttcacg atgccgacag catttctttt gccagttgga cttcgtaagt
    40441 gcttccgata tagatcggcg agagctgatt gatcgtctcc accttcacag aaagaatatc
    40501 ctgatcgccg tcgatggcga ggcctccggc ctgttccatg atgaaggcca tcggttgcaa
    40561 ttcaaacagc agccgcagct tgcctttcgg cgattttttt aaagccggat acgtgaataa
    40621 tcctcccttt ttaatcagaa cctgattgat gtcaggaacg aaaccgccgc tgtagcggag
    40681 tttgtagccg tcgttttcca gtttgtcaat aaactgaaga tgcgccggat tccagtcccg
    40741 gcgcaggccg cccggtgaaa atatcgaccc cttttctttc aatttgatat tttcctgcga
    40801 cagaacatac tctccctcgc gattaaggac gaattcatgg gcgcccttgc ctgccgaata
    40861 aatcatcgta atcagaggac cgtaagtaat atacagggca gccaccatgg tatttcgtcc
    40921 gcaatccaga accgattcct tgtgaatgcc gataatggtt cctatcgcca gattggtatc
    40981 caccagggat gagccgtcga gcggatcagc cgttacgatg tatttttcct gaccggtgcc
    41041 tatttttacg atattctcca gctcttcaga tgcgtattca cggacaaaac ccgaaaattc
    41101 cagctggttt ttcagtatct cgtcggaagc gcgatccagc gccatctgtt cctcgccgta
    41161 gacattcttc agtccggcaa gctttctgtt ggactcatga atctttgctg agatatattt
    41221 gccgacgacg gcaatctgcc agatgagacg acgaagttca acctccacac cggccatcca
    41281 catatgtctt cttaaatcaa ctatgttttt tgtgtaatcg ccaattcctt ctcccataat
    41341 cacctccaca ttagttaaaa agcctgactg tgcgggcacg ttttgccagt atgaattgct
    41401 ggctttattg attaacattc tttgagttta cttgcaaata tttaaggtat aatcagaaaa
    41461 gaatttctac ccttcacagc cataatgatt tgatatcttt gtagattatc agaataaagg
    41521 tattattatc atctcccctt ctgttttcaa aaattaaggg ccgggaaaac tattttggag
    41581 aacgcaacgg cttaaatatt gactttgacc tttccttgcg ttatgagtgc ggcctcttta
    41641 ttttttcatg atcgaacatg atccgggagg tttctgtgac gtcatcaaaa tcttataaaa
    41701 ttgcggtggt tcccggcgac ggaaccggtc cggaagttgt tgccgaaggc ctgaaagtgc
    41761 tcaaagccgt ggcggcatcc gaatccatac gatacgaact cgttcattat gatctgggcg
    41821 gcgaacgtta taaaaaaacg ggggagatcc tgcctgattc ggttctcgaa gaattcagga
    41881 aatttgatgc catttatctg ggtgcgatcg gacatccgga tgtcaaaccg ggaatcctgg
    41941 aaaaaggcat tttgctgcga atgcgtttcg agctcgacca gtacattaat ctccggcctg
    42001 tcaaacttta nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgcgaatgcg
    42121 tttcgagctc gaccagtaca ttaatctccg gcctgtcaaa ctttacccca acgtgccctg
    42181 cccgctcaag gacaaaggac cgaaagatat cgattttgtt gtcgtccggg aaaacacgga
    42241 aggcctctat acaggcggcg ggggatttct taaaaaaggc accgttgatg aagtggccat
    42301 ccaggaatcg atcaataccc ggaaaggcgt tgaacgctgt ttgcgctacg cctttcagta
    42361 taccgccaaa agaaacaagg cgaacaaatt aaccctgtgc ggcaagacga acgttctgac
    42421 gtatgccttt gatctctggg aaagaacctt caatgagctg gcaaaagact atccccaaat
    42481 aaaaaccgat tacgtcaatg tggacgcgac ctgcatgtgg atggtaaaaa atcctgaatg
    42541 gtttgacgtc atagtgaccg ataacatgtt cggagacatc attaccgatc tgggcgccat
    42601 ggttcagggc ggcatgggca tcgcagcagg cggaaacatt aatcccgaag gcgtctccat
    42661 gtttgaaccc atcggcggtt ctgcgccgaa atataccggg aagaatatca tcaatcctct
    42721 ggcggccatc tgtgccggcg gtatgctgct ggaaacgctg ggtgaagaaa aggcagccaa
    42781 aagagtggaa gccgcggtcg ttaaacttct ggaaagcggg aaactcaaaa gcctcgatgc
    42841 cggcaagatg ggcatgtcca ccagcgaaat cggcgactgg ctgtccgata acatctgagc
    42901 cggttaatta tctttccgcc atccgttttg atcaggaagc cgtttggtgt gtctttcggc
    42961 gatctttacc gttttgaaga caacggataa gatcatcttt ccccggccgg aaattttccc
    43021 gaagatctgt aaaaattctt tcgcacacga tttcccttca tcaacagcga aaaagttgca
    43081 tctttatctt tgttcaagca tctgatatcg ctaatcgggt tttccactgt tgagcatctt
    43141 ttaagaatgc tgcttgatgt ttttaacggc aaaaaacccg gttgaaacaa attattttca
    43201 gttcttgcat ttttaattta accttgttat caaaatccat taaatattga aggcattccg
    43261 gatcaaatcg aagatgaatt ggtttttaca tagcggatcc tgtttatgaa caattttttt
    43321 atttttattt tcgtttttgt tctttccatc gccgtcatca tcagttttat ttataccatg
    43381 tggcgtatga accgggacga gcagaaactg atcagcatta tccacggctc ccctatcccc
    43441 acgttcgtca tatccaaaaa tcacaaagtc gtttactgga acagagcgct gcaggcggca
    43501 agcaaaatca agcccagcga cgttctgggc acagaccagc actggcgcgc gttttacaca
    43561 tcgccccgtc cctgtctggc ggatctcatt ctggacggga aaaccgccca gattgccgaa
    43621 ctgtatccag accgctggag cagatccaag ctgctggaag acgcctacga ggtgacacag
    43681 ttttatcctg acatgggcga aaaggggaaa tggtatcgca taacatcggc aggcctcaaa
    43741 gattccagag gaaatctgtt cggcgtcatg gaaacactgg aggacatcac agaacagaaa
    43801 ttagcgcagg acgaactgct ccggcggacg aaactggaat ccctgggaac ctttgcggac
    43861 ggggttgcca aagactttga cagtctgctg accgcgattt tacggaatgt tttcctggcc
    43921 aaaatatccg ccagtgatga agataaaatg ctggagaacg gtcttgccgt agccgaacgt
    43981 gccggccttc aggccaagga actggcccac cgtcttatca ccttcgctca gggaggatac
    44041 ccggtgcgca agctggaaaa aatagaacct gtgttgcatg aggcggcgga aagtgttttt
    44101 aaaaactcgg ccatcacctg caatattacc atcgcggaag atctctggcc ctgcgaaatt
    44161 gacgccacac agatcaaaca ggttattgaa aacgtcttga tcaacgccag agaagcgatg
    44221 ccggatggcg gttccgtgca gatgaccacg gaaaatacca ccaccggaac caacatccgg
    44281 tcgctgggac aggggaatta catccgaatt attatcaagg acagcggcat cggcattccc
    44341 cgggaaaatc ttccccggat cttcgatccc tactttacga caaaaaagac aaaagaacac
    44401 gggggaatcg gcctggggct ggcaatcagc gattccatca ttaaatacca taacggatta
    44461 attaccgttg aatcgatcgt caattccgga acaacctttt ccatttatct gcccgcaaag
    44521 acaacaaacg gttcttagcg tttattttcg tctcatcaat tctcaagagc gtgacccgct
    44581 gaacgggagg cattaccggt tcatctcata tttgattaat ttcgcgcgca gcgtttttct
    44641 ggtaatgccc agccggcgcg ccgtttcgct tttattgcct ctgcaatcag ccagcaccgc
    44701 cagaatattt tttttctcca tgctttccag agataaattt cttatggctt ccccctgctc
    44761 cgcaataccg gaaacctcat ggcttttctc catgaccagc gtcatatcgt cggcgtccag
    44821 ataatccgtt cgtgacagaa caaccgaccg ttccacagcg ttcatcagtt cccgtatgtt
    44881 acccggccag tgataggcta aaagtttttc catcgcccgc ggagtgaatg ctttgatggt
    44941 ttttttattg cgcccggcaa acaactcgag aaaatgctgc gccagtatcg gaatgtcttc
    45001 tttccgttct cgcagcggcg gcacattcaa ggaaacaacg tttaagcgat aaaacaaatc
    45061 ctcgcggaaa tttccgttct gaacttcttc tttcaagttc ttattggaag cggcaataac
    45121 ccgcacgtcg actttaatca cttccgctcc gcccacgcgt gtcaactcgc gttcctgcaa
    45181 gacacgcaga agtttgacct gcatcgcctt ggacatctcg ctgacttcat ccagaaataa
    45241 gctgccgccg tgagcctgaa ggaatttccc ttccctcctg cgttccgcac cggtaaaggc
    45301 tcccttctcg tgcccgaaaa gctccgactc cagaagcgtt tccgtcagcg ccgaacagtt
    45361 tattttgata aaaaccgttt ccgcccgcat gctgttgaaa tgaatggcgt tggcaatcaa
    45421 ttccttgccg gtgcccgact ctccggaaat cagcacattg gcctccgtgg gcgccacctg
    45481 ggccactgtt tccaagagct tgaccatcgg gtaactctgt ccgatgagat tctgccggtt
    45541 aaatttttca tccagcctgt ttcttaaata tctgttttcc cttttcagcc gattatgttc
    45601 cgtcgcccgt ccgatggcaa ttctcagttc gtcgaagtta agcggtttgg tcagataatc
    45661 ataagctccc tttttcaggg cactgaccgc cgtttccaat gaagcgtacg ccgtcataat
    45721 aatcaccggg gtttccggat tgatggcttt aatctgtccc aaggcttcta ttccggaaac
    45781 attcaacata caaatgtcca tgatgatcag atcaaaaggc attcgccgga ccgcttcgac
    45841 cgcgacgtga ccgtcgtccg cttcattgac ttgatacccc cagtcgttca aatgtttttt
    45901 gagtatcatc cggtgaacgg aatcatcgtc aacaatcagc acagcttttt caacaaccat
    45961 gattattacc cctggttctt tgtttcatca tcctttataa aaaaatgaga cggacctcat
    46021 ttctttttta aaaaatgaaa ccatctggat aattattatc cagatgaatg ctaaattgga
    46081 tacgtaaatt ggatcattat tatccataat attttataaa acacaatcta ttttttaaaa
    46141 catttcaata atgcttgtaa tttcaatata ttaaaaaatc agcaagctat ggcacatcaa
    46201 ttgcacataa aaattatacg caaaccatga aaccctttaa gtcttctcct aggtttcaac
    46261 tctcagcaaa agcgggaggc tattaagcct cccgcttttt cttgaataaa ccttttcaga
    46321 gtattttgtt aataaacaag ctcttaaaaa aatcaaagag tgccaggagc gccaaggaat
    46381 ataaagaacg atggaggatt gaaagctttc ctcatgaatc gtaaatgatt taatcagaaa
    46441 ttttattctt cagttcgttc aatttattga gcgcttcgag aggcgacagg gaggaaatat
    46501 cgatattctt cagtgcctcg atgaggttct gtcctttctt ttcgagagca atggcggttt
    46561 cccgtcttga aaaaagactc aattgcggat tggcgttttt gccttcgcgg gctccccgga
    46621 ctatgcgggg catgccgatc tcgtcgaatt cccctttttc cagattccgc aatatttcct
    46681 ttgcgcgcat aatgacgtcg gccggcacgc ccgcaatgcg cgccacttcg atcccgtaac
    46741 tgcggctggt cccgccttcc ataattttgc gcagaaaaat tattttttct ccccattcct
    46801 tcacggcgat attataattt ttcgccccgg gttttgttac caccagatcg gtcagttgat
    46861 gataatgcgt ggcaaagaga gcgcggacac cgcgtccctg gaaatcatga atgtattcgg
    46921 caaccgccca ggcaatgctc aatccgtcga aggtggaggt tccgcgaccg acttcatcga
    46981 tgatgaccag cgaccggggt gtagcgtttt tcaggatttc cgccgtttcc gtcatttcca
    47041 ccatgaacgt gctctgtccc ttgatcaggc tgtcggaggc gccgatacgc gtaaatattt
    47101 tatcaacaat tccgattttt gctttggccg ccgggacgaa acttcccatc tgggcaagca
    47161 gaacaatcaa ggccacctgc ctgatatacg tggatttacc cgccatattc ggcccggtga
    47221 tgacgagcag tttattctgg cgcaaatcca ttaaacagtc gttcggcaca aacccttcgt
    47281 ttttcaaagc cgactccaca acggggtggc gtccgtcctg aatgtcgatg gtctcctcat
    47341 catcgatttc cgggcagacg taatgatatt tttcggccac gtccgccagc gcagccaggg
    47401 cgtcaagatc ggcaatcaga tttgcattgc cttgaatctc cgcgatatac cggtcaagtc
    47461 cgtcacgcag attgatgaaa agttcctgct ccttctgctt gattctttcc tccgcgttga
    47521 gaaccgtctg ttcgaattct ttcaatgcct gattgatata ccgttccgcg ttgaccaggg
    47581 tctgtttgcg gatataagag tcgggaacca gagctgcgtt ggctttggtg acttcaatgt
    47641 aataaccgaa cacattgtta aagcccactt tcagggaatt gatgcctgtt ttcttgcgtt
    47701 cttccgcctc cagagcagca atccattgct tgccgtcgcg gctgatcaat cgcaacttat
    47761 caagttcttc atcataccct gcagcgataa atccgccgtc ctgaattttc tgcggcggac
    47821 tgtcgacaat cgcccgtgaa atcacatcca caacatcggt catctccgtg atgtgcgacc
    47881 ggatggcgca aatggccggc gccgtgcaat cgctgagcag ggacttgatc tccggaacgg
    47941 caagaagcga atttttcagg gcgaccagat cgcgggcatt ggccgctttg agcgacactc
    48001 ttccggccag cctctccaga tcatatatac gcgacaatgt ttttcgcaga ttcgcccgcc
    48061 gaagaggatc atctttcagc tccgccactg cggccagccg ggttttaatt ttttctgcat
    48121 ccaccagcgg atagctcatc caccagcgca aacggcgtgt ccccatcggc gtttgcgttt
    48181 cactgaaaag agaaaaaagc gacccggccg ccgtattgtt ctggatcgac gtgaagattt
    48241 ccagattgcg gcgggcggta tcatccaaca gaagaaactt ttccgcggag taccatctga
    48301 tcgcgctgat gtgctgcgga ttgatctttt gcgtctcgtt gacgtagctc aatatggcac
    48361 cggacgcctt caatgccgcc ggatgttcgc tgatgccgca cgcatccaga acgtccgttg
    48421 aaaacgtccg ctgtagaaca tccccggcct ggccggtcag aaaatagttt tcgtccagat
    48481 cgttgatgcg caattgaggc atctgtgctt cgatggtgtc gagtaaaatt ctgtcgggga
    48541 aatttcgcgg caggagcaat tccttaaaat ccaggccgga aattaccgtg aagaaataat
    48601 cgcggtccgt gaattcaccg atctggaatt cgccggttga aatatccaga aaagccagac
    48661 ccagggtgtt tttttcggcg cagaggcagg ccagataatt attttcaccg ggagccagat
    48721 tttcttcatc cacgaccagt ccgggagtaa tgatccggat aacctcccgt ttgacaatcc
    48781 ctttggcctt tttgggatcc tcgacctgct cgcatatcgc cactttgaat cccttttcca
    48841 caagcttggt gatgtaggcg gacgcagcat gatacgggaa accgcaaagc ggcacgctgt
    48901 cctctttgcc cttattgcgc gacgtgagcg tgatctccag aacgggcgcg gccgtcaccg
    48961 cgtcctcgaa gaacatctcg taaaaatcgc ccatccggta gagcagaatg caatcaggat
    49021 gctgttgctt gattttgaca tactgtttca tcgccggagt caaatcgagc gtcgcgccta
    49081 ccgccattta ccgtcgtccc ctttccgcag atcaatgtag tccggatcgt cctttttgac
    49141 gattttatgc gaggtgataa atgaattggc cagccagctg acgatgctga taatcagcga
    49201 gccggcaaat gcggaaaaaa agtttttaat cgcaaatccc gatacaaaat aagcaaccag
    49261 ttccagcaaa aaagcgttga taaaaaaata aaacaccccc agcgtcagaa tactcaacgg
    49321 cagggtcagg atcaccacaa tcggtctgat aaaaatgttg atcagcccca ggacgcaggc
    49381 ggcaatgaca gcggtggaaa gtttatcgac ttcgatcccc ggcacgaatt gcgaagccag
    49441 cagaatcgcc gccgtgatga tcagccatct tgtaataatc ataatcatat caggacccat
    49501 caatatctga aatcaggcaa cgcgttcaac cctttttcac ggagggctat ttttccgcgt
    49561 ttaccatctt ttccgattta aaaaatatcc aggaaacgtt gaaaccgctc atcatggacg
    49621 tttccctttt caccagcgga ctgtcttcaa aagaagcgcc ctgaagaaaa tcggcgctga
    49681 caaaagcatg aaagataaag tgtttatagg ttttgctcaa accgattgtc accgttgaac
    49741 cgctgtaccc gccgtcagcg cgatacacgg gacgagtcac cctggcatca cggggttcca
    49801 cttcataaaa gtattcgtga taaccgctgt cggcaaacat cggtccggca gaaacgccca
    49861 gatttaaacc ggtacgggga attaaatcat ccgtcacaat gttgatccgc gggctgaaga
    49921 cccatccctc gtgacgcacg gaagaaaaat ccgtcgaaaa aaaagcacgg gcgggcagcg
    49981 tcagattcat tcgggttttg tgttgcttgt gttcccacag cttaatcttc aaagccggtc
    50041 ccaattcaaa cgtcggatcc agatcatcca tcccctcgcg ggcggcattg tcgtcactgt
    50101 tgaccggtac ggagccgaaa atactgaaat ccagcaagac cctgtccgtt ttaaaaattt
    50161 gtccggtaat ccggttctca tcgactttaa aaaaatcgcc gcgatagaca acatgaggat
    50221 acggcagcgg ataaaatctg ctttcatccg ccccccgata atcgggcatc tggagaaaag
    50281 ccattcccgc accaatctcc catagcggct tttcttcgcc aatggctttc ccgggtgaaa
    50341 aataaatgat caataaaaaa accgtgacgg tatatcgaat gtgcacgtat ttgccctatt
    50401 catcaggttg cgcccgtcaa ataccccggg cggttgctca atatcgcaaa cccgcttcaa
    50461 aaaacctcat ccctgaaacg ccacgaacat tttcgccttc gcgccgcaga ccggacattc
    50521 ctccggtaaa acgccgtcgg cgacatatcc gcaaatttta cagacaaaat aatcggtttc
    50581 gcgttcttcc ataaaatggt tcatggcctg cttgtaaagt ttggcgtgcg tttcttccac
    50641 atccttgctc tggctgaaat gcaggacggc ctccttgttg ccttcggctt ccgccagttt
    50701 cacaaaccgg tcgtaggcaa cttcggcaac ctgcgtttcc gattcaaaac tggccgcaag
    50761 attttcttcc gtgcttttta tttccttaag acctttcaga gcccgggcgc cgtgaatctc
    50821 ttccgaaaaa gcaataaccc gaaaaagctt cgccatttgt ttgtagcctt catcctcagc
    50881 cttctgcgcg taaaccttaa gccggagagc cgccttcgcc tctccggtat atgcctgact
    50941 caacgcttcc tttatatcca tacgttttac caacctttta ttttttgcca cttcaaccgg
    51001 caagttatag tccagccatg ccggggaaaa gtcttgtttc aagattgcaa agccttctga
    51061 gaaaaggctc gcaatatcat tttaccggga ccactattcc ggcgagaatt cgtctttggc
    51121 cgctccgcac accggacaga cccagttttc cggcaatttt tcaaaaggag taccggcagg
    51181 aatattgcct tctgaatccc cttcagcagg atcgtatatg taaccgcata caccgcaaac
    51241 gtactttttc atcttcacat cctcctttat atatttcgat attttactgg ctattcacct
    51301 tctcgagcag ttttgccgcc accgttttgc ccagtttttg acattgatcc aattcgtcgg
    51361 cgccgggaac atgtttcacc gcaacggact cggacgcaat atcaaccttc atctctttga
    51421 gcatggcctc cagatgtttc acagattctc cgctccagcc gaaagatccg aacgccgcgc
    51481 cgatcttatt gaccggtttc aaacccttca gataagtcat cacatccgcc atctggggca
    51541 gaatgttgtt gttgagtgtc ggcgagccga caatgagcgc accggcatca agcacttcat
    51601 aaaccgcttc actgcggtgc gtttcgttca tggaaataag gctgaccttg acaccggcct
    51661 gagccagcgc ttcgctgatg atcctcgcca ttttttcggt agagtgccac atggtcgcat
    51721 aaatcaccac ggccttggct gtcggtttct gcaaagccca tttcttatat aaatcgataa
    51781 tccaaccgat atccttacgc cagatcggtc cgtgatcggg agcgatcacg ctgattttca
    51841 atcccgatgc cgcgaccttc tccagcgctt tcaacacaat aggagaatac ggcagaacaa
    51901 tattggcgta gtatgtcgct ccttcatatt cgagggtcga aacgggaatt tcatcggcaa
    51961 aacgctccag cgtcgccaga tgcatgccga aagcgtcctg cgaaaacagc agttcatcga
    52021 catccagata cgtaaacata ctgtccggcc agtgaatcat gcgcgtttcc ataaaggtca
    52081 gattcatatt gcccagattc agagactgtc cgttttcgac agcaataatt tcccgctcgt
    52141 catgaaaaag atcttttaat gtttttgtcc ccaccccgga cgcaaaaacc ttttggggtt
    52201 ttatgaaatc aatcacgtcc ggcagagaac cggtatgatc cagttcagaa tgattggaca
    52261 caatatattt tattttggat ggatcgacga cggatttgat ccgggcaagc atttcatcca
    52321 taaaaggctt tttaacggtg tcgatcaggg taatctcatc cgccatcacc agataagcat
    52381 tgtatgtgct gccgcgcggc gtggtgtagc cgtgaaaatc ccggatggtc caatcgatcg
    52441 ccccaaccca gtaaacatga tctgtcactt taacagccga ataaatatcg ttcatggtcc
    52501 cacctcttga tgaatttccg ttgagaataa tataaggtct catccggtca tgtcaatcaa
    52561 tacttatata aaatcgacgg tattttttat tcgatgcatt ggcagaaatt gcaaaaggca
    52621 acaaagcctg aataatatag atttttttcg cgccgccatt atttttttca tcattccggc
    52681 cagcgcaagc caaccggcat gatcgcgaat cgtattgaca tgcctctcgt ttattcctat
    52741 agtttctaaa ggaaaatttc gtcatctttc aaccggaaaa aagctgcttg aacttttatt
    52801 ttgccggctg atattttcct gaataccgga gacacgtgat accattttgc gcaaagacat
    52861 cagaaatccg gacatccatt catcgccctg gctgcttgat cttgcgtggt tttatattca
    52921 acgaaaaatg ctgaagcgaa aactgccttt gctggccagc ttcaagatca cctatcgctg
    52981 caacctgagc tgccgggcat gtccttttca tctgcgttcc gatcgcgaaa aagaacatat
    53041 gtcgcgggat gccgccgtgc acataatgga tctgctcaat cagctcggca cgcggatgat
    53101 tgttttcgaa ggcggagagc cgctgctgtg gcgggacggt tcttatcgtc ttcacgatct
    53161 tattctttac gccagaaaaa aattcctccg tgtagcggtg accaccaacg gcaccatgcc
    53221 tctggatgtg ccgtcccata ccctctgggt aagtttggac ggtcaaaggg agacgcataa
    53281 tacattgaga agcaattcct tcgatcaagt ccgcgccaat attcaaaaca cccgtcaccc
    53341 gcgtgttttt attcattaca ccctgaaccg ccgcaactgg caggatctgc cgttcgtagc
    53401 acaatgggtt caggacacac ccgcattaaa agggatgacc gttcagttct tttaccctta
    53461 ccatcaggga gaagacgatc tctcgctgcc accggaagaa cgccgcgccg ccatcgaaaa
    53521 actgctgcaa ctgaagaaat ccgggttgcc catattaaac tccgcggggc gactccgagc
    53581 aatgatcgac aaccgctggt cctgtcatga tgatattctg atcaacgtcg atccggacgg
    53641 caccatcacg aaaggctgct acgtcaaaaa ccgcggcaac attaactgcg atgcctgcgg
    53701 attcacgccc gttgctgagg cgtccggggc gctggatttc attccgggtt cgctttatgc
    53761 gggatggcgc ctgtttctga agatgtgaga cagccgtcat tcatccctct gaccggcttt
    53821 ttaagaaaag attttttaca gaacgaagac ccaccggaac caatcaccgg cataagtttt
    53881 tgcttcctgc tgcctccagt atttcgcaat tgcccagttg gcaggccttg cgggcatcat
    53941 cacatcctga tgcagcataa ccatacatca ggtaaattcc gcctcggtaa tggtaggcat
    54001 tcgtataatc cgctttcagt cgaatggctt cattaaaatc cgagaaagcc tcctgatgac
    54061 ggcccagttc gatgtaagca acgcctctgt tataataaat attggcatca tcagggacca
    54121 gtgtcagtgc tttttcaaaa tcttcaaaag cattctgata aagtttgagg ctcgcacagg
    54181 caatccctct gttcttatac agctccgcgt tattcgggtt catcaaaacg gcctgattga
    54241 aatcctgcag cgcctgccgg tgcatacctg tttcagcgta cagtaatcct ctgttgttgt
    54301 aagccctgta atcacgtcgg tccagacgga tggcttcatt aaaatctctg atggcgtctt
    54361 tcttccgtcc caggcggctg taagccgtcc ccctgaaatt ataggtccgc acatgatccg
    54421 gcgttatggg acgcatggca atcgccctgg tatagtgatg cacagcctcc tgatattttt
    54481 tctgctttgt atagacgttg cccagactga catcaataag ctggcggagt ttattttccg
    54541 caatacccat tttattatac gccatgacgt tcccgcctgt ctcaggaagc attccccagg
    54601 ctttgttgta atagacaacc gcctcttcaa gcctgccctc accggccagc gcattgccgt
    54661 aattgatatg cgccagggcg ttttcctcat tgatccgcga ggcgtttctg aacagtgctt
    54721 cactgttttt ccagaaaccg cactggtgcc aggtcatccc ggacagaaca gcaaaaaaaa
    54781 tcaaagcggc gggcggcaaa aaaaactttt tgatcttttc attttcaacc agcagaggaa
    54841 tcccgaaaaa aatcatgact gcaatgccga tggacggcag atagagataa cggtccgcca
    54901 tcgcattgtt tcccgcggga ataatgccga tgactggaag tatggtgatg ataaaccaga
    54961 gccacccgac caaaagacac ggtgattttt tcatcatcca aacgacggcc gcgctgacgg
    55021 cgaaaaacag taaaaccgcc gcaatgattt gtccggcggg agcccggccg aaaaacggat
    55081 agcaaatggc cagatcatac ggccagaaca atttctgcag ataaagaacg aatgaaataa
    55141 tcgcgttgat gattcttgat tccagcgaaa acggccaacc gtcgatagga aatttcggct
    55201 gggcgtaaat ggcgataagg gagaaaaccg ccgaaagaag gaagaaaggc agtttctctt
    55261 tcaactgcca taaaaacaga ttttgtctgc gtgatgcaaa acgtttcagc ggccagtaat
    55321 ccagaagcat catgatgacc ggcagggtca ccgtcagcgg tttgctcatc agtgagcagg
    55381 caaaaaagaa gacggccgcc agatatcttc tgaataccgg tttttccgtg tacagagcat
    55441 aaaaatataa cgtcagcatc cagaaaaaaa cgcttaatac atctttacgt tcggctaccc
    55501 aggcaacgga ttccacccgc agcggatgca aagagaaaaa agcggcgaca aaggcgctcg
    55561 gccagaaagc gccggtcatc cgctgaaaca tcccaaacag cagcaaggag ctgatgatat
    55621 gaagaaaaag attcgtgaga tgatagccgc ctgcatgtaa gccgtataat tcataatcga
    55681 gcatcagcga aagccacgtc aggggatgcc agaattcggc gtacaaggtg gtaaaggccc
    55741 agcggattcc atcaagcgtc aggccggacc ggatgtaatc gttttcagtc acgtagacac
    55801 tgtcatcaat attgacgaaa tcaaattgat gcacctgcag gtaagcagca agaatcaacg
    55861 ccgtcaaaac catacagaaa ataatattac gtccggatgg accgatgttt atcttatctg
    55921 acatggggac ctgtttggat ttcttctatc atatgcgcat atatagttca cccgtttttt
    55981 gatcacagcc actgaaggca tacaatatta ttcgggaaat taacactatt gaaagcagcc
    56041 ccttttaaat attattgtcc gggatttctg gaattttcat aaacgatctg ttgacttgac
    56101 cggtaaaaag cattattttg cgccggataa ataaaacggt tttacacgaa ataaagcaga
    56161 taaatgtttg gtttggttca ttctgaaaat cggtcatccg actccgacca tagatttttt
    56221 tttgcagcta tttcggaaag cggggaaaaa tgaaagctga agatattaaa aaaatactca
    56281 ttgccggagc gggaaccatg ggccggagca tcggtctttc ctgcgtcaca agcggctatg
    56341 ccatcgtcct gtacgacatc aacgatgaca tcccggcaaa ggccagggac atcatgaaca
    56401 aaaagattga taaaatggcg gacttcggga tgatgccgaa ggacaaggcc gaatcgttta
    56461 agaacaacat cacggcgaca accgatcttg ctgccgccgg ggccgatgcc gatgtggtca
    56521 gcgaatcgat tcctgaagat cccgacatca agggagattt ttttgcagaa ctcaacaagg
    56581 tctgcccggc gcggaccatc tttaccacaa acgcctcttc tctggtgccg tcgatgtttg
    56641 ccgataaaac cggccggccc gaaaaatttc tcgcctggca ttttcatccc ggttttaagc
    56701 tggtggatgt gatgggacat ccgggcacat cgccggaaac ggttgaaacc gtccagcgtt
    56761 tcagcgaatt aatcggacat tcgccgattg tccttaagca ggaaaaagcc gggtacgtgt
    56821 tcaactcgct tctcaatccc tggtttttgg cggcgttgaa tctggcttcg cgggacatcg
    56881 cgtctcccga agacgtggac cgttcctgga aggaaatcac cgcttcaccg ctgggtccct
    56941 ttgagatgat ggattacgtg ggtctggaaa ccgtatggcg aatcaccgat ttctgggcga
    57001 aaaaacgcaa taatcaccag gcaaaattaa gcgccgacct gctgaagaaa tacgtggacc
    57061 ggggcgagct gggaatgaaa acaggcaagg gattttacga ttatacgcaa aagaagtcac
    57121 ccggcgaatc ctgaaaaaaa caaaccctga acggttgttt ttagagcgga aacgctttta
    57181 aaattttgta gacaattcaa ggtaattcag gaagcgttat tggcacccgg cgtcgattca
    57241 aaatatcctt taaacaaata ctcaagatca ttggccatcc gtccgtccat cgagaaattg
    57301 acgctgtcgg cgaaaatatc cagcagcacg acagcattca gactggcgtc ctgcccctgg
    57361 atcattctga gccttttgca aaagtattcg ttaacgattc gcacattctc atacgcttcc
    57421 gccagtttct ttaaatccca gtcgggctga cgtccatgct gatacagaaa atactgagcc
    57481 atcaaataca ttgaaatcgc ccgatatttg gtttcctcta ttgtggcaaa cggcaggtga
    57541 aaacgcacca tgggctttaa tgtttccatc aacgggcatc cgctggtaac catatatatt
    57601 ccgatcaaag aactgacgcc gtgctgcaaa gacaccgtac gaaaatattt tctttcctgt
    57661 gccgtaacaa ttgtctccac gatatcgtaa gaaaaataat tgcggaaata atctacgaca
    57721 tccaccatat tgatggctat gggacaatat tcgcattgat cgtttaacgt acaatgagaa
    57781 cacctgaaac aattcagctt tgtccattcc ggataatttt ttttatccgt cggcagtaaa
    57841 ttcaaagtga cgctgtccaa cgcgatgtta aattcttttt ccacgccgtc tttgaatata
    57901 aacctgtaat tgtatgttac tttgcctgaa gaaccgtggt cagcgtccat ccttgcaccc
    57961 cttctacagc cctacattac gttagagcat ttttaccccg gcatcaaaag ctttcagatt
    58021 gatttgatgt ttaccggctg gaatggtttt ggcgatttcc ttttcaaacg cccggcggtc
    58081 aatcggcaac agtgacgtac ctgccagcgc gccgatcatc acgatattgc tcaaaaccgg
    58141 atttcccaat tccaccgcaa cggacgtggc gtcgatgatc cagttttttg ccgatatttt
    58201 ttcaaacatc gatttgactt cttcaatcgt cggataagta aattcgccgg tgatcacgcc
    58261 catgggataa accatgcgtg aattggtcag caccgctaca tcgggattac catatttggt
    58321 gagtactctg agcgcctcca ccggctccag cgcaatcaca atgtcccccc tgccctgcgg
    58381 aatctgcgga ctcagaaccg acgatgacga cacgcgcaga tggctcatta ccgagccgcc
    58441 gcgctgggac atgccgaagg tttcaccgat ggtgacgata aagcccgcgt tcaccagcat
    58501 tccggccagc acgcgcgagg ccatgacgtt tccctgcccg ccgacaccgg tgataataat
    58561 gtttgttgga tttttcttaa gagact
//
LOCUS       DAOOLB010000006        57149 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_369_length_57149_cov_48.245770, whole genome shotgun sequence.
ACCESSION   DAOOLB010000006 DAOOLB010000000
VERSION     DAOOLB010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 57149)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 57149)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57149
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_369_length_57149_cov_48.245770"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            310..783
                     /gene="rimP"
                     /locus_tag="PLF77_01510"
     CDS             310..783
                     /gene="rimP"
                     /locus_tag="PLF77_01510"
                     /inference="COORDINATES: protein motif:HMM:NF014617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HPC07560.1"
                     /translation="MDFDVKEKIRLLAEPVVASEGMELIHVECIKMHTRWIIRLFLDK
                     EGGITLEDCTNISNQLGDIFDIREVVNGSYTLEVSSPGFDRPISRDQDFLKYRNSRVD
                     VKTGIKIDGVKNFHGVLLDYIEENGRKLILVEVSGKVYRIPRQEILKANLADEKK"
     gene            857..2140
                     /gene="nusA"
                     /locus_tag="PLF77_01515"
     CDS             857..2140
                     /gene="nusA"
                     /locus_tag="PLF77_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPC07561.1"
                     /translation="MLPELKRLIEQMGKDRGISKEIIVEALEAAMLTAASKKFGQNVE
                     IEAHYNDETGEVEVFQFKTVVDKVLHPETQISKEEARATLDEGAEPGDSLGMKIETSQ
                     FGRIAVQLAKQIIIQRVKDAECDNIYDEYKDRRGELVNGFVQRFEGGSIVVNLGRAEG
                     IVPPSEQIHREAYKRGERIRAYIVEVKKSPKGLQIILSRTHPSFLKALFEVEVPEISE
                     GLIDIINVAREPGKRGKIAVKSKDKDIDPVGACVGMRGSRVQSVVQELRGEKIDIIPY
                     SEDPAKYVSSALSPAKVNRIFIDDENRTMTVIVPDDQLSLAIGKNGQNVRLAVKLTGW
                     NIDMKSETMEQAQEEGKSGQNDLTKITGIGPAMAEKLFSKGIKSIAMVAATEPELLSQ
                     IPGIGEKTAQQWIETAGKILNEEIAGSKSDSSSEN"
     gene            2167..4800
                     /gene="infB"
                     /locus_tag="PLF77_01520"
     CDS             2167..4800
                     /gene="infB"
                     /locus_tag="PLF77_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPC07562.1"
                     /translation="MPKKRVYELAKELGMESKVLIARLEKIGISVKSASASLEEADAE
                     RAKKELTAGELREVVEQRIKTTVIRRRTVVTAVEKPPEEVPLEEAKPPEKEPAEDKLK
                     KAPPGKTEKVKKEKPGEEPAVPAETVSEKAVKEVVPPAAVGPAAAETKASLEQKAAPL
                     EKSAEEKAVKPETKEAPVAPVKPTIITKHKIIRPETGKAVPPKVPAAKPAEKISQPSA
                     EKPKKKGKYQVEVLIEEEKKIPTKKILEKKIEKRLKRHEEDQEVVFTKWREGKKAAPT
                     KMKKTEITVPKAIKRRIKIGETITVGELAKRMGIKVSEIINKLMSMGVMATINQAIDY
                     DTASIVAGEFSFQLESADVEFDESVLKAPSTPENLKPRAPVVTIMGHVDHGKTSLLDA
                     IRQTNVSDGETGGITQAIGAYHVHVNGRDIVFLDTPGHEAFTAMRARGAQVTDIVVLI
                     VAADDGVMNQTVEAINHARVAGVPIIVAINKIDKPGADPEKIKQALTEHNLLSEQWGG
                     DTIFCEVSAKKKINIEELLEMILLQADVMELKADPDRPVRGIIIESKLDKGRGPVATV
                     LIQEGTLRESDSFVSKTEFGRVRALINDQGRRIKEAGPSMPVEVIGFSSVPQTGAEFF
                     CVDDEKKARAIADYWTRKAREKELSSLSKITLEQLYQRIKEGAKEFNVIVKADVQGSI
                     EAISDALNKLSTDDIKLKIIHSSTGAISETDVMLASASQAIIIGFNVRPDARVVDVAQ
                     REGVEIKLYDIIYNVIADVRAAMEGLLEPEYREVVQGRAEVRELFKVPKVGTIAGCYV
                     TDGKIMRSNNLKLVRDSVVVFDGKILSLKRFKDDAREVLAGFECGIGIEGFNDIHPGD
                     VIEAYAIETLEKKLEPNADKR"
     gene            4870..5208
                     /locus_tag="PLF77_01525"
     CDS             4870..5208
                     /locus_tag="PLF77_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HPC07563.1"
                     /translation="MVVGYGIINLRIPESGSLKSKRSVLNRILKRAQNEFNVSIAETG
                     HLDNYKLAEISFAVVGNDSRYINGKADHLLRFIDHLQIAEIVNCKMEIMVVSSFLETA
                     DGDIQKYDEF"
     gene            5198..5560
                     /gene="rbfA"
                     /locus_tag="PLF77_01530"
     CDS             5198..5560
                     /gene="rbfA"
                     /locus_tag="PLF77_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPC07564.1"
                     /translation="MNFKRADRVAELIRADMSEIIMKDVKDPRLHSVTITAVKVSDDL
                     RNAKIYFVEMGKDECSVEIKTGLAQASGFIRRELGKKLQLRFVPELMFIHDTSFGYGN
                     RIEKLLAQIHSQENENDS"
     gene            5550..6500
                     /locus_tag="PLF77_01535"
     CDS             5550..6500
                     /locus_tag="PLF77_01535"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPC07565.1"
                     /translation="MIHDIIQAIHEGQNFLITAHVRLDGDALGSELAVYLMLKELGKK
                     AVVYNKDRTPERYQFLPATHNISHTLTNIEQYDTCIVLDCSDLSRVGDEAENIRKIKT
                     LINIDHHVSNNGFCALKMLDAKASSTGELVFRLMQDMRFKMSRDICTNLYAAIITDTG
                     SFRYSSTTKETFMAAGVLVGEGASPQRIAENIYESDSPARLKLMARALSTLSLDLESK
                     VGSMVVTQKDLLETGAAWEHTEGFVDIPRTVKGIEVSVLYTQRGEKNFKLSLRSKAKV
                     DVEKVAKRFGGGGHIHASSCWMDGDIETIKSQIIQAVREL"
     gene            6506..7441
                     /gene="truB"
                     /locus_tag="PLF77_01540"
     CDS             6506..7441
                     /gene="truB"
                     /locus_tag="PLF77_01540"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPC07566.1"
                     /translation="MMNGVIVIDKPAGKTSHDVVLDVRRALGIKKAGHTGTLDPLATG
                     VLPVCVNEATKLAGFLAGDKKEYRATMLLGVTTDTLDIEGNITGQSDKVVSGEEIRSV
                     MMRMIGTINQVPPAYSAVKHCGKPLYKWARKGVFPDLKPREIKIFSLLVENISFPSVK
                     FRVACSKGTYIRSLCADIGEALGTGACLSGLRRLQSGIFSEAMAVNLNDYKFPEAKEK
                     LAEKILPMAELLPLLATIELEDQCAVKLRNGWQPSVEMLKEHDLPLLKAGDMIKFVSR
                     GNLLAVAEMLAPVSELAAFDGKRQAARILRVFNQP"
     gene            7499..7765
                     /gene="rpsO"
                     /locus_tag="PLF77_01545"
     CDS             7499..7765
                     /gene="rpsO"
                     /locus_tag="PLF77_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPC07567.1"
                     /translation="MLSSEKRKEVIDNYRLHEKDTGSPEVQIALLSARIEYLTEHFKA
                     HKKDHHSRRGLLKLVGQRRRLLNYIKENDIERYRTVIKRLNLRK"
     gene            7901..>10014
                     /locus_tag="PLF77_01550"
     CDS             7901..>10014
                     /locus_tag="PLF77_01550"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPC07568.1"
                     /translation="MSNVFSADFAGRNISLKANYVAAQADGAVLVYYGDTVVLVTAVS
                     LKSTREGVDFLPLTVDYQEMTFAAGKIPGGFFKREGRPNEREILTSRVIDRAIRPLFP
                     KGYFSETQLVATLLSVDKENDPDVASLIGASAALGISDIPFKGPIAGVRVGRINGEFV
                     ANMCPEKMKESEMSLFLVGRKVTPGTSGRPYDVELVMMEGEAKEVPEDIIVDAIKFGL
                     ESIRPVIDLQDQIQASIGKVKRPVEEPVPDEELVARVREEALPGLKEGYAMPRKLERY
                     SKLGDVRAEVIKNIGGDDAVLSKKVAGIIENLESRILRDMIIQDKKRIDGRSSVDIRP
                     ISSEAGVLPRAHGSALFSRGETQALAALTLGTSADEQRMDYVGGEEIRTFLLHYNFPP
                     FSVGEAKMLRSPGRREIGHGALARKALVPVLPSPEEFPYTIRIVSEILSSNGSSSMAT
                     VCGGILCLMDGGVPVKDIVAGIAMGLLKEGNEIVILSDILGDEDHAGDMDFKVCGTEK
                     GVTAMQMDIKIDGLTEDILRKALAQAREGRMHIIGKIRETLAAPRGDISQYAPRITTV
                     KVKEDQVRTVIGSGGKNIRQIISETGVTIDVEDDGTVTIASADAEAAARAVAMVKWLT
                     EEAEIGKIYRGTVKKIVDFGAFVEILPGTEGLLHISQIAKERIAKVTDVLQEGDEVMV
                     KVLEIDKSGKIRLSRKEALGAEV"
     assembly_gap    10015..10114
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10301..10744
                     /gene="dut"
                     /locus_tag="PLF77_01555"
     CDS             10301..10744
                     /gene="dut"
                     /locus_tag="PLF77_01555"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPC07569.1"
                     /translation="MKKVKVCIQRLPGNDDIPLPQYMTDQAAGMDIFSAVAEEEIILP
                     GQRKKIATGIAVALPDGYEAQIRPRSGLALKNGVTLLNTPGTIDADYRGEIGLIVINH
                     GEEPFIVTRGMRLAQMVVQRVCRVDWEETETLAKTVRGSGGFGHT"
     gene            10823..11992
                     /locus_tag="PLF77_01560"
     CDS             10823..11992
                     /locus_tag="PLF77_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HPC07570.1"
                     /translation="MKKFKVKKTIKEINEKIKEGRAVVVTAEEMIDVVERHGDVEAAR
                     KIDVVTTGTFGPMCSSGAFLNFGHTSPKIRASRVWLNDVAAYGGIAAVDCYIGATEVA
                     ENDPLNSVYPGEFNYGGGHVIEDLVSGNFVKLRAEGYGTDCYPARKFERNITIKDLRD
                     AILFNPRNAYQNYNCAVNLSDKTIYTYMGMLRPQMANAAYSTSGQLSPLFNDPYYRTI
                     GIGTRIFLGGAQGFVAWPGTQHNPKVLRGSNGVPREGAGTIAVIGNLKEMSADWLTGA
                     SILGYGVSLFVGIGIPIPVLDEEMARYTAVRDEDIYTQVYDYSMDYPKGSPKPLAEVS
                     YKDLRSGTIVLEGKKVITAPLSSYFKARQIAYTLKEWIECGRFILGEPQQNLPSL"
     gene            complement(12084..13424)
                     /gene="holA"
                     /locus_tag="PLF77_01565"
     CDS             complement(12084..13424)
                     /gene="holA"
                     /locus_tag="PLF77_01565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPC07571.1"
                     /translation="MEKTLTNLKKGIVAPCYLLYGEEEYLIKEALNKMIAVLVPEADR
                     DFSLFYLDGENTEINSLMDHLLTPSLLGDTKVIVVQDTTIFTSRDDLAKLAAKIRTTI
                     NENPTKAARYFLTFLNIAGFSLEDLYGTGWQKITDEQWSGLVKGDAGDEREKWLPRIL
                     DICQTAGLTDASTSKTEKLEEVLKAGLPAGNCLIFTAEAVDKRKSLYKIVANIGVVNY
                     FSPVKKEYARKEILQKEAQKLLDGCGKKMAPAAWIALGKKTGFDFRNSLSELEKLISF
                     VGERALIDKEDVEEAVGRTREEEIFALTNALSEKNQLAAISTLNHLLDQGIHQLMIMT
                     MLSREIRLLLQARVMVDSGKLPKFNQSMEYGWFQNTVYPVFSALNPTGRKNDILILNQ
                     HPFSVFNALRNCVRFTTSRLTDLLDELLSLERSMKTSASNPQLLLENFLIKFCA"
     gene            complement(13449..14102)
                     /locus_tag="PLF77_01570"
     CDS             complement(13449..14102)
                     /locus_tag="PLF77_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPC07572.1"
                     /translation="MDEKQYVIDALSVNEAWRVFRIMAEFVEGIETLSNVDNAVTIFG
                     SARVKPDDIYYQKTEKLAKLLVGERFNVITGGGPGIMEAANKGAAEAGGKSVGLNIKL
                     PFEQKPNDYANIHIDYKYFFVRKVMFVKYAVAYVIMPGGFGTMDELFEALTLIQTKKI
                     KSFPLILMGSEYWKGLIDWLNNAMLAEDKILPVDMELIQIVDEPEDVVKLIKKYVIV"
     gene            complement(14160..14351)
                     /gene="rpsU"
                     /locus_tag="PLF77_01575"
     CDS             complement(14160..14351)
                     /gene="rpsU"
                     /locus_tag="PLF77_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HPC07573.1"
                     /translation="MEVKVIDNDVEKALKILKNKLSKSGLFKELKVRRAYEKPSVKQK
                     RKTIEAQRRLAKVQRRRRY"
     gene            complement(14763..15827)
                     /gene="hisC"
                     /locus_tag="PLF77_01580"
     CDS             complement(14763..15827)
                     /gene="hisC"
                     /locus_tag="PLF77_01580"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HPC07574.1"
                     /translation="MIIRNLIAKAILTQNGYSAPEITDAIKLDANENPFTLQEPLKRR
                     LADTLRSIDFNRYPAAGAPDLRERFADYYGVKKNMIMPGNGSDELIQILCMAFRGKIK
                     GVLVPSPTFSMYKIIAVNTGNKVVDVPLDQKFDLDVDAIAARIKNNFPALIFLSSPNS
                     PTGNLFSRERIEAVIRKAPGVVVIDEAYSAFAGQSLLPLLKKYKNVVFLKTLSKLGMA
                     SIRLGFLIGNPDLIDQLDKVRLPYNVNALSQKAAGFFLDHEGEFIKQIKEIIQRRQEL
                     YREIKKIKWIVPYPSRANFIFFSCAFDANRIYKDLVSQGIIVKTLNLAPRMPDGIRVT
                     VGSKKENQAFLDALKNITPE"
     gene            complement(15834..16658)
                     /locus_tag="PLF77_01585"
     CDS             complement(15834..16658)
                     /locus_tag="PLF77_01585"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HPC07575.1"
                     /translation="MGWRFLNYQTNNIFENMAIDEAIFRGVITSKTESTIRFYGSEPE
                     AVSIGYFQDAKKDLNLGQCRHDGIDVVRRITGGRAVFHCNEITYSVTAGSGERIFPDN
                     ISGTYRVISKCLVRGLGNLGIDARLAETGDERNQGEKVACCFSTSAENEILVNGRKIC
                     GSAQVRRRGGFLQHGLLFYRFDPEGTANLLLSSRSPEQIEKLKQSVTSINEYLCQPLT
                     AEEICETLKKSFCDELGIHLGKGSLTPAEEKLKNELMKKYTDGLWNIEREKYFKAV"
     gene            complement(16668..17063)
                     /gene="gcvH"
                     /locus_tag="PLF77_01590"
     CDS             complement(16668..17063)
                     /gene="gcvH"
                     /locus_tag="PLF77_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPC07576.1"
                     /translation="MKDFPEDIIYSCEHIWVKVDGNMATIGITDYAQERLGDILSVEF
                     IETDTYVERDETFGSIESVKEVVDLISPVSGTVVVVNEDILEDVSIINRDPYDTGWLV
                     VIEMDNLEQLEDLLAASNYHDFIAQEESE"
     gene            complement(17083..18168)
                     /locus_tag="PLF77_01595"
     CDS             complement(17083..18168)
                     /locus_tag="PLF77_01595"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HPC07577.1"
                     /translation="MPLNNKKMPQRLSGILEKMSVCKIDGLIICNMSTVRYLCGFTGS
                     EGLLFVGPKKPILFVDGRYTTQAGMETEGIKIIECADRIEAVALMTRQLKLKHIGFEA
                     DFVTWSLHNKLTAQAPETKFIPWTDELKTLRAIKDDDEIMIMKKAAEISSAAIQSVVN
                     RITPGWTEKATAWQLEIAARENGADGIAFETIVASGENSSLPHAKPGKRKIRKGDFVV
                     IDFGVRYQGYCSDETCTLAFGKLTDRQKNAYQVVKDAHDRALELLRADIPGAAIDQHV
                     RSVFGKNYVRYFTHGTGHGVGLEVHEAPRLSSLSDDILKPRMVFTVEPGLYLPGRWGV
                     RIEDTVLLKENSCEKLTKMDKRLTIIE"
     gene            complement(18158..18778)
                     /locus_tag="PLF77_01600"
     CDS             complement(18158..18778)
                     /locus_tag="PLF77_01600"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC07578.1"
                     /translation="MREGNFGEALLIAENHLLDCPADADALGICCEALINMGRLEDMR
                     NILLQTEECISALNLVCERSGDACREKGFHQEAAACYERFISLRPETEKASEIIEKMT
                     LLDQEDHPTVPEKGKRNIPAQDFFTVTLAQLYIDQGHLQNAKTILDEILKKDPDNRQA
                     LSMLNNLISAPAASGTVNGNTKKNDELIKILSSWLKNIERLKMNAA"
     gene            complement(18936..19595)
                     /locus_tag="PLF77_01605"
     CDS             complement(18936..19595)
                     /locus_tag="PLF77_01605"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC07579.1"
                     /translation="MNKGISFLQIGQFNSALKELLEAEKYYSRNPKVHYYLGIAYHGK
                     GMREQAIEEFEKAVSLDKHYSEAHNYLGTLYSEIQLYDKAIREFEKALGNHLYDTPSM
                     ALYNMAWAYYSKKDYKTALDKYQDVLRTDPMTGLRPQVEKNIGLIHYEQNNDMEAIRH
                     LKKSTELDPSLVDAYFILGQSYLRNKDSKNARRAFENVVKLSPDSSFGLKARNYLQSL
                     Q"
     gene            complement(19798..21519)
                     /locus_tag="PLF77_01610"
     CDS             complement(19798..21519)
                     /locus_tag="PLF77_01610"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin and TonB N-terminal domain-containing
                     protein"
                     /protein_id="HPC07580.1"
                     /translation="MKKYFPKIFAAFVIITWIFTCIMGNSFAAFNASEENTAGITMLK
                     SIMLAKLPGKERIHLVVSKQPLIDVKSKDNGSYLIRLEDTDIPESLCRPLGEGELNNI
                     ISIVPSRQQLQGKNWVYLTIYPHEAVPYSIRQEGENVLIDFNVSGIEERKARTISKAS
                     ASKKPLAKVSAEPEKTDDFKKEDLDVSVKKSPAKKYMDRIVSLDFQDADIKSVLRLMA
                     EYGNVSIVTSEEVKGNITLSMKNVPWTKALETILEVNGLTKKQEDNVIIVTSMERSKR
                     EETAREEVRAREQKMMAEKGLLKQVLIEAKIVEATEEFVRNLGVQWGFGTVQRQHIGS
                     SQYGLGASGGSNVATGNQQALTYPSQIGIRDADGNPLAMAAVNLPTSLASPAIGLIFG
                     GASGFLETQLAALETNSTGKIISAPKVVTMEGIKAIIKQGDEVPYITPASGTSPATVS
                     FKEALLKLEVTPKLTDEGKISMDIKASNDTPDWSKAALNPQGNPPIVKSEVESKVVIN
                     DGDTVVVGGILRSTESKAVSGWPWLQKIPVLGWLFKTDSTKRDKRQLLIFVTPKILNN
                     DFTAEGT"
     gene            complement(21534..21911)
                     /locus_tag="PLF77_01615"
     CDS             complement(21534..21911)
                     /locus_tag="PLF77_01615"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HPC07581.1"
                     /translation="MPAPTVPNQADTYSYNPAGKPDPFRPFIVIETVKKTKPEEKKIK
                     PSIFPLQRAEARSYKVVGIVGNEDHRVAIAEDASKKFYPLLVGTRIGLHNGKVVDILP
                     DRVIVEEYENNKGNKVILKLHKN"
     gene            complement(22220..22942)
                     /gene="pilO"
                     /locus_tag="PLF77_01620"
     CDS             complement(22220..22942)
                     /gene="pilO"
                     /locus_tag="PLF77_01620"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HPC07582.1"
                     /translation="MAIDINTIKKIPPKTKVLIVIVGFLLIGYFYWFYFLSSAMEKRT
                     TLNTQLTEMQNKINEQKKIAMQLNKYKADVEALQASYKIALQKLPDQREIPSLFHSVA
                     SAGRDAGVEFVLFEPKASVPKSLESQGPAASKTAALLKPSDQRDQKPADSSEKKDGAA
                     PSTEPFYEEIPVGISVLGSYQNILYFFDKVSKLPRIVNVSDITIGDRKEIKDKGHLVT
                     GSCTIKTYMFIDKKEKTSEKTQ"
     gene            complement(22946..23494)
                     /locus_tag="PLF77_01625"
     CDS             complement(22946..23494)
                     /locus_tag="PLF77_01625"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HPC07583.1"
                     /translation="MIKINLLPYREKAKKDTFLRQIFIVAGSVVLFILLLAWVTIWIK
                     SSISNLETQIKDSEALITDLNKKIGDLEKFKKDKKELEQKIGVIATLEENRLTPLKTL
                     DNLAMLVPPKDIWLVRLTQKGNSLNIEGMGRDNIAVANFMKSIEQFEPVQSVDLVSSK
                     KTEISGNAVQQFNFACILKKGF"
     gene            complement(23491..24573)
                     /gene="pilM"
                     /locus_tag="PLF77_01630"
     CDS             complement(23491..24573)
                     /gene="pilM"
                     /locus_tag="PLF77_01630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HPC07584.1"
                     /translation="MVDLKEIFSGKKSLVGLDIGSSSVKLAEIISNAKGYYLNRFHEV
                     PLPKGIIIDGILADAPVLSSKIKELFKIAHCGRKGVVTSLAGNSVIIKKVTLEQMNET
                     ELRELIHDEAGKYLPFDNMDDVNYDFQILGENNYNPNQMDIVIVAAKKEIVSSYLEAV
                     TAAGQNVEIVDVAPFVLETLYEVNYEFDNEEIVVLINIGASTTGINVVKGGMSVFTRD
                     FALAGNSITESIQEKYQIPFEEAEKLKIEGFPEGSENDNLDLKNNILEFAQPICMEIE
                     RSIDYFRSTFGGEDIKQVLLAGGSSRIPNITKHLSELLNVKTEIMDPFLKINYNKRKI
                     DVKNLDSIKPIAATAIGLGLRKIGDK"
     gene            complement(24609..24878)
                     /locus_tag="PLF77_01635"
     CDS             complement(24609..24878)
                     /locus_tag="PLF77_01635"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HPC07585.1"
                     /translation="MKIVGRLLIIFLLLMSLLITFGNRGLVDNYLMSKRLAELKSQNS
                     AITAENNALKKKIILLRNDINYIEMIARNELGMVKKGDIVYRWAK"
     gene            complement(24933..25130)
                     /locus_tag="PLF77_01640"
     CDS             complement(24933..25130)
                     /locus_tag="PLF77_01640"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPC07586.1"
                     /translation="MADNMKKNHVKKFRETIPLSISELARRAGLTPQTISKMEKGLST
                     RKNSELKVAKALQRPHEEVFP"
     gene            complement(25381..25950)
                     /locus_tag="PLF77_01645"
     CDS             complement(25381..25950)
                     /locus_tag="PLF77_01645"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC07587.1"
                     /translation="MSETTSDWFNKAQALSDGEKFSDPQKAIEYLNNAIRLQPNDAEA
                     YYNRGIAYDNLGRHQQAIKDYSQTVRLKPDYAEAFHNRGTLYNTTGQYQLAIADFNES
                     IRINPGEADNYIGLGFAYDQLGQHQQAITEYSKAIALKPDNARAYNNRGADYLSLGNH
                     QYGCLDAKKACELGNCKLHKIAEKQGVCR"
     gene            complement(26048..26716)
                     /locus_tag="PLF77_01650"
     CDS             complement(26048..26716)
                     /locus_tag="PLF77_01650"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HPC07588.1"
                     /translation="MDLIEAIQHRRSIRAFTDQPVPKETIMEILNIACRAPSAMNTQP
                     WEFIVVSGEPLNKLRAAIVDKLTSGAPITPDHQVVGWPDKSVYKDRQITLAKQLFKAM
                     DIAREDKEKRAWWMERGFRFFDAPAAIIIVSDKILGDHASLMDLGAVMQNICLTALHF
                     GVGTCIEDQGVMYPEVVRHCTGISSNKRLMIAIAVGYPDETFPANHVRSEREPAENLT
                     AWVE"
     gene            complement(26860..26957)
                     /locus_tag="PLF77_01655"
     tRNA            complement(26860..26957)
                     /locus_tag="PLF77_01655"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26920..26922),aa:Sec,seq:tca)
     gene            complement(27038..28276)
                     /locus_tag="PLF77_01660"
     CDS             complement(27038..28276)
                     /locus_tag="PLF77_01660"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HPC07589.1"
                     /translation="MIPVEKALKTILVNFQPLGLETVHLLKARGRVIGENIMSPRNIP
                     SADNSAMDGYAVRCADTRGAGKTTPVKLKIIEDIPAGKFPRKNIGKNETARIMTGAVI
                     PPGADAVIRQEDTRKDGRTVIIFASAARGENIRFAGEDVRKGESVIPKNSLLRPAHIG
                     MLAALGKSSLKVYQKPRVAIMSTGDELVSIRTNPPPGKIVNSNSYSLAAQVAECGAIP
                     LILAIARDKKSDLEKKFKKALNADVIISSGGVSVGDFDFVKNVMGEIGNAMHFWQVAM
                     RPGKPLAFGAIEGVPLFGLPGNPVSAMVSFEQFVRPSLLKMQGHKKIFRQTVTAICHE
                     EFEKAAGFRHFIRAVVRKEKDKYIAVTTGDQGSGILKSMVTANGLIVMGENETRIKKN
                     SQVTVQLLDDSLSWSDALQL"
     gene            28509..32048
                     /gene="mfd"
                     /locus_tag="PLF77_01665"
     CDS             28509..32048
                     /gene="mfd"
                     /locus_tag="PLF77_01665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPC07590.1"
                     /translation="MITRKNPEDMESFRLLLKQIDDGMMRIAVQGLAGAARSFLISLL
                     YKKRQRPVLVVCPEEKEAVAFARDLSLFLGEEHVFHYPALDFLTIDMFALQRDEELSR
                     LEVLANLQIQHKIIVVASANALMQKVMPVAEFSKHLRIIAPGDTINRDEFAAQLVAQC
                     YKRESLVQEKGEFSIRGNIIDIFPPTEKNPLRLEMLGDEIESIRTFNTSSQRSIGPLP
                     AFVLSPAGEAVMDHSTLELAVRNIRRRADELSFSRDIRSRLVAAVQEGLTNAFNPIFL
                     PLFYESYDTGSGLSDNTLSGLLEYLSRNTLVVLDDPLAVGHAMFNAGLAMDKLLFKAK
                     SGGKFYLEKENTYFPPEAVTAGLDGFLQVALAGLEIESERYHVPVVFESDRFTCTGEP
                     LAGEIREDATLRLTVEKIKSWFAGDMLVFLFCPLPEDVQRMKGLLSSYDLPAETLSGA
                     EPVLDSLEGWKGDARLILFVGDISSGFVMPAIKLAVLSEEEIFVKKARRLRSRPVRTG
                     YFIKSFGDLQEGDFVVHTDFGIGIYRGLKKIAVRKIENDFLVIEYSEGDKLYIPVNAL
                     EKIQRYVGPDGYVPKIDRMGGTSWEAVKERVKKSVRDYAEELVSIYAARETLERKSFA
                     PPDRIYEEFCSTFEFEETPDQVKAIEDIHQDMDDVKPMDRLICGDAGFGKTEVAIRSA
                     FRAVMDGKQVAVLTPTTILAEQHYQTFTRRFEDFPVRVGLLNRFKKPAEQKKIVEELK
                     NQTIDIVVGTHRLLQKDVAFQDLGLVIIDEEQRFGVAHKEKLKKMRTLVDVLTLSATP
                     IPRTLHLSLVGIRDLSVINTPPKDRVSIKTYVMEFDEDAIKTAVEKELARQGQVFFVH
                     DRVRSIYAIARLVQRLVPHAKVGVVHGQMKPAEIEKTMAGFIRQENNVLVCTTIIGSG
                     LDISTANTIIINRAEKFGLAQLYQIRGRVGRSSREAFAYLLLPQGAILSRAAMKRLQV
                     IKEFSDPGSGFRIAYNDLEIRGGGSVLGMSQSGHISAVGYELYTELIEKTIREVKGEK
                     TEEDWMPEIQLGISAFIPEDYVPDVHQRLILYKRISLAANDEDIRAIENELLDCYGEM
                     PRVADNLLRVISIRNVLKPLKGKKMGYDGKYLYIFFRENSPVDPGKIIALYRKKIKEL
                     RFTPDHKLFVPAPAAADMDILVQADMLLKMLAQ"
     gene            32072..33079
                     /locus_tag="PLF77_01670"
     CDS             32072..33079
                     /locus_tag="PLF77_01670"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPC07591.1"
                     /translation="MMIPTRWFSKFYSLAGKSKKVSPVRCGLLCLLLPGLMIACDKME
                     SFSRPHVATVNGAKIYLDEYQSLLNEKARMLSGDFFRQPAYVKKFEDEVLDAMIGEKI
                     MLLRARELNLSVSDAELEAKIKEIEKDYGNEFTGLFARQNINYEKWKEDFRKEILLQK
                     LIAADVHSKIIVSETEIEDYFKKHRHIYKRDLSARVSQIVVRDMQTAEQAMQRLKSKE
                     EFARVASEMSIGPEASRGGDLGFITKRVMPEPLDETIFKIPVQTISPIVQSSYGFHII
                     KVMEVRQAKERILPEAREEVIADIRLLKEKTAFAEWLEALKKKAVVQKKADIKINTSY
                     R"
     gene            33101..34075
                     /locus_tag="PLF77_01675"
     CDS             33101..34075
                     /locus_tag="PLF77_01675"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPC07592.1"
                     /translation="MKFYGKYVFALLGIMLLCPLLHAEVVDKIIAVVNDEVITRLELN
                     AAFEPYRKNIENTYQGGDKDQFVLHAREEFLNRLIDNILIEQEAKKTGISASVREEDV
                     MGVLQDMLTKQNISMQDFLKNLAKDGSSIEEVKKEIRSQMIRMRLLRREVKDKIIVTD
                     EEIGAYYNQHRNDYEGKESVRMKQLLLLLPSGANAETKGKIRKEALHLRERIMAGESF
                     DLLVSTYSQGPAAAQGGDVGFIEKGTIIPEVEAVAFSLAVDQVSNVIESDAGFHIIKV
                     VDKRGAGLKPVAVVREEIKAKIEDEKLDKKFEEWISAIRAKSHIEIKN"
     gene            34089..36902
                     /gene="uvrA"
                     /locus_tag="PLF77_01680"
     CDS             34089..36902
                     /gene="uvrA"
                     /locus_tag="PLF77_01680"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPC07593.1"
                     /translation="MDAIKIRGAAQHNLKNISLDIPRDQFVIITGVSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFIGQMDKPNVESIDGLSPAIAIEQRTASHNPRSTVGTVT
                     EIYDYFRLLYAAIGVCHCYRCGREIKSQTIDGILENVLALPENTPFSVLAPMVRGKKG
                     EFQKELKKLQKEGFARVRIDGENRDLAEDISLEKNKRHDIDLVIDRLVLKNGIRKRLR
                     DSLEIASKHAEGLVRIVTAGGEEFLFSEKYACPDCGISLPALTPRMFSFNNPYGACPA
                     CNGLGSSMRFTEYLIVPDAGLSLRDGAIAPWAGRHSLYYYSILDALSSHYGFDVNTPF
                     NKLPEKIRKVLLYGSGSETIKFYHDRGNKRYFVHRPFEGAIRQLERRWRETASTAIRN
                     DLMRYIDLSACETCHGARLKEEILAVTVMGKNIYELCKMSIRDCHSFFERIELSPQEL
                     NITERIIREIKSRLQFLLNVGMDYLNLERATSTLSGGESQRIRLATQIGSGLTGVLYV
                     LDEPTVGLHQKDNLRLIDTLKKLREMGNTVLVVEHDADMIMACDHIVDIGPGAGTEGG
                     EIIFQGTPVQILNSETSLTGRYLSKKESINLPSEKRPLTGRHIILEGASENNLKNIDI
                     KIPVGVFTAVTGVSGSGKSTMVIETLYKVLARRLNQYNGSMGKIKRVVDLGGIERVIM
                     INQHPIGRTPRSNPATYTGVFSFIRDLFTNLPESRMRGYKPGRFSFNVKGGRCEACEG
                     NGLIKIEMHFLPDVYVTCEACHGKRFNRDTLDIKYKDKSIADVLDMTVNQALTFFSNI
                     PSIRSHLQLLGDVGLGYIHLGQSATTLSGGEAQRIKLARELSKKVNSNTLYILDEPTI
                     GLHFADIQKLLDVLMRLVNMGNTMVVIEHNLDVIKCADYVIDLGPEGGSGGGRIISSG
                     TVEKVARDEKSVTGKFLRDVITA"
     gene            37100..38212
                     /locus_tag="PLF77_01685"
     CDS             37100..38212
                     /locus_tag="PLF77_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPC07594.1"
                     /translation="MIVDDEDSILNAFKRILADENYDVHVANSGLEGLNKLRSATKPY
                     SIIISDQRMPEMSGVQFFSKAKEIFPDAIRILLTGYADSDAIIEAINKGGVHLYFTKP
                     WHEEEILLHIKQSLSKVEILAENKRLVELIREKNKELTELNKALEQKALEKTNDLLIQ
                     TEKLKESYQKSQIIMDGIVRTLSKIIETRDPYTAGHEDQVAKIACKIAREMKLPEEQI
                     SFIHIAATLHDIGKICVPSEILTKPSALNNLEKAIIKTHCKVANDVLANIEFPYPVAE
                     VIYQHHERLDGSGYPRGLRGNEIALEARIIGVADVIDAMASYRPYRPALGVDAAIEEI
                     VKYRGITYDPDVVDACLTVYKKTARTLIETNHRTVN"
     gene            complement(38244..38936)
                     /locus_tag="PLF77_01690"
     CDS             complement(38244..38936)
                     /locus_tag="PLF77_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPC07595.1"
                     /translation="MGTDIAANIAAVKQRIAAAAERGKRSPDSVKLLAVTKTVPVPVI
                     EEAIRAGITALGENYVQEAREKIAVIGRSIPWHMIGHLQTNKAKYAVILFDYVHSVDR
                     LDLAAEIDKRAGRAGRQINILIEINVSGEKTKNGIPAGKAVALLQDLSAMQHIAVKGL
                     MTMAPYSDDPETSRPYFRELKNLQQNIIRAGIAGIHMEELSMGMTDDFDVAVEEGATL
                     VRIGRAIFGERN"
     gene            complement(38941..39534)
                     /locus_tag="PLF77_01695"
     CDS             complement(38941..39534)
                     /locus_tag="PLF77_01695"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HPC07596.1"
                     /translation="MSITAHASLILASASPRRQEMLRSAGVHVKIIPAHVNEKYLTGE
                     TPRQHVRRLSSLKAMKVAVENPDACVLGADTIVVIDGTVLGKPRNKTQAKTMLKKLSG
                     RQHTVFTGFTVVRLSSGIRSTGVVQSVVRFKTIHPDEMEWYIAGDEPYDKAGGYALQG
                     QGACFVRSIRGSYTNVIGLPLCEVLETLKKLHVIDFC"
     gene            complement(39961..41037)
                     /locus_tag="PLF77_01700"
     CDS             complement(39961..41037)
                     /locus_tag="PLF77_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPC07597.1"
                     /translation="MYAVIMAGGRGVRFWPRSREKRPKHLLDITGEKTIIQETVNRIK
                     PLVPPTNILVVTGKKHARALMKQLPEIPPRNIMIEPVGRNTAACIGLAALHIRKRSTD
                     DTMMVLPSDHAIADSGKYLSVMKAAAVVAAQEKGLVTIGITPSSVQTGFGYIEGGILC
                     KTIKNQDVFRVKSIREKPDFRTAAKFVRSGNFYWNSGMFVWQTSVILEEIKRWLPELY
                     AGLTLIDTSLGTAGETATVSRVYKKLGSVSIDYGVMEKAGNVFILPGNFGWSDVGSWD
                     ALWDISPKDKQNNVILSGSRTVLEDSKGSLIYNPRKLTALLGVRDLMIVETPDALLIC
                     KKGRSQDVKKIVERLEAGQLKKYL"
     gene            41098..42333
                     /locus_tag="PLF77_01705"
     CDS             41098..42333
                     /locus_tag="PLF77_01705"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HPC07598.1"
                     /translation="MSCNFKAENIFYATMITINYPEIILKKAREDAILRGHPWIFSGA
                     IGSVKGNPGPGDIVLARDAAGHPLALGFFNPSTDIAFRVLTRRCDENISPYFWQSRLH
                     DAYKLRQRLINEQTNAYRLINAEGDGFPGLIVDVYNTTLVFSIAVAGMEKQKNHLLNA
                     LIAQLKPTRIYEQSDSHSRKLEGLENRSGIAFGEDENSTVEIMENGLKFEVDVVSGQK
                     TGFFLDQRVNREKIGSLGRDAHVLNCFAYTAAFSVYCAEGGAKKVVSLDISKTACVAA
                     RKNLHLNWFSVENYPVIETDVFDYFRHTPENYNMIILDPPAFAKTKRDVPKAARGYKD
                     INLQAIKHLSKGGMLATFSCSNFIEEDLFYKIVLGAARDAGAELQLLSRLDAGPDHPV
                     AFGHPEGRYLKGLLLVKRN"
     gene            complement(42357..42755)
                     /locus_tag="PLF77_01710"
     CDS             complement(42357..42755)
                     /locus_tag="PLF77_01710"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HPC07599.1"
                     /translation="MKNTITVFFFIMCLLLAGCGAAVNLQGKVAGISSGRFFYQDGHL
                     VSDYKADIDAVWQACEQSVTDLKAKSIRKERKISTGVITSVIQDEKVTIKVEYVGRDM
                     TSVSVFVGAFGNTMASRLIHDRIIGNLAKN"
     gene            complement(42828..43412)
                     /gene="pyrE"
                     /locus_tag="PLF77_01715"
     CDS             complement(42828..43412)
                     /gene="pyrE"
                     /locus_tag="PLF77_01715"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPC07600.1"
                     /translation="MTIDQMKERLGEIILERSFKYSDNPPFTLASGRQSNYYFNCKPT
                     TLDPEGMNLIGTIIFDMLKDTDITAAGGLTLGADPIANALAVISYQKGKPIKSFIVRK
                     DAKDHGTKSAIEGSVRPGEKVAIIDDVITTGGSTITAIEQARKAGLSVVMVITLVDRE
                     EGGRENIMKHMDNIASILTRTEIMNLRERKAAQR"
     gene            complement(43438..44160)
                     /locus_tag="PLF77_01720"
     CDS             complement(43438..44160)
                     /locus_tag="PLF77_01720"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPC07601.1"
                     /translation="MKYRHSAHQQTALIWALAFSVLIHFSAAAVLIGLELSGGAPAPS
                     DRNVLNMVWVSFDNKKADTGKAVQTGQQTIRQKKNTGRMTSVERPASPVGTVQLPLAS
                     RTAPAVESTFSEYENRPEINKSSVEARHSGGFEAVAQHPMSDIAIAYPLYRENTPPVY
                     PEMARLRGYEGIVLVFAEILPTGRVGDVKIRKSSGYAILDQSAIQAVKPWKFEPAKKA
                     GNPFTAWVELPIKFMLQHHPQS"
     gene            complement(44176..46575)
                     /locus_tag="PLF77_01725"
     CDS             complement(44176..46575)
                     /locus_tag="PLF77_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HPC07602.1"
                     /translation="MVEIINIRNFLKKKIMILDGATGTELQKLGLPAGVCPEIWCLDN
                     PDIIGAVHKSYQKAGAQIIYTCTFGANRYKLKQFGAKHDVYAVNLKLAQLARKACGNQ
                     TLVAGDIGPTGLFVEPFGTLAFEEAVDAFKEQARGLIDGGCDLIVIETMIDIQEARAA
                     LLAVKELADVFTIASMTYEKNGHTLGGTPPQSALITLQSLGADAVGCNCSAGPEQMID
                     FISAMKPYATVPLVAKPNAGMPLLKDGQTVFEMDPATFADFSVKLAAAGANMLGGCCG
                     TTPEHIRALRMSVTRKKPVLPLTKSVCALSSARRFLEIKDNDPLYIVGERINPTGKKS
                     LQQELLEGKLSIIRQMALDQENQGAVLLDINVGQPGIDEVQTIRNVISLLSTVSSLPL
                     VIDSSRIETIEAALRLYPGRMMINSISGEKEKLARLLPLAARYGAMFILLPLTGGEIP
                     RTAKKRRVVIDRIFRKAQAFGFTKKDFVVDGLTMAIASDADAARETLETIQWCKNIFQ
                     CKTILGLSNVSFGMPGRPWINAAFLAMAQHCGLTMAIANPASVEFMNVKNAGDALTAR
                     ERAAFHFINYFSQQTPAVAGLPSTAVSAGEKIVQAILEGNKDDITALLDEAMKSGKTA
                     SQLVDDYMIPSIVRVGDLYEKKIYFLPQLMAAAEAMKKALSHLDPYLQKATITTRGTV
                     LMATVRGDVHDIGKNIVALLLRNYGYRVIDLGKDVSEQAILDAAKKEKPDVIGLSALM
                     TTTMVNMKEVIALARAQGIRTDFLIGGAVVTDQYAKSIGAFYARDGVQAVKVVEKIIK
                     A"
     gene            complement(46678..49296)
                     /locus_tag="PLF77_01730"
     CDS             complement(46678..49296)
                     /locus_tag="PLF77_01730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC07603.1"
                     /translation="MKKNNAPPTGNPQPLQVLMLNDSDKDVSRIVSELQKGGYHPVYE
                     RIETAAAMRQALRDKPWDIILCDSNYLKFGAVAAMALLEEEKTDIPLIVISGASSKDK
                     TIECRFPGTRNYIVKENYSRLCPVIARELNETKIQAKHKQTESQKEAAQEELRRSEER
                     YRKITTCIPDLIWTMDLAGHFTYVNEVVERTHGWTVDEYLKLALQDMVTPQQLAKDTA
                     MMAEQLARSSEPDFDRNTTLTFESEELRKDGTTFLAEVTASFLWSDDGKPIGIIGITR
                     DITERKKAEEALRKSEELHIKLVNAIPDVVVRTDLAGNILFANENALRIGGYSKDEFL
                     GQNLLNFVAPEDRERVIRNLHLMLESRLGPREYHLMTRDGRKILFEINGDVLRKEDGT
                     PFAVVNVCRNITERKQIEKALREKDERLRGITENLPGVIFQFYATQNGKYGITYLSEP
                     IDEFAKILSKFDTDHLDTVFPEFLSRIHEDDRERFLSSITQAAQTASRWNFEGRVAIQ
                     SGKIIWFQGLSVPTRLPDRVIFDGILLNISERKLAEELSHQSEEKFYKIFMTTPDCIA
                     IARLHDGLIIDVNQGFEKIVGWKREQVIGTKSFEPPMNIWLDKTERKAMIADLKTGRD
                     ILDREFKFRRREGSERFGIYSARAITIDEQECVIFILQDITDRKLMDIELKRTLESLR
                     KSVGTTIQVMVSAIEMRDPYTAGHQLRVTDIARAIAAEMRLSQDKIDGLRMAGVIHDI
                     GKLSVPAEILAKPTRLTKLEYSLIQEHSRSGYEILKNVESSWPLAEIVYQHHERMDGS
                     GYPRNLKGNDILIEARILAVADVVESMASHRPYRASLGIEAALEEIEKNKGILYDEHV
                     ADVCLILFRHKGFILA"
     gene            complement(49514..50134)
                     /locus_tag="PLF77_01735"
     CDS             complement(49514..50134)
                     /locus_tag="PLF77_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07604.1"
                     /translation="MESVIFLEKISIEPPREKIYQRLGFKKRITAVSPDQQKQTDLFI
                     REAASLIVLKGMYLRLAVTKNDGKQIFLSNNVHFSSEHLSIFLRECREAVFMGATAGH
                     AVMDAIKAKTRQDDLAAAVVYDATASEMTDAALDWIMNYLNHLLMREGQTLLPRRFSA
                     GYGDFHLENQGIIYKQLHMQNAGVKINPNFILIPEKSVTAIGGICR"
     gene            complement(50168..51274)
                     /gene="leuB"
                     /locus_tag="PLF77_01740"
     CDS             complement(50168..51274)
                     /gene="leuB"
                     /locus_tag="PLF77_01740"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HPC07605.1"
                     /translation="MKNNNFKIAVFAGDGIGQEIINEALKVLKIISGKKNVDFQITEG
                     LVGGTAYDRYGTPLPEESLKLALKSDAVLLGAVGGPQWENLAYSVRPERALLGLRSSL
                     GIYANLRPIVVFEELLDASPLKKDIIRGIDIIIVRELTGDVYFGKPRGIKTSKNGKRK
                     GVNTMIYSEDEIRRIAVRAFEIARTRRKSLLSVDKANILETMELWRNVVTDVGRNYPD
                     VTLRHMYVDNCALQLIRDPSQFDVIVTTNLFGDILSDEAAMLTGSIGMLPSASLGEKT
                     ALYEPIHGSAPDIAGQNIANPLAAILSVALMLRHSLSMPAEAALIENAVTETLQEGLR
                     TRDIDQPGKKIVSTSAMGDAVAGNLNKRLAGIRP"
     gene            complement(51264..51767)
                     /locus_tag="PLF77_01745"
     CDS             complement(51264..51767)
                     /locus_tag="PLF77_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HPC07606.1"
                     /translation="MIFKGKVWKFGDNIDTDAIIPARYLTTSDPEVLAVHCMEDADPD
                     FMKKMKKGDMILGGENFGCGSSREHAPISIKAAGVPCVVAKSFARIFYRNAFNMGLPI
                     FESKDIVDAVKEGNEITVDSARGIITINGTDKFFKINPIPPFMEELIANGGLMQHIAG
                     KRKKNEK"
     gene            complement(51937..53196)
                     /gene="leuC"
                     /locus_tag="PLF77_01750"
     CDS             complement(51937..53196)
                     /gene="leuC"
                     /locus_tag="PLF77_01750"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HPC07607.1"
                     /translation="MGNTITEKILMAHTGLRKISTGDLINARVDIALGNDVTAPIAIN
                     AFKQAGGEKVFNRHKVALVLDHFTPNKDINSAQQCKAVREFASQQKITHFYEGGLVGV
                     EHALLPEQGIVLPGDLVIGADSHTCTYGALGAFATGVGSTDLAAAMLTGELWFKVPPS
                     IKFIIFGKLQKWVSGKDLILHIIGRIGVDGALYKAMEFTGETIARLTMADRFSMANMA
                     IEAGAKNGIFAPDEVTAEYIRKKAKRHYTFYSSDPDAVYADTLEINAATIEPQVAFPH
                     LPSNVKGVSKAGRVKIDQSLIGSCTNGRIEDLRIAAKILKNRKASSNVRLIIVPATPE
                     IYRQALKEGLIEIFLKANAVISPPSCGACLGGHMGILAEGEKAVATTNRNFVGRMGHT
                     KSEVYLANPAVAAASAVLGRIASPEEL"
     gene            complement(53248..54795)
                     /locus_tag="PLF77_01755"
     CDS             complement(53248..54795)
                     /locus_tag="PLF77_01755"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HPC07608.1"
                     /translation="MKKEKNRVYIFDTTLRDGEQSPGYSMNTEEKLMLARQLERLGVD
                     IIEAGFPIASEGDFDAVRQIAKEIKKSQVAGLARANKLDIDRAWQAIKGAAHPRIHTF
                     ISSSDIHLKYQLKKSREQVLKEAVAAVKLARSYTDNVEFSPMDATRSDKDYLVEMVSA
                     VIDAGACTVNIPDTVGYATPEEFGNLIAYLFAHVQNIGEAIIATHCHNDLGLAVANSL
                     AAIRQGARQVECTINGIGERAGNTAMEEVVMALQTRKDLFGLHTNIKTNQIYKTSRLL
                     TQITGIPVQPNKAIVGANAFAHESGIHQDGLIKEKITYEIMTPQSVGISDTHIVLGKH
                     SGRNAISHHLKKMGYQLNDEQINKVAARVKELADIKKDIYDEDLEVILSEEIYRGEDK
                     YSLLYLNVVSGNVAIPTATMEMQVGQDIVRKAGFGNGPVDATFAAIRNITKTNYPLMK
                     YVVNAITGGSDAQGEVTVQLKHSSYSVVGRGAHPDVIVASAKAYINALNRMEFLKNNI
                     KKVKIVE"
     gene            55441..56838
                     /locus_tag="PLF77_01760"
     CDS             55441..56838
                     /locus_tag="PLF77_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HPC07609.1"
                     /translation="MKKFFLLPLAVFFLVAVGLNSFAEEKALTLSASEKLLFNFDFIP
                     VVLTASPDGKRIAYATRVEGKQFVIVDGKAQKRYDGIGSNVVFSPDSKRSAYRAQVGN
                     KQVVVIDGQEGKRYDTVGSNIVFSPDSQRVAYAAKTGKRCFIVVDGKEGKSYEGGIGS
                     NIIFSPDSKKIAYGVLSDKKQFVVVDQQEGTHYDSIGNNIVFSPDSRRMAYQAQSDKK
                     RFLVVDGREGKRYDDVRGDFVFSPDGKQVAYVVKSAGKWQVVVDEQEGKYYDGIGDDI
                     FFSPDSSRIAYQAQSDKKRFLVVNGQEGKGYERIGSTIVFSPDSKKVAYIAQIVNGKF
                     ASVIDGQEGKPYNNIGNGSLVFSPDSKRVAYAAQSGQKRFVVVDGKEGKHYDGVGSYT
                     LVFSPDSTKIVYVAQAENKQIAVVNGKAGEKEVNKYNSIVALGKKGFAFETSDRFRFL
                     AVKEKSVYSIEVKIN"
BASE COUNT        14109 a        13179 c        14561 g        15200 t
ORIGIN      
        1 agcttcccaa gctggacgtc gccggttcga gcccggtcgc ccgctccaat gaaactccaa
       61 tttcgaaagc gtagcgcact gaaatgggta atttgaattg tcccgccaat ccacgacttg
      121 tcgggattgg caggacaaag ggaataataa ggcggataat ttataaatat cattgataaa
      181 aacgacttta atctgcctta atattttact tgctttgtcc atagggatgt gctagcaaaa
      241 acacgaaatc atcgataaag tggtattgat gagtgggccc gtgcccactt ttttattttc
      301 tgagcgatta tggactttga tgttaaagag aaaatccggc ttttagccga gccggttgtt
      361 gcttctgaag gcatggaatt gattcacgtg gaatgcataa agatgcatac gcgctggatt
      421 atccgtcttt ttctggataa ggaaggcggc attacactgg aagattgtac caatataagc
      481 aatcagttag gtgatatttt tgacattcgc gaagtcgtga acggttctta tacgctggag
      541 gtttcgtcgc ccggattcga ccggccgata tcgcgcgatc aggatttttt gaaataccgg
      601 aattcgcgcg tggatgtaaa aacgggcatc aagatcgacg gtgtaaaaaa ttttcacggc
      661 gttttgttgg attatatcga agaaaacggc cggaaactta ttttggtgga agtttcgggc
      721 aaagtttacc ggattccgcg gcaggaaatt ttaaaagcca atcttgccga tgagaagaaa
      781 tagattttta caggaattaa tgccggcagg ctgagattct aatatatcag cgaagaaaaa
      841 agggaggaac ttttaaatgt tgccagaact taaacgtctg atcgagcaga tgggtaagga
      901 cagaggaata agcaaggaaa ttatcgtcga agccctcgaa gcggcaatgc ttactgctgc
      961 cagtaaaaaa ttcggacaga atgtggaaat agaagcccat tataatgatg agaccggaga
     1021 agtggaggtg ttccagttca aaacggtcgt ggacaaggtt ttgcatccgg aaacacaaat
     1081 ttccaaagag gaagcaaggg caacgctcga tgaaggagcg gaacccggcg acagtctagg
     1141 catgaaaata gaaaccagtc agttcggacg gattgccgtt caactggcca agcagatcat
     1201 cattcagaga gtgaaagacg ccgaatgcga caatatctat gatgaataca aggataggag
     1261 aggcgaactg gtcaacggct ttgtccagag atttgaaggc ggcagcattg tcgtcaatct
     1321 cgggcgcgca gaaggcattg tgcctccttc ggaacagatt catcgagaag cttacaaacg
     1381 cggcgaacga atccgcgcct atatcgttga agtaaaaaag agccccaagg gattgcagat
     1441 cattctttcc cgcacccatc cttcattttt gaaggcgctt ttcgaggtgg aggttccgga
     1501 gatttccgaa gggttgattg atattatcaa tgtggcgcgg gaacccggaa aaagaggcaa
     1561 gatcgcggtt aaatccaaag acaaggatat tgatcccgtc ggtgcctgcg tggggatgcg
     1621 ggggtcccgc gtgcaaagcg tagtacagga gttgcgcggc gagaaaatcg atatcattcc
     1681 ctatagcgaa gatccggcca aatatgtcag cagtgctctt tctcccgcta aagtcaaccg
     1741 tatttttatt gatgatgaaa accggaccat gactgtgatt gttccggatg atcagctttc
     1801 tctggctatt ggcaagaacg gccagaatgt ccggctggcg gtcaaactga ccggatggaa
     1861 tattgacatg aagagcgaaa caatggagca ggctcaggag gaaggaaaaa gcggacagaa
     1921 tgatctgacc aagattaccg gcatcggtcc ggccatggcg gaaaaactgt tcagcaaagg
     1981 cataaagagt atcgccatgg tggcggcgac ggaaccggaa ttgttgtctc agattccggg
     2041 gataggcgaa aagacggccc agcagtggat tgaaacggcc ggtaaaattt taaacgagga
     2101 gatcgctggt tccaagagtg attcatcatc tgaaaattaa gaaatttttc atggagattg
     2161 ttgcgcatgc ctaaaaagcg ggtttatgaa ttggccaaag agcttggaat ggagagcaag
     2221 gttctgatcg ctcgtttgga aaaaatcggc atttcggtta aatcggcgtc ggcttctctg
     2281 gaagaggctg atgcggagcg ggccaagaaa gagctgaccg ccggagaact tcgtgaagtc
     2341 gtggaacaga ggatcaagac cacggttatc agacgccgca cggtcgtgac cgctgtggag
     2401 aaaccgcctg aagaagtccc ccttgaggaa gccaaacctc cggaaaaaga acctgccgaa
     2461 gacaagctta aaaaagcgcc tccgggaaaa actgaaaaag ttaaaaagga aaagcctggg
     2521 gaagagcctg ctgttcctgc agagacggtt tcggaaaaag cggtgaagga ggtcgttcct
     2581 ccggcagcgg ttggaccggc tgccgccgaa acgaaggctt ctttggaaca gaaagccgcg
     2641 ccccttgaaa agtcggcaga ggaaaaagct gtcaaaccgg aaacaaagga agcgccggtt
     2701 gctcctgtca agccgacgat cattaccaaa cataaaatta tcaggccgga aacaggaaag
     2761 gcggttcccc ccaaagtgcc ggctgccaaa ccggcggaaa aaatatcaca accgtctgcg
     2821 gaaaagccga agaaaaaagg caaatatcag gtcgaggtct tgatcgaaga agagaagaag
     2881 atcccgacca agaaaatcct ggaaaagaag atcgaaaagc ggctcaaacg gcatgaagag
     2941 gatcaggaag ttgtttttac caaatggcgt gaggggaaaa aggccgcgcc gaccaagatg
     3001 aagaaaacgg agattaccgt tcccaaggcc attaaacgac ggatcaaaat cggcgaaacg
     3061 attaccgttg gcgaactggc caaacgcatg ggcatcaagg tcagcgagat tatcaataag
     3121 ctgatgtcca tgggcgtgat ggcgaccatc aatcaggcca tcgattacga taccgccagc
     3181 attgttgcgg gtgaattcag tttccagctg gaatccgcgg acgtggaatt tgacgaatcc
     3241 gttctgaagg ctccctccac accggagaat ctcaaaccgc gtgctccggt ggtgaccatc
     3301 atgggacatg tcgatcacgg caaaacctcc ctgctggatg ccatccggca gaccaatgtt
     3361 tccgacggtg aaacgggagg catcacgcag gcgatcggcg cctatcatgt gcatgtcaac
     3421 ggccgggata ttgtgttcct ggataccccc ggtcacgagg cgtttacagc gatgcgggcc
     3481 cgcggagcgc aggttaccga tatcgttgtt ctgatcgtgg ccgccgatga cggcgtcatg
     3541 aatcagaccg tcgaggccat caatcatgcc agggttgccg gcgttccgat tatcgtggcg
     3601 attaataaaa tcgacaagcc gggtgcggat cctgaaaaaa taaaacaagc gttgaccgaa
     3661 cataatttgc tttccgaaca gtggggcggg gatacgattt tctgtgaagt atccgccaag
     3721 aaaaaaatca acattgaaga actgctggaa atgattctcc tgcaggccga tgtcatggag
     3781 ctgaaggccg atcccgatcg ccctgttcgc ggcattatca tagaatccaa actggataag
     3841 ggacgcggtc cggttgcgac cgtcctgatt caggaaggca cgctcaggga aagcgattcg
     3901 tttgtttcca agaccgaatt cggccgtgtt cgcgcgctga ttaatgatca gggacggcga
     3961 atcaaggaag ccggaccgtc catgccggtg gaagtgatcg gattttccag tgtgccgcag
     4021 acgggagcgg aatttttctg cgtcgacgat gagaagaaag cgcgtgcgat tgccgattac
     4081 tggaccagaa aggcccggga aaaggaactt tcgtctctgt ccaagatcac cctggaacag
     4141 ctttatcaga ggataaaaga aggggcgaag gaattcaacg ttattgtcaa ggccgatgtg
     4201 caggggtcga tagaagcgat ttccgacgca ttgaataaac tttcaacgga cgatatcaag
     4261 ctgaagatta ttcatagttc caccggggcc atcagcgaaa cggacgtgat gcttgcgtcg
     4321 gcttcccagg ctattattat cggctttaat gtgcgacccg atgcgcgggt ggtggatgtt
     4381 gcccagcggg aaggagtgga aatcaagctc tacgatatca tttataatgt gattgcggat
     4441 gtgcgggcgg ccatggaagg tcttctggaa cctgaatacc gggaggttgt tcagggcaga
     4501 gcggaagtgc gcgaactttt caaggtgccg aaggtcggta ccattgccgg gtgttatgtc
     4561 acggatggaa aaatcatgcg cagcaataat ctcaagcttg tccgtgacag cgtggtcgtt
     4621 tttgacggaa agattctgtc attgaaacgt tttaaggatg acgcccggga agtgctggcc
     4681 ggtttcgaat gcggtatcgg catcgaagga tttaatgata ttcatccggg agatgtcatt
     4741 gaggcgtatg cgatagaaac gttggaaaag aaactggaac cgaatgctga taagcgatag
     4801 tttttttata tccgaacggt tctaaaaaaa tcggggctcc cgaatgcttc aggtttccgg
     4861 agaatgctta tggttgtcgg atacggcatc attaatctca gaattccgga aagcggctct
     4921 ttaaaaagca agcgaagcgt tttgaaccga attttaaaac gcgcacaaaa tgaatttaat
     4981 gtttccattg cggaaacagg ccatcttgat aattataagc tggccgaaat ttcttttgcg
     5041 gttgtcggca acgattcccg ttatatcaac gggaaggcgg atcatctgct gcgttttatc
     5101 gatcatctgc agattgccga gattgtaaac tgcaaaatgg aaattatggt tgtttcgagt
     5161 tttctggaaa cagccgacgg ggatatacag aagtacgatg aattttaaaa gagcggaccg
     5221 ggtcgccgaa ttaatccggg cggatatgtc cgaaataatc atgaaggacg tgaaagaccc
     5281 gcgtctgcat tcggtaacca ttacggcggt caaggtgtcg gatgatttgc gcaatgccaa
     5341 gatttatttc gtggaaatgg gcaaagacga atgctctgtt gaaattaaaa cgggactggc
     5401 tcaggcaagc ggattcatac gccgggagct gggcaagaaa ttgcagttgc gttttgtgcc
     5461 ggaactgatg tttattcatg atacgtcttt cggttacggg aaccgtatcg aaaaattgtt
     5521 ggcacagatc cacagtcagg aaaatgaaaa tgattcatga tattatccaa gcgattcacg
     5581 aaggacagaa ctttctgatt accgcccatg tgcggctgga cggagatgcg ctcggttcgg
     5641 aacttgccgt ttatctgatg ctgaaagaac tgggcaagaa ggccgttgtc tataacaagg
     5701 atcgcacgcc ggaacgttat cagtttctgc cggccacgca caacatttct catacgttaa
     5761 cgaatatcga gcagtacgat acctgcatcg tgctggattg cagcgatttg tcgagggtcg
     5821 gcgatgaagc ggaaaatatc agaaaaatca agacgctcat taacatcgat caccatgttt
     5881 ccaataacgg attttgtgct ttaaaaatgc tggatgccaa agccagttcc accggcgaac
     5941 tcgttttccg cctgatgcag gacatgcgct tcaaaatgtc cagagacatc tgcaccaatc
     6001 tgtatgcggc catcattacc gataccggca gtttccgcta ttcgagcacg acaaaggaga
     6061 cgttcatggc ggcgggggtg ctggtggggg aaggggcgag tccccagcgc attgcggaaa
     6121 atatttatga aagcgattct ccggcccggc tcaaactgat ggcccgggca ctgtcgacgt
     6181 tatcgcttga tctggaaagc aaggtcgggt ccatggtcgt tacccagaaa gatttgctgg
     6241 aaacgggcgc cgcctgggag cacacggaag gatttgttga tataccccgc acggtgaagg
     6301 gtatcgaagt atcggttctc tatacccaga gaggggaaaa aaatttcaag ctgagtttgc
     6361 gttccaaagc caaggtcgac gtggaaaaag tggccaaacg gttcggcggc ggcggccata
     6421 ttcatgcctc gtcctgctgg atggacggcg atattgaaac catcaaatcg caaattattc
     6481 aggcggtcag ggaattgtag tgttaatgat gaacggcgtt attgtgatcg ataaaccggc
     6541 ggggaaaacc tcgcacgatg ttgttctgga tgtgagaaga gctctgggga taaaaaaagc
     6601 cggccataca ggaacgctgg atcccctggc tacgggcgtg ctgcctgttt gcgtgaatga
     6661 agccaccaaa ctggccggtt ttctggcggg cgacaaaaaa gaataccggg ccacgatgtt
     6721 gctgggtgtg acaacggaca ccctggatat tgagggaaac attacgggac aatccgataa
     6781 ggttgtgtct ggggaagaaa ttcgatcggt catgatgcgg atgatcggaa ccataaacca
     6841 ggtcccgccg gcgtattccg cggttaaaca ttgcggcaag cccctgtata aatgggcaag
     6901 aaaaggggtt ttcccggatt tgaagccgag ggaaatcaaa attttcagcc tgcttgtgga
     6961 gaatatttct tttccgtcgg tgaagttccg tgtggcctgt tcaaaaggga cgtatatccg
     7021 gtctctttgt gcggatatcg gggaagcgct gggaacaggg gcctgtctga gcgggctgcg
     7081 ccgtttgcaa agcgggattt tttcagaggc gatggccgtt aatctaaatg attataagtt
     7141 cccggaagca aaagagaaac tggctgaaaa aatattgccg atggctgagc ttctgccgct
     7201 tctggccacc attgaactgg aagatcaatg cgcggtaaaa ctgcgcaacg gctggcagcc
     7261 ttcggtggaa atgctcaagg agcacgatct tcccttgctt aaagcaggag atatgataaa
     7321 attcgtcagt cgcggcaatc ttctggctgt tgcggaaatg ctggccccgg tcagcgagct
     7381 ggctgctttt gacggaaaaa ggcaggcggc cagaattctc cgggtgttta atcagccgtg
     7441 aatgaagaac ataaaaacca acataaaaga ttataaattg taaaaaggag gaaaacgcgt
     7501 gttaagttcg gaaaagagga aagaagtcat tgataattat cggctgcatg aaaaggatac
     7561 ggggtctccc gaggtccaga tcgctctttt gagcgccaga atcgagtatt tgacggagca
     7621 tttcaaggcg cataagaaag atcatcattc gcgccgaggt ctgctgaagc tggtcggcca
     7681 gagaagaagg cttctcaatt atatcaaaga aaatgacatc gagagataca gaaccgttat
     7741 taaacgtttg aatctcagaa agtaaaagaa ttattcaagg gcggaaggca taaggcaagc
     7801 tgaccgtaag cgaatcgatc gttgaacggt tgttgcttta tgcgtttcgc ccttgaattt
     7861 gaaaagacca gcactcacaa acaattatgg aggaaaatga atgagtaatg tattcagtgc
     7921 ggatttcgcc gggcggaaca tttccctgaa agcgaattat gtggccgcgc aggccgatgg
     7981 ggccgttttg gtttactacg gcgacacagt tgtattggtt acagcggttt ctctgaaaag
     8041 cacgcgggaa ggcgtggatt ttctgccgct gacggttgat tatcaggaaa tgacttttgc
     8101 cgcaggaaaa attcccggcg gcttttttaa acgggaagga cgtcccaacg aaagggaaat
     8161 tctgacatcg cgcgttattg atcgcgcgat tcgtccatta ttccccaagg gatatttttc
     8221 tgaaacacag ttggtggcca cgcttttatc cgtggacaaa gaaaacgatc ccgatgttgc
     8281 gtccctgatc ggcgcttccg ccgctctggg gatatctgac attcctttca aggggccgat
     8341 cgccggcgtg cgtgtcggcc ggataaacgg cgaatttgtc gccaatatgt gcccggagaa
     8401 gatgaaggaa agcgagatga gcctttttct ggtgggacga aaagtgacac ccggaacgtc
     8461 cggccgtccc tatgacgtgg aactggtcat gatggaaggt gaagccaaag aggttccgga
     8521 agatatcatc gtcgatgcta ttaaattcgg gctggaatcg atccggccgg ttattgacct
     8581 gcaggatcag attcaggcat ctatcggcaa ggttaaaagg ccggttgagg agccggttcc
     8641 ggatgaagag cttgttgctc gcgtgcgtga agaagccctg cccggcttga aagaggggta
     8701 tgccatgccg cgcaagctgg aacgttacag caaactgggc gacgttcgcg ccgaggttat
     8761 aaaaaatatc ggcggagacg acgccgtcct gagtaaaaaa gttgccggca ttatcgagaa
     8821 cctggaatcc cgcatcctgc gcgatatgat cattcaggac aagaaaagaa ttgatggccg
     8881 ctcttccgtt gatattcgtc cgatttcttc cgaggccggt gtgctgccgc gcgcccacgg
     8941 ttccgcactg ttcagccggg gggaaacaca ggcgctggcg gcgctgacgc tggggacatc
     9001 cgccgatgag cagcggatgg attacgtcgg gggagaagag atccggacat ttttattgca
     9061 ctacaacttt cctcccttca gcgtggggga ggccaagatg ctgcgcagtc ccggacggag
     9121 ggaaatagga cacggagcgc tggcgcgcaa agctctggtg ccggttctgc cgtctccgga
     9181 ggaatttccc tacaccatcc ggattgtttc ggaaattttg tcgtccaacg gttcgtcatc
     9241 tatggccaca gtctgcggcg gtattttatg cctgatggac ggtggggtgc ccgtgaaaga
     9301 tatcgtggcg ggcattgcca tggggctcct caaggaaggc aatgagatcg ttattctgtc
     9361 cgacattctg ggcgatgaag atcatgccgg tgatatggac ttcaaggtat gcggtacgga
     9421 aaagggcgta accgccatgc agatggacat caagattgac ggcttgaccg aggatattct
     9481 gcgcaaggcg ctggctcagg ccagagaggg acgcatgcac atcatcggta agattcgtga
     9541 gacgctggct gcgccgagag gcgacatttc tcaatatgcg ccccgtatca cgacggtgaa
     9601 ggtcaaggaa gatcaggtgc gcaccgttat tggttccggc ggcaagaaca tccgtcagat
     9661 tatcagtgag accggtgtga cgattgatgt ggaagatgac ggaaccgtca ccattgcctc
     9721 cgccgatgcc gaggcagccg cccgtgcggt ggccatggtg aaatggctga cggaagaagc
     9781 cgagattgga aaaatttacc gcggcaccgt caagaagatt gtcgatttcg gcgcgtttgt
     9841 ggaaattctg cccggaacgg aaggcctgtt gcatatttca cagatcgcca aggaaagaat
     9901 cgccaaggta accgatgttt tacaggaagg cgatgaagtg atggtcaagg tgctggaaat
     9961 cgacaagtcc ggcaagatcc gcctcagccg caaagaagca ctgggcgcgg aagtnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcgaca agtccggcaa gatccgcctc
    10141 agccgcaaag aagcactggg cgcggaagtc aagtaataca ttcccggtta agggttatgg
    10201 aaaaacaagc cgttccgcat gcaacgatac gttggtgcgg aacggatctc tttatcaacc
    10261 attgtaagca gttctcccaa taattatttc tggtcgaaac atgaaaaaag tcaaggtttg
    10321 tattcagaga cttccgggaa acgatgatat ccccctgccg cagtacatga ccgatcaagc
    10381 cgccggcatg gatatttttt cggcggtggc tgaagaagaa attattttgc cgggccaaag
    10441 aaaaaaaatc gcaacaggca tcgccgtcgc tctgcccgat ggctatgaag cgcagatcag
    10501 gccgcgcagc ggactggcgc tgaaaaacgg cgttaccctg ttaaatacac cggggacgat
    10561 tgacgccgat taccggggag aaatcggtct gattgtgatt aatcatgggg aagagccctt
    10621 tatcgtcaca cgaggcatgc gtctggcaca gatggtggtg cagcgcgtct gccgggtgga
    10681 ttgggaggaa accgaaacac tggcaaaaac agtccggggc agcggcgggt tcggccatac
    10741 ctgaacgctt cttagctttt gcaattggaa acaaagagtg gtatgaaaat ggacaatgac
    10801 gatgtgacga taacgatacg gtatgaaaaa attcaaagtt aagaaaacaa taaaagaaat
    10861 taacgaaaag atcaaagaag gacgtgctgt cgtggtgaca gcagaggaaa tgatcgacgt
    10921 tgtcgaacgc cacggcgatg tggaggccgc ccgcaagata gatgttgtga ccaccggaac
    10981 gttcggaccg atgtgctcct cgggggcgtt tctcaatttc ggacatacgt ctcccaagat
    11041 cagggcttcc cgggtgtggt tgaatgacgt cgccgcttac ggcggtattg ccgccgtgga
    11101 ttgttatatc ggcgcgacgg aagtggccga aaacgatccg ctcaacagtg tttatcccgg
    11161 tgaatttaat tacggcggcg gtcacgtcat cgaggacctg gtttccggaa attttgtcaa
    11221 gcttcgtgcc gaagggtacg gaaccgattg ctatcccgcc cggaaattcg aaagaaacat
    11281 tacgataaaa gatttgaggg atgcgattct gttcaatccc cgcaatgcgt atcagaatta
    11341 taattgtgcc gtgaatctgt ccgacaaaac gatctacact tacatgggga tgctgcgtcc
    11401 gcaaatggcc aatgcggcat attccacgtc cggacaatta agtccattat ttaatgatcc
    11461 ttattacaga accatcggga tcggtacgcg catatttttg ggtggcgcac agggatttgt
    11521 cgcctggccg ggcacgcagc ataatccgaa ggtgctgcgc ggatccaacg gtgttccccg
    11581 ggagggagcc gggacgattg ccgtgatcgg taatcttaaa gaaatgtctg cggattggct
    11641 cacgggtgcc agtatcttgg gttatggggt ttctcttttt gtgggcattg gtattcccat
    11701 tcctgtgttg gatgaagaga tggcccgtta cacggccgtc agagacgagg atatctatac
    11761 ccaggtatac gattacagca tggattaccc caagggcagt ccaaaacctc tggcagaggt
    11821 cagttacaag gatctgcgca gcggcaccat tgttttggaa ggaaaaaaag tgattacggc
    11881 gcccctttcc agttacttca aagccagaca gatcgcctat acgttaaagg aatggattga
    11941 gtgcggccgg tttattctgg gcgaaccgca acaaaatctg ccgtcgttgt agaatcacga
    12001 gagtgccggc gacgcgaaaa ccttttcgca cgggcatctc aaatcgttgg atcgagcagt
    12061 gtaccggcat tttccggtaa aactcaggca caaaatttaa tcaaaaaatt ttcgagaagc
    12121 agttgcgggt tggaggcgga tgttttcatt gaacgctcga ggctgaggag ttcatccagc
    12181 aggtcggtca ggcgggaggt tgtaaagcgg acgcaattac gcagtgcgtt aaaaacggaa
    12241 aagggatgct ggtttaaaat caggatatcg ttttttctgc cggtgggatt caacgcgctg
    12301 aagaccggat aaacagtgtt ctgaaaccag ccgtattcca tggattggtt gaatttcggc
    12361 agtttcccgg aatcgaccat gactctggcc tgcagcagga gccggatttc cctggatagc
    12421 atggtcatga tcattaattg atggatgccc tgatccagaa gatgatttaa ggtgctgatt
    12481 gcggccagtt gatttttctc gctcagagca ttggtcagag cgaaaatttc ctcctccctt
    12541 gttctgccca ccgcttcttc cacatcctct ttatcgatga gcgcccgttc tccgacgaag
    12601 gaaataagct tctcaagttc cgacagagag ttacggaaat cgaaaccggt ttttttgccc
    12661 agggcaatcc atgccgccgg agccattttt ttcccgcagc catccaatag tttttgcgct
    12721 tctttctgaa ggatttcttt gcgtgcgtac tccttcttga ccgggctgaa atagttgacg
    12781 acgccgatat ttgcgacgat tttatagagt gatttccttt tatccacggc ttctgcggtg
    12841 aaaatcaggc agttgcccgc gggcaatccg gctttcagaa cctcttccag tttttccgtt
    12901 ttactggtgg aagcgtccgt cagtccggcc gtctggcata tatccagaat tcggggcagc
    12961 cacttttccc gctcgtcgcc ggcatctccc ttgacaaggc cgctccactg ttcatccgta
    13021 attttctgcc agcccgtacc ataaagatcc tccagggaaa atcctgcaat attgaggaag
    13081 gttagaaaat atctggccgc tttggtcggg ttttcgttga tggtggtacg tattttggct
    13141 gcgagtttgg ccagatcatc acgcgatgtg aaaattgtgg tgtcttgcac gacgatgacc
    13201 tttgtgtcgc ccagaagaga aggggtcagg agatgatcca tcaggctgtt tatctctgtg
    13261 ttctcgccat ccagataaaa cagactgaaa tcccgatcgg cctccggaac aagaacggca
    13321 atcattttgt tgagggcttc tttgatgaga tattcttctt caccgtaaag aagataacag
    13381 ggtgcaacaa tgcccttttt cagattggtt aaggtttttt ccatgacgca tcaggggctc
    13441 aaggacgatt atacgataac gtatttttta atgagtttga caacatcttc cggttcatca
    13501 acgatctgaa ttaattccat atcaacggga agaattttgt cttccgccag catcgcattg
    13561 ttcagccagt cgatcaggcc tttccagtat tcgcttccca tgagaatcag agggaaagac
    13621 ttgatttttt ttgtctggat gagcgtcagg gcctcgaaaa gctcatccat ggtgccgaag
    13681 ccgcccggca taatgacgta ggcaaccgca tatttgacga acatgacttt gcggacaaag
    13741 aaatatttgt aatcaatgtg gatgttcgcg taatcgtttg gtttttgttc aaagggcagc
    13801 ttaatgttca atcccacgga tttccccccg gcttctgcgg cgcctttgtt tgccgcttcc
    13861 atgattcccg gtccgccgcc ggtgatgacg ttgaatcttt ccccaaccag gagttttgcc
    13921 agcttttcgg ttttctggta gtagatgtcg tcgggtttga ctctggcaga accgaaaatg
    13981 gtgaccgcat tgtcgacatt ggagagcgtt tcaatcccct cgacgaattc cgccatgata
    14041 cggaaaaccc gccatgcttc gttgacggac agagcgtcaa tgacatattg cttttcatcc
    14101 atagatcgac accttccctt cgagcggtaa tggtatggaa taggaataaa aaaatgagtt
    14161 taataacgac ggcgtctctg aaccttggcc aggcggcgct gagcttctat ggtttttctt
    14221 ttttgtttta cagacggctt ttcatacgcc cggcgaactt tcagttcctt gaacaagccg
    14281 cttttcgaaa gtttgttctt caagattttc agtgcttttt ccacgtcatt gtcgattact
    14341 tttacttcca aatttattcc tccctgttaa tagttgttag tgatcgtgtg acttctgtca
    14401 acgcttattc atcttgtccg atacgtcccg ggttggcgga ttgtgtccgg ttgcctgcgc
    14461 cgatcggata atttgactta caaatttcat tgcgcttcgc tgtgcttgtt cttggcgagt
    14521 gtcacatccc gtttcgttcc gcccctccgg aggcattcga tcagcaagtg tcggtgcact
    14581 tcgtttctga aaattggtct caaattccac gtcgcgacga tcctgggata attcgactaa
    14641 caccacaaac ggcactgcgt ttattttcac tgagtttcat aacaaaaaaa gggcagtacg
    14701 gcaaagcgaa aaagctgccg taatccaccc tgcaatgtcc cactaatttt aaatcacgct
    14761 ccttattctg gagtaatgtt ttttaaggcg tcgagaaagg cttgattttc ctttttactg
    14821 ccaaccgtta ctctgatgcc gtcaggcatc cgcggcgcaa gattcagcgt cttgacgatt
    14881 atgccttgcg aaacgaggtc tttatatatg cgattggcat caaaagcgca actaaaaaaa
    14941 ataaaatttg cccgggaagg gtatgggaca atccatttga ttttttttat ttcccggtag
    15001 agttcctggc gtctctgaat tatctctttg atttgtttga tgaattctcc ttcatgatcg
    15061 agaaaaaatc ccgcggcttt ctgagacaga gcattgacat tatagggtaa tcttaccttg
    15121 tccaactgat cgataaggtc gggattcccg attaaaaagc ccagacgtat ggaagccatg
    15181 cccaatttgg aaagcgtctt tagaaaaaca acatttttat attttttcaa cagcggaagc
    15241 aggctttgtc cggcaaaagc gctataggct tcgtcaatga cgacaacgcc gggggctttt
    15301 ctgataacgg cttctattct ttcgcggctg aaaaggtttc cggtggggct gttgggtgaa
    15361 ctcaggaaaa tcaacgcggg aaaattattt tttattctgg cggcgatggc atccacatcc
    15421 agatcgaact tttgatccag aggaacgtcc accactttat taccggtatt gaccgcgatg
    15481 attttgtaca tggaaaatgt tggagaagga accagcacgc ctttgatttt gcccctgaag
    15541 gccatgcaga gaatctggat cagttcgtcg gagccgttgc ccggcatgat catatttttt
    15601 tttacgccgt aatagtcggc aaacctttcg cgtaaatccg gagcgccggc ggcagggtaa
    15661 cggttgaaat caatcgaacg cagcgtgtct gccagtctcc tttttaacgg ttcctggagg
    15721 gtaaaaggat tttcattagc atccagtttg atcgcatcgg tgatttccgg cgccgaataa
    15781 ccgttttgtg tcaatattgc ttttgctata agattccgaa tgatcatgtt gaatcaaacc
    15841 gccttaaagt atttttcccg ctcgatattc cacaagccat ctgtatattt tttcatcaat
    15901 tcgtttttga gtttttcctc cgcgggcgtc agagatcctt ttcccagatg tattcccagt
    15961 tcgtcgcaga aacttttttt taatgtttcg catatttctt ccgctgtaag cggttgacac
    16021 agatattcgt ttatcgacgt cacggattgt tttaattttt cgatttgctc cggagaacgg
    16081 gaggaaagca ggagattcgc cgttccctcc ggatcaaacc tgtaaaaaag aagcccgtgc
    16141 tgtaaaaatc ctccccgtct tctgacctgc gcgctgccgc agatcttgcg gccgttcacc
    16201 agaatttcat tctctgccga tgttgaaaaa cagcaggcaa ctttttctcc ctggttcctt
    16261 tcatcgccgg tttcagccaa gcgggcatca atgcccagat tgccaagccc gcggacgaga
    16321 catttgctga tgaccctgta tgttcctgat atgttgtcgg gaaaaattct ttctccgctt
    16381 cccgccgtta cggagtatgt gatttcattg cagtgaaaaa cggcccgccc gcctgttatt
    16441 ctccgaacaa catcaatccc atcgtggcgg cattgcccga ggtttaaatc ctttttcgca
    16501 tcctgaaaat aaccgatgga aacagcttcc ggctctgatc cgtaaaatct tatcgtggat
    16561 tctgttttac tggttatgac tccgcgaaaa attgcttcat cgatggccat gttctcaaaa
    16621 atgttattcg tttggtaatt gagaaatctc cagcccataa agaagaatta ttcgctttct
    16681 tcctgcgcta taaaatcatg gtaattggac gctgccagaa ggtcttccag ttgttcgaga
    16741 ttgtccattt caatcacgac cagccatcct gtgtcataag gatctctgtt aatgatactg
    16801 acatcttcaa gaatatcttc attgacaacg acgacagtgc cgctgacagg tgaaattaaa
    16861 tccaccactt ctttcacgga ctcaattgag ccgaatgttt catcgcgctc cacataggta
    16921 tctgtttcga taaactcgac ggaaagaata tcccccaatc tttcctgtgc gtaatcggtg
    16981 atgcctatgg tggccatatt tccgtcaacc ttcacccaga tgtgttcgca actgtaaatt
    17041 atatcttccg gaaaatcctt catgatagaa caacctcttt ctttattcta taatggtaag
    17101 ccttttatcc atttttgtca gcttttcgca actgttttct ttcagtaaca cggtatcttc
    17161 aattctgact ccccagcgtc ccggaagata taatccgggt tccacggtaa ataccattcg
    17221 cggttttaaa atatcgtctg aaagggaaga caggcgcggt gcctcgtgaa cttccagacc
    17281 gacaccatgg cccgtgccat gcgtaaaata ccggacataa tttttcccga agacactgcg
    17341 gacgtgttga tcgattgccg ccccgggaat gtctgccctg agtaattcca aagcgcgatc
    17401 atgagcgtct ttcaccacct gataagcatt tttttgtctg tctgtcaatt tcccaaaagc
    17461 aagggtgcag gtttcatccg aacaatagcc ctgatatctc acgccgaaat caatgacgac
    17521 aaaatccccc ttcctgattt ttcttttgcc cggttttgcg tggggcaggg aagaattttc
    17581 accggaggca acaatggttt caaaggcgat gccgtcggcg ccgttttccc gggcggcgat
    17641 ttccagttgc caggccgttg ccttttccgt ccagccgggc gtgatcctgt tgacgaccga
    17701 ttggatggcg gctgaagaaa tctccgccgc ttttttcatg atcattattt catcatcgtc
    17761 tttgatcgcc cggagtgttt ttaattcatc ggtccagggt atgaattttg tttcaggagc
    17821 ctgagcggtt aatttattat ggaggctcca ggtgacaaaa tcagcttcaa aaccgatgtg
    17881 ttttaatttc agttgcctgg tcataagcgc aactgcttcg attctatcag cgcattctat
    17941 gattttgatt ccttccgttt ccattcccgc ctgagtggtg taacggccat cgacaaacaa
    18001 aatgggcttt ttagggccga cgaagagaag cccttccgag ccggtgaaac cgcacagata
    18061 gcggacagtg ctcatattgc aaatgatcag accgtcgatt ttacaaacgg acattttttc
    18121 cagaattccg gaaagtcttt gcggcatttt tttattatta agcggcattc attttcaatc
    18181 tttcaatatt tttcagccag gaagataaaa tcttgatcag ctcgtcgttt ttctttgtgt
    18241 tgccgtttac ggttcccgat gcagccgggg cggatatgag gttgttgagc atggacaggg
    18301 cctgccggtt atcggggtct tttttgagaa tttcgtctaa aatggttttt gcgttctgaa
    18361 gatgtccctg atcaatgtat aactgagcca gggtaactgt aaaaaaatcc tgtgcaggaa
    18421 tatttctttt acccttttcc gggacggtgg gatggtcttc ctgatccaac aaggtcattt
    18481 tttcaatgat ttcagacgct ttttccgttt ccggccgcaa agagatgaac ctttcgtagc
    18541 aggcggcagc ttcctgatga aaaccttttt cacggcaggc gtcgccggaa cgctcacaaa
    18601 caagatttaa cgcggaaata cattcttccg tttgaagaag tatgttacgc atgtcttcca
    18661 gtcttcccat gttaataagc gcttcgcagc agatgcccag ggcatcggca tctgcgggac
    18721 agtccagcaa gtgattttcg gcgataagga gcgcttcccc gaaatttcct tcccgcaaaa
    18781 atgcttctgc tttgcgtaaa aggagatttt gttttttgtc aaatctgcta ttttccatgt
    18841 ttaatcaaat aattaaatca atgtgcaatt tggcgttacg taacacagca catggggagt
    18901 gtcaagaatg gaaaagacag gatgttttac acagattact gtaatgattg caggtaattt
    18961 ctggctttca atccgaaaga agaatcgggg gacaacttga cgacattttc gaatgcccgg
    19021 cgtgcgtttt tgctgtcttt gtttctcagg tagctctggc ctaaaataaa gtaggcatcc
    19081 acaagcgatg gatccagctc ggtcgatttt tttaaatgcc tgatggcttc catgtcattg
    19141 ttttgttcat aatggataag gccgatattt ttttctacct gcggacgcaa tcccgtcatg
    19201 ggatctgttc ggagcacatc ctgatattta tccagagcgg ttttgtaatc tttttttgaa
    19261 taataggccc aggccatatt atacagagcc atggacggcg tatcatacaa atgattgccg
    19321 agggcttttt cgaattctct gatggctttg tcatagagct gtatttccga atatagcgtg
    19381 cccaggtagt tgtgagcttc ggaataatgc ttgtccaaag agaccgcctt ttcaaattcc
    19441 tcgatggcct gttctctcat gcctttgccg tgataggcaa ttcccagata ataatgcacc
    19501 ttgggatttc tggaatagta tttttcagct tccaggagtt ctttcagagc gctgttgaat
    19561 tgtccgatct gcaggaaaga aatgcctttg ttcaagaaga tctcggactg ttctctgtgc
    19621 cagggtgtat atgtacagga aaaacacagc aggattgctg agaacacaaa tattaataca
    19681 gctttgttcg cggttagttt cattttaata taaaccagtc tgttacaaat ccgatactgg
    19741 attgcagatc agagtcaaat aaaagccgat aatctgatgg ccggcagttg ggatttttca
    19801 ggttccttct gctgtaaagt cgttattcaa tatttttgga gtcacaaaaa tcagcaattg
    19861 ccgtttatct ctttttgtgc tgtccgtttt aaataaccag ccgagaacgg gaattttttg
    19921 cagccacggc cagccactta cggctttgct ttccgttgat ctcaaaatgc cgccgacgac
    19981 gacggtgtct ccatcgttaa taaccacctt ggactccacc tcgcttttga caattggagg
    20041 attgccctgg ggattcaggg ctgctttcga ccagtccgga gtatcattgc tggccttgat
    20101 atccatggag attttccctt cgtcggttaa cttcggcgtc acttccagtt ttaataatgc
    20161 ttccttaaag gaaacagttg ccggcgatgt tcccgatgcc ggagtgatat aaggcacttc
    20221 atcgccctgt ttaataatgg ctttgatacc ttccatggtc accaccttgg gcgccgaaat
    20281 gatttttccg gtggaattcg tttccagtgc ggcaagttgc gtttcaagaa aaccactggc
    20341 gcccccaaag atcagaccga ttgccggcga agccagggat gtgggcaaat tgactgcggc
    20401 catcgctaac ggattcccat cggcatccct gatgccgatt tgagaaggat atgtaagcgc
    20461 ctgctggttt ccggtggcaa cattgctgcc tccggatgcg cccaatccat actgactgct
    20521 gccgatgtgt tgtctctgta ccgtaccaaa tccccattgg acaccgaggt tcctgacgaa
    20581 ttcttcggtt gcctcgacaa tttttgcttc aatcaagacc tgcttcaata atcctttttc
    20641 ggccatcatt ttctgttctc tggcccggac ctcttctctg gcggtttctt ctcgcttgct
    20701 gcgttccatg ctggttacga taataacgtt gtcttcctgt ttttttgtca gaccgttaac
    20761 ttcaaggatg gtttccaggg cttttgtcca gggaacattt ttcatggaaa gcgtaatgtt
    20821 gcctttgact tcttcgctgg tcacgatgct tacattgccg tattcagcca tcaggcgtaa
    20881 aacgcttttg atgtccgcat cctgaaaatc aagagagacg attctatcca tatatttttt
    20941 cgccggagat ttttttaccg acacgtccag gtcttctttt ttaaaatcgt cggttttttc
    21001 cggttctgcg ctgactttgg ccaggggctt tttagaagca gaggcttttg atatcgttct
    21061 ggcctttctt tcctcaatgc cggaaacatt aaaatcaatt aaaacatttt ccccttcctg
    21121 tcttattgaa tagggaacgg cctcatgcgg ataaatcgtc agataaaccc agtttttccc
    21181 ctggagctgc tgccgcgaag gaacgatact gataatattg ttgagttccc cttctcccag
    21241 cgggcggcag agactttccg ggatatcggt atcttcaagt ctgatcaggt agcttccgtt
    21301 gtctttgctc ttaacatcaa tgagcggttg cttggaaaca accagatgaa tcctttcttt
    21361 tccgggcaac ttggccagca tgatactttt caacatggtg atgccggcag tgttttcttc
    21421 ggaagcattg aaagctgcaa aagaatttcc catgatacat gtgaagatcc atgtgataat
    21481 tacaaaagcc gcgaatattt tagggaaata ttttttcatg actttacctc attttaattt
    21541 ttgtgcagtt ttaaaattac tttgtttcct ttattatttt catattcttc tacaatgacc
    21601 ctgtcaggca gaatgtcaac gactttgccg ttatgcaatc cgattcttgt tccgaccagc
    21661 agaggataaa actttttaga agcgtcttct gcgatcgcta cccgatgatc ctcattgccg
    21721 acaatgccga cgactttata acttctggct tccgctcttt gcaaagggaa aatggacggt
    21781 ttgatttttt tctcttccgg cttcgttttt ttgacggttt caatgacaat aaaaggtcgg
    21841 aacggatccg gcttacccgc gggattgtaa ctgtatgtgt ccgcctgatt cggcacggtt
    21901 ggtgccggca taggggtttg gacagttggc gttgcagtcg catttgccgc ggaagaaacc
    21961 gttgtttgtt gagccgggga aacggccgga gcctgttttg ccggagccgg aacaggcgtt
    22021 tttgcggctg cgggcgaaag agccgtccgg gccggacctg gaacaaccgt tccggccggt
    22081 gccggttgga ccttgccttt aacgggggct acagactttg gagcaggcga ggccgtattt
    22141 tgggcctctg ccgcaaaagc aaaaaaagca gtggtcaaca ggatcaggct acaattaatg
    22201 aaaatcaaaa atattttttt cattgtgttt tttcacttgt cttttctttt ttatcaataa
    22261 acatgtatgt ttttatcgta caggatccgg taacaagatg gcctttgtct ttgatttctt
    22321 ttcggtctcc gattgtaatg tcggatacat taacgattct gggcagtttg gaaactttgt
    22381 cgaaaaaata aaggatattc tggtagcttc ctaaaaccga tattccgaca ggtatttcct
    22441 cgtagaaagg ttccgtagac ggagccgcgc catctttctt ttcagaagaa tccgccggtt
    22501 tttgatcgcg ttgatcagaa ggtttcagca aggctgcggt tttcgaagcc gcaggtccct
    22561 gggattccag ggatttgggc acggatgctt tcggttcaaa taatacaaat tccactccgg
    22621 cgtccctgcc cgctgacgcg acggagtgga aaagtgaagg tatctcccgc tggtcgggaa
    22681 gtttttgtaa cgctatctta taacttgcct gcaacgcttc aacatcggcc ttgtatttgt
    22741 taagctgcat agcgatcttt ttttgctcat tgattttatt ctgcatctcc gtaagttgcg
    22801 tatttaaagt ggttcttttt tccatggcgg aagacagaaa ataaaaccag taaaaataac
    22861 cgattaataa aaatccgaca atcacaatca atacttttgt cttaggggga attttcttga
    22921 tggtattgat gtcgatggcc atatttcaaa accctttttt cagaatacag gcgaaattaa
    22981 actgttggac agcattgccg gagatttccg tctttttgga agagaccaga tcaacggatt
    23041 gtaccggttc gaactgctca atgcttttca tgaaatttgc gacagcaatg ttatcccggc
    23101 ccatcccctc aatattgaga gaatttcctt tctgagtcag ccggacaagc cagatgtctt
    23161 tcggcggaac aagcatcgct aaattgtcca gtgttttaag aggcgtcagg cggttttctt
    23221 ccagagttgc aatgacgcct attttctgct ccagttcttt tttatctttt ttgaactttt
    23281 ccagatcgcc aattttctta ttcaaatcgg ttattaaagc ttcggaatct ttgatctgcg
    23341 tttccaggtt actgatcgaa gattttatcc agattgttac ccaggcgagc aaaagaataa
    23401 acaacacgac ggatccggcg acaataaata tttgtctcag gaacgtatct ttcttggcct
    23461 tctcacgata gggtaaaaga ttaatcttaa tcatttgtcg cctattttcc tcaagcccag
    23521 gccgatagcg gttgctgcaa tcggcttgat gctgtctaaa tttttgacat caatttttcg
    23581 tttgttgtaa ttaatcttta aaaaaggatc catgatttcg gttttaacat tgagcaattc
    23641 cgataaatgt ttggtgatgt tgggaattct ggacgagccc ccggcgagca acacctgctt
    23701 gatatcctcg ccgccaaacg ttgaacggaa atagtcaatg gacctttcaa tttccatgca
    23761 aatcggctgg gcaaattcca ggatgttatt tttcagatcc aggttgtcgt tttcgcttcc
    23821 ctcgggaaat ccttcgattt taagcttttc cgcttcttca aaggggatct gatatttttc
    23881 ctggatactt tcagtgatgg aattgcctgc cagcgcgaaa tctcttgtga acaccgacat
    23941 tcccccttta accacattaa tgccggtggt gcttgctcct atattgatca gaacaacgat
    24001 ttcctcgttg tcaaattcat aattgacttc atataatgtt tccaggacaa acggagccac
    24061 gtcaacgatt tcaacattct ggccggctgc tgttactgct tccagataac tgcttacaat
    24121 ttcttttttc gctgcaacaa tgacaatatc catctgattc ggattgtaat tgttttcgcc
    24181 gagaatttga aaatcataat tgacatcgtc catgttatca aaaggcagat atttcccggc
    24241 ttcatcgtgg atcagttcac gcaattccgt ttcgttcatc tgctccagcg tcactttctt
    24301 gatgatgacg gaatttcccg ccagagacgt gacaacgcct ttacggccgc aatgggcaat
    24361 cttgaaaagc tccttgattt ttgaggatag cacgggtgca tcggcgagaa tgccgtcaat
    24421 aatgatgcct ttgggcaggg gaacttcatg gaaacggttc agatagtatc ctttggcgtt
    24481 gctgatgatt tccgcaagtt ttacggagct tgaaccaata tccaaaccga cgagactctt
    24541 ttttccggaa aatatttctt tcaaatccac catcaaagca ccataaattg gttatcggga
    24601 agtcatcttt actttgccca ccggtaaaca atatctcctt ttttaaccat tcccaattcg
    24661 ttacgggcta tcatttctat gtaattaatg tcgttgcgca ataaaataat tttctttttt
    24721 aatgcgttgt tttctgctgt tatggcgctg ttttgcgatt ttaattccgc cagccttttg
    24781 ctcatcaagt aattgtcaac gagacccctg ttgccgaatg tgatcaaaag cgacattaac
    24841 agcaaaaata taattagcaa tctgccgaca atcttcatcg aaattcaaaa ctactccggt
    24901 acggaaatga ttgaatatat tattcgcctt catcaaggga atacttcttc atgtggcctt
    24961 tgcagtgctt tagcgacctt gagttcagaa ttttttctgg tggacaatcc tttttccatt
    25021 ttggaaattg tttgaggtgt taatccggcc cggcgagcca gttcggatat gcttaaagga
    25081 attgtctcac gaaatttttt tacgtgattt ttcttcatat tatctgccat agattaaagc
    25141 gacacctcac ttgcttctca taaggaacta attttaaata aatgtcaaga atttttttta
    25201 atattcatta atattgctta atattcatta atattgctta atatcgatta attttgaaaa
    25261 agaacaatgg cgagactgaa cacgctaatt caatgaataa aaaattgtta attctattgc
    25321 ggaaaagaaa tgatttataa aaaaagcgca acggtctggg accggatgtt tttgagcatg
    25381 ttaacggcaa accccttgct tttcggctat tttatgtaat ttgcaatttc ccagttcgca
    25441 ggcctttttt gcgtcgagac aaccgtattg atggtttccc agtgacagat aatcagcccc
    25501 ccggttgttg taggccctgg cgttgtcggg tttcagcgct atggctttgc tgtattccgt
    25561 aatggcctgc tgatgttgac caagttgatc ataagcaaaa ccaaggccga tgtaattgtc
    25621 ggcctctccg ggattgatgc ggatggattc gttgaaatcc gcaatagcca gctgatattg
    25681 accggtcgtg ttgtataagg tccccctgtt atgaaaagcc tcggcgtaat caggtttgag
    25741 gcggacagtc tgactgtaat ctttgatggc ctgctgatgc cggccaaggt tgtcgtatgc
    25801 aattcccctg ttatagtatg cctcggcatc attcggctga agtctgatgg cgttattcaa
    25861 atactcgatg gctttttgcg gatcactgaa tttttcacca tccgataaag cttgagcctt
    25921 gttgaaccag tccgacgtcg tttcgctcat tgccggggat ccgagcaaag taaagataca
    25981 tagaagtgta atcatcatct tatacataaa aaggtcttct tccgatttgc atttcaacgc
    26041 ggatcattta ttccacccag gccgtgagat tttccgccgg ttcgcgctcc gagcggacgt
    26101 gattggcagg gaatgtttcg tccggatagc cgacagcaat ggcaatcatc aggcgcttgt
    26161 ttgaagagat gccggtgcag tggcggacca cttcaggata catgaccccc tgatcttcaa
    26221 tacaggtgcc gactccgaag tgcagcgccg tcagacagat gttttgcatg accgcgccga
    26281 ggtccatcag ggaagcatga tcgcctaata ttttgtcgct gacaatgatg atggccgccg
    26341 gagcatcaaa aaaacgaaat ccccgttcca tccaccaggc gcgtttttcc ttgtcttcac
    26401 gggcaatgtc catggctttg aagagttgct tggccagcgt gatctggcgg tccttataaa
    26461 cgcttttatc aggccagccc accacctgat gatccggtgt aatcggcgca ccggacgtca
    26521 gcttgtcaac gatggcggca cgcagtttgt tgaggggctc gccgctgacg acaatgaatt
    26581 cccagggctg cgtattcatc gccgacggcg cacggcaggc gatattgaga atttccatga
    26641 tggtttcttt gggcaccggc tgatccgtaa acgcccggat gcttctgcga tgctgaatgg
    26701 cttctatcaa atccattgta aacttttcct tttcctgtgt tgtccggaat gcctcatcgg
    26761 ttgcaaaaac aacctcgccg ttgaggtcag attgtttaaa tcgtttacgt ctgaatgcag
    26821 cgttgataac gttgtcacaa atttcgatga tgataatatt ggcggaagtg catgggaatc
    26881 gaacccacct gggacggttt tagcgcccca caccggattt gaagtccggg aggcccacca
    26941 gtgaccttgg cacttccgcg gaagaatcta tccggaaaga agaaaagtgt caatcatcaa
    27001 aagtttatca gcagaatttt catctcggat cgtattttca taattgcaga gcatccgacc
    27061 aggataacga atcatcaagc aactgaactg tgacctggga attttttttg attcgggttt
    27121 cgttttctcc catgacaatg aggccgttgg ccgtgaccat cgatttcaaa atgccggagc
    27181 cctgatcgcc ggttgtcacg gcaatatatt tgtctttctc cttccggacg acggcacgga
    27241 tgaaatgtct aaagccggca gctttttcaa attcttcgtg gcagattgcc gtgacggtct
    27301 gacggaagat ttttttatgg ccctgcattt tcaaaagcga agggcgcaca aattgctcaa
    27361 aggaaaccat tgccgatacg ggattgccgg gcaggccgaa cagcggaacg ccttctatgg
    27421 caccgaacgc caggggtttc cccggtctca tggcgacctg ccagaaatgc atggcattgc
    27481 cgatttctcc catgacattt tttacgaaat cgaaatcgcc cacggacacg ccgccggatg
    27541 agataatgac atcggcgttc agcgcttttt tgaatttttt ttccaaatcg gattttttat
    27601 ctctggcaat ggcgaggatc aacgggatcg cgccgcactc cgctacttgc gctgccagag
    27661 aataactgtt gctgttaacg attttgccgg gcggcgggtt ggtcctgatg ctgaccagtt
    27721 catcgcctgt tgacatgatt gccacgcgcg gtttttgata aaccttgagc gaggattttc
    27781 ccagcgcggc aagcatcccg atatgcgcgg gacgcagcaa gctgtttttt gggatgaccg
    27841 attcgccttt acggacatct tcaccggcga agcggatgtt ttcccccctc gcggctgaag
    27901 cgaaaatgat gacggttctg ccgtctttgc gggtgtcttc ctgacggata accgcgtccg
    27961 cccccggggg aatcacggcg ccggtcatga tgcgggccgt ttcgtttttg ccgatgttct
    28021 ttcggggaaa tttccctgcg ggaatgtctt cgataatttt caattttaca ggagttgtct
    28081 tgcccgcgcc cctggtatcg gcgcaccgca cggcatagcc gtccatggcg gaattatcgg
    28141 cagaggggat attacgcggg gacataatat tttcgccgat gacgcgtccc ctcgctttca
    28201 gaagatggac tgtttccagg cccagcggct ggaaattaac cagaattgtt tttaaggctt
    28261 tttctacagg aatcatattc aatctccgcg gaattgatgc gttccagttt atcaaagatg
    28321 ccgcgcttgt caatataaag atagattttt aatggcaatt ttcggaaaac aagttttcaa
    28381 ttgaattatc cgggaaagaa gaatagaaca cgtgcaaaca tacccgttcc ggatcaaaag
    28441 aactttttgg atgcggaatg ggttttggtg ttgtttgatt ttttggggga ttgattgccg
    28501 tgcggaaaat gatcacaagg aaaaacccgg aagatatgga gtcgtttcgt cttctgctga
    28561 aacagatcga tgacgggatg atgcgcatcg ccgtacaggg ccttgccggc gcggcccgtt
    28621 cttttctgat ctccttattg tataaaaaac gacagaggcc tgtactggtg gtctgccccg
    28681 aggagaagga agcggtggcc tttgctcgcg atttgtcgct gtttctgggt gaagagcacg
    28741 tgtttcatta tccggcgctg gatttcctga ccattgacat gtttgctctt cagcgtgacg
    28801 aggaattgtc ccgtctggag gtgttggcca acttgcagat tcaacacaaa attattgtcg
    28861 ttgcctctgc aaatgcgttg atgcagaaag tgatgccggt ggccgagttc agcaagcatc
    28921 tgcgaattat tgctcccggc gacaccatca accgggatga atttgcagcg cagcttgtcg
    28981 cgcaatgcta taaacgagaa agtctggttc aggaaaaagg ggaattcagc atccgcggaa
    29041 acatcattga catttttcct cccaccgaaa aaaatccgct gcgcctggag atgctgggcg
    29101 atgaaataga gtccatcagg acattcaaca catcttccca gcgttctatc ggaccgttgc
    29161 ccgcttttgt attatctcct gccggagagg ccgtcatgga tcattcgacg ctggagctgg
    29221 cagtgcgcaa catccggcgc cgtgccgacg aactttcctt ttcccgcgac attcgcagcc
    29281 gtctggttgc cgccgttcag gaggggttga ccaatgcctt caatccgata ttcttgccgc
    29341 tgttttatga atcgtacgat accggaagcg gcctttccga taataccttg tccggccttt
    29401 tggaatatct ttcaagaaat acactggtcg ttctggatga tccgctggct gttgggcacg
    29461 cgatgttcaa cgctgggttg gctatggata aattgctgtt caaggcaaag agcggtggaa
    29521 aattctatct ggaaaaggaa aatacctatt tcccgccgga ggctgtaacg gccggcctgg
    29581 atggcttcct acaggttgcg ctggctggac tcgaaatcga atctgaaaga tatcatgtgc
    29641 cggtagtttt tgaaagcgac cggttcacct gcacagggga gccgctggca ggtgaaatcc
    29701 gagaggatgc aacgctacgg ttgaccgtag aaaaaataaa atcatggttt gccggggaca
    29761 tgctggtttt tcttttctgt ccgttgccgg aagatgttca acggatgaag gggttgcttt
    29821 cgtcttacga tctgccggcg gagacattgt ccggcgctga accggttctg gacagcctgg
    29881 aaggttggaa aggcgatgca cggttgattt tattcgtggg agacatttca tcaggctttg
    29941 tcatgccggc gataaagctg gccgtcttaa gcgaagaaga aatcttcgtc aaaaaagccc
    30001 gccgtctgcg ctcgcggcca gtacgcacgg ggtattttat caaatcgttc ggcgatctgc
    30061 aagaaggcga ttttgtcgtg catacggatt tcggcatcgg catttaccgc ggtttgaaaa
    30121 aaatcgccgt gagaaagatt gaaaacgatt ttctggtcat tgaatattcg gagggcgata
    30181 aattatacat tcccgttaat gctctggaga agattcaacg ctatgtcggt cccgacggct
    30241 atgtgccgaa gatcgaccgg atgggcggaa catcctggga ggctgtcaaa gaaagggtga
    30301 aaaaatcggt ccgcgactat gccgaagaac tggtgtccat ttatgccgcc cgcgagacgc
    30361 tggaacgaaa atcgtttgcc ccgccggaca gaatctacga agagttttgc tcgacctttg
    30421 aatttgaaga gacgccggat caggtgaaag ccatcgaaga cattcatcag gatatggatg
    30481 acgtcaagcc gatggaccgc ctgatctgcg gcgatgccgg attcggcaaa accgaagtgg
    30541 cgatccgctc ggcgtttcgc gcggtgatgg acggtaaaca agttgctgtt ctgaccccga
    30601 cgacgattct tgcggaacag cactatcaga cgtttacccg ccgttttgaa gattttccgg
    30661 tgcgggtggg actgctgaat cgttttaaaa aacccgccga acagaaaaaa attgtcgaag
    30721 agcttaaaaa tcagacaatc gatattgtcg tcgggacgca tcgtcttttg caaaaggacg
    30781 ttgcgtttca agatctcggg ctggtcatta tcgatgagga gcagcgtttc ggcgtggcgc
    30841 ataaggaaaa gttgaaaaaa atgcgcacgc tggtcgatgt gttgacgctc tcggcgacgc
    30901 cgattcccag gacgctgcat ctgtcgctgg tcggcatccg ggatttgtcc gtcatcaaca
    30961 cgccgcccaa ggaccgggtt tccatcaaaa cctatgtcat ggagttcgat gaggatgcga
    31021 ttaaaacggc ggttgaaaaa gaactggcgc gtcaggggca ggtgtttttt gtccacgacc
    31081 gggtgcgttc gatttatgcg attgcccgcc tggtgcagcg gctcgtgccg cacgccaaag
    31141 tcggagttgt tcacgggcag atgaagccgg cggaaattga aaaaaccatg gccgggttca
    31201 tccggcaaga gaacaatgtg ctggtttgta caacgatcat cggttccggt ctcgatattt
    31261 cgacggccaa tacgattatt atcaaccgcg ctgaaaagtt cggcctggcg cagctctatc
    31321 agatacgcgg ccgcgtggga cgatccagcc gggaggcgtt tgcctatctg ttgctgcctc
    31381 agggagcgat actttcccgc gcagcgatga aacggctgca ggtcattaag gaattttcgg
    31441 atccgggctc cggtttccgg attgcataca acgatctgga aatccggggc ggcggaagcg
    31501 tactggggat gtcccagtcg ggacatattt ccgccgttgg ttacgagctt tatactgaat
    31561 taatcgaaaa aaccattcgc gaagtcaagg gggagaaaac cgaagaagac tggatgccgg
    31621 aaattcagct tgggatttcc gcattcatcc ccgaggatta tgtgccggat gtccatcagc
    31681 gtctgatttt gtataaaaga atatccctgg cggcaaacga tgaagacatc cgcgcgattg
    31741 aaaatgagtt gctggattgt tacggcgaga tgccccgtgt tgcggataat ttattgcggg
    31801 tgatctccat ccgaaatgtt ctgaagccgc tgaaagggaa gaagatgggc tatgacggta
    31861 aatacctata catcttcttc cgggagaatt ctccggtcga tccggggaaa ataatcgctc
    31921 tttaccgcaa gaaaatcaaa gaattgcgtt ttaccccgga ccataaactg tttgtgccgg
    31981 cgccggctgc tgcggatatg gacattctgg ttcaggcgga tatgttgtta aagatgcttg
    32041 cccaatagcg gtaagcagga tatacagaaa gatgatgatt ccaaccagat ggttttctaa
    32101 attttattct ttggcgggaa aatccaaaaa ggtttctccg gttcgctgcg gtcttttgtg
    32161 tctgctcttg ccggggttaa tgatcgcctg cgataaaatg gaaagctttt cgcgtcccca
    32221 tgtcgccacg gtcaacggtg ccaaaattta tcttgatgaa taccagtccc ttttgaatga
    32281 gaaggccagg atgctttccg gcgatttctt tagacagccc gcgtatgtga aaaaattcga
    32341 ggacgaagtc ctggatgcca tgatcgggga aaaaataatg ctcttgcgtg cccgggaatt
    32401 gaacctgtcg gtgagcgatg ccgaactgga ggcaaaaatt aaagaaatcg aaaaagatta
    32461 cggaaacgaa ttcaccggtc tttttgccag acaaaatatc aactatgaga aatggaaaga
    32521 ggattttcga aaggaaattc ttctgcagaa acttattgcg gcggatgttc attctaaaat
    32581 tatagtttcc gaaacggaaa tagaagatta tttcaaaaag caccgtcaca tctacaaaag
    32641 ggatttgtct gccagagtgt cgcagattgt ggttcgggat atgcaaaccg ccgagcaggc
    32701 gatgcaacga ttgaaatcca aggaagaatt tgcccgggtg gcttcggaga tgtctatcgg
    32761 tccggaagcc tcgcgtggtg gtgacctggg attcatcacg aaacgggtca tgccggaacc
    32821 gctggatgaa acgattttta aaataccggt ccagacgatc agtccgatcg tacagagttc
    32881 ttatggattt catattatca aggtgatgga agtccggcag gccaaagaaa gaatcttgcc
    32941 ggaggccaga gaagaagtga ttgcggatat tcgcctgctg aaggagaaaa cggcttttgc
    33001 ggaatggctg gaagccttaa agaaaaaagc cgttgtccaa aaaaaagccg atattaaaat
    33061 caatacatca tacaggtagc acgatcaagg gaggtctgtg gtgaaatttt acggaaaata
    33121 tgtttttgca ttactgggaa tcatgctttt gtgtccgtta ttgcacgccg aagttgtgga
    33181 taaaatcatt gccgtggtca atgatgaggt gatcacccgg cttgagttga atgcagcctt
    33241 cgaaccgtat cggaaaaata ttgagaatac ctaccagggt ggcgacaagg accaatttgt
    33301 tcttcatgcg agggaggaat ttctgaaccg gctgatcgac aatatcctga ttgagcagga
    33361 ggcgaagaaa acggggatca gtgcttccgt ccgggaagaa gacgttatgg gtgttcttca
    33421 ggacatgctg accaaacaga atatcagtat gcaggatttt ttgaaaaacc ttgccaagga
    33481 cggcagctcc attgaggagg tcaaaaaaga aatacggtca caaatgatcc gtatgcgatt
    33541 attaagacgg gaagttaaag ataagattat cgttaccgat gaggaaatag gcgcctatta
    33601 caatcagcac cgcaatgatt acgagggtaa agaaagcgtg agaatgaaac agcttttatt
    33661 gctgctgcct tctggtgcca acgcggagac aaaaggaaaa atccggaaag aagctttgca
    33721 cctgagggaa cgtatcatgg cgggagaatc ttttgaccta ctggtttcaa cgtattctca
    33781 ggggccggcg gccgctcagg gcggggatgt cgggtttatt gagaagggga ccatcatccc
    33841 ggaagttgag gcggttgcgt tcagccttgc cgtggatcag gtcagtaacg tgattgaatc
    33901 cgatgcgggt tttcatatca taaaggtcgt ggataaacgc ggcgccggat taaaaccggt
    33961 tgccgtggtc cgggaggaga taaaagcaaa aattgaagat gaaaaactgg ataaaaaatt
    34021 tgaggaatgg atcagtgcca ttcgcgcaaa atcgcatata gagatcaaga attaaagcaa
    34081 agaataacat ggacgcgatc aaaatcaggg gcgccgctca acataattta aaaaatataa
    34141 gcttggatat tccccgggat caatttgtca tcattaccgg tgtgagcggt tcaggcaaat
    34201 catcgctggc tttcgacacc atctatgctg aagggcagag gcggtatgtg gagtcacttt
    34261 ccgcttatgc ccgtcagttt atcggacaga tggacaaacc gaatgtggaa tccatcgacg
    34321 gcttgtctcc cgccattgcc atcgagcagc gtacggccag ccataatccc cgttccacgg
    34381 tggggacggt gacggaaatt tacgattatt tccgcttgct ttatgctgcc attggcgtct
    34441 gccattgtta ccggtgcggc cgggaaataa aatcacagac catcgacggc attttggaaa
    34501 atgttcttgc cttgccggaa aacacgccgt tcagtgttct ggcgccgatg gtgagaggga
    34561 aaaaaggcga atttcagaag gaattgaaaa aactccagaa ggaggggttt gcccgtgtcc
    34621 ggatcgacgg agaaaacagg gacctggcgg aagacatcag cctggaaaag aataaacgcc
    34681 acgatattga ccttgtgatt gaccggttgg tgctgaaaaa cggtatcaga aaaagattgc
    34741 gggattctct ggagatcgcg tcgaagcatg ccgaggggct ggtgcggatc gtgaccgccg
    34801 gcggcgaaga atttcttttc agcgaaaaat atgcctgccc cgattgcggg atcagcttgc
    34861 ccgctctgac accgcgcatg ttttcattta ataatcccta cggagcctgt cccgcctgca
    34921 acggtctggg ttcttccatg cgttttacgg aatatctgat tgttcccgat gccggattgt
    34981 ctctgcggga cggcgcaata gcgccgtggg ccggtcgtca ctcgctttac tattacagca
    35041 ttctggacgc gctcagcagc cattacggtt ttgatgtgaa cacgccgttc aataagctgc
    35101 cggaaaaaat tcggaaagtt cttctttacg ggtcgggcag cgaaacaatc aaattctatc
    35161 atgatcgcgg caataaaaga tattttgttc accggccgtt tgagggggca atccggcaat
    35221 tggagcggcg gtggcgggaa accgcgtcaa cggcgattcg caatgatctg atgcgttata
    35281 tcgatttgag cgcctgcgag acctgccacg gggcgcgttt gaaagaagag attctggccg
    35341 ttacggtgat gggaaaaaat atctatgaat tgtgcaaaat gtccatccgc gattgccaca
    35401 gctttttcga acggatcgaa ctctcaccac aggaactcaa tattaccgaa agaatcatcc
    35461 gggaaatcaa aagccggttg cagtttctgc tcaatgtcgg aatggattat ttgaacctgg
    35521 agcgagccac ctcaacatta tccggcgggg agagccagcg cattcggctg gccacgcaga
    35581 tcggttccgg gctgacaggc gttttgtatg ttctggatga acctacggta gggctgcatc
    35641 agaaagataa tctgcgtttg atcgatacgc ttaagaaact gcgcgagatg ggcaacacgg
    35701 tgctggttgt cgagcacgat gccgacatga tcatggcctg cgaccatatc gtagatatcg
    35761 gaccgggggc cggaacggag gggggagaaa tcatttttca gggcacgccg gtacagattc
    35821 tgaacagtga aacgtctctg accggcaggt atttgtcgaa aaaagaatcg atcaaccttc
    35881 cctcagaaaa acgtccgctt accggaaggc acattattct tgaaggcgcc agcgaaaaca
    35941 acctgaaaaa tatcgatatc aaaataccgg tgggtgtgtt tacggcggta accggtgttt
    36001 cgggatccgg gaagagtacc atggtgattg aaacactcta caaagtgctg gcgcgccgtt
    36061 tgaatcagta caacggatcc atggggaaaa tcaaaagggt tgtggatctt ggcgggattg
    36121 aacgcgtcat tatgatcaat caacacccca ttggccgcac gccgcgctcc aatccggcaa
    36181 cctacacggg cgtcttttcg tttatccgcg atctgttcac caatcttccg gagtccagaa
    36241 tgcgcggata caaaccgggg cgcttcagct ttaacgtgaa gggcggacgc tgcgaggcgt
    36301 gcgaaggaaa cggtttaatc aaaatcgaga tgcattttct gccggatgtt tacgtgacct
    36361 gcgaagcctg tcatgggaaa agatttaatc gcgacaccct tgatattaaa tacaaggata
    36421 aaagcatagc cgatgttctg gacatgacgg tcaatcaggc gttgaccttt ttttccaata
    36481 ttccttccat ccggtcgcat ctgcagttgc tcggcgatgt cgggctggga tatattcatc
    36541 tcggacagtc ggcgacgaca ctatccggag gagaggcgca gcggattaaa ttggctcggg
    36601 aactgagcaa gaaagtgaac agcaataccc tgtatatcct ggacgaaccg accattggtc
    36661 tgcattttgc cgatatccag aaactgctgg atgttttgat gcgcctggtg aatatgggca
    36721 ataccatggt tgtgatcgag cataacctgg acgttatcaa gtgtgcggat tacgtgatcg
    36781 atctcgggcc ggaaggcggc tccggcggcg gcagaattat ttcgtcggga acggtcgaga
    36841 aagtagcgcg agatgaaaaa tccgtaacgg gcaaattttt gagagatgtg atcacggcct
    36901 gaaaatatgt cctttttgat cctgtcattt ttccctgctt gcagaaacat tgagtgcttc
    36961 ctcaggtcat tcgttgtctg aaagtaaaaa tatcttgaca tcctcatgag aatggttttt
    37021 tattttataa aattcttcag cggtgtcatc aggaaaggat attaacctgt ctaacgattc
    37081 cggatatgag cattgcatct tgattgttga tgacgaagat tcaattctca atgcttttaa
    37141 gcgcattctg gccgacgaaa attacgacgt gcatgtcgca aacagcggtt tggagggatt
    37201 gaacaaactg cgttcggcca caaaacccta ttctattatt atttccgatc aaagaatgcc
    37261 tgaaatgagc ggtgttcagt ttttctcgaa ggcaaaagaa atttttccgg atgccatccg
    37321 tattcttctg accgggtatg cggactccga cgccattatt gaagccatca ataaaggcgg
    37381 cgtacatctt tacttcacca agccctggca tgaagaagaa attctgctgc atattaaaca
    37441 atcgctttcc aaagttgaga ttttagcgga aaataagcgt ctggtggaat tgataagaga
    37501 gaaaaataaa gagctgacgg agttaaacaa ggctctggag caaaaggctc tggagaaaac
    37561 caatgacctt ttgattcaaa cggaaaaact caaggaaagt tatcaaaaat cgcagatcat
    37621 tatggacggc atcgtcagaa cgctgtccaa aattatagag acgcgggatc cttatacggc
    37681 cggccacgaa gaccaggtgg ccaaaattgc ctgcaagatt gcccgggaga tgaaattacc
    37741 cgaggagcag atttcgttta ttcatattgc cgccaccctc catgatatcg ggaaaatatg
    37801 cgttccgtcg gaaatattaa caaagcccag tgcattaaac aatctggaaa aggccatcat
    37861 caaaacgcat tgcaaagtgg ccaacgatgt gctggcgaac atagaatttc cctatccggt
    37921 ggcggaggtc atctatcagc atcatgaacg attggacgga tcggggtatc cgagggggtt
    37981 gaggggaaac gagatcgcgc tggaagcccg cattatcggc gtggcggatg tcattgatgc
    38041 catggcttcc tacaggccgt accgtccggc tctgggcgtg gatgcggcta tcgaggaaat
    38101 cgtcaaatac cgggggatca cgtatgatcc cgatgtcgtg gatgcctgtc tgacggtcta
    38161 taaaaaaacc gcccgaacgt tgatcgagac caaccaccgc accgtgaatt gatggccgtc
    38221 ttttcagcgc ctgggatttt tactcaattc ctttctccga aaattgctct gccgatgcgc
    38281 accagagttg cgccttcttc aacggctaca tcaaaatcat ccgtcattcc catggaaagc
    38341 tcttccatgt gtatgccggc gattcccgcc cggatgatat tctgttgaag atttttaagt
    38401 tcgcgaaaat aaggacgcga tgtttcggga tcgtccgaat acggcgccat ggtcatcaat
    38461 ccttttaccg cgatatgctg catggcggaa agatcctgaa gcagcgcaac cgcctttcca
    38521 gccggaatac cgttttttgt tttttcaccg ctgacgttga tttcaatcag aatgttgatt
    38581 tgacgacccg cccgcccggc tcttttatcg atttccgcag ccaggtccag acggtcgacg
    38641 gaatgaacgt agtcgaaaag aatcaccgca tatttcgctt tgttcgtctg cagatgcccg
    38701 atcatgtgcc acggaatgct tctgccgatg acggcgattt tttctctggc ttcctgaacg
    38761 taattttctc ccagggcggt tatgcctgcc cggatcgctt cttcgatgac cggaacgggg
    38821 accgttttgg taaccgccag cagtttgacg gaatcaggag accgtttgcc tctttcggcg
    38881 gcggcagcga ttctctgttt gacagcggcg atattggcgg caatgtctgt tcccatatgg
    38941 ctaacaaaaa tcgatgacgt gcagtttttt cagcgtttcc aaaacttcgc agagcggcag
    39001 tccgatcaca ttggtgtagg aaccgcgaat ggagcggaca aaacaggcgc cttggccttg
    39061 aagggcatag cctccggctt tgtcgtaagg ttcgtcaccg gcgatgtacc actccatttc
    39121 gtccggatga atggttttga agcgtacaac ggactgaacc accccggtcg agcgaattcc
    39181 agaagacaac cggacaacgg taaatcctgt aaagaccgtg tgctgtctgc cgcttaattt
    39241 tttcagcatc gttttcgcct gtgttttatt gcggggcttg cccagaaccg tgccgtcgat
    39301 gacaacaatg gtatcggctc ccagaacaca ggcgtcgggg ttctcgacag ccaccttcat
    39361 cgctttcagg ctggaaagcc tgcgcacgtg ttggcgaggc gtttccccgg tgagatattt
    39421 ttcattgaca tgggccggaa tgattttgac atgcacaccg gcggaccgga gcatttcctg
    39481 ccgtcgcggt gacgccgacg ctaaaatcag tgacgcatgg gctgtgatgg acattcattc
    39541 cccataatag attttttttc ttgtatcaca aatttaaccg ttgctgtcaa ttgcaattat
    39601 gattgcaatc cgccgggtat tggagttatg atgttcgcaa ataaaaccgg tgttttgggg
    39661 aatgaagaaa aaaatcatta tatttgccgt tctgatgctt ctgggcgttt ttgtcagcgc
    39721 ggccgacgcg ggcgaggtcg tgatgcagtt gaccgtgcct ggatgtgccg cctgaggggc
    39781 caagcaaagg ataggggcta tcctgaacgg aattcaaggc gtcaaacact atcaattcga
    39841 ggaagggaat gtcgtgtcca ttacctttga cgatcagaga acgtctgtgg atatcattac
    39901 cggagcactc gaaagaaaag gattccccct caagggccgt ccggtatact ggaaacgccg
    39961 ttaaaggtat tttttcagtt ggcctgcttc cagtctttca acaatctttt taacatcctg
    40021 agaacgtcct tttttacaga tcagcagggc atccggcgtt tcgacgatca tcagatccct
    40081 gactcccagt aaggcggtca attttcgcgg attatagatg agcgatcctt tcgaatcttc
    40141 gagaacggtt ctgctcccgc tgaggatgac attattctgc ttgtcttttg gggagatgtc
    40201 ccataaagca tcccaactgc cgacgtcgct ccagccgaaa tttcccggca ggataaaaac
    40261 gttaccggct ttttccatca cgccgtaatc gatggagacg gagcccagtt ttttgtaaac
    40321 gcgggacacg gttgccgtct cgccggcggt gcccagcgat gtgtcgataa gtgtcagtcc
    40381 cgcatataac tctggcagcc agcgtttgat ctcttccaga atgaccgaag tctgccagac
    40441 aaacatcccg ctgttccagt agaaattgcc gctccggacg aattttgccg ccgtgcgaaa
    40501 atcgggtttt tcacggattg acttaacgcg gaaaacgtct tggtttttta ttgttttgca
    40561 taagattccc ccttcgatat aaccaaaccc tgtctgaaca ctggaggggg taatgcctat
    40621 ggtcaccaat cctttctcct gggccgccac aacggccgcc gctttcatca cgctcagata
    40681 ctttccggaa tcggctatgg cgtgatcgga aggcagaacc atcattgtat cgtccgtcga
    40741 tcgcttacgg atatgcaagg cggccagtcc gatgcaggcg gcggtatttc ggcctaccgg
    40801 ttcgatcatg atgtttcggg ggggaatttc cggcagttgt ttcattaacg cccgggcatg
    40861 ttttttaccc gtcactacga gaatatttgt cgggggaacc aacggcttta tccggttaac
    40921 ggtttcctga ataatggttt tttcgcctgt gatgtccaga aggtgtttgg gtctcttttc
    40981 tctgctgcgg ggccagaatc tgacgcctct gcctccggcc atgatcactg cgtacatgat
    41041 ttttctccct gtttttttgg cattaaaaat aaaaatgatg aatgcttata atataatgtg
    41101 tcttgcaatt tcaaggcgga aaatatattt tatgccacca tgatcacgat caactatccg
    41161 gaaatcattc taaagaaagc tcgagaagat gccattctgc gcggacatcc ctggatattt
    41221 tccggcgcga taggctcggt aaaaggaaat cccggccccg gcgatattgt tctggccaga
    41281 gatgcggcag gccatccgct ggccctgggc ttttttaatc cgtcgacgga tatcgctttt
    41341 cgtgttttga ctagaagatg cgacgaaaat atttcaccgt atttctggca gtcccgtttg
    41401 catgacgcat ataaattgcg gcagagactt atcaatgagc agacaaatgc ctaccgtctg
    41461 attaatgccg aaggggacgg atttcccgga ttgattgttg acgtctacaa tacgactctg
    41521 gttttttcga tcgccgtcgc cggaatggag aaacagaaaa atcatctttt aaatgctctg
    41581 atcgcccagc tcaaaccaac aagaatttat gaacaaagtg acagccattc acgcaagctg
    41641 gagggattgg aaaacagaag cggcatcgct tttggtgaag atgaaaattc taccgtggaa
    41701 attatggaga atggtctgaa attcgaagtt gatgttgttt ccggtcagaa aaccggattt
    41761 ttcctcgatc agcgggtgaa ccgggaaaaa atcggttccc tgggccgtga tgctcatgtg
    41821 ctgaattgtt ttgcctatac cgcggctttt tccgtttatt gtgcggaagg cggcgccaaa
    41881 aaggttgttt ctcttgatat atccaaaacg gcgtgcgttg ccgccagaaa aaacctgcat
    41941 ctcaactggt tttctgtgga gaattatccg gtgatcgaga ccgatgtgtt tgattatttc
    42001 agacatacgc cggaaaacta caacatgatt attcttgatc cgcccgcttt tgccaaaacc
    42061 aaaagagatg tgcccaaagc ggcaagaggg tataaagaca ttaatttaca ggcaatcaaa
    42121 catctgagca aaggcgggat gctggccact ttttcctgtt ccaatttcat tgaagaagat
    42181 cttttttata agatcgttct gggtgccgcg cgggatgccg gtgccgagtt gcagctttta
    42241 tcaaggctgg acgccggacc ggatcatccg gtcgctttcg gacaccccga aggacgttat
    42301 ttgaaaggtt tgctgctggt caaaagaaat tgacaatatt ttatctgcca tcggaatcag
    42361 ttcttcgcca gattgccgat aattctgtca tggatcagac gggaagccat ggtattgccg
    42421 aatgctccga caaagactga aacggaggtc atatctctgc cgacatactc aactttgatg
    42481 gtgacttttt cgtcctgaat gacggatgta atgacgccgg tggaaatttt acgttctttt
    42541 cggatacttt tcgctttcag atcggtcacg gattgttcac aggcctgcca gaccgcatcg
    42601 atatcggctt tgtaatcgct gaccagatgt ccatcctggt agaaaaacct gccggaactt
    42661 atgccggcga ccttcccctg taaattcacc gccgcaccgc aacctgcaag taataagcac
    42721 attataaaga agaaaacggt tatcgtattt ttcatgatat caccttccgg agcgcttcaa
    42781 ataatctgtt gacgatcaat ggccgcggca tacctgtata aggggcgtta cctttgagcg
    42841 gctttccttt cgcgcaggtt catgatctct gtccgagtca gaatggaagc gatgttgtcc
    42901 atgtgcttca tgatattttc ccggccccct tcttcccggt cgacaagcgt tatcaccatg
    42961 acaacggaca gtccggcttt gcgggcctgt tcaatggccg tgattgtgga tccgccggtc
    43021 gtgatcacat cgtcgataat ggcaactttt tctcccggcc ggacactgcc ttcaatggcg
    43081 cttttagtac cgtgatcctt ggcgtccttt ctcacgataa acgatttgat cggtttgcct
    43141 ttctggtagg agataacggc aagcgcattg gcgatgggat cggctcccag ggtcagccct
    43201 cctgcggccg taatatcggt atctttgagc atgtcaaaaa tgatggtgcc gatcagattc
    43261 atcccctcgg ggtccagggt cgtcggttta cagttaaaat aataattgct ttgcctgccg
    43321 gaggcgagcg taaaaggggg attgtcgctg tatttgaaag acctttcgag aatgatttct
    43381 cccagtcgtt ccttcatttg atcaatggtc attgtggttc ctccgttcgg atgaaattta
    43441 tgattgaggg tgatgttgta acataaactt gatcggcagt tcaacccagg cggtgaatgg
    43501 atttccagct ttcttcgccg gttcgaattt ccagggcttg acggcctgta ttgcagattg
    43561 atccagaata gcgtatccgg atgatttccg gatcttgacg tcgcccacgc gtccggtcgg
    43621 cagaatttcg gcgaagacca gaacgatgcc ctcatatccg cgcaaacgtg ccatttccgg
    43681 ataaacggga ggggtattct cccggtacag cggataggca atggcgatgt cggacatggg
    43741 atgttgggca actgcctcga agccgccgct gtgacgtgcc tcaacggatg atttgtttat
    43801 ttcggggcgg ttttcgtatt cggaaaatgt actttcgaca gccggagccg ttcgggacgc
    43861 caaaggaagc tgaacggtgc cgacggggct cgccggccgt tcgacggacg tcatccgtcc
    43921 ggtgtttttt ttctgtctta tcgtttgctg cccggtttgc acggcttttc ccgtgtcggc
    43981 ttttttattg tcgaaagaca cccagaccat attcaaaaca tttcggtcag acggcgccgg
    44041 cgcaccaccg gacaactcca acccgataag aaccgctgcc gccgaaaaat gaatcagcac
    44101 ggaaaaagcc aaggcccaaa tgagggcggt ctgttgatga gcggaatgcc tgtatttcat
    44161 tttttccccg aacgattacg ctttgattat tttttcaaca acttttacgg cctgaacacc
    44221 gtcacgggca tagaacgcgc cgatggattt tgcgtattga tccgtaacca ctgcaccgcc
    44281 gatcaggaag tctgtgcgga ttccctgcgc gcgtgccagc gcaataacct ccttcatgtt
    44341 gaccatagtg gtggtcatca gcgcggaaag gccgatgaca tccggttttt ccttttttgc
    44401 agcatctaaa atagcctgtt cggacacgtc tttgccgagg tcaataacac gataaccgta
    44461 gtttctcaac agcagcgcaa cgatgttctt cccgatgtcg tgaacatcgc cgcgaaccgt
    44521 cgccatcaga acggttcccc tggtcgttat tgtcgctttc tgcaaatacg gatcaaggtg
    44581 cgaaagcgct tttttcatcg cttccgccgc cgccatcaac tgcggcagaa aataaatttt
    44641 tttctcgtag agatcgccga cacgcacaat ggaggggatc atataatcat cgaccagttg
    44701 agacgccgtt ttgccggatt tcattgcttc gtcaagcaat gcggtgatgt cgtctttgtt
    44761 gccttccaga atggcctgaa caattttttc tcccgctgaa acggctgtcg aagggagtcc
    44821 tgcgacagcg ggagtctgct gcgaaaaata gttgatgaag tgaaacgcgg ctctttcccg
    44881 ggcggtcaag gcgtccccgg catttttaac attcatgaac tcgacgctgg ccggattggc
    44941 aatggccatg gtcagcccgc agtgttgagc catcgccaga aaggcggcgt tgatccaggg
    45001 acggcccggc atgccgaagg aaacattgga aaggccgaga atcgttttgc actggaaaat
    45061 gtttttacac cattgaatcg tttcgagcgt ttcccgcgcg gcgtcggcat cggacgcaat
    45121 ggccatggtc aggccgtcga cgacgaaatc ctttttcgta aaaccgaacg cttgcgcttt
    45181 gcgaaatatc ctgtcgatca cgacgcgccg ctttttcgcc gtccggggga tttccccgcc
    45241 ggtcagcggc agcaaaataa acattgcgcc gtatcgggcg gccaggggca ggagcctggc
    45301 cagtttttct ttttccccgg agatggaatt aatcatcatt cttcccggat agagtctcag
    45361 ggcggcctca atggtttcaa tcctggacga atcgatgacc agcggcagac tgctgacggt
    45421 ggacaacaga ctgatcacgt tccggatcgt ttgaacctca tcaatgccgg gctgaccgac
    45481 attgatgtcc agaagaaccg cgccctggtt ttcctgatcc agggccatct gacggatgat
    45541 ggataatttg ccctccaaga gttcctgctg cagcgatttt ttaccggtgg gattgattct
    45601 ttccccgacg atatacaacg ggtcattgtc tttaatttcc aggaatctgc gtgccgagct
    45661 gagcgcacaa acggattttg taagcggcaa aacgggtttc ttacgcgtga ccgacatcct
    45721 gagcgcgcga atatgttcgg gcgttgttcc acagcagccg cccagcatat tggcgcctgc
    45781 agccgccagc ttgacgctga aatcggcaaa tgtcgcggga tccatttcaa aaacggtttg
    45841 accgtctttc agaagaggca tgccggcatt gggcttggcc acaagcggca ccgtggcgta
    45901 cggtttcatc gccgagatga aatcaatcat ttgttccgga ccggccgaac aattgcagcc
    45961 cacggcgtct gcgcccagac tttgcaaagt gatgagcgcg ctttgtggcg gtgttccgcc
    46021 cagcgtgtgg ccgttttttt cgtacgtcat ggatgcaatc gtgaaaacgt ccgcaagttc
    46081 cttgacggcc agcagcgccg cgcgcgcttc ctgaatgtca atcatggttt cgatgacgat
    46141 cagatcgcat ccgccgtcaa ttaatcctcg ggcctgttcc ttgaacgcat ccactgcttc
    46201 ttcaaaagcc agcgtgccga agggttcgac aaacaatccg gtcggaccga tgtctccggc
    46261 gacgagtgtc tgattgccgc aggcttttct ggccaattgt gccagtttca gattgacggc
    46321 gtaaacatcg tgctttgcgc cgaactgttt gagtttatag cgattggctc caaaggtgca
    46381 ggtataaata atctgtgcac cggctttctg atacgatttg tggacagcgc cgatgatgtc
    46441 cggattgtcc agacaccata tttccgggca aacacctgcc ggcaggccca gcttctggag
    46501 ctctgttccg gtagcgccgt ccagaatcat aattttcttt tttaaaaaat ttcttatatt
    46561 aataatttca accacggcta tctcctgaaa tgcaaagaat cagtttccgg tgatcttata
    46621 catcaatggc tgcttttctg caattttcat ttcgttattt gacggatgtg atggatgtta
    46681 tgccagaatg aagcctttat gacgaaacaa gatcagacaa acatcggcca catgctcatc
    46741 atacagaatg cctttgttct tttcgatttc ttccagagcg gcctctatcc ccaacgaagc
    46801 gcggtagggc cggtgagagg ccatggattc aacaacgtcg gccacggcaa gaatgcgagc
    46861 ttcgataaga atatcattcc ctttcaagtt tctgggatac cccgatccgt ccatcctttc
    46921 gtgatgctga taaacaattt ctgccagcgg ccaggaggat tcgacattct tcaatatttc
    46981 atatccgctt cgcgaatgtt cctgtatcag agagtattcg agtttggtca gcctggtcgg
    47041 ttttgccaga atctccgccg ggacggataa tttcccgatg tcatggataa cgccagccat
    47101 ccggaggccg tcgattttat cctgggataa tctcatttcc gcggcaatgg cccgggcaat
    47161 gtctgtcacc cgcaactgat gacccgcggt gtagggatct ctcatctcta tggcggacac
    47221 catgacctgg atggtggtgc cgaccgattt tctcagactt tccagagtcc gtttcagttc
    47281 gatgtccatt aatttgcggt ctgtgatatc ttgcaggata aaaataacac attcctgttc
    47341 gtcgatggtg atcgccctgg cggagtaaat accgaaacgt tcggagccct ccctgcgccg
    47401 gaatttaaac tcccgatcca ggatatccct gcccgttttc aaatccgcga tcattgcctt
    47461 cctctctgtt ttgtccagcc agatgttcat gggcggttcg aacgatttgg ttccgatgac
    47521 ctgttctctt ttccatccca caatcttttc gaatccttga ttcacatcga tgatcagacc
    47581 gtcatgcagg cgggcaatgg ctatgcagtc cggcgtggtc ataaaaattt tatagaattt
    47641 ttcctccgat tggtgtgaaa gttcttccgc cagcttgcgc tccgagatgt tcaataaaat
    47701 tccatcgaaa atgacccggt ccggcaggcg tgtcggcacg gatagcccct gaaaccagat
    47761 aatctttccg gattggatgg cgacgcgacc ttcaaaattc cagcggctgg ctgtctgggc
    47821 tgcctgggta atggatgaca gaaatcgctc ccggtcatct tcatgaatac gggaaaggaa
    47881 ttcaggaaac accgtatcca aatggtccgt atcgaacttt gacaatattt ttgcaaattc
    47941 atcgatcggt tcactgaggt aggttattcc gtatttgccg ttctgtgttg cataaaactg
    48001 aaaaataacc cccggaagat tttcagtgat gccgcgcaat cgttcatctt tttccctcag
    48061 cgctttttca atttgcttgc gttcggtaat attacgacac acattgacaa cggcaaacgg
    48121 tgttccgtct tctttgcgca aaacatcgcc gttgatttca aacaggattt tccgtccgtc
    48181 tctggtcatc agatgatatt ctctggggcc cagtctgctt tccagcatga ggtgcagatt
    48241 gcgaatgact ctctcgcgat cctccggtgc aacgaagtta agcagattct gacccagaaa
    48301 ttcatctttg ctgtaaccgc cgatccgcag cgcattctca tttgcaaaaa gaatattgcc
    48361 tgccaggtcg gtgcgaacaa caacatccgg aattgcattg accagtttga tgtgcagttc
    48421 ttcacttttc cgtaacgctt cttccgcctt tttgcgttcc gtaatatcgc gtgtaatccc
    48481 tataatgcca atgggcttgc catcatccga ccacaggaaa gaagcggtaa cttccgccag
    48541 aaaagtggtt ccgtccttgc gcaactcttc cgattcaaac gtaagcgtcg tatttcgatc
    48601 gaaatcgggt tcagaagacc gggccaactg ttctgccatc attgccgtat ctttggccag
    48661 ttgttggggt gtgaccatat cctgcagggc aagcttcaga tattcgtcaa ccgtccagcc
    48721 gtgtgtgcgc tccacgactt cgttgacata tgtaaaatga cccgccaggt ccatggtcca
    48781 gatcaaatcc ggtatgcagg tggtaatctt acgataccgt tcctcgctgc ggcgtaattc
    48841 ttcctgtgcc gcttccttct gggattctgt ctgcttgtgc tttgcctgaa tttttgtttc
    48901 gttcagctct ctggcaatga ccgggcatag gcgggaatag ttctccttga cgatgtaatt
    48961 gcgagtcccg ggaaagcggc attcgatagt tttatctttg ctgctggcgc cggagatgac
    49021 aatcagggga atgtcggttt tttcttcctc gagaagagcc atggccgcga cagctccgaa
    49081 tttgaggtaa ttgctgtcac aaagaatgat atcccatggt ttgtcccgga gagcttgccg
    49141 catcgcggca gcggtttcta tccgttcgta aaccggatgg tatccgcctt tttgaagttc
    49201 gctcacgatg cgcgacacgt ccttgtccga atcgttcagc attaaaacct gaaggggctg
    49261 cggattgccg gttggagggg cattattttt tttcatacac ctgttcccct gggtgaaact
    49321 ggagtaagga tcggactggt tagaattcta tcagttgctt tttgttttgc tgattgttat
    49381 cagccttgat gaggcatccc ctactggtaa acagcataag cttttcatgt ctgtttgtca
    49441 attgtgtaat cgtcggagaa tcaatcttgg cactgtaagg aagaaccttt aactttttgt
    49501 tgtgcgtggc attttaacgg cagattccgc caatggcggt gacggatttt tccggtatga
    49561 gaataaaatt gggattgatt tttacgccgg cgttctgcat gtgtagctgt ttataaatga
    49621 ttccctggtt ttccagatga aagtcaccgt agccggcgga aaaacggcgt ggcaaaagcg
    49681 tttggccttc ccgcatcaga aggtgattca gataattcat gatccagtcc agtgcggcgt
    49741 ccgtcatttc gctggcggtg gcatcataaa cgaccgcggc ggccagatcg tcctgccggg
    49801 tttttgcctt gatggcgtcc atgacggcat gccccgcagt cgctcccatg aaaacggcct
    49861 cccggcattc gcgaaggaaa atggacagat gctcgctgct gaaatgtacg ttgttcgata
    49921 aaaaaatttg tttcccgtcg ttctttgtga cggccagccg caggtacatc cccttgagga
    49981 caataaggga cgcggcttcg cgaatgaaaa ggtcggtttg cttttgctga tccggcgaga
    50041 cggcggttat tctcttttta aaaccgagcc gctgatatat tttttcgcgg ggaggttcaa
    50101 tcgaaatttt ttccagaaaa atgacggatt ccatgctttc cccttttgct caacgcgaag
    50161 ccggccttta agggcgaatc ccggccaacc gtttgttaag attcccggct accgcgtctc
    50221 ccattgctga agtgctgaca atttttttgc ccggttgatc aatgtcgcgt gtccggaggc
    50281 cttcctgtaa ggtttccgta acggcatttt ctatcagggc ggcttccgcc ggcattgata
    50341 aagaatgccg cagcatcagt gccaccgaca aaatagcggc cagcggattg gcgatgtttt
    50401 gtcccgcaat gtccggcgcc gacccgtgaa tgggttcgta caaggctgtt ttttccccca
    50461 gactggctga cggaagcata ccgatggatc cggtgagcat ggcggcttca tcgctcagga
    50521 tatcgccgaa caggttcgtg gtgacgataa cgtcgaactg agacggatca cggatcagct
    50581 gcagggcgca attgtcgaca tacatgtgtc tcagcgtgac atcggggtaa ttgcggccta
    50641 cgtcggtgac cacgttccgc cagagttcca tcgtttccag gatattggct ttatctacgg
    50701 agaggaggct ctttctgcgt gtacgggcta tttcgaaagc gcggacggcg atgcgacgaa
    50761 tttcatcttc tgaataaatc atcgtgttga cgcctttgcg ttttccgttt ttcgatgtct
    50821 taatgccgcg cggtttgccg aaatagacat cgcctgtcaa ttcgcgcaca atgataatat
    50881 cgatgccgcg gatgatgtct tttttcagtg gcgacgcatc caggagttct tcaaaaacaa
    50941 cgatcggcct caggttggcg taaatgccca ggctgctgcg caagcccagc aacgccctct
    51001 ccggccggac actgtaagcg agattttccc actggggacc gccgaccgct cccagtaaga
    51061 ccgcgtcgct tttcagggcc agtttcagac tctcctccgg cagaggcgtt ccataacggt
    51121 catacgccgt tccgccgaca agtccttctg tgatttgaaa gtcgacgttt tttttgccgg
    51181 aaataatttt taaaaccttc agggcttcgt ttataatttc ctgtccgatg ccgtcgccgg
    51241 caaagacagc gattttaaaa ttattatttt tcattttttt ttcttttccc agcgatgtgt
    51301 tgcatcagac cgccattggc gataagttct tccatgaacg gcggaatggg gttgatttta
    51361 aaaaatttat ccgtgccgtt tatggttatg atgccgcgtg cactgtccac ggtgatttca
    51421 ttcccttctt tcacggcatc gacaatatct ttcgattcga atatgggcag ccccatgttg
    51481 aaggcattcc ggtaaaaaat gcgggcaaaa cttttcgcga cgacacaggg aacgccggcc
    51541 gcttttatgg aaatgggcgc gtgttcgcgc gacgagccgc aaccgaaatt ttctccgccc
    51601 agaatcatat cgcccttttt catctttttc atgaagtccg ggtcggcatc ctccatacag
    51661 tgaaccgcca ggacttcagg atccgacgtc gtcagataac gcgccggaat gatcgcgtcg
    51721 gtatcaatat tatctccgaa tttccagact tttcctttaa atatcatgtt aaaatccttt
    51781 ttaacgagac tcaatcttca gaaacaatgt gtatagaaga ttgtaagttg taatgagtct
    51841 taaattttaa tgaaactcag atattacagg cgtagcgtag taatatctgc aagttgaatt
    51901 atcctacgtg cgaagctaag gggcatgtgc gctttcttac aattcttccg gagaggcaat
    51961 tcttcccagt acggctgaag cggcggcaac cgccggattg gccagataca cttcgctttt
    52021 ggtatgaccc atgcgaccga caaaattccg gtttgttgtc gcaaccgctt tttctccttc
    52081 cgccaggatg cccatgtgtc cgcccagaca ggcgccgcag gaaggcgggc tgatgacggc
    52141 gttcgctttc aaaaaaatct caatcagccc ttcttttaac gcctggcggt aaatttcggg
    52201 ggttgccgga acgatgatca gtctgacatt cgaggaggcc ttcctgtttt ttaaaatttt
    52261 tgcggcgatt ctcaggtctt ctatacgtcc gttggtgcag gacccgatca gggactggtc
    52321 gatttttacc cgtcctgctt tgctcactcc tttgacattg gaaggcaaat gcgggaaggc
    52381 cacctgcggt tcgatggtcg ccgcgtttat ttcaagagtg tcggcataaa cggcatcggg
    52441 gtcggaagaa taaaatgtat aatgtcgttt tgcctttttc cggatgtatt ctgcggtcac
    52501 ttcgtcggga gcaaagatac cgttttttgc gccggcttct atggccatat tggccatgga
    52561 aaatctgtcg gccattgtga ggcgggcaat cgtctctccg gtgaattcca tggccttata
    52621 caaagcaccg tccacgccga tgcggccgat gatatgcaaa atcaaatctt ttcccgagac
    52681 ccatttctgc agtttgccga agatgatgaa tttgatcgag ggcggtacct taaaccacag
    52741 ttctccggtg agcatggctg ccgccaggtc ggtgctgccg acgccggtgg cgaatgcgcc
    52801 cagagcgccg tacgtgcagg tatgactgtc cgcaccgata accaggtctc cgggcagaac
    52861 gatcccctgt tcaggcaata aggcatgttc aacaccgaca aggccgcctt cataaaaatg
    52921 cgttatcttc tgttgcgagg caaattcgcg caccgctttg cattgctggg cggaattaat
    52981 gtccttattg ggcgtaaagt gatccaggac gagagccacc ttatgtctgt tgaatacttt
    53041 ttccccgccc gcctgcttga acgcgttgat ggctatggga gcggtgacat cattgcccag
    53101 ggcaatatcc actctcgcgt tgatcagatc gccggttgaa atttttctca ggccggtgtg
    53161 cgccattaat attttttccg taatggtgtt gcccacgagg ggttctcctt gtaaaaagtg
    53221 aaacggtctt tagcgtattt taattcatta ttcaacaatt ttaacctttt taatattatt
    53281 ctttaaaaat tccatgcgat tcaaggcgtt gatataagct ttggcggacg caacaatgac
    53341 atccggatga gcgccgcgtc cgacgaccga gtaactgctg tgcttgagtt gcacggtgac
    53401 ctcgccctgc gcatccgatc ctccggtgat ggcgtttaca acgtacttca tcaacggata
    53461 gttggttttg gttatgttgc gaatggctgc gaaggtggcg tcgaccggac cgttgccgaa
    53521 acccgctttc cgcacgatgt cctgacccac ctgcatttcc atggttgccg tgggaatggc
    53581 cacattgccg ctgaccacat tcagataaag caggctgtat ttgtcttcgc cccggtagat
    53641 ttcttcggac agaatgacct cgagatcttc atcgtaaata tcttttttga tatctgccag
    53701 ttctttgacg cgagccgcca ccttgttgat ctgctcatca ttgagttggt atcccatctt
    53761 tttcaggtgg tgtgaaatgg cgttgcgtcc cgaatgcttt cccaggacaa tatgtgtgtc
    53821 gctaataccc acggattgcg gcgtcatgat ctcataggtg attttttcct tgatcaatcc
    53881 atcctgatgg atcccggatt catgggcgaa cgcgttggcg cccacaatcg ctttgttggg
    53941 ctgtaccggt atgccggtaa tctgagtcag aagacgcgag gtcttgtaaa tctgattggt
    54001 cttgatgttc gtgtgaaggc cgaagagatc cttgcgggtt tgcagtgcca tgacgacttc
    54061 ttccatggcg gtattgcccg cccgctcgcc gataccgttg atggtgcatt ctacctgccg
    54121 cgcaccctgt ctgatggctg ccagtgaatt ggccacggcc agccctagat cattatggca
    54181 atgcgtggcg atgatggctt cgccgatatt ttggacatgc gcgaagagat aggcaatcag
    54241 attgccgaat tcttccggcg tggcatagcc gaccgtatcg gggatattga cggtgcaggc
    54301 tcccgcgtca atcaccgccg aaaccatttc gaccaggtaa tccttgtccg aacgggtggc
    54361 atccatcggc gaaaattcta cattatcggt ataagaacgt gccagtttta ccgcggcaac
    54421 cgcttctttg agtacctgct cgcgactctt tttgagctga tacttcagat ggatgtcgga
    54481 agaagaaata aaagtgtgaa tgcggggatg tgcggctccc ttgatagcct gccaggcgcg
    54541 gtcaatgtcg agtttattcg cccgggcaag acccgccact tgggattttt taatttcctt
    54601 cgcgatttgt ctgacggcgt caaagtcacc ttcggatgcg atgggaaatc ccgcttcaat
    54661 gatgtctaca cccagccttt ccagttggcg ggcaagcatc agtttctctt ccgtgttcat
    54721 gctgtaaccc ggtgattgtt cgccgtctct taaggttgta tcaaaaatgt aaacacgatt
    54781 tttctctttt ttcataatat cttctcctct gatgactata tgaatgatcc cagtaaccga
    54841 aggttcgtgg gcaatgtacc gtaatttccc cgcgtagttt ccatctatcg ggcggtatgc
    54901 cgctttttaa aaaacaaaaa aggccgtggc ttccgcccac ggccttttag gtgatcagtt
    54961 gctttttctc ctagtgatga cctaaaaacg atgggcgggc ggcctgaggt aaaaggcgca
    55021 acaaagcgag agccagcagt aaaggcagaa tgttgattgc tatgaaattc tttttcgctg
    55081 tttccgtcat cgtttttgca attccaaaaa ttttggaggc tttataatgc aaaaaaagaa
    55141 tcttgtcaaa attttttttg aattcattaa aataaaactg acataaagaa ctgataacaa
    55201 atgcactccc aaagcggtag atggcgttta acaggttacg gatgatgctt tttccccacc
    55261 ggatgatttt ttaatgctgg agatatgtca ataatacatt tgcatgtaag aaagaaatat
    55321 actaagagga cagcacaata aatttttatt cattcgttac atgtaaagaa caatggctac
    55381 aagcacggat ctgttcttga gcaggttttt tacatcaatt acaaaagggg ctatttaaag
    55441 atgaagaaat tttttttatt accgctggct gttttttttc tcgttgcagt tggtttaaat
    55501 tcctttgccg aagaaaaagc acttacgttg tctgcttctg aaaaactgct ttttaacttt
    55561 gattttattc ctgtcgtatt aacggcaagt cccgacggga aacgtattgc ctatgccacc
    55621 cgtgtggaag gcaagcagtt tgttattgtc gacggcaagg cgcagaaacg ctatgacggt
    55681 atcggttcca atgttgtttt cagccccgac agcaagcgtt ctgcatatcg agcccaggtt
    55741 ggcaataaac aggttgtggt aatcgacggt caagaaggga aacgctatga cacagtggga
    55801 tcaaatatcg ttttcagtcc ggacagtcaa cgggttgcct atgccgccaa aacaggcaag
    55861 cggtgtttta ttgtggtgga cgggaaggaa ggcaaatcgt acgagggagg tatcggctcc
    55921 aatattattt tcagtccgga cagtaaaaaa atcgcttacg gggtgctatc agataaaaag
    55981 cagtttgtcg ttgtcgatca acaagagggc actcattatg acagtatcgg caataatatc
    56041 gttttcagtc ccgacagtcg ccggatggct tatcaggctc agtcggataa aaaacgtttt
    56101 ctggttgtcg atggacggga aggcaagcgc tatgatgatg tcagaggcga ttttgttttc
    56161 agtccggacg gcaagcaggt agcctacgtg gtaaaatcag ccggtaagtg gcaggttgtc
    56221 gttgatgagc aggaaggcaa atattatgac ggcatcggag atgatatttt cttcagtccg
    56281 gacagcagtc ggatagcgta tcaagcgcaa tcggacaaga aacgttttct tgtggtcaat
    56341 ggacaggaag gtaaaggata tgaacgtatc ggatcaacca tcgttttcag tccggacagc
    56401 aaaaaagtgg cttatatcgc tcagatcgtg aatggcaagt ttgcgtccgt cattgacggt
    56461 caggagggga aaccgtacaa caatatcggc aacgggtctc ttgttttcag cccggacagc
    56521 aagcgtgtgg cgtatgcagc ccagtcagga cagaaacggt ttgttgtcgt tgacgggaaa
    56581 gaagggaaac actatgatgg agtgggaagt tacacgctcg ttttcagtcc ggacagcaca
    56641 aaaatagttt atgttgcgca ggcagaaaat aaacaaatag ccgttgtaaa tggaaaggcc
    56701 ggcgaaaaag aggtcaataa atataacagc atcgttgctt tggggaaaaa aggttttgcc
    56761 tttgagacat cagacagatt ccgttttctt gccgtaaagg agaaatccgt ctattctatt
    56821 gaagttaaga taaattaaac ggactcaggg ctggaaaatc aatattcgtg tcagaggaag
    56881 atcggatttt atttcaataa agtttaaaaa atgttaaact ttttgtctat acgggatttt
    56941 tccacgcact aaatttacct gcatgttttt aaaaaaattt acttgacaaa aacatctata
    57001 aaaattagat tgcgaaattc gcttgagggt gtaggtgtgt ttttttacat tctgatgcgc
    57061 aagttattga attttatatt ttttgatggg attggctggc gtagctcaat cggtagagca
    57121 gctgatttgt aatcagcagg ttgcgggtt
//
LOCUS       DAOOLB010000007        56187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_384_length_56187_cov_46.809592, whole genome shotgun sequence.
ACCESSION   DAOOLB010000007 DAOOLB010000000
VERSION     DAOOLB010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 56187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56187
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_384_length_56187_cov_46.809592"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(113..628)
                     /locus_tag="PLF77_01765"
     CDS             complement(113..628)
                     /locus_tag="PLF77_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07610.1"
                     /translation="MEQNVFKIKKAFLLPFIAIIILLFVLLILNLLNGQPWEKIILAV
                     AFAVTLLVGIEAANRHITASNEGMKVKKFSRVKTLTWPDVTHLGVVAILNKAYFLLTT
                     TKGFYFFSNMYENHALLIQSIVDKLEAERVEMEVRNYLEHPRERRSLIVICWITALII
                     SAFIVLKLLSF"
     gene            complement(683..1165)
                     /gene="moaC"
                     /locus_tag="PLF77_01770"
     CDS             complement(683..1165)
                     /gene="moaC"
                     /locus_tag="PLF77_01770"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HPC07611.1"
                     /translation="MSKKLSHINHEGNVQMVDVTAKPQTAREAKARGIVRMDAATLTL
                     ISKGRIAKGNVLTTAKIAGIMAAKKTGDLIPMCHPLELTGVDLTFTVDEKKSAIFIEA
                     SVRIAGKTGVEMEALTAVSVAALTIYDMCKAVNKKIVISDIMLLEKSGGKSGTFIRSK
                     "
     gene            1386..2159
                     /locus_tag="PLF77_01775"
     CDS             1386..2159
                     /locus_tag="PLF77_01775"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPC07612.1"
                     /translation="MAALRVRVKDSTDKHKILSLLNKLHPVSCDKPLIRLGAFGDGGY
                     LVPDDLTGIEACFSPGVGFSSVFEKDCADRGMKVFMADGFVNHPSLSHELFVFIKKYV
                     GNTDNDHEMTLDAWVNSSLADATGDLLLQMDIEGAEYDVLSAVSEDLLKRFRIIVIEF
                     HLLDGLRRQPFFNLASQVFEKILRTHTCVHNHPNNCSDAVKVDDIDIPMVMELTFLRN
                     DRIHNPSFVRTFPHPLDCDNTDRNPSLVLPKSWYRNHQK"
     gene            2278..3807
                     /locus_tag="PLF77_01780"
     CDS             2278..3807
                     /locus_tag="PLF77_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPC07613.1"
                     /translation="MFVKVNSMSVIGMESYPVFVEVDTSQGLPQFATVGLPDASVKES
                     RDRIKAAIKNSGYHFPRSHVTINLAPADIKKEGTGFDLPIAVGILAAEKLIREERLRD
                     CLFVGELSLDGSIKRVNGLLSVAFKALETGLTSVFVPEENAPEAAMVKGVNIIPVKTL
                     PDVVECLEGRKTIEPLCLDLEKIFHKTNQYDVDFSEIKGQKQAKRALEIAAAGGHNVL
                     MLGPPGSGKSMLAKRLPTILPDLSFEEAIDVTKVFSVAGFLDKDKTLMAVRPFRSPHH
                     TISDAGLVGGGQMPRPGEISLAHLGVLFLDELPEFRKNVLESLRQPLEDGMITITRSS
                     VTASFPAKFMLVAAMNPCPCGYFGDRVHSCRCTPQQIRQYQSKISGPLLDRIDLHIEV
                     PSVKYRALSDKETGESSAAIKQRVSNARNIQNERFGTNGIMCNARMTEKHIRQFCQVD
                     EEAHKLIDMAMEKLGLSARAVNRILKVSRTIADLEHDESIRTSHVAEAIQYRNLDRGL
                     I"
     gene            3894..4988
                     /locus_tag="PLF77_01785"
     CDS             3894..4988
                     /locus_tag="PLF77_01785"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPC07614.1"
                     /translation="MEIKIKKVPLKKRKTKPADESKLGFGKIFTDHMFTMKYREGEGW
                     YDPVIEPYHPLQLDPTAMCLHYGQEIFEGLKAYRGQDSAIYLFRPMENIKRMNASAER
                     LCMARIDEDLFMEAMKKLVLLEKDWIPHGEGTSMYIRPTMIATEAALGVHPANEYLFF
                     IVVGPVGAYYPQGFSPTKMYVSEEYVRAVRGGIGHVKAAGNYAASLYASRIATNMGYT
                     QVLWLDSVEKKYVEEVGTSNIFFVIGDELITPPLGGSILPGVTRNSVITLARYLGTNV
                     VERPISIEEVIKTSENGSLKEAFASGTAAVISPVGNIYFRDKEYAINGGKTGALAEKL
                     YNEILKIQYGMVEDPFGWRIKIANGKKRSR"
     gene            5193..5957
                     /locus_tag="PLF77_01790"
     CDS             5193..5957
                     /locus_tag="PLF77_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HPC07615.1"
                     /translation="MKQDINSAKKNVPEKIDFLQKMMKFFLNRNKLFTSSWIALSVIV
                     VAISIPGSSFSEERPAIDDVVQKLQLSYESTKDLSADFIQETTVKSIKKTQREEGRVF
                     FKNPQNMSWNYTKPQGKKLVMNHQTAWLYLPQEKVAYRQSAESIFQSRLLIDFFSGSG
                     KLKDDFIITYDSAISPDKDGHYLLVLTPRQKNTTFNVLRLTIDKNNFYILRLSFDDAF
                     GNSTDLRFSRIQFNTGLPKTFFLFKPPKGVQVFEMP"
     gene            complement(6001..6615)
                     /locus_tag="PLF77_01795"
     CDS             complement(6001..6615)
                     /locus_tag="PLF77_01795"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPC07616.1"
                     /translation="MDIDIREIYDRFAQTYDKNRYLFDMTDVMDDFFRRLPSIKGSLL
                     DLGCGAGEPFPAYFIQKEWQVTGVDFSSKMLELAHRYQPAMKTIAADIREVEFPGEQF
                     DAVTTIYCLFHIEYEKHEGIFRKIYRWLKPGGRGLFTYATKAYTGADSFSGYRKFMGE
                     RLFYSHTTPEKLRGILETIGFKIDAFQYRSIGGETFLWVTIAKP"
     gene            complement(6625..7554)
                     /locus_tag="PLF77_01800"
     CDS             complement(6625..7554)
                     /locus_tag="PLF77_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC07617.1"
                     /translation="MNLEEKIFLLQEIFKDCILCPRNCHVNRTKNERGYCGLAASIIM
                     DCALAHHGEEPPLSGTHGAGTIFLSSCNLGCIYCQNHQISHHITGQEMNSRQLADVML
                     DLEKRQCHNVEPVTPTSQMPLVMEALLIARKAGLKIPFVYNCGGYESPDILKRLDGMV
                     DIYLPDFKYGQEEDSMMFSAAPGYPWFAADSIKEMVRQVGDALELKNGIARKGIIIRH
                     LVLPGRIENSKAVLRLISRKISNNVFLSLMSQYTPMAKVRNHPDLKRRITKKEYEQVL
                     DYAEKLGFHNLFIQEVSDFDLTPDFSLDNPFKS"
     gene            complement(7586..8653)
                     /locus_tag="PLF77_01805"
     CDS             complement(7586..8653)
                     /locus_tag="PLF77_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07618.1"
                     /translation="MSRIRIKAGRKIYQLIKDGGFNFDLISTYFGPAVGPRWLIASGF
                     DLTLLERCSLGRSTPVQLVGSSAGAFRFAAWLQPEAVKSYRQLMEAYIHVTYTKKDTP
                     ATTLEKITGIINAYLENDALPFALNNKKYRLTIITARSRGLVAFQNVLLQKTGLIFCF
                     LLNYLSRENIYKFSERVVFYNASKPPAFCLKPQFRGIYVPLNEINFKYAVLASGAIPL
                     VIEGVRDIFGAPRGVYRDGGLIDYHLTHPFASGENDIVLFFHHQERIIPGWLDKKITR
                     RAPDPNILNNVLMVFPSQYFTESLPGGKVPDRTDLVTFIDDPQTRIRNWCKAVDLSAP
                     LGDDFLELVESGRLRDVVEPF"
     gene            9130..10386
                     /locus_tag="PLF77_01810"
     CDS             9130..10386
                     /locus_tag="PLF77_01810"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HPC07619.1"
                     /translation="MDIKDSVEHIARNAALAAKKLSRVTETEKNTALLRMAEALERNS
                     KFLLDENAKDVNGAKKAGISKAMLERLTLKQSTIDQMAKGLREVATLPDPVGKIISMW
                     RRPNGLLVGKMRIPLGVIGIIYESRPNVTVDASALCLKAGNAVILRGGSESIKSNLAI
                     ASVLRKVLQDTSIPVEAIQVIPVTDREAVAAMLKMEEYIDLIIPRGGEDLIRAVVAQS
                     RIPVIKHYKGVCHIFVDASAEMAMAVDICLNAKTQRPGVCNAMETLLVHQTIAGKFLP
                     VVANKMKKAGVVIRGCEKTKSILKRIEKAVEDDWSAEYLDLVLAVKVVENMNEAIAHI
                     EKYGSLHTESIITGDYSNAQRFLNDVNSSTVLVNASTRFSDGFELGLGAEIGISTTKL
                     HAFGPMGLEELTTTKFIIYGNGQVRT"
     gene            10383..11042
                     /gene="nadD"
                     /locus_tag="PLF77_01815"
     CDS             10383..11042
                     /gene="nadD"
                     /locus_tag="PLF77_01815"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HPC07620.1"
                     /translation="MRWGLFGGTFDPIHFGHLRGAEEMLEIFNLNRIIFVPSSRPPHK
                     LEADITAFNHREQMIRLAIEDNVNFSFSEVENLRAGKSYSVETVEYILNKYMEDLELY
                     FIVGQDAFQAVTTWKDWERLLLLCNFAVMTRPGYDDMRLDAILPRTFAAKFIYDKKTD
                     GFKGPTGQAIYFRHTSFLDISSSHMRDMVRTGNSIRYLTPGKVRQYIMKHNLYKECRD
                     N"
     gene            11072..11872
                     /locus_tag="PLF77_01820"
     CDS             11072..11872
                     /locus_tag="PLF77_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPC07621.1"
                     /translation="MNYKFIELEVKDHIATVTLAREKSLNAVSLELASEITDVFRKLS
                     AMDDVRVVILKSRARIFCAGLDLKDAAGGFTGTTRAPWDMIKKSQPLFDCCNVIEECP
                     KPVIAAVHGQCIGAGLHIISACDIRLCTQDATFCLKEPKIGLCADMGALQRLPLIVGQ
                     GYAREIAFTVAFYNAQQVEKMGLVNHVYADQAALYEAAREMARQITENAPLAIECTKE
                     VLNFSRGTSVSEGMSLAIQKNMFLLATDDLKEAFVAFMEKRQPDFKGR"
     gene            complement(11964..13973)
                     /locus_tag="PLF77_01825"
     CDS             complement(11964..13973)
                     /locus_tag="PLF77_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPC07622.1"
                     /translation="MAYRTDTLCGIFHNQALRYGDQVELLRAKFDQNGNPSAQWHSRT
                     WKETREEAIGLAKGLMVLGLKKSDRIVIFSESRPRWIIADQAIQACCAIGVPLYPTLT
                     VEELGYMTSDSGSKIVIASTQGRGEMALKAKAQGVPIEKIIVMCSWEGAKPEGVYTFD
                     EVIDLGKGKISDEEIEKNINSVTPEDVTSIIYTSGTTGKQKGAILTQKNWVSNMLQAS
                     NSTLMRKQRELGLHLTFLVHLPMCHVFGRTSDYHVGALQQSGILVFAESFEKIPKNIL
                     EVRPNVLISIPRLWEKIYDTTRSIVSRQDKRVQSIFNWATRLGEKYCEAMATATRLGF
                     LDLLQFALANILVFNKIKKTAGLDHVVLGISGGGKLSKEVCVFIRALGIQLAEGYGLT
                     ETSPVINFNEPEFATSFDISKASWFQKKMLDLTVDLMVTKQAKGLSPYTNPLRSLSLS
                     LAYNTLAHKLQIKPGTVGRPALWTEEKIAEDGEILVKGPQVFQGYWNLPDATKEAFTD
                     DGWFKTGDIGEFDKDGFLTITDRKKELFVTSGGKNIAPHPIELALTAKPYIDQVCLIG
                     DARKYISALVVPDFQELHRFSKHNGIPACSDKDLLANEQVKELIQKQVDEVNEALPRY
                     EQIKYYKLLDKPFSVETGELTPTLKLKRRIVFEKFKDDIESMYKD"
     gene            complement(14102..15091)
                     /locus_tag="PLF77_01830"
     CDS             complement(14102..15091)
                     /locus_tag="PLF77_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPC07623.1"
                     /translation="MNHKNVYLKDIKQGDKVASSFLVTEKNMAFSQKGSPYLTVRLKD
                     KTGEMECKVWENALEFDNVFKKGDVIFIEGRAASYKNSLQISILNIKNIPRQDIDPAD
                     YLPAVKTDVKTMFAELLAFAEKVNHRHLKALLNAFLQEEETAARFRRAPAAKGFHHTY
                     LGGLLEHTLSVVRLLDKVADHYSILDRDMLITGGILHDIGKIYEFSFESIIDYSDEGR
                     LIGHIVMGVEMIDQKIAAINGFPPQLAMEMRHIILSHHGEFEFGSPKRPKTLEALVVH
                     YIDDLDAKFNAFQTFMNESSSPDSDWTVYHRFLERFLYKKKNAAFDIPEDPKI"
     gene            complement(15088..15861)
                     /gene="fdhD"
                     /locus_tag="PLF77_01835"
     CDS             complement(15088..15861)
                     /gene="fdhD"
                     /locus_tag="PLF77_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HPC07624.1"
                     /translation="MNDLQKIIKTIRCFSGSYSAVSGSRVKVIQEVMITVRVNKTPLA
                     NIACAGIHLEELAAGFLRSEKIITRRQDIENISLAKNRVDVLLKNKNFFPDVSVKNIA
                     SSGARGRSNVALLTPLNVPAGLSINTALALKLMDDLLNGSAMHNETGGTHCSALAFRK
                     KIAALREDIGRHNTIDMLGGYALLNKLNLHRAIILTTGRISSEIVYKIWNMGVPFIIS
                     HSAPTANAVTLAKQANITLIGYVRKGRMSIYSHERRVII"
     gene            complement(15858..17771)
                     /gene="selB"
                     /locus_tag="PLF77_01840"
     CDS             complement(15858..17771)
                     /gene="selB"
                     /locus_tag="PLF77_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPC07625.1"
                     /translation="MKHFVLGTAGHVDHGKTALIKALTGIDTDRLKEEKQRGITIELG
                     FASLKLPSGLTLGIVDVPGHEKFVKHMVSGAAGIDLVLMVIAADEGIMPQTREHLHIC
                     SLLGISRGIVALTKIDLVEKDWLNLVESEITDFLRGTFLESAPVIPVSAIKQEGLSEL
                     LQALDTIIAEITARTDDGIFRLPVDRIFTMKGFGTIVTGTLVSDHLKTGEDVQILPQN
                     ISARIRGIQVHNRPEEEAWAGQRTAVNLQGIEKSAIVRGNVLVRPHTVWPTQRLDIAV
                     DYLSANTKSLKNRALVRLHTGTNEIISRIILLDTDELAPGQQGYAQLILSEKDVVVAG
                     DHFVLRSYSPVTTIGGGVIIDPLPQKHKRFNRGAIEELMLLQKGTLPEKITVILDRTG
                     FKGTPLQQLAFRLGMKSRKIREVLDDLFSKKEAFLLDSEDTTVISARFFNRLEEMIIY
                     NLSGYHKKNPLKEGIPKEELKGSLANDISPKLFNMVLNTLNKKETIVSNKDNVRLAKH
                     QVELAGDLDALRQNIIGIYKTAGLMPPSLTDVINKFIDRKNKAQNIVSLMLKEGELIK
                     INEELCFSGEILSKLRNDYKAMLIKDGKATPASFKELTGLSRKYIIPLMEYFDMNKLT
                     VRVGDHRVLREKT"
     gene            17938..18369
                     /gene="ruvX"
                     /locus_tag="PLF77_01845"
     CDS             17938..18369
                     /gene="ruvX"
                     /locus_tag="PLF77_01845"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPC07626.1"
                     /translation="MRILGLDYGEKRIGAAICDELGLTAQGLSTIIRKTKKHDGEILE
                     RLIRDFNVEKIVIGYPLKLDGSEGIQCEKVNRFAALLEKTFGLPVIRWPETLSTKEAE
                     EILINSGIRWGKRKKMVDKLAACLILQSYLDSVAPSLKNNF"
     gene            18442..19557
                     /gene="mltG"
                     /locus_tag="PLF77_01850"
     CDS             18442..19557
                     /gene="mltG"
                     /locus_tag="PLF77_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPC07627.1"
                     /translation="MTSQASAWKSKFHEIFIFLKKIFSGRRWIRRTVLYSVAVILIFC
                     IMLLDYAFSPIDVRDKTVVIDIPTGSSFMEATELLQKEGLITNRFFFYSLAAIKRASR
                     NIRAGEYEINTLTNPITMINKLMHGDIKVYEVVIPEDLSFRDIAVILDKHKLINKEIF
                     FELARDQEFLKSLNIEAESIEGYLFPDTYHFNRSMNTRRIMKKMVDTFWQKMTPSMIR
                     RANQMGLSVHKLVTMASIIGKESGSALEKPSVAAVFYNRLKRGMPLQSDPTAVYDMDD
                     FEGKIFRSHLKRNSPYNTYRIKGLPPGPIANPGLDSLKATLFPSSVDYLYFVSKKDGT
                     HHFSSSLVEHNQAVNRYINLKDEQNQLLEYDIPVEEY"
     gene            19762..20208
                     /gene="mraZ"
                     /locus_tag="PLF77_01855"
     CDS             19762..20208
                     /gene="mraZ"
                     /locus_tag="PLF77_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPC07628.1"
                     /translation="MSVFRGQYHHTIDEKGRIIFPSKFREIFAEEYDNRMVITNWDQY
                     LMVFPYDEWLVIEEKVAHQSKLRKEVRSFQRFFMSGAIDCSIDGQGRVLIPPSLREYA
                     QLEKDIVLAGMLKNIEIWSKDRFEERMKQAVGEIDKCADFIADLGI"
     gene            20212..21147
                     /gene="rsmH"
                     /locus_tag="PLF77_01860"
     CDS             20212..21147
                     /gene="rsmH"
                     /locus_tag="PLF77_01860"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPC07629.1"
                     /translation="MTAEFYHQPVMVKEVIDSLIVSRAGVYVDGTVGGAGHSYAILKQ
                     TDALLIGIDCDDEALRFAEKRLQEFGSRKILLKENFVNLDHILKSLNIEKVDGVLLDL
                     GVSSRQLDASERGFSFSQQAPLDMRMDRSMTLSAYDIVNSFAQNELEKIIRFYGEEKM
                     ASRIARAISKKRQESPIATTTQLAAIVASCMPAGTRWRKIHPATRTFQAIRIAVNHEL
                     DNIKPAMHAAVDVLKTGGRLCMISFHSLEDRIVKNEIRSLEGRCVCPPDSPFCVCQRD
                     AKLKNLTKKAIMPSEEEIASNPRARSAKLRVAGRL"
     gene            21149..21496
                     /locus_tag="PLF77_01865"
     CDS             21149..21496
                     /locus_tag="PLF77_01865"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HPC07630.1"
                     /translation="MAPSAQSADARVVPHLGEVEGASVGIKYPTFIVLAVVLMLVFVA
                     YVGSHIRMTELEYNIAAEISTQEKILEEQKKLKLEYAMLKSPQRIENLVRDRLQMSYP
                     DNEQVIVIRKSGG"
     gene            21498..23474
                     /locus_tag="PLF77_01870"
     CDS             21498..23474
                     /locus_tag="PLF77_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HPC07631.1"
                     /translation="MAIESKKRMKFRIIMIFMFFFVLFVALISRAFQLQVLSGQKLKK
                     LATRQHTTVLQLQPERGIIYDRNGEKLAISIMADSVCADPSKVIDPAKASKQIAYILG
                     LDSQAVYKKLSAPRNFCWLARRISPEQTAAVVKADVEGVFLVKEPKRFYPNGELAAHL
                     LGFVGLDASGLEGLEKKYNDSLVGSPEKLEWARDAKGKKLFLRVEKTQTAKNENTNLV
                     LTIDNRIQYLVETHLKEAVIEKGAKGGLAIVMDPKTGEILALANQIGFNPNNIQGLSP
                     ERWRNRAITDYYDPGSTFKPFLVAAALEEKVIREQDRFFCENGNYRIADRVIHEANRK
                     RYGTLTVREILKYSSNIGSAKIAQKMGKEKFYNYIQKFGFGSKTGIDLPGESSGLVRP
                     VRDWVKVDTSMIAFGQGISVTAIQLISAVSAIANNGVLMKPYIVRGIADQDNRLVKLY
                     TPTVVRQVISPRVAKSVTNMLMDVVGAEDGTGKNAKIVNVAVAGKTGTSQKFDTARGV
                     YSSEKVRTSFMGFFPAKDPQVAILVVLDEPQKDKWGGVASAPVFKNIAEQILNCFKTD
                     IRETPVFKKEKTDKVQLVSAEQTLLPEIVENSDGPSMPNFMGLTIREALKKAKTESIE
                     LKISGNGWAVRQYPEPHAPLGHERVCSVVFELTD"
     gene            23482..25005
                     /locus_tag="PLF77_01875"
     CDS             23482..25005
                     /locus_tag="PLF77_01875"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPC07632.1"
                     /translation="MKLTRLTEGLDMINMSGEASGDVSGVCFSADQCVPESLFVAIPG
                     LKRDGHDFIEEAISRGARFIVHEKVLTFPPFVRAIKVSDSRRALGILAKNYFGDPSAA
                     LTVIAVMGTNGKTTISYLLESILKGAGCKCGVLGTVNYRYNDKVYDAPNTTPESYEMQ
                     KILRAMADDGVTHVVAEVSSHAVDLKRVDDCDFDLGIFTNLTRDHLDYHVTMENYFQA
                     KKRFFAEILPQSRKGYAQKMIINADDDWGRRILKDVPLPALTYGIENDCAVRSLNCKL
                     SLNGIEADIRLAGNNVLIQSPLIGKFNLYNILAAAGAAFILHIPVPVIKAGIENLSHV
                     PGRLESVPSAGGVHVFVDYAHTDDALRRVLQNLSELKKNKIITVFGCGGNRDKGKRAL
                     MGDAATRYSDLTIITSDNPRLENPLDIIADIEAGIDRKDVKKSDPEMLKMNGRARSYA
                     VIPERRKAIEAAIRAARRDDIVLIAGKGHEDYQILGTEKISFDDRVVARQILETKSY"
     gene            25014..26462
                     /gene="murF"
                     /locus_tag="PLF77_01880"
     CDS             25014..26462
                     /gene="murF"
                     /locus_tag="PLF77_01880"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPC07633.1"
                     /translation="MKSESPVFFLNDVLTSTSGTMMSGLPETVFRGVSTDSRSIKKGN
                     LFVALKGDHFDGHDYAESVFQGGAAGILAQDDEKIRRMKVDDTFAVIKVADTLFALGE
                     MAHDWRKKFSIPVIGLTGSSGKTTAKEMIASILGLKKNILKTEGNLNNLIGLPQTIFR
                     LTDQHDMMVLEMGTNTRGEIRRLTRIAEPDIGLITNVGPAHLEGFGSLQVVGEEKGDL
                     FLNMPSSGIIVVNLDDEAILTAAETWKGRKITFSMSPNADVTVFDIERNGAKGVRFNL
                     AIRGNTQKVEMKITGLHHINNAMAAAAVAVAAGMDLKNIAEGLSAFRPFHGRMEIIKL
                     RNGAYLLDDSYNANPASVREALMTLKDLKGNHHAYVFLGDMLELGAQADEMHRKIGML
                     IATIGVKTLFLQGDYAGMTAAGATEGGMASDSILFLSDKENGTDFLKKCLKKGDWILV
                     KGSRRMNMDRIAAQICEDFGRDKVSEENKTDY"
     gene            26479..27558
                     /gene="mraY"
                     /locus_tag="PLF77_01885"
     CDS             26479..27558
                     /gene="mraY"
                     /locus_tag="PLF77_01885"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPC07634.1"
                     /translation="MIYHLLYPLHTTISFFNVFRYITFRTIFAAITALLICLIFGKWM
                     IRKLQDMQIGQQIRDDGPQSHLVKKGTPTMGGMLIIMAVVVSTLLWTNLADVYVWLIL
                     MVTLGFGLIGFIDDYSKLAGKSSRGVSGKTRLAAEIIIALFVSVILYVKPGFNAQVTI
                     PFFKTVLPDLGWGYVLLSTFIIVGAANAVNLTDGLDGLAIGPVVICFATYILFAYFAG
                     NIKTSNYLQIVHVSGAGELAVFAGAIVGAGLGFLWFNAYPAEVFMGDVGSLSLGGALG
                     TIAVITKQEILLAIVGGIFVVETFSVIFQVGYFKMTGGKRILKMAPLHHHFELKGWAE
                     PKVIVRFWIISILLALMAISTLKLR"
     gene            27566..28912
                     /gene="murD"
                     /locus_tag="PLF77_01890"
     CDS             27566..28912
                     /gene="murD"
                     /locus_tag="PLF77_01890"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPC07635.1"
                     /translation="MDFNGKKIVVIGVGKTGVAVARFLGKKSARIVVTDEKSPDHWDG
                     GFKQIAGEPWLEVGEYNSVDVLKDADMVIPSPGIPPGNPLLVEALKKNIAVVSEIELA
                     YRFIRVPIIAVTGTNGKTTTTTLLGEILQKSGKKVFVGGNIGNPLIEYADKNQDADFI
                     VAEISSFQLQWIDQFRPFIAVLLNITSDHVNYHGSFAEYRRIKARIFANQTGKDFAIL
                     NAEDDEQKKIEGNIRARVAGFSSKHVLPSGIFVKEENIVLSLPGMQEEKYPLSMINIP
                     GLHNVENVMAAILAARFCGCDPSDTIRAVENFRGLPHRIEFVEEKNSVKFFDDSKGTN
                     VGSVLRALETFTQPVILLLGGRDKDGDFETLQPVLAAKAKKIVLFGEARERIASLIGQ
                     TVPVIKKTKLQEAVEVAYQEAVPGDVVLLSPGCASFDEFANYKERGNFFRKVVENL"
     gene            28913..30022
                     /gene="ftsW"
                     /locus_tag="PLF77_01895"
     CDS             28913..30022
                     /gene="ftsW"
                     /locus_tag="PLF77_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HPC07636.1"
                     /translation="METAYKKEDNYPDFVLLLVTLLLAVVGTAMIYSSSSIIALEKFN
                     DGQYFLKKQLVFVFLGFTIMVAMTKVPYTHLRKIAYPGMLLCLLLLSLLFVPGIGMRR
                     GGATRWLNLGGFSFQVTEMVKIMIVIFLSHLLTRKAFQITNFTKGVLVPLAATSVVMM
                     LILLEPDFGTTAIIATIMLMMLCIAGAQIKHLALLIGAFIPVGIALILYKGYRLTRLT
                     AFLDPWKDADNTGFQIIQSLISFGSGGTFGVGIGDGMQKLFYLPEPHTDFILSIIAEE
                     SGFIGVAMVITMFALFAYRGFRIALNAPDLFGSLLASGLTMVICMEAFINIAGVMGLI
                     PLKGLVLPFLSYGGTAFIMSMTAVGILLNISTQTS"
     gene            30077..31162
                     /gene="murG"
                     /locus_tag="PLF77_01900"
     CDS             30077..31162
                     /gene="murG"
                     /locus_tag="PLF77_01900"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPC07637.1"
                     /translation="MRIVIAGGGTGGHLFPGIAIAEEFIKRNAQTKVVFIGTKRGIES
                     RLLSRLGYDLETIDIEGIKGRGIRALVKSVFQIPKSLWQARGILKQFSPDMVVGVGGY
                     ASGPAVLAAYLMGIPTSIAEQNAIPGVTNKILGNIAGKIFVTYPRTQQCFPRRKVVIS
                     GNPVRASFAAQSFRTKEDRTHRQLLIFGGSQGAEAINRGVIDMLPRLSGMKDRIRIVH
                     QTGARQMEEVKKAYAQSGLQAVATDFIMDMAEAYAQADLIICRAGATSLAEITVAGKA
                     AVLIPYPWAANDHQLKNALAMSDAGAAVVIPEKELSGESLFNAIDGILRDENRRLLME
                     ENSLRMGKAEAAATIVDECMKLTDAKH"
     gene            31192..32604
                     /gene="murC"
                     /locus_tag="PLF77_01905"
     CDS             31192..32604
                     /gene="murC"
                     /locus_tag="PLF77_01905"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPC07638.1"
                     /translation="MKKDKEKSLMQRKIKCIHFVGIGGIGMSGIAEVLVNLGYNVGGS
                     DVQPSETTARLQKLGAKIVIGHAAENVGNADVVVTSTAVKEHNPEVLEAHRKNIPVIP
                     RAEMLAELLKMKFSVAVSGSHGKTTTTSMISTILAEGNLDPTMVIGGKLASIGSNARL
                     GDGEIIVAEADESDGSFLKLSPTIAVITNIDREHLDYYPGIAEIKEAFLTFANIVPFY
                     GCTVLCNDNIHVREILPAIKRRMITYGIDTPADYSAEDIKFTKRKTCYSLVHKGEKLG
                     KIELIVPGLFNVYNSLAAIAVARELNLDLATIKEGLKSFSGVQRRLEIKGRVGEVTVL
                     DDYGHHPTEIKATLAAMRQIWKDRLIVVFQPHRYTRTRALFEEFTKAFDEVDILILND
                     IYPASEEPIPGITAAALCEAIRKAGHKHVEYIPGAEATVEYLLKTVHQKDTVATIGAG
                     SIYKIGETFITQLVARGKNT"
     gene            32604..33533
                     /gene="murB"
                     /locus_tag="PLF77_01910"
     CDS             32604..33533
                     /gene="murB"
                     /locus_tag="PLF77_01910"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPC07639.1"
                     /translation="MTSHCEIRGILQEIDAGKVLYDEPMSLHTSLAVGGKADALVVVE
                     HENQLARVIRKFRMEGINYFTAGNLTNVIVKDGGYRGAIILMTGMKEIQCKPVGGHEG
                     YILAQTGASLAKVVSRSVVEALSGVEFCSGIPGSVGGAVWMNAGAYGREMKDVIESVT
                     VLDTEGSKKTMIREAISFGYRTSSFPSGTIILGARFRLKKGDRATMKARISEILQWRQ
                     AKHPLEYPSAGSVFKNPPGHPAGKIIEDMGLKGEKAGGAEISSKHANFIVNKGGATAA
                     DVLALIRLVQSRAREEKNIDLETEVVVIGEDRC"
     gene            33527..34366
                     /locus_tag="PLF77_01915"
     CDS             33527..34366
                     /locus_tag="PLF77_01915"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HPC07640.1"
                     /translation="MLKKTGIQTKQNRIRRRFTSLSGDFFTAMTLLSMAVFLSVLFIY
                     VYNVMMSSPYFQIKEISVRGLKELTEKDILASANIKPQQNLLAVNKNSIIRRVCSNHW
                     VETVYVGKELPGKLVLDVRERIPLALVKRGGDFYLMDVKGHVFKRLGGSDEVDLPVIT
                     GAATGEATRSPLLLSALGLIQRISKSPAYAHLGTISEIHIDSVFGLALVSDNGLYLKL
                     GTDDFENKLRKLKAILTDLENRGMKTGFLCVDLSDHSKVTVKRKYVPEKTQDGDQNKN
                     YLI"
     gene            34399..35616
                     /gene="ftsA"
                     /locus_tag="PLF77_01920"
     CDS             34399..35616
                     /gene="ftsA"
                     /locus_tag="PLF77_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPC07641.1"
                     /translation="MGKRSNVLVGLDIGTTKTTAIVGDVTETGIDIIGIGTSQTKEMR
                     KGTVVNIDTMVESIKTAVEEAEHMSGCRINSAFVGISGSHIKGQNSLSIVSIKGREVD
                     EKDVQRVIEASEAIAIPVGRKILHILPQSYVIDGQEGIKDPIGMSGVRLEAKVHIVTG
                     AIAAIQDIEKSVNRVGLDIDGIVLEQLAAGEAVLSSDEKELGVVLVDIGGSNTSVTIF
                     SESSIKHTAILPVGGNYLTSDISTGLRTPLSEAEKIKIKHGCAMTSMIPKDETIRVPS
                     LGGREDREVSRQILGRIVEPRMEEILNLAYNEVVKFGFEDLLAAGVVLTGGTSLLEGI
                     SELAEKIFDMPARKGIPIGVGGLNDIVNSPAHAIGVGLIIYGRNQNILRGTSTGMLGS
                     ITRTVKKMFSEFF"
     gene            35632..36789
                     /gene="ftsZ"
                     /locus_tag="PLF77_01925"
     CDS             35632..36789
                     /gene="ftsZ"
                     /locus_tag="PLF77_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPC07642.1"
                     /translation="MFELTEVCSENLAKIKVIGVGGGGGNAVNTMIASCLQGVDFINA
                     NTDAQALGVSSAPVKIQIGSQITKGLGAGSDPEIGKQSALESRDEIRPYLEGADMIFI
                     TAGMGGGTGTGAAPVIAEIARECSILTIAVVTKPFQFEGKKRNMQAEEGIEQLREKVD
                     TLIVVPNQRLLSLGGRNLSLLEAFKKADDILYQAVKGISDLILVPGLINLDFADVRSV
                     MSNMGMAIMGTGIASGENRAVEAAQRAISSPLLEDNTIQGAHGILLNITGGSDMSLYE
                     VNEASSLIQEEADEDANIIFGTVIDQNMEDEIRITVIATGFDDFARKKNDSVGNVTRF
                     RGVGGREDLATPTFIRRERKLNSGEIAVVGLGDETENMDIQIPTFLRRQAD"
     gene            36856..38544
                     /locus_tag="PLF77_01930"
     CDS             36856..38544
                     /locus_tag="PLF77_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC07643.1"
                     /translation="MDWKLKAVYKSILENETGYVKKIWGKDNTVCLVYPNHYRIGMAN
                     LGFQTVYQLLNAQPSFLCERAFLSVSGNEARCVSGTAGIFSLENQKALTEFDILAFSI
                     SFENDYPNILKIMDDAGMPLRSADRSEKYPLILGGGIAPTLNPEPLADFFDLFVLGEA
                     EDILPVFCRFFEASRRLGQRREQFLKDAQKQLHHVYVPGLYDVHYSPERKITSVVPRE
                     AGLPEKIKVEPVNDINAFRTQEVISASCTEMEDMFLVEVNRGCAHGCRFCAASFIYRP
                     VRFRGIEEMIGAIHDGLTRKNKIGLVGTAVSDHPDIPAVCRYIIAHNGRAGLGSLRID
                     RINEEMVGLIKANGIETVALAPEAGSQKMRDILKKKISEEQIMYATALLAEHGIANIR
                     LYFMIGLPREEESDIDAIIGLTKKIQHHVRLRFDGKKQFRRLTLSINQFIPKPATPFQ
                     WVPLLDVHIAGKRIKKIKEAFRKDKSVRVIHDVPKWNYVQALLALGDRRVGEILLAVN
                     RLDGNWGRGLKEVNVNPDFYVYREKSFDEILPWDIIDLGISRKALINENQKALK"
     gene            complement(38555..39826)
                     /locus_tag="PLF77_01935"
     CDS             complement(38555..39826)
                     /locus_tag="PLF77_01935"
                     /inference="COORDINATES: protein motif:HMM:TIGR04013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04013 family B12-binding domain/radical SAM
                     domain-containing protein"
                     /protein_id="HPC07644.1"
                     /translation="MDGETALIFYYLKGNLNSYNALAGALEKYPDIKNIDVYFLSSEK
                     ELLQKISAISKKHRETIIGISFCTPQFLKIKDLINRVKKLAGETVFLAGGPHPAGAPL
                     ETLKLGFDFVISGEGEETLTELLSKINHDENNSDIKGLSYLDKNGEYVFTGRREAADI
                     NTFYPISHYFSKYGPIEITRGCPYACRYCQVSQLFGTTPRHRSIENILDCVKIMKRQD
                     STYFRFISPNAFSYGSEDGKRINNEKLENLLSDIRRLVGKSGKIFFGSFPSEVRPEHV
                     NAHTVKMVKKYANNDNLIIGAQSGSQRMLDCAGREYDVATIYNAVDVTRQAGLKAKVD
                     FIFGLPGETDEDIASTLNVIQDLSRSGAKIHAHTFMPLPRTAFENETAGKIGPALKKE
                     LNRLCANGIIYGQWQQQEKIAAGIAAKQDNA"
     gene            complement(40071..40316)
                     /locus_tag="PLF77_01940"
     CDS             complement(40071..40316)
                     /locus_tag="PLF77_01940"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPC07645.1"
                     /translation="MADSKSDFFTGLIVGGLIGVALGILFAPKSGKESREDIARKADE
                     LMAKAKEGYEKAAGKYSEMTGVESFRDYKTADNEDLA"
     gene            complement(40745..42133)
                     /locus_tag="PLF77_01945"
     CDS             complement(40745..42133)
                     /locus_tag="PLF77_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPC07646.1"
                     /translation="MTNAKRAEVHAIAMETVKAAEKVPEKYFVVDTSALEYGMDVFEK
                     LRQGGRNCVLIPYATWEELDSHKTGNDFKADIARNVIRNVRNLFRAKDHSVRFFQYDF
                     NLVKDADLKVDKNDHCIIATALNAKKKLGKAKVILVTQDAALEALALNFGLDVQSIKM
                     HQTDIKQHHTTRATINLQRSWISDKTFPLKLPEAKLAAQLEQVLYNEGIICYCDLDGV
                     WGPAFVAVRKGDYFRIIDRDIHLLGIKPRSNNGDVNWGQFIAMDLLRDATVPLVTLTG
                     KAGTAKTFLALLAAFEQASHYRQILVSRATIQLGNKDRLGYSPGDIDAKMKPWMHPIY
                     DNINAIKELVDERMKEKINDWLANGKIEMMDLDKIRGRSIQKCYVIVDEIQNLPPSHV
                     KAIATRGAEGTKMVFTGDFSYDQIDVPWLDERSNGLSVLNAKMAGSRFFGSVHLDQAI
                     RSELTKEILERW"
     gene            complement(42408..44147)
                     /locus_tag="PLF77_01950"
     CDS             complement(42408..44147)
                     /locus_tag="PLF77_01950"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC07647.1"
                     /translation="MKKNLFYKIFFSYLVIICLSFFLLDIFVRDEVRKVLTSQIETEL
                     LSYAQLIDQTSLEKASGHLKQVAQISQSRITFMDARGRVFADSEKDIALLESHFNRPE
                     VQEARLRGTGKSVRYSDSLGIDMLYMAVAVKDAQKIKGYVRLARPLHHVQNVIDKVYQ
                     YILLTLFVVALIALGIALFFSYRLYEPIKIMEQFTEKLRRGDPVGSIILDTSDETKTL
                     ADNINYLVEELREKINIANEEKNKLMTAFTSMNEGVLILDEQGVIEFSSPVLSNMLAI
                     QYDDVVGKTLMEAFRNIDLQKAFQEFKEKRISVSREIILGHADKVVLNVSLSAVHGRP
                     DEEKTMIVFHDVTRLKKLEQVRMDFVANVTHEIKTPLTAIIGYLETIQSGAITNQEET
                     KKFIDIILKQAERLNRLVEDLLTISNIETKQTVLNFESVSLRKAIDTMLSLIEAKAEL
                     KNISVRNNVSDNFPPITANRDRLTQIFVNILDNAVKFTPNGGEISINASESGSYTVVS
                     IQDSGIGVPKDDISRLGERFYRVDKSRSRDLGGTGLGLSIVKHLMIAHGGRMEIESQP
                     GKGTKVSLYFPVK"
     gene            complement(44159..44842)
                     /gene="phoU"
                     /locus_tag="PLF77_01955"
     CDS             complement(44159..44842)
                     /gene="phoU"
                     /locus_tag="PLF77_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HPC07648.1"
                     /translation="MEERRHTSSHYEMELQDIKNNLIYLGALTEKAIHSAMTSLTDRD
                     TDLAKKVIANDYEIDKMDKDIEEKCIKMLALRQPTAIDLRFITTAIKITGHLERIGDM
                     AVNIAEKAIQLNEEPKLKPYIDLPNMSSIVEKMVAKALDALIKRDLQIADDVRQIEQE
                     VDDLNEQIFRELLTFMMEDSKSIHRALLIMHVSKNLERIADHAQGIADMVTYMITGEN
                     VRHLSRDEK"
     gene            complement(44864..45619)
                     /gene="pstB"
                     /locus_tag="PLF77_01960"
     CDS             complement(44864..45619)
                     /gene="pstB"
                     /locus_tag="PLF77_01960"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HPC07649.1"
                     /translation="MDSRIIQLNNVNFYYGESRALTDISMTFVKNKVTALIGPSGCGK
                     STLLRLLNRMNDLIDGTKVEGEILFESKNIYAPDVDPVEIRRKIGMVFQKPNPFPKTI
                     YNNIAYGPKLTGIAVDDMAALVEQSLKQAVLWDEVKDILNKSAMNLSGGQQQRLCIAR
                     ALAMKPDVLLMDEPTSALDPISTAKIEELIEELKKDYTIIIVTHNMQQAARISDETAF
                     FYLGNLVEYNKTEKIFTNPDVKQTEDYITGRFG"
     gene            complement(45622..46500)
                     /gene="pstA"
                     /locus_tag="PLF77_01965"
     CDS             complement(45622..46500)
                     /gene="pstA"
                     /locus_tag="PLF77_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPC07650.1"
                     /translation="MQILQTHAMKWRYFKQGLFFSCVRLSALMITLALGGIISYILIN
                     GISVISWDFITKPPTDAMTKGGIMPAILGTIYLTLGAIAIGLPLGIASAIYLTEYARQ
                     GRVVRLIKVGINCLAGVPSVVFGLFGLGFFVIFLKFGSSILAGSLTLGFLILPTIIGA
                     SEEALKAVPQTFREASLSLGVSKWQTIYRIVLPNALPGILTGSILGIGRAAGETAPIM
                     FTAAAFFTAKLPSSIFDEVMALPYHIYVLATAGTNIEATRPIQYGTVLVLVAVVLGID
                     LIAIIIRGYMRKNKRW"
     gene            complement(46512..47399)
                     /gene="pstC"
                     /locus_tag="PLF77_01970"
     CDS             complement(46512..47399)
                     /gene="pstC"
                     /locus_tag="PLF77_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HPC07651.1"
                     /translation="MTRQIKEIIIKYIFFIFSLVSVLVLGLIVFSLFREGIPLFGKIS
                     IKDFLFGMEWYPTYDPPSFGIFPLIVGSLIVTILSTFIAVPLGVLAAIYISEIAPQNI
                     KITLKSVIELLAGLPSVVLGFFGMVIVAPWMQETFDLPTGLNIVNASLILALMAIPTI
                     SSISEDALYAVPREFKEASYALGATKFETIARVILPAALSGISTAVMLGMARAIGETM
                     VVLMVAGGAAAIPESLFDSVRPMPASIAAEMGETPFRSLHYQALFAIGIVLFCLTLAF
                     NLIADYVSHKFKQVGSGTL"
     gene            complement(47406..47840)
                     /locus_tag="PLF77_01975"
     CDS             complement(47406..47840)
                     /locus_tag="PLF77_01975"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HPC07652.1"
                     /translation="MQNDSMKKNVLFLCTHNAVRSQIAEAFLNHLYGDRYSAFSAGSD
                     PTQIDPLVVTVMKETGMDLSANQSKSLSVFPDMHFDCVITVCDQVQESCPHFPRGDRH
                     IHKRFSDPSKFNGNAEDITHEYRRVRDEIKAWVEKEFHQAIK"
     gene            complement(47881..48702)
                     /locus_tag="PLF77_01980"
     CDS             complement(47881..48702)
                     /locus_tag="PLF77_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPC07653.1"
                     /translation="MFSILKKAALTIAAVALMSSVALAGSSIVIKGSTTVLPITQSTL
                     EAFMKKHPDVQMSLSGGGSGEGIKALIDKTAHIATSSREIKKEEIALAKTKNVNPVAH
                     VVAHDAIVPVVHPRNKVKNLSVDQLSQIYQGKITNWREVGGDDLKIVVISRDSSSGTF
                     ESWDHFVMKKTKVTPRAQMLASNGAIVTAIAKNRYAIGYLGIGYLDKSVTPLQVNGIT
                     ATIQTALSKQYPFSRELYMYTDGNATGDVAKYLAFVKSAEGQKIVQKEGFVPLTK"
     gene            complement(48815..49519)
                     /locus_tag="PLF77_01985"
     CDS             complement(48815..49519)
                     /locus_tag="PLF77_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC07654.1"
                     /translation="MTMYKILIVDDEKDIVDLVSYNLEKEGFSTVKALDGESALQIIR
                     AQKPDLIILDLMLPKMNGLDVCKTVRRNPETSGLPIIMLTAKSDEVDKVTGLEVGADD
                     YVTKPFSVKELTARVRSVLRRLKENELYAGKEKFSHAGLKINYESCLVLVNGKKVTLS
                     PTELKLLFFLSRHPGRVYSRNQIIDHVWGDDTFITDRAVDVHIRRLRSQIEQDIENPK
                     YILTVRGFGYKFADLK"
     gene            complement(49612..50784)
                     /locus_tag="PLF77_01990"
     CDS             complement(49612..50784)
                     /locus_tag="PLF77_01990"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008369.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HPC07655.1"
                     /translation="MKFEHCIRCTICVENCPVFRVNPEFPGPKQAGPDAQRFRSERDK
                     AQDGWVLLCSQCKRCEMACPCGVEPAQIILKAQQRYGNEHPQTPAHLLFANNYYLSAL
                     GSFTAPLANVVASTQTGKKIFQKMGISTYLPFPKFSFRTMRREKKRLNKGRKKVAFFY
                     GCFINFYRPDIGRKIIRLLASMNVDVVLPPQWCCGLPALGNGNLTLARYFAKKNAESL
                     SNYIDAGYDVIYTCTSCGLCLLHDYPGILEIPQGRKIAENSYDVHEYIIKLINEGYVK
                     PDFREVRRSIAYHIPCHLRALKIGYPAAKLMSLIPGLEYEILDDACCGLSGSYGFKKE
                     NALTAIQIGNRAVSLISKTGMDNIVSDCGSCRMQLAGLSGMPALDPAEIIFDSLGR"
     gene            complement(50774..51982)
                     /locus_tag="PLF77_01995"
     CDS             complement(50774..51982)
                     /locus_tag="PLF77_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit B"
                     /protein_id="HPC07656.1"
                     /translation="MSVTEYDAVIIGAGVAGLTAGTLLAERGLKVALVTKGEPTACLS
                     TGCVDVCSLSKNPLSGIRSLPPEHPYNLVEEKTVKQSLEKFLADMKDAGLPYSGSADA
                     NRLILSALGTSKISCLIPSTMTAAPQENHDGSVHIITFKGLKDFYPGYIVSRRRSTTF
                     SVYDAGVSTTMSIAARFEEKDFFERFIAWMTQRGIHEDGIAFPAVLGMESAADIVDTI
                     SERTGKPVFEIPTIPPSMPGRRLFNALKKTFRNKGGTIYWSWPVAGMEKTGNIVEAVT
                     TPSEGRPNSINARAFILCTGSFVSGGLTATREGILENIFNLPVYVPGPRDTWFDNDYF
                     SLNHNIGKAGIRADASMRPLDAPWKNIFVCGSILADTQILKNGCGHGLALATAHVAAQ
                     SCAEYLLDEI"
     gene            complement(51979..53481)
                     /locus_tag="PLF77_02000"
     CDS             complement(51979..53481)
                     /locus_tag="PLF77_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPC07657.1"
                     /translation="MIKTDVIIIGGGATGCGIARDLALRGIPHCLVEKGDFAAGATGA
                     CHGLLHSGGRYAVTDPVAARECYEENLILRRIGRKCVEQTGGLFVRLPGDSKRFRELF
                     LRGCEDVGIPAEVISSTLAIDLVPNLNPDIEEAIRVPDCSIDPFRLCLLNIRTSELRG
                     GMIFTRHKVTEIIKSHGRCTGVKAVDQTTGEEKEIHAKIIVNATGAWADIVATLAGSE
                     VPLTLVKGSLVITNHRLTNMVINRLRQPSDGDIIVPNEAVCLAGTTSLAIENPDNLLI
                     EASEVDTIISEAGRTIPHFNKTRIIRAFSGVRPLLKSKKADSHEITRGFQIIDHQNGL
                     LSIVGGKLSTFRLMAEKTVDTIMTIFHLKKACETAEIPLDGQEELSGYPLSKRLADMT
                     NIVCECELVTRQNVEKIIQQTATRNVGDISHRTRLGMGPCQGGFCTFRALGIMNDMSI
                     ISPEQSMEMLRGFLQRRFKGIRYALWGDQLREEQLVEYIYLGILSMEKPQ"
     gene            complement(53543..55042)
                     /gene="glpK"
                     /locus_tag="PLF77_02005"
     CDS             complement(53543..55042)
                     /gene="glpK"
                     /locus_tag="PLF77_02005"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HPC07658.1"
                     /translation="MKKSYILSLDQGTTSSRAILYNHKGNIVGTAQKEFTQIYPKAGW
                     VEHDPMEIWGSQSGVVSELLATTAIAAEEIAAIGITNQRETTVVWEKRTGKPVYNAIV
                     WQCRRTADICDELKARGLEKYIRENTGLVLDAYFSGTKVKWILDHVEGARQKAQDGEL
                     LFGTVDTWLIWNLTRGKVHVTDYSNASRTMLFNIKKLKWDQKILKELDIPASMLPDVK
                     PSSMVYGHTDSRLFGAEIAIAGDAGDQQAALFGQACYTPGMVKNTYGTGCFMLMLTGE
                     KLVHSQHGLLTTIAWGINNKVEYALEGSIFVAGAAIQWLRDGMKLIYDAKDSEYFATR
                     VEDSLGVYVVPAFVGLGAPYWDMYARGAILGLTRGTTRNHIIRATLESIAYQTRDVLE
                     LMRNECGLDLCELRVDGGACANNFLMQFQSDILGVPVERPEIIETTAMGAAYLAGLAT
                     GFWKDQSTITRRRNVDRRFNPAMDDDKRHTLYNNWKKAVKRAMKWEEEE"
     gene            55624..>56187
                     /locus_tag="PLF77_02010"
     rRNA            55624..>56187
                     /locus_tag="PLF77_02010"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        14373 a        12931 c        14351 g        14532 t
ORIGIN      
        1 acgttcatga caatttcaga tttgctcttt ttcttcgacg gcattttaaa cgcatgctcc
       61 ttccggacga tagtaatatc aactcgtttt aaaatatcga atgaatatca aattaaaacg
      121 aaagtaattt caagacgata aaagcggaaa taatgagcgc tgtgatccag caaataacaa
      181 tcagcgaacg tcgttcccgc ggatgctcca ggtaatttct gacctccatc tccacccttt
      241 cggcttccag cttatccaca atggattgaa taagtaaagc gtggttttca tacatgttgg
      301 aaaagaaata aaaccctttg gtggtggtta acagaaaata agctttgttc aatatagcaa
      361 caacgcccag atgcgtgaca tccggccacg tcagcgtctt gacacgggaa aattttttta
      421 ccttcatacc ttcattgctt gcggtaatat gtctgttggc tgcttctatg cccaccagca
      481 gggtaaccgc aaaggcaacg gccaaaatga ttttttccca cggttgcccg ttcaacaggt
      541 tcaggatcaa cagcacaaaa agcaggatga taattgcaat aaagggcaaa agaaaagctt
      601 ttttaatttt aaaaacgttc tgctccatgg tttcgcttcc tgtttgattc gtttcagcac
      661 aggctggaca gttgctcggg ttttatttgc ttctgatgaa tgttccgctt ttgccgccgc
      721 ttttttccag cagcatgatg tcgctgatga cgattttttt attcaccgct ttacacatat
      781 cgtaaatggt cagtgcggcg acgcttacgg ccgtcagggc ttccatttcc acgccggttt
      841 ttcccgctat ccggaccgat gcctcgatga aaatcgccga ttttttctca tcaacggtga
      901 acgtcaggtc gacccccgtt aattccagag ggtgacacat cgggataaga tcccccgttt
      961 tttttgcggc catgatgccg gcgatttttg ccgttgtcaa aacattgcct ttggcaatcc
     1021 gccctttgga tattaacgtc agcgttgctg catccatcct gacgattccc cgcgcttttg
     1081 cttcacgcgc ggtctgaggc ttggccgtaa catcgaccat ttgaacattg ccttcatgat
     1141 taatatgtga taattttttt gacattgttg cgaaatattt ttttaacgta attattttgg
     1201 taaagcctta acataggcct gctgatgagt caagaaacgt agcaacattt tttaaatgct
     1261 tgaagttgaa atagcttgat ataaataggc gaatatttta agtgaaacat tcgtgggaaa
     1321 acgggattat cataatcttt agagtattga ccatgaaaat ttcataaaaa acgggatgaa
     1381 gtgtattggc cgcattgcgt gtgcgtgtaa aagattccac cgataaacac aaaatattat
     1441 cattattaaa caaattgcat cccgtgtcct gcgataagcc gttgattcgc ctgggagcct
     1501 tcggtgacgg cggttacctt gtgccggatg atttgacggg aatcgaagcg tgtttttccc
     1561 cgggggtggg tttttcatcc gtatttgaaa aagattgtgc ggacaggggg atgaaagttt
     1621 ttatggcaga cggatttgtg aatcatccaa gtctgtctca tgagcttttt gtttttataa
     1681 aaaaatatgt cggcaatacc gacaatgatc atgaaatgac cctggacgcc tgggtaaatt
     1741 catccctggc tgatgccacg ggagatctct tgctacaaat ggatatcgaa ggtgccgaat
     1801 atgacgtgtt gtcggcggtg tcggaggatt tgttaaaacg gttcaggatc atcgttattg
     1861 aatttcattt gctggacgga ctgcgacgcc aaccattttt taatctggca tcgcaggtgt
     1921 ttgaaaaaat tttacggacg catacttgcg tgcacaatca tccgaataat tgttctgacg
     1981 ccgtgaaagt ggacgacatt gacatcccta tggttatgga actgacgttc ttacggaatg
     2041 atcgtatcca caatccgtcg tttgtcagga cgtttccgca tcctttggat tgtgataata
     2101 cggacagaaa tccctctctt gttctaccga aaagctggta tagaaatcat cagaaatagc
     2161 cggtcgcgtt tcaggcaccg tttcgacaaa acagtgtgaa atcgttgaac aagaaaatct
     2221 cttgatataa atatttctat tggttagtat tcgccccaaa aaatataaaa aggcgttatg
     2281 ttcgttaaag tcaacagcat gagcgtcatc gggatggaat cgtatccggt cttcgtcgag
     2341 gtcgatacat cccagggatt gccgcagttt gcaacggtcg ggctgccgga tgcgtccgtc
     2401 aaagaaagcc gcgaccgcat caaagccgcc atcaaaaatt ccgggtatca ttttccccgc
     2461 agccacgtga ccatcaattt ggctcccgcc gatattaaaa aggaggggac cggttttgat
     2521 ctgccgattg ccgtggggat cctggcggcc gagaaattaa tccgtgaaga aagactgcgc
     2581 gattgtctct ttgtcggaga actttctctg gacggcagca ttaaacgggt gaacggtctt
     2641 ctgtctgttg cgtttaaagc attggaaacc ggcttaacat cggtttttgt tcctgaagaa
     2701 aatgccccgg aagcggcgat ggtgaagggg gtgaacatta tcccggtcaa aacgcttccg
     2761 gatgttgttg aatgtctgga aggacgaaaa accatagagc cgctttgcct cgatttggag
     2821 aaaatttttc acaaaacaaa tcaatacgat gtggatttca gtgaaattaa aggacagaaa
     2881 caggccaaaa gagcactgga gatagcggcg gccggaggcc ataacgtcct gatgctgggg
     2941 cctccgggtt ccggcaagag catgctcgcg aaaaggctgc ccacgatatt gccggatctc
     3001 tcttttgagg aagccatcga cgttaccaaa gtattttccg ttgcgggttt tttggataaa
     3061 gataaaacgc tgatggcggt gcgccctttt cgttcgccgc atcatacgat atctgatgcc
     3121 gggcttgtgg gcggaggaca aatgccgcgt ccgggagaaa ttagccttgc tcatctcggg
     3181 gtgctgtttc tggatgaact gcccgagttc agaaaaaatg ttctggaatc tctccgccag
     3241 ccgctggaag acggcatgat aactattacg cgttcctcgg tgaccgcttc ttttcccgct
     3301 aaattcatgc tggtggcggc gatgaatccc tgtccctgcg gttatttcgg cgaccgcgtc
     3361 cattcctgcc gctgcactcc ccagcagatc cgtcagtatc agtccaaaat atccggtccg
     3421 cttctggacc gaatagattt gcacatcgaa gtgccttcgg tcaaatatcg tgcgctgtcc
     3481 gacaaggaaa cgggcgaatc gtctgcggcg atcaagcagc gcgtgagcaa tgcgcggaat
     3541 atacaaaatg agcgtttcgg cacaaacgga atcatgtgca atgcgcggat gacggaaaag
     3601 cacatccgtc aattctgtca ggtcgatgag gaagcgcaca aactgattga catggccatg
     3661 gaaaaactgg gcttaagcgc ccgcgcggtc aacaggatac tgaaagtgtc ccgcactatt
     3721 gccgatctgg aacatgacga atcgatcaga acgtctcacg ttgccgaagc gattcagtat
     3781 cgaaaccttg atagaggatt gatttagtgc tatgtcaaaa aaagactatc acaaccagag
     3841 cggcgtcctg atgaaatgat tgtccggccg gcagactgga gaaagaggta tttatggaga
     3901 tcaaaataaa aaaagtaccg ctgaaaaaaa gaaaaaccaa gcccgctgat gaatcaaaac
     3961 tcggtttcgg taaaatattc acggaccata tgtttaccat gaaatataga gagggggagg
     4021 gatggtacga tcccgtcatc gaaccgtatc atcctttaca gcttgatccc acggccatgt
     4081 gcctgcatta cggtcaggaa atatttgagg gactgaaggc ctatcgggga caggacagtg
     4141 ccatttatct tttccgtccg atggaaaata ttaagaggat gaatgcatcc gccgaaagac
     4201 tgtgtatggc ccgaatcgat gaagatcttt ttatggaagc catgaaaaaa ctggtgcttc
     4261 tggaaaaaga ctggattcct cacggtgaag gaacgtccat gtatatccgt ccgaccatga
     4321 ttgcgacgga agcggccctg ggcgttcatc cggccaatga gtatttgttc ttcattgtag
     4381 tcggcccggt gggcgcctat tatcctcagg gattttcgcc gacaaaaatg tatgtttcgg
     4441 aggaatatgt tcgcgcggtg cgcggcggta tcggtcatgt caaggccgcc ggcaattatg
     4501 ccgccagtct ttacgccagc agaatcgcaa caaacatggg gtacacgcag gtgctttggc
     4561 tggactccgt ggagaaaaaa tacgtggagg aggtcgggac cagtaacata tttttcgtga
     4621 tcggcgacga attgattacg cctccgcttg gaggatcgat tctacccggt gtgacgcgta
     4681 attcagtcat tacgctggcc agatatctgg ggacaaacgt tgtcgaaaga ccgatatcga
     4741 ttgaagaggt tatcaaaacc tcggaaaacg gttcattaaa agaagcattt gcttccggaa
     4801 cggcggcggt catttcgccg gtcggaaata tttacttccg cgataaggag tatgcgataa
     4861 acggcgggaa aaccggtgcg ttggccgaaa aattatataa tgaaatcctc aaaattcaat
     4921 acggcatggt ggaggacccc ttcggctggc gaattaaaat cgcgaacgga aaaaaacgtt
     4981 cccggtaata attttctgtg gacaaaattt gtaaacgcgc ttagtccaca agactgtgga
     5041 aatcgttgtg gacaactttg ttgataaact ctctaagtgc gtaattgtta cgattttaca
     5101 ccaaattgcc caaggtttga gcaatttaaa tatcaagtaa aatcaatatc ttattaatat
     5161 tccctttagg tatcgccatt taagaagtat ttatgaaaca agatataaac tctgcgaaaa
     5221 aaaatgtgcc agaaaagatc gattttttac aaaaaatgat gaaatttttt cttaatagaa
     5281 ataagctatt tacatcgtca tggattgctc tgagcgttat tgtcgtggca atttccatac
     5341 ctggcagctc tttttccgaa gaacgtccgg ccattgacga tgttgtacaa aaattgcagc
     5401 tgtcctatga gtcaacaaaa gatctcagcg cggattttat tcaggagaca acggtcaagt
     5461 cgatcaaaaa gacacagcgg gaagaaggtc gcgttttctt taaaaatccc caaaatatgt
     5521 catggaatta tacaaagcca caggggaaaa aactggtgat gaatcatcag acggcctggc
     5581 tctatcttcc gcaggaaaaa gttgcctacc gacagagcgc cgaaagcatt tttcaatccc
     5641 gactattgat cgattttttt tccggatcgg gaaaactcaa agatgatttc atcatcacat
     5701 atgacagcgc catatctccg gacaaagacg gtcattatct cctcgtgctt acgccgcgtc
     5761 aaaaaaatac gacgttcaat gtgttgaggc ttacaattga taaaaacaat ttttatatct
     5821 tgcggttaag ttttgatgac gctttcggta attcaacgga tctgagattt tcccgtattc
     5881 aattcaacac cggacttccg aagacgtttt tcctcttcaa gccacccaaa ggcgtacagg
     5941 tttttgaaat gccgtgaaaa cccggggcga tgagcaaatt tccctttgtc gggttaaaca
     6001 ttaaggtttg gcgatagtca cccacaggaa agtttcaccg cctatactgc ggtattgaaa
     6061 tgcgtctatt ttaaacccga tggtttccag gattcccctc agtttttcag gtgtcgtgtg
     6121 gctgtagaac agccgttctc ccataaattt cctgtaaccg ctaaagctgt ccgctccggt
     6181 atacgctttt gtggcgtagg taaaaagtcc cctgccgccc ggtttaagcc agcggtaaat
     6241 tttccggaaa atgccttcgt gcttttcgta ttcaatgtgg aagagacaat aaatggttgt
     6301 taccgcatca aattgttcgc cgggaaactc cacctcccgg atgtctgcgg cgatggtctt
     6361 cattgccggt tggtaacggt gggccaattc cagcattttt gatgaaaagt ccactccggt
     6421 aacctgccat tccttttgaa tgaaataggc gggaaacggt tcacccgcac cgcaacccag
     6481 atcgagcaaa ctgcctttga tggaaggcag tcttctgaag aaatcgtcca tgacatccgt
     6541 catatcgaac aggtaacggt ttttgtcgta agtttgggca aaacgatcgt aaatttcacg
     6601 tatatcaatg tccataaatt cttttcagga tttgaatgga ttatccaggc taaaatccgg
     6661 tgtcaggtcg aagtcgctga cttcttgaat aaacaggtta tgaaaaccca atttctcggc
     6721 atagtccaaa acctgttcgt actctttttt tgtaattcgg cgttttaaat ccggatgatt
     6781 gcggaccttg gccatgggcg tatattgaga catcaggctt aaaaaaacat tgttggatat
     6841 ttttctgctg atcaatctca aaactgcttt ggaattctcg atgcgcccgg gcagaaccag
     6901 atgacgaatg atgatgcctt tccgcgctat cccgtttttt aattccaacg cgtcgccgac
     6961 ctggcgcacc atttctttta tcgagtccgc ggcaaaccac ggatatccgg gggcggcgga
     7021 aaacatcatg ctgtcttcct cctggccgta tttgaaatcc ggcagataaa tgtccaccat
     7081 gccgtccagc cgtttcagga tgtccgggct ttcataacca ccgcaattat acacaaaagg
     7141 aattttcaat cctgccttgc gtgcaatcaa cagcgcctcc atcaccagcg gcatctgtga
     7201 cgtgggcgtg accggttcta cattgtggca ttgccgtttc tccagatcga gcatcacatc
     7261 ggccagttgc cttgaattca tttcctgccc tgtgatgtgg tgggaaatct ggtgattctg
     7321 acaataaatg cagcccaggt tgcaggagga aagaaaaatc gttcccgcgc cgtgcgtgcc
     7381 ggagagcggc ggttcttcgc cgtgatgggc caacgcgcaa tccatgataa tactggccgc
     7441 aagaccgcaa tatccccgtt cgtttttcgt ccggttcaca tgacagtttc gcggacaaag
     7501 gatacagtct ttgaaaattt cctgcaggag gaaaattttt tcttccaaat tcattcttaa
     7561 aaattccgtc cggagcatcc tttggttaaa agggttccac cacatcgcgg agtctgccgc
     7621 tttctaccag ttccagaaag tcgtcgccca gcggagcgga caggtccacc gctttgcacc
     7681 aatttctgat tcttgtttgc gggtcgtcaa tgaatgtgac cagatccgtg cggtcgggga
     7741 cttttccgcc gggaaggctt tccgtaaaat attgagaagg aaagaccatt aaaacattat
     7801 tcagtatatt gggatcgggc gcacggcgcg ttattttttt gtccagccat cccggaatga
     7861 tcctttcctg atgatgaaaa aaaagcacga tgtcgttttc tccggacgca aacggatggg
     7921 tcagatgata atcgatgagg ccgccatcgc ggtacacgcc gcggggagcg ccaaaaatgt
     7981 cgcggacacc ttcaatcacc agcgggattg cgcccgaagc cagcacagcg tatttgaaat
     8041 tgatttcgtt cagcgggaca tagatgccgc ggaattgcgg tttcaggcaa aaagccggcg
     8101 gtttggacgc attgtaaaaa acgactcttt cggaaaattt ataaatattt tctctactta
     8161 aatagttcaa tagaaaacag aaaatcagtc ctgttttctg caataagacg ttttgaaaag
     8221 cgacaaggcc gcgtgaacgc gctgtaatga tggtcagccg gtatttcttg ttgttcaggg
     8281 caaaaggcag ggcgtcattt tccagatacg cattgatgat gccggttatt ttttccagcg
     8341 tcgttgccgg tgtatctttt tttgtgtagg taacatgaat gtaagcctcc atcagttgcc
     8401 ggtaggattt gacggcttcc ggttgcagcc aggcagcaaa acggaaggcg cccgctgaag
     8461 agcccacgag ctggacaggg gtcgaacggc ccagagaaca tctttcaagg agtgtcaaat
     8521 cgaatccgct ggcaatcagc cagcgcggtc caaccgccgg tccgaaatac gtcgaaatca
     8581 gatcaaagtt gaacccgccg tctttgatga gttgataaat tttcctgccg gctttaattc
     8641 gtattctact catagatctt atgtccgggc aaaggccttg acacgtaaac ttttgtcttg
     8701 tagattcact tacagctctt atgaattctt atgtgagaag agggttcata atttctatta
     8761 tgtcttttaa gtggggcacg ttttatcagt aattggtcaa aatgcaagaa aatttatcga
     8821 cgacacaggc cgtgcgtccg gactgtttaa aatggctcaa acagacatgg gaaaatgggg
     8881 caatgtctgc cggacacccg gtgtcatgag atggaagatt tcgcaatggg cagcatcagt
     8941 gatgatctgt ccggaacatc atcatgatga cggcccgacg ttctttactc ggattttttc
     9001 aagtccggcg tgagagtttg gaaaggattc cgaaaaacac tgggcgtaaa aaataccgtt
     9061 tgttattgga caacatttcc ggttccgctt ccggaatccg gaaattcatt tcacgtgagg
     9121 atgattatca tggatataaa agattctgtt gagcacattg cccggaatgc ggcgctggcc
     9181 gcaaaaaaac tttcccgtgt gactgaaacg gaaaaaaaca cggctctttt acgcatggcg
     9241 gaagccttgg aacggaattc taaatttctt ctggatgaaa acgccaaaga tgtaaacggc
     9301 gcgaaaaaag caggcatctc caaagccatg ctggaacgtc tgacccttaa acaatcaacg
     9361 atcgatcaaa tggccaaagg attgagggaa gttgctacgc tgcccgatcc tgtcggtaaa
     9421 atcatttcca tgtggcgccg gcccaatggt ttgctggtgg gaaaaatgcg cattcccctg
     9481 ggagtgatcg gcattattta cgaatcgcgg ccgaacgtta cggtggacgc ttcagcgctc
     9541 tgcctgaaag cgggcaatgc cgtcatttta cgcggcggtt ccgagtcgat caaatccaat
     9601 ctggcgattg catccgtttt gcggaaagta ttacaggata catccattcc cgtggaagcg
     9661 attcaggtca ttccggtgac ggaccgcgaa gcggtggctg ccatgctgaa aatggaagaa
     9721 tacatcgatt taatcattcc ccgcggcggg gaagatctga tccgcgcggt ggttgcccag
     9781 tcaagaatcc ctgtcatcaa gcactataag ggggtctgtc atatttttgt ggatgccagc
     9841 gccgaaatgg ccatggccgt tgatatttgt ttgaacgcaa agacacagcg ccccggcgtg
     9901 tgcaacgcga tggaaacgtt gcttgtgcat cagacgatcg cagggaaatt tcttcccgtg
     9961 gttgccaata aaatgaaaaa agccggcgtc gtcatccggg gatgcgaaaa aacaaaatcg
    10021 attttgaaac gaatcgaaaa ggcggttgaa gatgactggt ctgccgaata tctggacctg
    10081 gttttggctg tgaaggtagt ggaaaacatg aatgaagcca ttgcccatat tgaaaaatac
    10141 ggctctctgc ataccgaatc gatcattacg ggtgattatt ccaatgccca gcgcttttta
    10201 aacgatgtca attcttcgac cgtcctagtg aatgcgtcga cccgtttcag cgacggcttt
    10261 gaactgggac tgggcgccga aatcggaatc tcgacgacaa aacttcatgc tttcggcccc
    10321 atgggcttgg aagaactgac gacaacaaaa tttatcattt acgggaacgg acaggtgaga
    10381 acatgagatg gggacttttc ggcggcactt tcgatccgat tcatttcggc cacttgcgcg
    10441 gggcggagga aatgctcgaa attttcaatc tgaatcgtat catctttgtt ccatcctcca
    10501 gaccgccgca caagctggaa gcggatatca cggcgttcaa tcatcgcgag cagatgattc
    10561 gtttggctat tgaagataat gtcaatttct ctttttccga ggtggaaaat ctgcgcgccg
    10621 gtaaatccta ttccgtggaa acagtcgaat acatacttaa taaatacatg gaagatctgg
    10681 aactttattt tattgtcggg caggatgcgt ttcaggcggt gacaacctgg aaggattggg
    10741 aaaggctttt gctattatgt aattttgccg tgatgacccg tcccggttat gatgatatga
    10801 ggcttgatgc aattctgccg agaacctttg ccgcaaaatt catctacgat aaaaaaaccg
    10861 acggatttaa aggtccaacg ggacaggcca tctattttcg ccatacgagt ttcctggata
    10921 tttcatcgtc gcacatgaga gacatggtca gaaccggcaa ttccatccga tatcttacac
    10981 ccggcaaggt ccgtcaatac atcatgaagc ataatttgta caaggagtgc cgggacaatt
    11041 gaacgtgtaa aattttttta aggagaaaaa tatgaattat aaatttattg aactggaggt
    11101 caaggatcat atcgcaaccg taaccctggc cagagaaaaa agcttaaatg ccgttagcct
    11161 ggaacttgcc tcggaaatta cggatgtgtt tagaaaatta agtgccatgg atgacgtcag
    11221 agttgtcatt ttgaaaagca gggccagaat tttttgtgcc ggtctggatt tgaaggatgc
    11281 cgccggtggt tttacgggaa caaccagagc gccatgggat atgattaaaa aatcacagcc
    11341 gttgtttgat tgctgcaatg tcattgaaga atgtcccaaa ccggtcattg ccgcggtgca
    11401 tggtcagtgc attggagccg ggcttcacat tatttccgcc tgcgatatcc gcctctgtac
    11461 tcaggatgcg acgttctgcc tgaaggaacc gaaaatcgga ttgtgcgccg acatgggcgc
    11521 gcttcaacga ttgcctctga tcgtggggca gggctatgcc agagaaatcg cgttcaccgt
    11581 tgccttttat aatgcccagc aggttgagaa aatgggactg gtcaatcatg tctatgctga
    11641 tcaggcggcg ctttatgagg cggcccgtga aatggctcga caaatcacag agaacgctcc
    11701 gctggctatt gaatgcacga aggaggttct taatttcagc agaggtacga gcgtcagcga
    11761 aggaatgtct ctggccattc aaaaaaacat gttcctgctc gccacagatg acctgaaaga
    11821 agcctttgtt gctttcatgg aaaaaagaca gccggatttc aagggcagat aaagagtcac
    11881 ccccattgac ataatgacca aaaaataggg ggacggtcca cgatgaccgt ccccctatgt
    11941 ctttttttaa gacttccgcg atgttaatct ttgtacatgc tttcaatatc gtccttgaac
    12001 ttttcaaaaa cgattctgcg ttttaacttc agcgtgggag tcaattcgcc cgtttcgacg
    12061 ctgaagggct tatcgagtag tttgtaatat ttgatctgct cgtagcgggg cagtgcttcg
    12121 ttgacttcat caacctgttt ctgaatcagt tctttgacct gttcgttggc aagcaggtct
    12181 ttatcggaac acgccgggat gccgttgtgt ttggaaaaac gatgcagttc ctgaaaatcg
    12241 ggaacgacca gtgcggaaat gtattttctc gcatctccga tcaaacagac ctgatcgata
    12301 tacggtttgg cggtaagggc cagttcgatc ggatgaggtg caatattctt gcctcctgat
    12361 gtgacaaaca gttccttttt gcggtcggta attgtaagaa atccgtcctt gtcaaattct
    12421 ccgatgtcgc ccgttttgaa ccatccgtca tccgtgaaag cttccttggt tgcgtccggc
    12481 agattccaat aaccttggaa gacctgaggt cccttgacaa gaatttcgcc gtcctctgcg
    12541 attttttctt ccgtccacaa cgccggtctt ccgacggtgc cgggtttaat ctgcaatttg
    12601 tgggcgagcg tgttgtatgc cagagaaaga ctcaaggagc ggagaggatt ggtataggga
    12661 gaaagccctt tggcctgctt ggtgaccatc aaatcaaccg ttaaatcaag catctttttt
    12721 tgaaaccaac tggccttgct gatatcaaag ctggtggcga attccggttc gttgaaattg
    12781 atgacgggcg acgtttcggt cagtccgtaa ccttcggcca gctgaatgcc cagagcccgg
    12841 ataaaaacgc aaacttcttt ggatagcttg ccgccacccg atatgccgag aacaacatga
    12901 tccaatccgg ctgttttttt gattttattg aaaaccagaa tgttcgccag cgcgaactgg
    12961 agcagatcca agaaacccag tctggtcgct gtagccatcg cttcgcagta tttttcaccc
    13021 agccgggtgg cccagttgaa aatggattgg acccgtttat cctgacggga cacaatcgac
    13081 cgggtcgtat cataaatctt ttcccataat cggggaatgc tgattaaaac attcggtctg
    13141 acttcgagaa tattctttgg gattttttca aagctttcag caaaaaccag aatcccgctt
    13201 tgctgcagag cacccacatg ataatcgctt gtccggccga atacgtgaca catcggcagg
    13261 tgaaccagga aagtcaggtg aagtcccagt tcacgctgtt tccgcatcag cgtggaattg
    13321 gaagcctgaa gcatgttgga tacccagttt ttctgggtca gaatggcgcc tttctgtttt
    13381 cccgttgtgc ccgatgtata aatgatggaa gtgacgtctt ccggagtgac gctgttgata
    13441 tttttttcta tttcttcgtc gctgattttc cctttgccta agtcgataac ttcatcaaac
    13501 gtgtaaaccc cttcgggttt tgcgccttcc caggagcaca tgacgatgat tttttcaatg
    13561 ggaaccccct gtgcttttgc tttgagagcc atctctccgc ggccctgtgt ggacgcaatg
    13621 acaattttgg atccgctgtc cgatgtcatg tagcccagct cttccacggt taacgtcgga
    13681 taaaggggaa cgccgatggc gcaacaggcc tgaatggcct gatcggcaat aatccatctg
    13741 ggtctgcttt ccgaaaaaat aacgatacgg tcgctttttt ttagccccag gaccatgagc
    13801 cccttggcga ggccgatggc ttcctcccgg gtctctttcc atgtccggga atgccattga
    13861 gcgctgggat tgccgttttg atcaaacttg gcccgaagca gttcaacctg atcaccgtaa
    13921 cgcaatgcct gattgtgaaa aatgccgcat aaagtatccg tcctgtatgc cataactaca
    13981 tcctcccccc tgtaaagtat ttaatgaact atcgtgatgg tgttggtaga tagttttctt
    14041 gtttattaat ttttgatttg cagtgcgttt tcatacgctt ttttttagtc aaaaatcaag
    14101 tttaaatttt cggatcttca ggaatatcga aggcggcgtt tttcttttta taaagaaaac
    14161 gctctaaaaa acgatgatac acggtccaat cggaatcggg actggaggac tcgttcatga
    14221 aggtctgaaa agcattgaat ttggcgtcca gatcgtcgat ataatggacg acgagcgctt
    14281 ccagagtctt cggacgcttg ggagaaccga attcgaattc gccgtggtgg ctgagaatga
    14341 tgtgtcgcat ttccattgcc agctgcggcg gaaaaccgtt gattgccgcg attttctgat
    14401 cgatcatttc cacacccata acgatgtggc cgatcaatct tccttcgtcg ctgtagtcaa
    14461 taatgctctc gaaagagaat tcgtaaattt tgccgatgtc atgaagaatg cctccggtga
    14521 tcagcatatc cctgtcgagg atggaataat gatcggcaac tttatccagc aggcggacca
    14581 ccgaaagcgt atgttccaga agtcctccca ggtaggtatg gtggaatcct ttggccgcgg
    14641 gcgctcgccg aaaacgggct gcagtttcct cttcctgcag aaaagcattc agcaatgcct
    14701 tgagatgcct atggtttact ttttccgcaa aggccagcag ttccgcaaac attgttttga
    14761 cgtcggtctt taccgcaggc agataatcgg caggatcaat atcttgccgg ggaatgttct
    14821 tgatgttgag aatggatatt tgcagcgagt ttttgtagct tgcggcccgt ccttcgataa
    14881 aaatgacatc gccttttttg aagacgttat cgaattccaa ggcgttttcc catactttac
    14941 attccatttc gccggtttta tctttcagcc ttaccgtcag atagggggag cctttttgtg
    15001 aaaaggccat gtttttttcc gttaccagaa aagaagaagc aactttatcg ccctgtttga
    15061 tatccttgag ataaacgttt ttatggttca aatgatgacc cgcctttcgt gagagtatat
    15121 gctcatccgt cctttcctga catagccgat cagcgttata ttggcctgtt ttgccagggt
    15181 cacggcattg gccgttggtg cggaatgaga gatgatgaac ggtacgccca tgttccatat
    15241 cttatagact atttccgaag aaattctgcc cgtggtcaat atgatggccc gatggagatt
    15301 gagtttattc agcagagcat atccgcccag catgtcgatg gtattgtgcc ggccgatatc
    15361 ttcgcgcaga gccgcaattt tttttctaaa ggccagggcc gagcagtgcg tgccgccagt
    15421 ttcattatgc atcgccgaac cgtttaaaag atcgtccata agtttcagtg ccagcgctgt
    15481 gttgatactc aagccggccg ggacgttcag cggcgtcagc agagcgacgt tgcttcggcc
    15541 gcgtgcgccg ctcgatgcaa tatttttcac ggacacgtcc ggaaaaaaat ttttgttctt
    15601 caaaagaacg tcaacccggt ttttggcaag ggagatgttt tcaatgtcct gccggcgggt
    15661 gatgatcttt tccgatcgta agaatccggc ggcaagttct tcgagatgaa tgccggcgca
    15721 ggcaatattg gccagggggg ttttgttgac ccggacggtg atcatcactt cctggatcac
    15781 tttgacgcgg gatccggaca cggcagaata gctgccggaa aagcatctta tcgtcttgat
    15841 gattttttgc aaatcgttca tgttttttcc cttaatacgc ggtgatctcc tacccgaacc
    15901 gtcagtttat tcatgtcaaa gtattccatt agtggaatga tatatttgcg ggaaagacct
    15961 gtcagttcct taaaactggc cggtgttgcc ttgccgtctt taatcagcat tgccttataa
    16021 tcattgcgca gtttgctcag gatttcgccg gaaaagcata gttcttcatt gatcttgatc
    16081 aattctcctt ctttgagcat caggctgaca atgttttgtg ctttgttttt cctgtcaata
    16141 aatttgttga tgacgtctgt cagcgaagga ggcatcagtc ctgctgtttt gtatattccg
    16201 ataatattct gacgcagagc atccagatca cccgcaagct ccacctgatg tttggccaga
    16261 cgcacattat ctttgttgct gacgatcgtc tcttttttgt tgagcgtgtt gagaaccatg
    16321 ttaaagagtt tcggtgaaat atcattggca agggatccct tcagctcttc tttgggaatg
    16381 ccttctttca ggggattttt cttgtgatac ccactgagat tataaatgat catctcttcg
    16441 agtcgattga aaaaacgtgc ggaaataacc gtcgtgtcct cgctatcaag cagaaacgcc
    16501 tcctttttgg aaaataaatc gtccagaacc tctcgtattt ttctggattt cattcccaga
    16561 cggaaggcca attgttgaag cggggttcct ttaaacccgg tcctgtccag aatgacggta
    16621 attttttccg gtaaggtgcc tttttgcagc aacatcagtt cttcgatcgc tccgcggttg
    16681 aaccgtttat gtttttgcgg aagcggatcg ataatgacgc cgccgccgat ggtggtaacc
    16741 ggggaataac tgcgcaagac aaaatgatcg ccggcgacaa caacgtcttt ttcagaaaga
    16801 atgagttggg cgtatccttg ctgcccgggg gcaagttcat ctgtatccag aagaataatc
    16861 cgggaaatga tttcgttcgt gccggtgtgc agccgaacca gcgcgcggtt tttaagactt
    16921 ttggtattgg cggaaagata gtcgacggcg atatccagcc gctgcgtagg ccagacggtg
    16981 tgcggccgca ccagaacatt gcctcgcacg atggcggatt tttcaatacc ttgcaggttg
    17041 accgctgtac gctgtccggc ccaggcttct tcctcaggcc ggttatgtac ctgaatgcct
    17101 ctgatgcggg cggaaatatt ctggggaaga atctgtacgt cttcccctgt tttaaggtga
    17161 tcggaaacca gggtaccggt gacgattgtg ccgaaaccct tcatggtaaa aatgcgatcc
    17221 acgggcagac ggaaaatacc gtcgtccgtt cttgcggtaa tctctgcgat gatggtatcc
    17281 agggcctgaa gtagctcgga caacccctcc tgttttatgg cggaaacggg aatgaccggt
    17341 gcgctttcca gaaatgttcc tcgaagaaaa tcggtgattt cagactcgac aagattaagc
    17401 cagtcttttt ccacgagatc aatcttggtc agggctacga ttcccctgga gattcccaga
    17461 agggaacaga tgtgcagatg ttctcttgtt tgaggcatga tgccttcatc tgcggcaatg
    17521 accattaaaa ccagatcaat gccggcagcg ccggaaacca tgtgtttcac aaacttttcg
    17581 tgtccgggaa catccactat cccgagtgtc agacccgaag gcaatttcag ggaagcaaaa
    17641 ccgagttcga tggtgatgcc cctttgtttt tcctctttga gacggtctgt gtcaattcct
    17701 gtaagagctt tgattaaagc cgttttcccg tgatcgacat gaccggcggt gcccaaaaca
    17761 aaatgcttca ttttttaatc ctgattatct gaaagtgaaa aataacaaac tcttcatttt
    17821 ttaacaaggt tatatttcaa gacatcaaaa ataaaaaacc catgttgaaa aaaactggac
    17881 aaattaattc ttgaaaatgc cggatggaat tgttagaaaa aaataaagag gtattttttg
    17941 cgtatacttg gtcttgatta cggcgaaaaa cgaatagggg cggccatctg tgatgaactg
    18001 gggttgacag cccagggatt gtccacaatc atacgaaaaa caaaaaagca tgacggggaa
    18061 attttagaga ggctcatcag ggattttaac gttgagaaaa tcgtcatcgg gtatcccctg
    18121 aagctggacg gttcggaagg catccagtgc gaaaaagtta atcgttttgc cgcgctgctg
    18181 gaaaaaacct tcggtttgcc ggtgatcagg tggccggaaa cgctgtccac caaagaagcc
    18241 gaagaaatac tgatcaactc cggtatccgg tggggaaaaa gaaaaaaaat ggtcgataaa
    18301 ctggcggcct gtcttattct tcaaagttat ctggattctg tcgccccatc attaaaaaat
    18361 aatttctgaa caaccatgaa aaatcctgat aaaaagaata tcctgaaaaa gtcaaatgaa
    18421 gaaaacaacg cggtcgatca aatgacgtca caggcaagtg cttggaaaag taaattccat
    18481 gaaatcttca tttttctgaa aaaaatattt tccggaagaa gatggattcg ccgcactgtt
    18541 ttatacagtg ttgccgtcat cctcatattc tgtattatgt tattggatta tgcattcagt
    18601 ccgatcgatg tccgagataa aacggttgtg attgatatcc cgacaggttc cagcttcatg
    18661 gaagcgactg aattgcttca gaaagaagga ttaatcacaa accgtttttt cttttacagc
    18721 ctggccgcga tcaagagggc cagtcgtaac attcgtgccg gagagtatga aatcaatacg
    18781 ttgacgaacc ccataacaat gattaacaaa ctcatgcacg gtgatataaa agtctatgag
    18841 gttgtgattc cggaagattt gtcatttcgc gacatcgcgg tgattctgga caagcataag
    18901 ctgattaata aagaaatatt ttttgagctg gccagggatc aggaattctt aaaatcgttg
    18961 aatattgaag cggagtctat cgaagggtat ctgtttcctg atacgtatca ttttaaccgt
    19021 tcgatgaata cacgtcgcat catgaagaaa atggtcgata cattctggca gaaaatgacg
    19081 ccgtccatga ttcggagggc gaatcagatg ggattaagcg tgcataaatt agtgaccatg
    19141 gcctccatca tcggcaaaga gtccggtagt gctctcgaga aaccttccgt tgccgctgta
    19201 ttttacaaca ggttgaaaag aggaatgcct ttgcaaagcg atccgacggc cgtttatgac
    19261 atggatgact ttgagggtaa aatttttcgt agtcacctga agaggaattc tccatataat
    19321 acctatcgga tcaaagggtt acctccaggg ccgattgcca atcccggctt ggattcgcta
    19381 aaggcaacgc ttttcccgag ctctgtcgat tatctttatt ttgtttccaa aaaagacggc
    19441 actcaccatt tttccagttc attggtggaa cataatcagg cggttaatcg gtatattaat
    19501 ttaaaagatg aacaaaatca attacttgaa tatgatattc ccgttgagga gtattaaaat
    19561 aaaaaatcca gcctttgttt aaaaatttcc tgtttttttg tttttctcgc cataaaatat
    19621 ttttttatca gaatttccct atttcttcag taattttaag ctatttaatt taaaaaaaag
    19681 ttgcattttt tacttgacaa agacaaatag cgcctctata gttgccacgt ggagtaaagt
    19741 ggcttatcgt ggagggaagt gatgtctgtt ttcagggggc agtatcacca tacaattgat
    19801 gaaaagggaa ggataatctt tccgtcaaag tttcgtgaaa tctttgctga agagtatgac
    19861 aaccggatgg tgattaccaa ctgggatcag tatctcatgg tctttcctta cgatgaatgg
    19921 ctggttattg aagagaaggt cgcccatcaa tccaagttgc gcaaggaagt cagatcgttt
    19981 cagcgtttct ttatgtccgg agcgattgat tgcagtattg acggacaggg gcgtgtgctg
    20041 attccgccgt ctttgcgcga atatgctcaa ttggaaaaag atattgttct ggccggcatg
    20101 ctgaaaaata ttgaaatctg gtcgaaggac cgtttcgaag aaaggatgaa gcaggctgtc
    20161 ggagaaattg acaaatgcgc cgactttata gctgatctgg gaatttaaac catgacggcc
    20221 gagttttatc accagccggt catggtgaag gaagttatag attcgcttat cgtcagcagg
    20281 gccggggttt atgtggatgg caccgttgga ggggctggcc attcatacgc gatcctcaag
    20341 cagacggatg ctttgctgat cggcattgat tgcgatgatg aagctctgcg gtttgccgaa
    20401 aaaagactgc aagaattcgg ctcacgcaaa attttactaa aagaaaattt tgttaactta
    20461 gaccatatac tgaaaagttt aaacatagaa aaagttgacg gtgtgcttct ggatctgggt
    20521 gtttcatccc gtcaactgga tgcatctgaa cgaggtttca gcttcagtca gcaggcgccg
    20581 ctcgatatgc gcatggatcg cagcatgacg ttgagcgctt atgatattgt caacagcttc
    20641 gcgcaaaacg aactggaaaa aattattagg ttttacggag aagaaaaaat ggcttcaaga
    20701 atagccaggg caatatcaaa aaaaagacag gaatcgccga ttgcaacaac gacacagctg
    20761 gcggcgattg tcgcatcctg catgcccgcc ggaacgcgat ggcgcaaaat tcatcccgcg
    20821 accagaacgt tccaggcgat cagaattgcc gtgaatcacg agttggacaa catcaaaccg
    20881 gccatgcatg cggcagttga tgtgttaaag accggcggcc gtctttgcat gatctctttt
    20941 cattctctgg aagaccgtat tgtcaaaaat gagattcgtt cgctggaagg gcgttgcgta
    21001 tgcccccccg acagtccgtt ttgtgtctgt cagagagatg ccaaacttaa aaatctgaca
    21061 aaaaaagcga tcatgccttc ggaggaagaa atagcgtcca atccgcgggc gagaagcgct
    21121 aaattaagag tagccgggag attgtaagat ggccccatcc gctcaatcag cagacgcacg
    21181 agttgtcccc catctgggag aagtcgaagg agcgtccgtc ggaattaaat atccgacgtt
    21241 tattgttctc gccgttgtgc tgatgcttgt gtttgttgct tatgtcggct cgcatattcg
    21301 catgaccgaa ctggaataca atatcgcagc ggaaatcagt acccaggaga aaattctgga
    21361 agaacagaaa aaattgaaac tggaatatgc catgctcaaa tcgccgcaac gaatagaaaa
    21421 tctcgtccgc gacagattgc agatgtccta ccctgacaat gaacaggtca ttgttataag
    21481 aaaatcggga ggctgacatg gcgattgaat cgaaaaaaag aatgaaattc agaatcatca
    21541 tgattttcat gttttttttc gttttatttg tggcgctcat atcgcgcgcg tttcagcttc
    21601 aggttttatc cggtcaaaag ctgaagaaac tggcaacaag gcagcatacg acggttttgc
    21661 agttgcagcc ggaacgggga attatttacg accgcaacgg tgaaaaattg gccatttcca
    21721 ttatggccga ttccgtctgt gccgatccgt cgaaagtcat tgatccggcc aaggcatcca
    21781 aacagattgc gtacatcctc gggttggaca gtcaggcggt gtataaaaaa ttatccgccc
    21841 ccagaaactt ctgctggctg gccaggcgga tttcgcctga gcaaaccgct gcagtggtga
    21901 aagcggatgt cgagggcgtt tttctggtca aggagccgaa acggttttat cccaatggag
    21961 agttggccgc ccatctgctg ggttttgtcg gattggatgc ttccggtctg gaagggttgg
    22021 aaaagaaata caatgactct ttagtgggca gcccggaaaa acttgaatgg gcgagggatg
    22081 ccaaagggaa gaaacttttc ctccgggtgg aaaaaacgca gaccgcaaaa aatgaaaata
    22141 ccaaccttgt cctcacgatc gacaaccgaa tccaatatct tgttgagacc catttaaaag
    22201 aggcggtgat tgaaaaaggc gccaagggag ggctggctat tgtcatggat ccgaagacgg
    22261 gagagattct ggcgctggcc aatcagatcg ggtttaatcc gaacaacatc cagggactgt
    22321 ctcccgagcg atggcggaat cgcgccatta ccgattatta tgatccgggg tccacgttta
    22381 aaccgttttt ggtggccgcc gctctggaag aaaaagttat tcgcgaacag gacagatttt
    22441 tttgtgaaaa cggcaattac aggattgccg atcgcgtgat tcatgaggcc aacagaaaac
    22501 gttacggcac attaacggtc agggagatct taaaatattc cagtaacatc ggttccgcaa
    22561 agattgccca gaaaatgggc aaagaaaaat tttataatta tattcaaaaa ttcggtttcg
    22621 gatccaaaac cggaatcgat ttgccggggg aatcttccgg actggtgcgg ccggtgaggg
    22681 actgggtaaa agttgataca tccatgattg ctttcggtca gggtatttcc gttacggcca
    22741 ttcagttgat cagtgccgtc tccgcaatcg cgaacaacgg cgttttgatg aaaccgtata
    22801 tcgtgcgcgg cattgccgac caggataacc ggttggtcaa actctataca cccaccgtgg
    22861 tgcgtcaggt catttctccc agagtggcca agagcgtgac gaatatgctg atggatgtcg
    22921 ttggtgccga agacggcacc ggtaaaaatg cgaagattgt caatgtggcg gtagccggca
    22981 aaacaggaac atcccagaaa ttcgacaccg cccgcggcgt ctactcatcg gaaaaagttc
    23041 gtacgtcgtt tatgggattt ttcccggcta aagatcctca ggttgccata ctggtggttc
    23101 tcgatgagcc gcaaaaagac aaatggggtg gcgttgcgtc ggcgcccgta ttcaaaaata
    23161 tagcggaaca gattctgaac tgttttaaaa ccgatatcag agaaaccccc gtttttaaga
    23221 aggaaaaaac cgataaggtg cagttggtgt cggcggaaca aaccttgctc cctgaaattg
    23281 tcgaaaacag cgacggaccg tccatgccca attttatggg attaacgatc agggaagcgt
    23341 tgaaaaaagc aaaaaccgaa tccatcgagc tgaaaatatc gggcaacggc tgggccgtgc
    23401 gtcagtaccc cgaaccgcac gcgcctctgg gccatgaacg tgtatgcagc gtggtttttg
    23461 aattgacgga ttgaggatgg catgaaactg actcggttga cggaaggatt ggatatgatc
    23521 aatatgtcgg gtgaggcgtc cggagacgtg tccggtgttt gtttttctgc ggaccagtgc
    23581 gtgccggagt ctctgtttgt tgccattccg ggattaaaac gtgacgggca cgatttcatt
    23641 gaggaagcca tatcccgcgg cgcgagattc attgttcatg agaaggttct gacgttcccg
    23701 ccattcgtaa gagcgatcaa ggtgtccgac agccgccggg cgctggggat cctggccaag
    23761 aattatttcg gtgatccttc cgcggcgctg accgtaattg cggtgatggg caccaacggc
    23821 aaaacaacca tctcctatct gctggaatcc atcttaaaag gagccggatg caaatgcggt
    23881 gtcctgggca ccgtcaatta tcgctataac gacaaggtct atgatgctcc caacactacg
    23941 ccggaatctt acgagatgca gaagatcctc cgcgccatgg ccgacgacgg cgtcacccat
    24001 gttgtagccg aggtttcatc gcatgccgtt gatttgaaga gggtggacga ttgtgatttt
    24061 gatctgggta tttttaccaa tttgacccgt gaccatctgg attatcacgt cacgatggaa
    24121 aattattttc aggcgaagaa aagatttttt gcggaaatcc ttccgcaaag cagaaaaggt
    24181 tatgcgcaga aaatgatcat caacgccgat gacgactggg gacggagaat tctgaaggac
    24241 gtgcccctgc cggcactgac ttacggaata gaaaacgact gtgccgtacg atcgttaaac
    24301 tgcaagctgt ctctgaacgg catagaagcg gacatccgct tggcgggaaa caatgtcttg
    24361 atccaatcac ccctcatcgg caaattcaat ctgtacaata ttctagccgc tgccggagcg
    24421 gcttttattt tacacattcc cgtgccggtg attaaagcgg ggatcgaaaa tttatcgcat
    24481 gttcctggga gattggaatc ggtgccttcc gccggagggg ttcacgtttt tgtcgattat
    24541 gcccatacgg atgatgcgct gcggcgtgtt cttcaaaatt tatccgagct taaaaaaaac
    24601 aaaatcataa ccgtttttgg ttgcggcggc aacagggaca aaggcaaaag ggccctgatg
    24661 ggagacgcgg ccacccggta cagcgatttg acgatcatta cctcggacaa tccgcgtttg
    24721 gaaaatccgc tggatatcat cgctgatata gaagcgggca ttgaccggaa agacgttaaa
    24781 aaaagcgatc cggaaatgtt aaaaatgaac ggacgtgctc gttcatatgc agtgatcccc
    24841 gaacggagaa aggcgattga ggcggcgatc agggccgccc ggagagacga tatcgttttg
    24901 attgcaggca aaggccatga agattatcag attctcggga cggagaagat atcgtttgat
    24961 gaccgggttg ttgcacggca gattcttgaa accaagagtt attgagacga acaatgaaga
    25021 gtgagtcacc tgtatttttt ttgaatgacg tgctgacatc aacgtccggg acgatgatgt
    25081 ccggattgcc ggaaactgtt tttcgcggcg tttccacaga ttcccgttcg ataaaaaaag
    25141 gcaatctttt tgtcgccctg aagggggatc attttgacgg tcacgattat gccgagtcgg
    25201 tttttcaggg aggcgctgcc ggaatactgg cgcaggatga tgaaaaaata cgccggatga
    25261 aagtggatga tacctttgcg gtaataaagg ttgccgatac attattcgcg ctgggagaga
    25321 tggcgcatga ctggagaaag aaattttcca ttccggtgat cggattgacc ggatcttcgg
    25381 gaaagacgac ggccaaggaa atgatagcgt ccatcctcgg gttgaaaaaa aatatcctga
    25441 agacggaagg gaatctgaac aacctgatcg gattgccgca gacgatattc cgcctgacgg
    25501 atcagcatga catgatggtt ctcgagatgg ggacgaatac ccggggagaa attagacgac
    25561 tgacccgcat tgccgaaccg gatatcggat tgattacaaa cgtgggaccg gctcatctgg
    25621 aagggtttgg gtccctgcag gttgtcgggg aagagaaagg ggatttattt ctgaatatgc
    25681 catcgtcggg aatcattgtg gtcaatctgg atgatgaagc catcctgacg gctgctgaga
    25741 cgtggaaagg caggaagatc acgttcagca tgagccccaa tgcggatgtg accgtctttg
    25801 atatcgaaag aaacggtgcg aaaggagttc gtttcaacct tgctatccgg ggcaatacgc
    25861 agaaagtgga aatgaaaata accggacttc atcacatcaa taacgcgatg gccgctgcgg
    25921 cggtggcagt ggcagcgggg atggatctga aaaatattgc ggaaggtctc tcggcgttcc
    25981 gtccgtttca cggacggatg gaaattatca aactccgcaa cggcgcgtat cttctggatg
    26041 attcctacaa tgccaatccc gcatcggtcc gggaagccct gatgaccctg aaagatttaa
    26101 agggcaatca ccatgcgtac gtttttctgg gagacatgct ggaactgggc gcacaagccg
    26161 atgaaatgca ccggaagata ggaatgctga ttgccaccat cggtgtgaag acgctgtttc
    26221 tccagggtga ctatgccgga atgaccgctg ccggcgcgac ggaaggaggc atggcgtccg
    26281 acagcatcct ctttttgtcc gataaggaaa acggtactga ttttctgaaa aaatgcctga
    26341 aaaaagggga ctggattctg gtgaaaggtt cgcgccgcat gaacatggac agaattgcgg
    26401 cgcagatctg tgaagatttc ggcagagata aagtcagcga ggagaacaag acagactatt
    26461 aatcaggagt aaagaagatt gatttaccat ttactgtatc cgctgcatac caccatttcg
    26521 ttctttaacg tttttcgtta tatcacgttc aggacgatct ttgccgccat tacggcgctg
    26581 ctgatttgtc tgatttttgg gaaatggatg attcggaagc ttcaggacat gcagatcggt
    26641 cagcagatca gggatgacgg gccgcaaagt catctggtga agaaaggcac tcccaccatg
    26701 ggaggcatgt taattattat ggcggtcgtc gtatccacgt tgctctggac gaatctggcg
    26761 gacgtgtatg tctggctgat actcatggtg acgcttggct tcggtttgat cggttttatc
    26821 gacgattaca gcaaactggc cggcaagagt tcccggggag tctccggaaa aacgagactg
    26881 gcggccgaga ttatcattgc tctgtttgtc agtgtgattc tttatgtgaa gccaggtttt
    26941 aacgcgcagg taacgattcc ttttttcaaa acggtattgc ctgatcttgg ttggggctat
    27001 gttctgcttt cgacctttat tattgtcggt gcggccaatg ccgtcaattt gacggatggc
    27061 ctggacggtc tggccatcgg tccggtcgtg atttgttttg ccacctacat tctttttgcg
    27121 tattttgccg gaaatataaa gacatcgaat tatctgcaga ttgttcatgt ttccggagca
    27181 ggagaactgg cggtatttgc cggggcgatt gtcggtgcgg gactcggatt tttgtggttt
    27241 aacgcttatc ccgcggaagt attcatgggc gatgtcggat ctttgtctct gggtggcgcg
    27301 ttgggcacga tagcggtcat taccaaacag gaaatcctgc tggccattgt cggcgggata
    27361 tttgttgtgg aaaccttctc ggtcattttt caggtcggtt atttcaagat gaccgggggg
    27421 aaacggattt taaaaatggc gcctctgcat catcacttcg agttgaaggg ttgggcggag
    27481 ccgaaagtca ttgtcaggtt ctggatcatt tccattttgc tggcgttaat ggccatcagc
    27541 acattgaaat tgcgttagag ggacaatgga ttttaacggt aaaaaaattg ttgtcatcgg
    27601 cgtgggaaaa acaggcgtgg ccgtcgcccg gtttctgggg aaaaagagcg ccagaatcgt
    27661 ggtgactgat gaaaaatcac cggaccattg ggatggcgga tttaagcaaa ttgccggcga
    27721 gccatggctg gaagtcggcg aatataacag cgtggatgtt ttaaaagatg cggatatggt
    27781 tattccttct cccggcattc ctcccggtaa tcctttgctg gtggaggcct tgaagaagaa
    27841 cattgccgtc gtcagtgaaa ttgaactggc ctaccggttt atcagggttc cgattatcgc
    27901 cgtaacgggc accaacggta aaacgactac taccaccttg ctgggtgaaa ttttgcagaa
    27961 atcggggaag aaagtttttg tcggcggaaa tatcggcaat ccgttgattg aatacgcaga
    28021 taaaaatcag gatgccgatt ttatcgtggc tgaaatcagc agttttcaac tgcagtggat
    28081 cgatcaattc cggcccttca ttgccgttct tttgaatatc accagcgatc atgtcaatta
    28141 tcacgggtct tttgccgagt atcgccgcat caaagccaga atctttgcca atcagaccgg
    28201 aaaagatttc gcgattctga atgccgaaga cgatgaacaa aagaagatcg aagggaacat
    28261 cagagcgcgt gttgccggat tcagttcaaa gcatgttctg ccctcgggaa tatttgttaa
    28321 agaagagaat attgttttga gtctgccggg gatgcaagaa gaaaaatatc ctttgagcat
    28381 gatcaacatt cccggcttgc acaatgtgga gaatgtgatg gcggcgattt tagcggctcg
    28441 tttctgcgga tgtgatccgt cggatacgat ccgggctgtt gaaaacttcc gggggttgcc
    28501 tcaccgcatc gaatttgtgg aagaaaagaa ttccgtcaag ttcttcgatg attccaaggg
    28561 cacgaatgtc ggatcggtcc tgcgcgcgct ggaaacgttc acccagccgg tgattttgct
    28621 gctgggcgga cgggacaagg acggggattt cgaaaccctg cagccggtgc tggcggccaa
    28681 agccaaaaaa atcgttttat tcggcgaagc gcgcgaacgc attgcctcat tgatcggcca
    28741 aactgttccg gtcatcaaaa agacaaaatt gcaggaagcc gtggaagttg cgtatcagga
    28801 agccgtgccc ggcgacgtcg tactattatc accgggctgt gccagttttg atgaatttgc
    28861 caattataaa gaacgaggta atttctttag aaaagtggtg gaaaatctgt aaatggaaac
    28921 cgcgtacaaa aaagaagaca attatcccga ttttgttctg ctgctggtta cgctgctgct
    28981 ggcggtggtg ggaaccgcca tgatttattc ctccagttcc atcattgccc tggagaagtt
    29041 caacgacggc cagtattttt taaagaaaca gctcgtgttt gtgtttctgg gattcacgat
    29101 catggtcgcg atgacaaaag ttccgtatac gcatttaaga aaaatcgcct atccgggaat
    29161 gctgctgtgc ctgctgttac tgtccctgtt gtttgtgccg ggtatcggca tgcgccgggg
    29221 gggcgccacc cgctggctga atctgggagg cttttctttt caggtgacgg aaatggtaaa
    29281 aatcatgatc gtgatttttt tgtcgcatct gctgacacgg aaggcttttc agataacaaa
    29341 ttttacaaag ggggtgcttg tcccgctggc tgccacgtcg gtggtcatga tgctcattct
    29401 gctggaacct gattttggaa caacggcgat tattgccacg atcatgctga tgatgctgtg
    29461 tattgcaggg gcgcagatca aacatcttgc gctgctgatc ggggccttca ttcccgtggg
    29521 catcgctctg atcctgtata aaggatacag gctgacgcga ctgaccgctt ttctggatcc
    29581 ctggaaagat gcggacaata cgggtttcca gatcattcaa tcgctgatct ctttcggttc
    29641 cggcgggaca ttcggcgtgg gtatcggcga cggcatgcag aaactatttt acctgcctga
    29701 accgcacacg gactttattc tttccattat tgccgaagag agcggtttca tcggtgtggc
    29761 gatggtcatc accatgtttg ccctttttgc ctaccgcggt ttccggatcg cgctgaacgc
    29821 ccccgatctg ttcggaagtt tgctggcgtc cgggctgacc atggtgattt gcatggaagc
    29881 attcatcaat attgccggtg tgatggggct tattcccctg aagggactgg ttctgccgtt
    29941 tttgagctat ggcggaacgg catttatcat gagtatgacg gccgtgggaa tattgctcaa
    30001 tatttccacg cagacatcgt gagagcggtg tgatgatatt atttaaaaac atgagtggct
    30061 ttgtgtggag cgaacaatgc ggattgtaat tgcgggagga ggaacaggcg gtcatttgtt
    30121 tcccgggatt gccatcgcgg aggaatttat aaaaagaaat gcacagacca aggtggtgtt
    30181 catcggaacg aaaagaggca ttgaaagccg attgctcagc cgattgggct atgacctcga
    30241 gacgattgac attgagggca tcaagggacg ggggatcagg gcattggtga aaagtgtttt
    30301 ccagattcca aaaagtttgt ggcaggccag aggaattctg aaacaattca gtcctgacat
    30361 ggtggtcggt gtcggcggtt acgcatcggg tcctgcggtg ctggcggcct atctgatggg
    30421 gatcccgacg tccattgctg aacaaaacgc catccccggt gtcaccaaca aaattctggg
    30481 aaacattgca ggcaaaatat ttgtcactta cccccggaca cagcaatgct tcccgcgtcg
    30541 aaaagttgtg atcagcggca atccggtgag ggcgtcgttt gccgcacaga gtttccgaac
    30601 gaaagaagac agaacgcacc ggcaactcct gattttcggc ggatcacagg gtgccgaagc
    30661 gatcaacagg ggcgtcatcg atatgttgcc gcggttaagc ggcatgaaag accggatacg
    30721 cattgtgcat cagaccggtg cgcggcagat ggaagaagtt aaaaaagctt acgcgcaatc
    30781 cggtcttcag gcggtagcaa cggatttcat catggatatg gctgaagcct atgcgcaggc
    30841 ggatttgatc atttgccgcg ccggtgcgac gtcgctggcg gaaataacgg ttgcgggcaa
    30901 ggccgctgtt ttgattccgt atccgtgggc ggccaatgat caccaactga aaaatgctct
    30961 ggccatgtcc gacgcgggag ccgcggtggt gattccggaa aaagagctga gtggcgaaag
    31021 cctgttcaac gccattgacg gcattctgcg tgatgaaaac cggcggctat tgatggaaga
    31081 aaattccctg aggatgggaa aagcggaggc ggcggcaacg atcgttgatg aatgcatgaa
    31141 actgacggac gccaaacatt aaagacagag tgcttggaaa gcggaaaaac aatgaaaaaa
    31201 gataaggaaa aaagcctgat gcagcgcaaa atcaaatgca tccattttgt cggaatcggc
    31261 ggaatcggca tgagcggcat tgccgaggtt ctggttaatc tcggatacaa tgtgggcggt
    31321 tcagatgttc aaccgtcgga aaccaccgcc cggctgcaga agctcggcgc taaaatcgtc
    31381 atcggacatg cggcggagaa cgtcgggaat gccgatgttg tggtgacctc aacggcggtg
    31441 aaggagcata accccgaagt gctggaagcg caccggaaaa atattccggt catcccgcgt
    31501 gcggaaatgc tggcggaact gctcaaaatg aaattttcgg tggctgtttc cgggagtcac
    31561 ggcaaaacga cgaccacgtc catgatttcc accatcctgg cggaggggaa tctggatccg
    31621 accatggtca tcgggggtaa actggccagc atcggcagca atgcccggtt gggtgacggc
    31681 gaaatcattg tggcggaagc cgatgaaagc gacggttcgt ttctgaaatt gtcgccgacc
    31741 atcgccgtca tcaccaatat cgaccgggag catctggatt attatccggg catcgccgaa
    31801 atcaaggagg cgtttctgac gtttgccaat atcgttccct tttatgggtg cacggtcctg
    31861 tgtaacgaca atattcatgt gcgggagatt cttccggcga tcaaacgacg gatgatcacc
    31921 tacggtatcg ataccccggc ggattacagc gccgaggata taaaatttac aaaaagaaaa
    31981 acgtgttaca gtctggttca taagggagag aaattgggca agatcgaatt aattgtcccc
    32041 ggtctgttca atgtttataa ttctctggcg gcgatagcgg ttgcccggga gttgaacctg
    32101 gatctggcca ccatcaaaga aggattgaaa agtttttccg gggtgcagcg ccggttggag
    32161 atcaagggaa gggtcggcga agtaactgtt cttgatgatt acggtcatca cccgacggaa
    32221 atcaaggcga cgctggcggc catgcggcaa atctggaagg acagactgat cgtcgttttt
    32281 cagccgcaca gatatacacg gacacgggcg ctttttgagg agtttacgaa agcatttgac
    32341 gaggttgaca ttctgattct caatgacatt tatccggcca gtgaagagcc gattccaggt
    32401 attaccgctg ccgcgctttg cgaggcgatc cggaaggccg gtcataagca tgtggaatat
    32461 attccgggtg cggaagcgac cgtcgaatat ctcttaaaaa cggttcatca gaaggatacg
    32521 gttgccacca tcggagcggg cagtatttat aaaataggag aaacgttcat tactcaactg
    32581 gtagccaggg gaaagaatac gtaatgacca gccattgtga gatcaggggc attttacagg
    32641 agatagatgc cgggaaagta ttgtacgacg agccgatgtc gctgcataca tcgctggccg
    32701 tgggtggaaa ggcggatgcc ctggttgtcg tggagcacga aaatcaactg gcgcgggtca
    32761 tcaggaaatt caggatggag ggcataaatt atttcacggc gggaaatctg accaatgtta
    32821 ttgtcaagga cggcggatac cggggagcga tcatattgat gacgggtatg aaggaaattc
    32881 agtgcaaacc tgtggggggc catgaaggtt atatcctggc ccagacggga gcctctctgg
    32941 ccaaggttgt cagccggtcg gtcgtggagg cgttgagcgg cgttgagttc tgttccggca
    33001 ttccgggcag tgtcggcgga gccgtctgga tgaacgcggg agcgtacgga cgggaaatga
    33061 aggatgtgat tgaatcggta acggtgctcg ataccgaagg cagcaaaaaa acaatgatcc
    33121 gggaagcgat ctctttcggt tatcgcacat cgtcttttcc ctcgggaaca atcatcctgg
    33181 gggcccggtt cagactgaaa aaaggtgatc gcgcgacaat gaaagcgaga atcagcgaaa
    33241 ttttacaatg gcgccaggca aaacatcccc tggaatatcc gagtgccgga tcggttttta
    33301 aaaatcctcc cggacatccg gctggaaaga ttattgagga catgggactc aaaggtgaaa
    33361 aggcaggcgg cgcggaaata tcgtccaaac acgccaactt tatcgtcaat aaaggtgggg
    33421 caacggctgc ggatgttctg gcgctgatcc gtctggtcca gtccagagcc agagaagaaa
    33481 aaaatatcga tctggaaacc gaggttgtcg tgatcgggga agacagatgt taaagaaaac
    33541 gggcattcaa acaaaacaaa acagaatccg ccggcgtttt acgagcttgt ccggagattt
    33601 tttcacggcg atgacattgc tgagcatggc tgttttcctc agcgtcctgt ttatttatgt
    33661 ttacaatgtg atgatgagca gcccgtattt tcagatcaag gaaatatccg tgcgcggact
    33721 gaaggaactg acggagaagg atattctggc atcggcgaat attaaaccac aacagaatct
    33781 tctggccgtg aacaaaaatt cgatcatccg ccgtgtctgt tccaatcact gggtggaaac
    33841 ggtttacgtg ggcaaggaac tgccgggaaa actcgtcctg gacgtccggg agagaattcc
    33901 tctggccctg gtaaagcggg gtggtgattt ttatctgatg gatgtgaaag gtcatgtttt
    33961 taaaagactg ggtggaagcg atgaagttga tctgccggtc attaccggtg ctgcaacagg
    34021 cgaagcaacc cgttcgccgc tgctgttgag cgcactcggt ttgatccaga ggatttctaa
    34081 atctcccgcg tatgctcatc tcggaacgat ttccgagatt catatcgatt ccgtgttcgg
    34141 gcttgcgctg gtgtccgaca acggtctgta tttgaaactg ggcacggatg attttgaaaa
    34201 taagctgagg aagttaaagg ccattctgac cgatttggaa aacaggggaa tgaagacagg
    34261 tttcttgtgt gttgatttgt cggatcattc caaagtgacg gtcaaacgca aatatgttcc
    34321 ggaaaaaacg caagatggcg atcagaataa aaattatctg atctagaaag tataaataaa
    34381 aaagaggtga aaaggctgat gggaaagaga agcaatgttt tagttggtct tgatatcgga
    34441 acgacaaaaa ccacggcgat cgtcggtgac gtaaccgaaa cgggtatcga tatcatcggg
    34501 atcggaacat cgcagaccaa agaaatgaga aaaggcacgg ttgtcaatat cgacaccatg
    34561 gtggagtcca tcaagacggc ggtggaggaa gcggagcata tgtccgggtg ccgcatcaat
    34621 tcggcatttg tcgggatttc cggaagccac attaaaggcc agaactcttt aagtatcgtt
    34681 tccattaagg gaagggaagt ggatgaaaaa gacgtgcaga gagtgattga agcttccgag
    34741 gccattgcca ttcctgtcgg acggaaaatc ctgcatattc ttccgcagag ttacgtgatt
    34801 gacggtcagg aagggatcaa ggatcccatc ggcatgtccg gtgtccgtct cgaagccaag
    34861 gttcacattg taaccggtgc gattgccgcc attcaggata ttgaaaaatc ggtgaaccgc
    34921 gtcggtctgg atattgacgg cattgtcctg gagcaacttg ccgccgggga agccgtgctc
    34981 agcagcgatg aaaaggaact gggggtcgtc ctcgtggata tcggagggtc caatacatcc
    35041 gtgaccattt tttccgaatc cagcatcaaa cacacggcca ttctgccggt cggcggcaat
    35101 tatctgacct cggatatatc caccggtctg cgtacgcctc tgagcgaagc cgaaaaaatc
    35161 aaaatcaaac acggctgtgc catgacatcc atgattccca aggatgagac catccgagtg
    35221 cccagcctcg gcggaaggga agaccgcgaa gtctcccggc agatcctggg gcgtattgtc
    35281 gaaccccgga tggaagaaat tttaaacctg gcctacaacg aagtggtgaa attcggtttt
    35341 gaggatctgc tggccgcggg cgtcgttctg accgggggga catcgttgct ggagggcatc
    35401 agcgaactgg ctgaaaagat ctttgatatg ccggcgcgca agggcattcc catcggcgtc
    35461 ggaggattga acgacattgt caattcgccg gctcatgcca tcggcgtggg actgatcatt
    35521 tacggccgta atcaaaatat tttaagagga acatccaccg ggatgcttgg aagtataaca
    35581 agaactgtta aaaaaatgtt ttcagaattt ttctgaaagg aaggagagcg catgtttgaa
    35641 ttaacggaag tctgtagtga aaatctggca aaaattaaag tgattggtgt cggcgggggc
    35701 ggcggcaatg ccgtcaacac gatgatcgca tcctgcctgc agggtgttga ttttatcaac
    35761 gccaacacgg atgcgcaggc cctcggcgtg tccagcgcac cggtcaaaat tcaaatcgga
    35821 agtcaaatca ccaaagggct cggagccggt tccgatccgg agatcgggaa acaatccgct
    35881 ctggaatccc gcgatgaaat ccgtccttat ctggaaggcg cggatatgat tttcatcacg
    35941 gcgggcatgg gcggaggaac gggaacaggc gccgctccgg tgattgcaga gattgcccgc
    36001 gagtgcagca ttctgaccat cgccgttgtg accaagccgt tccagtttga agggaaaaag
    36061 cgcaatatgc aggcggagga aggcatcgaa cagctcaggg aaaaagtgga tacgctgatc
    36121 gttgttccga atcagagatt gttaagcctg ggaggccgca acctttcgct tcttgaagcg
    36181 tttaagaagg cggatgacat tctttatcag gccgtcaagg gcatctccga cctgatttta
    36241 gtgccgggcc tgattaatct ggattttgcc gatgtcagaa gcgtcatgag caatatgggc
    36301 atggcgatta tgggtacggg catcgccagc ggtgaaaaca gagccgtcga agcggcccag
    36361 agagcgattt cgtctccgtt gctggaagac aataccattc agggcgccca cggtattctg
    36421 ctcaatatta ccggcgggtc ggacatgagc ctctatgaag tcaacgaggc ctcgtccctg
    36481 attcaggaag aggcggatga agacgccaat attatcttcg gcacggtgat tgatcagaac
    36541 atggaagatg aaatccgcat taccgttatc gccaccggtt ttgatgattt cgccaggaag
    36601 aaaaatgatt cagtcggcaa cgttacccgg ttccgcggcg tcggcggacg ggaggatctt
    36661 gctaccccga cgtttatccg tcgggaaaga aaactcaaca gcggtgaaat agccgttgtg
    36721 ggtcttggcg acgaaacgga gaatatggat attcagattc ccacgttttt aaggcgtcag
    36781 gctgactgat catgatgctt tcaccagaaa cgttatccgg gatgttccgt ttcacgggca
    36841 ggccgtgatc aagccatgga ttggaaacta aaagcagtat acaaaagcat actggagaat
    36901 gaaaccggat atgtaaaaaa aatatggggg aaagacaaca ccgtttgcct tgtttatccc
    36961 aatcactaca ggataggcat ggcgaatctg gggtttcaaa ccgtttatca actattaaat
    37021 gctcaacctt cctttctttg tgagagggca ttcctgtccg tttccggaaa cgaggccaga
    37081 tgcgtttccg gaacggcggg aatcttcagc ctggaaaatc aaaaagcatt aaccgaattt
    37141 gacatcctgg cgttttccat ctcctttgaa aacgattacc cgaatattct gaaaatcatg
    37201 gatgatgccg gtatgccgct acgatccgca gatcgctcgg aaaaatatcc tctgattctg
    37261 gggggcggta ttgctccgac gttgaacccg gagccgctgg ccgatttctt tgatctcttt
    37321 gttctggggg aagcagagga cattttgccg gtattctgcc gtttttttga agcatcgcgc
    37381 cgtctggggc agcgccggga acaatttctg aaagatgcgc aaaagcaact gcatcatgtt
    37441 tacgttccgg ggctctacga cgttcactat tcgccggaaa gaaaaatcac atcggtggtt
    37501 ccgcgggaag cggggttgcc ggaaaaaata aaagtggagc ctgtaaacga tatcaatgcg
    37561 ttccgcacac aggaggtgat cagcgcgtct tgcaccgaga tggaggacat gttcctggtc
    37621 gaggtcaacc gcggttgcgc acatggctgc agattctgtg cggcgagttt catttaccgc
    37681 ccggttcgtt tccgcggcat tgaggaaatg atcggcgcaa ttcatgacgg cctgacgcga
    37741 aaaaataaaa tcgggctcgt gggcaccgcc gtttccgatc atccggacat ccccgccgtc
    37801 tgccggtaca tcattgccca taacggccgg gcggggctcg gttccctgag aatcgaccgg
    37861 atcaatgagg agatggtcgg tctcataaaa gcaaacggca tcgagaccgt ggcactggcg
    37921 ccggaagccg gcagtcagaa gatgcgcgat atcctgaaaa agaaaatcag tgaagagcaa
    37981 atcatgtatg ccacagcctt actcgcagag catggcattg ccaatataag gctgtacttc
    38041 atgatcggcc ttccacggga agaggagtcg gacatcgatg ccatcatcgg tctgaccaaa
    38101 aaaattcagc accatgtacg tctccggttc gacgggaaaa aacagttccg gcgcttgacg
    38161 ctgagcatca atcagtttat tcccaaaccg gcaacgccgt ttcaatgggt tcctctgctc
    38221 gacgtccata tcgccggcaa aagaatcaaa aagatcaaag aggcgtttcg aaaagacaag
    38281 tctgtccgcg tgattcacga cgtcccgaaa tggaactatg ttcaggcact gcttgcgctg
    38341 ggcgaccggc gtgttggaga aatactgctg gccgtcaatc gtctggacgg caactggggc
    38401 cggggactca aagaagtcaa cgtcaatccg gatttttacg tttaccgtga gaaatctttc
    38461 gatgaaattc tgccctggga tattattgat ctgggaatat ccagaaaagc gctgatcaac
    38521 gaaaaccaaa aggccttgaa atgacgacgg atgatcaggc attgtcctgt tttgccgcga
    38581 ttcccgccgc tattttttcc tgttgctgcc actggccgta gatgatgccg ttagcacaaa
    38641 gccggttgag ttcctttttc agcgccggac cgattttccc ggcggtttcg ttttcgaagg
    38701 ccgtccgggg cagcggcatg aatgtatgag cgtgaatttt tgcgcccgac cggctcaggt
    38761 cttgtataac gttcagcgtg gaggcaatat cctcgtctgt ctccccgggc aggccgaata
    38821 tgaaatcaac tttcgccttc aaaccggcct gacgggtgac atccacggcg ttgtatatgg
    38881 ttgccacatc atattcgcgt ccggcgcaat ccagcattcg ctggctgccg gattgcgccc
    38941 cgatgatcag attgtcgttg ttggcgtact ttttcaccat cttgaccgta tgggcattga
    39001 cgtgttccgg cctgacttct gacggaaaac tgccgaagaa tattttaccg ctctttccga
    39061 ccaggcgcct gatgtccgac aacaggttct ccagtttttc attattgatt cttttgccgt
    39121 cttccgagcc gtatgaaaaa gcgttggggg aaataaatcg aaaatacgtc gaatcctgtc
    39181 ttttcattat tttgacgcaa tccagaatat tttctatact gcggtgccgc ggtgttgtgc
    39241 caaacaactg cgagacctga cagtaacggc aggcataagg acagccccgc gtaatctcga
    39301 tagggccgta tttggaaaaa tagtgcgaga tgggataaaa agtgttgatg tctgctgctt
    39361 cgcgcctgcc ggtaaaaacg tactctccgt ttttgtccag gtatgacaaa ccttttatgt
    39421 ccgaattatt ttcatcatga ttaattttgg acagtaattc cgtaagcgtt tcttctcctt
    39481 cgccgctgat tacgaaatcg aagcctagtt tcagcgtctc cagtggagcg cctgcgggat
    39541 gaggccctcc tgccagaaag acggtttcgc cggctaattt tttcaccctg ttaatcaggt
    39601 ctttgatctt caggaactga ggcgtgcaga aggagattcc aatgattgtt tctctgtgct
    39661 tttttgaaat ggcggatatt ttttgaagaa gttctttttc agaggataga aaatacacgt
    39721 cgatgttttt gatgtccgga tacttttcca gagccccggc cagcgcgttg tagctgttca
    39781 ggtttccttt gaggtagtaa aaaattaatg ccgtttcgcc atccataaat cggaaattcg
    39841 tttcattatt gaggggcatt tgtctgtgtg agacgtttcg tggcgaagcg cgatacccct
    39901 ctgacaaggg gcatcgtgaa aatcaggttc gactcgtttt aaaaaccgcg ccttgggcaa
    39961 tcgtccggag atcatgacgg ccaggcgtct caatgctctc taaatgcaca ttgagacgcc
    40021 cgaacagcca ttgatgacaa tccttgcgga agattgcctg taaacgttca ttacgccaaa
    40081 tcttcattgt ctgctgtttt gtaatcgcga aaagattcca cgcctgtcat ctcggagtac
    40141 tttcccgcag ctttttcata tccttctttt gctttggcca taagttcatc cgccttgcgg
    40201 gcaatatctt cgcgcgattc cttgccgctt ttgggtgcaa acaaaattcc caacgctacg
    40261 ccgatcaggc cgccgacaat caggcctgtg aaaaaatcac ttttgctgtc tgccataaaa
    40321 cacctcctca aatttaacaa agtccttgca gtctgacgga tcatcctccc aaacacgaac
    40381 ctcttgtcag accggaatta ccccgtattt caatacgcta gttttaattt agcataaaac
    40441 aggaaaatta gaaacggaga ccttccgaca gatctatccg gacatgattc tctttgcggg
    40501 tccgggttga gtacgaaatg caagaatatt tttctattgt gcatggacgc ctcggatttt
    40561 tattcttccg gccgcagacg atggtcaacc atgtgaggat tgctcaacgt aaatttttgt
    40621 cgtgaagaga aaccgcaaag catcgcaccg gatgatgaat ctttcgatta aaatcgacgc
    40681 atgggacata aacaaagagc atcgaatcac aagaaaagca cttgtccctt cacgcagcgt
    40741 ttttttacca gcgttccaaa atttccttgg tgagttccga acggatggcc tgatccagat
    40801 gcacgctgcc gaagaaacgg ctgcccgcca tcttcgcgtt gagcaccgaa aggccgttgg
    40861 agcgttcgtc gagccaggga acatcaatct gatcgtagga gaaatcgccg gtgaaaacca
    40921 tttttgttcc ctcggcgccg cgcgtcgcta tggctttgac atgggatggc ggaaggttct
    40981 gaatctcgtc gacgatgaca taacattttt gaatggagcg gccgcgaatc ttgtccagat
    41041 ccatcatttc gatttttccg ttggccagcc agtcgttgat cttttctttc atcctttcgt
    41101 caaccagttc tttgatggca ttgatattgt cgtagatcgg atgcatccag ggtttcattt
    41161 ttgcgtcgat gtctcccggc gaataaccca gccggtcctt gtttcccagt tggatggtgg
    41221 cacggctgac cagaatctgc cggtagtgcg acgcctgttc aaaagcggcc agcagcgcga
    41281 gaaaggtttt ggcggttccc gccttaccgg tcagcgtcac caggggaaca gttgcatcgc
    41341 gcagtaaatc catcgcgata aactggcccc agttgacatc gccgttattg gaacgcggct
    41401 tgatccccag caaatgaatg tcccggtcga taatacggaa atagtcgccc ttgcgtacgg
    41461 caacgaaggc cggtccccag acgccatcaa ggtcgcagta acagatgatg ccttcattat
    41521 acaggacctg ttccagttgc gcagccagtt tggcttcggg cagtttcaag ggaaaggttt
    41581 tatccgaaat ccagctccgt tgaagattaa tcgtcgcgcg agtggtatgg tgctgcttga
    41641 tgtctgtctg atgcattttg atggattgca catcgagacc gaaattcaga gccagggcct
    41701 ccaacgcggc atcctgagtc acgagaatga ctttggcttt gcccagtttc tttttggcat
    41761 tgagtgcggt ggcaataata cagtgatcat ttttatcgac cttcagatcg gcgtctttca
    41821 ccagattgaa atcatactga aaaaaacgga cggaatggtc ttttgcgcga aatagattac
    41881 ggacattgcg aataacgttg cgggcaatgt ccgccttgaa atcgtttcct gttttatggc
    41941 tgtccagttc ctcccacgtc gcataaggaa tcagcacaca attgcggcct ccctggcgca
    42001 atttttcaaa aacatccatt ccgtattcca gcgcgctggt atccaccaca aaatactttt
    42061 ccggaacctt ctctgccgcc ttgacggtct ccattgcgat tgcgtgcacc tcagctcttt
    42121 tggcattcgt catagaatcg ctcctgggat taatgaactg tgatttatga aaggtagaag
    42181 ttctgcgggc acacctcctg aaccggacca gccggtttcg ttattgagtt gataagaact
    42241 tcattttcgg catttccagt ataagaaacg ttatcttgtg tgtcaaggat attttgacca
    42301 caagatatgg ggcaaaacgg cattttttct taatacggca tcctcctgca gacggacaat
    42361 tccaaaacgc cgtctatttt aagtaaaaaa acgatcgtcc attgctttca ttttacaggg
    42421 aaataaagag atacttttgt acccttgccc ggttggcttt caatttccat tctaccgccg
    42481 tgggcaatca tgagatgttt aacgatggat aatcccagac ccgtgccgcc caaatcgcgt
    42541 gaacgtgatt tgtcaacgcg ataaaaacgt tcccccagac ggctgatgtc gtctttcgga
    42601 acgccgatac cggaatcctg gatcgataca accgtgtaag atcccgattc agaagcgtta
    42661 atactaatct cgccgccgtt cggggtaaac ttgacggcgt tgtccagaat attgacaaag
    42721 atctgcgtga gcctgtcccg gttcgccgta atcggaggga aattatcgct gacattatta
    42781 cgcacggata tatttttcag ttcggccttc gcttcaatca acgacagcat agtgtcgatg
    42841 gcctttctta gagaaacgct ttcaaaattc aaaacggttt gtttcgtttc aatattggaa
    42901 atcgtcagca gatcttccac aagccggttg aggcgttcgg cctgtttcaa aatgatgtcg
    42961 ataaactttt tcgtttcctc ctgattggta atcgcgccgg attgaatggt ttccagatag
    43021 ccgataattg ccgtcagcgg tgttttgatt tcatgcgtca cgttggcgac aaaatccatg
    43081 cggacctgtt ccagtttttt caggcgtgtc acatcgtgaa agacaatcat cgttttctct
    43141 tcatccgggc ggccgtgaac ggcggagagg ctgacattca gcacgacttt atccgcatgg
    43201 ccgaggataa tttcacgcga cacgctgatg cgtttttcct tgaactcctg aaatgccttc
    43261 tgcaaatcaa tattgcggaa agcttccatc agtgttttgc cgacgacatc gtcgtattga
    43321 atcgccagca tgttacttaa aaccgggctg ctgaattcga tgacgccttg ttcgtcaaga
    43381 atcagaacgc cttcgttcat gctggtgaaa gccgtcatca gcttgttctt ctcttcgttg
    43441 gcgatgttta tcttttcccg cagttcttca accagataat tgatgttatc ggccagggtt
    43501 ttggtttcat ccgacgtatc cagaatgatg gacccgaccg gatctccgcg ccgtaatttt
    43561 tcggtaaatt gttccatgat ttttatcggt tcgtacaaac gatatgaaaa gaacagtgca
    43621 attcccaacg caatcagcgc cacgacgaat aaagtcagga gaatgtactg atataccttg
    43681 tcgataacgt tttgtacgtg atgcagcggg cgcgccaatc ttacgtatcc cttgatcttc
    43741 tgtgcgtctt tgacggcgac agccatatag agcatatcga tcccgagaga atcgctgtac
    43801 cggacagatt tccctgtgcc tctgagccgg gcttcctgca cttccgggcg gttaaaatga
    43861 ctctccagca gagcaatgtc tttttccgaa tccgcaaaaa ccctgccccg ggcgtccatg
    43921 aatgtgattc gcgactggga gatttgggca acctgcttca gatggccgga tgccttttcc
    43981 agagacgtct ggtcaatcag ttgtgcatag gacaataatt ccgtttcaat ttgtgacgtc
    44041 agaacttttc tgacttcgtc ccgcacaaaa atatccagaa gaaagaaaga caggcagatg
    44101 atgaccagat aactgaaaaa tattttatag aacaggtttt ttttcactcg atatgcctct
    44161 atttttcatc cctggaaaga tgacggacgt tttcacccgt gatcatgtac gttaccatat
    44221 ccgcgatccc ctgggcatga tcggcaatcc gctccaggtt ttttgaaacg tgcataatta
    44281 ataaagcccg atgaatggat ttggaatcct ccatcataaa cgtcaaaagt tcccggaata
    44341 tctgctcgtt gagatcgtcc acttcctgtt cgatctgccg gacatcatcg gcgatctgta
    44401 aatcccgttt aatcagggca tccagcgctt tagccaccat tttctccaca atggacgaca
    44461 tattgggcaa atcgatatac ggtttgagtt tgggttcttc gttgagttgt atggcttttt
    44521 ctgcgatgtt gaccgccatg tcgccgattc gttccagatg gccggtgatt tttatggctg
    44581 tggtgatgaa tcgtaaatcg atggctgtcg gctggcgcag cgccagcatc ttaatacatt
    44641 tctcttcaat atccttgtcc attttatcaa tttcataatc attggcgata actttcttcg
    44701 ccaaatccgt gtctctgtcc gtcagagatg tcatggctga atggatggct ttttccgtca
    44761 gtgcgccgag ataaatcaga ttgtttttta tgtcctgcag ctccatctca taatgtgaac
    44821 tggtatgtct tctttcttcc atagatgatc ctccggttaa tgcttagcca aatcttcctg
    44881 taatataatc ttccgtctgt ttgacatccg gattggtaaa tattttttca gttttattat
    44941 attcaaccag attacccaga taaaaaaacg ccgtttcatc ggaaatcctg gctgcctgct
    45001 gcatattatg ggtcacgatg atgatagtat agtctttttt caactcttca atcaactctt
    45061 cgattttggc ggtcgaaatg gggtccagcg ccgacgtcgg ttcgtccatc aaaagcacat
    45121 ccggcttcat ggccagcgcc cgggcaatac agagccgctg ctgctggcct ccggagagat
    45181 tcatggcgga cttatttaga atatccttga cttcatccca gagaacggcc tgtttgaggc
    45241 tttgttccac cagtgcggcc atatcgtcca cggcgatgcc ggtgagtttc gggccatagg
    45301 caatgttgtt gtagatggtt ttcgggaagg gattgggctt ctgaaaaacc atgcctattt
    45361 tacggcggat ttcaaccgga tccacatcag gggcataaat atttttactt tcaaagagga
    45421 tttctccttc cacctttgtc ccgtcaatca gatcattcat ccggtttaaa agcctcagca
    45481 atgtggactt gccgcagccg gaagggccga tcagcgccgt cactttattt ttgacaaatg
    45541 tcattgaaat atcggtcagg gcgcggcttt cgccataata aaaattcaca ttgttcaatt
    45601 gaataattct ggaatccatt cttaccacct tttatttttc ctcatatatc cgcggatgat
    45661 aatcgcgatg agatcaatac ccaggacaac ggccaccaga acaagcactg ttccgtactg
    45721 aatgggtcgc gttgcctcga tgtttgttcc ggccgtcgcc aggacataga tatgatacgg
    45781 cagcgccatg acttcgtcaa atatcgaaga aggaagcttg gctgtgaaaa aagcggcggc
    45841 tgtaaacatg atgggggccg tttcccccgc ggcccggccg atccccagaa tggaaccggt
    45901 cagaattccc ggcagggcat tgggcaaaac gattcgataa attgtctgcc atttggaaac
    45961 accgagcgat aaagacgctt cacgaaacgt ttgcggcacg gctttgagcg cttcttccga
    46021 cgcgccgatg atcgtcggca gaattaaaaa acccagcgtt agagacccgg ccagaatact
    46081 tgagccgaac tttaaaaaaa tgacaaaaaa acctaatccg aatagcccga acacaaccga
    46141 gggaacgccg gccaggcaat tgatgccgac tttgatcaac cgcacaaccc ggccctgtct
    46201 ggcatactcc gtcagataga ttgccgaggc aatgcccagc ggcaagccga tcgcaatagc
    46261 gcccagggtc agataaatcg tccccaggat agccggcatg ataccgcctt tggtcatcgc
    46321 gtccgtcgga ggcttcgtga taaaatccca gctaattaca gaaatgccgt tgatcaggat
    46381 gtacgagatg attccgccca gcgcaagcgt tatcatcaga gccgacaggc ggacgcagga
    46441 aaaaaacaat ccttgtttaa aatagcgcca tttcatcgca tgggtttgaa gtatctgcat
    46501 cacgttacct tttatagcgt tccggatccg acctgtttaa acttgtgcga cacataatcg
    46561 gcaatcaaat taaaagccag cgtcagacag aacagcacaa tcccgatggc aaaaagcgcc
    46621 tgatagtgca ggctgcggaa aggcgtctcg cccatttccg cggcaatgct tgccggcatc
    46681 ggccgcaccg agtcaaaaag actttccgga atggcggccg ctccgccggc aaccattaac
    46741 acaaccatcg tttcaccgat ggcccgggcc atgcccagca taaccgccgt cgaaatgccg
    46801 gagagggcgg ccggcagaat gaccctggca atggtttcaa attttgtggc gcccagcgca
    46861 taagaagctt ccttgaattc ccgcgggacg gcgtaaagcg cgtcctctga gatactggat
    46921 atggtgggaa tcgccatcag cgccagaatc agcgatgcat tgacaatatt caaaccggtc
    46981 ggcaggtcga aggtttcctg catccagggg gcgacgatga ccatgccgaa aaatcccaga
    47041 acaacggacg gcagaccggc cagcagttcg ataaccgatt tgagcgtgat cttaatattc
    47101 tgcggcgcga tttcggaaat gtaaatggcc gccagcacac ccagaggaac agcgatgaac
    47161 gttgataaaa tggtgacgat caatgagccg acgatcagcg gaaaaatgcc gaaagacggc
    47221 ggatcatacg tgggatacca ctccatcccg aaaagaaaat cttttataga tatttttccg
    47281 aacagcggga tgccttcacg gaaaagagaa aatacgatca aacccagaac gaggaccgac
    47341 accagtgaaa aaataaaaaa gatgtatttg atgataattt ctttaatttg tctggtcata
    47401 acatttcact ttattgcctg atggaattct ttttcaaccc acgcttttat ttcatcgcgg
    47461 acacgtctgt attcatgagt aatgtcttca gcgttcccat taaatttgga cggatcggaa
    47521 aaacgtttat gaatatgtct atctcctcgc gggaagtggg gacacgattc ctgaacctga
    47581 tcgcaaaccg tgatgacgca atcaaaatgc atatccggga aaacgcttaa actcttcgac
    47641 tgattggcgc tgagatccat gccggtttcc ttcatcaccg tgacaacgag aggatcaatc
    47701 tgtgtgggat cggacccggc actgaatgcc gagtaacgat ccccgtaaag atgattcaaa
    47761 aacgcttcag ctatctgcga ccgcaccgca ttatgggtgc ataaaaacaa tacgtttttc
    47821 ttcatactgt cattttgcat caggttccct tttcaagcgt attggcgtca taaaatcatt
    47881 ttattttgtc aacggaacaa atccttcttt ctggacaatt ttctgccctt cggcggattt
    47941 aacaaaggct aaatacttgg ccacgtcgcc ggttgcgtta ccgtcggtgt acatgtagag
    48001 ctcgcgggaa aaaggatatt gtttggaaag cgccgtctgg atggttgccg taatgccgtt
    48061 gacctgcaag ggggtcacgc ttttgtccag atagccgatg ccgagatacc cgatggcata
    48121 acgatttttg gcaatcgccg taacgatggc gccgttggag gccagcattt gcgctctcgg
    48181 cgtgaccttg gtttttttca tgacgaaatg atcccatgat tcaaatgtgc ccgatgaaga
    48241 atcgcgggaa atgacaacga tcttcaaatc atcgccgccg acttccctcc agttggtaat
    48301 tttaccctga taaatctgac tgagttgatc aaccgataaa tttttgacct tgtttcttgg
    48361 atggacaacg ggaacaatcg catcatgcgc gaccacatgg gcaaccggat ttacattttt
    48421 ggttttggcc agcgcaatct cttccttctt gatttctcgg gatgatgtgg cgatgtgtgc
    48481 cgtcttatca atcagggctt tgatcccctc gccggaaccg ccgcccgaaa gcgacatctg
    48541 cacgtcagga tgctttttca taaaggcttc aagcgtgctc tgggttatcg gcaagacggt
    48601 ggttgatccc ttgataacga tcgatgagcc ggccagagca actgagctca tcagggctac
    48661 ggctgcgatc gttaaggctg cttttttaag aatactgaac attgaacttt cctcctgctt
    48721 tttgaatttg ccgttactct acagaattta tgttacagtt tgatgacagc ttaaagaaag
    48781 tttagaaaaa aacgcaaaaa aagcagacgg ccgattactt caggtcggca aatttatagc
    48841 caaagccgcg aacggtaaga atatatttgg gattttcaat atcctgctcg atctgggaac
    48901 ggagtcgccg gatatgcaca tcaacagcgc gatcggttat gaacgtgtcg tcaccccaga
    48961 catgatcgat aatctgattg cgggaataga cccggccggg atggcgcgat agaaagaaga
    49021 gaagcttcag ttccgtcggg cttaacgtaa cttttttacc atttaccaga acaagacacg
    49081 attcgtaatt gattttaagt cccgcgtgac tgaatttctc ttttccggcg tatagctcat
    49141 tttctttcag gcggcgcagg acgctgcgca ccctggcggt caattccttg acgctaaacg
    49201 gcttggtgac atagtcatcg gcgccgactt cgagaccggt gactttgtcc acctcatcgc
    49261 tttttgccgt cagcatgatg atcggcagac ccgacgtttc cggattgcgc cggacggttt
    49321 tacagacatc cagaccgttc attttcggca gcatcaaatc aaggataatc aaatccggct
    49381 tttgtgctct gatgatttgg agggcggact cgccatcgag agccttgacc gtagaaaatc
    49441 cctctttctc cagattatag gaaacgagat ccacgatatc tttttcatca tcgacaatca
    49501 gaatcttata catagtcatt cattgccatt ttcgggtgca aaagaaaatt aaaaatatca
    49561 aaatatgaga gcgggacaaa gcatgtgttc tttataaacc gttcataaac gtcatctgcc
    49621 cagagaatca aaaataattt ccgccggatc caaagcgggc atgccggaaa gtccggccag
    49681 ctgcatccgg caggaaccgc aatccgacac gatattgtcc atgccggttt tgctgatcag
    49741 ggagacggcg cggttgccga tctgtatcgc cgtaagagca ttttcttttt taaaaccgta
    49801 gctgccggaa agtccgcaac aggcgtcgtc gaggatttca tattccaggc cggggatcag
    49861 cgacatgagt tttgccgccg gataaccgat cttcagagcc cggagatggc agggaatatg
    49921 ataggcgatg ctgcgtctga cctcccggaa gtcgggtttg acataccctt cattgatgag
    49981 tttgatgatg tactcatgca cgtcataact gttttcagca atttttctgc cctgcggaat
    50041 ttccagaatg cccgggtaat catggagcag gcaaagcccg caggaggtgc aggtgtaaat
    50101 cacgtcgtaa ccggcgtcga tgtaatttga cagggattcg gcattttttt tcgcgaaata
    50161 tcttgccagc gtcagattcc cgttgcccag tgcgggcagg ccgcagcacc actggggcgg
    50221 cagaacaaca tcgacgttca tcgaagccag caggcggatg atttttctgc cgatgtcggg
    50281 gcggtaaaag ttgatgaaac acccgtagaa aaaagcgact ttttttctgc ccttgttgag
    50341 tcttttcttt tcccggcgca ttgtccggaa gctgaatttt ggaaagggca gatacgtcga
    50401 gatccccatt ttctgaaaaa tcttttttcc ggtctgagtt gacgcaacaa cattggccag
    50461 gggagcggtg aaagacccca gagcgctcaa ataatagtta ttggcaaaca gaagatgcgc
    50521 cggcgtttgc ggatgttcgt tgccgtatcg ctgctgggct ttcagaatga tctgtgccgg
    50581 ctccacaccg caggggcagg ccatttcgca gcgcttgcac tggctgcaca gcagcaccca
    50641 gccgtcctgc gccttgtctc tttccgagcg gaaacgctgc gcgtccggtc cggcctgttt
    50701 cggtccggga aattcgggat tgacccgaaa gaccggacag ttctccacgc aaatcgtaca
    50761 gcgtatgcaa tgttcaaatt tcatcgagca ggtactccgc gcaggattgt gctgcaacat
    50821 gggctgtggc cagcgcgaga ccgtggccgc agccgttttt cagaatttgg gtatcggcga
    50881 gaatgctgcc gcagacaaaa atatttttcc acggagcatc aagcgggcgc atggatgcgt
    50941 cggccctgat gccggctttg ccgatgttgt gattcaggga aaaataatcg ttatcaaacc
    51001 aggtatcccg ggggccggga acataaaccg gcagattgaa aatattttcc aggatgccct
    51061 cgcgtgtggc ggtcagaccg ccgctgacaa aggagcctgt acacaaaata aatgcccggg
    51121 cattgatact gttgggtctt ccttcggagg gcgtcgttac cgcttcgaca atattccccg
    51181 tcttttccat gcctgccaca ggccatgacc agtagatggt tccaccctta ttgcgaaacg
    51241 ttttcttcag cgcattgaaa agtcttctgc cgggcatgga cggcgggatg gtcggtattt
    51301 caaaaaccgg ttttcccgtc cgttccgata tcgtgtcgac gatgtctgcc gcggattcca
    51361 tgcccagcac ggcaggaaag gcgatcccgt cctcgtgaat gccgcgttgc gtcatccagg
    51421 cgatgaatct ttcgaaaaaa tccttctctt caaaacgggc ggcgatactc atggtggtgg
    51481 acacgcctgc atcatacacc gaaaaggtcg tgctgcggcg ccgggagacg atgtatccgg
    51541 gatagaaatc cttcaatccc ttgaacgtta taatatggac gctcccgtca tggttttcct
    51601 gaggcgccgc cgtcatggtg gaggggatca ggcaggaaat cttggatgtc ccgagcgcgg
    51661 aaagaatcag acgattggcg tccgcagaac cgctgtaagg caatccggcg tctttcatat
    51721 ctgcaagaaa tttttcaaga gattgtttga cagttttttc ctcaacaaga ttgtagggat
    51781 gttccggagg caagctcctt atgccggaga gtggattttt tgacagggaa cagacatcga
    51841 cgcagccagt ggaaagacac gcagtcggct ctcctttggt caccagcgcg actttcaaac
    51901 cgcgttcggc aagaagcgtt cccgcggtca gtccggctac gccggctcca atgatgacag
    51961 cgtcgtattc agtcacactc attgcggttt ctccatggac aggatgccca gatagatgta
    52021 ttcaacaagc tgttcttcac gaagctggtc tccccagagc gcgtaccgga tgcccttgaa
    52081 acgccgctgc agaaatccgc gcagcatctc catggattgt tcgggcgata tgatgctcat
    52141 atcgttcatg atgccgagcg ccctgaaggt acagaagccg ccctgacagg ggcccatgcc
    52201 cagacgcgtc cggtgagaaa tatctccgac gtttcgggtt gccgtctgtt ggataatttt
    52261 ctccacgttt tgtctggtaa caagttcgca ctcgcagaca atgttggtca tgtccgcgag
    52321 ccgtttggaa agcgggtagc cactcaactc ttcctgaccg tcgagcggaa tttccgccgt
    52381 ttcacaagct tttttcagat gaaaaattgt catgatggtg tcgaccgttt tttccgccat
    52441 cagccggaag gttgaaagtt ttccgcctac gatgctcaat agtccgtttt gatgatcgat
    52501 aatctgaaag ccgcgggtga tttcatggct gtcggctttt tttgatttca gcagaggccg
    52561 tacgcctgaa aaggccctga tgatgcgcgt tttgttgaaa tgaggaatgg tccgtccggc
    52621 ttcgctgatg attgtatcca cttcggaagc ttcgatcagg agattatcgg gattttcaat
    52681 ggccagcgat gttgtgccgg ccaggcagac ggcttcattg ggcacgataa tgtcgccgtc
    52741 cgaaggttgt cttagccggt tgatgaccat gttggtcagg cggtgattcg tgatgacgag
    52801 ggatcccttc accagcgtca aaggcacttc ggatccggcc agcgttgcaa cgatgtctgc
    52861 ccaggcgccg gtggcgttga cgatgatttt ggcgtgaatt tctttttctt cgccggtggt
    52921 ctgatcaacc gccttgacgc ccgtgcagcg gccgtgggat ttgataattt ccgttacttt
    52981 gtgccgtgtg aaaatcatgc cgccgcgcag ttcggacgtc cggatattga gcaggcagag
    53041 ccggaacgga tcaatggaac aatcgggaac ccggatggct tcctcgatgt ccggattcag
    53101 gtttggaacg agatcgatcg ccagtgttga cgaaataact tcggccggaa ttccgacgtc
    53161 ttcgcatccc cgcagaaata attcgcgaaa acgtttagaa tctcctggaa ggcgcacaaa
    53221 cagtcctccc gtttgttcaa cgcactttct gccgatcctt ctgagaataa gattttcttc
    53281 gtaacattcc cgggcggcta ccggatcggt gacggcatac ctgcctccgc tgtgcaaaag
    53341 accgtgacac gcgccggtcg cgcccgccgc gaagtcgcct ttttcaacga ggcagtgagg
    53401 aatgccgcgc aacgcaagat ctctcgcaat tccgcaccct gtcgcgcctc cgccgatgat
    53461 aatcacatcc gtcttaatca ttattattta tttttctctt gtccttaacg ttgaacattg
    53521 aactttaaac gttgaacgtt tcttattcct cttcttccca tttcatcgcc cttttaaccg
    53581 ctttcttcca gttgttataa agcgtatggc gtttatcgtc gtccatcgcc ggattaaaac
    53641 gacgatcaac gttacgtctc cgggtgattg tggactgatc tttccagaag ccggtggcca
    53701 gccccgccag ataagcagcc cccatggccg tggtttcgat gatttccggc cgctcgacgg
    53761 ggacgcccag aatatccgac tggaactgca ttaaaaaatt attggcgcag gctccgccgt
    53821 ccacacgcag ctcgcacaga tccagcccgc attcattgcg catcagttcc agaacatccc
    53881 tggtttgata ggcaatggat tccagcgtcg cgcgaatgat atgatttctc gttgttcccc
    53941 gcgtcagtcc gagaatggcg ccgcgcgcgt acatgtccca gtagggagca cctaatccga
    54001 caaaggccgg aacgacataa acgcccagcg agtcttcaac tctcgtagca aaatattcgc
    54061 tgtctttggc gtcataaata agtttcatgc cgtcgcgcag ccactggatg gccgcgccgg
    54121 cgacgaagat actgccttcc agcgcgtatt ccactttgtt gttgatgccc caggcgatgg
    54181 ttgtcagaag gccgtgctgt gaatgaacca gtttctctcc tgtcagcatg agcatgaagc
    54241 agcccgtgcc gtatgtattt ttaaccatac ccggcgtgta gcaggcctgt ccgaagagcg
    54301 ccgcctgctg atcgcccgcg tctccggcga tggcgatttc cgcgccgaag agacgggaat
    54361 ccgtatgacc gtaaaccatg ctggacggtt tgacgtccgg cagcatggag gcgggaatat
    54421 cgagttcctt taatatcttt tgatcccatt tcaatttttt gatgttgaaa agcattgtcc
    54481 gggaagcgtt gctgtaatcg gtcacatgca cctttcctct ggttaaattc cagatcaacc
    54541 aggtatcgac ggtgccaaaa agcagctctc cgtcttgcgc cttttgtcgg gcgccttcca
    54601 catggtcgag aatccatttt actttggttc cggaaaaata agcgtcaagc accagtccgg
    54661 tattttcccg gatatatttt tccaggcccc ttgccttgag ttcatcgcag atatccgcgg
    54721 tgcggcggca ctgccagacg atggcgttgt ataccggttt cccggttcgt ttttcccaga
    54781 cgacggtcgt ctctcgttga ttggttatgc cgatggcggc gatttcttcc gcggcaatag
    54841 cggtggtggc aagaagttcg ctgaccacac cgctttgcga tccccatatt tccatcgggt
    54901 catgttccac ccatccggct ttgggataaa tttgcgtaaa ttccttttgt gctgttccga
    54961 caatatttcc cttgtggtta taaaggatgg ctcttgaact ggtagtgccc tgatcaaggg
    55021 ataaaatata ggattttttc atgtctgcgc ccccggtatg atgataaaat tggctgcatt
    55081 tgtaaaagca gtattgcata atgctttgtt tttgcaaata aaaaaagttc atgttgaatg
    55141 atgaactgcg atgtgttaaa gtaatattac attgaaataa agaataattt attcaatccg
    55201 tatgaatcgc tataagaatt gccctttgaa ataaaaaaca gtctgccaag tgattgaaaa
    55261 caataagaaa aaattaaact atcaataatg tgaaaataaa ttaaattttt tcttgacaca
    55321 accacaaaga gagagtaaat agccttttct gttcatcaga gtaagtgctt ggacataagt
    55381 tgaaaacttc aatgagttaa ccgatagttt aattcgcagt tatcggacac ttccaatcgt
    55441 aactggttga acaaggtttt ttgaaagctt gacaaaagaa tctagctgct tagatttaaa
    55501 gcataggctc ttttttgata gggctctttg gaaattgaat agtgggaaaa tgaatttgtg
    55561 ggtccaaaca aatttatttg agtaacgcga aaaatagaaa gagaaatctt tcttctagga
    55621 ttaaaactgg agagtttgat cctggctcag aacaaacgct ggcggcgtgc ctaacacatg
    55681 caagtcgaac gagaaagtcg gtttcggccg atgagtaaag tggcgcacgg gtgagtaacg
    55741 cgtggataat ctacccttta gtccggaata acttgccgaa aggtgaacta ataccggatg
    55801 acatcccggg ttctccggaa accgggatca aagaaggcct ctgcttgcaa gcttttgctg
    55861 aaggatgagt ccgcgtacca ttagctagtt ggtagggtaa tggcctacca aggttatgat
    55921 ggttagctgg tctgagagga tgatcagcca cactggaact gaaacacggt ccagactcct
    55981 acgggaggca gcagtgagga atattgcgca atgggggcaa ccctgacgca gcaacgccgc
    56041 gtgagtgagg aaggttttcg gaccgtaaag ctctgtcgag cgggaagaaa tgcatgctgg
    56101 ctaatatcca tcatgcttga cggtaccgct agaggaagca ccggctaact ccgtgccagc
    56161 agccgcggta atacgggggg tgcaagc
//
LOCUS       DAOOLB010000008        54826 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_397_length_54826_cov_37.313870, whole genome shotgun sequence.
ACCESSION   DAOOLB010000008 DAOOLB010000000
VERSION     DAOOLB010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 54826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54826
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_397_length_54826_cov_37.313870"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2030
                     /locus_tag="PLF77_02015"
     CDS             <1..>2030
                     /locus_tag="PLF77_02015"
                     /inference="COORDINATES: protein motif:HMM:NF017388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilC/PilY family type IV pilus protein"
                     /protein_id="HPC07659.1"
                     /translation="VQEENYIYEVSFDPKNNSGASKEPFWTGHLKKYQIGDDGSIITP
                     HCWDAGVQLRERSAADRNMWTLKNGYMTSFNTANITAADLAVATDARRNEVVGFYRGE
                     PAYNLENWKLGDLFHTNPVAVKTPSKFFFDPRECSSTSFNSHRSAHERTAMNGNQVVI
                     AGGNDGQLHAFRAGNGTDCTSGGEELWSFIPPNLLQKIAPIAHNSHTDRASLSSHDFF
                     IDGPLQVSDVWLPASWSSGTTKSASDWKTIVTFTLGQGSGNYLWSRSSNCYSASASDF
                     SATYDATNYPYYCGLHAMNVTSSTSTTPTYLWHLMPTSSHAPYLGQAWSKMQTGRIKV
                     GDEERWVGFVGGGYSAATCSGSCNTPATGSAGKGFYVVDLRNGNIIWSYTRANNANMI
                     YSFPASPLPLDLDNDQFIDTVYLGDLGGNMWRFRLCAKDANCSECGRTDYTSSPCTSC
                     TTSDWTGSLLFTSTNAERGANMATPSNAHKQIFTMAQATYDDNHNLWIFFGTGENNDP
                     TYKPTEANPDTSETKNRLYAVKEDPNFTATYSSSQLENITSSRYTDSSEKHGWYINLS
                     TNPLTRSDTTVISNPIGEKMISDPAIFGGMVYYATYVPDQEEDSACGRSGDAFLYKLN
                     YKTGAGGDANPNDPSDIGTRTSYMGHGIGSSILVSYAPEYSDADIYATASGGA"
     assembly_gap    2031..2130
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2198..3862
                     /gene="argS"
                     /locus_tag="PLF77_02020"
     CDS             2198..3862
                     /gene="argS"
                     /locus_tag="PLF77_02020"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPC07660.1"
                     /translation="MKNKIINIMENALNRARQKGELPDVHLPYPEVEPPANREHGDYA
                     ANAAMILASQMKQNPRKIAQTILENIVDDEQIIDKAQIAGPGFINFFLKENVWHRAMK
                     VIEEQNDRYGRLDSGQGKNVQVEFVSANPTGPLHIGHARGAVVGDVIASLLHAAGYGV
                     SREYYINDAGNQMNNLGRSVLLRYREICGHPIEFPENCYQGDYIKEIARDILRQEGEK
                     YLESEESVAIPYFTAVAGKTILDEIKVDLKDFGVVFDEYFSEKELYKDDGVNQLLNDL
                     RRKGFIYSEGETLWFKSTAFGDEKDRVVIRQNGDPTYFAADIAYHHHKYSRGFDMLID
                     VWGADHHGYMPRLWAGIQALGYDKPSLKIILVQLVNLLRAGEPVAMSTRSGEFVTLRE
                     VLDEVGRDAARYNFLMRRSDSHLDFDLELAKKQSSENPVYYVQYAHARICSIIRTAKE
                     RDIALPVYSDIESAVLKEADEMILIKMLVRYPELISGAANALEPHRITFYLNELAGVF
                     HSYYNKYKVVSDDATLTAARLFLVKMVKIVLQNALNILGVNAPEEM"
     assembly_gap    4134..4233
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4234..4605
                     /locus_tag="PLF77_02025"
     CDS             <4234..4605
                     /locus_tag="PLF77_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPC07661.1"
                     /translation="AVPIKPEAITPSKEEPAKKEMAALKESSPSPAGVTESRPDQAKF
                     IVQVASLKDKSTAYRITGKIAALGFQTKIVKIDVKGQGSMFRVFATGFKEKSQAQQAV
                     KKINARTGMHCIIKKVDVRTN"
     gene            4635..5966
                     /gene="ffh"
                     /locus_tag="PLF77_02030"
     CDS             4635..5966
                     /gene="ffh"
                     /locus_tag="PLF77_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPC07662.1"
                     /translation="MFESLSEKLDNIFKKLKGRGVLNEENINASLKEIRMALLEADVN
                     FKVAKDFIEDVRVKAVGREVLESITPGQQIVKIVHDRLVELMGGSSATIKFGARIPSV
                     IMLVGLQGCGKTTTAAKLALLLSQEKKKICLVSADVYRPAAIEQLALLGKQIHTGVFE
                     DKNLKDPVEICAQAVEFAKKNGYEVLLIDTAGRLHIDNLLMEELQKIKSRVNPAEIIY
                     VADAMTGQDAVNVGVKFNELIGIDGVIMTKMDGDARGGSALSLKSVIGKPIKFVGVGE
                     KIDNLEVFHPERMASRILGMGDILSLVEKAQANIDKKTALELEKSIRKNSFSLEDFRN
                     QLVQIKKMGSIKDIMGMIPGLGKMKALKDMEPDDKQLVKITAIIDSMTRKERHNYLII
                     DGRRRKRIAMGSGTTVQDVNLLLKNYIEIRKMMKKFNKKDGIKSLMRNFPF"
     gene            6032..6286
                     /gene="rpsP"
                     /locus_tag="PLF77_02035"
     CDS             6032..6286
                     /gene="rpsP"
                     /locus_tag="PLF77_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPC07663.1"
                     /translation="MAVKIRLARMGAKGKPFYRIVAADSEFPRDGRFLEILGNYDPKK
                     NPAEVLVKEERIRKWLQKGAKATLTVANLLKAKGIEAKIK"
     gene            6372..6602
                     /locus_tag="PLF77_02040"
     CDS             6372..6602
                     /locus_tag="PLF77_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPC07664.1"
                     /translation="MKELIKYISQALVDYPDKVEVTEIIGEQTSVIELRVAKEDLGKV
                     IGKQGRTAKAIRMILSAASAKAHKRAVLEIIE"
     gene            6610..7110
                     /gene="rimM"
                     /locus_tag="PLF77_02045"
     CDS             6610..7110
                     /gene="rimM"
                     /locus_tag="PLF77_02045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPC07665.1"
                     /translation="MDLFEVGEIVKTRGLCGCLKVLSYLKSGNKISKPDVVYIEKTPG
                     QKNRFCLNKMEQSRNALFIELEGINDVNSAGHLIGGRVYFSKDVLKKLPAGEYYVHEI
                     IGLDVYSDAGDYLGKIESVFPTGSNDVYVCRKAKEEILLPAISDVIKRIDLDQRIMTV
                     KIPDGL"
     gene            7113..7853
                     /gene="trmD"
                     /locus_tag="PLF77_02050"
     CDS             7113..7853
                     /gene="trmD"
                     /locus_tag="PLF77_02050"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPC07666.1"
                     /translation="MIHFDILSIFPDMFTSPLGCSLLKKAQEKGIIHVECHDIRKWTS
                     GRHNMTDDAPYGGGCGMVMKPEPVEKALSAIRKKHQEEPTVILMTPQGEVLNQQIVTE
                     LSEKKRIVIICGRYEGVDERIRQHLVDREISIGDYILTGGELPAMVLIDAVSRLLPEF
                     LGNPESTFTESFSQNLLEYPQYTRPAVYKGWKVPEVLLSGNHSEIELWRKTESLRKTR
                     LKRPDLLKKMKLSDQERKLLEKIKLEDI"
     gene            7933..8487
                     /locus_tag="PLF77_02055"
     CDS             7933..8487
                     /locus_tag="PLF77_02055"
                     /inference="COORDINATES: protein motif:HMM:NF021455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPC07667.1"
                     /translation="MLYIVLLHYPVYDKNGRIVTTAIANMDIHDIARVAKTYGAAGFY
                     IVNPAARQRRLAQDIVNHWQEGFGAQYNPHRQNAFSLIHIRESLQNVVDDIVRRTGRK
                     PKSVATGAGPAQGDVLTFAELKDCLKKENDVYMLLFGTGSGISEKLIHESDFRLEPIK
                     GMAGYNHLSVRSAVAVIMDRIMRD"
     gene            8518..8865
                     /gene="rplS"
                     /locus_tag="PLF77_02060"
     CDS             8518..8865
                     /gene="rplS"
                     /locus_tag="PLF77_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009378341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPC07668.1"
                     /translation="MNILELTEKEQTRGDIPAFKTGDTVKVYVRIIEGQKQRIQAFEG
                     VVIRKRHGNSRANFTVRKISYGVGVERTFPLHSPIIDQIDVVSRGKVRRSRLYYLREL
                     RGKKARIKDAAKS"
     gene            8938..9555
                     /locus_tag="PLF77_02065"
     CDS             8938..9555
                     /locus_tag="PLF77_02065"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HPC07669.1"
                     /translation="MDTFEKIARKEGYHFIAGVDEVGRGPLAGPVVAAAVVFPPDYIH
                     SEIKDSKTLPSQKREKLYNVIENDALAIGMKVVDVETIDHVNILQASLQAMRDSVLEL
                     DVSPDYLLVDGLYPVPIITPQKPVVRGDSLSVSIAAASIIAKVTRDRIMEMYHRQFPQ
                     YNFRKNKGYGTREHLNAIRTFGICKIHRKSFHVKNLHQASFEFNG"
     gene            9565..9936
                     /locus_tag="PLF77_02070"
     CDS             9565..9936
                     /locus_tag="PLF77_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPC07670.1"
                     /translation="MTKIITGKKGERLAADFLTGNGYQILETNFRCPLGEIDIIARDH
                     QEIVFVEVKTRKSHALGYPEQAVGIQKQKKLSQLALWYLQAKKMSDKKARFDVVAVTL
                     AVQANEINLIKNAFDFVDAYP"
     gene            complement(9943..11319)
                     /locus_tag="PLF77_02075"
     CDS             complement(9943..11319)
                     /locus_tag="PLF77_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPC07671.1"
                     /translation="MAIRKKILCIGAGYVGGPSMAMIAHKCPDYRVTVVDINPERIDA
                     WNSEQLPVFEPGLDTIVKKTRGKNLFFRKDIENQIRDNDIIFVSVNTPTKSYGAGAHM
                     AADLQYVEKTARQILKNAQSSKIVIEKSTLPVKTALAMEKILSSRKGKIRFDVLSNPE
                     FMAEGTAVKDLENPDRVLIGSRETPGGLKARDHLIKIYTRWVPLNRIITSSIWSSELS
                     KLVANAFLAQRVSSINSISALCEKTDADIDEVARAVGMDKRIGDRFLKAGIGFGGSCF
                     KKDILNLVYLCNHYGLRDVAAYWENVLRINEYQRERFVNHILHSMFGTLSEKKICILG
                     FAFKADTGDTRESPAFYITQKLVAEQADVVISDPRALKNAAADLSGIKGNVKFVDDPY
                     RAAADCHAVLILTDWDLYRRLDYRKIYRSMIKPAFLFDGRNILDHQQCFSIGFNVYPI
                     GKRALTHF"
     gene            complement(11341..12285)
                     /locus_tag="PLF77_02080"
     CDS             complement(11341..12285)
                     /locus_tag="PLF77_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC07672.1"
                     /translation="MTKRILITGGAGFIGSHLCDKLLAQGHDVICLDNFFTGDKNNIA
                     HLLGHKRFELIRHDIINPVYLEVEEIYNLACPASPVHYQYNPIKTIKTNVMGVINVLG
                     IAKRTKAKILQASTSEVYGDPDVHPQNESYWGKVNPVGIRSCYDEGKRAAECLMMDYR
                     RQNGVNTKIVRIFNTYGPRMALHDGRVVSNFVIQALQNKVITVYGDGSHTRSFCFVDD
                     MITGLVRMMNAPDDFYGPVNLGNPEEFTILELAQTVIRLTNSRSRIEFLPLPQDDPVQ
                     RRPDISLAKAGIGWSPQISLEEGLLKTIDYFRQVLKNT"
     gene            12486..12797
                     /locus_tag="PLF77_02085"
     CDS             12486..12797
                     /locus_tag="PLF77_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07673.1"
                     /translation="MDANSITDDDKIIHDLKDELPDNAENNQEVIVVDGRSYENGSIA
                     KDVYTLVDVIEDHQIGDKIYEDILKRADEIVERIVRRIVPEIAERVIREEIEKIKNEP
                     S"
     gene            12819..13409
                     /locus_tag="PLF77_02090"
     CDS             12819..13409
                     /locus_tag="PLF77_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07674.1"
                     /translation="MTCFKNIVFLVLGMLILSGCFGNTQKSIKDETAKLSIKSIAILP
                     IETATDADVKDAQFLRTRIFEEIFFKGYPKISPEDVDQRLRQAPGNADDKTSSPPQAL
                     TDKIDAETVMSCSLKKNTQYKLFYASTTADVSCVLRRTGTDDVVWKTQTHAAEKSFAL
                     GRKRLEIKAHEAVEPLINKILKKIMETLPDGPNFIH"
     gene            13467..14015
                     /locus_tag="PLF77_02095"
     CDS             13467..14015
                     /locus_tag="PLF77_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HPC07675.1"
                     /translation="MNIPNLISLLRIILVPVIVIFLIQEEYANALIAFSIAGITDALD
                     GALARLLKKQTELGAFLDPLADKILLSTSFIALSIFGLIPGWLTVIVISRDLIIVIGI
                     ITLTMMSVTYEIKPVFISKITTALQIGTVFIALFVKIFPFELMRIDFINVLSWGTAFF
                     TVVSGLVYIFKGIKFLNHSSEK"
     gene            14098..14172
                     /locus_tag="PLF77_02100"
     tRNA            14098..14172
                     /locus_tag="PLF77_02100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14130..14132,aa:Val,seq:gac)
     gene            14271..16184
                     /gene="thrS"
                     /locus_tag="PLF77_02105"
     CDS             14271..16184
                     /gene="thrS"
                     /locus_tag="PLF77_02105"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPC07676.1"
                     /translation="MTIKITFPDHQELSFNRSTPAAEAVSAWKKEALAHTVAVKINGI
                     PADLSRPLDRDATIDLIDLSSAEGLSILRHSISHVMAQAVQDVFPGAKVSIGPSIEDG
                     FYYDFEYHETFTTDDFPKIEKRMKEITKADNPFVREELSREAAVALFEEREENYKVEL
                     INDLSHDVKTVSLYKQGGYVDLCRGPHIPSTGMIKAFKLLSVAGAYWRGNENNKMLQR
                     IYGTGFATENELQGYLTLLEEARKRDHRRLGRELDLFQVNDEAGAGLIIFHPKGMLLR
                     YLIEEWERKEHLKRGYDMVMGPQILKVDLWKKSGHFDHYRENMYFTEVDEQVYGIKPM
                     NCLSHMLIYKSKIRSYRDLPLRYFELGTVHRHEKTGVLHGLLRVRQFTQDDAHILCTP
                     EQLNSEIRGIADFVSHAMGIFGFEYEVELSTRPEKSIGTDADWDLATSALENALKDNH
                     IAYDVNAGDGAFYGPKIDFKLKDALKRKWQCATIQCDFTLPERFDLSYIGPDGEKHRP
                     VMLHRVILGAIERFMGVLIEHYAGAFPVWLSPTQCVLLTVTDKHIPYAQTVHDKLIAS
                     GIRSERYFDNEKLGYKIRQAQLQKIPYMLVIGDKEMDAGAVAPRTRDGKNMGAMPVDQ
                     FITLVHQTCDQNK"
     gene            16248..16742
                     /gene="infC"
                     /locus_tag="PLF77_02110"
     CDS             16248..16742
                     /gene="infC"
                     /locus_tag="PLF77_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPC07677.1"
                     /translation="MNREIKSANVRVISEDGKQLGVISLEEALAKAEEAGLDLVEVSA
                     SADTPVCKIMDYGKYRYKQNKKLHDAKKSQTVIQVKEIKLKPKTEEHDVQVKMKHIKK
                     FLKNHDKVKVSMMFRGREIAFTDIGRKLMGKIRDELANECIIDQEPRLEGRNMVMILS
                     PKKQ"
     gene            16761..16958
                     /gene="rpmI"
                     /locus_tag="PLF77_02115"
     CDS             16761..16958
                     /gene="rpmI"
                     /locus_tag="PLF77_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPC07678.1"
                     /translation="MPKLKTHRGAAKRFSLTAKGKIKRSKAFASHILTKKTTKRKRTL
                     RKSTIVDSANAREIKKLIPYM"
     gene            17007..17357
                     /gene="rplT"
                     /locus_tag="PLF77_02120"
     CDS             17007..17357
                     /gene="rplT"
                     /locus_tag="PLF77_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPC07679.1"
                     /translation="MSRIKRSVNARKRRKKVLKLAKGFFGARSRLLRTATEAVNKAMK
                     YAYRDRRARKREFRQLWIARINAAASDNNITYSRLIDGMKKSGIELDRKILAELAVND
                     PQGFAQVVTIAKGK"
     gene            17367..18383
                     /gene="pheS"
                     /locus_tag="PLF77_02125"
     CDS             17367..18383
                     /gene="pheS"
                     /locus_tag="PLF77_02125"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPC07680.1"
                     /translation="MMKELQQLESDALSELSTATSEEQVLTVKTKYLGRKGLLTGLLR
                     NIARVPDEEKPLFGKRCNELKNSLNARFDQAIEQYTSRNKEQALFKEKIDVTLPGRSV
                     NCGRVHPVVQIRREICSVFSSFGFSIVEGPEIELDYYNFEALNIPKDHPARDMQDTFY
                     IDENIVLRTHTSPVQIRVMEKIKPPLRILSPGKVYRRDSDVSHTPMFHQIEGLLVDKN
                     VTFGDLKGILSAFLKKIFGSSTMLRFRPSFFPFTEPSAEVDICCVMCGGKGCRVCGQS
                     GWLEILGSGMVDPAVFRNVDYDPDEYSGFAFGLGLERIAMLKYGISDIRLFFENDIRF
                     LKQF"
     gene            18398..20809
                     /gene="pheT"
                     /locus_tag="PLF77_02130"
     CDS             18398..20809
                     /gene="pheT"
                     /locus_tag="PLF77_02130"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPC07681.1"
                     /translation="MLVGLTWLKDYVDVQLTAQELADKLTMAGLEVDEIKKVGPSFSG
                     VVVAKIISVGPHPSAERLSLCDVHDGKDLYRIVCGAKNIAPGNIVPLAKVGAVIPGGY
                     TIKSSVLRGEKSDGMLCSEAELEIGSDATGIMQLSPDLVLGQPLEEALNLEDTVLDVN
                     VTPNRPDCLSMIGIAREVAALTGRKIKWPAVRIHEKAPDIHSVTSVKIIDSDLCPRYT
                     ARVIKNIAIAPSPRWMRLRLESAGLRSINNIVDITNFVMLETGQPLHAFDFRFLEEGR
                     IVVRRSLENEEFISLDGKIRTLPADTVLICDGIKPVAIGGIMGGLNSEVKEDTQTVLL
                     ESAYFRPTSIRSSSRKLGMSTDAAFRFERGIDPDGVVRALDRAAQLMVDLSGGSICKN
                     YIDEYPRKISSAGDIPLRLDRIRKIIGTKIAARDVLKILRRLDMTVKPEGKGRYLVTP
                     PTCRVDLEREIDLIEEIVRLYGYDRVPVTLPDVSVNELDVIARLDMEERLRQLLTGSG
                     FTEIVNYSFGTPSAADMLSLPATDERRNTVRIKNPLGEELSTMRTNLIYGLLDTAKRN
                     FNNGAFDLKLFEIGRIFLHRREGELPEEKTMIAGLITGKIRDDFWNSEQTVDFYTLKG
                     ALENIFCDFKIQNCRYAATTDESFLHPGKACHIVLYETVIGAVGEIHPDVLEKLDIRN
                     TIYVFEMNIDILSRVCEQNSLSFNEVSKFPAVIRDAAFVIPDPMEAEQMIDIVLSQME
                     ELLENVSIFDVYKGKGIPEGTKSLGLRFSYRANDRTLTDAEASAVHDRIVQKTVNVTG
                     AKLRS"
     gene            20844..21116
                     /locus_tag="PLF77_02135"
     CDS             20844..21116
                     /locus_tag="PLF77_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HPC07682.1"
                     /translation="MTKIDIIQNVHEKLGFSKKDSSDIVEAVFDLIKDCLANGEKVKI
                     SGFGNFMVKEKRARRGRNPQTGQEISITARRVLTFKSSQVLRKALN"
     gene            21212..21556
                     /locus_tag="PLF77_02140"
     CDS             21212..21556
                     /locus_tag="PLF77_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPC07683.1"
                     /translation="METIIPDKAYFRIGEVSKILNVEPYVIRYWETEFKTVNPMRTKT
                     AQRLYRKKDVQELLTIKNLLYAQRFTIDGAKKQLQKLRNTDEPEEKNSEKQKLFLIRI
                     KQELQHIREIVS"
     gene            complement(21605..21886)
                     /locus_tag="PLF77_02145"
     CDS             complement(21605..21886)
                     /locus_tag="PLF77_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PxxKW family cysteine-rich protein"
                     /protein_id="HPC07684.1"
                     /translation="MICKTVKAGFDCVFMTKNGCSYTNGSCQVVVEQCEGCGKIFEYE
                     SARYCKVYPDPAGKWLIGRCPTATHVKIEIKEAQKINPLKASKRASKKA"
     gene            22120..22569
                     /gene="dtd"
                     /locus_tag="PLF77_02150"
     CDS             22120..22569
                     /gene="dtd"
                     /locus_tag="PLF77_02150"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HPC07685.1"
                     /translation="MRAVIQRVDRACVSINRQVFSSINEGILILLGVEKSDTEADADY
                     ILEKAVNLRIFEDDQDKMNLSLLDTQGDMMVVSQFTLLGNCVKGRRPSFDAAEEPVQA
                     CRLYDYFVRQAKTFIPGLATGKFQEMMKIDLVNDGPVTIILDSRKMF"
     gene            22572..23060
                     /locus_tag="PLF77_02155"
     CDS             22572..23060
                     /locus_tag="PLF77_02155"
                     /inference="COORDINATES: protein motif:HMM:NF018624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HPC07686.1"
                     /translation="MDNFEIRIDKDGLWYYKGAHMFRKDILSIFYEHLRIDDSGRYLI
                     KLGPECCYLAVEDTVFVVLAVFKTKNADNRRDQIEILLNDDTCEILDMNSLRSGKENV
                     LYCRVKIGKFTARFSRKAYYQLAEFIESSEDGSRFFIPLNGQKYFIRQVQDSDACPTR
                     PS"
     gene            23204..24664
                     /gene="guaB"
                     /locus_tag="PLF77_02160"
     CDS             23204..24664
                     /gene="guaB"
                     /locus_tag="PLF77_02160"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPC07687.1"
                     /translation="MLDDTIKESLTFDDVLLVPAASNILPKDVDTTTSLTKNISLKIP
                     IVSAAMDTVTESRTAICMAREGGIGIIHRNMSIEQQAIEVDKVKKSESGMIVDPITID
                     PDRKVSDALKLMNQYSISGVPVVKKGKLVGILTNRDLRFEENLDQPVSNVMTKKNLVT
                     VSSNISLEKSKKLLHEHRIEKLLVVDDEYRLKGLITIKDIEKIRRYPNACKDSLGRLR
                     VGAAVGIIDREARVEALLAAGVDVIAIDTSHGHSAGVIKAVKATKSDFPKCELIAGNV
                     ATADGAKALMDAGVDAVKVGVGPGSICTTRIIAGVGVAQMSAIRDAYKIASKKGVPVI
                     ADGGIKYSGDIVKAIGAGAHCVMIGGLFAGTEESPGETILFQGRSYKVYRGMGSLEAM
                     KMGSRDRYYLDDIEAPLKTVPEGIEGRVPFRGSLSASILQLIGGLKAGMGYVGCRTIP
                     DMIKKARFVKITQAGLRESHVHDVIITKEAPNYWLD"
     gene            24719..28201
                     /gene="dnaE"
                     /locus_tag="PLF77_02165"
     CDS             24719..28201
                     /gene="dnaE"
                     /locus_tag="PLF77_02165"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPC07688.1"
                     /translation="MKHSQFVHLHLHTQYSLLDGMIRLDELFKKVKAYAMPAVAITDH
                     GNMFGAIDFYQQAFKNGIKPIIGTELYVAPKDRKDKTPSSIGESTRHLVVLVKNKQGY
                     KNLIKLNSLAHIEGFYYRPRVDKALLRECADGLIASSACLHGEIASWLVRGNMEEARR
                     AAREYLEIFGEENFYLEIMENGIPEQKIANQGLIELSKELSIPLIATNDCHYLERDHA
                     EAHNVLLCVQTGKTIEDKDRMTMATDQFYVRSPEEMCELFESVPEALENTVKIAERCN
                     MTFEFGKFYLPKFEVKHPEETLEAYLERKAVEGLEKRMPSILKYSTGEENSIRKKYSK
                     RLHEELEIIKKMGFAGYFLIVSDFIKHAKHNDIPVGPGRGSAAGSLVAFAIRITDIDP
                     LRYGLFFERFLNPDRISMPDIDIDFCQERRGEVIKYVTEKYGKDKVTQICTFGKMLAK
                     GVIRDVGRALNIPYGDADRIAKLVPSIPPNITLNEAFKMEPRFEEERKKNPQIDKLLK
                     LSLILEGLNRHSSVHAAGVVISDAPLMERVPLFSPKDDIVSQYTMKDIETVGLTKFDF
                     LGLKTLTVIKNTLQFIRDYKDTEVDINNLPLDDPDTYKLLMKGETDGVFQLESEGMKD
                     LLINFKPDRIEDVIALIAAYRPGPMKMIPDFIARKHGKQQITYELPQLEPILKETYGI
                     ILYQEQVMQIANAIGGYTMSQADTLRKVMGKKKVADMEKEKPRFMEGAKKQKIDDNKA
                     RIIWDQMEKFAEYGFNKSHSAAYAMITYQTAYLKAHYPVEFMAALLTSEKDNRDKIIK
                     HMTSCKDMGINILPPDINESEKGFSISGENIRFGLTAVKNVGEAAIESVIDVRETAKF
                     LSFIDFLNRVDLRKVNKRVIESLIKCGAFDSMGHKRSQLMHSYEEAMEVAQRNQKEKS
                     SNQSSFFDQLESGSSSFENGLSAYELPDMPEWDHKKLLSLEREALGFYITGHPLLPYT
                     DRLKLVTDSDSENLLIRRDKETVTVAGVVSAINERKTKRNDTMSYVTLEDLHGSINAI
                     FFGDLYKTSYNILHEEEPVIVKGTLDISGTEENQKISLIAQEVTPLAEALKNPYKQVR
                     FMVDADRMSAEDISSFISSIRKFQGKYESYMHIMNGKSETIVYLGDDLRLDINERLKK
                     EADNILGQGATIFS"
     gene            28217..28939
                     /locus_tag="PLF77_02170"
     CDS             28217..28939
                     /locus_tag="PLF77_02170"
                     /inference="COORDINATES: protein motif:HMM:NF003323.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HPC07689.1"
                     /translation="MPPYEERTYRSLIKNDHLISYNVKISESDLFIRSDTDLSDQAFQ
                     SLANHRHSLQIYIQNHSDFRTSLLPVDEDHLAPAIVRDMIDKSKICGVGPMAGVAGAI
                     SEFVGRDLLSSTKNIIIENGGDIFLQSQDTLTVSIFAGESPLSHKVHLEIKPEETPVG
                     ICTSSASVGPSLSFGSADAVCVLSPSATLADSAASAIGNRVKNNSDIKSALDFGMNIP
                     GIRGIVIICKKEMGIIGSVRLL"
     gene            complement(29202..30158)
                     /locus_tag="PLF77_02175"
     CDS             complement(29202..30158)
                     /locus_tag="PLF77_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HPC07690.1"
                     /translation="MAEDYYQVLGVEKKASADDIKKAYRKLALKWHPDKNPNNKTAEE
                     KFKKISEAYAVLSDPEKRNQYDQFGSADQFRQRYSQEDIFRNFDLDEILRSFGFGGIK
                     GGRATFRTNRKRGGYEGEYDDPFAGLFGGAMGGNRARYANMPQKGQDAEYNLSITLEE
                     SVLGAEKKISFQLENRIEDINVKIPAGISAGKKLRLPGKGLSGFNGGPSGDLYLNINV
                     LPHPIFARDGNDLHIEKTIKFTQAALGTTIDVPTLDGTTKRIKIAPGTQNNTKIRMKG
                     FGVPGLKGALKGDQFVKINVDVPRRLTDRQIKIIQQLADDGL"
     gene            complement(30212..30793)
                     /locus_tag="PLF77_02180"
     CDS             complement(30212..30793)
                     /locus_tag="PLF77_02180"
                     /inference="COORDINATES: protein motif:HMM:NF005322.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPC07691.1"
                     /translation="MSENEVKNILFAGVGGQGILRASDIICEVIMEAGFDVKKSEVHG
                     MAQRGGCVTSHVRYGKKVYSPLAEPGSIQTLVSFEKMESLRYLSFLRKDAAIVVNTEE
                     VYPPAVNMGESPYPDDAVEFLQNNYKTVISFDASKLAQKAGNIKTANVILLGALSNHM
                     NIDKSIWERVIKKSFPEKLIKVNLEAFQMGIHA"
     gene            complement(30812..32563)
                     /gene="iorA"
                     /locus_tag="PLF77_02185"
     CDS             complement(30812..32563)
                     /gene="iorA"
                     /locus_tag="PLF77_02185"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HPC07692.1"
                     /translation="MSGNEAIARGAYEAGIRFATAYPGTPSTEILENFCKYDGVYAEW
                     STNEKVAVEVAIGAALAGEKAMAVMKHVGVNVAADPVFTVSYTGTNGALVIVTADDPS
                     LHSSQNEQDNRNYARFAKIPMLEPADAAEAKHYIKMAFEISARFDTPVFFRTTTRVSH
                     SKSVVTLEEPAVYEDKTGFVYNAEKYVMVPHCARIRRAEVEKRQQALKEFAETFPENK
                     VEITNAEAGIITAGMPYNYAKEVFPGYSYLKLGMVYPLPEKMIRDFASKVKKLYVIEE
                     LDPFLEEQIKAMGIAVTGKEIFPYTSEFDPGVIRNAIEKQSIKKVSDYKVPLAARPPN
                     LCPGCPHRGLFYALKKHKVYVHGDIGCYTLSYMKPLEGLHSCICMGASIGMAHGMSKA
                     LKEKGKGKVVGVIGDSTFLHSGITPLLDMAYNQSDALIIILDNSTTAMTGMQEHPGTG
                     YTLMGIETKQVKLENLVSVLGIEHVRVVDPIDQKATTSAIKEELTKPGPSVIIARRPC
                     ALFKRSHIKPKPALKVDPDKCEGCRVCIGLNCPPISWKKGATKKGSKRKGVSYIDATL
                     CNGCGLCAQVCKFNAIS"
     gene            complement(32592..33269)
                     /locus_tag="PLF77_02190"
     CDS             complement(32592..33269)
                     /locus_tag="PLF77_02190"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC07693.1"
                     /translation="MAEKLTKQDLEGPDAFQNTVELVKDYISENKKRFYSIVTAVILA
                     SMIAFGIYMYWSNYQDSARAMYARTQSSASGSETQDMTGNNIRIFQELIEKYPHSWSA
                     RMAHYRLGNIYYSLGEYDKAIAHHKNFVSSRISDHAGIKFLALSSIGYCYEARKDYKS
                     ALEYFQKAQKGNNIGFESVGFSNVARIYELMNDRKNALENYKKALEKTADSSKAVFIK
                     YKISSLS"
     gene            complement(33262..33693)
                     /locus_tag="PLF77_02195"
     CDS             complement(33262..33693)
                     /locus_tag="PLF77_02195"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPC07694.1"
                     /translation="MSVNNVTKIRVLFADTDAMGVVYHTNYIKWFELGRNELMRQLGV
                     PYSELTKLNLNLPLIKVGCEYLKFATYDQLLAVETEFAYIRKATVGFNSKIWDEDQKH
                     LLAEGYTVHACTNHEGKLRRIPQLLLELVKKYNINTEEAYG"
     gene            complement(33683..34906)
                     /locus_tag="PLF77_02200"
     CDS             complement(33683..34906)
                     /locus_tag="PLF77_02200"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HPC07695.1"
                     /translation="MKYVILLGDGMADYPTDKLGGKTPLECAFTPFMDQIAAEGTLGL
                     VDTVPEGIHPGSDVATLSVLGYDPRENYTGRGPLEAASMGIDLGPDDVAYRCNLVTIG
                     DQDGPEAFMDDFTAGHISSEEAEIIIRDIHRHIGSDHLEFFPGVGYRHLLVWRNAPAN
                     PDTTPPHDITGKPVAEYLPRGNAAADVNAIIRRAADLLKNHPVNAKRRQAGKKEANAI
                     WLWGQGKKPRIVPLTQKYSLSGGMISAVDLLKGIGTIAGLKVCPVQGATGYIDTNYEG
                     KAKMALDVLNFMDFVFVHLEAPDEMGHEGNAEGKIRAIELFDEKIVGPILTKIGAFGH
                     YRIIVLSDHPTPLDLRTHVSDPSPFAVLSSEKKENRAPGMSFNEINAKAGNLLISPGH
                     LLMEKFIKDWKSVVG"
     gene            complement(34941..36251)
                     /locus_tag="PLF77_02205"
     CDS             complement(34941..36251)
                     /locus_tag="PLF77_02205"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HPC07696.1"
                     /translation="MKEIPIGLIGFGTIGTGVVKLLRQNEELITKKLGARLVIKKIAD
                     IDITTPREVRVHKGLLTRDAREIINDKDIPIIIELMGGYEPARTFVLEAVANGKHVVT
                     ANKALLATHGNEIFPAAQKNAVNIGFEASVGGTIPIIKTLKESLVANRIISVMGIMNG
                     TCNYILSKMTDDDKPFDMVLKEAQKLGFAEADPTFDIEGIDTAHKMAIILSLAYGRKV
                     NLKDIYLEGITKISREDISFAKELGYRIKLLAIAMLRNNAVEARIHPTMIPSKCLLAN
                     VNQNYNAFHIMGDASGPVFLFGQGAGMMPTASAVFSDVMDSARDVLSGKNGRGPLRSI
                     NESAMMPIRLIPMDHVETKYYFRFTAVDRPGVLSRISGILGKYNISIEACIQKARVKR
                     GAVPIVMTTYKAKESDVQKALKRISKLDILQGETILIRIEDDLN"
     gene            complement(36442..36855)
                     /locus_tag="PLF77_02210"
     CDS             complement(36442..36855)
                     /locus_tag="PLF77_02210"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPC07697.1"
                     /translation="MKKLIWVIPSVLFFLFAGCSAGLHSIETVQLAKTVRSWDGETLP
                     QYLQGQPEVTILRIRIPAGSKLEMHHHPVINAGVLLKGELTVVADDNKTLHLRAGDSI
                     VELVNKKHYGKNEGKEAAEIIVFYAGVENKPITVK"
     gene            complement(36901..37833)
                     /locus_tag="PLF77_02215"
     CDS             complement(36901..37833)
                     /locus_tag="PLF77_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HPC07698.1"
                     /translation="MTIKVAAIQLATAVAESEENIRRCERLSLQAVNEGAQWIALPEF
                     FNTGVSWNPNIARAIQSADGEAATFLCGFSKKYRVLIGGSFLCRLPDGQVRNRYQCYA
                     QGRLVGQHDKDIPTMWENYFYEGGDPGDIGVLGLYENVRVGAAVCWEFMRTMTARRLQ
                     NKVDVVIGGSCWWSIPTNFPLFLQKLWEPDNKRNALEVLRDNARILGVPVIHAAHCGE
                     IECAMPGLPMSYTGFYEGNAVILDAEGRILAHRKPEEGEGVVSAEILMMPKQDSREIP
                     QGYWLRKRGILPFFAWHHQRWLGRRWYARHVRGN"
     gene            complement(37838..38779)
                     /locus_tag="PLF77_02220"
     CDS             complement(37838..38779)
                     /locus_tag="PLF77_02220"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HPC07699.1"
                     /translation="MMGVRIGIIGIGGVGGYFGGKLAREYEKSDEYEIIFIARGEHLK
                     AIQKNGLQLFTREGDCVVRPRIATNNPDEAGMFDVVFFCTKSYSLENSAEAFISCIGR
                     QTVVIPLLNGVNSSQRLRRVLPHAIVLGGSVYIISHSDGPGIIKQEGGACKLTFGTDH
                     DPADDYRYILDILLQAKINAVLTDDISGVLWIKYLLMCPLGSLTSATGKTYGQIWENP
                     ELRQKARAMMMEVVAVAAAHHVVIQEHEVDKAMAMIAGFGYHSRTSMQLDFEKGRQTE
                     IDALTAYLCGAGRDYGVPTPLHDDVYAQLKSIKEPKE"
     gene            38958..41501
                     /locus_tag="PLF77_02225"
     CDS             38958..41501
                     /locus_tag="PLF77_02225"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HPC07700.1"
                     /translation="MRIQSFVVHLRFKSAILFASILCIVISGCTPLLNVSYKKSLPPM
                     EGEQVLPGLSDNVTVKRDNLGIPYIEAQSMDDLIFAMGYISACDRFTQMEGFRLVSQG
                     RLSELLGRATLEMDIYLRALNLNQVAQTLYLSASSDLRRLLNIYSDGVNAYLANVPLP
                     TTLKLAGHQPDPWRPIDSMYVFLVLTLGLSQNLQEEINLLNVAQVVAPEKLAWLFPIY
                     PDEPLPFEEMKKLKGINLSEAAQNIEQLGVIANLTNTLLLPAAAASNNWAVSGKLTKS
                     GKPIVANDTHLPLTMPSIWHMMHLKCPEIDGAGIAIAGVPGIVAGYNRHIGIGMTMVM
                     ADNQDIFLEKLQQKPDGLYFLYKNKWLKANSREESFRIKGQKKAVTRTFYETLHGVLL
                     NDILTDKPRHELVPMPLNPSLGIAYSWAGNETDRSMDVFFNIMLAKSVDEILKYTSQE
                     TSVIPLNLVMADRKNIAWQVTGRFPLRKKGRGLCPSPGWTGEYDWNGFLSPSKHPSLK
                     NPAKGYVGTANHRTVPADFPYILSSSWYYPDRGERIEQLLKNAKNYTLDDAKAMQLDT
                     YSRFVNVIKTALLEQKTLDTMSSVWQNSEKSTAAHQAMALLRDFDGNMRLDSAGAAFC
                     GAFLFSLSDNLFADELGGKNTRAYQSLLHTFLLAYSALHDHLTDRGKRSPLWDDVTTP
                     QKEERDLILAKTVLDAIGILEKRCGKNPDKWQWGKLHTYHWRTDATLFADYMDFINKT
                     GVKFLSGYVDRGPYPAPGDHTTLNVSAYHPGKDFGTWMIPAMRIIVDFGSDEPFVGIN
                     STGQLDNPSSPHYDDGITAFRLGQYQNFPFKEENIKTQYTKILTLVPKK"
     gene            complement(41890..44352)
                     /gene="recG"
                     /locus_tag="PLF77_02230"
     CDS             complement(41890..44352)
                     /gene="recG"
                     /locus_tag="PLF77_02230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPC07701.1"
                     /translation="MDALNEILQKIDQPLNFAFRDNYKNLSNIKDLGRTLITLLSKLD
                     SFAASFGREEINHEIGALREIFSDYDRQKLELKKRKIENAVKIVEKLKTIIHFLNISV
                     PERNADQKTIEKISDLKEALEKLNLPVQYLKGVGPKMASRFANKKIATVEDLLFFLPR
                     TYEDRREIRKINRLEAEKVQSAVGNVISCQYRYYGRKKILETVISDSTANLTAKWFKG
                     QMAYLLGVFKKGTRVIFTGDVRPDYVGKQMIHPDYEILDEKDEENLLNFKRIVPVYSE
                     TEGLYQKYYRKVMNAALMNYSRYVASPIPDSICEKRHLLNVHEALWSVHFPGSQEDID
                     QFISSRSEAHRRLIYDEFFFFQLGMAVKKSGRILDQGLSFKTDGTMLDKFYSSLKFDL
                     TAAQKRVIEEILNDMSKDTAMNRLLQGDVGSGKTIVSMAAMITACENSYQAAIMAPTE
                     ILAKQHFENIRLWAEPLGLKTVLLTGSMANASRGETLRRIQNGEAGIIIGTHALIQEG
                     VDFHKLGLVVIDEQHRFGVIQRATLRSKGINADVLVMTATPIPRTLAMTVYGDLDVSV
                     INEMPPGKKPVHTVVLPESKRDKVYQAIRAELAKGHQAFIVYPLVEESETLDLKDATN
                     MSRHLQQDVFPDYRVGLIHGKMKEKEKDEIMREFMENKIHILVSTTVIEVGIDVPRAS
                     LMVIEHAERFGLSQLHQLRGRVGRRDILSSCILLANYKCSADARKRLKVMETTTDGFT
                     IAEEDLAIRGPGDFLGTRQSGLPDFRIASIIRDARILSEAKEDAFALAARDPFLDRVE
                     HQILKEVLIWKWQGKLDLARTG"
     gene            44528..45577
                     /gene="purM"
                     /locus_tag="PLF77_02235"
     CDS             44528..45577
                     /gene="purM"
                     /locus_tag="PLF77_02235"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPC07702.1"
                     /translation="MTDNITYKDAGVDIDIANEFVERIKTSVKTTARKEVVSGIGGFG
                     GLFRLNSVDLKNPVLVSSTDGVGTKLRIAHLLDKHDTIGIDLVAMSVNDVVVQGAEPL
                     FFLDYLATGKIELEKSVSIIEGITEGCRQAGCTLLGGETAEMPGFYAPGEYDLAGFCV
                     GVVESDKLITGSTISVNDRIIGLASSGLHSNGFSLARKVLLEKGKLNVNDKLPGLEKT
                     VGLEMLEPTRIYVKALLNLFKKFTLKGLVHVTGGGFYENIPRIIPQACRAVIGKGSWP
                     VPPIFNIIREIGNIDEKEMFRVFNMGIGMMIIVSDKDCPAVLEYLEILGEKAYPLGLI
                     EIREDKKPQVIISES"
     gene            45587..46201
                     /gene="purN"
                     /locus_tag="PLF77_02240"
     CDS             45587..46201
                     /gene="purN"
                     /locus_tag="PLF77_02240"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPC07703.1"
                     /translation="MADALKLGILISGNGSNLQSIIDHIEQGSLRAVIQIVISNNPDA
                     YGIIRAKKHGLPFCVLKNSDFKNKEEFDSELINTLIKHDVELVTLAGFMRIISPRFLQ
                     AFPGRIMNIHPALLPSFPGLHGQRQAVTYGVKVSGCTVHFVDEGVDTGPIIIQRAVPV
                     RDDDTEDTLAARILKEEHKIYPQAIQLFADGKLEIRGRKVFIHP"
     gene            46267..47703
                     /locus_tag="PLF77_02245"
     CDS             46267..47703
                     /locus_tag="PLF77_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07704.1"
                     /translation="MYDLIVIGDDLSSHVAAAFCAINGLKTALFSEKGTAGSSVAGDL
                     TFDGTITPVSGFAENQIAYLSLKNLGIYPEVRLLNPAYQIILPSQRIDFFHDKEELLK
                     ELAREFPHLEGDLRLFYDVAEKNSNAAGQWISEHPGIQPKKMKDFFDYLKLIPRFVES
                     MFNTIRLKRLMSQNPSFRKVIQAQHALLSFKTDPLNSFFSSWQSCTPLRGTFSFAQGK
                     QDLVDSLIRKIKASDGLYLSGCDCLSIHKDKVIELNYTDDNGNTEKIESRHLIVSTKW
                     QNIGQLFDHKKTFSFGDFLRPAKTAYFPFSIFLGVNPAGLPEKLARHSAIICNPAREL
                     YADNNLIILESSAKEERMKDPAAKILLSATVFLPDHQEIWSKDNLKTISENIMNQLDH
                     FFPFLKENILFFDPDESMKLSLKLRGTIIPRYQIKNSFFTGFSAKGSKTRYANIYLTG
                     ASLLADAGFEAEILSGLNTANLLISQRK"
     gene            47707..49005
                     /locus_tag="PLF77_02250"
     CDS             47707..49005
                     /locus_tag="PLF77_02250"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPC07705.1"
                     /translation="MKDVFSISNLGKRMIVSPLQTGKFTRDGKRALFNTHLDNYRSLT
                     DKKGQPLSVELAGPREKIFFNPADTRAAIVTCGGLCPGINDVIRSITTALYFNYHVKK
                     IVGVKYGLQGLNPHCGHPLISLSPDVVKDISSFGGSILSTSRGPQDVQIIVDYLHRLN
                     INILFCIGGDGTIRAAEKITAEITRRNFKIAVIGIPKTIDNDLHLIQKSFGFDTAIEK
                     AADAIKSAHVEARGMVNGIGLIKIMGRHSGHIAAHAALAQSDANFVLIPEVPFDLEGK
                     NGFLGFLKKRMISRGHAVIIVAEGAGQDLLGTTDRSAEKDASGNARLLDIGSHLKNVI
                     ENYFKKISMEINLKYIEPSYLIRSVPANASDDIYCNMLGQYAVHAGMAGKTGILVGLM
                     KDEYVHLPLAAVASGRKIDPRGDIWLRVLETTGQPASMKN"
     gene            49187..49384
                     /gene="rpmB"
                     /locus_tag="PLF77_02255"
     CDS             49187..49384
                     /gene="rpmB"
                     /locus_tag="PLF77_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HPC07706.1"
                     /translation="MSRVCEVCGKKPSVGNAVSHANNKKKAVWYPNLQNLRCVDEKTG
                     SIKRIKVCTRCIRSGFVKKAT"
     gene            complement(49377..50165)
                     /locus_tag="PLF77_02260"
     CDS             complement(49377..50165)
                     /locus_tag="PLF77_02260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07707.1"
                     /translation="MLTHTYFLQKILGSAEIKNVEPDIFVYNIAPDLLAIHPDITSAR
                     THKLRRLQTLPVEYPKAAYVIYHLLIDDLSHYGYICTEDGDAFDIHSRGYSYRKGNPL
                     IRVIQDIHGIAGKEISCRDAVYQSHLIIEMIYDLVIVRHLRTFKTVEVMVDAIKYTVE
                     NRMEEFTETLRWLYGLEVNQIREVMRSALLFITQESMEQMMTIQGRMHLYQDKFGLPC
                     NDLLLNNELNRLFEQAMDLIDDDELFFNETADVIKESQIISFIR"
     gene            complement(50195..50416)
                     /locus_tag="PLF77_02265"
     CDS             complement(50195..50416)
                     /locus_tag="PLF77_02265"
                     /inference="COORDINATES: protein motif:HMM:NF027581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4911 domain-containing protein"
                     /protein_id="HPC07708.1"
                     /translation="MMSKYFQLDRKNIVAVQFIIEGYEGLATVTTIDAAVAVIQISIM
                     PDFLREMNCLLDELSKKYHMKELVDDRIR"
     gene            50500..52461
                     /locus_tag="PLF77_02270"
     CDS             50500..52461
                     /locus_tag="PLF77_02270"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HPC07709.1"
                     /translation="MRLVRNTSILLLLFIVSCSPIHPFIKSISESDVASFLQNAPRAE
                     EHPNAGADLLYSYSYVEFFEDGRSISRNLDRIKIFNERGRSFASKSISYREGYQKVKI
                     IFANTIKPDGKIIALDRKDIIDSSEYAGYEFYTDIKVKKFTMPAVEDGCIIEIAYEIE
                     NLKPVLSMDYSETFFCQNFFPIEEDIMEIVLPAGTKLQYENFRTDITPQITSLGNKIK
                     YVFINKKQKEIIPESRMPSLLDRDTFPQISFWTLSNWDIISQWYIKLFKEQMKSSAEL
                     ENFTRELIADQKTDEDKINAIFNFVSQNIRYIAVLLGPHTHKPHQAFEVFEKRYGDCK
                     DKTVLLLTMLKIASIQGLPALVPANGKYFDETIPSMNAFNHIIAVVPLKGKSFWLDAT
                     NETSSYDSPPFMLPTRIFLINDDGSYRFVTTPPLNDKKDYYHLAIRYKIDTEGNAAID
                     YVYNFFGKAAEAIRYSFKYAPPEQRKKFFENRGIEIRDLELGSFSDTRTPFTIKLSGM
                     IRNLAQVLDEETMVLSNIIPLDAYRDITAASIRRYPISLTQSMYAMENNTYQFPPGFK
                     IKKLPQNYIFKKPFRYRKEKYSFTGSEFRVYLETKNEEQNIPLGKMNEFKKYASELQK
                     HESAVKNIIFYKRYQNLDTRKDIDIINHK"
     gene            52481..54220
                     /gene="recJ"
                     /locus_tag="PLF77_02275"
     CDS             52481..54220
                     /gene="recJ"
                     /locus_tag="PLF77_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HPC07710.1"
                     /translation="MTTQRKLPSLKWKIEEAGNKNDGALLSKEFGIHPIIAQILLNRG
                     IRDVDKARHYLQPSLNDLHSPFLMKDMRQGVARLLKAIHNNEKIMIYGDYDADGITSV
                     VILYKFIREITPVLDYYIPDRIQEGYGLKESAINEFKKNGVSLIITVDCGISDVEQIA
                     YARSLGIDTIVLDHHEISGGLPPAAAAINPNRDDCPYPFKGLAGVGIAFNFLIALRGT
                     LNKEGFWKDNHYPNLREYLDIVTLGTIGDIAPLTDENRIFTKIGLELITEGRRPGIKA
                     LKEVSGVDNQVIDSFKASFSLIPRINAAGRIASPSDAVKLLLTDDLSEARPLAEKLDS
                     YNRHRQLMEKKILSDILEMIGKNRSLEKMNSLVFASDKWHPGVIGIVASRLVELFNRP
                     AFVISIINGVGKGSGRSISGFNIYKGIQQCEHLLLSFGGHYHAAGISIEEANIEKFSA
                     VLDELTGDIAEFSEIASQITIDTECGLEDIDPALLAQMEALAPFGSRNPEPLLLAHNV
                     KASSLVVVGNNHLKMSINSNGTSRDSIWFNMGKHLSALDGASLDIVFTPQINNWNGSA
                     DIQLKIKDVNIVS"
     gene            54249..54638
                     /locus_tag="PLF77_02280"
     CDS             54249..54638
                     /locus_tag="PLF77_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07711.1"
                     /translation="MMNVATKAALFNALVFPGWGEIYLKKYRRGLLIITGMAAGILSI
                     LLLVVQETLAVLKVTPVDKSTLSFGRLFQLSVKVMHSLNPFCILIILFFMIFLWLLSI
                     IDAYRLGKETMLEISTDADQQSASPEA"
     gene            complement(54595..>54826)
                     /locus_tag="PLF77_02285"
     CDS             complement(54595..>54826)
                     /locus_tag="PLF77_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07712.1"
                     /translation="KFNNLPLQAVVSRTGHDDLLTLRIEKISPEIGSASWEENFKKLF
                     REICTVGINTLEYIRAGEIKPQEKLIVDQRQY"
BASE COUNT        14874 a        12896 c        12761 g        14095 t
ORIGIN      
        1 gttcaggagg aaaattatat ctatgaagtg tccttcgatc cgaaaaataa ttccggtgca
       61 agcaaagaac ctttctggac cggacattta aagaaatatc agattggtga tgacggtagc
      121 attatcacgc cgcattgctg ggatgccggt gtgcaattaa gagaaagatc cgctgccgac
      181 agaaacatgt ggacactgaa aaacggctat atgacgtctt tcaataccgc caacatcacg
      241 gctgccgatc tggctgttgc caccgacgcc agacgaaatg aagtggtcgg gttttacagg
      301 ggcgaaccgg catacaatct ggaaaactgg aaactgggcg atctctttca caccaaccct
      361 gtcgctgtca agacgccttc aaaatttttc ttcgatccga gggagtgctc atcaacatcc
      421 tttaacagcc accgcagtgc tcatgaacga acggcaatga acggcaatca ggtggtgatc
      481 gccggcggca acgacggcca gttgcacgct ttccgcgcgg gaaacggtac ggattgtact
      541 tccggaggag aagaattatg gagtttcatt ccgccgaatc ttttgcagaa aattgccccc
      601 atcgcccata attcccatac cgaccgcgcg tcgctttcct cccacgattt cttcattgac
      661 ggtcccctcc aggtttcgga tgtctggctt ccggcttcct ggagcagcgg aacaactaag
      721 agcgcatcgg attggaaaac catcgtgacc tttacgttgg ggcagggctc cggaaattat
      781 ctctggagca gatcatccaa ctgttactcc gccagcgcgt ccgatttcag cgccacgtat
      841 gacgctacca actatcctta ctactgcggc cttcatgcaa tgaatgtgac cagttcgacc
      901 tccacaacgc cgacctattt atggcatttg atgccgacat cgtcgcatgc gccctatctg
      961 ggtcaggcct ggagcaagat gcagaccgga cgtattaaag tcggcgacga ggaacgatgg
     1021 gttggatttg tcggcggcgg ttacagcgcc gcaacatgtt ccggcagttg taataccccg
     1081 gctacaggca gcgcaggaaa gggtttctat gtcgttgatt tgcgaaacgg caatatcatc
     1141 tggagctata cgcgcgccaa taacgccaat atgatctatt ctttcccggc ctctccgttg
     1201 cctctggatc tggataatga ccagtttatc gataccgtat atctcggaga tctgggcgga
     1261 aacatgtggc ggttccggct ctgtgccaaa gatgcaaatt gttccgaatg cggaaggaca
     1321 gattacacct cttcgccctg tacctcctgc acaacctccg actggacggg aagccttctg
     1381 tttacgtcga ccaatgccga aagaggggca aacatggcaa cgccaagcaa tgcccataaa
     1441 cagatcttta cgatggcgca ggcaacctac gacgacaacc acaatctgtg gatattcttc
     1501 ggaaccggcg aaaacaatga tccgacttat aaaccgacgg aagccaatcc ggataccagc
     1561 gaaacaaaaa acagactcta cgctgttaag gaggacccca attttaccgc tacgtattca
     1621 tcctcacagc tggaaaacat cacgtcttcc cgttataccg attccagcga aaagcacggc
     1681 tggtacatta atctttcaac caatcctctg acgcgttcgg ataccacggt catttcaaat
     1741 cccatcggag aaaagatgat ctcggatccg gcaattttcg gcggaatggt gtattacgcc
     1801 acctatgtgc cagatcagga ggaagacagc gcctgcggta gatccggcga tgcgtttctc
     1861 tataagctca attacaaaac cggtgcggga ggagacgcga atcccaacga cccaagtgat
     1921 atcggtacca gaaccagtta tatggggcat ggcatcggct cctccattct tgtttcttat
     1981 gctccggaat actcggatgc ggatatctat gccacggcca gcggcggtgc nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcttgcgatg acaggcggca cgtgataatg
     2161 aaagctgctt gtcatttttt tacaggttgc aaaaattatg aaaaataaaa tcatcaacat
     2221 tatggaaaac gctctgaatc gtgctcggca gaaaggagag cttcccgatg tccatctgcc
     2281 ttatccggaa gtcgaaccgc ccgccaaccg cgagcacggc gattatgccg ccaatgccgc
     2341 catgattctg gcgtcgcaga tgaagcagaa tccgcggaaa atcgcgcaga cgattctgga
     2401 aaacatcgtc gatgatgaac agatcatcga taaggcccag attgccggcc ccggttttat
     2461 taatttcttt cttaaggaaa acgtttggca ccgggccatg aaggtcattg aagaacaaaa
     2521 cgatcgctac ggacgcctgg attcgggcca ggggaaaaac gttcaggtgg aatttgttag
     2581 tgcaaatccc acgggaccgc tgcatatcgg gcatgctcgc ggcgcggtgg tcggcgatgt
     2641 catcgccagt ttgttacatg cagccggtta tggcgtttcc cgcgaatatt acattaacga
     2701 tgccgggaat cagatgaata atctgggcag atcggtgctg ctgcgttacc gggagatttg
     2761 cggacacccc attgaatttc ctgaaaactg ttatcagggc gactatatca aagagattgc
     2821 ccgggatatt cttcgtcagg aaggggaaaa atatcttgag tccgaagagt cggtcgccat
     2881 tccctatttt accgccgtgg caggaaaaac cattcttgac gaaatcaagg tcgatttaaa
     2941 ggatttcggc gttgtttttg atgaatattt cagtgaaaag gaactgtata aagacgacgg
     3001 cgtaaatcaa ttgctcaatg atttgcgccg gaaaggcttt atttattccg aaggtgaaac
     3061 gctctggttt aaatcaacag ccttcggcga cgaaaaggac cgggtcgtga tccgccaaaa
     3121 cggagatccg acgtatttcg ccgcagatat tgcctatcat catcacaaat attcacgcgg
     3181 ttttgatatg ctgattgatg tctggggcgc cgatcaccac ggctacatgc cgaggctctg
     3241 ggcgggcatt caggccttgg gatacgataa gccatccctg aaaatcattc tggtgcaact
     3301 ggtaaatctt ttgcgggccg gagaaccggt ggccatgtcc acccgctccg gagaatttgt
     3361 aacgttgcgc gaagtcctcg atgaagtcgg aagagatgcc gcccgttata attttctaat
     3421 gcgacgttcc gacagccatc tggattttga tctggaactg gccaaaaaac agtccagcga
     3481 aaatccggtt tattatgtcc agtatgccca tgcccggatt tgcagcatta ttcggacggc
     3541 aaaagaacgc gatatcgctc tgcccgttta cagcgacatc gaatccgccg tgctcaaaga
     3601 agccgatgaa atgattttga tcaagatgct tgtccgttat ccggaattga tttccggagc
     3661 ggcaaacgct ctggaaccgc accgcattac attttacctc aacgaactgg ccggtgtttt
     3721 tcacagttat tataataaat ataaagtcgt ctcagacgat gccacgctga cggcggcaag
     3781 actttttttg gtgaagatgg ttaaaattgt tttacagaac gctttgaata ttctgggggt
     3841 aaacgctccg gaggaaatgt aactatttta aaattccgcg aaaatcaacc ggttgtttga
     3901 ctgtaataaa acgctgaaat tttgggaaat gtcatttttc cggtttgaaa aagattcatg
     3961 gctacgcaaa atacgaaaaa atttgaatta aaaataggaa agacgggcgt cttgattatt
     4021 cttggaggga tgaccgggct tttatgcgcg gcgtttcttc tgggcgttga tatcgggaaa
     4081 aatatcgaaa cgtacccggg agaaatcgcg tctctgccgc aaaaagcgct ggcnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcggtgc cgataaaacc tgaagcaatc
     4261 acaccctcta aagaagagcc cgccaaaaag gaaatggccg ctttaaagga atcctctcca
     4321 tcgccggcag gcgtaacgga atcccgtccc gatcaggcca aatttatcgt gcaggtggct
     4381 tcgctgaagg acaaatcaac ggcgtatcgg ataaccggga aaatcgccgc cctgggtttt
     4441 caaaccaaaa ttgtcaaaat tgacgtgaaa gggcagggaa gcatgttccg ggtttttgca
     4501 acgggattta aagaaaaaag tcaggcgcaa caggccgtca aaaaaatcaa tgccaggacg
     4561 ggcatgcatt gcataatcaa aaaagtagat gtcaggacaa actaaaataa taaggatgaa
     4621 atggttgaag ggttatgttc gaaagtcttt ctgaaaaact ggataatatt tttaaaaaac
     4681 ttaaagggcg cggcgtttta aacgaggaaa atatcaatgc ctcgctcaag gaaatccgca
     4741 tggccctcct ggaagcggat gtcaatttta aagtggccaa agatttcatc gaagatgtgc
     4801 gtgtcaaggc cgtgggccgg gaagtcctcg aaagcatcac ccccggccag cagatcgtca
     4861 agatcgttca tgaccggctg gtggaactca tgggcggcag cagcgccaca ataaaattcg
     4921 gagcgcgcat tccgtcggtc attatgctgg tcggcctgca gggctgcggt aaaacaacca
     4981 ctgccgccaa gctggccttg ctgttatccc aggaaaagaa aaaaatatgt ctggtttctg
     5041 cggacgttta tcgtccggcg gcaatcgaac agctggcgct gcttggtaaa cagatccata
     5101 ccggcgtatt tgaagataaa aacctgaaag accccgtgga gatttgcgct caggccgtgg
     5161 aattcgcgaa aaaaaacggg tatgaagtgc tgctgatcga cacggcgggc cggctgcata
     5221 tcgacaatct cctcatggaa gaactccaaa aaattaaaag cagggtcaat cccgctgaaa
     5281 ttatttatgt cgccgacgct atgaccggac aggatgccgt caatgtggga gtcaaattca
     5341 acgaattgat cggaatcgac ggcgtgatta tgaccaaaat ggacggcgat gcccgtggcg
     5401 gcagcgcttt gtccttgaaa tcggtgatcg gaaagccgat caaatttgtc ggggtgggag
     5461 aaaaaattga caatctggaa gtttttcatc ccgaaagaat ggcctcgcgt attctgggaa
     5521 tgggcgacat tctttccctg gtggaaaagg cgcaggccaa tatcgataaa aaaacggctc
     5581 tggagctcga aaaaagcatc cgcaagaaca gtttttcgtt ggaggacttt cgcaaccagc
     5641 tggtgcagat taaaaaaatg ggatccatca aggatattat gggaatgatt cccggcctgg
     5701 gaaaaatgaa agccctcaaa gatatggagc cggatgataa acaactggtc aaaattaccg
     5761 cgatcatcga ttccatgacc cgaaaggaga ggcacaatta tctgattatc gacggcaggc
     5821 ggcgcaaaag aatcgctatg ggcagcggaa ccaccgttca ggatgtgaat cttttactga
     5881 aaaattacat agaaataaga aaaatgatga aaaaatttaa taaaaaagac ggaataaaat
     5941 ctctgatgcg gaattttccc ttttaatata aattaaagta tgataaaaaa atagaaaaat
     6001 tattatttta aggaggtaat aggacaaatc aatggctgtt aaaattagac tcgcccggat
     6061 gggtgccaaa ggcaaacctt tttatcgaat tgtggcagcg gacagcgaat ttccccgcga
     6121 tggaagattt ctggaaattt taggcaatta cgatcccaag aaaaatcctg cggaagtcct
     6181 tgtcaaggaa gaacgcattc gtaaatggtt gcaaaagggt gccaaggcaa ctctgacagt
     6241 cgctaacttg ctgaaggcaa aggggataga agccaagatc aaataatttt ttaatcacaa
     6301 tgtttggggt gtgttctgta tcggaaatta tttctagaag gacttaatta aactcagcgg
     6361 aggttatgac gatgaaggaa ttgatcaaat atatatctca ggctttggta gattatccgg
     6421 ataaggtgga agtgactgaa atcatcggcg aacaaacatc tgttattgaa ctgcgcgtgg
     6481 ccaaagaaga tctcggtaaa gtcatcggca aacagggaag gaccgccaag gcgatccgga
     6541 tgattttaag cgcggcttcc gccaaggcgc ataagcgagc tgttttagaa ataatcgaat
     6601 aggcagacca tggatctctt tgaagttgga gagattgtca aaacccgcgg tctttgcggg
     6661 tgtttgaaag ttttatctta tcttaaaagc ggaaataaaa tatcaaaacc cgatgttgtg
     6721 tatattgaaa aaactccggg gcaaaaaaat cgtttttgcc tgaacaaaat ggaacaatcc
     6781 cgcaacgcgt tatttataga actcgagggg attaacgacg taaactcggc cgggcatctg
     6841 atcggcggcc gggtttattt ttctaaagat gttttaaaaa aacttccggc cggggagtat
     6901 tacgttcacg aaatcatcgg ccttgatgtt tacagcgacg cgggagatta cctcggcaaa
     6961 atcgaatcgg tttttcccac cgggagcaat gatgtgtatg tctgccggaa ggcaaaagag
     7021 gagatcctcc tgccggcaat ttccgacgtt ataaaacgga tagatcttga tcagcggatt
     7081 atgaccgtca aaattccgga tggattgtaa acgtgataca ttttgacatt ctttccattt
     7141 tcccggacat gtttacgtcg cccctcggct gcagtcttct gaaaaaagcg caggaaaaag
     7201 ggattattca tgtagaatgt catgacatac gcaaatggac cagcggcaga cataacatga
     7261 ccgatgacgc tccctacggc ggcgggtgcg gtatggtgat gaagccggaa ccggtggaaa
     7321 aggcgttgtc ggccatcaga aagaaacacc aagaagaacc gactgttatt ctgatgacgc
     7381 ctcaggggga ggttcttaat cagcagatcg taacggaact ttctgaaaaa aaaaggatcg
     7441 tcatcatctg cgggcgctat gagggcgttg atgaacgcat caggcagcac ctggtggacc
     7501 gtgaaatttc tatcggcgat tatattctga ccggagggga gttgccggca atggtgctga
     7561 ttgatgcggt ttcccgtctt ctgccggaat tccttggcaa tccggaatcg acattcaccg
     7621 aatctttttc acagaatctt ctggagtacc cccaatacac acggccggcc gtctataagg
     7681 gctggaaggt gcctgaggta ttgctgtccg gaaatcattc tgaaatcgag ctgtggcgca
     7741 aaacagaatc gttgaggaaa acccggctga agcgacccga tttgctcaaa aaaatgaagc
     7801 tctccgatca ggaaagaaaa ctgcttgaga aaattaagct ggaagatata taaaaccttg
     7861 attttcagaa atatttaatg tatgggtttc cgcttgaatg tgcgggatca atgatgtcac
     7921 gcggaaaagg aaatgcttta catcgttctt ttacattatc cggtatacga taaaaacggc
     7981 cggattgtca cgacggccat tgccaatatg gacattcacg acattgcccg ggttgccaaa
     8041 acgtatggcg ccgccggttt ttatatcgtc aacccggcag caaggcaacg ccgtctggcg
     8101 caggacattg tcaatcactg gcaggaagga ttcggtgcgc aatataatcc tcatcgtcaa
     8161 aacgctttca gcctgatcca tatcagagag agtttgcaga acgttgttga cgacatcgtc
     8221 cgtcggacag ggcgtaaacc gaagagcgta gcgacgggtg ccggtcctgc tcaaggcgat
     8281 gttttaacgt ttgccgaatt gaaagattgc ctgaaaaagg aaaatgacgt ttatatgctt
     8341 ctttttggca caggatccgg aatctccgaa aaactgattc acgagtcgga ttttaggtta
     8401 gagccgatca aggggatggc cggttataat catctgtcgg tacggtccgc cgtagccgtt
     8461 attatggaca gaatcatgag agattgattt tttaagcaaa atttgcggga ggaaaacatg
     8521 aatattttgg aattgactga gaaagaacaa accagagggg atatccccgc ttttaaaaca
     8581 ggggataccg tcaaagttta tgtcagaatt attgaaggcc agaaacagag gattcaggcg
     8641 tttgaaggtg tcgtcatccg taaaagacac ggaaacagcc gggccaattt tacggtcaga
     8701 aaaatctcct acggtgtcgg tgttgaaaga acgtttccct tgcattcgcc gatcatcgat
     8761 caaattgatg ttgtcagccg gggtaaggtg cgccgctcgc gcctctatta tttaagggaa
     8821 ttacggggca aaaaagccag aattaaagac gcggccaaat cataaaaatt gttctgcccg
     8881 agagcggcgt gccttcacag gtacgcaagc tcttggatcg gggatgtatc ttccgaaatg
     8941 gatacttttg aaaaaattgc ccgcaaagaa ggatatcatt ttattgccgg tgttgatgaa
     9001 gtgggcagag ggccgttggc cggtccggtc gttgcggctg cggtcgtgtt tcctcccgat
     9061 tatatccatt ctgagataaa agattctaaa acacttccct cccaaaaaag agaaaaatta
     9121 tacaacgtca ttgaaaacga tgccctggcc atcgggatga aggtcgttga cgttgaaacg
     9181 atcgatcatg tgaatattct gcaggcgtct ttacaggcca tgcgcgactc cgttctcgag
     9241 ctcgatgttt cgcccgatta tctgctcgtc gacggtttgt atcccgtgcc gatcatcacg
     9301 ccccagaagc ccgtggttag aggagacagc ctcagcgtct ccattgctgc tgcctcaatt
     9361 atcgccaaag tgacacggga ccggattatg gaaatgtatc atcgtcagtt tccgcaatat
     9421 aatttccgga aaaataaagg ctacggaacc cgggagcatc tcaacgccat cagaacattc
     9481 ggcatctgta aaattcaccg gaaatcattt catgttaaaa atcttcatca ggcatcattt
     9541 gagtttaacg gttaaatgcg aaacatgaca aaaatcatta ccgggaaaaa aggggagcgg
     9601 ctggcggctg attttctgac cggcaacggt tatcagatcc tggaaaccaa tttccgctgt
     9661 cctctggggg agattgacat tatcgcccgg gatcatcagg agatcgtttt tgttgaggtc
     9721 aaaacaagaa aatcgcatgc tctgggatat cccgaacagg ctgtcggcat ccaaaagcaa
     9781 aaaaaattgt ctcaattggc cttgtggtat cttcaagcca aaaaaatgtc cgacaagaaa
     9841 gcacgttttg atgtcgtggc ggtcaccctg gccgttcagg ccaatgaaat taatctgata
     9901 aaaaatgcct tcgattttgt tgacgcctac ccgtaaacta ttttaaaaat gcgtcagcgc
     9961 ccgttttccg atgggataaa cattgaaccc gatggagaag cactgctgat gatccagaat
    10021 gttgcggccg tcaaaaagaa aggccggctt gatcatggag cgatatattt tccggtaatc
    10081 cagtctgcgg tataaatccc agtccgttaa aatgagcacc gcgtgacagt ccgcggcagc
    10141 cctgtaagga tcgtcgacaa attttacatt tcccttgatt cccgacagat ctgcggcggc
    10201 atttttcaat gcccggggat cggaaataac aacgtcggcc tgttccgcca ccagtttttg
    10261 cgtaatataa aaagcggggc tttccctcgt atcgcccgta tcggccttga aagcaaaacc
    10321 caggatgcag atctttttct ccgacagcgt tccgaacatg ctgtgaagaa tatgatttac
    10381 aaaacgctcc cgctgatatt cgttgatgcg gaggacattt tcccagtagg cggcgacgtc
    10441 acgcaatccg taatgattgc acaaatacac cagattcaga atgtcttttt taaaacagga
    10501 tccgccgaaa ccgatgccgg ccttgagaaa tctatcgccg attcgtttat ccatgccgac
    10561 cgccctggcg acttcatcga tgtcggcgtc tgttttttcg cataacgcag atatggaatt
    10621 gatggaggac acccgctgcg cgaggaaggc gtttgcgacc agtttggaca attcgctgct
    10681 ccagatgctg gaggtgatga tcctgtttaa gggaacccaa cgcgtgtaaa ttttgatgag
    10741 gtgatctctc gcctttaatc cgccaggggt ctcgcgcgag ccgatcaaaa cgcggtcggg
    10801 attttccaga tctttcacgg cggtgccttc ggccatgaat tcgggattgg agagaacatc
    10861 aaaccggatc ttcccttttc gggaggataa aatcttttcc atcgccagcg cggtttttac
    10921 cggcaaggtg cttttttcga ttacgatttt tgacgattgc gcgttcttta aaatctggcg
    10981 cgcagttttt tccacatatt gcagatcggc cgccatgtgt gctccggcgc cgtagctttt
    11041 cgtcggggtg ttgacgctga cgaaaataat gtcattgtcc cggatttgat tttcaatgtc
    11101 ttttctgaaa aataaatttt tgccgcgggt tttttttaca atcgtatcga gaccgggttc
    11161 gaaaacgggc aactgctccg aattccacgc atcaatccgt tcaggattaa tatccacgac
    11221 ggtcacccgg taatcggggc atttgtgggc gatcatcgcc atggaagggc cgccgacata
    11281 accggctccg atgcaaagga ttttttttct gatagccatt tatttcttac ctctgtgcgt
    11341 ttatgtattt tttaatacct gacgaaaata gtcaatggtt ttgagcaacc cctcctcgag
    11401 actgatttga ggcgaccagc cgatgccggc ttttgccagc gagatatccg gcctgcgttg
    11461 aacaggatcg tcttgaggca ggggcaggaa ttctattctg gagcggctgt ttgtaagccg
    11521 gataacggtt tgcgcgagtt ccagaatggt aaattcttcc gggttgccga gattgaccgg
    11581 accgtaaaaa tcgtcggggg cattcatcat ccggacgagc ccggtaatca tatcgtcgac
    11641 aaaacagaat gatcgcgtgt gagaaccgtc gccgtagaca gtgatgactt tgttctgcag
    11701 ggcctgaatg acaaaattgc tcaccacgcg gccgtcatgc aacgccatcc gcggaccgta
    11761 ggtgttgaag atgcggacga ttttcgtgtt gacgccgttt tgccggcgat aatccatcat
    11821 caggcattcc gccgctcttt tgccttcatc atagcaactg cggatgccga ccggattcac
    11881 tttcccccag taggattcgt tctgcggatg gacgtccgga tcgccgtaca cctccgatgt
    11941 ggacgcctga agaattttgg cttttgtcct cttggcgatg ccgagaacat taattactcc
    12001 catgacgttg gttttgatcg ttttaatggg attgtactgg taatgaaccg gagacgcggg
    12061 acaggcaaga ttgtatattt cttccacttc cagataaaca ggattgatga tgtcatgacg
    12121 gatcagctca aaacgtttat gtcccagaag atgagcaata ttatttttat cgcctgtgaa
    12181 aaaattatcc agacagatga cgtcatgccc ctgtgccaga agtttgtcgc aaaggtgaga
    12241 acctataaaa cctgcaccac ctgtaatcag tatccgtttc gtcatttttc taattcacaa
    12301 ttattttggc gaatattaat atgaaataat tcaggaaagt caacaaataa aacagcatat
    12361 cgatggtatt ttttcatgcc gtccgattat gatatatctc ttgaactgtt ttatgctcta
    12421 tgataacaaa ccaacacgct gcaaatttac cgtgttgggc taagcgtctg gacaaggaga
    12481 tgacgatgga tgcaaattcc ataaccgatg acgataaaat catccatgat cttaaggatg
    12541 agttgccgga taatgctgaa aataatcagg aagtgattgt tgttgacgga cggagttacg
    12601 aaaacggctc catagcaaaa gacgtgtata ctctggtgga tgtcattgaa gatcatcaga
    12661 tcggggataa aatctatgaa gacattctca aaagagccga cgagatcgtg gaacgtattg
    12721 tccgcaggat cgttccggaa atagcggaaa gagtgatcag ggaagaaatt gaaaaaataa
    12781 aaaatgaacc gtcatgacga atagagttta aagaggatat gacctgtttc aaaaatatcg
    12841 tttttcttgt tctgggaatg ctgattctga gcggatgctt cggcaatacg caaaaatcaa
    12901 tcaaggatga gacggcaaaa ctcagcataa aatccattgc catccttccg atagaaaccg
    12961 ccacggatgc ggacgttaaa gacgctcagt tcctccgtac caggattttc gaagaaattt
    13021 tttttaaggg ctatcccaag atatccccgg aagacgtgga tcaacgcctg cgccaggcgc
    13081 ccggcaatgc cgatgacaag acatcttcgc cgccgcaggc tctgacggat aagatagacg
    13141 cggaaacggt catgtcctgc tccttgaaaa aaaatacgca atataaattg ttttatgcgt
    13201 cgacaaccgc tgacgtgagt tgtgtgttgc gccgcacagg aacggatgac gttgtttgga
    13261 aaacgcagac tcatgctgca gaaaagagtt ttgcgttggg ccgaaagaga ctggaaatca
    13321 aggcgcatga agcggttgaa ccgctgatca ataaaattct gaaaaaaata atggaaacat
    13381 tgcccgacgg gccgaacttt attcattaag caaagcaaaa cagcattacg aaaagaccgg
    13441 caaattattt ttgaaatgac tatgcgatga atattcccaa tctgatatct ttgctcagaa
    13501 ttatactggt cccggtgatt gtcatattcc tcattcagga agaatatgca aacgcgttga
    13561 ttgcattttc catcgccggt atcacggatg cactggacgg tgcgctggcc agactcttga
    13621 agaagcaaac cgaactgggc gcttttctgg atccgcttgc tgataaaatt cttttatcga
    13681 cgagttttat tgccttgtcc attttcggat tgattccggg atggctgact gtcattgtca
    13741 tcagcaggga tctgatcatt gtcatcggta tcatcacatt aacgatgatg tcggtaacgt
    13801 atgaaattaa accggttttt atcagcaaaa tcacaacagc tttacaaata gggaccgttt
    13861 tcattgctct ttttgttaaa atatttccat ttgaattaat gcgcattgat ttcattaacg
    13921 ttctttcgtg gggcaccgct ttttttacgg ttgtctccgg attggtatat atattcaagg
    13981 gaataaagtt tttaaatcac agctcagaaa aataggctaa taaataagac atttagcttg
    14041 acacggcaat attttgctgc tatcaacacg tcgttaaaaa aatccggttt ttaccgtagg
    14101 caagtagctc agggggagag cgccatcctg acgcggtgga tgtccagggt tcaaatccct
    14161 gcttgcctac catttttata taaattacgt cttgatttca taataacaaa gggcatcagc
    14221 aagacagtga tgccctttta aaatgaagtt aaggtgcttt gacgaatgat atgactataa
    14281 aaattacttt tccagatcat caggaattat ccttcaaccg gtcaacgccg gcggctgaag
    14341 ccgtcagtgc ctggaaaaag gaagctctgg cccataccgt ggccgtaaaa atcaacggca
    14401 tcccggctga tctcagccgt ccgcttgacc gagacgccac aattgatctc atcgacctgt
    14461 cctccgctga agggttgtct atcttgaggc acagtatatc tcacgtcatg gctcaggctg
    14521 ttcaggatgt atttccgggt gcgaaagtga gtatcggccc gtccatcgaa gacggctttt
    14581 actacgattt tgaatatcat gaaaccttca cgactgatga ttttccgaaa atcgaaaaac
    14641 ggatgaaaga aatcacaaag gcggataatc cttttgtccg ggaagagctg tcccgtgaag
    14701 cagccgttgc gttattcgag gaaagagaag aaaattacaa agttgaatta atcaacgatc
    14761 tgtctcatga cgtcaagacg gtcagccttt ataaacaggg cggctatgtt gatttatgcc
    14821 gcggccccca catcccgtca acgggaatga tcaaagcctt caagctgctc agcgttgccg
    14881 gagcctactg gcgaggcaat gaaaacaaca aaatgctgca gcgtatctac ggaactggtt
    14941 ttgctactga aaatgagctt cagggatatt tgaccctgct ggaagaagcc cgtaaaagag
    15001 atcatcggcg tctgggcagg gaactggatc tgtttcaggt caacgatgaa gccggagcag
    15061 gcctgattat tttccatccc aaggggatgc tgctgcgtta tctgattgag gaatgggaaa
    15121 gaaaggaaca tctcaaacgc ggatacgata tggtcatggg accgcagatc ctcaaggtgg
    15181 atctctggaa aaaatccggg cattttgacc attaccggga aaatatgtat tttactgaag
    15241 tggatgagca ggtttacggt atcaagccga tgaactgtct gtcccatatg ctgatctata
    15301 aatcaaaaat cagaagttat cgggatttgc cgctgcgtta ttttgaactg ggcacggtgc
    15361 atcgtcatga aaagacgggg gttctgcacg gtttgctgag agtgcgacaa ttcacccagg
    15421 atgatgctca tattttatgc acacccgaac agctgaattc ggaaatccgg ggcattgccg
    15481 attttgtcag ccatgccatg ggaattttcg gatttgaata tgaggtggaa ttaagcaccc
    15541 ggccggaaaa atcaatcggc acagacgccg attgggatct ggccacctct gcgctggaaa
    15601 acgcgctcaa ggacaatcac attgcctatg atgtgaatgc cggcgacggt gctttctacg
    15661 gcccgaagat cgatttcaag ctcaaagacg ccctgaaaag aaaatggcag tgcgccacga
    15721 tccaatgcga cttcacgctt ccggaaagat tcgatttaag ctatatcgga ccggacggag
    15781 aaaagcatcg tccggtcatg ctgcaccggg tgattctcgg cgcgattgag cgtttcatgg
    15841 gggtgctcat cgaacattac gccggcgcgt ttcccgtctg gctgtcgccg acccagtgcg
    15901 ttttgctgac ggtcaccgac aagcatatcc cttacgctca gacagtgcat gataagctga
    15961 tcgcatccgg catccgctcc gagcgatatt ttgacaatga gaaactgggg tataagatac
    16021 gacaggctca gctgcagaaa attccctata tgcttgttat cggcgacaag gaaatggacg
    16081 cgggcgccgt tgcaccccgc acacgcgacg gaaagaacat gggcgccatg cccgttgatc
    16141 agttcattac attagtacat caaacttgtg atcaaaataa ataataaacg acattcggat
    16201 aagataaatt taatttcagg aggtgaccta tagtaaagga tttaagaatc aacagagaaa
    16261 tcaaatcggc caatgttcgc gtcatcagtg aagacggtaa acagttgggc gttatttccc
    16321 tggaagaggc acttgccaag gcggaagaag ccggtctgga tttggtggag gtttccgcat
    16381 ctgccgatac cccggtctgt aaaataatgg attacgggaa gtatcgctac aagcagaaca
    16441 agaaactgca tgatgccaaa aagagccaga ccgtaattca ggttaaggaa attaaattaa
    16501 aaccgaaaac ggaagaacat gatgtgcagg tcaagatgaa acacatcaaa aagtttttga
    16561 agaatcatga taaagtaaag gtctccatga tgttcagagg cagagaaatc gcatttaccg
    16621 atatcggcag aaagctgatg ggaaaaatac gggatgaact cgccaatgag tgtatcatcg
    16681 atcaggagcc ccgtctggaa ggacgcaata tggtgatgat tctttcacca aaaaaacaat
    16741 aaatatggaa aggtgatcac atgccaaaat taaaaacaca ccgaggagct gcaaaacgct
    16801 tttcactgac cgccaaaggg aagattaaac gcagcaaagc gtttgccagt cacatcctca
    16861 cgaaaaaaac gacaaagaga aaaaggacgt taagaaaatc aacgattgtt gattcggcaa
    16921 acgcaagaga aattaaaaaa cttattccct acatgtaatg gaccggggat aatttacgag
    16981 ttatctgtaa atgaggagaa aaagaaatgt ccaggataaa aaggagcgtc aacgctcgta
    17041 aaagaagaaa aaaggttttg aaactggcca aagggttctt tggcgcccgc agccgcttgc
    17101 ttcgaacggc tacggaagcg gttaacaagg cgatgaaata cgcgtaccgt gaccgccggg
    17161 cgagaaagcg ggaattccgc cagttatgga ttgcgcgcat caatgccgcc gcaagcgata
    17221 acaacattac gtacagccgc ctgatcgacg ggatgaaaaa atccggtatt gaactggatc
    17281 gtaaaattct ggcggaactg gccgtcaatg atcctcaggg atttgcgcag gtcgtgacca
    17341 ttgccaaagg caagtaaact acggctatga tgaaagagct tcaacaactg gaaagcgatg
    17401 ctttgtcgga attaagtacc gccacgtccg aggagcaggt cttgactgtc aagacgaaat
    17461 atctgggcag aaaaggacta ttaaccggtc ttttaagaaa cattgcccgg gtgcctgatg
    17521 aagaaaaacc gctcttcggt aaacgctgca acgagttgaa aaattctctg aatgccaggt
    17581 ttgatcaggc cattgagcag tacacaagcc ggaataaaga acaagcgctc ttcaaggaaa
    17641 aaattgatgt cacgctgccg ggacgatccg tcaactgcgg acgtgtgcat cctgtggttc
    17701 agatccgcag agaaatctgt tccgttttct cgtcattcgg tttttccatc gtggaaggac
    17761 cggaaatcga actggattat tataattttg aagcactgaa tataccgaaa gatcatcccg
    17821 cgcgggacat gcaggatacc ttttatattg atgagaacat cgttttaagg acgcacacgt
    17881 cgcctgttca aatcagggtc atggaaaaga tcaagccgcc gttgagaatc ttatctcccg
    17941 gaaaagtata ccgccgcgat tccgatgtgt cgcacacgcc gatgtttcat cagatcgaag
    18001 gtctgctggt ggataaaaac gtgaccttcg gcgatctgaa gggaatcctg tccgcgtttt
    18061 tgaagaagat cttcggcagt tcaaccatgc tgcgtttccg ccccagtttt ttcccgttta
    18121 cggaaccgtc tgcggaagtg gacatctgct gtgtgatgtg cggcggcaaa ggctgcaggg
    18181 tttgcggaca aagcggctgg ctggaaattc tcggatcggg catggtcgat ccggcggttt
    18241 tcagaaatgt cgactatgat ccggacgaat attccggatt tgcttttgga ctggggctgg
    18301 aacgtattgc catgttgaaa tacggcattt ccgatatccg tttatttttc gaaaacgata
    18361 tccgtttcct caaacaattt taggagattt tttctttatg ctggtcggtc tcacgtggct
    18421 taaagattat gtcgatgtgc aattaacggc gcaggagctg gccgacaaac tgacgatggc
    18481 cgggctggaa gtggacgaaa taaaaaaagt cggtccgtcc ttcagcggcg ttgtggtggc
    18541 aaaaatcata tcggtcggcc cgcatccgtc cgcagagcgt ttgtctttgt gcgatgtgca
    18601 tgacggaaag gacctttatc ggatcgtctg tggagccaaa aatattgcac cgggaaatat
    18661 cgttccgctg gcgaaagtcg gcgcggtgat tccgggaggc tatacgatca aatcctcggt
    18721 tcttcgcggt gaaaaatcgg acggcatgct ctgttccgaa gccgaactgg aaataggaag
    18781 cgatgccacg ggcattatgc aactgtctcc ggatcttgta ctcggtcaac cgttggaaga
    18841 ggctctgaac cttgaggata cggttttgga tgtcaacgta acgcccaatc gtccggattg
    18901 tctgagcatg atcggcatcg ccagagaagt cgccgccctg accggaagaa aaatcaaatg
    18961 gcctgccgtc cggattcacg aaaaggctcc ggacatccat tccgtaacct ccgtaaaaat
    19021 tatcgattcc gatctttgcc cgcgttacac cgcccgcgtg atcaaaaata tcgccatcgc
    19081 tccgtcgccc agatggatga ggcttcgtct ggagtccgca ggactgcggt cgatcaacaa
    19141 tatcgtggat atcaccaatt ttgtaatgct ggaaacgggt cagccgctgc acgcgtttga
    19201 tttccgtttt ctggaagagg gaagaatcgt tgtccggcgg tcccttgaaa acgaagaatt
    19261 tatttcgctt gacggcaaga tacgcacgct tcccgccgat accgttttaa tctgcgacgg
    19321 catcaaaccg gtggccattg gcggcattat gggggggctg aattccgaag tcaaagaaga
    19381 cacgcaaacg gtgcttttgg aaagtgcgta ttttcgtccg acctcgattc gttcttcctc
    19441 gagaaaactg ggcatgtcga cggatgctgc atttcgtttt gaacggggaa tcgatcccga
    19501 cggtgtggtc agagcccttg accgtgccgc ccagcttatg gtcgatctgt ccggaggttc
    19561 tatctgtaaa aattatatcg atgaatatcc tcggaaaatt tcttcagccg gagatattcc
    19621 tttgcggctc gacaggatcc gcaaaattat cggcacaaaa atcgccgccc gggatgtttt
    19681 gaaaatatta cggcggctcg acatgacggt gaaaccggaa gggaagggca gatatctggt
    19741 gacgcctccg acctgcaggg tagatcttga aagagaaatc gatctgattg aggaaatcgt
    19801 cagactctac ggttacgatc gagtacctgt tacgctgccc gacgtatccg tcaacgaact
    19861 ggacgttatt gcccgtctgg atatggaaga aagacttcgt caactgttga cgggaagtgg
    19921 gtttacggaa attgtcaatt atagtttcgg tacaccctct gccgcggata tgctttccct
    19981 gcccgcaaca gatgaaagac gaaatacagt cagaatcaaa aatccgctgg gagaagagct
    20041 ctccacgatg cggacaaatc tgatttacgg gttgctggat acggccaaaa gaaatttcaa
    20101 taacggcgca ttcgatttga aactttttga aatcggccgg atatttttgc atcgacggga
    20161 aggggaattg cccgaagaga aaaccatgat tgcgggactc atcacgggaa agatcagaga
    20221 tgatttctgg aactccgaac agacggtgga cttttatact ttaaaaggag ctctggaaaa
    20281 tatcttttgt gacttcaaga ttcagaactg ccgctacgca gcaacgacgg acgagtcctt
    20341 tttgcatccg ggaaaagcct gtcacatagt tttatatgaa accgtaatcg gtgctgtcgg
    20401 agaaatacat ccggatgttc tggaaaaact ggatatccgc aatacaattt atgtatttga
    20461 aatgaatatc gatattctca gcagggtttg tgagcaaaac agcctatcct ttaacgaagt
    20521 gtcgaaattt ccggctgtta tccgcgatgc tgcgtttgtc attcccgatc cgatggaagc
    20581 agagcaaatg atagacattg tcttgagcca gatggaagaa ttgcttgaaa atgtaagtat
    20641 ttttgatgta tataagggca aaggaattcc ggaaggaaca aagagtctgg gattgcgatt
    20701 ttcttaccgc gccaacgaca gaacattgac tgacgccgaa gcaagtgccg ttcacgacag
    20761 aatcgtgcag aaaaccgtca atgtgaccgg tgcaaaacta agatcataaa atattcacta
    20821 agtaaataaa atggaggtaa aatatgacga agattgatat tattcagaat gttcatgaga
    20881 aactcggttt ttccaaaaaa gattcttcag atatcgttga agcggttttt gatcttatca
    20941 aggattgtct ggccaatggc gaaaaagtca agatatccgg cttcggcaat tttatggtca
    21001 aggagaagag agcccgccgc ggacgtaatc ctcagacagg gcaggaaatt tccattacgg
    21061 caagaagggt gctgacgttc aaatcctcac aggtgctgcg caaagccctg aattaaattt
    21121 ggacattagc cggtccggtt gatttcataa gcacggcgta tttgaaataa ttttttggtt
    21181 ttacttcagc cttaaagact ttgagaacgt tatggaaacg atcattcccg ataaagctta
    21241 ttttcgcatc ggcgaagtaa gcaaaattct caatgtggaa ccctatgtca tccggtactg
    21301 ggagactgaa ttcaaaacag tcaatcccat gagaacgaaa accgctcaaa ggctgtacag
    21361 aaaaaaggat gtgcaggaat tgctgaccat taaaaatctt ctctacgcac aacgcttcac
    21421 cattgacggc gcaaaaaaac agcttcaaaa attacgcaat acggatgagc ctgaagagaa
    21481 aaacagcgaa aaacaaaaac tcttcttgat acggatcaaa caggaattac aacacatcag
    21541 ggagattgtc agttaatata aaaaagccgg ctttcaaaag ccggcttttt tcatcttttc
    21601 gtttttaagc ttttttgctg gcgcgtttgg aggccttgag cggattaatc ttttgagctt
    21661 ctttgatttc aatttttacg tgcgtagctg tcgggcacct tccaatcagc cacttccctg
    21721 caggatcggg ataaacctta caatatctgg ccgattcata ttcaaatatt tttccgcaac
    21781 cttcacattg ttcaacaaca acctgacaac tgccgttggt ataactgcag ccgtttttag
    21841 tcataaaaac gcagtcaaag cccgctttaa ccgtcttgca aatcatggat gcatccccct
    21901 tatataattc gagtatttga cgttacgggg aaaaactatc aaggatattt tcaccaacgt
    21961 ctcggttatt taaagtttgt gtccttaaca acaaaaatta tagctgtcaa gtaaaatgaa
    22021 tttcctgaaa taaaaagaag aagggttttc tttccagaaa taacttgaat gaataacgat
    22081 ttattgataa atatataaaa attgtttacg gaagagtcaa tgcgagcagt cattcagaga
    22141 gttgaccgcg cctgtgtcag cattaatcgt caggtattct cctccatcaa cgaaggaatt
    22201 cttatattac tgggtgttga gaaatcggac accgaggcgg atgccgatta tattctggaa
    22261 aaagccgtca acctcagaat ttttgaagat gatcaggaca aaatgaacct ctctcttctc
    22321 gacacccaag gagacatgat ggttgtctcg cagttcacct tgctggggaa ttgcgtcaaa
    22381 ggaaggcggc cttcgtttga cgctgcggaa gaacctgttc aagcctgcag gctctatgac
    22441 tattttgttc gtcaggcgaa aacattcatt cccggtctgg ccacgggaaa atttcaggaa
    22501 atgatgaaaa tagacctggt caacgatggt ccggtgacca tcatactgga cagcagaaag
    22561 atgttttaaa aatggataat ttcgaaataa gaatagacaa agacggtctc tggtattata
    22621 aaggcgcgca catgtttcgc aaggatattt tgagtatctt ttacgaacat ctccggattg
    22681 atgattcggg ccggtatctg attaagctcg gtccggaatg ttgttatctg gccgtggaag
    22741 atacggtgtt tgttgtcctg gccgttttca aaacaaaaaa tgcggacaac cgccgggatc
    22801 agatcgaaat tctgttaaac gatgatacgt gcgaaatcct cgacatgaac agtctgcggt
    22861 caggcaagga aaacgtgctg tactgccgtg ttaagatcgg aaaattcacc gcccgattct
    22921 cgcgcaaggc gtattatcaa ctggctgaat tcatcgaatc atcggaagac ggcagccgtt
    22981 tctttattcc cctgaacggt caaaaatatt ttatccgtca agttcaggat tcggacgcct
    23041 gcccgacaag gccatcctga tgattaaatg gtgctgagac agacggacca tgatgacgcc
    23101 acaggcattc tgcagcgtta cggggaataa aattttctca caagagcgtt gaggctctat
    23161 attattaaca agcaaccggg atttattatc aatggaggga aaaatgctcg atgacacaat
    23221 taaggaatct ttaacgtttg acgatgttct gctggtgccg gcagcgtcca atatactgcc
    23281 gaaagatgtc gatacaacca catcgttaac caaaaacata tctttaaaaa ttcccatcgt
    23341 ttcggcggcc atggacacgg tgaccgaatc gcgcacagca atttgcatgg cccgtgaagg
    23401 cggcatcggc atcatccacc ggaatatgag tattgaacag caggccattg aagtggataa
    23461 agtcaagaaa tcggaaagcg gcatgattgt cgatcccatc accattgatc cggaccggaa
    23521 agtgagcgat gctttgaaac tcatgaatca gtattcgatt tccggcgtcc cggtcgtgaa
    23581 gaaaggaaaa ctggtaggca tcctcaccaa tcgggatctc cgttttgaag aaaatctgga
    23641 tcaaccggta tcgaatgtca tgacgaaaaa aaatcttgtc acggtatcgt ccaatatttc
    23701 tctggaaaaa tccaaaaaac ttctgcatga acaccgcatc gagaaactgc tggttgttga
    23761 tgatgaatac cgattgaagg gattgatcac catcaaggac attgaaaaga tcagacgcta
    23821 tcccaatgcc tgtaaagatt cgctcggccg attaagggtc ggtgccgcag tagggatcat
    23881 cgatcgcgaa gccagagttg aagctttgct ggccgcaggc gtcgacgtta ttgcgattga
    23941 cacgtcgcac ggacattccg caggtgtcat caaggccgtg aaagcgacca aatccgattt
    24001 tcccaaatgt gaactgatcg ccggtaatgt ggcgacggcg gacggggcga aggcattgat
    24061 ggatgccggc gtggacgctg tgaaagtcgg cgttggaccg gggtccatct gcaccacgcg
    24121 tatcattgcc ggagtgggtg ttgcacagat gtctgcaatc agagacgcct acaaaatagc
    24181 ctcgaaaaaa ggggttccgg tcattgccga tggcggcatc aaatattccg gagatatcgt
    24241 gaaagccatc ggtgccggcg ctcactgtgt catgatcggc ggattatttg ccggcacgga
    24301 ggaaagtcct ggcgaaacca ttctgtttca gggaagaagt tataaagtgt accggggcat
    24361 gggatcgctt gaagccatga aaatgggtag tcgtgaccgc tattatctcg acgatattga
    24421 agcgccctta aaaacggttc cggaaggcat agaagggcgt gtgccgttcc gcggttcctt
    24481 atcggcgagt attcttcagt tgatcggcgg cctgaaagcg ggcatgggat acgtcggttg
    24541 cagaaccatt ccggacatga ttaaaaaagc gcgttttgta aaaattacgc aggccggtct
    24601 tagagaaagc catgtccatg acgtgatcat cactaaggaa gcgcccaact actggttgga
    24661 ttaagtcagt tgctttctgc tttttttatt ttttcattat ttcccaagta cggcaattat
    24721 gaaacactct cagtttgtgc atcttcatct tcatacgcaa tacagtcttc tggacgggat
    24781 gatccgtttg gatgagcttt tcaaaaaagt taaagcatat gccatgcccg ccgttgccat
    24841 tacggatcac gggaacatgt tcggagcgat agatttttat caacaggcgt ttaaaaacgg
    24901 gattaaaccc atcatcggca ctgaacttta cgtcgccccg aaagaccgta aagacaaaac
    24961 cccgtcgtcc atcggcgaat caacgcgcca tctggttgtt ctagtcaaaa acaagcaggg
    25021 gtataaaaac ctgattaaat taaactcgct cgcacatatt gaaggatttt actaccgtcc
    25081 ccgcgtggat aaggccctgc tgcgggaatg tgcggacggc ctgattgctt cgagcgcctg
    25141 cctgcatgga gagatcgcct catggcttgt ccggggaaac atggaagagg cgagaagagc
    25201 cgcccgggaa tacctggaaa ttttcggaga agagaatttt tatctggaaa tcatggaaaa
    25261 cggaattccc gagcaaaaga tcgccaatca ggggctgatt gagctgagca aggaactttc
    25321 cattccgttg attgccacca atgactgtca ttatctggaa cgggaccatg cggaagccca
    25381 caatgtgctt ttatgcgtcc aaaccggcaa aaccattgaa gacaaagatc gcatgaccat
    25441 ggcaacggat cagttttatg tccgatcacc ggaggaaatg tgcgagcttt tcgagtctgt
    25501 tcccgaagcg ctggaaaaca cggtcaaaat agccgagcga tgcaacatga ctttcgaatt
    25561 cgggaaattc tatctgccga aatttgaagt gaagcatccg gaggaaaccc tggaggctta
    25621 tctggagcgc aaggccgttg aaggtcttga aaaacgcatg ccgagcatcc tgaaatattc
    25681 aacgggagag gaaaacagca tccgcaaaaa atatagcaag cgtctccacg aagagctgga
    25741 aatcatcaaa aaaatggggt ttgccggata ttttctcatc gtttctgatt ttattaaaca
    25801 tgccaagcac aacgatattc ccgtcggtcc gggccggggg tctgcggcag gatctctcgt
    25861 ggcttttgcc atccggatta cggatatcga tcctcttcgg tatggcctat tctttgaacg
    25921 attcctgaat ccggaccgca tcagtatgcc ggatatcgat attgatttct gtcaggaacg
    25981 ccggggcgaa gttataaaat atgtgacgga aaaatacgga aaggacaagg taacccagat
    26041 atgcacattc ggtaaaatgc tggccaaagg tgtgatccgg gatgtcggcc gtgccttgaa
    26101 cattccttac ggcgacgcag accggattgc caaactggtt ccctctattc cgcccaacat
    26161 taccctgaac gaagccttca aaatggagcc ccgttttgag gaagaacgga aaaagaatcc
    26221 gcaaatcgac aaactcttga aattatcact cattctggaa ggcttgaacc gtcattcttc
    26281 cgtacatgcc gcaggtgtgg tgatctcgga cgctccgctg atggaacgag tcccactctt
    26341 ttccccgaag gacgatatcg tttctcaata caccatgaaa gacattgaaa cggtcgggct
    26401 gacgaaattc gatttcctgg gcttgaaaac attaaccgtt atcaagaaca ctctccagtt
    26461 catccgcgac tataaagaca cggaggtgga tattaacaac ctgccgctgg atgatcccga
    26521 cacatacaaa ctgctcatga agggagaaac cgacggtgtt tttcagctgg aaagcgaggg
    26581 gatgaaggat ctgctgatca atttcaaacc cgatcgcatc gaagacgtga tcgcgctgat
    26641 tgccgcgtat cgtccgggtc ccatgaagat gatcccggat tttattgccc gcaagcacgg
    26701 caaacagcaa atcacatatg agcttccgca gcttgagccg atcctgaaag aaacgtacgg
    26761 cattattctt tatcaggagc aggtcatgca gattgccaat gcgatcggcg gttacacgat
    26821 gtcacaggcg gataccctgc gcaaggtgat gggaaagaaa aaagtcgcgg atatggaaaa
    26881 agaaaaaccg cggtttatgg aaggcgccaa aaaacagaag atcgacgata acaaggccag
    26941 aattatctgg gatcagatgg aaaaattcgc cgaatacggt tttaataaat cccacagcgc
    27001 cgcctatgca atgatcacgt atcagacagc ctatctcaag gcccattatc cggtcgaatt
    27061 tatggccgct ttgttgacga gcgaaaaaga caaccgggac aaaatcatta aacacatgac
    27121 gagctgcaag gacatgggca tcaacatcct gccgcctgac attaatgaat cggaaaaggg
    27181 gttcagtatt tccggtgaga atattcgctt cggtctgacc gccgttaaga atgtcgggga
    27241 ggcggcgatt gaatctgtca tcgacgtgcg agaaacagca aaattcctat cctttattga
    27301 ttttctcaat cgtgtcgatc tgcgtaaagt caacaagcgg gtcattgaaa gcctgatcaa
    27361 atgcggcgca ttcgattcca tgggacataa acgcagtcaa ttaatgcata gttatgaaga
    27421 agcgatggag gttgcccaac gcaaccagaa ggagaaatca agcaaccagt caagtttctt
    27481 tgatcagctg gaatccggct catcatcttt tgaaaacgga ttaagcgctt atgaacttcc
    27541 tgacatgccg gaatgggatc acaaaaagct gctgtccctc gaaagagaag cgctgggttt
    27601 ttatatcacg ggacacccat tgcttccgta tactgatcgt cttaaactgg ttacagacag
    27661 cgattcggaa aatctgctga tccgtcgcga caaggaaacc gtgaccgttg ccggagtggt
    27721 cagcgccata aacgaacgga aaacaaaacg aaacgacacc atgtcgtatg tcactctgga
    27781 agatttgcat ggatcaataa acgctatatt ttttggtgat ttatacaaga catcctataa
    27841 tattcttcat gaagaagaac cggtgattgt taaaggaact cttgatatat ccggcactga
    27901 agaaaaccag aaaatttctt taattgccca agaagtgact cccctggcgg aagccttgaa
    27961 aaatccctac aaacaggttc gttttatggt tgacgccgac aggatgtccg ctgaagatat
    28021 ttcatccttt atctcttcaa ttcggaaatt tcagggaaaa tatgaaagtt atatgcatat
    28081 tatgaacgga aaaagtgaaa cgatcgttta tctgggagat gatttgcgcc tggatattaa
    28141 tgagaggttg aaaaaagagg cggataatat actggggcag ggtgctacaa ttttttcatg
    28201 aggggattga cgtcagatgc cgccatacga ggagagaaca tacagatcgc tcataaagaa
    28261 cgatcatctc atttcttata atgtaaaaat ttccgaaagc gatctgttca tccgatccga
    28321 caccgatctt tcagatcagg ccttccaatc gcttgccaat catcgtcact ctttacaaat
    28381 ctatatccaa aatcattccg atttcagaac gtctcttttg ccggttgatg aagaccatct
    28441 tgcccctgca attgtccggg atatgatcga taaatctaaa atatgcggcg ttggtccgat
    28501 ggcgggcgta gcaggcgcga tatccgaatt tgtcggacgt gaccttttaa gctctacaaa
    28561 aaacatcatt attgaaaatg gcggggatat ttttctgcaa tctcaggata cattgaccgt
    28621 atcaattttt gccggagaat ctcctttaag ccataaagtt catttagaaa tcaagcccga
    28681 agaaacgcct gtgggaattt gcacatcatc cgcttcggtg ggaccttcat taagtttcgg
    28741 aagtgctgat gccgtgtgcg ttctttctcc atcagccacc ctggctgatt cggcagcttc
    28801 tgcgataggc aacagggtaa aaaacaactc tgatattaag tccgctttgg atttcggaat
    28861 gaatatcccc ggtatcaggg gaattgtcat catctgcaaa aaagaaatgg gtatcatagg
    28921 atccgttcga cttctttaga taaaacatca caactcatta taattctata gtcattccgg
    28981 cgttcaaaaa tttattttag aagaagggtt aaagtgattc aatgggatca gagacttttt
    29041 ttaatcgacc ggatggttgt atatttcccc ataccgttat gtttacataa catatcttat
    29101 aagacataat aattttgatt aaatagccgc ataatccaag tctctatatt tatcgtgatt
    29161 gatttagcta tgattattag atgttttatt cggtgtggct tttataatcc atcatcggcg
    29221 agttgctgta taatttttat ttgacggtcc gtcagtctac ggggaacatc aacatttatt
    29281 tttacgaact gatcgccctt caatgctccc tttaatccgg gcacaccgaa tcctttcatc
    29341 cggatcttcg tattattttg agtaccggga gcgatcttga tccgcttggt tgttccgtct
    29401 agggtgggaa catcgatggt cgttccaaga gcggcttgag taaatttaat cgttttttcg
    29461 atatggagat cgttgccgtc gcgggcaaaa attggatgcg gcagaacatt aatattcaaa
    29521 tataagtcac cgctgggacc accattaaag ccggacagac ctttgccggg cagccttaat
    29581 tttttaccgg cgctgattcc tgctggaatt ttgacattaa tatcttctat gcgattttcc
    29641 agttgaaaag aaatcttttt ttccgcccct aaaacggatt cttctaacgt aattgataag
    29701 ttatattcgg catcctgtcc tttttgcggc atatttgcgt aacgtgctcg gtttccgccc
    29761 attgcaccgc cgaacaggcc ggcaaaagga tcgtcatatt ctccttcgta cccccccctt
    29821 tttctgtttg ttctgaaggt tgccctgccc cctttgatac ctccaaagcc gaagcttctg
    29881 agtatttcat caagatcgaa attacggaaa atatcctcct gcgaataccg ctgtcggaat
    29941 tgatcggccg aaccgaattg atcgtattga ttgcgttttt ctggatcgct cagaaccgca
    30001 taagcttcac tgattttttt gaatttttcc tccgccgttt tgttattggg gttcttgtcc
    30061 ggatgccatt taagagccag ttttctatag gctttcttaa tatcatcagc tgatgccttt
    30121 ttttccacac ccaggacctg ataataatct tccgccatat gttttacctc ttattattta
    30181 aatatatttg atgaaagcat caggttttat attaagcatg aattcccatt tgaaaagctt
    30241 ccagattcac ttttattaac ttttccggaa acgacttttt aatgacacgt tcccaaatgg
    30301 atttgtcgat attcatgtgg ttggataagg cgcccagaag gataacattg gccgttttta
    30361 tgttaccagc cttctgggcc agttttgacg cgtcaaaact gatgaccgtt ttgtaattat
    30421 tttgcaaaaa ttcaacggca tcatcaggat aagggctctc acccatgttg acggcaggag
    30481 gatagacttc ctccgtattc acgacaatgg cggcgtcttt tctgagaaac gaaagatatc
    30541 gcagagattc cattttctca aaagatacaa gtgtctgtat gcttccgggc tccgcaagcg
    30601 gagaatagac cttcttcccg taccggacgt ggctcgttac gcatcctccg cgttgggcca
    30661 tgccgtgaac ctcgcttttt tttacgtcaa aaccggcttc catgatgact tcacagataa
    30721 tgtcgcttgc ccgcaggata ccctgcccgc cgactccggc gaataatatg ttttttactt
    30781 cattttcaga catttagata attcctttaa attaacttat agcgttgaat ttacaaacct
    30841 gtgcgcataa tccgcaaccg ttgcataatg ttgcatcaat ataagaaacg ccctttcgct
    30901 tggagccttt tttagttgcc ccttttttcc aggaaatcgg cggacaattc agcccgatgc
    30961 agacccggca tccttcacat ttgtccgggt cgactttcaa ggccggtttg ggttttatat
    31021 ggcttctctt gaatagcgcg cagggtcgtc ttgcaataat cacagaagga cccggttttg
    31081 tcagctcttc ttttatggcg gacgttgttg ctttctgatc gatgggatcg acaaccctga
    31141 catgttcaat gcccagaacg gaaaccagat tttccagctt cacttgcttt gtctctatcc
    31201 ccatcagcgt atagccggtc cccggatgct cctgcatacc ggtcatggcc gtcgtgctat
    31261 tgtccaggat aatgatcaac gcgtcacttt gattgtaagc catatccaaa agcggggtta
    31321 ttcccgaatg cagaaacgtg gaatcgccga tgacgcccac aaccttgccc ttcccttttt
    31381 ccttgagcgc cttgctcatg ccgtgagcca taccgatact ggcgcccatg cagatgcagg
    31441 aatgcagccc ctccaaaggt ttcatgtaag acagcgtgta gcagccgata tccccatgaa
    31501 cataaacctt atgttttttt aaggcataaa acaatccgcg atgcggacaa cccggacaaa
    31561 gattcggcgg tcgcgccgcc aaaggcacct tgtaatcgga cacttttttg atggattgct
    31621 tttcaatcgc atttcggatg actcccggat caaattcgct ggtataagga aaaatttcct
    31681 tgccggtgac tgctatgccc atggctttga tctgttcttc cagaaaagga tcgagctctt
    31741 cgataacgta tagtttttta actttagaag caaaatcacg aatcattttt tccggaagcg
    31801 ggtaaaccat gcccagcttc agataagaat atcccggaaa aacttccttg gcgtaattgt
    31861 aaggcattcc ggcggtaata atgcctgctt ccgcattggt aatttcgacc ttattttccg
    31921 gaaacgtttc agcaaactcc ttgagtgcct gctggcgctt ttccacctcg gctctccgga
    31981 ttctggcaca gtgcggaacc atcacatatt tttccgcgtt atagacaaat cccgtcttgt
    32041 cttcgtatac agccggttct tccagagtga caacggattt tgagtgagat acccgcgtgg
    32101 tggttcgaaa gaaaaccggc gtatcaaacc gcgcgctgat ttcaaaagcc attttaatgt
    32161 agtgtttcgc ttcagcggca tcggccggct ccagcatcgg gattttggcg aaacgggcgt
    32221 aattgcggtt atcctgttca ttctgggagc tgtgcaatga aggatcatcg gcagtaacaa
    32281 tcaccagggc tccgttcgtt ccggtataac tgacggtaaa aacaggatcg gcggcgacgt
    32341 tgacgccaac atgcttcatg accgccattg ccttttctcc ggcgagggcc gcgccgatgg
    32401 caacctcaac ggcaaccttt tcattggtcg accactcggc ataaaccccg tcatatttac
    32461 agaaattttc cagaatttcc gtactgggcg ttcccggata agctgttgca aaccttatgc
    32521 ctgcctcgta tgcgccgcgc gcaatggctt cattacctga catcaatact ttcaataatc
    32581 aaacctccaa atcaactgag agaagaaatt ttatatttga tgaagacggc ttttgaggaa
    32641 tccgctgttt tctccagggc tttcttgtaa ttttccagcg cattctttct atcattcatc
    32701 agttcataaa ttcgggcgac attgctgaac cccactgatt caaatccgat gttgttgccc
    32761 ttttgcgctt tttgaaaata ttcgagcgcg gatttgtaat cttttctggc ttcgtaacaa
    32821 tatccgattg acgacagagc gagaaattta attccggcgt ggtcggaaat ccgggaagaa
    32881 acgaaatttt tgtggtgggc gatggccttg tcatattcac cgaggctgta ataaatattc
    32941 cccagccggt aatgcgccat ccttgcgctc cagctgtgcg gatatttctc gatcagttcc
    33001 tggaaaatcc ggatattgtt cccggtcata tcctgtgttt cacttccgga agcgctcgat
    33061 tgcgtccggg catacatcgc tctggcggag tcctgatagt tgctccagta catatagata
    33121 ccaaaggcaa tcattgatgc cagaatgaca gccgtcacaa tggagtaaaa acgctttttg
    33181 ttttcagaaa tgtaatcctt gaccagttcc acggtgtttt gaaaagcatc cggtccttcc
    33241 aaatcctgtt ttgttaattt ctcagccata tgcctcctct gtgttgatat tatatttttt
    33301 gaccaattcc aaaagcaatt gaggtattct gcgaagcttg ccctcgtgat tggtgcaagc
    33361 gtgaaccgta tagccttccg ccagaagatg tttttgatct tcatcccata tcttggaatt
    33421 gaaaccaacc gtcgctttcc ggatataggc aaattctgtt tccaccgcca aaagctgatc
    33481 gtatgtggca aacttcaggt attcgcagcc gaccttaatc aacggaaggt tcaaattcaa
    33541 ctttgtcaat tcgctgtaag gaacacccag ctgccgcatc agttcgtttc tgccgagttc
    33601 aaaccacttg atgtagttcg tgtgatagac cacccccatg gcatccgtat cggcaaaaag
    33661 caccctgatt tttgttacat tgttaaccga caacgctctt ccagtccttg ataaattttt
    33721 ccatcagcaa atgccccgga gaaatcagaa gattgccggc tttggcgttt atctcattaa
    33781 agctcattcc cggggccctg ttctcttttt tttccgaaga caataccgca aaagggctcg
    33841 gatcgctgac atgggtcctg agatccaacg gtgtcggatg atcgcttaaa acaataatcc
    33901 tatagtggcc gaatgcgcct attttcgtca aaataggccc gacgattttt tcgtcaaaaa
    33961 gttcaatcgc acggattttg ccttcggcat tgccttcgtg tcccatttca tcaggggctt
    34021 caagatggac aaaaacaaag tccatgaaat tcaatacatc cagagccatt tttgctttac
    34081 cttcgtaatt ggtatcaata taaccggttg ccccctgcac cggacaaact ttcaacccgg
    34141 cgatcgttcc aatgccttta agcagatcaa cggcggaaat catgcctccc gatagggaat
    34201 acttctgagt cagagggaca atccgcggct tcttcccctg cccccacagc cagatggcat
    34261 tagcctcttt tttgcccgcc tggcgtcttt tcgcattgac cggatgattt tttaaaaggt
    34321 cggccgcccg cctgataatg gcattgacat cggcggcagc atttccgcgc ggcagatatt
    34381 cagccacggg ctttcccgta atatcatgcg gcggtgttgt gtccggattg gccggggcgt
    34441 tgcgccagac cagcagatga cgatacccca cgccgggaaa gaactccaga tgatcggaac
    34501 cgatgtgccg atggatatcg cgaatgatta tttccgcttc ttcgctggag atgtgcccgg
    34561 cggtaaaatc atccataaac gcttccggac catcctgatc gccgatggta accagattgc
    34621 agcgatacgc gacatcatct ggaccgagat caattcccat gcttgccgct tccagcggtc
    34681 cgcggccggt ataattttcc cggggatcat accccagaac gctcaatgtg gccacatcac
    34741 ttccgggatg aattccttcg ggcaccgtat cgacaaggcc cagtgttcct tctgcggcaa
    34801 tctgatccat gaacggagtg aatgcgcatt ccagcggtgt ttttcctccc agcttatcgg
    34861 tcggataatc cgccatacca tcacccagca atattacgta cttcatatca cctttctgag
    34921 aaatctcttt tttgaataat ttaatttaaa tcgtcctcaa ttctgatcag gatggtttca
    34981 ccctggagga tgtccagttt gcttattctc ttgagtgctt tctgcacatc actttccttg
    35041 gctttgtacg ttgtcatcac aatgggcacc gctccccttt tcactctggc tttctgaatg
    35101 caggcttcaa tgctgatatt gtatttcccg agaatacccg atatcctgga aagcacgccg
    35161 ggacggtcaa ccgcggtaaa acggaaataa tattttgtct ccacatgatc catgggaatc
    35221 agccggattg gcatcatggc cgactcgttg atcgaccgca agggccctcg gccgtttttt
    35281 ccgctcagaa catctcttgc actgtccata acatcgctaa aaaccgcgct ggcggtgggc
    35341 atcatgcccg ctccctgacc gaaaagaaag acggggcctg aggcatcgcc catgatatga
    35401 aaggcattgt aattctgatt cacatttgcc agaagacatt ttgacggaat catcgtcgga
    35461 tgaattctcg cttctaccgc attgtttctg agcattgcga tggccagcaa tttgatccgg
    35521 tagcccagtt ccttcgcaaa cgaaatatct tcacggctga ttttggtgat accttccaga
    35581 tatatatcct tgagattcac tttcctgcca taggccagtg agagaataat ggccattttg
    35641 tgcgcggtat cgatcccttc gatatcaaag gtcggatcgg cttctgcaaa gcccagcttc
    35701 tgcgcctctt tcaaaaccat gtcgaacggc ttgtcgtcat ccgtcatctt gctcaggatg
    35761 tagttgcagg tgccgttcat gatgcccatg acggaaatga ttctgttggc aaccaggctt
    35821 tccttcaagg tcttgatgat tggaatggtt ccgccgacac tcgcttcaaa accgatattg
    35881 accgcatttt tctgagccgc cgggaatatt tcattgccgt gggtcgccag aagcgctttg
    35941 ttggcggtaa ccacatgctt gccgttggca acagcttcga gaacaaacgt gcgcgccggc
    36001 tcgtacccgc ccatcagttc aatgataatc ggaatgtctt tatcgttgat gatctcccgc
    36061 gcatcccgcg tcaagagccc cttatggacc ctcacttcac ggggtgtcgt gatatcaata
    36121 tcggcaattt tctttataac aagcctggcg cccagttttt ttgtaatcag ctcttcattt
    36181 tgccgcaaca gtttgaccac gcccgttccg atggttccga atccgataag accgataggg
    36241 atttctttca ttatatgcac ctgaaattat tttataattt tatataattc ccgttaattg
    36301 ttaatctttt tccgttaatt tcctataaca aatggatcgg ctttgtcaat gaaatatcca
    36361 tcatgaatgc ttaaaataat taatgaatta cccagacatg aagtaaaccg attaaaaacc
    36421 ggatattttg caaaagcccc attacttgac cgtgatcggt ttattttcaa caccggcata
    36481 gaaaacaata atctcggccg cttccttgcc ttcatttttc ccgtaatgtt tcttgttgac
    36541 cagttccaca atggaatctc cggctctgag atgcaaagtt ttgttatcgt ccgccacaac
    36601 cgtcaattct cccttgagca aaacgcccgc gttaatcacc ggatgatgat gcatttccag
    36661 ttttgaaccg gcaggaattc tgatgcgcag aattgtaacc tccggctgac cctggaggta
    36721 ttgcggcagc gtttcgccat cccagctcct gactgttttg gccagttgta ccgtttcaat
    36781 gctatgtaaa cccgccgaac acccggcaaa caaaaaaaat aaaaccgacg gtattaccca
    36841 gatgagcttt ttcaatgttt cctcctctcg ttggattaaa aatcatatag ggggattatt
    36901 ctaattaccg cgcacatggc gggcatacca tcgccgtccc agccagcgtt gatggtgcca
    36961 ggcaaaaaac ggcaagattc ccctttttct caaccagtaa ccttggggaa tctctcttga
    37021 gtcctgtttt ggcatcatta gaatttccgc actgacaacc ccttcgcctt cttccggttt
    37081 acggtgcgca agtatccgcc cttccgcatc aagaataacg gcattgcctt cgtaaaaccc
    37141 tgtatagctc atgggcagtc ccggcatggc acattcaatt tccccgcaat gtgcggcatg
    37201 aatcaccggt acgcctaaaa tacgcgcgtt atcccggagc acctccaacg cattgcgttt
    37261 gttgtccggt tcccagagtt tctgcaaaaa aagcggaaaa ttagtcggta tgctccacca
    37321 gcatgatccg ccgatgacca catccacctt gttttgcagc cggcgggccg tcatcgttcg
    37381 cataaattcc cagcagaccg ccgcgccgac tctgacattt tcgtataagc cgagcacgcc
    37441 gatatccccg ggatcgccgc cttcataaaa ataattttcc cacatggttg gaatatcttt
    37501 atcatgctga ccgacgagcc ggccttgcgc ataacattga taccggttgc ggacttgccc
    37561 gtcgggcaat cggcataaaa atgatccccc gatcaaaacg cggtattttt ttgaaaagcc
    37621 gcaaagaaat gttgccgcct cgccgtccgc agattgaata gcccgggcaa tattcggatt
    37681 ccagctgacg cccgtattga aaaattcagg caaggcaatc cattgtgctc cttcgtttac
    37741 cgcctgcagg gaaaggcgct cacagcgcct tatgttttcc tctgattcgg ccacggcagt
    37801 ggccagttga atggctgcaa ctttaattgt catgggttta ttccttcggc tctttgattg
    37861 attttaattg cgcataaacg tcatcatgta acggcgtagg cacgccataa tcccgaccgg
    37921 ccccgcaaag ataagccgtc agtgcgtcaa tttccgtctg ccggcctttt tcgaaatcca
    37981 gctgcatgga tgttctggaa tgatatccga acccggcaat catcgccatc gctttatcca
    38041 cttcatgctc ctgtataacg acatgatgtg cggcggcgac ggcaaccacc tccatcatca
    38101 tcgcccttgc tttttgccgt aattcaggat tttcccatat ctgcccgtat gttttgccgg
    38161 tggccgatgt cagagacccc agcggacaca ttaacaaata ttttatccag agcacgccgg
    38221 aaatgtcgtc cgttaatacc gcgttgatct ttgcctgaag taaaatatca aggatatatc
    38281 gataatcgtc tgccggatca tgatcggttc cgaacgtcag cttgcacgcg cctccttcct
    38341 gcttaatgat tcccggtccg tcactatggg aaatgatata aacgctgccg cctaagacta
    38401 tcgcatgcgg caggacacgg cgcagccgtt gcgagctgtt aacaccgttg agcaacggaa
    38461 tcaccacggt ttgtctgccg atgcaggaga tgaacgcttc agccgaattt tccaatgaat
    38521 aacttttggt gcaaaagaac accacatcaa acattcccgc ctcatcggga ttgtttgtgg
    38581 ctattctcgg ccggacaaca caatcgcctt cacgcgtaaa aagttgcagg ccgttttttt
    38641 gaatcgcttt taaatgttcc ccgcgggcga taaatatgat ttcatattcg tccgattttt
    38701 cgtactcacg cgccagtttc ccgccgaaat atccgcctac cccgccgata ccaatgatgc
    38761 ctattctcac gcccatcatg cagctccatc cgtttgaaac gccttttcat taaaattatc
    38821 ctgcattgtt attgaagtca agcttcatca tattgatcgt cagcttgctt ttatgatctt
    38881 cgatgtggta ggttactctt cacaaaaaaa ccggatatca acgcaataaa aagcaaactg
    38941 caaataagga gagcttcatg aggattcaat cttttgtcgt tcatttgcgt tttaaatcgg
    39001 caattctttt cgcatcgatc ttatgcatag tcatcagcgg ctgcacgcct ttgctgaacg
    39061 tctcctacaa aaaatccctg cctcccatgg aaggagaaca ggttcttccc ggtttgtccg
    39121 ataacgtcac tgtcaaacgc gacaatctgg gcattcctta cattgaagcg cagagcatgg
    39181 acgatcttat ctttgccatg ggatatatca gcgcttgtga ccgctttaca cagatggaag
    39241 gattcaggct tgtgagccag ggaaggctct ccgaacttct gggcagggcc acgctggaaa
    39301 tggatattta tctgcgggcg ctgaatctca atcaggtcgc ccagaccctt tacctgtcgg
    39361 catcctccga cctgagacga ctgttaaata tctacagcga cggcgtaaac gcgtatctcg
    39421 ccaacgtccc tctgcccacg actctgaagt tggccggaca ccaacccgat ccctggagac
    39481 ccatcgactc catgtatgta tttcttgttc taacgctggg cctgagtcaa aatctccagg
    39541 aagaaatcaa cctgctcaac gtggcgcagg ttgtcgcacc ggaaaagtta gcctggcttt
    39601 ttcccattta ccccgatgaa cccctgccct ttgaagaaat gaaaaaactg aaaggaatca
    39661 atctttcaga ggctgctcaa aatattgaac aactgggcgt catagctaat ttgaccaaca
    39721 cactgcttct tcctgccgcg gcggcttcca ataactgggc ggtctccgga aaactgacca
    39781 aatcagggaa acccatcgtg gccaatgata cgcatctgcc gttgaccatg ccctccatct
    39841 ggcacatgat gcatctgaaa tgtccggaga ttgacggagc cggtattgcc atcgccggtg
    39901 ttcccggcat tgttgccgga tacaacaggc acatcggtat cggcatgacg atggtcatgg
    39961 cggacaatca ggatatcttt ctggaaaagc tgcaacagaa accggacggt ctctattttc
    40021 tttataagaa taaatggttg aaggcaaaca gcagggagga aagcttccgg attaaaggac
    40081 aaaaaaaggc tgttacccga accttttatg aaactctcca cggcgttttg ctcaatgata
    40141 ttttgaccga taagccccgt catgaactgg ttcccatgcc gcttaatcct tcattgggaa
    40201 tcgcgtatag ttgggcaggc aatgaaacgg acaggtccat ggacgttttt ttcaacatca
    40261 tgctcgcaaa atcggtcgat gagattctta aatacacttc tcaggaaacg tccgtcattc
    40321 cactgaacct tgtcatggcg gatcgtaaaa atatcgcctg gcaggttacg ggccgtttcc
    40381 cgttgcggaa aaaaggtcgc ggtctctgtc cctctcccgg ttggacgggt gaatacgatt
    40441 ggaacggatt tctttcaccg tcaaaacatc cgtctttgaa aaaccccgcg aagggctatg
    40501 tgggcaccgc taatcatcgg acagttcctg ctgattttcc ttatattctg tcctcgtcct
    40561 ggtactatcc cgacagaggc gaacgtatcg aacagctgct gaaaaatgca aaaaattaca
    40621 ccctggatga tgcaaaagct atgcagcttg atacctattc caggtttgtc aacgtaatca
    40681 aaacggcgtt gctggaacaa aaaacgctgg acaccatgag ttctgtctgg caaaactctg
    40741 aaaaatcaac ggcggctcat caagcgatgg cactgctcag agattttgac ggaaatatgc
    40801 gcctcgattc cgccggggcg gctttctgcg gcgcttttct gttttcgctg agcgacaatc
    40861 ttttcgctga cgaactggga gggaaaaata cacgtgccta tcaatcactg ctgcatacct
    40921 tcctgctggc ctattccgca ctgcacgacc atttgacgga cagagggaaa agaagccccc
    40981 tctgggatga cgtaaccaca cctcagaaag aagaacgcga cctgattctg gccaaaacgg
    41041 ttctggatgc catcggcatt ctggaaaagc gctgcggaaa aaatcccgac aagtggcaat
    41101 ggggaaaact gcatacctat cattggagga ctgatgccac attgtttgcc gattatatgg
    41161 atttcatcaa caagaccggc gtcaaattcc tctccggtta tgtagacaga ggtccctatc
    41221 cggcgcccgg cgaccacacc acgctgaacg tttcagctta tcatcccggg aaagatttcg
    41281 gaacctggat gattccggcc atgcggatca ttgtagattt cggaagcgat gaacctttcg
    41341 tcggcattaa cagcacaggg cagttggata atccgtcaag cccgcactac gatgacggca
    41401 tcacggcatt ccggttagga cagtatcaga attttccgtt taaggaagaa aatataaaaa
    41461 cacagtacac gaaaatactg acattggtgc ccaagaaatg atttaaaaaa aaagaatatg
    41521 ctttgattgc tttgagccgc cagacacatt attcatgata taccaacttg gctaaagcgc
    41581 ttaacagccc cctaagttca gtcgagtctt ttggactcga ctatttatct gtgtttgaat
    41641 acagccaagt caaaaagact tggctgaaca aagtccacct gaacccgcaa gcttatgatt
    41701 cccatccggg gaatagagaa agaatttacc tgctgacatg gatttttcat aaatcaacac
    41761 agaaccgtct ttgctcaaaa gcggccgctt aggtcgggtc ttcggcggga atttataagg
    41821 attgggaatc agctctttcc ctttcatcac aacaatgtaa tgaatcccct tgccctgttt
    41881 ctttgcttat taccccgttc tggccaaatc cagtttcccc tgccatttcc agattaagac
    41941 ctccttcaga atttgatgtt caactctgtc cagaaacgga tcgcgcgcag ccaaagcgaa
    42001 agcgtcctcc ttggcctcgc tcaaaatacg ggcatcgcga ataatactgg caatgcggaa
    42061 atccggcagc ccggattgtc tcgttcccag aaaatcgccg ggaccgcgga tagcaagatc
    42121 ttcttccgca atggtaaatc cgtccgttgt tgtttccatc acttttaatc ttttgcgcgc
    42181 atctgctgaa catttataat tggccaacag aatgcagctg gagagaatat cgcgccgccc
    42241 taccctgccg cgtaactgat gcagttgcga aagtccgaag cgttcggcat gctcgataac
    42301 cattaatgag gctctcggaa catcgatgcc cacttcgatg accgttgtcg aaacgagaat
    42361 atgaattttg ttctccataa attcccgcat aatttcatct ttttcctttt ccttcatttt
    42421 cccgtggatt aatcccacgc ggtaatccgg aaaaacgtcc tgctgcaaat gcctggacat
    42481 attggtggcg tctttcaaat ccagcgtttc cgactcttcc accagcggat aaacaatgaa
    42541 tgcctgatgc cctttcgcca gttccgcgcg gatggcctga taaactttgt cccgtttgct
    42601 ttccggcaaa acaacagtgt gcacgggctt tttccccgga ggcatttcat tgatcacaga
    42661 gacatccaga tcgccgtata cggtcatcgc cagggttctc ggaatcgggg ttgccgtcat
    42721 gaccagcacg tcagcgttga tgcccttgga tcgcaatgtc gctctctgaa taacgccgaa
    42781 acggtgttgc tcgtcaatga caaccagccc cagtttatga aaatcaacac cttcctgaat
    42841 caaagcgtga gtgccgataa tgatgcccgc ctcgccgttt tgaatccggc gcagggtttc
    42901 gccccgtgat gcattggcca tgctgccggt aagcaaaacc gttttcaatc ccaatggctc
    42961 agcccacaaa cggatatttt caaaatgctg tttggccaga atttccgtcg gcgccattat
    43021 ggccgcctga taagaattct cacaagcggt aatcatggct gccattgaaa caatggtctt
    43081 gccgcttcca acgtctccct gaaggaggcg gttcatcgcc gtgtctttcg acatatcgtt
    43141 gaggatttct tcaatgactc ttttttgagc ggcggttaaa tcgaacttta aactggaata
    43201 aaatttatcc agcattgtgc catcggtttt gaatgaaagg ccttgatcca gaattcttcc
    43261 cgattttttg acagccatgc ccaactgaaa aaagaaaaat tcatcgtaaa tcaaacgccg
    43321 gtgagcctcg gaacggctgg agatgaattg atcaatgtcc tcctgactgc cgggaaaatg
    43381 aacagaccac aacgcttcat ggacattcag caaatgtcgc ttttcacata tactgtccgg
    43441 aatcggagag gcaacatagc gggaataatt catgagtgcg gcattcatga ccttgcgata
    43501 atatttctga taaaggccct ccgtttcgga atacacgggc acgattcttt tgaaattcag
    43561 aagattttct tcatccttct cgtcaagaat ttcataatcc ggatgaatca tctgttttcc
    43621 gacgtaatcc ggtcttacgt cgcccgtaaa aataaccctg gttccttttt tgaaaacacc
    43681 caggagatac gccatctgcc ctttaaacca cttcgcggtc agattggccg tgctgtcgct
    43741 gatcaccgtt tccaggattt tttttcttcc gtaatagcga tattgacagc tgatcacgtt
    43801 gcccacagcc gattggactt tttcggcttc gagacggttg atttttctga tttccctgcg
    43861 gtcttcataa gtccgcggca gaaagaaaag cagatcttcc acggtggcga tttttttatt
    43921 ggcgaagcgg gacgccattt tcgggcccac acctttcaaa tactgaaccg gcagattcag
    43981 tttttccagg gcttctttta aatccgatat cttctcaatg gttttttgat cggcattccg
    44041 ttcgggaacg gatatgttca aaaaatggat gatggttttg agtttttcga cgattttgac
    44101 ggcgttttca atttttcttt tttttaattc cagcttttgc cggtcgtaat cggaaaagat
    44161 ttcccgtaat gctccgatct catgattaat ttcctcacgg ccgaacgatg cggcaaaaga
    44221 atcaagtttc gacaaaagcg ttatgagcgt tctgcccaga tctttgatgt tggaaagatt
    44281 tttgtaatta tcgcggaaag caaagttcag gggttgatca attttttgga gaatttcgtt
    44341 taaggcatcc atttaaatct ccacgccgca aatctgtcaa acctgaatca tttctgatga
    44401 atccggatat ttttgttaac aaatatattt tatttgcgca aggaatgatt gacttaagcg
    44461 ttgaattacg ttaaaaactc accccgaaag aacttccata tgaaatttaa gatcactcag
    44521 aggagctatg acagacaata taacctataa agatgccggc gtggatatcg acatcgccaa
    44581 cgaatttgtg gaacgaatta aaacatccgt caaaacaacg gcgcggaagg aggttgtctc
    44641 cggtatcggc ggattcggcg gccttttccg tctaaattcc gttgacctga aaaatccggt
    44701 gctggtatct tccaccgacg gtgtgggcac caagctgcgt atcgcccacc tgctcgacaa
    44761 acacgatacc atcggcatcg acctcgtcgc gatgtcggta aatgacgtgg ttgtgcaggg
    44821 agccgagccg cttttctttc tcgattatct cgctaccgga aaaatcgagc tcgaaaaaag
    44881 cgtcagcatt atcgagggaa ttacggaagg atgccgacag gcaggctgta cccttctggg
    44941 cggcgaaacc gcggagatgc ccggttttta tgctcccgga gaatacgatc tggctggatt
    45001 ctgtgtcgga gtcgttgaat cggacaaatt aatcaccgga tcaaccatca gtgtcaacga
    45061 ccgtattatc gggctggcgt ccagcggact gcacagcaac ggattttcac tggcgcggaa
    45121 ggttcttctg gaaaaaggaa agctaaacgt caacgataag cttcccggac tggaaaaaac
    45181 cgtcggtctg gaaatgctcg aaccgacccg catttatgtt aaagccctgc tgaatctctt
    45241 taaaaaattc accctcaaag gcctggtcca tgtcaccgga ggcggattct acgaaaacat
    45301 tccgagaatt atcccccagg cctgccgtgc cgtaatcggc aagggcagtt ggcctgtccc
    45361 gccgattttc aacatcattc gtgaaatcgg caacattgat gaaaaagaaa tgttccgcgt
    45421 attcaacatg ggcatcggta tgatgattat cgtttccgac aaagactgtc cggcggtgct
    45481 cgaatatctg gaaattctcg gtgagaaagc gtacccgctc ggcctgattg aaatcaggga
    45541 agataaaaag ccgcaggtca tcatttctga aagctaggga aagcaaatgg cggatgcatt
    45601 gaaactggga attctgattt caggaaacgg gtccaacctg caatccatta ttgatcacat
    45661 cgagcagggg tctcttcggg cggtcatcca aattgtcatc agcaataacc ctgatgctta
    45721 cggcatcatc cgggcaaaaa aacacggcct ccctttttgt gtgctgaaaa acagcgattt
    45781 taaaaataaa gaggaatttg attctgaact tatcaacacg ttgataaaac atgacgtcga
    45841 gctggtaacg ctggccggtt ttatgcgtat catctcgcct cgatttcttc aggcttttcc
    45901 cgggaggatc atgaatatcc atccggccct tcttccttct tttcccggtc tgcacggaca
    45961 gcggcaggcc gtgacgtacg gcgtgaaagt ttccggctgt acggttcatt tcgtggatga
    46021 gggcgtcgat accggcccga tcattataca acgagccgtg ccggtgcgcg atgatgacac
    46081 cgaggacacc ctggcggctc gaattttaaa ggaagaacat aaaatctatc cgcaggcgat
    46141 tcagcttttt gccgacggca aacttgaaat ccggggaaga aaagttttca ttcacccata
    46201 gccgtttttc tccggtctaa tgtctttttt ctgatgatcc ggcaatgacg tttaaggaca
    46261 gaatccatgt atgatctgat tgtcataggc gatgatcttt cttcccatgt agccgcagcg
    46321 ttctgcgcca tcaacggttt gaagacggcc ctcttctccg aaaaaggaac cgccggctca
    46381 tctgttgccg gagatctcac gtttgacggc acgatcacgc cggtgtccgg gtttgccgaa
    46441 aaccagatcg cctatctgtc attaaaaaat ctgggaattt atccggaggt gcgcctgctg
    46501 aatccggcct atcagattat tctcccatcg caaaggatag actttttcca tgacaaagaa
    46561 gaacttctga aagagctcgc cagagagttt cctcatctcg agggcgatct tcgattattc
    46621 tacgatgtag cagagaaaaa ttcaaacgca gccggacaat ggatttcaga acatcccggc
    46681 attcaaccga aaaaaatgaa ggattttttc gattatctca aactgattcc ccgtttcgtc
    46741 gaaagcatgt tcaacaccat ccgtttgaaa cgtctgatgt cgcaaaaccc atccttccgg
    46801 aaagttattc aggctcagca tgcccttctt tctttcaaaa cggatccgtt aaattctttt
    46861 ttttcatcat ggcaatcctg cacgccgttg cgcggcacct tctcctttgc tcagggcaaa
    46921 caggacctgg tcgattcgct gatccgtaaa ataaaagcgt ccgacggtct ttatttatcc
    46981 ggatgcgatt gcctgtcgat ccataaagac aaggtcattg aactgaatta tacggacgat
    47041 aacggcaaca ccgaaaaaat cgaaagccgc catttaatcg tcagcacaaa atggcaaaac
    47101 atcggccaat tgttcgacca caagaaaact ttcagttttg gcgattttct tcggccggca
    47161 aaaacggcgt atttcccctt ttccattttt ctgggcgtca atccggcagg gctgcccgaa
    47221 aagctggcca gacattcggc aattatctgc aatccggcca gggagcttta tgccgacaac
    47281 aacctgatca ttctggaatc gtcggcaaaa gaagagagga tgaaggatcc cgctgcaaaa
    47341 attcttctga gcgccaccgt tttcctcccc gatcatcagg aaatctggtc aaaggataac
    47401 ctgaagacta tttccgaaaa catcatgaat caacttgacc atttcttccc gtttttaaaa
    47461 gaaaacattt tgtttttcga tccggacgaa tccatgaagc tatcgcttaa gctgcgaggc
    47521 accatcatcc cgcgctatca gatcaaaaat tcttttttta cgggtttttc cgctaaaggc
    47581 agtaaaaccc ggtacgccaa catttatctt accggagcgt ccttactggc ggatgccggt
    47641 tttgaagcgg aaattctttc cggactcaat accgcgaatc ttctgatttc acaaaggaag
    47701 taatttatga aagacgtttt ttccatttcc aatctcggca aaagaatgat tgtctctccc
    47761 cttcaaacgg gcaagtttac ccgggacggc aaaagagccc tgtttaacac tcatctggac
    47821 aactatcgtt cgctgaccga taaaaaagga caaccgcttt ccgtcgagtt ggccggcccc
    47881 cgcgaaaaga ttttttttaa tcctgccgac accagagcgg ctatcgttac ctgcggcggc
    47941 ctttgcccgg gtatcaacga cgtgatccgc tcgatcacta cagcgctgta cttcaattat
    48001 cacgtcaaga agattgttgg cgtcaaatac gggctccagg gattaaatcc tcattgcggg
    48061 catccactga tctctctgtc gccggatgtt gtgaaagaca tcagcagctt cggaggcagc
    48121 atcctgtcca catcgcgcgg tccgcaggat gtgcaaatca tcgttgacta tcttcacaga
    48181 ttaaacatca acatcctgtt ctgtatcggc ggtgacggca ccatccgagc ggcggaaaaa
    48241 attacagcgg aaattaccag acgaaatttt aaaattgccg tgatcggcat tcccaaaacc
    48301 atagacaatg atctccatct gattcagaag tcgttcggat ttgataccgc gattgagaaa
    48361 gcggccgatg cgattaaaag cgcccacgtg gaagcccggg gcatggtcaa cggcatcggg
    48421 ctgatcaaaa ttatgggacg ccactccgga cacatcgcag cccacgcagc gctggcacaa
    48481 agcgatgcca attttgtcct cattcccgag gtgccttttg atcttgaagg caaaaacggc
    48541 tttttgggtt ttttaaaaaa acgcatgatt tccagagggc acgctgtcat cattgtcgcc
    48601 gaaggcgccg gtcaggattt gctcggcaca acggaccgct ccgcagaaaa agatgcttca
    48661 ggcaatgctc gtctgctcga tatcggatcg catctgaaaa acgtcatcga aaattatttt
    48721 aaaaaaatca gcatggagat caatctgaaa tatattgagc caagctatct gattcgttcg
    48781 gttccggcaa acgcttcgga tgacatttac tgcaatatgc tgggacaata cgccgtgcac
    48841 gcgggaatgg ccggaaaaac cggaatcctg gtcggtttga tgaaggatga gtatgttcat
    48901 cttccgttgg ccgcagtcgc ctccggcaga aaaatcgatc cccgcgggga tatctggtta
    48961 agggttctgg agacgaccgg ccagccggcc tcgatgaaaa attaatcgaa tattaataat
    49021 atcggatagt taagaaaata ttttcatcgt ctttcagccg gataatttgt tttttattca
    49081 caacaaagga cgattgacat ttatctttca tgattggcga aaaaatgctt gcaaaatact
    49141 gtcttatggt ttagtaacga cgcacatttt ctggaggatt gtaaaaatgt ctcgtgtttg
    49201 tgaagtatgc ggcaaaaagc cgtctgtcgg taatgccgtg agccatgcaa ataataaaaa
    49261 gaaggcggtc tggtatccca acctgcagaa cctgcgttgt gtagatgaaa aaaccggatc
    49321 aataaagaga atcaaggttt gcacacgttg tattcgctca ggatttgtca agaaagctac
    49381 ctgataaaag aaattatttg agattctttt attacatcgg ctgtttcatt gaaaaacagc
    49441 tcatcatcgt caatcaaatc catcgcctgc tcaaacaaac ggttcaattc attattaagc
    49501 aatagatcat tgcagggaag gccgaattta tcttgataaa gatgcattcg gccttggatg
    49561 gtcatcattt gctccatcga ttcttgcgtg atgaacaaca gggcgcttct catcacctct
    49621 cgaatctgat ttacttccag gccgtagagc catcgcaggg tttctgtaaa ttcttccatc
    49681 ctgttttcaa ccgtgtattt tatggcgtcg accatcacct caacagtctt aaacgtgcgg
    49741 agatgcctta cgattaccag atcgtatatc atttcgatga tcaaatggga ctgataaacg
    49801 gcgtcgcgac acgaaatttc tttgcccgca atgccatgta tatcctgtat gactcggata
    49861 agaggattgc ctttccggta ggaatagccc cgggaatgga tatcgaatgc atcgccgtct
    49921 tcggtacaga tatatccgta atgcgaaaga tcatcaatca gcagatgata gatgacatag
    49981 gccgctttcg gatactcaac aggaagggtt tgcagtcttc ttaatttatg cgttctggcg
    50041 gaggttatat ccggatgaat cgccagcaaa tccggggcga tattgtatac aaaaatatcc
    50101 ggttccacat tttttatctc cgcggaaccc agaattttct gcaggaaata tgtatgagtc
    50161 agcatgaaca tatatgataa gcagccatga ccggttatcg gatccggtca tcaactagtt
    50221 ctttcatgtg atatttttta ctcaattcat cgagcaagca attcatctcg cgcagaaaat
    50281 cgggcatgat ggaaatctga atcaccgcca cggcggcatc gatggtcgtt acggtagcca
    50341 ggccctcgta cccctcgata ataaattgca ctgcgacgat attcttgcga tccagctgaa
    50401 aatacttgct catcatccgc tcatggtctc ttggcttcag ttatcgtttg acgtcatcag
    50461 ataaagactg tatgatcaat aaaaaatcaa ggatttttta tgcgccttgt ccgaaataca
    50521 tcgattttgt tattattatt cattgtttcc tgctccccca tccatccctt catcaaaagc
    50581 atatcggaaa gcgatgttgc ctctttcttg cagaacgctc cccgggcgga ggaacatccg
    50641 aacgccgggg ccgatttact gtattcctac agttatgtgg aattctttga agacggccga
    50701 agcatcagca gaaacctgga tcgcattaaa atattcaacg aacgcggccg cagcttcgcc
    50761 tcgaaatcaa tttcgtaccg ggaaggctat cagaaagtga aaattatttt tgccaatacc
    50821 atcaaaccgg acggaaaaat aatcgctctg gacagaaaag atatcatcga ttcttcggag
    50881 tacgccggtt atgaattcta caccgacatc aaagtgaaga agttcaccat gcccgcggtt
    50941 gaggacggct gcattataga aatagcctat gaaattgaaa atttaaaacc cgttttatcc
    51001 atggactact cggaaacgtt tttttgccag aactttttcc ccatcgaaga agacatcatg
    51061 gaaatcgtcc tgccggccgg cacaaaactg caatatgaaa atttcagaac cgacatcaca
    51121 ccccaaatca cttcgttggg aaacaaaata aaatatgttt tcattaacaa gaaacagaag
    51181 gaaattattc ccgaatcccg gatgccctcc ctgctcgatc gcgacacatt tccccagatt
    51241 tctttctgga cgctaagcaa ctgggacatc atttcccagt ggtacatcaa acttttcaaa
    51301 gaacaaatga agtccagcgc cgaactggaa aatttcaccc gcgagcttat tgccgatcaa
    51361 aaaacggatg aggacaaaat caacgccatt tttaatttcg tatcccagaa tatccggtac
    51421 atcgccgtgc tcctgggacc gcatacccac aagccccatc aggcgtttga agtgttcgaa
    51481 aagcgttacg gcgactgcaa agacaaaacc gttctgcttc tgaccatgct taaaattgcc
    51541 agcattcaag gcttgccggc tcttgttccg gccaacggaa aatatttcga tgaaacgatt
    51601 ccatccatga acgcctttaa tcatattatt gccgtcgtcc cgctgaaagg caaatccttc
    51661 tggctggatg ccaccaatga aacgtcatcg tacgattcgc ctccgttcat gctgcccacc
    51721 agaatctttc tgatcaacga tgacggctca taccgtttcg tcacgacccc gccgttaaac
    51781 gacaagaaag attactatca tctggcgatt cgctataaga tcgatacgga aggcaacgcc
    51841 gctattgatt atgtttataa ttttttcggc aaagcggccg aagccatccg gtattccttc
    51901 aaatatgctc ctccggagca acgaaaaaaa ttctttgaga accgcggaat tgaaatcaga
    51961 gaccttgaac tgggaagctt ttcagacacc cgaacgccgt ttacaataaa actttccggc
    52021 atgattagaa atctggcaca ggttctcgac gaggaaacca tggtcctttc caatattatc
    52081 ccgctggatg cataccggga catcacagcc gccagcatcc ggcggtaccc catcagcctg
    52141 acccaatcca tgtatgcaat ggagaataat acctatcagt ttccgccggg ctttaaaata
    52201 aaaaaattgc ctcaaaatta tatatttaaa aagcctttcc gctaccgaaa agaaaaatac
    52261 agttttacag gatccgaatt tcgtgtttat ctggaaacga aaaacgaaga gcagaacatt
    52321 cctctcggca aaatgaatga gtttaaaaaa tatgcttcgg aactgcaaaa gcacgaatca
    52381 gcagtaaaaa acattatttt ttataaaaga taccaaaacc ttgacacccg gaaagacatt
    52441 gatattataa atcataagta acgggaaaag aaacggcaca atgacaacac aaagaaaatt
    52501 gccatcgctg aaatggaaaa tcgaggaagc cggcaataaa aatgacgggg ctctcttaag
    52561 caaagagttt ggcattcatc cgattattgc gcagattctc ctcaacagag gtattcgcga
    52621 tgtcgataag gcccgccatt atcttcaacc gtctttaaac gatctacaca gtcctttcct
    52681 gatgaaagat atgcgccagg gtgttgcccg cctgctgaaa gccattcaca acaatgaaaa
    52741 gatcatgatt tacggcgatt acgacgccga cgggataaca tcggtcgtca ttctttataa
    52801 attcataaga gaaataacgc ccgtcctcga ttattacatt cccgacagaa ttcaggaagg
    52861 ctacggctta aaagaatcgg caatcaacga gttcaaaaaa aacggcgtca gcctgattat
    52921 tacggtcgat tgcggaatct ccgatgtgga acagattgcc tatgcccggt ccctgggcat
    52981 tgatacgatt gttctggacc atcatgaaat atcgggcgga ctgccgcccg cagcagcggc
    53041 catcaacccc aatcgcgacg attgccctta ccctttcaaa ggtcttgccg gcgtgggcat
    53101 cgcgtttaac tttctcatcg ctctgcgcgg cactttgaac aaggaaggct tctggaaaga
    53161 caaccattat ccgaatctca gagaatatct ggatatcgtt acgttgggaa cgattggcga
    53221 catcgctcca ttgacggacg aaaaccgaat cttcaccaaa atcggcctgg agttgatcac
    53281 cgagggccgc cgcccgggca tcaaggcgct caaggaagtc agcggggtgg ataatcaggt
    53341 catcgattct ttcaaggctt ccttttccct gattccccgg atcaacgccg ccggacgcat
    53401 cgcctccccc tccgacgccg ttaaattgct tctgaccgac gatctgtcgg aagcgcggcc
    53461 tctggccgaa aagcttgatt cctacaatcg ccatcgccag ttgatggaga aaaaaatatt
    53521 gagcgacatt ctggaaatga tcggcaaaaa ccgttccctg gaaaaaatga attccctcgt
    53581 tttcgcctcc gataaatggc atccgggggt gattggaatc gtggcgtcgc gtctggttga
    53641 actttttaat cgcccggctt ttgtcatcag tatcatcaac ggcgtgggta agggttcggg
    53701 gcgcagcatt tccggtttca acatctacaa aggcattcag caatgcgaac acttactgct
    53761 ttcattcggc ggccactacc atgccgcggg tatatccatc gaagaagcca atatcgaaaa
    53821 gttttccgct gtcctcgacg aactgaccgg ggacattgcc gaattttctg aaatcgcttc
    53881 tcaaataacg attgatacag aatgtggact tgaggatatc gacccggctt tactggccca
    53941 gatggaggct ctggctccct tcggcagcag gaatcctgaa ccgctgcttc tcgcccacaa
    54001 tgttaaagca tcatctcttg ttgtcgtcgg aaacaatcat ctaaaaatga gcatcaacag
    54061 caatggaacc tcccgcgatt cgatctggtt caacatgggg aaacatctct ccgctctgga
    54121 cggagccagt ctcgatattg tctttacgcc gcagatcaac aattggaacg gttcggcgga
    54181 catccagtta aaaatcaaag atgtcaatat tgtttcatga cctttaacaa gaatcgtctg
    54241 atggttgcat gatgaacgtg gctaccaaag cagcattatt taacgcgctg gtgtttcccg
    54301 gatggggaga gatttatctg aaaaaataca ggcgaggcct tctgatcatc accggcatgg
    54361 ccgccggaat tctatccatt ctacttttag tcgttcagga aaccctggcc gttttgaaag
    54421 ttacaccggt cgacaaatca acgttatcgt tcggcaggct tttccaatta tccgttaagg
    54481 tgatgcattc actgaatccg ttctgcattt tgatcatttt attttttatg atttttctct
    54541 ggctcctctc gattatcgac gcctacagac tgggcaaaga aaccatgttg gaaattagta
    54601 ctgacgctga tcaacaatca gcttctcctg aggcttgatt tcaccggccc taatatattc
    54661 cagagtatta attcccaccg tgcagatttc tcggaataat ttcttgaaat tttcctccca
    54721 ggaagcgctg ccgatttccg gcgatatttt ttcaatccgt aatgtcagga gatcgtcatg
    54781 accggttcgg ctgacaaccg cctgtagcgg aagattattg aatttg
//
LOCUS       DAOOLB010000009        47811 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_526_length_47811_cov_47.093747, whole genome shotgun sequence.
ACCESSION   DAOOLB010000009 DAOOLB010000000
VERSION     DAOOLB010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 47811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 47811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47811
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_526_length_47811_cov_47.093747"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="PLF77_02290"
     CDS             complement(<1..240)
                     /locus_tag="PLF77_02290"
                     /inference="COORDINATES: protein motif:HMM:NF023853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HPC07713.1"
                     /translation="MKKTKKTTAAEREYVIEKEKFVPVSKYFGEDTFNQKTMKERLPK
                     ETFKKLMDAINEDKRLDLKTADVVAEAMKKWALEKG"
     gene            complement(271..2607)
                     /locus_tag="PLF77_02295"
     CDS             complement(271..2607)
                     /locus_tag="PLF77_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPC07714.1"
                     /translation="MTKKESLKVTGQENGIRVESRILEERIQQAVAEGYRHIEITAYG
                     QHGIGGRLWRAGETPIKVDVTGTSGQRLGSMGFANTLIEVFGPVSDDVGWLNAGAKII
                     VHGNATNGVANAMAQGKVFIDGDIGARGMTMTKHNPRFDPPELWVFGKAGDSFAEFMA
                     GGIAVICGVGAENEDDVLGYRPCVGMVGGKIFFRGRQSSYSTQDARLITDLPDEEWRW
                     LTKNLKAFLQDVGKQYLCEELTARRSDWKLLSALKPHEKKGRALSPMTVYRKEAWEKE
                     LGTGGLIGDLTGADQSPIGLITTGDLRRFVPVWANEKYLPPCQAHCPTGIPVQKRWEL
                     IRKGKMKDAVDLALRYTPFPATVCGYLCPNLCMQNCSRKIVSLPSVDVTVLGKASLEA
                     KEPKPLPATGKKIAIIGGGAAGLSVAWQLWMNGHDAVIFESRKHLGGKITQTIPQSRI
                     PDNVVEHELKRLAKNLRHIHLNASLTKEKFRKLKNEFDFIVIAAGAAKPRKLNVPGME
                     KALTALEFLKQSKGNGINVGKKVVIVGAGNVGCDAATEAFRLGAKTVTLIDVQQPASF
                     GTERKHAEAAGAIFLWPRFTKAITARGVELTDGEILPADTVIVAVGDVPDISFLPEEI
                     AVKNGFIAVDEQFRTSDDQIFAVGDVTKPGLLTEAIGAGRIAAGTIDALLRGAGETYD
                     KLPPIDVQRVKTEYFDSRIKAFSDPVSCAVNCASCGACRDCGMCETICPQNAISRKAL
                     NGENYEYVVDAELCMGCGFCAGVCPTGVWEIVDNKPLE"
     gene            complement(2629..4263)
                     /locus_tag="PLF77_02300"
     CDS             complement(2629..4263)
                     /locus_tag="PLF77_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HPC07715.1"
                     /translation="MESAGTVNPSTLSVKDLPWQIMWNKEKCTLCGRCTAVCPVRAIE
                     PGVHRKRALQVPVGLEEKPAQLLSVYFGIDQRTDAAHACVGCGMCTLVCPNGAIMPVR
                     AEETDKLRFHVNQGGEPRRRGGRRNVPDSVLDKIKFIRISMLTDPALDAGRHEFELRT
                     LLGRVLPPEELLKVHRENGWVPPVREIYPLVIGGMSFGALSPNMWEGLQMGVAYLNEE
                     LGMPVRICTGEGGCPPRLLRSRFLKYVILQIASGYFGWDEIIHGLPQMKEDPCAIEIK
                     YGQGAKPGDGGLLMWYKVNKLIAAIRGVPQGVSLPSPPTHQTKYSIEEAVAKMIQSMS
                     MAWGFRVPVYPKISATSTTLAVLNNLTRNPYAAALAIDGEDGGTGAAYNVSMNHMGHP
                     IASNLRDAYLNLVKIGKQNEVPLFAGGGIGKNGNLAANAAALIMLGASGVQTGKYIMQ
                     AAAGCLGSETDRCNICNIGACPKGITSQDSRLYRRLDPEKVAQRLVDVFLSFDTELKK
                     IVAPLGRSTSLPIGMSDALGIADAGAAERLKIKYVL"
     gene            complement(4227..5339)
                     /locus_tag="PLF77_02305"
     CDS             complement(4227..5339)
                     /locus_tag="PLF77_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HPC07716.1"
                     /translation="MTSNDPVSPLAAIRAIDVMREGHDGSGVGLFLTDLGSDFQEFKN
                     EPILSGIFSNEGLKTLDRFMIDHDFMVKYKLSIRPAKNPPAGTPKRDNYVIRIYEYPE
                     EWDGLCRDEIEFRLMMVQLELRRMGEQDESMLVFSFWPDVIIIKEVGDPLAVAEYLGL
                     DRKELMARVILSQGRQNTNYAINIYACHPFFIQGMATVTNGENTAFVPIREFLMSRKF
                     PGYTGYHSDSETFTHILHYMYHQLGMGMEMYKHIITPLKDGELNRHPDGVWLRKLKQS
                     CRPLIIDGPNCVIGCLPDKSMFMVQDSKKLRPGVVGGRPGVFAFSSEMCGLDAALPDR
                     YVNLDFQPMKYETVIVRSERQEIEKWNQLEPLTHPH"
     gene            complement(5604..7166)
                     /locus_tag="PLF77_02310"
     CDS             complement(5604..7166)
                     /locus_tag="PLF77_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPC07717.1"
                     /translation="MTKTSKKSLEERESQLSIHTYEELAALHAIAQILAQPGDLHHQL
                     ALILKEMSSRLGMQRGMISLVDRDNKESWLDVAHDVNIDGLEITYKHGEGITGKVAET
                     GRPMAVANLGQETHFLDRTGARRHLNRAELSFLCVPIIYDFMVEGVLSADKVAVKVEN
                     LDKELALMASVAELMAKAVHIRALEEENKRLRKIIGSTRAPLMEIIGHSKAMQEVFGL
                     VAQVADSNTTVLITGETGTGKELIARAIHKNSVRKNGPMVQVNCAAIPDTLIESELFG
                     HEKGAFTGALTQRRGRFEEANGGTIFLDEVGELSAAAQVKLLRVLQEKRFQPLGSSRV
                     VNVNVRIIAATNRNLEQDILSDRFRADLFYRLNVFPIYLPPLRERGSDVIMLADHFLL
                     KYSKEMGKQVKRISPAAIEVFLSHKWPGNVRELENCIERGVLMTKTDVLDCEHLPPSL
                     QLKDGMHDRKGRGKLSSVVEAQERLLIIDALKETSGNQTKSAKLLGTTKRIIQYKISK
                     MGIDPKNYRKKS"
     gene            complement(7382..8089)
                     /locus_tag="PLF77_02315"
     CDS             complement(7382..8089)
                     /locus_tag="PLF77_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HPC07718.1"
                     /translation="MRAHYFQHVPFEGLGSIESWLKKSGFKITSTRFYESAKLPDLKT
                     IDLLVVMGGPMSVNDEDRFPWLVAEKQFIWDAIAKSKPVLGICLGAQLIASALGAGVY
                     PNKVKEIGWFPIHGIESNGQSVFAFPESISVFHWHGETFDLPSGATLLAKSDACEHQA
                     FQVGKSVIGLQFHLETMPAAAQALVTNCRDELVPAPFIQTEEEILSTTPDQYTAINDL
                     MADILSFLLTQKMTAGL"
     gene            complement(8150..8223)
                     /locus_tag="PLF77_02320"
     tRNA            complement(8150..8223)
                     /locus_tag="PLF77_02320"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8189..8191),aa:Gln,seq:ctg)
     gene            complement(8272..9192)
                     /locus_tag="PLF77_02325"
     CDS             complement(8272..9192)
                     /locus_tag="PLF77_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07719.1"
                     /translation="MKDNLKTGIIMVLLTLILALLPVMSWADEEIVPADPKQGDNAST
                     EFKPVEQDQPEDKVTGEVAASVLSAYIWRGQELTRHSVVIQPSVTAAYKGFAANVWGN
                     LDTKPYSALDVDYSSNFTETDVTLSYLHKFGILQAGIGYIYYGLAGAVQGGADLPDSQ
                     EVFVMLGLDTILAPTLTVYKEFDYYNQWYATLGISHTFALHEKVGLKFAAQASYLRSD
                     DEATYPEFDSASLATTDKFNNLHDGVVSVSLPIAVTGHLTISPVLTYVFPLSDDAKYE
                     MKARGLKGDIDPSHKEGDFLYGGIVVSYAF"
     gene            complement(9214..9417)
                     /locus_tag="PLF77_02330"
     CDS             complement(9214..9417)
                     /locus_tag="PLF77_02330"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPC07720.1"
                     /translation="MSIRIHKAKTNALARCVSECEKAVIMHVLLKTKGDQSEAASLLG
                     IKESFLADKIHQYEIDCARFELK"
     gene            complement(9428..9769)
                     /locus_tag="PLF77_02335"
     CDS             complement(9428..9769)
                     /locus_tag="PLF77_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPC07721.1"
                     /translation="MKLVIAIIQPHKLEAVKRELEAVDVNLMTVTNVLGQGRQKGVTE
                     IYRGAKEAGGLLNKVRLDIAVNEDFVEPTVKAITKGAHTGSVGDGKIFIVELAECIRI
                     SSGERGGLAIG"
     gene            complement(9782..11014)
                     /locus_tag="PLF77_02340"
     CDS             complement(9782..11014)
                     /locus_tag="PLF77_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPC07722.1"
                     /translation="MDSGNTAWVLTASALVLLMTIPGLAFFYGGLVRRKNVLSIMMQC
                     FVIVCVISLQWMLFGYSLAFGPDFHGIIGKLDWMGLAGVGATPNPDYSGGIPHYAFMI
                     FQAMFAIITPALIIGAYAERVKFSAFLVFTLLWATFVYDPLAHWVWGVGGWMRTLGAL
                     DFAGGIVVHVSSGISALVLALMLGKRIGYNHKPIRPHNLPFTVLGAALLWVGWFGFNA
                     GSALAADGLAANAFVTTHIATAAAGITWALIEWWHNGTPTILGAATGAVAGLVAVTPA
                     CGFVNPMNAMFIGMIVAVVCYIAVAVIKGKLGYDDSLDAFGVHGVGGTVGTIATGLFA
                     EKVVNAAGADGLLFGNAHQLYVQSLSLIVTVAFAAIMTFIIFKIVDALIGMRVEENNE
                     VVGLDLTQQSEAAYTVIE"
     gene            11536..14145
                     /locus_tag="PLF77_02345"
     CDS             11536..14145
                     /locus_tag="PLF77_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPC07723.1"
                     /translation="MTPYARVSTGTSSLDDIINGLQMGDNVVLQVDDIEDYKKFVEPF
                     VKTSLASRHRLVYMRFAAHPPLLEANKKIKVYKLNANNGFESFSTQVHNIIKEEGRDV
                     FYVFDCLSDLLMAWATDLMIGNFFVITCPYLFELNTVAYFAILRSSHSFKTVARIRET
                     TQVLLDIHNSNGQLCVHPLKAWKRYTPTMFLPHIMENDKFVPILNSADAASFLSYLTN
                     KNTTGGVRNLDYWDRLFLKASNLLEDPDAVLDRQEMVDSLSRVMIGREKRMLSLVKEY
                     FSLEDLLEIKKRLIGTGFIGGKSVGMLMARNILRKDPAFDWDSELELHDSFYIGSDIF
                     YSYMVQNGWWKLLMAQKTDEGYFEVARELKSRMLHGIFPDEIKEQFQLMLEYFGQSPI
                     IVRSSSLLEDAFGNAFAGKYESYFCPNQGTPETRYKNFEDAVRKIFASTMNEDALTYR
                     VQRGMAHQDEQMALLVQRVSGSHRNRYFFPDIAGVGLSYNTFVWQKGMDPKAGMLRIV
                     FGLGTRAVNRVENDYPRIVALDAPLIKPHAKQEDIRRFSQNEVDVLNLKDNHFQTLPT
                     SDLLDYAIEPHIGMIAVRDAAVDEYYQSLGREAKETWILTFDELLENTPFARTMSDML
                     KKLAQVYRYPVDIEFTANFNAEGKPKINLLQCRPFQTKGHYSRVELPEKIEKSRILLR
                     QEANFMGGSVYQAISRIIYIDPRGYARLTVSEKHEVARLTGKLNKQIGKREKMPTILF
                     GPGRWGTTTPAMGVPVTFSEINNIMAIGEIAYQDGALIPDLSFGTHFFQDMVEMDIFY
                     MAIYPEKEGVFFNTSWMAKQPNILADLMPEDGRFAKVVKVFDMRNQDLRLLSDIVTQK
                     MICFFGDASAIGK"
     gene            14153..17836
                     /locus_tag="PLF77_02350"
     CDS             14153..17836
                     /locus_tag="PLF77_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HPC07724.1"
                     /translation="MISENIRRLVKTVVHFAGRCHIYVGRRPDHVARPSLIFFPVLDG
                     YLCCGFAGLMTCGLKSQPPQTRADLTLVALWKKLKSANVKNPSRLPDVTETVNAMADT
                     VAQLKKEEAQEFLFFHPDYSSDLSVFAADLNRFIAEKENRLDEQTAFLTSAELESVNS
                     LIVMLKDIGWALEKDILANLPKILALTGAGNRADIRPAAFRKYRKLNLALNALDRLEV
                     RGRDSAGIDLTFAPESENDLKAALQRITEQGWLADYEKRTQPGDLLNLSVSVTKNTRG
                     TNAGIVVTFTYKTFSTVGKLGRNVAELRNSIRNDRILRCFADLETSCENVLMHTRWAS
                     VGSITEENCHPVNNHSMIRFACPSTGYPESDVQIHVVLNGDVDNYTPLRQFLETQGEM
                     IARPITTDTKIIPLQIEKFLHSGHTLTEAFRRAVNDLAGSHAIAMTSHLEPGKMFLAL
                     RGSGQSLYIGVSDDQYMFSSELYGLVEVMPRFLKMDGEAGGQICILDQNGPAGVRGIT
                     SYSYDGKILALTENMLGTAEITTRDIDRGDYPHFFLKEISESSLSVKRTLRGKYRMTA
                     TEAALQQVRFNLGEDIVPADIIAGLKKGRIKNIVIIGHGTAAVAGQAVADAFSLYLGS
                     SPLHITARVASELSGFGLKDDLSDTLVIPITQSGTTTDTNRAVAMAKERGAHVISIVN
                     RRQSDITAKSHGVFYTSDGRDIEMSVASSKAFYAQIVAGQVLALFFAQSLRTCTDEDI
                     IACLRNLESAPLLMNQIASDCASVASTVKDAIGKKYWAVTGSGPNKAAADEIRIKLSE
                     LCYKTISTDIVENKKHIDLSAEPLILVCTAGSPESVTADIVKDVAIFKAHKANVIVIA
                     DEGEKRFQGVADAVIAVPVAFPPLPVILNAMAGHLWGYYAACAIDEEAQIFRAFRARL
                     HDILMLKAREQLSVFDLTADVSVKGIINTFYQSFNTRRQQGAFSSLEGKTLSDLMLLL
                     KYAAGKLPLKDMALDFVNDQGIFSPFELLDTTLGTVIDELSRPIDAIRHQAKTVTVGT
                     SRKERPPEGIVFDLLKTLTFDIATLTYRDIMMIRRIQPVIASIPGYTIYDISALDQQG
                     NPTDDSLIAVRVKGGLAAGMKSRADKPAVLMGVKRTIVSTGHAYLGRGKTDNAPILII
                     PLRTQEAGIGNLLLLHVRFDEKLTLQEKIDALGYRYNDIRNLIDEYNLKWDDRFIDRF
                     SLEALFSEPIEIIAGRIKSQLNP"
     gene            complement(17889..18593)
                     /locus_tag="PLF77_02355"
     CDS             complement(17889..18593)
                     /locus_tag="PLF77_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC07725.1"
                     /translation="MARHDIKEARKDNLIDAALRVFAEKGYSDTTMTDIARKAGVSTP
                     VLYEYFKTKEDLLFAIPEKFMEEPIRIMEYVAPYLRGAEAKIRGIVQGYLTVYDENPL
                     YASLVMLELKTNRKFLKAKAYGMVRKVARGLLEVIEEGIKDGTFKKDTDAFLVRSMLL
                     GTIEHLCIRKLLLGEPKNLPQYTDAIVNIILNSIKKESRQFSVNLNINDLLSPNIKTE
                     NIPANKGTSDPKRKKR"
     gene            18863..20356
                     /locus_tag="PLF77_02360"
     CDS             18863..20356
                     /locus_tag="PLF77_02360"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HPC07726.1"
                     /translation="MRTKEQYIKGLRKMKRNLYFNGSKIDRDDEEQLPALNVMGFTFD
                     APHIPEFKDLCTAKSHLTGETINRFCHVHQNSDDLHRKQDMTRALCRTGRMCIQRCMG
                     VDAINGVNAASYEADKQNNGSTEYHKNFLKWLENFQRNDLVGCCAQTDMKGERLKRPA
                     EQTDPDMYLRVVGKKKDGIVVRGCKLHISEASIADEIIVVPTRILGPDEQQFAVSFAV
                     PADYDGVKQVVHIHKIRKRTHFKTGNPDIGYTDSYVIFDDVFVPWERVFLCGEYVHGG
                     ALAMLFALFHRHSYSGCKPAIGDMLLGTASLAAKMNGISKAHHVREILSRIIMVTELG
                     YAAGFTASAKSKPEIYVPGIGQMPYGPGSYIPHSIYANVGRCLTGEAVFDEQEMLCNI
                     AGGLPSTFPYEADLANPELKPLLEKYLKRNPKIPIADQIKFWLLFAEATCSSSTGFMC
                     YGSYHGGGSPIMEQIAITLQYDTKLREHLVNAAAGIEKLDYGRVTKI"
     gene            20460..22484
                     /locus_tag="PLF77_02365"
     CDS             20460..22484
                     /locus_tag="PLF77_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPC07727.1"
                     /translation="MNYRADTLCGLFHHQALRYGDRFVFLTGRFDADGRACDQFSSRT
                     WKQVREEAIALAKGLIALGLQKGKKVVIYSESRPRWIIADQAVQACRAIGVPLYPTVS
                     EEELDYMLEDSESCLAIVSTPEKARRVLKVTAGKAKMPVIMMSPWDDIGQKPEGVYTF
                     PEVFALGEPKVKPDVIEEGIRSVVPEDVASIIYTSGTTGKQKGVILTQSNWIHSMHQC
                     SGSEIMALTAQKDLHLKALVHLPLCHVYGRMSDYHTAGLKMGGELVFAESYQTIARDL
                     REVRPNIINSIPRLYEKTYEIVQSTLSRAKKPYQAIYNWAMSKGRIYVDCMATGKRMP
                     PHQLMLFGLANSLVFDRLKKEMCMDKLVMACSGGGKLSKDICIFYRALGIQLIEGYGL
                     TETTAINNLNAPEIMMEKPPTGFLKVLYDRIMALTLHLMVVRQSQGKSPYANPVMSLL
                     LSLCYNTLVYRLRVKPGFVGRVVPETEMRIAEDGEILIRGPQVFQCYWKREKDTADAF
                     TPDGFFMTGDIGVADEEGFLQITDRKKELFVTSGGKNVAPHPIEVALIERPYIDQACL
                     IGDGRKYLTAFIIPDFESLKRYAKDHGITCASQEDLANHPDIKALITKEVDHVNATLA
                     RYEQIKYFTILGRAFDVASGELTPTMKIKRRVVNEKYQQQIEEMYGKPNQ"
     gene            22521..23393
                     /locus_tag="PLF77_02370"
     CDS             22521..23393
                     /locus_tag="PLF77_02370"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPC07728.1"
                     /translation="MIIDCHYHLEERVFTVERLIGEMQKAGIDKVALMGSIIGPFTEP
                     PRILINLLQRLLENSFSRSIGKAFVSNFTDHGDVRILGKPYPLATDPDNEKVFNAVRK
                     YPDKFLGWIFVNPRGKNNQVEEYEKYKDEKGFIGVKAHPFWHHFAPVELAPVAERLAK
                     TGKPLLIHAGFGEEGNYEALLKKVPDLKLVLAHAGFPCYSDTWKKIKPLKNVYMDLSQ
                     TTYTSGKATRQAVDMFGAERLFFGTDGPFGFHGPDHQYDYGFIKRRIEGMFADVIIRE
                     KLLGGSFAKVVGLS"
     gene            23841..25634
                     /locus_tag="PLF77_02375"
     CDS             23841..25634
                     /locus_tag="PLF77_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HPC07729.1"
                     /translation="MVLHKIKQIVGWLRSFFLENITRWLVFGLLVGIVSGLGASAFYY
                     LLQAGRHLCFHLLAGYAVPAPAGEQVFDVPGILQFHPLIFFLLPALGGLISGLLVYHF
                     APEAEGHGTDAMIDAFHNKKGRIRTRVPVIKGLASIITLSSGGSAGREGPIAQIGAGI
                     GSAVARLLKLSNRERRILLLAGCGGGLSAIFRAPLGGALTAIEILYKEDMETEALVPT
                     VVSSITAYIIFTYFFGNTSIFFFPEYTFSNPRELIFYVLLGLICVPAGIFFIKIFYAV
                     RERIFLPLKIPRYVKPALGGLVVGCIGLVFPQVYGDGWGWIQLAILGKLSIGIMIAIA
                     LAKIVATSFTISSGGSGGVFGPTLFIGGMIGGTVGFSAHYFYPHIVTQPEAFVVVGMA
                     SFFAGVASAPIGTLLMCSEMTRGYGLIAPLMLVSIIAILFTRQHSIYTKQVQSKRESP
                     AHMAEFTVNLFAETQVKDFYKPEKVTPVREITTYGQLKRIFSASNVECFPVYNEDDVL
                     IGAINWDHARSIVFEEGLEDLVIAQDLMAPPQTVTPEDNFYDAFVKFMETNVEELLVV
                     SSDDPNRVLGVLRHDDLIAAYHAELNRRKYE"
     gene            complement(25709..26701)
                     /locus_tag="PLF77_02380"
     CDS             complement(25709..26701)
                     /locus_tag="PLF77_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPC07730.1"
                     /translation="MQKEIQKFLTEHLGKTDFKLESIKKGGSDREFFRVSLPHGDSFI
                     FMRYGDEVVENANWAGINQFMASMDINVPRIILRDIPRHFILIEDLGDVDLWSLRFEP
                     WEERRNHYFQVLAQIHRLHSFDLKSTSPDVQLSESYGPRLYKWEHNYFLENLAWEVCK
                     IKLTSADAAKLNKELDALFESLQKIEPSLIHRDFQSQNIMIKNERPVLIDFQGMRQGC
                     LFYDLGSLLCDPYVTFEEKERDELLDFYYDIIRPAYSRDEFAHNFWAGSVQRLLQALG
                     AYGYLGLKKHKPAFLHHIPNGLENLLTAVDNAGGLKMLNDLATECETIIARRNI"
     gene            complement(26806..27522)
                     /locus_tag="PLF77_02385"
     CDS             complement(26806..27522)
                     /locus_tag="PLF77_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPC07731.1"
                     /translation="MQNKQLNTAFILGAGLGTRLRPLTENTPKPLLEIGGHPMITYAM
                     AHLRAVGIKRFIINTHHCAEKYVEAFPEGNWQGTPITFCHEPVLLDTAGGIKNIEDLI
                     AEDERIIVYNGDIIANMPIELLIRKHFELKTSVTLALRSTGPLLNVSVDKDGFVCDMR
                     HILKNDGVKKCLFAGIYIVEKSFLKRLTTGKIESIVLPLVEMIKENPRSVGGVIIDDG
                     GWYDVGTVAEYEKLSKTDIR"
     gene            complement(27529..28845)
                     /locus_tag="PLF77_02390"
     CDS             complement(27529..28845)
                     /locus_tag="PLF77_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC07732.1"
                     /translation="MSETITETTRNAVRRNPWLWVPSLYLAESIPYTMAMTVSVVLYK
                     DMGLSNTQIAFYTSWLYLPWVIKPLWSPFVDIFRTKRFWILAMQLAIGASFAGVALTL
                     PTTDFVRYSLAVFWIMAFSSATHDIAADGFYMLGLDTPQQAAFVGVRVIFYRIATIAA
                     EGGLVFLAGMLQKYGYPAVGAWSLSFIVVAAVFLAFFIYHIFALPKPAEDISAPWDKS
                     KNFLAEYFRVLTLFFRRKDILIIITFFLLYRFAEAQLTKMVVPFLRDEISKGGLGLST
                     ADIGIIYGTVGVIALMLGGLLGGYVIYRNGLKYWLWPMVFIMHLPDLIFVYLSHYQPQ
                     NLWLVGSAVAVEKFGYGFGFTAYTMYMIMVAQGSYKTVFYAIGTGIMALGMMIPAMGS
                     GWIQDMLGYKNFFIWILITTIPGFIVAALVKIDPEYGKKINKEPRG"
     gene            complement(28976..30295)
                     /locus_tag="PLF77_02395"
     CDS             complement(28976..30295)
                     /locus_tag="PLF77_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HPC07733.1"
                     /translation="MIHYEPEIRVALLQNYSEAHITLKGDFSSAHGRILEGCYTVTVD
                     DGWVLLADSSGKEIVRQKEILLQAGSRSFFTVSDVKIGIDFHWQRTQEQSFRGDLFLS
                     ASRDASFNLINNVLLENYLESVISSEMSAEAPLEFLRAQAITARSWLVSMLAKKQEAR
                     TSTPQRTGNEIVVWQDVNDHDGFDVCADDHCQRYQGITKIISENVHKAIESTRGHFLV
                     YGGKICDARYYKCCGGQTDVFDTAWEDKHYDYLSNISDDVKPHSPVCTEGDARRWLTG
                     RPKAYCNTTDEKLLKQILPAFDRETPDFFRWQVAYEREDLEEIIREKSGIDFGDLKNI
                     EPLERGPSGRISRLKIDGSKRTVMVGKELEIRRWLSPSHLLSSAFVVTVRRNTYGEVI
                     GFVFIGGGWGHGVGLCQIGAAAMASKGFQAEEILTHYFTGAQLKKLY"
     gene            30598..31518
                     /locus_tag="PLF77_02400"
     CDS             30598..31518
                     /locus_tag="PLF77_02400"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC07734.1"
                     /translation="MAHIWPIGGGKGGSGKSFVASSMGRLLAKSGKKTLLIDLDLGAA
                     NLHTMVDVPYPEKCFSDFVSKKIHDLEDTVLQTPFPNLFLISGAHDSLDIANLPYEKK
                     IKTLKAIVKLDYDYIILDLGAGTAFNTLDFFLASRNGIFITTPEPTSIENVYRLMRAI
                     YLRRIRHYFSAADFRTLEKKVREHLGEESFNKPENIVSIIKKEFPEKYALIRKDFNSF
                     RFKLILNQLRKQDNTVLGEQICKIIKKHLGINVEFAGNIAYDEHVHDAICQRVCFLDR
                     YPHTRAANDLRVLNRKMSNAFGEQLMLNYL"
     gene            31540..32193
                     /locus_tag="PLF77_02405"
     CDS             31540..32193
                     /locus_tag="PLF77_02405"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPC07735.1"
                     /translation="MEKDFDQMNYYEILDIGTNATSAEIRGAYNVALQMYQPDSLVSY
                     SFFSKGERDKILSLIDKAYFTLINEAQRAKYDNEMNLRETSVSADQHKAPPQPANIFE
                     FNRRDIASLTRKNNKSALKTKISQSQSIREILSRQKITGADLKAIREELGLAIETIHQ
                     ETKIRLDYLHDIEEDKTEKLPAPVFLKGFVKAYLRSLCIENADDISTAYMNTLPGKN"
     gene            32347..33348
                     /locus_tag="PLF77_02410"
     CDS             32347..33348
                     /locus_tag="PLF77_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HPC07736.1"
                     /translation="MKKSLFYLLLISMLIFFVSACSDSDSDFDDTADAEYLTVQMRAD
                     AGNGEVSLDWQMLPRAETYNIYYMEDDEDDTNQPTSQKMKSEGTKIDGETNHIISAPY
                     VVTGLENGKTYWFALSAKNAEQAESDLSQAIYAIPRANPPLPAPKNVRANAGDKEATV
                     TWDAVTGAAGYIVYYYTSFANYQESPKITSTSYTFTGLTNKQTYLFFVAAVDSNDNNE
                     SGDSSASFVYTTTPSEDPPPSAPRNLRVINRGEGFVDLAWDAPLFHTGALTYNVYFFT
                     AGNITKSTAQKGIEALADTSTRAANLDKEKTHYFVVTAVDNNGESAESGEVSAIPDL"
     gene            33519..34745
                     /gene="gspF"
                     /locus_tag="PLF77_02415"
     CDS             33519..34745
                     /gene="gspF"
                     /locus_tag="PLF77_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HPC07737.1"
                     /translation="MSVFEYIALDEKGRQLKGFIDASGISAARQKLREENIFPVEINQ
                     TETKKESALAGALKINLWERVSANQVAVFTRQLSTLLGAGMPLVPSLSILMQQEKNPL
                     LKKSLAQIREQVNEGKSLTEAMAEFPRIFPSFYLNMVRAGEASGTINLVLERLADFSE
                     NQQALMNKIRSALAYPIIMFIMGSAVIFLLMTFVVPKITGIFTDMNQTLPLVTIVLIK
                     VSNFLKAFWWIILILLFGGLAAIRYMTTGTDSGRRAWDNIRLKIPVMGQINRKIAIAR
                     FCRTLATLLQSGVPLLTAMEIVRNIVNNVIIGDAIHQAARDLEEGKGLSGPLTKSGLF
                     PPLVTEMIAVGEQSGTLETMLNRIAAAYENESQASITVMTSLLEPIMILFMGLVVGFI
                     VVSILLPIFEMNQLVR"
     gene            34774..35226
                     /gene="gspG"
                     /locus_tag="PLF77_02420"
     CDS             34774..35226
                     /gene="gspG"
                     /locus_tag="PLF77_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006751354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HPC07738.1"
                     /translation="MKIFRRKKSRQAGFTLIELMVVIVILGVLAGMIIPRIMGRPDEA
                     RQAKARIQMEAMESALKLYKLDNGSYPSTEQGLKALVEAPTTGDLPKNWRQGGYLEKG
                     KVPKDPWGNDFVYLSPGSHGDFDLSCLGKDGEAGGEGVDQDINNWEVE"
     gene            35189..35740
                     /locus_tag="PLF77_02425"
     CDS             35189..35740
                     /locus_tag="PLF77_02425"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPC07739.1"
                     /translation="MSIRTSTTGKWSKQGFTLVELLLVITLIGIVLVLAVPSTRDVLT
                     GDMLKKTSRQLIGLERKLRTEAVRDQLDYILHLDLSDSVYWITASDMTEIKQAEIKKQ
                     ALHLPEDVRIIDVVDEDNRKITAGEVKIVFKKDNICSPRVIHLAYKEDKMTIVMNPFM
                     GVTDIYDQYVEIPFNTSEGQNRR"
     gene            35760..36122
                     /gene="gspI"
                     /locus_tag="PLF77_02430"
     CDS             35760..36122
                     /gene="gspI"
                     /locus_tag="PLF77_02430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HPC07740.1"
                     /translation="MTEKGFTLMEVMVAMAILAIALVSIFQLQSQSISMATDSRFMTT
                     AALLAQSKMVEVETQSPLSNKTENGDFGPDYPQYAWQLIIGDTQLPQFKKIEVTVTNK
                     LFLRRGTYTLILYKRPDM"
     gene            36125..36793
                     /locus_tag="PLF77_02435"
     CDS             36125..36793
                     /locus_tag="PLF77_02435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HPC07741.1"
                     /translation="MRHCSSRPHLSGGGFTLVEILIAIFILSLVLATVYVSYTGTLKT
                     SHQLEKEGEIYKMARQSLERLAKDLTSLQMSEGSFYLNAEKKKTGRHQFHNIFFWSAA
                     HLTFGELEGEGRPATIGYYVTENPDGKSFSLWRSDATGMKPDETKKTEDGFIICKNIE
                     TFHLTFYDSAGGENDTWETSAGGGMHQGKVPEAVKIELALVNDDDAQKPYKFMTRVFL
                     PVNK"
     assembly_gap    37030..37129
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <37130..38085
                     /gene="gspK"
                     /locus_tag="PLF77_02440"
     CDS             <37130..38085
                     /gene="gspK"
                     /locus_tag="PLF77_02440"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HPC07742.1"
                     /translation="NPRGVALITVILIIVILIAVVIELNRSSRADIYDAANLSDGIKL
                     SYIAKSGFYGAAALLNNSPGAYTSLRDDWAKMETVSLQSNTLFPEGYFIARIEDEAGK
                     IPLNKLVDGEEYNEEIRTMLDRLLAQPEFGLKDRQIDEIIDAIKDWIDTDENTTGYGA
                     ESNYYESLDSPYQAKNAPFDCIEELLMVKGITKEIFYGTKDKPSLAKYVTADSEGLIN
                     INTAPKIVLRSLSDDISSELADRLDEYRMEKSHDVSGNQWYKQVPGMENITINQNLIT
                     VKSTYFKIVSTGKISNMSRTVTGIVATGDQAQIAIITWRQD"
     gene            38085..39599
                     /gene="pilM"
                     /locus_tag="PLF77_02445"
     CDS             38085..39599
                     /gene="pilM"
                     /locus_tag="PLF77_02445"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HPC07743.1"
                     /translation="MPNVLGLEIGRSTIKAVLMARKGLGGGRILDARILDVRDSEGIG
                     PALQKLAEDKNFAGIPCFVSVPSQDVLFRHVRLPFRDDNRIRKTLLFELEPLIPLPIE
                     NVIADYIALPREGLLVGAMPKETIREIIEQVEKNLGDVSVIDTSSSALAVQIPDHKKT
                     SVSIILDIGRNSTTAAFCENESVIHIRSLAFSGEQITLAMAKDFSVDRNRAEQLKISG
                     DYPEAMEKDGEECRGLCSELKNTVEFMKINGVLHNDPAQILLTGGGALFGPLRKEIEK
                     NFTCPVELLNLISARQLEVEKNIMMRMNPSLLNAAAANALRSSAGKKSFNFRQGEFTA
                     RPSHLNFKNHLRWAGILAAVILLLAVVNQILDYSLKMRQIGGIKKDIARIFQKSFPDA
                     PSMVDPVQQLKTKIEEDKKTFGLGEGMPEATTADFLKELSALISPSLEIVFTALTYEN
                     RVISVKGEAKTVDDVTLIKDALAKSKSFKEVTMGPTSLTRDGGKVDFSLRIEVQ"
     gene            39596..40162
                     /gene="gspM"
                     /locus_tag="PLF77_02450"
     CDS             39596..40162
                     /gene="gspM"
                     /locus_tag="PLF77_02450"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HPC07744.1"
                     /translation="MIQELWNKLDATQRKLVAGAAVFVCIALILELAVFPFWEAKKKL
                     DRSISSNQKKLAEVKELSAEFNRLESKVSAMRRVASMRAANFTLFSHLEKKATQAGVR
                     NRIKNMNSSRGIQSASFEESLIDMKLEKVTIKQLVDFLYYAESPADLVRVKKIHIVKM
                     KDAPEYLSVQLQISSIQPSNQIPESGQP"
     gene            40159..40977
                     /gene="gspN"
                     /locus_tag="PLF77_02455"
     CDS             40159..40977
                     /gene="gspN"
                     /locus_tag="PLF77_02455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HPC07745.1"
                     /translation="MKILKNKIFWFTIYGIFITLVFLYLLFPSDIAQSRLEESIASSG
                     LILKTGSLKPSLPLGLKMKNVRLGSGSSPNIYFQGDSLDVQFHPLNFFRDKKNIRLSG
                     KAYGGTFSGNFKTTAAGFHPPEEGMLKFKNIALDRYTLIKALTGKQITGKASGNWTQA
                     AGSGGRENSSATISLFLNKGSYPLAEPFLGLQRIDFDRGEIQARLENRVLKFEKFQIF
                     SPQMDCSLNGDIMLVDEFKNSPVNLTGEIIISGRKVKMNITIEGTIANPSIRYM"
     gene            40983..41909
                     /locus_tag="PLF77_02460"
     CDS             40983..41909
                     /locus_tag="PLF77_02460"
                     /inference="COORDINATES: protein motif:HMM:NF022800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPC07746.1"
                     /translation="MMFPEITAQIKERIGSFKRMPSVSSLDTGVLISVAVVLVITILS
                     YEATDLFYKVVRFPLTRHTAVIKNHHESFVRNNRQHQQLQDYDIITERNLFLTTLRTA
                     GGSESADGPFDADQKSMDFDLKGTIAISSSSGFIIVEERGSKKQKLYRLGDKIGARKL
                     AKITRNTAVLKNGEEEITVKIKETVEDNLLSPAPGSRRNPSPSRNPALSRLGIDGNAK
                     YLQSIMNQAVVRPFMSRGVRQGYIISNIKPSSLYEKAGLQNGDIIVGINNNPLHNAND
                     VMQVLNAMQSGSRLELTVKRRGRTETINYTLE"
     gene            41932..42798
                     /locus_tag="PLF77_02465"
     CDS             41932..42798
                     /locus_tag="PLF77_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC07747.1"
                     /translation="MYAAYFGLKENPFSLSPEPRYLFLSEQHRDALNCLIYGIKEKKG
                     FVLLSGDIGMGKTTVCRSLLASLDDSVETALIFNTAVSDIDLLETILEEYGVDIERES
                     RSKKNYIDRLNSFLLENFSAGKTAVLLIDEAQNLSHAVLEQIRMLSNLETETEKLIQI
                     ILIGQPEINNHLMLPALRQLNERITVRYALKPLSPPEVREYIHHRLKVAHGPGDIRFT
                     GAAYRLIFNFSEGIPRRINTICDRALLIAYTKSITKIDRKIVRLAVHDIGTTYFQKTM
                     SLGRKLLARLTV"
     gene            42832..43611
                     /locus_tag="PLF77_02470"
     CDS             42832..43611
                     /locus_tag="PLF77_02470"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC07748.1"
                     /translation="MSYINDALRKAQQDKKSPYAAYEPLLSPSGKKNTGRRRWLALLG
                     FLALFFCVAVAGLFVLMSGPETKKATAKAPLTIPTAPVIETPVIEAPVQPAVQDIKVE
                     NKVEPVLKTTEKKTQKTEESAALKIRTADSVKLYAEALRKHQEGNLEQAKDLYKKVIE
                     IDPKNIEALNNLGVVYMKKKTYKWAIIRLNDALKIKHDYPDAHYNLACIYAQNNDLSR
                     SLSYLKNAIGFNPQVRQWAEKDKDLEVLADLPEFKKLLEKN"
     gene            43619..45850
                     /gene="gspD"
                     /locus_tag="PLF77_02475"
     CDS             43619..45850
                     /gene="gspD"
                     /locus_tag="PLF77_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HPC07749.1"
                     /translation="MKKSHVLILKAGFLIWVMILLVIISGFAGSALAARVAGGGEEIE
                     TTAMPDAAPEPAIEEEVDPAPEPELMNEPSRQNAEMDAPVMSTDNKDEKRSYKPIKVN
                     PSQKYVTLDFDNVDVSVLVKFVSELTGKNFIIDDKVKGKVTIVSPKKIPVEDVYKVFL
                     SVLEINGLTVVPSGNMIKIIPSVQAREKGLETRTKQDPLLPEDRMVTQIIPLERANPD
                     EVKRILDPLIPKSSSVLSYPPAGMLIITDYLSNIKRIQEIATALDVEGAGDQISYIPL
                     QNASASEVVKSLNTIFTQGPVRGKAATILKVVADSRTNSIILLASGAETENVRKLIAF
                     MDKDVPRGESNIQVYRLQNSVAEDLAKVLTSIVKEGGAAAGATATATGAKTVPTTVVS
                     KNVQIVPDKATNTLVVMAEREDYKIIENIIKQLDIPRPMVYIEALIMEVNANKDFKLG
                     VEWRGITEHGSVDGNPTAAFAGSGGAGPSGPGSYNIMDGLLTTTGMGVFPSGFSMGLV
                     GAGIKIGGVTFPSIGAVVQAYKTDSEVSILSTPQIMTLDNEEAEINVGSNVPYVTRQD
                     QTNVGTGTNYTNYSSYEYRDVGVILKITPNINQENFIRLKIDQQVTKVQSGADEARPT
                     TLKRTAKTTVIVKDNETVVIGGLVGDSTDDSTYKFPLLGDIPLLGWLFKTKTSSREKT
                     NLYVFLTPHIVRTQADAAKIYQEKRETMGEVVEGVIKLNEKADAKAPQVRPPVENETI
                     SNP"
     gene            45871..47664
                     /gene="gspE"
                     /locus_tag="PLF77_02480"
     CDS             45871..47664
                     /gene="gspE"
                     /locus_tag="PLF77_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HPC07750.1"
                     /translation="MSKIHTALKESAASAHGDASSGKSVFSSLDEKLIAAGVAPDTLS
                     EIRTAPANNGGGFFDQLIKKKVIPEIHLLKILSEHFGLSFWQQLPMESIDIDFTQRIP
                     IQYLKKHKIIPLITAENPVIALNDPSNFQPVDDLRQLLKNPNLSVVLSLQDAIMSAIN
                     MAYDMSRSSAKDYFEEMDQSSTDDLISKIDETADLLDEASDAPIIKLVNLILSGAIKD
                     RASDIHVEPYSANLKIRYRIDGILYDTLSLPRRIQSPLISRIKIMAKLNIAEKRLPQD
                     GRIEIKIADRLVDIRVSVIPTAFGERVVLRLLDKTANILMLSDLGMHDERIKLLNKLI
                     KSPYGIILVTGPTGSGKTTTLYAALSTINRPEINIITIEDPIEYQMDGVGQIQVNPKI
                     DLTFAAGLRSIVRQDPDVILIGEIRDRETAEIAIQSSLTGHLVFSTLHTNDAASAVTR
                     LIDMGIEPFLVTSSIVAIIAQRLVRVLCPHCKEVYEPDEESLANLGLSRSVLKNNTFY
                     RKKGCNLCMQTGFRGRSAIFEILTVDDEIKRLVLKTSDANQINELAIKRGMITLQQDG
                     IDKVLAGITTIEEVLRVTRTLSRTDDIVIEV"
BASE COUNT        12539 a        12120 c        11495 g        11557 t
ORIGIN      
        1 ccctttttcc agagcccatt tcttcatggc ctcagccacg acatcggctg tctttaaatc
       61 cagccgcttg tcttcgttga tggcgtccat cagttttttg aaggtttctt tcggcagacg
      121 ctctttcatc gttttttggt tgaaggtatc ctcgccgaaa tactttgaaa ccggaacgaa
      181 tttctccttt tcaataacgt attcccgctc cgctgcagtg gtcttttttg tttttttcat
      241 agtctttctt atcctcgcta ttggattttt ttactcgagt ggtttattgt ccacgatttc
      301 ccagacgccg gtcggacaaa cgccggcgca aaaaccgcac cccatgcaaa gttccgcatc
      361 aacaacatac tcataatttt caccgtttag ggctttgcgc gaaatggcat tctgcggaca
      421 gatggtctcg cacataccgc agtcacggca ggcgccgcag gacgcgcagt tcacggcgca
      481 ggaaacggga tcggaaaaag ccttgatgcg cgaatcgaaa tattctgtct tcacccgttg
      541 aacatcgatc ggcggcagtt tgtcatatgt ttcaccggcg cctctgagca atgcgtcaat
      601 tgttccggcg gcgattcttc ccgcgccgat ggcttcagtg agcaaaccgg gcttggtgac
      661 atcgccgacg gcaaatatct gatcgtcgct tgttctgaat tgttcgtcca ccgcgatgaa
      721 cccgttcttg acggctattt cttccggcaa aaacgatata tccggaacgt cgccgacagc
      781 cacgatgacc gtatccgcag gcaggatttc cccgtcggtc agttccacgc ctctggccgt
      841 gatggccttg gtaaagcggg gccagaggaa gatcgcaccc gccgcttcgg catgtttccg
      901 ttccgtgccg aaggatgccg gctgctgaac gtcaatcagc gtaacggttt tggcgcccag
      961 gcgaaaagct tccgtcgcgg cgtcacatcc gacattgccg gcgccaacga tgacgacttt
     1021 ctttccgaca ttgatgccgt tgcctttgct ctgtttcaga aactcgagcg cggtcaatgc
     1081 tttttccatt ccggggacat ttagtttccg gggttttgcc gctccggcgg caatgacgat
     1141 gaagtcgaat tcattcttca attttctaaa cttctctttc gtcaaagacg cgttcagatg
     1201 aatgtgacgc agatttttcg ccagccgttt gagctcatgt tccacaacat tatcgggaat
     1261 acggctctgc ggaatggttt gggtaatttt accgcccaga tgttttctgc tctcgaaaat
     1321 caccgcgtca tgaccgttca tccataactg ccaggccacg gaaagtcccg cggcgccgcc
     1381 gccgatgatc gctatttttt taccggtggc cggcagtggt ttcggttctt ttgcttccag
     1441 agacgctttc cccagaacgg ttacatccac cgacggcagg ctgacgattt ttcgcgaaca
     1501 gttctgcatg caaagattgg ggcaaagata accgcaaacc gtcgccggga aaggcgtata
     1561 ccgcagggca agatccaccg cgtctttcat ttttcctttg cggatcaatt cccatcgttt
     1621 ttgaacggga atgcccgtcg gacagtgcgc ctgacagggc ggcagatatt tttcattggc
     1681 ccagacggga acaaaacggc gtaaatcacc ggtcgtgatc agaccgatcg gggactggtc
     1741 ggcgccggtc aagtcaccga tcagaccgcc ggtgcccagt tccttttccc atgcctcttt
     1801 ccggtaaacc gtcatcggac tcagggcacg ccctttcttc tcgtggggtt tcaaagcgga
     1861 cagaagcttc cagtccgaac gacgggcggt cagttcttca cataagtatt gtttgccgac
     1921 atcctgcaga aaggctttca gattttttgt cagccaccgc cattcctcat ccggaagatc
     1981 ggtaatgagt cgcgcatcct gggtgctgta agagctttga cggccgcgga agaaaatctt
     2041 gcctcccacc atccccacgc agggacggta acccaaaacg tcatcctcat tttcagcgcc
     2101 gacaccgcag atgaccgcga tgccgccggc catgaattcc gcgaacgaat cgccggcttt
     2161 tccgaacacc cagagttccg gcggatcaaa acgcggattg tgtttggtca tggtcatgcc
     2221 ccgggcgccg atatcgccgt caataaaaac tttcccctgg gccatggcgt tggccacccc
     2281 gttggtggca ttaccgtgga caatgatttt cgctccggca ttgagccagc ccacgtcatc
     2341 ggaaaccgga ccgaacacct cgatgagcgt gttggcaaac cccatggaac cgagacgctg
     2401 tcccgatgtt cccgtcacat ccaccttgat tggcgtttct ccggcccgcc agagacggcc
     2461 gccgatacca tgctggccgt aagccgttat ctcaatatga cgatagccct cggctacagc
     2521 ctgctgaatc cgctcttcga gaattcgcga ttcaacacgg attccgtttt cctggccggt
     2581 tacttttaac gattctttct ttgtcataat ccctgcactg ttttcattct acagaacgta
     2641 ttttattttt aatctctcag ccgcgccggc atcggcaatc cccagcgcat cggacatgcc
     2701 gatcggcaga gatgtcgaac gccccagcgg tgcgacgatt tttttcaact ccgtatcaaa
     2761 gctcaaaaat acatcgacga gccgttgagc gaccttttcg ggatccagtc tccgatagag
     2821 tcgcgaatcc tgcgaggtaa tgcctttcgg acaggcgccg atattgcaaa tattacagcg
     2881 gtccgtctcg gaccccagac aaccggccgc cgcctgcatg atatattttc cggtttgaac
     2941 accgctggca cccagcatga tcagggcggc ggcattggcc gcgagattgc cgttcttgcc
     3001 gatgccgcct ccggcaaaca gagggacttc attttgcttg ccgatcttga ccaaattcag
     3061 atacgcgtcg cgcaaattgc tggcgatcgg atgccccata tgattcatgg agacattata
     3121 agcagcgcct gttccgccgt cttctccgtc aatggccagc gcggcggcat aagggttcct
     3181 ggtcagatta ttcagcacgg caagcgtcgt gctggtcgcg gatattttcg gatagaccgg
     3241 cacgcggaaa ccccaggcca tggacatgga ctgaatcatc ttggccacgg cttcctcgat
     3301 cgagtacttg gtctgatgtg tcggcgggct gggcaggctc acgccctgcg gtacgccgcg
     3361 gatggcggcg atcagtttat tgaccttgta ccacatcagc aatccgccgt cgccgggctt
     3421 ggcgccctgg ccgtatttta tttcaatcgc gcagggatct tctttcatct gcggaagacc
     3481 atgaatgatt tcatcccacc cgaaatagcc actggcaatt tgcagaatga catatttcaa
     3541 aaaacgggac ctcaggagtc gcggagggca tcccccctct ccggtgcaga tgcgcacggg
     3601 catccccagt tcttcattaa gatacgccac ccccatctgc aggccttccc acatattcgg
     3661 ggacaatgcc ccgaaagaca tcccgccgat aaccaacgga tatatttcac gcaccggcgg
     3721 cacccatccg ttttcacgat ggactttcaa aagctcctcc ggcggaagca ctctgcccag
     3781 cagggtcctg agttcaaatt catgacgtcc cgcatccagc gccggatcgg tcagcataga
     3841 aatccggata aattttattt tatccagaac gctgtccgga acattgcgcc gtccgccgcg
     3901 cctgcggggc tcgccgccct ggttgacgtg aaaccgcagt ttgtctgttt cttccgcacg
     3961 caccggcatg atggcgccgt tgggacaaac cagagtgcac atgccgcaac cgacgcaggc
     4021 atgcgccgca tcggtgcgct gatcaatccc gaaataaacg gacaacaact gggcgggttt
     4081 ttcttccaga ccgacaggaa cctgcagcgc gcgtttgcga tgcacgcccg gttcgatcgc
     4141 acgcaccgga cacaccgcgg tgcaccggcc gcacagggtg catttctctt tgttccacat
     4201 gatctgccag ggcagatctt tgacgcttaa tgtggatggg ttaacggttc cagctgattc
     4261 catttttcga tctcctgccg ttcgctgcga acgatcactg tttcatattt catgggttga
     4321 aaatccaggt taacatatct gtccggaagg gccgcgtcga ggccgcacat ttccgaagaa
     4381 aacgcgaaca ccccgggacg accgcccacc acgccaggac gaagtttttt cgaatcctgc
     4441 accatgaaca tcgatttatc cggcagacat ccgatgacgc aattgggacc gtcaataatc
     4501 agcgggcggc aggactgttt caattttctg agccacacgc cgtcgggatg acggttcagt
     4561 tccccgtcct taagcggtgt aatgatatgc ttgtacattt ccatgcccat acccaattga
     4621 tgatacatgt aatgcagaat atgcgtgaac gtttccgagt ccgaatgata accggtataa
     4681 cccgggaatt tgcgggacat cagaaattcc cgtatcggaa caaaagcggt gttttctcca
     4741 tttgtcacag tcgccatccc ctgaataaaa aacggatggc aggcatagat attgatcgca
     4801 tagtttgtat tctgtcgtcc ctggctcagg ataacgcggg ccatcagctc tttgcggtcc
     4861 agtccgagat attcggcaac agcgagcgga tctccaacct ctttgataat gatcacatcg
     4921 ggccagaaac tgaacaccag catcgattcg tcctgctcgc ccatccgccg caattccaat
     4981 tgaaccatca tcagccgaaa ttcgatttcg tcccggcata atccgtccca ctcctcggga
     5041 tattcataaa tccggataac gtaattatcg cgtttgggcg ttcccgcggg aggattcttt
     5101 gccggtctga tggaaagttt gtatttcacc ataaaatcat gatcgatcat gaatcggtcg
     5161 agcgttttga gtccttcatt ggaaaaaatc ccggaaagaa tcggctcgtt tttgaattcc
     5221 tggaaatcgc ttcccagatc cgtcagaaat aacccgacac ccgaaccgtc atgcccttcc
     5281 ctcatcacat cgatggcgcg aatggctgcc agcggggaaa ccggatcatt acttgtcatg
     5341 ccaaacaggc gacacatatt ttctttcctc cttggtacaa aaagagcaca cttggaccgc
     5401 cttggctaac tgctaaagtc gtgccatcaa tgttgaaaat taaaatgtcc tgaattaatc
     5461 aacttttagg tattttttat ggagaattgc cgtacagata aaacaatgga agtgacattt
     5521 ttgttcttag atagaaaagt gaaacatttt tgttcgattt gcaccgaaaa atgtagagag
     5581 gtcatggcag gggaaggcca tctttagctt tttttacggt aatttttggg atcaatcccc
     5641 atcttggaaa ttttgtactg gataatccgc ttggtcgtac ccaacagttt ggccgatttg
     5701 gtctgattac cgcttgtttc tttaagcgcg tcaatgatca gcaatctctc ctgagcctcc
     5761 acaacggaag acagtttgcc gcgacctttg cgatcatgca ttccgtcttt caattgcagg
     5821 gacgggggca gatgctcgca atccagaaca tccgtcttgg tcatcagaac accccgttca
     5881 atacagtttt ccagttcgcg gacattgccc ggccatttat gggaaagaaa aacctcgata
     5941 gccgcagggg aaatccgttt cacctgtttg cccatctctt tactgtactt cagaagaaag
     6001 tggtcggcca gcataatcac atcgctgccg cgctcgcgaa gaggaggcaa ataaatggga
     6061 aatacattca gccggtaaaa caggtccgcc cggaagcggt cggaaaggat atcctgctcc
     6121 agattgcggt tggtcgcggc aataatccgg acattgacat taaccacacg ggacgacccc
     6181 agcggttgaa aacgtttttc ctgcaaaacc cgcagcagtt tgacctgggc ggcggctgaa
     6241 agttccccga cctcgtcaag aaagattgtg ccgccgttgg cttcctcaaa gcgcccgcgg
     6301 cgctgcgtca aggctccggt gaacgcccct ttttcatgac cgaacaattc actttcaatc
     6361 agggtatccg gaatagccgc gcaattgacc tggaccatgg gaccgttctt acgcactgag
     6421 tttttatgaa tggcacgggc gattaattcc ttgcccgttc ccgtttctcc ggtgatcaaa
     6481 acggtggtgt tggaatcggc cacctgcgcc accagcccga agacttcctg catcgccttg
     6541 gaatgtccga taatctccat caaaggcgct cgggtgctgc cgatgatttt acgcaaacgt
     6601 ttgttttcct cctcaagagc gcggatatga acggccttgg ccatcagttc ggcgacggat
     6661 gccatcaagg cgagttcctt atccagattt tcgactttga cggccacttt atcggccgag
     6721 agaaccccct ccaccatgaa atcatatata atcggcacgc ataaaaagga aagctccgcg
     6781 cgattcaaat gacggcgggc accggtgcgg tccaggaaat gcgtttcctg acccagattg
     6841 gccaccgcca tgggccggcc ggtttctgca acttttccgg taatgccttc gccatgttta
     6901 taggttatct caaggccatc tatattcaca tcatgggcca catccagcca cgattcctta
     6961 ttatcgcggt caacgagcga tatcatcccg cgctgcatcc cgagacgaga actcatttcc
     7021 tttaaaatca gtgcaagctg atggtgtaaa tctccgggtt gggctaaaat ttgcgctatc
     7081 gcatgcaggg ctgccaattc ctcataggta tgaatactca attgactttc tctctcttcc
     7141 aaagattttt ttgacgtttt agtcatcttt atttcgccag caatttattt tgttacaaaa
     7201 ttgttcaaat ttcaaccaat gttttttctt taaaatctct ctatcgcaaa taatgcgcca
     7261 aatcattaca cctttgtaaa aaaatgcaga aagaatgcga aattatcgcc gctgctgaac
     7321 cgggcaagat cataaatacg taaagatatt tattatgagt atttgataca ggacaactcg
     7381 attacagtcc tgccgtcatt ttttgtgtaa gcagaaaaga taaaatgtcg gccatcagat
     7441 cattgatggc tgtgtattga tcaggagttg tcgacagtat ttcttcttcg gtctgaatga
     7501 agggcgccgg gaccaattca tcgcggcagt tggtaacaag cgcctgtgct gctgcgggca
     7561 tcgtttccaa gtgaaactga agaccgataa ccgacttgcc gacctgaaac gcctgatgtt
     7621 cgcaggcgtc gctttttgcc agaagcgttg ctcccgacgg caaatcaaac gtctccccgt
     7681 gccagtggaa tacgctgatg gactccggaa acgcaaaaac ggattggccg tttgattcaa
     7741 tcccatggat cggaaaccag ccgatctctt ttactttatt gggatacact ccggcgccca
     7801 acgcgctggc aataagctgc gcgcccaggc agattcccag caccggttta cttttagcga
     7861 tggcatccca gatgaattgt ttttcggcaa ccagccaggg gaatcgatct tcgtcgttga
     7921 cgctcatcgg accgcccatc actacaagaa gatctatcgt tttaagatcg gggagtttgg
     7981 ccgattcata gaatctggtg ctggttattt taaaaccgga ttttttcagc caggactcga
     8041 tgctgcccag cccttcgaaa ggaacatgct gaaagtagtg cgcgcgcatg aaagtttttt
     8101 tctccgattg tcattgaaat ttttagtatc tcagtaaatt tgctgcaaat ggctgatggg
     8161 caaggactcg aacctcgatt cacagggcca gaacctgttg tcctaccttt agacgaccca
     8221 tcagcaaaat ccattcaaag atcatctacc aaaacgcatc ggcatttaaa tttagaatgc
     8281 gtaactcact acaatgccgc catacagaaa atcgccttcc ttatgggagg gatcgatgtc
     8341 tcctttcagt ccgcgggctt tcatctcgta tttcgcatca tcggaaagcg gaaagacgta
     8401 ggtcagcacg ggactgattg tcagatggcc ggttacggca atcggcagag aaacggagac
     8461 aacaccgtcg tgcaaattat taaatttatc ggtcgtggcc agtgaagcgc tgtcaaattc
     8521 cggatacgtt gcctcatcgt cgcttctcaa ataacttgct tgagcagcaa atttcagacc
     8581 aactttttca tgcaacgcaa aagtatgaga aattcccagc gtagcatacc actgattgta
     8641 gtaatcgaac tctttataaa cagtcagcgt cggagcaaga atcgtgtcaa gtccaagcat
     8701 tacaaacact tcctgtgaat ccggaagatc ggcgccgccc tgaacggctc ctgccaggcc
     8761 gtaatatatg taacctatgc ccgcctgcaa gatcccgaat ttatgcaaat aggacagggt
     8821 aacatccgtc tcggtgaagt tggatgaata atccacatcg agagcagagt acggttttgt
     8881 atcaagattt ccccagacat tggcggcaaa gcctttataa gcggcggtaa cggagggttg
     8941 aatgaccaca ctgtgacggg tcaattcctg tccgcgccag atataggcgc tcaaaacgct
     9001 tgccgcaacc tcgccggtca ctttatcttc cggctgatct tgctccacag gtttgaattc
     9061 cgttgatgcg ttatctccct gtttcggatc ggcaggaact atttcttcat ccgcccaact
     9121 catcacgggc agcaatgcca ggatcagagt cagcaaaacc atgattattc cagtttttaa
     9181 attgtctttc atctttttca ttctccttta caattatttt aattcgaatc gggcgcaatc
     9241 aatttcatat tgatgaattt tatccgccag aaaactttcc ttaattccca aaagtgaagc
     9301 cgcttccgat tgatcgcctt tcgttttgag cagaacgtgc atgatcaccg ctttttcgca
     9361 ttcggaaacg caacgggcca gggcgtttgt tttggcttta tggatgcgta tgctcatagc
     9421 ggccgcctca accgatggct aagccaccgc gttcaccgct gctgattctt atgcactcgg
     9481 caagttcgac gataaagatc tttccgtcgc cgaccgagcc ggtatgggcc cctttcgtga
     9541 tcgctttaac ggtcggttcg acaaaatctt cattgaccgc gatatccagc ctgactttat
     9601 tgagcaggcc tccggcttcc ttcgcgccgc ggtaaatttc cgtgacgcct ttttgccggc
     9661 cctgtcccag aacattggtg acggtcatca gattgacgtc cacggcctcc agttctcttt
     9721 tcaccgcttc cagtttgtgc ggctgaataa tggctatgac taatttcata atgcattcct
     9781 cctattcgat cacggtgtaa gcggcttcgc tttgttgcgt taaatccaga ccaacgactt
     9841 cattattctc ttccacacgc atgccgatga gcgcatccac gatcttgaag atgatgaagg
     9901 tcatgatcgc ggcaaaggcg acggtgacaa tcagcgacag agactgaacg taaagctgat
     9961 gggcattgcc gaaaagcaga ccgtcggctc cggcggcgtt gacaactttt tccgcgaaca
    10021 atccggtggc gatggttccg acggttccgc ccacgccgtg cacgccgaag gcatccagag
    10081 agtcatcgta gccgagtttg cccttgatga ccgcaaccgc gatatagcag acgacggcga
    10141 cgatcatgcc gataaacatg gcattcatcg gattgacaaa accgcaggcg ggcgtgacgg
    10201 caacgagacc ggccacggcc cctgttgccg cgcccaaaat ggtcggcgtg ccgttgtgcc
    10261 accattcaat caaagcccag gtgatgccgg cggcagccgt ggcgatatgt gtggtcacaa
    10321 aagcattggc ggccaggcca tcggcggcca gggcgctgcc cgcgttgaag ccgaaccagc
    10381 caacccacaa tagagcggcg cccagaacgg taaagggcag attatgcggc cgaatgggtt
    10441 tatggttgta gccgattctt ttgcccagca ttaaagccag cactaaagca gaaattcccg
    10501 aactgacatg cacgacaatt cccccggcaa aatccagggc gcccagcgtt ctcatccagc
    10561 cgccgacacc ccagacccag tgggccagcg gatcataaac aaaggtcgcc cataataagg
    10621 taaagaccag gaaggcggaa aatttcacgc gttccgcgta agcgccgatg attaatgccg
    10681 gcgtaatgat cgcgaacatg gcctgaaaaa tcataaaggc gtaatgagga atgccgccgg
    10741 aataatccgg attcggcgtt gcgcccactc cggccagccc catccagtcc agtttaccga
    10801 tgatgccgtg aaaatccggg ccgaatgcca gcgaataacc gaacagcatc cactgcagac
    10861 tgattacaca aacaatgaca aaacattgca tcataatgga gaggacgttc ttgcggcgaa
    10921 ccagtcctcc gtaaaaaaat gccagccccg gaatggtcat taacaaaacc agggcgctgg
    10981 ctgtcagaac ccacgctgta tttccagaat ccataatcaa tacctcctga tgaatttatc
    11041 cttcgtctgt attatggcaa ggacaatgcc aaaatgtttt tattgtgata agttattggt
    11101 attatttatt aatacgaaaa ttttgaggct gataaattga tacaatactg tataaatcat
    11161 ggtttttgag tcctgaaatg ttgacacttc tcccgtttaa aaacaatccg gcttctcaaa
    11221 aaacgtttca gaaatgtttc ttccgcaaaa ttgccgtcca aagattttta atttctacct
    11281 ccccctcccg aatgcagttc gttccatgca agaagaaaaa tattctggca caaaaattga
    11341 ttcatctatc aggaagcaga aactgatttt tcatcaaaca ccgccggatg caccgataaa
    11401 cgtcattgtg ctgatgaaaa tacttgaaat tattattctt ttgaaaagag gcgccgttta
    11461 ggacaagaag aaataaaaat ggaacgatta tttatggtac aaatttgttc cgaataatgg
    11521 atgagggaaa gatttatgac accatatgca cgcgtcagca caggaacgag cagcctcgat
    11581 gacatcatca acggtcttca aatgggggat aatgttgtcc tgcaggttga cgacatcgag
    11641 gattacaaaa aatttgtcga gccgttcgtc aaaacctctc ttgcttccag gcatcggctg
    11701 gtttacatgc gctttgccgc tcacccgcct ctgctggagg caaacaaaaa aatcaaggta
    11761 tacaagctca acgccaacaa cgggtttgaa tcgttttcga cacaggtgca caacattatc
    11821 aaggaagaag gacgggatgt gttttatgtc ttcgactgcc tgtccgatct gttgatggcc
    11881 tgggccaccg atctgatgat cggaaacttt tttgtgatca cctgtcctta tctgttcgaa
    11941 ctaaacacag tcgcgtattt tgccattctg cgcagcagcc actcttttaa aaccgtagcg
    12001 cggattcgcg aaacaacgca ggtgctgctg gatattcaca acagcaacgg ccagttgtgc
    12061 gtccacccgc ttaaggcatg gaaacgctac acgccgacca tgttcctgcc gcacatcatg
    12121 gaaaatgata aatttgtccc gatattaaac agcgcggatg cggcaagttt tctttcctat
    12181 ctcacgaaca aaaacaccac gggcggcgtg cgcaatctgg attattggga ccgcctgttc
    12241 ttaaaggcca gtaatcttct tgaagacccc gacgccgtcc ttgatagaca ggaaatggtc
    12301 gattctcttt cgagagtaat gatcgggcgt gaaaaaagaa tgctttcgct ggtcaaggaa
    12361 tacttttcac tggaagattt gctggaaatc aagaaacgcc tcatcggcac ggggtttatc
    12421 ggcggcaaat cggtcggtat gctgatggcg cgcaatattc tccgcaagga ccccgccttc
    12481 gactgggatt ccgaactgga gttgcacgat tccttttata tcggctcgga cattttttat
    12541 tcatatatgg tgcaaaacgg ctggtggaaa ctgctgatgg cgcaaaaaac cgacgaggga
    12601 tattttgaag tcgcccggga gcttaaaagc aggatgcttc acggcatttt ccccgacgaa
    12661 atcaaagagc agtttcagct catgctggaa tatttcgggc agtcgccgat tatcgtccgg
    12721 tccagttcgc ttctggaaga tgccttcggc aacgcctttg ccggaaaata cgaaagctat
    12781 ttctgcccga atcagggcac gcctgaaaca cgatataaaa actttgagga tgccgtaagg
    12841 aaaattttcg catccacgat gaatgaggac gctctgacct atcgtgttca acgcggcatg
    12901 gctcatcagg acgaacagat ggcgctgctg gtgcagcgcg tttccggatc acatcgcaac
    12961 agatatttct tccccgacat tgccggtgtg ggcctttcct ataacacgtt cgtctggcaa
    13021 aaaggcatgg atccgaaagc cggaatgctg cggatcgtct tcggcctggg cacaagagcc
    13081 gtcaatcgcg tggaaaacga ctatccccgt attgtggcgc tcgacgcacc gctgatcaaa
    13141 ccccacgcta aacaggagga tatcaggcgg ttttcccaga acgaagtgga tgtcctgaat
    13201 ttgaaagaca atcatttcca gacactgccg acttccgacc tgctggacta tgccatcgaa
    13261 ccccatatcg gtatgattgc cgtacgcgat gcggccgttg acgaatatta tcagtcgctg
    13321 ggacgggaag cgaaagaaac ctggatattg acctttgatg aattgctgga aaatacgcct
    13381 tttgccagga caatgtccga tatgcttaaa aaactggcgc aggtttaccg ctatccggta
    13441 gacatcgaat ttacagccaa cttcaatgca gagggaaaac ccaaaatcaa ccttctgcag
    13501 tgccgcccct tccagaccaa gggacactac agccgcgtgg aactgccgga aaaaatcgaa
    13561 aagtccagaa tcctcttaag gcaggaagcc aatttcatgg gcggaagcgt ttatcaagcc
    13621 atatcccgca tcatttatat tgatcccaga ggctatgcca gactgacggt ttcagaaaaa
    13681 cacgaggtcg cgcgacttac gggcaagctg aacaaacaga tcggcaagcg ggagaaaatg
    13741 cccacaattc tgttcggtcc cggacgatgg ggcaccacca cgcccgccat gggcgttccg
    13801 gtaacctttt cggaaatcaa caatatcatg gccatcggcg aaattgcgta ccaggacggc
    13861 gcgctgattc cggatttatc ttttggaaca cattttttcc aggatatggt agagatggac
    13921 attttttata tggccattta tcccgaaaaa gagggcgttt ttttcaacac gtcgtggatg
    13981 gccaaacagc ccaacattct ggccgacctg atgccggagg acgggcgctt tgcgaaagtg
    14041 gttaaagttt ttgatatgcg caaccaggat ttacggctcc tgtcggatat tgttacacag
    14101 aagatgattt gtttttttgg agatgcatca gcaatcggaa aatagttgac ttatgatatc
    14161 ggaaaatatt cgtcgccttg ttaaaaccgt tgttcatttc gccggtcgct gccatatata
    14221 tgtcggccgc cgtcccgacc atgtcgcaag accttctctg atttttttcc cggttcttga
    14281 cgggtattta tgttgcgggt ttgccgggtt gatgacctgc ggcctgaaaa gtcagccgcc
    14341 gcaaacccgg gccgatctca cgttggtcgc cctgtggaaa aaacttaaat ccgccaacgt
    14401 gaaaaatcca tccaggctgc cggatgtcac cgaaaccgtc aacgccatgg cagataccgt
    14461 cgcgcaactc aaaaaagaag aggcgcagga atttcttttt tttcatcccg attactcctc
    14521 tgatttgtcc gttttcgccg ccgacctgaa tcgttttatc gcagaaaaag aaaatcggct
    14581 ggatgagcag acagcgttcc tcacctcggc ggagctcgaa tccgtcaaca gtctcatcgt
    14641 gatgctcaag gatattggct gggcgctgga gaaggacatt ctggccaatc tgcccaaaat
    14701 tctggcgctg acaggcgccg gaaaccgtgc cgatatccgg ccggccgcat tcagaaaata
    14761 ccggaaactg aacctggcgt taaacgcttt ggaccgcctg gaagtcagag gccgggattc
    14821 cgcggggatt gatttgactt tcgctccgga aagcgaaaac gatctgaaag cagcgcttca
    14881 acggataacg gaacagggat ggcttgccga ctatgaaaaa agaactcagc cgggagatct
    14941 tctcaatctg tctgtctccg tcacaaaaaa cacccgcggg acgaatgccg gcattgtcgt
    15001 cacgttcaca tataaaacgt tctccacggt cgggaaactg ggacggaatg ttgccgagct
    15061 gagaaattcg atacgcaacg accgcatttt aaggtgcttt gccgaccttg agacatcctg
    15121 tgaaaatgtt ctgatgcata cccgctgggc ttccgtcggc tccatcacgg aagaaaactg
    15181 tcatccggtg aataatcatt ccatgatccg ttttgcctgt ccttctaccg ggtatccgga
    15241 aagcgatgtc cagatccatg tcgttctcaa cggcgatgtg gacaactaca cgccgttgcg
    15301 gcagtttctg gaaacccaag gcgagatgat tgcccggccg atcacgacag acacaaaaat
    15361 cattcccctg cagatagaaa aatttctcca ctcggggcat acgctgactg aagccttccg
    15421 ccgtgccgtc aatgatttgg ccggctctca cgccatcgcc atgacgagcc atctggagcc
    15481 gggcaagatg tttctcgcgc tcaggggaag cggccaatcg ctgtatatcg gcgtcagtga
    15541 cgatcaatat atgttttcct ccgaactcta cgggctggtg gaagttatgc cgcgttttct
    15601 caaaatggac ggcgaagcag gcggccagat ttgtattctt gatcaaaacg gtccggcagg
    15661 cgtgcgcggc atcacgtcct attcctacga cggcaaaatt ctggcgttaa cggaaaatat
    15721 gctcggaaca gccgaaatca caacccgcga tattgatcgc ggagattatc cgcacttctt
    15781 cctgaaggag atttccgagt cgtctctgtc cgtcaaaaga accctgcggg ggaaataccg
    15841 catgaccgcg acagaagcgg cgcttcagca ggtccgattc aacctcggcg aagatattgt
    15901 tcccgccgac atcattgccg gattgaaaaa aggcagaatc aagaatatcg tgatcatcgg
    15961 acacggaacg gcggccgttg ccggtcaggc ggtggcggac gctttttccc tttatctggg
    16021 atcatcgccg ctccatatca cagcgcgcgt tgcctcggag ttgagcggct tcggtctgaa
    16081 agatgatctg tccgatacgc tggtcattcc gattacacaa tcgggaacaa caaccgatac
    16141 caatcgcgcc gtggcgatgg ccaaagaacg cggcgcccac gttatttcga ttgtcaaccg
    16201 ccggcaatcg gacatcacgg ccaaatctca tggtgtgttc tacacaagcg atggacggga
    16261 tattgaaatg tccgtcgctt ccagcaaggc tttttacgcc cagatcgtcg ccggacaggt
    16321 gctggcgctc ttttttgccc agagtctgcg gacctgcacg gatgaagata tcatcgcctg
    16381 tttgagaaat ctcgaatcgg caccgctcct gatgaaccaa atcgcgtccg actgcgcgtc
    16441 ggtggcctcg accgtgaagg atgccatcgg caaaaaatac tgggccgtta caggcagcgg
    16501 tccgaacaag gccgccgcgg atgaaatccg catcaaactc agcgaactct gttacaaaac
    16561 catttcaacg gacatcgtcg aaaacaaaaa acatatcgac ctgtccgccg agccgctgat
    16621 tctggtgtgc accgcggggt ctcctgaatc ggtcaccgcc gacatcgtca aggacgtggc
    16681 cattttcaaa gctcacaaag ccaacgtgat tgtcatcgcc gatgaaggcg aaaaacgttt
    16741 tcaaggcgtc gccgatgccg tgatcgccgt tccggttgcg tttccgccgc tccccgttat
    16801 tcttaacgcg atggccgggc atttatgggg ctactacgcg gcctgcgcca ttgatgagga
    16861 agcacaaatc ttccgggcct tccgagcccg tcttcatgat attttaatgc taaaggcgcg
    16921 tgagcaactg tccgtttttg acctgacggc ggatgtgtcg gtcaaaggaa tcatcaacac
    16981 gttctaccag tcgttcaaca cccgtcgcca acagggcgcc ttcagttctc tggagggcaa
    17041 aactctctcc gatctgatgc ttctgctgaa atatgcagcg ggcaaactgc cgctgaagga
    17101 tatggctctg gatttcgtca acgaccaggg catcttttca ccgtttgagt tgctcgatac
    17161 aaccctgggc acggtcattg acgaattgtc gcgtcccatc gacgccatac ggcatcaggc
    17221 aaagaccgtt accgtcggca cgagccgaaa agaaaggccg ccggaaggca ttgtttttga
    17281 tctgctgaaa acgctcacct ttgacatcgc gactctgacg tatcgggata tcatgatgat
    17341 caggcgcatc cagcccgtca ttgcatcgat tccgggatac acgatttatg acatcagcgc
    17401 tctggaccag cagggcaatc cgacagacga ctcgctgatc gccgtacgcg tgaaaggagg
    17461 ccttgcggcc ggaatgaaat cgcgcgccga caagcctgcc gtgctgatgg gggtgaaaag
    17521 aacaatcgtc agcacggggc atgcctatct gggcagggga aaaacggaca acgcccccat
    17581 tctgatcatc ccgttgagaa cgcaggaagc cggaattggc aatctcctgc tgctgcatgt
    17641 tcgattcgat gaaaaactga ctcttcagga aaaaattgac gcgctgggat acagatacaa
    17701 cgacattcgc aatcttatcg acgaatacaa tctcaaatgg gatgaccggt ttattgaccg
    17761 gttttcattg gaggcgctat ttagcgaacc gatcgaaatc atcgccggca ggattaaatc
    17821 ccaactgaat ccgtagtacc gtattgacgg taaatcccgg attaaccgat aatgagttct
    17881 aaacattttt atcgtttttt tctttttgga tcagacgtgc ctttatttgc cgggatattc
    17941 tctgttttga tgttgggaga cagtaaatca ttgatattca gattaacgga gaattgcctt
    18001 gattccttct taatgctgtt caggatgatg ttcacaatcg catcggtgta ttgaggcaga
    18061 tttttgggtt caccgagcag cagcttccgg atgcacagat gttcaatggt gcccaaaagc
    18121 atggaccgga caaggaaagc gtcggtatct ttcttgaatg ttccatcttt aattccttcc
    18181 tcaataacct ccagaagccc tctagccact tttctgacca tcccgtatgc cttggccttt
    18241 aaaaattttc tattggtctt cagctccagc atcaccaggg aagcgtagag aggattctca
    18301 tcataaacgg tcagatatcc ttgcacaatg cccctgattt ttgcctccgc tccgcgaaga
    18361 tagggagcca cgtattccat gatgcgtatc ggctcttcca taaacttttc aggaatggca
    18421 aaaagcaaat cttctttggt cttgaaatat tcatacaaca cgggcgtgga cacgcctgcc
    18481 ttcctggcaa tgtcggtcat ggttgtgtct gaataacctt tctcggcaaa tacccgcagg
    18541 gcggcatcaa taagattatc tttccttgct tccttgatat cgtgtctggc catagcctct
    18601 ccttatccgt tcagtgccgc ttatctaaaa cagatcatgt caaaacacaa gcacattcat
    18661 ggacttttat gccgggatcg cccaaaacac ctctcaacca aatatcaatt attatttatg
    18721 ttaataatat taattcacct aataactaat gtcaataaat atttcttgac atttaattat
    18781 catgaattaa tatttataca tatattttaa acgttgataa ttatataccg caaatacaag
    18841 acatgaccga aaggagcgtc tgatgaggac aaaagaacaa tacatcaaag gcctgcggaa
    18901 aatgaaaaga aatctttatt tcaacggcag taaaatcgat cgggatgatg aggaacagct
    18961 tcccgccttg aatgtcatgg gattcacgtt cgatgctccg catattcctg aattcaagga
    19021 tctgtgcacc gccaagtctc atcttaccgg cgaaaccatc aaccggtttt gtcacgttca
    19081 tcaaaactcc gatgacctgc atagaaaaca ggatatgaca cgtgcgctct gccgcaccgg
    19141 cagaatgtgc attcagcgct gcatgggcgt agatgccatc aacggggtca atgcggcaag
    19201 ttatgaagcg gacaaacaga acaatggcag taccgagtat cataaaaact ttctcaaatg
    19261 gctggaaaac tttcaaagaa atgatctggt gggctgctgc gctcaaaccg acatgaaagg
    19321 ggaacgtctt aaacgtcccg ccgagcagac cgatcccgac atgtatctgc gtgttgtggg
    19381 aaagaagaaa gacggaatcg tcgttcgggg atgcaagcta cacatttcgg aggcatccat
    19441 tgctgatgaa attatcgtcg tgccgacgag aattctcggt cctgacgaac agcaattcgc
    19501 ggtgtcgttt gcggtgccgg cagattacga tggcgtcaaa caggttgttc atattcacaa
    19561 aattcgcaag cggacccatt tcaaaaccgg aaaccccgat atcggttata ccgattccta
    19621 cgtgattttc gatgatgtgt ttgtaccgtg ggagcgggtt ttcctctgtg gcgaatatgt
    19681 acacggcggt gctttagcca tgctttttgc gcttttccat cgtcacagtt actcaggctg
    19741 caaaccggca atcggcgaca tgctgctggg cacagcttcg ctggcggcaa aaatgaatgg
    19801 gatatcgaaa gcgcatcatg tgcgggaaat cctttccagg atcattatgg ttaccgagct
    19861 gggttacgcg gccggattta cggcgtccgc caagagcaaa ccggaaattt atgttcccgg
    19921 catcggccag atgccctatg gtcccggtag ttatattccg cattccatct atgccaacgt
    19981 aggtcgatgt ctcaccggag aagctgtttt tgatgaacag gaaatgctct gcaacatagc
    20041 tggcggcctg ccttcaacat tcccttatga agcggatctc gccaatccgg aattgaaacc
    20101 gcttctggaa aaatatctca aacgcaaccc caaaatcccg atagccgatc agatcaaatt
    20161 ctggctgctg ttcgcggaag caacctgttc atccagtacg ggatttatgt gttacgggtc
    20221 ttatcacggc ggcggctctc cgattatgga gcagattgcc atcaccttgc agtacgatac
    20281 gaaactgcgc gaacacctgg tgaatgctgc ggccggcata gagaaactcg attacggccg
    20341 tgtgacaaaa atttagataa attttttgcc cgggcagccg gtcgggcaac cctaaacacc
    20401 ttttgattca gcggcacaca agaaagggaa acaaattctt ttattaaagg aggtttgtga
    20461 tgaactatcg tgcggacacg ctttgcggac tttttcatca tcaggctctg cgttacgggg
    20521 atcgattcgt ttttcttacg ggccgttttg atgccgacgg acgcgcctgt gatcaattca
    20581 gttcgcgtac atggaaacaa gtacgcgaag aagccatcgc tttggcgaaa gggttgatcg
    20641 cactgggact tcaaaaaggg aagaaagttg tcatttattc cgaaagccgt ccccgctgga
    20701 tcatcgccga tcaggctgtt caggcctgcc gcgccatcgg cgttcctctt tatccgaccg
    20761 tttcggagga agaactggat tacatgctcg aggacagcga gtcctgtctg gcgattgtct
    20821 ccactccgga aaaagcgaga cgggtgctta aagtaacggc aggcaaagcc aaaatgcccg
    20881 tgattatgat gagcccctgg gatgatatcg gtcaaaaacc tgaaggcgtt tacaccttcc
    20941 cggaggtatt tgcactgggt gagcccaagg taaaaccgga cgtgatagaa gaggggataa
    21001 gaagcgtcgt tcccgaagat gtcgcgtcga ttatttacac gtccggaacg accggtaagc
    21061 agaaaggcgt gatcctaacg caatccaact ggattcattc catgcaccag tgttccggct
    21121 ccgaaatcat ggcgctgacg gcccaaaaag atttgcattt gaaggcgctg gttcatctgc
    21181 cgctttgcca tgtctacgga cgaatgagcg attatcatac cgcgggactg aaaatgggcg
    21241 gtgaacttgt ttttgctgag agctatcaga ccattgcccg tgacctgcgg gaggttcgtc
    21301 cgaatataat caacagcatt ccccgccttt atgaaaagac gtatgaaatc gttcagtcca
    21361 ccctttccag ggccaagaaa ccctatcagg cgatctataa ctgggccatg agcaagggca
    21421 gaatttatgt agactgcatg gccacgggaa aacgcatgcc gcctcatcag cttatgcttt
    21481 tcggcttggc caacagcctg gttttcgacc gtctcaaaaa agaaatgtgc atggacaaac
    21541 tggtgatggc ctgttccggc ggcggaaaac tgtccaaaga tatctgtatt ttttacagag
    21601 ctctcggcat ccaactgatt gaaggttacg gcctgacgga aaccaccgcc atcaataatc
    21661 tcaacgcgcc ggaaatcatg atggagaaac cacccaccgg ttttctcaag gttctctatg
    21721 acaggattat ggccctgaca ttgcatctga tggtggtgcg gcagtcgcag ggcaaatcgc
    21781 cgtatgccaa tccggtcatg tccctgctgc tttcgctctg ctacaatacg ctcgtttacc
    21841 ggttgcgggt gaagccgggc ttcgtcggac gtgttgttcc cgagacggaa atgcgcatcg
    21901 ccgaagacgg cgaaattctc atcagggggc cgcaggtgtt ccagtgctac tggaaacggg
    21961 aaaaagatac cgcggatgcc tttacgcccg atggattttt catgaccggc gatatcggtg
    22021 tggcggacga ggagggcttt ctgcaaatca ccgaccgcaa gaaggaactg tttgtcacct
    22081 ccggcggcaa gaatgtggct ccgcacccga ttgaagtggc cctgattgaa agaccgtaca
    22141 ttgatcaggc ctgtctgatc ggcgacggac gcaagtatct gacggctttc atcatcccgg
    22201 attttgaaag cctgaaacgc tatgccaagg atcatggcat cacctgcgct tcacaggagg
    22261 acctggccaa tcatccggat atcaaggcgc ttatcacgaa ggaagtcgat catgtcaatg
    22321 ccactctggc gcgttacgag cagataaaat attttacgat tctcggccgc gcctttgatg
    22381 ttgcctccgg cgaactgacg ccgaccatga aaatcaagcg ccgggtcgtc aatgaaaaat
    22441 atcagcaaca gattgaggag atgtacggca aaccgaatca ataaataaaa tcagtttatt
    22501 gtttattatg gagaaaaagc atgatcattg actgtcatta tcatctggaa gaaagggttt
    22561 ttacagttga gcgtctgatc ggcgaaatgc agaaagccgg catcgacaag gttgcgctca
    22621 tgggcagcat tatcggacca tttacggagc ctcctcgcat tcttatcaat cttttacaac
    22681 gcttactgga aaattctttc tcgcgaagta tagggaaagc atttgtttcc aattttaccg
    22741 accatggcga tgtcaggatt ttgggaaagc catatcccct tgcgacggat ccggataatg
    22801 aaaaggtatt taatgccgtc agaaagtatc cggataaatt tctgggctgg atttttgtaa
    22861 atccccgggg caaaaataat caggtcgagg agtatgaaaa atataaagat gaaaaaggat
    22921 ttatcggcgt caaggcgcat ccgttctggc atcacttcgc acccgtggaa ctcgctccgg
    22981 ttgccgagag gctggccaaa accggcaagc ctcttctgat tcacgcggga ttcggcgaag
    23041 aaggaaatta tgaagctctg ctcaaaaaag tgccggacct taagttggtt ctggcccacg
    23101 ccggtttccc ctgttactcg gatacgtgga aaaaaatcaa gcccctgaaa aatgtatata
    23161 tggatctttc tcaaaccact tataccagcg ggaaagccac ccgccaggcg gtggatatgt
    23221 tcggtgcgga aagacttttt ttcggcacgg acggtccgtt tggctttcac gggcccgatc
    23281 atcaatacga ttacggcttt atcaagcgca gaatcgaggg catgtttgca gatgtcataa
    23341 tccgggaaaa gcttttgggc gggagcttcg caaaagtcgt cggtctgagt taagaaatgt
    23401 tgacatgatt tgaaatgaat gacaaaaaaa catctggtgg atcaaccgcg ccatccttcg
    23461 gtgtccaggt ctctggatga tccgattccg actgccgcct ttctgcttac aagacatcca
    23521 ggccgcctca cacgccgtta cttgatagga atccaaaact tttgactctt ccgcatgtca
    23581 ttcacaccgt tttgcctatc cggtcctgaa tacatcctca cgaatgacgt gtgatatccg
    23641 cttttggcgc agaaccgccg ccgcagatag atgaatgccg gagacgaaag aaaatgatca
    23701 tcgtgtaaag gtcttgtgca tattatgaaa cggtccggcg ggaaatcgat cagacttttc
    23761 gtttgcatcg gagaataatt cgggataagt tgcaaaaaat ctctacggga aaacatgact
    23821 ttctcccatc aggtgatttg atggtcttgc ataaaattaa acaaattgtc ggctggttgc
    23881 gaagtttttt tctggaaaat atcacgcgct ggctggtgtt cggtctgctg gtcggtattg
    23941 tgtcgggtct gggagcttcg gctttttatt atcttctgca ggcaggccgt catctttgtt
    24001 ttcatctgct ggcgggttat gccgttcccg caccagcggg tgaacaggtt ttcgacgtcc
    24061 ccggcatact acaatttcat cccctgattt tcttccttct gcccgctctg ggtggtttga
    24121 tcagcggctt actcgtgtat cattttgcac cggaagcaga aggacacggc acggacgcca
    24181 tgattgatgc gtttcacaat aaaaaagggc gcatccggac gcgcgtgccc gttatcaagg
    24241 gattggcctc tattatcacg ctttccagcg gcggcagtgc aggaagggaa ggaccgattg
    24301 cccagatcgg cgccggcatc ggttcggccg ttgcgcgtct tttaaaattg agcaaccgcg
    24361 agcgccgcat ccttcttctg gcgggctgcg gaggaggtct atcggcgatt ttccgtgctc
    24421 ctttaggcgg cgcactcacg gccattgaaa tcctttacaa ggaagacatg gaaacggaag
    24481 cccttgtgcc cacggtggta agttccatta ccgcctacat catttttacg tatttttttg
    24541 gcaacacgtc gattttcttt tttccggagt atacgttttc caatccgcgc gagctgatct
    24601 tttatgtgct tctgggtctg atctgtgtcc ccgccgggat ttttttcatc aagatatttt
    24661 acgcggtgcg ggagcgtatt ttcctgcccc tgaagattcc ccgctatgtc aagccggctc
    24721 tgggcggtct ggttgtgggc tgcatcggcc ttgtctttcc tcaggtttac ggcgacggct
    24781 ggggctggat ccaactggcc atcctgggga aactcagcat cggaatcatg atagcgatcg
    24841 ccttggccaa aattgtcgcc accagtttta caatatcgtc cggtgggtcc ggcggtgttt
    24901 tcggcccgac actgtttatc ggaggaatga tcggcggcac tgtaggattt tccgcccatt
    24961 atttttatcc gcatattgtg acgcagccgg aagcctttgt ggttgtgggc atggcgtcat
    25021 tcttcgcggg ggtggccagt gcgcccatcg gaacactgtt aatgtgttcg gaaatgaccc
    25081 gaggatacgg cctcattgca ccgttgatgc tcgtgtccat cattgccatt ctttttacac
    25141 ggcagcactc catctataca aagcaggttc aaagcaagcg tgaatcgccc gcgcatatgg
    25201 cggaattcac cgtgaacctt tttgccgaaa cccaggtaaa ggacttctac aagccggaga
    25261 aggtcacccc tgtccgtgaa atcacgactt acggacagct gaagagaatt ttttctgcat
    25321 ccaatgtcga atgttttccc gtgtacaatg aagatgacgt gctgatcggg gcgatcaatt
    25381 gggaccacgc ccggtccatc gtctttgaag aaggcctgga agatttagtc attgcgcagg
    25441 acctgatggc accaccgcaa accgtaacgc cggaagacaa tttttatgac gcgtttgtaa
    25501 aattcatgga aaccaatgta gaggaattgc tggtggtgag ttccgacgat cccaaccggg
    25561 tgctgggagt tttaaggcac gacgatctga ttgccgcata tcacgcggag ctgaacagac
    25621 gaaaatatga ataggcatga atggattgat aaaaaaatct tttttgtcgt cctgcggaca
    25681 tgttcttcta aaaaatagta gattaattct aaatatttct acgggcaatg attgtttcgc
    25741 attccgtcgc taagtcatta agcattttaa gcccccccgc gttgtccact gccgtcaata
    25801 aattctccag cccgttggga atgtggtgaa gaaatgcggg cttgtgtttt ttgagtccca
    25861 gatatccgta cgcccccagc gcctgtaaaa gccgctgtac ggaacccgcc cagaaattat
    25921 gagcgaactc atcgcggcta taggcgggac gaataatgtc ataataaaaa tccagcaact
    25981 catctcgttc tttctcttca aaggtgacgt atggatcgca gagaagagat ccgagatcgt
    26041 aaaaaagaca gccctgacgc atgccctgaa aatcaatcaa tacaggcctt tcatttttaa
    26101 tcatgatgtt ttgcgactgg aaatcgcggt gaatcagcga cggttctatt ttttgcaggc
    26161 tctcgaaaag cgcgtccagc tctttattca gtttcgcggc atccgcagat gttagtttta
    26221 ttttgcagac ttcccatgcg agattttcca ggaaatagtt atgctcccat ttgtaaagcc
    26281 ttggaccgta actttcggat aattgcacat cgggcgaagt ggatttcaga tcaaaagaat
    26341 gcagacgatg aatttgagcc aacacctgaa aatagtgatt gcgtctttct tcccagggtt
    26401 caaatctgag cgaccacaaa tcaacatcac ccagatcctc aatcaaaata aaatgccgag
    26461 ggatatcccg gagaataatc cgcggcacat tgatatccat agatgccatg aattgattga
    26521 tgccggccca gttggcattt tccacaacct catcgccgta gcgcatgaaa ataaaactgt
    26581 ccccgtgagg caatgacacc ctgaagaatt cacggtctga accgcccttc ttgatcgact
    26641 ccagcttaaa atcggttttt cccagatgtt cagttaaaaa tttttgaatt tccttctgca
    26701 tgagcttcac ttaataatgt tgtcatgacc cggtctgagc ggctaatcca aaactgaaaa
    26761 atactggata ccggccttcg ccggcatgac aattttgaat gagatttaac gaatatctgt
    26821 tttgctcaat ttttcatatt cagcgacagt accaacatca taccaaccac catcgtcaat
    26881 gataacaccg ccgacagagc gtggattttc ctttatcatc tccaccagcg gtaaaacaat
    26941 cgattcgatt tttcccgtcg ttaaccgttt caggaaagat ttttccacaa tataaatacc
    27001 ggcaaacaaa catttcttga cgccatcatt tttcaaaata tgccgcatgt cgcagacaaa
    27061 gccatcttta tcaacactga cattgagcag cggacctgtt gagcgcagcg ccagggtaac
    27121 ggacgttttt aactcaaaat gttttctaat cagcagttcg atcggcatgt tggcaatgat
    27181 gtcgccgttg taaacgataa ttcgttcatc ttcggcaatc aggtcttcga tatttttgat
    27241 gccgcctgcc gtgtcgagca gaaccggttc atggcaaaac gtaatcggag tgccctgcca
    27301 attgccttcc ggaaaagcct caacgtattt ttccgcgcag tgatgcgtgt tgataataaa
    27361 ccgtttgata ccgacagcgc gcagatgcgc cattgcataa gtaatcatgg gatgtccgcc
    27421 gatttccaga agcggtttgg gtgtattttc cgtcagcggc ctgagcctcg ttcccagccc
    27481 cgcccctaga ataaatgctg tgttgagttg tttgttttgc atatagtttc acccgcgtgg
    27541 ttctttgttg attttcttcc catactcagg atcaatcttc accagcgctg caacaataaa
    27601 acccggaatg gttgtaatca gaatccagat gaagaaattt ttgtagccga gcatatcctg
    27661 aatccagccg ctgcccatcg ccggaatcat catgcccagc gccatgatgc ctgtaccgat
    27721 ggcgtaaaac acggttttgt aactgccctg tgcaaccatg atcatgtaca tggtgtaggc
    27781 cgtaaaaccg aatccgtaac cgaatttttc gaccgccacg gcggaaccga ccagccataa
    27841 gttctgcggc tggtaatggg acaaatacac aaaaatcaaa tccggcaggt gcatgatgaa
    27901 aaccataggc cacagccaat attttaaacc gtttctgtaa atcacataac cgcccaaaag
    27961 accgcccagc atcagcgcaa tgacgccgac cgtaccgtaa atgatgccga tatccgctgt
    28021 gctcaagccc aatccccctt tgctgatttc atcgcgcaga aaaggcacga ccattttcgt
    28081 cagttgcgcc tcggcaaaac ggtaaagcaa aaaaaaggtg atgatgatca gaatatcctt
    28141 gcgccggaag aataaggtca aaacgcggaa atattccgca aggaaatttt ttgatttgtc
    28201 ccacggcgcg ctgatgtctt cggcgggctt cggcaatgca aatatgtgat aaataaaaaa
    28261 cgcgagaaaa accgccgcca cgacgataaa ggatagcgac caagcgccga ctgccggata
    28321 accgtatttt tgcagcatac cggccagaaa caccagaccg ccttcagcgg caatagtggc
    28381 aatacggtaa aaaataacgc gcacaccgac aaatgcagcc tgctgcggcg tgtcgagtcc
    28441 cagcatgtaa aaaccgtcgg cggcgatatc gtgtgtggcg gagctgaagg ccataatcca
    28501 gaaaacagcc agagagtagc gcacaaaatc ggttgtcggc agcgtcaggg cgacaccggc
    28561 aaacgacgcg ccaatagcca attgcatcgc cagtatccag aagcgcttgg tacggaaaat
    28621 atcgacaaac ggactccaga gcggtttaat cacccagggc agataaagcc aactggtgta
    28681 aaaagcgatc tgcgtgttgg aaagccccat atctttgtat aggacaacgc tgactgtcat
    28741 agccatcgtg tagggaatgc tttcagctaa atagagagaa ggcacccaaa gccagggatt
    28801 cctccggacc gcatttcttg ttgtttctgt tattgtttcg gacatttcct ttctcttttt
    28861 tatgtcactt caactgaaag ctacagtcca gctatgccgc gatgaagtct taatattatt
    28921 ctgcaacacc atctgaagga cggctcactc gcactgataa ttcaattacg gcttatcaat
    28981 acagtttttt taactgcgcg ccggtgaagt agtgcgtcag aatttcttcc gcttgaaatc
    29041 ccttggaggc catcgccgcc gcaccgatct ggcacagccc cacgccgtgc ccccagcccc
    29101 cgccgatgaa aacaaaaccg ataacttctc catacgtatt acgccgaaca gtcacaacaa
    29161 aagcgctgct taatagatga ctcggtgaaa gccagcggcg aatttccagt tcctttccga
    29221 ccatcaccgt ccttttcgag ccgtcgattt tcaggcgtga gatcctgccg gacggcccgc
    29281 gttccagcgg ctcgatattt ttcagatctc cgaaatcaat ccctgacttt tccctgatga
    29341 tttcttccag gtcttcccgc tcataggcga cctgccagcg gaaaaaatcg ggcgtttccc
    29401 gatcaaaagc cggcaggatt tgtttcaata atttttcatc ggttgtattg caataagcct
    29461 tcggcctgcc ggtcaaccat cgtctggcgt cgccttccgt gcacacggga gaatgcggtt
    29521 tcacatcatc gctgatattg ctcaaataat cataatgctt gtcttcccac gccgtatcga
    29581 aaacatctgt ctgaccgcca cagcatttgt aataacgcgc gtcgcaaatt tttcctccat
    29641 aaacgagaaa atgcccgcgg gtactttcta tcgccttatg gacattttcc gaaattattt
    29701 ttgtgattcc ctgatagcgc tggcaatggt cgtcggcgca cacatcaaaa ccatcatgat
    29761 cgttgacatc ctgccagaca acgatttcat ttccggttct ctgaggtgtt gatgtccggg
    29821 cttcctgctt tttggcaagc atcgacacca gccagcttcg cgcagtaatt gcctgtgctc
    29881 tcaaaaactc cagaggcgct tcggcggaca tctccgaaga gatgacactc tccagataat
    29941 tttccagaag aacattgttg ataagattga aagaagcatc tcgtgaagcg gataaaaaca
    30001 aatcgccgcg aaacgattgc tcctgagtcc gctgccagtg gaaatcaatg ccgattttta
    30061 catccgatac ggtaaaaaaa gaacggcttc ccgcttgcaa aaggatttct ttctgccgca
    30121 caatttcttt tcctgacgaa tcggcaagca ggacccaacc atcatcaacg gtaacggtat
    30181 agcaaccttc caatatccgg ccatgcgcag atgaaaaatc ccctttcaag gtgatatgag
    30241 cttcgctgta attttgcaga agggcaactc tgatttcagg ttcgtaatgg atcatggttt
    30301 gttttttatg aataaaagaa agagaagtca agaaaggaat gtattcccca tcgtgctctc
    30361 cgggacattc ccgtgccctc cgcaacatca ggccattcaa cgaacgagaa actttccatg
    30421 acgccgcgtg cggaatcatt caacgcacag tgatcgcctg acgactcccg gactatgcgg
    30481 acgttatggg caacgacttt tttaatttcc cggaaaattg tcagatgaag gaactaattg
    30541 cttgaatatc attgttttat cgtgtatggc tttcataaat tactcattgg atgatttatg
    30601 gcgcatattt ggccaatcgg gggaggcaaa ggaggatcag gcaagagctt tgtggcaagt
    30661 tcgatggggc gtcttctggc caaatccgga aaaaaaacgc tgctgattga tcttgatctg
    30721 ggagcagcaa atctgcacac gatggtggac gttccctacc cggaaaagtg tttctctgat
    30781 tttgtctcca aaaaaatcca tgatctggaa gataccgttc ttcaaacacc ctttcctaat
    30841 ctttttctga tcagcggcgc ccacgacagc ctggacatcg ccaaccttcc ctatgaaaaa
    30901 aaaataaaaa ctctcaaggc cattgtcaag ttggattatg actacattat tctggatttg
    30961 ggggcaggta ccgcttttaa cacgctggat tttttcctgg cctcaagaaa cggcatcttt
    31021 atcaccacgc cggaacccac gtccattgaa aacgtgtacc gtctgatgcg cgccatctat
    31081 ctgcgtcgca tccgccatta tttttcggct gccgacttca ggacgctcga aaaaaaggtc
    31141 cgggaacacc tgggcgagga gtcattcaac aagccggaaa atatcgtttc gatcatcaag
    31201 aaagaatttc cggaaaaata cgcgttgatc agaaaggatt tcaattcttt ccgtttcaaa
    31261 cttattctca atcaactgcg caaacaggac aacaccgttc tgggcgagca gatttgcaaa
    31321 attatcaaga agcatctggg catcaatgtg gaatttgccg gcaacatcgc ttacgatgaa
    31381 cacgttcacg acgctatctg ccagcgcgtg tgctttctgg accggtatcc gcacacgcgt
    31441 gccgccaatg atttgcgtgt tttaaacagg aaaatgagca atgccttcgg tgaacaattg
    31501 atgctcaact atctctgaaa aaattgaatc aggcggcaca tggaaaaaga ttttgaccag
    31561 atgaattatt acgaaatcct ggacatcggt acgaacgcga catctgcgga aatccgcggc
    31621 gcctacaacg tcgcgttgca aatgtatcag cccgattccc tggtcagcta ttcttttttc
    31681 tcgaaaggag aacgcgataa aattctctcg ctgatcgaca aggcttattt tactctgatc
    31741 aacgaagccc agagagccaa atacgacaac gaaatgaatc tgcgggaaac gtccgtttcc
    31801 gcagaccaac acaaagcccc cccccaaccg gccaacatct tcgaatttaa ccgccgggat
    31861 atcgcttccc tgacgcggaa aaacaacaaa tcggcattaa agacaaaaat ttcccaaagc
    31921 cagtccatca gggaaattct ttcccggcag aaaatcaccg gcgccgacct gaaggccatc
    31981 agggaagaac tcgggctggc aattgaaacc atccatcagg aaacaaaaat ccgtcttgat
    32041 taccttcatg acattgaaga agacaaaacg gaaaaactgc cggcacccgt ctttttaaaa
    32101 ggatttgtga aagcctattt gcgaagcctg tgcattgaga atgcagatga tatcagcacg
    32161 gcctacatga acacgttacc cggtaaaaat taacttgaca gcgctaatct ttagtggtca
    32221 ttatttgtta aaatcatagc atgaaaaatt cctgaagcgt cggcaatgaa agtcctcatt
    32281 catctgaaaa tttaccgtcc ccaaaggtgc tttccggttt tttatgacga ttcaaaagga
    32341 gcataaatga aaaaatcact attctattta ctgttaatca gtatgctgat cttttttgtt
    32401 tccgcctgct cggacagcga ttcagatttt gacgatacag ccgacgcgga atatctgacg
    32461 gttcaaatga gagctgatgc cggcaacgga gaagtctcgc ttgactggca gatgcttccc
    32521 agagctgaaa catacaacat ttattatatg gaagatgacg aagacgatac caatcaaccc
    32581 acctcgcaaa aaatgaaatc tgaaggcaca aaaatagatg gagaaacaaa ccacatcatc
    32641 agcgcgccgt atgtagttac cggtctggaa aacgggaaaa cgtactggtt tgccctgtcc
    32701 gccaaaaatg ccgagcaggc ggaaagcgac cttagccagg ctatttatgc gattccccgg
    32761 gctaatccgc cattgcctgc tcctaaaaac gtccgcgcca acgcaggtga taaagaggcc
    32821 actgtgacgt gggatgcggt aaccggcgcg gccggttaca ttgtgtatta ctacacgtct
    32881 tttgcaaact atcaggaaag ccctaaaatc acgagcacct cgtatacatt cacaggactt
    32941 accaacaaac aaacctatct ttttttcgtt gccgcagtcg acagtaacga caacaacgaa
    33001 agcggcgaca gcagcgcttc gtttgtctac acgacaacgc caagcgaaga tccgcctcca
    33061 tccgcgcccc gtaatcttcg ggtcatcaat cgcggtgaag gattcgttga tctggcttgg
    33121 gatgctccat tattccacac aggagcatta acctataatg tctatttttt caccgccggc
    33181 aacattacaa aatccacagc ccaaaaaggg atagaggccc tggcggatac gtctacccgg
    33241 gccgcgaatc tcgataaaga aaaaacgcat tattttgtcg tcacagccgt agacaacaat
    33301 ggagaaagcg cggaatcagg agaagtttcc gcaatccctg atttataaaa aggaattatt
    33361 aagcaaatca attccggata aaaagggctt tttaggccct tttttattat acgtgacaga
    33421 aagtcgggac aatgcgaaac aaaagacacg ggattgaatc gtaataaaat ttatgttaaa
    33481 cgcgcccata ggcattatgg caggcggagc aaaaattaat gagtgttttt gaatacatcg
    33541 cgctggatga aaaagggaga caactgaagg gatttatcga tgcttcaggt atctccgcag
    33601 cgcgtcaaaa gttacgggaa gaaaatattt tccccgtgga aatcaatcag acggaaacaa
    33661 aaaaagaatc cgctctggcc ggggctctga agataaatct ctgggaaagg gtttcggcaa
    33721 accaggtggc tgtctttacc cgccagcttt ccacattact gggagcaggc atgcctctgg
    33781 tcccgtcgct ttccattctg atgcagcagg aaaaaaatcc gcttctgaaa aagtccctgg
    33841 cgcaaatccg tgaacaggtc aatgaaggca aaagcctgac ggaagccatg gcggaatttc
    33901 cccgcatctt tccttctttt tatctgaaca tggtgagagc cggcgaggct tcgggaacca
    33961 tcaacctggt tttagagcgg ctggccgact tcagtgaaaa tcagcaggcc ctgatgaata
    34021 aaatccgttc cgccctagcc taccccatca tcatgtttat catgggcagc gcggttatct
    34081 ttcttttgat gacgtttgtt gtccctaaga taaccggaat ttttacggat atgaatcaga
    34141 cattgccgtt ggtcaccatc gttctgatta aagtcagcaa ttttctgaag gcgttctggt
    34201 ggatcatcct gatccttttg ttcggtggac ttgccgctat tagatatatg acaacgggca
    34261 ccgattccgg cagacgcgcc tgggataaca tccggctgaa aattccggtc atgggacaaa
    34321 tcaaccgcaa aattgctatt gcccggtttt gccgaacgct ggccacactt ttgcaaagcg
    34381 gcgtgccgct tctgacagcg atggaaatcg tccggaacat tgtcaacaat gttatcatcg
    34441 gcgatgccat tcatcaggcg gccagagatc ttgaagaagg caaagggctg tccggtccat
    34501 tgaccaaaag cggtttgttt ccaccgctgg tgacggaaat gattgctgtc ggagaacaaa
    34561 gcggtacact ggagaccatg cttaatcgta ttgccgcggc ttatgaaaac gaatcacagg
    34621 ccagtatcac ggtcatgacg tcgctgctgg aacccatcat gattctgttt atggggctgg
    34681 tggtgggttt tatcgttgtc tcaattttat taccgatttt cgaaatgaat cagctggtca
    34741 gataatttag acatccgtaa aaaagggggt tttatgaaaa ttttcagaag aaaaaaaagc
    34801 agacaggcgg ggttcaccct gattgaactg atggttgtca tcgtgatcct gggtgttctt
    34861 gccggtatga ttattccccg catcatggga cgtcctgacg aagccagaca ggccaaggca
    34921 agaatccaga tggaagccat ggaatcggcg ctgaaactct acaagctgga caacggcagt
    34981 tatccttcga cggaacaggg gctaaaggct ctggtggagg ccccgacaac gggagatctc
    35041 ccgaagaact ggcgtcaggg aggatatctg gaaaaaggga aagtgcccaa agacccatgg
    35101 ggaaacgatt ttgtttattt aagtcccggc agtcacggcg attttgattt atcctgtctg
    35161 ggcaaggacg gtgaagccgg aggagaaggt gtcgatcagg acatcaacaa ctgggaagtg
    35221 gagtaagcag ggattcacgc ttgttgaatt actgcttgtt atcacgctga tcggcatagt
    35281 tctggtgctg gccgttccgt ccacacggga cgttctcacc ggcgacatgc ttaagaaaac
    35341 gtcgcgccag ttgatcggcc tggaaagaaa actcagaacg gaagccgttc gcgatcagct
    35401 ggactatatt ctccatctgg atttatccga ctccgtctac tggattacgg cttccgacat
    35461 gacagagatc aaacaggctg aaatcaaaaa acaggctctg catttgcctg aggatgtccg
    35521 cattatcgat gtcgtggacg aagacaaccg gaagattacc gccggcgaag taaaaatcgt
    35581 cttcaaaaag gataacatct gttccccgag agtgattcat ctggcttaca aggaagataa
    35641 aatgacgatc gtcatgaatc cctttatggg tgtcaccgat atttacgacc agtacgtgga
    35701 aattcctttc aacacctccg aagggcaaaa tcgccggtga ggaccaggct atgacgcgaa
    35761 tgacagaaaa aggatttact ctgatggaag tcatggtcgc catggccatt ctggcgattg
    35821 cgctggtttc catctttcag cttcagtcgc aaagcatttc catggcgacg gattcgcgtt
    35881 tcatgacgac cgccgcactt ctggcccaga gcaaaatggt cgaggttgaa acacagagcc
    35941 ccttatccaa taaaaccgaa aacggcgatt tcggccccga ctatccgcaa tatgcctggc
    36001 agttaatcat tggcgatacc cagttgcctc aattcaaaaa aatcgaggtc accgtcacca
    36061 acaaactatt tcttcgccgc ggaacgtaca cactgatcct ctataaaagg ccggatatgt
    36121 aactatgaga cattgctctt ccagacccca tttaagcggc ggcggattca cactcgtgga
    36181 aattctcatc gctatcttca ttctctctct ggtgctggcc accgtttatg tttcctatac
    36241 cggcacccta aaaacatcgc accagttgga aaaagaaggc gaaatctaca aaatggcgcg
    36301 ccagagcctg gagcggctgg cgaaggattt aacgtctctg cagatgtccg agggatcgtt
    36361 ctacctgaac gccgaaaaga aaaaaacagg acggcatcaa tttcacaaca tttttttctg
    36421 gtcggcagcc catttgactt tcggagaact ggaaggtgag gggcgaccgg caacgatcgg
    36481 atattacgtg acggaaaatc ctgatggaaa aagcttctcg ctctggagat ccgacgctac
    36541 cggaatgaaa ccggatgaga caaaaaaaac agaagacgga ttcatcatct gtaaaaacat
    36601 tgaaacattt catctgacgt tctacgattc ggccggggga gaaaatgaca catgggagac
    36661 gtccgccggt ggaggcatgc atcagggcaa agtacccgaa gcggtgaaga tagagcttgc
    36721 gctggtcaac gatgatgatg cgcagaagcc atacaaattc atgaccagag tattcttacc
    36781 ggtgaacaaa taaaatgatg aacatattcg ccagacatca ggatgatcaa aaaaagaatg
    36841 acccccaaaa gtcatttctt aaattttcta tcctcaaagg tttcatggcc ttccgaaaac
    36901 gccgggatgg ttcaacttac caatttccgt tcactgcgtt ccggaaattg ttgtttcaca
    36961 acccactgct gtcgcgggat ggttcaactt accaatttcc gttcactgcg ttccggaaat
    37021 tgttgtttcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    37081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna caaccctcga
    37141 ggggttgccc tgatcacggt cattttgatc attgtcattc tgattgccgt ggtgatcgag
    37201 ttgaacagat cgtcccgcgc cgatatttac gatgcagcca acttaagcga cggtataaaa
    37261 ttaagctata tcgccaagtc gggattttac ggagccgccg ccctattgaa caattccccg
    37321 ggcgcttaca cctctctgcg cgacgactgg gcgaaaatgg aaacggtatc tctgcagtcc
    37381 aatactctgt ttccggaagg gtattttatc gccagaatcg aagatgaagc gggaaaaatc
    37441 cctttgaaca agctggttga cggcgaagaa tacaatgaag aaatcagaac catgctcgac
    37501 cggttactgg cacagccgga attcggcctg aaggacagac aaatcgatga aatcatcgat
    37561 gccatcaaag actggatcga cacagatgag aacacgaccg gttacggagc ggaaagcaat
    37621 tactacgaat ctcttgattc tccctatcag gcaaaaaacg ctccgtttga ttgcattgaa
    37681 gaattgctta tggtaaaggg aataaccaaa gaaatctttt atggaacgaa agacaaacca
    37741 tcattggcaa aatatgttac cgcagacagc gaaggattga ttaacatcaa tacggcgccc
    37801 aaaatagttt tacgctctct cagcgatgac attagttccg agctggccga caggctcgat
    37861 gaataccgca tggagaagag ccatgatgtt tccggaaatc aatggtacaa acaggtgccc
    37921 ggtatggaaa acataaccat taatcaaaat ctgattacgg tcaaaagcac ttattttaaa
    37981 atcgtatcca cgggcaaaat cagcaatatg tcaagaaccg tgaccggcat cgtcgccacg
    38041 ggggaccagg ctcagattgc gatcatcacg tggaggcagg attaatgccg aatgttctgg
    38101 gactggaaat cggccggagc acaattaaag ccgttctgat ggcccgaaaa ggcttgggtg
    38161 gcggtcgtat tcttgacgcg cgcattctgg acgtgcgcga cagcgaagga attggaccgg
    38221 cactgcagaa attggcggaa gataaaaatt ttgcaggtat cccctgcttt gtgtctgtgc
    38281 cttcccagga cgtcctcttc cgtcatgttc gcctgccctt ccgcgatgat aaccgaatcc
    38341 gcaaaacgct gctcttcgaa ctcgaaccgc tcattcctct gcccattgaa aacgtcattg
    38401 cagactacat cgcccttccc cgcgaaggac tgctcgtcgg cgccatgccc aaagaaacca
    38461 tccgcgaaat tattgaacag gtggaaaaaa atctcggcga cgtctcggtt attgacacct
    38521 cttcttcggc gctggccgta cagataccgg atcataaaaa aacttccgtc agcatcattc
    38581 tcgatatcgg acgcaattcg accacagccg cattttgcga aaatgaatct gtgattcata
    38641 tccgttctct ggccttcagc ggcgaacaaa tcacgctggc gatggcaaaa gatttttccg
    38701 tggacaggaa tcgcgcggaa cagctgaaaa tcagcggcga ttatcccgag gctatggaaa
    38761 aggacgggga ggaatgccgg gggctttgtt cggaattgaa aaacaccgtc gaattcatga
    38821 agataaacgg cgttctgcac aatgatcctg cacaaatcct gctgacgggc ggcggggctc
    38881 tgttcggtcc gctgcgcaaa gagatcgaga aaaactttac ctgcccggtg gaacttctca
    38941 acctgatcag tgccagacaa ctggaagttg aaaaaaatat catgatgcgg atgaatccgt
    39001 ctttattgaa tgcggccgcg gccaacgcgc tgagaagttc ggccggcaaa aaatcattca
    39061 atttccgcca gggtgaattt accgcccggc cgtcgcatct gaatttcaaa aatcacctgc
    39121 gctgggcggg catccttgcg gccgtgatcc tgctgctggc cgtggtgaat caaattctcg
    39181 attacagcct gaaaatgcga caaattggcg gcatcaagaa ggacattgcc aggattttcc
    39241 aaaagagttt tccggacgct ccgtccatgg tggatccggt gcagcaattg aaaacaaaaa
    39301 tcgaagaaga taaaaaaaca ttcggtctcg gcgaaggaat gccggaagca acaacagccg
    39361 attttttaaa ggagctctca gcgttgatat cgccttccct ggaaatcgtc ttcactgcac
    39421 tgacgtatga aaacagggtc atctcggtaa aaggagaggc gaaaacggtc gatgatgtga
    39481 cgttgattaa agacgcgctg gccaaatcaa aatctttcaa agaggtgacc atggggccga
    39541 cgagtctgac ccgtgacggc ggcaaagttg atttcagttt aaggattgaa gtccaatgat
    39601 acaggaactg tggaacaaac tggatgccac acagcgcaaa cttgttgcgg gcgcggccgt
    39661 gttcgtctgt atcgcgctga ttctggaact ggccgttttc cccttctggg aagctaaaaa
    39721 gaaactggac agatccatca gcagcaacca gaaaaaactg gccgaggtca aagaactttc
    39781 cgccgagttc aatcggttgg aatcgaaagt gtccgccatg cgaagagtcg cttcgatgcg
    39841 cgcggcaaat tttacgctgt tttcccatct ggaaaagaaa gcgacgcagg ccggcgtcag
    39901 aaacagaatc aaaaacatga actcctccag aggcattcaa tccgcctcct ttgaggagtc
    39961 tttgatcgat atgaagctgg agaaggtcac catcaaacag ctcgttgatt tcctttatta
    40021 cgcggaatcg ccggcggatt tagtgagagt caaaaagatc catattgtaa aaatgaagga
    40081 cgctccggaa tatttaagtg tgcaattgca gatatcttcc attcagccgt cgaatcaaat
    40141 tccggaaagc gggcaaccat gaaaatactg aaaaacaaga tattctggtt tacgatttac
    40201 gggatattca tcacgctggt ctttctgtat ctgctctttc cctcggatat cgcccaaagc
    40261 aggctggaag aatccattgc ctcctccggt ttgattttga aaaccggctc attaaaaccg
    40321 tccctgccct tgggtctgaa aatgaaaaat gtccgtctgg gttcagggtc ctccccaaat
    40381 atttattttc agggagattc cctcgacgta cagtttcacc cgttgaattt tttccgggat
    40441 aaaaaaaata tccgcctgag cggaaaagca tacggcggaa ccttttcggg aaatttcaag
    40501 accacggcgg ccggttttca tccgccagag gaagggatgc tgaaattcaa gaacatcgcg
    40561 ctggaccggt acacgctcat caaagcgtta accggtaaac agataacagg caaggcgagc
    40621 ggtaactgga cgcaggcggc gggatcaggg ggacgtgaaa actcttccgc aaccatttcg
    40681 cttttcctca acaagggatc ttatccgctg gcggaaccct tcctgggact gcaacgaatc
    40741 gattttgacc gtggcgagat tcaggccagg ctggaaaacc gcgtcctgaa attcgaaaag
    40801 ttccagatct tcagcccgca aatggactgt tctttaaacg gcgacatcat gcttgttgac
    40861 gaattcaaaa acagcccggt aaatctaacg ggcgaaataa tcatatcggg tagaaaggta
    40921 aaaatgaata tcaccattga aggtaccatt gccaatcctt ctattcgcta tatgtaggta
    40981 agatgatgtt tcctgaaatt acagcgcaga tcaaagaacg tatcgggtct ttcaaaagaa
    41041 tgccctccgt ttcatctttg gataccggtg tcttgatctc tgtggccgtt gtactggtca
    41101 tcaccattct gtcttacgag gcgaccgacc tgttttataa agtggtccgt ttcccgctga
    41161 ccagacacac tgccgttatc aaaaaccatc atgaatcatt cgtcaggaac aaccggcagc
    41221 accagcagct tcaggattac gatatcatca ccgaacgcaa tctgtttttg acgacgttga
    41281 gaacagcagg cggaagcgaa tctgcggacg ggcctttcga tgccgaccag aaaagcatgg
    41341 atttcgatct gaaaggaacc atagccatta gttcctcttc cggttttatt atcgttgagg
    41401 aacggggaag caaaaaacag aagttatacc gcctggggga caagatcggc gcccgcaagc
    41461 tggctaaaat aacaagaaac accgccgttc tgaaaaacgg cgaggaagaa ataacagtaa
    41521 aaatcaaaga gacggtggaa gataatttgc tttcccctgc ccccggctcc aggaggaatc
    41581 cctcgccttc aagaaaccct gctctttcca gactgggaat tgatggaaac gcaaaatacc
    41641 tgcaatccat tatgaatcaa gcggttgttc gtcccttcat gagcaggggc gtgcgtcagg
    41701 gatacatcat ttccaatatc aagccgagta gcctttacga aaaagccggg cttcagaacg
    41761 gcgacatcat cgtcggaatc aacaataatc cgttgcacaa cgccaacgat gtcatgcaag
    41821 ttttaaatgc gatgcagtcc ggaagccgtt tggaactcac cgtcaagcga agaggacgga
    41881 cggaaacgat taattatacg ttggaatgat tgcctcggga gaaaaatatc catgtatgcc
    41941 gcgtattttg ggttgaaaga aaatcccttc agcttgtcgc cggaaccgcg ctaccttttt
    42001 ttaagcgaac agcaccgcga cgccttaaac tgcttgattt acggaatcaa agaaaaaaag
    42061 gggttcgttc tgctttcggg cgatatcggc atgggcaaaa ccacggtctg ccgcagtctg
    42121 ctggcctcac tggatgattc ggtggaaaca gcgctcattt tcaataccgc cgtttccgat
    42181 atcgatttac tggaaaccat tctcgaagaa tacggcgtcg acatcgaacg cgaatcccga
    42241 agcaaaaaaa attatatcga ccgcctgaac agttttctgc tggaaaattt ctccgccggc
    42301 aaaacagccg ttctgcttat cgatgaggcg caaaatctat cgcatgcggt gctggaacag
    42361 atccgcatgc tttccaatct ggaaaccgaa acggaaaaac tgattcagat tattctgatc
    42421 ggacaaccgg aaatcaataa ccatttgatg ctgcccgctc tgcggcagtt gaatgaaaga
    42481 atcacggtcc ggtatgcttt gaaaccgcta tcacctccgg aagtgcgcga atacattcat
    42541 catcgtctga aagttgctca cggcccgggg gacattcgtt tcaccggcgc tgcctacagg
    42601 ctcattttca atttttcgga aggcatcccg aggcggatta acacgatttg cgaccgggcg
    42661 ctcctgatcg cctataccaa aagcataacg aaaatagatc gcaaaatcgt caggctggca
    42721 gttcacgaca tcggcacaac ttattttcaa aaaaccatga gtctgggcag aaaattactg
    42781 gcgcgtctga ccgtctgacc gaagctggaa agtcttgacc aaagggatat tatgagctac
    42841 ataaacgacg cattacgcaa agcacagcag gacaaaaaat caccctatgc ggcatatgaa
    42901 ccgcttttgt cgccttccgg taaaaaaaat accgggcgga ggcgatggct ggccctgctg
    42961 gggtttttgg cattgttttt ttgtgtggcg gtggcaggac tttttgttct gatgtccggc
    43021 cctgaaacaa aaaaagccac ggccaaagcg ccgctgacga ttccgacggc accggtgatt
    43081 gaaacgccgg tgattgaggc accggttcaa ccggctgtgc aagacataaa ggttgaaaat
    43141 aaagtcgagc cggtattaaa aacgacagaa aaaaagactc aaaaaaccga agaaagcgct
    43201 gcattgaaaa tacggacagc cgattcagta aaactttacg ccgaagcgct gcggaaacat
    43261 caggaaggca acctggagca ggcaaaggat ttatataaga aagttattga gattgacccg
    43321 aagaatatag aagcgctgaa taatttgggt gtcgtttata tgaagaagaa aacttacaaa
    43381 tgggcaatta ttcgactcaa cgacgcgctg aaaataaaac atgattaccc cgatgcgcat
    43441 tacaatctgg cctgtatcta cgcgcaaaac aacgacctga gccgaagtct ttcctatttg
    43501 aaaaatgcta tcggctttaa ccctcaggtc agacagtggg cagaaaaaga caaagatctt
    43561 gaggttctgg ccgatttgcc ggaatttaag aaactattgg agaaaaatta acgtcaagat
    43621 gaaaaaatca catgttctta ttttgaaagc cggtttttta atatgggtca tgatcctgtt
    43681 ggtgattata agcggttttg ccggttcagc gctggccgcg cgtgtcgccg gcggcggtga
    43741 agagatcgag actacggcta tgccggatgc cgctccggaa ccggcgatag aagaagaggt
    43801 ggacccggcg ccggaacccg agctgatgaa tgaaccttcc cgtcagaatg ccgagatgga
    43861 cgcaccggtc atgtctacgg acaacaaaga tgaaaaacgg tcttacaagc ccattaaagt
    43921 caatccgtcg cagaaatatg tcacgctgga tttcgacaat gtggatgtca gcgtactggt
    43981 aaaattcgtc agtgaattga ccggtaaaaa tttcatcatt gacgacaaag tcaagggtaa
    44041 ggtcaccatc gtttccccca aaaagattcc cgtggaagat gtctataaag tttttctgtc
    44101 cgttctggaa attaacggtt tgaccgtggt gccctcgggc aatatgatca aaatcattcc
    44161 ctctgtgcag gcacgggaga aaggtctgga aaccaggaca aaacaggatc ccctgttgcc
    44221 ggaggatcgc atggttactc agatcattcc cctggagcgg gccaatcccg atgaagtgaa
    44281 aagaatcctg gatccgctta ttccgaaatc cagttctgtt ctttcctatc cgccggcagg
    44341 catgctgatc attaccgatt acctttccaa catcaaacgg attcaggaaa tcgcaaccgc
    44401 gctggatgtc gaaggcgccg gcgatcagat atcctatatc ccgctgcaga atgcttccgc
    44461 ttcggaagtg gtcaaatcgt tgaataccat cttcacacag ggtcccgtcc gcggaaaggc
    44521 cgcgacaatc ttaaaagtcg tagcggattc gcgcaccaat tcaatcattc tgctggccag
    44581 cggcgccgaa acagaaaatg tgcgcaagct gatcgcattt atggacaagg atgtgccgcg
    44641 cggagaatcc aatattcagg tttatcgttt acaaaacagt gtcgccgaag atctggcaaa
    44701 agtgctcacc agcattgtca aggaaggcgg ggcagcagcc ggcgccacag caacggcaac
    44761 cggagcgaaa acagtaccga cgactgtcgt ttccaaaaat gttcaaatcg ttcccgataa
    44821 agcgaccaac acgctggtcg tgatggcgga acgggaagac tacaagatta ttgaaaacat
    44881 catcaagcag ctcgatattc cgcgtccgat ggtctatatt gaagctttaa ttatggaagt
    44941 caacgccaac aaggatttca aactcggcgt cgaatggcgg ggcatcaccg aacatggctc
    45001 cgttgacgga aatcccacgg cggcttttgc cggttcggga ggcgccggcc cttccggtcc
    45061 cggttcctac aatatcatgg acggtttgct gacaacgacc ggaatgggcg tctttcccag
    45121 cggtttttcc atgggactgg tcggggcagg cattaagatc ggcggcgtca ccttccccag
    45181 catcggtgcg gtcgttcagg cctataaaac cgattcggaa gtttccatct tatccacccc
    45241 gcagatcatg acactggaca atgaagaggc ggaaatcaat gtggggtcga acgttcccta
    45301 tgtgacaaga caagatcaaa caaatgtggg caccggcaca aattatacga actacagcag
    45361 ctatgaatac agggatgtcg gcgtcatttt aaaaatcacg ccgaatatca atcaggaaaa
    45421 ctttatccgg ctgaagatcg atcagcaggt gaccaaggtg cagtcgggcg ccgatgaagc
    45481 aagaccgacc acgctgaaga gaacggcaaa gacaacggtg atcgtcaaag acaatgaaac
    45541 ggtcgtcatc ggcggtctgg tcggcgacag tacggacgac agcacgtaca aattccccct
    45601 gctgggcgac attcctcttc tgggctggct tttcaaaacg aaaacatcat cacgggaaaa
    45661 gaccaacctt tacgtatttc tgacaccgca catcgtccgt acgcaggccg atgccgcaaa
    45721 aatttatcag gaaaaaaggg agaccatggg agaagtggtc gagggcgtca tcaaactgaa
    45781 tgaaaaagcc gatgcgaagg ctccccaggt gcgtcctccc gttgaaaacg aaaccatttc
    45841 caatccgtaa gcctgagggt ccgattcgat atgtccaaaa tccatacagc tctgaaagaa
    45901 tcggcggcgt ctgctcatgg tgatgcatcg tccggcaaat ccgttttttc gtctctggat
    45961 gaaaagctca tcgccgcggg agtggcaccg gacaccctca gtgaaatacg aaccgcaccg
    46021 gcaaacaacg gcggaggatt tttcgatcaa ctgatcaaaa agaaagtgat tcctgaaatc
    46081 catcttttaa aaatcctttc cgaacatttc gggctgtcat tctggcagca actgcccatg
    46141 gaaagcattg atatcgactt tacccaacgc atccccattc aatatttaaa aaaacataaa
    46201 atcattccat tgatcaccgc cgaaaacccg gtgatcgcat taaacgatcc ttcgaatttc
    46261 cagcctgtcg atgacttgag acagctcctg aagaatccaa atctgtcggt tgttctgtct
    46321 ttgcaggacg caattatgtc ggccattaat atggcgtatg acatgagccg ctcatcggcc
    46381 aaggattatt ttgaagaaat ggaccagtcc tccaccgacg atctcatttc caaaatcgat
    46441 gaaaccgccg atctgctgga tgaagccagc gatgccccga tcatcaaact ggtcaacctc
    46501 attctttccg gcgcgatcaa ggaccgcgcc agcgatattc atgtcgagcc ctattcggca
    46561 aacctgaaaa tccgctaccg cattgacggc attttatatg atacactcag cctgccgagg
    46621 cgcatccagt cgccgctgat atcacgtatc aaaatcatgg ccaaactcaa tatcgcggaa
    46681 aaacgtctcc cccaggacgg acgcatcgaa ataaaaatag ccgatcgcct ggtggatatc
    46741 cgcgtgtcgg tcatccccac cgccttcggc gaaagagtcg ttctgcgatt gctggacaaa
    46801 acggccaaca ttttaatgtt atccgatctg ggcatgcatg atgaacggat caaactcctc
    46861 aataagctga ttaaatcgcc ttacggaatc attctggtga ccggccccac cggcagcggt
    46921 aaaacaacga cgttgtacgc agcgctttcc accattaacc gtccggagat caatatcatc
    46981 actatcgagg accctatcga atatcaaatg gacggcgtgg gacaaatcca ggtcaatccc
    47041 aaaatcgatc tgacgtttgc cgccggcctg cgttctatcg tccgtcagga tcctgatgtc
    47101 attctcatcg gggaaatccg tgaccgggaa accgctgaaa tcgccattca gtcctcgctg
    47161 accggacatc tggtcttctc cacgctgcat accaacgatg ccgccagtgc cgttacccgt
    47221 ctgatcgata tgggcattga accttttctg gtcacatcgt ctattgtcgc gatcatcgcc
    47281 cagcgactcg ttcgcgttct ctgcccccat tgtaaagagg tttatgagcc ggatgaagaa
    47341 tcgctggcca atctcggttt gagccgatcc gtcctgaaaa acaacacttt ttaccgcaag
    47401 aaaggctgca atctctgcat gcagacgggt ttccggggac gttcagccat tttcgaaatt
    47461 ctgaccgttg atgatgagat taaaaggctc gtactgaaaa catccgacgc caatcagatc
    47521 aatgaactgg ccatcaagcg gggcatgatt acattacagc aggatggtat cgacaaggtt
    47581 ctcgcaggca tcacgacaat cgaagaagtg ctgagagtta cccgcactct cagtcgtacc
    47641 gatgacatcg tcatcgaagt ttaatgaggc tctctgaact atagtgaaga gttagccgaa
    47701 ttatccccga agcgaagcgg agtgggatag tgagtctcgc tactggaaac ggaataaaac
    47761 atgatcgaat tctataaaat gagcggcagc ggcaatgatt tcatcatcat t
//
LOCUS       DAOOLB010000010        46080 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_560_length_46080_cov_40.696752, whole genome shotgun sequence.
ACCESSION   DAOOLB010000010 DAOOLB010000000
VERSION     DAOOLB010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 46080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 46080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46080
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_560_length_46080_cov_40.696752"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(225..992)
                     /locus_tag="PLF77_02485"
     CDS             complement(225..992)
                     /locus_tag="PLF77_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HPC07751.1"
                     /translation="MSFVMNTLAFVFGAAIGSFLNVCIFRLPAEKSIVTPASRCPYCL
                     HPIRFYDNIPIISFILLGGKCRDCESRIAWRYPLVELTMALLSVALFMKFGLSLNFFV
                     FFIFTAVLMVITFIDLDHQIIPDILTLPGIPLFFLAAVFIVRIPWLEAFLGFLIGGGV
                     LFVIALGYEFIAKREGMGGGDIKLLAMIGAFLGWKSLIFILLFSSLTGAVIGIAAMVI
                     HKQDTKYAIPFGPFLSAAAIAYIFFGQCYMNLFLMGY"
     regulatory      complement(1075..1165)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     assembly_gap    1182..1281
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1282..2319
                     /gene="ribD"
                     /locus_tag="PLF77_02490"
     CDS             <1282..2319
                     /gene="ribD"
                     /locus_tag="PLF77_02490"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HPC07752.1"
                     /translation="ARGCVSPNPMVGAVLVKNGRIIGKGYHQCFGGYHAEINAIQNAA
                     GRVGGSTLYVTLEPCCHEGKTPPCVDAIIHNKIARVVIGTIDSNPLVCREGVNRLIHS
                     GIDVKTGILEEECRLLNETFFHYMETGLPFVTIKYAQTLDGRIATSTGNSQWISSPAS
                     LKLSHRLRAEHDAILVGRQTVERDNPELTVRLVRGRNPLRVIVDSALKVSPDARIFQN
                     LSAAPTLVATLKTASDSRYKKLAALGVTMVTVSADRDGRVDLKKLFKKLAAKGISSVM
                     IEGGAQIITSVLKRNLANRLVTVIAPKIIGSGIEAIGDLNIRHLDLAMKITVKKISVR
                     GHDIIVDGRLT"
     gene            2428..2637
                     /locus_tag="PLF77_02495"
     CDS             2428..2637
                     /locus_tag="PLF77_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPC07753.1"
                     /translation="MKGYIVINKEICKECLLCINFCPKGHIVTTTEYNALSYHPVGID
                     ETKECNGCGICATMCPEVAIEVYRE"
     gene            2630..3688
                     /gene="vorB"
                     /locus_tag="PLF77_02500"
     CDS             2630..3688
                     /gene="vorB"
                     /locus_tag="PLF77_02500"
                     /inference="COORDINATES: protein motif:HMM:NF005507.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HPC07754.1"
                     /translation="MNKVLMSGNHVIGEAAIRAGCRFYAGYPITPQNELTEYMAEHIR
                     HAEGGIFIQSESEVAAINMIAGASAAGLRVMTSSSSPGISLKQEGISSMAACELPGVI
                     VNIMRGGPGLGNIRPSQGDYFQATRGGGHGDYRTIVLAPASGQELSDLTMEGFDLADK
                     YRNPVMILADGMMGQMMEPVLFKKPKPRKYSEKFMLRGAGTGKSKFIHGLILDPIDME
                     EHNWKLARKYEVITQKEPRFEEHKIEDAKMVIVAYGTAARIAKGAIKRLRAQGVKVGL
                     FRPVTLWPFPELRLRSLSEIVKHYLVFEMSTGQMLEDVRLSLQGYADIKFHGRPGGAV
                     PTPAELANVIAKFYEKID"
     gene            3709..4479
                     /locus_tag="PLF77_02505"
     CDS             3709..4479
                     /locus_tag="PLF77_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPC07755.1"
                     /translation="MVKTVFKRPRSLKENVFHYCPGCGHSIAHRIIAEVIDELDIRGI
                     TIGVPPAGCAVLAYNYFDVDMIEALHGRGPAMASAIKRSLPDRVVFSYQGDGDLAAIG
                     IAESFHAANRGENITIIFINNAVYGMTGGQMAPTTMLEQKTTTSPAGRHRTLDGYPVK
                     VSEIFSQLEGVTYLERCMVNSPLNINKTKKAIKKAFQCQLDGFGFSLIEILSPCPTNW
                     KMTAFNSCKWIDEVMSKTFPPGVYKDNIAAAKKSAEGR"
     gene            4479..5021
                     /locus_tag="PLF77_02510"
     CDS             4479..5021
                     /locus_tag="PLF77_02510"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPC07756.1"
                     /translation="MLIKTIFSGFGGQGVLMMGVSFAHSGMSQGYQVTYLPAYGAEMR
                     GGTANCTVAIADEDIASPVASEPDFLVAMNSPSLFTFQNKISSGGTIFLNSSIIKEKP
                     HRSDIKVCSMPCADMAQDLGNIRVSNTIMMGAFIKVSAVIPPDVYLKSLETIMGSKKK
                     SLAELNRKAFNAGYEYVKND"
     gene            5032..5463
                     /locus_tag="PLF77_02515"
     CDS             5032..5463
                     /locus_tag="PLF77_02515"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07757.1"
                     /translation="MPVKQISVILNNVPGSFAALTHILDLEDISTKAVSAASIADDSK
                     VRLVVNDPDRAAAVLKSYNLNFEVTPVLAVEVPLHAGGMNAILKPLAKAEINIHYLYT
                     TINRFGEETIVILGVNKIDEAREILEQNWIHLIDEEIYSIP"
     gene            5485..5892
                     /locus_tag="PLF77_02520"
     CDS             5485..5892
                     /locus_tag="PLF77_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HPC07758.1"
                     /translation="MPVSSEKEKMLQDRMKSLGVAETDLEETFIRSSGPGGQKVNKSS
                     SCVYLRHLPTGLSVKCQKERSQSLNRFLARRLLLDKIEIQQKGLMAEARQTIEKIRRQ
                     KRKRSKRAKEKILTDKHQQAEKKILRAKVSVEE"
     gene            complement(6054..6431)
                     /gene="atpE"
                     /locus_tag="PLF77_02525"
     CDS             complement(6054..6431)
                     /gene="atpE"
                     /locus_tag="PLF77_02525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPC07759.1"
                     /translation="MKKSLMYVMLAMVMVIVSAPFVFAAPEAAAQAPAGTVDYTKAIV
                     ASIFALAAGLAIGLGAIGAGLGLGQATSGASNAVGRNPEAQGKVMLTMMVGMAMTESV
                     AIYALVISLVILFANPLLKIIGM"
     gene            6629..7096
                     /locus_tag="PLF77_02530"
     CDS             6629..7096
                     /locus_tag="PLF77_02530"
                     /inference="COORDINATES: protein motif:HMM:NF037131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07760.1"
                     /translation="MRKLTSKQKKIARLLQKDIPVTKFPFKKLSGAPTPGAKEILTAL
                     DQFREQGLIRKFGAILRHQKAGYGQNALVLWSVPPSQTETTGRLFASFSFISHCYERH
                     PAFLERYNLFTMLHSDGRSISSLIKDMVNATGIGDYLILKSLKEYKKTSPEYF"
     gene            7096..>7757
                     /locus_tag="PLF77_02535"
     CDS             7096..>7757
                     /locus_tag="PLF77_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPC07761.1"
                     /translation="MKETTSVQFSKANRVIAGGVNSPVRAWKSVGGHPRFVLKAKGSR
                     IYDTLGKAYIDYVLSWGPMILGHAHPDVTAAVRAALKQGTSFGAPTLSETAIAEMISS
                     ALPSIERIRMTSSGTEAAMTAIRLARGYTGRKLIAKFDGCYHGHSDSVLIKAGSGPTT
                     LGIPGSPGVPDDIARLTLSLPYNDTNALAKTVKRYGSQLAAVIVEPVAGNMGVVAPRE
                     EFL"
     assembly_gap    7758..7857
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7858..8112
                     /locus_tag="PLF77_02540"
     CDS             <7858..8112
                     /locus_tag="PLF77_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPC07762.1"
                     /translation="KGIPVCINRVFSMFTVFFQEGPVVDLHTALQSDTNMYARFFHGM
                     LKNGIWLAPSQYEAGFLSFAHTEKDLQKTLEACAKTLRNL"
     gene            8185..9216
                     /gene="gmd"
                     /locus_tag="PLF77_02545"
     CDS             8185..9216
                     /gene="gmd"
                     /locus_tag="PLF77_02545"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HPC07763.1"
                     /translation="MKKAFLTGITGQDGSYLAEFLLHKGYEVHGLIRRSSTFNTERID
                     HLYHDFHDPEARLFLHYGDLSVSAQLMDLLHSVKPDEIYNLGAQSHVRVSFDMPEYTG
                     DVTALGTLRILEAIRKTGITTKFYQASSSEMFGAAPPPQHEKTVFQPRSPYAAAKVYS
                     YYITQNYRDAYGIFAANGILFNHESPRRGETFVTRKITRAATQIKLGLKDKLYLGNLE
                     AKRDWGFAGDYVEAMWLMLQQNTPDDFVIATGETHSVREFAEMVFQKLDLDYKRYVEI
                     DKRYFRPTEVDALLGDSTKAKKILNWQPRVSFENLVDMMIASDMELAQKEKTLLDAGF
                     VSNNQNFNH"
     gene            9227..10171
                     /locus_tag="PLF77_02550"
     CDS             9227..10171
                     /locus_tag="PLF77_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HPC07764.1"
                     /translation="MLPQKRITITGARGFLGTHLLKKFKSKGCRNIEIADLPEYNLTD
                     INAIRRMYEETKPDVVIHLAAKVGGIGFNQEKPAELFYDNLIMGAQLLHEAHIRKIEK
                     FVALGTVCAYPKFTPTPFREEDIWNGYPEETNAPYGLAKKMMLVQSQAYRQQYGFNSV
                     FLLPVNLYGPGDNFDSRSSHVIPALIKKCFDAIDRQSDQLEVWGTGSATREFFYVEDA
                     AEAIYLATLLYDKSDPVNLGSGFEISIKDLTTLIAELTGFSGRIVWDSSKPDGQPKRM
                     LDTSKALKEFGFIAKTDIRTGLTKTIDWYKKNRHLLKK"
     gene            10231..10515
                     /locus_tag="PLF77_02555"
     CDS             10231..10515
                     /locus_tag="PLF77_02555"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HPC07765.1"
                     /translation="MDKETRKYAMQMAFASSIGIAMILAVFGCLLLGSYLDKKFDSGH
                     VFTVVFLFIGLAAGFRNLYVLVQKNFTDEETIIKSLKSEPHRQRPAPKKD"
     gene            10475..10870
                     /locus_tag="PLF77_02560"
     CDS             10475..10870
                     /locus_tag="PLF77_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HPC07766.1"
                     /translation="MNLIVKDPLQKRIEITNWIILAVIFIPSLFLAPLKFSLGILLGG
                     FVSIVNFHWMARGLRGIFQNLQGNPKGTVMVKYYIRLAIMAVVLYLLIANNVVDIIGL
                     LIGLSVVIINILATMIIALAKKNFIEEVV"
     gene            10877..11548
                     /locus_tag="PLF77_02565"
     CDS             10877..11548
                     /locus_tag="PLF77_02565"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004481.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPC07767.1"
                     /translation="MEPLIFFHTQWLKDVGIDMTIVFNTLFVMALLAFFSFLATRRTE
                     VYPGKTQNVMEVIIGGFDGLLNEIMGHNGRKFFALISTLGLFILVSNLIGIVPGFESP
                     TSNINTNAAMALIVFLSTHVVGIKEHGLKYFKQFMGPVWWLTPLMLPIEIISHISRPL
                     SLTFRLFGNIKGEDIVLLVVLFLVPWFVPLPVFVLMVFTSVVQTLVFMLLAMMYIAGA
                     MEEGH"
     gene            11783..16051
                     /locus_tag="PLF77_02570"
     CDS             11783..16051
                     /locus_tag="PLF77_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPC07768.1"
                     /translation="MQTLGINIGSSSIKLILLEDDRIVWQKVEPHEGNFVHALEKTLS
                     ERSFPPGVLTLATGTEGRNLLNINSVIEPLCIEEALRHLNGNVNALVSLGGEDLVVYS
                     IDENQQIITSFSGNKCASGTGEFFKQQLGRMNMHLNDIHSIPEESRILKLSSRCSVFM
                     KSDCTHRLNKGEASTGDIVLSLSDVMATKVIDFIKKAKITGKKVLLVGGVTRNPHLIR
                     FIRQRMPENDFIVPDQAVYFEAYGAALLAGSMGTPLPALNDLFKTHMVQFKRFKSLKT
                     AEGKVHYLPSKKSKVIAGREYILGVDGGSTTTKACLIDMETSEITASFYGRTHGDPVK
                     ALKNCFLEMQKQIREDIGDRAIKITLAATTGSSRELLGVFLETPGVYNEIIAHAVGTT
                     FYQEDIDTIFEIGGQDAKYVYLKNKVPIDYAMNEACSAGTGSFLEESAQGDLNIHHAW
                     EIGDIALGASEPLKFGEHCSAFINSDVRKAIQQGASREDITAGIVTSIVSNYLNRVVG
                     NRTVGQNIVLQGGVAKNKAVPLAFAMLLDKNILVPPDPELMGCFGVGILARQKLHEGF
                     LSRRDYDIEKILSTEILHEKEFQCSACDNFCPIRVLNVNGRKYMFGGRCNKYANIRKK
                     KSFDESDVVDYIEQRNDLLFNQCAPRPADLIRKKDFVVGIPKCFSVYSHWPLYAWFFH
                     TLGVETVLSSDITHEGVARVESTYCFPAEIAHGAVQDIFNRNVDYIFLPHFRDMESYE
                     SNTPACFCPITQSLPYYIKKAFPEIPEGKYLAPVVSFMYGTQKASESFIAMAVRLGFD
                     QEDARLAFSVALEKQMECFRKFAELGKIAIENSRKSQRPVIAVLGRPYNAFTPDANMG
                     IPRKYTSRGFTVVPFDILPFEDQGIYPNMYWYYGQQDMKAAALLKNEDNIFVTYISNF
                     SCAPDSFMLHYLKWIMGMKPFLVLELDSHTADAGVDTRIEAFLDIIEGYRSKISDIRE
                     SRYDNGLRFINTSGEDIHIRNTLTGEKIPIKNNPRVKLLLSNMGRLSVEVLAATIRGL
                     GIHAETMPVPDIYTLQMARNHASGKECLPSHLVLGSTLKYLSSEKYRKDEIYILFVPT
                     TTGPCRTGQYFVFYENLFKDLRLENVVVFTLDADNSYNELGTEFSKYAWWAVLIGDYM
                     KDIETSLRTCAADPVKAVSDYNIIWQKLIDVAEKDVTQSLPVLKEISSSIAKIPLKKK
                     IDDCPKVLIVGEIYVRRDDFAVDELIRHFSRRGIIGKVSNVAEWVYYCDFVRRHELQK
                     RLKLVPWYKKFLAAEFSDIVKWKIEHVYKKKVEKNIRDILSTTGLVPHAPHNMDEIME
                     NTEKHFVNNELYSEISISSGVAATAMMDGYAGIVNISPFACLIGRVIEGVLTPWARER
                     KYPAISIEIDGNLFPPNVISKLEIFMLNVMRFRNQDETDHRTTL"
     gene            16063..16647
                     /locus_tag="PLF77_02575"
     CDS             16063..16647
                     /locus_tag="PLF77_02575"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HPC07769.1"
                     /translation="MQENVTGLILAGGKNSRMGVNKAFLEIDGKRLMDRTLSVYRRIF
                     PKIIIVTNDPLAYVEFSEAMIVTDIYKGKGSLGGIYTGLFYAASDDCFVAACDMPCLN
                     EDFIRYMIGRRRSYDIIVPELSEGFQALHAIYSRRCLAVMKKMILADRLKIAGLFKDM
                     HTLRLTEEDIRPFAGNTPLFTNINTPQDLQSFRK"
     gene            complement(16658..17284)
                     /locus_tag="PLF77_02580"
     CDS             complement(16658..17284)
                     /locus_tag="PLF77_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPC07770.1"
                     /translation="MKCRQNLRQKLNLKNGLLQALNTKYATFYFSKTMIILDVVPYGS
                     REIFRYAIRRRAGNTPAIDFSFMTNNLGKAWWAVSDTTHRNYLASQHVLKMAKRYTIT
                     DKSQRPKIVKIPAVKVSALNCLDIKKIDLASCDLDRVIKSAYRRQVKIHHPDLGGKAA
                     DFRKIHSAYKELLAWADHPVFVHRRGFPDKWFYSAETQKWVQPTPLSD"
     gene            complement(17428..18045)
                     /locus_tag="PLF77_02585"
     CDS             complement(17428..18045)
                     /locus_tag="PLF77_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HPC07771.1"
                     /translation="MIEKNVKTDFSSKISEPVIGAGTYVHPLAAVIGNVILGENIMVA
                     PTACIRGDEGQPLHVGDDSNVQDGVIIHALETELDGKPVEKNQRDVDGKKYAVYVGKR
                     VSLAHQVQIHGPAVVMDDTFVGMKTLIFKSFVGKHCVIEPGVTLMGVTVDDGRYVPAG
                     SVVKTQADADVLPVITPDYPMKEMNKGVLHVNKALAKGYLAAAKK"
     gene            complement(18091..18708)
                     /gene="coaE"
                     /locus_tag="PLF77_02590"
     CDS             complement(18091..18708)
                     /gene="coaE"
                     /locus_tag="PLF77_02590"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPC07772.1"
                     /translation="MLNVGLTGGIACGKSTVAKMFVEKGAHLIDFDKLAHRLQEPGQP
                     AWQKVVDYFGRQILRPDQKIDRVKLGRIVFADKKKLRVLNKLVHPPVYREWQVRLEKI
                     GRKEKHAIVLSDVPLLFEGKKTHLFDLTILVLSDPEDQICRLISRNRISGDDARARLK
                     SQMPIGKKTALADIVIDNRDSIARTKKTVDRVWRELVRLEKTKSA"
     gene            complement(18711..19487)
                     /locus_tag="PLF77_02595"
     CDS             complement(18711..19487)
                     /locus_tag="PLF77_02595"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011996181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPC07773.1"
                     /translation="MLLVIDVGNTNTVLGLFDGKNLIHDWRIRTEIDHTIDEYGVLIY
                     NLYQSSRKKEKPFKTVSAIIISCVVPPMLNILEPLCIKYFNVKPLIVGPGIKTGMPIF
                     YDNPKEVGADRIVNAVAAYNKYRKESIIVDFGTATTFDYVSPKGEYMGGCIAPGIVIS
                     SEALFEKASKLPRVEFSRPKSAITKDTVSAIQAGIMFGYAGLVDGIVNRIRTEVETKP
                     LVIATGGLANIIAPETKTIEKIEEMLTLDGLRFIYELNKK"
     gene            complement(19471..20283)
                     /locus_tag="PLF77_02600"
     CDS             complement(19471..20283)
                     /locus_tag="PLF77_02600"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPC07774.1"
                     /translation="MDWNIARLQETLGTGLFGGHLHFYPEIVSTNDEAVALAMAGAPE
                     GTAVLADAQTAGRGRWQRSWISPPGVNIYTTIVLRPECESSGASQIPIMAGVAAAEVL
                     DGVCPGRVKLKWPNDVIIGPKKVCGILSQAKITSKKIDFAVVGIGINVNAGLHHFPKT
                     IRESATSLLIESGGEISRHDLLISLYENLGKWYKQLLQKGFEPIRKKWLESAAMIGRP
                     IRIAFKGESTEGIAKGLGDDGCLILVVEGNQEIKVSAGDATIMRWKDNAFGD"
     gene            complement(20301..21137)
                     /gene="nadC"
                     /locus_tag="PLF77_02605"
     CDS             complement(20301..21137)
                     /gene="nadC"
                     /locus_tag="PLF77_02605"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPC07775.1"
                     /translation="MEQRIVRIIQNALAEDIGSGDITTGATVNARKKGRAVAIAKGNF
                     IVAGTDVFAGTFRTLDKNINVQKRIPEGYPVKKGDIIAEVSGRLDHILMAERVALNLF
                     QRMCGIATLTHRYVRAVRKTKARILDTRKTVPGLRVLDKMAVKTGGGQNHRFGLYDGV
                     LIKDNHIEAAGGITAAVKAQRKKLSRFMKIEVETRNLDEVREALECGADIIMLDNMSV
                     SAMKRAVSFVAGRALLEASGNVGLKNVTDIAATGVDFISVGELTHSVQAADISLKIRS
                     SS"
     gene            complement(21137..>21592)
                     /locus_tag="PLF77_02610"
     CDS             complement(21137..>21592)
                     /locus_tag="PLF77_02610"
                     /inference="COORDINATES: protein motif:HMM:NF021941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPC07776.1"
                     /translation="MITAVRNIRGEMRIAPSQKLDVLMSAVDEKTKKMVEKHKGYILN
                     LANLESLTVEINAVEPKGVATAVVGSTKLFVPIVGIVDLSGEIARLEKELAKISKDLQ
                     QSSKKLANRDFMSKAAPDIIQKEEDKVKDFQKKHTTLENALKKLKGMSA"
     assembly_gap    21593..21692
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21693..23587)
                     /locus_tag="PLF77_02615"
     CDS             complement(<21693..23587)
                     /locus_tag="PLF77_02615"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPC07777.1"
                     /translation="MAKRELGKAFEPAAAEKRLYEYWLKNKYFHAQDKSEAEAFSIVI
                     PPPNVTGMLHMGHALNNTLQDILIRFKRMQGFNTLWMPGTDHAGIATQNVVEQQLLKE
                     GTSRHDLGREKFIDRVWEWRRKYGGVIINQLKRLGCSCDWERERFTMDEGLSHAVREV
                     FVRLYNEGMIYQGDYIVNWCPRCHTAISDLEVEFKSDASFLWNIRYPFAEGQGEIVVA
                     TTRPETMLGDTAVAVHPDDKRYKNLIGKYVILPLMNKKIPVIADDYVTMDFGSGAVKI
                     TPASDPNDFAMAQRHHLEIITVIDGKGIINENGGAYKGQDRYEARKNIVNDLQKGGYL
                     VGTEPYAHNVGQCYRCKTDIEPMVSKQWFVKIKPLAKQATAAVIKGKTKIVPKTWEAT
                     YFDWMNNIRDWCISRQLWWGHRIPVWYCDNCKKVIVSTVDPDTCPDCGGASLKQDEDV
                     LDTWFSSALWPFSTLGWPEKTAALKTFYPTSLLITGFDILFFWVARMMMMGLYVMKDV
                     PFKHVYLHALVRDEKGEKMSKSKGNIIDPLLMIDQYGTDAFRFTLAAYTAQGRDVRLS
                     PERIEGYKFFVNKIWNASKLTFANLDGFDETATAEVVDSLPDLWIKARLNKAVEDVTT
                     ALDEYRF"
     gene            complement(23679..>24596)
                     /locus_tag="PLF77_02620"
     CDS             complement(23679..>24596)
                     /locus_tag="PLF77_02620"
                     /inference="COORDINATES: protein motif:HMM:NF012613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HPC07778.1"
                     /translation="LFVNTAAIRYHTAKKSDEKWLEKAKYYFVETVMIDSQYAPVYYF
                     MGIAYQEALEFDQAAKMFNKVIQLKAGHIEDANNRLEFLELVHKAHPATQAGKQIALK
                     ETVSRADIAMMLAEELKIKELYQKYLPKTAQEAQTEEEKVAASMSQAEHVAKEMEKMD
                     QFHSYMVLQEKEPVKLLANDIALHPLKNCIEAVLEAKVHGLENDPKGNFKPDEILSRG
                     EFAIILEDILIKVSGERYIAARYVSSNSLFPDVPADMPYFTPIISVTSRGIMEAKNTK
                     TGEFSPLKPMSGVEALLAVKKLKENLKID"
     assembly_gap    24597..24696
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<24697..25312)
                     /locus_tag="PLF77_02625"
     CDS             complement(<24697..25312)
                     /locus_tag="PLF77_02625"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HPC07779.1"
                     /translation="MTRRDHHTSVEYLCRLQRLFVVVLIALPLNIGAATHYHCADRSG
                     LQTFSDYPLEDQVCTEMVSQEETEIPKTDRPIAPNAESPEKKTTRIAVKENGILVPVT
                     LGYDGREADIRLVLDTGASATTIYDNVAGRLLVNLNNAPKAQGRVVGGGTIDANRVTF
                     SYLRIGPNTFRDWNIFVVENKDGDTSYDGLLGMDVLSTMRYKIDF"
     gene            complement(25309..26244)
                     /gene="miaA"
                     /locus_tag="PLF77_02630"
     CDS             complement(25309..26244)
                     /gene="miaA"
                     /locus_tag="PLF77_02630"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPC07780.1"
                     /translation="MKKLPLVIINSPTATGKTDLAVYLALECGGEIISADSMQVYRYL
                     DIGTAKPTMEQRRGIRHHLIDVVNPDGEFNAALFAARARAVIEELVHQGKPVFVVGGT
                     GLYIRALLKGIIDTPDVDASIRNHYRSLRETHGKIYLYDLLRQKDSTAAAQINPNDSV
                     RVIRALEVLEQTGESITVKQKKHSFSEDRYTTCKIGLRVERGELKERIEKRTDAMIRA
                     GLLDEVKGVLARGYRENLKSLQSLGYKQIIGFLNWQYDWRHCVNFIKRDTWQYARRQM
                     TWFSADKEINWFAPDALDILRRHVKIFQNENQETE"
     gene            complement(26256..28049)
                     /gene="mutL"
                     /locus_tag="PLF77_02635"
     CDS             complement(26256..28049)
                     /gene="mutL"
                     /locus_tag="PLF77_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPC07781.1"
                     /translation="MSKRIIVLSEDIANKIAAGEVVERPASIVKELLENAIDAGATDI
                     RVVLEKGGTQSIRVTDNGSGIDHDDVPLVFERHATSKIHQFEDIFNVVSFGFRGEAMP
                     SIASVARVEMLTRRKNDVSGTRAILDGGVIRAMDPAGTPEGTQISVSGIFAHVPARRK
                     FLKTEATEQGACLDVITRLALAHPEIRFKVEANGKEIFAAPEVHDTAKRIAMVMGGEF
                     SAHCMAVDAQQDAMTLKGFISRPEYSKSNSKSIYLFVNKRFIRDTSLTHAVLSAYRQI
                     IESRRYPAAALFLEVPPEDVDVNVHPAKMEVRFKNAHGVYELVSKTIAHHLSRAETSR
                     GHYLYRLAPAEKKDYFHRQPFVNEPSPSMPSRPADRSHAQQGIQNILLHRSQVSQPLA
                     ASEGDAGVPDKTILFGDMRYVGQYDGTYLLFEGPDSLILLDQHAAHERIILEKLKKDS
                     GKKAAGQSLLLPEIVNLTPGQMALLNDALDFLETIGLEIECFGRDAVAVKTVPTMLSK
                     VKISEMIYDIVDQLADQNRTPSLQERSDNLLASLACKAAIKANRLLSRDEVDALCREL
                     AETPFHATCPHGRPITIQFSLSEIERLFKRK"
     gene            28217..29599
                     /locus_tag="PLF77_02640"
     CDS             28217..29599
                     /locus_tag="PLF77_02640"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPC07782.1"
                     /translation="MEKFFDPHSVVVVGASNTPFNLGATICNMLKDYLQYPGAVYAVN
                     SKGEKVNGYPGYSSILDLPESPDLAVIIVAAKNVPRIIGDCARKGIKRVIIESAGFSE
                     GGSDGKAMQRKIDEIAKAHGIRIMGPNCLGTLDTRKKFCCFYGVNPSLVEMGRIFEAP
                     GNISFIIQSGGVVVLVIESFFYDLVGVNKVVSIGNKCDVDEADLIDYFQTDETQVIGL
                     YLESVTNGRKLMEAARKSRKPILIYKVGRTQEGALAAMSHTAGMANNDSIFNSACKQS
                     GMIRLQSIDELHSMPKMLTEMPLLKGKNIAMFTNSGAFGSIGADLLVDAHLQVVRLKP
                     ETQEKLKKAGQVFNIKNPVDIGPALPQTYLDIYEILLSADEVDGLLPLLSVWQTFVID
                     TLLELMKMCRHYQKPAAIYTPNAIAKSIGIRAKHRIPIFETAEQAVRALAVSGYHYDS
                     LQKRFPARAK"
     gene            complement(29629..30186)
                     /locus_tag="PLF77_02645"
     CDS             complement(29629..30186)
                     /locus_tag="PLF77_02645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPC07783.1"
                     /translation="MKLVIRGGSIAAGYGVTKSYVDILTQALLPEGVCVINRARYRET
                     SFDGIETYPQDIDRYEPDFLLIHFGVDDAFQCVYRSEFQENIVQMIRLSRKRLQPVIF
                     LATSHTFDNPNDMDAVHIFYRSLRIVASDLKCEFIPVHRSWNAYLEEHNLCSQDLTQE
                     DSRYPNEQGHQVIANAMMNVLGKFF"
     gene            complement(30248..31741)
                     /locus_tag="PLF77_02650"
     CDS             complement(30248..31741)
                     /locus_tag="PLF77_02650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC07784.1"
                     /translation="MKIVLLSMPDSIPMIMHESAFHLPNCGIASIGANLDEGHDVYVV
                     DLIRKRRHLAGYLKRLLLKIHPKLVGLSAMAWQYETCIEIIRFIRLLLPEVKIAIGGY
                     HATLMYEEIAASPESGLIDFMIRGEGEEAFRRLVNALEGSDQLQDIPSLSFKRRGTFC
                     HNPRGALLDLSRLKIPVRDKRRLTSGYHAVFSKIEVIETSRGCTRTCSFCSMREMYGQ
                     TFRTFPIERVTADIDDIYYKHNTRAIFIVDDNLVLDPERVIDLCDAIIAKNYKGLSLH
                     VQADCISIAGNETMVRKMSLAGVKTVFLGIENVVKKNLMASGKGDIVDLSRKAVRICH
                     QFGIMVIAGLIFGFPDDDENDIVDNYRFLKSLDADIPYCQILTPYPKTPVRQQLLERG
                     MVTNLHDYKLYNGLRANIKTRHLDTQQLQYLVWLHRQKMFGWWEPSRLMRIKGGLWVT
                     FWTHLLRPFLRSTVGRQQAQKSWKELYERDVKYLDGLNQFKNLARNY"
     gene            complement(31860..32123)
                     /locus_tag="PLF77_02655"
     CDS             complement(31860..32123)
                     /locus_tag="PLF77_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07785.1"
                     /translation="MQVNLTEQEIVKFAYVTKEIIEQSWGATLSNAQINCYIECFYTL
                     KDLLEQIDREYLQKHSEISDYFLTGKRLINEKDKLQWEKEWQV"
     gene            complement(32186..33115)
                     /locus_tag="PLF77_02660"
     CDS             complement(32186..33115)
                     /locus_tag="PLF77_02660"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC07786.1"
                     /translation="MKKAMLVHLNELDFYLKKIDAVWPVRCLTGEAIDCEKVVDYYRQ
                     SRIVYKYVHSYSGAMHMAVNSDGVFNAKGFYTSLNEIVEIISPLPAGAVLELGCGKGF
                     NSAYLAKKIPHAHFTGIDVSGEHLAIARKKASRIHNLSILYGDFQDIMFPDGAFDTVF
                     ELESICHARKVRQVLSEVFRVLKKGGRFIVYDGFRQSGFHRLPDRLIEAARMTEKALA
                     VDHFEDIDGWLAMAVAEGFRIHEKKDLSASIMPNLARLQYLARRYFEYPMLTKLLLRL
                     LNVYTMRNCIAVLLMPFTLHNNAHGYYKIILEK"
     gene            complement(33407..34318)
                     /gene="rfbD"
                     /locus_tag="PLF77_02665"
     CDS             complement(33407..34318)
                     /gene="rfbD"
                     /locus_tag="PLF77_02665"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPC07787.1"
                     /translation="MRILLLGHKGMLGNDLFLRLSAEHELIGMDQEDIDIVSDNDCCQ
                     AIKDVSPDMVINAAAYTNVDGCETDRETCFAVNAEAVKNIAGACRQKKIRVLHFSTDY
                     VFDGTARKPYREDALCNPVNVYGASKLAGENYLRTLSDNYLLIRSSWLYGINGKNFVQ
                     AILDKAKAKEFIRDTMNRAKAVAEAPSTLSVVDDQVGSPTYTKDLALAVAFLIRKNAT
                     GIVHVTNRGSCSWHQFAVRILNSAGYGHIVVEPIKSSELQRPAMRPAYSILSNKTFIE
                     ITGKAMQPWQLALEDYLNNFKLKKSHS"
     gene            complement(34321..35058)
                     /gene="truA"
                     /locus_tag="PLF77_02670"
     CDS             complement(34321..35058)
                     /gene="truA"
                     /locus_tag="PLF77_02670"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HPC07788.1"
                     /translation="MRNFKITVEYDGAAYCGWQRQHNGIAIQQLLEEAIKKITGQNVS
                     VIGSGRTDAGVHAMNQVGSFKCDTTLPVQKIFMGVNSVLPPDIVVKNLEERSDDFHAL
                     RDVKSKVYVYRICNQRLRPVLGRNYFWHVRYPLDIASMRKAVRLIIGTHDFSCFCAAG
                     TDVKDRVRTIVDIEIMTGEEGLIEIQVESHGFLKYMVRNIIGTLIEVGRGKRQPEEMK
                     LIIESKNRNIAGATAPACGLFLKEVKY"
     gene            complement(35097..37199)
                     /locus_tag="PLF77_02675"
     CDS             complement(35097..37199)
                     /locus_tag="PLF77_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPC07789.1"
                     /translation="MEKLFYPESIAIIGLSSKPQNIPRLVLENLIRWGYKGRIFGINP
                     RAADSHVDGIKMYKSVQELPVVPDLVYVLIPARMIPDVMDSCGRAGVKRLAIPTGGFN
                     ELGEEGRKLSELVLQKAREYGIRFVGPNGVTVANTANGLCLPFVPSYSPPKGGLSIIS
                     QSGGVGLMLWNLMTDENVGMAKFASIGNKLDLDEVDFLEYFGRDPETKVIGMYLESVP
                     RGDRLIAAASKIDKPVIVLKSNTTAAGRKAAMSHTAALANNEDIIDAAFERSGIIRIH
                     QFNDFISMAKVFELPPMRGKRMMVMSPAGGFTVMMADLCEASGFEFADPGADFYDKLK
                     NSSNAGVINFSNPLDMGDIYDMNAYPQIFFSVMHNENVDGAVFVTQWPEMPRGEDVFY
                     KMFHTDLSKEVAGTILSSGKPLGVCLFGLSATISRIKQNLDFPIFNNASEMVHAFKKQ
                     YDYYAKKARGETGYPLPDGVDIEAAQKWIKGHAGVRGEESLELLSRFGIHVAPSDVAK
                     NGKEVLALADTIGYPVVLKVVSPDAVHKSEAGGVITDVQNAEEAVKAFDQIRSNLFRY
                     NAHADFQGVRVMKQASDGYDMFIGGKFDESFGPVVFFGYGGIYIEVFQDTANLLCPAD
                     CHEIEAGVKRLKSYKILQGMRGKGAGDIAGYVAMIERVSHLLHQFPQIRELDINPVRV
                     LADGSGVLALDARVRIDT"
     gene            37532..38065
                     /locus_tag="PLF77_02680"
     CDS             37532..38065
                     /locus_tag="PLF77_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC07790.1"
                     /translation="MSFKKPYPAQYEKLLALKNGKNVFVRPIRDTDGKLLLELFDKLY
                     PDSIYRRFLTPLRMIPENLLFKLTHVNYDSDFALVAVIPENGKDAIIAVARYGYDVRE
                     QVTDFAILVRDDWQGLGLGKRLLKDIFAIGRDHGVSCFVSLIDSNNHVMKELIKRLDY
                     PVTYSYRTGASKVQIFV"
     gene            38225..39796
                     /locus_tag="PLF77_02685"
     CDS             38225..39796
                     /locus_tag="PLF77_02685"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPC07791.1"
                     /translation="MSEDVQKNSTQEKINHFEKKNRTLKQMGGEKQIARQHELKKLTA
                     RERLDLFFDQDTFQEVQLFVEHRSTLFGLDKKDIPADGVVTGFGKVNGRTVFVAAQDF
                     TASGGSLGEMHAKKIWKVMEMALAAQKPFVSLNDSGGARIQEGVPSLEGYGGIFYRNT
                     LASGYIPQITAIMGPTAGGAVYSPALSDWVFMVKGSSFMYITGPDVVKAVIGEEISHD
                     ELGGALAHAAKSGVCHVVTESDTDCIEKIKKLLAYLPDSCHAPLPLSDCTDPADRTCP
                     ELDSMIPDKSNRAYDMKKIIKSVADNGELFELLELWAQNIITAFIRVTGRPVGVIANN
                     PRFAAGVLNVDASDKAARFIRFCDAFNIPLLTFTDVPGYMPGTHQEWAGIIRHGAKLL
                     HAYSEATVPKITVITRKAYGGAYIGMCSKQLGADYVMAWPTAEIAVMGAEGACNIIYR
                     NDIAGADNPTARRAQLVEAYEQQFNNPYFAASMGIIEEIIPPRDTRKKIIALLDALGN
                     KKVTGLSKKHNNIPL"
     gene            39842..40957
                     /locus_tag="PLF77_02690"
     CDS             39842..40957
                     /locus_tag="PLF77_02690"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HPC07792.1"
                     /translation="MKKIFAAVALIVVLLTAYLTLWPVPVDPVQWIPPLSDGYSGPHA
                     VNTGLAGLRMISLGREEGPEHIVIGKDQKLYTTVAGGKILRMNQDGTQQEVYADTGGP
                     VLGFDFDARGNIVAADVDRGLVLISPDKNITVLTDNVNGDPIRFADAVAVSQSGKIYF
                     TDASTRFAPKKWGGIFPASLIDILEQRSTGRVLEYDPAAQTTRIVAKGFSFANGVVLS
                     EDENWLFVNETGKYRVWKISVTAQDLDIASPGESAVILFENLPGYPDNLMRGLHGKIW
                     LGFAKPRNPAIDQRVITSTPFMRKLTYRLPKFLWPLPKPYGHVLAFTEDGRIVADLQD
                     PGGRYPETTGVTETADRLYIQSLHAKGIGWLPKDNVF"
     gene            40947..41339
                     /locus_tag="PLF77_02695"
     CDS             40947..41339
                     /locus_tag="PLF77_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HPC07793.1"
                     /translation="MSFNLSRNPVYHILADILVAAHFLFILFVVFGGLLVIRKPSFAW
                     VHLPAALWGAVVEIFGWICPLTPLENHLRTLAGESVYGGDFIARYLLSLIYPENLTPS
                     IQKGLGAVVIVINLIFYFIAFKKNRARR"
     gene            complement(41336..42772)
                     /gene="gltX"
                     /locus_tag="PLF77_02700"
     CDS             complement(41336..42772)
                     /gene="gltX"
                     /locus_tag="PLF77_02700"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPC07794.1"
                     /translation="MRKPRVRFAPSPTGELHVGNARTALFNWMFARHHGGSFVLRIED
                     TDASRSALSYQTNLYDDLKWLGLDWDEGPEKGGHFGPYRQSERVAVYREFLQKLMKAG
                     AVYPCYCTEEELEEERQTLILSKRMPRYMGKCRNLSPEERNRKEKEGRKPSYRFRVQS
                     QIIEFEDLIRGNVKFNAEDMGDFIIVRSNGMPAYNFACVIDDHLMDMTHVIRGEDHLS
                     NTALQIMLYRAFGFDSPVFAHHSLILGRDRTKLSKRHGSVSIGEFRRQGILPEAMINY
                     LGLLGSSFTGAREVLSRDDMIQDFSLERASRSGAVFDEEKLHWLNAIYIRNTRTDDLV
                     ERLKPFLDEPAYQNEMQNPEWFRKAVELVKTDLTTLADLKNHISIFFDSQYRISAEAK
                     DLLDQEPARKVVEAFASYLEKQDAGNQNLYAEAVRFAREATGAKGRALFMPVRAALTG
                     LLHGPELDKIFDVLGRNAALRRLKYFER"
     gene            complement(42759..43949)
                     /locus_tag="PLF77_02705"
     CDS             complement(42759..43949)
                     /locus_tag="PLF77_02705"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF006899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HPC07795.1"
                     /translation="MKTVLIIPAGGAGQRFKSHVAKQYLLLDALPVLVHTLKVFQDSP
                     VVDEIILVLPQDDVVHVRQDLIEKYGMSKVVAVVAGGRERQDSVKNGLDAIQGNAGLI
                     IVHDAVRPFVTDQMIGRVVAAARACGAAVTGVQVKDTVKATKDDHMIQSTVPRENLWL
                     TQTPQAFRFDILKDAYRRAYEENFYGTDDAALVERTGKEVKMVEGSYDNIKITTREDM
                     LVAEAFMKKRNTAGIRSGFGYDSHRFTEGRKLVLGGVEIPCDRGLEGHSDADALIHAI
                     CDALLGAAGAGDIGRHFPDTDPQYKDISSMILLKNVKKILDGKGFRIQNIDTTVILEV
                     PRLAPYADAIVANIASVLNISEECVNIKAKTNEGMGFTGRREGVAVFAVASLAERMRN
                     SAKT"
     gene            complement(44069..45070)
                     /locus_tag="PLF77_02710"
     CDS             complement(44069..45070)
                     /locus_tag="PLF77_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HPC07796.1"
                     /translation="MKRLIIRIILIMACSISGYAIAFYTSSDSFLILKSIAGFIIGLT
                     IALTVIKIEQHIRQLSLKIIIGGVAGMIVGLLIALIFSFGLNFVSKIRENQQALPWIY
                     LLITGIFSYLGLTLGSKKIEEFSFGGIDSKTAHDHRILDTSVIIEGRIADICDSGFIE
                     GELIVPRFVLNELQQVADSSDSMKRLRGRRGLDVLNHLQKSANVNIEIVDQDFPKIKG
                     VDGKLVALAKQLNAKLVTNDYNLNKVAELQGIRVLNINELANAMKPVVLPGEQMTVKI
                     VREGKEAGQGVAYLDDGTMIIVDNAQKMLNMNVRVVVTSVLQTTAGRMIFSELKEVA"
     gene            complement(45233..45718)
                     /locus_tag="PLF77_02715"
     CDS             complement(45233..45718)
                     /locus_tag="PLF77_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HPC07797.1"
                     /translation="MFKVGDLAVYPAHGVGVIEAIENKEVMGKKQSFYVMKIMGNGMK
                     IMIPTMSVKAVGLREIIMEKEIPKVYEILRNKDVTIDKQTWNKRYREYLERIKTGSVF
                     EIAGVLRDLFILKSDKNLSFGERKMMDTAKNLLVKEISVATNAQESEVEHNLQKIFTV
                     Q"
     gene            complement(45942..46017)
                     /locus_tag="PLF77_02720"
     tRNA            complement(45942..46017)
                     /locus_tag="PLF77_02720"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45982..45984),aa:Cys,seq:gca)
BASE COUNT        11346 a        11818 c        10790 g        11726 t
ORIGIN      
        1 tttttaacgt tttcatcata aaatgcatct tcaaaataat tacagcaatt gatatgccat
       61 tttcctgaaa ttctttatga tttcaatatt ccgccctctc gattcgtatt tcctgatgta
      121 ttatcaatta ctacaacatc tcccgggcaa tttccagata taattcttaa tatcaatacg
      181 ttgtcttttt caccatcttt gggccttttt cgctttttta gaaactaata acccattaaa
      241 aataaattca tgtaacattg gccgaagaaa atataggcaa tggcggcggc ggataaaaac
      301 ggaccgaagg ggatcgcgta ttttgtatcc tgtttatgaa tgaccattgc tgcgataccg
      361 atgacggccc ccgtcagcga gctgaaaaga agaatgaata tcagggattt ccagcccaga
      421 aatgctccga tcatggccaa tagcttgatg tcgccgccgc ccatgccttc gcgttttgca
      481 ataaattcat aacccagcgc aatgacaaaa agcacgccgc cgccgattaa aaatccgaga
      541 aatgcttcca gccacgggat tctcacgata aatacggccg ccagaaaaaa cagcggaatt
      601 cccggcaaag tcagtatgtc cggaataatc tgatgatcga gatcgatgaa ggtaatgacc
      661 atcaaaacgg ctgtaaaaat gaaaaagaca aagaaattta atgataaacc gaatttcata
      721 aataaagcaa cggacagcag tgccatcgtg agctccacca gcggataacg ccaggcaatt
      781 ctgctttcgc agtcacggca tttgccgccc agcaagatga agctgataat gggaatatta
      841 tcatagaaac gaatcgggtg cagacaatag ggacatcttg aggcgggtgt gacgatggac
      901 ttttcagccg gcagcctgaa gatacagacg ttcagaaaac tgccgatcgc agctccgaaa
      961 acaaaagcca gagtgttcat tacgaaactc atgatgcttc ctgttcatgc atgtttttta
     1021 gtcctaacat tctcagggtg gtttttatga aacggctgga cttgcgggaa gttgccaggc
     1081 ggttctgcca ccctatcctc ttattgaaga cttaggtcag taactttgcg tcccgtcttt
     1141 tcagtacggt ttgccctttt caagctttgt attattttaa gnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn ngcgcggggt tgcgtcagcc cgaatcccat ggtcggggct
     1321 gtcctcgtta aaaacggacg gatcatcggt aaaggctatc atcagtgctt tggcggatat
     1381 catgccgaaa tcaacgcgat tcaaaatgcc gcaggccgcg ttggaggttc gaccctttac
     1441 gtgacgctgg agccctgttg tcatgaaggg aaaacaccgc cctgcgttga cgcaatcatt
     1501 cataataaaa tcgcccgtgt tgttatcgga acaatcgatt ccaatcccct ggtgtgccgc
     1561 gaaggcgtga atcgtcttat ccacagcggc atcgacgtca agacgggcat tctggaagaa
     1621 gaatgccgtc tgctcaatga aacttttttt cattacatgg aaacgggact gccctttgtt
     1681 accattaaat acgctcaaac gctggatgga cgcattgcaa cctcaacggg aaattctcag
     1741 tggatcagtt cgccggcgtc gctgaaatta tcgcaccgcc tcagagccga acacgacgcc
     1801 attctggtgg gcagacaaac ggtagaaagg gacaatcccg aactcacagt tcgtcttgtc
     1861 cgcggcagga atccccttcg ggtgattgtt gattcggcat taaaagtatc tcccgatgcc
     1921 agaatctttc agaatctctc tgcggcgcca acgctggtcg caaccctgaa aacagcgtcc
     1981 gattcccgat ataaaaaact ggccgccctg ggcgtgacaa tggtaaccgt cagcgccgac
     2041 agagacggcc gtgtcgatct gaaaaaactc tttaaaaaac tggccgcaaa gggtatttca
     2101 tccgtgatga tcgaaggcgg cgcccagatc atcacatccg tcttaaaacg aaatctcgcc
     2161 aatcgcctgg tcacggtcat tgcgcccaaa ataatcggca gcggcattga agcgatcggc
     2221 gacctgaata tacggcatct ggacttggca atgaaaatca ccgtcaagaa aatatccgtg
     2281 cgcggacatg acatcatcgt tgacggtcgt ctgacgtagc ggatcgattt tttttaattt
     2341 acgaacgaga ttgctttaaa aaacttgact ttctcgaaat tatacgcaag atagctggcg
     2401 caaatttaat ttttcaggga acggactttg aagggatata tcgtcattaa caaggaaatc
     2461 tgcaaggaat gcctcttgtg tatcaatttt tgtcccaagg gtcatatcgt gaccaccacg
     2521 gaatataatg ccctgagcta tcatccggtc ggcatcgatg agacaaagga atgcaacggc
     2581 tgtggaatct gcgccaccat gtgtcctgaa gtcgcaattg aggtatatcg tgaataaagt
     2641 attaatgtcc ggaaatcacg tcatcggcga agccgcaatc cgtgcgggat gccgtttcta
     2701 tgccgggtac ccgattaccc cgcaaaacga acttaccgaa tatatggcag agcacatccg
     2761 ccatgcggaa ggcggaattt ttatccagtc tgaaagcgaa gtggccgcga tcaatatgat
     2821 tgccggagca agcgcggccg ggttaagagt gatgacctcc tcctcgagtc ccgggatttc
     2881 attgaaacag gaaggcattt cgtccatggc cgcctgtgaa ttgccgggtg tgatcgtcaa
     2941 tatcatgcgg ggcggtccgg ggctgggcaa catcagaccc tcccagggag attatttcca
     3001 ggccacgcgc ggcggcggac acggcgatta ccgaaccatc gtattggctc cggcaagcgg
     3061 ccaggaactt tccgatttga ccatggaggg tttcgatctt gccgacaaat accggaatcc
     3121 cgttatgatt ctggccgacg gcatgatggg acaaatgatg gagccggtgc ttttcaaaaa
     3181 acccaaacct cgcaagtaca gcgaaaagtt catgctgcgc ggcgccggca caggcaaaag
     3241 caaattcatt cacggtttga ttctggatcc gatcgacatg gaagagcata actggaaact
     3301 cgcgcgcaaa tatgaagtta ttactcaaaa agaacctcgt tttgaagaac ataagatcga
     3361 agatgcaaaa atggtgatcg tggcttatgg cacggcagcc cgcatcgcaa aaggcgccat
     3421 caagcggctg cgcgcccagg gtgttaaagt cggacttttc cgtccggtca ccttatggcc
     3481 ttttccggaa cttcgtctga gaagtttatc ggaaatcgtg aagcattacc ttgtttttga
     3541 aatgagcacg gggcagatgc tggaggatgt gcgcctgtcc ctgcagggct acgcggatat
     3601 taaatttcac ggccgtccgg gcggtgcggt gccgacaccg gcggaactgg ccaatgtcat
     3661 cgccaagttc tatgagaaaa tcgactaggg atgcaaaggt tggcggatat ggttaaaact
     3721 gtttttaaac gaccgagaag tttgaaggaa aatgtttttc actactgtcc gggatgcggt
     3781 cacagtattg cacacaggat tattgccgaa gtcattgatg aactggatat ccggggcatc
     3841 accatcggtg ttccgccggc cggttgcgcg gttctggcgt acaattactt cgatgtcgat
     3901 atgatcgaag cgctgcatgg acggggtccg gccatggctt cagccatcaa acgctctctg
     3961 cccgaccggg tcgtattttc ctatcagggc gatggcgatc tggcagccat cggcatcgcg
     4021 gaaagctttc acgcggccaa tcgcggcgaa aatatcacca tcatattcat taataatgcc
     4081 gtttacggaa tgacgggcgg acagatggct ccgaccacga tgctggaaca aaaaacgacg
     4141 acatcccctg ccggccggca tagaacgctg gacggctatc ccgtcaaagt cagtgaaatt
     4201 ttttcgcaac tggaaggcgt tacgtatctg gaacgatgca tggtgaattc gcctctcaat
     4261 atcaacaaaa cgaaaaaagc cattaaaaaa gcgtttcaat gtcaactgga cgggtttgga
     4321 ttttccctga tcgaaattct ttccccctgc cccaccaact ggaagatgac agccttcaat
     4381 tcctgtaaat ggattgatga ggtgatgagc aagacgtttc cccccggggt ttataaagac
     4441 aatattgccg cggcaaaaaa atctgcggag gggcgctgat gctgattaaa acgattttct
     4501 caggattcgg cggccagggt gttttgatga tgggggtgag cttcgcccac agcggaatga
     4561 gccagggcta tcaggtcacc taccttccgg cctatggagc cgaaatgcgc ggcggaaccg
     4621 ccaactgcac cgtggccatt gcagatgagg acattgcgtc ccccgtggcg tcggaaccgg
     4681 actttctggt ggccatgaat tccccgtcgc tttttacgtt tcaaaataaa atttccagcg
     4741 gcggaaccat ttttttaaat tcctccatca tcaaagaaaa accgcatcgc agcgatatca
     4801 aggtgtgctc tatgccctgc gccgatatgg cacaggattt gggcaatatc cgggtatcca
     4861 atacaatcat gatgggggct tttatcaaag tatcggcggt gattcctcct gatgtctacc
     4921 tgaaaagtct ggaaacgatc atgggcagca aaaagaaatc actcgccgaa ctgaaccgta
     4981 aagcctttaa tgcaggatat gagtacgtca agaacgattg aggacagata gatgcctgtt
     5041 aaacaaattt cggttatttt aaataatgta cccggctctt ttgccgcctt aacgcatatt
     5101 cttgatctgg aagatatcag caccaaagcc gtctctgcgg ccagcattgc cgatgacagc
     5161 aaggttcgtc tcgtagtcaa cgatcccgat cgcgcggctg ccgtcctgaa aagctataat
     5221 ctgaatttcg aagtgacgcc ggttctggcg gtcgaggttc ccctgcatgc cggcggcatg
     5281 aacgccatac tcaaaccgct ggccaaggcg gaaatcaaca tccactacct ctacacaacc
     5341 atcaatcgtt tcggcgaaga gacgattgtc attctcggcg tcaacaaaat tgacgaagcc
     5401 agagagattt tggaacaaaa ctggattcat cttattgatg aagaaattta ttccattccc
     5461 tgatgacgta tcattgcatt ggtcatgccc gtatcttcgg aaaaagaaaa aatgctccag
     5521 gaccggatga aaagtcttgg ggtcgccgaa acggatcttg aagaaacctt tattcgttct
     5581 tccggtcccg gcggacaaaa agtcaataaa agttcctcct gcgtttatct gcgtcacctt
     5641 ccgacagggc tgtccgtcaa gtgccagaaa gaacgttctc aatcattaaa ccgttttttg
     5701 gccagacggt tactgttgga taaaatagaa atacagcaga aaggcttgat ggccgaagcc
     5761 aggcaaacga tcgaaaaaat ccgacggcag aaaaggaagc gcagcaaacg agccaaagaa
     5821 aaaattctga ccgacaaaca ccaacaggcg gaaaaaaaga ttttacgcgc aaaagtgtct
     5881 gtcgaggaat aacgggcggg aaaacacacg acgggatcgg tcccgccggt tgctgttttg
     5941 cgtcatcacc ctgctgtcca tttctgattt tcgtttaaag aattttggat aaaaaaaggg
     6001 gaagaagtcc tttgttattt gcttcttccc cttttaaata tagcggtgtt aaattacatt
     6061 ccgataatct tcaacagcgg attcgcgaag agaattacca gagaaataac cagagcataa
     6121 atagcgacgg attccgtcat ggccataccg accatcatgg tcaacatgac tttgccctga
     6181 gcttcggggt ttctaccgac ggcattgctt gctccgcttg ttgcctgacc aagacccaaa
     6241 ccggcgccga ttgcacccag tccgatggca agacctgctg ccaatgcaaa tatgctggcc
     6301 acgattgctt tcgtataatc aaccgttccc gcgggagcct gagccgctgc ttccggagcc
     6361 gcaaaaacaa aaggtgcgct aacgattacc ataaccattg ccaacatgac atacattaaa
     6421 ctctttttca tttaactttc tccttttaaa tttaaaatac tgactgcaaa aatccctgct
     6481 atgttttttc acacctcctt tcgcgtttca tctttttttg ggatctttga gtgcttctct
     6541 tttaacggca caaaaagaaa ttgtcaagac aaattttcat taatcgggca acttgatttt
     6601 tttttgcgat acagtataga atccattcat gagaaagtta acgtcgaaac aaaaaaaaat
     6661 cgcacgcctg cttcagaaag acattccggt aacgaaattc ccgtttaaaa aattgtccgg
     6721 cgctccaacg cccggggcaa aagaaattct gaccgctctt gaccagtttc gtgaacaagg
     6781 gctcatccgg aaattcggcg caatcctgcg ccatcagaag gccggatacg gacagaacgc
     6841 tttggtgctt tggtctgtgc cgccctccca aaccgagaca accggaaggc tttttgcatc
     6901 tttctccttc atctcccact gttatgaaag gcatcccgcg tttttggaaa gatacaatct
     6961 ctttacaatg ctgcattcgg acgggcggag catttcatca ttgattaagg acatggtaaa
     7021 tgctacaggc atcggggatt atttaatcct caaaagcctc aaagaataca aaaaaacatc
     7081 tccggagtat ttctgatgaa ggaaacaaca tccgttcaat tttccaaggc gaaccgggtg
     7141 attgccggcg gggtcaattc tccggtacgc gcctggaaat cggttggcgg tcatccccgc
     7201 tttgtgctga aagcaaaagg cagcaggatt tacgatacct tgggaaaagc ctatatcgat
     7261 tacgtgctct catggggacc gatgattctc ggccacgccc atccggacgt aacggcagcc
     7321 gttcgggcag ccttgaaaca agggaccagt ttcggcgccc cgacactgtc tgagaccgcc
     7381 attgctgaaa tgatttcctc agctcttccg tcgatcgaac ggatacgcat gacatcctcc
     7441 ggcacggaag cggcgatgac ggccataagg cttgcccggg gatataccgg acggaagctc
     7501 atagcaaaat ttgacggttg ttatcacggg cacagcgatt ccgtgctcat taaagccggt
     7561 tcgggaccga cgacactggg cattcccggt tccccgggag ttcctgatga tatcgcccgg
     7621 ctcacgctga gtcttcccta caatgatact aacgcccttg caaaaaccgt aaagcggtat
     7681 ggatcgcagc ttgctgccgt tattgttgag ccggtcgccg gcaacatggg cgtcgttgcg
     7741 ccgcgagaag aatttctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaaa
     7861 ggaattcctg tctgcatcaa ccgtgttttt tcgatgttca ccgttttttt tcaggaaggc
     7921 cctgtcgttg atctgcacac ggcattgcaa tccgacacaa acatgtatgc ccggtttttt
     7981 cacggcatgt tgaaaaacgg catctggctc gcgccttctc aatacgaggc gggctttctg
     8041 tcttttgccc acaccgaaaa agatcttcag aaaacccttg aggcctgcgc aaaaacattg
     8101 agaaatcttt aatcgtttca ttgacaagca ttcttcaatc cggtatgaaa cgacataaat
     8161 aatttcaaag attcaggagt ccagatgaaa aaagctttcc tgaccggcat taccggacaa
     8221 gacggttctt atttagcgga atttttactg cacaagggat acgaagtgca cggattgatc
     8281 cgacggtcga gcacattcaa taccgaacgc atcgatcacc tctaccatga ttttcatgat
     8341 cccgaagccc gattgtttct tcactacggg gatttgagcg tctccgcgca attgatggat
     8401 ttgctccatt ccgtcaaacc cgatgaaatt tacaatctgg gcgcacagag tcatgtgcgc
     8461 gtgagcttcg acatgcccga gtacaccggc gacgtcaccg cgctgggaac tctgagaatt
     8521 cttgaagcaa tccgcaaaac gggcattacg acaaaattct accaggcgtc atcaagcgaa
     8581 atgttcgggg ccgcaccgcc gccgcagcat gaaaaaaccg ttttccagcc ccgcagtccg
     8641 tatgcggccg ccaaggttta ttcctattac atcacgcaga attatcggga tgcctacggc
     8701 atttttgcgg ccaacggaat tcttttcaat catgaatctc cccggcgcgg cgaaacattc
     8761 gtgaccagaa aaatcacccg tgcggcaacc cagatcaaac tcggtctcaa ggacaagttg
     8821 tatctgggaa atctcgaagc caaacgggac tggggatttg ccggggatta tgtggaagcg
     8881 atgtggctga tgctgcagca gaacacgccg gacgactttg tcattgccac cggagagacc
     8941 cattccgtcc gggaattcgc agaaatggtt tttcaaaaac ttgatctgga ttacaaacgt
     9001 tatgtggaaa tcgataaacg gtacttccgt cctacagagg tcgacgctct gctgggcgat
     9061 tcgacaaaag caaaaaaaat attgaactgg caaccccggg tgtccttcga gaaccttgtc
     9121 gatatgatga tcgcatcaga catggagctt gcccaaaaag aaaaaacgct cctggatgcg
     9181 ggtttcgttt ccaataatca aaatttcaac cattaaggct ttgccaatgc tacctcaaaa
     9241 acggattacc atcaccggcg ccaggggatt tctgggaacg catctgctga aaaaattcaa
     9301 aagcaaaggc tgccggaata tcgaaatagc cgaccttccg gaatataacc tgacggacat
     9361 taacgcgatc cggagaatgt atgaggaaac gaagccggat gtcgtcattc atcttgctgc
     9421 caaagtggga ggcatcgggt tcaatcagga aaaaccggcg gaactttttt atgataattt
     9481 aatcatggga gcgcagttgc tccacgaagc acacataaga aaaattgaaa aatttgtcgc
     9541 cctgggaacc gtttgcgcct accccaagtt taccccgacg cctttccgcg aggaagacat
     9601 ctggaacggc tatccggaag aaaccaacgc cccctatgga cttgccaaaa aaatgatgct
     9661 ggtgcaatcg caggcttacc ggcaacaata cgggtttaac tcggtatttt tgcttccggt
     9721 caatctgtat gggccgggag ataattttga ttcccgttcg tcgcatgtca ttcccgcatt
     9781 aatcaaaaaa tgctttgacg ccatcgatcg tcaatccgat cagctggagg tgtggggcac
     9841 gggctcggcc acccgggaat tcttctatgt ggaggatgcc gcagaagcta tttatctggc
     9901 gacgttactg tacgacaaaa gcgatcccgt caatctcgga tcggggtttg aaatttccat
     9961 aaaagatctg acaactctga ttgccgaatt gaccggattt tccggccgga ttgtgtggga
    10021 ttcctccaag cccgatggac agcctaagcg tatgctggac acatccaaag cattgaaaga
    10081 attcggattt attgccaaaa cagatattcg gacgggactc accaagacga ttgattggta
    10141 taagaaaaat cgtcatctgt taaaaaaatg aacgccggtc atttttttat tgactttaat
    10201 ggctgcaggt gctaaggagc caatcagcga atggataaag aaacgcgaaa gtatgcgatg
    10261 caaatggcat ttgccagcag catcggcatt gccatgattc tggcagtttt cggttgtctt
    10321 ctgctggggt cgtatttgga caaaaaattc gattccggtc atgtttttac ggttgttttt
    10381 ttattcatag ggttagcggc cggatttcgt aatttatatg ttttagttca aaagaatttt
    10441 acggatgaag aaaccattat aaagagttta aaaagtgaac ctcatcgtca aagacccgct
    10501 ccaaaaaagg attgaaatca ccaactggat cattctggcc gttattttca tcccatccct
    10561 gtttttagct ccgttaaaat tttctctcgg catattgctc ggcggttttg tcagtattgt
    10621 caattttcat tggatggccc gggggttacg cggtattttt caaaacctgc aagggaatcc
    10681 caaaggcact gtgatggtaa aatattacat ccgcctggcc atcatggccg ttgtgctgta
    10741 tcttttaatc gccaataatg tcgttgacat cattggactg cttatcggac tttccgttgt
    10801 gataatcaat attctggcaa cgatgattat cgccctggca aaaaaaaact ttattgagga
    10861 ggttgtataa aaatagatgg aacccttaat attttttcac acgcaatggc tgaaagatgt
    10921 gggtattgac atgacgattg tctttaacac attgtttgta atggctcttt tagccttttt
    10981 ttcctttttg gccaccagac gtacagaagt ttatcccgga aaaactcaaa acgtcatgga
    11041 agtcattatc ggcggattcg atggtctgct gaatgaaatc atgggacaca atggaagaaa
    11101 gttttttgct ttgatctcca ctctggggct ctttattctt gtgtcgaacc tcatcggaat
    11161 cgttcccgga tttgaatctc cgacatccaa tatcaacacc aatgcggcga tggctttgat
    11221 tgtctttctt tccacacatg ttgtgggcat caaggaacac ggcttaaaat atttcaaaca
    11281 gttcatgggg ccggtatggt ggctgacccc cttaatgctg cccatcgaaa tcatcagcca
    11341 catttcgcga ccgctttccc ttactttccg tcttttcgga aatatcaagg gtgaagacat
    11401 cgtccttctg gtcgttctct ttctggttcc ctggttcgtt cctcttcccg tgtttgtgtt
    11461 aatggttttt acatccgttg tccagacgct cgttttcatg cttctggcca tgatgtatat
    11521 tgccggggct atggaagaag gccattaaaa gaaattagaa atcattgatc accggtggcg
    11581 tgaaatcatt atttaaaatg atttcccgcc gtctttttct acacgctttc acgtccggga
    11641 tcggaaaatc ccatattatt gatattaaaa aagattgttt tttttagact cttttttgca
    11701 tctaaaactt gacgatttga ttgtggcgta atatttaaaa aaaataaact aaatacacct
    11761 gaaaaatgat tgatgagaga tcatgcagac tttgggaatt aacataggat cgtccagtat
    11821 caagctcatc ttgcttgagg atgatcggat tgtctggcaa aaagtcgagc cccatgaagg
    11881 aaactttgtt cacgctctgg aaaaaacgtt gtccgagcga agcttcccgc ccggcgtatt
    11941 aaccctggcc acgggcacgg aagggagaaa tcttttaaat atcaacagcg tcatcgagcc
    12001 gctttgcatt gaagaagcgc tccggcattt aaacggcaac gttaacgcgc tggtttctct
    12061 gggcggcgaa gatcttgtcg tttacagcat tgatgaaaat cagcaaatca tcacaagctt
    12121 ttccggaaac aaatgcgctt cgggcacggg cgagtttttc aaacagcagc tcggccggat
    12181 gaacatgcat ctcaacgata ttcattccat tcctgaagaa agccggattc tgaaactgtc
    12241 ctcccgctgt tcggtcttca tgaaaagcga ctgtacccac cgtctgaaca agggggaggc
    12301 cagcacgggc gacatcgtgc tttcgttgag cgatgtcatg gcaaccaagg tcatcgattt
    12361 cattaaaaaa gcaaaaataa ccggcaaaaa agtcctgttg gtcgggggcg tcaccaggaa
    12421 cccgcatctg atccgcttca tccgccaacg catgccggaa aatgatttta tcgtccccga
    12481 tcaggctgtt tactttgaag cctacggcgc cgccctgctg gccggctcga tgggcactcc
    12541 cctgcccgcc ttgaacgatc ttttcaaaac ccatatggtc cagttcaaga gattcaaaag
    12601 cttgaaaacg gccgagggga aagttcatta tcttccatcc aaaaaatcaa aggtcattgc
    12661 ggggagggaa tatattctcg gggtcgacgg agggtcaacc accaccaaag cctgtctcat
    12721 tgatatggaa acctccgaga ttacggcctc cttttacgga cgcacgcatg gagatccggt
    12781 caaagccctg aaaaattgct tcttggagat gcaaaagcag atccgggagg atatcggcga
    12841 ccgagcaatt aaaataaccc tggccgccac caccggatcc tccagagaac tgctgggcgt
    12901 ttttctggaa acgcccggcg tgtataatga aatcattgct catgcggtgg gaacgacgtt
    12961 ttatcaggaa gacatcgaca ccatctttga aatcggcgga caggatgcca agtacgttta
    13021 tctgaaaaac aaggtgccga ttgactatgc aatgaatgaa gcctgttccg ccggaaccgg
    13081 ttcctttctt gaagaatccg cgcagggcga tctgaacatt catcatgcct gggaaatcgg
    13141 cgatatcgcg cttggcgcgt cggaacccct gaagttcggg gagcactgtt ccgctttcat
    13201 caattcggat gtccggaaag ccattcagca gggcgcgtcg cgtgaagaca ttacggcggg
    13261 tatcgttacc tccatcgtat ccaattatct caaccgcgtt gtgggcaaca gaaccgtcgg
    13321 ccaaaacatc gtcctgcagg gaggcgtcgc caagaacaag gcggttcctc tggctttcgc
    13381 catgcttctg gacaagaata ttctggtgcc tcccgatccg gaactgatgg gttgcttcgg
    13441 cgtcggcatt cttgcccgac agaaactgca tgagggattt ctcagcagaa gagattacga
    13501 tattgaaaag attctgtcga ccgagattct ccatgaaaag gaatttcagt gcagcgcctg
    13561 cgataacttc tgcccgatac gcgtactgaa tgtcaacggc cgcaaatata tgttcggcgg
    13621 ccgttgtaac aaatacgcca acatcaggaa gaaaaaaagt ttcgatgaaa gcgatgttgt
    13681 cgattatatc gaacagcgca acgacctact ctttaatcaa tgcgctcccc gaccggccga
    13741 cctgatcaga aaaaaggatt tcgtcgtcgg cattccgaaa tgcttttccg tctattcgca
    13801 ctggccgctt tacgcttggt ttttccatac gctcggtgtg gaaaccgtcc tgtccagcga
    13861 catcacacac gagggcgttg cccgggtgga aagcacgtac tgttttccgg cggaaattgc
    13921 ccacggtgcc gttcaggata ttttcaaccg caacgtggat tatatttttc tgccgcattt
    13981 ccgcgatatg gaaagctatg aaagcaatac accggcctgt ttctgtccga tcacgcagag
    14041 tttgccttac tatattaaaa aagcctttcc agaaattccg gaggggaaat acctcgctcc
    14101 cgttgtcagt tttatgtacg ggacacagaa agcctccgaa tcgtttatcg ccatggctgt
    14161 ccggctcggt tttgatcagg aggacgcccg cctggctttt tccgttgccc tggaaaagca
    14221 aatggaatgc tttaggaaat tcgccgaact gggtaaaata gcaattgaaa attcccgcaa
    14281 atcccaaaga cccgttattg ccgttctggg gcggccctac aacgcgttta cacccgatgc
    14341 gaacatgggt attccgcgaa aatacacctc ccgcggcttc accgtcgttc cttttgacat
    14401 cctgccattt gaggatcagg ggatttatcc caatatgtac tggtactacg gtcagcagga
    14461 catgaaagct gccgcgcttt tgaaaaacga agacaatatc tttgtaacat acatctccaa
    14521 tttttcctgc gctcccgatt ccttcatgct tcactatctg aagtggataa tggggatgaa
    14581 accctttctg gttctggagc tggattccca cacggccgat gccggcgtgg atacgcgtat
    14641 cgaggccttt ctggacatca tcgaaggtta ccgttccaaa atcagcgaca tcagagaaag
    14701 ccggtatgat aacggtctaa ggtttatcaa cacttccggc gaagacattc atatccgaaa
    14761 caccctcacc ggcgaaaaga ttcccatcaa aaacaatccc cgcgtcaagc tgcttttatc
    14821 caacatggga agactgtcgg tggaagtgct ggccgccacg atccgcgggc tgggtattca
    14881 tgcggaaacc atgcccgtgc cggatatcta tacgctgcaa atggcacgca atcacgcctc
    14941 cggcaaggaa tgcctgccct ctcatctcgt tctgggcagt acgttgaaat atctatcgtc
    15001 ggaaaaatac cgcaaagacg aaatttacat tctgttcgtg ccaaccacaa ccggtccctg
    15061 ccgaaccggc cagtattttg ttttctatga aaatcttttc aaagatctgc gtctggaaaa
    15121 tgtcgtggtt ttcacgctgg acgccgacaa ttcttataac gagctgggaa cggaattttc
    15181 caaatatgcc tggtgggccg tactcatcgg cgattatatg aaggatattg aaacttcgtt
    15241 gcgcacctgt gcggcggatc cggtcaaggc cgtttccgat tacaatatca tctggcaaaa
    15301 actgatcgac gttgcggaaa aagatgtcac gcaatcgctt cccgttctga aggagatatc
    15361 ctcttcgatc gccaaaatcc ccttaaagaa aaaaatcgat gactgcccga aagtattgat
    15421 tgtcggagag atttacgtca ggcgcgatga ttttgcagta gatgaactga tcaggcattt
    15481 cagccggcgc ggtattatcg gcaaggtctc caatgttgcc gagtgggttt actattgtga
    15541 tttcgtccgc cgtcacgaac tgcaaaaacg tctcaaactc gttccctggt acaaaaagtt
    15601 tctggctgcg gaattttccg atatcgtcaa atggaaaatc gagcacgtct ataaaaagaa
    15661 agtggagaaa aacatccgcg acattctttc gacaaccggc ctggttccgc acgctccgca
    15721 caacatggat gaaattatgg aaaacactga aaagcatttc gtcaacaacg aactgtattc
    15781 cgaaatcagc atctccagcg gtgtcgcagc caccgccatg atggacggtt atgccggcat
    15841 cgtcaacatc tccccgtttg cctgcctgat cggccgggtg atcgagggcg tcctgacccc
    15901 ctgggccagg gaaagaaaat atccggccat ctccattgaa atcgacggga atctttttcc
    15961 gcccaatgtg atcagcaaac tggagatatt catgctgaac gtgatgcgat tccggaatca
    16021 ggacgagacg gaccatcgga caacccttta ggaattgatc atatgcagga aaacgtcaca
    16081 ggacttattt tggccggcgg gaaaaattca cgcatgggcg tcaacaaggc gtttctggaa
    16141 atagacggga agcgcctgat ggacaggacc ctgtccgttt atcgcagaat tttcccaaag
    16201 atcattatcg taaccaatga tccgctggcg tacgtggaat tttccgaggc gatgattgtc
    16261 acggacatct acaaaggcaa gggatccctc ggcggaattt atacgggatt attttacgcc
    16321 gcaagcgatg actgttttgt tgccgcctgc gacatgccct gcctgaatga agatttcatc
    16381 cgttacatga tcggacgccg gcgatcatac gacatcatcg tccccgagtt atccgaagga
    16441 ttccaggccc tgcatgccat ctattcgcgc cgctgtctgg ccgttatgaa aaagatgatc
    16501 cttgccgacc gattgaaaat cgccggatta ttcaaagaca tgcatacgct cagactcacc
    16561 gaagaagaca tccggccctt tgccggaaat acacccctct tcacgaacat caacacccct
    16621 caagatttgc agtcttttcg gaaataatcc ggccggatca gtcggatagc ggcgtcggct
    16681 gaacccattt ttgggtttct gcgctgtaaa accatttatc gggaaacccc cgccgatgga
    16741 caaagacagg atgatccgcc cacgccagca actctttgta ggcgctgtga atcttcctga
    16801 aatctgccgc tttgccgccc agatcgggat gatggatttt cacctgacgg cgataggcgc
    16861 ttttgatgac cctgtcgagg tcacaggatg cgagatcgat tttttttatg tccagacaat
    16921 tcagcgctga caccttcacc gccggtattt taactatttt cggccgctgc gatttgtccg
    16981 taatcgtata acgcttcgcc atttttaaca catgctgcga cgcaagataa tttctgtgcg
    17041 ttgtgtccga cacagcccac caggcctttc ccagattgtt ggtcataaaa ctgaaatcga
    17101 tggcaggcgt gtttcccgct ctgcgcctga tggcataccg gaatatctcc ctggacccgt
    17161 agggcacgac atccagaatg atcatggtct tcgaaaaata gaaggttgcg tatttcgtat
    17221 tcaaggcctg cagcaatccg ttcttcaggt tgagtttctg ccgcagattt tgtcggcatt
    17281 tcaccgaaca ataccgtcgt ttgctgaccg ggtctgtttt tccgcaggac aaacaagtat
    17341 gatgatggtt aatttccgaa gcgtgtgtca acatttcatt tctgcaaggc atccggccgt
    17401 tgaaaatacc ggatgccttt tacgacgtta ttttttagcc gctgccagat accccttggc
    17461 cagagccttg ttcacatgca aaacaccttt attcatttct ttcatcggat aatccggcgt
    17521 aatgaccggc aggacatccg cgtcggcctg cgttttgacc acagatcccg ccggcacata
    17581 acggccgtcg tccaccgtga cgcccattaa cgtcacaccc ggttcaataa cgcaatgctt
    17641 ccccacaaaa gatttaaaaa tcagcgtttt cataccgaca aaggtatcgt ccatgacaac
    17701 cgccggcccg tgaatctgca cttgatgcgc cagggaaaca cgcttcccca cataaacggc
    17761 atattttttg ccgtcaacgt ccctctggtt tttttcaacc ggtttgccgt ccagttctgt
    17821 ttccagagca tgaatgatca cgccgtcctg cacattggaa tcatcgccga catgcagcgg
    17881 ctgtccttcg tcgccgcgta tgcaggctgt cggggcaacc atgatatttt cacccagaat
    17941 gacattgccg ataaccgcgg ccagcggatg gacataggta cccgcaccga tgaccggttc
    18001 ggatattttt gaactgaagt ctgtttttac gtttttttcg atcattgttt cctccctgat
    18061 ttgttctcga ataatgacgg cctgtgttta tcaggccgat ttggtttttt ccagtcggac
    18121 aagttctctc cagacccgat ccaccgtttt ttttgtgcgc gcaatgctgt ccctgttgtc
    18181 gatcacaata tccgccagcg ccgttttttt gccgatcggc atctgacttt ttaatcgtgc
    18241 ccgggcgtcg tctccggata tgcgattacg ggaaatcaaa cggcagatct gatcctccgg
    18301 atcggacaac accaggatgg tgaggtcgaa aagatgtgtt tttttcccct cgaataatag
    18361 cggcacatcc gacaaaacaa tggcatgttt ttctttcctg ccgatttttt ccaggcgcac
    18421 ctgccattcc ctgtaaaccg gtggatgaac gagcttgttt aaaacccgga gttttttttt
    18481 gtcggcaaag acaatgcggc ccagtttgac gcggtctatt ttttgatccg gacggagaat
    18541 ttgcctgccg aaataatcga cgactttctg ccacgcgggc tgtcccggtt cctgcagccg
    18601 atgggccagt ttgtcgaagt ctatgagatg cgctcccttt tcaacaaaca ttttcgcgac
    18661 ggttgatttt ccgcaggcga taccgcctgt cagtccgaca ttgagcataa ttatttttta
    18721 ttcagttcgt agataaaacg caacccgtcg agggtaagca tctcttctat cttttcaatg
    18781 gttttggtct cgggtgcgat aatattggcc aggcctcccg tggcgatgac cagcggtttt
    18841 gtctcgactt ccgtcctgat cctgttgacg attccgtcga caagtccggc gtaaccgaac
    18901 ataattcccg cctgaatagc gctgaccgta tctttggtaa tcgcggattt gggccggctg
    18961 aattccacac gcggcagttt tgacgctttt tcaaaaagcg cttcgctgga aatgacaatc
    19021 cccggagcaa tgcaccctcc catatattct cctttcgggg aaacataatc aaacgttgtg
    19081 gccgtgccga aatcgacgat aatggattcc ttccggtatt tattgtacgc ggccacggca
    19141 ttgacgattc tgtcggcacc gacttctttg ggattatcgt agaagatcgg catcccggtt
    19201 ttaatgcctg gtccgacgat cagcggcttg acattgaaat acttgataca gagcggttcc
    19261 agaatattga gcatgggagg aaccacgcat gagataataa tggcggaaac cgttttgaag
    19321 ggtttttctt ttttccggct ggactgatac aggttataaa tcagcacacc atattcatca
    19381 atggtgtggt cgatttccgt ccgtatgcgc cagtcatgga tcagattttt tccgtcaaaa
    19441 agccccagaa cggtgttggt atttccgaca tcaatcacca aaagcattat ctttccacct
    19501 cataatcgtt gcgtctcctg cagagacttt aatttcttga ttgccctcaa cgacaagaat
    19561 caggcaaccg tcatcaccca gccctttggc tatgccttct gtgctttcac ccttaaacgc
    19621 gatccggatg ggccgtccta tcatcgccgc cgattcgagc cacttttttc ggatgggctc
    19681 aaatcctttt tgcagtagct gtttatacca ttttccaaga ttttcataga ggctaattaa
    19741 caaatcgtga cgggaaattt ctccgcccga ctcgatcaac agagacgttg ccgactcccg
    19801 aatggtcttt ggaaaatggt gcaaacccgc gttgacatta ataccgatac cgacaacggc
    19861 aaaatcaatt ttttttgatg tgatttttgc ctgggacagg atgccgcaga ctttttttgg
    19921 gccgattatg acatcattgg gccacttgag ctttacccgg ccgggacaaa cgccgtccag
    19981 aacttctgct gcggctacgc ctgccatgat aggaatctga gacgccccgg aagattcgca
    20041 ttccggcctt aaaacgatgg ttgtataaat attcaccccg ggcggtgaaa tccaggagcg
    20101 ctgccagcgc cctcttccgg cagtctgcgc atccgccaga acggcagtgc cttccggtgc
    20161 acccgccatc gccagtgcga cggcttcgtc gtttgtcgat acgatttcag gataaaaatg
    20221 aagatggccg ccgaatagtc ctgtgccgag ggtttcctgc aatcgggcga tgttccaatc
    20281 catacatcaa gagcattcca tcaggatgaa gacctgattt tgagtgaaat gtcggccgcc
    20341 tgcacggaat gggtcagttc cccgaccgag ataaaatcta cgccggttgc tgcaatatcc
    20401 gtcacattct tcagaccgac attgcccgat gcttccagaa gcgcacgccc ggctacaaac
    20461 gatacggctc ttttcatggc cgaaacgctc atattatcca gcataatgat atcggcgccg
    20521 cactcaaggg cctcccggac ttcatccaga ttccgggttt ctacttcgat tttcatgaaa
    20581 cgtgaaagtt tttttctttg cgcctttacc gccgctgtga ttccgcctgc cgcctcgatg
    20641 tgattatctt tgatgagcac accgtcgtaa aggccgaacc ggtgattttg tcctccgccg
    20701 gttttgaccg ccattttatc caaaacgcgc aatccgggaa cggtttttcg tgtatcgaga
    20761 attctggctt tcgttttacg aaccgccctg acataccggt gggtcagcgt ggcaatgccg
    20821 cacatccgct gaaacagatt cagagcaacg cgttccgcca ttagaatatg gtccaaccgg
    20881 cctgatactt cggcgatgat gtcgcctttc tttaccggat atccttccgg aatccttttt
    20941 tggacattga tgtttttatc cagcgtcctg aatgtcccgg caaatacgtc cgttccggca
    21001 acaataaaat tccccttggc aatggccacc gccctgccct tttttcgggc attgaccgtc
    21061 gcgcctgtcg taatatcgcc ggatccgata tcctcggcca atgcattttg aatgattctc
    21121 acaatcctct gttccatcag gcggacatgc ctttcaattt cttaagagcg ttttccagag
    21181 tggtgtgttt tttctggaaa tccttcacct tgtcttcctc tttctgaatg atgtccggcg
    21241 cggcttttga cataaaatcg cgattggcca gctttttcga actctgctgc agatcttttg
    21301 atatcttggc cagttccttt tccagacggg ctatttcgcc ggaaagatca acgatgccga
    21361 caatcggaac gaatagtttt gtcgagccga caaccgccgt ggccacacct tttggttcca
    21421 ccgcattgat ttcaacggtt aaggattcca gattggccag atttaaaata tagcctttat
    21481 gtttctccac catttttttg gttttttcat cgacggcgga cattaaaaca tcgagctttt
    21541 gcgacggcgc aatgcgcatt tcaccccgga tattccggac ggcggtgatc atnnnnnnnn
    21601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnttaaagcg gtattcatcc agggcggtcg
    21721 tcacgtcctc caccgcttta ttcagacggg ccttgatcca caaatccggc agcgaatcga
    21781 caacctccgc agttgcggtt tcgtcaaaac cgtccagatt ggcaaatgtc aatttggagg
    21841 cgttccagat tttattaaca aagaatttat agccctcgat gcgttcgggg gaaaggcgca
    21901 catcgcgtcc ctgagcggta taggcggcca gggtgaaacg aaacgcgtcc gttccgtatt
    21961 gatcaatcat taagagcgga tcaatgatgt tgcctttgga tttgctcatc ttctcgcctt
    22021 tttcatccct gaccagcgca tgcaggtaca catgcttgaa gggaacgtcc ttcatcacgt
    22081 aaaggcccat catcatcatg cgggcaaccc agaaaaagag aatgtcgaat ccggtaatta
    22141 acaacgaggt cggataaaac gttttcagag cagccgtttt ttccggccag cccagcgtgg
    22201 aaaaaggcca gagcgcggaa ctgaaccagg tatccaggac atcttcgtcc tgcttcagag
    22261 aggcgccgcc gcaatcggga caggtgtcgg gatccaccgt ggacacaatc acttttttac
    22321 agttgtcgca ataccagacc ggaatgcggt gcccccacca gagctggcgg gaaatgcacc
    22381 agtcgcggat gttgttcatc cagtcaaaat acgttgcttc ccatgttttg ggtacgattt
    22441 tcgttttccc cttgatcacc gcggcggtgg cctgtttggc cagaggcttg atttttacaa
    22501 accactgttt ggagaccatc ggttcgatgt cggttttaca gcggtagcat tgtccgacat
    22561 tatgagcata cggctcggta ccgaccagat atccgccctt ttgaagatcg ttgacaatgt
    22621 ttttgcgggc ttcataacga tcctgacctt tataggctcc gccgttttca ttgatgatcc
    22681 ccttgccgtc aatgacggta atgatctcca gatgatgtcg ctgggccatg gcaaaatcgt
    22741 tgggatccga tgccggcgtg atttttacag cccccgatcc gaaatccatc gtcacataat
    22801 cgtcggcgat aaccggtatt ttcttattca tcaagggcag tatgacgtat ttgccgatca
    22861 ggtttttgta tcgtttgtcg tccgggtgaa cagccactgc ggtatcgccg agcatggttt
    22921 ccggccgggt cgttgccacc acaatttcgc cttgcccctc ggcgaaggga taacggatgt
    22981 tccacaggaa gcttgcatca gatttgaatt ccacttccag atcggaaatg gcggtgtggc
    23041 agcgcggaca ccagttcacg atataatcgc cctgataaat catgccttca ttgtagagac
    23101 gaacgaatac ctcgcgaacg gcatgggaca atccttcatc catcgtaaag cgctcccgtt
    23161 cccagtcgca ggagcatccc aggcgtttta actgattgat aattaccccg ccatatttcc
    23221 ggcgccattc ccatacccgg tcgataaatt tttcacgtcc caggtcgtga cgcgatgttc
    23281 cttccttgag caactgctgt tccaccacat tctgggtggc aatgcccgca tgatccgtac
    23341 cgggcatcca cagcgtgttg aatccctgca ttctcttgaa acggataagg atatcctgca
    23401 aggtattatt caacgcatgc cccatgtgca gcatacccgt cacgttcggc ggcggaatga
    23461 cgatggagaa cgcctcggcc tcgcttttat cctgagcgtg aaaatattta tttttcaacc
    23521 agtattcata taatcttttt tcggctgcgg ccggttcaaa agccttgccc aattctcttt
    23581 ttgccatgcg atcattactc ctcattcggt tctacaaacg aaaagggctt ctcgcccctt
    23641 tattatttat attttatatt ttatatttta atgacttatt aatcgatctt taaattctcc
    23701 ttaagctttt taaccgccag aagcgcttca acaccggaca tcggttttaa gggggaaaac
    23761 tcgcccgttt ttgtattttt tgcctccatg attccccgtg atgtcactga aataatcggc
    23821 gtaaaataag gcatgtctgc gggaacatcc ggaaataacg aattggaact gacgtatctg
    23881 gcggcgatat atctctctcc cgatactttt atcagaatat cttcgagaat aattgcgaat
    23941 tcgccgcggg agagaatttc atccggttta aaattgcctt tgggatcatt ttcgagaccg
    24001 tgaacttttg cttcgaggac ggcttcaatg caatttttca aaggatgaag ggcaatgtca
    24061 ttggccagca gtttcaccgg ttctttctcc tgcaaaacca tgtaggaatg aaactgatcc
    24121 attttttcca tttcttttgc aacgtgttca gcctggctca tcgacgcggc aactttttcc
    24181 tcttcggttt gcgcctcctg tgccgttttc ggcaaatatt tttgatagag ctcttttatt
    24241 tttagctcct cggccagcat catggcgata tctgcccggc tcaccgtttc cttgagcgcg
    24301 atttgcttcc cggcttgcgt ggcggggtgg gctttgtgca ccagctccag gaactccaac
    24361 cggttattgg catcctcgat atgaccggct ttcagctgaa tgactttgtt gaacattttt
    24421 gccgcctggt cgaattccaa cgcttcctga taggcaatgc ccatgaaata gtaaacaggc
    24481 gcgtattgag aatcgatcat cacggtctcg acaaaataat actttgcctt ctccagccat
    24541 ttttcatcgg atttttttgc cgtgtgataa cgaatagccg ccgtgttgac aaaaagnnnn
    24601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgaa atcgatttta taccgcatgg
    24721 tgcttaagac atccatgccg agcagaccgt cgtatgacgt atcaccgtcc ttattttcca
    24781 caacaaaaat attccagtcg cgaaaggtat tggggccgat cctgagataa ctgaacgtca
    24841 cacgattggc atcaatggtt ccaccgccga caacccgccc ctgcgccttt ggcgcattat
    24901 ttaaatttac cagcaatcgg ccggctacat tgtcgtagat ggtcgtggcg gaagcaccgg
    24961 tatccagaac caaacggatg tctgcctccc ggccgtcata cccgagcgtt acaggaacca
    25021 aaataccgtt ttctttgacg gcgattctgg ttgttttttt ctcgggcgat tccgcattgg
    25081 gcgcaatggg tctatccgtc tttggaatct ccgtctcctc ctgcgatacc atttcggtgc
    25141 aaacctgatc ctccagaggg taatcggaaa acgtctgaag gccgcttcgg tccgcacagt
    25201 ggtaatgagt tgccgcgcca atattcaacg gcagggcaat caggacgacg acaaaaagcc
    25261 tctgaagccg gcacaaatac tcaacggacg tatggtgatc tcttcgggtc attcagtctc
    25321 ctgattttca ttttgaaata ttttaacatg tcttctcaga atgtccagtg catcgggcgc
    25381 aaaccagttg atttccttat cggcggaaaa ccaggtcatc tgacgccggg cgtattgcca
    25441 cgtgtcgcgt ttaatgaaat tcacacagtg gcgccagtca tattgccagt tgaggaatcc
    25501 gatgatctgt ttatagccca gcgattgcag cgatttcaga ttttctctgt atcctcttgc
    25561 cagaacgcct ttgacctcat ccagcaggcc ggcccggatc attgcatctg tccgcttttc
    25621 aatcctttcc ttcaattcgc cgcgttccac cctgagtcca atcttgcagg ttgtgtaacg
    25681 gtcttcgctg aaagaatgtt ttttttgttt cactgtaatc gattcgccgg tctgttccag
    25741 cacctccagg gcccggatca cgcggaccga atcgttcgga ttgatttgcg cggcagccgt
    25801 ggaatctttt tgccggagca aatcataaag atagattttg ccgtgcgttt cccggaggct
    25861 ccgataatga tttctgatgg atgcatcgac atccggggtg tcgataattc ccttcagcaa
    25921 tgcccggata taaagccccg ttcctccgac aacgaagacc ggcttcccct gatgaaccag
    25981 ttcctcaatg accgcccggg cgcgtgcggc aaataaagcg gcgttgaatt ccccgtcggg
    26041 attcacgaca tcaatcagat gatgcctgat cccacggcgc tgttccatcg tcggcttggc
    26101 cgtaccgata tcgagataac ggtagacctg catggaatcc gcgctgataa tctcaccgcc
    26161 gcattccaaa gccagataca cggccagatc cgttttcccc gtggcggtcg gtgagttgat
    26221 gataaccagc ggcagtttct tcatcttcgg tttttttact tccttttgaa cagtctttcg
    26281 atttcgctta aggagaactg aatggtgatc ggacgtccgt gcggacaggt tgcatgaaac
    26341 ggcgtttccg caagttcgcg gcacagcgcg tccacttcgt cccgggaaag caatcggttg
    26401 gctttgatcg ccgctttaca ggccagggag gccagaaggt tgtcgctgcg ttcctgcaga
    26461 gaaggcgttc tgttctggtc ggcaagctga tcaacgatat cgtatatcat ttcgctgatc
    26521 ttcactttgg ataacattgt cggcaccgtc tttacggcaa ccgcgtcccg gccgaagcat
    26581 tcgatttcca gaccgatcgt ttcgagaaaa tcaagagcat cgttgagcaa ggccatttgt
    26641 cccggtgtca ggttgacaat ttccggcagc agcagcgact gaccggccgc ctttttaccg
    26701 gagtctttct ttagtttctc cagaataatg cgttcatgcg ctgcatgctg atcaagcaga
    26761 atgaggctat ccggtccctc aaaaagaaga taggtgccgt cgtactgacc cacatatctc
    26821 atgtcgccaa ataaaattgt tttatcaggg acacccgcat ccccttctga agcagccagc
    26881 ggttgcgaca cctgtgagcg gtgcaataaa atgttttgaa ttccctgttg tgcatgcgac
    26941 cggtcagcgg gtctggaagg catggacgga gacggctcat tcacaaaagg ctgccgatgg
    27001 aaataatctt ttttttccgc aggcgccaga cgatacaggt aatgtcctct ggatgtttcg
    27061 gcacgggaca aatgatgagc aatggttttc gagaccagtt cataaacgcc atgcgcattt
    27121 ttaaagcgca cctccatctt cgcaggatgg acgttgacat ccacgtcttc gggcggcacc
    27181 tccagaaaca gcgccgcggc gggataacgt ctggactcaa taatctgccg gtatgccgac
    27241 aatacggcat gcgtcaggct ggtatcccgt atgaatcttt tattcacaaa cagataaatg
    27301 cttttggaat tcgatttgga gtattccgga cgcgaaataa atcccttcag ggtcatggcg
    27361 tcctgctgtg catcaactgc catgcaatgc gccgaaaact cgccgcccat gaccatggca
    27421 atgcgcttgg ccgtgtcatg gacttccgga gccgcgaata tttccttccc gtttgcctcg
    27481 actttgaaac ggatttccgg atgggccagg gccagacggg taatcacatc cagacaggcc
    27541 ccctgttccg tcgcttccgt ttttaaaaat ttacggcggg cgggaacatg ggcaaagatg
    27601 ccgctgaccg atatctgcgt gccttcgggc gttccggccg gatccatggc ccggatcaca
    27661 ccgccgtcca gaatcgcccg ggtgccggac acatcatttt tccgtctggt taacatttct
    27721 actctcgcga cggaggcaat gctcggcatc gcttcgccgc gaaaaccgaa cgacacaaca
    27781 ttgaatatgt cttcaaattg atgaatcttg ctggtggcat gtctttcaaa aaccagcggg
    27841 acatcgtcgt gatcgatgcc ggagccgttg tcggtgaccc tgatggactg cgtaccgccc
    27901 ttttccaata caacccggat atcggtcgcc ccggcgtcaa tggcgttttc cagcaattcc
    27961 ttgacgatgg aagccggccg ctctacgact tctccggcgg caatcttatt ggcgatgtct
    28021 tcggacagaa caatgatgcg cttggacaat atttatttct cctgataaac tcggttatac
    28081 taatattaca taaaaacaaa atccgcaacc atgattaccg gacagtcatc ggcggtttga
    28141 acatttttct ttcttgacaa agcgtgatgg atttgaatag aaaaaaaagc ttaatcttgc
    28201 tgcaggtgaa ttgcatatgg aaaaattttt cgatccccat tctgttgttg ttgtcggcgc
    28261 ctccaatacc ccttttaacc ttggagctac aatttgtaac atgctgaagg attatcttca
    28321 atatcccggt gcagtttacg cggtcaattc gaaaggcgaa aaggtgaacg gctatccggg
    28381 ttattcatcg atactcgatc ttcccgaaag tccggatctg gccgtcatta tcgtggcggc
    28441 caaaaacgtc cccaggatca tcggcgattg cgcccggaaa ggaatcaagc gggtcatcat
    28501 cgaaagcgcc ggattttccg aaggcggcag tgacggcaag gccatgcagc gcaaaatcga
    28561 cgaaatcgcc aaagcccacg gcattcggat catgggaccc aactgtctgg gaacactgga
    28621 tacccgcaag aaattctgct gtttctatgg cgtcaatccc agcctggtcg aaatgggccg
    28681 gatattcgaa gcaccgggta acatttcctt tatcatccag agcggcggcg ttgtcgtgct
    28741 ggtgattgag tccttttttt acgatctcgt cggcgtgaac aaggttgtca gtatcggcaa
    28801 caaatgcgat gtggatgaag cggatctgat cgattatttt cagacggacg aaacgcaggt
    28861 gatcgggctg tatctggaaa gcgtcacaaa tggtcgcaag ctgatggagg ctgccagaaa
    28921 atcgcgtaaa ccgatcctga tttacaaagt aggacgcaca caggaaggcg ccctggcggc
    28981 catgtctcac acggccggca tggccaacaa cgacagcatt ttcaacagcg cctgcaaaca
    29041 gtcgggaatg atccgtctgc aatccatcga cgaactgcat tccatgccta aaatgctgac
    29101 ggagatgccc ctactgaaag gcaaaaatat tgccatgttc acaaattccg gagcgtttgg
    29161 cagcatcgga gcggatctgc tggtggatgc gcatcttcag gtcgtgcgcc tgaaaccgga
    29221 aacgcaggaa aaattaaaaa aggcggggca ggttttcaat atcaagaatc cggtcgatat
    29281 cggtcctgcc ttgccgcaga cctatctcga tatttatgaa atccttcttt ccgccgatga
    29341 agtggacggc ctcctgccgc tgttgagcgt ctggcagacg tttgtaatcg atacccttct
    29401 ggagctcatg aagatgtgca ggcattatca gaaacccgcc gccatctata cgcccaacgc
    29461 catagccaag tccatcggca tccgcgccaa gcaccgcatc ccgatctttg aaaccgccga
    29521 gcaggcggtg cgggccttgg cagtttccgg ctaccattat gactccctgc agaaaagatt
    29581 tcccgcccgg gcaaagtgat gaattcctgg ggcaccgggg atgaattttt aaaagaattt
    29641 acccaggacg ttcatcatgg cattagcgat aacctgatgg ccttgttcat tcggatagcg
    29701 cgaatcctcc tgcgtaaggt cctggctgca aagattgtgt tcctcaaggt aagcgttcca
    29761 gcttctgtgt acggggatga attcacactt cagatctgaa gcaacaatcc gcagcgaccg
    29821 gtaaaagatg tgaacggcat ccatgtcatt gggattatca aacgtatgcg atgtcgccag
    29881 aaaaataacc ggctgcaatc gtttgcggct cagacgaatc atctgcacaa tattttcctg
    29941 aaattctgaa cgataaacgc actgaaaggc atcatccacg ccgaagtgaa tcagaagaaa
    30001 atccggctcg taccggtcaa tatcctgcgg ataggtctcg atgccgtcaa aagaggtttc
    30061 ccggtatcgg gcgcggttga tcacacacac cccttccgga agcagcgctt gggttaaaat
    30121 atcaacatag cttttcgtca cgccgtagcc ggcggcgatg cttccgccgc gaatgaccag
    30181 tttcacgatc ttcccccttt attctttgta acgtttctca ccggcgaaaa cgaccggatg
    30241 cggcccttca gtaatttcgg gccaggttct tgaactggtt caaaccgtcc agatatttca
    30301 catccctttc gtaaagttcc ttccatgatt tttgcgcctg ttgccgaccg accgtgctcc
    30361 tgaggaaagg acgcaacagg tgtgtccaaa acgttaccca gagaccccct ttgattctca
    30421 tgagacggga tggttcccac cagccgaaca tcttctgccg atgcagccag accagatact
    30481 gcagttgttg agtatcaaga tgccgcgttt taatattggc tctgaggccg ttgtacagtt
    30541 tgtaatcatg caggttcgtc accatgcctc tttccagcaa ctgctggcgc accggtgttt
    30601 tcggataggg cgtgagaatc tggcaatagg gaatatccgc gtcaagcgat ttcagaaagc
    30661 gataattatc aacgatatcg ttttcatcat catcgggaaa accgaaaatc agtccggcga
    30721 tcaccatgat gccgaactga tggcatatcc gaacggcttt tctggaaaga tccacgatat
    30781 cgcccttacc ggacgccatc agattttttt tcacgacatt ttcaatcccc agaaaaacgg
    30841 tttttacgcc cgcaagagac attttcctga ccatggtttc gttccccgca atggaaatgc
    30901 aatcggcctg aacgtgcagg ctcaagccct tgtaattttt tgcaatgatc gcatcgcaaa
    30961 gatcgatcac ccgttcggga tccaaaacca ggttgtcatc cacaataaag atggcgcggg
    31021 tgttatgctt gtaataaata tcatcgatat ccgccgtaac cctttcgatg ggaaacgtcc
    31081 gaaacgtctg tccgtacatc tcccgcatgc tgcaaaagga acaggtcctg gtgcaccctc
    31141 gcgaggtctc gataacctcg attttggaaa agacggcgtg atagccggat gtcaaacggc
    31201 gtttatcgcg tacgggaatc ttcaacctgg aaagatccag cagggctcct cttggattat
    31261 ggcaaaacgt tccccgacgt ttaaaagaca gggagggaat atcctgcaac tgatcgctgc
    31321 cttccagcgc attgaccaga cgccggaatg cctcttcccc ttccccgcgg atcatgaaat
    31381 cgatcagacc ggactccggc gatgccgcaa tttcttcata catcagcgtg gcgtgatagc
    31441 cgccgatggc gatttttacc tccggaagca gaagccggat aaagcggatg atttcaatac
    31501 aggtctcata ctgccaggcc atggccgaca aaccaaccag tttcggatgg atcttaagaa
    31561 gcaatcgttt cagatacccc gcaagatgcc tgcgtttccg gatcagatca accacgtaaa
    31621 cgtcatggcc ctcgtcaagg ttggcgccga tgctggcaat tccgcaattg ggcaggtgaa
    31681 atgcactctc atgcataatc atcggaatcg agtctggcat ggacaataat acaatcttca
    31741 tgttttctcg aatcttgttg caaatgcgta ttgctgttat ttaacactgc tgatgctttt
    31801 ggaagaagct gtccaacctg gaatcgtgct tagcgataga atcgtttatg ccggggccgc
    31861 tatacctgcc attctttttc ccattgcagc ttgtcttttt cattgatcag tctttttccc
    31921 gtaagaaaat aatcggagat ttccgaatgt ttctgaaggt attcccgatc tatctgttcc
    31981 aggagatctt tgagggtata gaagcactca atataacaat tgatctgcgc attggaaaga
    32041 gttgcccccc acgactgctc aatgatttct ttggtgacat aggcaaattt gacaatttcc
    32101 tgttcagtga gattgacttg catgcgttat cccccgggca atgagtaaaa atctttccgt
    32161 tttggatacc atagaaacaa cggactcatt tttcaagtat tattttgtaa tagccatgcg
    32221 cgttgttgtg aagggtaaac ggcatcagaa gaacggcgat gcagttcctc atggtatata
    32281 cattcaaaag acgcagcagc agtttcgtca gcatcgggta ttcaaaatat cgccgcgcca
    32341 gatattgaag tcgggcaaga ttgggcatga tggacgcgga caaatctttt ttctcatgga
    32401 tgcggaatcc ttccgcaacc gccatggcca gccagccgtc aatatcttcg aagtgatcga
    32461 cggccagtgc cttttcagtc atccgggccg cctcgattaa acgatccggc aaacggtgga
    32521 aaccgctttg ccggaatccg tcgtaaacaa taaatcgccc tccttttttc agcacccgga
    32581 aaacttcgga taatacctgc cgcacttttc gggcatgaca gatggattcc agttcaaaga
    32641 ccgtatcaaa agcaccgtcc gggaacatga tatcttgaaa gtctccgtac agaatggaga
    32701 ggttgtgtat gcgggatgct ttttttcgag caatcgccag atgctccccg ctgacatcga
    32761 ttccggtaaa atgagcgtgt ggtatttttt ttgccagata agccgaattg aatcccttgc
    32821 cgcatcccag ttccagaacg gcgccggccg gcaacggact gatgatctcg acaatttcgt
    32881 tgagggacgt gtaaaaaccc tttgcattga agacaccgtc ggaattgacg gccatatgca
    32941 tcgcgccgct gtaggaatgc acgtacttgt aaacgatccg gctttgccgg taataatcga
    33001 cgaccttttc acaatctatc gcctctccgg tgaggcaacg aacaggccaa acggcatcga
    33061 ttttctttaa ataaaaatcc agttcgttta aatgaacaag catcgccttt ttcataaagg
    33121 gcacccgtct gtcgtcaagc ctgtttttga tctaaacaat aaattttcta aacgatcacc
    33181 ggcccagacc tttttgatga ttttcgttcg atgtcttttg tgtaaccgga tatggttcaa
    33241 tggcctttcc ggcaaatctt cgaacagcct cgcctgcaac gatgccaacc gccttcccgg
    33301 tcatttttac acagacctgc cgggtttcgg tcaactcatc aaaacgatct tacttttttg
    33361 ttccgaccta tgaaccaaag gagagaaaac acgtcggagc gtgatttcac gaatgacttt
    33421 ttttcaattt aaaattgttc aggtaatcct ctaaagcgag ttgccacggc tgcatggcct
    33481 tgcccgtaat ttcaataaac gtcttgttgc ttaaaatact gtacgccggg cgcatggccg
    33541 gtctttgcag ttcggaagac tttatcggtt ccacaacgat atgaccatat cccgcgctat
    33601 ttaaaatcct gacggcaaac tgatgccagc tgcagcttcc tctgttggtc acatggacga
    33661 taccggttgc gtttttccgg atgaggaaag caacggccag tgccagatct tttgtatagg
    33721 tgggtgaacc gacctgatca tcgaccacag agagcgttga gggagcttcg gcgacggctt
    33781 tggccctgtt catggtatcg cggatgaatt cttttgcctt ggccttatcc agaatggctt
    33841 gaacaaaatt tttcccgttg attccgtata accacgagga gcggatcaaa aggtaattgt
    33901 ccgacaacgt ccgcagataa ttctcaccgg ccagtttcga tgctccgtag acattgacgg
    33961 gattgcacaa agcatcttcc ctgtacggtt ttcgggcggt tccgtcaaaa acataatccg
    34021 tgctgaaatg cagcacgcgg attttttttt gacggcaggc cccggcaata tttttgaccg
    34081 cttcggcgtt gacggcaaaa catgtttccc tgtccgtttc gcacccgtca acattggtgt
    34141 aggcagcggc gttgatcacc atatccggcg atacatcttt aatggcctga caacagtcat
    34201 tgtcagaaac aatatcaata tcttcctgat ccatcccgat gagttcatgc tcggcggaaa
    34261 gcctcaggaa cagatcgttt cccagcatgc ccttatgtcc cagcagcaat atcctcatgt
    34321 tcagtattta acctctttca ggaacaagcc gcaggccggc gctgtcgcac ccgcaatatt
    34381 tctgtttttg gattcaataa ttagcttcat ttcctcaggt tgtcgtttac cgcgtccgac
    34441 ctctatcagc gtgccgataa tgtttcgcac catgtacttc agaaatccgt gagactccac
    34501 ttgaatttca atcagccctt cttctccggt cataatttca atatcgacaa ttgtccgcac
    34561 acgatctttg acatcggtcc ctgctgcgca aaaacaggaa aaatcatgcg ttccgatgat
    34621 gagccggaca gccttccgca tgctcgcaat atcgagaggg taacgcacat gccagaaata
    34681 attacgaccg aggacgggtc tcaaacgctg attgcaaatt cgatatacgt ataccttact
    34741 tttgacgtcc cgcaaagcat gaaaatcgtc agaccgttct tccagatttt tgaccacaat
    34801 atccggaggc agcacactgt tgacgcccat aaaaatcttc tgaacgggca atgtcgtatc
    34861 acatttgaaa cttccgacct ggttcatggc atgcacaccg gcgtccgtgc gcccggaacc
    34921 gatgaccgac acgttttgac cggtgatttt tttaatcgcc tcttcgagca gttgctggat
    34981 ggcaatcccg ttatgctgcc tttgccagcc gcagtaggca gctccgtcgt attccaccgt
    35041 tattttaaaa ttgcgcatag cgattccgag tgatgattgt tttcaggcaa ctgacatcat
    35101 gtgtcgattc tgactctcgc gtcgagcgcc aaaacgccgg atccgtcggc taaaacacgc
    35161 accggattga tatccagttc acgaatctgc gggaactgat gcagcagatg gctgacccgc
    35221 tcaatcatcg cgacatatcc cgcaatatcc cccgctccct ttcctctcat cccctgtaaa
    35281 atcttatagg attttaatcg tttgactccg gcttcgattt catgacaatc cgccggacac
    35341 agcaggttgg cggtatcctg gaaaacctcg atatagatgc cgccgtaacc gaaaaagaca
    35401 accggcccga aagattcatc gaattttccg ccgataaaca tatcgtagcc gtcacttgcc
    35461 tgcttcatga cccgcacgcc ctgaaaatcg gcatgagcgt tgtagcggaa aaggttgctt
    35521 ctgatttgat cgaatgcttt gacggcttct tccgcatttt gaacatccgt gataacgccg
    35581 cccgcttccg atttatgaac ggcatccggt gacacaactt tcaacaccac cggataaccg
    35641 atcgtatccg ccagagcgag gacctctttc ccatttttcg ccacatccga tggagcgacg
    35701 tgaatgccga aacggctgag cagttccagg gactcttccc cccgaacccc ggcatgtcct
    35761 ttaatccatt tctgcgccgc ctcgatgtcc acaccgtcag gcaacgggta cccggtctcg
    35821 ccacgcgctt ttttcgcgta atagtcgtat tgttttttaa acgcgtgaac catttcgctg
    35881 gcgttgttga aaatcggaaa atccagattt tgtttgatcc gcgaaatggt cgccgagaga
    35941 ccgaagagac aaacccccaa cggcttgcct gacgacagaa tcgttccggc cacttccttg
    36001 gataaatccg tatggaacat tttataaaat acatcctcgc cgcgcggcat ttccggccac
    36061 tgcgtgacga acacggcgcc gtccacattt tcattatgca tgacggagaa aaagatttgg
    36121 ggataagcgt tcatgtcgta gatatccccc atatcgaggg gattggaaaa attgatgacg
    36181 ccggcattgg atgaattttt gagcttatca taaaaatcgg cgcccggatc ggcaaactcg
    36241 aaaccggagg cctcgcacaa atcggccatc atgaccgtga agccgcccgc gggagacatg
    36301 accatcatgc gcttgccgcg catcggcggc agctcgaaaa ccttagccat cgaaataaaa
    36361 tcgttgaact gatggatccg gatgataccg gaacgttcaa acgctgcatc aataatgtcc
    36421 tcattattgg ccagtgcggc ggtatggctc atggcggctt tccttccggc agccgtcgtg
    36481 ttcgacttga gaacaatcac cggtttgtca attttcgatg cggcggcgat cagacgatcg
    36541 ccgcgcggca cgctttccag atacatgccg atgactttgg tttccggatc ccgtccgaaa
    36601 tattccagaa aatccacttc atccagatcc agtttgttgc cgatgctggc aaatttcgcc
    36661 atgccgacat tttcgtccgt catcagattc caaagcatca gccccacgcc gccgctttgc
    36721 gaaatgatcg agagtcctcc cttgggcggg ctgtacgagg gaacaaaagg caggcacaac
    36781 ccgttggcgg tattggccac cgtcacaccg ttgggaccga cgaaccggat cccgtattct
    36841 ctggccttct gcaaaaccag ttcggacaac tttctgccct cttcgcccag ttcgttgaaa
    36901 ccgccggtgg gaatcgccag ccgcttcacg ccggcccggc cgcaggaatc catcacatcc
    36961 ggaatcatcc gcgcgggaat gaggacgtac accagatccg gaaccacggg caattcctgg
    37021 acggatttgt acattttaat gccgtccaca tgggaatccg cggcacgcgg attgatcccg
    37081 aagattcgcc ccttgtaacc ccatcgaatc aggttttcca gaacgaggcg gggaatattt
    37141 tgcggcttcg atgacaagcc gatgatggca atggattcag gataaaataa tttttccatg
    37201 acaacctcca gactaaaaac ggatcacaaa atcacctgca ggcctgacat cacagcgcct
    37261 cggccaaaac catggcgcag caccctgcct tgaatcccgg tatgcgtttt gcaaaacggt
    37321 tcaacctgct aacggcaacg ttgcgagtat aggaaaagcc tgtttttctg tcaatcaaat
    37381 cttgggcaga atatcctttc cggcggtgct ctgtcggaca aattccgtca acgcttccgg
    37441 gtgttgctcc gacaaaaaaa tgagacgtcg ccggatagaa tcatgcatgg ggctgtccta
    37501 aaattttttg taggatagat agaatatgct gatgagtttt aaaaaaccat atcccgcgca
    37561 atacgaaaag cttctggcgc tgaaaaacgg caaaaacgtc ttcgtccgtc cgatccggga
    37621 caccgacgga aaactgcttc tggaactgtt cgacaaactg tatcccgatt ccatctaccg
    37681 gcgctttctg acgccgctca gaatgatccc ggagaacctg cttttcaagc tgacccatgt
    37741 caactatgac agtgatttcg ccctcgttgc ggtcattccg gaaaacggca aggatgccat
    37801 cattgccgtt gcgcgttacg gttacgatgt cagggaacag gttaccgact ttgccattct
    37861 ggttcgcgac gactggcagg gactggggct ggggaagcgt ctgctcaagg atatctttgc
    37921 catcggcagg gaccacggcg tctcctgttt tgtatccctc attgattcca acaatcatgt
    37981 catgaaagag ctgatcaaaa gactggatta cccggtaacg tattcctaca gaacgggagc
    38041 ctccaaagtg cagatttttg tctgaggcaa tatatgcttg cacaaacgcc gcgccggata
    38101 cgctcatcat ttcttcttgc cagttttcat taaaaaagct agtatttcct ccaatcataa
    38161 aatatctttt caacatgact gcttcatgtt ccatgtgtag ggatcccaaa gactggagaa
    38221 ttgtatgagc gaagatgtac agaagaattc aacgcaggaa aaaatcaatc attttgagaa
    38281 aaagaatcgg accctcaaac agatgggcgg cgagaaacag atcgcccggc agcatgaact
    38341 gaaaaaattg acggcccggg aaaggctgga tttatttttt gatcaggaca cctttcagga
    38401 agtgcagctt tttgttgaac atcgatcgac gctgttcggc ctggacaaaa aagatattcc
    38461 cgccgacggc gtggtgacgg gcttcggaaa agtcaacggc cgcaccgtat ttgttgccgc
    38521 gcaggatttt acagcctccg gaggttcgct gggggaaatg catgcgaaaa aaatctggaa
    38581 ggtcatggag atggcccttg ccgcgcagaa accgttcgtg tcgctcaacg attccggcgg
    38641 cgcgcgcatt caggaaggcg tcccttcact ggaaggctac ggcggaattt tttaccgcaa
    38701 caccctggcg tccggttata ttccccagat tacggcgatt atgggcccga cagcgggcgg
    38761 cgctgtctat tcgcccgctc tgtcggactg ggttttcatg gtcaagggaa gcagcttcat
    38821 gtatatcacc ggccccgatg tagtcaaggc ggtcatcggc gaggaaattt cccatgacga
    38881 actgggcggc gctctggccc atgctgccaa aagcggtgtc tgccacgtcg tcaccgaaag
    38941 cgataccgac tgcattgaaa aaatcaaaaa gcttcttgcg tatctgcccg acagttgcca
    39001 tgcgcccctg cctctgtccg attgcacgga ccctgcggac cgaacctgtc cggaactgga
    39061 ttcgatgatt cccgacaaaa gcaaccgggc ctatgacatg aaaaaaatta taaagtccgt
    39121 cgccgataac ggcgagctgt tcgaactgct ggaactctgg gcgcagaata tcatcaccgc
    39181 ttttatccgg gtaacaggca gaccggtggg cgtcatcgcc aacaatcccc gtttcgctgc
    39241 cggtgtcctg aatgtggacg cctccgataa agcagcgcgc ttcatacgct tctgcgacgc
    39301 gttcaacatc cccttgctga cctttaccga tgtgccgggc tacatgccgg gaacccatca
    39361 ggagtgggcg ggcattatcc gtcacggcgc aaaactgctt catgcctatt cggaagccac
    39421 cgtgccgaag atcaccgtca tcacccgcaa ggcctacggc ggcgcgtaca tcggcatgtg
    39481 cagcaaacag ctcggcgccg attacgtcat ggcctggccc acggccgaaa ttgccgtcat
    39541 gggcgccgaa ggagcctgca acatcattta ccgcaacgac attgccgggg ccgacaatcc
    39601 gactgcccgg cgggcacaac ttgttgaggc ctacgaacag cagttcaaca atccctattt
    39661 tgccgcatcc atgggtatca tagaagaaat catccctccg cgcgataccc gaaaaaaaat
    39721 cattgctctg ctggatgcgc tggggaataa aaaagtaacg gggctttcca aaaaacacaa
    39781 caatatcccg ctgtagccgg tcattcttgc cctgatgttt tgaatgcttc aggaggacgc
    39841 catgaaaaag atttttgctg cggttgcact catcgttgtt cttttgacgg cttatttaac
    39901 cctctggcct gttccggtcg atcccgtgca gtggattcct cccctgtccg acggatattc
    39961 aggcccccat gccgtcaaca ccggacttgc cggcctgcgt atgatttcac tgggccggga
    40021 agaaggcccc gaacacatcg tcatcggcaa agaccaaaag ctgtatacga ccgtagccgg
    40081 cggaaaaatt ctgcgcatga atcaggacgg gacgcaacag gaggtctatg ccgataccgg
    40141 cggacccgtt ctcggttttg attttgacgc ccgcggcaat atcgttgccg ccgacgttga
    40201 caggggcctg gtgctcatca gcccggataa aaacatcacc gtattaaccg ataacgtcaa
    40261 cggcgacccc attcgttttg ccgatgcggt cgcagtgtca caatcgggga aaatttattt
    40321 caccgacgcc tccacgcgtt ttgcgcccaa aaaatgggga ggcatctttc cggccagcct
    40381 gatcgacatt ctggaacagc gctcgaccgg acgcgttttg gaatacgacc cggcagcaca
    40441 gaccacccgg attgtggcca aaggattcag cttcgccaac ggcgtggtgt tgagcgaaga
    40501 tgaaaactgg cttttcgtca atgaaacagg aaaatatcgc gtctggaaaa tttccgtaac
    40561 cgcacaggat ttggacattg cctcgcctgg ggaatctgcc gtcatcctgt ttgagaacct
    40621 gcccggttat cccgacaacc tgatgcgcgg tcttcacgga aagatctggc tgggatttgc
    40681 caagccccgc aatcccgcca ttgatcaacg cgtcatcacc tccacgcctt ttatgcgcaa
    40741 gctgacctac cggctgccga aatttctgtg gccgcttccc aaaccctacg gccatgtttt
    40801 ggcctttaca gaagacggcc ggatcgtcgc cgacctgcag gaccccggcg gtcgctatcc
    40861 ggagacaacc ggtgttacgg aaacagccga caggctctac atccagagcc tgcatgccaa
    40921 aggcatcggc tggctgccca aagataatgt cttttaatct ttcgaggaat cccgtgtatc
    40981 atattctggc agatatcctt gttgcggctc attttctgtt tattcttttt gttgtattcg
    41041 gcggtctgct cgtcatccgg aaaccgtcct tcgcatgggt gcatctcccc gcggcactct
    41101 ggggggctgt cgtggaaatt ttcggctgga tctgtccgct gacgccgctg gaaaatcacc
    41161 tgcgaacgct tgccggcgaa tcggtttacg gcggtgattt tatcgcccgg tacctgctgt
    41221 ctttgatcta tccggaaaat ctgacaccgt ccattcagaa aggtctcggt gcagtggtaa
    41281 ttgtcatcaa tctgattttt tattttatcg cttttaaaaa gaaccgcgct cgccgctagc
    41341 gctcgaaata cttcagccgt cttaaagccg cgttccgccc cagcacatcg aaaattttat
    41401 ccagctccgg cccgtgaagc aaacctgtta atgctgcgcg caccggcatg aacaacgccc
    41461 tgccctttgc cccggttgcc tccctggcaa atcggacggc ctctgcataa agattttgat
    41521 ttccggcatc ctgtttttca agatatgagg caaatgcctc gaccactttc cgggcgggtt
    41581 cctgatccag caggtctttg gcctctgcgg aaatcctgta ttgactgtca aaaaaaatgc
    41641 tgatgtgatt tttcaggtct gcaagggttg tcaaatccgt tttcaccagt tccacagcct
    41701 ttcggaacca ttccgggttt tgcatttcgt tttgatacgc cggttcgtca agaaacggtt
    41761 tcagcctctc caccagatcg tcggttcttg tgtttcggat gtaaatcgca ttgagccagt
    41821 ggagcttttc ttcatcgaaa acagcgccgc ttcgggaggc tcgttccagc gagaagtctt
    41881 ggatcatatc gtcacgggat aagacttccc gggcgccggt gaatgaactt cccagaagcc
    41941 ccagatagtt gatcatcgcc tccggcaata tgccctgcct gcggaattct ccgatcgaaa
    42001 ccgaaccgtg ccgtttgctc agcttggtcc gatccctgcc cagaattaaa gaatggtgag
    42061 caaataccgg ggaatcaaat ccgaaagccc gatagagcat gatctgcaaa gccgtattgg
    42121 aaagatgatc ctcgccccgg atgacatgtg tcatatccat cagatgatca tcgattacgc
    42181 aggcgaaatt ataggccggc atcccgttgg aacgcacaat aatgaaatcc cccatatcct
    42241 ccgcattgaa tttaacattg ccgcgtatta agtcttcaaa ttcaataatt tgtgactgaa
    42301 ccctaaagcg atacgaaggt tttcttcctt ctttttcctt gcggtttctt tcttccggcg
    42361 acaaattgcg gcacttgccc atatatcgcg gcatacgttt gcttaaaatc agcgtctggc
    42421 gttcttcctc cagctcctct tcggtgcaat agcagggata aaccgcgccg gctttcatta
    42481 atttctgcag gaattctctg taaactgcga cgcgttcgct ctgccggtaa ggcccgaaat
    42541 gtcctccctt ttccggtccc tcatcccaat cgagacccag ccatttcaaa tcatcgtaca
    42601 gattggtttg atacgaaagc gcgctgcgcg atgcatcggt gtcttctatg cgcaggacga
    42661 aggaaccgcc gtgatgcctg gcaaacatcc agttgaataa tgccgtccgc gcgttgccga
    42721 catgaagctc tcccgtcgga gaaggcgcaa atctgaccct aggttttcgc actatttctc
    42781 atcctttctg ccagcgaagc aacggcaaac accgccacgc cttctctgcg cccggtaaag
    42841 cccatgcctt cgttcgtttt cgctttgata ttcacgcatt cttccgaaat gttcagaaca
    42901 ctggcgatat tggcgacaat cgcatccgca tagggcgcca gtctgggaac ttccaggata
    42961 accgtggtgt cgatattctg aatacggaag cctttcccgt ccaaaatttt tttgacgttt
    43021 ttcagcaaaa tcatgctgga gatatctttg tactgcggat cggtgtcggg aaaatgcctg
    43081 ccgatgtctc ccgcgcctgc ggcgcccagc agggcatcgc agattgcgtg gatcagggca
    43141 tcggcatccg aatggccttc gagtcctctg tcgcagggaa tttccacacc gcccagaacg
    43201 agttttctgc cttctgtaaa ccggtggctg tcataaccga agccgctccg gatgccggcg
    43261 gtgtttcgct ttttcatgaa tgcctccgca accagcatat cctccctggt agtgattttt
    43321 atattgtcat aggaaccttc gaccattttg acctctttgc cggtcctctc caccaacgcc
    43381 gcatcatccg tcccgtaaaa attttcctca taggctctcc ggtaagcgtc tttcaatata
    43441 tcgaagcgaa acgcctgcgg ggtctgcgtc agccacagat tttcccttgg caccgtggat
    43501 tggatcatgt gatcgtcttt tgttgcctta acggtgtcct tgacctgcac gccggtcacc
    43561 gccgctccgc aggccctggc ggcagccacc accctgccga tcatttgatc cgtcacaaag
    43621 ggacgcaccg cgtcatggac aatgatcagc ccggcattcc cctggatcgc gtcaaggccg
    43681 tttttgacgg aatcctgtct ttcccggcct ccggcaacaa cggcaaccac tttgctcatg
    43741 ccgtattttt caatcaggtc ctggcggaca tgcaccacat cgtcttgcgg caacaccagg
    43801 atgatttcgt caacaaccgg agagtcctga aaaaccttca gcgtatgaac cagtacgggc
    43861 agcgcatcca gcagcagata ctgcttggcg acgtgggatt taaatcgttg ccctgccccg
    43921 ccggcaggaa tgatgagcac cgttttcatg gattgttccc cgttattttt catgaagctg
    43981 atagcatttt tattatcaaa atggaatagc gataaaaaaa cccgggagat tgatcccggg
    44041 ttttaaaaaa accgatgacg cattcggctt aagccacttc cttgagttct gaaaaaatca
    44101 ttcttcccgc agtggtctgc agaacactgg tcacaaccac ccggacattc atattaagca
    44161 ttttctgagc gttgtccaca ataatcatgg ttccgtcatc cagataggca acgccctggc
    44221 cggcttcctt tccctctcgc acgattttca cggtcatctg ttcgccgggg agcaccaccg
    44281 gcttcatcgc gttggccagt tcattgatgt ttaacactct gatgccctga agttcggcca
    44341 ccttattgag attataatca ttggtcacaa gcttggcgtt gagctgtttg gccagagcga
    44401 ccagttttcc gtccacgcct ttgatcttgg gaaaatcctg atcgacaatc tcgatgttga
    44461 cgttggcact tttctgcaga tgattgagga catccagccc gcgccttccc cgcagacgtt
    44521 tcatcgaatc ggacgaatcc gccacctgct gcagttcatt caaaacgaat cggggaacga
    44581 tcaattctcc ttcgatgaat ccgctgtcgc agatatcggc aatgcgtcct tcaatgatga
    44641 cactggtatc cagaatacgg tgatcgtggg cggttttgga atcgatgccg ccaaagctga
    44701 attcttcgat ttttttcgaa cccagggtga ggcccagata actgaatatt cctgtaatca
    44761 gcagataaat ccagggaagg gcctgctgat tctccctgat tttacttaca aaatttaatc
    44821 cgaaactaaa tattaatgca atcaggagtc cgacaatcat gcccgcgacg ccgccgatga
    44881 taattttgag agataattga cgaatgtgct gttctatttt tatgacagtc aaggcaatcg
    44941 ttaatccgat aataaagccg gcaatggatt taaggatcag aaaagaatcg gaagacgtat
    45001 aaaaggcgat ggcataaccg ctgatggaac atgccattat taatataatt ctgataatca
    45061 accgtttcac ctcctttctg tgaatcatga atgttaaaaa aatttgttta taaaaaattt
    45121 atttaaaaaa taatataagg acggaggaaa aatgacggta tccctaaaaa aagaaagtaa
    45181 cctgtctttt ttcaattttc aaagagcctg attgactatg aagatgattt ttctactgaa
    45241 cggtaaaaat cttctgcaga ttatgctcga cttcagattc ctgcgcgttg gtggccacgg
    45301 atatttcttt gaccagaaga tttttggctg tatccatcat ctttcgttcg ccgaaggaaa
    45361 gatttttatc gctttttaaa ataaacaaat cgcgcagaac ccccgcaatt tcaaataccg
    45421 aacccgtctt gattcgctca agatattcgc ggtaacgttt gttccaggtc tgcttatcga
    45481 tggtgacatc tttgttgcga agaatttcgt acaccttggg tatttccttt tccatgatga
    45541 tttcccgcaa gccgacggcc ttgacgctca tcgtgggaat catgattttc atgccgttcc
    45601 ccatgatctt catcacgtaa aaagactgtt ttttgcccat tacttcttta ttttcaatgg
    45661 cttcaatgac tccaacacca tgtgctggat ataccgccaa atcgcctact ttaaacattt
    45721 tatttttcgc ctgcctttcc atatcagagg gttattatac cactacttga actattagtc
    45781 aataaccaat ccgtatttaa gtaatacttg aatatttcat ggctaaaaaa ccataattta
    45841 accgtttttt aaacacaaca tgaatgatta aatatcggat ataacttata aaaataaaaa
    45901 ggagtcactt tttttaaaaa aagtaaactc ctgaattttc ctggaggcgg cacccggact
    45961 tgaaccgggg aataacggtt ttgcagaccg ctgccttagc cacttggcta tgccgcctta
    46021 aaaataaaaa tggagcgggc gaacggattt gaaccgtcga cgtctacctt ggcaaggtag
//
LOCUS       DAOOLB010000011        44220 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_609_length_44220_cov_42.772059, whole genome shotgun sequence.
ACCESSION   DAOOLB010000011 DAOOLB010000000
VERSION     DAOOLB010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 44220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 44220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44220
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_609_length_44220_cov_42.772059"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1811
                     /locus_tag="PLF77_02725"
     CDS             <1..1811
                     /locus_tag="PLF77_02725"
                     /inference="COORDINATES: protein motif:HMM:NF014731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HPC07798.1"
                     /translation="IGTSPLYAIKACFYGQHALAPDITNILGVLSLIFWSMFVIVSIK
                     YVIFLLRADNRGEGGIFALVSLLNVSKKSFSPLVQSVLVLAGILGAGLLYGDGVITPA
                     ISVLSAIEGLEVATHAAAPFILPLTCVVLILLFVLQRKGTSNIGKVFAPIMVLWFLSI
                     GIFGIMQIVREPSVLRALNPLYAVEFFLNNGMHGVVVLGSVVLCITGCEALYADLGHF
                     GRNAIRLSWFSLVFPSLLCNYFGQGALLLAHPEMNVNPFYKIVPEILLYPMIGLSTAA
                     TVIASQALISGAFSLTQQAAQLGLCPRVRIIHTSSDMQGQVYIPSVNYALMMACIAVV
                     TGFRESGALAGAYGIAVTGTMIITSILYFLVLTHHRGWPLRKVIPLVGLFLLVDIAFF
                     TGNFFKLADGGWLPLLIAAIIAVAMTTWKKGRNELYRNLVGARFPLESFLSELPKRHM
                     PRVPGTAVFMTLSPVGTPPTLLHNVKHNHILHETVVLLSIITKDTPAVPAGERIKLAD
                     LGQGFYRVEAFYGFMQKPNVPQLMKILAQYGLVTDPMTTTFFLGKETLLTGGKSKMMK
                     WRKSLFAFMSRNAGNPTSYFGIPANRVVELGTQIEL"
     gene            complement(1929..2468)
                     /locus_tag="PLF77_02730"
     CDS             complement(1929..2468)
                     /locus_tag="PLF77_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPC07799.1"
                     /translation="MISRTAAVLVIIDIQGNLAQAMFGKENIFANAVKLIKAFKTLNL
                     PIIVTEQIPGKLGKTLPQIADEIEDFNPIAKESFSCWGEKNFKERLESLSRRHVVLLG
                     IETHVCVYQTALDLLSEGYNVHLVADAVSSRTPENRQIGIKAMKSAGAHIASTEMVLF
                     ELLRTAADPKAKEFFKIVK"
     gene            complement(2483..3283)
                     /locus_tag="PLF77_02735"
     CDS             complement(2483..3283)
                     /locus_tag="PLF77_02735"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC07800.1"
                     /translation="MLDVSQFSLKDKVAVITGGGRGIGQAIAFAFARAGAKVVVTSRK
                     APDLEATANEIKAFGGDAYPLPAHLGKSEEIKKMIDAVMAKYSRIDILVNNAGASPAK
                     GSVLECDERLWDKLMDINLKGAYFISQAAANIMVKQGGGKIINIASIDGHNPEPGLSI
                     YAISKAGIRMLTKAFASELVRYNIQVNTIAPGPIATKMLKSNWEFLPPEDAQKVKELV
                     EKALPSGRIGDPDEIAGAALYLASDASSYTTGAEIVIDGGVLLGMHPS"
     gene            complement(3395..4858)
                     /locus_tag="PLF77_02740"
     CDS             complement(3395..4858)
                     /locus_tag="PLF77_02740"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HPC07801.1"
                     /translation="MRTREQYIERLRKMRRNLYHDGEKIDRDDEIQAPVLNVMGLTFD
                     AVHDPELKDLCTATSHLTGETINRFCHVHQSTDDLHKKQDMTRALCRKAGMCIQRCMG
                     TDALNAVNAASFEADKVNNGNTQYHKNFLKWLERFQKEDLVGSCAQTDMKGERLKRPA
                     EQTDPDMYVHVVEKKKDGIVVRGCKLHISEASISDEIIVVPTRALGPDEKDYAVAFAV
                     PSDYEGVKQVVSIHNQHKRKHIKRGIDIGYSDSYIIFEDCFVPWERVFLCGENDHGGM
                     CALLFALFHRHSYSGCKPAIGDVFLGLAALAAEYNGIEKTPHVKEMLAEIIKVSELGY
                     AAGYTASSMGKPEVIIPGLGMCPYGPGSYIPNSIYCNVGRCLTGEAVFHEQELICRIA
                     GGLPSTFPYEGDLTHPDLKKVLEKYLNRNPKVPVEDQIKFWMTLGDYTIGTMAGVMNY
                     GNYHGGGSPIMEQIAITSQYDIKDRKKLIKRLAGIGG"
     gene            complement(4890..5597)
                     /locus_tag="PLF77_02745"
     CDS             complement(4890..5597)
                     /locus_tag="PLF77_02745"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC07802.1"
                     /translation="MTDKNRPKTRKDRIMDAALRIFAEKGFQNATITEISKAAGVSEA
                     TIYEYFGTKEDLLFAIPEKITNDTFEESSKVIPYIKGVEGKIRAILLFYVKLYQSNPN
                     YSALVLLQLMSNRRFRQTPAHAAIRTSAHTLLDCIREGIADGTFKKDSNPYLIRSVFM
                     GTIEHLFIHWHMQGMPKKKPGIAEMLDALLETVFDGIRARKEDSGTTLYLKLDDAGVL
                     EKILKKGRETSSKEKIR"
     gene            complement(5904..6194)
                     /locus_tag="PLF77_02750"
     CDS             complement(5904..6194)
                     /locus_tag="PLF77_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07803.1"
                     /translation="MIGFKYCGGCNPVINRAQLVQDIQRRLSAEFTLATDQSPTQWDI
                     GILVCGCLSACADKPDFRNLARRWIIIAGNSVDYGDAPEKDLAEIVLNKLKL"
     gene            complement(6236..7687)
                     /locus_tag="PLF77_02755"
     CDS             complement(6236..7687)
                     /locus_tag="PLF77_02755"
                     /inference="COORDINATES: protein motif:HMM:NF023222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HPC07804.1"
                     /translation="MGLKTADQYEESLRKMNLKVYLLGEQVKCPADHPIIRPSMNSVK
                     MTYALAQSPEHEDLMTAKSHLTGEKINRFCHLHQSTEDLIKKVKMQRLLGQKTGACFQ
                     RCVGMDAINAVDSVTFEMDKKLGTQYHDRFIRFLKMVQEEDLVVDGAMTDPKGDRSLP
                     PSRQTDPDLYTHVVEKRKDGIIVRGAKAHQTGAVNSHWILVMPTIAMTKDDADYALSF
                     VAPADADGISYIYGRQSCDTRKLEGCEIDVGNRQFGGHEALMVFDNLFVPWENVFMCG
                     EYEFSGLLVERFAGYHRQSYGGCKVGVGDVLIGAAALAADYNGAAKASHVKDKLIEMT
                     HLNETLFSCGIACSAQGHRTASGTYLIDLLLANVCKQNVTRFPYEIARLAEDITGGLM
                     VTMASEKDLRHPEIGKIVEKYFKGIASVPTEDRCRIMRLVENITLGTAAVGYRTESMH
                     GAGSPQAQRIMIARQGNLEHKKKLAKEIAGIKD"
     gene            7894..9159
                     /locus_tag="PLF77_02760"
     CDS             7894..9159
                     /locus_tag="PLF77_02760"
                     /inference="COORDINATES: protein motif:HMM:NF033594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HPC07805.1"
                     /translation="MAERDMVVMSLGELKRLKLIQSAIDRQISQKQVSGILRLSERQI
                     RRLVRAVREEGDKGIINRSRGRPSNRGLSAGTKENVIALYKEKYSDFGPTFATEKLWE
                     RDGIRLSDETLRNWLIGAGLWKKHRKRSAYRQWRPRRSCFGEMVQMDGSHHDWLEGRG
                     PKLVLMGYIDDATNTIYAKFYDYEGTLPAMDSFKGYVKKYGLPMSVYLDRHTTYKSSR
                     KPTEWDEASDLKFLSQFERVLKELGVEVIHALSPQAKGRVERLFGVLQDRLVKEMRLH
                     GIKTKDEANGFLETYLPQYNEKFRVCPANETDVHVRPPRHFNWDKYLCIKDQRTIRND
                     HTIAYNRKLYQLEENDSKKVHIEKRRDGSLLIISKGRALKYKEITERPKQMVAVKKDL
                     RAYNRPPKPSKDHPWKQRWKTWGTTPTVL"
     gene            9406..10131
                     /locus_tag="PLF77_02765"
     CDS             9406..10131
                     /locus_tag="PLF77_02765"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPC07806.1"
                     /translation="MSIMNWTKDETVAIITMTNGENRQNLTFAKTLNAMLDEIIADKT
                     ITALIITSNDPKNFSQGIDVEWIMGQMQKNEHQPVTDFINEMDVVFKKLLLYPIPVIA
                     AINGHAFGNGSIIACACDFRFMRSDKGFFCFPEVNISIPFRWGMNAFVKKAIPIHLLS
                     QMQLTGNRFTAPELEKHNIIVKACANQEELMSSAIAFAKTFQKKRTIFGELKRRLYAD
                     IIKVLDDDFMPPTSKITSLVMMD"
     gene            10172..11155
                     /locus_tag="PLF77_02770"
     CDS             10172..11155
                     /locus_tag="PLF77_02770"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPC07807.1"
                     /translation="MKTAITEMFSVKYPIICGAMMWLCRPVLCAAISNAGGLGNLTAG
                     NYPTEDEFRDAIRQTRKLTDKPFMVNVTILPSVHVTAEHHKMYLRVCAEEKVAGLEIS
                     GTPLDKAVGKEFIDILKKAGVKLFHKVGSVRHAVHAEKTGYDGIYAAGIEEGGHPLND
                     DVTTMVLTPRIAETVKIPVVTVGGVADGRTLAAALALGAQGVMMATRFVATKECEVHD
                     NIKQEIIQRQEFETALICKSIGLQGRAIRNKLVEDVLAVESTGGGLEKLIPLIAGERV
                     KKAWEFGDVDSAPMMMGQSVGLVKDIPTCKELMERLVAEAQADIERVRNIF"
     gene            11219..12583
                     /locus_tag="PLF77_02775"
     CDS             11219..12583
                     /locus_tag="PLF77_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HPC07808.1"
                     /translation="MSGVTTKETALATKLSKPEPRDETVPGSNAHGRFVSREIIALMG
                     KDDITSVSLGDHIEKTMTILFADIRGFTSLSEEMPPRENFTFINAYMKCMNPVIARHG
                     GIINKYIGDAIMALFPADADDAVKCAIGMLRQLVAFNRSIKKTGSSRIKIGIGLNTGL
                     MMLGIVGGEHFMETTVISDAVNLASRIESMTKDYNVPFLISEHTYYSLFGASEHNTRF
                     VDRVRVKGKAQCQSVYEVFDADPKSLRTAKQKTKADFEEALAFYHFKDVPKATKLLEK
                     LLRRVPGDTVAKVYHERCLRFLKTGIHESSGEFDLAISWNKSMAIDHSLIDEQHRDLF
                     IHTREFVEAVKTSKNYSQLRNVLEFMDKYIQVHFRTEERLMAQKNYPFLQMQVDQHRR
                     FSRYFDGLRNEIKRNFETNRIFILFRIQVLIVDWLINHTSKLDLHFGKFLRYRKGRGL
                     KSLS"
     gene            complement(12607..13011)
                     /locus_tag="PLF77_02780"
     CDS             complement(12607..13011)
                     /locus_tag="PLF77_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07809.1"
                     /translation="MTQNKIVAHYQDGNLFKGITNDFSPNKELFHLFERAAQEDNRPL
                     TLKMDGLKGLFYVKDYDGNPSYHEQKEFDPSRPLAGRKIKVIFKDGELMVGTTNGYQP
                     NRPGFFLVPADANSNNERCFVVSSAATEIAFI"
     gene            complement(13049..13372)
                     /locus_tag="PLF77_02785"
     CDS             complement(13049..13372)
                     /locus_tag="PLF77_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07810.1"
                     /translation="MKNHENYVKGILEQNPDAEKLKELLAYHDKQIKWIQHERLVHLI
                     VMLFVCLFTMMIFGFAVIRPSLSVILLFVILLILSAAYLLHYFRLENGVQKWYVIAGE
                     IRRQF"
     gene            13562..13975
                     /locus_tag="PLF77_02790"
     CDS             13562..13975
                     /locus_tag="PLF77_02790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPC07811.1"
                     /translation="MVNKVILIGRLGKDPEVRYTPDGTMVTNFNLATDEQRKDKNGEK
                     IQKTEWHKIVTWGKLAEICGNYLVKGKLVYVEGRIQTRSWEDKEGVKRYTTEIIASDM
                     RMLDSKGQTKSGETSFDADAANVNDGSVPLDDVPF"
     gene            14125..15690
                     /locus_tag="PLF77_02795"
     CDS             14125..15690
                     /locus_tag="PLF77_02795"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC07812.1"
                     /translation="MKNINVLMIQPKTPQTFWSHDHTIKALGFKATMPPLGLLTVAAM
                     LPQNFNVKLVDLNIEDLDMRDVDKSDMVFLTGMIVNRESFMEMVHICQKKGKIVVAGG
                     PFVQSVYGTKGSNDLEISKIDHLILYEAENNLPQFLDDYVQGKAKRVYDNNEKPSLTQ
                     TPIPRFDLIRPRDYVSMALQFSRGCPFNCEFCDIIQMFGHIPRTKTPEQFMGEVEALY
                     STGYRGRLFVVDDNFIANRKKVKELLRLLAAWQEERKYPFLLFTEASIDLAKDDELLG
                     LMRRSGFTSVFIGIESPDEKTLRATNKNQNVTCDMNAAIAKVQKAGIEVMGGFILGFD
                     TDADDIFDSQLEFIRKNSIVQSMVGLLAAMPHTDLYNRLEREGRLVLGVNHSGNNVDT
                     DLNFIPKMPVKQLIEGYRRVIKETYSPRNYFARASTLLKKFPDMRMSKLKYAEPWLKK
                     LYMSKMTSHPNKSKIVFEIMKLVFKPFGIYGLVFIIKSLRFGLLSMPVAIELTFRGLH
                     YMSVAREIENAAD"
     gene            15919..17814
                     /locus_tag="PLF77_02800"
     CDS             15919..17814
                     /locus_tag="PLF77_02800"
                     /inference="COORDINATES: protein motif:HMM:NF016915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPC07813.1"
                     /translation="MKRMIPLFLLLVCMTLAGCAGVKVSFVGSGDYMSLRRGDILTTG
                     ELSTYTGASLQVVGIDKKVCDKDASGCRKALMETIGLHSENRLSALAELWLKDAMELE
                     KQFIESGHIEATLNAYLETARYSYAYLFLTKRTPNMRALEDRQTQVRDYYNYAVEKTL
                     TTLFEYYRKRLPPDFNFEKDTHFESGQWHITIRNEDLRLAEEHIFPEDLIPASSLSFA
                     GLRNQYRRDGLGAELVAVTSTRVVSRQSAEMPFSETPFPPVTAVMSFPGNTWMDVLKT
                     NEVVITGYDPYSRESFKLVGKEVPLAANFTSGYGLWLARSGFAAQSLLTLIGKGDVLE
                     MPRVYLMQPYNPQRHIVIMLHGLASSPEAWINLANEVLGDETLRKNFQIWQVYYPTNL
                     PIAFNHYEISKAITQTLKHFDPEGKARASQKILLIGHSMGGILSRLMVSSSGDRLWGA
                     LLKKYPLEGRRLERVRRELGPYMSFEPLPQISRAIFVAAPHKGTPVAEIALVRWIANF
                     IKLPFSVLGRFREVAQLLVDPGSANPVSLTRSFNSIDNLSNRDPFIQLTSTLPVSSRV
                     RYHSIIGNNTPKKPLAESSDSVVPYSSSHLEGAVSEKVIASGHSVQEMPDAIIEIRRI
                     MHLHLKE"
     gene            complement(17980..18948)
                     /locus_tag="PLF77_02805"
     CDS             complement(17980..18948)
                     /locus_tag="PLF77_02805"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07814.1"
                     /translation="MIKMKEIKAKNMNVNKFIDDKVRDIRQAVGDGFAINALSGGVDS
                     AVVTALGHKALGKRLKTYIIDNGIMRENEPAKVAAAFKKLGIQVDVFDASRAFFSALK
                     GLTDPEEKREALTQTFYKNVFGKLVVKSKARYLLQGTILTDVDETVAGIKRQHNVFEQ
                     LGIDPQKAFGYKILEPLIELRKDGVRKVGRGLGLPEDMFARIPFPGPALAARIIGEVT
                     PARIALVKKATAITEAALKDVKAFQYLAILHEDRVTGMRNNKRVFGNQIEIRCWNSLD
                     ARTARPTRLSYAILEGIAAKITNEIPDVVSVTYNITSKPTSTIEAI"
     gene            complement(19045..19422)
                     /locus_tag="PLF77_02810"
     CDS             complement(19045..19422)
                     /locus_tag="PLF77_02810"
                     /inference="COORDINATES: protein motif:HMM:NF024636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HPC07815.1"
                     /translation="MTSRRAKIGLGYHLAAFAAVNAVLVWINLDTSPEYFWAKWPLAG
                     WAVALSYHAFSVFSSLIKAHKGFYYHLFSFLIINAFLIFINFDLYPQYLWFKFPLIVW
                     TIMIVFHGWRVFSERQKAKAVAA"
     gene            complement(19505..19717)
                     /locus_tag="PLF77_02815"
     CDS             complement(19505..19717)
                     /locus_tag="PLF77_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07816.1"
                     /translation="MKEKHAIVKLVFGCIDDVVSTTESIHKSIAENVKGVSRSGFAEG
                     GKRKHVYDTIKAVNGKVEELVSGLKK"
     gene            19958..20173
                     /locus_tag="PLF77_02820"
     CDS             19958..20173
                     /locus_tag="PLF77_02820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07817.1"
                     /translation="MAIIRKELKEKSKNVLLRVSIPAPLMAEIKKIKKLCRDTGFYFD
                     IKPDVISAVEQAVEEAKALIQKEKKSR"
     gene            20207..21085
                     /locus_tag="PLF77_02825"
     CDS             20207..21085
                     /locus_tag="PLF77_02825"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HPC07818.1"
                     /translation="MTSTTMENLKKGKKALVVEGGGMRGVFAAGVLNAFGSGGFDPFD
                     MYLGVSAGACNLASHLAGQNDRNYDIIKRYSIDGRFINLGRFLRGGHLMDLDWLWDVT
                     ISEYRLDLKKLFGRLKEREKEYIIAVTSVATGKSAYLEPDEDTLEHYLKVSSSLPIFY
                     RNFLEVHSEKMTDGGVTDSLPVQEAIRRRATQITVIRTRPSQYIKKKSMFSMCYPIIF
                     RDYPLFAQAMKKRPGNYMESVRLIRHPPAGVRVFEIAPPPDIDVGRMTTDIKRLRAAY
                     QSGRDHGRKFMDAHAP"
     gene            21396..23627
                     /locus_tag="PLF77_02830"
     CDS             21396..23627
                     /locus_tag="PLF77_02830"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HPC07819.1"
                     /translation="MEQKHLAPFTPELTQNALTVLERRYLKRDKSGKILETPSEMFYR
                     VADTIAAADKKFNKKADTRKRAEEFYAMMASLEFLPNSPTLMNAGRELGQLSACFVLP
                     VGDSMDEIFDAVKFTALIHKSGGGTGFAFSRLRPANDVVLTTTGISSGPISFMRVFDV
                     ATETIKQGGTRRGANMGILRVDHPDIMDFIMCKADKKQLNNFNISVGLTEAFMTAVER
                     DENYDLINPRDKQVTGHLNARKVFNRIINQAWENGEPGIVFLDRLNRDNPTPQAGEIE
                     STNPCGEQPLLPYESCNLGSINLAKMVKDGKIDWARLKEIVRLAVHFLDNVVEVNKYP
                     LPQIAQMTMANRKIGLGVMGWADMLIKLGIPYNTDEAMELAEKVMKFINEQGHGASQQ
                     LARKRGPFPNFKGSIYDKKGQPPLRNATVTTIAPTGTISIIANASSGVEPLFAVSYVR
                     QVMDNDILVEVHPYFEALAKERGFYSPELMQRIAEHGTIHDLEEIPEDIRQYFVTAHE
                     ISPDIHIRMQAAFQKYTDNAVSKTVNFKKEATVQDVARVYELAYKLDCKGVTIYRDGS
                     RDQQVLSKGKKEETAVQEDADREQHLTKRERPKILKGWTYQMQTGCGPLYITINEDDK
                     GLFELFTTMGKSGGCAASQSEAIGRMVSLAWRSGLSSRQVIKQLQGISCHSPSGFGEN
                     KILSCADAVARAIQSHIEANGGKVQHEKIVFRKGACPDCGGIVEHEGGCAVCRMCGYS
                     ECA"
     gene            complement(23700..25787)
                     /locus_tag="PLF77_02835"
     CDS             complement(23700..25787)
                     /locus_tag="PLF77_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPC07820.1"
                     /translation="MEKFFYPRSMVVIGASVKRLNLGHIIVLNNRQHNYEGNIYGVGR
                     EEGDIAGAPVYDSVEKIPEVPDVAIIIAPAETVPDFMDACGKKGIKRLVIESGGFSEY
                     SQGQNVLEKKVLEIADRYGMRFIGPNCIGTINFDIKMMMPFGFFPRTAPGGRVAMIVQ
                     SGGVGGSYLGEFGDNAIIPGKFAAIGNKLQIDEVDVLEYLLNDDKTDVVAMYLEGFKR
                     GRAFFDLAVQSDKPLIVLKSNRSDLTAKIAQSHTTALSADDQVVDGAFRQTAVIRVDD
                     DIDFINAVKIIRLPLMKSRRVAILSRSGGHAVMSVDACAKFGFSLVDFPPAFIENLKT
                     IYNTRVIAHQNPLDLGEIFDYTIFTQILEEALKLPDVDGVLFNHLYIADYEGKMSRDF
                     LSNVGKLVQQYQKPVAMTMVTDTQELVNISKNQSYPIFTTPLTAAKALNMSATYYEEK
                     TAREARGRAVQTTLDMAAIDIIQDKCKTQKRIPLTDESLRIVEAAGIKSIAGATVKSA
                     KEAGDVSLHFPVAVKLLSRDASHKSDIGGVRLNIKNQRELKKAISDIKKSLKEMKPRP
                     AMDGFLIQEMSADGVECFVGGRQDPVFGPIVIAGLGGIFLEIFRDTSIRLAPVTKSEA
                     LDMLKNLQAYPILQGARGKMKADIDAFADVICRVSNLLAAVPDIAEIDLNPVIVHEQG
                     KGVSIVDSRIFFK"
     gene            complement(25836..26225)
                     /locus_tag="PLF77_02840"
     CDS             complement(25836..26225)
                     /locus_tag="PLF77_02840"
                     /inference="COORDINATES: protein motif:HMM:NF020839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPC07821.1"
                     /translation="MQDTQETFTSIGEIAKKLDMSQRTIRYYEEIGLLNSIKRVEGGR
                     RIYTEADFRRLKLIKRLKIMGMTLSEMQELEAMWTIEKSNDKVLGRLLELLNNQLRRL
                     DDRIADLNILRNEIVEYQKRIKSKIGK"
     gene            complement(26241..27797)
                     /locus_tag="PLF77_02845"
     CDS             complement(26241..27797)
                     /locus_tag="PLF77_02845"
                     /inference="COORDINATES: protein motif:HMM:NF006182.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-CoA synthetase"
                     /protein_id="HPC07822.1"
                     /translation="MSNIINRDTIGDMARRAATKYGDKTAIIFRNIKLSFYDLEREAR
                     RFANLMTRYGVRKGDRVAVYAYNSHYYPISMMGLAKIGAIQVPINYMLNAEEIAFIIN
                     HSGSRLFIIEDTLYPVIAGCKDKFPAVEKWGFIPLNDSGIPDGFFNLIAEMSAMNYDE
                     PQVEVSAEDIVQIPYTSGTEAKPKGAMLSHRALMSQYHSCIFDGRYETDDVSLHALPL
                     FHCAQLHCFLMPYLYVGATNVIMHKADPVEMIRLIEKYQITHMFAPPTVWIAILNHPE
                     FNNYNHGSLKKAAYGASIMPVEIIKQLSVTFKGLKLWNYYGQTEMGPVATILKPEDQL
                     LKPGSAGKPVLNVETRLMDDDGKFVALGDVGEIVHRSGHVMSGYLNDPEKTAEAFQFG
                     WFHSGDLGRFDNDGYLYVVDRKKDMIKTGGENVASREVEEVLYQYPGIEEVAVIGLPD
                     PKWIEVVAVVAVLKKGVSIQEKDIISFCKDRLAGFKCPKKVFITDRLPKNPSGKILKR
                     ELKEQLSSSI"
     gene            complement(27824..29464)
                     /locus_tag="PLF77_02850"
     CDS             complement(27824..29464)
                     /locus_tag="PLF77_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPC07823.1"
                     /translation="MSNFVKKTIGQLVRETAECYPQNPALVCPDFGIRQTYSEFYQTC
                     RDVAKGLIALGIKRGDNVAVWTTNIPDWIHLQFALGMTGGILVTVNTNYQTHELEYIL
                     KQSDSTTLFLMESYREVNFYENVRKILPELDHHQPGTPVSAQLPALKNIIYIGKREST
                     PGMLHFSDIITMGREVTDKQLEDRLHSLHDDDVINMQYTSGTTGFPKGVMLTHHNIVN
                     NARMVGDVMGMTEKDKLLIQVPLFHCFGCVMSSLNCVYHGSTMVVVEYFDPLKAVQWI
                     SAEHCTAVNGVPTMFIAILNHPDFDKYDMSSLRTGIMAGAPCPVETMNQVRTKMHCPE
                     IVIAFGQTESAPVMTMTRRDDPVELRVSTVGRLLPDIEGKIVDPDLGIDLPPNAQGEI
                     VTRSECVMKGYYKMPDATADAIDRYNWLHTGDLGEMDDNGYFKVTGRIKDMIIRGGEN
                     IYPRELEEFLFTHPKVVNVQVIGIPDKKYGEQVLAAIQLKAGQTATPEEFTEFCQGKI
                     ARHKIPKYWEFVDDYPTTASGKIQKFKMKEVFEKKYPA"
     gene            29934..30650
                     /locus_tag="PLF77_02855"
     CDS             29934..30650
                     /locus_tag="PLF77_02855"
                     /inference="COORDINATES: protein motif:HMM:NF016459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC07824.1"
                     /translation="MIPKIIHQTWKSTEIPPRWQRFRDAWKKFHPGWEFRLWTDEDNR
                     RLVERDYTAFLQLYDSYAYPVQKADFARILYLHKFGGVYADLDLEPLRPLDGIIDQPH
                     LILGEEKEGMGKSLNRCDFIINAFMASPKGHPFWEMLLERLRVTFRHKRLLQSKGHYI
                     IQTVIFELDKLARDYQQTHQDITIYPYEVFYPLSWHEACSELERSKAVEMSYTIHHFD
                     TTWFSGGMMLMKKLRRRLNI"
     gene            30732..32000
                     /locus_tag="PLF77_02860"
     CDS             30732..32000
                     /locus_tag="PLF77_02860"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPC07825.1"
                     /translation="MEKTLLIIFLILFVGRILWRFILQQLNIRHLRQCGKIIPPAFRN
                     VIDQATLSKMADYTYDNLRLENKENSTEDLLELAIIFLFLPLLVAKTNGLQLHLIWQA
                     LVFFGTLALIGGVIGLPFDIYRTFVLEKKYGFSTITWKLWLTDLVKSVIISAILMGFL
                     LSVFMAFVLYLPTSWWFWAWIFFAAFEILLLWLFPVVIAPLFNKYEPVKDEELKEKIT
                     SLLSKVGLKAKGIFQVDEGKRSKHTNAYFTGIGKTKRIVLFDTLLASHTHDEILAVLA
                     HEIGHWKKKHILKQLIFMVAASFVGFYLVYLLVNWPPIYGAFGLPQTPVYAGLLLVSL
                     YFSCVGFFFSPLAAFISRRYEREADRMAHELTGTSEPMIHALKRLAKDNLSNLHPHPW
                     YVWFYYSHPPLIERIEYLKTMDKGNLQRQR"
     gene            complement(32007..32633)
                     /gene="thiE"
                     /locus_tag="PLF77_02865"
     CDS             complement(32007..32633)
                     /gene="thiE"
                     /locus_tag="PLF77_02865"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPC07826.1"
                     /translation="MRTFLRHGLYLILTDPRDGYETLCRWAVEAGLPAVQLRYKGHNE
                     REHLALARALRQITKGTGTLFIVNDRPDIALISQADGVHLGQDDLPAAEVRQLIGPTM
                     LLGLSTHNLEQVAAANAVPVDYIGFGPLFATNSKERLAPVTGPDILKAALAISRYPIV
                     AIGGLTLERIRQLQPRPHNTAVIRAVNDASDPLAVMRAIHESCMNRTS"
     gene            complement(32654..33424)
                     /locus_tag="PLF77_02870"
     CDS             complement(32654..33424)
                     /locus_tag="PLF77_02870"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR00292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HPC07827.1"
                     /translation="MLNEIIISRAILDSYFKKLGSCLDLDVAIVGGGPSGLVAGYYLA
                     RAGRKVALFERKLSIGGGIWGGGMMFNSIVVQEAGRQLLEEFDLQGREYTPGYYVLDA
                     VDITSTLIHKAVRAGLQVFNLIAIEDVVIKNERVAGLVINWGAVDTLKWHVDPLTVHA
                     RYVIDGTGHPANVTEVLVRKMGVRLNTSTGGIVGEKSMEAGKGELQTVENTREVYPGL
                     FVSGMAANAVYGGYRMGPVFGGMLLSGLKVAEEILARL"
     regulatory      complement(33506..33598)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(33652..34287)
                     /locus_tag="PLF77_02875"
     CDS             complement(33652..34287)
                     /locus_tag="PLF77_02875"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPC07828.1"
                     /translation="MIKTIFWDNDGVLVDTEGLFFRANQEILAGLGIPLQREQFEEIS
                     LVRGESVMRLAATPGTDAFIKLRRDRDALYADLLTRDPLVIDGVREVLDALQGKYLMG
                     IVTSSGHEHFEIIHRQSSLLPYFQFFLTLKDCPYTKPHPGPYLKAIALSGFAPEECLV
                     IEDSPRGLAAATSAGLRCIVIPSKFTCNHDFAGACKVLDSIKGLPAFLKEF"
     gene            complement(34546..35448)
                     /locus_tag="PLF77_02880"
     CDS             complement(34546..35448)
                     /locus_tag="PLF77_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HPC07829.1"
                     /translation="MNIGELLGAMVQTGMSPSSSARMKNSLGGGKELDSLKDMLGDAA
                     GQSGSGGLGGLLSSVLGGDKSSGGKSGGIGDLLSGVLGGGQKGGASSSGGIGDLLSNM
                     LGDAGKAVGGNKNLAIGGLGALIGSIMGGGGKSAGGAVGGGLMALLGALAYNALKGIG
                     QAKTPIPAGLIEPGTDEEQKQLDQSSELVLRAMINATKADGKIDQNEINRIMGKFNEI
                     GVEEEGKKYLMMQLQQPMETDTLIAAAQGQPDLAAQMYAASLMAIEVDTQNEKDYLEK
                     LAEGLGLGSQVTTRIEQMVGLQPS"
     gene            35838..36986
                     /locus_tag="PLF77_02885"
     CDS             35838..36986
                     /locus_tag="PLF77_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HPC07830.1"
                     /translation="MSPKSKEEYFEVLYKRYKEASRKEKTVIIDECCAVCGHHRKHAI
                     RRLKGYKRFTKPKAKKRGKPSVYNKAPIIRPLKQIWLAANLPCSKRLKAILPLWLPKY
                     SEHFGGLAEDIIRTLWAISPSTIDRILKPIRIQYQKRGKSTTKPGTLLRKQIPINTNQ
                     WDESRPGYLEADTVAHCGDSLMGMFAYTIDFVDIATGWTEQRSVWGKGEKGVLEQIKD
                     VEKMLPFPLLGFDSDNGSEFLNHHLLRHFTDRKQPINFTRSRAYHKDDNAHIEQKNWT
                     HVRQWLGYQRLDNPKVVPLMNNLYRNEWRLFHNFFLPSVKLIEKERIGSKTIKKHDQP
                     KTPYQRIMESKYIDQSVKLALSKQLENLNPFELNNLIQMKLKKIARLR"
     gene            complement(37358..37900)
                     /locus_tag="PLF77_02890"
     CDS             complement(37358..37900)
                     /locus_tag="PLF77_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07831.1"
                     /translation="MPANEPQKLPKTWTTEMLNAFHKLINSPLPVSFTVSEKNKLTTF
                     FGKQNPSSHARVACHVSYKGNISVPEFENVGVVFNPAKNLTDEYKNFIGYIEIVNGLK
                     EDTIPVPEIRVFIQEDSGVCDVFSEMLIESKTLHAKLSPIYIYLEQSYCLKDKPPEDL
                     LKKRIPIKEISTFQKISCVE"
     gene            complement(38236..39069)
                     /locus_tag="PLF77_02895"
     CDS             complement(38236..39069)
                     /locus_tag="PLF77_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPC07832.1"
                     /translation="MRLERETKEFFEKYRQRYLDNRQRFSDGLNKTLAEKKDWIAHIS
                     YQPYKWLFVLPLLIATTLICGVLAIVTALLFSPRTGGIFGMIWARINSFCTPMTVHVV
                     GADNIEPGTSYVITSNHQSMYDIYVLYGWLGIDFKWVMKKELKKVPVLGLACKIIGHI
                     YIDRSDSKSAVDTIKAARAKIVGGTSVLFFPEGTRSVDGKMKQFKKGAFKMAIDLGIP
                     ILPVTINGTRNILPKGSLDLTPGGATMIIHKPIPVTDYHDGNLPELMEHTRRRMNAFL
                     D"
     gene            complement(39107..39595)
                     /locus_tag="PLF77_02900"
     CDS             complement(39107..39595)
                     /locus_tag="PLF77_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07833.1"
                     /translation="MAQKITISVPDELYEKMQAWKSSLNFSRVFQNAVSKIIQKKENL
                     TSKIRNEIEFSSIVDRLKKEKVDYELNIAEWGKKDGLEWCKTAHYREIQYALAWTACE
                     NPNQDEELGDYFSDIFKKYKAQVAATGKKAQEAWSDFSEKYLKGWREGVELFWHEVKD
                     KL"
     gene            complement(39955..41856)
                     /locus_tag="PLF77_02905"
     CDS             complement(39955..41856)
                     /locus_tag="PLF77_02905"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HPC07834.1"
                     /translation="MADKSKYAGVYSESINQPDIFWGKAAEDVVWNKKWDKVLDDSKK
                     PFYRWFSGGELNTCYNALDYQVESGRGEQNAIIYDSPVTNTVQKITYKELLDKVSKFA
                     GAISALGVKKGDTVIIYMPMIPEALVAVLSCARIGAVHSVVFGGFAPNELAIRIDDAK
                     PKIIISASCGIEGKKTIPYKPLLDEAIKIAAHKPQKCVIYQRPQVKADMDSARDLDFN
                     DVMKNAKAAGCVSVAATDPLYILYTSGTTGKPKGVMRDNGGHAVALKWSMKNIYDVKP
                     GDVFWAASDVGWVVGHSYIIYAPLLYGCTTIVYEGKPVGTPDPGAFWRVISQHGVSVL
                     FTAPTAFRAIKKEDPNGDYLKKYDIKCLRYLFLAGERLDPDTYHWASDMLKIPVVDHW
                     WQTETGWPIAANCMGIERFPIKAGSPTKPVPGYNVQILDGDGNQLPNGEEGSVVIKLP
                     LPPGCLPTLWNDDKRYLDYVMERPGYYVTGDGGRFDQDGYIFIMGRIDDVINVAGHRL
                     STGAMEEIISKHKDVAECAVFGAEDQLKGQVPVGFVVLKAGVDRDPDDIVRELVQMVR
                     SELGALACFKEAAIVKALPKTRSGKTLRSTMRKIVDGKEYVIPSTIDDPSVLGSIAEA
                     AKTIGYGKK"
     gene            complement(42138..43301)
                     /locus_tag="PLF77_02910"
     CDS             complement(42138..43301)
                     /locus_tag="PLF77_02910"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPC07835.1"
                     /translation="MKLDDIKKIAVIGSGAMGNGIAQVCAQAGIKAVMVDIKQEFLDK
                     GMATVNKSLDTLVGKGKMTAEKKAEVMGNLSTSLSNTEAVKDVQVIIEAVPEIMSLKK
                     TVCAEISAAAPADALIASNTSTMSISEIATAGKNPERILGMHFFNPAPVMKGVEVIYA
                     KQTSDANVDLLCELTKKIGKVPIKVLKDSPGFIVNRIGAPNQALISAILDEGKIKVDT
                     IDAVMKAKAGMPMGPFELADYVGIDVFYHTLKYYSETLSPEFKPGKTLQTLLDSGKLG
                     MKSGQGIYTWEGGKAKIDMSVESQDFGPLDFLAIQINEAVRVYKEKIAASAADIDLGM
                     VHCMRAFAGPFAFGAGMEPAQLADTLNKLHTRFGLNIFKPEPEIVDGSFKNMK"
     gene            43689..43985
                     /locus_tag="PLF77_02915"
     CDS             43689..43985
                     /locus_tag="PLF77_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07836.1"
                     /translation="MNSLKKAIFIVCFFNLLGLWSLSLSGVAIANQNEQEASLEKCIK
                     SCEDSKQVCWNMDPDRRLCEAQFQLCMENCNKKYNAPVVEDVKPPEPKKTRTLH"
BASE COUNT        11040 a        10671 c        10819 g        11690 t
ORIGIN      
        1 atatcggcac aagccctctt tacgccatca aagcctgttt ttacggacag cacgcccttg
       61 ctcccgacat aacgaatatt ctgggcgttt tgtcgcttat cttctggtcg atgttcgtaa
      121 tcgtcagcat caagtatgtc attttccttc ttcgtgccga taatcgcggc gagggcggca
      181 tttttgcgct ggtcagtctt ttgaatgtgt cgaagaaaag tttttctccc cttgttcagt
      241 ccgttcttgt gcttgccggg attctgggcg ccgggctttt gtacggggat ggcgtcatta
      301 cgccggccat atcggttctt tccgcaattg aaggattgga ggttgctacc catgctgctg
      361 cgccttttat attgccgttg acctgtgtcg tgctcattct gctttttgta ttacaacgca
      421 aagggacgtc gaatattgga aaggtgtttg ctccgatcat ggttttatgg tttctgtcca
      481 ttggtatttt cggcatcatg cagatcgtac gcgaaccttc cgtccttcgg gcgttgaatc
      541 cgctatatgc cgtggagttt tttctgaaca acggaatgca cggcgttgtg gtgctgggat
      601 cggtggtgct ttgtattacc ggttgcgagg cgctttacgc tgatctgggg cattttggcc
      661 gaaacgccat tcgcctgtcg tggttttcac ttgtttttcc ttcattgctt tgcaactatt
      721 tcgggcaggg agctctgcta ctcgcgcatc ccgaaatgaa tgtcaaccct ttttataaaa
      781 tagtgccgga aattctgctc tacccgatga tcggactttc cacggccgcc acggtcatcg
      841 cttcccaggc gttaatatcc ggcgcgtttt cacttacgca acaggccgcg caactgggat
      901 tatgtccccg ggtgcggatt attcacactt ccagcgatat gcagggacag gtatatatcc
      961 cctctgtcaa ctatgccctg atgatggcgt gtattgcggt ggtgacgggg ttccgggaat
     1021 ccggcgctct ggccggcgca tacggcatcg ctgtgacggg cactatgatc atcacctcaa
     1081 ttctttattt tctggtgctg acccatcaca gaggatggcc cctgcggaaa gtcattcccc
     1141 tcgtcggact gtttctttta gtcgatatcg cttttttcac gggaaacttt ttcaagctcg
     1201 ctgatggagg gtggttgcct ctcttgatcg cagcaatcat tgcggtagcg atgaccacct
     1261 ggaaaaaagg acggaatgaa ctttaccgta atttggtcgg agcaaggttc ccgctggaaa
     1321 gttttctttc tgaattgccg aagcgccata tgcccagagt gccgggaacc gctgttttta
     1381 tgacgctgtc tcccgtggga acgcctccga cgctgcttca taatgtaaaa cataatcaca
     1441 tcctgcatga aacagtagtg cttctttcca ttatcaccaa agatacaccg gccgttcctg
     1501 ccggtgaacg cattaagcta gccgatctgg gtcaggggtt ttaccgggtg gaggcatttt
     1561 acggattcat gcagaaaccc aatgtgccgc agttgatgaa aatacttgcc cagtatgggc
     1621 ttgttacaga cccgatgacg acgacatttt tcctgggcaa ggagactctg cttacgggag
     1681 gaaaatcgaa aatgatgaaa tggcgcaaat cattatttgc ctttatgtcc agaaatgcgg
     1741 ggaacccgac gtcttatttc ggaattcccg ccaatcgggt ggttgaactc ggcacccaga
     1801 tagaattgtg atgttccaca gcggaatcat aaaaagggct gaaggtgatt ttcggcggaa
     1861 tcagaaaata atatttcaga agacagtaaa ttcaaagaag gtttgtgagc tgaataatga
     1921 atttcttctt actttacgat cttgaagaat tctttggcct tcggatcggc ggcggtgcgc
     1981 agcagttcaa ataagaccat ctccgtgctg gcgatatgag ctccggcgct tttcatcgct
     2041 ttaatcccga tctgacggtt ctccggcgtg cgcgatgaaa cggcatcggc caccagatgc
     2101 acgttgtatc cttcggacag gagatccagc gccgtctgat aaacgcaaac atgcgtttca
     2161 atgcccagca gcacgacgtg cctgcggctt aagctttcca gtcgttcttt aaaatttttt
     2221 tcaccccagc agctgaagga ttcctttgcg atcggattga agtcttcaat ttcgtccgct
     2281 atttgcggaa gtgttttacc cagtttcccc ggaatttgct ccgtgacgat tatcgggaga
     2341 ttcaaggtct tgaatgcttt aatcaacttg acggcattgg caaaaatatt ttccttgcca
     2401 aacatggcct gagccagatt gccctgaata tcaatgatta ccagaaccgc tgccgttcga
     2461 ctgatcatcg agctttctcc tgtcaggacg ggtgcatgcc gagcagaaca ccgccgtcaa
     2521 ttacaatttc cgctccggta gtgtagctgg aggcatcgga tgccaggtaa agcgcggcgc
     2581 cggctatttc atcgggatcg ccgatgcggc cggagggcag cgctttttcc accagttctt
     2641 tcactttttg agcgtcttca gggggaagaa attcccagtt ggatttcaac atcttggtgg
     2701 cgatgggtcc cggagcgatg gtgttgacct gaatgttgta ccggacaagt tcgctggcaa
     2761 aagccttggt cagcattcga atgccggctt tggagatagc gtagatgctc agacccggtt
     2821 ccggattgtg accgtcgatg gaggcgatat tgatgatctt gccgccgccc tgcttgacca
     2881 tgatattggc cgctgcctgg ctgataaaat acgcgccttt gaggttgata tccatcagct
     2941 tgtcccagag gcgttcgtcg cactccagga cggaaccttt cgcgggactg gcgccggcgt
     3001 tgttgaccag aatatcaatc cggctgtatt tggccatgac ggcgtcaatc atctttttta
     3061 tttcttccga tttgcccaga tgagccggca gcgggtaagc gtccccgccg aaagctttga
     3121 tttcgttggc tgtcgcttcc agatccggcg ccttgcggct ggtcacaacc accttggctc
     3181 cggccctggc aaaagcgaac gcgatagcct gaccgatgcc gcggcctccg ccggtaatca
     3241 ccgccacttt atcttttaat gaaaattggg atacgtcgag catattttat tctccttgag
     3301 aaatctaacg cctcctctgt tgttggcgac ggggcaaatg atgtctatta caatattgtt
     3361 tctgtttcca gtgaaattat caaacttctc tggctcaacc gccgatgccc gccagacgtt
     3421 taatgagttt ctttctgtcc ttgatatcat actgggacgt aatggcaatc tgctccatga
     3481 tgggcgatcc gccgccgtga tagttcccgt agttcatgac accggccatg gtgccgatgg
     3541 tatagtcgcc cagcgtcatc caaaacttga tctggtcttc cacagggacc ttgggattcc
     3601 ggttgagata cttttccaga actttcttca ggtcgggatg cgtgaggtcg ccttcatagg
     3661 ggaaggtgga cggcaggccg ccggcgatcc ggcagatcag ttcctgttca tggaaaaccg
     3721 cttctcccgt taggcaccgg cccacgttgc agtaaatgga attggggatg tagctgcctg
     3781 gtccgtaagg gcacataccc aggccgggga taatgacttc gggcttgccc atgctggaag
     3841 ccgtataccc tgcggcataa cccagctcgg acactttgat gatctccgcc agcatctctt
     3901 tcacgtgagg cgttttctcg atgccgttgt attccgccgc caaagctgcc aggccgagaa
     3961 aaacatcccc aatggcgggc ttgcatcccg aatagctgtg gcggtggaac agcgcgaaca
     4021 gcagggcgca cattccgccg tgatcgtttt cgccgcagag gaagacccgc tcccagggaa
     4081 caaaacagtc ctcgaaaatg atgtaggaat cggaataacc gatgtctatg ccgcgtttga
     4141 tatgtttgcg cttgtgctgg ttgtgaatgg aaacgacctg cttgacccct tcgtaatcgg
     4201 agggaacggc aaaagccacg gcgtaatctt tttcatcggg acccagggcg cgggtgggca
     4261 ccacaatgat ttcatcggag attgacgcct cggagatgtg gagtttgcag ccccggacga
     4321 caatgccgtc ttttttcttc tcgacgacgt ggacgtacat atccgggtcc gtctgttccg
     4381 cgggtcgttt caggcgctcg cccttcatgt cggtctgggc gcagcttccc acaaggtctt
     4441 ccttctgaaa acgttcgagc catttaagga aattcttgtg gtattgtgta ttgccgttgt
     4501 tgaccttatc ggcctcaaac gaagcggcgt tgacggcatt cagggcatcg gtccccatgc
     4561 atcgttgaat acacatccct gccttccggc ataaagcccg ggtcatgtcc tgctttttgt
     4621 gaagatcgtc tgtgctttga tggacatggc aaaagcgatt gatcgtttct cccgttaaat
     4681 gggaagtcgc ggtgcacaga tcttttaact ccggatcatg aacggcgtcg aaagtcagtc
     4741 ccatcacatt gagcacggga gcctggatct catcatcacg gtcgatcttt tcgccgtcat
     4801 ggtagaggtt ccttctcatt ttccggagcc tttcaatgta ttgttctctc gttctcatgg
     4861 ccgcacctct tctttgtttg ctaaaaatgt tatcttattt tttcttttga tgacgtttct
     4921 ctgcccttct tcagaatttt ttccaggacg ccggcatcgt ccagtttcag gtaaagcgtt
     4981 gttccggaat cctctttcct cgcgcggatg ccgtcaaaaa cggtttcgag cagggcgtca
     5041 agcatctccg cgatgcccgg tttttttttg ggcatgccct gcatgtgcca gtgaatgaat
     5101 aaatgctcga tcgtacccat aaacacggag cgaatcaggt agggattgga atcctttttg
     5161 aatgtgccgt cggcgattcc ttcccggatg cagtccagca gggtatgcgc cgatgtgcgt
     5221 attgcggcat gggcgggtgt ctgtcgaaag cgcctgttgg acatcagttg caggagaacc
     5281 agtgcggaat agttgggatt ggattgataa agcttgacat agaaaagtaa aatcgccctg
     5341 attttgccct ccacgccttt gatgtaggga atgacctttg aagattcctc aaatgtgtcg
     5401 ttggttattt tttcaggaat cgcaaagaga aggtcttcct tagtgccgaa gtattcgtag
     5461 attgtcgctt ccgaaacacc ggcggctttg ctgatttcgg tgatggtggc gttctgaaaa
     5521 cccttctcgg caaagatgcg cagagcggca tccataatgc ggtcttttcg ggtcttgggt
     5581 ctgttcttgt ctgtcatgat catttcctcc cgcgagagtg attttaagat acatattcat
     5641 agtcgcactg tcaagaatta ttttatttca gagcgtggct atgaaataaa atagaaatat
     5701 catagtatgt cacgatgata tccattgata aaaacgggaa ggatcggaat aatgcttaag
     5761 ggaattagat aattccacga atgtatgatg aaggcttcac aaggagatga acggctttga
     5821 gagatttaag gcctgatttt acgcattgtt ctgttgagtc tttttgactc aaccatcctc
     5881 aaggatatcc gaattagaat attttatagc ttcaatttgt ttagaacaat ttcagccaaa
     5941 tccttttccg gcgcatcgcc gtaatcaacc gaattgccgg caatgataat ccatctgcgg
     6001 gcaagattcc tgaaatccgg cttatccgcg caggcgctca gacagccaca aacgagaata
     6061 ccgatgtccc attgagttgg agattgatcc gttgcaagag tgaactcagc cgacagccgt
     6121 ctttggatgt cctgaacgag ttgtgctcta ttgatgacgg gattgcaccc gccgcagtat
     6181 ttaaatccaa tcaactttct ttctgatatt ttgcagttca tcaaggcaag cccggtcaat
     6241 ccttgatgcc ggctatttct ttggccagtt tctttttgtg ttccagattg ccctggcggg
     6301 caatcatgat gcgctgagcc tgcggagaac cggcgccgtg catggattct gttcggtagc
     6361 cgacggcggc agtgcccagt gtgatatttt ccaccagccg cataattcgg catcggtctt
     6421 cagtcggcac ggaagcgatt cctttgaaat atttttcgac tattttgccg atttccggat
     6481 gacgcagatc cttttccgag gccatggtca ccatcaatcc tccggtgata tcttccgcga
     6541 gtcgggcgat ttcgtaggga aatctggtca cgttttgctt gcagacattg gccagcagca
     6601 gatcgatcag gtacgttccg gaagcggtcc tgtgcccctg cgcagagcag gcgatgccgc
     6661 aggagaaaag ggtttcgttc aaatgtgtca tttcaatgag cttgtctttg acgtgagagg
     6721 ctttggccgc gccgttgtaa tcggccgcca gtgccgccgc gccgatcagc acgtcgccca
     6781 cgccgacctt gcagccgccg tagctctgac gatggtagcc ggcaaagcgc tccaccagga
     6841 gtccgctgaa ttcatattcc ccgcacatga agacattttc ccagggaacg aataaattgt
     6901 caaaaaccat caaggcttca tgaccgccga attgccggtt gccgacatct atttcacagc
     6961 cttcgagttt gcgcgtgtcg caggattgcc ggccgtagat gtaggaaatg ccgtccgcgt
     7021 ctgcgggcgc gacaaaggaa agcgcgtaat cggcgtcgtc tttggtcatg gcgatggtcg
     7081 gcatcaccag aatccagtga gagttgacgg ctccggtctg gtgggccttg gcgccgcgca
     7141 cgataatgcc gtcttttctt ttttccacaa catgggtgta caaatcagga tcggtctgac
     7201 ggctgggcgg cagactgcgg tcgcctttcg gatcggtcat ggcgccgtcg acaacaagat
     7261 cttcttcctg caccatttta agaaacctga taaatcgatc atggtattgc gtgccgagtt
     7321 tcttatccat ttcgaacgtg acgctgtcca cggcattgat ggcatccatg ccgacacagc
     7381 gctggaaaca ggcgccggtt ttttgtccca gcagccgctg catttttact tttttgatca
     7441 ggtcttccgt gctttgatgc agatggcaga aacggtttat cttttctccg gtcagatgag
     7501 atttcgccgt catcagatct tcatgctcgg ggctttgagc cagggcgtac gtcattttta
     7561 cggaattcat ggaaggcctg atgattggat gatcggcggg gcatttcacc tgttcaccca
     7621 aaaggtagac tttcagattc attttacgca ggctttcttc gtattgatca gcggttttca
     7681 atcccatttc agacctcctc gggcatgaac aaattttgtt ttgaaaaatt cacaggacaa
     7741 ttttcattaa gttaatactt tgttagataa atttcaaggc cattcggatg cgtattatat
     7801 catgttccgc caaagtgata atgtcctatt ccaccaaagt agaaatgtcc taagataaag
     7861 ccgtaattgt ttcctgcaag gggggaggta attatggctg aaagggacat ggtggtcatg
     7921 agtttaggag aattaaagcg tctgaagttg atccagtcgg cgatagacag acaaatcagt
     7981 cagaagcaag tatccggcat attaagactc agtgagcgtc agatcaggcg gttagtgagg
     8041 gccgttcgcg aggaaggcga taagggtatt ataaatagat cccgtggtcg gccatcaaac
     8101 cgcggattat ccgctgggac aaaagagaac gtcatcgctc tatacaagga gaagtattct
     8161 gattttggac ccacttttgc gacggagaag ctatgggagc gagatggtat ccgattaagc
     8221 gatgagacac tgcggaattg gctgataggg gccggtttgt ggaagaagca tcgcaagcga
     8281 tcagcataca ggcaatggcg tccaaggcga tcctgttttg gagaaatggt tcaaatggac
     8341 gggtcccatc atgactggtt ggaaggtcga ggtcccaagc tggtattaat gggctatatc
     8401 gatgatgcca cgaatacaat ctatgcaaaa ttttatgatt atgaagggac cttgccggcc
     8461 atggacagct tcaagggtta tgtcaagaag tacggtcttc ccatgagcgt ttatcttgat
     8521 cgtcacacga cctacaagtc ttccaggaaa cctacggaat gggatgaagc aagtgatcta
     8581 aagtttctca gtcagtttga gagggtcctt aaagagcttg gcgtggaagt cattcatgct
     8641 ttgtctccgc aggcgaaagg aagggtggag cggctctttg gagtccttca ggatcggctg
     8701 gtcaaggaga tgaggctcca cggcatcaag acaaaagatg aagccaatgg gtttctggaa
     8761 acgtacttac cccaatataa tgaaaaattc agagtgtgtc cggccaatga gacggatgtt
     8821 catgtcagac ctcctcgtca ttttaactgg gacaaatatc tctgtatcaa agaccaaagg
     8881 acgatccgta atgatcacac cattgcctac aacagaaagc tttatcaatt agaggagaac
     8941 gatagtaaaa aggtccatat tgaaaagaga agagatggtt ctcttctcat catcagcaag
     9001 ggcagagctt taaaatacaa agaaataacc gaaagaccca aacagatggt ggctgttaag
     9061 aaagacctga gagcatataa ccggccccct aaaccatcaa aagatcatcc atggaagcaa
     9121 aggtggaaaa cctgggggac aacgccaact gttttatgag aacgatattg atatctatat
     9181 tttttcttct aataggacat ttctattttg gtaaaaatag gacatttcta aattggctgg
     9241 acaacgcgta ttatatcctt gacatacgat gggggacttc ttatactcca acgactcgcg
     9301 attgggttat tccggttgcg ggctgggctt atgctgttga aatagtttaa tattatagcc
     9361 tttgcggccg taatttttac attttgaaaa agagaggtaa aacatatgtc tatcatgaac
     9421 tggaccaaag atgaaaccgt cgccattata accatgacca atggtgaaaa cagacaaaat
     9481 ctgacatttg ccaagacatt gaatgcgatg cttgacgaaa taatcgcgga taaaaccata
     9541 accgccctga ttattacttc taatgaccca aagaattttt cgcagggcat cgatgttgaa
     9601 tggatcatgg ggcagatgca gaaaaatgaa catcagccgg taacggattt catcaacgaa
     9661 atggatgtgg tttttaaaaa attgcttctt tatccgatcc cggtcattgc agccatcaac
     9721 ggacatgctt tcggcaatgg ttcgattatc gcctgcgcct gcgatttccg attcatgcgc
     9781 tccgacaaag gattcttctg ctttcccgaa gtcaacatca gcatcccctt ccgctggggg
     9841 atgaacgctt ttgttaaaaa ggccatcccg atccacttgt tgtcgcagat gcagttgacc
     9901 ggtaatcgtt tcaccgcgcc ggaactggag aagcacaaca ttattgtgaa ggcctgcgcc
     9961 aaccaggaag aattaatgtc ctccgcgata gcttttgcca agactttcca gaaaaaacgg
    10021 acaattttcg gcgaactgaa aaggaggctg tacgccgata tcatcaaagt tctggatgat
    10081 gactttatgc ctccgacttc caagattact tcgctggtga tgatggatta gcattgaaca
    10141 aaagttatga ccatttaaag aggtacaaaa aatgaaaaca gcgattaccg aaatgttcag
    10201 cgtcaaatat ccgattatct gcggagccat gatgtggctt tgcaggcccg tactgtgcgc
    10261 tgccatatcc aatgccggcg ggctgggcaa tctgacggcc ggaaattacc ccacggaaga
    10321 cgaattccgt gatgccattc ggcagacacg aaaactgacg gataaacctt ttatggtaaa
    10381 tgttactatt ctgccgtcag tccatgtaac agcggaacat cacaaaatgt atttaagagt
    10441 gtgtgctgaa gaaaaagtcg ccggtctgga gatatccgga accccgttgg ataaggcggt
    10501 gggcaaggaa tttatagaca ttctgaaaaa agccggtgtg aaattattcc acaaggtcgg
    10561 atcggtgcgc catgccgtgc acgccgagaa gaccggctac gacggcattt acgccgccgg
    10621 tattgaagaa ggcggacatc ctctcaatga cgatgtcacc accatggtgc tgacgccgcg
    10681 tattgccgaa accgtgaaaa ttccggtggt aacggtcggc ggtgtcgctg acggacgaac
    10741 ccttgccgcg gcactggctc tcggcgccca gggcgtgatg atggcgaccc gttttgttgc
    10801 caccaaagaa tgcgaagttc acgacaatat caagcaggaa attatccagc gtcaggaatt
    10861 cgaaacagcg ctgatctgca aatcgatcgg cctacagggc cgggccatca gaaacaaact
    10921 ggtggaggat gttctggccg tggaatcgac gggcggcgga ctggaaaaac tgattccgct
    10981 gattgccgga gagcgcgtca aaaaggcctg ggagtttgga gatgtggaca gcgctccaat
    11041 gatgatggga caatcggttg gactggtcaa ggatattccc acctgcaagg agcttatgga
    11101 aagactggtt gccgaggccc aagccgatat tgagcgcgtt agaaatattt tttaaaaata
    11161 tgtttgctca ttagaaatga tggatagaac tctgatgtcc gtttccggga aagtctgaat
    11221 gtctggtgtg acaacaaagg aaacagcgct cgctacaaag ctttcaaaac cggaaccgcg
    11281 ggatgaaacc gttccgggca gcaacgccca cggccgcttt gtgtcgcggg aaatcattgc
    11341 cctgatgggg aaagacgaca tcaccagcgt ttccctcggc gatcacattg aaaaaacaat
    11401 gaccatcctt tttgcggata tcagagggtt tacctctctt tccgaagaaa tgcccccccg
    11461 ggaaaatttc acgtttatta atgcctacat gaagtgcatg aatccggtga tcgcgcgtca
    11521 cggcggtatc atcaacaaat acatcggaga cgcgatcatg gcgcttttcc ccgccgatgc
    11581 cgacgacgcg gtgaaatgtg cgatcggtat gctcagacaa ctggtcgcct ttaaccgctc
    11641 gattaaaaaa acaggttctt cccgtatcaa gatcggcatc ggattgaata ccggcctgat
    11701 gatgctcggc attgtcggcg gcgagcattt tatggaaaca accgtcatca gcgacgcagt
    11761 caatctggcg tcccgcatcg agtccatgac gaaagattac aatgtccctt ttctgatcag
    11821 cgagcacacc tattacagtt tgttcggcgc atctgaacac aatacacgtt ttgtcgaccg
    11881 cgttcgggtg aaggggaagg cacaatgcca gtcggtttat gaagtctttg acgccgatcc
    11941 gaaatcttta cgcacggcta agcaaaaaac aaaagccgat tttgaagaag cgctggcctt
    12001 ttatcatttc aaagatgttc ccaaggcgac gaagctgctg gaaaagcttc tgcgacgggt
    12061 gcccggggat actgtggcaa aggtttatca tgaacgttgc ctccgttttt tgaaaacagg
    12121 cattcatgaa agcagcgggg aatttgacct ggctatttca tggaataaat cgatggcgat
    12181 agatcactcc ctcatcgatg agcagcaccg tgacctgttt attcacaccc gggagtttgt
    12241 ggaagcggtg aagacaagca aaaattattc gcagctccgc aatgtccttg agtttatgga
    12301 taaatacatt caggttcatt tccgaaccga agaacgttta atggcgcaaa aaaactatcc
    12361 ctttttgcag atgcaagtcg atcaacaccg gcgcttttcg aggtacttcg acggactgag
    12421 aaatgaaata aaaagaaatt ttgaaactaa caggattttt attcttttcc gcattcaggt
    12481 gctgatcgtt gactggctta ttaatcatac cagcaagctc gacctgcatt tcggcaaatt
    12541 cctgcgttac cggaaaggtc gtggactgaa gagcctgtct taagagcaga cggaaatcac
    12601 cgtcaatcag ataaacgcaa tttccgttgc ggcggaagag acaacaaaac aacgttcgtt
    12661 attggagttt gcatcggccg gaacgagaaa gaacccggga cggttgggct gatagccgtt
    12721 ggttgtgccg accattaatt ccccgtcttt aaaaatgacc tttatttttc gtcccgccaa
    12781 gggtcgggaa gggtcgaact ctttttgctc gtgataactt ggattgccgt cataatcttt
    12841 aacatagaat aaacctttta gaccgtccat cttcaatgtc aacggtctgt tgtcttcctg
    12901 agccgctctc tcaaacagat gaaaaagttc cttattgggg gaaaaatcgt tggtaatgcc
    12961 tttaaacaaa ttgccgtcct ggtaatgtgc cacgatttta ttttgagtca ttgttttctc
    13021 cttacgatta taaagggata gccgtcattt aaaactgccg tctgatttca ccggcgatca
    13081 cataccattt ttgcacgcca ttttcgagcc ggaaatagtg cagaagatag gcagcggata
    13141 gaatcagcag gataacaaaa agtagaataa cggaaaggga aggtctgata acggcgaatc
    13201 cgaaaatcat cattgtgaaa aggcaaacga agagcatcac aattaaatgg accaggcgtt
    13261 catgctgtat ccatttgatc tgtttgtcgt ggtaggcgag aagctccttt aatttttcgg
    13321 catcgggatt ttgctccaga atgcctttta cgtaattttc atgatttttc atgtaagaaa
    13381 tcattgccaa tcccgcaaat atttttatga atattaacat attttaatga aaatacagat
    13441 atctttttta agattgccgt aaattaatgc ggaagaatcg atacttataa aattcgtttg
    13501 ccacaatctc cctgtaatgt tatgtatccc ccgtaagggc agatcacaag gaggaaggaa
    13561 gatggtaaat aaggttatct tgattggaag attgggcaag gatccggagg ttcgctacac
    13621 gcctgatggg acgatggtta ccaacttcaa tttggccacc gacgagcaga gaaaagataa
    13681 aaacggcgaa aaaattcaaa aaacagaatg gcataaaatt gtaacatggg gaaaactggc
    13741 tgaaatctgc ggtaattatc tggtgaaagg gaagctcgtt tatgtggaag gtcgcattca
    13801 aacgcgttcc tgggaagaca aggaaggcgt caaacgttat acgacagaaa ttattgccag
    13861 tgatatgagg atgctcgatt ccaaaggaca aaccaaatcc ggagagactt cttttgatgc
    13921 tgatgccgct aatgtgaatg acggcagcgt gccgttggac gacgtgccct tttaatatgc
    13981 ggtaatttat gaaaaagaac cgtttggaaa gttaatgcac gatccttctc catcctatac
    14041 tttttaaatt ttctgcgcat atcttttcaa aaaaccgacg gggataatgt ttttttatgc
    14101 ttattgcccg ccataggaaa aattatgaaa aatatcaatg tgttgatgat acaaccgaag
    14161 acgccgcaaa ctttttggag ccatgaccat accattaagg ctcttggatt caaagccacc
    14221 atgccgccac tgggattgct gacggtagct gccatgcttc cccaaaactt caacgttaaa
    14281 ttagtggatc tcaatattga agatctggac atgcgggatg tggataaaag tgacatggtt
    14341 tttctaaccg gtatgatcgt caacagagag tcttttatgg aaatggtcca tatatgccaa
    14401 aaaaaaggca agatcgttgt tgccggaggt ccttttgtcc aatcggttta cggcacgaag
    14461 ggaagcaatg acctcgaaat aagcaagatc gatcacctga ttttatatga agcggaaaac
    14521 aatcttccgc aattccttga tgattacgta cagggcaaag cgaagagagt ttacgataac
    14581 aatgaaaaac cttccctcac acagaccccg attccccgct ttgatttaat acgtccgagg
    14641 gattatgtct ccatggccct tcagttttcc agaggctgtc ctttcaattg cgaattttgt
    14701 gatatcattc agatgttcgg ccacatcccc agaaccaaga caccagagca atttatggga
    14761 gaagttgagg cgctttattc aaccggatat cgcgggcgtc tttttgttgt ggatgacaac
    14821 ttcatcgcca atagaaaaaa agttaaagaa ttgctcagat tgctggctgc gtggcaggag
    14881 gagcgtaaat atccctttct gctgtttacc gaagccagta tcgaccttgc gaaggatgat
    14941 gaactgctgg ggctgatgag aaggtcgggg ttcacctccg tttttatcgg gattgagtct
    15001 cccgatgaga agacgctccg tgccactaat aagaatcaaa acgtgacctg cgacatgaat
    15061 gctgccatag caaaggtgca gaaagcaggc atagaagtta tgggaggatt tatcctggga
    15121 ttcgataccg atgcggacga catattcgac agccagttgg aatttatccg gaagaacagc
    15181 attgtccaga gcatggtggg tcttcttgcc gccatgccgc ataccgattt gtacaatcgg
    15241 ttggagcggg aaggaaggct tgtccttggt gttaatcata gcggaaataa tgttgatacc
    15301 gacctcaact tcattcctaa aatgccggtg aagcagctta ttgaaggcta cagaagagtc
    15361 atcaaagaaa cctactcgcc ccgtaattat ttcgcccgcg cgtcaacgct tttgaaaaaa
    15421 tttccggata tgaggatgtc aaaactgaag tacgcggaac cgtggttaaa aaaattatat
    15481 atgtccaaaa tgaccagtca tccgaataag tcaaagatag tctttgaaat tatgaagctt
    15541 gtatttaaac ctttcggcat ttatggattg gtttttatca tcaaatcgtt acgattcgga
    15601 ttgctttcca tgcccgtcgc gatcgaactg actttccgcg gcctccatta catgagcgtc
    15661 gccagagaaa ttgaaaacgc ggctgattaa cggtctgaag cagcaacggt aaaaaaagac
    15721 cctgtatccg gaaggcgtgc cggatcatca gccgtggttt cagggcggga cgcccggtga
    15781 atgaacaaaa aatgtccttg cccgtttggt acggttttct ttgccgattg tcacccttag
    15841 gattttcttg acccttacga aattcattta tatattcaga caataaaccc taacgacttt
    15901 tcaatgcgga gccctcacat gaaacgaatg attcctctgt ttcttttact ggtctgcatg
    15961 acgcttgccg gttgtgccgg tgtcaaggtg tcgtttgtcg gcagcggcga ctacatgtcg
    16021 cttcgccgtg gtgacatcct gacgaccggt gaattgagca cctacacggg cgcctctttg
    16081 caggtggtcg gtatcgataa gaaagtatgt gacaaggacg cttccggatg ccgcaaggcg
    16141 ttgatggaaa ccatcgggtt acacagtgag aatcgtttat cggcgctggc tgaactctgg
    16201 ctgaaagacg ccatggagct cgaaaagcaa ttcatcgaaa gcggacatat cgaagcgacc
    16261 ctgaatgctt acctggaaac cgcgagatac tcctatgcct atctttttct gacaaaacgc
    16321 acgcccaata tgcgggcgct ggaggatcga cagacacagg ttcgggacta ttacaactat
    16381 gccgttgaaa aaactctgac aaccttattt gaatattatc gtaaacgtct gccgcccgat
    16441 tttaattttg agaaggatac gcattttgaa tcgggacaat ggcacatcac cattcgaaat
    16501 gaggacctgc gtttggctga agagcacatt tttccggagg atttaattcc cgcatcttca
    16561 ctgtcgtttg ccggtcttcg caatcaatat cgccgggacg gcttgggggc ggaactggtg
    16621 gccgtgacct ccactcgcgt cgttagccgg caaagcgcag aaatgccctt cagtgaaacg
    16681 ccctttcctc cggttactgc tgtgatgagt tttccgggga acacctggat ggacgtgttg
    16741 aaaaccaacg aggtggtcat cacggggtac gatccttaca gccgcgagtc gttcaagctg
    16801 gtcggcaagg aggtgccgct ggcggccaat ttcacgtccg gttacggact gtggcttgcc
    16861 cgatccggct ttgccgctca gtctcttctg acgcttatcg ggaaaggcga tgtgctggag
    16921 atgccgcgcg tttatctcat gcagccatac aatccccaac gccacatcgt tatcatgctg
    16981 cacggtctgg ccagcagccc ggaagcgtgg atcaatctgg ccaacgaggt gttgggcgac
    17041 gagactctga ggaaaaattt tcagatctgg caggtgtatt atccgaccaa tctgcccatt
    17101 gcgtttaacc attatgaaat cagcaaggcc atcacacaga ccctgaaaca ttttgatccc
    17161 gaaggaaaag cgcgggcgtc tcagaaaatt ttactgattg gacacagtat gggcgggatt
    17221 ctatcccggt tgatggtttc ttcctctgga gaccggttgt ggggtgcatt gcttaaaaaa
    17281 tatcctcttg aagggagacg gctcgagcgt gtccggagag agctgggacc ctatatgagt
    17341 tttgaaccgc tgcctcagat cagccgggcg atctttgtcg ccgcgccgca caaggggacg
    17401 cctgtcgcgg aaatagcgct tgtccgctgg atagccaatt tcatcaaact gcctttctct
    17461 gtgctgggca ggttcaggga agtggcacaa ctgctggtcg atcccggttc cgccaatccc
    17521 gtctcgctga cccggtcatt caacagcatc gacaatctca gtaaccgcga ccccttcatt
    17581 cagctgacct ccacgttgcc ggtttcatcc cgggtgcgtt accattccat tatcggtaat
    17641 aacactccga agaagccttt agccgaatcc agtgacagtg tggtgcctta ttccagctcg
    17701 caccttgagg gtgccgtctc ggaaaaggtg attgcttccg gacatagcgt gcaggaaatg
    17761 ccggacgcta tcatcgagat ccgccggatc atgcatctgc acctgaaaga ataaaaaaac
    17821 atcggcaaga cctgttgaaa atagttttgc gatgaccttg cctggtctcc cgccgcggcc
    17881 ggttggacgt gttatctgga tgatgcatcc cgacccgcga tgaaaaaaat cccgatgaca
    17941 gggcgttaat ttttttaaat aaattttcgg ttttccctgc taaatcgctt caatggttga
    18001 tgtcggtttg gacgtgatgt tgtaggtgac gctgaccacg tccgggatct cgtttgtgat
    18061 tttcgcggcg atgccttcca gaatagcata ggacagtctg gtcggccgtg cggtgcgtgc
    18121 gtcaaggctg ttccagcacc ggatttcaat ctgattgccg aatacgcgtt tgttgttgcg
    18181 catgccggtg acgcgatctt catgcaaaat ggccaaatac tgaaacgcct tgacatcttt
    18241 cagcgccgct tcggtgatcg cggttgcttt tttgaccaga gcgattctgg ccggtgtgac
    18301 ttcgccgata atcctggctg ccagcgccgg tccggggaag gggattcttg caaacatgtc
    18361 ttccggcaga cccagccctc ggccgacttt ccggacgccg tccttgcgca gttcaatgag
    18421 cggttccaga attttgtagc cgaaagcttt ttgcggatcg atacccaatt gctcgaaaac
    18481 gttgtgctgt cttttgatgc ccgctacggt ttcatccacg tccgtcagaa tggtcccctg
    18541 caacaggtat ctggctttgc tcttgacgac cagcttgccg aaaacgtttt tataaaaggt
    18601 ttgcgtaagg gcttctcttt tttcttccgg atcggtcagc cccttgagag ccgagaaaaa
    18661 cgctctggac gcgtcgaaaa cgtcgacctg aatcccaagc tttttaaagg ccgccgccac
    18721 tttggccggc tcattttccc gcataatgcc gttgtcaatg atatacgttt tcaggcgttt
    18781 acccagcgct ttatgtccca gggccgtcac gaccgcggaa tcgacgcctc ccgacagggc
    18841 gttgatggcg aatccatcgc ccacggcctg ccggatgtcc cgtactttat cgtcgataaa
    18901 tttatttacg ttcatatttt ttgcctttat ttctttcatc ttgatcatgg cgactccttt
    18961 gaggatagaa ttataatgca aaattcttta aacgaaatcg cgccgggttt caagtaataa
    19021 agcataagaa gaaaaaatag tttcctaggc tgcgacggct ttagcttttt ggcgttctga
    19081 aaaaactctc cacccgtgaa aaacaatcat aatcgtccac actattaatg gaaatttaaa
    19141 ccagagatat tgggggtata agtcgaaatt aatgaaaatc aaaaaagcat tgatgatcag
    19201 aaatgaaaaa agatgataat agaatccctt gtgagccttt attaaagacg aaaagacact
    19261 gaacgcgtga tatgaaaggg ctacggccca cccagcaaga ggccacttgg cccagaaata
    19321 ttcaggcgac gtatcaagat tgatccagac caatacagca ttgaccgcag caaacgctgc
    19381 taaatgatac cctaatccga tttttgctct tctgcttgtc atacaaatct cctctcctat
    19441 ttttttgcga ctgttgtcag acatgcatca aagtcgtgcc tgcgatccgt gtccgagaaa
    19501 atccttattt ctttaatcct gagaccagtt cctcgacttt cccgttaacg gccttaatgg
    19561 tgtcatatac gtgtttccgt tttccgcctt ccgcaaaacc gctgcgggaa acacctttta
    19621 cgttctcggc aatgcttttg tgaatgcttt cagttgttga aaccacgtca tcaatgcaac
    19681 cgaaaaccaa tttgacgata gcgtgcttct ctttcataag acctcctttt taaaatcatt
    19741 aaccgcaggc cggcatcatc gtgccgagct cgatgatgca aatccttttc tccgaccggc
    19801 aaatgtaata tacacacagc aattataatt gtcaataaaa aaagcaaaat aaatagcaac
    19861 aattataact ataaaatatg gctatttata ttgacaagat atgagtatta tattatagca
    19921 ataataaaaa ttcagaacat gggttcaggg aggcatgatg gctattataa ggaaagagct
    19981 aaaggaaaaa tcaaaaaacg tattgttgcg ggtcagcata ccggcgccgc tgatggctga
    20041 aatcaagaag atcaaaaaac tctgcaggga tacgggtttc tattttgata tcaaacccga
    20101 tgtcatcagc gcagttgaac aagccgttga agaagcgaag gcattaatcc agaaagagaa
    20161 aaaatccaga tagatcaata cgcaccggtg accggaatta agacgagtga caagcacaac
    20221 gatggaaaat cttaaaaaag gcaagaaagc gctggttgtc gaaggcggcg gcatgcgagg
    20281 cgtttttgcc gcgggagttc tcaacgcctt cggcagcggg ggattcgatc cgttcgatat
    20341 gtatctgggc gtttccgccg gcgcctgcaa tcttgcctcg catctggccg gccagaacga
    20401 cagaaattat gacatcatca aacgctattc catagacggc cggttcatca atctgggaag
    20461 atttctgcgc ggcggacacc tgatggatct ggactggctg tgggacgtta ccatcagcga
    20521 atatcgtctg gacctgaaga aactattcgg gcgattaaaa gaacgagaga aagaatacat
    20581 cattgcggta acatctgtcg cgacgggaaa atcagcgtat ctcgaaccgg atgaggacac
    20641 gctggaacat tatctgaaag tgtccagctc gctgccgata ttttatagaa attttctgga
    20701 ggtccattcc gaaaaaatga cggacggcgg cgttacggat tctctgccgg tgcaagaagc
    20761 catccggcgc agggcaaccc agataacggt tatcagaacc aggccgtcgc aatacatcaa
    20821 gaaaaagagt atgttttcca tgtgctatcc tattattttc agggattatc cgctgtttgc
    20881 gcaagcgatg aaaaaaagac ccgggaatta catggaatcg gtgcgtttaa tcagacatcc
    20941 accggcggga gtgcgcgttt ttgaaatagc gccgcccccg gacatcgatg tcggcaggat
    21001 gacgaccgat atcaaacgtc ttcgcgccgc ctatcaaagc ggcagagatc acggcagaaa
    21061 atttatggac gcgcatgcgc cttgaaaatt gcccgatcag accgtctgac aaattatcag
    21121 gacatcggaa atcgtaaacc tgaacaaagc aaagacttga tctccataag ccttcaccgg
    21181 acggatgaac atttataatg taatgaacgt gcggatggcc ggttaaagag ccttttgctt
    21241 gttatttatt tttattctca actccttgaa tttcttcatt taaaattttc ttccgtctct
    21301 tgactttatc tatatattgt ggttaataaa tcaaattcat acaatatata gtatcttttt
    21361 ctttatctct ttcgatattt atgaggaatc gctttatgga acagaaacat ctcgccccct
    21421 ttactccgga attgacgcag aatgctttaa ccgttctgga acgacgatat cttaaacgcg
    21481 acaaatcggg aaaaattctt gagacgccct cggaaatgtt ttatcgcgtg gcggacacga
    21541 tagccgccgc tgacaagaaa tttaacaaga aggcggatac gcgtaaacgc gccgaagaat
    21601 tttacgccat gatggcctct ctggaatttc tgcccaattc gccgacctta atgaatgccg
    21661 gacgcgaact gggacaattg tccgcctgct ttgttctgcc ggtgggcgat tccatggatg
    21721 aaattttcga cgccgtgaag ttcaccgcac tgattcataa atcaggcggc ggcaccggat
    21781 ttgctttttc acgcctgcgg cccgccaatg atgtcgtttt aacgacaacc ggtatttcca
    21841 gcggcccgat ttcatttatg cgtgtttttg acgtagcgac ggaaaccatc aaacagggcg
    21901 gcacccggcg cggcgccaat atgggcattc tgcgggtgga tcatccggat atcatggatt
    21961 tcatcatgtg caaggccgac aaaaaacaat tgaacaattt caacatttcc gtcggtctga
    22021 ccgaagcctt tatgacggcc gttgaaagag acgaaaatta tgacctgatc aatccgcggg
    22081 ataagcaggt caccggacac ctcaatgcgc gcaaagtttt caacaggatt atcaatcaag
    22141 cctgggaaaa cggcgagccg ggcattgtct ttctcgaccg gctcaaccgc gacaatccaa
    22201 ccccgcaggc gggcgaaatc gaatcaacca atccctgcgg cgagcagccg cttctgccct
    22261 atgaatcctg caatctgggt tccatcaatc tggccaaaat ggtgaaagac ggcaagatcg
    22321 actgggcaag actcaaagag atagtgcgcc ttgccgtgca ttttctggat aacgttgtcg
    22381 aagtgaataa atatcctctg ccgcagatcg cccagatgac catggccaac cgtaaaattg
    22441 gcctgggcgt gatgggatgg gcagatatgc tgattaaact gggaattccc tacaatacgg
    22501 atgaagccat ggaactggct gaaaaagtaa tgaagtttat taacgagcag ggacacggcg
    22561 catcgcagca actggccagg aagcgcggtc catttcccaa tttcaaaggt tccatttatg
    22621 ataaaaaagg acagccgccc ctgcgcaatg ccaccgtgac caccatcgca cccaccggca
    22681 ccatatcgat tatcgctaat gcttcttccg gtgtggaacc gctttttgcc gtttcttacg
    22741 tccgtcaggt catggacaat gacattctgg tggaagttca tccctacttc gaagctctgg
    22801 ccaaagaaag gggattctac tcgccggaat taatgcagcg tattgccgaa catgggacta
    22861 ttcatgatct ggaagaaatc cctgaggata tccgccaata ttttgtcacg gcgcatgaaa
    22921 tatcgccgga catacacatc aggatgcagg ccgcttttca gaaatacacc gataacgccg
    22981 tcagtaaaac cgttaacttt aaaaaagagg cgaccgtcca ggacgtggcc cgggtttatg
    23041 agctggccta taaactggat tgcaagggcg taaccattta tcgcgatgga tccagagatc
    23101 agcaggtttt gtccaaaggg aaaaaagaag aaacagctgt gcaggaagat gccgaccggg
    23161 aacaacatct gaccaagcgc gaacgtccga aaatcctgaa aggctggacc tatcagatgc
    23221 aaaccggttg cggaccgctt tacataacca ttaacgaaga cgacaaaggt ttgtttgaac
    23281 ttttcaccac gatgggaaaa tcgggcggat gcgcggcatc acaaagcgaa gccatcggac
    23341 gcatggtttc tctggcttgg cgcagcggac tgtcgtcccg tcaggtgatt aagcagttgc
    23401 agggcatttc gtgtcattcg ccgtccggtt tcggtgaaaa caagattctg tcctgtgccg
    23461 acgctgtggc cagagccatt caatcccaca tcgaagccaa cggcgggaaa gtgcagcacg
    23521 aaaaaatcgt attccgcaaa ggcgcctgtc cggactgcgg cggcattgtg gaacatgaag
    23581 gcggttgtgc tgtttgccgc atgtgcggtt acagcgaatg cgcataaccg atgcatcggg
    23641 acgatgcgtc aacgaccgtc atcaacaatg cattgccggg ctattccact gtcacgttct
    23701 tatttgaaga agatgcggct atcgacaatc gaaacgccct tgccctgttc gtggacgata
    23761 accggattca aatcgatttc ggcgatgtcc ggaaccgctg ccagaaggtt ggacacacgg
    23821 caaatcacat cggcaaaagc gtcgatatcc gccttcattt ttcctctggc cccttgcaga
    23881 atcgggtagg cctgtaaatt tttcagcata tccaacgctt cgcttttcgt taccggagcg
    23941 agacgtatag acgtatccct gaagatttcc agaaaaattc cgccgagtcc cgcaatcaca
    24001 atcggcccga agacggggtc ctgtcgtccg ccgacaaaac attccacgcc gtcggcagac
    24061 atttcctgaa tcaggaaacc atccatggcc ggtcgcggct tcatttcctt tagagatttt
    24121 ttaatgtcgg aaatcgcttt tttgagctcc cgctgatttt tgatattcag gcgcacgccg
    24181 ccgatgtcgg atttgtgcga cgcgtcgcgg gaaagcagtt tcacggcaac cgggaaatga
    24241 agagaaacgt ctccggcctc tttcgcggat ttgaccgtcg cgccggcaat gcttttgatg
    24301 cctgcggctt cgactatgcg tagggattca tcggttaaag gaattctttt ttgcgttttg
    24361 catttgtctt ggatgatatc gatcgctgcc atatcaagtg ttgtctgaac agctctgcct
    24421 ctggcctccc gcgccgtttt ttcctcgtaa taggtggccg acatgttcag cgctttggcg
    24481 gccgtcagtg gcgtagtaaa aatcggataa ctctgattct tggaaatatt caccagttcc
    24541 tgcgtatccg tgaccatggt catggccaca ggcttttgat attgctgtac aagtttgccg
    24601 acattgctca ggaaatcccg cgacattttc ccctcatagt cggctatgta gaggtgattg
    24661 aacaaaacgc catccacatc gggcagtttc agggcttctt ccagaatttg ggtaaaaatg
    24721 gtgtaatcga aaatttctcc cagatcgagc ggattctgat gggcgatgac gcgcgtgttg
    24781 tagatggttt tcagattttc aataaaagcc ggaggaaaat caacaagcga aaatccgaat
    24841 ttcgcgcagg catccacgct catcacggca tgtccgccgg atcgcgacag gatcgcaacc
    24901 cgtctgcttt tcatcaatgg cagccggata attttcaccg cgttgatgaa atcaatatca
    24961 tcgtcgacgc gaatgaccgc cgtttgccgg aaagcgccgt cgaccacctg atcatcggcg
    25021 gagagggcgg tggtatggga ctgggcgatt ttcgccgtga gatcactgcg gttggatttc
    25081 agaacaatca ggggcttgtc ggattggacc gccagatcaa aaaaggctct gccgcgcttg
    25141 aatccttcca gatacatggc gacgacgtcc gttttgtcgt cattgagcag atactccaga
    25201 acatccactt catcgatctg aagcttattg ccgatggcgg caaacttgcc ggggatgatc
    25261 gcgttatcgc cgaattcgcc caggtaggac cctcccacgc cgccgctttg aacaatcatg
    25321 gccactcgcc cgccgggcgc tgtcctggga aagaagccga aaggcatcat cattttgata
    25381 tcgaaattga tggtgccgat gcagttgggg ccgatgaaac gcataccgta gcggtcggcg
    25441 atttccagca cctttttttc caagacattt tgtccttgcg agtattcgct gaatccgccc
    25501 gattcaatca caagccgctt gatgcccttt ttaccgcagg cgtccatgaa atccggaact
    25561 gtttcggcgg gagcaatgat gatggcgaca tccggcacct ccggaatttt ttcgacgctg
    25621 tcgtaaacag gcgcacccgc gatatcgcct tcttcacggc cgacgccgta gatgttcccc
    25681 tcgtagttgt gctgacggtt gttcagaacg ataatatgac ccagattaag ccgtttgacg
    25741 gatgccccaa tgaccaccat gctgcgcgga taaaaaaact tttccatttt tctgtgcggt
    25801 tcctccctag ttacacaatt cggaatctcc aagaatcatt tgccgatttt ggatttaatc
    25861 cttttttgat attccacaat ttcatttctt aaaatattca gatcggcgat gcggtcgtcc
    25921 agtctgcgca actggttatt caaaagctcc agcaggcgtc cgagcacctt gtcgttggat
    25981 ttttcaatgg tccacatggc ctccagctcc tgcatctccg ataatgtcat gcccatgatc
    26041 ttcaggcgct tgatcagttt cagacggcgg aaatcggctt cggtataaat ccggcgtccc
    26101 ccctcaacgc gcttgatgga attgagcagg ccgatttctt cataatagcg gatagtgcgc
    26161 tggctcatat ccagtttctt agcgatttct ccgatagagg tgaaggtttc ttgcgtgtct
    26221 tgcatacgat tcaccccccg ttaaattgat gagctgagct gttctttcag ctcccgtttt
    26281 aaaatttttc ccgaaggatt cttgggcagc cggtcggtga tgaaaacttt cttcggacat
    26341 ttaaaaccgg ccagacgatc cttgcagaat gagataatgt ctttttcctg aatcgacaca
    26401 ccctttttca ggacggcgac caccgccaca acctctatcc atttgggatc gggcaggccg
    26461 ataacggcga cctcttcgat gcccggatac tgataaagga cttcctcgac ttcgcgcgaa
    26521 gcgacgtttt ctccgcccgt tttgatcatg tcttttttgc ggtcgacgac atagagatag
    26581 ccgtcgttgt caaatcgtcc cagatcgccg ctgtggaacc agccgaactg gaaggcttcc
    26641 gctgttttct ccggatcatt cagataaccg gacatgacat gaccggagcg atgaacaatt
    26701 tcccccacgt cgcccagtgc aacaaatttt ccgtcatcat ccatcagccg ggtttccaca
    26761 tttaaaacgg gtttgccggc ggaaccgggc ttcaggagct gatcttcggg cttcaggatg
    26821 gttgccaccg gccccatctc cgtctgccca tagtaattcc agagcttcaa gcctttaaat
    26881 gtgacggaca actgtttgat gatttccacc ggcatgatgc tggccccgta ggcggctttt
    26941 ttcaggctgc cgtggttgta gttgttgaat tccggatgat tcaggatggc gatccagacg
    27001 gtgggcggag caaacatgtg tgtaatctga tatttttcga tcagccggat catctccaca
    27061 ggatcggcct tatgcatgat cacgttggtt gcgccgacat acagataagg catcaggaaa
    27121 caatggagct gagcgcaatg aaacaggggc agggcgtgga gactgacgtc gtcagtctca
    27181 taccggccgt caaaaatgca gctgtgatac tgcgacatca atgccctgtg agacagcatg
    27241 gcgcctttgg gtttggcctc cgtcccgctg gtgtagggaa tctgcacgat atcttctgcg
    27301 ctgacctcca cctgcggttc gtcataattc atcgccgaca tctcggcgat taaattgaag
    27361 aacccgtccg gaatgccaga atcattcagc ggaatgaatc cccatttttc cacggcgggg
    27421 aatttatcct tgcatccggc gatgaccgga tagagcgtat cttcgatgat gaataaacgg
    27481 gaaccggaat gattgatgat gaaggcaatt tcttccgcgt tgagcatgta attgatggga
    27541 acctggatgg cgccgatttt ggccagtccc atcatgctga tcggataata atgggagtta
    27601 taggcgtaaa cggcgacccg gtctcccttc cggacaccgt agcgggtcat cagattggca
    27661 aagcgccgcg cctcccgttc cagatcgtag aatgaaagct tgatattgcg gaaaataatg
    27721 gcggttttat cgccgtattt tgtcgccgcc ctcctcgcca tgtcgccgat agtatcgcgg
    27781 ttgatgatgt tgctcatttt cttacctttc tccctgatag tttttaagca ggatattttt
    27841 tttcaaacac ttccttcatc ttgaatttct gaatctttcc gctggccgtt gtcggatagt
    27901 cgtcaacaaa ctcccagtat ttgggaattt tgtgccgggc gatctttccc tgacaaaact
    27961 cggtgaattc ttcaggcgtg gccgtctgtc cggccttgag ttgaatggcg gccaggacct
    28021 gttctccgta ttttttatcc gggatgccga tgacctgaac gtttacgact ttgggatgcg
    28081 tgaaaagaaa ttcctcaagc tcacgcggat aaatattttc tccgccgcgg ataatcatat
    28141 ccttaatacg gccggtcact ttgaaataac cgttatcgtc catctctccg agatcgcctg
    28201 tgtgtaacca gttgtatcgg tcgatggcgt cagccgtggc atcgggcatt ttgtagtagc
    28261 ccttcatgac acattcgctg cgggtgacaa tttcgccctg cgcattggga ggcaaatcaa
    28321 tgccgagatc ggggtcaaca atctttccct cgatatccgg cagaagccgt cccacggtgg
    28381 agacgcgcaa ttccacagga tcgtcgcgcc gggtcatggt catgaccggc gccgattcgg
    28441 tctggccgaa ggcgatgaca atttcggggc aatgcatttt cgtgcgcacc tggttcatgg
    28501 tttcgacagg acagggagcg cccgccatga tgcccgtccg cagagaagac atatcatatt
    28561 tgtcgaaatc ggggtgattc agaatcgcga tgaacatggt cggcacgccg tttacggccg
    28621 tgcagtgttc tgcggaaatc cattgaacag ctttgagggg atcgaaatat tccacgacga
    28681 ccatggtgga gccgtgataa acgcaattaa gcgaactcat tacacagccg aaacagtgaa
    28741 acagcggtac ctggatcagc agcttgtctt tttccgtcat gcccatgacg tcgccgacca
    28801 tgcgtgcatt gttcacgatg ttatgatgcg tcagcatgac acccttgggg aatccggttg
    28861 tgcccgaggt gtactgcata ttgatgacat cgtcatcatg gaggctgtgg agcctatcct
    28921 ccagctgttt atcggtcacc tccctgccca tggtgatgat gtcgctgaaa tgcagcatgc
    28981 ccggggtgct ttcccgcttg ccgatgtaga taatattttt cagagcgggg agttgtgccg
    29041 aaaccggggt tcccggctgg tggtgatcca gttcgggcaa tattttgcgg acgttttcgt
    29101 aaaaattaac ttctctatag gactccatta aaaaaagcgt cgtggaatcc gattgcttga
    29161 gaatgtattc cagttcgtgc gtctgataat ttgtattgac ggttaccaga atgccgcccg
    29221 tcattcccag cgcaaattgc agatgaatcc agtcgggaat attggttgtc cagacggcga
    29281 cattatcccc gcgtttgatt cccagggcga taagcccttt ggcgacgtcc cggcacgtct
    29341 ggtaaaattc actgtacgtc tgacgaatgc cgaaatccgg acagacaagc gccgggtttt
    29401 gcgggtagca ctcggctgtt tccctgacaa gttggccgat tgttttcttc acaaaatttg
    29461 acatttaata cctcctcgaa gacgtttgat gacttttgta tgatcaaact acttcatgtc
    29521 ctttacgcaa acagactatc ggacataata ctataatatt acgatcgttt tttatgttaa
    29581 gtccagctta cgttaactgg aatgatatga ccggcagatg attttgtcaa ggcaatttta
    29641 tagtttttta tacggtaaga aaatctttta ttttttaatc ggcagccggc ggacacgctt
    29701 ttgctgtaca gattcaagag cagatcttcg gacaggacgt ttggattgaa attgagtttg
    29761 ctggtttgtt tcccgttgca gactttttgg ggtgtaacgt aagaatttat cctcgcagac
    29821 aatttccttg acacagacag gcatgttacc tatcttttag ccgcaaaaaa taaatatttt
    29881 cattttatgc tgcggttttt tattgctgtt ttcagttcag aaaggacaat gctgtgatac
    29941 caaaaataat ccatcagacc tggaaatcaa ccgagatccc tccgcgctgg caaagattca
    30001 gggacgcatg gaaaaaattt catccgggat gggaattcag gctttggacg gacgaagaca
    30061 atcgtcgttt ggtggaaaga gattatacgg cttttctgca actttacgac tcctatgcgt
    30121 atccggttca aaaagcggat tttgccagaa ttttatacct gcacaaattt ggcggtgttt
    30181 atgccgatct ggatctcgaa ccgctccgcc ccctggacgg gatcatcgat caacctcatc
    30241 tgattctggg ggaagaaaaa gaagggatgg ggaagtccct caacaggtgt gacttcatca
    30301 ttaatgcctt tatggcctcg ccgaaggggc atccgttctg ggaaatgctg cttgagcgcc
    30361 ttcgggtgac attccgtcac aaaaggctat tgcaatccaa aggccattac atcatacaga
    30421 ccgtcatttt tgagctggac aaactggcca gagactatca gcaaacgcat caggacatca
    30481 cgatttatcc gtatgaggtg ttttatccgc tgtcctggca cgaagcgtgt tccgaattgg
    30541 aacgaagcaa ggcagttgaa atgtcctata cgattcatca ttttgacaca acctggtttt
    30601 caggcggtat gatgctgatg aaaaaacttc gccgccgtct gaacatctga cggcatattt
    30661 tttatgcaca ggctgtggtt ttaaaatacg atgatgtaac gaaaacaatc tgacggacga
    30721 aagagacggt tatggaaaaa actcttctga taattttttt aattttgttt gtcggccgga
    30781 tcctctggcg atttattttg cagcaactca acatccggca tttgcgccag tgcggaaaaa
    30841 ttattccgcc ggctttccgg aatgtgattg atcaggcaac gctttccaaa atggcggatt
    30901 atacgtatga taatttgcgt ctggagaaca aggaaaattc cacagaagac cttctggaac
    30961 ttgccatcat ttttcttttc ctaccgctgc tggttgcaaa aacaaacgga ttgcagcttc
    31021 acttgatctg gcaggcgctg gttttcttcg gaactctggc gttgattggc ggcgttatcg
    31081 gacttccctt tgacatttac cgcacgtttg ttctggaaaa aaaatacggc ttttccacga
    31141 ttacatggaa gctctggctg accgatctcg tcaagtccgt gatcatttca gcaattctga
    31201 tggggttttt actttccgtt ttcatggctt ttgttcttta cctgccgaca agctggtggt
    31261 tctgggcatg gatatttttt gccgcgttcg agatcctgct tttgtggctt tttcccgtgg
    31321 tcatcgcgcc gctgtttaac aagtacgaac cggtcaagga tgaagaactg aaagagaaaa
    31381 tcacatcact actttccaaa gtcggattga aggccaaagg catttttcag gttgatgaag
    31441 gtaagcgttc caaacatacc aacgcctatt ttacaggtat aggcaaaacc aagcgcatcg
    31501 tacttttcga cacgctgctt gcttcacata cgcatgatga aatactggcg gtgcttgccc
    31561 atgagatcgg tcactggaaa aagaaacata tcctgaagca attaatattt atggttgcgg
    31621 cttcctttgt cggattttat ctggtttatc tgctggtaaa ctggccgccg atctatggcg
    31681 ccttcggttt accgcaaacg cctgtttatg ccggtttgct tctggtgtca ctgtatttca
    31741 gttgcgtcgg attcttcttc agccctctgg ccgcgttcat ctcgcgccga tatgagcgcg
    31801 aagccgacag gatggcacat gaattgaccg gaacgtcaga gccgatgata catgccctca
    31861 aacgtctagc caaagataac ctgtccaatc ttcatcccca tccgtggtat gtgtggtttt
    31921 attattccca tccgccgctt attgaacgaa tagaatatct gaagacaatg gacaaaggga
    31981 atctgcagcg ccaacgataa aaaacattat gatgtcctgt tcatacacga ttcatggatg
    32041 gcgcgcataa cggccaacgg atcggatgca tcgtttacgg cgcggatgac tgccgtattg
    32101 tgcgggcgcg gttgtaattg cctgatacgc tccagggtca gcccgccgat agccacgatg
    32161 ggataacggc tgatggccag cgcggccttc aagatatccg gtcccgtcac cggtgccagc
    32221 cgttccttgg aattggtggc aaacagcggc ccgaacccga tgtaatccac gggtacggca
    32281 tttgcggccg cgacctgctc taggttgtgt gtggaaagac ccagcagcat ggtcggaccg
    32341 atgagctgcc gcacctctgc tgccggaagg tcatcctgtc ccaaatgcac accgtcggct
    32401 tgcgaaataa gtgcgatgtc cggccggtca ttgacgatga agagcgtgcc ggtacctttg
    32461 gtgatttgtc gcagggcacg tgccagagca agatgttctc gttcattatg acctttatag
    32521 cgcaactgaa cggccggaag accggcttcc acagcccacc ggcaaagcgt ttcataaccg
    32581 tcgcgcggat cggtgaggat caggtaaagc ccgtgccgca ggaatgtgcg cattatttgt
    32641 ccctctgccg tattcataag cgcgctagaa tttcttcagc caccttgagt cccgagagca
    32701 gcatgccgcc gaagaccgga cccatgcggt agccgccata gaccgcgtta gcggccatgc
    32761 cgctgacaaa aagtcccgga taaacctcgc gggtattttc gacggtttgc aactctccct
    32821 ttccggcttc catggatttt tcgcccacga tcccgccggt gcttgtgttg agtctcacgc
    32881 ccattttccg tacgagcacc tcggtgacat tggcgggatg gcccgtcccg tcgataacat
    32941 atcgggcatg gacggttagc gggtccacat gccattttag cgtatcaacc gcgccccaat
    33001 taatgaccag accggccacg cgttcgtttt tgattaccac atcctctatg gcgatgaggt
    33061 tgaagacctg caaaccggcc cgcacagcct tgtgaataag cgttgacgtg atatccacgg
    33121 catcaaggac ataatagccc ggcgtatatt cccggccctg gaggtcgaat tcttcaagca
    33181 attgccgtcc ggcctcctgt accacgatgg aattgaacat catgccgcct ccccaaatgc
    33241 ctccaccgat tgagagcttg cgttcgaaaa gagctacctt ccgtcccgct ctggccagat
    33301 agtatccggc caccagcccc gacgggccgc caccgacgat ggcaacatca agatccagac
    33361 agctgcccag ctttttaaaa taggaatcca gaattgcccg actgataatg atttcgttta
    33421 acatgactac ctcccaaaga ttaacctggg cggcttgccg gctggatatc caaaaaaaat
    33481 ccgtttcctc atcaggggaa acggatcaat acgccttttt tcttcctacg ccggcattac
    33541 ccggatcagg ttcaaagggt ataatctcag accgttaagg ccaccccttt tagttgccta
    33601 tccgatggga aagagtttgt caagaggctg atgagcattg atactgactt tttaaaattc
    33661 tttgagaaaa gcgggaaggc ctttgatgct gtccaggacc ttgcaggctc ctgcgaaatc
    33721 gtggttgcac gtgaacttgc tggggatgac aatgcaccgg agtccggcgc ttgtggcggc
    33781 ggccagaccg cgcggcgaat cctcaatgac cagacattcc tccggcgcaa aaccgcttaa
    33841 cgcaattgcc ttgagatacg gtcccggatg cggcttggta taagggcaat ctttcagggt
    33901 caggaaaaac tggaaatagg gcagaagact cgattgccgg tgaatgattt caaagtgctc
    33961 atgtccggaa ctcgtgacga tacccatcaa atattttccc tgaagcgcat ccagcacttc
    34021 ccggacgccg tcaatcacca gcgggtcgcg ggtcagcaaa tcggcataaa gggcgtcacg
    34081 atcccggcga agtttgataa acgcatccgt ccccggggtt gccgccaacc gcatcacgct
    34141 ttctcccctg acaagcgata tttcttcaaa ttgctcccgt tgcaggggaa ttcccaaacc
    34201 tgccaggatt tcctgattgg ccctgaaaaa aaggccttcc gtatcgacca gaacaccgtc
    34261 attgtcccag aaaattgtct tgatcattaa acgccggatc tccatttttg cttgagcact
    34321 ttacacattt gtgttcattc ggtcaatctc aaccatctga aaatcacgat gccggaaagc
    34381 gaaatgtctg ttgtctggtg tgtagagttg gcgcacacac caggtgggca ggaacaacga
    34441 gaaaaatcaa atcgcaagaa atgtttatca tgcgatgaca accattcaga ataaaaaagg
    34501 gtgaccgaaa cagagaggca caatccattt cgtcgcccgg aatatttatg acggctgtaa
    34561 accaaccatt tgttcgatac gcgtggtgac ctgagagcct aatcccagac cttcggccag
    34621 tttttccaga tagtctttct cattctgcgt gtccacttct atggccatca gggaagccgc
    34681 atacatctgc gccgccaggt ccggctgccc ctgtgccgcg gcaatcaatg tgtccgtttc
    34741 catcggttgc tgcagttgca tcatcagata tttctttcct tcctcttcga caccaatctc
    34801 attgaatttt cccatgatcc gattgatttc attttgatcg atcttgccgt cggctttcgt
    34861 ggcattaatc atcgccctca aaaccagttc tgaactctga tccaattgct tttgttcctc
    34921 atccgtaccg ggttcaatca ggccggctgg aatgggagtc ttggcctgac cgatgccttt
    34981 gagcgcatta tacgccagcg ctccgagcag agccatcaag cctccgccca cggcgccgcc
    35041 tgcagatttt ccacctccgc ccataatgga tccgataagc gctcccagac cgccgatggc
    35101 caggtttttg ttgccgccaa ctgccttacc ggcatcaccc aacatattgg aaagtaaatc
    35161 tccgataccg cctgagctgg atgcgcctcc cttctgtccg ccacccagca caccggacag
    35221 taagtcgccg ataccgccgc ttttgccgcc tgagcttttg tctccgccca gcacactgga
    35281 aagcaagcct ccgagtcctc cgctgccgga ctgtcctgcg gcatcgccta acatatcttt
    35341 gagactatcc agttccttgc cgcctccaag agaattcttc atgcgtgcgc tggaagacgg
    35401 tgacataccg gtttgcacca ttgctcctag aagttcgcca atattcattt ctgtaacctc
    35461 cttatgattt caatgctcga aataatgaaa acgatttaat taacctgttc gcttccgaac
    35521 aacttcgcga cgattatttt gttgaccttc tttccctctg gaacaattgc cggtgcttct
    35581 gcggttacag gttcagagtc ggattgtgca atggccggtg tttggggtgc cggaacggga
    35641 aacagcgtta cattacacat ttacatgata ctctttaatc atactgcgtg attatacaat
    35701 aataagaaaa tatatcaaca tttcaaatga tggacgaatt gcctcaaata aaatgttacc
    35761 gaaggtttgc ataaaaggga tacgttgcct cataaaccga tgttaccgag aaaagattcg
    35821 gaaacggagg tttatccatg agtcccaaat cgaaggaaga gtattttgag gttctttata
    35881 agcgatataa agaggcatct cgtaaagaaa agaccgttat catcgatgaa tgttgcgctg
    35941 tttgcggtca tcatcggaaa cacgccatcc gacgattaaa agggtataag cggtttacaa
    36001 agccaaaggc caagaaaagg gggaagcctt ctgtttacaa taaggcacct attatccggc
    36061 cgctgaaaca gatctggctg gcggccaatc ttccctgttc caaaagactc aaggccattt
    36121 tacccttgtg gctgcctaaa tacagcgagc atttcggcgg gctggcagaa gatattatca
    36181 ggactctttg ggccatatcc ccgtcaacca tcgaccggat tctcaagcct atccggattc
    36241 agtatcagaa aagagggaaa tcaacgacca agccgggcac gctgctgcga aagcaaatcc
    36301 ccatcaacac caaccaatgg gatgaatcac ggccgggtta tctcgaagcc gacaccgtcg
    36361 cccattgcgg tgattctctc atggggatgt ttgcctacac cattgacttt gtcgacattg
    36421 ccacgggctg gacagaacaa agatcggtct ggggcaaagg agaaaaaggt gttctggaac
    36481 aaatcaaaga tgtcgaaaaa atgctcccgt ttccgctcct gggttttgat tcagacaatg
    36541 gcagtgagtt tctcaaccat catctcctgc ggcactttac ggatcgtaaa cagcccataa
    36601 actttacccg cagcagggct tatcacaaag acgacaatgc ccacatcgaa caaaaaaact
    36661 ggacgcatgt ccgtcagtgg ctggggtatc aacgattaga caatcccaag gtggtcccgc
    36721 tgatgaacaa tctctaccgc aacgaatggc gcctcttcca taacttcttt ctcccctcgg
    36781 tcaaactcat tgaaaaggaa cgcatcggct ctaaaactat caaaaaacat gatcaaccca
    36841 aaacgcccta tcaaagaatc atggaatcaa aatacatcga tcaatcggtc aaacttgctc
    36901 tctcaaaaca acttgaaaac ctgaaccctt ttgagctaaa taatcttatt caaatgaagc
    36961 tcaaaaagat tgcccgactt cggtaacatc ttttttgagg caacgttctc tccactcgct
    37021 cttctttcgg taacatttat taatgaggca acaggtggat tgcctgcgac gtttcaagag
    37081 gattcttgtt gatcagatac ggcgtggcga tagttatatt tggaaattag tttttcaatt
    37141 tcaatcactc cgttcacatg aatcataacg gctttcaaaa atggcagaag gtatgatcgt
    37201 atcaatcagg gggtaaaaat ccacaaacag acaacttccc ctttttaggg ggtcgaggtt
    37261 gtccttttca gacgccttta cgcttacgct gaaggggcac tgatgagtcg gtgcatggtg
    37321 aatatcagaa aagccggctt ccccgcgtgt ttttagatta ttcaacgcag gaaatttttt
    37381 gaaatgtgga tatctccttg atcgggattc ttttctttaa cagatcttca ggaggcttat
    37441 cttttaagca ataagattgc tccagataaa tatagatggg ggagagtttc gcatgcaatg
    37501 tttttgattc aatcagcatt tcgctgaaaa cgtcacacac ccccgaatct tcctgaataa
    37561 agactctgat ctccgggacg ggaatcgtat cctctttcaa accgtttacg atttcaatat
    37621 agccgatgaa atttttatat tcgtctgtca aattctttgc cggattaaaa acgacgccaa
    37681 cattttcaaa ttccggaacg gaaatattcc ctttatagga aacatgacag gcaacgcgtg
    37741 cgtgagacga cggattttgt ttcccgaaaa aagttgtcaa tttatttttt tccgaaaccg
    37801 taaaactgac gggtaggggc gaattgatca atttgtgaaa cgcattcaac atttcggttg
    37861 tccatgtttt gggaagtttt tgaggttcgt ttgccggcat aattttaatc cttttttgtt
    37921 agagattaat gtagctgcaa attcaataat gcattcccga aaaagaaaac ccattttctc
    37981 tgtgcgagaa agtgggtttt ggattttccc ccgtatggtt catctaatcc actcatgcca
    38041 tttgaaagtt ttatgagcac tagactactg agaaatcatt tcaacattta ttttattcat
    38101 aacctttctt caagaatatt tcaatgaaat agtttttttt accgcgcagg gggatattaa
    38161 atcgaaagct tgatgtttgc agcattattc aggacagtgt ttatctcaga tttggtgctg
    38221 ccggcgctca ctgcctcaat ccaggaatgc gttcattctc cgccttgtgt gctccatcag
    38281 ttcaggcagg tttccgtcgt gataatccgt gacgggaatc ggcttatgaa tgatcatcgt
    38341 cgcaccgccg ggggtcaaat ccaggctgcc tttcggcaga atgtttcggg taccgttgat
    38401 ggtgacggga agaatgggta tgccgaggtc aatggccatc ttaaaggcgc ctttcttaaa
    38461 ttgtttcatc ttcccgtcga cgcttcgcgt tccttccgga aaaaacaaaa cggaggttcc
    38521 gccaacaatt tttgccctgg cggctttgat ggtgtccacg gcacttttcg agtcggatct
    38581 gtcgatgtaa atgtgaccga taattttaca ggccaaacca agaaccggca cttttttcag
    38641 ctctttcttc atcacccatt tgaagtctat gcccagccag ccgtaaagca catagatatc
    38701 atacatgctc tggtgattgg aagtgatgac gtaagacgtg ccgggttcga tgttatccgc
    38761 tccgacgacg tgtacagtca tgggggtgca gaagctgttg attctggccc aaatcattcc
    38821 aaaaattcca cctgtgcgcg gtgagaacag cagcgctgtg acaatggcca aaacgccgca
    38881 gatcagagtg gtcgcaatca ataaaggcag aacaaagagc catttataag gctggtaact
    38941 gatatgggcg atccagtcct ttttctctgc cagggttttg ttcaagccgt cggaaaatct
    39001 ttgccggttg tccagatatc tctgccggta tttttcaaag aattcttttg tttcgcgttc
    39061 caatctcata aatcccccgg tggagattgc agcgtttttc tgatgatcaa agcttgtcct
    39121 tcacttcgtg ccagaataat tcgactcctt ctcgccatcc cttcaggtat ttttccgaaa
    39181 agtcactcca agcctcctgt gcttttttgc ccgttgccgc cacttgcgcc ttatatttct
    39241 taaatatgtc ggagaaataa tcgcctaact cttcatcctg gttgggattt tcgcaagcgg
    39301 tccaggcgag ggcatattga atttcacggt aatgggcggt tttgcaccat tcgaggccgt
    39361 cctttttccc ccattccgcg atgttgagct cgtaatcgac cttttccttt ttcaggcgat
    39421 ccacgataga tgaaaattcg atttcgttcc ggattttcga tgttaaattc tcttttttct
    39481 gtatgatttt ggaaacggca ttctgaaata ctctggaaaa attcagagac gatttccatg
    39541 cctgcatttt ttcataaagc tcgtccggaa cgctgatcgt tattttttgc gccataatac
    39601 atactccaaa atttaataca tatattttaa atagtacata tatgattaaa aaggatgtgt
    39661 caactgcaag ttgttgtgcg aatataatgc cgcaaccaat tcagaaagtt gaagcagttt
    39721 acgctgatcg atagaacaat cagctttcaa caattttgcg taaaagtggt aacgataaat
    39781 ttgagggtaa aatctaaatg gcgccagcag aaaacagatc aagcgacggc atgctgctgg
    39841 ctgattgaaa tgcaaaaaag ctaaattgtg tttttaacgg tatgaacccg aggcaacagc
    39901 tgcgaaaaaa atgcgcgccc gaacataggg gcgcgcatgc aaaaacgaat acgattattt
    39961 ttttccgtag ccgatggttt tagcggcttc tgcaatactt cccagaaccg agggatcgtc
    40021 aatcgtagaa ggaatcacat actccttgcc gtccacgatc ttgcgcatcg tgctgcgcag
    40081 agtttttcct gaacgcgttt tcggcagtgc cttgacgatg gccgcttcct tgaagcaggc
    40141 cagcgcgcca agttctgaac gaaccatttg aaccagttcc ctgacgatat cgtccggatc
    40201 tcggtcgacg ccggctttga gcacgacaaa gcctacggga acctggcctt tgagctgatc
    40261 ttcggcgccg aaaactgcgc attccgcgac atccttgtgc ttcgaaatga tttcttccat
    40321 cgctccggta gacaggcgat gccctgcgac gttgatgacg tcgtcgatgc gtcccatgat
    40381 aaaaatataa ccgtcctgat caaagcgtcc gccgtcgccg gtcacataat aaccgggtct
    40441 ttccatgacg tagtccagat aacgtttgtc atcgttccaa agcgtgggca gacagcccgg
    40501 aggaagagga agtttgataa ccacggaacc ttcttcgccg ttgggaagct ggttgccgtc
    40561 cccgtccaga atctgcacgt tgtaacccgg gacgggtttg gtcggtgagc ccgccttgat
    40621 ggggaatctt tcaatgccca tgcagttggc ggcaatcggc catccggttt ccgtctgcca
    40681 ccagtgatcc accacaggaa ttttcagcat atcgctggcc cagtgatagg tatcgggatc
    40741 gagtcgttcg cccgccagaa aaagatacct aaggcatttg atatcgtatt ttttcagata
    40801 gtcgccgttg gggtcttctt tcttgatggc gcggaacgct gtgggagccg taaatagcac
    40861 agagacgcca tgctgggaaa tgacgcgcca aaaagcacct ggatccggtg ttccgaccgg
    40921 tttgccttcg tagacgatgg ttgtgcagcc ataaagaagc ggtgcataaa taatgtaaga
    40981 atggccgacc acccagccga catcggaggc ggcccagaaa acatcgcccg gtttgacatc
    41041 gtatatattt ttcatagacc atttcagcgc cacggcatga ccgccgttat cgcgcatgac
    41101 ccctttgggt ttgccggttg ttcccgacgt atagagaatg tagaggggat cggtcgcggc
    41161 aacggatacg cagcccgccg ctttggcatt tttcatcacg tcgttaaagt ccagatcgcg
    41221 tgccgaatcc atgtccgcct tgacctgggg acgctggtag ataacgcatt tctgcggttt
    41281 atgagccgca attttaatag cctcatccag tagcggttta taaggaatgg tcttcttccc
    41341 ttcgatgcca caggatgcgg agatgataat tttcggcttt gcatcatcaa tgcgtatggc
    41401 cagttcgttg ggagcaaatc caccgaaaac aacggaatga accgcgccga tgcgtgcgca
    41461 ggaaagcaca gcaaccaggg cttccggaat catgggcatg tagataatga cggtatcgcc
    41521 ttttttgacg cccagggcac taatggctcc ggcaaatttg gatactttgt ccagcagttc
    41581 tttgtaggta attttctgca cggtattggt tacggggctg tcataaataa tggcgttttg
    41641 ttccccccgt cccgattcca cctggtaatc aagggcgtta tagcaggtgt tcaattcgcc
    41701 gccggaaaac cagcggtaaa aaggtttttt gctatcgtca agaaccttgt cccatttttt
    41761 gttccagacg acgtcttcgg ctgctttgcc ccagaaaatg tccggttgat tgattgattc
    41821 agaataaact cccgcatact ttgatttgtc agccatgatg agtcctcttt aaatgaatag
    41881 atttgctatg ggcataacag attgaaatcg aatttgtcaa agataaagat catttctttc
    41941 gtgagacttg aatcccgctt caacggacag atttccgttt gctgtgctca ggggataatt
    42001 cgatcggcca atgtccatac gcgatgcttt aaaattgaag cctgactaaa aaaagcgggg
    42061 caacgatatt tcgttgcccc gcctcattgg attggtcggc agaggatgcc cctctgtcgg
    42121 agataaaaca aaactgttta tttcatgttt ttaaaggagc cgtccacgat ttcgggttcg
    42181 ggtttgaaaa tattcagacc gaaacgggtg tgcagtttat tgagcgtatc tgccagttga
    42241 gcgggttcca tgccggcgcc aaaggcgaac ggaccggcaa acgctctcat gcaatggacc
    42301 atgcccaggt cgatatcggc agcggatgcc gcaatcttct ctttgtaaac acgcacggct
    42361 tcgttgatct gaatggccag aaaatccagc ggaccgaagt cctgagattc tacggacata
    42421 tcgatcttgg ccttgccgcc ttcccaggtg taaatgccct ggccgctctt catgccgagt
    42481 ttcccgctgt ccaacagagt ctgaagtgtc ttgccgggtt tgaattccgg agacagtgtt
    42541 tcggaataat atttgagagt gtgatagaaa acgtcaatac cgacgtagtc agcaagctcg
    42601 aagggaccca tgggcatacc ggccttggcc ttcatgacag catcaatcgt gtccactttg
    42661 attttgcctt catccagaat ggcgctgatc agagcctggt tgggtgctcc gatgcggttc
    42721 acgataaagc ccggggagtc cttgagcact ttgatgggaa cttttccgat tttcttggtc
    42781 agttcgcaaa gcaggtccac gttggcatca gaggtttgct tggcgtaaat cacttctacg
    42841 cctttcatga ccggcgcggg attgaagaaa tgcataccga gaatccgctc cgggttcttc
    42901 ccggccgtgg cgatttcgct aatgctcatg gtggaagtgt tggacgctat cagcgcgtca
    42961 gccggagccg cagccgatat ttcggcacac actgttttct tcagagacat gatttccgga
    43021 acggcttcga taatcacctg aacgtccttg actgcctcgg tattgcttaa agatgtggac
    43081 aaattgccca tgacttcggc ctttttctca gcagtcattt tacccttgcc gaccagagta
    43141 tccagactct tgttgaccgt ggccatacct ttgtcgagaa actcctgttt gatgtccacc
    43201 atgacggcct tgatgccggc ctgtgcgcat acctgggcaa tgccgttccc catagctcct
    43261 gaaccaatta ctgcaatttt cttgatatca tctaacttca ttaccgacct ccttcttctt
    43321 aattgaatta ataagaactt cctgtccaaa tttcattgtg attcttctta tgtttatata
    43381 agaattttgg cagcaattct ttttcgtgtg agcggagtat aggtaaagga gggcgaacct
    43441 gtcaagggaa aaaggattaa tattgtattt aatagggacc ttgcctgcag gggcaaaaaa
    43501 caggaaaaag gccattttgg cgaaggcgaa aagaaaagcc tgccttttaa aatgtttaga
    43561 aaaggacttg ttttgatgat tgcctaaaca ggaacaaatg tatatattaa agaacaaatg
    43621 tcgttttggg gttattcttc aaccgtttca gaatttaaaa aaggactgta cgtttaagga
    43681 ggttcggtat gaattctttg aaaaaggcta ttttcatcgt ttgtttcttc aacctcttgg
    43741 gtttgtggtc tttgtccctt tccggggttg ccattgcaaa ccaaaatgag caagaagcct
    43801 ctcttgaaaa atgcatcaag tcctgcgaag acagtaagca ggtttgttgg aatatggacc
    43861 ctgaccggag gctttgcgaa gcccaatttc agttgtgcat ggagaactgt aataaaaaat
    43921 acaacgcgcc tgtcgtcgaa gacgtaaaac cgccggagcc taaaaagaca cggacattgc
    43981 attaagggtg ttatgaattt tttgccctgc aggcgtcttt tgtttcgtta caggttcacg
    44041 gcgaggtaaa cttttattcg cagtgtgaaa tgttttatac atgccgcaag gattttttcc
    44101 ggtttcgcga ccaggtattt tattaaaagc ataataaaga cagcccgttg tgttctattg
    44161 acggttgccg gttttctggc acaaattgtg cttttacatt atttactttg tcgttgttat
//
LOCUS       DAOOLB010000012        42940 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_653_length_42940_cov_39.313490, whole genome shotgun sequence.
ACCESSION   DAOOLB010000012 DAOOLB010000000
VERSION     DAOOLB010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 42940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 42940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42940
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_653_length_42940_cov_39.313490"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..1799
                     /locus_tag="PLF77_02920"
     CDS             354..1799
                     /locus_tag="PLF77_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPC07837.1"
                     /translation="MQKNAEHIALISDKQHITYEQLHRAVGEVAGFMQQRGIGKGDKV
                     TVMLPNIPEFVFCYFGIVKNGAVVVPINPLTTPSELIYLLNNSDSKILITQSSQLKKY
                     HEIKDNLSTCRQVIAVDALSPEGQPFPGAESQGASFTPVEIKPEDPAVMIYTAGLTGR
                     PLGAVLTHHNLYSQAVVTQSIAQRTPDDVALALIPLFHAFGATANMLLTIRAGCSIVM
                     MDRLTMDGLFGSIEREKITYISAVPRLFMGMIFYEKASRYDTSSLKLCVTGGSAMPAD
                     FMHVFEEKFNLKLLEGYGLTEASPVCSFNRFDQVYKPGSIGTAITGVEIKIGDDQGRE
                     LPRNQIGELIVRGENVMQGYYKNEAATAAVIKNGWLHTGDLGRMDEDGYIFITGLKKR
                     MIITSGFNVYPLEVETVLNGHPAVKAARVSGKEDLMRGEIVKAYVILKEGAVADDKEI
                     IRYCKTYLSNYKVPREVEFVQEIPASADIEY"
     gene            1894..2835
                     /locus_tag="PLF77_02925"
     CDS             1894..2835
                     /locus_tag="PLF77_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HPC07838.1"
                     /translation="MQKPTGFDFLKKHFIVIMGLAGFVFALAVTDMPTLRDYFHADNI
                     GESYAALQSRVQDHIPLPVVYGEKLNPESIIRLTNKARTDNGLAELAENRLLNQIAEA
                     RARDMLGKQYFAHVSPTGEQASDLAEDVGYAYKIIAENIGKGVFHSNQKIVEGWMQSP
                     GHRDNMLSEDIREMGAAVLEGKMKGRPTYVAVQIFGLQSLPTPHNICVAPSEDLLRDI
                     EVKKAQIAALQNQLDRLKNELDAQQAEIDTDKKYTYDDPQKIQNVNEKIYAYNEKSRW
                     YNKIVGEAKGKTAVLSSMIDEYNRAAQFYRACTEKTP"
     gene            2934..>3409
                     /locus_tag="PLF77_02930"
     CDS             2934..>3409
                     /locus_tag="PLF77_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07839.1"
                     /translation="MGRLTGAENYILKKSDSNFLIALFAVIYFTSQIILGTIFHKIGT
                     LQAFMLQTTLSSGNFKDIASGWIASGQIGIYYQHFYLDYFHPLWYSIFLSLMVARAFK
                     MNHVNPKFNGVVLTPFIAGLCDIFENTMHIYFLSDLDRATPVLVAMSGLATNTKWI"
     assembly_gap    3410..3509
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3754..4992
                     /locus_tag="PLF77_02935"
     CDS             3754..4992
                     /locus_tag="PLF77_02935"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPC07840.1"
                     /translation="MNEKYTAHLKENYGKNILPLSVLENERMIAGSPNEILYFYRKDI
                     PIGKLKESIIKTIEHYNLFSSRLIAIGENKFALQYCTDGAVLNELPPLDAVSRDLSLE
                     DIKKMMVHVKTLPGEPLFAITGIPVKDGILAGISCSHTVADGISMLLFLYAWMCITEG
                     RPFLPPSSQRLFKGLPVDSDKIHRVFLPSLSELSSEIKDKAGHYEKDEKLYTRREFFS
                     DEFLADMKRTAKQENETCSISSNQIIISFLLKKYHDHILRDTDKVRLRNGINLRDLHP
                     DIDPLHLGNATFTSMTEFTRKEIHAMSIAQIANRLKATIAGYRNEKFARELSYLTEYG
                     IEFNAEVLRQHHKAYNSETDINSTNLTHLSDLESLGLSSNIGTILDVSSVVHAGFVIL
                     KEKNGQIFADVTSRYPFVQE"
     gene            complement(4961..5734)
                     /locus_tag="PLF77_02940"
     CDS             complement(4961..5734)
                     /locus_tag="PLF77_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPC07841.1"
                     /translation="MMIIGARAHNLDEAITVSKLHCPYIEISLDDPAVVSGWLPQLRN
                     ILNRNGITFLAHYPNEDNPFDVAILRNTFLPRIVTLMELSQKLDIHKATIHFWIDKRR
                     LPHDLIRDKLNILSRIVDYGNDFGITVCIENLSERADSFAPAFDAIPDLKMTLDIGHG
                     QLLSRQNTSFRFIEEHFPRIAHVHAHDNHGGKTVSDDLHLALGDGSIDYKTIFKRLIA
                     KGYASTITTEVKPQDLLKSKQRLEDCIRSITPAQTGNGL"
     gene            complement(5769..6701)
                     /locus_tag="PLF77_02945"
     CDS             complement(5769..6701)
                     /locus_tag="PLF77_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPC07842.1"
                     /translation="MMNHRHPLHGRSTGMFRRTILCLALILAVASLVACSLINTLFPP
                     PAKPAKIALVLGGGGSKGFAHIGVLKVLEAQQIPVHMVVGTSAGSVVGSLYASGKSAF
                     ELQEIALKMDKDKIIDYDWKIWSGGLIIGDKLENFINTYVKKTPIEKLKIPFYAVATN
                     IATGEETVFARGNTGMAVRASCSVPAVFQPLKIGNNTYVDGGVVSPVAVNVARRNGAD
                     IVIAVDISGGLQRTVPDGMFDSIKKSVDIMYSKIAEYQIKHADVVIRPNMKHISSTEM
                     EKFNEAILEGEKAATLKLPELQQIMTQLKQKRRL"
     gene            6968..9142
                     /locus_tag="PLF77_02950"
     CDS             6968..9142
                     /locus_tag="PLF77_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPC07843.1"
                     /translation="MQTVEKTSYKKDEKKEFTPDEFAKAKTTCEFVIELIKAISRSGY
                     YDSHHPVSQEVKKGLYIAFKKTLGDSSEIMLSCHEYEDKVDIHISGILNEPFNIRKLT
                     QSSTSDLFVPKLKDYFERKSLNSFVIKKNITAEHFESFIDVMSEPIAESADTSKLGEY
                     LTKSLVDRGITEVSTIFKTDIVLSRGKLPWRVSIILRRLAKDLNVVPMFRFATEDKMK
                     VIKKQIVEDIIRPLTNNDLLKDLIVNCDIVVDHVSHFMEVDELEKLIISSLQSDAVVP
                     ISTTVFDIYRDNLMDGVSQEENEQRLERNVYLAKVLNIAAERMIAEKMPDIKNLFEQL
                     FECKIIDSDMLPADMRIDRESAQLADEILSQTENYLENISKASSIEEAESIVLTVKRV
                     IPAFVNAGEWKIIARIVQALNDLSVHHEDMIQESEFLKGMPDSIWEGMEERITDEYIQ
                     GDANLRSKIDEVLLLSNTMCIKTVDLMISKSQEPNILKCAVEILSQKGETGRGWAMDV
                     LGQSNHPLSVLNAALLVIMQVGHKEDSKTVRKFLKHTNASIRARALAAMAKINQQDTA
                     AVIIDALNDEEEKVRHQALSLIEHELPVSVEMLQKIVSFLKERISKKNINANDAGLVC
                     GLLKAAGHLGEVSDRETLENEIIEMAAELLNEKKGILKFMKAEIPREKQDILCACAVS
                     LGKCGGIKSKEFLKTLSRNNNDAVANTAKDAMDILDQKAARQ"
     gene            complement(9182..12076)
                     /locus_tag="PLF77_02955"
     CDS             complement(9182..12076)
                     /locus_tag="PLF77_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC07844.1"
                     /translation="MPTKHPIKSKNKKTSPYPNEFSLLSEEIIAKAGVGIYIVQNRKF
                     VYTSNLFQIITGFTDDDLIGASPLDRVYPEDQENVRQHAVKCLKKEIFEPYEFRLFNK
                     DNQLRWILETVTPIVYKGQPATLGSFMDITERKMTEEALRQSEEKYRTIIENIEDGYV
                     EMDLKGNFIFFNDAISKMHGFTSIELLKMNYTDVMDEENAAKIYAKYNHVFKTGIPEK
                     DFEYQIITKDGQRKFLETSISLINNSAGRIIAFRGIVRDRTQRKQAEEALRQSEEKYR
                     SILENIEEGFFEVDLAGNFTFFNHSLCSLLGYSREETLGMNYRQYTDKDSAQDVFRTF
                     NGIFRTGMPVKEFDWFIIRKNKTRRYVEASIYLKKDSSGKPTGFQGFTRDVTDRIQAE
                     FQKEAALEALRESEEKYRNILENIQEAYFEVDLAGNFTFFNDALCRMTGCSKEELRGT
                     NYTKFSDEENSKKVFQVFNKVFNTGQPTEGFDWLIIRKDSTKRYIEASVSLKKDSHGH
                     PAGFKGVIRDISERKRIEQELNYMATHDALTGLPNRLMFNQLLNQAILSAKRHKKQLA
                     VFFIDLDRFKIINDTLGHDAGDLLLQEIARRFRHSLRAVDVVGRLGGDEFVILIEDFS
                     DFAQVSKVAHKILSAAIKPMVIMSEECRVTASIGISIFPKDGQDEQSLMKNADIAMYF
                     AKEEGKNNFQYYSKDIKTQSNERLSIETNLRRALERDEFYLTYQARLDFKSNQITGVE
                     ALLRWNNPYLGQVTPTQFIPLAEETGLIVPIGRWVLKTACAQNMAWQKQNLPPICMAV
                     NLSLRQLSDENLLNDIRMALNESGMPPQLLELEITESMVMHNPAKLIQLLTKIKDMGV
                     RLAIDDFGTGYSSLAQIKHYPIDTLKVDRSFIRNLPKDSEDKAITEAIITMGKTLSLT
                     VVAEGVETLEQENYLREHVCDEMQGFYFSKPVKADDFAELLRNHTGAHLT"
     gene            complement(12222..12602)
                     /locus_tag="PLF77_02960"
     CDS             complement(12222..12602)
                     /locus_tag="PLF77_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family detoxifying hydrolase"
                     /protein_id="HPC07845.1"
                     /translation="MMKKKIISSDTGPVSGPYSAAVEANDIIFISGQLPVDPATGKII
                     TDIKIATGQILTNMQALLKKTGLDLSHIVKTTIFLKDIKDFPAVNEVYAGFFPKDPPA
                     RSTVEVSVLPRDVPIEIEAIAVRE"
     gene            complement(12624..13529)
                     /gene="nadA"
                     /locus_tag="PLF77_02965"
     CDS             complement(12624..13529)
                     /gene="nadA"
                     /locus_tag="PLF77_02965"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HPC07846.1"
                     /translation="MTIVDRINKLKQEKNAIILAHNYELPEIQDIADFVGDSLGLSME
                     AAKTDADIIVFCGVRFMAETAKILSPQKTVLLPDKDAGCAMADMIEPEGLRALQSRHP
                     GAVTMCYVNSSARVKALCDYCCTSANALPMTRKIAPDHRDIIFVPDQNLARYVSANAQ
                     CHFIIWEGYCPVHTDILPEHIQQAKRLHPQAKVIVHPECKPEITALADIVTSTEGMSR
                     HAQNSPDKEFIIGTETGILHRLKKENPDKFFYPASDKAICADMKKITLEKVLHSLETL
                     SYEITVAPDIMDKARLSIERMLQTT"
     gene            complement(13710..14831)
                     /locus_tag="PLF77_02970"
     CDS             complement(13710..14831)
                     /locus_tag="PLF77_02970"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HPC07847.1"
                     /translation="MNKIYKFFTLIIMTIALVIAAEETANAQIGWQKSAGQSLIDALK
                     VKEPLTFCNEKVPLKENDVRERLEREMLVSLQNSDDVILWLKRAYRYFPHIEMVLREN
                     RLPDDLKYIVIAESALKPLAASHKGAVGFWQFIESTGKRYNITVNSDIDERRNFFIAT
                     DAALAYLKDLYSIFNSWTLAAAAYNMGEDGLKTEILMQQTNNYYLLHLNQETQRYVFR
                     ILAAKIIMSDPEKYGFHLSKDDLYPPLQFDRVEISADNPVPIQVIAAAANTYFKIIKE
                     LNPHLKGYDLPPGRHSVLIPKGTADGFSGRYENLLNQWHIEKENATYTVQKGDSIYTI
                     AKNLNVSVKAIMIWNDLAGVNQKLSPGDKLVIFTRNRFF"
     gene            14986..16524
                     /locus_tag="PLF77_02975"
     CDS             14986..16524
                     /locus_tag="PLF77_02975"
                     /inference="COORDINATES: protein motif:HMM:NF025499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HPC07848.1"
                     /translation="MKKKIKPEAHSLFSGKDLQNMERLRLNLNDVERQLTLHRKGPLF
                     LKLNRPCTDHDGIVSFSSARRRALISFYERESCKYDIIKFVPASGAASRMFSEWFSAS
                     EKGGFGSKALDRLFVANLKKFPFFDLIRQNDPGRKLLAEKDVGRLLKFILSSGGMNFG
                     KRPKALIPFHRYSAREIRTPLEEHVCEAALYTLNRGVCHIHFTLSPGYKSGVARYLKK
                     VIAGYEKRNRIRYNISLSVQSASTNTIAVDKNNLPFRDDQGRIVFRPGGHGALLKNLK
                     HLDADFIFVRNIDNAAPETLWEKIIPHRKMLGGLAIRMQEEIFSHIRKLKNNKPDVSQ
                     IRNIANFCSEQLNIVFAEGFDRQPASEKIRTLLSCLNKPLRVCGMVRNENQPGGGPFW
                     VEEKDGTQTLQIVESAHVDTKQTDQAAIWFGAEYFNPVDMVCCIRDYRGRVFDLSDYV
                     NNDTYLITSKSEKGRQLKALELPGLWNGSMAYWNTVFVKLPIVVFNPVKTVYDLLRPG
                     HSVR"
     gene            complement(16548..17519)
                     /locus_tag="PLF77_02980"
     CDS             complement(16548..17519)
                     /locus_tag="PLF77_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HPC07849.1"
                     /translation="MTKAIALFSGGLDSILAAELVRRQTIDVLCLTLTTPFFNAQKAL
                     AASRQFNLPLEVMDITSEHLKMLRSPRYGYGKNMNPCIDCHTLMLNIAGRKMDQTGSD
                     FIVTGEVLGQRPMSQNKQSLNIVAKNSGYRDFILRPLSARLLEPTKAEKENLIDRSQL
                     LAIQGRGRKQQIILAADFGITSYAPPAGGCLLTDPVFTRRLRDLFAHQTEPEIRDYEL
                     LKHGRHFRIHDTCKIIVGRNSGDNEALKHLAASGDVLFNMAHFPGPLVVVPRDSLCDP
                     QIAAALCVHYSDAPPDALQEVICVRDNQSEIVTAAAASKEDCQRWIL"
     gene            complement(17723..19444)
                     /locus_tag="PLF77_02985"
     CDS             complement(17723..19444)
                     /locus_tag="PLF77_02985"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HPC07850.1"
                     /translation="MFKRYVLALLIVLASTTLAWNIVEASREDTAQIQLKKTAISRKN
                     LHTYTVKEGDVLINIIKSIPGISEKDIPYTYRLIKDMNPHIANIENLEVGQSIFLPEK
                     PMKMKNDRTNVKAPAAAPKTVSAGEFYYKVKRGDTLYKIIRREFKVSETSIPQVVRSI
                     KVLNPTIKNVNKIYVGTMLKLPGKTVFVQSPDDLTPATRKPAISDTAEQYVQEDRIIE
                     IKDKKMMPFEARLALLKQIITQMNGTVTTTGNYYLPIPQAGQVTIDCSKIPLIEFDDN
                     TTVFLDLENRAHSNLKKMISNNWKNYHLVKISNTDDTITILKKVFSVTNKYSILKSDK
                     PLTFGALPPAEVMVDWLIVKPIPGQQSKSVIQGLRQIYENNVLLPKSIKNYAQKNGLI
                     ITEFSEETGIADKPEELYSLPPIPVFPATNAKEFSHALVSHLGLSAEKDVEIQIFDTV
                     KDGFNLSLKADVLVTKGNNKYVIYSQSLSQQFINALKQAGNETIFVSNSDSTKDIMGN
                     VLSGLDIPFTYGTHAFSGLEKNQAPYALKFSGTRINPDLYVVNFDIDPGLRGLLQEVW
                     SARIARY"
     gene            complement(19622..20491)
                     /locus_tag="PLF77_02990"
     CDS             complement(19622..20491)
                     /locus_tag="PLF77_02990"
                     /inference="COORDINATES: protein motif:HMM:NF018798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HPC07851.1"
                     /translation="MPPKPQPEDIAGGQALIEGVMMRHGDKIAAAVRRPDNEIVFQER
                     EYIPLTKRYKVLGWMFIRGTITLFEMMIVGFKALMFSADVALSEEERKPKDWEMYLSL
                     MISFSIAIFFFVVVPAYCFTKMKFYIDNLLLLNILEGCVRLGMFLCFLAATLLMKDMR
                     RVYMYHGAEHKTVFAWENGQDLTVENIKNFSTRHPRCGTSFILFVMIVSILVFSLLGR
                     PDFLHRVLYKILLLPVVSGISYEAIRFTGKYKHSRLVQILSWPGLMLQRITTREPSDD
                     QIEVAIAAMKKVI"
     gene            20631..20849
                     /locus_tag="PLF77_02995"
     CDS             20631..20849
                     /locus_tag="PLF77_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07852.1"
                     /translation="MAQMSSTDLFIREYQDRFEKKIRENEIASLEHWKAQLDKVIAMR
                     PDGVASMQLQITKISEMMSNRIKTLKKE"
     gene            20851..21426
                     /locus_tag="PLF77_03000"
     CDS             20851..21426
                     /locus_tag="PLF77_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPC07853.1"
                     /translation="MDSIANFLFEVGMLSRTPRSGYQFLGSGQESVAEHILRTVFVGY
                     TLCKMDSSLDEMRVLKMCIFHDLPEARTGDMNYVNKKYVKVDEEKAIRELAESLSFGA
                     DIRSAIDEFNQKKTKEALIARDADQIALILQLKECGDLGNKYAEEWIGFAVQRLNTEN
                     GKKLSEKITGTDSSLWWFKEKGDWWVNGNNP"
     gene            21458..21904
                     /locus_tag="PLF77_03005"
     CDS             21458..21904
                     /locus_tag="PLF77_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07854.1"
                     /translation="MIHKKHRVSFFLLVLSVLNLISCFGLGPGGTVISKPDQITRTFE
                     TKETAALRAVARVFSEKKMGADTKIDYENKRVDSDYVVSGQWRTKAAAVVRQINWKEC
                     EVTVSVITEKHAEKGWEMRRLLQHPQYDNLFSVIELKIYEEMSRVN"
     gene            21925..22329
                     /gene="mscL"
                     /locus_tag="PLF77_03010"
     CDS             21925..22329
                     /gene="mscL"
                     /locus_tag="PLF77_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPC07855.1"
                     /translation="MAAKNKVSAMMEEFKNFAFKGNVVDLAIGVIIGAAFGKIVDSLV
                     KHIIMPLVSVVIPGEQGYLGWKWIINGKEVPYGLFIGEIVNFLIVAVALYFFIVKFLG
                     MMMKSKEEEAAGPAPLTKDQELLTDIRELLKK"
     gene            complement(22434..22619)
                     /locus_tag="PLF77_03015"
     CDS             complement(22434..22619)
                     /locus_tag="PLF77_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07856.1"
                     /translation="MATIMPEAENVQKAIKWISASLESENLPLQKLIEKAIFKFDLSP
                     KDTEFLTNFFRESRSNK"
     gene            22802..23374
                     /locus_tag="PLF77_03020"
     CDS             22802..23374
                     /locus_tag="PLF77_03020"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HPC07857.1"
                     /translation="MKKSFGAKTLIYPTPVWLIGTYDQEGRPNAATVAWCGVCSSDPP
                     ALAISLRKSRYTYESIQHRKAFTVNIATADQVVAADYCGMVSGKKTDKFAAAGMTAVH
                     SNIVDAPYIAECPMVVECRLTDQMEVGIHVHLIGEICDVKVETTLLTEDQLPDIMKIQ
                     PLIYAPENRSYHGVGGYLGEAFSIGKKIMK"
     gene            23432..24010
                     /locus_tag="PLF77_03025"
     CDS             23432..24010
                     /locus_tag="PLF77_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPC07858.1"
                     /translation="MAKSIKGTKTEKNLLASFAGEAQARNRYTFFASAAKKEGYEQIS
                     RIFLETAENEKEHAKVFFKYLEGGEVEITASYPAGVIGDTISNLKAAAEGENMEWTVL
                     YSDAAKVAREEGFPDVARSFEQIAKVEMFHENRYRKLAENIAKGEVFKKKTATKWHCI
                     NCGYVFEGEEAPKECPACKHPQAYYEVLAENY"
     gene            24455..25078
                     /locus_tag="PLF77_03030"
     CDS             24455..25078
                     /locus_tag="PLF77_03030"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HPC07859.1"
                     /translation="MSTKKQLRSESIKEKKIKKSTRVFYLSLIVGGGVVLLVIFFITI
                     FSALFPPVDTDVMTKKERRVVKIYFSDQQERFLKAEKRHVIKESDIALQAQEIVKALL
                     DGSKTGLVNTFPKNVSLRDVKIKDHDVAYVNFSKDLIKAYQGSSAAEMNTIYSLTNSI
                     TQNIPEIKRVKILADGKELSSIQGHISTGKFFSPDLELIIPEQSPNN"
     gene            25088..27121
                     /gene="fusA"
                     /locus_tag="PLF77_03035"
     CDS             25088..27121
                     /gene="fusA"
                     /locus_tag="PLF77_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPC07860.1"
                     /translation="MASKSKIARTRNIGIVAHIDAGKTTVTERILYYTGKSYKMGEVH
                     DGEAVMDWMPQEQERGITITSAVTTCVWRNHDIHVIDTPGHVDFTIEVERSLRVLDGA
                     VVVFCAVGGVEPQSETVWHQADKYGVPKIAFINKMDRVGADYFNVIGMMRERFSSLPV
                     AIQIPAGSEDNFRGVLDLINQKMIVWNDSTSGSEMSKQDVPSEFADHVKLQRDKMIET
                     LAEVDDSLADKYLGGEEITIAEIEEALRKATISLKIVPVLCGSALRNKGIQPVLDAII
                     NYLPSPEDIPPVKGMNPVTKEEEIRRSSEKDPLAALAFKIMMDEGRKLTYLRIYSGKI
                     QVNNEIYNPIKKRREKIARLLRMHANKRERIDSASAGDIIAVLGLKSTTTGDTLCAEQ
                     KPILLELIEFYEPVISQAIEAKTPADQEKLTAALDKLVEEDPTLKVKYEDETAQTIIS
                     GMGELHLEIIVDRLLREFNCHVNVGRPRVVYRETIQKKVEIEGIFEKELGEKKHFGHV
                     RIALSPRKRGSGNDVIIKLDDSVIPQDFHEVIEEGIREAMMNGVLNGYPVMDTEVAII
                     GGSFREGESSAQGYKIAASTAFRDGCGQAEPIMLEPIMMVDVITPSEFMGDVIGDINA
                     RKGEIQAVNPKGTVSEIKAKVPLKAMFGYSTDLRSSTQGRAVFSMIFSEYNKI"
     gene            27315..29402
                     /locus_tag="PLF77_03040"
     CDS             27315..29402
                     /locus_tag="PLF77_03040"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HPC07861.1"
                     /translation="MNLKIRKRDGRLVKFNAEKITNAIAKAGNATGDFDIKEARKLTI
                     KVLNLAEKLFDSKILSVEEIQDVVEEVLLSSPYRKTAKAYIIYRDQHSRLRDIANRME
                     VDLVDQYLKKLDWKINENSNMDYSLQGLNNYISSEVSKVYWLNEIYTPEIHKAHTEGD
                     FHIHDLSLLSVYCVGWDLYDLLLQGFQGVSGKVESKPANHLRSALGQVVNFFYTLQGE
                     AAGAQAFSNFDTLLAPFIRYDNLSMKEIKQALQEFIFNINVPTRVGFQTPFTNVTLDV
                     TVPKYYADQNVIIGGKPQKETYREFQEEMNLFNKAFLQVMAEGDAKGRVFTFPIPTYN
                     VTKDFNWDDPNLQDLWEMTAKYGVPYFSNFINSDMNPEDARSMCCRLRIDNRELEMRG
                     GGLFGSNPLTGSIGVITINMPRIAYLSKSKKDFLTRLEHLMELAKESLETKRKVLEKF
                     TYGNLYPYTKYYLRGVKERFGEYWKNHFSTIGLVGMNEACLNLLGVNVATDKGQVFTK
                     QVLDFMRNKLISFQKETGNNYNLESTPAEGTSYRLAKIDKEKFTDIITASEGKSKKAE
                     PFYTNSTQLPVDYTDDIFEALDLQDEIQVKYTGGTVFHIFAGERIDDPQSVKKLVQKI
                     CFNYHLPYITFTPTFSVCPSHGYIKGEQEKCPTCADDCEVYSRVVGYLRPVKQWNKGK
                     QEEFDLRQEFKFK"
     gene            29471..30163
                     /locus_tag="PLF77_03045"
     CDS             29471..30163
                     /locus_tag="PLF77_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HPC07862.1"
                     /translation="MMKIGGLQRVSLIDYPGYISAIIFTQGCNFKCPYCHNPELVNPD
                     LFRTPIKVSEVIDFLESRKGKLDAVSICGGEPTIQDNVISFIRQIKKIGFAVKLDTNG
                     SVPNVLKTLIADNMLDFIAMDVKAPLDKYRDIVKANVNQDAIQESIRLVLNAKIPYEF
                     RTTIVESQLDENDILQIGKMINGAGHYVLQNFMAKKTLDKKFAKEKSFSEEKLRKIKK
                     QLEQQIPSVTIR"
     gene            complement(30261..31388)
                     /gene="hemW"
                     /locus_tag="PLF77_03050"
     CDS             complement(30261..31388)
                     /gene="hemW"
                     /locus_tag="PLF77_03050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPC07863.1"
                     /translation="MRSEKAGLYIHVPFCLSKCAYCDFYSVKTLHLIPDYIAAVQKEM
                     KLYRNVFSSFDSIYIGGGTPSLLAPQYLDEIFTALNKYFIIDKNAEITLEANPGDLSP
                     VYLKALRDTGINRLNIGVQSFNDKILKCLGRRHSAQEAMASINAARKAGFRNLGIDLI
                     YGVYGLGLQGWEKILQQALILAPEHFSCYQLSLHEKTPLHKTYLSAGWHLPDDNAELK
                     FFLTTSEILENVGYRHYEVSNFARKDKYTSRHNQKYWQHVPYLGLGPAANSFLNNQRW
                     WNKASVKRYLNAMARGKTPVEASETLTVNQLRLEALFLGLRCKSGIDLKRFKDQYGTD
                     LMTEKKSIMDALMKNKLIELKNGFLCPTRSGMALADSLALI"
     gene            complement(31427..33223)
                     /gene="lepA"
                     /locus_tag="PLF77_03055"
     CDS             complement(31427..33223)
                     /gene="lepA"
                     /locus_tag="PLF77_03055"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPC07864.1"
                     /translation="MENIRNFSIIAHIDHGKSTLADRLIQFTGVCNERNFQDQILDNM
                     DIERERGITIKSNAISLPYRAKDGKTYILNLIDTPGHVDFSYEVSRSLASCEGVLLLI
                     DAAQGVQAQTLANLYMALEHDLEIIPVINKIDLPSADIDRVLEEIDSELGLDPFTHIK
                     CSAKEGAGIEEVLEAIVLRIPPPKGSPANPLAALIFDAKYDSFRGTIIHCRVFEGTIK
                     SGDIIKFMSNGATYKVEEVGHFLLNRTKREKLSAGDVGYIIAGVKTVSDVRTGDTITL
                     QNRPCVQPLPGFKDVKPVVFASIYPIASDDYQDLADSLEKYKLNDASFIYEKDSSAAL
                     GQGFRCGFLGLLHLDIVQERLEREFNLSIILSVPSVRYRFSLTDKTVIYVDNPAHYPD
                     PIEIDKGEEPYIRSSMMLPERYLGAVMKLCMEKRGVNSVMNYTGPGRVELSYEMPLAE
                     VIYDFYDRFKSVTQGYGSFDYDIIDYRESNLVLMDILVNGEKVDALSQIAHRDRARAR
                     ALLACERLKEEIPRQMFKIAIQGAIGGEIIARTTISAFRKDVTAKCYGGDITRKRKLL
                     EKQKAGKKRMKMIGSVSIPQSAFLAVLKSDTD"
     gene            complement(33291..33965)
                     /locus_tag="PLF77_03060"
     CDS             complement(33291..33965)
                     /locus_tag="PLF77_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HPC07865.1"
                     /translation="MNILIFGPNGSGKGTQGAIVQKKYGVPHIETGVIFRQNISKKTP
                     LGEKAKAFIDRGELVPDDITIPMILDRLKEDDCKKGWLLDGFPRNLAQAEALWDALQK
                     ENIKLDSVIEIVLDRQIAKNRIMGRRLCKIDNNHPNNIFIDAIKPHEHDGKTVCRVCG
                     CEDLSTRADDQDTAAIDKRHGIYYDTKTGTLAAVNFYKAKVKVIEVDGSVGVKEVTDE
                     LMKKLG"
     gene            complement(34355..35797)
                     /locus_tag="PLF77_03065"
     CDS             complement(34355..35797)
                     /locus_tag="PLF77_03065"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPC07866.1"
                     /translation="MCKFIQNQYIYLGMIVLMLLLTSDICRAEFTIEDENKLGREFYE
                     QLEQHQMILHNKVLTDYITGIGNLILDQTTRTPFKFNFFILDSSAINAFATPGGYIYI
                     NKGLLLVAENEAQLAGVIAHEIGHANARHVASIISKSQKLNMAALAAMLAGVFLGGGG
                     EASAAITAFSLAGSSSMTLRYMREHEEEADRLGIDYLVHAGYNPSAMVDFLKIMKQYE
                     FLSKTMPSYLQTHPGTDDRIIYMDGLIMTRYRQPGKNSIIGNLCRVQASVPLSASELS
                     RRQRQLETSLAKDPQNTDLLYNLAMIEDQLGANSSALEHLQKALVQSPDDHEILKSIG
                     LLHLKTGRYEQAQNYLLRAKKSDPRNDEITFALGKTYFSSGKYREALDCFLNIRDRAS
                     NEAANLNYFTAMSYGKLQNKGESHYYFGLHFKQANKKDSALFHFREALNFFPPGSERN
                     AEIHRAVKELTAGKDKQKRTIKKDEQNSFL"
     gene            35892..36560
                     /gene="cmk"
                     /locus_tag="PLF77_03070"
     CDS             35892..36560
                     /gene="cmk"
                     /locus_tag="PLF77_03070"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HPC07867.1"
                     /translation="MKKKAVITIDGPAGAGKSTISKILAKRLGYIYLDTGALYRGLAY
                     KALKIRISIDSSAELSELCSTTKVDLKNVNGRMRVYVDGEDVENRIRTEEVGLIASKI
                     STFVVVREKLLDLQREAGLRGGLVAEGRDMGSVVFQDADYKFYLDATLAERTKRRHKE
                     LLLKGSTAEYQSIQNDMSIRDKQDQERAIAPLKPPEGSIIVDSDNLSIEEVAEKIMSM
                     ISKK"
     gene            36671..38431
                     /locus_tag="PLF77_03075"
     CDS             36671..38431
                     /locus_tag="PLF77_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPC07868.1"
                     /translation="MVNNNNSNLSNKNEAGEKSLPKNNITSGKEEMDFKELYEQSLNQ
                     FQYGDIATGKVVQIKDDRIMVDVGWKTEGFIPIDEVRDAQGNVTISIDDEIDVFIDRR
                     DSEGNLVLSRDKASKVKIWDEIKNACENDTVVEGVVVEKVKGGLSVDIGIIAFLPGSQ
                     VDIRPVKDLDKFVGQTLDFKVLKFDRKRNNVVLSRRSIVASEREAEKQDILKNIQEGN
                     VVEGIIKNITDYGVFIDLGGVDGLLHVTDISWGRIVKPSEMFQKGDKITTKVLSFDAE
                     KERVSLGLKQLTANPWEKITETYPVNSIVRGKVVNLTDYGVFVELASGVEGLVHISEM
                     YWTREIKHPSKVLNIGEEIDVKVLEVNAQNKRISLSLKQTTPNPWEKLKEKYPVGTIV
                     KGIVRNITNFGVFVGIEEKIDGLIHVSDISWKHRVNHPSEYFKKGQEVEAVVLNIDVQ
                     NEKFSLGVKQIEKNPWDELAQKYAPGSVVTGKITNFTDFGIFMEIEEGIEGLVHISEI
                     SQKRVKTSSELYSVGDTVSAVVKSIDPKSKKIRLSIKELEAPAPTPSSNQYINNTENV
                     GSNLSQALADVKINTKQKGE"
     gene            38435..39331
                     /gene="sppA"
                     /locus_tag="PLF77_03080"
     CDS             38435..39331
                     /gene="sppA"
                     /locus_tag="PLF77_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPC07869.1"
                     /translation="MRKHPVLFGMLILFVFGLIFYLSFYNAGFNMGKTSRFSLKNKIG
                     VVPVEGMIADSLEIVEQLDKFGKDGSITAVVLRVDSPGGGVAASQEIYDAILELKKTK
                     KVVVSMGSIAASGGLLIACAADKIVANPGTITGSISAIMQFANFEELLKKVGVKSAVV
                     KSGEYKDIGSPLRDMTPEEKVIVQDLVDDIYNQFVDVIVKERKLSREKVIEIADGRVF
                     SGRKAKELGLIDYLGDMSSAAKLADKLAGGNGEFDLVYPSKKRASIFDYVLEGAASKI
                     SASLKEKMEMNSGLSYLYYPGR"
     gene            39345..39629
                     /locus_tag="PLF77_03085"
     CDS             39345..39629
                     /locus_tag="PLF77_03085"
                     /inference="COORDINATES: protein motif:HMM:NF001222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HPC07870.1"
                     /translation="MTKNDLIKKLQEELKTYSLKDVTYVVNIIFDSMIDAIKRGERIE
                     LRGFGSFEVRERKPRMGRNPKTGTQVKLTERKVPFFKTGKELRIMVDNKK"
     gene            39626..40390
                     /locus_tag="PLF77_03090"
     CDS             39626..40390
                     /locus_tag="PLF77_03090"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPC07871.1"
                     /translation="MTYHSLIVEKKKKYAVIKLNRPHEMNAISKEMHQELYDVFADLE
                     TDSDVRAVVLTGGEYVFSAGMDIKEMNNLPDKESDKFLKSMMMYLKKIYAFKKPVIAA
                     VGGIALGGGFNLVTVCDLVVASESAIFCHPELKFGFNPFFYPLSQIVGITKAKEIVML
                     GEPIGANEAMKLGLVNKVEPPERFMEVAESIADTLSQRSPRAFEELKNLCSIVPRMDK
                     MAAMDIESSICALLFTRDERKEQMREVLSKDKSGRK"
     gene            complement(40626..40775)
                     /locus_tag="PLF77_03095"
                     /pseudo
     CDS             complement(40626..40775)
                     /locus_tag="PLF77_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            complement(41038..41370)
                     /locus_tag="PLF77_03100"
     CDS             complement(41038..41370)
                     /locus_tag="PLF77_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016730 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-thioredoxin reductase catalytic
                     domain-containing protein"
                     /protein_id="HPC07872.1"
                     /translation="MTPAELYDMMKKVQEPKGYFFNKDKDWVLSILGDLLVNKERYGY
                     MCCPCRLAAGNREKDADIICPCQYRPDDVREYGSCFCNLYVSAEWNEGKIEHQYVPER
                     RPKEKIGF"
     gene            complement(41370..41621)
                     /locus_tag="PLF77_03105"
     CDS             complement(41370..41621)
                     /locus_tag="PLF77_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HPC07873.1"
                     /translation="MAAKIKIYTLSTCSHCKATKKFLNDNSLPFDCVDVDLLQGDARQ
                     KMLEEVMQYNPDRSFPTILIGDKVIIGFKENAIKEALGI"
     gene            complement(41689..42312)
                     /locus_tag="PLF77_03110"
     CDS             complement(41689..42312)
                     /locus_tag="PLF77_03110"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPC07874.1"
                     /translation="MEFKDVIKQRRAVNFFDPTKNVTDAQLKQIIETAALAPSGFNLQ
                     PWRVIVLRDKEKKEMLRKVAMNQPKITEAPVVLIVLADREGWKKGNPGFEKDFVESVK
                     EGIMKQEQYDWFCKATNSLYGATPERQLAFACKNTGFFAMALMLAAKDAGLDSHPMDG
                     FDIDGVHKAFNIPDQYWIPCLLAIGHFDKTKTLLPPKWRKSYEEIVY"
     gene            complement(42477..>42940)
                     /locus_tag="PLF77_03115"
     CDS             complement(42477..>42940)
                     /locus_tag="PLF77_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HPC07875.1"
                     /translation="KKSLPPAVAKNSLTRRLVNVIIKNKEKIIEQWLEDVIVNPSTTE
                     YHKVERTVLYNICDKILSQLTLWLGGLHDVDKIRNFYTKLGKERRISGFKISGALSAL
                     SLIRKHIWTFALAQDALQKNLELLMTFELQRRMTVFFDLATFYLTKGHEEK"
BASE COUNT        11619 a         9143 c        10653 g        11425 t
ORIGIN      
        1 gtcttgcagg atctcgaagg tttgcagatc atgcggacgc tggggcaaaa catggcctgg
       61 cttttaaaat ccatcgccgc gggacggaaa gcgggaattg tccggccgga gccggaagaa
      121 cacatatgga cgaattttat ccgatgaccc gatgccgata ttcatgccga cccgaagaaa
      181 catgaataaa tgattagact attgcgggta attgttatac aaaaaacgcc ggatgaaacg
      241 cgagcgaccg gtttttattt tcttgaaaaa tgattcaggg ttatgttaag tgaccggcaa
      301 ttttgggggg aggtttcagg gctattaatc tcattcaatt actcgaagat gcggtgcaaa
      361 aaaacgctga acacatcgct ttgatcagcg acaagcagca tattacttac gaacagctcc
      421 atcgggccgt gggcgaagtt gccggattca tgcagcagcg aggcataggc aagggcgata
      481 aagttaccgt tatgcttccc aatatcccgg agtttgtttt ctgttatttc ggcattgtca
      541 aaaacggggc ggtggttgtt cccatcaatc cgctgacaac cccgtctgaa ttgatttatc
      601 tcctgaataa ttcggattcg aaaattttaa tcacacaatc ctctcagctc aaaaaatatc
      661 acgaaatcaa ggataatctt tccacatgcc ggcaggtcat tgccgtcgat gccttaagtc
      721 cggaaggcca gccttttccg ggcgcggagt cgcagggagc gtccttcaca ccggtggaga
      781 tcaagccgga agatccggcg gttatgatct atactgccgg gttgacgggg agacctctgg
      841 gcgcggtgct gacgcatcac aatttatatt cacaggcggt tgtaacgcaa tccattgccc
      901 agagaacgcc ggatgatgtt gcgctggcgc tgattccgct ttttcatgct ttcggagcca
      961 cggccaatat gcttcttacc atccgggcgg gctgttccat tgtcatgatg gaccggctga
     1021 cgatggatgg tcttttcggt tccatagaaa gggaaaaaat tacgtatatt tcagccgtac
     1081 cgcgcctgtt tatgggaatg attttttacg aaaaagcctc ccggtacgac accagttcgc
     1141 tgaagctttg cgtcaccggc ggatcggcga tgccggccga tttcatgcat gtctttgaag
     1201 aaaaattcaa tctgaaactt ctggaaggtt atggactgac ggaagcgtcg cccgtatgtt
     1261 ctttcaatcg attcgatcag gtttacaagc cgggatccat cgggacggcg ataacaggcg
     1321 ttgagattaa aatcggggat gatcagggac gcgaactgcc ccgcaatcag atcggcgaat
     1381 tgattgtcag gggagagaat gtcatgcagg gctattacaa gaatgaggcg gccacagcgg
     1441 cggtcatcaa aaacggctgg ctgcacacgg gcgatctggg ccggatggat gaagacggtt
     1501 atatttttat caccgggctc aagaaaagaa tgatcatcac gagcggattt aatgtttatc
     1561 cgcttgaagt ggaaactgtg ttgaatgggc atccggcggt gaaagccgcc agagtttcgg
     1621 gcaaggaaga tctgatgcgc ggtgaaatcg tcaaggcata cgttattctg aaagagggag
     1681 cggtcgcgga cgacaaagaa attattcgtt attgcaaaac ctatctttcc aattacaaag
     1741 tcccgcgcga ggtcgagttc gtgcaggaaa ttcccgcttc ggctgatatt gaatactaat
     1801 ggtttttcaa cggcgctgaa cggggcaagc tgcggttgac cttccctttc tttcttcata
     1861 taatttcaca taaaaataaa ggatgatgca gcattgcaga aaccgacagg attcgatttt
     1921 ttgaaaaaac atttcatcgt gatcatgggg ctggccggtt ttgtttttgc gctggccgtt
     1981 acagatatgc cgacgcttcg ggattatttt catgctgaca acatcgggga atcgtatgct
     2041 gctttgcaaa gcagggttca ggatcatatt ccgctgcccg ttgtttacgg agagaaactg
     2101 aatccggaat ccattattcg tctgacaaac aaggcgcgaa ccgataacgg ccttgctgaa
     2161 cttgcggaga atcgcttgtt gaatcaaatc gcagaagcca gagcgaggga tatgctggga
     2221 aaacagtatt ttgcgcatgt ttctcccacg ggcgagcagg catcggacct ggctgaagat
     2281 gtcggttatg cctataagat tatcgcggaa aatatcggga agggcgtttt tcactccaat
     2341 cagaagatcg tcgaggggtg gatgcaaagc cccggacatc gggacaatat gctttccgag
     2401 gacatccggg aaatgggagc ggctgtgctg gaaggaaaaa tgaaaggaag gccgacttac
     2461 gttgccgtcc agatattcgg tttgcagtcg ttgccgacgc cgcacaatat ttgtgttgcg
     2521 ccttcggaag atctcctgcg tgatattgaa gtcaaaaagg cgcagatcgc agctttgcaa
     2581 aaccagctcg accgtctgaa aaacgagctg gacgcgcaac aggcagaaat cgatacggat
     2641 aaaaaataca cgtacgatga tcctcaaaaa attcagaacg tcaatgaaaa aatttacgca
     2701 tataatgaaa agagccgctg gtacaacaaa atcgtcgggg aagccaaagg caaaacggcg
     2761 gtgctgtcct ccatgattga tgaatataac cgagcagcgc agttttatcg ggcatgcacc
     2821 gaaaagacgc catagacggt ttcatccagg cagcttgtga ttgacagatc acgtcaataa
     2881 gattatattt tttccgtttt caaaagcata tcgaaaaact ccggaggccg attatgggaa
     2941 gactgacagg ggctgaaaat tatatactga aaaaatcgga ttccaatttt ctcatcgcgt
     3001 tgtttgccgt catctatttc acatcgcaga ttattctcgg tacgattttt cataaaatag
     3061 gcacgcttca ggcctttatg ctccagacga ccctcagcag cggtaatttc aaggacattg
     3121 cgtccggatg gattgcgtca ggacaaatcg gaatttatta tcagcacttc tatctggatt
     3181 actttcaccc cctctggtac agtattttcc tgagtttgat ggtcgcccgg gcctttaaga
     3241 tgaaccatgt caatccgaaa ttcaacgggg ttgttttgac gccgttcatt gcggggctgt
     3301 gcgatatatt tgaaaacacg atgcacatct atttcctttc ggacctcgac cgggcaacgc
     3361 ccgttctggt tgcaatgtcg gggctggcca ccaatacaaa atggattttn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc aacgcccgtt ctggttgcaa tgtcggggct
     3541 ggccaccaat acaaaatgga ttttgtctct tttggtcacg gccctggcca ttgttctgat
     3601 tgcctaccgg gttataaagc gaagtattat aaaatagatt taagtaaatg ataaataagg
     3661 aatattagtt agtaaggcca ttgagctttc aatattccgg atgtaacggt tgttgatcgg
     3721 atttaaaatt ttatttacgg agaaacgatg acaatgaatg aaaaatatac tgcccatctt
     3781 aaagaaaatt acggcaaaaa tattctgcca ttgtccgttc ttgaaaatga acggatgatc
     3841 gccggaagtc cgaacgaaat tttatatttt tacaggaaag acattcccat cgggaaactc
     3901 aaagaaagca tcatcaaaac cattgagcat tataatctct tttccagtcg tctgatcgcg
     3961 atcggtgaaa acaagtttgc attgcaatat tgtacggacg gtgccgtgtt gaatgagttg
     4021 ccgccgcttg acgccgtttc ccgtgatctc agtcttgaag acattaaaaa gatgatggtc
     4081 cacgttaaaa cattgccggg cgaaccgctt tttgctatta cgggcattcc ggtcaaagac
     4141 gggattttgg ccggcataag ctgttcgcat acggtggcag acggcatttc catgcttctt
     4201 ttcctctatg cctggatgtg tataactgaa ggcaggcctt ttctgcctcc atcttctcag
     4261 agattattca aagggctgcc tgttgattct gataaaatcc acagagtatt tctcccatcg
     4321 ctttccgaac tcagcagtga aattaaagat aaggccggac attatgaaaa agatgaaaaa
     4381 ttatatacca gaagggaatt cttttcagat gaatttcttg cggatatgaa acgcacagcc
     4441 aaacaggaaa acgagacctg ttccatttcc agtaatcaaa ttattatttc atttttattg
     4501 aaaaaatatc acgatcacat cctgcgcgat accgacaagg tgagattaag aaacgggatc
     4561 aatctgcggg atctgcatcc agatatcgac cctctgcatc tcggcaatgc gacgtttacc
     4621 agcatgacgg aatttacgag aaaggaaatt catgccatgt ccattgccca aatcgccaat
     4681 cgcctcaaag caaccattgc cggatacaga aatgaaaaat ttgccaggga attatcctat
     4741 ttgaccgaat acggaataga gttcaatgct gaggttctca gacaacatca caaggcttat
     4801 aatagtgaga cggacatcaa ctcgacaaat ctgactcatt tgagcgacct ggaatccctt
     4861 ggtctgagtt caaatatcgg gacgatttta gacgtgagtt cggttgttca tgcgggcttc
     4921 gttatcctga aagaaaagaa cggacagatc tttgcggatg tcacaagccg ttacccgttt
     4981 gtgcaggagt gatgctgcgg atgcaatcct ccagccgctg tttgcttttg agcagatcct
     5041 gtggtttgac ttccgtcgtg atggttgagg cgtatccctt ggcaatcagt cgtttgaaaa
     5101 tcgttttgta atcaatggag ccgtcaccca gggcgaggtg aaggtcgtca cttactgttt
     5161 ttcctccatg gttatcatgg gcgtggacgt gcgcgatgcg aggaaaatgt tcttcaataa
     5221 agcgaaaaga tgtgttttgc cgggaaagca actggccgtg tccgatatcg agcgtcattt
     5281 tcagatcggg aatggcgtca aaggcggggg cgaaactgtc ggcgcgttcg ctcaaatttt
     5341 ctatgcagac ggtaatgccg aaatcattgc cgtagtccac gatacgggaa agaatgttca
     5401 gcttgtcgcg gatcagatca tgaggcagac ggcgtttgtc aatccagaag tgaatggtcg
     5461 ctttatgaat gtcaagtttt tgagataatt ccatcaaggt gacgatacgg gggagaaacg
     5521 tattgcgaag aattgccaca tcaaaggggt tgtcttcatt ggggtaatgc gccaaaaacg
     5581 ttatgccgtt ccggttcagg atgttccgca gttgaggaag ccagcccgaa accacagcgg
     5641 gatcatccag cgatatttca atataggggc aatgtagttt gctgacggtg atggcttcat
     5701 caaggttgtg ggcccgggcg ccgataatca tcataaatac tcgattgcag aatgctaaaa
     5761 agaaagcttt agagacgtct tttctgtttt aattgtgtca taatctgctg taattcagga
     5821 agcttcagtg tggccgcttt ttcaccttcg agaatcgctt cattgaattt ttccatttcg
     5881 gtggaactga tatgtttcat gttcggacgg atgacgacgt cggcatgttt gatctgatat
     5941 tcggcgattt tggaatacat gatatccaca gattttttga tcgaatcgaa catgccgtcc
     6001 ggaacggttc tttggagtcc tccggaaata tccacggcaa tgacgatatc cgcaccgtta
     6061 cgacgggcaa cattgaccgc cacggggctg accacaccgc cgtccacgta agtgttattc
     6121 ccgattttca gcggctggaa aacagcggga acggagcaac tggcgcgaac ggccattccc
     6181 gtattgccgc gggcaaaaac cgtttcttca ccggtggcaa tgttggtggc cacggcataa
     6241 aaaggaatct taagtttttc tatcggtgtt tttttgacat acgtattgat aaaattttcc
     6301 agtttgtcgc cgatgataag accgccgctc catattttcc aatcgtagtc aataatctta
     6361 tccttatcca ttttcagggc gatttcctgc aattcgaaag cggatttgcc tgacgcatag
     6421 agactgccga caacactgcc ggcgcttgtg cccacgacca tgtgcacggg gatttgctgt
     6481 gcttcaagaa ccttcagcac accgatatgc gcgaatcctt tggaaccgcc gccgcccagc
     6541 accagggcaa ttttggcggg tttggcgggg ggagggaaga gggtattgat cagagaacag
     6601 gcgaccagtg acgctacggc aaggatgagc gccaagcaaa gaatggtcct gcgaaacatt
     6661 cccgtggaac ggccgtgtaa tggatgcctg tgattcatca tttattgtga tcctcggagc
     6721 ctcaacgttt tattttctta caacgtcttt cggtattttg tcaacccggg gtgttacaga
     6781 aatcagacgg gggatgatat ttgtgatttg tatttctttc aagacatgtt atatataaac
     6841 atctcataca ttcaaacggc aatgcgtcga aaaaaaaagc gtatccggca tcattgatcc
     6901 gctttcttat gaatatagtt ttaatcgagc tgttgttaca ccatatcaaa acgagagggc
     6961 tgatacgatg caaacagttg agaaaactag ttataagaaa gatgaaaaaa aggaatttac
     7021 tccggatgag ttcgccaagg caaaaacgac ctgcgaattc gttattgaac tcatcaaggc
     7081 catttcccgc agcggctatt atgacagcca ccacccggtt tcccaggaag tcaaaaaagg
     7141 cctctacatc gcttttaaaa agacactggg cgattcctcc gaaatcatgc tttcctgtca
     7201 tgagtacgag gataaggtgg acatccatat cagcggcatt ctcaatgaac cgttcaatat
     7261 cagaaaatta acacaatcca gcacatcgga tctgtttgtg ccgaaattga aagactactt
     7321 tgagagaaaa agtctcaaca gttttgtcat caaaaaaaat attacagccg agcacttcga
     7381 gtcttttatc gatgtgatga gcgagccgat tgcggaatcc gcggacacat ccaaactggg
     7441 cgaatacctc acaaaatctc ttgttgacag ggggatcacc gaagtatcga ctattttcaa
     7501 aacagatatt gtcctttcca gagggaaact tccctggcgt gtatccatca tcttgcgccg
     7561 tctggccaag gatctgaacg tggtgccgat gttccgtttt gccaccgaag ataaaatgaa
     7621 ggtaatcaaa aagcagattg tggaagatat catccgtccc ctcacgaata atgatttact
     7681 aaaagacctg attgtcaact gcgatattgt cgtggatcat gtctcccact tcatggaggt
     7741 tgatgagttg gaaaaactga taatcagttc attgcaatct gatgccgtcg tgccgatttc
     7801 cacgacagta tttgatattt accgggataa cctgatggac ggggtttcgc aggaagagaa
     7861 tgaacagcgg ctcgaaagaa atgtttatct ggctaaagtt ctcaacatcg cggcagaaag
     7921 aatgattgcg gagaaaatgc ccgatataaa aaatttattt gaacaattgt tcgaatgtaa
     7981 aattatcgat tcagatatgc ttccggcgga catgcgtatt gacagggaaa gcgctcaact
     8041 ggccgatgaa atattatcgc aaacagagaa ttacctggaa aacatctcga aggcgtcctc
     8101 cattgaggag gcggaaagta ttgtcttaac ggtcaagagg gtcatccccg cgttcgtcaa
     8161 tgccggtgag tggaaaatca tcgcgcggat tgtgcaggct ctaaacgatt tgtccgttca
     8221 tcatgaagac atgattcagg aatcggaatt tcttaaaggc atgcctgatt ccatctggga
     8281 aggcatggaa gaaaggataa ccgatgaata catccagggg gatgcaaatt tacgaagcaa
     8341 aatcgacgag gttttgctgc tgtcgaacac gatgtgcatt aaaaccgtcg acctgatgat
     8401 cagtaaatcg caagagccga acattttgaa atgtgcagtg gagatattat cccaaaaggg
     8461 agaaacaggc aggggatggg cgatggatgt tctcggacag tcgaatcatc cgttatccgt
     8521 gttgaatgcc gctttgctcg tcatcatgca ggtcggacac aaagaggata gcaaaacggt
     8581 acggaaattt cttaaacaca ccaatgcaag catcagagcg agagccctgg cggccatggc
     8641 aaaaatcaat caacaggaca ccgccgccgt cattatagac gctttgaacg atgaagagga
     8701 aaaggtcaga catcaggctt tgagtctgat cgagcatgaa ctgcctgttt ctgtagagat
     8761 gctccaaaaa atagtatcgt tcttaaaaga gagaatctca aagaagaata tcaatgcaaa
     8821 tgatgccggg ttggtgtgcg gtttattaaa agcggccggt catcttggag aggtttctga
     8881 tcgcgaaaca ctggagaatg agatcataga gatggccgcc gaattgctga acgaaaaaaa
     8941 agggattcta aaattcatga aagcggagat tccccgggaa aaacaggata tcctgtgcgc
     9001 ctgtgccgta tcgctgggaa aatgcggagg gataaaatcg aaggagtttt taaaaacgct
     9061 ttcccgcaac aataatgacg cggtagcaaa cacggcgaaa gacgctatgg acatcctgga
     9121 tcaaaaagcc gcccgtcagt gaaggacgtt gcgaacaggg cgcacttctt tttgcataat
     9181 ttcatgtcag atgtgcaccg gtatgattgc gcagcagttc agcaaaatca tcggctttga
     9241 ccggtttgct gaaataaaag ccctgcattt catcgcaaac gtgctcccgc aaataatttt
     9301 cctgctcgag ggtttccact ccttcggcaa cgacggtcag gctgagggtt ttccccattg
     9361 tgatgatcgc ttcggtgata gccttatcct cggaatcctt cggaagattc cggatgaaag
     9421 acctgtccac tttcagtgta tcgatcgggt aatgtttgat ttgtgccaga gaggaatagc
     9481 ccgtgccgaa atcgtcgatt gccagccgga cgcccatgtc cttgattttg gtcagcagtt
     9541 gaatgagctt tgcgggatta tgcatgacca tactttccgt gatttccaac tccagaagtt
     9601 gaggcggcat gccggattcg tttaatgcca tccggatatc gtttagcagg ttttcgtccg
     9661 agagttgacg cagcgataag ttgacggcca tgcagatggg cggcagattc tgcttctgcc
     9721 acgccatgtt ttgtgcgcag gctgttttaa gcacccatct gccgatggga acgatcaaac
     9781 cggtttcttc ggcgagggga atgaattgcg taggtgtcac ctggcccaga taaggattgt
     9841 tccagcgaag cagcgcttcc acaccggtga tttggtttga tttgaaatcc aatctcgcct
     9901 gatacgtcag ataaaattcg tcgcgctcca gcgcccgtcg aagattggtt tcaatcgaga
     9961 gacgttcatt cgattgcgtt ttgatgtctt tggaatagta ttgaaaattg tttttacctt
    10021 cctctttcgc aaaatacatg gcgatgtcgg cgtttttcat cagggactgc tcgtcttgtc
    10081 cgtccttggg aaagatgctg attccaatgc tggcggtgac gcggcattct tccgacatga
    10141 tcaccatggg cttgatggcg gcggaaagaa ttttgtgcgc cactttcgac acctgggcga
    10201 agtcgctgaa atcttcgatg agaatcacga attcatcacc gccaagacgg ccgacgacat
    10261 cgacggctct taacgaatgc cggaagcgtc tggcgatttc ctgtaaaaga aggtcgccgg
    10321 catcgtgacc gagcgtgtca ttgatgattt tgaaacggtc gagatcaata aagaaaacgg
    10381 ccagctgttt tttatggcgc ttggcggaga gaatcgcctg gttcagcaac tggttgaaca
    10441 ttaaacggtt cggcaatccg gtcaacgcat catgggttgc catgtaattt aattcctgtt
    10501 cgatccgctt gcgttcagaa atatcccgga tgactccttt aaaacctgca ggatgtccat
    10561 gggaatcttt ttttagcgaa acagacgctt caatgtatct ttttgtgctg tctttccgga
    10621 taatcagcca gtcaaagcct tccgtcggtt ggccggtatt gaaaacctta ttgaatacct
    10681 gaaaaacttt tttcgagttt tcttcatctg aaaattttgt ataatttgtt cccctcagtt
    10741 cctccttgga gcaaccggtc atccggcaca aggcatcgtt gaagaatgta aagtttccgg
    10801 caagatcaac ttcaaaatac gcctcctgaa tattttccag aatgtttctg tatttttctt
    10861 cgctttcacg gagagcttca agcgccgcct ccttttggaa ttcggcctga atgcgatcgg
    10921 tgacgtcgcg ggtaaaaccc tgaaaccctg tcggctttcc cgaggaatct ttctttaaat
    10981 agatagaggc ctcgacatat cgtctggttt tattcttccg gataatgaac cagtcgaatt
    11041 ctttgaccgg catcccggtt cggaagattc cgttaaacgt gcggaaaacg tcttgcgcac
    11101 tgtctttatc ggtgtattgc ctgtaattca ttcccaaggt ctcttctctg gagtaaccga
    11161 gcagcgaaca gagcgaatga ttgaagaaag taaaattgcc ggcaagatcc acctcgaaga
    11221 agccttcttc gatattttcc agaatgcttc ggtacttctc ctcgctttgg cgcagggctt
    11281 cttccgcctg tttgcgctgc gttctgtccc gcacgatacc gcggaaggca atgattcttc
    11341 ccgcagagtt gttgattaac gatatggatg tttccagaaa ttttctttga ccgtctttgg
    11401 tgatgatttg atattcaaaa tccttctccg gtatgccggt cttaaaaacg tgattgtatt
    11461 ttgcatagat tttcgcggca ttttcttcat ccatgacatc tgtataattc attttcagta
    11521 attctatgct ggtgaaaccg tgcattttgc tgatggcatc attaaagaag atgaaattcc
    11581 ccttgagatc catttcgaca tagccgtctt cgatgttttc gatgatggtt cggtattttt
    11641 cttcgctttg gcgcagggct tcttccgtca ttttccgttc ggtaatgtcc atgaaacttc
    11701 ccagtgtcgc cggttgccct ttataaacga tgggcgtaac ggtttccaga atccatctga
    11761 gttgattgtc tttgttgaac agccggaatt catagggctc gaaaatttct ttttttaaac
    11821 atttgacggc atgttgccgg acattttcct ggtcctccgg atacacacga tcgagggggg
    11881 atgccccgat cagatcgtca tctgtaaaac cggtaattat ttgaaaaaga ttgctggtgt
    11941 atacgaattt ccgattctgc acgatgtaga ttccgacacc ggccttcgcg atgatttctt
    12001 ccgacaggag agaaaattcg tttgggtacg gggatgtttt tttgtttttg ctttttatcg
    12061 gatgttttgt cggcatcgtt attttacctc tatgtatgga tagaaccgcg gttccatcca
    12121 tatgcctgtt tgggaaatta tcaaggtcgt cctgtggaga aatcggttat gaattaatct
    12181 gtcttggacc gctccacgaa ggagccggca gcggcaatgc attattccct gaccgctatg
    12241 gcttcgattt ctatcggtac gtctctcggc aatacgctga cttccactgt tgaacgggcc
    12301 ggaggatcct tggggaaaaa tccggcatag acttcattga ccgccgggaa atctttaatg
    12361 tctttcaaaa aaattgttgt ttttacgata tgggacaaat ccaggccggt tttttttaaa
    12421 agggcctgca tgtttgtcag aatctgtccg gtggcgattt tgatgtctgt gatgattttc
    12481 ccggttgccg gatcaacagg aagttgcccg gaaataaaaa taatgtcatt ggcttccacc
    12541 gctgcggaat aaggaccaga gacgggacct gtatcggacg aaataatttt ctttttcatc
    12601 atggcatcaa ccatccggca atattacgtt gtctgtaaca ttctttctat ggagagcctg
    12661 gctttgtcca taatatcagg ggcaaccgta atctcatacg aaagggtttc caaagagtgt
    12721 aaaacttttt ccaaagtgat tttcttcata tccgcacaga tcgccttgtc cgatgcagga
    12781 taaaagaatt tatccggatt ttctttcttt aatcggtgaa ggatgcctgt ttccgtcccg
    12841 atgataaatt ctttgtccgg agagttttgg gcgtggcggc tcataccttc ggtggaggtg
    12901 acgatatccg ctaaagccgt gatttcgggc ttgcactcgg gatggacaat gactttggcc
    12961 tgcggatgaa ggcgttttgc ctgttggatg tgctccggta aaatatcggt atgtaccgga
    13021 cagtatcctt cccagataat aaaatgacat tgtgcgttcg cggaaacgta acgggccagg
    13081 ttttgatccg gaacgaagat gatatcccgg tgatccgggg caattttccg cgtcatgggc
    13141 aacgcgttgg cggacgtgca gcaataatcg cacaacgctt taacacgggc ggaggaattc
    13201 acataacaca tggtgacggc ccccggatgt ctggattgaa gggctctgag gccttccggt
    13261 tcgatcatgt cggccatcgc acaccctgcg tccttgtccg gcagcaatac ggttttttgc
    13321 ggcgataata ttttggcggt ttccgccata aagcggacac cgcagaagac aatgatgtcg
    13381 gcgtcggttt ttgccgcttc catgctcagc ccgagggagt ctcccacgaa atcggcaatg
    13441 tcctgaattt caggcagctc ataattgtgc gccagaataa ttgcattttt ttcctgttta
    13501 agtttattga ttctgtcgac aatggtcatg agtgctcctg ctgtttatcg cgtctgtata
    13561 aggtccctct gctttgacgg cgattaaatc aaaacaaaag atttgtcaag aattaagagc
    13621 ccccgctttt tcaggaatcg atagccgtgc tgcttaaaaa tgcaaaagcg cacaggatac
    13681 caagcgtcat cacagcggat ggtgtgcaat cagaaaaaac ggttccgggt aaagatgacg
    13741 agtttatcgc cgggagacaa tttctggttg acgccggcaa ggtcgttcca gatcataatg
    13801 gctttgaccg acacattcag gtttttagcg atggtgtaaa tgctgtcgcc tttttgaacc
    13861 gtatatgtgg cgttttcctt ttcgatatgc cactgattca gcaagttctc gtagcgcccg
    13921 gaaaatccgt cggctgtccc tttgggaata agaacgctgt gacgaccggg gggaagatca
    13981 taacccttca aatgcggatt gagctccttg attattttaa aatacgtatt ggccgcagcg
    14041 gcgatgacct gtatcgggac gggattgtcg gctgagattt ccacccggtc aaattgcagc
    14101 ggaggataca gatcgtcttt acttaaatga aatccgtatt tttcgggatc agacatgata
    14161 atcttggccg ccagaattct aaagacgtat ctttgcgttt cctgattcag atgaagcaga
    14221 taatagttgt ttgtttgctg cattaaaatt tcagttttca gtccgtcttc acccatgttg
    14281 taggcagcag ccgccagtgt ccaggaattg aaaatcgaat aaagatcttt caaataggca
    14341 agggccgcat ccgtggcgat gaaaaagttg cggcgctcgt cgatatcaga attgacggtt
    14401 atattgtacc ttttcccggt gctttcaata aattgccaga aaccgaccgc tcctttgtgg
    14461 gatgccgcca gcggtttcag agcgctttcc gcaatcacga tatatttcag atcgtcggga
    14521 aggcggtttt ctctcaaaac catttcaatg tgcggaaaat atcgataagc gcgtttgagc
    14581 cacaggatga catcatcgct gttctgcagg gagacaagca tctctctttc cagtctttct
    14641 ctgacgtcgt tttcctttaa aggcactttt tcgttgcaga aggtcagggg ctctttgact
    14701 ttcagtgcgt caatcagaga ttgtccggca gatttttgcc agccgatctg agcgtttgcc
    14761 gtttcttccg ccgcaatgac caaggcgatg gtcatgatga tgagcgtaaa aaatttataa
    14821 atcttattca tggccttatc tccttgtttg atgcggggta tcacccggag gcaaaaaata
    14881 tttattcaat ggttaaacgg gaaaaagaaa catattcatt gaggtgatca taaagcaagt
    14941 tgtttcctct tgtcaacgtt tcatgttggt gttatgaaaa aaggcatgaa aaaaaagatc
    15001 aaacccgaag cccattcgct gttttccgga aaagatctgc aaaacatgga acgtttgcga
    15061 ctgaatctca acgatgtcga aaggcagttg accttgcacc ggaaggggcc gttattccta
    15121 aaattaaaca gaccgtgcac agatcatgac gggatcgttt ccttttcttc ggcacggcgc
    15181 cgggcattaa tctctttcta tgaacgggaa tcgtgtaaat acgacatcat caaatttgtt
    15241 ccggcatcgg gtgccgccag ccgcatgttc agcgaatggt tttcagcatc cgagaagggg
    15301 ggcttcggat cgaaagccct cgacagatta tttgttgcaa atttaaaaaa atttcctttt
    15361 ttcgatctga tccggcaaaa cgatccgggt cggaaactgc ttgccgaaaa ggatgtcggc
    15421 cgtttgctga aatttatttt gtcttccggg gggatgaatt tcgggaagcg tcccaaagcg
    15481 ctgattccct ttcaccgtta ttctgcacgg gaaattcgca cgcctctgga ggagcacgtg
    15541 tgcgaagcag cgttgtacac cctcaaccga ggagtatgtc atattcattt caccctgtcc
    15601 cccggataca aatccggagt ggcccggtat ctgaaaaagg ttatcgccgg atatgaaaaa
    15661 agaaaccgga tcagatataa tatctctctg tccgtacagt cggcgtcaac gaacacgata
    15721 gccgtcgata aaaacaatct tccgttcaga gatgaccaag ggcgtattgt gtttcgtccc
    15781 ggagggcacg gggctttgct gaaaaacctg aagcacctgg atgcggattt catttttgtc
    15841 cgaaatatcg ataatgcggc cccggaaacg ttgtgggaaa aaatcattcc ccacagaaaa
    15901 atgctgggcg gtctggcgat ccggatgcag gaggaaatat tttcgcacat acggaaactg
    15961 aaaaataaca aaccggatgt ttcgcagatc aggaatattg caaatttctg ttcggagcaa
    16021 ttaaatattg tttttgccga aggctttgac cgacaaccgg catcggaaaa gatacggact
    16081 cttctttctt gtttgaacaa accgctacgg gtttgcggga tggtccgaaa cgaaaatcag
    16141 ccgggcggcg gtcctttctg ggtggaagaa aaagacggga cgcagaccct tcagattgtt
    16201 gaaagcgcgc atgtggatac gaaacagacg gatcaggctg ccatctggtt tggcgcggaa
    16261 tatttcaatc cggttgatat ggtctgctgc atccgggatt accgggggag ggttttcgat
    16321 ctctccgact atgtcaacaa cgatacgtat ctgattacgt cgaaaagcga gaaaggacgc
    16381 caactcaagg cgttggaact gcccggttta tggaacggtt ccatggcgta ctggaatacg
    16441 gtgtttgtga aattaccgat cgttgttttt aatccggtca aaacggttta tgatcttttg
    16501 cgtcccgggc attcggtccg atagaatgtg tttcggatcc tacacgttta aagaatccat
    16561 cgctgacaat cttcttttga tgctgcggca gccgtgacga tttccgactg gttgtcccgg
    16621 acgcagatga cctcctgcag ggcatccggc ggcgcatcgc tgtaatgcac acataacgca
    16681 gcggcgatct gtggatcgca taggctgtcc cggggaacaa caacgagggg tccgggaaaa
    16741 tgagccatat tgaacaggac gtcgccggag gcggcaagat gtttcaaagc ctcattgtcg
    16801 ccgctgtttc gtccgacaat gattttgcat gtatcatgaa tgcggaaatg acggccgtgt
    16861 ttcagtaatt cataatcacg gatttccggt tcggtctgat gggcaaacaa atcgcgcagg
    16921 cgacgggtaa acacgggatc ggtcagcagg catccgccgg ccggaggagc ataacttgtg
    16981 atgccgaaat ccgcggccag gatgatttgc tgttttcggc cgcgtccctg aatggcaagt
    17041 aactgtgagc ggtcgatcag attttctttt tctgcttttg tcggttccag aagacgagcg
    17101 cttaagggac gtaaaataaa gtcccggtac cctgaatttt tggcgacaat attcagggat
    17161 tgcttattct gcgacatcgg gcgctgtccc agaacttctc cggtaacaat gaaatcgctc
    17221 ccggtttgat ccattttccg tccggcaatg ttcagcatca acgtgtggca gtcgatgcag
    17281 ggattcatgt ttttgccgta accgtaacgg ggcgaccgga gcatttttaa atgctcagac
    17341 gtgatatcca tgacttccag cggcagattg aactggcggc ttgccgccag cgctttttgt
    17401 gcattgaaaa acggtgtggt gagggtcaga cagagaacgt cgatggtttg tctgcggact
    17461 aattccgcgg ccaggatgct gtcgaggccg ccggaaaaaa gagcaatcgc tttggtcata
    17521 aaattccaat cctttaacaa tacattaccg gttgtttagc tgccgctgtc atgaaaaaca
    17581 attttgagaa gaaaaacaag aacaaaattg atgcgtcgag cgtttgccgg ttgagcggat
    17641 cgacgaagca accgcagctt cgaagcagat gttttaaaag cttcaatgat ttacgggcat
    17701 gtccgatttt tcgagcaggg gattaatacc gggcaatcct ggccgaccag acttcctgga
    17761 gtaatccgcg cagtcccgga tcgatatcaa aattgacgac atataaatcc ggattgattc
    17821 tggtgccgct gaatttcaga gcataaggag cctgattttt ttccagcccg gaaaatgcgt
    17881 gcgtgccgta agtaaaagga atatccaggc cgctcaagac gttgcccatg atgtctttgg
    17941 tggaatcgct gttgcttaca aagatggttt cattgccggc ctgtttcagg gcgtttataa
    18001 attgttgaga gagagactgg gaatatatga catatttatt gtttcccttg gtgacgagaa
    18061 cgtccgcttt taaggaaaga ttaaatccgt cctttaccgt gtcaaagatt tgaatttcga
    18121 catctttttc tgcactgagt cccaggtggg aaacaagcgc gtgggaaaat tccttggcat
    18181 tggtcgccgg aaagacggga atcggcggca gggaataaag ttcctcgggc ttatccgcaa
    18241 tgcctgtttc ctcggagaac tcggttatga ttaagccgtt tttctgggca taatttttaa
    18301 tggatttggg cagcagaaca ttattttcgt aaatctgccg cagtccctgg atgactgatt
    18361 tcgattgctg tcctgggatg ggtttgacga tcaaccaatc caccatgact tccgcgggcg
    18421 gcaaggcgcc gaaggttaga ggcttgtcgc ttttgagaat gctgtacttg ttcgttacac
    18481 tgaaaacttt tttcagaata gtaatggtat catcagtatt gctgattttg accaaatgat
    18541 aatttttcca gttgttggaa atcatctttt tcaaattact gtgagcgcgg ttttccaaat
    18601 cgagaaaaac ggtcgtgttg tcgtcaaatt caatgagcgg aatcttggag caatcgatgg
    18661 taacctgtcc ggcctggggg atgggaagat aataattgcc cgttgttgtg accgttccat
    18721 tcatctgcgt gatgatctgc tttagcaagg caagacgggc ttcaaacggc atcattttct
    18781 tgtctttgat ctcaatgatc cggtcttcct gaacgtattg ttcggcggtg tcgcttatgg
    18841 ccggcttgcg ggttgccggc gtaaggtcgt cagggctttg aacaaaaacg gttttcccgg
    18901 ggagttttag catggtgccg acatagattt tgttgacatt tttgatggtc ggattcaaaa
    18961 ctttaatgct tcgcaccacc tgcgggatgg atgtttccga gaccttgaat tcgcggcgga
    19021 tgatcttgta taaggtgtcc cctcgtttta ctttataata aaattcaccg gcagatacgg
    19081 ttttcggagc ggctgcagga gctttcacat ttgtccggtc gtttttcatt ttcattggtt
    19141 tttccggaag aaagatggat tgtccgactt cgaggttttc aatatttgct atgtggggat
    19201 tcatatcctt gatgagacga tacgtgtaag ggatgtcttt ttctgaaatg ccgggaatgc
    19261 ttttgatgat attgattaaa acatcccctt cctttaccgt gtaggtgtgc aggttttttc
    19321 tggaaatggc ggttttcttc agttgaatct gcgccgtatc ttcccgcgaa gcttcgacaa
    19381 tgttccaagc caaggttgtt gaagctaaaa cgatcaacag agccaaaacg taccgcttaa
    19441 acatcagttc cttccccctg gttgagatat tcagtgtata aatattgtat gaaaatactt
    19501 taattggcaa taaaagtcaa cttattctat ggctttgaga gaaagcggac ggtttcttcc
    19561 gaaaaaaagc tggatggctg accgaaaaat acattcgggc tgacgagacc tgaacaaaaa
    19621 gttaaatcac ttttttcatg gcggcaatgg ccacctctat ctggtcatcc gaaggttcgc
    19681 gggtggtaat tctctgcagc atgagccccg gccagcttaa gatctgcacc agtctggaat
    19741 gtttgtattt cccggtaaaa cgaatcgctt cataggatat tccggatacg acaggaagca
    19801 gcaggatttt gtagaggacc cggtgcagaa aatcgggacg tcccagcagg gaaaaaacca
    19861 gaatggaaac aatcatcaca aaaagaatga aacttgttcc gcagcgcgga tgacgggtgg
    19921 agaaattctt gatgttttca acggtcaggt cttgcccgtt ttcccacgcg aatacggttt
    19981 tgtgttcggc gccgtgatac atgtagacgc gacgcatatc cttcatcagc aaagtggcgg
    20041 ccagaaaaca aagaaacatt cccaggcgga cacatccctc aagaatattt aaaagcagaa
    20101 gattgtcgat atagaacttc attttagtga agcaatatgc cggaacaacc acaaagaaaa
    20161 aaatggcaat cgaaaaactg atcatcagag aaagatacat ttcccagtct ttgggttttc
    20221 gttcctcttc cgaaagggca acatccgcag agaacatcag cgctttgaaa ccgacgatca
    20281 tcatttcaaa caacgtgatg gttccgcgga taaacatcca tccgaggact ttataacgtt
    20341 tggtcagggg aatatattcg cgctcctgaa aaacaatttc attatccggt cttcggacgg
    20401 ccgccgctat cttatcgccg tggcgcatca tgacgccttc gatcagcgcc tggcctccgg
    20461 cgatatcttc cggttgtggt ttggggggca tataaccttc actgcaaatt tttgcgtctt
    20521 ctttatagca tttaccgccc ggtgtcaaat tatgattttg aaattcatgg ttgcaagata
    20581 aacgtccgat gatataatgc cgtaacttgc cgattaaaag gagatggaaa atggcgcaaa
    20641 tgagttcaac ggatttattt attcgagaat atcaggatcg cttcgagaag aaaatcagag
    20701 aaaacgagat tgcttctctg gaacactgga aagcccagtt ggacaaagtg attgccatga
    20761 gacccgacgg tgtggcgtcc atgcagttac agataacaaa aatatcggaa atgatgtcca
    20821 atcggatcaa aacgctgaaa aaggaatagc atggacagta tcgccaattt cttgtttgag
    20881 gtggggatgc tcagcagaac gccgcgcagc ggttatcagt ttctgggcag cggtcaggag
    20941 tcggtggccg aacacattct ccgcacggtc tttgtgggat atacgctttg caagatggat
    21001 tcgtctctcg atgagatgcg cgtcctgaaa atgtgtattt ttcacgatct gccggaagcc
    21061 cgaaccggcg atatgaatta cgtcaataaa aagtacgtca aggtggatga ggaaaaagca
    21121 atccgcgaat tggcggaaag tctttctttc ggcgcggata tcagatcggc aatcgacgag
    21181 tttaatcaga aaaaaacaaa ggaagccctg atcgccaggg atgccgatca aattgccctg
    21241 attttacagt tgaaagaatg cggcgatctg ggcaataaat acgcggaaga atggataggt
    21301 tttgccgtgc agagattaaa cacggaaaac gggaaaaaac tttctgaaaa aattaccggg
    21361 acggattctt ctctgtggtg gtttaaagaa aagggcgact ggtgggtgaa cggtaataat
    21421 ccatgaagaa aacatcgccg gcacataaaa cgattctatg attcataaaa aacatcgtgt
    21481 gtcctttttc ctcctcgtcc tgtcggtcct caacctcatc tcctgtttcg gcctcggacc
    21541 gggcggcacc gtgatttcaa aaccggatca gatcacgcga acttttgaaa cgaaagaaac
    21601 agcggcgctt agagcggttg cccgtgtgtt cagtgaaaaa aagatgggcg ccgacacgaa
    21661 aatagactat gaaaacaaac gggtcgattc cgattatgtg gtttcgggac agtggaggac
    21721 caaagctgcc gctgtagtcc gacaaatcaa ctggaaggaa tgcgaggtaa ccgtttctgt
    21781 gattacggaa aagcatgcgg aaaaaggctg ggaaatgcgc aggcttctgc agcatccaca
    21841 gtatgataat ttgttcagtg tgatcgagtt gaaaatttac gaggaaatgt caagagtcaa
    21901 ttaaaacatt cagaggaggg gaatatggcg gcaaaaaaca aagtatccgc gatgatggag
    21961 gagtttaaaa attttgcttt caagggaaat gttgtcgatc tggccatcgg tgtcatcatt
    22021 ggagctgcct tcggtaaaat tgtcgattcg ctggtgaagc atattatcat gccgctggtt
    22081 agtgttgtaa taccgggaga gcagggttat ctgggatgga agtggatcat caacggcaag
    22141 gaagtgcctt acgggttgtt catcggcgaa attgtaaatt ttctgatcgt ggccgtggcg
    22201 ctgtattttt ttattgtgaa atttctgggt atgatgatga agagcaaaga ggaagaagcc
    22261 gccggacctg caccattaac caaggatcag gaattgctga cggacattcg tgaactgctg
    22321 aaaaaataaa gcagggtcgc tccacaaaca gattttcacg tcgttacggc gaagggctcg
    22381 ggcaacttcg gttgcccgag cgtgatttat ctgagtgaat ttgagagatg atattattta
    22441 ttcgaacgac tttcgcggaa aaaattcgtt aaaaattctg tgtctttggg agacagatcg
    22501 aatttgaaaa tcgccttttc aatgagtttt tgcaggggca gattttcgct ttccaaactt
    22561 gctgaaatcc atttgattgc tttctgaaca ttctccgctt ccggcatgat cgtagccata
    22621 aaaaaccctc ctaaactgat ttggaaaaca catttttaaa atgatgattc catgcatttt
    22681 taattcatat actataaaat aacacagaaa ttaatttaaa tttttggaat tttgatttat
    22741 attttcaaaa tgaatgaaaa aaataacaaa atccgctaaa ttgctttttg aggagattgc
    22801 tgtgaaaaag tcatttggag ctaaaacgtt gatttatccg acccctgtat ggttgatcgg
    22861 aacgtatgat caagaaggca ggccgaatgc tgcgaccgtt gcctggtgcg gtgtctgcag
    22921 ttctgatccg cccgcgttgg cgatttcact gagaaaatcg cgatatactt atgaaagtat
    22981 tcagcaccgc aaggcgttta cggtaaatat tgccacggcc gatcaggttg tagcggcgga
    23041 ttactgcggc atggtgtccg gtaagaaaac ggataaattt gcagcagcgg gcatgacggc
    23101 tgtccacagc aacattgtgg atgcgcctta tatcgcggaa tgtcccatgg tggttgaatg
    23161 caggttgacg gatcagatgg aagtcgggat tcatgtgcat ctgattggag aaatatgtga
    23221 cgttaaagtc gaaacaaccc tgctgacaga ggatcaattg cccgatatca tgaaaatcca
    23281 gcctttgata tatgctccgg aaaatcgttc ttatcatgga gtcggcggat atctgggaga
    23341 ggccttttcc attggcaaaa aaattatgaa atgaccattg ctaagttcaa tcacagtgtt
    23401 taacatatca aaaaagaaaa ggagatgacg catggcaaag tcgattaagg gaacaaagac
    23461 ggaaaaaaat ttactggctt cttttgccgg tgaggcacag gcaagaaatc gctatacttt
    23521 ttttgccagc gcagcaaaaa aagaagggta tgaacagatt tcaagaatat ttctggaaac
    23581 cgcggaaaat gaaaaggagc acgccaaggt attcttcaaa tatcttgaag gcggtgaagt
    23641 tgagatcaca gcctcttatc cggccggggt aatcggagat acgatcagta atcttaaagc
    23701 cgcggcggaa ggtgaaaata tggaatggac cgttttgtat tccgacgcgg ccaaggtggc
    23761 cagagaggaa ggatttccgg acgtggcccg ttcttttgaa cagatcgcca aagtggagat
    23821 gtttcacgag aacagatacc gaaagctggc agagaatatc gcaaagggcg aagtctttaa
    23881 aaagaagaca gcgacaaaat ggcattgcat caattgcggt tatgttttcg aaggagaaga
    23941 agcaccaaaa gaatgtcctg catgcaaaca cccgcaggcc tattatgaag tgctggctga
    24001 aaattactag tgagacaagc tgagggcgag tgaaacgaag cacgaagcgt aagtcgaact
    24061 atccctgtga gcgaagcgaa tgggggtctt gagacaagct gagggcgagt gaaacgaagc
    24121 acgaagcgta agtcgaacta tccctgtgag cgaagcgaat gggggtcttg agacaagctg
    24181 agggcgagtg aaacgaagca cgaagcgtaa gtcgaactat ccaattctga taaatcggaa
    24241 ttggaaacta tcccggcagc caagtaggaa ggggatcatg agccattaag gattttcagg
    24301 cgaacagaac gatagagttg taagaataaa acagcttcct gcgtttctga tgtctcaatg
    24361 caggggctgt tttttttatc aacgctcgtt cttcttgaca gttcatataa aatgccatag
    24421 attggcagcg tttttctgag gaatggagta ttttatgtcg accaagaaac agctaaggtc
    24481 agagagcatc aaggaaaaga agataaaaaa atcaacccgg gtattttacc tttcgctgat
    24541 tgttggcggc ggtgtagtct tgctcgtcat tttttttatt accatattca gcgcactgtt
    24601 cccccccgtc gatacggacg tcatgaccaa aaaggaaagg cgggttgtta aaatttattt
    24661 ttcggatcag caggagcgtt ttttgaaggc tgaaaagcgc catgtcatca aggaaagcga
    24721 cattgcgttg caggctcagg aaatcgtcaa ggctctgctt gacggttcca agacgggact
    24781 ggtgaatacc tttcccaaaa acgtttcgct ccgggatgtc aaaattaagg atcatgatgt
    24841 tgcgtatgtt aatttcagca aagatctgat caaggcgtat caaggcagtt ctgccgcgga
    24901 gatgaatacg atttattcgt taacgaattc catcacgcag aatattccgg aaatcaaaag
    24961 agtaaaaatt ctggctgacg gtaaggagct ttcctcgatt cagggccaca tttcaacagg
    25021 caagtttttt tcgccggatc tcgaactcat tatcccggag caatccccta acaattaatc
    25081 gacgcatatg gcatccaaat caaaaatagc ccggacgcgc aatatcggta ttgtcgcaca
    25141 cattgacgcc ggaaagacaa cggttacgga aagaatcctt tactataccg gcaaatccta
    25201 caagatgggc gaggtgcatg acggtgaagc ggtaatggac tggatgccgc aggagcagga
    25261 aagaggcatc accataacat cggccgtgac gacctgtgtc tggcgcaatc atgacattca
    25321 tgtcatcgat acaccggggc atgtggattt taccatagaa gtggaacgtt cattgcgcgt
    25381 gctggacggg gccgtggtcg tcttctgcgc ggtgggcggt gtcgaaccgc aatccgaaac
    25441 cgtctggcac caggctgaca aatacggtgt tccgaaaatc gcgtttatta acaaaatgga
    25501 ccgagtggga gccgattatt ttaatgtgat tggaatgatg cgggaacgtt tttcctctct
    25561 gcccgtcgcc attcaaattc ccgccggcag tgaagataac tttcgtggcg tgctggatct
    25621 gattaatcag aaaatgatcg tctggaacga ttcgacatcc gggtcggaaa tgtctaagca
    25681 ggacgtgccg tcggaatttg ccgatcacgt gaagttgcag cgcgataaaa tgatcgaaac
    25741 gcttgccgag gttgacgaca gccttgccga taagtacctg ggcggtgaag aaattaccat
    25801 tgctgagata gaagaggcgc tgagaaaagc gacgatatct ctgaaaattg ttccggtctt
    25861 gtgcggctca gccctgcgca acaaagggat acaacccgtt ctggatgcca ttatcaacta
    25921 tctgccgtct cctgaagata ttccgcccgt caaaggaatg aatccggtaa cgaaagaaga
    25981 agagatcaga cgaagttccg agaaggaccc tttggcagcg ctcgcgttca aaattatgat
    26041 ggacgaggga agaaagctca cctatctgag aatttattcc ggaaaaattc aggtcaataa
    26101 cgaaatctat aatccgataa aaaaacggcg ggaaaagata gcccgccttt tacgcatgca
    26161 cgccaacaaa cgtgaaagga tagactcggc ctccgcagga gatatcattg ccgttctggg
    26221 attgaaatcc actaccaccg gcgatacgct ttgcgcggag cagaagccga ttttactgga
    26281 actgattgaa ttttatgaac cggtcatcag tcaggccatt gaagccaaaa caccagccga
    26341 tcaggaaaaa ctgacggcgg cgctggataa gctggtggaa gaggatccga cgttgaaggt
    26401 gaaatacgaa gatgaaaccg cccagacgat catttccggc atgggggaac tgcatctgga
    26461 aattattgtt gaccgtttgc tgcgggaatt caactgccat gtgaatgtgg gccggcccag
    26521 ggttgtctac cgcgaaacca tccagaagaa agtggaaatc gaaggtatat ttgaaaaaga
    26581 acttggcgaa aagaagcatt tcggtcacgt ccggattgcc ctgtccccgc gcaagagagg
    26641 ttcgggaaat gacgtgatca taaaacttga cgatagcgtc attcctcagg atttccatga
    26701 agtcatcgag gagggcatcc gggaagcaat gatgaacggc gtgttaaatg gttaccctgt
    26761 catggatacg gaagtcgcga ttatcggcgg ttctttccgt gagggcgaat cttcagcgca
    26821 ggggtataaa atagccgctt caaccgcatt tcgcgacggc tgcggtcagg cggagcccat
    26881 catgcttgaa ccgattatga tggtcgatgt gataacgccc agcgagttca tgggagatgt
    26941 gatcggcgac attaatgccc ggaagggtga aattcaggcc gttaatccca aaggaacggt
    27001 cagcgaaatt aaggcgaagg ttcccttgaa ggccatgttc gggtattcca ccgatttgag
    27061 gtcctcgaca caggggaggg ccgttttctc catgatcttt tccgaataca acaagattta
    27121 agcgtaattc gtattttttt tgcactaaaa tcagcctatc tctttgagtt tccatcggaa
    27181 tagccacttt aaaaattatt tattattccc ttgaaaatta ctatatatag tggtactatc
    27241 cttttcaacc ccaatatata gcgctatttt ggatttattc ttatattatt tttttcatcg
    27301 gatggagtgt ttttatgaat cttaaaatta gaaaaagaga cgggcgactg gttaaattta
    27361 acgcggaaaa aattaccaat gccattgcca aggccggaaa tgccacaggc gattttgaca
    27421 tcaaagaggc gcgtaaatta acgatcaaag tcctgaatct ggccgagaaa ctcttcgaca
    27481 gcaaaatcct gtccgtagaa gaaattcagg atgttgttga agaagttctg ctgagctctc
    27541 cgtaccgcaa gacagcaaag gcgtacatca tttatcgaga tcagcactcc cgtttgagag
    27601 atatcgccaa tcgaatggaa gttgatctcg tcgatcaata cctgaagaag cttgactgga
    27661 agatcaatga aaacagcaac atggattact ccctgcaggg attgaataat tatatttctt
    27721 cggaagtcag caaggtttac tggctcaatg aaatttacac gccggaaatt cacaaagcgc
    27781 atacggaagg cgattttcac attcacgatt tgagtctgct gtccgtttat tgcgtggggt
    27841 gggatcttta tgatctgctg ctgcagggat ttcaaggcgt gtccggaaaa gtggagagca
    27901 aaccggcgaa tcacttgcgc agcgcgctgg gacaggtggt gaatttcttt tatacgttgc
    27961 agggcgaagc cgccggcgct caggcctttt ccaattttga taccttgctt gctccattca
    28021 tccgctacga taatttgtct atgaaagaaa taaaacaggc ccttcaggaa tttattttca
    28081 atattaatgt gccgacaagg gttggatttc aaacgccttt tacaaatgtc accctggatg
    28141 taacagtgcc gaaatactac gccgaccaga atgtaatcat aggcggaaag ccgcagaaag
    28201 agacctacag agaatttcag gaagaaatga atcttttcaa taaggctttt ttgcaggtga
    28261 tggcggaagg cgacgccaag gggagggtgt ttacatttcc aattcccacc tacaacgtta
    28321 ccaaggactt taactgggac gatcccaact tgcaggatct ctgggaaatg acagccaagt
    28381 acggcgttcc ctatttttcc aattttatta attcggacat gaatcctgaa gacgcccgca
    28441 gcatgtgctg ccgcctgaga atcgacaacc gcgagctgga aatgagaggc ggcggtttgt
    28501 tcggctccaa tcctctgacc ggatctatcg gtgtcattac gataaacatg ccgcggattg
    28561 cttatctttc aaaatccaaa aaggattttt tgacgcgtct ggagcatctg atggaactgg
    28621 cgaaggaaag tctggagacc aagagaaagg tgctggagaa gttcacgtac ggaaatcttt
    28681 atccctatac gaagtattat ctgcgcggag tgaaagaacg gttcggcgag tactggaaga
    28741 atcatttctc cacgatcgga ctggtcggca tgaacgaggc ttgtctgaat ctgctgggcg
    28801 tgaacgttgc gacggataag ggacaggtat tcaccaagca ggttttggat ttcatgcgaa
    28861 acaaattgat cagtttccag aaagaaaccg gtaataatta taatctggaa tcgacgccgg
    28921 cagagggtac gtcgtaccgg ttggcgaaaa ttgataaaga gaagttcacg gatattatta
    28981 cggccagcga aggcaaatcg aaaaaggcgg aaccgtttta caccaattcc acccagttgc
    29041 cggtggatta taccgacgat atctttgaag cgctggattt gcaggatgaa attcaggtca
    29101 aatataccgg cggcacggtg tttcacatat ttgccggcga aagaattgac gatcctcaat
    29161 ccgtcaagaa actggttcag aagatttgtt tcaattatca tctgccgtac atcaccttca
    29221 ctcccacatt cagcgtctgt ccgtctcatg gatacattaa aggcgaacag gagaagtgtc
    29281 ccacctgcgc ggatgattgt gaagtctatt cccgcgtcgt cggttactta aggccggtca
    29341 agcagtggaa taaaggcaag caggaagagt ttgacttgcg ccaggaattc aaattcaaat
    29401 aaacggatca tgagagtcta gaatcatcag acgcggcggc gcgtttgttt ttttaatcta
    29461 tgagaggcgt atgatgaaga tcggcggttt gcagagagtg tcccttattg attatccggg
    29521 gtatatttcc gcaattatat tcacccaggg atgtaatttc aaatgtccct attgccataa
    29581 tcccgaattg gtcaatcccg atttatttcg cacacctatc aaagtcagtg aggtgattga
    29641 ttttcttgaa tcgcgaaaag gaaaacttga cgcagtatcg atttgcgggg gcgaaccgac
    29701 cattcaggat aatgtgatct ctttcatcag gcagatcaaa aaaataggat ttgcggttaa
    29761 actggatacc aacggctctg tgccgaatgt tctcaaaacc ctcattgccg acaacatgct
    29821 ggatttcatc gccatggatg tcaaggcgcc tctagacaag tacagggaca ttgtcaaggc
    29881 caatgtgaat caggatgcta ttcaggaaag catccggctg gttttaaacg caaagatacc
    29941 ctatgaattc cgcacaacga ttgtggaatc tcagctcgat gaaaacgata tcttgcagat
    30001 cggaaaaatg atcaacggcg ccggtcatta cgtgctgcag aattttatgg cgaagaagac
    30061 actggataaa aagttcgcga aggaaaaatc tttttcggaa gagaaactgc gaaaaattaa
    30121 aaaacaactt gagcagcaga ttccttctgt aaccatccgg tgatacatta tcttacagac
    30181 acttcctgtc tgtctattta tatacgtaat gtaaaacagg tacttgtagc cgatatgtca
    30241 agttctgtat cagctgccgc tcagattaaa gccaggctgt cggcaagagc cattccggaa
    30301 cgcgtcgggc aaagaaaacc gttttttaac tctatgagct tgtttttcat taaagcatcc
    30361 ataatagatt ttttttcagt catcaaatcg gtgccgtatt gatctttgaa gcgctttaga
    30421 tcgatgccgg atttgcagcg tagccccaga aacagagctt ccagccgcag ttgattgacg
    30481 gtaagtgttt ctgatgcttc aacgggcgtc ttgccgcggg ccatggcgtt taaataacgt
    30541 ttaacagatg ccttgttcca ccatcgttga ttattcagaa aagagttcgc ggccggtccc
    30601 agaccaagat agggaacgtg ctgccagtat ttctgattat gacgggatgt atatttgtct
    30661 tttcgggcaa agttggagac ttcgtaatgc cggtaaccga cattttccaa aatttcggat
    30721 gtcgttaaaa aaaatttcag ttccgcattg tcatccggaa gatgccatcc ggcggaaaga
    30781 tatgttttgt gaagcggtgt tttttcgtgg agggaaagct ggtagcagga gaaatgctca
    30841 ggcgccagta tcaacgcctg ttgcagaatt ttttcccatc cttgaagacc cagtccgtaa
    30901 acgccgtaaa tcaggtcgat acccagattt ctgaatccgg cttttctggc ggcgtttatc
    30961 gacgccatcg cttcctgagc cgaatgccgt cggccaaggc attttaaaat cttatcgtta
    31021 aaagattgaa ccccgatatt cagacgatta atccctgtgt cgcgcagcgc tttcaaatat
    31081 accggggaaa gatctcccgg attggcctcc agtgtgattt cagcgttttt atcaatgata
    31141 aaatatttgt ttaatgctgt gaatatttca tccaggtatt gcggggccag cagggatggc
    31201 gtgccgccgc cgatatagat ggaatcgaat gaagaaaaga cgttgcgata cagtttcatt
    31261 tccttttgga cggccgcgat atagtcagga atcaggtgaa gcgttttgac ggaatagaaa
    31321 tcgcaatagg cgcacttgct caggcaaaaa ggaacgtgaa tgtaaaggcc ggctttttcg
    31381 cttcgcataa gaatcaatca aagaaaagat cggttggtgt caagatctag tcggtatcgg
    31441 atttcagaac tgccagaaat gcactctgcg gtatgctgac cgaaccgatc atcttcatac
    31501 gtttttttcc cgctttttgt ttttcgagca gttttcgttt gcgcgtaatg tcgccgccat
    31561 agcatttggc tgtcacatcc ttgcgaaagg cgctgatggt ggtgcgcgca atgatttcac
    31621 cgccgatggc accctgaatg gcgatcttga acatctggcg aggaatctcc tccttcagtc
    31681 tttcacaggc cagcagagct cttgcgcgcg cccgatctcg atgagcgatc tgggagagcg
    31741 catcgacttt ttcaccgttg accaggatgt ccattaaaac cagattgctc tcgcggtaat
    31801 cgatgatgtc gtaatcaaaa gagccatagc cctgggtgac ggatttgaag cggtcataaa
    31861 aatcataaat cacttcggca agcggcatct cgtaggataa ttccacacgg ccgggaccgg
    31921 tataattcat gacagagttc acgccgcgtt tttccataca cagtttcatg accgctccca
    31981 ggtagcgttc cggaagcatc atggaggagc gaatgtaagg ctcctcacct ttgtcgattt
    32041 cgatcggatc gggataatgc gcgggattgt ccacgtaaat gacggttttg tctgtgagcg
    32101 aaaagcggta gcgtacgctg ggtacggaaa gaataatgga gaggttaaat tcccgttcca
    32161 gtctttcctg tacgatatca agatgaagca ggcctaaaaa tccacaacgg aagccctgtc
    32221 ccagggcggc cgaagaatct ttttcataaa taaaagatgc atcattgagc ttgtattttt
    32281 cgagcgagtc ggccagatcc tgataatcat cggacgcgat gggatagatg gaagcaaaaa
    32341 caaccggttt gacgtccttg aaaccgggca aaggctgaac gcatggcctg ttctgaagcg
    32401 tgatggtgtc accggtgcgc acatcagaaa cggttttgac gccggcgatg atgtagccga
    32461 cgtcaccggc ggaaagtttt tcgcgtttgg tgcggttcag cagaaaatgt cccacttcct
    32521 ccactttgta agtggcgccg ttggacataa atttgatgat gtcgccgctt ttgatggttc
    32581 cttcaaagac gcggcagtgg ataattgtgc cgcggaagga atcgtatttg gcgtcgaaaa
    32641 tcagagcggc cagaggattt gcggggcttc ccttgggcgg cgggatgcgc aggacaatcg
    32701 cttcgagaac ttcttcaatc ccggcgcctt ccttggcgga gcatttgata tgcgtgaagg
    32761 gatcaagccc caattccgaa tcaatttctt ccagtacgcg gtcaatgtcg gcagagggca
    32821 gatcgatttt attgatgacg ggaatgattt ccagatcatg ttccagcgcc atgtaaagat
    32881 tcgccagtgt ttgcgcctgc acgccctggg cggcatcgat cagaagcaaa acgccttcac
    32941 aggatgccag cgatcgggaa acctcatatg aaaaatcaac atgtcccggt gtatcgatca
    33001 gattgaggat gtacgttttt ccatctttgg cgcgataggg cagggagatg gcattgcttt
    33061 taatggtaat gccccgttcc cgttcgatat ccatattgtc cagaatctga tcctggaaat
    33121 ttctttcatt gcagacgccg gtaaattgaa tcagacggtc ggcaagcgtt gatttgccgt
    33181 gatcaatatg ggcgataatg ctgaaatttc ttatgttttc cataaagtat ttttaaaaaa
    33241 agccctccaa ggaatgctgg ggggcttttt ttgtcacgtg tcttaagttt ttatccgagt
    33301 ttcttcatca gttcatctgt aacttctttc acgccaacgc taccgtcaac ttcaataact
    33361 ttcacttttg ccttgtagaa attcacagcg gccagtgtgc cggtcttggt gtcgtagtag
    33421 atgccgtgac gtttgtcgat ggcggctgta tcctgatcgt cggcgcgggt ggacagatct
    33481 tcgcaaccgc agacccggca tacggttttg ccgtcatgct catgcggttt gatggcatca
    33541 atgaaaatgt tgttgggatg gttgttgtcg attttgcaaa gtctgcgtcc catgatgcgg
    33601 ttcttggcaa tctggcggtc cagaacgatt tcgatgacgg aatccagttt gatgttttct
    33661 ttctgcagag catcccagag cgcttcagcc tgagcgagat tgcgggggaa gccgtccaga
    33721 agccatcctt ttttgcaatc gtcttccttg agacggtcca gaatcatggg gatggtaata
    33781 tcatcaggga cgagttcgcc gcggtcaata aaagcctttg ctttttctcc cagcggcgtt
    33841 ttcttcgaaa tattctgccg gaaaatgacg ccggtttcaa tatggggcac gccgtacttc
    33901 ttctgcacga ttgcgccctg agttcctttg ccgctgccgt tggggccaaa aattaaaatg
    33961 ttcacgttgt gagactcctt tcatggtaaa tattaaagtg aaagtcttat aatgtattgt
    34021 aagcatttta gcaataataa aagaaagaac cggaaaaaac gccaaaaccg gatacggacg
    34081 actcaatggc cgccgcagga agaacaaccg gaacatgacg ttgatgtgca cgatgagcag
    34141 ccggattttc cgcccgcaaa aaccgacatg attttttccg catttttcgc tttgcatttg
    34201 ggacagatca tgcctttttc ttctttagga gagaaaatca ggctttcaaa gatatgtttg
    34261 cactttttgc atcgaaattc atatatgggc attttatgga atcctctttt tcgttaaaat
    34321 atgcagactt ctgacgaagt caagttcgat ccggtcatag aaacgaattt tgttcatctt
    34381 tttttattgt tcttttttgc ttgtctttgc ctgccgtcag ttctttgacg gcccggtgaa
    34441 tttcggcgtt tcgttccgaa cccgggggga aaaaattcag ggcttctctg aaatgaaaaa
    34501 gggcgctgtc ttttttgttg gcctgcttga aatgcaaacc gaaataataa tgcgattccc
    34561 ctttattttg gagtttgccg tagctcatgg ccgtaaagta gtttagattg gccgcctcat
    34621 tagacgccct gtcccggata ttcaaaaaac aatccagcgc ttcccggtat tttccggacg
    34681 agaaatacgt tttcccgagc gcaaaggtaa tttcatcatt gcgggggtct gatttttttg
    34741 cacgcaggag atagttctgc gcctgttcat atcggccggt tttgagatga agcaggccga
    34801 tgcttttcag aatctcgtga tcgtcaggcg attgaacgag ggctttctga agatgctcaa
    34861 gagcagagct gttggctccc aactgatctt cgatcatggc caggttgtag agcaggtcgg
    34921 tattttgcgg gtctttggct aaagacgtct ccagttgtct ttgccgtctt gacagttcgg
    34981 aggcgcttaa aggaacgctt gcctggaccc gacacaggtt gccgatgatg ctgtttttcc
    35041 ctggctgccg gtatcgagtc ataatcaaac catccatgta aatgatacgg tcatcggttc
    35101 cgggatgtgt ctgtagataa gatggcatgg tcttggagag aaattcgtac tgtttcatga
    35161 ttttcagaaa atcgaccatc gccgacggat tatatcctgc gtggacgaga taatctattc
    35221 ccaggcggtc ggcttcttct tcatgctcac gcatatagcg caacgtcatg ctgctggacc
    35281 cggccagaga aaatgcggta atcgcggccg atgcttctcc tcctccgccg aggaaaacac
    35341 cggccagcat ggcagccagc gccgccatat ttaatttctg agatttggaa ataatgctgg
    35401 cgacatgacg cgcattggcg tgaccgattt cgtgcgcgat gacaccggcc aactgggctt
    35461 cattctccgc aaccagcagc aatcctttat tgatgtaaat ataacctccg ggagtggcga
    35521 acgcattgat ggcggaactg tcgaggataa aaaaattaaa tttgaaaggt gtccgggtgg
    35581 tttgatccag aatcagattg ccgataccgg tgatataatc tgtgagcact ttattatgga
    35641 gaatcatctg atgctgttct agttgctcat agaattccct gcccagtttg ttttcatcct
    35701 caatggtaaa ttcagcacga caaatgtccg acgtcaacag cagcatcaat acaatcatgc
    35761 ccaaataaat atattgattt tgaatgaatt tgcacataag cattttccga tttcacattt
    35821 caagcaatgt tacattcttt ggattttaaa gacaagtttt catttaaaaa taacaaaaag
    35881 gattatacgt gatgaagaaa aaagctgtga tcaccataga tggtcctgcc ggagcgggca
    35941 aaagcacgat cagcaagatc ctggcaaagc gactgggtta tatttacctc gatacagggg
    36001 ctctttacag aggtctggcc tataaggcct taaaaatccg aatctccatt gacagcagcg
    36061 cagagctgtc cgaattatgt tcaaccacga aagtcgattt gaaaaatgtc aacggccgga
    36121 tgagggttta cgtggacggt gaggatgttg aaaacagaat ccggacggaa gaagtcggtc
    36181 tgattgcctc taaaatatcc acctttgtgg ttgttcgcga aaaactgctt gacctgcagc
    36241 gggaagccgg attgcgggga ggtcttgtcg ccgaaggaag agatatgggt tcggtggttt
    36301 tccaggatgc cgattataaa ttttatttgg atgcgacact tgccgaaaga acaaaaagac
    36361 ggcataagga actcttactc aaaggttcga cggcagaata tcagtcaatt caaaacgata
    36421 tgtcgatcag agacaaacag gatcaggaga gggccattgc gccgttgaag ccgcccgaag
    36481 ggtcgatcat cgtcgattcc gataatttgt cgattgagga ggttgccgaa aaaattatgt
    36541 ccatgatttc aaaaaagtag cctggtattt aaataaattt tacttgcatg atttatgttg
    36601 atatttaata tttggtatgt taaaccttaa aaattactgg gtttcataaa aaacaggggg
    36661 tattggttta atggttaaca ataacaactc aaacttatca aataaaaatg aagcaggtga
    36721 aaaatcctta ccgaagaaca atataacttc cggtaaagaa gaaatggatt ttaaagagct
    36781 ttacgaacaa agccttaatc aatttcagta cggtgatata gccaccggaa aagttgtgca
    36841 gataaaagac gatcggatca tggtcgatgt cggctggaaa acggaaggtt ttattccgat
    36901 cgacgaagtc agagacgcgc agggcaacgt aaccatttcc atcgacgatg aaatcgatgt
    36961 gtttattgac agaagagact cggaaggaaa tctggttttg tcccgtgaca aggcgtcaaa
    37021 agtaaaaata tgggatgaaa ttaaaaatgc ctgcgaaaat gataccgtcg tggaaggcgt
    37081 tgtcgtggaa aaggtcaagg gaggattgtc cgttgatatc ggcattatcg cttttcttcc
    37141 aggatcgcag gtggatatcc gtccggtaaa ggatctggat aaattcgtag gtcagacgct
    37201 ggattttaaa gtgctgaagt tcgacagaaa gcgcaacaat gtggttttgt cccgacgctc
    37261 cattgtggct tcggaaagag aagcggaaaa gcaggatatt ctgaaaaaca tccaggaagg
    37321 caacgttgtt gaagggatca tcaagaatat tacggattac ggtgttttca tcgatctggg
    37381 cggtgttgac ggactgctgc acgtgaccga tatttcttgg gggagaattg tcaagccgtc
    37441 ggaaatgttt cagaaaggcg ataaaattac gaccaaagtt ctttcctttg atgcggagaa
    37501 ggaaagggta tctctgggcc tgaagcagtt gacggcaaat ccctgggaaa aaataacgga
    37561 aacctatccg gtgaactcta ttgtaagagg caaggttgtc aatttgaccg attacggtgt
    37621 ttttgtggaa ctggcatccg gtgttgaagg tctggttcat atctcggaga tgtactggac
    37681 aagagaaata aaacatccgt ctaaagtgct caatatcgga gaagaaatcg acgtcaaggt
    37741 gctggaggtc aatgctcaga acaagagaat ttccctgagc ctgaaacaga cgacgccgaa
    37801 tccctgggaa aagctcaaag aaaaatatcc ggttggcaca atcgtaaaag gcattgtcag
    37861 aaatatcacg aatttcggcg tgtttgtcgg aattgaagaa aaaattgacg gtctgattca
    37921 cgtgtcggat atttcctgga aacaccgggt taatcacccc tccgaatatt tcaaaaaagg
    37981 ccaggaagtt gaggcagtcg tcttgaatat tgacgtgcag aatgaaaagt tctctctcgg
    38041 cgtcaagcag atcgagaaga atccatggga tgaactggcc cagaaatatg ctccgggcag
    38101 tgttgtgacc ggcaagataa ccaactttac cgatttcggc atttttatgg aaattgagga
    38161 aggcatcgaa ggattggtgc atatttctga aatcagtcag aagcgcgtca agacatcttc
    38221 ggaactttat tcggtaggcg atacggtttc cgctgtcgtg aagagtatcg atccgaaatc
    38281 gaaaaaaatc agattgagca tcaaagagct ggaagcaccg gcgccgaccc cgtcatccaa
    38341 tcagtatatt aataatacgg aaaatgtcgg ttccaatctc agccaagctc ttgctgacgt
    38401 aaaaattaat acgaaacaaa agggcgagta aaccatgcga aaacatcccg tgttgtttgg
    38461 aatgttgatc ctttttgtgt ttggtctgat cttctatctg tctttttaca acgccggttt
    38521 taacatggga aagacatccc ggttttcgtt aaaaaacaaa atcggcgttg tgcctgttga
    38581 aggcatgatt gccgattcgc tggaaattgt cgaacagttg gataaatttg gcaaagacgg
    38641 ctctattact gcggtggttc tcagggtgga ttcgccggga ggaggcgttg ccgcttctca
    38701 ggaaatatac gatgccatcc tcgagctcaa aaaaacaaag aaagttgtcg tttccatggg
    38761 atcgattgca gcgtccggcg ggttgcttat cgcgtgcgca gccgataaaa ttgtcgccaa
    38821 tcccggcacc ataaccggca gtatttcggc tatcatgcag tttgccaatt tcgaagagct
    38881 gctcaagaaa gtcggcgtaa aatccgccgt ggtgaaaagc ggcgaataca aggatatcgg
    38941 ctctccgtta cgggacatga cgcctgaaga aaaagtcatc gttcaggatc tggtggacga
    39001 tatttacaat cagttcgtgg acgtgattgt gaaagaaaga aaactgtccc gggaaaaagt
    39061 gatcgaaatc gccgatggac gggttttttc aggccgtaaa gcgaaggaat tgggattgat
    39121 tgattatttg ggcgacatgt cttccgccgc aaagctcgcg gataaattag cagggggcaa
    39181 cggcgaattc gatctggttt atccttcaaa gaaaagagcg tcgattttcg attatgttct
    39241 ggaaggtgcg gcgagtaaaa taagcgcgtc gctcaaggaa aaaatggaga tgaatagcgg
    39301 tttaagttac ttatattatc ccggaagata aaggaaagtt tatcatgaca aaaaacgatt
    39361 tgatcaaaaa attacaggaa gaactcaaaa cgtattccct caaggatgta acgtacgttg
    39421 tcaacatcat ttttgattcg atgattgacg cgataaaacg cggcgaacgc attgagttga
    39481 gggggttcgg cagtttcgag gtccgggaaa gaaaaccgcg catgggccgc aatcccaaaa
    39541 caggcactca ggtgaaactg acagaaagaa aagttccgtt tttcaaaaca ggaaaagaac
    39601 ttcgtatcat ggttgataat aaaaaatgac ttaccatagt ttgattgttg aaaagaaaaa
    39661 gaagtacgcc gtgattaagt tgaatcgccc tcatgaaatg aatgcgatca gcaaagagat
    39721 gcatcaggaa ttgtatgatg tatttgccga tctggaaacc gattccgatg tgcgggcagt
    39781 cgtgctgacc ggcggagagt atgtcttttc ggcaggtatg gatatcaagg aaatgaacaa
    39841 cctgcccgat aaagaaagcg ataaattcct gaagtccatg atgatgtact tgaaaaagat
    39901 atatgcgttt aaaaaaccgg tgattgctgc cgtgggcggg atcgctctgg gcgggggttt
    39961 taatctggtt accgtttgcg atcttgtcgt cgcatccgaa agcgccatct tctgtcatcc
    40021 ggaattgaaa ttcggattta atccgttttt ttacccgctg agtcagattg tcggcatcac
    40081 caaggcgaag gaaatcgtta tgctgggaga accgatcggc gccaatgaag ctatgaaact
    40141 gggactggtc aataaagtgg aaccgccgga aagattcatg gaagtggcgg agagtatcgc
    40201 ggatactttg tcacaacgtt ctccgagagc ttttgaggaa ttgaaaaatc tgtgctccat
    40261 cgttccccgc atggataaaa tggcggccat ggatattgaa tcgtcaatct gcgctctgct
    40321 ctttacacgc gatgagcgaa aagagcaaat gagagaagtg ctttcaaaag ataaatcagg
    40381 aagaaaatag agaaaacaga ttctggagcg gcgcgaacag aaatccgtga gtcataacgg
    40441 aacaatcata atgctcataa aagtacaccg caaaaaatca aggcccatag atcagacctg
    40501 aaaatcaatt cccctgaata gcatacaatt ttttggttta atttttcccg aacggatgtg
    40561 tcacaaaaat atctccgaag agaaactggc aggtatagaa tattgactgt gaaggcgaga
    40621 tagagttaca ggctgtccca gtcgttaccc ggtgcggcaa aatctttgag ttgatcacgt
    40681 ctctttttat gttggagttt cttcagagct ttggcttcaa tctgacgaat tcgctcgcgg
    40741 gtaacgccca tcatgtcgcc gacttcttcc agcgtaaacg gaccgtaatt gcaggcgacc
    40801 catgtgcaat ttagaaagga ttcgtttttc agacgccatt cgcaggttgt atcggtgcag
    40861 acttcactga tcagagtgtt ctttttcttg cacttataca tatttatcat ggagaaaact
    40921 ccttaatgat taagttttgc ttgaatataa taatttattt acggaaatca agagaaaaga
    40981 tgaaaaaaat tgcccggcct tccgacagaa tttgaaaatc attgaattga caaggactta
    41041 aaagccgatt ttttccttgg gccttctttc cggaacgtac tgatgttcga tttttccttc
    41101 attccattcg gctgaaacgt ataaattaca gaaacaactg ccgtattccc tgacatcgtc
    41161 cggccggtat tggcagggac agataatatc ggcgtccttt tcccggttgc cagccgccag
    41221 acggcaggga caacacatat agccataacg ttctttgttg actaaaagat cgccgaggat
    41281 gcttaaaacc caatctttat ctttattgaa aaagtaacct ttcggttcct ggaccttttt
    41341 catcatgtcg tagagttcag caggagtcat taaataccca aagcctcctt aatagcgttt
    41401 tccttgaaac cgatgatgac cttgtcacca atcagaatgg tcgggaaaga acggtcggga
    41461 ttgtactgca taacttcttc cagcattttc tgacgggcat cgccctgcag caaatcgaca
    41521 tccacgcaat caaaaggaag gctgttgtcg ttcaggaatt tctttgttgc cttgcagtga
    41581 ctgcatgtgc tcaacgtgta aattttaatt ttagcggcca tcttcttctc cttgaattta
    41641 gtaattctta tttaaccgga ttttataaaa atggcaattg gttatttttc agtacacgat
    41701 ttcttcataa ctctttctcc attttggcgg aagaagggtt tttgttttgt cgaaatggcc
    41761 tatggccaat aagcagggaa tccagtactg gtcggggata ttaaacgctt tgtgaacgcc
    41821 gtcaatatcg aatccgtcca tcgggtgaga atcaaggccg gcatctttgg cagcgagcat
    41881 caacgccata gcgaaaaaac cggtattctt gcaggcaaac gccagttgac gttccggcgt
    41941 agctccgtaa agagaattgg ttgccttgca gaaccagtcg tactgctcct gcttcatgat
    42001 gccctctttg accgattcga caaaatcttt ttcaaaaccg ggattgcctt tcttccatcc
    42061 ttcacgatcc gccagaacaa tcaggacgac gggggcttcg gtaattttcg gctgattcat
    42121 ggctactttt ctcaacattt cttttttttc tttatctctt agaacgataa ccctccatgg
    42181 ttggagattg aatccggaag gcgccaacgc cgcggtttct atgatctgtt taagctgcgc
    42241 atcggttacg tttttggtgg ggtcaaaaaa atttacagcc cgtctttgtt ttataacatc
    42301 tttgaattcc atagtaacac ctccaatctt gacgttgaca tttaattaat atccattatc
    42361 cgtcattccg gtgtaaaccg gaatctggga aattattgac tcgcttacgt tagctggata
    42421 ccggcctgca ccggtatgac attactaata ggttatcttc tcaaaaaaag atattactat
    42481 ttctcttcgt gtcccttagt taaataaaaa gttgccaggt cgaaaaaaac cgtcatcctt
    42541 cgttgaagtt cgaaggtcat cagcagctcc agatttttct gcagggcgtc ctgcgccaag
    42601 gcgaaggtcc agatgtgttt gcgaatgaga ctgagtgcgc tcaaggcgcc gctgatttta
    42661 aagcctgaaa tccttcgttc tttacccagt ttggtataga aattcctgat tttgtcaaca
    42721 tcgtgcaacc ctccgagcca gagggttaac tgagagagaa ttttgtcgca gatgttatac
    42781 agaaccgtcc gttccacctt gtgatactcg gtagtggaag gattgactat gacgtcttcc
    42841 agccattgtt caataatttt ttctttgttt ttgatgatga cattcaccag gcgtcgcgtc
    42901 agagaatttt ttgccacggc gggtggcagg gattttttgt
//
LOCUS       DAOOLB010000013        41197 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_705_length_41197_cov_43.018400, whole genome shotgun sequence.
ACCESSION   DAOOLB010000013 DAOOLB010000000
VERSION     DAOOLB010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 41197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41197
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_705_length_41197_cov_43.018400"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="PLF77_03120"
     CDS             complement(<1..1134)
                     /locus_tag="PLF77_03120"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPC07876.1"
                     /translation="MKRNKQVVPLIAVIILLSCLIVDGCGKKEETMAPPSPEVAVVTV
                     QSRQVVTTTELTGRTASNLIAEVRPQVGGIIQKRLFTEGADVRAGQPLFQIDPAIYQA
                     AVSNAGAALARSEAQLTTVQLRADRLRELLAEKAVSQQDYDDAAAALKQTQADIQYGK
                     ANLETARINLQYTTITAPISGRIGRSGITEGALVTAHQPLALATIQKIDPMYVDVTQS
                     TTDILKLRRKLQEGQLSRNSNQQQVRLILDDGSEYPFKGKLQFQDITVDPTTGSVILR
                     IVFPNPKGLLMPGMFVRAIVEEGVHKNAILVPQQAVSRDPKGNPLTLTVDKEGKVQQK
                     PLTLDRAIGSEWLVSSGLEPGDKVIVEGMLKVKPGIPVKAVPFD"
     gene            complement(1181..1795)
                     /locus_tag="PLF77_03125"
     CDS             complement(1181..1795)
                     /locus_tag="PLF77_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPC07877.1"
                     /translation="MISNRQNIINATDRLMQTHGLARLTTRAIAREAKVAEGLIYHHF
                     KDKAELIFEVIETRVRETKNYMQNLPLEVGKSTLTKNLENVLLSVYHAHYEITPIINT
                     VFADQKLRVRLQEIVKERNMGPRYAIEGLEVYLSAEQRLGRLSDAIDTGVLAKCLWMI
                     SIQSAMLDRWTENEVDEASVSAEIRKYIQTLMTGFAPRPKPKKR"
     gene            complement(2027..2743)
                     /locus_tag="PLF77_03130"
     CDS             complement(2027..2743)
                     /locus_tag="PLF77_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07878.1"
                     /translation="MENQNHYYIIWAIRLLLIISILANIVFITLHLLSDDLVTKKEII
                     EELSQRIELLFWSVVTLTLTFLPDYIEYKNIHLPHILEIIIVIFIFAGIFLSVRFELY
                     YNVFWWDDVLHTLSGVIIGFLGFIIIYKINSKYSMDISPLLVAVFAFTFAMTMGVIWE
                     IFEFAMDVFFGTTMQSWDLPHDTTLIGRAFQGSGLRDTMSDLIVNSLGALLTSVICYF
                     LYKREKKKALALMHEIFPDA"
     gene            complement(2835..3188)
                     /locus_tag="PLF77_03135"
     CDS             complement(2835..3188)
                     /locus_tag="PLF77_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07879.1"
                     /translation="MMKKFFLFLAILFLFSCAQIVRNPVKYDQQVFINHYDKMFSAAM
                     NQGTRMSYKIDYQNREYGLLKMSRKVGVSTYRITVKFDDDNFTIKGDIDTDIFNPFIG
                     EDAKIIEEAIKNAVK"
     gene            complement(3227..3763)
                     /locus_tag="PLF77_03140"
     CDS             complement(3227..3763)
                     /locus_tag="PLF77_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07880.1"
                     /translation="MTGTKQEFHFPGEETMKNTNFLIIILLTVFLLSAADSWAKNWTL
                     LGTDPNGTEYYKPSDVKKVTKNIVHVRTKIIYNERGKFRNFSRLTKTEKNLRNPYILN
                     FEMKWIEFNCTTRQYRILSGGIYDKRGKLISDLPPSGKKWSRIPAGSPAEKLMDILCG
                     NTDVTKNNGSKNKKVDPE"
     gene            complement(3975..4502)
                     /locus_tag="PLF77_03145"
     CDS             complement(3975..4502)
                     /locus_tag="PLF77_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPC07881.1"
                     /translation="MNELVIGAVTGILFGFVMQKAQVIRYDRQLGALRFKDMTIVKFM
                     LTTILVAMVGIYLLFDLGLVKLSIKPLILGGNVLGGLIFGLGWGVVGYCPGTAMGALG
                     EGRYDAVFGLLGMILGAGIYAELYPTMKTTVLTWGDFGKITLPAVLGINHWFVIIIVA
                     AAFLGLLGWFEKKGL"
     gene            complement(4495..5055)
                     /locus_tag="PLF77_03150"
     CDS             complement(4495..5055)
                     /locus_tag="PLF77_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(4621..4623),aa:Sec)
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPC07882.1"
                     /translation="MRFDSTLKEVKMMNVFREKTWSPYAAGALAGLLLILSVFITGKY
                     FGASTTFVRVAGYVEQTVTPDKIAGMEYFIKNKAKIDWQFMFVVGIFFGALAAAWLSG
                     EKRMVAVPKMWEERFGPSRAKRWTAAFLGGIVLMFGARLADGUPSGHGLSGLAQIAFS
                     GYVSLICFFLGGILIARIVYGGNHHE"
     gene            complement(5145..6239)
                     /gene="rlmN"
                     /locus_tag="PLF77_03155"
     CDS             complement(5145..6239)
                     /gene="rlmN"
                     /locus_tag="PLF77_03155"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPC07883.1"
                     /translation="MTGRAEHSLPNLLDFTQEELQDFISVLGKEKFRAQQIMKCLYQS
                     GYTSFDEMTTLSRDFRTALSQIARISRPHIVKVQESKDKTKKTLLRLEDGRFIESVLI
                     PGKNNWTICVSTQAGCAMDCKFCLTARQGFQRNLNPSEITGQILTLKHELPGCPDIKN
                     IVMMGMGEPLANYDNVVTAIKNITGDFGLGFSNRKVTVSTCGLAPQIIRLGKDICINL
                     AVSLNAHDNDTRSGLMPVNKKYPLEILLDACRQYPMPGRRLLTFEYILMDGINSSVQD
                     AKKLAALLKGQRCKLNLIVFNEYPGSPFRSPSPKTVEAFQKILIDAHYTTMLRASKGS
                     DILAACGQLSGTYNDQVSHCSFQKGSKFLT"
     gene            complement(6236..>6526)
                     /locus_tag="PLF77_03160"
     CDS             complement(6236..>6526)
                     /locus_tag="PLF77_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HPC07884.1"
                     /translation="VQKHYYKHLKGEETSSNSMALIFAWSGGLRQRGHMDGTPDVVTF
                     ADTLEEAAIATVERGIMTGDLLALAEKKASNKKVNTEGFIDAIAATLQEKLP"
     assembly_gap    6527..6626
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6627..7611)
                     /locus_tag="PLF77_03165"
     CDS             complement(<6627..7611)
                     /locus_tag="PLF77_03165"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HPC07885.1"
                     /translation="MAAKIKVKTTVVEMDGDEMTRVMWQMVKDKLLFPYLDMDIDYYD
                     LHVKHRDDTDDKVTVDAAKAIMKYGVGIKCATITPNEDRVKEYRLKKAWKSPNGTIRA
                     MLDGTVFRKPILVNNIKPNVSSWKKPIIIGRHAYGDVYSNTEFDVPSAGKLELVFTPQ
                     DGGKAVNVPVFDFPGKGIAQVNYNLEQSILSFARACFTYALSEKIDLWFSTKDTISKI
                     YDAGFREIFQQEFEKNWKNKFDQAGIEYFFTLIDDAVARVMKSEGGMLWALKNYDGDV
                     MSDMVASACGSLAMMTSVLVSPHGWFEYEAAHGTVQKHYYKHLKGEETSSNS"
     gene            complement(7754..9685)
                     /locus_tag="PLF77_03170"
     CDS             complement(7754..9685)
                     /locus_tag="PLF77_03170"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HPC07886.1"
                     /translation="MAQNLVEKILLKHLVSGTLIPGEEIGIRIDQTLTQDATGTMAYL
                     QFESMNMPKVKTELSVSYVDHNTIQIGFENADDQLYLQTVAQKYGIVFSRAGNGICHQ
                     IHLERFGKPGKTLIGSDSHTPTCGALSMIAIGAGGLDVALAMAGKPFYLVCPKVIKIK
                     LTGKLKPWISAKDVILKVLEIFSTKGNVNTVFEYGGKGVESLTIPERATIANMGAECG
                     VTTSVFPSDKMTRLFLRSQHREKDFVELKPDKDAKYVKVVNIDLSKIEPLTAKPHSPD
                     NISTVKKMQKIDVHQVCLGSCTNSSYKDLVTVAKILKGKTVHPNVSFILAPGSRQVLK
                     ELSKNGYLTDLISSGARLMENACGFCIGNSQSPPSAAVSLRTSNRNFQGRSGTMDANV
                     YLVSPETAAAAVITGKFTDPRDLKMRYPKVTLPKKFSIDDTMIIRPDKKKKEIEVIRG
                     PNIGILPRSPELSDTIHGPVTIKVGDKITTDHIIPAGARMKYRSNIPKYSEFVFENVD
                     ATFAKRAMSIRQDGKQNIIVAGHSYGQGSSREHAALCPMYLGVRVVIAKSFERIHTAN
                     LINFGIIPLTFCSESDYDIIEMEDLLEITETKETIAGDGKFLMRNKTKGTAFYVTCDL
                     SERQKQIILAGGALNINNA"
     gene            complement(9889..>10598)
                     /locus_tag="PLF77_03175"
     CDS             complement(9889..>10598)
                     /locus_tag="PLF77_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HPC07887.1"
                     /translation="YVVKKGDTLSKIAQKNRIPLKKLMALNNIHSKKIRAGQKIVLRA
                     SDPPSRVKTVVAEDRDEDIIDDTENAAVNEDPAVEENYDGEEGFFSNIFNFGYQDDFL
                     GKWKSTDEMQRLIKKATGFLGAPYRLGGNSKRGIDCSALVQKVYGSFNVKLPRVARDQ
                     SKVGVEVDIDELVEGDLVFFHTNRSFGHVGIYIGNNEFIHASSRNRRVRIDSLDHPFY
                     QERFQRAVRIKELPVGG"
     assembly_gap    10599..10698
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10699..11021)
                     /locus_tag="PLF77_03180"
     CDS             complement(<10699..11021)
                     /locus_tag="PLF77_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPC07888.1"
                     /translation="MKRQMTVFSGLFVLFLLFGLSSNAFAIHYKVKNGDTLYSISKKF
                     NVSVIDIKKANNLKKTTIKANQVLKIATSPKGTSKSTVSKNTISSYYVVKKGDTLSKI
                     AQKNR"
     gene            11417..12322
                     /gene="mtgA"
                     /locus_tag="PLF77_03185"
     CDS             11417..12322
                     /gene="mtgA"
                     /locus_tag="PLF77_03185"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HPC07889.1"
                     /translation="MRFFGKIISIILVLFLAYFIIDVGRYFVFPWVGSLAKSSPEKTA
                     FMKHREKMWEKEGVNKKITKIWVPLSRISPYVMKAVIIAEDDKFWSHEGFDFEAMQKA
                     LEKDIKKKKFKAGGSTISQQLAKNLYLSPSKNPIRKVKEAILTWRMERNLSKRRIMEL
                     YLNLAEWGDGVFGIEAAARKHFGKSAAALGPREAAVLAAILPNPIRYSPHGGSKYVAY
                     RSERIYQIMVRRGIVIPEYEEVISETTDESEEETEEQIEESVPEVTEQESAPVQQTEE
                     QKEESVQEEQKATPVLPETEKKSIP"
     gene            complement(12335..13588)
                     /locus_tag="PLF77_03190"
     CDS             complement(12335..13588)
                     /locus_tag="PLF77_03190"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPC07890.1"
                     /translation="MKDVVIVSACRTAIGTFGGTLKNVIGANIAAVVMKEAIKRAGIE
                     PSIIDDVRFGCCLESCNTLNVARVGALLAGIPESVTAVTMNRVCISGMEAVLSGAAMI
                     KAGMADIILAGGVEHMSGTPYVSFDARWGCRLQDTTFIDSMIRALHCGSYVMPLSDDG
                     PVKSGQPFESLKGKPYIMGHTTELIAQLLNISREEMDEVALRSHNNVERATKDGSFKE
                     EIVPIEIPQKKGKPPIIFDKDEHFRPGLTMDQLKALPPAFIPKVGKVTAGNSSGLNDG
                     ASAMIIMSAEKAKELGLKPIARIRSAGRGGCHPSVMGLSPVPAVKNLLKNDPKLTLDS
                     FELCEVNEAFAGQYLGCEKELGLKRDITNINGSGIGLGHPVGSTGSRIMVTLIHAMRK
                     HGKTLGLATLCGGGGVSMATAIEIL"
     gene            14091..>14314
                     /locus_tag="PLF77_03195"
     CDS             14091..>14314
                     /locus_tag="PLF77_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07891.1"
                     /translation="MNKKIFCLAVALCVSAFFLISCSGGKNAADIAIKAAEEAVNATK
                     AEAAKVVPDEVKSLEDSLAAVKEKFVKGEY"
     assembly_gap    14315..14414
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14415..14848
                     /locus_tag="PLF77_03200"
     CDS             <14415..14848
                     /locus_tag="PLF77_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07892.1"
                     /translation="LEDSLAAVKEKFVKGEYKAALEEATALSARAKEVLDAAKAKKEE
                     LTGKWTEMSQGLPKMFEDIQGKVDGLSKLKKLPASLTKEKFEEAKATLASAKDEWAKA
                     QTSFAGGDFSTAVSMATVLKDKALKIMETLGMSAPEAAPAK"
     gene            complement(15026..15643)
                     /locus_tag="PLF77_03205"
     CDS             complement(15026..15643)
                     /locus_tag="PLF77_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HPC07893.1"
                     /translation="MEIILGIAVGIGLSAACGFRIFVPLLIMNLAALSGQIQLPAEFA
                     WIGGTYATIAFTCATLIEVLAYYIPGVDHILDIIATPAAVIAGTITTASMSMDMSPFL
                     KWTLALIAGGGIAGLVQGSTVALRTKSSMTTAGTGNVIVATLELIGAVVIAVMAILLP
                     VVCLVIIVLFIAFAFKKVGVFIFRRFRKPIKQTDKNAFSRRLPRG"
     gene            complement(15677..17014)
                     /locus_tag="PLF77_03210"
     CDS             complement(15677..17014)
                     /locus_tag="PLF77_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPC07894.1"
                     /translation="MKDIMKKIRQLANFIAHDIWRIRRSQLSAGKSFLIKQARVLILS
                     LKGFHEDKCQLRASALTFYALISIVPVAAMAFGIAKGFGLKEALETQLRSRLAGQEEI
                     AANIIAFSHSLLEKTQGGLIAGVGLVVLMWAVFKLLGQIESSFNDIWKIKEKRSIGRM
                     FGDYLALIIICPVLLILSSSTTVFITTQVSVILEKFTVLGCFSSFIFFLLKLLPYMLM
                     WFLFTFLYIFIPNARVRFSSGLVAGIIAGTIFQIVQWAYITFQIGVVHYNTIYGSFAA
                     LPLFLVWLQLSWTIVLYGAEVSVAYQNADTYEFEPDFLQASHRMRILLSLLVTSRVIK
                     NFIRGEKPMTALDISSQLGIPIRFVNEILFDLIKSRIFSVVEIEGEDRRGHQPAIDVS
                     GLTIQDVMDALETRGMNTLPLSDQDGLKSLADSMDAFFRKIETLPENRCLKDI"
     gene            17242..17826
                     /locus_tag="PLF77_03215"
     CDS             17242..17826
                     /locus_tag="PLF77_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07895.1"
                     /translation="MHKIFFRKMNEKWKTFFFLALIFAISSCYYYPQEQMVTRPSPDE
                     NIRAITQIYFYPNRNQSADLQSRDHYACYTWAMEQTGFDPSVSSIVPEQRVRVVPMPP
                     PGQDTIAMSIAGAVLGALIAGPRHAGGGALIGAAGGAMAGMVSDASRVEAARQMEEAY
                     NNRNQGRDFRREKKGLQFRRAMSACMEGRGYTVK"
     gene            17851..18597
                     /locus_tag="PLF77_03220"
     CDS             17851..18597
                     /locus_tag="PLF77_03220"
                     /inference="COORDINATES: protein motif:HMM:NF040452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6515 family protein"
                     /protein_id="HPC07896.1"
                     /translation="MISVKFNFKKCFVVVLSLFMLTFVSELVFTPASQAQPSRKSSVR
                     TVKKGSTVHHRKQFFDARHSHNRAYPVRGHYFRSLPRDHRVIRHGSSRYYSTSHGVWY
                     RPYRGLYEVIAPPIGIFVPFLPLFYTTVRVQGTPYYYANGTYYTQTPGGYVVVHPPQG
                     EVMENPPVTDDEDEGFMDDKLFIYPSKGQSEKQRDLDRYECHQWALEQADYDPTQISS
                     GRSADEVMQGRVEYRRAIAACLESRGYAVK"
     gene            18679..19164
                     /locus_tag="PLF77_03225"
     CDS             18679..19164
                     /locus_tag="PLF77_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07897.1"
                     /translation="MERQKILQQMIDLNKIFFDSWFSMMAMFQHQTEEILKPFVDCMP
                     GMDGRMKKFIRHWSEECKKNRETFKRDIDAGYAKAEAFFDYHAILKFQEQNEQLLNDF
                     LSQTTWMPDDFKTAAEKMADVYKDGCDKFRRYVEKNINGVSDFSSSSGKPGRKAGSRK
                     K"
     gene            complement(19216..20652)
                     /locus_tag="PLF77_03230"
     CDS             complement(19216..20652)
                     /locus_tag="PLF77_03230"
                     /inference="COORDINATES: protein motif:HMM:NF028290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HPC07898.1"
                     /translation="MMKVVHRALILFLTVVFPAVVFAGGFGLDSSTADIGTLLPLWTT
                     LPFTGILLSIALIPLLLPRFWHRHFPKVSAFWAIVFAVPFIFFFREKALHEIAHIILI
                     DYVPFILLLWALFTVSGGIYVRGTLKGTPAVNTTVLLIGTLLASVIGTTGASMLLIRP
                     ILRSNAWRIHKVHTIVFFIFLVSNIGGSLTPLGDPPLFLGFLHGVPFFWTMHIFPEMA
                     VASVTLLLIYFALDSHYYKRENKTAIPEEEFEPLKIEGGRNFIFLAGIIGAVLFSGSV
                     RLGDVNILGIHQTTENLIKDAVLILMGLASLGFTAKEVRKNNDFGWAPMLEVAYLFAG
                     IFITIIPALAILKAGEKGALHWLIKAIDSPAQYFWAVGILSSFLDNAPTYLTFFNSML
                     GKFYPGMPEPAAVALLIVEKIPYLAAISVGAVFMGANTYIGNAPNFMVKSIAEEAGVK
                     MPSFFGYMFKYSIPVLVVLFIVMTFVFF"
     gene            complement(20649..21710)
                     /locus_tag="PLF77_03235"
     CDS             complement(20649..21710)
                     /locus_tag="PLF77_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HPC07899.1"
                     /translation="MNLMQESYLGNTLQAWLIALSILIVVFIILKLIQAVAVRKLSRL
                     AVSTSNQIDDLLVGLLKRTKGFILIIAAAYLASLTITLKPSVGQLWQKVVILALILQA
                     GFWASSAIAFAIGNIIKKRSEEDFSSKTTITFLGFVARFVLWIVILLLILDNLGVNIT
                     GLVAGLGIGGVAVALAVQNILGDLLASLSIVLDKPFVIGDFITVDSLSGTIEHIGLKT
                     TRVRSLSGEQLIFSNNDLLKSRIRNYRRMSERRIIFNFGVVYQTPLEKLKTIKMIVRD
                     IIDKTETARFDRVHFKEYGDSSLNFEAAYFVQSSDYSTYMDVQESINLEIFRRFRDEG
                     IQFAYPTRTVFIQDEKQQK"
     gene            complement(21786..22196)
                     /locus_tag="PLF77_03240"
     CDS             complement(21786..22196)
                     /locus_tag="PLF77_03240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPC07900.1"
                     /translation="MLNFKNILYPIDLNSEKISSLETALEFARLLNGALHIFYVNDIL
                     AGYRHPTDHEDAVALRVKETVDASLLENINIIYAVSKGETTEEIVKYAQDNQIDLIII
                     GHKHRGRLYASLFDSTDIDTIDKVFLPVLVIPEK"
     gene            complement(22517..23284)
                     /locus_tag="PLF77_03245"
     CDS             complement(22517..23284)
                     /locus_tag="PLF77_03245"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPC07901.1"
                     /translation="MGEAVTKEIRDGVAILAIDKPKMNQLSGEVFEAMRKHLDAVEAD
                     KAIRCVIITGAGEKAFSAGADLASGFGDFSAVDFLKRGQDIWNKVEYFPKPVIAAING
                     HALGGGCELAMACHIRFMKKGARIGLTETNLGIMPGYGGSLRLPRLVGKSKALEMIYL
                     GKQCEADEALSIGLVDHITEGNVVDAALELANKIVKRAPLAVAAVTKLCNISPSISAE
                     IHLKIEREELGKLFNSKDAQEGIGAFFEKREANFKGE"
     gene            23851..24618
                     /locus_tag="PLF77_03250"
     CDS             23851..24618
                     /locus_tag="PLF77_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPC07902.1"
                     /translation="MAYDNIIVETKDFVTVVTLNRPPMNSINLGIREELDQIITDVQT
                     SKETRVVVVTGSGEKAFCAGMDVADVANIDKGPNGIDVCNKVERSLKPVIAVINGHAL
                     GGGCELAMACHFRFMTDNPKSQIGCPELNLALTPGWGGIQRMPRIIGKSKALDFILFS
                     KKVSGPDALAIGLVDKVFPAADLMKEAMHFAQALSKRAPIAVNAVLNGMNIGLEKGIV
                     AGLEADKQWIEKLKTSKDATEGVVAFIEKREPNFVGE"
     gene            complement(24863..25771)
                     /locus_tag="PLF77_03255"
     CDS             complement(24863..25771)
                     /locus_tag="PLF77_03255"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC07903.1"
                     /translation="MSNISFEGKVVIVTGAGSGLGREYALEFARRGASVVVNDLGVAR
                     DGSGSTKNAADAVVTEIKNSGGKAVANYDSVATPEGGINIVKTAVDTFGTVDVLVNNA
                     GFLRDKSMMKMTADEWDAIMSVHLRGAFCVTQPALAVMREHNYGRIMVTSSTSGIFGN
                     FGQINYGSAKMGLVGFMNVLKLEASKFNVHVNAIAPTAYSRMTENLIPKELAPKLQAK
                     FNVPMVVYLCSEECQENGSIFTMGAGWYAKTAIVCATGACLGDGSRDIPAEEVRDNFA
                     KISDLTGAKPLYDSMGIFELMGPLLQ"
     gene            complement(25813..26565)
                     /locus_tag="PLF77_03260"
     CDS             complement(25813..26565)
                     /locus_tag="PLF77_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPC07904.1"
                     /translation="MFAWLTSPEAWIALATLTALEIVLGIDNIIFISILVSRLPKNKQ
                     NFARNTGLMLAMLTRLALLFSIFWVIKLTAPWFTVFGQEISGRDMILIGGGLFLLAKA
                     THEIHTSLEGAQEETPRLISSSVAAVLIQIALLDIVFSLDSVITAVGLAKDVSIMAIA
                     IICAVGVMLFAAKPISKFVDAHPTIKILALSFLLLIGVTLVAEGFDVHVPKGYIYFAM
                     AFSVGVEMLNLRMRNKQMKPVKLHKTVYNPEA"
     gene            complement(26599..27213)
                     /gene="cobO"
                     /locus_tag="PLF77_03265"
     CDS             complement(26599..27213)
                     /gene="cobO"
                     /locus_tag="PLF77_03265"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPC07905.1"
                     /translation="MTMVKTEDPAVYNVRSKKSSGLKTSKENAVPKQKGLVIVITGNG
                     KGKTTSAFGQALRAIGQGYKVFVLQFMKGRKYGEFIAAEKYLSNITIKMSGLDSFVMR
                     DHPAAIDIDMARKGLDIARKAIMSGKYDMVILDEINVALDFKLVDLKDVMKLIKNKPA
                     HVDLILTGRYAPKEIIKLADTVSDVQEIKHHYNAGIKDRAGIEY"
     gene            complement(27210..28184)
                     /gene="meaB"
                     /locus_tag="PLF77_03270"
     CDS             complement(27210..28184)
                     /gene="meaB"
                     /locus_tag="PLF77_03270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPC07906.1"
                     /translation="MDATIKKICAGDVRAASRLIRDIEDKRPDAKVKIKKLYAHTGKS
                     FIIGITGAPGAGKSTLTDALISEFRKLNKTVGVLAVDPTSPFTGGAILGDRIRMQRHA
                     ADDGVFVRSLATRGALGGLSQAAGNAIHVIEAMGKDIIIVETVGIGQQEIDIINHAHS
                     VVVVLVPGMGDEIQTMKAGLMEIADVFVINKANRPGTGQLQTELSSMLNLSPKNADGW
                     KPPIVLVGDAFERATFDENTAELARKIEEHHCFLEQSGQLTERMHRKVMAEIKDALGA
                     CLMEPLMQSLISSGEMGKLSEKIKNRKSDPYTVAEDITEKFFQKQVKK"
     gene            complement(28203..28604)
                     /locus_tag="PLF77_03275"
     CDS             complement(28203..28604)
                     /locus_tag="PLF77_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HPC07907.1"
                     /translation="MSERKIRVMVAKPGLDGHDRGARILARCFRDAGFEVVYTGCHQT
                     PEQIAASAIQEDVDLVGLSCLSGAHRALFPRVVELLREKGAGDIAVIGGGIIPEQDVP
                     MLYESGIKAIFTPGTPLDSIVEWIKKNIKTH"
     gene            complement(28752..30434)
                     /locus_tag="PLF77_03280"
     CDS             complement(28752..30434)
                     /locus_tag="PLF77_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPC07908.1"
                     /translation="MYFSEEMQEASLKLTQKWEKEVQKTLKNNPEQKKRFSTVSDTEI
                     KRLYTPEDIKDINYTEDIGVPGEFPYLRGNQVTGYRGRYWTFRMFSGMGSAQDTNRRW
                     HMLLREGQTGLSTAFDFPTLMGYDSDSPKALGEVGKCGVAIDTLEDFLTLMEGIPMDK
                     VTTSMTINPPATVLWAMYCAAADIKGIPLSKIGGTIQNDMLKEFIAQKTFMCPPEPSV
                     KLISDTVEFGTKYVPRWNTISISGYHIREAGATAVQELAFTIRDGIEYVDDVIRRKKM
                     NVDDFASRLSFFFNSHIDFFEEICKMRAARRMWAKIMRDRFHAKDPRSLWLRFHTQTA
                     GCSLTAQQPYNNVIRTTVEALAAVLGGTQSLHTNSLDEVLCLPSEHAVEIALRTQQIL
                     AEETGVANTIDPLAGSYFIESLTNEMEEKAWDYIHKIDAMGGMITAIEKGFPQMEISD
                     AAYRFQKQLDAGEKSMVGVNKYATDVTHPLPLVDIDEKVGEEQIKRLKSVRRKRDSKA
                     LKKSLEDVRNACKKGNNVMPCCIDAVKNLATLQEICDVYRDVYGEYRDPGLY"
     gene            complement(30644..30844)
                     /locus_tag="PLF77_03285"
     CDS             complement(30644..30844)
                     /locus_tag="PLF77_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPC07909.1"
                     /translation="MPEGKVKWFNANKGFGFIQQDEGGDVFVHFSAIQVEGFKTLNEG
                     QRVSFDIQQGKKGLEAENVKPI"
     gene            31245..31817
                     /locus_tag="PLF77_03290"
     CDS             31245..31817
                     /locus_tag="PLF77_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPC07910.1"
                     /translation="MKIKASVLIVLLAALWMTVSAGAAEVKPPQVGQGFPDVELSKPV
                     IAGELKYLGLSGSGSFRINQIKADVVIVEIFSMYCPHCQAEAPAVNELYQLIEENAAL
                     KGKIKIIGIGTGNSIYETDIFKKKYSVKFPLVPDGDFKIHKIMGEVRTPYFIVVKLKG
                     KKADVIYSKAGALGDNKTFLKEIIKSAGLK"
     gene            31827..32414
                     /locus_tag="PLF77_03295"
     CDS             31827..32414
                     /locus_tag="PLF77_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPC07911.1"
                     /translation="MKKKIILAALLICFLAPGAFALSDVFKNNVYETGELKPQDSVLK
                     VKVGQPAPDFTLKAVSGKTISLKDYRGKKNVVISFVPAAWTPVCSDQWPGYNIVKDIF
                     DVNDAVLLGISVDNIPTLYSWTNQMGHLWFDVLSDFWPHGAVADQFGVLRSDGISERA
                     ILIIDKKSIIRYIDVHNINKRPPLESIVRELEKLN"
     gene            32582..33217
                     /locus_tag="PLF77_03300"
     CDS             32582..33217
                     /locus_tag="PLF77_03300"
                     /inference="COORDINATES: protein motif:HMM:NF019959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC07912.1"
                     /translation="MEEKKRNRKRKEKENRRSTILKAARKLFLERGFKAVTVDSIAEK
                     AEVSKGSIYLCFESKEEIYTQILIADNISLNERIQNFSLIEASASQLLLEFARIYVDF
                     FLSDKELFRILMTFMMQTGQMHLTQQQSDELIRSTNENIKVISEIIQKGVDSGEFRPL
                     DNILQMQNAIWGMLNGVISLFLFTGNPIKRMERIHSTVQESMNVFIRGLKA"
     gene            complement(33292..33633)
                     /locus_tag="PLF77_03305"
     CDS             complement(33292..33633)
                     /locus_tag="PLF77_03305"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HPC07913.1"
                     /translation="MEIKYKIWIEENGKVIFGKGRDEILKSIEQQHSLNAAAKELGMS
                     YRAAWGRLKASEERMEKKLVETSEKEKALQLTAQARTIIERFEKLEKDVENILKEAEQ
                     EFKKLFQTMGK"
     gene            33857..34273
                     /gene="nikR"
                     /locus_tag="PLF77_03310"
     CDS             33857..34273
                     /gene="nikR"
                     /locus_tag="PLF77_03310"
                     /inference="COORDINATES: protein motif:HMM:NF003381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HPC07914.1"
                     /translation="MPGLVRFGVSLEEDLLVKFDRHIKQKKYTNRSEAIRDMIREELV
                     RKEWTENKEVTGAITLVYDHHTRELVTKVLDVQHDYHTCILSTQHIHLDHHNCFEIVV
                     TRGKSKEIEELFQKLKSIKSVKHAGFMMATKGKDLP"
     gene            complement(34288..35022)
                     /locus_tag="PLF77_03315"
     CDS             complement(34288..35022)
                     /locus_tag="PLF77_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC07915.1"
                     /translation="MSHHIVEFKNVSFRYPDGTQALQDISFRITHGESVGIIGANGAG
                     KSTLLMHLNGYLMPTSGTVNIGDVYLTKKTQPEIRRKVGIIFQNPDDQLFMPTVYEDV
                     AFGPLNLGLDDTAVRERVEFALHLVNSLDLKNKPPHHLSCGQKSAVAIASVMAMEPDI
                     LAMDEPAANLDPRSRRLLINLLKTFEHSKIIASHDLDLILDVCHRCIVIKEGRVAADG
                     PVSEILTHQELLEENNLELPLSMQKR"
     gene            complement(35019..35828)
                     /gene="cbiQ"
                     /locus_tag="PLF77_03320"
     CDS             complement(35019..35828)
                     /gene="cbiQ"
                     /locus_tag="PLF77_03320"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HPC07916.1"
                     /translation="MSFDIQYYNIGFLDQLSYKSTFVHRLDPRVKLCVTLLFLLTVIS
                     FSKYEIFALLPFFLFPVLLMTLGEIPFMFIFKKVLLVSPFAVFIGIFNPLLDSGQISI
                     VNGYTISAGWLSFISILLKFTLTVSTALLLVATTSFPGVCHALRQLGLPSVFVSQLLF
                     LYRYIFVLMEETMRIVRARNMRSFGDRGKGIKIFVRIAGTLFLRTVQRAERVYNAMLS
                     RGFQGDMPALKHFQFKTGDMIFAATVAAFLLIFRFCPATEILGRFAQGVFS"
     gene            complement(35931..36965)
                     /locus_tag="PLF77_03325"
     CDS             complement(35931..36965)
                     /locus_tag="PLF77_03325"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HPC07917.1"
                     /translation="MHMADALLSPAVGATMWAGSVAAIGYSSKKLKENIDNKTIPLMG
                     VMGAFVFAAQMINFTIPVTGSSGHIGGGMLLAVLLGPYAAFLAMASILTVQALFFADG
                     GLLALGCNIWNMGIYPCFLVYPLIYKPVAGDGKSARRVSVAAILGAIVALQLGAFSVV
                     LETLFSGISELPFGTFVLLMQPIHLAIGLVEGIITAGIILYVQQARPEILHAHAAARP
                     LDAGLSIKNILAAFVILAVITGGALSWFASTHPDGLEWSIEKVTGKGELEEATQGIAP
                     VLKDIQEKTAFLPDYGFKPPADEQNTEESPSVWPGIEPGTSVAGVVGSAIVLMFIIFI
                     GFGIRLFKKQ"
     gene            complement(37010..37792)
                     /locus_tag="PLF77_03330"
     CDS             complement(37010..37792)
                     /locus_tag="PLF77_03330"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPC07918.1"
                     /translation="MIRKHFINGLVWAVLFFIAGPSWAAHPLITDDTGTQGKGNFQLE
                     LTGQYDQDKENVGGVSIKATGVETAATLSYGMIDNIDLVLSLPYLWGREKENGSLVYK
                     EDGIGDASLEAKIKVFEKEGFSLALKPGISFPTGDDDKGLGTGKFGGQIFFIASQEVE
                     PFAFHVNLGYIRNENDADERENIWHASVAAAWEIVKDLSLVANIGVERNPDGEADDDP
                     AFLIGGIIYSIAENLDIDLGVKYGLTDSEADISALAGLAFRF"
     gene            38005..41073
                     /gene="fdnG"
                     /locus_tag="PLF77_03335"
     CDS             38005..41073
                     /gene="fdnG"
                     /locus_tag="PLF77_03335"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:38590..38592,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HPC07919.1"
                     /translation="MHISRRGFLTLSSAVGGGIALSSLGLNLKPTRAYADELDKMNRV
                     KAAKQTTTVCCYCSVGCGLVCSTDKATGKIINIEGDAEHPINEGSLCAKGAGFFDLTE
                     ANKHRLKKVLYRKPYGTEWEEKDWNWALPRIARLIKDTRDKGFVTKNAKGELVNRCET
                     IGHYGSSNVDNEECWLMSVKCRALGLVYIDHQARVUHSPSVAALGESFGRGSMTNHYC
                     DLKNADVILIMGSNAAEHHPIAFKWIMRAKEKGATVIHVDPRYTRTSARCDFHVPLRS
                     GTDIAFLGGMIKYILENNKYFKDYVVNYTNASFLVNKDFSFNDGLFSGYDSQKRKYNK
                     DTWTLDKDANGIPKRDMTLSDPRCVFQMLKKHYSRYTLDKVSSITGVSKDNLLKVYKA
                     YSATGAPDKAGTECYALGWTHHTVGVQNIRSMAIIQLLLGNMGIAGGGINALRGEPNV
                     QGSTDHCILYANLPGYLKMPAASLDTLEHYLKKCTPESKDPQSANYYSNYPKFFVSFL
                     KSLWDDKVTKENDFGYDWLPKIDDGKHYSTMHMFDAMYEGKIKGYFCVGADTAVSTPN
                     SNKVRKAMENLDWLVGENIFNNETYEFWRGPGVDPAKIKTECFLLPASASMEKEGSQS
                     NSGRWVQWKYKAAEAPGDAIPVGEIEIKIMAEVKKLYQKEGGPNASAITNLKWDYLDS
                     KGHMDVLKVSHQVNGLFTKDTVIEDKAKGTSTLFKKGQCVPTFGNLKDDGSTACGNWI
                     MAGSFDEKGFNKMTKRGKDDPTGLGLFPNWSFAWPVNRRIIYNRASCDIYGKPYNPKR
                     NILEWVGDKWVGDVPDGPWPPQADKAKGKYPFIMKQDGVASLFGPAMAEGPFPEHYEP
                     LESPLAKNPMSGQLNNPAIEIFKGEMDKVASASEKFPYVCTTYSCTEHWCTGALTRWQ
                     AWLLEMQPELYVEIGDKLAKEKGIKNGDRVKVSSIRGEAPCVAMVTKRFKPFTVEGKT
                     VHQVGMPFNYGWLFPKDSTSDSTNVLTPTVGDANVFCPEYKAFMVNVEKA"
BASE COUNT        10641 a         9669 c        10005 g        10582 t
ORIGIN      
        1 atcaaaagga acggctttta cgggaattcc cggttttact ttcaacatgc cttccacgat
       61 caccttatcg ccaggttcca gtcccgatga aacaagccat tcactgccga tggcgcggtc
      121 cagggttagc ggcttctgtt gaacttttcc ctccttatca accgtcagcg tcagaggatt
      181 ccccttggga tcgcgggaaa ccgcctgctg gggaaccaga atggcatttt tatggacgcc
      241 ttcttcaaca atggcccgga caaacatgcc cggcatcaat aaacctttcg gattaggaaa
      301 tacaatacgc aggatgaccg atccggtcgt cggatcaacg gtgatgtcct gaaattgcag
      361 tttgccctta aatggatatt ccgaaccgtc atccagaatg agtcgcacct gttgttgatt
      421 gctgtttctg gacagttgtc cttcctgaag tttacggcgc aatttcagaa tgtcagtcgt
      481 ggactgcgtg acatccacat acatcggatc gattttctga atggtggcca gcgccaaagg
      541 ctgatgcgcc gtgaccaggg cgccttccgt tatgccggat cgaccgatgc gaccggaaat
      601 gggtgcggtt atcgtcgtat attgaagatt aattcgggcg gtttcgagat ttgctttgcc
      661 gtattgaata tccgcctgcg tttgtttcag cgcggcggcg gcatcatcat aatcctgctg
      721 gctgacggct ttttcagcca gcaattcccg caggcgatcg gctcttaatt gaacggtcgt
      781 gagttgagct tcggaacggg ccagtgccgc cccggcattg cttaccgcgg cctgataaat
      841 tgccggatct atctgaaaca atggctgtcc cgcccggaca tcggcgcctt ccgtaaacag
      901 gcgtttctgg atgatgccgc caacctgcgg gcggacttcg gcgattaaat tagaggccgt
      961 ccggccggtt agttcggttg ttgtcacgac ctgtctggac tgaactgtca ccacggcaac
     1021 ttccgggctt ggcggagcca tggtttcctc ttttttgcca caaccgtcta cgatcagaca
     1081 actcagaaga atgatcaccg caatcaacgg cacaacttgc ttatttcttt tcatcaggca
     1141 cctctttcgt aatataaaac aatattgcat atttttgatt ttatcttttc ttcggcttcg
     1201 ggcgcggcgc gaaaccggtc atcagggttt gaatgtactt gcggatttca gcgctgacac
     1261 tggcttcatc gacttcattt tcagtccacc ggtcaagcat ggcggactga atgctgatca
     1321 tccaaaggca cttggcgagc acgccggtat cgatggcatc cgagagtctc cccagtctct
     1381 gttccgcaga cagatatact tccagtcctt caatggcgta tcgcggtccc atattccgtt
     1441 ctttgacgat ttcctgcaga cggacgcgta acttctgatc agcgaaaacc gtgttgataa
     1501 tcggtgttat ttcatagtgg gcatgataaa ctgaaagcaa aacattctcc agatttttgg
     1561 taagagtgct tttgcccacc tccagcggca gattctgcat atagtttttc gtttcacgta
     1621 cgcgggtttc gataacttca aagatcaact ccgccttgtc cttgaagtgg tgataaatta
     1681 aaccctcggc aacctttgcc tcgcgcgcaa ttgctctggt ggtaagtcgc gcgagaccat
     1741 gcgtttgcat aagccggtcc gtggcgttga tgatattttg acgattgctg atcattctgg
     1801 aattcctgaa atgtccatgg caaatgagac tgttaaaccc ataaaaatca tatctggcgc
     1861 tgagcgtttg atcaggaata ctaaacaggc acccaataat tgtcaagcat tttttgaacg
     1921 gatgtctgtc gagatccatc tataagtatt tttgcttcaa aattcagaaa aaataagacc
     1981 atgacggcac aaagttttca aaaaaactgt ttttgagttt gttgatttaa gcgtcaggaa
     2041 atatttcatg catcagcgcc agcgcttttt tcttttctct tttatataaa aagtaacaga
     2101 taaccgatgt cagaagagct cctaagctat tgacaatgag gtcggacatg gtatcgcgca
     2161 ggccgctgcc ctgaaacgcc cggccgataa gcgttgtatc gtggggtaaa tcccaagact
     2221 gcatggtggt tccgaaaaat acgtccatgg caaattcaaa aatttcccag atgaccccca
     2281 tggtcatggc aaacgtaaaa gcaaacacgg ccaccagaag cgggctgata tccatgctgt
     2341 atttactgtt tattttgtat atgatgatga atcctaagaa cccgataatg actccggaaa
     2401 gggtatgcag aacatcgtcc caccagaaga cattgtaata cagctcaaaa cggacactta
     2461 agaaaattcc ggcaaagata aagatcacga taatgatttc cagaatatgg ggaagatgaa
     2521 tatttttgta ttcgatataa tcgggcaaga atgtcaatgt cagtgtcaca accgaccaga
     2581 aaagcagttc aatcctttgg cttaattctt ctatgatttc ttttttggtg acgaggtcat
     2641 cgctcaagag atgcagggtg atgaaaacaa tatttgctaa tatcgaaatg atcagcaata
     2701 acctgattgc ccagataata taataatgat tctgattttc catttgtctg tttttctgat
     2761 tcatgtcctg ttgaatattt gccgatccca cgcggatgtg aaaaccaaaa atggtctgtt
     2821 tgggccgcag acgattattt tacggcattt ttgatggctt cttcgatgat tttggcatct
     2881 tccccgataa agggattaaa gatatcggtg tctatatctc ccttgattgt aaaattgtcg
     2941 tcatcaaact ttaccgttat cctgtaagtg ctgacgccga ctttacggct cattttcaac
     3001 aaaccatatt ccctgttttg ataatcaatt ttgtatgaca ttctggtgcc ttgattcatg
     3061 gcggctgaaa acattttgtc gtaatgattg ataaagacct gttgatcgta tttgaccgga
     3121 tttctgacaa tctgcgcgca agaaaacaaa aatagtatgg ctagaaataa gaaaaatttt
     3181 ttcatcataa tgttgtctcc cttgcttcaa tagaattttc agtcatctac tctggatcaa
     3241 cctttttatt tttagaacca ttatttttag tcacatccgt attcccgcaa aggatatcca
     3301 tgagtttctc cgcaggtgaa ccggcgggaa ttctgctcca ttttttaccg gatggcggca
     3361 aatcggaaat aagctttcct cttttgtcat aaataccgcc tgacagtatc ctgtattgcc
     3421 tggttgtgca gttgaattcg atccatttca tttcaaaatt taaaatgtag ggattgcgga
     3481 gatttttctc cgtttttgtc agccgggaga aattcctgaa ctttcccctt tcgttataga
     3541 taatcttcgt tctgacgtgc acaatattct tcgtaacttt tttaacgtcg cttggcttgt
     3601 aatattccgt gccattaggg tctgttccca gtaaagtcca gtttttcgcc caggaatcgg
     3661 cagccgataa aagaaaaaca gtcagaagaa tgatgatcag aaaattggta tttttcatcg
     3721 tttcttctcc cggaaaatgg aattcctgtt ttgtacctgt cattcgattt ttctatcata
     3781 gcactaatcc tattttttta aagaggaaaa tccggcgttt ttcttttatt gtggaaggca
     3841 ggcaacgcaa aaacagtttt tttaaaagag ggaaagataa attgattgtt gaagaagcat
     3901 ctttggtgag cggttgtttg tatcacagcc ggcgaccggc tgtgatacaa acaaggtttt
     3961 tgtaaaaaaa cgttttataa accttttttc tcaaaccagc ccaaaagccc taaaaaagcc
     4021 gcagccacaa tgataataac aaaccagtga ttgatcccca gtacggccgg cagggtgatt
     4081 tttccgaaat cgccccaggt gagaacggtt gttttcattg tcggatatag ttcggcgtaa
     4141 atgccggctc ccagaatcat tcccagtaaa ccaaagaccg catcatagcg gccttcgccc
     4201 agggcgccca ttgccgttcc gggacaatat cccaccactc cccagccaag gccgaatatc
     4261 agaccgccca atacgtttcc gccaagaatg agcggcttga tggagagttt gacaagcccg
     4321 agatcaaaaa gcagataaat ccccaccatg gcgaccagta tagtggtcag catgaattta
     4381 acaatagtca tatctttaaa acgcaaagcg cccagttggc ggtcataccg gatgacctgg
     4441 gctttctgca ttacaaagcc gaagagaatt cccgtgactg ccccgataac cagctcattc
     4501 atgatgattc cctccatata caatccgggc gataagaatg ccgcccagaa agaaacaaat
     4561 taatgaaaca taaccgctga aggctatctg agccagaccg ctcagtccgt gtccgctcgg
     4621 tcagccgtcg gcaagccggg cgccgaacat caggacgatg cctccaagaa atgcggcggt
     4681 ccaccgtttt gcgcgcgacg gaccgaatcg ttcttcccac atcttcggca cggcaaccat
     4741 gcgcttctct ccggatagcc aggcggcggc caaagcgccg aagaaaattc cgacaacaaa
     4801 catgaactgc cagtcgattt ttgctttgtt tttgatgaag tattccatgc cggcaatttt
     4861 atccggcgtt acggtctgtt cgacataacc ggcaacgcgg acaaaagttg ttgaggcgcc
     4921 aaaatacttc cctgtgatga aaaccgaaag gatgagcaaa agtcccgcca gggcgccggc
     4981 ggcatagggt gaccacgttt tttcacgaaa gacattcatc atctttactt ccttgagggt
     5041 tgagtcaaag cggattgcgt aaaaaaacaa aacttttcaa tacttataaa ggatttgata
     5101 ttcagtgtca atagctgaaa acgtccaatc agaacgtacg gcaatcaggt taaaaatttt
     5161 gatcccttct gaaaggaaca atgactgacc tgatcgttat aggttccgct caattgcccg
     5221 caggcggcca gaatatcact tcctttactg gcacgaagca tggttgtgta gtgggcgtca
     5281 atcaggattt tctgaaaagc ttccactgtt ttcggcgatg gcgaccggaa aggggagccc
     5341 ggatattcgt taaaaacgat cagattcagt ttacagcgct gtcctttcag caatgcagcc
     5401 agctttttag catcctgcac ggaagagttt atcccgtcca ttaaaatgta ttcgaaagtc
     5461 agcaatcgac gaccgggcat gggatattgc cggcaggcat ccagcaggat ttccaggggg
     5521 tattttttgt tgaccggcat tagcccgctt ctggtgtcat tatcatgagc gttgagcgat
     5581 acagccagat taatgcaaat atcttttccc agccggataa tctgcggggc aagaccgcag
     5641 gtagaaacgg tcactttgcg gttggaaaat cccagcccga agtcgccggt gatattttta
     5701 attgccgtga cgacattgtc gtaattggcc agcggttcgc ccatgcccat cataacgata
     5761 tttttgatgt ccgggcatcc gggcaattca tgtttcagag tcaggatctg tcccgtaatt
     5821 tccgaaggat tcaggttcct ttgaaagccc tggcgcgccg tcagacagaa tttacagtcc
     5881 atggcgcatc cggcctgggt ggatacgcag atcgtccagt tgtttttccc cgggatgaga
     5941 acgctttcaa taaaccgtcc gtcttccaga cgtaaaagcg ttttttttgt tttgtctttg
     6001 gattcctgaa ccttgacaat gtgcggacgg ctgatgcggg cgatttgaga aagagcggtg
     6061 cggaaatcgc gggacagggt ggtcatttca tcgaaagacg tgtagccgga ttgatacaga
     6121 catttcatga tctgctgtgc gcggaatttc tccttgccca gaacggaaat aaagtcctgc
     6181 aattcctcct gcgtaaaatc cagcagattg ggaagcgaat gttcagcgcg accggtcatg
     6241 gaagcttttc ttgtaatgtt gcggcaatag cgtcgataaa accttccgta ttcacttttt
     6301 tattggacgc ttttttctca gcgagcgcca gaagatcgcc ggtcatgatg ccgcgctcga
     6361 cggtggcaat ggcggcttct tccagtgtgt cggcaaacgt gacaacgtcc ggtgttccgt
     6421 ccatgtgacc gcgctggcgc aaaccgccgc tccaggcgaa aatgagcgcc atggagttgc
     6481 tggacgtttc ttcgcctttg agatgtttgt aataatgctt ctgtacnnnn nnnnnnnnnn
     6541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6601 nnnnnnnnnn nnnnnnnnnn nnnnnntgga gttgctggac gtttcttcgc ctttgagatg
     6661 tttgtaataa tgcttctgta cagtgccgtg agccgcctcg tactcgaacc aaccgtgggg
     6721 tgagaccagc accgatgtca tcatggccag cgaaccgcag gcggaagcga ccatgtccga
     6781 catgacgtcg ccgtcgtagt ttttcagcgc ccagagcatg ccgccctcgc ttttcataac
     6841 ccgcgccacg gcgtcgtcga tcagggtaaa gaaatattcg atacccgcct ggtcaaattt
     6901 attcttccag ttcttttcga attcctgctg gaaaatttcc cggaatccgg catcgtatat
     6961 tttggagatg gtgtctttgg tggaaaacca cagatcgatc ttttcgctga gggcgtaggt
     7021 gaaacaggcc cgggcaaaac tcaagatgga ttgttccaga ttatagttta cttgggcaat
     7081 gcctttgccg ggaaagtcaa ataccggaac gttgacggcc ttgccgccgt cctgcggcgt
     7141 aaagaccagt tcaagtttgc cggcggacgg aacatcaaat tccgtattgc tgtatacatc
     7201 gccgtaggcg tgacggccga tgatgatggg ttttttccag gaggaaacat tcggtttgat
     7261 attgttgacc agaatgggtt tccggaaaac cgtaccgtcc agcatggcgc ggatggtgcc
     7321 gttggggctt ttccatgctt ttttcaggcg gtattctttg acacgatctt cgttgggggt
     7381 gatggttgcg catttgatgc cgacgccgta tttcataatc gctttggctg catcgacggt
     7441 gactttgtcg tctgtatcgt cgcggtgttt tacatgcaga tcataatagt cgatatccat
     7501 gtccagatag gggaaaagga gtttgtcctt taccatttgc cacatcacgc gggtcatttc
     7561 atcgccgtcc atttcgacga cggtggtttt cactttaatt tttgctgcca taaaaaaata
     7621 ctgctccttc atgagaccca agcttcagaa caaagtgcgc tgccgcttgc ttattataat
     7681 gaaatgtgaa cttcaacagc ctggcgtatt gaagttttgg gttgaatgaa cttccgcagt
     7741 ctgccgcggg accttatgca ttattgatat tcagagctcc tccggccaga atgatctgtt
     7801 tctgcctttc ggacaaatcg caggtgacat aaaaagcggt cccctttgtt ttgtttctca
     7861 ttagaaattt tccgtccccg gcaatggtct cttttgtttc agtaatttca aggagatctt
     7921 ccatctcaat gatgtcgtaa tcgctctctg aacagaatgt cagcggtatg atgccgaagt
     7981 tgatcagatt ggccgtgtga attctttcaa aagatttggc aatgacgacc cgtactccca
     8041 gatacatcgg gcacagcgcg gcatgttcgc gggaagatcc ctggccgtaa gaatgaccgg
     8101 cgacgatgat attttgtttc ccgtcctgcc tgatggacat ggcccgcttg gcgaatgtcg
     8161 catcgacatt ttcgaaaaca aattccgaat atttcggaat attcgaacgg tatttcatcc
     8221 gcgcaccggc cggaatgatg tggtctgtcg taattttgtc accgaccttg atggttaccg
     8281 gtccgtgaat ggtatcggaa agttccggac tgcgcggcag aatgccgatg ttcggaccgc
     8341 ggatgacctc gatctccttc ttttttttgt ccggacgaat aatcattgtg tcatcaatgg
     8401 aaaacttctt cggcagcgtg actttcggat agcgcatctt cagatcgcgc ggatccgtga
     8461 atttcccggt tataaccgcc gctgcggcag tttccgggct gaccagataa acgttggcat
     8521 ccatcgtgcc ggagcgtccc tgaaaattac ggttggacgt gcgcaacgac acggcggctg
     8581 acggggggct ctggctgttg ccgatgcaga atccgcaggc attttccata aggcgtgctc
     8641 ccgaggagat cagatccgtc aggtaaccgt ttttggacag ctccttgagc acctgacgcg
     8701 aaccgggagc caaaataaag ctgacattgg gatgcacggt tttgcccttt aaaatttttg
     8761 ccacggtaac cagatcttta taagacgagt tggtgcagct tcccaggcaa acctgatgga
     8821 cgtcgatctt ctgcattttt ttaaccgtgc taatattgtc cggactgtgg ggcttggctg
     8881 ttaacggttc tattttggat aaatcgatat taacgacttt aacgtattta gcgtctttgt
     8941 cgggtttcaa ctcgacaaag tctttttctc tgtgctgtga tctcaggaag aggcgcgtca
     9001 ttttatcgct gggaaagacg gatgttgtta cgccgcattc ggcccccatg ttggcgatgg
     9061 ttgcgcgctc ggggatggtc aaagactcaa cgcccttgcc gccgtactca aataccgtgt
     9121 taacgttgcc ttttgtagaa aagatttcca gcaccttgag aataacgtcc tttgcggaaa
     9181 tccagggttt gagcttgccg gtgagtttta ttttaatcac tttcggacag accagataaa
     9241 acggctttcc ggccatcgcc agcgccacat ccagtcctcc ggcgccgatg gcgatcatgg
     9301 agagtgcccc gcaggtgggc gtgtggctgt ccgacccgat cagggtcttt ccgggtttgc
     9361 cgaaccgttc cagatggatc tgatggcaga tgccgttgcc cgcgcgggag aacacaatgc
     9421 cgtatttctg cgcgacagtc tgcagataca gttgatcgtc cgcattttca aagccgatct
     9481 gaatggtatt atgatcaacg taactgacgg acaactccgt ttttaccttg ggcatattca
     9541 tggattcaaa ctgcagatac gccatggtgc ctgtggcatc ctgagtcaag gtctgatcaa
     9601 tacgaattcc gatttcttcc ccggggataa gagtgcccga gaccaaatgc ttcaacagaa
     9661 ttttttctac caggttttgt gccatttttt attccttgaa gagaagaatt aatgttctaa
     9721 agcatttaca gccgcgaact tatataataa tcttaaccga taatcaactg tcaaaaggta
     9781 agaaatttta aaaaaataaa gggctttgca atcgttttac aaagccccgt tatttatgga
     9841 aatgaatgat ggataaaccc atttaaaaac aaaaggttat ccagattttc agcctccaac
     9901 cggcaactct tttatcctga cggcgcgttg aaatctttcc tgataaaagg gatgatccaa
     9961 cgagtcaatc cggacgcgtc tatttcttga agaggcatgg atgaattcat tatttcctat
    10021 gtaaattccg acatgtccga acgaacgatt cgtatgaaag aataccagat ctccttcgac
    10081 cagttcgtcg atatccacct ccacaccgac cttggattga tcacgggcaa cgcgcggcag
    10141 tttgacattg aaactgccat agactttttg aacgagtgcg gagcaatcta ttcctctttt
    10201 tgaatttccg cccagtctgt aaggcgctcc cagaaatccg gttgcctttt taatcagcct
    10261 ttgcatttca tccgtgcttt tccattttcc cagaaaatca tcctggtatc cgaaattaaa
    10321 gatattggag aaaaatcctt cttccccgtc ataattttct tccactgccg gatcttcatt
    10381 gacggcagca ttttctgtat cgtcaatgat atcttcatcc cgatcttccg caacgactgt
    10441 ttttacacgg gatgggggat cagaggccct taaaacaatt ttctggccgg cacgtatctt
    10501 tttggaatga atgttattca aagccattaa ttttttcagc ggaatgcggt ttttttgtgc
    10561 aatcttcgaa agggtgtccc cttttttgac gacgtaatnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnat gcggtttttt tgtgcaatct tcgaaagggt gtcccctttt
    10741 ttgacgacgt aatatgatga tatggtgttt tttgaaaccg tcgacttgga tgttcctttg
    10801 ggggaagtgg caatctttaa aacttgattg gctttaatgg ttgttttttt caggttgtta
    10861 gcttttttaa tgtcaatgac gctaacattg aattttttgg aaatactgta aagggtgtca
    10921 ccgttcttta ctttataatg tattgcaaaa gcgttactgc taagaccgaa caacaaaaaa
    10981 aggacaaaga gtcccgagaa tacagtcatc tgccgtttca taatgaaatg cctcctgttt
    11041 tatggggggt attatcctat taacggtgta catgtttcga tttattatca ttgctttaac
    11101 cgaaaaaact tataacctgt tgttgtgcac acctgttgtg catttttttt tgactcatgc
    11161 acaattaaca cataattttc tggactctta ctaaaaaaaa agccgtctgt caattttttt
    11221 tacagcagtg gatgatcttg taacagcaaa tagtgaattt ctatggcaaa aagtgttttg
    11281 cgccgacaaa gtaaattatt attggatttc aaaacgattt tcggttatgg cagtgcctgc
    11341 cgtcagggga tttccggctg aaaaccggag gtatcaaaag acgatggaag acggtaattt
    11401 ttttaagagg gctgttatgc gatttttcgg gaaaattatt tctattattc tggttctatt
    11461 tttagcatat tttattatcg atgtggggcg ctattttgtt tttccgtggg tggggtctct
    11521 ggcaaaaagc agtcctgaaa aaacggcatt tatgaagcac agagagaaaa tgtgggaaaa
    11581 agaaggcgtc aacaaaaaaa ttacgaaaat ctgggtgcct ctatccagga tatcgcctta
    11641 tgtcatgaaa gcggtcatta ttgccgaaga cgataaattc tggtctcacg aggggtttga
    11701 ttttgaggcc atgcagaaag ccctggaaaa agatattaag aaaaagaaat tcaaggccgg
    11761 cggcagcacg atttcgcagc aactggcaaa aaatctgtat ctctccccct caaaaaatcc
    11821 cattcggaaa gtcaaagaag ccattctgac ctggcggatg gaaaggaact tgagcaaaag
    11881 aaggattatg gagctttacc tgaatttggc agaatgggga gacggcgttt tcggtattga
    11941 agcggcggcc cggaaacatt tcggaaaaag cgccgccgcc ctgggacccc gggaagcggc
    12001 agttctggca gcgattctgc ccaatcccat ccgatatagt cctcacggtg gatcaaaata
    12061 tgttgcatac cgttcggaaa gaatttacca gatcatggtg cgccgcggaa ttgtcattcc
    12121 tgaatatgag gaagtgatct ctgaaacgac ggatgaaagc gaagaggaaa cggaagaaca
    12181 gatcgaggaa agtgttcccg aagtcaccga acaggaaagt gcacctgttc agcagacgga
    12241 ggaacaaaaa gaagaatccg ttcaggaaga acagaaagca actcctgttc ttcctgaaac
    12301 agaaaaaaaa tcaattccct gaggggaatc tctcttacaa tatttcgata gccgtggcca
    12361 tggaaacgcc tccgccgccg caaagcgtgg ccagtcccag cgtcttgccg tgtttgcgca
    12421 tggcatgaat taatgtaacc ataattcgag agccggtgga gccgacgggg tgacccagcc
    12481 cgatgccgct gccgttgata ttggtaatgt cacgttttaa gccgagttct ttttcgcaac
    12541 cgaggtactg cccggcaaac gcttcgttaa cttcacacaa ttcaaaagaa tcaagggtaa
    12601 gcttcggatc gtttttgagc aggtttttca cggctggaac cggacttaat cccatcacgg
    12661 aaggatggca accgccgcgt cctgcagaac gtattctggc gatcggtttc agtcccaatt
    12721 ccttggcttt ttccgccgac ataatgatca tggcggaagc gccgtcgttg agccccgagg
    12781 aattgccggc tgtcaccttg ccgactttgg gaataaatgc cggaggcagg gcttttaact
    12841 gatccatggt caaaccgggg cggaaatgtt catccttgtc gaaaatgatg ggcggcttgc
    12901 cttttttctg ggggatttca atcggtacga tctcctcttt gaaactgccg tctttggtgg
    12961 cgcgttcgac gttgttgtgg ctgcgcaggg cgacctcgtc catttcctct cggctgatgt
    13021 tcaaaagctg agcaatcagt tccgttgtat gacccatgat atagggtttg ccctttaaac
    13081 tttcaaaagg ctgacctgat ttgacggggc cgtcgtcgga cagcggcatg acgtaggaac
    13141 cgcaatgcag agcgcggatc atggaatcta tgaaagtagt atcctgaagg cggcagcccc
    13201 aacgggcatc aaaacttacg tagggtgtgc cggacatatg ctcgacgccg ccggccagga
    13261 taatatcggc cattccggct ttgatcatgg ctgcgccgct gaggacggct tccatgcccg
    13321 aaatacatac gcgattcatg gttacagcgg tcaccgattc cggaataccg gctaaaagag
    13381 ctcctacacg cgccacatta agtgtattac aggattcgag acaacagccg aaccggacat
    13441 catcaatgat tgacggttct atccctgccc gtttgatggc ttccttcatg acaaccgccg
    13501 cgatattagc accgatcaca ttctttaaag ttcctccgaa tgttcctatc gctgttctgc
    13561 acgccgaaac aattacaaca tccttcatcg ttcaaactcc ttatcgttat gattattttc
    13621 ttctataaaa tctgaatgac tcagacagac tttttaagaa gtaatttctt caactggaat
    13681 gtacaccttc aagcttttga agccattgat ctagcttatt ttctaaaaaa taaacaaaac
    13741 cgaccactcc atatcttata atccatctca aagtcaagac catcgatgtc ctgaaatgaa
    13801 aagtttgagc aaaatattgc attaagaaat atgatagtaa tttatgtaaa gtatggaaaa
    13861 ggtcttaatg aagggtgatt tgcgccgcaa taactttaaa atccttgatc ttttcagttg
    13921 accggccctg aaaaaatgat taataaggca gaaaaaagca attaaggagg tgacgccggg
    13981 aagcaaatta aagaaatcga aaagaattta ataaaaaacg tgcggtacaa aacatctgac
    14041 aaaattttaa aaagagtcca tattttaaaa atatgaaagg atgaacggct atgaataaga
    14101 aaattttttg tttggcggtt gcgttatgtg taagtgcatt ctttttgatt tcatgttccg
    14161 gcggaaaaaa cgcggccgac atcgcgatca aagcggcgga agaagccgtt aacgctacca
    14221 aggcggaggc agctaaagtt gttccggacg aagtcaagtc gctggaggat tcacttgccg
    14281 ctgtgaagga aaaatttgtt aaaggcgaat acaannnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14401 nnnnnnnnnn nnnncgctgg aggattcact tgccgctgtg aaggaaaaat ttgttaaagg
    14461 cgaatacaaa gccgctctgg aagaagcgac tgctctttcc gccagggcca aggaagtgct
    14521 ggatgcggca aaagcgaaga aggaagaact gaccgggaaa tggacggaaa tgagccaggg
    14581 gcttcccaag atgtttgaag atattcaagg caaggttgat ggcctttcca aacttaaaaa
    14641 acttccggcc agtctgacca aagagaagtt tgaagaggcg aaagccactc tggcctcggc
    14701 caaagacgaa tgggccaagg cccagacgag ctttgccggc ggcgatttca gcacggcagt
    14761 cagcatggcc accgtgttaa aagacaaggc tctgaagatt atggaaactc tgggaatgag
    14821 cgctcctgaa gcggctccgg caaaatagtc aacggattcg aatcggatgg aagggatatg
    14881 ctccccattt gtcagggagc atatcccttt tttatggaga tccttttgaa ggcatgaaat
    14941 cgtgcattgg aatcatcggg cagtctcagg tcggacagga tgtcgggttc tcatctcctg
    15001 ttgcgtgcag gacacacgaa cgtgatcatc cccgcggcag tcggcgggaa aatgcatttt
    15061 tatcggtttg ctttatcggt tttctaaatc ttctgaatat gaacacgccg acttttttaa
    15121 aagcaaaagc gataaataac acgatgatca ccagacaaac aacgggcaga agaatggcca
    15181 tgacggcaat gacgaccgcc ccgatcagtt ccagcgtagc gacgatgacg ttcccggttc
    15241 ccgcggtcgt catggaggat tttgtgcgca gagcgacggt cgagccttgc accagcccgg
    15301 ctataccgcc gccggcaatc agtgccaggg tccatttcag aaaaggcgac atgtccatac
    15361 tcatggacgc tgtggtgatg gttccggcga tgactgcggc aggtgtggcg atgatatcca
    15421 gaatatgatc aacgccgggt atgtaatagg caagcacctc gataagggtg gcacaagtaa
    15481 atgcaatggt tgcgtaggtg ccgccgatcc aggcaaattc cgccggcaac tgaatctgtc
    15541 cgcttaaggc ggccagattc atgatcaaca gcggaacaaa aatcctgaat ccgcatgccg
    15601 cactcaaccc gatgcccacc gctattccca gtatgatctc catgattatt cttgttaagg
    15661 tcctgtcata atgaatttaa atatctttta agcacctgtt ttcgggaagc gtttcaattt
    15721 ttctgaaaaa agcgtccatg gaatcggcca gtgatttgag cccgtcctga tcagagaggg
    15781 ggagcgtgtt catccctctt gtttccaaag cgtccatgac atcctgaatg gtcaggccgc
    15841 tgacgtcaat cgccggctga tgaccgcgcc tgtcttcccc ctcgatttcc acaacagaaa
    15901 agatccggct ttttatcaga tcgaaaagga tttcattaac gaaacgaatc gggattccca
    15961 actgactgga aatgtccagt gccgtcatgg gtttctctcc gcggatgaaa tttttgatga
    16021 cacgactcgt taccagaagc gaaagcagaa tccgcatgcg gtgactggcc tgaagaaagt
    16081 ccggttcgaa ttcataggta tctgcattct gataggcaac cgatacttcc gctccatata
    16141 aaacaatggt ccagctcagt tggagccaga ccagaaaaag cggcagcgcg gcaaaacttc
    16201 cgtagatggt attataatga acgacaccga tttgaaaggt tatgtaggcc cattgcacga
    16261 tctgaaaaat ggttccggca ataataccgg caacaaggcc ggaggaaaag cgaacccggg
    16321 cattcggaat aaaaatataa agaaaggtga aaagaaacca catcagcata tagggcagaa
    16381 gcttcaacaa aaagaagatg aaagaactga aacatcccag gacggtaaat ttttccagaa
    16441 tgacactcac ctgcgtcgtg atgaatacgg ttgtgctgct ggagagaatg agcaggacgg
    16501 gacaaataat gatcagagca agataatcgc cgaacatgcg gccgatcgac cgtttttctt
    16561 ttattttcca gatatcgttg aaagaagatt caatctgacc gagaagtttg aaaacagccc
    16621 acataagaac aaccagaccg acaccggcaa tgaggccacc ctgggttttt tccagcagag
    16681 aatgggaaaa cgcgatgata ttggcagcga tttcctcctg accggccagc ctgcttcgca
    16741 gctgtgtttc caaggcttct ttcagcccga agcctttcgc gataccgaaa gccatggcgg
    16801 caacgggaac gatcgaaatc agagcataaa aagtcagggc ggaagcgcgc agctgacatt
    16861 tatcctcatg aaaacctttg agggaaagaa tcagaaccct cgcttgtttg atcaggaagg
    16921 atttgccggc agaaagctgg gaacggcgaa ttctccagat gtcatgggcg atgaaattgg
    16981 cgagctgtcg gatctttttc ataatgtctt tcatgatggc gctccgcgaa tccggctgaa
    17041 agataatttt cagacaggaa gataagaata aagataacag atagatatgt taatcgcaaa
    17101 agatttttca tccgtgttga ggcagcataa gcccaaaggg ctcattttga ttaatgattt
    17161 taattgattt aattaaaaat tgaaaagtgc tattattgta aaacagccgt aaaaaaatta
    17221 acaggaccag ggagggaaaa catgcataaa atatttttca gaaagatgaa tgagaaatgg
    17281 aaaacatttt ttttccttgc cctcattttt gcaatcagtt cctgttatta ttatccacag
    17341 gagcaaatgg tgacccggcc gtctcctgat gaaaatatca gggctattac gcaaatttat
    17401 ttttatccca accgtaatca gtcggcggat ttgcaatcgc gcgatcacta tgcctgctat
    17461 acctgggcca tggaacaaac cggctttgac cccagcgtgt cctccattgt gccggaacag
    17521 cgtgtccgcg ttgttcccat gccgccgccg ggacaggata ccatagccat gtcgattgcc
    17581 ggagcggttc tgggtgcgtt aattgccgga ccgagacacg ccggcggcgg ggcattgatc
    17641 ggagcggccg gtggcgccat ggccggaatg gtttctgacg cttcccgggt tgaagcagcc
    17701 aggcaaatgg aagaggcgta caacaaccgt aatcagggac gcgatttccg cagggagaaa
    17761 aaaggcctgc agtttcggcg ggccatgtcc gcctgcatgg aaggtcgcgg atatacggta
    17821 aaatagtttt gcatgcataa ggaggttacg atgatttcag taaaattcaa ctttaaaaag
    17881 tgttttgtcg ttgtcctttc cttattcatg ttgacttttg tgagcgaatt ggttttcacc
    17941 cctgcatcac aggcgcagcc ttccaggaaa tcgtcggtac gtaccgtgaa gaaaggatcg
    18001 acagtacacc atcggaagca attctttgat gcgcgtcaca gccataatcg cgcgtatccc
    18061 gtccgcggtc attatttcag atcgcttccc cgcgatcacc gggtgatccg tcacggcagc
    18121 tctcgttatt actccacgtc tcacggcgtc tggtacagac cgtaccgcgg actctatgaa
    18181 gtgatcgctc cgccgatcgg aatttttgtg ccgtttttgc ctctttttta caccaccgtc
    18241 cgggtgcagg gcacaccgta ctactatgcg aatggtacct attacacgca gaccccggga
    18301 ggctacgtgg ttgttcatcc gcctcaggga gaggtcatgg aaaatcctcc ggtaacggac
    18361 gatgaagatg aaggtttcat ggatgacaaa ctctttatct atcccagcaa ggggcagagc
    18421 gaaaaacagc gtgatctgga ccggtatgag tgccatcagt gggcattgga gcaggccgac
    18481 tatgatccga ctcaaatttc ttcaggtcgt tctgccgatg aggtgatgca gggaagagta
    18541 gaatacagac gcgccattgc ggcctgtctg gaaagccgcg ggtatgccgt aaaataatcg
    18601 cttaaagcgg tcgaaaataa aattccccat aatccccttt ttcatgaaag gggcctgtca
    18661 ggcctggagg aattaacgat ggaaagacaa aaaattctgc agcagatgat cgatctgaat
    18721 aaaatatttt ttgacagttg gttctccatg atggcaatgt ttcagcacca gacggaagag
    18781 atcctgaaac cttttgtgga ttgtatgccg ggaatggatg gccggatgaa gaaattcatc
    18841 cggcattgga gcgaggaatg taaaaagaat cgtgaaacgt tcaaacgtga tatcgatgcc
    18901 ggctacgcaa aggcggaagc ttttttcgac tatcatgcca ttctcaagtt tcaggaacaa
    18961 aacgagcaac tgcttaatga ttttttgagt cagacaacct ggatgccgga cgatttcaaa
    19021 acggcggcgg aaaaaatggc agacgtctat aaagacggct gcgacaaatt cagacgatac
    19081 gtcgaaaaaa acatcaatgg cgtcagtgac ttttcatcct cgtccggcaa accgggaaga
    19141 aaggccggat cccgaaagaa ataaaaattc cccggacaat caaattaatg catcagccgt
    19201 tttgtgtgtg caattctaga aaaagacaaa ggtcatgacg ataaacagga ccaccaggac
    19261 ggggatggaa tatttgaaca tatatccaaa gaaactgggc attttgacgc cggcttcttc
    19321 cgcaatggat ttgaccatga aattcggcgc atttccgata taggtattgg cgcccataaa
    19381 aaccgcgcca acggaaatgg cggccagata aggaattttc tcaacaatca gcagcgcgac
    19441 tgccgcaggt tcgggcattc ccgggtagaa tttgcccagc atactgttga aaaaagtcag
    19501 gtacgtgggg gcattatcca gaaaactgga aaggattcca accgcccaga aatactgggc
    19561 aggggaatcg attgctttga tcagccagtg cagggcgcct ttttcgccgg cttttaaaat
    19621 ggccagcgcc gggatgatcg ttatgaaaat tccggcaaaa agatacgcca cttccagcat
    19681 cggagcccag ccgaaatcat tgtttttccg gacctctttg gccgtaaagc ccaacgatgc
    19741 cagccccatc agaatcagca cggcatcctt gattagattt tctgtggtct gatgaatccc
    19801 caggatattc acgtctccca ggcggacact gccgctgaat aacacagcgc cgataatgcc
    19861 cgccagaaaa atgaaattac gtccgccctc tatttttaaa ggttcgaatt cctcttcggg
    19921 gatcgcggtt ttattttctc ttttgtaata atgggagtcg agtgcaaaat aaatcagcag
    19981 cagcgtgacg ctggccacag ccatttccgg gaatatatgc atcgtccaga aaaaagggac
    20041 cccgtgcaga aatccgagga acaggggcgg atctcccagg ggggtgagcg aaccgccgat
    20101 attgctgacc agaaaaataa aaaaaacgat ggtatgcact ttatgaatgc gccaggcatt
    20161 ggaccgcaga atggggcgga tcaagagcat ggatgctccc gtggtgccga tgacggatgc
    20221 caggagggtg ccgataagca gcaccgttgt gttgacagcg ggagtgcctt ttaatgttcc
    20281 tctgacgtaa attcctccgg atacggtaaa taaggcccac agcagcagga taaagggaac
    20341 gtagtcgatc aaaatgatgt gggcgatttc atgaagcgct ttttctctga aaaaaaagat
    20401 aaaaggtacg gcaaaaacaa ttgcccaaaa ggccgacact ttcggaaaat gacggtgcca
    20461 gaaccgggga aggagcagag ggatcaacgc gatggaaagc agtatgccgg taaagggcaa
    20521 tgttgtccag agaggcagta gggtcccgat gtctgcggtt gaagagtcca gcccaaaacc
    20581 gcctgcaaaa acgacggcgg gaaaaaccac ggtcaagaat aatattaaag cacgatggac
    20641 aactttcatc atttttgttg tttttcgtcc tggataaaaa ccgtcctggt cgggtacgcg
    20701 aattgaatgc cttcgtcccg gaatctgcgg aatatttcca gattgatgga ttcctgaaca
    20761 tccatataag tgctgtaatc ggagctttgg acaaaatatg ccgcttcaaa gttaagcgat
    20821 gaatcgccgt attctttaaa atgaacccgg tcaaaacggg cggtttctgt tttgtcaata
    20881 atatccctga cgatcatttt aatcgtcttc aatttttcca ggggggtctg ataaacaacg
    20941 ccgaaattga aaataatacg tctttcggac atgcgccggt aattgcgaat gcggctcttt
    21001 aacagatcgt tgttggaaaa aatcagttgt tctccgctga ggctgcggac tctggtggtt
    21061 ttcagcccga tgtgttcgat ggttccggaa agcgaatcca cggtgatgaa atcgccgatg
    21121 acaaagggct tgtccaggac gatggagaga gaggccagca gatcgcccag aatattttgc
    21181 accgcaagcg ccacagcaac acccccgatg cccagccctg caaccaggcc ggtaatattg
    21241 acaccgaggt tgtcaagaat cagaagcagg atgacgatcc aaagaacaaa acgggcgaca
    21301 aatcccagaa aggtgatggt ggttttactc gaaaaatctt cttcggaacg ttttttgata
    21361 atattgccga tggcgaaggc gattgcacta ctggcccaga aacctgcctg taatatcaaa
    21421 gccagaataa cgaccttctg ccacaattga cccacggacg gtttgagcgt gatagtcagc
    21481 gaagccagat aagcggcagc aatgatcagg ataaatcctt tggtcctttt cagtaagccg
    21541 accagaagat cgtcgatctg attgcttgtg gataccgcca gacgtgaaag tttgcggacg
    21601 gcaaccgcct gaataagttt taaaataatg aatacaacaa ttaatatgga taaagcaatc
    21661 agccaggcct gtaaggtatt gcccaggtag gattcctgca ttaaattcat tttttctcct
    21721 tgtcagctat tgatgacggt tttgaatacg gcaatctcta acgccgatta tcggattttc
    21781 tgaaattact tttccggaat aactaaaaca ggcaggaaaa ctttatcaat cgtgtcgata
    21841 tccgtgctgt caaatagact cgcataaagc ctgccgcgat gcttatgacc gatgatgatc
    21901 aggtcaattt ggttgtcttg cgcatatttg acaatttctt ccgttgtttc tcccttggag
    21961 acggcataga tgatgtttat attttccaga agcgatgcat cgacggtttc cttgacccga
    22021 agagccacag cgtcttcgtg atcggtcggg tgtcgatacc ctgccagaat atcatttaca
    22081 tagaaaatat gaagagcgcc attcagtaac cgggcaaatt ccagcgctgt ttccagggaa
    22141 gaaatttttt cggagttcag atcaatggga tataaaatat ttttaaaatt gagcatatct
    22201 ttctctctga ataactgtac aactaatata atcgaaaata tatgtcaacc ttttctggtt
    22261 tgccgggaca gggaccatct ctgagaagag aagacattag aaaatgatga atttggtaat
    22321 attgttggcg agtgttcaat cccgctccgc tgtgctcaat gataatttcc aatcccactc
    22381 cgctgcgctt cggggataat tcgaccagca aacttcagtg cactgcgttt ctgaagtttg
    22441 ggtctcatta aaaagcctcc gtcgcctgat agactggcaa cggaggctgg agattcgtat
    22501 cagcagcagc tgtttcttat tctcccttga agtttgcttc tctcttttca aagaaggcgc
    22561 cgatgccttc ctgcgcgtcc ttggagttga agagttttcc gagctcttcg cgttcgatct
    22621 tcaggtgtat ttcagcggag atgctcgggc tgatgttgca cagcttggtg actgccgcga
    22681 cggcaagcgg agcccgtttt acgatcttgt ttgccagttc gagtgcggcg tccacaacat
    22741 ttccttcggt gatgtggtcg acaaggccga tagagagagc ttcatccgct tcacactgct
    22801 tgccgagata gatcatttcc agggctttcg atttgccgac gagccttggc aggcgcagac
    22861 ttccgccgta gccgggcatg atgcccagat tcgtctcggt caggcctatg cgagcgcctt
    22921 tcttcatgaa gcgtatatga caggccatag ccagttcgca cccgccgccc agggcgtgtc
    22981 cattgattgc cgcgatgacc ggtttcggaa aatattcaac tttgttccag atgtcctgac
    23041 cgcgtttcag gaagtcgacg gcgctgaaat caccgaaccc gctcgcaaga tcggcgcccg
    23101 cgctgaaggc cttttcgccc gcgccggtga tgatgacgca tcggatggct ttgtcggctt
    23161 caacggcgtc gagatgcttc cgcatggctt cgaaaacttc cccgctcaac tggttcatct
    23221 tcggtttgtc gatcgctaat atggctactc cgtctcttat ttcttttgtt actgcttcac
    23281 ccatgatgat cctccttgtg tgatattgtt tggttgtgct cggccggtta gccgggcgaa
    23341 ctggtaatca attatcttcc gatcattttg aacgagccgt taatcttgcc gcgcccggac
    23401 ttcaggaaat cgaaaccttc cctgaccttt tccccgtcgt gatgcagagt cgagccattc
    23461 cgcgtctcaa tacgtccggt cagagaaaac gattttcttt atgtcattga tgttgattac
    23521 agcgtggtcg agtataagag acaggtggtt gtgtgtcaag gggattttgg tccgttattg
    23581 tgaaactcaa atttcaaaaa cgaagggctt tgaaatttgc gggtcgaaca atcccgccgt
    23641 aactggaggg gaatgagatc cgagtttcag aaacagccgg tcctggaact tgcagattga
    23701 attatcctgc ataggtatgg ggataatact ccgcctttgg ggcggaagta tagtccagga
    23761 cttcccttgg agggctacgt gattttgctt gaaggggagg caaaaaaatg attacatcat
    23821 tcccacaaac aaaaaatcag gagggtcaag atggcttacg ataatattat cgtcgaaaca
    23881 aaagattttg taacggttgt cacactaaat cgtccgccga tgaattccat caatctgggc
    23941 attcgtgaag aattggatca gatcataacc gacgtacaga ccagcaagga gacccgggtt
    24001 gtcgttgtga ccggttccgg agaaaaggct ttttgcgcgg gaatggatgt tgccgatgtt
    24061 gccaacattg acaaaggacc caacggcata gacgtatgca ataaagtaga acgctcccta
    24121 aaacccgtta tcgccgtcat caacggccac gctctgggcg gaggatgtga gctggccatg
    24181 gcctgccatt ttcgatttat gacagacaat cccaaatccc agatcggatg tccggaattg
    24241 aatctggcgc tgacccccgg ctggggaggc atacagagaa tgcccagaat catcggcaaa
    24301 tccaaagctc tggattttat tttattcagc aaaaaagtca gcggtcccga cgctctggcg
    24361 atcggccttg tggataaggt tttccctgcg gccgatttaa tgaaggaggc catgcacttc
    24421 gcgcaggcgc tttccaaaag agcgcctata gccgtaaatg cagtacttaa cggaatgaac
    24481 atcggtttgg aaaaaggcat tgttgcgggt ctggaagccg acaagcagtg gatcgaaaaa
    24541 ctcaaaacca gtaaggatgc gacggaaggc gtggtcgctt tcattgaaaa acgcgaaccg
    24601 aattttgtag gcgaataaaa tccgattgac gtgcctcgcg gcggcatgtc tccgccgtcc
    24661 gcgggagatc ctgccgtttg cactgccgac gtcgctttac gctttgaccg gtgttattca
    24721 aatctgaatc tctccagaga tttttaagcc tccgttttct ttatacgaaa acggaggctt
    24781 gttattgatg atcctcaatg cgtatgcagg attttgcgat ttttgttcag cgcattgttc
    24841 gaggcgatct gttatgtcga ctttactgaa gcaaggggcc catgagctca aaaattccca
    24901 tcgaatcata caatggcttg gcgcctgtca gatcggaaat cttcgcaaag ttgtcgcgca
    24961 cttcttcagc gggaatatcg cgcgatccgt ctcccaggca tgcgccggtc gcacatacaa
    25021 tagccgtttt tgcgtaccag ccagccccca tcgtaaaaat ggagccgttt tcctggcatt
    25081 cttccgaaca gagataaacg accatgggga cattgaattt cgcctgcagc ttcggagcca
    25141 gctctttcgg aatcaggttc tccgtcatcc gggaataggc agtgggcgca atggcattga
    25201 cgtggacatt gaacttcgac gcttccagtt taaggacatt cataaaaccc accaggccca
    25261 tcttggcaga gccgtaatta atctggccga agtttccaaa gatccctgat gtggaagagg
    25321 tgaccatgat tcgtccgtaa ttgtgctcgc gcattaccgc caacgccggt tgagtcacac
    25381 agaacgctcc ccggagatgg acgctcatga ttgcgtccca ctcgtcggcg gtcattttca
    25441 tcatcgattt atctcttaaa aaaccggcat tgttcacaag aacgtcgacc gtgccgaacg
    25501 tatccactgc ggtcttgaca atgttaatgc ccccttcagg cgttgccacg ctgtcgtaat
    25561 tggcaaccgc ttttccgccg gagtttttta tttcggtaac caccgcatcg gcggcgtttt
    25621 ttgttgatcc cgatccgtcg cgggcaacgc ccaggtcgtt aacgacaacc gaagcgcccc
    25681 ttctggcaaa ttccagtgca tattcgcggc caagaccgga tccggcgccg gtcacaataa
    25741 cgactttacc ttcaaaactt atgttactca ttgcattctc ctttttattt tttctaaact
    25801 cttttgggta acttatgctt caggattata tactgttttg tgtaatttga ccggtttcat
    25861 ttgcttattg cgcatgcgca gattgagcat ttccacgccc acggaaaacg ccatggcaaa
    25921 gtaaatatac cctttgggca catggacgtc aaatccttcc gccaccaggg tcacgccgat
    25981 cagaagcagg aaagataaag ccagaatttt gatggtcggg tgggcgtcca caaatttgct
    26041 gatcggtttg gcggcaaaga gcatgacgcc gaccgcacaa atgatggcga tggccatgat
    26101 ggaaacgtcc tttgccagac cgaccgccgt gatgacggaa tcgagagaaa agacaatatc
    26161 cagcagggca atctgaatca gaaccgctgc cacactggat gaaataagtc tgggagtctc
    26221 ctcctgagcg ccctccaggc ttgtgtgaat ttcatgcgtg gccttggcca gcagaaagag
    26281 tcctccgccg atcagaatca tgtcccgtcc ggaaatttcc tggccgaata ccgtaaacca
    26341 gggcgcggtc agtttgataa cccagaagat ggaaaacaga agcgccagtc gtgtcaacat
    26401 ggccagcatc agccctgtgt ttcttgcaaa attctgtttg tttttcggca gccggctgac
    26461 cagaatagaa ataaagatga tattgtcgat gcccagaaca atttccaatg ccgtcagcgt
    26521 cgccagagcg atccacgctt ccggactggt tagccatgca aacatatttg tcctccgtta
    26581 attttaaaga tgaggtgttt aatattctat tcccgcgcgg tctttgatac cggcattgta
    26641 gtgatgcttg atttcctgaa cgtcgctgac ggtatcggcg agcttgatga tttccttcgg
    26701 tgcgtagcgg cccgttaaaa tcagatccac atgagcgggc ttgtttttta taagtttcat
    26761 cacgtctttt aaatcgacaa gtttaaaatc cagcgccaca ttgatttcgt cgagaatgac
    26821 catgtcgtat ttaccgctca taatggcctt gcgggcgata tccagtcctt ttcgggccat
    26881 gtcaatatcg atggccgcgg gatgatcgcg catgacaaaa ctgtccaggc cggacatttt
    26941 gatcgtgatg ttcgataaat atttttccgc ggcaataaat tcgccgtatt ttctcccctt
    27001 cataaattgc agaacaaaaa ccttatagcc ctggccgatg gcccgcaggg cctgtccgaa
    27061 cgcggaggtt gttttcccct tgccgttgcc ggtgatcacg atgaccaatc ccttttgttt
    27121 cgggacggca ttttccttcg atgtttttaa acccgaagat ttcttgctgc gcacgttgta
    27181 aacagcgggg tcttccgttt taaccatggt cattttttca cctgtttttg aaaaaatttt
    27241 tccgtgatgt cttccgccac ggtataggga tcggatttcc tgttctttat tttctcggac
    27301 agttttccca tctcgccgct gctgatcagg gattgcatca ggggttccat gaggcaggcg
    27361 ccgagcgcgt ctttaatttc ggccatgact ttgcgatgca ttctttcggt gagttggccg
    27421 ctttgttcca ggaaacaatg atgttcttca attttccgcg ccagttcggc ggtgttttcg
    27481 tcaaaggttg cccgttcaaa cgcatcgccc accaggacga tcggcggctt ccagccgtca
    27541 gcgtttttcg gggataaatt gagcatggaa gacagttctg tctgcaactg tccggttccg
    27601 gggcggttgg ccttgttgat gacaaagaca tccgctatct ccatcaatcc ggccttcatg
    27661 gtttgaatct cgtcgcccat cccgggaacc agaacgacca ccacgctgtg ggcgtgattg
    27721 ataatatcaa tttcctgctg gccgatgccg accgtctcga cgataatgat atccttgccc
    27781 atggcttcaa tcacatggat ggcgttgccg gcggcctgag agagccctcc cagagcgccg
    27841 cgggtggcca gcgaccggac aaaaacgccg tcatctgccg catggcgctg catgcggatc
    27901 cggtcgccca ggatggcgcc gccggtgaag gggctggtcg gatcgacggc cagcacgccg
    27961 acggttttgt tcagttttct gaattcggaa atcagcgcgt cggtgagcgt gcttttaccc
    28021 gcgccggggg cgccggtaat tccgataatg aaagatttgc cggtgtgggc gtagagtttt
    28081 ttgattttta ccttggcatc gggacgcttg tcttcgatgt ctctgatcag gcgcgaagcc
    28141 gcacgaacat cgcccgcgca gattttttta atggtggcat ccataaagct tcaagagaat
    28201 ttctagtgcg ttttgatatt ttttttaatc cattccacaa tggaatcgag tggtgttccg
    28261 ggagtgaaaa tcgctttgat gcccgattca taaagcatgg gaacgtcctg ttccggaatg
    28321 atgccgccgc cgatgacggc gatatccccg gcgccttttt cacgcagaag ttccacaacc
    28381 cgggggaaaa gggcgcgatg tgcgccggaa agacagctca atccgaccag gtcgacatct
    28441 tcctggatgg cggatgcggc gatttgttcc ggggtctgat ggcagccggt atagaccact
    28501 tcaaaaccgg catcgcggaa acagcgggca agaattctgg caccgcggtc gtgtccgtcg
    28561 aggcccggtt tggcaaccat tacgcgaatt tttctttccg acatattact atctccttaa
    28621 attatctcta ataccaagtt gcattcatca ggataccgcg gcggcgagga ggcaccggag
    28681 gcgcattact aatacgtcga ggacgccgac gacgatgcca acaaaggtag ccgtatgagg
    28741 gcggcttggt tttaatacag tcccgggtcc ctatattccc cgtaaacgtc gcgatagaca
    28801 tcgcatattt cctgcagggt tgccagattt ttcaccgcgt cgatgcagca aggcatgacg
    28861 ttattcccct ttttacaggc gtttctgacg tcttcaagag attttttcaa ggctttgctg
    28921 tcgcgcttgc ggcgcacact cttgagcctc ttgatttgtt cttcgcccac tttttcatca
    28981 atatcaacga gcggaagcgg gtgggtaacg tcggtggcat atttgttcac ccccaccatg
    29041 gatttctcgc ccgcatccaa ctgcttttga aacctgtaag ccgcgtcgga tatttccatc
    29101 tgcgggaagc ctttttcaat ggcggtaatc ataccgccca ttgcatcaat cttgtgaata
    29161 tagtcccacg ccttttcttc catttcgttg gtgagagact cgatgaaata agagccggcc
    29221 aggggatcaa tggtgttggc cacgccggtt tcttcggcca gaatctgctg cgtgcgcaac
    29281 gcgatttcca cggcatgctc ggaaggaaga cagagcactt catccagcga atttgtgtgc
    29341 aacgattgtg tgccgcccaa gaccgctgcc agagcttcca cggtggtccg gataacatta
    29401 ttgtacggct gttgagccgt caacgaacag ccggccgtct gcgtgtgaaa gcgcagccac
    29461 agggaacggg gatctttggc atgaaaacgg tcccgcataa tcttcgccca catgcggcgg
    29521 gccgcgcgca ttttgcatat ctcttcaaaa aaatcgatat gcgaattgaa aaagaaggac
    29581 agccgcgacg caaagtcgtc cacattcatt tttttacggc gaatcacgtc atccacatat
    29641 tcgatgccgt cacggatggt gaacgccagt tcctggacgg ccgtggctcc cgcttcgcgg
    29701 atgtgatagc cgctgatgct gatggtgttc cagcgcggaa catattttgt gccgaattcc
    29761 acggtgtcgc tgatgagttt gacggaaggt tccggggggc acatgaatgt tttctgtgcg
    29821 ataaattcct taagcatgtc gttttggatg gtgccgccga ttttggaaag cggtatcccc
    29881 ttgatatctg cagccgcgca gtacatggcc cataaaacgg ttgccggagg gttgatggtc
    29941 atggaggtgg tgactttatc catggggatg ccttccatga gggtcaggaa atcttccagg
    30001 gtgtcgatgg cgacgccgca tttgcccacc tcgcccagcg ctttgggcga gtcgctgtca
    30061 tagcccatca gtgtcggaaa atcgaaggcc gtactcaatc cggtttgccc ttcgcgcagc
    30121 agcatatgcc agcgtcgatt ggtatcctgg gcgctgccca tgccggaaaa catgcggaat
    30181 gtccagtaac gtccccgata gccggtaacc tgattcccgc gcaaataagg aaattcaccg
    30241 ggaacgccga tgtcttcggt ataattgatg tctttgatat cttccggggt ataaagcctt
    30301 ttaatttccg tgtccgacac ggtggaaaaa cgtttttttt gttcaggatt attttttaac
    30361 gtcttttgga cttccttttc ccatttctgg gtcagtttta aagacgcctc ctgcatttct
    30421 tcggagaaat acacgatcaa gtcctcccgt atggcgcgga atgattagga atattcgtaa
    30481 aaaaacttaa agccactaac tcaaattttt tgtttttgtc aagacatatt gaagcaattc
    30541 acagcaaacc acaggaaatc tttcattgtt ccgtgaatca aaaaagcacc cgcatcaatc
    30601 tctacagcgg gtgcttttgt tggcgaatcc tgtgtttata agattatatg ggtttaacgt
    30661 tttctgcttc caaccctttt ttgccttgct ggatatcgaa tgatacccgc tggccttcat
    30721 tcagcgtttt aaagccttcg acctgaatcg cgctgaaatg aacgaaaaca tctccgccct
    30781 catcctgttg gataaaaccg aaacccttgt tggcgttgaa ccactttact ttcccttctg
    30841 gcatagttgc acaaactcct ttcgtaaatt ttccaagtcg aattaacact atgacagaat
    30901 tgcaacctta aaacctttaa agataaatga gaatctctcc ggcaggataa atacacacta
    30961 tgctttaaag aatattacag attaaccttg tacttcaaaa tgtttaaatt caactttttt
    31021 ctcagcaaca tattgtttgc tgcagtggcc ctatttaatc ttaacatcca tcaaagtcaa
    31081 gctatatttc atgggattcg ccggagacgg gccaggcgac gtcggatgct taaaccgccg
    31141 cactttgagg gtttaagcaa gatcgtgctt aatatttttc ccggaatgtg ctattaaaaa
    31201 cacaattcaa gccgacaatg gattgtcggc caaaggagtg aatcatgaaa attaaagcgt
    31261 ccgtcctgat tgtactgctg gctgcgttgt ggatgacggt gtcggcagga gcggctgaag
    31321 tgaaaccccc tcaggtgggg caggggtttc ccgatgttga attgtccaaa ccggtgatcg
    31381 ccggtgaact gaaatatctc ggactgtccg gcagcggatc ttttagaatc aatcagatca
    31441 aggcggatgt agtgattgta gaaatattca gcatgtattg cccgcattgt caggcggaag
    31501 ctcccgccgt caacgagctt tatcagctca ttgaagaaaa tgcggcgctt aaaggaaaaa
    31561 ttaaaatcat cggaatcggc acgggcaact ccatctatga aaccgatatt ttcaagaaga
    31621 aatacagcgt gaagtttcct ctggttcccg atggagattt caagatccat aaaatcatgg
    31681 gagaggtcag aacgccctat ttcattgttg ttaaattaaa aggaaagaag gcggacgtga
    31741 tttattccaa agcgggcgct ttaggagata acaaaacttt cctgaaagag attattaaat
    31801 cagcgggatt gaaataggag gcggccatga aaaaaaagat cattttggca gcgttgttga
    31861 tttgttttct tgcacccggg gctttcgcct tgtccgatgt ttttaaaaat aatgtttatg
    31921 aaacaggcga actgaagcct caggacagtg tattgaaagt caaggttggc cagcccgcgc
    31981 ccgatttcac gctgaaagcc gtcagcggca aaacgatttc tctgaaagac taccgcggaa
    32041 agaaaaatgt tgtgatttcc tttgtgccgg ctgcctggac gccggtgtgt tccgatcaat
    32101 ggccgggcta caacatcgtg aaagacatct ttgacgtcaa tgacgccgtt ttactgggca
    32161 tttctgttga caacattccc acgctgtatt cctggacaaa ccagatgggc catttgtggt
    32221 ttgatgtcct gtctgatttc tggccgcacg gtgccgtggc ggatcaattc ggcgtactgc
    32281 gctccgacgg catttcggag agagccattt tgatcatcga taaaaaaagt atcatccgtt
    32341 acatcgatgt ccacaacatc aataagcgtc cgccgctgga gagcattgtc cgggaactgg
    32401 aaaaattaaa ttaatccata gaattatcat tttcaggata agccccgttt caacgcggat
    32461 gtttttcctt gacacatccc cgatattaat ttatgcacct tccaaatgtt tatgaacgcc
    32521 ggtcattttt tgtttggcct tgattttacg cgaatgattt gaaaaggtga tcaggttcga
    32581 cgtggaagaa aaaaaaagaa acaggaaacg aaaagaaaag gaaaatagac gcagtacgat
    32641 actcaaagcc gcccgaaaac tctttttaga aaggggtttc aaagctgtta cggttgacag
    32701 tattgctgaa aaggcggaag tcagcaaggg atccatttat ctttgctttg aaagcaaaga
    32761 agaaatatac acacagattt taattgccga caacatttcc ctcaacgaac gcattcagaa
    32821 tttttccctg attgaagcct cggcctccca gcttctgctg gagtttgccc gtatttatgt
    32881 cgatttcttt ctcagcgaca aggaactttt cagaattctg atgacgttca tgatgcaaac
    32941 ggggcagatg cacttgacgc agcagcaaag cgatgagctg atccggtcga cgaatgaaaa
    33001 catcaaagtt atttcggaaa ttattcaaaa aggagtcgat tccggtgaat tcagaccgct
    33061 ggacaatatc ttgcagatgc agaacgccat ctggggaatg ttgaacggtg ttatttcact
    33121 tttccttttc accggcaatc cgatcaaaag aatggagaga attcactcta ccgttcagga
    33181 aagcatgaat gttttcatta gggggttgaa ggcgtaattt ttccggaatg caaggtgcgc
    33241 aatccgaatg ccttgcggtt cagtgcaggg cttgaaccgg gtatttcggg tttacttccc
    33301 catggtttga aataattttt taaattcctg ctcggcctct tttaatatgt tttccacatc
    33361 tttttccagt ttctcgaatc tttcgataat ggttctcgcc tgagccgtca gttgcagggc
    33421 tttttctttt tcgctggttt ccaccagttt tttttccatg cgttcttccg acgctttcag
    33481 tctgccccag gccgcacggt atgacattcc cagttccttg gcggcggcat tgaggctgtg
    33541 ctgctgttcg atggatttga gaatttcatc gcggcctttg ccgaaaatga ccttgccgtt
    33601 ttcttcaatc cagattttat atttgatttc catcggtgcc tcccccccag gctattgaat
    33661 cttgttttgt tataattaac atacttacaa tatgtcaata aaattcgcaa ccgccacagc
    33721 gccgtaaaac gtaaactgtg aaccggttga agaattggag aagaagacag atgctctcag
    33781 gccggcataa atcatcttga ctattcaatg acgttgcctg tatttacata aaaaaatcac
    33841 tgaaggagtt gatcgtatgc ccggactggt tcgatttggg gtatcgctgg aagaagattt
    33901 gctggttaaa ttcgaccggc atatcaagca gaagaaatac accaaccgtt ccgaagccat
    33961 ccgcgacatg atccgcgagg aactggtccg gaaagaatgg acggaaaaca aggaagtgac
    34021 cggcgcaatc acgctggttt atgatcatca caccagggaa ttagtcacca aagtgcttga
    34081 tgtgcagcat gattatcata cctgcattct ttccacgcag catattcatc tcgatcatca
    34141 taattgtttt gaaattgttg tgacccgggg caagtccaaa gaaattgagg agctctttca
    34201 gaaactcaaa tcgatcaaaa gcgtcaaaca tgccggattc atgatggcaa ccaaggggaa
    34261 ggatttaccc tgatgcgttt aaaggggtta tcgcttctgc atggataagg gcagttccag
    34321 attattttct tccagcagtt cctgatgggt gaggatctcc gaaaccgggc cgtcggcggc
    34381 cacccttcct tctttaatga caatacaccg atgacagaca tccagaatca gatccagatc
    34441 atgtgaggcg atgatcttcg aatgctcgaa ggttttcagc agattgatca gcaggcgccg
    34501 cgaccgggga tcgagattgg ccgccggttc gtccatggcc agaatatccg gctccatggc
    34561 catgacggac gcaatggcga cggcgctctt ctggccgcag gagagatgat gaggaggttt
    34621 gtttttcagg tccaggctgt tgaccagatg cagggcgaat tccacccgtt cgcgcacggc
    34681 agtatcatcc aaccccagat tgagcggtcc gaaagccacg tcttcgtaaa cagtcggcat
    34741 aaagagctga tcatcgggat tctgaaaaat gataccgacc tttctgcgga tttccggttg
    34801 cgtcttcttc gtcagataaa catcgccgat attcaccgtg cccgatgtcg gcatcaggta
    34861 gccgttgagg tgcatcagca gcgtagattt gcccgcaccg ttggcgccga tgattcccac
    34921 cgactccccg tgcgtgatcc ggaaagaaat atcctggagg gcttgggttc cgtccggata
    34981 gcggaaagaa acatttttaa actcgacgat atggtgactc atgaaaaaac tccttgtgca
    35041 aaccggccca gaatttcggt tgccggacag aaccggaaga tcaacagaaa cgccgcaacc
    35101 gtcgcggcaa aaatcatgtc gccggttttg aactgaaaat gtttgagcgc gggcatatct
    35161 ccctgaaagc cccgggacaa catggcgtta tagacccgtt ccgcgcgctg caccgtccgc
    35221 aaaaaaagcg ttcccgcaat gcggacaaag attttgatgc ccttgcctcg gtctccgaaa
    35281 gagcgcatat ttctggcccg gacaatccgc atggtttcct ccatcaggac aaaaatatag
    35341 cgataaagaa aaagaagctg tgaaacaaaa accgaaggca gccccaactg cctcaacgcg
    35401 tggcagacac cgggaaaaga ggtggtggcg acaagcagca gagccgtgct gacggtaagc
    35461 gtgaacttga gcaggatgga aataaaagac agccatcctg ccgaaatcgt atatccgttg
    35521 actatcgata tctgtcccga atccagcagc ggattgaaga tgccgataaa aacggcgaaa
    35581 ggtgaaacga gaaggacttt tttaaagata aacataaacg gaatctcgcc cagcgtcatc
    35641 agaagcaccg ggaaaagaaa aaaaggcagc agagcaaaaa tttcgtattt ggaaaaggaa
    35701 atgaccgtaa gcagaaaaag cagggtaaca catagtttga cccgtggatc cagccggtgc
    35761 acaaaggtgc tcttgtagga aagctgatcg aggaagccga tattatagta ttgaatgtca
    35821 aatgacatga tttggcccga taatggcttt aagaattgat tctagatatc atacgccctg
    35881 acaggaaatc cagctatttt caaggcgtat gccgggtatg atgacaaaag ttattgtttt
    35941 ttaaaaagcc gtatgccgaa accgatgaaa atgatgaaca tcaacacaat ggccgatccg
    36001 acaactccgg cgacagatgt tccgggttca atgcccggcc agaccgaggg cgactcctcc
    36061 gtgttttgtt cgtccgcggg cggtttgaaa ccataatctg ggagaaatgc cgtcttttcc
    36121 tgaatatctt tgaggacagg cgcaatgccc tgtgttgcct cttccagctc gcccttgccg
    36181 gtaactttct caatggacca ctccagcccg tcgggatgcg ttgaggcaaa ccaggaaagc
    36241 gcaccgccgg taatgaccgc cagaatcaca aatgctgcaa gaatgttctt gatggaaaga
    36301 ccggcatcca ggggacgggc ggcggcatgg gcatgcagaa tttccggccg ggcctgttgc
    36361 acatacagga tgatgccggc cgtgatgatt ccctccacca ggccgatggc cagatgaatc
    36421 ggctgcatca gcaggacaaa cgtgccgaag gggagttcgc tgattccgga gaatagcgtt
    36481 tccagaacca ccgaaaaagc gcccaactgc agggcaacga ttgcgccaag tattgcggca
    36541 acggataccc gtctggcgct ttttccgtct ccggcaacag gtttgtaaat caggggataa
    36601 accagaaagc agggatagat gcccatgttc cagatattac agccaagcgc gagtagcccg
    36661 ccgtcggcaa agaacagcgc ctgaaccgtc aatatcgagg ccatggccag aaaagcggcg
    36721 tagggaccca gaagaacagc caaaagcata ccgcctccga tatgtccgct ggaacctgtg
    36781 acgggaatgg tgaagttgat catctgcgcc gcaaagacaa acgcgcccat gacgcccatc
    36841 agcgggatgg ttttgttatc gatattttct ttgagctttt ttgatgaata tccgatggcg
    36901 gccacggaac cggcccacat cgttgctccc accgcgggtg agagtaaagc atccgccata
    36961 tgcatgttaa attctccttt taaaaaagat cagttatccg ccttcctctc tagaaccgga
    37021 atgcaagtcc ggcgagcgca gaaatatcgg cttccgagtc tgtcaaaccg tatttcacgc
    37081 cgaggtcgat gtccaaattt tctgcgatcg aataaataat cccgccgata agaaacgcag
    37141 gatcgtcatc cgcttcgcca tcaggattgc gctcaacgcc gatattggcg accagactta
    37201 agtctttgac aatttcccag gcagcggcaa cggaggcatg ccagatattc tcgcgctcgt
    37261 cggcgtcgtt ttcatttctg atgtatccca gattaacatg aaacgcgaac ggttccactt
    37321 cctgggatgc aatgaaaaaa atctgacctc caaattttcc tgtgcccagt cccttgtcat
    37381 catcgcccgt ggggaaactg attcccggtt tcaacgccag gctgaagcct tctttttcaa
    37441 agaccttgat ttttgcttct aatgaagcat cgccgatgcc gtcttcctta tatacaaggc
    37501 tgccattttc tttttctctt ccccaaagat aaggcaggct taataccaga tcaatattat
    37561 ctatcatccc gtaagacaat gtggctgccg tctcaactcc cgttgcttta atcgaaacac
    37621 caccgacatt ttctttgtcc tgatcatatt gtccggtcaa ttccaattga aaatttccct
    37681 tcccctgcgt gccggtgtcg tcggtaatca ggggatgcgc cgcccaggat ggaccggcga
    37741 tgaaaaacag cactgcccat accaatccgt ttataaaatg ttttctgatc attttctcct
    37801 tccatttgtg ctacgattat aattttagtg gcacgatatg gcggttctgt caagctttta
    37861 atgattttaa aaagcaataa accctattaa accggcttca ttaaaatatg ttttatataa
    37921 catattgact tttgtatgac tttcggtcaa tctatgccac gtttgacata taccaaaact
    37981 gacataggta gtggggagga ttttatgcat atttcaagaa gaggtttttt aactctttcc
    38041 agtgccgtcg gaggcgggat tgccctgtcc agcttggggc ttaatctgaa gccgacgcgg
    38101 gcatatgcgg acgagttaga caagatgaac agagtgaagg ccgccaaaca gacgacgacg
    38161 gtttgctgtt actgttccgt cggctgcggt ctggtttgca gcacggacaa ggcgaccgga
    38221 aaaatcatta acattgaagg cgacgccgag catcccatca atgaaggatc gctttgcgcc
    38281 aaaggtgccg gtttcttcga tctgaccgaa gccaacaagc acaggctaaa aaaggttctc
    38341 tatcgcaagc cgtatggaac ggaatgggaa gagaaagact ggaactgggc gctgcccagg
    38401 attgcccgct tgattaaaga cactcgtgac aaaggttttg taacgaagaa cgccaagggc
    38461 gaactggtca atcgctgcga aacaatcggc cactacggca gttccaacgt agataatgaa
    38521 gaatgttggc tgatgagcgt caaatgccgt gctctgggtt tggtttatat cgatcaccag
    38581 gccagggtct gacacagccc atctgtagcg gctctgggag agtcgttcgg acgcggttcc
    38641 atgacgaacc actactgtga tttgaaaaac gcggatgtca ttttgattat gggcagcaat
    38701 gccgcggaac atcaccccat cgctttcaag tggattatga gagcaaaaga aaagggagcg
    38761 acggttatcc atgtcgatcc ccgttataca agaacatcgg cgcgctgcga tttccatgtg
    38821 ccgcttcgct ccggtaccga tatcgctttt ctgggcggta tgatcaaata tattctggaa
    38881 aacaataaat acttcaaaga ctacgtagtc aattacacca acgcgtcgtt cctcgtaaac
    38941 aaagattttt cttttaatga cggtcttttc tccggttacg attcgcaaaa acgcaagtac
    39001 aataaagata catggactct cgataaagat gcaaacggta ttcccaaacg ggacatgacc
    39061 ctgtctgatc cccgctgtgt tttccagatg ctgaaaaaac attattcacg gtataccctg
    39121 gataaggttt ccagcatcac cggcgtatcc aaggacaacc tcctcaaggt gtacaaagcc
    39181 tacagcgcga ccggcgctcc ggacaaagcc ggtacggaat gctacgctct ggggtggaca
    39241 catcataccg tgggcgtaca gaacatccgc tccatggcga ttattcagtt gcttttgggc
    39301 aatatgggta tcgcaggcgg cggcatcaat gcgctgcgcg gcgaacccaa cgttcagggc
    39361 tcgacagacc attgcatttt atatgcaaat ctgcccggtt atctgaagat gcccgcggct
    39421 tctctggaca cgctggaaca ctatttgaaa aaatgcacgc cggaatccaa ggatccgcag
    39481 tcggccaatt actattctaa ttaccccaaa ttctttgtca gtttcttaaa atccctgtgg
    39541 gacgacaagg tgacaaaaga aaatgatttc ggctacgact ggcttcccaa aattgatgac
    39601 gggaaacact attccacgat gcacatgttc gatgcgatgt atgaaggcaa gatcaaggga
    39661 tacttctgtg ttggcgcgga tacggccgtc agcacgccga acagcaacaa ggttcgcaag
    39721 gcgatggaaa atctggactg gctcgtgggt gaaaatattt tcaacaacga aacctacgaa
    39781 ttctggagag gccccggtgt cgatcctgca aagatcaaga cggaatgctt cctgcttccc
    39841 gcttccgcga gtatggaaaa ggaaggatcg cagagcaatt ccggccgctg ggttcagtgg
    39901 aaatacaaag ctgccgaagc tcccggcgat gccattcccg tgggtgaaat cgaaattaag
    39961 attatggctg aagtcaaaaa gctttaccag aaagaaggcg gtcccaatgc ctcggctatt
    40021 accaatctca agtgggatta tctggacagc aaggggcaca tggatgtgtt gaaagtttcc
    40081 catcaggtca atggcctctt tacaaaagac acggttatcg aggataaggc gaaaggtaca
    40141 tccactttgt ttaaaaaagg acagtgtgtc ccgacctttg gaaacctcaa ggatgacgga
    40201 tccacagcct gcggtaactg gattatggcc ggtagttttg acgaaaaagg tttcaacaaa
    40261 atgaccaaac gcggcaaaga cgatcccacc ggtttggggc ttttccccaa ctggtcattc
    40321 gcctggccgg tcaatcgccg catcatttac aatcgcgctt cctgcgatat ttacggcaaa
    40381 ccgtacaatc ccaaacgtaa tattctggaa tgggtgggag ataaatgggt tggagacgtg
    40441 cctgatggtc cctggccgcc gcaagcggac aaggcaaaag gcaaatatcc ttttattatg
    40501 aaacaagatg gtgtggcatc tttgttcgga cccgccatgg ctgaaggtcc tttcccggag
    40561 cattacgaac cgctggaaag ccctctggct aaaaatccga tgtccggaca gctcaacaac
    40621 ccggccattg aaatcttcaa aggtgaaatg gataaagtgg ccagcgccag cgagaagttc
    40681 ccctatgtct gcacgacgta ttcctgcacc gagcactggt gcaccggcgc gctgacccgt
    40741 tggcaggcat ggcttctgga aatgcagccg gagctctacg ttgaaatcgg cgataaactg
    40801 gctaaggaaa aaggtattaa aaatggcgac cgcgttaagg tttcctccat tcgtggcgaa
    40861 gcgccctgcg tggccatggt gaccaaacgc ttcaagcctt ttacggttga aggaaaaacg
    40921 gtccatcagg tcggcatgcc ctttaattac ggctggctct tccccaagga cagcaccagt
    40981 gacagcacga atgtattaac cccaaccgtt ggtgacgcga atgtcttctg tcccgagtac
    41041 aaggcattca tggtcaacgt ggaaaaggct taaggaggat aaaccatggg aaatgaactt
    41101 gtaaaattga ttgatacaac cctgtgcacc gcctgccgcg gttgccaggt cgcctgcaag
    41161 cagtggaatg aaatgccggg cttcaggaca aaacaaa
//
LOCUS       DAOOLB010000014        40564 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_723_length_40564_cov_39.080822, whole genome shotgun sequence.
ACCESSION   DAOOLB010000014 DAOOLB010000000
VERSION     DAOOLB010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 40564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40564
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_723_length_40564_cov_39.080822"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PLF77_03340"
     CDS             complement(<1..586)
                     /locus_tag="PLF77_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC07920.1"
                     /translation="MKETGRNQALKEIKELKNKIKELENLLNTTKVGQTLMSTGMVYR
                     TIFRMSPNTIVVSKLEDGTIYDVSDSFCEKSGFARKQVIGKRASTLGIWLDQDREEIK
                     KRLLRNGRYRNMEVRHQVKGGKMLQCLQSAELITIDDERYIITVVVDITERKMMEERL
                     RENEEKYRQLFDNSPAGIYQIDYQTGRFLKANDVF"
     gene            complement(756..1340)
                     /locus_tag="PLF77_03345"
     CDS             complement(756..1340)
                     /locus_tag="PLF77_03345"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPC07921.1"
                     /translation="MHIPPVFVYPEAVLFWTALIGSFYLEIKFAGALARNPSNTQDAG
                     TRLLIKIGYRVAFVLALMASLTPWFVITWQRPALAVGTGMLIAGSIFRQYSIRALGKY
                     FTPEVVVKADQPVIEEGPYRWIRHPAYSGAFIMFAGIGMALGNWLSFAVLFLENCFVY
                     SLRVKAEEKALIDTIGGPYRVYMTRTKRFIPFIF"
     gene            complement(1422..2318)
                     /locus_tag="PLF77_03350"
     CDS             complement(1422..2318)
                     /locus_tag="PLF77_03350"
                     /inference="COORDINATES: protein motif:HMM:NF021287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HPC07922.1"
                     /translation="MNDNLIFQETPDLINPYILIGYRGWLNAGEMATGSIDYLRRKLD
                     ARKFASIDIRNFYIWQVPGFDPAQVMRPHAVVERGLIKSLNEPANEFYFWKSGKENDL
                     ILFSGFEPNVAWPEFTSAVLNIAKRYQACRIYVLGGVFDQVPHTRETIVYAVLSRPEM
                     KHEFQSFPLLNYTGPCSFSTLLVNHAGREGIEAASIVARVPPYIETFNAKIGCEYLKK
                     VLSRTSLNIDLSDLKKSGDAVNKMMENDFRQNLEALVQLRQLEQMYDESLMQGYGENP
                     EVDIDELMQAMHEMKKDIPKKH"
     gene            2513..2782
                     /locus_tag="PLF77_03355"
     CDS             2513..2782
                     /locus_tag="PLF77_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPC07923.1"
                     /translation="MVFLPEGRYYGSLYRSFLCREINNLLVTGRAISMDRGALASARV
                     MGPCLAEGQAAGTACALALSSGQSIDAVDTGKLREMLAKDGARLK"
     gene            2997..3455
                     /locus_tag="PLF77_03360"
     CDS             2997..3455
                     /locus_tag="PLF77_03360"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPC07924.1"
                     /translation="MPAKKLKEFLDSHGVKYVKLTHSTAYTAHEIAQSLHISGRKVAK
                     TVIVMIDGKMAMAVLPGNRSVNFDHFQKAVGAKKIELATEAQFKGMFPDCELGAMPPF
                     GNLYGMEVYADQHLTDDDEIFFNACSHSELVEMSYPDYERLVKPNVVKLT"
     gene            3486..3731
                     /locus_tag="PLF77_03365"
     CDS             3486..3731
                     /locus_tag="PLF77_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07925.1"
                     /translation="MNGKYQEIQVECYSGFKANERPVAFTYQGERQEIQEVVDRWYHG
                     GLYSSKPVINFFKVRTADGKVYLLRYQFDSDAWSLRI"
     gene            complement(3773..5293)
                     /locus_tag="PLF77_03370"
     CDS             complement(3773..5293)
                     /locus_tag="PLF77_03370"
                     /inference="COORDINATES: protein motif:HMM:NF036848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HPC07926.1"
                     /translation="MNSENIPVIIGAGQVTKREASSVQGGSPIDLMAAAVKIAAEDGG
                     VTEKYLQQADLLMTTSLFSDDGIANPPGCVADKLNLTDARCRVSGFGGVMPHAVLHHA
                     MQEIAGGKAGLVILTGAEAQHTMQQAKNSKTFTGWNVASGRHCLPPLTPLGLFDGACE
                     TEHIHGLSLPAYVYPMFENALRRRYGCTIEEHAEKIGRLMSRLSLVAAKNPLAWFQHS
                     FTPEEIMTLSETNRNTAFPYTKRMNAMLLVNQAAALLVASEAQAQKMGVNPLKWVYVH
                     GYAEANDHWNVLEREGYGFSPAIEIAGRTALKQAGLTIEDIDFFDLYSCFPVAVQVTR
                     DMLGIPENDSRDLTVTGGLPYFGGPGNNYVMHSMAQMVEVLRRHPGRTGLVTGNSFYM
                     TKHSTAVCSTRSPENNAAATADTRTCQQAVDKRPKYEIDPTPSGRATVDTYTVIYDRD
                     NLANKGIVIGKEENGKRFAAFTPSDPSLFSAMIEKDFCGVTGRVVSKDKINLFTPD"
     gene            complement(5308..5625)
                     /locus_tag="PLF77_03375"
     CDS             complement(5308..5625)
                     /locus_tag="PLF77_03375"
                     /inference="COORDINATES: protein motif:HMM:NF022815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HPC07927.1"
                     /translation="MKKATKESWTQKVKETSFALDLQSGVFTFDNPRRIAESLKESAE
                     SSTQRKAEPFQSAMSMLNYYINRAGTKLPAERKAILEQAKDELRVLFGRGNKSKTSLK
                     IKK"
     gene            complement(5692..7239)
                     /gene="putP"
                     /locus_tag="PLF77_03380"
     CDS             complement(5692..7239)
                     /gene="putP"
                     /locus_tag="PLF77_03380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HPC07928.1"
                     /translation="MTGNFNFYMLMSIAAYMVVVIVIGFVFARRNDQIGDFYLGGRKL
                     GTIVTAMSAEASDMSSWLLMGLPGVAYLSGIADAGWTAIGLAIGTYVNWLTVARRLRC
                     YSQLSQNAITLPEFFSNRYRDSKKILTWIASIVILIFFIPYTAAGFASCGKLFNSVFG
                     LDYTTAMLIGAVVVVAYTTVGGFLAASTTDLIQGIVMSIALLSILMFGAAVAGGIDAV
                     WQKADAIPGYLSMMFSHNSTTGQTERYGLITIISTMAWGLGYFGMPHILLRFMAIRDE
                     KKIVFSRQIAVTWVVVSLAVAVLIGIVGQTLSLNGEIAALEGSASETIIIRMAQLLSH
                     TGVISSLIAGVVLSGILAATMSTSDSQLLVASSSVTENLIKECFGIRLSEKTGMMIAR
                     SAVIVIAAIAAFIARDPDSSIFVIISFAWAGFGAAFGPVMLFALFWRRSNVYGAIAGM
                     VSGGVMVFVWKYLVRPLGGVWNIYELLPAFVFACLAIIVVSLLSKAPAREITDEFDAV
                     VKMGNRK"
     gene            7473..8141
                     /locus_tag="PLF77_03385"
     CDS             7473..8141
                     /locus_tag="PLF77_03385"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPC07929.1"
                     /translation="MLDVIEKALKEGRKTLSEYESRQVIESAGVAVAVAALTKTKEEA
                     IREAEQMGYPVAMKGCSAELSHKTEAGMVALNVNSAQDVAQVFDALTGKVKNLDGVLV
                     EKMVRGSRELVIGLSQDSSFGPCVMFGLGGIFTEALKDVTFRVAPLTKQDALEMIDEI
                     KTKKLLGEFRGSPAVDRNALAKALVGIGDLAIKYEAIAEIDINPLIVCGDKPVAVDAL
                     VVLK"
     gene            8273..9664
                     /locus_tag="PLF77_03390"
     CDS             8273..9664
                     /locus_tag="PLF77_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPC07930.1"
                     /translation="MLDYFFNPKSVAIIGASSNMNKGGYHIIKNITSGFPGKIYPVNK
                     NYPDMLGLPCYKDLASIPGNIDLAIYFIPSKELPDTVNECAKKGVKGIIIESGGFNEA
                     GAEGARIQKLALDNAARAGIRLWGPNCMGFIDANKRYVFSFINSLVWPDVLRGGNVGM
                     IVQSGMLSAGFLLHALQEGIMGVSKVCSIGNKCDINENDILEHLIDDKDTEVIACYLE
                     SLVDGRKFLDLAKKTEKPIIVLMGGRSQEGAKAAQSHTASLSGNYKIASAAFRQAGII
                     EVFDPAELTDIARAFAKNMPFHPEKEKGTAILTFSGGAGTVTTDLMEDCGLKLAKLSD
                     RTLAAVAELFPPWNKPDHPLDLWIAIERHGFEKVFRNSLTAVINDPGVDSIIFHSYAS
                     PLIGQDFFEELAAMIKKNKKPAVLWIEGRKDFAEHIRGIAENAGLPAYREMNRCVTVM
                     NGMKRHFTKKPMS"
     gene            9677..10207
                     /locus_tag="PLF77_03395"
     CDS             9677..10207
                     /locus_tag="PLF77_03395"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HPC07931.1"
                     /translation="MARIPDFQPGPADTEKKFLQGKPPFDLNIFKIITHAPVGIAREF
                     IGLPASVLMAGKLDPVLREMAITRAGILCNSAYEVHQHRKLSKLVGLPEEKIKALDTG
                     SASPVFSGIEKLVLRFTEETVLQREVSDATFAEISRHFSEDLLVELSIAVCCYMAVSI
                     FLNTFEVNLEENDVPF"
     gene            complement(10681..11652)
                     /locus_tag="PLF77_03400"
     CDS             complement(10681..11652)
                     /locus_tag="PLF77_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC07932.1"
                     /translation="MKICPVCQTRFKDKTQKCRYCNVVLEPLTPASASQPPQPDQQAK
                     QPKIVKDSRKKRQGKAPIERQTGFGKTWNRNKYPFIVVIILIVGILLAIVQKCSEKKV
                     EVSNTQAVEQQAVYAPVNQPAASDNRMIAAAPARDEANGLFRKAFELCISGKCTDPRK
                     AIEYLSEAIKLKPDYADAYNNRGNAYIEIGQYPEAVQDYNESIKLQPQSIYGYNNRGL
                     ALSKLGKHQEAIADYNEAIRLKPDSAIIHNNRGAAYLALGQYQKAVEDFSEAVKIKPD
                     YVDAYYNRANVYFDHMNKEDGCNDAQKACELGKCDLLEKVKGKGYCS"
     gene            complement(11982..12386)
                     /locus_tag="PLF77_03405"
     CDS             complement(11982..12386)
                     /locus_tag="PLF77_03405"
                     /inference="COORDINATES: protein motif:HMM:NF020543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1893 domain-containing protein"
                     /protein_id="HPC07933.1"
                     /translation="MLIPDFSKYSLALFEGGALIHASNGKGVRPLFEALEKFRAKSGL
                     ILHDKIIGLAAAKLVVYSGIIAKVNTTVASAPADRFLKDHGILMNAAGITANILTQDK
                     RAVCPGEVIALKTKDPADFIRQIKTMLDNQRK"
     assembly_gap    12482..12581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12582..13029)
                     /locus_tag="PLF77_03410"
     CDS             complement(<12582..13029)
                     /locus_tag="PLF77_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07934.1"
                     /translation="MMHSSTTASGTTLYRRMAEVFFLLIVFPFVLSLINLEFAQQWKV
                     HFFPAAIILAALIFGSTGGLVAGVAGSLYSAFMLGNPYLIIGNAILGFFTGLFYQKTD
                     KLILSVFLAFICQLPWLVLSDLYFMHLSNAFILKLVVVLFLADILWA"
     gene            13173..14216
                     /locus_tag="PLF77_03415"
     CDS             13173..14216
                     /locus_tag="PLF77_03415"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HPC07935.1"
                     /translation="MKDLFFEPVKGGKKPYLESLRKLLQHLRPPFSAGDTVGIKLHWG
                     EKGNKGFLPPDYAREIAAWLIAHGTKPFVFDTTVLYSGGRRTAEDSLITAASHGYTHD
                     FLGCPVIIADGMDSRNLVDIPAGFNHFRTVQVANVFENAQGFVIFSHFKGHMVSLFGG
                     AIKNMSMGFASRAQKQRMHSDYHPVLNKKKCTRCGVCAEICPTGAATIATSDYPTYDL
                     KKCIGCAQCIAMCPEMALQIFWNSDMTVFQEKLVETAAAVWKIIKDKTIVINSLLNIT
                     VECDCLPGKNPVIHEDMGFIGGYHPVPVDAESLKMTGADIFEKVHPGIPWQRQFTYAR
                     EIGFSPARNITGF"
     gene            complement(14244..15236)
                     /locus_tag="PLF77_03420"
     CDS             complement(14244..15236)
                     /locus_tag="PLF77_03420"
                     /inference="COORDINATES: protein motif:HMM:NF014027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HPC07936.1"
                     /translation="MYDHDTSKFQSVKEFIKHNYRHFNSAVVVDAAEAYANHIHNGGK
                     MLMALAGAMSTAELGITLAAMIRSGKIHAVCCTGANLEEDLFNLVAHKHYKRVPNYRD
                     LKPEDEAELLRNKLNRVTDTCIPEEEAMRKVEGKLFSFWKKAEDENKRYFPYEFIMQI
                     ISSGVLKDQYEIDEKDSWLVAACEKNIPIFVPGWEDSSLGNYFVSSVRRKQLKHYSIV
                     KSGLETMDALIDWYLDASRHSETGFFQIGGGIAGDFSICVVPLIQQDMKMDCRLWSYF
                     CQISDSTTSYGSYSGAVPNEKITWGKIRPDSKKFIIESDATIVAPLIFAYVLAE"
     gene            complement(15419..16486)
                     /locus_tag="PLF77_03425"
     CDS             complement(15419..16486)
                     /locus_tag="PLF77_03425"
                     /inference="COORDINATES: protein motif:HMM:NF025957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPC07937.1"
                     /translation="MIFENVALHNVDGLYKHKDIEGLLLGRIPEHVSARLSKAGQMMM
                     ICPSGSEIRFVSETYPVKITLSVDKITKHLGDGIITDARVFFGTFQTRQRFVIKRIKT
                     LLEIIRPPKFIELAEKIATDAPFSPHVCRIRFWGTTMGAPIRFHGIETEGKIRPPHAE
                     ELPGLSYLAYGTSLTQGAYASESHLSYPNLVGCRLGVDVINLGSSCSAYCEPEMADYI
                     AGRKDWSFATLDLSLNMVDTFSPEEFSRRVSYMINRIAGSNKSRPVFCITLKPYLGDY
                     IESKKPDLYRKLLRKAVENSALDNLYLIEGQDLMPDVAGGLCPDMVHLGDFGTIQIAE
                     KLAAKIKPVLQSHHLLFKHSR"
     gene            complement(16520..16837)
                     /locus_tag="PLF77_03430"
     CDS             complement(16520..16837)
                     /locus_tag="PLF77_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07938.1"
                     /translation="MIIRKSAVVAVMLIALFLLSCGMSDPFSGKWFNDVIEKEMLTIT
                     KSGSAYKVDTALGSFPCIKEGDLLKCQMGIGDTMLQINENRCLVMKTPAGVEFKFRRQ
                     ENK"
     gene            complement(17097..18314)
                     /locus_tag="PLF77_03435"
     CDS             complement(17097..18314)
                     /locus_tag="PLF77_03435"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPC07939.1"
                     /translation="MTYYIIIAFCVVILLAYFFDVTSRQTKIPGVLLLILTGMAINSI
                     DSYFNFEVSQIDVILPVMGTVGLILIVLEASLDLTLSKQKKSLILKSMSSAIVLFLVF
                     TGLFALMLSYGLGYDVRTSLVNIIPVAVISSAVAIPSAVGLSGNNREFVIYESSISDI
                     FGILAFNYVVISNGHLFSGILNFLLEIIVTLFASVLFSAFLAVILHKINHHVKYIIIM
                     TAIILVYALSRLLHLPSLLVVLIFGLVMNNNNLFENSITAQFINFQDFNTELNAFKNI
                     TSEMTFIVRSFFFVLFGYYTSLFDLLNLNNLLFSVLIVAAIYALRALYLKSIIRISMT
                     PLLYFAPRGLITILLFLSIPQAMALPYMNTGVVTQIIFITILIMAYGNIVFGKTGEAI
                     PTGTPQDKKNLSG"
     gene            complement(18402..19106)
                     /gene="radC"
                     /locus_tag="PLF77_03440"
     CDS             complement(18402..19106)
                     /gene="radC"
                     /locus_tag="PLF77_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPC07940.1"
                     /translation="MIQSESKHSKGINSWPEDDRPREKLLRQGAETLSNSELLAILLR
                     TGVKGASAIDLARRIVEKFGTFRNMIHTDVREWKEFKGLGPAKMAQIQAALEIGRRFR
                     EVEALKGKQKIASCCDVVEIVMPQMRDRKTELFKVLYLNSNNKIIDISDVAEGTVNHA
                     IPIVREIIHAALQKFAAAIICVHNHPSANISPSAEDRKFTQDLLAAGKVMGIKVLDHI
                     IIGDGNYYSFAEEGIL"
     gene            complement(19133..19966)
                     /locus_tag="PLF77_03445"
     CDS             complement(19133..19966)
                     /locus_tag="PLF77_03445"
                     /inference="COORDINATES: protein motif:HMM:NF005881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC07941.1"
                     /translation="MKMLSNKNVLITGAASGIGRATAAAMGLAGCRLFLTDINAEGLK
                     KTADMISSAGDNVCLSRPFDVGDYEAMRAFASEIHAEFGSLDILVNVAGVALFAKIED
                     MQHRDWEKIIRINLWGVIHGLECFVPEMIRAGKGGHVVSVSSTAGLIGLPWHVAYAAT
                     KHALVGMSEVLRYDLRKYKIGVSVVCPGAVHTGLVNTADIRTDDHEATNKARKMFVKI
                     AMTPEKVADLIIQAILKNKYLVITSLDVKLFYFIKRTCFPLYHLVMLIINRFMDKALK
                     K"
     gene            complement(20031..22436)
                     /locus_tag="PLF77_03450"
     CDS             complement(20031..22436)
                     /locus_tag="PLF77_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07942.1"
                     /translation="MMKFVQNFRHDWQQNIIKRPPPRRIVSILKKAWQLEEYYADIFE
                     KNNYAVRGNAGISTADAEYSAILPSFQLDVTEDANRAEESSDSRKFRFKYNLPFKKID
                     PRVVSSALATSPKVNKKILINLINHLHFTKEQVSIHIRDLNTGDEILQQGEPGPYLND
                     TVTIRFIDPGAFDLERHHPLNLIIDNRKSLIVFPIDLQSVNHESIVFKMPDVAHSYSK
                     RQAVRHTCEGVDAEIVQGTLNVHGVLEDINPHDLRVCIRAPEDINPANHLFLKLSQSD
                     QTHFMGECQMLRSDNEGSYIILQPVHQNRPVLSPRKYAADRVRITPPPVINFIHPLCG
                     LAVQNKVDDISASGFSVTVSDNESLLIPGMLLPQMTLHLPGMFNDLVCAAQVVYRLKL
                     KKNMVKYGFYILDMSLNDQKRLFDAVSKAADPHVNLTGRVNMDSLWELFFDSGFIYPD
                     KYGIISPYTEALKETYRRLYEEGQDIFTHLTYQDQGQVYGHLSMVKAYENSWIIHHLA
                     ARPLKGKRTGLKTLNYFINYLDCLFRLPVSSKTMRYTFCYYRPENTFSDYYFGGVCKI
                     FKRREICSMDLFGYMPMTVDAEKTGLPSDWSLENFSRDDLALMREYYSGIGGGMMLDA
                     YALECRAAETELSGQVHEVLTENKNNIMDMYNKIGLKRESRVLSVKHDGRIKAALIVD
                     TSDLGVNMSELLNSIKIIVIDHTLPWPVLQDAVSIAGKIYNSDSIMVLIFPYAYLGQQ
                     GVECKKRYNLWVLNSNLDSPEMDIIKDRAKITKRKFITEKFIKEIARNRLKRLLALIQ
                     KQQ"
     gene            complement(22528..23340)
                     /locus_tag="PLF77_03455"
     CDS             complement(22528..23340)
                     /locus_tag="PLF77_03455"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC07943.1"
                     /translation="MTQTQTYLMENSDEEKRLDIKTDPDAVKEQALWFGMGPGARVLD
                     VGCGPGKTTALLHDVVQPDGEAVGVDIAESRILYARSFYGYRKGIKFDVRDVRQPMAD
                     LGRFDFIWVRFVLEYYREGAFDIIKNISANLKPGGYMCLLDLDHNCLCHWEMPREMED
                     VVIKAMLHMQNCFNFDLYAGRKCYAHLYDLGFKDLTLNVVAHHLIYGKLQHSDEFNWM
                     KKLEIGALKAPEIFESYAGGSKKFLDDFRIFFRDPRRFTYSPMILCRGRKPL"
     gene            complement(23416..25077)
                     /locus_tag="PLF77_03460"
     CDS             complement(23416..25077)
                     /locus_tag="PLF77_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPC07944.1"
                     /translation="MRQYVLGMANPGTVYKMIGKVTSQFSRSSIYESREINPQKIEIT
                     VTPKEGVQEQPFQCENRMGHFESISLVFNNAIPEIEHPECMFKGGDCCRYIISWGNNR
                     SYLLKRTRNIIAIVMAIVAIILTIIYPVDELVYFIPIIIITILIINFLNDKQTKKELR
                     DSILNLEHSRDDLIQQMEINYNNSQMVHEIGQAINKYININEILDNVIQVLEKRLGYD
                     RCVILFSNEDATRLVFKAGYGYTEKQMAIVKSTEFDLTKPQSKGVFVTSFREQKPYLI
                     NDISTITGKLSQKSLQFVKDMGAQSFICCPILSDTKSVGILTVDNFKTQKTLVESDLR
                     LLMGIASVLGVSIRNAELHEQRNRQLKSILKALAASIDARDPYTAGHSEKVTEYAVGI
                     CKEMNMPSDYTEVVAVAASLHDYGKIGISDALLKKEGLLTAEEYETVKTHSVKTRKIL
                     EKIEFHGPYSLVPEIAEAHHEKLDGSGYPRGLKGHEIPIGSRIIAVADFFEAITAKRL
                     YRDPIPVDEAFEMLRRESDVHFSREIVEAFIKFYTSNNMASQKSM"
     gene            complement(25507..26277)
                     /locus_tag="PLF77_03465"
     CDS             complement(25507..26277)
                     /locus_tag="PLF77_03465"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPC07945.1"
                     /translation="MTAQYLLLDVEENVATATINRPDKGNSLSPQVLEDIEHTFTELS
                     RRQDVHVVVLTGGERYFSAGFDLTYIRTLQKAHNEAYTALFHRAYRAVMFCEQPVICA
                     VGGPAIAGGFDLTMMCDIRYASERARFGQREIVLSLNPTIDPLWRIVGLSNAMESALT
                     GRIWDAQEALRMGYISRIFPEGQLLKEVGAIAREMATYDRGCLKETKQLAHLLLNSSL
                     DQAMRQQEWLFRTYIGSEDNHQRIDDLQTKLASMRRNK"
     gene            complement(26306..28729)
                     /gene="lon"
                     /locus_tag="PLF77_03470"
     CDS             complement(26306..28729)
                     /gene="lon"
                     /locus_tag="PLF77_03470"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPC07946.1"
                     /translation="MTEQTVPQQNKNTFNIPEIIPILPLHNVLVFPKTMIPLEVTGSA
                     SVLVDEAMTRDRLIGLIMSRKEPDKPNDYKKDDLHPVGTCAVIMKMAKTMDNHTQLLL
                     QGVSRFSIEEIIEGKPYQQARIKLLEEKDAKDLETEALMANLLSLFDRILKLSPFLPP
                     EFGPMAKSITEAGTLADLIASIINAPVEEKQKILNIADIKKRLKELTLMVNHQMDVLE
                     LGNKIQTKVKDDIDKSQREYYLRQQLKAIRQELGETDENAVETEEYRKKIEEKNLPEE
                     AKKEALRELERLSRMHPSSAEYTVSSTYLDWMTTLPWNESTNDNLDIGKARRVLDEDH
                     YGLTKPKKRIIEYLAVRKLKPDSKGPILCFVGPPGTGKTSLGQSIARALERKFVRISL
                     GGVRDEAEIRGHRRTYIGALPGRIIQGLRRAESNNPVFMLDEIDKVGSDFRGDPSSAL
                     LEVLDPQQNNTFSDHYLDVPFDLSGVMFIATANMLDTIPPALLDRLEVIELNGYTQEE
                     KVKIAQRYLIPRQLKENGLNSSQFKLTHKALNAIITGYTREAGVRNLEREIANACRGV
                     AARIAEGAVKSGTLEEKDIPAYLGPVRVMTDLDARIAKPGIAIGLAWTPVGGDMLFIE
                     ATAMKGKKGLTLTGQLGDVMKESAAAALSFIRTNAYALNIKPEFFDDTDIHIHVPAGA
                     IPKDGPSAGVTMFAALTSLLTNRKVKKQLAMTGEITLRGAVLPVGGIKEKVLAAYRAG
                     IKTILLPEWNRKDLEDIPGNVRKSISFHFVKDMMDIIKLALEPVPQKKMKRASARKSK
                     PAVPSKTKK"
     gene            complement(28769..29209)
                     /locus_tag="PLF77_03475"
     CDS             complement(28769..29209)
                     /locus_tag="PLF77_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPC07947.1"
                     /translation="MTYIKINFGSNFEDEFQKVVDEVFHLVRPAFHNYECIWRPNVDV
                     YESTDEIIVLADMAGLNKEELHIEVNRKQIKIAGVRKAVQHLQNAHYCQAEIPHGYFE
                     RIISLPAPVESQSAKASYADGILMVRINKLPVSKSHRISIISEK"
     gene            complement(29449..30456)
                     /locus_tag="PLF77_03480"
     CDS             complement(29449..30456)
                     /locus_tag="PLF77_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPC07948.1"
                     /translation="MTKKIIMLVLLMAVLFHIAWYAVSRSVQHEKIPDDATAQAAFDK
                     LLKDEEISIDRHAFTEMSIEANGIRIHLDVFSKGSNGPTIVFIPGTSVYSKFYIESMY
                     KLYLQGFNVVGFDPRGHGMSSGLRGDYTVNEIVEDTLAVVKFARKRFSGPVAVVGSSQ
                     GGIAAFYAAARDDSLAAAVCHNLADLNGKDNLILSQIRPPVWMAPIAGFLADLYKRFS
                     VPVFLYLDLTQEKSRSGTDAKTLIKNNPVTVKWITLRALGSLMKTDLAKPVEQIKVPV
                     MLIHAERDSIFPQAYVEDIFNRLTCPKKYLFFKDTEHLVMTNNVDDVVPPIAAWLRDM
                     M"
     gene            complement(30453..31304)
                     /locus_tag="PLF77_03485"
     CDS             complement(30453..31304)
                     /locus_tag="PLF77_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPC07949.1"
                     /translation="MRIEKIEDKCSECMLCVHDCVMGVWRVVDGQSRPVAVDLCNGCS
                     HCIAICPCDAIVHDGLRKEELAGINRKNLNPDVYRDIVMSRRSIRQFKNEPVPRELIE
                     KVLDLVRYAPTARNHQDVGYIVVTDKPLIEKTAADLFGWGNRLYNMTTSGIGRRIINL
                     TGLNKNPYYNLMAYVQMQNAEKGRDYILHHAPVVILIHAAKRKMFVCDDSNIAAATII
                     NYAHTLGLGTCFVGMMTLMLRFSGRLRKTFGVPDNRRVYATLVMGYPAYRYKNTVSRK
                     KAEVQWR"
     gene            complement(31504..31713)
                     /locus_tag="PLF77_03490"
     CDS             complement(31504..31713)
                     /locus_tag="PLF77_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_177722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HPC07950.1"
                     /translation="MSSIYKVIEVIGGSEKSWEEAAKKAVEMTAKSLKEIRIAEVKEL
                     DMRVEDGKVVEYRVKLRVSFKYLPD"
     gene            32116..33924
                     /locus_tag="PLF77_03495"
     CDS             32116..33924
                     /locus_tag="PLF77_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPC07951.1"
                     /translation="MHKFTLDKPDNLVDWWEESVTKFSERPLFGTKNKEGVYKWATYK
                     EIGNRIDNLRAGLTQLGIGKDDVVGIIANNRPEWAVIGYATWGCLARYVPMYEAELVQ
                     VWKYIINDSGAKVLFVSNPAIYEKIKDFQKDIPTLKHIFIIDSDGENSLTALEKKGAE
                     KPVAPKSPKAEDVAELIYTSGTTGNPKGVLLMHMNFTSNSHAGLKMYPELYENEVVSL
                     TILPWAHVFGQTAELFAITRLGGRMGLIESTKTIINDIVQIKPTFIIAVPTVFNRIYD
                     GLWNKMNKDGGLARALFVMGVEAAKKKRILAEKGQSDLMTNFKVAVADKIVFKKIRER
                     MGGRMLGSMTGSAAMNVEISKFFFDIGIPIYDCYGLTETSPGITMNGSQAYRIGSVGR
                     PIDKVKVVIDSSVVEEGATDGEIIAYGPNVMKGYHNRPEDTKAALTPDGGFRTGDRGR
                     LDKDGYLFITGRIKEQYKLENGKFCFPVSLEENICLASFVQQAVVYGLNRPYNVCIVV
                     PDFDVLLDYAKEKGLPTDINTLVEREDIIHMISEAVTGQLKGKFGGYEIPKKFIILPE
                     AFSLDNGMLTQTMKLKRKVILDKLNDRIEALYKEDK"
     gene            complement(34015..34833)
                     /locus_tag="PLF77_03500"
     CDS             complement(34015..34833)
                     /locus_tag="PLF77_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC07952.1"
                     /translation="MFKISEQESILLFYMGRSPLGILLYPVYAFLVGDVLIDTGTNRA
                     GHEFLEALKGRRIAGIINTHHHEDHIGNNADVQQHFGINIYAHPLTLPYLENPRLNDL
                     HLYQRIVWDWPRPSKGEAVGASVKAGPYHLEVIHSPGHAEDHICLYEPDKKWLFTGDL
                     FCGTNFVYLRQDENYLQILDTLKKLSALNIETIFCNLKGVVQDGKIALAKKISTMERL
                     RDDVFRLRDRGLSPQRIRQKVLGREDARHFFTRGHYSKQNTVDSILSGKRPANR"
     gene            complement(34886..37591)
                     /locus_tag="PLF77_03505"
     CDS             complement(34886..37591)
                     /locus_tag="PLF77_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HPC07953.1"
                     /translation="MFKRTCVPNNFTDFSTRYLSRRGSCEKTFFLVAVVFFLITFFAA
                     PAYTAEETARSARQVPAESGKSSAFTPKETTPIVVSVIINTQSRGDIFAELDDRGNLY
                     IRPDDAKALKLQYSEDRIVILRGDEPHVPLNALLDVTSTFDEKKLTVTVIGKTTESTR
                     TAADLFSLRAETKNIYYPRETSAFVNYGVNYAFTGQGGFQTLSVTNKLGFRTGDMFFT
                     SDSVFTKTESDYDLVRLQSSATYERRKDLQWLVMGDQYANSGELGSTVNMGGIGFSKV
                     YRLDPYFITQPVMDLQGTVIYPTQAEIYLNGVLIGKQDIAPGSFDLKNIYSQTGSHNI
                     EVVLKDPFGNVQRISYMAYFSAQMLRKGLHEYSYNAGFLREQYGSESNEYGDLAFSAF
                     HRYGVTNKVNIGASAEGSDGVYNGGISTTFAVPLAGQFTVQLSGSSSDEIDKRGDAVS
                     LQHNLQLGNFSTNLLLRKYSRYYATIGNPLPTEDNTRHEIRAGMGFSLNPLGSFSVNY
                     AQTENFKGMDTQVISLNYSRTLYRSISLFATGSATRATGEDTNYSGFIGLNFALMDNV
                     RGSAQVNFGSDDVNSETLQVQKDIPVGEGLGYRASVNRSETAANTVYTTNPYVQYNAR
                     YGIYSFDAQIQDSKNGISTESYNLSAAGSMVWAGEYLGLSRPVSDSFGVVLFNKNVPG
                     ASVLNNGQEIGKVGSLGTMVVPTMSSYGQNKITLDTKNIPIDFTISDVNKTLSPSLWS
                     GSCIYFDAQQLRAVTGTIFARKEGKKIPLEYVEIAMKIGEKELLSPTGKGGEFYLENS
                     FGEDDAAGSSTVADKQSCHAITRMIKAGGSTISPGTYKASVEYEGGECGFAVTFPETE
                     EVITELGEIECVATSNSPRRKDTSLRRRSLYYADYAGKALSAIPE"
     gene            complement(37563..38333)
                     /locus_tag="PLF77_03510"
     CDS             complement(37563..38333)
                     /locus_tag="PLF77_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HPC07954.1"
                     /translation="MNKIVGFLAVILFVIIYSTHALAADFQVQPTTMDLGGKVKSGVF
                     SVINNSNEKIDFQVSVKAWNQDENSKDAYTDTNDIVFFPKVMSIDPNSQRAVRIGLKT
                     PPGAREKTYRLFVQEIPTQKKTQDIDVNQKVKAGVTIAFRFSMPIFVKPLKPQEIYAI
                     DKVEMAAGKAKAVVKNTGNVHVKLRSVTFSGKAADGKELYSKEVAGWYILNGMSSSYE
                     AEIPKDVCGQLAKIDINVRTEDKDISGILNVQKNMCPE"
     gene            complement(38487..39593)
                     /locus_tag="PLF77_03515"
     CDS             complement(38487..39593)
                     /locus_tag="PLF77_03515"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HPC07955.1"
                     /translation="MRFENLKIACVIPVLFCLLTLSATVHAAINCPGTLPEGTRGVAY
                     SYTFTATNGNGGSPWWRIVMGSLPPGLSLINQSLNSVTLAGTPTTAGTYWFAINHRQP
                     GMDPICFSTIVINAAGCVLNGTDTGGISFGNLDPSGYPAPVNNIITQQVNFQCSAGLA
                     YSIAVTNPVGQLQLTGANTVPFTLGLHANGMGAGAVDIPLLTTNSAITSYAGFVEGVS
                     SFSNPITVEINWAGGSLSAYVNVSDVTVIPACGTPTNGTMNFDIDPSLNTTITASTTA
                     NGTSPTVKCTNASQPNVTCETSGGSTAGQLKKGTDNINYNVTTCGVSGKITGNGFGAA
                     PASEIPVGISVVPSAYQSAPPGDYSDNVTVTIAY"
     gene            complement(39697..40230)
                     /locus_tag="PLF77_03520"
     CDS             complement(39697..40230)
                     /locus_tag="PLF77_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07956.1"
                     /translation="MKKIILLMAALAAMSFAINPVFAASPAGFTSAEIKVNAQVGVSC
                     REIQKGSFPSPLLIDTQTAAEQTFPNSADELVRCTSGTVFTVKISSANGTALDETCTS
                     GGVGDMALQSSSWPSDVISYTFTCGGDTDGNGRFTGAGYSTNKALGIGIKIAAADAQA
                     AVAHNDYSDTVTLTITY"
     gene            complement(40257..>40564)
                     /locus_tag="PLF77_03525"
     CDS             complement(40257..>40564)
                     /locus_tag="PLF77_03525"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HPC07957.1"
                     /translation="TSTGSATTVQCSKSKAFTVTAASAGAGGAEDNDGSLAGVLTGPG
                     ATIPYTLAYTSSFNGAGFGSATPTTLLNANAATVAIVDANAAEEGTYSDTVTITVEY"
BASE COUNT        10534 a         9926 c         9259 g        10745 t
ORIGIN      
        1 aaaaaacatc attcgctttc aggaatcttc cggtttgata atcgatctga tatattccgg
       61 caggcgaatt atcaaaaagt tgtctgtatt tttcttcgtt ctcgcgcaac ctttcttcca
      121 tcattttacg ctcggtaatg tcaaccacga ctgtaataat gtagcgttca tcatcaatgg
      181 tgatcagttc tgccgattgg agacattgaa gcattttgcc gcctttgacc tgatgacgca
      241 cttccatatt cctgtatctt ccgtttctca gaagacgttt ttttatttct tcacgatcct
      301 gatcgagcca tatgcccagt gttgaagccc tcttgccgat gacctgtttt cttgcaaacc
      361 ctgatttctc gcagaaagaa tcactcacat catagatggt gccatcctcc aatttcgaaa
      421 caacgatggt attgggagac atgcgaaata tcgtgcgata gaccatgccg gttgacatca
      481 gcgtctgccc gacttttgtc gtgttcagga gattttccag ctctttaatt ttatttttca
      541 gctccttaat ttcttttaac gcctgattcc tgccggtttc cttcatgaaa aagcacctcg
      601 tccggtttga tataatttgt tttttatata aacaagcctg ccgattgtca acgtacacga
      661 tcaatatccg tcattgcgaa tccctcataa ctaatcctga cgacatttca tttacgcacg
      721 gttgcgtcat tttcaggagg caaatatatt ctcgcttaaa aaatgaacgg gatgaagcgt
      781 ttggttcgtg tcatatatac gcgatacggt ccgccaatcg tatcgatcag agctttttcc
      841 tctgctttta ctctaaggct gtagacaaaa caattctcca ggaaaagaac ggcaaaactc
      901 aaccagttgc ccagggccat accgatcccc gcaaacatga taaaagctcc gctgtatgcc
      961 ggatggcgaa tccagcggta agggccttct tcaataaccg gctgatccgc ctttacaaca
     1021 acctccggag taaagtattt tcccagagca cgaatggaat attgacgaaa tatactgcct
     1081 gcaatgagca tgcccgttcc aacggccagt gccggacgct gccatgtgat gacaaaccag
     1141 ggtgtcagcg atgccattaa cgccagaaca aaagcaacgc gataaccgat tttgattaaa
     1201 agacgggtcc cggcatcctg cgtgttggaa ggatttctgg caagggcgcc ggcaaattta
     1261 atttccagat aaaacgatcc gattaacgcc gtccaaaaaa gaacagcttc cggatataca
     1321 aaaaccggcg gtatatgcat gtcgcatgtc tccaaatgac atatgtttga aatgtcgttt
     1381 catgttcaat ctccaacgtt cagaagaagg gaaacgggac atcagtgttt tttcgggata
     1441 tcctttttca tctcatgcat ggcctgcatc aactcatcga tgtccacttc cgggttttcg
     1501 ccgtatccct gcataagcga ttcgtcatac atctgttcca gttgcctcag ttgcaccaga
     1561 gcctccagat tctgcctgaa atcattttcc atcattttgt taacggcgtc tccggatttt
     1621 ttcagatcac ttaaatcgat gttcagggat gtgcgggaaa gaactttttt caaatactcg
     1681 cagcctattt ttgcattaaa cgtttcaata tacggcggga ctctggcgac aatgctggcc
     1741 gcctctatcc cttccctgcc ggcatgattc accagcagcg tcgaaaagct gcatggcccc
     1801 gtgtaattca ggagcgggaa tgattgaaac tcatgcttca tctccggacg gctcaacacg
     1861 gcataaacga ttgtttcccg ggtgtgggga acctggtcaa agacgccgcc cagaacatag
     1921 atccgacacg cctgataacg tttggcgatg ttcaaaacag ccgatgtgaa ttccggccat
     1981 gcgacgttcg gttcaaaacc ggaaaaaaga atcaggtcgt tctctttacc gctcttccag
     2041 aaataaaatt cattggcggg ctcgttgagc gatttgatca ggcctctttc aacgaccgca
     2101 tgcggacgca tgacctgcgc cggatcgaat cccggtacct gccagatata gaaattccgg
     2161 atatcaatgg aagcgaactt tcgggcatcg agtttgcggc gcagatagtc gatgcttccg
     2221 gtcgccatct caccggcatt gagccagccg cggtaaccga ttagaatata gggattgata
     2281 aggtcgggcg tttcctgaaa aatgagattg tcgttcataa gccctcctga gatggaactt
     2341 gcagttgcgg ttttaaaaac ccatactaca tttaattatg gtttgtccgt tcctttcagt
     2401 caagtaatta atgaaaccct ggtttcagaa gagtagcaca caaaaactcg tagttgaatt
     2461 atcagttatg gtaatttgag ttgctggtgg tagtgggggc aaagaaccca aaatggtctt
     2521 tctccccgaa gggcgttatt acggcagctt gtaccggtcg ttcctatgcc gggaaatcaa
     2581 caacctgctg gttaccggca gagcgatttc gatggatcgc ggcgcgctgg cctcggcgcg
     2641 tgtgatgggc ccgtgccttg cggaaggaca ggcggcggga acggcatgcg cgctggctct
     2701 ttcgtcggga caatcgattg atgccgttga caccggcaag cttcgggaaa tgttggccaa
     2761 ggatggcgca cggctgaaat gaacttaaaa aaatcgttga atgttcttcg ctcaacgttc
     2821 cttgtttaag aattaaaaaa aatgacaatt ttaagtggaa cgtggtaaaa tataaaaaag
     2881 ttcgatacag atgttcctga ttccctctcc tgctcagaaa acttgatccc gctcttttga
     2941 ctggtctgtt tgcggaagca ttgaaccccg tcaacacaat tcaaaggagg tcacttatgc
     3001 cggccaaaaa attgaaagag tttctggaca gccatggagt gaaatatgtg aagctcacgc
     3061 attcgacggc gtacacagcc cacgaaattg ctcaatccct gcacatcagc ggaaggaagg
     3121 tggcaaagac cgtcatcgtg atgattgacg gaaagatggc aatggcggtc ctgccgggca
     3181 atcgctccgt caatttcgat cattttcaaa aagccgtcgg cgcgaagaag atcgaacttg
     3241 ccaccgaagc gcaattcaaa ggcatgttcc ctgattgcga gctcggcgcc atgccgcctt
     3301 tcggcaattt gtacgggatg gaggtttacg cggatcagca tctgacggac gatgatgaaa
     3361 tctttttcaa cgcctgttcg cactcggaac tggtcgagat gtcttatccg gattatgaaa
     3421 gactggtgaa acccaacgtg gtgaagctta cgtgatagcg ttctaagttc aacgttcaat
     3481 aaaacatgaa tgggaaatat caagaaattc aggttgaatg ctacagcggt tttaaggcga
     3541 atgaacgccc ggttgctttt acatatcagg gagagcggca ggaaattcag gaggttgttg
     3601 atcgctggta tcacggaggt ctgtacagca gcaaacccgt tattaatttc ttcaaagtaa
     3661 ggaccgcaga cggcaaggtc tatcttctgc gctatcagtt tgattcggat gcatggtcgc
     3721 tgcggatatg atgaccgcag acgtcaggat gatgatttca gaatcttcgt tttcagtccg
     3781 gtgtgaaaag attgatctta tccttggaaa cgactctgcc cgttacaccg cagaagtctt
     3841 tctcaatcat ggctgaaaac agagacggat ctgaaggtgt aaaagcggca aagcgtttcc
     3901 cgttttcttc ttttccgatg acaataccct tgttcgccaa attgtcccgg tcgtaaatca
     3961 cggtataggt gtccaccgtt gcccgccccg aaggcgtcgg atcaatctca tatttaggcc
     4021 gtttatcgac tgcctgctgg cacgttcgtg tatcggctgt cgccgccgca ttgttctcgg
     4081 gagaccgggt agagcacacg gctgttgagt gcttggtcat gtaaaaagaa tttcccgtga
     4141 caagaccggt cctgccggga tgccggcgca gaacctcaac catttgcgcc atggagtgca
     4201 tgacatagtt gtttcccggt ccgccgaaat agggaagtcc ccctgtaacg gtcagatcgc
     4261 ggctgtcatt ttcgggaata cccagcatgt ctcgggttac ctgcacggca accggaaaac
     4321 aactgtacag atcaaagaaa tcgatatctt cgatggttaa accggcctgc ttcagggccg
     4381 tccggcctgc gatttcaatg gcgggagaaa aaccatagcc ttctctttcc aagacattcc
     4441 agtgatcgtt ggcttcggca taaccgtgga catacaccca ctttaacgga ttcacgccca
     4501 tcttttgtgc ctgtgcttcc gatgcgacaa ggagtgcggc ggcctgattt accaacagca
     4561 tggcattcat ccgtttggta tacgggaaag cggtgttgcg attcgtttcc gaaagggtca
     4621 tgatttcttc cggcgtgaac gaatgctgaa accaggcaag aggatttttg gcagccacca
     4681 aggagaggcg gctcatcagc ctgcctatct tttctgcatg ctcttcaatt gtgcagccat
     4741 agcgccggcg aagagcattc tcaaacatgg gatagacata agcgggaagt gaaagtccgt
     4801 gaatgtgctc tgtttcgcag gcgccgtcaa acagccccag cggagtcagt ggcggcaggc
     4861 agtgccgacc ggacgccacg ttccagccgg taaaggtttt ggaatttttc gcctgctgca
     4921 tggtgtgttg agcctctgct ccggtcaaaa taaccagacc ggctttccct ccggcaatct
     4981 cctgcatggc atgatgcagc acggcgtgcg gcatgacacc gccaaagccg gaaactctgc
     5041 atcgtgcgtc ggtcagattc agtttatcgg ccacgcatcc cggcgggttg gcaataccgt
     5101 catcggaaaa aagcgacgtg gtcatcagca ggtcggcctg ttgtaagtat ttttctgtca
     5161 cgccgccatc ttctgccgca attttcacgg ccgctgccat aaggtctatc ggcgaaccgc
     5221 cttgcacaga tgatgcctct ctttttgtca cctgccctgc cccgataatc accggaatat
     5281 tttcagaatt catttttcaa cttccgttta tttctttatt tttaaagatg ttttactctt
     5341 gttgcctctg ccgaacaaca cacgcagttc gtctttggcc tgttccaata ttgcttttcg
     5401 ttcggccggc agttttgtgc ccgcgcgatt gatataatag ttcagcatgg acatggcgga
     5461 ttgaaaaggt tccgcttttc tttgcgtaga gctctcggcg gattctttaa gcgattcggc
     5521 aatccgtcgc ggattgtcaa aagtaaatac accggattgc aaatcgaggg caaagctggt
     5581 ttccttgact ttttgcgtcc agctttcttt tgttgctttc ttcattgcaa aattatacca
     5641 gattcctgcc gtgcattcca tattcgcggc tgatgcaggc taacgtcaga attacttccg
     5701 gtttcccatt ttgacgacgg cgtcgaactc gtcggtgatt tctctggcag gcgctttgct
     5761 gagcaggctg acgacgatga ttgccaaaca ggcgaaaaca aaagcgggca gcagctcata
     5821 aatgttccaa acgccgccga ggggccgtac gagatatttc catacaaaga ccatcacgcc
     5881 tccggaaacc atgccggcta tggcgccgta tacattggaa cgtctccaga aaagggcaaa
     5941 cagcataacc ggaccgaagg ccgcgccgaa accggcccac gcaaacgaaa taatgacaaa
     6001 aatcgagctg tccggatctc tggcgatgaa cgcggcaatg gcagcaatca caataacggc
     6061 cgatctggca atcatcatgc cggttttttc ggaaagccta atgccgaaac attcctttat
     6121 gagattctca gtcacgctgg aggaggccac cagaagctgt gagtcggagg tggacatggt
     6181 ggcggccaga atgccggaaa gaacaacacc ggcaattaaa gagctgatca cgccggtgtg
     6241 gctcagaagc tgtgccatcc ggatgatgat tgtttcggat gcgcttcctt ccagtgcggc
     6301 aatctcaccg ttgagggaca gggtttgacc gacaatgccg atcaaaacgg ccacggcaag
     6361 tgaaaccaca acccaggtca ccgcgatctg ccgtgaaaaa acaattttct tttcatcgcg
     6421 gatggccatg aatcgcaaaa gaatatgggg catgccgaag taacccagtc cccaggccat
     6481 tgtagaaatg atggtaatca gtccgtagcg ttccgtctga ccggtcgtgg aattatgtga
     6541 aaacatcata ctgagataac cgggaatagc atccgccttt tgccacacgg catcaatgcc
     6601 gcctgcaaca gcagcgccga acatcaaaat gctcaacagc gcaatgctca tgacaatgcc
     6661 ctggatcaga tcagtagtgc ttgcggcaag aaatccgcca accgtcgtat aggcaacgac
     6721 aacaaccgca ccgatgagca tggccgttgt gtaatcaaga ccgaacacac tattgaaaag
     6781 ctttccgcag gaggcaaaac cggcagcggt atacggaata aagaaaatca agatgacaat
     6841 gctggcgatc catgtgagaa tttttttgga atcgcgataa cggttggaga aaaattccgg
     6901 cagggtgatg gcgttttgcg acagttgaga atagcagcgc agacgacggg ctaccgtaag
     6961 ccagttgaca taagtgccga tcgccagacc gattgccgtc caacccgcat ccgcaatacc
     7021 ggacagatat gcaacgccgg gaagtcccat gagaagccag ctgctcatat ctgatgcttc
     7081 ggcgctcatg gcagtgacga tggtcccaag ttttctgccg cccagataaa aatcgccgat
     7141 ctgatcattg cgtctggcaa agacaaagcc gatgacgatg accaccacca tgtaggcggc
     7201 aatggacatc agcatataaa aattgaagtt tcccgtcata atctctcaac catcccttca
     7261 accattagtc gggaatgaaa ccattaacat gccgcttatt attttttctg acgccataac
     7321 attgctgcaa tagttatgct ccccggaacc ttaaaaagtt ttgttacaat tttgtcgatc
     7381 attcgtcaac cgccagttgc gctcaataaa ggttcttgac aattccggat aaaataaact
     7441 aatgatttaa cgttaaatga taaggagata ctatgttgga tgtaatagaa aaagctttga
     7501 aggaaggacg aaagactctt tcggaatacg aatccaggca ggtaattgaa tcagccggtg
     7561 ttgcggtggc ggtagcagcg ctgaccaaaa ccaaagaaga agcgatccgg gaagccgaac
     7621 aaatggggta tcccgtggcg atgaaagggt gttccgccga actttcccat aaaacggaag
     7681 cggggatggt tgcactgaat gtcaacagcg cgcaggacgt cgcacaggtt ttcgacgcac
     7741 tgaccggcaa ggtaaaaaat ctcgacggcg tgctggtgga aaagatggtc agggggtcgc
     7801 gcgaactggt gatcggtctt tcgcaggatt cgagtttcgg cccctgtgtg atgttcggcc
     7861 tcggcggtat ttttaccgaa gcgctcaagg atgtgacgtt tcgggttgcc ccgctgacga
     7921 aacaggatgc gctggaaatg atcgatgaaa tcaaaactaa aaaattactc ggcgaattcc
     7981 ggggcagccc ggccgtggac cgaaatgctc tggccaaagc gcttgtgggc attggggatc
     8041 tggccatcaa gtatgaagcc atcgccgaga tcgatatcaa tccgctgatt gtctgcggag
     8101 acaaaccggt tgccgtcgat gcgctggtgg tattgaagta acgctgtcat tacagaacac
     8161 tttttgaaca tcgtgacaat ccaaaaatat tttggtccct gttctcaaca tgacaattca
     8221 ttgccctgca cgtgtgccag caatgcataa aacgaagatt tgggagaatg taatgttaga
     8281 ttattttttt aatccgaaaa gcgtcgccat tatcggagca tccagcaata tgaataaggg
     8341 cggctatcat atcattaaaa atattacgtc cggatttccc gggaaaatct atccggtcaa
     8401 caaaaattac ccggacatgc tgggacttcc ctgctataag gaccttgctt caattccggg
     8461 caatatcgac ctggctattt attttattcc ttcgaaagaa ttgcccgaca cggtcaatga
     8521 atgcgcaaaa aaaggcgtca agggcatcat catcgaatca ggcggattca atgaagccgg
     8581 agcagaaggc gccagaattc agaaacttgc cctggataat gccgcccgag cgggcatccg
     8641 gttgtggggc cctaactgca tgggctttat tgatgccaac aaaaggtatg ttttttcttt
     8701 tatcaattct ctggtctggc cggacgtttt gcgcggcggc aacgtcggca tgattgtgca
     8761 aagcggaatg ctttccgccg gtttcctgct gcatgccctg caggaaggca tcatgggcgt
     8821 cagcaaggtg tgttccatcg gcaataagtg cgacatcaac gaaaacgaca tcctggaaca
     8881 tctcattgac gacaaggata ctgaggtcat tgcctgctat ctcgaatcgc tggtggacgg
     8941 aagaaagttt ttggacctgg caaagaagac agaaaaaccg attattgttc tgatgggcgg
     9001 ccgcagccag gaaggagcaa aggcggcgca aagccacacg gcaagcctgt cgggaaatta
     9061 taaaattgcc agcgccgcct tcagacaggc gggcattatc gaagtgtttg atccggcgga
     9121 actgaccgac atcgcacggg ctttcgccaa gaacatgcct tttcatcccg aaaaggaaaa
     9181 gggcacggct atcctaacgt tctccggcgg cgcgggaacc gtcaccacag acctgatgga
     9241 agactgcggt ctgaaactgg caaaattgag cgaccggacc ctcgcggcgg tcgcggaact
     9301 tttcccgccc tggaataaac ccgaccatcc gctcgatctc tggatcgcca tcgaacgcca
     9361 cggcttcgaa aaggtattcc ggaattctct gacggcggtc atcaacgatc cgggggtcga
     9421 ttcaatcatc tttcacagtt atgcctcgcc attgatcggt caggattttt ttgaagaact
     9481 tgctgcgatg attaagaaaa acaaaaaacc ggctgtgtta tggatcgaag gaagaaagga
     9541 tttcgccgaa catatccggg gtattgctga aaacgcaggc ctgcccgctt atcgcgaaat
     9601 gaaccgctgt gttaccgtca tgaacggcat gaaacgtcat ttcacgaaaa aaccgatgag
     9661 ctgaaaggag atcgccttgg cacgcatacc tgattttcaa ccgggtccgg ctgatacgga
     9721 aaagaaattt cttcagggca agcccccttt tgatttgaat attttcaaga tcatcacgca
     9781 cgcccccgtc gggattgcca gggagtttat cggacttccg gcatctgtgc tcatggcggg
     9841 gaaactcgat ccggttctga gagaaatggc catcacccgc gccggcattc tgtgcaattc
     9901 cgcgtatgag gtgcatcagc atcgcaaact cagcaaactg gtgggcttgc ccgaagaaaa
     9961 aattaaagct ctggataccg gttcagcatc cccggtcttt tccggcatcg aaaagctcgt
    10021 tttgcgcttc accgaagaaa ccgtcctgca gcgagaggtc agcgatgcaa cgttcgccga
    10081 aatctcgaga catttttctg aggatttact ggtagaactt tctatcgcgg tatgctgcta
    10141 catggcggtc agcattttcc tcaatacgtt tgaagtaaat cttgaagaaa atgatgtacc
    10201 cttttaacct taataattgt tttctttcca tcagtttcat tcatcttgac agcaattgga
    10261 atcctcatcc ggttttcctt ttattacgaa tttaaaaaac gaaaataatt tgaaaatatg
    10321 caaatttccc cgctataaaa aacggaatat gtttatcgta aaacgatttt tccgtaaaaa
    10381 cgattacagc ccaaatggta aaaatgtaag aaattgatat tattaagtat tattattgca
    10441 taaaccttgt gcatattggt ggaaattcgt aataattgaa cacttatggc gccagttaac
    10501 agggttatca acaggtcttt ccacaatatt gtggaaagac cttcatcacg gaataaaagg
    10561 ttaaaaatta aaaatatttt tgtctattta gattttgttg gacaataaat tcgatgaaca
    10621 gataaaaaaa ttatgcagca cacaaccatt gtaccggacg ttctgaaaca ttttctccgg
    10681 tcacgaacag tatcccttgc cttttacctt ctccaacagg tcgcattttc ctaattcaca
    10741 tgctttttgc gcatcattgc aaccatcttc tttattcata tgatcaaaat aaacgttggc
    10801 tctgttgtaa taggcatcca cgtaatccgg ctttatttta acagcttcgc tgaagtcctc
    10861 aacagctttc tgatattgac ctaaagcaag ataagcggcg cctctgttgt tgtggatgat
    10921 ggcactatcc ggtttcaggc ggatggcttc attataatct gcaatggctt cctgatgttt
    10981 cccgagttta ctcaaagcca atcccctgtt gttataaccg taaatcgatt gcggttgcag
    11041 tttgatggat tcattataat cttgaacagc ttccggatac tggccgattt caatgtaggc
    11101 gtttccccga ttgttgtagg catcggcata atccggtttt aatttaatgg cttcgctcag
    11161 atattcaatg gccttccggg gatcggtgca ttttccgctg atacacagtt caaatgcttt
    11221 gcgaaataat ccattcgcct catcacgggc gggtgcagca gctatcattc ggttatcgga
    11281 agctgccggc tgatttacag gcgcataaac ggcttgctgt tcgaccgcct gtgtgttgct
    11341 cacttcaact ttcttttcac tacatttttg tacgattgca agcagaatac cgacaatcag
    11401 gatgatcacg actatgaaag gatatttatt tctgttccat gtttttccga aaccggtttg
    11461 acgttcgatt ggagctttgc cttgtctctt cttccgcgaa tctttgacaa tcttaggctg
    11521 tttcgcctgc tgatccggtt gcggcggttg agatgccgac gctggcgtca ggggttccag
    11581 cacaacattg caataacggc atttttgagt tttatcctta aagcgcgtct ggcaaacagg
    11641 acatatcttc ataatgatgt ctccaacaga tatcctctaa ccatcgtccg aatatttctt
    11701 ctcccgtttt ggcatgtctg gattcttcac gttttaatct cgtgatgcta ccgttataaa
    11761 ttttcgggga caactcttgt tgccacaaaa tgacatccgg caaagcccca aacattgcag
    11821 gattaaaccg ttttccgaat aacaggaaat cgttgaattc ggatttaaat tgtatttggc
    11881 tattatcatt ttatgtggcc tatgaaaaga caaaaacaaa tattttttta atattcttaa
    11941 aataaataaa aaaagagtag gaataagtac aggtattgta cttattttcg ctgattatcg
    12001 agcatcgttt tgatttgtcg gatgaaatct gcgggatctt ttgttttcaa ggcgatgact
    12061 tcaccggggc agactgctct tttatcctgc gtcagaatat tggccgtgat gcccgcagca
    12121 ttcatgagga ttccatgatc ttttagaaat ctgtccgccg gagcggaagc aaccgttgtg
    12181 ttcactttcg cgataatgcc cgaatagacg accagtttgg ccgcggccag tccgatgatt
    12241 ttgtcgtgca gaatcaatcc ggattttgcc cggaactttt caagcgcctc gaaaagcgga
    12301 cgcaccccct tgccgtttga tgcatgaatt aacgcgcccc cctcgaataa agccagagaa
    12361 tattttgaga aatccgggat cagcataaca gttttcgcaa tggtttgttg atggtgctga
    12421 tcaaaacggc ccataaaatg tctgccagaa ataaaacgac aaccagtttt agaataaatg
    12481 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncggcccata aaatgtctgc
    12601 cagaaataaa acgacaacca gttttagaat aaatgcgttg gaaagatgca tgaaatataa
    12661 atccgaaaga accagccagg gaagctgaca gatgaaagcc agaaaaaccg agagaatcag
    12721 tttgtccgtc ttttgataaa acagtcccgt aaaaaaaccc aggatcgcgt tcccgatgat
    12781 caggtaggga ttgcccagca taaaagcgga atacagggaa ccggcaacgc cggcaaccag
    12841 tccgcccgtc gaaccgaata tcagagcggc cagaataatt gcggccggga aaaaatggac
    12901 cttccactgc tgcgcaaatt ccagattaat gagactcaac acgaaaggga agacaatcag
    12961 cagaaaaaaa acctccgcca tgcgtctgta tagcgttgtg ccgcttgctg tcgttgatga
    13021 atgcatcata tcgggcacct ctcgtttgtt atttttgagg tttagcttac actattatcg
    13081 attctttgaa cagactcgaa gatgattttt tgatgcaata aaaaacaaaa agatttaaaa
    13141 tatgttatcc aaataaggaa cggagacgga ccatgaaaga tttattcttt gaaccggtta
    13201 aaggcgggaa aaaaccttac cttgaatcgc tgagaaagct tcttcaacac ctccggcctc
    13261 ccttctccgc cggcgacacc gtcggcatta aattgcactg gggagaaaaa ggcaataaag
    13321 gtttcctgcc gcctgattat gcaagggaaa ttgctgcctg gctaattgcc cacggaacta
    13381 aaccgtttgt ttttgataca acggttctct attccggcgg acgccgtacg gccgaagact
    13441 cgctcataac ggccgcctcc cacggctaca cgcatgactt tctgggttgt ccggtgatta
    13501 tcgccgacgg catggacagc cgcaatcttg tggatattcc cgccgggttc aatcatttca
    13561 ggaccgtgca ggtggccaat gtttttgaga acgcgcaggg gtttgtcatc ttctcccatt
    13621 tcaaaggaca catggtttcg cttttcggag gcgccattaa aaatatgtct atgggtttcg
    13681 cgtcccgcgc gcagaagcaa cgcatgcatt ccgattatca tcccgtcctg aataagaaaa
    13741 aatgtacacg ctgcggagtt tgcgcggaaa tctgtccgac cggagcggca actatcgcaa
    13801 catccgacta tcccacatat gatttaaaaa aatgcatcgg ctgcgcccag tgtattgcga
    13861 tgtgtccgga aatggcactg cagattttct ggaattcgga catgacggtt tttcaggaaa
    13921 agcttgtgga aacggcggcg gccgtgtgga aaataatcaa ggacaagacc atcgtcatca
    13981 attcactgct gaatattacc gtggaatgcg actgtctccc cggtaagaat ccggtgattc
    14041 atgaggatat gggatttatc ggcggttatc atcccgtgcc cgtggatgcg gaatcgctca
    14101 aaatgaccgg cgcagatatt ttcgaaaaag tccatcccgg cattccctgg caacggcaat
    14161 tcacctatgc ccgggaaatc ggtttttctc ctgcccggaa catcaccgga ttttaaattt
    14221 gaaaaccgcc gggcgaatcc cgcttattcc gcaagaacgt aagcgaaaat cagaggggca
    14281 acaattgtgg catcgctttc aatgatgaat ttttttgaat cggggcggat ctttccccag
    14341 gtgatttttt cattaggaac ggcgccgcta tacgagccgt atgatgtcgt gctgtccgaa
    14401 atctgacaga aataggacca taaccggcag tccattttca tgtcctgctg aatcagggga
    14461 acaacacaaa tggaaaaatc tccggcaata cctcccccga tctggaaaaa gcccgtttcg
    14521 ctgtgccggg atgcatctaa ataccagtca atcaaggcat ccattgtttc cagaccggat
    14581 ttcacaatcg aataatgctt aagttgtttc ctgcggacac tggaaacaaa ataattgccc
    14641 agagaggagt cctcccatcc gggaacaaaa atcggaatgt ttttttcaca ggcggcaacc
    14701 agccatgaat ctttttcatc gatttcatac tgatctttca gcacgccgct gctgatgatc
    14761 tgcatgatga attcataggg gaagtaccgt ttattctcat cctcagcttt tttccagaaa
    14821 ctgaaaagct tgccttcgac cttccgcatg gcttcttctt cggggatgca ggtgtccgtc
    14881 acccggttca gcttgtttct cagcagttcc gcttcatcct cgggtttcaa atccctgtaa
    14941 tttggaacac gcttgtaatg cttgtgggcg accagattga aaagatcctc ttccagattt
    15001 gcgcctgtgc agcatacggc atgaatttta ccgctgcgaa tcattgccgc gagagtgata
    15061 ccaagttccg cggtgctcat ggccccggcc agtgccatca gcattttccc gccgttatga
    15121 atatgattcg cgtaggcctc cgctgcatcc acgacgacag cggaattgaa gtgtcgataa
    15181 ttgtgtttta tgaattcctt gacggattga aatttggaag tgtcgtgatc atacatttaa
    15241 taccccccaa accagcactt tcattggcac cttacaataa atgcaaaatg ttctcaatga
    15301 caaaatgaaa atataagtat ttttaacgta ttatgagcat cgtgtctaca ttctttatca
    15361 aacggaagcc gtttcaacaa agccggacac gggggaaggc ggcaaattca acgaatccct
    15421 atcgggagtg tttaaaaaga agatgatgag attgcagaac cggctttatt tttgcggcca
    15481 gtttttcggc tatttgaatc gtcccgaagt ctcccagatg caccatgtcc gggcaaagac
    15541 cgcccgcaac atccggcatc aggtcctgac cttctatcag atagagatta tccagcgcag
    15601 aattttccac ggctttccga agcaatttgc ggtacaggtc gggctttttg ctttcgatat
    15661 aatctcccag atacggcttg agcgtgatgc aaaaaaccgg acggcttttg ttgctgccgg
    15721 cgatcctgtt gatcatgtaa ctgactcgcc tcgaaaattc ttccggcgaa aatgtgtcga
    15781 ccatgttcag ggaaagatca agcgtcgcaa agctccagtc ctttcttccg gcgatatagt
    15841 cggccatttc cggttcgcaa tatgccgagc aggaagaacc cagattgata acgtccacgc
    15901 cgagacggca gccgaccaga ttcggatagc tcaaatgcga ttccgaagca taagctccct
    15961 gggtaagcga cgttccatag gccagatagc tcaggccggg caattcctca gcgtggggag
    16021 gtcttatttt tccttccgtt tcaatgccat gaaaccggat gggagcgccc atggtcgttc
    16081 cccaaaacct gattctacag acatggggag aaaacggcgc atcggtcgca attttctccg
    16141 ccagttcgat gaatttcggc ggccggatga tttccagcag ggttttgatt ctcttaatca
    16201 caaaacgctg cctcgtttga aaggttccga aaaaaacccg ggcatcggta ataattccgt
    16261 cgccgagatg ctttgtgatt ttatccacgg acagggtgat tttcacagga tacgtttcac
    16321 tgacaaaacg gatttccgaa ccggaaggac agatcatcat catttgtccg gctttgctga
    16381 gacgtgcgga aacgtgttcg ggaatacgtc ctaacagaag cccttcaata tctttgtgtt
    16441 tgtatagccc gtctacgtta tgaagagcca cattttcaaa aatcatccgt tctcccgttt
    16501 tcaggattga cttaattctt tatttgtttt cctggcgtct gaatttgaat tcaacgccgg
    16561 ccggtgtttt cataaccaga caccgattct cgttaatctg gagcatcgta tctccgattc
    16621 ccatttgaca tttcaacagg tccccctctt tgatacaggg gaaagatcct aaagcggtat
    16681 cgactttata agcactgccg cttttggtta tggtcagcat ttccttttct atcacgtcgt
    16741 tgaaccattt acccgaaaac gggtcgctca ttccgcacga taaaaggaag agggctatga
    16801 gcatcaccgc cacaacggca gattttctga taatcacatg gacctccttt ttcaaatata
    16861 ctgattgcga tgcccgttgt cgcagagcgt ttagctgata tcacatgcaa gttttttgtc
    16921 aacacagcga tcgcgttatt taacctgtaa cgtttgcagc ggcatttctg tatgcggcgc
    16981 aatatcatta ttaccgttgc gcgaaacacc ggatatttcc tgcaatgcaa cgaccgattt
    17041 catcgccttt ggttgcatcc atggcgcgta caatgaaata tatgcgcata ccttcctcat
    17101 cccgacaagt tttttttatc ctgtggcgtg ccggtcggaa tggcctcgcc ggttttccca
    17161 aatacgatgt tgccgtacgc catgattaaa atggtaatga atatgatctg cgtcaccacg
    17221 ccggtattca tatagggcag cgccatggct tgcggtatgc tcaaaaaaag taaaatggtt
    17281 atcaggcctc gcggtgcaaa ataaagcagc ggggtcattg aaatccggat aatgctcttt
    17341 aaatacaaag ctcgtaacgc atatattgcg gcaacaatca gaacggaaaa taataaattg
    17401 tttaaattca gcaaatcaaa aaggctcgta taatatccga acaggacaaa aaagaaggaa
    17461 cggacgataa aggtcatttc cgacgtaata tttttaaaag cattcaattc tgtgttgaag
    17521 tcttgaaaat taatgaactg agcggtgatg ctgttttcga aaagattatt gttgttcatc
    17581 accaggccaa aaatcaggac caccagcaga gacggcagat gaagcaacct ggacagcgca
    17641 tagacaagaa taatcgcggt cataatgatg atgtatttta catgatggtt gatcttatgc
    17701 aaaatgacag ccagaaatgc gctgaataac acagaggcaa acaatgtgac aatgatttcc
    17761 agaagaaaat tcagaatgcc tgaaaaaaga tgaccgttac tgatgaccac ataattgaat
    17821 gccagaatac cgaaaatatc agagatagaa ctttcatata tgacaaattc ccggttattt
    17881 cccgacaaac cgaccgccga tggaatcgct acggcactgc tgatgacggc aactggaata
    17941 atgttaacca gagaggtcct aacatcgtaa cccagaccat aggataacat aagcgcaaac
    18001 aagcccgtga ataccaaaaa aagaacaatg gctgaagaca tggacttgag gatcagggac
    18061 tttttttgtt tgctcagggt caaatcgaga ctggcttcca gaacaatcag gatgagccct
    18121 accgtcccca tgaccggcag gatcacatca atttgtgata cttcaaagtt aaaataactg
    18181 tcgatggaat tgatggccat tcccgtaaga atcagaagca gaacaccggg aatttttgtt
    18241 tgccgcgatg tcacgtcaaa aaaatacgcc agcaggataa cgacacagaa ggcaataatg
    18301 atgtaatagg tcattgcaga ggatcctttt tcgagcaagc gatgtggtga tttgcagtcc
    18361 aatcaaacgg gcgcacattc gtggacgtaa ttttaacgat tttacagtat tccttcttcg
    18421 gcaaagctgt aatagttccc gtctccgatg atgatgtgat ccagaacttt gatgcccatc
    18481 acttttcccg ccgccagcag atcctgcgta aattttcgat cttccgcact gggcgaaatg
    18541 ttggcactgg gatgattatg aacacaaata atggctgctg caaatttctg caatgcagcg
    18601 tgaatgatct cccggacaat cggtatggca tgattcaccg tgccttccgc aacgtcgctg
    18661 atgtcgatga tcttattgtt gctgttaaga tacaggactt taaataattc cgttttgcga
    18721 tctcgcatct gcggcatgac gatttcgacg acatcgcagc aggaggctat tttttgtttt
    18781 cctttcagcg cctccacttc gcgaaaacgc cggccgattt ccagagcggc ctgaatctgc
    18841 gccattttgg cgggtcccaa tcccttaaat tccttccatt cgcgcacatc cgtatgaatc
    18901 atgttacgga acgtgccgaa tttttccaca atccggcgag ccagatcgat ggcacttgca
    18961 cccttgacgc ccgttcgcaa aagaattgcc agcagttccg aattgctcag tgtttctgca
    19021 ccctgccgca gaagcttttc ccggggacgg tcgtcctctg gccaggaatt gatgcccttt
    19081 gaatgttttg attcagattg aatcacgtga tactccgtca gatacgtttt tattattttt
    19141 tgagcgcctt atccatgaaa cggtttatga tcagcatgac aagatgatac aaagggaaac
    19201 atgtcctttt gataaagtaa aataatttta catccagtga cgtgatgacc aggtacttat
    19261 ttttcaggat cgcctgaata atcaaatcag cgactttttc cggcgtcatg gcgattttca
    19321 caaacatctt gcgtgcttta ttggttgcct catgatcatc cgtgcgaatg tccgccgtat
    19381 tgactaaccc tgtatggacg gcacccggac agacaacgct gactccgatc ttgtactttc
    19441 ttaaatcata tcgcaatact tcggacatac ccaccagagc atgttttgtg gcggcatagg
    19501 ccacatgcca gggaagcccg ataagacctg ccgtcgacga tactgaaacg acatgtccgc
    19561 ctttccctgc acgaatcatc tccggcacga agcattccaa accgtgaatg acgccccaca
    19621 gatttattct gatgattttc tcccagtccc tatgttgcat gtcttctatt ttcgcaaaaa
    19681 gcgccacacc cgcaacatta accagaatat cgagcgaacc gaattccgca tgaatctccg
    19741 aggcaaacgc ccgcatggct tcataatcgc caacatcaaa cggacgggac agacacacgt
    19801 tgtcaccagc gctgctgatc atatccgccg tcttcttcag accttcagca ttaatatctg
    19861 tcagaaacag ccggcagccc gccagcccca tggccgcagc ggtagcacga cctattccgc
    19921 tggcggcgcc ggtaatcaat acatttttat tcgataacat cttcatttta tgcattgcca
    19981 cctgatagaa taaaaacgaa tgcgtcccat tcttccctgt cccacaacgt ctactgttgt
    20041 ttctgaatca aagccagcaa tctcttcaat cgatttctgg ctatttcttt gataaatttt
    20101 tcggtaataa atttccgttt ggtgattttt gcgcgatctt taataatatc catttcagga
    20161 ctgtccaggt tgctgttcag cacccacaga ttgtaacgtt ttttgcattc cactccctgc
    20221 tgtccgaggt aggcataggg aaaaattaaa accatgatgc tgtcactatt ataaatctta
    20281 ccggcgatac taaccgcgtc ctgcaatacc ggccacggca gcgtatgatc aataacaatg
    20341 attttgatac tattgaggag ttcggacata ttgacgccca gatccgacgt atcaacaatc
    20401 aaagctgctt tgattcgccc gtcatgcttc acggacagga ccctgctctc acgtttcagt
    20461 cctattttat tatacatgtc catgatattg ttcttattct ccgtcagaac ttcatgaact
    20521 tgaccggata attcggtttc tgccgcccga cattccaggg cataagcatc aagcatcatc
    20581 ccgccgccga tgccgctata atattcacgc atgagcgcca gatcatcgcg agaaaagttc
    20641 tctaaactcc aatcagacgg gaggccggtt ttctccgcat caacggtcat cggcatataa
    20701 ccgaaaagat ccatcgaaca tatttcccga cgcttaaata ttttgcaaac gccgccgaaa
    20761 taataatccg agaatgtgtt ttccggtctg tagtaacaaa atgtataacg catggtcttg
    20821 gaagagaccg gcaaacggaa caggcaatcc aaatagttga taaaataatt gagggtttta
    20881 agtccggtcc gcttcccttt tagaggacgc gcagccagat gatgaatgat ccatgaattt
    20941 tcgtatgctt tgaccatgga caaatgaccg taaacctgtc cttgatcctg gtaagtcaaa
    21001 tgcgtgaaaa tatcctgacc ttcttcataa aggcggcggt atgtctcttt cagcgcttcc
    21061 gtatacggac tgatgatgcc gtatttgtcg ggatagatga atcccgaatc aaaaaacagc
    21121 tcccagagag aatccatgtt gactcgcccg gtaaggttga catgcgggtc ggcggctttg
    21181 cttacggcat caaagagtct tttctgatcg ttcaacgaca tgtccagaat ataaaatccg
    21241 tatttcacca tgttcttttt caatttaagg cggtaaacca cttgagcggc gcagaccaaa
    21301 tcattgaaca ttcccggcag atgaagagtc atctgcggaa gaagcattcc tggaataagg
    21361 agcgattcat tatcggaaac cgttacggaa aaaccggagg ctgaaatatc gtcaaccttg
    21421 ttctgaacgg caagaccgca aagcggatgt atgaaattga tcaccggcgg cggcgttatc
    21481 ctgacccggt cggcggcgta ttttcggggg gataagaccg gacgattctg atgaacgggc
    21541 tgaaggataa tgtatgagcc ctcattatcg gaacgaagca tctgacactc gcccatgaaa
    21601 tgcgtctgat cgctctgact gagtttcagg aacaaatgat tggcgggatt aatgtcttcc
    21661 ggtgctctga tgcatactct taaatcatgg gggttgatgt cttccagaac accatgaaca
    21721 ttgagcgttc cctgaacaat ttccgcgtca acaccttcgc aagtatgacg cacggcctgg
    21781 cgcttgctgt aggaatgagc cacatcgggc atcttaaaaa caatggattc atgattgacc
    21841 gactgcaaat caatcggaaa aacgatcagg gatttacggt tgtctataat cagattcaag
    21901 ggatgatgcc gctccagatc gaatgccccg ggatcgataa aacggatggt caccgtatca
    21961 ttgagataag gacccggctc tccctgctgg agaatttcat cgccggtgtt cagatcacga
    22021 atgtgaatgg aaacctgttc ctttgtaaaa tgcaggtgat taatcagatt gattagaatt
    22081 tttttattta cttttggaga cgtcgccagc gctgacgata cgacacgggg atcaattttc
    22141 ttaaagggga gattgtattt gaatctgaac ttccgggaat cggaactttc ctccgcacga
    22201 ttggcgtctt ccgtcacatc taactgaaag gacggcaaaa tcgctgaata ttccgcatca
    22261 gccgtggata tccccgcatt tccgcgcacc gcataattat ttttctcgaa aatgtccgca
    22321 tagtattctt caagttgcca tgcttttttt aaaatactga caatacgtcg aggaggagga
    22381 cgtttaatta tattttgctg ccaatcatgt ctgaaatttt gaacaaactt catcatgaac
    22441 ttcctccatg caaaacattt tcaaatcatt attttttgtg ttcgataaga attttcatcg
    22501 ggtgtctact ctatcctctg cgacttctca caaaggcttt ctacctctgc acagaatcat
    22561 tggagaatat gtgaaacgcc gtggatctct gaaaaaaatt ctaaaatcat ctaaaaattt
    22621 tttactgccc cctgcgtaag actcaaatat ttccggcgct ttcagagcgc cgatttccag
    22681 tttcttcatc caattgaatt cgtccgaatg ttgaagtttg ccataaataa gatgatgcgc
    22741 cacaacattc agcgttagat ctttaaaacc caggtcatac agatgagcat aacatttgcg
    22801 tcccgcatag agatcaaaat taaagcaatt ctgcatatgc agcattgcct tgataaccac
    22861 gtcttccatc tcccgaggca tttcccaatg acaaagacaa ttatgatcaa gatccagaag
    22921 gcacatgtaa ccgccgggct tcaaattggc gcttatgttt ttgatgatat caaaagcccc
    22981 ttcgcgataa tattccagaa caaagcgtac ccagataaaa tcgaatcttc ccaaatctgc
    23041 catgggttga cggacatccc ggacatcgaa ttttatccct tttctatagc cgtagaatga
    23101 acgtgcataa agaatgcggc tttcagcaat atccacgcct acggcttccc cgtcaggctg
    23161 gactacatca tgtagcagcg ccgtcgtttt accgggaccg catcccacat ccagcactcg
    23221 agcaccgggc cccattccaa accacaacgc ctgctcttta accgcatccg gatctgtttt
    23281 aatatccaga cgtttttcct catcgctgtt ttccatcaaa tacgtttgtg tctgtgtcat
    23341 taaatcacct gattcatttt ataattattt ctcatcttaa gattattttt cacatctggg
    23401 gaagagaatt cgtttttaca tacttttttg cgaggccatg ttgttcgatg tataaaattt
    23461 tataaaggct tcaacaattt cacgactgaa atgaacatca ctttccctgc gcaacatttc
    23521 aaaggcttca tccactggta taggatcacg gtataaccgt tttgccgtaa tcgcctcaaa
    23581 aaaatcagcc actgcaatga ttctcgaacc aatgggtatt tcatgcccct tcaaaccgcg
    23641 cggataaccg ctgccatcaa gtttttcatg atgcgcttca gctatttcag gcacaagact
    23701 atatggcccg tgaaactcga ttttttccag tatttttctg gttttcacag aatgggtttt
    23761 aaccgtttcg tattcctcag cggtcagaag gccttctttc ttcaacaaag catctgatat
    23821 gccgatttta ccgtaatcat gcagcgacgc ggcaacggca acaacctccg tgtaatcaga
    23881 cggcatattc atttccttac atatacccac cgcgtattcg gttacttttt ccgaatgccc
    23941 cgccgtatag ggatcgcgtg cgtcaatact tgccgccaga gctttcagaa tagatttcaa
    24001 ttgacggttg cgctgttcat gcaattccgc gtttcggatg ctgacgccga gcacggaggc
    24061 aatgcccata agcaaacgga gatcactttc gacaagtgtc ttctgagtct tgaaattatc
    24121 aacagtcaga ataccgacgg actttgtatc ggaaagaata ggacagcaga taaaggactg
    24181 agctcccatg tccttgacga actgcagact tttctgtgat aacttccctg taatcgtact
    24241 gatgtcattg atcaggtatg gtttttgctc gcgaaaagaa gttacaaaca ctccttttga
    24301 ttgaggcttt gttaagtcaa attccgttga tttgacaatg gccatttgtt tttctgtata
    24361 cccgtaaccc gccttgaaaa caagcctggt tgcgtcttcg tttgaaaaaa ggataacgca
    24421 tctgtcatat ccaagtcttt tttccagtac ttgtattaca ttatcaagga tttcatttat
    24481 attgatgtat ttgttgatcg cctgaccgat ttcgtgaacc atttgcgaat tattataatt
    24541 aatttccatt tgctgaatca ggtcgtcgcg ggaatgttca agattcaaga tgctgtctcg
    24601 aagttctttc tttgtctgtt tgtcgtttag aaaattgatg atcagaatcg ttatgattat
    24661 gatcggaatg aaatatacaa gctcatctac cggatagatt attgtcagga taatggcgac
    24721 tatagccata actatcgcta tgatatttct tgttcttttt aacagatacg atcggttgtt
    24781 cccccatgat atgatatatc gacaacaatc acccccttta aacatacatt cgggatgttc
    24841 tatctcaggg atagcgttat taaaaaccag cgatattgac tcgaaatgac ccatcctgtt
    24901 ttcacactga aaaggttgtt cttgaactcc ctcttttggg gtaacagtga tttcaatttt
    24961 ttggggattt atttctctgg attcgtaaat cgaggaacgt gaaaactggc tggtcacttt
    25021 accgatcatt ttatagaccg taccgggatt cgccattccc aaaacgtact gcctcatgac
    25081 acctattgat tctggagatg cggcatagcg gccggcttcc cgcgctaaat tgcttgcacc
    25141 ggttagttcg tcaagttttt catggaaacg attgatttgt tcctgagaaa accagtgatt
    25201 ttgatcggca acttcgtatg gctctattcc agcataatca tagagctcat ctatgtttat
    25261 atattcgtaa cactttttta tatacttaac ataggaattc acaattcggc tgttgtaaag
    25321 aggttttgaa tccaaacaag cactccctct gggtaaataa atattggcac tttcttgatt
    25381 tacgcgggag tatataaaaa agctcgtcgt ctgtcaacga aatacagacg cttggtgatt
    25441 atatcctgat taagcataaa aggaatctat tcgaaaaata attcaaccag aaccggaaga
    25501 aaggtcttat ttgttccttc gcatggacgc cagtttggtc tgcagatcat cgatgcgttg
    25561 atgattatct tcggaaccga tgtaagtacg gaacaaccat tcctgttgac gcatggcctg
    25621 atccagactg gaattcagaa gcagatgcgc cagttgcttg gtttccttga ggcagccgcg
    25681 atcatacgtt gccatctcac gggcaatggc accgacttct ttcagaagct gcccttcggg
    25741 gaaaatgcgt gagatgtacc ccatgcgtaa agcttcctgt gcgtcccaaa tgcgacctgt
    25801 cagggcggat tccatcgcat tgctcaagcc tacaattcgc cagagcggat ctatggtggg
    25861 attgagagaa agaacgattt cccgttgtcc gaatctggca cgttctgaag cgtaacggat
    25921 atcgcacatc atggtcagat cgaacccgcc ggcaatggcc ggcccgccga cagcgcagat
    25981 caccggctgt tcgcaaaaca tgacggcgcg ataggcccgg tgaaacaggg ccgtgtaggc
    26041 ttcgttatgc gctttctgca aggtacggat gtaggtcaga tcaaaccctg cagaaaaata
    26101 acgttcgccg ccggtcaaga cgacgacatg gacatcctgg cggcgcgaca gttctgtaaa
    26161 tgtgtgttct atgtcttcca aaacctgcgg tgaaagtgaa ttgcctttat ccggcctgtt
    26221 gatggttgcg gtcgccacat tttcctcaac atcaagtaac agatactgcg ctgtcataat
    26281 ttttaccttc ctaatttttt attttttatt ttttcgtctt cgacggcact gcgggttttg
    26341 attttcttgc cgaagctctt ttcatttttt tctgcgggac cggttccaaa gccagcttga
    26401 taatgtccat catatcttta acgaaatgga aagatatgct tttacgtacg ttgcccggaa
    26461 tatcttcaag gtctttacga ttccactcag gcaaaagaat cgttttgata cccgcccggt
    26521 aagcagccag aaccttttcc ttgatgccgc ccaccggcag tacggcaccg cgcagtgtaa
    26581 tttctccggt catggccagt tgctttttca ccttgcgatt ggtcaaaaga gaggtcaggg
    26641 ccgcaaacat agtcaccccg gccgacggac cgtccttggg aatggcgcct gccggaacat
    26701 ggatatgaat atccgtatcg tcaaaaaact ccggtttaat attcagggca taagcattcg
    26761 tccggatgaa gctcagcgct gccgccgccg attccttcat cacatcgcca agctgaccgg
    26821 tcagcgtcag tcctttcttg cctttcattg ccgtggcttc aataaaaagc atatcgcccc
    26881 cgaccggcgt ccaggccagg ccgatcgcaa tgcccggttt agcaatgcgg gcgtcaaggt
    26941 cggtcataac ccgcaccggc cccagatagg ccgggatatc cttttcctcg agcgtgccgg
    27001 atttcacggc gccttcggca atccgcgccg ccacgccgcg acaggcgttg gcgatttctc
    27061 tctccagatt gcgcacgccc gcttcacgcg tgtaccccgt aatgatggcg ttgagtgctt
    27121 tgtgagtcaa tttgaattgc gacgaattca gcccgttttc tttcaactgc cgggggatca
    27181 aataccgttg ggcgatcttc actttttctt cctgcgtata accgttcagc tcaataactt
    27241 ccagccggtc cagcaacgcc gggggaatcg tatccagcat attggctgta gcaataaaca
    27301 taaccccgga aagatcgaac ggcacatcca gataatgatc cgaaaaagta ttgttctgct
    27361 gtggatcaag cacctccagc aacgccgagg acggatcgcc tcgaaaatcg ctgccgacct
    27421 tgtctatttc atccagcatg aagaccggat tgttcgattc cgcccgtctc agtccctgaa
    27481 tgatgcgacc gggcagagca ccgatataag tgcggcgatg gccgcggatt tccgcttcgt
    27541 cgcgcacacc gcccagcgaa atgcgcacga acttgcgctc cagcgctctg gcaattgact
    27601 gtccgagcga tgtcttgcct gttcccggag gtccgacgaa acacagaatc ggtcctttgg
    27661 aatccggctt gagtttgcgc accgccagat attcgatgat gcgttttttg ggtttggtca
    27721 gaccgtaatg atcttcatca agcacccggc gagccttgcc gatgtcaaga ttatcgtttg
    27781 tcgattcatt ccacggcagc gtcgtcatcc agtccagata cgtagaggaa acggtgtatt
    27841 ccgcagacga cggatgcatg cgtgacaatc tctccaattc ccgcaaggcc tcctttttcg
    27901 cctcttcagg aagatttttt tcttcgatct ttttacggta ttcttccgtc tccacggcat
    27961 tctcatcggt ttctccgagt tcctgccgga tggccttcaa ctgctggcgc agatagtatt
    28021 cgcgctggct cttatcgatg tcgtccttga ccttcgtctg aatcttgttg ccgagttcca
    28081 gaacatccat ctgatgattg accatcagcg taagttcttt cagacgtttt ttgatgtcgg
    28141 caatattcag tatcttttgt ttttcctcca ccggcgcatt gataatcgac gcgatcaagt
    28201 cagccagcgt tccggcttca gtaatggatt tggccatcgg accgaattcc ggcggcagaa
    28261 agggtgaaag cttcaggatg cgatcaaaca gggaaagcag gttggccatc agtgcttccg
    28321 tctccagatc tttggcatct ttctcttcca gaagtttaat gcgcgcctgc tgatacggtt
    28381 ttccttcaat aatttcttct atcgaaaaac ggctgacccc ctgcagcagc agttgtgtgt
    28441 gattatccat ggttttagcc atcttcatga tcacggcaca ggtccccacc ggatgcaaat
    28501 catctttctt atagtcgttg ggtttgtccg gttcctttct ggacataatc agaccaatca
    28561 ggcgatcccg agtcatcgct tcatccacca gcaccgaagc gctgcctgtc acttccagcg
    28621 gaatcatggt tttgggaaac acaagcacgt tatgcaacgg aagaatgggg ataatttccg
    28681 ggatattaaa agtgttctta ttttgttgcg ggacagtttg ttccgtcatt tttaacctcc
    28741 agtgtgattt tccgaaaaga gattttcgct atttttccga aatgatggaa atgcgatgcg
    28801 acttgctcac gggcagcttg ttgattctga ccattaaaat tccgtcggca taggacgctt
    28861 tcgccgactg ggattcgacg ggagcaggca aagaaatgat tctttcaaaa taaccgtgcg
    28921 gaatttcggc ctgacagtaa tgtgcgtttt gtaaatgctg aacggccttg cgcactccgg
    28981 ctatcttgat ctgctttcgg ttcacctcga tgtgcagttc ttccttattg agtccggcca
    29041 tgtccgccag tacaatgatt tcatcggtgg attcatacac atccacattg ggacgccaga
    29101 tgcattcata gttgtgaaag gccggcctga ccagatgaaa tacttcatcc accactttct
    29161 ggaactcgtc ttcaaaatta ctgccgaaat tgattttaat gtacgtcatg ttgctgcctc
    29221 acaaatctgt ctctatactg gttaaaatat aataatgccc tgacgacttg taaataccgc
    29281 ctcaaaaatt cttccgtttg acatgtttgt actccagtcg actccgctag ggcaagaata
    29341 tcgtttgcct ttttgaatca tacacagccg atctataaaa tcaaatccgg atttggacac
    29401 tttaaaaacc atgccaaaat taaaatcaac agacacagag aggccctttt acatcatgtc
    29461 ccggagccat gcagcaatcg gcggcaccac gtcatccacg ttgttggtca tcaccagatg
    29521 ctcggtgtct ttaaaaaaca gatatttctt cggacaggtc aagcggttga aaatgtcctc
    29581 aacgtaagcc tgcgggaaga tggagtcccg ttcagcatga atcagcatga caggcacctt
    29641 gatttgttcc accggtttgg ccagatccgt tttcatcaac gaccctagcg ctctgagcgt
    29701 aatccatttc acggtgaccg gattattttt gatcagcgtt ttggcatccg ttccgcttct
    29761 ggatttttcc tgcgtcaaat ccagataaag aaaaaccgga acggaaaaac gtttgtacag
    29821 gtccgccaga aatccggcaa taggcgccat ccataccggg ggcctgatct gagaaagaat
    29881 cagattgtcc ttcccgttca gatcagcgag attgtgacat acggccgccg caagggaatc
    29941 atcgcgcgct gccgcgtaga aggcggcaat accgccctgg ctgcttccga ctacggcgac
    30001 agggccgctg aatcgttttc gtgcaaattt gacaaccgcc agcgtatcct caacaatttc
    30061 attgacggta taatctcccc ggagaccgct actcatcccg tgaccgcgcg ggtcaaaacc
    30121 gactacattg aatccctgaa gataaagttt atacatgctt tcaatataaa atttggagta
    30181 cacggacgtg ccgggaataa aaacaatcgt cggcccgttg ctgcctttcg aaaaaacatc
    30241 cagatgaatc cggatgccat tggcttcgat ggacatttct gtaaaggcat gacgatcgat
    30301 gctgatttct tcgtctttta aaagtttatc aaacgctgcc tgagcggttg cgtcatcggg
    30361 aattttttca tgctgaaccg aacgactgac cgcataccag gcgatatgaa acaaaacggc
    30421 catcaacagg actaacataa taattttttt tgtcatcgcc actgaacctc cgcttttttt
    30481 ctggacactg tatttttata gcgatacgcc gggtaaccca tgaccagcgt cgcatagacc
    30541 ctgcggttat ccggaacgcc gaatgttttt ctcagccggc cgctgaaacg cagcatcagg
    30601 gtcatcatgc ccacaaaaca ggtccccaga ccgagcgtat gtgcgtaatt gatgatggtt
    30661 gccgcggcaa tattggaatc atcgcaaacg aacatttttc ttttcgccgc gtgaatcagt
    30721 atcacgacag gggcatgatg caaaatgtaa tccctgcctt tttcggcgtt ttgcatttgt
    30781 acgtacgcca tcagattata ataagggttt ttgttcaggc cggtgagatt gattatccgc
    30841 cggccgatgc ctgatgttgt catgttatac aaacggtttc cccagccgaa taaatccgcc
    30901 gccgtctttt cgatcagcgg cttatccgtc acgacaatgt agccgacatc ctgatgattt
    30961 ctcgccgtag gcgcataccg gaccagatca agaacttttt cgattaattc gcgtggcacc
    31021 ggttcgtttt tgaattgccg gatgcttctg cggctcatga cgatgtcgcg gtagacatcc
    31081 ggattcaggt tttttctgtt gatcccggcc agttcttctt ttctgagacc gtcgtgaaca
    31141 atggcgtcac aggggcatat cgcgatacag tgactgcagc cattgcagag atcaaccgcg
    31201 accggtctgg attgaccgtc gactacacgc catacgccca tcacgcagtc atggacgcaa
    31261 agcatgcatt cactgcattt atcctcgatt ttttcaatac gcattcaatg attccgcaaa
    31321 gacgtctgct gttttgatga gattatttct gttttgagtt ggatatataa agaagcggca
    31381 ggcatgtcaa taaaatattc gttgccggat tccggacagg cggcgttttg tttttgcctg
    31441 ttgcagcggc tgattgatca gacactgcaa caggccggct tttctaaaat caattttggt
    31501 attttaatca ggcagatatt taaacgacac cctcagctta acgcggtatt caacgacttt
    31561 cccgtcttca accctcatat ccagttcctt gacttccgca attctgattt ccttcaagct
    31621 tttcgctgtc atttcaaccg cctttttagc ggcctcttcc catgatttct cactgccccc
    31681 gatcacttca ataactttat aaatgctgct catttttgcc ctcctttcgc ctaaatggtt
    31741 taaaaatatt taaacatttc acggcggctt tccggccgtc aatcctggat aacgcactgg
    31801 gcaatccagt aagaaaaaac acaacgttca tacccgttta cagatcggaa aacacaaccc
    31861 ggcagcaaaa caaaagggaa atcacagaaa gggataacag attatgaatt tttgttaaat
    31921 tataattcat ttttaagaaa cgtcaaatga ttattataag ttctgaatat tcaatccata
    31981 atttaatatt catgtttcga ttgaattttt cataaaaatg acttagagtc acaaaccgta
    32041 aacgaaaata tttatttctg aagaaatccg cgcgcgacct caacgtccgc gtgaccatac
    32101 aacaaggagt attttatgca caaattcaca ttggacaagc ccgacaatct ggtcgattgg
    32161 tgggaagaaa gcgtgaccaa attttccgag agaccgctgt tcggcaccaa aaacaaagaa
    32221 ggcgtttata aatgggcgac ctacaaagaa atcggcaacc gcattgacaa ccttcgagcg
    32281 ggactgaccc agctgggaat cggcaaagat gatgtcgtcg gcattatcgc caacaatcgt
    32341 ccggaatggg cggtgatcgg gtatgccacc tggggatgtc tggcccgtta cgtgcccatg
    32401 tacgaagccg aactggttca ggtctggaaa tacatcatta acgacagcgg ggcaaaagtt
    32461 ctctttgttt ccaatcctgc aatatatgaa aagataaaag attttcagaa agatatcccg
    32521 acactcaaac acattttcat catcgactcc gatggtgaaa attcgctgac ggctctcgag
    32581 aagaaaggcg ctgaaaaacc ggttgcgccg aaaagcccca aagcggagga cgttgccgaa
    32641 ctgatctaca catcggggac aaccggcaat cccaagggcg ttttactgat gcatatgaac
    32701 ttcaccagca attcacatgc cggcctgaaa atgtatcctg aactctatga aaacgaagtc
    32761 gtttctctca ccattttgcc gtgggcgcat gttttcggcc aaaccgccga attattcgcc
    32821 atcacgcgcc tgggcggacg aatggggttg atcgagagca caaaaactat catcaatgat
    32881 attgttcaaa tcaagcctac ctttatcatt gccgtcccca ccgttttcaa cagaatttac
    32941 gacggtctgt ggaataaaat gaacaaggac ggaggactag ccagagcgct ctttgtgatg
    33001 ggcgtcgaag ccgccaagaa aaaaaggatc ctagcggaaa aaggccagtc cgatctgatg
    33061 acaaacttca aagtggccgt tgcggataaa attgtcttca agaaaatcag ggagcggatg
    33121 gggggtcgaa tgctcggatc catgaccggc agcgcggcca tgaacgttga aatatccaag
    33181 ttcttcttcg atatcggcat tcccatttac gattgctacg gcctgacgga aacatcaccc
    33241 ggcatcacca tgaatggctc tcaagcttat cggatcggca gtgtcggcag acccatcgat
    33301 aaggtcaagg tcgttatcga ctcttccgta gttgaagaag gggcgaccga cggtgagatc
    33361 atcgcttacg gaccgaatgt catgaaaggc tatcacaacc ggccggaaga caccaaagcc
    33421 gctctcacac cggacggcgg tttccgcacc ggcgaccgcg gacgactgga caaggacgga
    33481 taccttttca tcaccggccg catcaaggaa caatacaaac tggaaaacgg aaagttctgc
    33541 ttccccgtct cgctggaaga aaacatttgc ctggcttctt ttgtccaaca ggcggttgtt
    33601 tacggtctga accgtcccta caatgtctgc attgtcgttc ccgacttcga cgtacttttg
    33661 gactacgcca aagaaaaagg gttaccaacg gatatcaaca cgctggtcga acgagaggat
    33721 attatccata tgatcagtga agccgtgacc ggccaactca aaggtaaatt cggcggttat
    33781 gaaattccca agaaatttat cattcttccg gaagcgttca gtctggacaa cggcatgctg
    33841 acccaaacga tgaaactcaa gcgcaaagtt attctggaca agttgaatga ccggattgaa
    33901 gcgctttaca aagaagacaa ataatccgat aatgtttcca gacctcccgt aaaaagagaa
    33961 aggcgtgttt tccggacacg cctttctctt tggtttgctg atccgtctct ccgtttatcg
    34021 attggccggc ctctttccgg aaagaatgct gtcaaccgtg ttctgtttgg aataatgccc
    34081 gcgcgtaaaa aaatgccttg catcttcccg gcccagaact ttttgccgga tgcgttgcgg
    34141 tgacagtccc ctgtcgcgca gtctgaaaac atcatcccgc aaacgctcca tggttgaaat
    34201 cttttttgcc agggctattt tgccgtcttg cacaacgccc ttgaggttgc aaaaaatagt
    34261 ttctatgttc agggcactga gttttttcag tgtgtccagt atctgcaagt aattttcgtc
    34321 ctgccttaaa tatacgaaat ttgttccgca aaaaagatcg cccgtgaaaa gccatttttt
    34381 atccggctca tataaacata tgtgatcctc ggcgtgcccc ggcgaatgaa tgacttccag
    34441 atgataaggt cccgccttta cagaggcgcc gacagcctcg ccctttgacg gtctgggcca
    34501 gtcccacacg atccgctgat acagatgcaa atcattcaaa cggggatttt ccaaatacgg
    34561 cagcgtcagc ggatgcgcgt atatattgat cccgaagtgt tgctggacat cggcattgtt
    34621 gccgatgtga tcttcatgat gatgggtgtt gatgatcccg gcgatccttc tgcccttcaa
    34681 cgcttcaagg aattcatgcc ccgcacgatt ggtaccggta tcaatcagaa cgtcgccgac
    34741 taaaaaagca taaacgggat acagaaggat tcccaggggc gaccgcccca tatagaaaag
    34801 aaggatcgat tcctgttcgc tgatcttaaa caattttccc caccgcttag acagacgcta
    34861 cagcctgtta aggttttggt aaagtctatt caggaattgc agacagggcc ttgcctgcat
    34921 agtctgcata atagagactc ctccgacgaa gcgatgtatc cttgcgtcgg ggcgaattgg
    34981 atgttgcaac gcactcgatt tctcccagtt cagtgatgac ctcttccgtt tcggggaagg
    35041 ttacggcaaa cccgcattcg ccaccttcat attcaacact ggccttgtag gttccggggg
    35101 aaatcgtgct gcctccggct tttatcatcc gtgtaatagc atggcaactt tgcttatccg
    35161 cgacggtgga ggatccggca gcatcatctt cgccaaaaga attctccaga tagaattctc
    35221 cgcctttccc ggtaggagac aataattctt tttcaccgat cttcatggcg atctcgacat
    35281 attccaacgg aattttcttt ccttcttttc tcgcaaagat cgtccccgta accgcgcgca
    35341 actgctgggc atcgaaataa atacaggaac cgctccacaa agaaggagac agggtcttgt
    35401 tgacatccga tattgtaaaa tcaatcggaa tatttttcgt gtcgagagta atcttattct
    35461 gaccgtagga agacatcgtg ggtacaacca ttgtgccgag agaacccact ttcccgattt
    35521 cctgaccgtt gttgagcact gacgcacccg gaacgttttt attgaaaagc accacgccga
    35581 aactgtcgct gaccggccgc gaaaggccca gatattcccc ggcccaaacc atcgaccccg
    35641 ccgccgacaa attatacgat tccgttgaga ttccgttttt agaatcctga atctgggcat
    35701 caaaggaata aatgccgtag cgggcgttat actgaacgta cgggttggtc gtatacacgg
    35761 tattggcggc tgtttcggaa cgattaacgg aagcgcggta tcccagccct tctccaaccg
    35821 gaatgtcttt ttgcacttgc agcgtttcgg agtttacatc gtcgcttccg aaattcacct
    35881 gtgccgaacc gcgaacgtta tccattaaag caaagtttaa accgataaat ccgctgtagt
    35941 tcgtatcttc ccctgttgcg cgtgtcgccg aaccggtcgc aaaaagactg atagatctat
    36001 acagtgtccg ggagtaattc aatgatatga cttgcgtatc catcccttta aaattttccg
    36061 tctgagcgta attaacggag aaacttccca gcggatttaa agaaaagccc atgcccgctc
    36121 tgatttcgtg gcgcgtatta tcttccgtcg gcagcggatt gccgatggtt gcatagtatc
    36181 gcgaatactt gcgcagcagt aaattcgtac tgaaattccc cagttgcaaa ttgtgctgca
    36241 gagagaccgc atcgcctctt ttgtcgattt catccgaaga acttccggac aactgcacgg
    36301 taaactgtcc ggcaagggga acggcaaagg ttgtggagat gcctccgtta taaacgccat
    36361 ccgatccttc cgcactcgca ccgatattca ctttattggt cacgccatag cggtgaaaag
    36421 cggaaaaggc cagatcgccg tactcattgc tttctgatcc gtactgttcc cgaaggaaac
    36481 ctgcattata gctgtattca tgcaagccct tgcgcagcat ctgagcgctg aaatacgcca
    36541 tataggaaat tcgttgaaca tttccgaacg gatctttcag aacaacttca atgttgtgag
    36601 agcctgtctg ggaataaata ttttttaaat cgaatgatcc gggggcaatg tcctgtttcc
    36661 cgatcaggac gccgttcaga tagatttccg cctgtgtggg ataaataact gtgccctgaa
    36721 gatccatgac gggttgcgtg ataaaatacg gatcgagtct gtacaccttg gaaaagccga
    36781 tgcctcccat attcaccgta ctgcccaatt ctccggaatt ggcgtactgg tcccccatca
    36841 ccagccattg caaatctttc cggcgctcat acgtcgcact gctttgaagc cgtacaagat
    36901 cataatcgga ttccgttttt gtaaaaacag aatccgatgt gaaaaacata tctccggttc
    36961 tgaaccccag tttgttcgtg accgacaacg tctgaaatcc gccctgaccg gtaaaagcgt
    37021 agttcacccc gtaattgaca aaggcgctcg tctctctcgg gtaatagata tttttggttt
    37081 cagcccgcag ggaaaaaaga tcggcagcgg ttctggtcga ttccgtggtc ttgccgatca
    37141 ccgttactgt gagtttcttt tcgtcgaatg tggacgtcac atccagaaga gcgttcaaag
    37201 gaacatgcgg ctcatccccg cgcagaatga cgatccggtc ttcggaatat tgaagtttca
    37261 gagctttggc atcgtccggc ctgatataca gattaccgcg atcatcgagc tcagcaaaga
    37321 tatctcccct ggactgcgta ttgataataa cactgacgac aatcggggtg gtttcttttg
    37381 gagtgaacgc agaggatttc ccggattcgg caggaacctg tcgcgcactg cgagccgttt
    37441 cttcggccgt atatgccggt gcggcaaaaa aggttatcag aaaaaacacg accgcaacaa
    37501 gaaaaaatgt cttctcgcac gatccccgcc gcgataaata tctggttgaa aaatccgtaa
    37561 agttattcgg gacacatgtt cttttgaaca ttcaatattc cgctgatgtc tttgtcttcc
    37621 gttcggacat tgatgtcgat tttggccagt tgtccgcata cgtctttggg aatttccgct
    37681 tcgtaagagg atgacatccc gtttaagata taccagccgg ccacttcctt ggaatacaac
    37741 tctttcccat cggcggcttt cccgctgaac gtgactgaac gcagtttcac atgaacattg
    37801 ccggtgtttt tgacaaccgc ctttgccttc ccggcggcca tctcgacttt gtcgatcgca
    37861 tatatttcct gcggcttgag cggtttgacg aaaatcggca tggagaaccg gaaggcgatg
    37921 gtcacaccgg ccttgacctt ttgattgacg tcaatatcct gtgttttttt ctgggtggga
    37981 atttcctgaa caaaaagcct gtatgttttt tctctggcgc cgggcggtgt tttgagtccg
    38041 attctaaccg ctctttgaga attcggatcg atactcataa ccttcgggaa gaatacaata
    38101 tcatttgtat cggtgtaagc atctttactg ttctcgtcct gattccatgc tttaacggaa
    38161 acctgaaagt cgattttctc gttactgtta ttaatcaccg agaaaacccc gcttttcact
    38221 tttccaccta aatccattgt cgtcggttgc acctgaaagt cggcggccaa tgcatgcgta
    38281 gagtagatga ttacgaacaa tataacagcc aggaatccga caattttgtt catgggtgga
    38341 tcaaaatttt tacaagtttg gagcgatatc cttgtggccc ggtaattatt tttcatcata
    38401 taactcccga ttattttatt tttacaccca aacgctgttt ctcaaataac acagattgac
    38461 ctgcactgca agccgcagac cttggcctaa tatgcaatcg taactgtgac attgtccgaa
    38521 taatccccgg gcggcgcact ttgatatgct gacggcacaa ccgatatccc aacgggaatt
    38581 tccgatgccg gcgccgcgcc aaaaccattt ccggttattt tccccgaaac cccgcaggtc
    38641 gtgacattgt aattaatgtt atccgttcct tttttcaatt ggcctgccgt ggagccgccc
    38701 gacgtttcgc atgtcacatt gggttgtgac gcattggtgc atttgacggt cggcgacgtg
    38761 ccgttggcag ttgtacttgc cgtaatggtg gtattaagag acggatcgat gtcgaaattc
    38821 atcgtgccgt ttgtcggcgt accgcaggcg ggaatcaccg tgacatccga aacattgaca
    38881 taggccgaaa gcgaaccgcc ggcccagttg atctcaaccg ttatcggatt gctgaaggaa
    38941 gagacgcctt caacaaaacc ggcgtagctg gtaattgccg aatttgtggt cagcaaagga
    39001 atatccacag ctcccgctcc cataccgttc gcatgcaggc ccaacgtaaa cggaacggta
    39061 tttgcgccgg tcaactgtaa ttgacccacg ggatttgtaa ccgcaataga atatgccaaa
    39121 ccggcgctgc attgaaaatt aacctgctgg gtgattatat tattgacggg agccggataa
    39181 cctgaaggat caagatttcc gaatgaaatg ccccccgtat cggttccgtt caaaacgcag
    39241 ccggccgcgt taatcacgat tgtggaaaag catattggat ccataccggg ctgtctgtgg
    39301 ttaatcgcaa accagtaagt cccggcagtg gtcggcgttc ctgcaagggt gacggaattc
    39361 agggattgat taattaaact caatccggga gggagcgacc ccattacgat ccgccaccac
    39421 ggagacccgc cgtttccgtt tgttgctgta aaggtatagg aataagcaac cccgcgagtc
    39481 ccttccggaa gagttcccgg acagttaatt gctgcatgca cagtggcgga aagagtcagc
    39541 aaacaaaaca ataccggaat cacacacgca attttcagat tttcaaaccg catctttcga
    39601 tacctttttt cttattatat tagaaaaagc aaaccgcagc aaaaatattt ttcagacgga
    39661 tattctgcac aaagggattt gaacgatttt ttgaaactaa taagtgatgg tcagtgtcac
    39721 cgtatccgaa taatcattat gggcgacagc ggcttgagca tcggctgcgg caattttgat
    39781 gccgatgccc aatgccttat tggtgctata cccggcgcct gtaaaacggc cgttgccatc
    39841 cgtatcgccg ccacacgtaa aggtatagct gatcacgtct gaaggccagc tggatgattg
    39901 aagagccata tcacccacgc cgccggacgt gcaggtttcg tccagcgccg tcccgttggc
    39961 gctggatatt ttaacggtaa agaccgtgcc gctggtgcag cggaccaatt catcggcgct
    40021 gttcggaaag gtctgctcgg ccgctgtctg ggtatcgatg agcagcggcg atggaaacga
    40081 tcccttctgg atttccctgc aggacacgcc tacctgcgca ttgactttta tctccgctga
    40141 tgtaaaaccc gcaggactgg ccgcaaatac cggatttatt gcgaagctca tggcagccag
    40201 cgctgccatg agcaagataa tttttttcat tgacgcttgc ctttacgtca gtttttttag
    40261 tattctactg taattgtcac tgtatcgcta taggttcctt cctccgcggc attggcatcc
    40321 actatcgcaa ccgtcgccgc attggcgtta agcagcgtcg tcggtgttgc agaaccgaaa
    40381 ccagcgccat taaagctgct cgtataagcc agcgtatagg gtatggtagc acccgggccc
    40441 gttaacacac cggccagaga gccatcattg tcttccgcac cacccgcgcc tgcacttgca
    40501 gcggttactg taaaagcttt gctcttcgaa cattgtaccg tggtagcgct accggtggaa
    40561 gtga
//
LOCUS       DAOOLB010000015        39441 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_769_length_39441_cov_46.854288, whole genome shotgun sequence.
ACCESSION   DAOOLB010000015 DAOOLB010000000
VERSION     DAOOLB010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 39441)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39441)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39441
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_769_length_39441_cov_46.854288"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(65..808)
                     /locus_tag="PLF77_03530"
     CDS             complement(65..808)
                     /locus_tag="PLF77_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07958.1"
                     /translation="MMAKMRVYIGFDDTDIVEADYGTGKVARRFEKVLPAQCKIWGVV
                     RQQLLVDPAIPYTSHNSSACVVVDCPDSSYVDALKSAAIKHIEAMSLPGSDPGLSVVV
                     EGDLALPALEQFGRQCTARVVTLKDAVKAAGSAHLSGHGGTNGGMIGAAAAVGLTASG
                     WSGRFIEYDGLRDFPDEVIVAALMQKNIRVISLDRDACCPRPEDMVNTKGWLRPRLWG
                     HDVVLPVKPAGAGLWESLGEKRNPDKKKK"
     gene            1004..1456
                     /locus_tag="PLF77_03535"
     CDS             1004..1456
                     /locus_tag="PLF77_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPC07959.1"
                     /translation="MKTDEYKKIIMFAVDNEIEAYEFYKAVADKTKDGGLKKIFTELA
                     QEEAGHRKLLEGLASGKGQMHFDESKDYKVAESINKPKLSMSMKPADAIALAVKNEQE
                     AMEMYKEMAGCSTDAEQKKMFQSLANMEQGHKVKLEDTFTNMAFPEAW"
     gene            1860..2177
                     /locus_tag="PLF77_03540"
     CDS             1860..2177
                     /locus_tag="PLF77_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPC07960.1"
                     /translation="MTKSLKFIGSSLDDLRDFPDEARRAAGFELRALQNGLEPRDWKP
                     IVSVGPGVKEIRIHVLGEWRVMYVANIRDAIYVLHAFQKKTQKTGLRDIELARKRYKQ
                     IGE"
     gene            2181..2465
                     /locus_tag="PLF77_03545"
     CDS             2181..2465
                     /locus_tag="PLF77_03545"
                     /inference="COORDINATES: protein motif:HMM:NF025120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPC07961.1"
                     /translation="MKKSIVTSSDNVFIDLGYSKDEAAIMQMRADLMADLRRFIKNKK
                     LTQTEAAKIFGVSQSRVSDLITGKWEKFSLEMLIILVTKAGMNVKLKTAA"
     gene            2532..5282
                     /locus_tag="PLF77_03550"
     CDS             2532..5282
                     /locus_tag="PLF77_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07962.1"
                     /translation="MPLSWNEIKTRALAFSKEWQEETSEDAEAKSFWDGFFNIFGISR
                     KRTASFEEPVKKLGEKYGYIDLFWKGVLIVEHKSRGKSLDKAYSQALDYFSGIAERDL
                     PKYVIVSDFARIRLYDLEKHEQHEFDLKDLHKNVRLFGFVAGYQTHKIQEQDPVNIKA
                     AEQMGKLHDRLKESGYTGHSLELYMVRLLFCLFAEDTGIFEKQQFKEYVEQRTSEDGS
                     DLGHHISTLFQVLNTPPDKRQKTLDEQLAAFAYINGKLFEEMLPNAAFDSAMRQELLN
                     CCALDWSRISPAIFGSLFQSIMDKAARRNLGAHYTSEENILKLIKPLFLDKLREEFEK
                     IKHNINRLLEFHKKLRQLNFLDPACGCGNFLVIAYRELRLLELDILRASKIYQSELSI
                     HRLINLNVDQFSGIEIEEFPAQIAQVALWLMDHQMNLLVSEEFGLYYARIPLETSAKI
                     VCGNALTIDWEEVSPARNLSYIMGNPPFVGAKFMDDEQRKDVAAVFGNIKNAGLLDYV
                     AAWYVKAAKMIKGTTICCAFVSTNSITQGEQVGALWNWMLQEGMKIFFAHRTFQWNNE
                     ARGKAAVHCVIIGFADFDITPKWLFEYEHIKGEPQKTKAQNINPYLVDAPDIVLSNRS
                     LPICPVPSIGIGNKPIDGGNYLFTEEEKIEFLKKEPQAEQYFRRWLGADEFLYGYHRY
                     CLWLGDCSPEQLKKMPEAMKRIDAVRNFRLASKSAPTRKIADTPTRFHVENMPSDEYI
                     LISRVSSERRLYLPIGYIEAQNLTSDTALILIGAKLYHFGILTSIMHNAWMRTVCGRL
                     ESRYRYSAGIVYNNFPWPENPTEKQKQAIETAAQGVLDARAQFPNSTLADLYDPLTMP
                     PVLLKAHQTLDRAVDTAYGKTNFTTEAQRVAFLFELYQKYTSLFAADKPKRRAKVVKI
                     PL"
     gene            5336..5794
                     /locus_tag="PLF77_03555"
     CDS             5336..5794
                     /locus_tag="PLF77_03555"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HPC07963.1"
                     /translation="MRIVMLALCCFICLLTSAHALEMYKCLDNNGNAVITSTPQDGMT
                     DCVLMDSSEDSVPEDNANDNGSETKESVAGSGNANQQADEKLRRQCANLKVYREEERT
                     YCEGVPQSYKSGNEEMKKKAQDRMASSNASASQLCDYYRGMVRELEAKCP"
     gene            complement(5844..6632)
                     /locus_tag="PLF77_03560"
     CDS             complement(5844..6632)
                     /locus_tag="PLF77_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HPC07964.1"
                     /translation="MTDKKNDDIRIAVLENYRKVAVSDQSGCGCSSASCCARPDNAAS
                     LALGYSANDLSVVPEGADMGLGCGNPQAIASLKAGETVLDLGSGGGFDCFLAARSVGD
                     QGLVIGVDMTPEMIAKARRNAEQGGYANVDFRLGELEHLPVANGIVDVIISNCVINLS
                     PEKEKVFREAFRVLKPGGRLAISDIVATAPIPDEIKKDLALYACCIAGASSVRDIENI
                     MTETGFEQIKIILKEESRIFIRTWLPDSHAEDYVVSATIEAIKP"
     gene            complement(6922..7566)
                     /locus_tag="PLF77_03565"
     CDS             complement(6922..7566)
                     /locus_tag="PLF77_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07965.1"
                     /translation="MLFVGRQSEKKIIMEALNCGRNIILGGKFGIGRTRLIREIAKLV
                     NERKFIFVDFSQTPGKMSEKIMKALGIPRRIKNQTQQMGYKSMRYRIAHANTIKQHRP
                     VIVFDNMAKITAQKKILLRHLILEQHFQFIAVVENFLPQKNLFDLKAQLMPATTLNLR
                     HLKKQEVENLLRTYAKKHHVAWTENDIRNPACLSDGYPLSLKEMLKKKIIPEDR"
     gene            complement(7601..9256)
                     /locus_tag="PLF77_03570"
     CDS             complement(7601..9256)
                     /locus_tag="PLF77_03570"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HPC07966.1"
                     /translation="MGNVLPQKSVSDMRRHLKIIADGCSRFRMLSLNRQLEAAQLLLD
                     ENPPIDIAVLGQFKAGKSSFLNSLLGQNVLSVGAIPVTTAVTRLQYGTKAKALVRHFD
                     GNITEIPLADIVKFTSEAENPGNAKNVSIVDIELPSLKKYSGLRLVDTPGLGSVYKYH
                     QSASEEWLPSVGTALLAVSADRPLSEHDLELIRELIAHTPNIILLLTKVDLLSSDQQK
                     EVVAFFQATLNRELHRTLPVYLYSTRDNTDEYKQVIEENIFKTICADRDTEFLRILNH
                     KVRSLSQGCLSYLNIALNSSLQADMNRENLRNRILNEKVNEDIVREELGIISREHQRQ
                     TRALIEAYLEKFRVPLTKKVMRQLVDELPSWKGNLWKLSRKYEVWVSETLSEEMRIIS
                     KNEHRNFLGTMKKAHAAISRSLDAFCNFLGDNIENVLGVKMTEVQWKIDAAEPDHPDI
                     SFTKTFDIHLDLIWFLIPMMFFRKIFERHFIDGIPKEVEINLSRLAYQWEKSVNHAID
                     EMRLQAFNYIHEELATIEALISGTKGQTEDIRGLIDQIEKITI"
     regulatory      complement(9281..9345)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(9948..10655)
                     /locus_tag="PLF77_03575"
     CDS             complement(9948..10655)
                     /locus_tag="PLF77_03575"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPC07967.1"
                     /translation="MMVQKDKLSKILNKQNQLPYQIAEYLAEKIIHMQIKPGERILEA
                     KIAQELGVSRSPVREALHILNEKFLVELLPRRGAVVAGFSIKFIEDLYDILKVLYLLL
                     SQKGVSKISDENFNQIAPFFQTMEECANREDFEGYYNAIFDFMEAAIKIVDNPILERL
                     IMNLWPTKKRMMYATLFKRKHDLKSNLDFFLKSVAPYLEKRIDLVENNIVDYLEQEKE
                     NAILLAKEFELSNTTKK"
     gene            11092..11622
                     /locus_tag="PLF77_03580"
     CDS             11092..11622
                     /locus_tag="PLF77_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07968.1"
                     /translation="MRSKRMCKIVPPEKWLKTQITRLCLYFLGMGIETAYKNVPQAKR
                     EMDTLPDQFSFCFNVVNGPSMMILKNGHTVRYVGEKEAYHADLELVLKNIEFAYLMMT
                     GRISTPDAVYHNRQFVRGNLAYMMTMIRVLNIAQTLLFPNFLLRFYIKTVPRLTMKTM
                     VNRAKAYANATIGLFA"
     gene            11655..12860
                     /locus_tag="PLF77_03585"
     CDS             11655..12860
                     /locus_tag="PLF77_03585"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPC07969.1"
                     /translation="MQKKEECPKYYEFFNSVKIISGYKALDNIASELDMLGARKPIII
                     TDKGVSQAGLIDHVVNSFGGTQVAIGALYDDTPVDSSIVAVNEIAGIYRQQECDAIIA
                     VGGGSVIDTAKGVNIVISEEADDLMKFTGNNRLTKPMKPFIVVPTTAGTGSEVTMAAV
                     IANPARNVKMQFTSPLILPKVAILDPVMTMSMPPKITAATAMDALTHAVEAYIGPQKN
                     PVSDAFAFSAIELIKANIIDAAKNGKDRDVRLAMANASLLAGISFSNSMVGIVHALAH
                     ATGGLCHVPHGVANAILLPHGLEFYIKKSPDIAARIGKLLRFFMEPEDYAKIPVNERE
                     KNTVAAIRDMNDSLNKICSLPLTLSQAGVTKDKLEAIANAAINDGAIAFCPVEASYED
                     ALEILKAAF"
     gene            12914..14548
                     /locus_tag="PLF77_03590"
     CDS             12914..14548
                     /locus_tag="PLF77_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HPC07970.1"
                     /translation="MNAQVSGTAEHQASAAKDQGVKNDVTVCTNPASCEVIAIYPIHT
                     LEDVKNAVTNARRAQPAWQALPLKKKIAYVKKFAEYIQKNSAKLAEIISQDNGKTIFD
                     AMFAEVGPATLATSYYCKNAAEFLADRKLKAGNIMLMNKRSRIVRVPYGVVGIISPWN
                     YPFAIPFSEVVMGLLAGNTVILKAASETQMVGHALKECIDYAGLPEHVFTYINMPGNK
                     AGDALLDAGVDKLFFTGSVPIGKYLMKKASENLTPLVLELGGNDAMIVCEDADLYRAA
                     CGAVWAGMQNAGQSCGGVERIYVDRKIYHEFLAVLKERVEGLRVGDGNCLMTDIGAMT
                     TRKQMRTVQEHIDDALAKGAKIFAQSKCPQNCKGNFIPCTVLTDVDHTMLVMKDETFG
                     PVVGVMPVENMEQAIALANDSNLGLTCSVWSKNRKKAVQIGRRVMAGAVMINDHLMSH
                     GLAETPWGGFKESGIGRTHGDIGFAEMTQPQVIVDDILSAMPFVRRNFWWQPFEKLQY
                     DGMKGLIDLFHGKGFWERFVGLNKLGHAFYRSFVKK"
     gene            14791..17493
                     /gene="fdhF"
                     /locus_tag="PLF77_03595"
     CDS             14791..17493
                     /gene="fdhF"
                     /locus_tag="PLF77_03595"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014956451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:15853..15855,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPC07971.1"
                     /translation="MSETFLLNGKETPFEQGQTIVEAAKNGGVEIPTMCYLKGSTPTG
                     ACRICCVEVKGARSLVAACSTPVTPNMEVFTETELVHKSRKMVVEFMLTSGKHNCMTC
                     EANGNCRLQDLAYFYKITTPRFEESEQLYPTEDENPFIVRDFSRCILCGRCVQACNEV
                     VVNRAISQGYRGRKSKIVTGGDLPYNHDDSECVFCGQCVQVCPVGALTEKKAKGLGRP
                     WEVQKIRTTCPYCGVGCQQLLHVKDGKIVKVTAVEDAQPNQGRLCVKGRFAYDFIYSG
                     ERLKTPLIREKNGEFREASWDEALDLVANKFKEIIKKHGPDAVAGVSCARSINEDSYQ
                     MQKLFRAVFGTNNIDHCARTUHAPTVAGLATSFGSGAMTNSFADYAKAKMIILVGTNI
                     SEAHPVASTFVKDAVLAGAKLIVIDPRRTDMVDFADVHIPLKVGSDVALINALMNVLI
                     TENLYDKKYVESCTEGFEELKKKVMEYTPEKAAKISGIPADTIRELARTMASIKPAML
                     MYTLGITEHICGVNNVMSCANLQMLMGNVGVELGGVNPQRGQNNVQGACDMGALPNVF
                     PGYQQVANAEARAKFEKAWNVKSLPDKPGMMMPTMMKNLTTGKVKGFYVFGENLANTE
                     PDIKHVEQELQSAEFLVCQDIFPTETTRFAHVILPAAAWSENDGTFASSERRVNRVRT
                     ASVPPGEAKPNWWIFKEIAKRFGHEWASNSAQEIWDNEVSVLAPSMAGIKYARLEGDG
                     LQWPCPTADHPGTPTLHKGGKFTRGLGMLKAIDYIPPAEVPDKEYPMVLSTGRRLYHY
                     HTRTQTGRSEGINDLLGEETADISPADAEAKGIKNGEKILVKSRRGKVEVKARVTEEV
                     PQGLVWMAFHFREACANWLTNPVYDPVTQTAEYKACAVKIEKLK"
     gene            17691..18464
                     /gene="modA"
                     /locus_tag="PLF77_03600"
     CDS             17691..18464
                     /gene="modA"
                     /locus_tag="PLF77_03600"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPC07972.1"
                     /translation="MKKSNFKQVITKILLTVIGVAFFAGLSGQAFAEEKISVAVAANF
                     ISAFKDMGADFEAKTKIKVEGTFSSSGNLYSQITNGAPYDLLLSADEERPAKLNKDGL
                     ADNPFIYARGQAILWSANKEFCKAKTWQDALKNEQIKKLAIANTQTAPYGTAAQKALE
                     KAGMWTDVQSKLVVAQSIAQSFQYASTESVDAGFCALSATASPEGKKGCFYVLNEAPE
                     IIQSACILKRTTNRAAAEKFAEYLRSPAAQEIKVKYGYR"
     gene            18465..19124
                     /gene="modB"
                     /locus_tag="PLF77_03605"
     CDS             18465..19124
                     /gene="modB"
                     /locus_tag="PLF77_03605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HPC07973.1"
                     /translation="MDYLAIYVTLKLALVTTVVLMVIAAPVAYALAYNRFSGKSFLEA
                     LIYLPMALPPTVIGFYLIILMGPKGFVGKMWQMLTGGSLLFTFIGITMASIIYSIPFA
                     VQPMKAAFSKIDRRLLESAYVLGLSKKATFFRVIIPNSVSGIAAAAILVFLHSIGAFG
                     VLLMVGGSIPGETKVASIAIYEAVEMMNYKAAGLIALSFIPISYAFLLLVNKLNEGAR
                     S"
     gene            19121..19834
                     /locus_tag="PLF77_03610"
     CDS             19121..19834
                     /locus_tag="PLF77_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC07974.1"
                     /translation="MSIEVSFKKKLKYFDVDISFSCAKKNMVVMIGPSGGGKTTIIRM
                     LAGLEKPKEGKVVFGDEVWFDSLRRIDVPPQKRRLGYVFQDYNLFPHLNLFDNAAFAA
                     VDKKEVEDLFGIFRIGHLKKRKPHMVSGGERQRCAICQALARHPRILLLDEPFSALDA
                     ITRRGLREELKNLKGKMSFPIIYVTHDITEALYLADELLPVVDGKIDDQWLQRMMTRE
                     PSSEVALRAAREPRLTLVY"
     gene            20041..20622
                     /locus_tag="PLF77_03615"
     CDS             20041..20622
                     /locus_tag="PLF77_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit E
                     family protein"
                     /protein_id="HPC07975.1"
                     /translation="MNICTYSYEEYIHLVKSFHGTLAPGLLIGGFIVDLAMKNLPEGE
                     FFDAVCETPVCLPDAVQILTPCTIGNGWLSIVNFGRFAVTLYEKYSGRGVRVYLDTEK
                     LNNWPEVRDWYLKKKKKDQQDAEVLFAQIKEAGHQLLSVQKVQVEPEKVRRKKMGPVG
                     ICPACGEAYPLKDGGKCMNCQGATPYSSVVPVK"
     gene            20645..21418
                     /locus_tag="PLF77_03620"
     CDS             20645..21418
                     /locus_tag="PLF77_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HPC07976.1"
                     /translation="MLKTGITLSRLFEKTEPAARNRQIADLRIGLSYVGIKLDNGVAG
                     LAAVLPGSTARGCTVLKEAGTYIGSPAVETLQYLVEAKDALRRAIGVATANALVLPES
                     GKTEDREATTYLNLQPGEKVAMVGLFAPLVERIRSTGATLTVVEKNPERLEVLSAEEK
                     QKALKDCDVAIITATTLLNGTFEETIGLLGRPRSVVLMGPSTPLVPEIFHDTPVTQLG
                     GSIVADAAKALQVISEGGGTPALRHYLRFVNLLLPDKSR"
     gene            complement(21423..22214)
                     /locus_tag="PLF77_03625"
     CDS             complement(21423..22214)
                     /locus_tag="PLF77_03625"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPC07977.1"
                     /translation="MNLFDKDIQISESDHSRFAGTISENWSVNGNPNGGYLMAMIVNA
                     MLRKSDKKATPVVTANYLSRCVPGKIEIHIAEITRSRQFTRFEARLIQEGDEKIRALG
                     TFVSDNNDCAITRYESKAPELAPIEECVPMSVPPSYTLFQNLDLRLDPACAGWMTGGE
                     LTDVSLNKGYFRFRDGRPLDLFSLFLIIDSMPPAAMASQGATAWVPTIELSVNVRNLP
                     KTDRLKCSLRTRFITCGILEADGEVWDEKGNLAAISRQIAQFKKI"
     gene            complement(22295..23305)
                     /locus_tag="PLF77_03630"
     CDS             complement(22295..23305)
                     /locus_tag="PLF77_03630"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPC07978.1"
                     /translation="MPDTLKQYAEKLGFPGSETMAKIFAILYDNADSVAVAEALPGSV
                     ADIAARTGLNEERVRQIAQKLLRKGAISRVLAKGDYYRLYPAMIELRDAVVIMPDCPE
                     ELFELWEIIIKEEMPRIVPVFNKLNLAPMLRVIPIEETVESSSRVLDADSARSIIRSA
                     RIITAIPCPCRTQAKHLGKTKDCIAPDGVNLCMQINGFAEAAIDRGVGEVLSTDEALR
                     RLAVAEEAGLVHTVRNNVKEDMFMCNCCPCCCTAMFLHNQAGFDASFAPSRFLAQVDA
                     DTCTGCGACEEKCAFNAITVGDTAVIDCNKCMGCGNCVIVCPVEAIALQEARPPEFIR
                     RT"
     gene            complement(23411..25645)
                     /locus_tag="PLF77_03635"
     CDS             complement(23411..25645)
                     /locus_tag="PLF77_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HPC07979.1"
                     /translation="MGALHKTGCVLCAQNCGLEVDVDNNRIVKVKPDKSNPKSEGYFC
                     RKGMNVAFHQHNADRLKYPLKKVGNHFERITWDQAIDEIAAKLKDIVGQYGPRSFAYM
                     GGGNQSCHLEVAFGVSLMRTLGSQYQYSALAQEFSGAFWVCGRTHGKQYLHDGPDVKN
                     TDVLLIFGWNGMESHQIPQAPRQLQRLSKDPDKLLIVVDPRISETAKIADIHLRIRPG
                     TDALLLKSMIAIILKEGWINKDYMENHTAGFEQVKSLFENFDVRAALKVCELDFDQVY
                     KVTQAYAKRKSSLRYDLGIFMGRHSGLNSYLIVMLQSICGRLCVPGGNIIPGHIVPFG
                     PNSDERNQKTWRTVATNAFPVCGCFPPNVMPEEILSDHPERLRAVLVSGLNPLRSYAD
                     TTAYEKAFQRLDLLVTVELAMTETAALSHYVLPSRSGYESWDGTFFPFTYPEIFFQMR
                     RPIIAPEGEPLEQGEIDLRLADKLGLIPPIPEKLYKTAEESHAAFRQALMEYAMTEPK
                     AMKTMPFILGKTLGKAMGSVHLAALWGMLQVAPQSFQASAARAGFTPGPTLGEEIFRK
                     ILDHPEGLWVGKTDEGNNFAEIKTPDGKINLFIPELLDELKSIDAQREEAALVMPAEF
                     PLVLMAGRHTSMNANTLMRNPAWNEGKRPCTLAMHPDDAAVLNLKDGQPVRVTTEAGS
                     EEIELEITETVRPGHVTMPHGFGLIYNGVKYGANVNRLTKTTNRDQFGTPMHRYVRCR
                     VEAA"
     gene            complement(25696..26118)
                     /locus_tag="PLF77_03640"
     CDS             complement(25696..26118)
                     /locus_tag="PLF77_03640"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HPC07980.1"
                     /translation="MQEKDCIFFQLAKANQLASRFLSQKVSELNLTPVQALVLGFLHQ
                     EDQITSAELGKKTGLDSATLTGILDRLEAAEFIERKNNPEDRRSIHIHLTLKGNALSK
                     EAIRVIAGANLEFLQSLTKEEKRHLFDIIKKLRLGAAR"
     gene            26642..29752
                     /gene="fdnG"
                     /locus_tag="PLF77_03645"
     CDS             26642..29752
                     /gene="fdnG"
                     /locus_tag="PLF77_03645"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012612356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:27197..27199,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HPC07981.1"
                     /translation="MNVSRRGFLKISGAVAAVSGLGISLKSIAAHARELKIMHAKETT
                     TVCPYCSVGCSIIVSVRDGKVINTEGDPDSPINRGALCSKGGSIYQMAVNENRLGKPL
                     YRAPYAREWKEVEWEWAIDKIAQNVKKSRDASFKKVNAKGEVVNRTEGIASVGSAAMD
                     LEECFTYQKFLRGLGLVYIEHQARIUHSPTVPALAESFGRGAMTNHWVDFKNADVILI
                     MGSNPASNHPVSWKWIQEAVDKRGAKIICVDPRFTQSAAKAHLYAPLRSGTDIAFLGG
                     MIKYILDNKLYFAEYVKNYTNASFLVNPDLKLPGENNGVFSGLTDGKYDKGTWSYQKG
                     PDDVIKKDPMLSDPHCVFQLLKKHYSRYTPDVVSRITGTPKDKLLEVYKLYASTGKPN
                     KAGVDLYAMGWTQHTVGTQNIRTMCMIQLLLGNIGIAGGGVAAMRGESNVQGSTDHGL
                     LFHIWPGYLGTPTGSLATLKDFIEKRTPKTKEKDSLNWWKNTPKYVAGFLRSMYGSDM
                     TLEDAYEALPKLDDGANYSWLTIFDQMYKGKFTGFFAWGMNPACSGAHSNKVRQALGK
                     VDWMVNVNLFDNETGSFWRGPGMDPSKIKTEVFMLPCASSVEKEGSIANSGRLQQWRY
                     KAIPYYGDSKPDGDIMSEIFFKVKKLYEKQGGPNAAAITKMTWPYGKHEGKEFHYEPR
                     KVAAEINGFFLEDKTVENPTKKGEFKSFKKGDLVPTFAWLQDDGSTSSGCWIYCGSVS
                     EKGILPMRRGQADPTGLGLYSEWAWSWPVNRRIIYNGASVDLNGKPWDPSRAVITWDA
                     KEEKWKGDVPDGAGNPGKGRPPFIMKPDGVASLYGPGLADGPFPEHYEPLECPVEKNL
                     LSPQKNNPAIKRFDKEGVGSDMDVYSGVGTCDPRFPFICSTYRVSEHWQTGVLTRWCP
                     WLAEMQPGMFVEMSQELAKMRGIETGDKCTVSTARGKVVCTAIVTPRFKPFDIDGNEI
                     HEIGIPWHFGWITTKDRPFNKGDKVAEVFTTGDAANLLTPTIGDANTMIPESKAFMAN
                     VVKGVK"
     gene            29754..30557
                     /locus_tag="PLF77_03650"
     CDS             29754..30557
                     /locus_tag="PLF77_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPC07982.1"
                     /translation="MTEKIKLYDASKCTACRGCQLACKQWNGLKAGQTEHTGTYQNPP
                     SLQHNTYMIIHFQDYVAPEGDVRWIFRKEACMHCTNAACVTVCPSGALYYNKEFGTVG
                     MDREKCIGCKECISACPFEVPQYDKKTEKVFKCDMCETRITNNLTPACVKACPTGALK
                     WLDKEEAVKTAEARVQQLGGDANVYGDKFVGGTHMMYVLKEKPEVYEGIKQDPKVPFS
                     LTVWKSWLKPLSLLGAGGVIGGALLHYILHGPHKPHPEHYDQDTKQGGA"
     gene            30559..31206
                     /locus_tag="PLF77_03655"
     CDS             30559..31206
                     /locus_tag="PLF77_03655"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HPC07983.1"
                     /translation="MKSKMVMVTSKYERFVHWLLAISCLLLCYTGLGIMYKELNFLGF
                     IFGGLKGLATVHDIMAVVFAISLVLAIAMWWKEAGLFVFPEDWEWIKAAGGYLWHLDH
                     VPETGKYNPGQKAFFLTVALFGLLMICTGFFMWYPHVLPAALMDWVYVLHVLGFVVIF
                     AFFFVHLYLGTIGNPGSVSAMISGKMELPVLRMLHPKWVKEMEHEGKLMIADDKK"
     gene            complement(31316..33013)
                     /locus_tag="PLF77_03660"
     CDS             complement(31316..33013)
                     /locus_tag="PLF77_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPC07984.1"
                     /translation="MKFLPAVVSSFVESKIVKANIRLLVKYLIAIIALITTYSILFHY
                     IMAMEGQEHSWITGFYWTMVVMSTLGFGDITFTSDIGRLFSIIVLLSGVILLLILLPF
                     VFIKFFFAPWMEAQTRNRAPRELPVDTRNHVIITNFDPVTEALIEKLKSYNKNYVVIV
                     EDLAKALELYDRDIRVALGNIDDPETYNKMRVQNAELIVASNSDQMNTNITLTLREDL
                     EHIPIITTAESPHSVDILQLAGSSRVLQLYDIMGESLASWTVAGECKANIIGRHKELI
                     IAQTPVIGTPLVGQTLAESKLRETFGLTVVGIWERGKFIIPKSDYKINRTSVLVLAGL
                     ESSLVQYDDVYSFYHICKIAGDPVIIVGGGRVGRAVANHFQERDIPYLIIEKSRRKNE
                     DDSHFVYGDAADIHTLEKAWFEKAPAALITSHDDATNIYLTKYLRSLRPDMQILSRTS
                     EDRNISTLHRAGADFVMSYASLGANTIFNFLMNEETLLLAEGLNFFHLNTPRSLWGKT
                     LADSGIRERTGCTVVALSRKGETHINPEPDIRLGKEDELILIGTYETERAFLNEFPV"
     gene            complement(33157..34695)
                     /locus_tag="PLF77_03665"
     CDS             complement(33157..34695)
                     /locus_tag="PLF77_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07985.1"
                     /translation="MHTQNTTVQNRFWGETTAFLALFLDNVGTLIFFSAILVFTFNYP
                     ADMILTRMIPGTAVGIFIGDIVYTWLALRLRKKTGRTDVTAMPLGIDTPSTIGIAYAV
                     MGPAYIATRDAQLTWHIGMATLFMIGAVKIVTSFFGGWIQRVIPTAGLLGPLAGIGLL
                     LLGFLPLVEIFNEAIVGMVALGLIFTTLMSKMQLPGRMPGVLAAVILGTGIHFFLGFS
                     GYLPEFAAPTLDMHFALPVPSVEFLKTLPQSVHYLTIAIPFGILTIIGGINNTESARL
                     AGDDYRTRDILLTEALSSLIASFFGGVAQTTPYIGHPAYKKMGATWWYTLLTGILIGV
                     CSIIGLLSVFVSLIPRAVIAPIFIFIGFEIMRQAYKDSPAAHSPAVSLSILPVIASMV
                     LIVLGQFTGASGIILEKLPGRLLIMHQTLTMLSNGFIITGLLWGSMLAFLIDHQAKRA
                     ALCAAVSAILTLFGIIHSVLPTGELYLPWHIQSQAHYMTALAYFTLSAILLLFNPPKA
                     EAQT"
     gene            complement(34946..35701)
                     /locus_tag="PLF77_03670"
     CDS             complement(34946..35701)
                     /locus_tag="PLF77_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPC07986.1"
                     /translation="MEELQSHERVMNILVRQIFSRQLKPGDKLPSERQLSKDMDVDRA
                     SLRIALKHLELMNVLTIRQGDGVYVRDYMKGASIDFLRMFFLQTEEPDNEWVADPYIL
                     DEIWEFWTFFLPEMLRLAIPKITPRDIKTLIDIFDEELANLHDKKHLIDLELLSQEAI
                     AEAGNNIVVILITHTCRPLRKKMIEMFYDSLDEERIKKHIEIKKELVLSYLASATPDS
                     LALIERYRETLNGYRATLRKLLFEQEKASRRKK"
     gene            35927..36964
                     /locus_tag="PLF77_03675"
     CDS             35927..36964
                     /locus_tag="PLF77_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPC07987.1"
                     /translation="MKMDYKYLKIQKENHSLWVEMKNPPVNFLTVAMLEELFDLVKQV
                     SRDPSIRVFILTGGIEDIYIMHFSIPELFTLSKDNKKIRLNLFIKTRLTAAILKHFTT
                     CTNGLMDGFGWYEKLVLKFAKVLSGYSSSLYLWFIMQRLYMAIERLNKITIAAINGNC
                     NGGGTELSACFDFRFAVGDQGFTIGQPEVLINIVPGGGSTQRLPRLIGKAKALELMLR
                     GNQWDAEEAHRVGLLTGFFNKAEFKTKVQEFADLMSKRPMTAVDAIKKCIHNGMETTI
                     RHGLSIEMEQSIRCLDTDDTMNAMQAYIRYLDQHINAMDPQKMTTEEINQVVQDTVKH
                     MEEGKLYKFKH"
     gene            36979..37890
                     /locus_tag="PLF77_03680"
     CDS             36979..37890
                     /locus_tag="PLF77_03680"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC07988.1"
                     /translation="MKKTTMGANSKINSSANAILVTGASSGIGWATSLELAQKGWRVF
                     AAVRKEADAKKLRDASSNKVTPVIMDIVDYDSVKRGASEIEKALGGAGLDALFNNAGI
                     SVQGPLEILPIELFEQQIRVNVFGHIFVTQTFLPLLRKAQGRIVFTSSESGRVTLPLM
                     APYSSSKFALEAVASALRIELRPWKIRVSSVELQTIKTPMWEKIDATTEKLFASVPKQ
                     ARDLYKDELRTLSVFPKWQAEMGISMKKAVRVIIRALSVKRPKARYLVGWEARFLVYC
                     HNVTPIWLMDWFSSKIMILLGKFIKPK"
     gene            38016..39425
                     /locus_tag="PLF77_03685"
     CDS             38016..39425
                     /locus_tag="PLF77_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPC07989.1"
                     /translation="MRDVYIIGVDTIKFGKHMDKSIKDLAITTATGCLKDAGLDKKDI
                     QALWFANAGWGEGGQMTIRGQVALKGMGIEGIPITNVENACAGGSTALHHAWYGVAGG
                     LYDITMAVGAEKLYFSNKNLVFFGFLGGIDIENVLNIAKTLSVFTLTPEEKVKVEEFK
                     QRYQAVKKAANNGGVKKAKAKESKWKAYRDKFTAFIVLGEKIGYDTVRQLARMSSGDH
                     SPFMDVYGFAARQHMKKYGSTAEQMAMIASKNHFNSTLNPNAQYRFDVPVEKVLSDRI
                     VSWPLTRSMCAPIGDGAASAILCSEDMVKKLGLTHQAVKIRASILGSAIRTSFDSDIP
                     EIGERLSKQAYEKAGVGPQDINVAEVHDASAFGEVVQAENLGFCPKGEGGIFAEKGET
                     SLKGRIPINPSGGLISRGHPIGASGLAQVHELVTQLRGNAGKRQVNNARLALAENGGG
                     ALGTEEAAMCIHILEAPSS"
BASE COUNT        10206 a         9318 c        10068 g         9849 t
ORIGIN      
        1 tctctttttc gccaaccttg attctttcgc gataaaatat tttccctgcc atcattctac
       61 ctccctattt tttctttttg tcagggtttc gtttctctcc caggctttcc cacaggcccg
      121 cgcctgccgg tttaaccgga agcacaacgt catgccccca gagcctgggt cttaaccagc
      181 cttttgtatt caccatgtct tccggacggg ggcaacacgc atcccggtcc agagaaatca
      241 cccgaatatt tttttgcatc agcgccgcaa caattacctc gtcgggaaaa tctctcaacc
      301 catcatactc gataaaacgt ccgctccatc ccgaagcggt gagaccgaca gccgccgcgg
      361 ctccgatcat gccgccgttg gttccgccgt gaccggaaag atgagcgctg cccgccgcct
      421 ttacggcgtc cttcagggta acgactcttg ctgtgcactg ccttccaaat tgctccagcg
      481 caggcagcgc cagatcgcct tcgacaacca cggaaaggcc gggatcgctg ccgggaagcg
      541 acatcgcttc gatgtgttta atggccgcgc tttttaaagc atccacatac gatgaatccg
      601 ggcagtccac aaccacacag gctgaactgt tgtgcgacgt ataaggaatg gccggatcta
      661 caagcaactg ctggcggaca acaccccaga ttttgcattg agcaggcagg actttttcaa
      721 aacggcgggc gacctttccc gttccgtaat ccgcctccac aatgtccgta tcgtcaaaac
      781 caatataaac gcgcatcttt gccatcatgt taaaaaaaac atccttaacc gattatagag
      841 tcaccgtttt agcctgtcaa tgaattaaaa atgtaaacat cgaaattctc aaatgatttg
      901 tgataatata tactttgacg caatccatta tttcctgtat attactaaaa cgattcgaaa
      961 tgaattaaat gaagatcata gatgattgcc aaggaggatt gaaatgaaaa cggatgaata
     1021 caaaaaaata atcatgtttg cagttgacaa tgaaattgaa gcctacgagt tttacaaggc
     1081 ggttgccgat aaaacaaaag atggcggttt gaagaaaatc tttacggaac tggcgcagga
     1141 agaagccggt catcgcaaac ttctggaagg cttggcatcc ggaaagggcc agatgcattt
     1201 tgacgaatcc aaagattaca aagtggccga atcaatcaat aagcccaaac tgagtatgag
     1261 catgaaaccg gcggatgcca tcgcgctggc ggtcaagaat gaacaggagg ccatggaaat
     1321 gtacaaggaa atggccggat gcagcaccga tgccgagcag aaaaagatgt tccagtcgct
     1381 ggcgaatatg gaacaggggc acaaagtcaa actggaagac acctttacca acatggcgtt
     1441 tccggaagcc tggtaaattt gattgaataa atatttaagg cgggggaaaa cgtttcatta
     1501 tcccccgcct ttttctttat ggtcattcct tggattgtaa attaaaaacc cacctgatgt
     1561 taattgttga aaattgctta ttaataaaaa tattatagtt tacagaatct tgcaacatta
     1621 cctgttaaat gttggcggct actttcaagt actattttat aaatgaaaca tttattttga
     1681 tcattctaga acgttttgta aaaggcggaa ggttaaacaa gcaacaagca taaaaatatt
     1741 cttgaaatgt aatcaataag ttgtattatc tccagcaatt aatcaaataa atatttctta
     1801 agcagacggg caattattta tattgctaaa tatataaaaa cttatataat gggattgcga
     1861 tgacgaaatc gttaaaattt atcggttcaa gtctggatga tcttcgtgat tttccggatg
     1921 aagcgcgtcg agcagcaggt tttgaacttc gcgcacttca aaatggactg gaaccacgcg
     1981 attggaaacc cattgtgtct gttggaccgg gcgtaaaaga gatccgcatt catgttcttg
     2041 gtgaatggcg ggtgatgtac gttgccaata tccgggatgc catttacgtg ttgcatgcgt
     2101 ttcaaaagaa aacgcaaaaa accggtctgc gtgatattga attagcgcgt aaaagataca
     2161 agcaaattgg agagtgattt atgaaaaaat ccattgtaac ttcatcggac aacgtattca
     2221 ttgatttggg atattccaaa gatgaagctg cgattatgca aatgcgggcc gatttgatgg
     2281 ccgatttgcg cagatttatc aagaacaaaa aacttactca gactgaagct gccaaaattt
     2341 tcggcgtcag ccagtcgcgg gtttccgatt tgattaccgg caaatgggaa aagttcagtc
     2401 ttgaaatgct gatcatcctt gtaaccaagg ccggaatgaa tgttaaactg aaaacggccg
     2461 cgtagtcgcg cagtcatgat tcaagattcg caaatacttc tcacgctgat gttaaaaaaa
     2521 gaaaggattc gatgccgcta agctggaacg aaattaagac acgcgcgctc gctttttcca
     2581 aagaatggca ggaagaaacc tccgaggacg ccgaggccaa atccttctgg gacggctttt
     2641 tcaacatctt cggcatttcc cgcaaacgaa cagccagttt tgaagaaccg gtcaaaaagc
     2701 tgggcgaaaa gtacggctac atcgatttat tctggaaggg cgtgttaatc gtcgaacaca
     2761 aatcgcgcgg caagagtctc gacaaggcct actcacaggc gctggattat ttctccggca
     2821 ttgccgagcg cgatttgccc aagtatgtca tcgtttccga cttcgcccgc atccgccttt
     2881 acgatctgga aaaacacgaa cagcacgaat tcgacctgaa agacctgcac aagaacgtgc
     2941 gcctcttcgg tttcgttgcc ggataccaga cccacaaaat tcaggagcag gaccccgtca
     3001 acatcaaagc cgccgagcag atgggcaaac tgcacgaccg gctgaaggaa tccggctaca
     3061 caggccattc gctcgaactc tacatggtgc ggctgctgtt ctgcctgttt gccgaagaca
     3121 cgggtatttt tgaaaagcag caattcaagg aatatgttga acagagaacc tctgaagacg
     3181 gctccgacct tggccaccat ataagcacct tgtttcaggt gctgaacacg ccaccggaca
     3241 agcggcagaa aacgcttgac gagcagcttg ccgcttttgc gtatatcaat ggcaaactct
     3301 ttgaagaaat gctgcccaat gccgccttcg attccgccat gcggcaggaa ctgctcaact
     3361 gctgcgcgct ggactggagc cggatttcac ccgccatttt cggttcactc ttccagtcca
     3421 ttatggacaa ggccgcgcgg cgcaacctcg gcgcgcatta caccagcgag gaaaacattc
     3481 tcaaactcat caaaccgctt tttctggata aactccggga agagtttgaa aaaatcaaac
     3541 acaacataaa ccggctgctg gaatttcaca agaaattaag gcaactgaat tttctggacc
     3601 ccgcctgcgg ctgcggcaac tttctggtga ttgcctaccg cgaactcagg ctgctggagc
     3661 tggatattct gcgtgcgtca aagatttacc agagcgaact gagcattcac cggctgatta
     3721 atctcaatgt tgaccagttt tccggcatcg agattgagga atttcccgcg cagattgcgc
     3781 aggtggcgct gtggctgatg gatcaccaga tgaacctgct ggtttccgaa gagttcggcc
     3841 tctactatgc gcgcattccg ctggagacca gcgccaagat tgtctgcggc aacgcgctga
     3901 ccatcgactg ggaagaagtt tctcccgccc ggaatctcag ttacatcatg ggcaaccctc
     3961 cgtttgtggg agcgaagttc atggacgatg agcagcgcaa ggatgttgcc gctgttttcg
     4021 gcaatataaa aaatgccggt cttctggatt atgtggccgc ctggtatgtg aaggccgcga
     4081 agatgatcaa aggaacaacg atttgctgtg cctttgtttc caccaattcc attacgcagg
     4141 gcgaacaggt cggcgctctt tggaactgga tgctgcaaga aggcatgaaa atattttttg
     4201 cccaccggac gttccagtgg aacaacgaag cgcgcggcaa ggcggcggtg cattgcgtga
     4261 ttatcggctt tgccgatttc gatattaccc ctaaatggtt atttgaatat gaacatatca
     4321 aaggcgagcc gcagaaaact aaagcgcaaa acataaatcc atatctggtt gatgcgccgg
     4381 atatcgtttt aagcaatcga agtctgccga tttgtccggt tccatctatc ggcataggca
     4441 ataaaccgat tgacggcggc aactatctgt ttacagaaga agagaaaata gaatttctaa
     4501 aaaaagaacc acaggcagag cagtatttcc gccgctggct gggagcggat gaatttttgt
     4561 atggttatca tcgttattgt ctttggctgg gtgattgttc gccggagcaa ttaaagaaaa
     4621 tgccggaggc aatgaagaga atagacgctg taagaaattt tcgattggca agcaaaagcg
     4681 ctcccacaag aaagattgct gatacaccaa cccggttcca tgtcgagaat atgccaagtg
     4741 atgaatacat tttaatttcc agggtttctt ctgaacgtag actttaccta ccgattggtt
     4801 atattgaagc gcaaaatctg acaagtgata ccgcgcttat tcttatcggt gcaaagcttt
     4861 accattttgg tatattaaca tctatcatgc acaatgcatg gatgcgcaca gtatgcggcc
     4921 gtttggaaag ccgctatcgt tattccgcag gaattgttta caacaatttt ccctggccgg
     4981 aaaatccgac ggagaaacaa aagcaggcca ttgaaaccgc cgcgcagggc gtgctggatg
     5041 cccgcgcgca atttccgaac tcgacgttgg ccgacctcta cgatccgctg accatgccgc
     5101 cggtgctgct caaagcccat cagacgttag accgcgccgt ggacaccgcc tacggcaaaa
     5161 ctaattttac aaccgaagcc cagcgtgtgg cgtttctctt tgaactttat caaaaataca
     5221 ccagcctctt tgcagcggac aaacccaaac gccgagcgaa ggttgtgaaa ataccattgt
     5281 gatttatacg gcgtgttcag ggaagcctga tgtttagagt gaaggagaaa ataaaatgag
     5341 aatcgttatg ttagcgttgt gttgttttat ttgtttgttg acgtccgccc atgcgctgga
     5401 gatgtacaag tgcctggata ataacggcaa cgcagttatt accagcacgc ctcaggatgg
     5461 gatgacggat tgtgtgttga tggattcttc agaagattca gtgcctgaag ataacgcaaa
     5521 cgataatgga agtgaaacaa aagaatcggt cgcgggctca ggcaacgcca atcagcaggc
     5581 ggacgaaaaa ttgcgtcggc aatgcgccaa tttgaaagtt tatcgcgagg aagaaaggac
     5641 ttattgcgaa ggtgtgccgc agagttataa aagcggcaat gaggagatga agaaaaaggc
     5701 acaagaccgg atggcgtctt ccaacgcgag cgccagccaa ctctgcgact attacagagg
     5761 tatggtcaga gaattggaag caaaatgccc ctgataatat gacctgcgat caatccgcaa
     5821 acatttgaac ggattttgtc ccgttacggt ttgatggcct cgatggttgc ggaaacaaca
     5881 tagtcttcgg cgtggctgtc aggcagccag gtgcgaataa atattctgct ttcttccttg
     5941 agaattattt ttatttgttc aaaacccgtt tcggtcatga tgttttcaat gtcccgcacg
     6001 gaagaggctc ccgctataca gcaggcataa agggccagat cttttttgat ttcatcgggt
     6061 atcggggccg tggcgacaat atcggaaatc gctaaccggc cgcccggctt taatacgcga
     6121 aacgcttccc ggaatacttt ctctttttcc ggcgacagat tgatgacgca atttgaaata
     6181 ataacatcca cgatcccgtt cgctaccggc agatgctcca gttctcccag ccggaaatca
     6241 acattggcgt aaccgccttg ttcagcattg cggcgcgctt ttgcaatcat ttccggcgtc
     6301 atatccacac cgatcacgag accttgatcg ccgacagacc gtgccgccag aaaacagtca
     6361 aagccgccgc cgcttcccag atccagcacc gtttctccgg ctttcaacga ggcaatggcc
     6421 tgaggatttc cgcagcccag ccccatgtcc gccccttcgg gaacaacgga taaatcgttc
     6481 gccgaataac ccagcgcaag agacgcggcg ttatccggtc ttgcgcaaca ggaggcgctg
     6541 gaacagccgc aaccggattg atcggacacg gcgacttttc gataattttc caaaaccgca
     6601 atgcggatgt cgtcgttttt tttatcggtc atttttagcc tctgctttca tcgaagcaat
     6661 atattgaaga ttacagatta ttttctatca caaaattgcc gtttcaggaa tgaagaaatc
     6721 tggaggagag gatttcctgg acaaacccaa acgctgccgg tttacttttt taaatcttaa
     6781 gcagtctgac ggcttcttcc atttcggcgg cttctttcat ttttgcccgc atctgccggc
     6841 gattcctgaa ggttttccaa atgaaccgtt gcataacata attttaagca tgttctcccg
     6901 gtaatgtttt ttttaaatct ttcatctgtc ttctgggatt atcttttttt tcagcatctc
     6961 ttttaaactc agtggatagc catccgataa gcaagctgga ttgcgtatat cattttccgt
     7021 ccacgcaacg tgatgttttt ttgcatacgt gcgcaacaga ttttcaacct cctgtttttt
     7081 aagatggcgc aggttcaaag ttgttgcagg catgagttgc gccttgagat caaacaaatt
     7141 tttttgcggc agaaagtttt ctacaacggc aatgaattga aaatgctgct ccagaatcag
     7201 atgacgaagc aatatttttt tctgagccgt tattttagcc atgttgtcga aaacaataac
     7261 cggtctgtgc tgtttgattg tatttgcatg agcaatgcga taacgcatgg atttatagcc
     7321 catttgttgt gtctgatttt taattcttcg ggggatgccc agcgctttca taattttctc
     7381 gctcattttt cccggcgttt gactgaaatc gacaaagata aattttcttt cattgaccag
     7441 cttcgctatt tctctgatta aacgggttcg tcctatcccg aactttccgc ccagaataat
     7501 atttctgccg caattcagcg cttccataat tattttcttc tcgctttggc gtccgacaaa
     7561 taacataatg actctgtaaa acttcgggtg agtatggcga ttatatggtt attttttcga
     7621 tctgatcaat aaggcctctg atgtcttcag tttgtccctt tgtcccggaa atcaacgctt
     7681 cgatcgtggc gagttcttcg tgaatatagt tgaaggcctg caggcgcatt tcatcgatgg
     7741 catgattgac gctcttttcc cattgatagg caagccgcga cagattgatt tccacttctt
     7801 tcggtatgcc gtcgataaag tgccgttcga aaatcttgcg gaaaaacatc atgggaataa
     7861 gaaaccatat caggtccaaa tgaatatcaa aggttttggt aaaagagata tccggatggt
     7921 cgggttcagc ggcatcgatt ttccattgaa cttccgtcat cttcacaccc agcacgtttt
     7981 caatgttatc tcccagaaaa ttgcagaaag cgtccagcga acgggatatg gcggcgtgcg
     8041 cttttttcat ggttcccaga aaattgcgat gttcgttttt ggaaattatc cgcatttctt
     8101 cggagagcgt ctccgacacc cagacctcgt attttcggga tagtttccag agattgcctt
     8161 tccatgaagg caattcatcg accagttgac gcataacttt ttttgtaagc ggcactctaa
     8221 atttttccag ataggcttca atcagagcgc gcgtttggcg ctggtgttcg cgggaaataa
     8281 tacccagttc ctcccgcacg atatcttcat tgactttttc attcagaatc cggttgcgca
     8341 gattttcccg gttcatatcc gcctgcaacg acgaattcag cgcaatattg agatagctca
     8401 ggcagccttg agagagcgac cggactttat gattcaggat tcttaaaaac tccgtgtcgc
     8461 ggtcggcgca gattgtttta aaaatgtttt cttcaatcac ctgtttgtat tcgtccgtgt
     8521 tgtcccgcgt cgaataaaga taaaccggca gtgtccggtg aagttcccga ttaagtgttg
     8581 cctggaaaaa ggctaccact tctttttgct gatccgatga aagcagatcg actttggtca
     8641 gcaataaaat aatattcggc gtgtgcgcga ttaattcacg aatgagttcc aaatcgtgtt
     8701 ccgataaggg tcggtcggca cttaccgcca gaagcgctgt gccgacggag ggcagccatt
     8761 cttccgaagc ggactgatga tatttgtaaa cgctgcccag ccccggcgtg tccaccaggc
     8821 gcagaccgga atatttcttc agggacggca gttcaatgtc gacaatggat acatttttcg
     8881 cgttgcccgg attttccgct tcggaggtga attttacaat gtccgctaac ggaatttcgg
     8941 tgatgtttcc gtcaaagtgc cgtacgaggg cttttgcttt cgtcccatat tggagacgtg
     9001 taacggccgt ggtcaccgga atggcgccga cggacaaaac gttttgtccc agaagactat
     9061 ttaagaaaga acttttcccg gccttgaatt gtcccagcac cgcaatgtcg atgggcggat
     9121 tttcatcaag cagtaattgc gcggcttcca actggcggtt cagggaaagc atccggaaac
     9181 gcgagcaccc atccgcaatg attttcagat gacggcgcat gtcggacacg gacttttgag
     9241 gtaagacatt tcccataata aaaaaactcc cgcagaggta ttttctgcag gagttatcag
     9301 caataattgc ggttatctca ggtgaactcc atcacccgtt tgcctgtttg cgtggaccat
     9361 attcaaaaaa gagtatttag tcaatatgtt tgtcgagcaa tttattaacg gtctcacaat
     9421 catactgtta ttaagcgatt aacaaattct cggatgtctt gccggcgaag gccgggaacc
     9481 agtaaaaaat aatgccggat gccggccgtc gccggcatga cgaatgtaaa tttgaatatt
     9541 cgacatttaa taatttgatt tgatcatgtg tgagtccgct agaagtcatt cggaatgttt
     9601 ttccagtgat cgcgtatcac acgacaaaca tcgggcggca gaattttatt ttgtgtttgt
     9661 tgttgcaagc cgcgcaggct atctccagat gaatttgcgt acacgtcact ccctgctcga
     9721 tccatgtttt atttccatga gggcgccgca tgaatgcggc tgcataagtc attgtgaatg
     9781 gatgttaatt gaggacggaa aacttgagct acgcgatttt gaaatatccg cttcttgatc
     9841 catcagtaga ttcatcctat atgaaaaata atttacgaag tttgtgcacg gattataata
     9901 tgagattata taaaatgatt ttgaataaga aatgaaaacc ggaatgttta tttttttgtt
     9961 gtgttactga gttcaaactc ttttgccaac aggatggcat tttccttttc ctgttcaaga
    10021 taatccacga tgttgttttc aaccagatca atccgctttt ccagataggg cgcaacgctt
    10081 ttgagaaaga aatccagatt tgatttcaaa tcgtgctttc gtttgaacaa agtcgcatac
    10141 atcatccgct ttttggtcgg ccagagattc atgatcaagc gttccagaat aggattgtcg
    10201 acaattttaa tcgccgcttc catgaaatca aaaatggcat tgtagtaacc ttcgaagtct
    10261 tctcgattgg cacattcttc catggtttgg aaaaatggcg ctatctgatt aaaattttca
    10321 tccgaaattt tcgacacgcc cttctgagaa agaagcaaat aaagcacttt gagaatgtcg
    10381 tataaatctt caatgaattt tatggaaaag cccgctacga cagcgccgcg gcgcggcagt
    10441 aattccacca gaaatttttc attgagaatg tgtagtgctt cccggacggg gctgcggctg
    10501 acaccaagtt cctgggcgat ctttgcctcc aaaatgcgtt cccccggttt gatttgcatg
    10561 tgaatgatct tttcggcaag atattctgcg atttgatagg gtaattgatt ttgtttgttt
    10621 aatatttttg ataacttatc tttttgcacc atcatggata gccttgagtc gtttccagaa
    10681 taagctgtta gtttgtatat attattatca ttatcacaaa aaatttattt ccgatatgaa
    10741 taaatcacga atatcattat gttaataatg atataagaga acccatcttc aaataaaata
    10801 tcataaaata aacaataaat tcttaatgat atgaaaacaa aagggttgac aagaccatga
    10861 ttctgctata tgtcgattgt cgacaatcga caatgtgcgt gtaagtttcc attccctata
    10921 aaagatatta aacattaaaa tcttcaattt cgtaaggaaa tgaggggccc tgaatgtttt
    10981 tgatatgttt ttcatatcgc ttctctaatt ttcatttccc gagattgctc aatcattttt
    11041 tccattcatt aaactcacaa aacattaaag aaataaaaaa ggggggacgt tatgaggagt
    11101 aaaagaatgt gtaagattgt gccgcctgaa aaatggttaa agacacagat taccaggttg
    11161 tgtctttatt ttttaggaat gggaattgag acggcatata aaaatgtgcc gcaagcaaaa
    11221 agggaaatgg atacgttgcc ggatcaattt tcattttgct ttaacgtcgt caacggtccg
    11281 tcgatgatga ttttgaaaaa cgggcacacg gtcaggtatg ttggcgaaaa agaagcttat
    11341 catgccgatc tggaactggt gctcaagaat atcgaatttg cctatctgat gatgaccggc
    11401 agaatcagca cgcccgacgc ggtttatcat aaccgtcagt ttgtcagagg aaatctggct
    11461 tatatgatga cgatgatccg ggttctgaac atagcccaga cgctgttgtt tccgaatttt
    11521 ctcctgcggt tttacattaa aacagtcccg cggttaacta tgaagacgat ggtcaaccgc
    11581 gccaaagcat atgccaatgc aacaatagga ttattcgctt aaaagacaat cacttgagga
    11641 gggatattgt gaatatgcaa aagaaagaag aatgcccgaa atactacgaa ttttttaatt
    11701 ccgtaaaaat aatttcaggg tacaaagccc tggacaacat cgcctcggag ctcgatatgc
    11761 tcggggccag aaagcccatc atcattaccg ataaaggcgt aagccaggcg ggattgatcg
    11821 atcacgtggt gaacagtttc ggcggaacgc aggttgcgat tggcgcgttg tatgatgaca
    11881 cgcccgttga ttcttccatt gtggcggtaa acgagattgc cggaatatac cggcagcagg
    11941 aatgcgatgc gatcatcgcc gtcggcggcg gttccgtgat cgacaccgcc aagggagtga
    12001 atatcgtgat ttcagaagag gcggacgatc tgatgaaatt taccggaaac aaccgtctga
    12061 ccaagccgat gaaacctttt atcgtcgtac ccaccacggc gggaaccggt tccgaggtga
    12121 cgatggcggc ggtcatcgcc aatcccgccc ggaatgtcaa aatgcaattt acatctcccc
    12181 tgattttgcc gaaggtggct attctcgatc ccgtgatgac catgagcatg ccgccgaaaa
    12241 ttactgccgc aacggcaatg gacgccctga ctcacgcggt ggaggcctac atcggtccgc
    12301 agaaaaaccc ggtcagcgat gctttcgctt tttccgccat tgaactcatc aaggccaata
    12361 tcatcgacgc ggcgaaaaac ggcaaagaca gggatgtccg gctggcgatg gccaacgcat
    12421 cgttactcgc cggtatatcg ttctccaatt cgatggtggg catcgttcac gcgctcgcgc
    12481 atgcaaccgg cgggctgtgt catgtgcctc acggcgtcgc caacgcgatt cttcttcctc
    12541 acggtctgga gttttatatt aaaaaatcgc cggatatcgc cgcgcgtatc ggcaagctgc
    12601 tgcggttttt catggaaccg gaagattatg caaagattcc ggtaaatgaa agggagaaaa
    12661 acaccgtggc cgcgatacgc gacatgaacg acagtctgaa caagatctgc agcctgccgt
    12721 tgactctgag ccaggctggg gtaacaaaag ataaactgga agcgatagca aatgcggcca
    12781 tcaacgacgg cgctatagcg ttttgtcctg tcgaggcaag ttatgaggat gccctggaaa
    12841 tattaaaggc ggctttttaa cggatcatat aaccggcatg tatttcgatg atcaataaga
    12901 agggaggaag attatgaatg cacaggtttc aggaacggca gaacatcaag catcggctgc
    12961 aaaagaccag ggtgtcaaaa acgatgtaac ggtttgcacc aatcccgcat cgtgcgaagt
    13021 gatcgccatc taccccatcc ataccctgga agatgtaaaa aatgctgtaa caaacgcccg
    13081 gcgtgcgcaa cccgcatggc aggctctgcc tctgaaaaag aagatcgcct atgtaaaaaa
    13141 atttgcagag tatattcaaa aaaattcggc aaagctggca gaaatcattt cgcaggataa
    13201 cggcaaaacc atctttgatg ccatgtttgc cgaggtgggc cccgcaaccc tggcgacatc
    13261 atattactgc aaaaatgccg ctgaattttt agcggaccgg aaactcaagg cgggcaatat
    13321 catgctgatg aacaagcgca gccgcattgt gcgggtgccg tacggtgtcg tcggtattat
    13381 ttcgccctgg aattacccgt tcgccattcc tttttccgaa gtggtcatgg gattgctcgc
    13441 cggaaacacg gtgattttaa aggctgcatc ggaaacgcag atggtcggcc atgcgcttaa
    13501 ggagtgcatc gactatgccg gtcttcctga acatgttttc acttacatta acatgcccgg
    13561 caacaaggca ggagacgcgt tacttgatgc cggcgtggat aaactgtttt ttaccggttc
    13621 ggttcccatc ggaaaatatt taatgaagaa agcctccgaa aacctgacgc ccctggtgct
    13681 ggagctgggt gggaacgatg cgatgatcgt ttgcgaggat gcggatctgt accgcgctgc
    13741 gtgcggcgcc gtctgggcgg gcatgcaaaa cgcgggacag tcgtgcggcg gtgtggaacg
    13801 aatctacgtg gatagaaaaa tctaccatga atttctggcg gtattgaagg aaagggtgga
    13861 ggggcttcgt gtcggcgacg gcaattgtct gatgacggat atcggcgcca tgacgacccg
    13921 gaaacagatg cggaccgttc aggaacatat cgacgacgct ctggccaaag gagcaaaaat
    13981 attcgcgcaa tccaaatgcc ctcaaaactg caaggggaat tttatcccct gcactgtgct
    14041 gaccgatgtg gaccatacca tgctggtcat gaaggatgaa acattcggtc cggttgtcgg
    14101 cgttatgccc gttgaaaaca tggagcaagc gatagcctta gccaatgatt ccaatctcgg
    14161 cctgacctgc tcggtatggt cgaaaaacag gaaaaaagct gttcagatcg gccgccgggt
    14221 gatggcgggc gccgtcatga tcaacgatca tctgatgagc cacggtcttg ccgaaacacc
    14281 gtggggagga tttaaagaaa gcggcatcgg ccggacgcac ggggatatcg gttttgctga
    14341 aatgactcag cctcaggtta tcgtagacga tatcttgagc gcgatgcctt ttgtgcgcag
    14401 aaacttctgg tggcagccct tcgaaaaatt gcagtacgat ggtatgaaag ggctcatcga
    14461 cttgtttcat ggaaaaggat tctgggaacg ttttgtcgga ttaaataaat taggccatgc
    14521 tttttacaga tcatttgtaa aaaaataagg taataaaaag cgccctgatt tcgaaaaaaa
    14581 atcggggcgt ttttttgtga aaaatgacgc caaaaatgct aaaaaaaaca taacccatct
    14641 tattgatttt atatgtgaat atattaattt aaaatcggtg ttgacagacc ggcggtcact
    14701 gttgtatgaa ccattcctga catatgccat cattggcata tgtcagaggt gacattttta
    14761 ttatttatta ttgaaaaagg tggatgaata atgagtgaaa catttttatt gaacggaaaa
    14821 gagacgccct ttgaacaggg gcagacgatt gtagaagcag cgaaaaacgg cggggtcgaa
    14881 attcccacga tgtgctactt gaaaggctcc actccgaccg gcgcatgccg tatttgttgc
    14941 gtggaagtca agggcgcgcg gtcgctggtg gcggcctgtt ccacgccggt aacgccgaat
    15001 atggaggtgt tcactgaaac cgagctggtt cataaatcaa gaaaaatggt cgtggagttc
    15061 atgctgacca gcggcaaaca taactgcatg acctgtgaag caaacggtaa ttgccgtctg
    15121 caggatttgg cttattttta taaaattacc acaccgcgct ttgaagaaag cgaacagctg
    15181 tatcccacgg aagatgagaa tccttttatc gtccgtgatt tcagccgctg cattctctgc
    15241 ggacgctgcg tacaggcctg taacgaggtt gtcgtaaacc gcgccatttc gcagggctat
    15301 cgcggtagaa aaagcaagat tgttaccggc ggcgatctgc cttacaatca tgacgacagc
    15361 gaatgcgtat tctgcgggca gtgcgtccag gtatgtccgg tcggcgcgct gacggagaaa
    15421 aaggccaaag gtctgggacg cccctgggaa gttcagaaaa tccgcaccac ctgtccgtac
    15481 tgcggcgtcg gttgtcagca gttgctgcat gtcaaagacg gaaagatcgt caaggtcacg
    15541 gccgtcgaag atgcgcaacc caatcaggga cggctttgtg tgaagggccg ctttgcttac
    15601 gatttcattt attccgggga aagattaaaa acgccgctga ttcgagaaaa gaacggcgaa
    15661 ttccgcgagg cgtcgtggga tgaggcgctt gatttggtgg caaataaatt caaagagatc
    15721 atcaagaaac acgggccgga tgccgttgcc ggcgtcagtt gcgcccgcag tatcaacgaa
    15781 gattcttatc agatgcagaa acttttccgc gcggttttcg gtacgaataa tatcgatcac
    15841 tgcgcgcgta cctgacacgc tcccaccgtc gccggtctgg cgacttcttt cggttcgggc
    15901 gcgatgacaa attcttttgc cgattacgca aaagccaaaa tgattattct ggtcggcacc
    15961 aatatatccg aggcgcatcc ggtcgcttcg acttttgtga aagacgcggt gctggccggc
    16021 gccaagttga ttgtgattga tccgcggcgg acggatatgg ttgattttgc cgacgtacac
    16081 attccgctga aagtcggttc cgacgtggcg ctgatcaatg ctttgatgaa tgttctcatc
    16141 acggaaaatc tgtacgataa aaaatatgtg gagtcctgta cggagggatt tgaggaattg
    16201 aagaaaaagg tgatggagta cacgccggaa aaagccgcca aaatcagcgg tattcccgcg
    16261 gacacgattc gcgaactggc ccgcacgatg gcttccatca aaccggcgat gctgatgtac
    16321 acgctgggca ttaccgaaca catctgcggc gtcaacaacg ttatgtcctg cgccaatctc
    16381 cagatgctta tgggcaatgt cggcgtggaa ttgggcggcg tcaacccgca gcgcggtcag
    16441 aacaacgttc agggcgcctg cgatatgggc gcgcttccca atgttttccc cggctatcag
    16501 caggtagcca atgcggaggc ccgggcgaaa ttcgaaaaag cctggaatgt caaatcgctt
    16561 ccggataaac ccggcatgat gatgccgacg atgatgaaaa atttgaccac cggaaaagtc
    16621 aaaggcttct atgtctttgg tgagaatctg gccaatacgg aaccggatat caaacatgtt
    16681 gaacaagagt tgcaatccgc ggaatttctg gtttgccagg atattttccc gacggaaacc
    16741 actcgctttg ctcacgtgat tctgccggct gccgcctgga gcgaaaatga cggaaccttc
    16801 gccagttcag agcggcgcgt caaccgcgtg cgtacggcca gcgtgccccc gggagaggcc
    16861 aaacccaact ggtggatatt caaagaaatt gcaaaacgtt tcggtcacga gtgggcatcc
    16921 aacagcgctc aggaaatatg ggataacgaa gtatccgtgc tggcgccctc tatggcggga
    16981 atcaagtatg cccgtctgga aggtgacggt ctccagtggc cgtgtcctac cgccgatcat
    17041 ccgggtaccc cgactctgca caaaggcggc aagttcaccc gcggtctggg tatgctcaaa
    17101 gccatcgact atatcccgcc ggcggaagtt cccgataagg aatacccgat ggtgctttcc
    17161 accggccgtc gcctgtatca ttatcatacc cggacacaga ccggacgatc cgaggggatc
    17221 aacgatctgc tgggcgagga aacagccgat atttctccgg ccgatgccga ggccaagggg
    17281 attaaaaacg gcgagaagat tctggtgaag tcccgccgcg gcaaggttga ggtcaaagcc
    17341 agggtaacag aagaagttcc gcaagggctt gtctggatgg ctttccattt ccgcgaagct
    17401 tgcgcgaact ggctgaccaa tcccgtttat gatcccgtca cgcagacggc ggaatacaaa
    17461 gcctgtgcgg tgaaaattga gaaactgaaa taaataggga atcaaagtgt cccgctttca
    17521 aagcgggaca ctttttttgt gcagtgatta ttgtccgaga aacaacatcc gattgatctg
    17581 ttaaaagcca tcccgaaaaa caaatttttt attatgttat tattgacata atgttatttt
    17641 taatatagag tcccctcgta tttacatcag aaagagagga tcttttattc atgaaaaaat
    17701 caaatttcaa acaggtcatc acaaaaattt tattgacagt gatcggtgtg gctttttttg
    17761 cagggctgtc cggtcaagcg tttgcggaag aaaaaatttc cgttgcggtt gcggcaaact
    17821 ttatttcagc ctttaaagat atgggggccg atttcgaagc caagaccaaa attaaagttg
    17881 aaggcacttt ttcctcatcc ggaaatcttt acagccagat caccaacggc gcgccctacg
    17941 atctgttgtt atcggccgat gaagaaagac cggccaaact caacaaagac ggcttggcgg
    18001 ataatccttt tatttacgcc agagggcagg cgattttatg gtctgccaat aaggaattct
    18061 gtaaggcaaa aacctggcag gatgccctga aaaatgaaca gatcaaaaaa ctggccatcg
    18121 ccaatacgca gaccgcgcct tacggaaccg cagcgcagaa agcattggaa aaggccggga
    18181 tgtggaccga cgtgcagagc aaactggttg ttgcgcaatc gatcgcacaa tcgtttcagt
    18241 atgcatcaac ggagtcggtt gacgccggtt tctgtgccct gtcggctacg gccagtcctg
    18301 aagggaagaa gggctgtttt tatgtcctga atgaggcgcc ggaaattatt cagtcggcct
    18361 gcattctgaa aagaacaaca aaccgcgctg cggcggagaa atttgcggag tatctgcgat
    18421 ctcccgcggc acaggaaata aaagttaagt acggttatcg gtaagtggat tatcttgcaa
    18481 tttatgtgac attaaaactg gcgctggtga cgactgttgt cttgatggtt atcgcggcgc
    18541 cggttgctta cgctctggcg tacaatcgtt tttccgggaa atcctttctg gaagcactga
    18601 tttatctgcc gatggcgctg cctcccacgg tgatcgggtt ttatctgatc atcctcatgg
    18661 gaccgaaagg ttttgtcggc aaaatgtggc agatgcttac aggcggatcg ctgcttttta
    18721 cgtttatcgg cattacgatg gcgtccatca tttacagtat cccgtttgcc gtgcaaccga
    18781 tgaaagccgc cttttccaag atagaccgcc gtttgctgga atcggcctat gttctcggtt
    18841 tatccaaaaa ggcgacgttt ttccgggtga ttattcccaa ttcggtcagc ggcattgccg
    18901 ccgccgccat tctggttttt ctgcattcca tcggagcatt cggcgttttg ctgatggtgg
    18961 gcggcagtat ccccggcgaa accaaggttg cctcgattgc gatttacgag gccgtggaaa
    19021 tgatgaatta caaggccgcg ggattgatcg cgctgagttt cattcccatc agttacgcct
    19081 ttttgctgtt agtgaataag ctcaatgaag gagcgcgttc atgagtattg aagtttcttt
    19141 taaaaagaaa ttaaaatatt ttgatgtgga catttcattt tcctgcgcga aaaaaaacat
    19201 ggtcgtgatg atcggtccgt ccggcggcgg caaaacaacc atcattcgca tgctggcggg
    19261 tctggaaaaa ccgaaagaag gaaaggttgt attcggcgat gaagtctggt ttgattctct
    19321 gcgccgcatc gatgtcccgc cgcagaagcg gcgcctgggt tatgttttcc aggattataa
    19381 tctttttccg catctgaatc tctttgacaa tgcggcgttt gccgcggtgg ataaaaaaga
    19441 agttgaagat cttttcggga ttttcagaat cggccatttg aaaaaacgca aaccgcacat
    19501 ggtgtcgggc ggcgaacggc agcgctgtgc aatctgccag gcactggcga ggcatccgcg
    19561 tattctgctt ctggatgaac cgttttccgc gctggatgcc attacgcgcc gcggtctgcg
    19621 cgaagagctg aaaaatctga agggcaaaat gtccttcccg attatttatg tgacgcatga
    19681 cattacagaa gccttgtatc tggccgatga acttctgccg gtggtggacg gcaaaatcga
    19741 cgatcaatgg ctgcagcgga tgatgacccg cgagccgtcg tcggaagtcg ccctgcgcgc
    19801 cgcgcgcgaa ccgaggctga cattagtata ctagattagc tttttatgaa tatgtctact
    19861 gtgtcatttc gaagcgaagc gagaaatctt aatgatcaat attcaagata tctccccgga
    19921 agagttggac tatagcctct ggttgatatg acaaatatat cgaagaagcc gctcccgatg
    19981 aatcgggacc gacaaagaca gcgctttgat ttggaaataa tttaaggagc aaaatatttt
    20041 atgaacatct gtacctattc ctacgaagaa tacatccatc tggttaaatc attccatgga
    20101 acactggcgc ccggcctgct gatcggcggt tttatcgttg atctggccat gaagaatctg
    20161 ccggaaggag aattctttga tgccgtttgc gaaacaccgg tctgtctgcc cgatgccgtg
    20221 cagattctca cgccctgcac catcggcaat ggctggctta gtatcgtcaa ctttggccgg
    20281 tttgccgtga cgctttatga aaaatattca ggtaggggtg tgcgggttta tctggatacg
    20341 gaaaaattga ataactggcc ggaagttcgc gactggtatt tgaaaaagaa gaaaaaggat
    20401 cagcaagacg ccgaggtgct ttttgcgcaa atcaaggaag ccgggcatca attgttgagc
    20461 gttcagaagg ttcaggtgga acccgaaaaa gttcgtcgca aaaaaatggg acccgtcgga
    20521 atctgtccgg cctgcggaga ggcctatcct ttaaaagacg gcggaaagtg catgaattgt
    20581 cagggagcga ccccgtattc cagcgttgtt ccggtaaaat aatttaacaa tggggaacgt
    20641 tgctatgcta aaaacaggta tcacgcttag tcgtcttttt gaaaaaactg aaccggctgc
    20701 caggaatcga cagattgccg atttacgcat cggcttaagt tatgtcggaa taaaactgga
    20761 taacggggtg gcgggacttg ccgccgtcct gccggggagt accgcacgcg gctgcacggt
    20821 tttaaaggaa gcggggacat acatcggatc acccgcggtt gaaacattac aatatcttgt
    20881 ggaggcaaaa gacgctctgc gcagggcgat cggtgtggcg acggccaacg cgcttgtttt
    20941 gcctgaaagc ggaaaaaccg aagaccgcga agcgaccacg tacctgaatc tccaacccgg
    21001 tgaaaaggtg gccatggtcg ggctgtttgc tcctctggtc gaacgcatcc gatctaccgg
    21061 cgcgacactg accgtggttg aaaaaaatcc cgagcgtctg gaagtgctct cggcagagga
    21121 aaaacaaaag gcgctcaaag actgcgatgt tgcgattatt accgcaacga ctcttctgaa
    21181 tggtacgttt gaagaaacaa tcggcttgct gggccggccg agatcggttg ttctgatggg
    21241 accctccacg ccgctcgtgc ctgaaatctt ccacgacacg cctgtcaccc agttgggcgg
    21301 ttcaatcgtt gccgatgccg caaaagcgct gcaggttatt tccgaaggcg gcggcacgcc
    21361 ggcgctcaga cactatcttc gttttgtgaa tctgctcctg cctgacaaga gccgctaaga
    21421 ttttatatct ttttgaactg ggctatctgg cgagaaatcg cggcgaggtt gcctttttca
    21481 tcccagactt ccccgtcggc ttccagaatt ccacaggtaa tgaaacgcgt ccggagactg
    21541 catttgagcc ggtccgtttt cggcagattt ctgacgttca cggaaagctc gatcgtcgga
    21601 acccaggcgg tggcgccctg ggaagccatg gccgccgggg gcatcgaatc aataatcaga
    21661 aaaagcgaga agagatcgag aggccggccg tcacgaaatc tgaaatagcc cttgttcaga
    21721 gaaacgtccg tcagttctcc tccggtcatc catccggcgc atgcgggatc gagacggagg
    21781 tcgaggtttt ggaaaagcgt gtatgacggc ggcacagaca tcggcacaca ttcctcgatc
    21841 ggagcaagct caggggcttt tgactcatag cgggttatcg cacaatcatt gttgtctgaa
    21901 acgaatgtgc cgagcgccct gattttctca tcgccttcct gtataagacg agcctcaaaa
    21961 cgggtaaact gccgcgaacg ggtaatttcc gcaatatgaa tctcgatctt gccgggaacg
    22021 catcgggaca gataattcgc ggtgacaacc ggcgtggcct tcttgtcgct cttccggagc
    22081 atcgcgttga cgatcatggc catgaggtag cccccgttgg ggttgccgtt gaccgaccag
    22141 ttttccgaaa tcgttccggc aaagcgtgaa tgatccgatt cgctaatttg tatgtcttta
    22201 tcaaaaagat tcatgaacat ccttttcctt tatcaggagc attccccccg gtatcacaac
    22261 tccttggccc tttaccgcac aaagcgcctc gggatcaggt cctcctgatg aattcaggcg
    22321 gcctggcttc ctgaagggcg atcgcttcta ccgggcagac gatgacgcaa ttgccgcagc
    22381 ccatgcattt gttgcagtca atgacggccg tgtctccgac ggtgatggcg ttaaatgcgc
    22441 acttctcttc gcacgcgccg cagccggtgc aggtgtcggc gtcgacctgc gccagaaaac
    22501 gggagggcgc aaacgatgca tcgaaacccg cctgattgtg gagaaacatg gccgtgcaac
    22561 aacaggggca gcagttgcac atgaacatat cttccttgac gttgttgcgc accgtatgaa
    22621 ccaggcctgc ctcctccgcc acagccagcc gccttaaggc ttcatccgta ctcagaacct
    22681 cgcccacgcc gcggtcgatg gcggcctcgg caaagccgtt gatctgcatg cagaggttga
    22741 cgccgtcagg cgcgatacag tctttcgtct tgcccaggtg ttttgcctgg gtgcggcacg
    22801 ggcagggaat ggccgtgatg atccgggcgg aacggatgat gcttctggcc gagtcggcgt
    22861 caaggacacg cgaggaactt tccacggtct cttctatggg aatgacccgg agcatgggcg
    22921 ccaggttcag cttgttaaag acgggtacga tcctgggcat ttcttctttg ataatgattt
    22981 cccaaagctc gaagagctct tcggggcagt ccggcatgat gaccaccgca tcccgcaact
    23041 cgatcatggc cgggtaaaga cggtaatagt ctcccttggc cagcacccgg ctgatggctc
    23101 ctttgcgcag cagtttctgc gcgatctgac gcacccgttc ttcgttcagg cccgtcctgg
    23161 cggcaatgtc cgccacagag cccggcaagg cctccgccac ggcgacgctg tccgcattgt
    23221 cgtaaaggat ggcgaagatc ttcgccatcg tctcagatcc ggggaatccg agtttttcag
    23281 catattgttt gagcgtgtcc ggcatattcg taaaaatctc cttcctgttc aagcgaccgt
    23341 ccggtcgttt ttatcggttc ggcgctgtgc gttaatgatg atcaacagcg cttcattcgc
    23401 cgtctgcgta tcacgcagcc tcgacccggc atcgcacata acggtgcatg ggggttccaa
    23461 actgatcgcg gttggtagtt ttggtcaaac gattcacgtt ggcgccgtat ttgacgccgt
    23521 tgtaaatcag accgaaacca tgaggcatcg tgacatgtcc ggggcgcacc gtctccgtga
    23581 tttccagttc aatctcttcg ctgcccgctt ccgtggtgac ccggaccggc tgtccatcct
    23641 ttaaatttaa gactgctgcg tcgtcgggat gcatggccag cgtgcagggg cgtttgcctt
    23701 cattccaggc gggattgcgc atcagcgtgt tggcgttcat ggaggtgtgc cgtcccgcca
    23761 tcagaacgag cgggaattca gcgggcatga ccagtgccgc ctcttccctt tgtgcatcaa
    23821 tgcttttcag ttcatccagc aattccggaa tgaagagatt gattttgccg tcgggtgttt
    23881 tgatttccgc gaagttattc ccttcatcgg ttttccccac ccagaggcct tccggatgat
    23941 ctaaaatttt ccggaagatt tcttcgccca gcgttggtcc cggcgtgaat ccggctctgg
    24001 cggcgcttgc ctggaaggat tgcggagcaa cctgtaacat tccccatagg gcggcgaggt
    24061 gaacggaccc catcgccttg cccagtgttt tgcccagtat aaacggcatg gttttcatcg
    24121 ccttcggttc cgtcatcgca tattccatca gcgcctgtct gaaggcggca tgactttctt
    24181 cggctgtttt ataaagtttt tccggaatcg gcggaatcag tcccagcttg tccgccagtc
    24241 gcagatcgat ttctccctgt tccagcggtt cgccctcggg tgcgatgatc ggccggcgca
    24301 tctgaaagaa tatctccgga taggtaaagg ggaagaaagt gccgtcccag gattcataac
    24361 cggatcggga gggtagtacg taatgggaaa gagcggccgt ttcggtcatg gccagttcca
    24421 ccgtcaccag caggtccaga cgctgaaacg ctttttcata cgccgttgtg tccgcatacg
    24481 atcgcagcgg attcaaaccg gacacgagaa ccgcgcgcag tctctcggga tgatcggaga
    24541 gaatttcttc cggcatgacg ttgggcggaa aacatccgca gaccgggaag gcgttcgtcg
    24601 cgacggttct ccaggttttc tgatttcttt catcgctgtt cggaccgaag ggcacgatgt
    24661 gaccgggaat gatattgccg ccgggaacgc acagcctgcc gcagatggat tgaagcatga
    24721 cgatcaggta ggaattcagg ccgctgtgac gtcccatgaa aataccgaga tcgtaacgca
    24781 aagaggattt gcgtttggcg tacgcttggg tgaccttata aacctgatca aaatcaagtt
    24841 cgcagacttt gagcgcggcg cggacatcaa aattttcaaa cagggatttg acctgttcaa
    24901 agcccgctgt gtgattttcc atgtaatctt tattgatcca gccttctttc agaatgatgg
    24961 cgatcatgga tttgagcaac agggcgtccg tgccggggcg aatccgcaga tggatatccg
    25021 cgatcttcgc ggtttctgat atacggggat caacgacaat cagcagtttg tccgggtctt
    25081 tggagagacg ctgaagctgg cgcggcgcct gcgggatctg gtggctttcc atgccgttcc
    25141 agccgaagat cagcagaacg tccgtattct tgacatccgg accgtcgtgc agatattgtt
    25201 tcccgtgcgt gcgtccgcac acccagaacg cgccggaaaa ttcctgtgcc agagcgctgt
    25261 actggtactg cgagcccagt gtgcgcatca gggagacgcc gaacgcgacc tccaggtggc
    25321 agctctggtt tcctccgccc atataggcaa aggaccgggg gccgtactgg ccgacaatat
    25381 ctttaagctt cgcggcaatt tcatcaatgg cctgatccca cgtaatccgc tcgaagtgat
    25441 tgccgacttt tttcaaaggg tatttcaagc ggtcggcgtt atgctggtga aacgcgacat
    25501 tcatgccctt gcgacaaaag tatccttcgc ttttaggatt ggatttgtcc ggtttgacct
    25561 tgacgatgcg gttgttgtcc acgtcgactt ccagaccgca gttctgcgcg cacagaacac
    25621 agccggtttt atgcagggca cccatatctg ctcctttcgt ccggagtgta accggatgct
    25681 ttgaatgcgt tttggtcagc gggcagcgcc cagacgcagt tttttgataa tatcgaacaa
    25741 atgacgtttt tcttctttcg tcaggctttg caaaaattca agattggccc cggcgatcac
    25801 gcggatcgct tctttgctta atgcatttcc cttaagcgtt aaatgaatat gaatggaacg
    25861 ccggtcttca ggattgtttt tgcgttcaat aaattcagcc gcttccagac ggtccagaat
    25921 gccggtgagc gttgcgctgt ccagcccggt tttctttccg agttctgcgg atgtgatttg
    25981 atcttcctga tgcagaaaac ccaaaaccag cgcctgcacc ggggtgagat tgagttcgga
    26041 tactttttga gaaagaaagc ggctggccag ttgattggct ttggccagtt gaaaaaaaat
    26101 gcaatccttt tcctgcatag ggcttattat cctcgcatgc taaatactcg tatgcgagga
    26161 taataaggaa aaagagtttt gtcaagtgca aagaatcgaa tttccatcgt tatgaaaatt
    26221 tgcggtttga acggttgggg ttttttaatc ggcacagaac atttcctttt catttcagcg
    26281 gctgacttta agtcaattac ctctgaaatt atcgataaaa atttaatttg acccaaagtc
    26341 atttatgatg aggagtcccc atgtcgatct ggataggcgt tcaatcttac aatacttttt
    26401 tattatgtca aaaatgacat aaggcattga aattattagt tttttacgtt gacaacatct
    26461 gctgcttgat gtataaatgg cccgtcataa atgcgttatt ctttggaaaa aacgttgtgc
    26521 tcaatccaag gcctgcttct tccaaaagct tttcctgaaa atgcaaattc aaggaacaaa
    26581 tcacacagga aagccatagc gcaaaaagaa tatttcaata ttgatgaaag gagaaaaatt
    26641 catgaacgtc agccgaagag gtttcttgaa aatttcaggt gccgtggcag cggtcagcgg
    26701 attgggcatc agtctcaaat ccatagccgc gcatgccagg gaactgaaaa tcatgcatgc
    26761 caaagaaacc accaccgttt gtccgtattg ttccgtcgga tgcagcatca tcgtgtccgt
    26821 gcgtgacggc aaggttatca acacagaagg tgatcccgac agccccatta accgtggagc
    26881 gctgtgcagt aaaggcggtt cgatctatca gatggctgtc aacgagaacc ggctgggcaa
    26941 accgctttac agagcgccct acgccagaga atggaaagag gtggaatggg aatgggcgat
    27001 tgacaaaatt gcccagaacg tcaagaaaag ccgtgacgcc agttttaaaa aagtcaatgc
    27061 caagggcgaa gttgttaatc gcacggaggg cattgcctct gtgggaagcg cggcgatgga
    27121 tcttgaagaa tgtttcacct atcaaaaatt cttaaggggg ttgggtctgg tctatattga
    27181 acatcaggcc cgtatctgac acagcccgac tgtaccggct ctggcagagt cgttcggacg
    27241 cggcgcaatg accaaccact gggttgattt caaaaatgct gatgtgattt taatcatggg
    27301 aagcaatccc gcttccaatc atccggtgtc atggaaatgg attcaggaag ccgtcgataa
    27361 acgcggagcg aaaattatct gcgttgatcc ccgcttcacc cagtcggctg cgaaagcgca
    27421 cctttacgca ccgcttcgtt caggaacgga tatcgctttc ctgggcggca tgatcaaata
    27481 tattctggat aataaacttt attttgctga atacgtcaaa aactacacca acgcttcttt
    27541 cctggtcaat cccgatctga aacttcccgg agagaacaac ggggtatttt ccggtttgac
    27601 agacggcaaa tatgataagg gtacctggtc ttatcagaaa ggcccggatg atgtgatcaa
    27661 gaaagatcct atgctgtcgg atccccactg cgtattccag cttttgaaaa aacattattc
    27721 ccgttacacg ccggatgttg tttccaggat tacgggaaca cccaaagata aacttcttga
    27781 agtttacaaa ctgtacgctt caaccggcaa acccaataag gcgggcgttg atctttatgc
    27841 catggggtgg acgcagcaca cggtcggcac gcaaaacatc cggaccatgt gtatgatcca
    27901 gcttttgttg ggcaacatcg gtatcgcggg cggcggggtc gcggccatgc gcggcgagtc
    27961 caacgttcag ggttctaccg accacggtct gcttttccac atctggccgg gctatctggg
    28021 aacgcccacc ggttcactgg caactctgaa agattttatt gagaaacgga ctccgaaaac
    28081 caaggaaaaa gattctttga actggtggaa gaacacaccc aagtatgtcg ccggtttcct
    28141 tcgctccatg tatggatcgg atatgactct cgaagatgcc tatgaagccc tgcccaaact
    28201 tgatgacggc gccaactact cgtggctgac gattttcgat cagatgtaca aaggcaagtt
    28261 caccggcttc tttgcctggg gcatgaatcc tgcctgttca ggcgctcatt ccaataaggt
    28321 tcgtcaggca ttgggcaaag tggactggat ggtgaatgtc aacctgttcg acaatgaaac
    28381 cggttctttc tggcgcggtc ccggaatgga tccgtcaaaa atcaaaacag aagttttcat
    28441 gctgccgtgc gcgtcttcgg tggaaaaaga aggcagtatc gccaacagcg gacgtctgca
    28501 gcagtggcgt tataaagcca ttccgtatta cggcgattcc aagccggatg gcgacatcat
    28561 gagcgaaatc ttttttaagg tcaaaaaact ttatgaaaag cagggcggtc ctaatgctgc
    28621 ggccatcact aaaatgacgt ggccctacgg caagcatgag ggcaaggaat tccactatga
    28681 accccgtaaa gtggccgcgg aaatcaacgg tttcttcctt gaagacaaaa ctgttgagaa
    28741 tccgaccaag aagggtgagt tcaagtcctt taagaaaggc gatctggttc cgacgttcgc
    28801 ctggctgcag gatgacggtt ccacttcatc gggctgctgg atctattgcg gctccgtgag
    28861 tgaaaaaggg attctgccca tgcgtcgcgg tcaggccgat ccgacaggtc tgggacttta
    28921 ttccgaatgg gcctggtcct ggccggtcaa tcgccgcatc atttataatg gcgcgtcagt
    28981 tgatttgaac ggcaaaccct gggatcccag ccgcgctgtg attacttggg atgccaaaga
    29041 agaaaaatgg aagggcgatg ttcccgatgg agccggcaat cccggcaagg ggcgtccgcc
    29101 gtttatcatg aaaccggacg gcgtggcctc tctgtatggt cccggtttgg ccgacggtcc
    29161 gttccccgag cattacgaac cgctggaatg cccggttgag aagaatctgc tttcaccgca
    29221 gaagaacaat ccggcgatca agcgttttga taaagaaggt gtcggctccg acatggatgt
    29281 gtattccggc gtcggaacct gcgatccccg cttcccgttt atttgttcca cctatcgggt
    29341 cagtgaacat tggcagaccg gtgttttaac ccgctggtgt ccgtggctgg cggaaatgca
    29401 gccgggcatg tttgtggaaa tgagtcagga actggcgaag atgagaggta ttgaaaccgg
    29461 agataaatgt acggtaagta cagcgcgcgg caaagtggtc tgtacggcca ttgtgacacc
    29521 ccgattcaag ccttttgata ttgacgggaa tgaaatccat gaaatcggta ttccctggca
    29581 tttcggttgg attacgacga aagaccgtcc cttcaataaa ggggataagg ttgcggaggt
    29641 attcaccacc ggtgacgcgg cgaatctctt gacgccgaca atcggtgatg ccaacacgat
    29701 gataccggag agcaaagcct tcatggcgaa tgttgtaaag ggggtgaagt aaaatgactg
    29761 agaagattaa attatacgat gcttcgaaat gtacagcgtg ccgcggttgc cagctggcct
    29821 gcaagcagtg gaatggatta aaggccggac agacggaaca tacgggcacg tatcagaatc
    29881 cgccttcttt gcagcacaat acgtatatga ttattcactt tcaggattat gtcgcaccag
    29941 aaggcgatgt cagatggatt ttccggaagg aagcctgcat gcattgtacc aatgccgcct
    30001 gcgtgacggt atgtcccagc ggggctcttt actataataa agaattcggt acggtgggta
    30061 tggaccggga aaaatgcatc gggtgcaagg aatgtatttc cgcctgtccc tttgaagtgc
    30121 cccagtacga taaaaagaca gagaaagttt ttaaatgcga tatgtgcgaa acaagaatta
    30181 ccaacaattt gactccggcc tgcgtcaagg cctgtccgac cggcgctctg aagtggctgg
    30241 ataaggaaga ggccgtcaaa actgccgaag cccgcgtaca gcaactgggc ggcgacgcca
    30301 atgtatacgg cgataaattc gtaggcggca cacatatgat gtatgtgttg aaagagaagc
    30361 cggaagttta cgagggtatc aaacaggatc cgaaagttcc gttctccctg accgtatgga
    30421 agagctggct caagcccttg agccttttgg gagccggggg cgtgatcggc ggagcgcttc
    30481 tgcattacat tctgcacggt ccgcacaagc cccatccgga gcattacgat caagatacga
    30541 agcagggagg tgcgtaagat gaaaagtaaa atggtaatgg tgacatcaaa atatgagaga
    30601 ttcgttcatt ggctgttggc catctcctgc cttcttttat gctataccgg actggggatt
    30661 atgtacaaag agttgaactt tttgggtttc attttcggcg gtttgaaagg attggccacc
    30721 gttcatgaca ttatggctgt ggtttttgcc atatcgttgg tattggccat agcgatgtgg
    30781 tggaaagaag cgggcctgtt tgtttttcct gaagactggg aatggatcaa ggcagccggc
    30841 ggatatttgt ggcatctgga tcacgttccg gagaccggca aatacaatcc cggccagaag
    30901 gcgttctttc tgaccgtcgc cttgttcgga ttactgatga tctgcaccgg atttttcatg
    30961 tggtatcccc acgtccttcc ggcggcattg atggattggg tttatgtcct gcatgtgctg
    31021 ggttttgtgg tcatctttgc gttcttcttt gttcacctgt atttggggac cattggtaat
    31081 cccggatctg tttccgcgat gatcagcggg aagatggagt tgccggtatt gagaatgctg
    31141 catccgaaat gggtcaaaga gatggaacac gaaggcaaac tcatgatcgc cgacgataaa
    31201 aagtaagtcg aattgaaata taattcaaaa gggtctggaa tttttccagg cccttttttt
    31261 ataagtgctt tagtccgctt caacggaggc ggtatggctt attcccgtca gatttttaaa
    31321 cggggaattc gttgaggaag gctcgctccg tttcataggt gccgatcagg atcaattcat
    31381 cttccttgcc aagcctgatg tccggttcag gattgatatg cgtttcccct ttgcgcgaca
    31441 gggcaacgac cgtgcagccc gttctttctc tgatgccgga gtcggccagt gttttccccc
    31501 agagcgatcg gggggtattc agatggaaga agttcaaccc ttcggccagc agcagtgttt
    31561 cctcattcat caggaaatta aaaatagtat tggcgcccag cgacgcatac gacatgacga
    31621 aatcggcgcc ggcgcgatgc agtgtggata tgttgcggtc ttcgctggtg cggctgagaa
    31681 tctgcatatc cggccgcagg ctgcgcagat attttgtgag ataaatattg gtggcgtcgt
    31741 catgcgatgt gatcagggca gcgggcgctt tttcaaacca ggctttctcc agagtatgaa
    31801 tgtcggcggc atccccatat acgaaatgcg aatcatcctc atttttccgg cggctttttt
    31861 caatgatcag atagggaatg tcccgctctt gaaagtgatt ggccacggcg cgtccaaccc
    31921 ttcctccgcc gacgatgatg accggatcgc cggcaatttt gcagatgtga taaaatgaat
    31981 agacgtcatc atattggaca agcgaggatt ccaggcccgc cagcaccagc acgctggttc
    32041 gattgatttt ataatcggat ttcggaataa tgaattttcc gcgttcccag attcccacca
    32101 ccgttaagcc gaaggtttcg cgaagtttac tttcggcaag cgtctgaccg accagagggg
    32161 tgccgatgac cggtgtctgg gcgataatca attccttgtg ccggccgatg atattggctt
    32221 tgcattctcc ggcgacagtc caactggcca gagattctcc catgatatcg taaagctgca
    32281 agacccgtga gcttcccgcc agttgcagaa tatcaacgga atgaggggac tccgccgttg
    32341 tgataatcgg aatatgttcc agatcctccc ggagggttag ggtgatattc gtgttcatct
    32401 ggtcgctgtt tgatgccaca atcaactcgg cattctgtac gcgcattttg ttgtatgttt
    32461 cgggatcatc gatattgccg agcgcaacgc gaatatcccg gtcgtacaat tccagagcct
    32521 tggccagatc ctccacgatc accacatagt ttttattgta gcttttcagt ttttcaatca
    32581 gagcctccgt taccggatcg aaattggtga taatgacatg atttctcgtg tccacgggaa
    32641 gttcccgagg cgcgcggttt cgggtttgcg cttccatcca gggcgcaaaa aagaatttga
    32701 tgaagacgaa aggcagaaga atcagcagca gaataacacc ggaaagcaga acgatgatgg
    32761 aaaaaagtct tccgatgtca ctggtgaacg taatgtcccc gaaccccagt gtggacatga
    32821 ccaccatcgt ccagtaaaat ccggtgatcc acgagtgttc ctgtccttcc atggccatga
    32881 tgtaatggaa caggatgctg taggtggtaa tcagcgcaat aatcgcaatc agatatttca
    32941 ccagaagacg aatgttggct ttgacaattt tactctcgac aaaagaactg acgactgcgg
    33001 gtaaaaattt catacggttg gcatccttga gacggaactg attttgagcg acgtgatttg
    33061 tctgcgtatt gcaaagtata gaaagaaccg ttttgcctgt caatggaaat catcacgggc
    33121 aaaaccgcac cggaccctcg ccttcgccaa tcgacattat gtctgtgcct cagcctttgg
    33181 aggattgaat agaagcagga tcgcggacag ggtgaaatat gccagggccg tcatataatg
    33241 cgcctgagac tgaatgtgcc acggcagata cagttcgccc gtcggcaaaa ccgagtgaat
    33301 gatgccgaag agggtcagga tggcgctgac cgcggcgcat aaagccgcac gtttcgcctg
    33361 atggtcgatc aagaaggcca gcatgcttcc ccagagaagc cccgtgatga tgaatccgtt
    33421 gctgagcatc gtcagcgtct gatgcatgat cagcaatcgt ccggggagtt tttccaggat
    33481 gatgcccgac gcgcccgtga actgtcccag aacaatcagc accatgctgg cgataaccgg
    33541 cagaatgctt aagctgaccg ccggggagtg ggcggccggc gaatccttat acgcctgccg
    33601 catgatctcg aaaccgataa agatgaaaat cggcgcaatc acggcccgcg gaatcaggct
    33661 gacaaaaacc gaaagcagcc cgatgatgga gcagacgccg atcaggatgc ctgtcagcag
    33721 cgtataccac caggtggccc ccattttttt gtaagccgga tgaccgatgt agggtgtggt
    33781 ctgcgccaca ccgccgaaga aggacgcgat cagcgaggac agcgcctccg tcagcagaat
    33841 atcgcgggtg cggtaatcgt ctccggccag acgcgcgctt tcggtgttgt tgataccgcc
    33901 gatgatggtc agaatcccga agggaatggc gatggtcaga taatgaacgc tctgcggcag
    33961 ggttttcaga aattcgacgg acggcaccgg cagagcaaag tgcatgtcca gagtcggcgc
    34021 ggcaaactcc ggcaggtatc cgctgaatcc caggaaaaaa tgaattccgg tgcctaaaat
    34081 taccgccgcc agcacgccgg gcatccgtcc gggcagttgc attttgctca tgagagttgt
    34141 gaagatcagc cccagcgcca ccatgccgac gattgcttca ttgaaaattt caacaagcgg
    34201 caagaagccg aggagtagaa gaccgatacc ggccagcggc cccagaagtc cggcggtggg
    34261 aataacgcgc tggatccatc cgccgaaaaa agacgtgacg atcttcacgg cgccgatcat
    34321 gaaaagggtg gccatgccga tatgccaggt caattgcgcg tcgcgtgtcg cgatatacgc
    34381 ggggcccata acagcgtagg caatgccgat ggtggatggc gtatcgattc ccagcggcat
    34441 ggccgtgaca tcggtcctcc ccgttttttt tctcagacgc agcgccagcc aggtatagac
    34501 gatatctccg ataaagatgc cgaccgccgt accgggaatc atgcgggtga ggatcatgtc
    34561 cgccggatag ttgaacgtaa aaaccaggat ggcgctgaaa aaaatcagtg ttccgacatt
    34621 atccagaaat aaagcaagaa aagcggtggt ctctccccag aatctgttct gaacggttgt
    34681 gttttgtgta tgcattctat ttcctcaatc ttccgttttc ctgagtgtca cgtatgctga
    34741 aggttatcgt ccatctatgc cggggagaaa tcttgatatg caataatgtt gatattgctt
    34801 atacgataac tgtataaaca aaaaaacgct gtttgacaat gcgttttccg ttaaaggaat
    34861 ttcatcaaaa gcatggatta agcaatgatt ttccccgtaa tagggatgat aggatttttt
    34921 ttaatagacg ggccatgctt cggaattact ttttacgtct tgaggctttc tcctgctcaa
    34981 aaagcaattt gcgcagggtg gcgcgataac cattgagtgt ttcgcgatat cgttcaatga
    35041 gcgccagact gtccggggtt gcactcgcca gataggaaag caccagttct ttcttgatct
    35101 cgatgtgctt tttgatacgt tcttcatcaa gagaatcata aaacatttcg atcatttttt
    35161 tgcgcagggg acggcaggtg tgtgtgatga ggataacgac aatgttgtta cctgcttccg
    35221 cgatggcttc ctgtgacagg agctccagat caatcaaatg ttttttatcg tgcaaattgg
    35281 ccagctcttc atcaaaaata tcaatcaagg ttttaatatc gcggggcgta attttgggga
    35341 tggcgagtct aagcatctcc ggcaggaaaa aggtccagaa ttcccagatc tcatccagaa
    35401 tataaggatc cgccacccat tcattatccg gctcttccgt ttgcagaaaa aacattctta
    35461 gaaaatcgat gctggcgccc ttcatatagt cgcggacata aacgccgtcg ccctgccgga
    35521 tggtcagcac gttcatcagt tccagatgct tgagggcgat tctcagcgac gctctgtcca
    35581 catccatatc tttggacagt tgccgttcgg aaggaagctt atcgccgggc ttcaactgcc
    35641 tgctgaaaat ctgccggacc agaatattca taaccctctc atgtgactga agttcttcca
    35701 taatgactcc ataaggaata catgcccttc aaaaaaagta aaaaatactt gacatgtggt
    35761 aaatatatgt tattcattgg tcaaaccaat tatacgtttt taatatacca cctttgctcg
    35821 gcatgatcaa gtacgaagtt gatcaattga aaagaggtct tcaaaacaag acgtcttttt
    35881 ttgtagatta aatataggtc cgtcttctaa ttacgaaatg caaactatga aaatggatta
    35941 taaatattta aaaatacaaa aagaaaacca tagcctttgg gtggaaatga aaaatccgcc
    36001 ggttaatttc ctgaccgtgg cgatgctcga agagctcttt gacctcgtca aacaagtctc
    36061 ccgggatcct tccatccggg tatttatcct gacgggcggt atcgaagata tttacatcat
    36121 gcatttttct attcccgaac ttttcacgtt atcaaaggac aacaaaaaaa tacggctcaa
    36181 ccttttcata aaaaccagac tgacagctgc tatcctgaag cactttacaa cctgcacgaa
    36241 cgggttgatg gatggcttcg gatggtatga gaagctggtg ctgaaatttg ccaaagtttt
    36301 gagcggctat tcatccagcc tgtatttgtg gttcatcatg caaaggcttt acatggcgat
    36361 tgaacggctg aataaaatta ccattgccgc gattaacggc aactgcaacg gcggcggcac
    36421 cgaattatcc gcgtgtttcg atttccgatt tgcggtgggc gatcagggat ttacgatcgg
    36481 acaaccggaa gtcctgatta acattgttcc cggcggcggt tctacgcaaa ggcttccccg
    36541 gctgatcggc aaagccaaag cgctggaact gatgttgcga ggtaatcagt gggacgctga
    36601 ggaggcacac cgcgtgggtt tacttaccgg tttttttaat aaagcggaat tcaagaccaa
    36661 agtacaggaa tttgccgatt tgatgagcaa gcgtcccatg acggctgtgg atgcgatcaa
    36721 gaagtgcatc cataacggaa tggaaacaac tataagacac ggtttgagca tcgaaatgga
    36781 acagagcatt cgctgtctgg atacggatga caccatgaat gccatgcagg catacattcg
    36841 ttacttggat caacatatta atgccatgga tcctcagaag atgacgactg aagaaattaa
    36901 tcaagttgta caggataccg tgaagcatat ggaagaaggc aaactttata aatttaaaca
    36961 ttgatgagga tacgggccat gaagaaaaca accatgggcg caaattcaaa aatcaattca
    37021 tcagcgaatg caatactcgt gactggcgcg tccagcggaa tcggttgggc aacatcgctg
    37081 gaactggctc aaaaggggtg gcgtgtattt gccgccgtta gaaaagaggc cgacgccaaa
    37141 aaactgcgtg acgcctcttc caataaagtt acgcccgtga tcatggacat tgtcgactat
    37201 gacagtgtga agcgtggcgc gagcgagatc gaaaaggccc tcggcggcgc gggcctggac
    37261 gcgttgttca acaatgcggg gatctcggtc cagggcccct tggaaatact gcccattgaa
    37321 ttatttgaac aacagatacg ggtcaacgtt ttcggtcaca tcttcgtgac gcagaccttt
    37381 ctgccgctcc tgcgcaaggc gcaggggaga atcgtgttca cgagttccga gagcggcagg
    37441 gtcacattac cgctcatggc accatactcc tcatcaaaat ttgctcttga ggccgtcgcc
    37501 agcgctctcc ggatagaact tcgcccgtgg aaaatcagag tctcgtccgt ggaattgcag
    37561 accattaaaa cacccatgtg ggaaaaaata gatgccacca ccgaaaaatt atttgcatcc
    37621 gtaccgaaac aggcccgaga cctctataaa gacgagctaa ggaccctcag cgtctttcca
    37681 aaatggcagg cggaaatggg gatatccatg aaaaaggccg tccgggtgat cattcgcgcc
    37741 ctcagcgtaa aaaggccaaa ggctcgctac ctcgtgggtt gggaggccag gttcttagtc
    37801 tattgccaca atgttacgcc aatctggctg atggactggt tctcaagtaa aattatgata
    37861 ctgctgggta aatttataaa accgaagtga acgaacaatc gggagccgat aatctgcggg
    37921 gatcgttcgt tttaaagaat tgttcatgcg gcaaatatat gagggcaata atatatttgt
    37981 tgtaaagaaa gatattcaaa aaatggagaa ataatatgcg agacgtctat atcatcgggg
    38041 ttgatacaat caaattcggc aaacacatgg ataagagcat caaggatctg gcgattacga
    38101 ctgccaccgg atgtctcaaa gatgccggtc tggataaaaa agatattcag gctctctggt
    38161 ttgccaatgc cggctggggt gaaggagggc agatgactat tcgcggccag gtcgccctca
    38221 agggcatggg aatcgaaggc attcccatca ccaatgtgga aaacgcgtgc gccggtggat
    38281 ccacggcgct gcatcatgcc tggtacggag ttgccggcgg tttgtatgat atcaccatgg
    38341 ccgtcggcgc ggaaaaactt tatttttcca ataagaatct cgtcttcttc ggatttttgg
    38401 gaggcatcga tatcgagaac gttctgaata tcgctaaaac cctttctgtt tttactctca
    38461 cgccggaaga gaaggtaaaa gtggaagaat ttaagcagcg ctaccaggcg gtgaaaaagg
    38521 cggcaaacaa tggcggcgtg aaaaaagcca aagcaaaaga atcaaaatgg aaggcctatc
    38581 gagacaagtt taccgccttt attgttctgg gtgaaaaaat cggttacgat accgtgcggc
    38641 agctggccag gatgtcatca ggagatcact cgccgttcat ggatgtttac ggtttcgccg
    38701 cccgccagca catgaaaaaa tatggttcaa cggcggaaca gatggccatg attgcctcca
    38761 agaatcattt caattccaca ctcaatccca acgctcaata tcgctttgac gtacccgtgg
    38821 aaaaagtttt atccgaccgc atcgtctcct ggccgctcac gcgctccatg tgcgcgccca
    38881 ttggcgatgg cgctgcgtca gctattttat gttctgaaga catggtgaaa aaactcggcc
    38941 tgacccatca agctgttaag atcagggctt ccattctcgg ttcggccatc agaacatctt
    39001 ttgattccga cattccggaa atcggcgaga gactctccaa acaggcttat gaaaaagccg
    39061 gcgtcggtcc gcaggacatc aacgttgctg aagtgcatga tgcttcggcc ttcggtgaag
    39121 tggtgcaggc ggaaaatctg ggcttctgtc caaaaggcga aggcggaatt tttgccgaaa
    39181 aaggcgaaac aagcctgaag ggccgcattc ccatcaatcc cagcggaggc ctgatttcac
    39241 gcggtcatcc catcggcgct tccggtctgg ctcaggtcca tgaactggtt acccagttgc
    39301 ggggcaatgc cggaaaacgt caggtgaata acgccagact ggccctggcg gaaaacggcg
    39361 gaggcgcctt ggggaccgag gaagcggcca tgtgtattca tattcttgaa gcaccctcat
    39421 cctgaaaaga tttacgtcac c
//
LOCUS       DAOOLB010000016        38164 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_833_length_38164_cov_47.937679, whole genome shotgun sequence.
ACCESSION   DAOOLB010000016 DAOOLB010000000
VERSION     DAOOLB010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 38164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38164
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_833_length_38164_cov_47.937679"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..653
                     /locus_tag="PLF77_03690"
     CDS             57..653
                     /locus_tag="PLF77_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC07990.1"
                     /translation="MIKKKSYVFVWIALILTIAGCGGLHFSQLAPEAIDFHPQSIAVF
                     PIEVWNHKEPDNRTVVQQIVAGSLIAKKLFTSVTDAEKLQKQMQNDELLKKTAEEYFS
                     KLQLLKFSDADLSRKIGDILQVDGILLLSVDEWKYFVEADKKMAQVGLTMELYDVTNG
                     KLMWKANHTIKKDYVLIKPGLPDMARDVANKMVYYMPH"
     gene            701..922
                     /locus_tag="PLF77_03695"
     CDS             701..922
                     /locus_tag="PLF77_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPC07991.1"
                     /translation="MKEDVQKAIDTIRPSLQADGGDVELVDVSDDGTVKVRLTGACHG
                     CPMAQMTLKMGIEKVIKKLVPGVKEIVAV"
     gene            1034..1213
                     /locus_tag="PLF77_03700"
     CDS             1034..1213
                     /locus_tag="PLF77_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPC07992.1"
                     /translation="MAYVITDECIACGSCESECPEGAISEGDDKYVIDPKLCNDCGTC
                     ADQCPVEAIVPGEGK"
     gene            1338..1988
                     /gene="tmk"
                     /locus_tag="PLF77_03705"
     CDS             1338..1988
                     /gene="tmk"
                     /locus_tag="PLF77_03705"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HPC07993.1"
                     /translation="MIKAKKFITFEGIEGSGKSTQIKLVAEYLQNQGVELIVTQEPSG
                     TDIGRQIAGILFHRRHNHLCAEAEMFLFCAARAQHVRDVILPALRQGTLVLCDRFSDA
                     TYAYQGFGRGLDQKFIGAVNDYSAMQLKPDMTLLFDLPVETGLERATARNNRLADPAA
                     TDRFERETLDFHRRIREGYLTILNNEPERFRLIDATRKIPVIQEEVRRHISSFIKV"
     gene            2022..3002
                     /gene="holB"
                     /locus_tag="PLF77_03710"
     CDS             2022..3002
                     /gene="holB"
                     /locus_tag="PLF77_03710"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HPC07994.1"
                     /translation="MSLGNIYGHEKKIDMLRKTLARRRIAHAYLFSGPSATGKKTLAV
                     EFVKAVNCISSDDRHDACDACPSCRKIQHASHPDIFFVEPEGQFIRIASIRQIQERTT
                     CRPLEAKKRAFIIDDADRMNEESANALLKMLEEPSPANMLILITTRPHAMLQTIISRC
                     QHIHFNPLAVETVARFLVSEKNMEHSRAMLLAALSGGTIGNALELNQEEVVAYRTELF
                     NTLALTDRKEPFSLIHFASFLGQKKKEIRQSLNILNTFFRDVLVFKEVGQKGMLINQD
                     NTPLIAACAARLTGKQILQNIAQIEKAGDIIEQNVNKSLTLETMAFKLNY"
     gene            3020..3778
                     /gene="ricT"
                     /locus_tag="PLF77_03715"
     CDS             3020..3778
                     /gene="ricT"
                     /locus_tag="PLF77_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPC07995.1"
                     /translation="MLVNIIGVKFKKEGRIYNFDAADLILHRDEQVLVETDSGLALGT
                     VVTDVKRCEPSAVPANLKKIIRKVTDDDLKIRKENDALEQEAWKFCLERIRKRELSMK
                     LVNVDCLFEKNKIIFYFTADSRVDFRELVKDLVQKFKTRIELKQIGARQETRITKGLG
                     ICGRMVCCAGILQNLERVTVKMAKEQSMSLNPEKISGLCGRLMCCLAFEYDGYAGKKK
                     AAKAAKTSGSATEAQEEKQTLDLEKMDDIDHLQS"
     gene            3796..5691
                     /gene="metG"
                     /locus_tag="PLF77_03720"
     CDS             3796..5691
                     /gene="metG"
                     /locus_tag="PLF77_03720"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPC07996.1"
                     /translation="MPKSFYITTPIYYVNASPHIGHAYTTIVADVLARYHRMIGDKTF
                     FVTGTDEHGDKIAEAAQKAGLTPKQYADNISAQFRALCPQLAISNDYFIRTTDANHIE
                     TVRYILQKVYDAGDIYFGSYGGYYCFGCERFLTEKEIVDGKCPEHGREPQYIEEKNYF
                     FKMSKYQDWLIDHIKKNPEFIRPERYRNEALAFLRDPLEDLCISRPKTRLEWGITLPF
                     DEQYVTYVWFDALINYVTAVGYPDGEHFKTFWPVAEHLIAKDILKPHGIYWPTMLKAA
                     GIEPYQHLNVHGYWNSDQSKMSKSLGNIVRPLDLKDKYGLDAFRYFLLRDMVFGLDSN
                     FSEDAFVGRLNSDLANDLGNLVSRAITMAIKYCGGRVPAVAGDEKNPALREAALKAVL
                     DVEASFRDMSLHKALIAVWDFINITNKYIVENEPWSLAKDPANGKKLTAVMYDLLSAL
                     HAIAVMLSPFMPQTAEKILRQIGCEKTEGLKLDDIKTDNALKTGQVLTRSDALFPRIV
                     QQKEKPEDQKAAMTDLKPEIDYETFSKVDLRAATILAAHAVPKSNKLVKLTVDIGEER
                     TVVAGIGKDYKPEDLIGKQIVIVANLKPAKLMGVESRGMLLATDTPEGLTLLTFDRAA
                     KTGGKIR"
     gene            complement(5730..6428)
                     /locus_tag="PLF77_03725"
     CDS             complement(5730..6428)
                     /locus_tag="PLF77_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HPC07997.1"
                     /translation="MKKLTAIILFVLCLIPVVIFAKNASVEDQFKKSFPKNKFESIVP
                     TSIKGIYEVYSGNQLYYYMPKDEIVLYGTLVSKSGVNITRDSLLKKMAQKMEKLPLEN
                     ALKIGNGKKIIVEFMDPHCYHCRQAYQFFSQRKDVTMHVFFFPLSKESADQIKHIYCS
                     KDVLKTYDDVMKGKLDNNASLNVCADAKIDEKIKTHVQLASQVGLRGTPLFYIKGQVV
                     DGFDVALIEKLLKD"
     gene            complement(6540..>7247)
                     /locus_tag="PLF77_03730"
     CDS             complement(6540..>7247)
                     /locus_tag="PLF77_03730"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HPC07998.1"
                     /translation="EIRHLHVFRKEIFWNGQKQSQVIYEDITLRRRAENRLNETLESL
                     RQSIKITIQVLGTASEAKDPYMAGHQRRVADLARAIATEMKLPQDKIEAIRMASAIHD
                     IGKISVPAEILCKPAILSDIEFSFVKNHPRYSYDIMKEVESPWPLADIVYQHHERMDG
                     SGYPLGLKNDEILIESRILAVADVVEAMISYRPYRPALALEMALAEIEDNAGTLYDAE
                     VVKACLRLFRDKNYQMS"
     assembly_gap    7248..7347
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7348..10207)
                     /locus_tag="PLF77_03735"
     CDS             complement(<7348..10207)
                     /locus_tag="PLF77_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC07999.1"
                     /translation="MESKDGKEDKNVNALLSLPDSTKTLGKKSKSREVNPSGLKRKVS
                     QDTRRMPQKDKPAISGASPKTAGHKNGKESFCGPEEKYALAFKYSPIGICISSLQDGR
                     YVEVNEVYSNFLGYRREELIGRTSSEINLWVDMAERDALVRELLEKGTVTDFQLRFRD
                     RNGNVHWGNVAAALVTIGQELYVLTQTQDVTDKKKIKEQFEKSEAKYRLLAENLTDVI
                     FTIDMNFNYTYASPSTYHLLGYTAEELMTMKVENLVDPETLAWFMEMLAEEMEIEKRP
                     DRDLKRSRVLEYQHIRKDGSTIWVENKITFLRDQDNNAIGIIGTVRDIAERKRAEEQV
                     CKEIDFNRSVLDSLTVPFFMFDTASMTFFRWNKTFSAVSGYSDEELGQMRPSALISEH
                     QKDFLLAKFEEFQRTGHIAFEMPVLSKNGSVTPYLLSGNLLHHDGKPFVVGMGIDITE
                     RKKAEESLRRSEEKYRTILENMEEGYFEVDLSGNFTFFNDATCRHFGYSREEMTGMKN
                     RQYMDEKNSKALYIVFNRIYETDQPVTNLEWQIIRKDGAKRDIEGFVFLLKDVAGTPI
                     GFRGIVRDITERKQVQEKLHRERQLFKSVTEQSSDIIIIADTRGRITYENPAVEKILG
                     YRVEERLGASGYDLVHPDDAQIVADAAGKLFHNIQAPVQKGEIRLHHKNGSWRTFEVT
                     ASNLVKDHVVEAAIVNMHDITARKRSEELIRQSEAKYRLLADNMKDYVWLMDLNLKIN
                     YISPSVERLMGYSLEDFKKFSLDKFLTVQSFQAAMDFFAIEMPKALGAPPDYVLERSL
                     ELEFCCKNGQTVWGECNFSLIRDDKGKPLSLLGVSRNINERKQMEDALRKSEENFRHS
                     LDDSPLGVRISTVEGKTLYANRAILDMYGYDSKEELQNTPLQERYTPETFEAFKERKK
                     KRLKGQFGPSEYEISIIRKDGEIRHLHVFRKEIFWNGQK"
     gene            complement(10459..13659)
                     /gene="carB"
                     /locus_tag="PLF77_03740"
     CDS             complement(10459..13659)
                     /gene="carB"
                     /locus_tag="PLF77_03740"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPC08000.1"
                     /translation="MGKRTDIKKVLIIGSGPIVIGQACEFDYSGTQACKALRNLGYEI
                     VLVNSNPATIMTDPGMADVTYIEPLNIQALTEIIENEKPDAILPNLGGQTGLNLTSEL
                     YKKGVLEKHNVKVIGVQVDAIERGEDRIAFKETMNKLGIDMPKSEPATSVEEAEKIAT
                     KMGYPVVVRPAYTMGGTGGGIVYNLEELRLIASRGISASLIGQILIEESVIGWEELEL
                     EVVRDAKNQMITVCFIENVDAMGVHTGDSYCTAPMLTITPELQKRLQDYSYKIVEAIQ
                     VIGGTNVQFAHDPKTDRVVVIEINPRTSRSSALASKATGFPIALISSILASGMTLDEI
                     PYWRDGSLEKYTPSGDYVVVKFARWDFEKFPGSEDKLGTQMRAVGEVMSIGKNYKEAL
                     QKAIRSLEKKRYGLGFVKDFNAKSLDELMEMLSEPSSERQFIMYEALRKGASVETLSA
                     KTHIKKYFIEQMKDLVGLEERILQYKKKKMPDELLAQAKKDGFADKYLAQILGKEEEA
                     IRKQRQALGVTEAWEAVPVSGVENAAYYFSTYNAPDTVPVTGNRKVMVLGGGPNRIGQ
                     GIEFDYCCVHAAFALRDEGIESIMVNCNPETVSTDYDTSNKLYFEPLTVEDVLSIYEK
                     EKPEGVIVQFGGQTPLNIAAELAKAGVKIIGTSPETIDLAEDRDRFRMMMDKLGIPMP
                     KSGMASNLKQALKVAGDIGYPLMVRPSYVLGGRGMEIVHDEEMLKRYVNAAVGVTPER
                     PILIDKFLENAIEAEADAISDGTDAFVPAVMEHIELAGVHSGDSACVIPPVSIPLRHI
                     ETINDYTKKIAVEFGVIGLMNIQYAIANDVVYILEANPRASRTVPLVSKVCNVSMARL
                     ATQIMLGKKLKDLNLKNKTFRHFGVKEAVFPFNMYPEVDPVLGPEMRSTGEVLGLADS
                     FGLAYYKAQEATQQNLPSEGTVLITVEEKDKGGVLEAARAFKKIGFQILASEGTHRFL
                     KENGIEAEKILKMHEGRPNIVDAIKNGEIQLVINTPAGRLSKYDDSYIRKAAIKYKIP
                     YITTIAAAVAAVKGIAALKQGHGRAKSLQSYHAEIQ"
     gene            complement(13683..14087)
                     /gene="queF"
                     /locus_tag="PLF77_03745"
     CDS             complement(13683..14087)
                     /gene="queF"
                     /locus_tag="PLF77_03745"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HPC08001.1"
                     /translation="MPKEKKKNDFAGLTLLGKAAKPVRKLETFPNHNEKRAYVVTLQT
                     EEFTCVCPKTGQPDFAKIKIQYIPDKKIVESKSLKLYLWSFRNEGVFHEHVTNIILDD
                     LVAALKPRWCKVSAEFAVRGGIGITVDAEYKK"
     gene            14234..14416
                     /locus_tag="PLF77_03750"
     CDS             14234..14416
                     /locus_tag="PLF77_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08002.1"
                     /translation="MTENKQPLPENTWKCSNCGNTVVQITPPETCPVCKQKCEFLNVT
                     CYQPDCGFTGMDKRLK"
     gene            complement(14448..14798)
                     /locus_tag="PLF77_03755"
     CDS             complement(14448..14798)
                     /locus_tag="PLF77_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08003.1"
                     /translation="MNNGSTKMTDIKIDTNNLYKEEAFTDLTFATIRRLNPVNLDGSP
                     DASREPIFTGMTQLMSPNGPIPVQCLIEGAKTLAEAAEKLPVAIEKTVQAMIAEAKEM
                     EREEASKIVIPGQS"
     gene            15166..16962
                     /locus_tag="PLF77_03760"
     CDS             15166..16962
                     /locus_tag="PLF77_03760"
                     /inference="COORDINATES: protein motif:HMM:NF022783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3141 domain-containing protein"
                     /protein_id="HPC08004.1"
                     /translation="MNHYDLFKSWHQLMTQTTDMACNKKIKSEAPVEPLGALASWVDL
                     GTRLSHLTWARFSEDAWEYSIDFWQRCVLFMDTMRRRGNTMLEHYQQGMPPLLDYEFE
                     VVLDARKFPRPVNYSLLYIRHPEGKEPKPGKRPILVIDPRAGHGPGIGGFKKDSEVGM
                     GLAEGHPVYFASFTPEPLPDQTMEDVEQAEIRFLEEIKKRHPDRDFPMIYGNCQAGWA
                     VAMLGADRPDVAGPIVINGAPMSYWAGGDGINPMRLTGGLTGGTWATSFMCDLGGGLF
                     DGAYLVENFERLNPANTLWKKDYNLFSNIDTEQKRYLEFERWWTGFYLLNEEEILWIV
                     RNLFIGNKLEKGMLDIGHGHRIDMRNMKDPLVIFASSGDNITPPQQALHWISEVYPTT
                     EDLVKAGQRIVYLLHSHIGHLGIFVSASVARREHRAIIEHIEKMQKLKPGLYEMIIEG
                     ETGDHDPHQEQFRVRFEKREIKDVTCPIPKSAFDKMDRLSVANENLYLAFGRPFVKSL
                     IRHPQQAAALRWLHPARVSRYVWSDQVSPGMKIFDSWASWVKDNRSRAQESNTFSYIE
                     RRHSDNIATFLDACRDIRDTGLEVIFEAIYRE"
     gene            16989..18956
                     /locus_tag="PLF77_03765"
     CDS             16989..18956
                     /locus_tag="PLF77_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HPC08005.1"
                     /translation="MMNHPIKSRHPIASDLARRMKRGKIVSLDEAVGVIRDGDTLAFG
                     GFVGIGFAEGVARAIEKQFIAQGKPKDLTIIYSAGIGDGKDKGLNHLAHEGLVSRVIG
                     GHWGLVPKLQKLAIENKIEAYNLPQGIISHMFRDIAAHNPRTLSKVGLGTFVDPRHGG
                     GKINRKTRNDIVELLNFDGEDCLAYKTRPIQIAVLRGTTADLDGNVTMEREALTVDAL
                     SIAMAVRNSGGVVIVQVERIADRGTLRAREVRIPDILVDCVVVADPDDHWMTFAEKYN
                     PAYSGETRVPVTSLEPLPLDERKIIARRAALELKVNSVVNLGIGVPESIARIAAEEGL
                     LEYLTLTAEPGVIGGIPAGGLSFGAATNVEAIIDQPYQFDFYDGGGLDIAFLGLAQAD
                     RAGNLNVSRFGSRLAGAGGFINISQNAKKVVFAGTFVAGNLQVDVADEKLKIISDGDR
                     PKFIDAVDQITFSGAVGAQSGRTILYVTERCVFRLSEKGLMLVEIAPGVNLQKDILEK
                     MKFTPLMAEKLLMMDARIFRPEAMGLKEDLLTLPLAERFTYQPEENLFFVNFEGLSIR
                     STEQIDEIREHVERICRPLLPKKVQTIVNYDNFAILPELIEPYTAMVDHVVSRYYDKV
                     TRYTTSAFMRVKLGDLLAERSVAPHVFEKGK"
     gene            19106..19705
                     /locus_tag="PLF77_03770"
     CDS             19106..19705
                     /locus_tag="PLF77_03770"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HPC08006.1"
                     /translation="MIEKLRIIVSRIFVVLLFVLLCFSSSQWEEEAYFVTAVLFFIGV
                     ILVAIASLGRLWCSVYIAGYKTDHLITQGPYSMCRNPLYFFSILGALGIGFASETLLI
                     PLLIFIAFVIYYPSVIRSEEAGMMKLHKNEFAMYMQKVPRFFPKLSLLNEPEEYIVRP
                     KVLKRHIFDALWFIWIVGILEVIEALHELHIIPILFKIY"
     gene            complement(19867..21453)
                     /locus_tag="PLF77_03775"
     CDS             complement(19867..21453)
                     /locus_tag="PLF77_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HPC08007.1"
                     /translation="MNKQLQKEIERRRTFGIISHPDAGKTTLTEKLLLFGGAIQMAGA
                     VKARKASKHALSDWMAIEKDRGISVTSSVMKFEYADCDINLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDGAKGVETQTQKLMEVCRLRNTPIITFINKLDRDCLPPLEILSDIEDK
                     LQIECSPLSWPVGMGKRFKGVYNMFEKKLHLFTPGKARRSEEGITINDLSDPLLDELL
                     ESQADELREEVALLEGAANPFELKEYLKASQTPVFFGSAINNFGVKELLDAFVEIAPP
                     PAARPTTTRAVEPEEEDFSGFVFKIQANMDPAHRDRIAFLRICSGQFQRGMKVIHHRI
                     GKEVSLANATIFMAQDRTNIEEAYPGDIIGLHNHGTIKIGDTFTQKEPLKFTGIPHFA
                     PEHFCRVRLKDPLRMKHLQKGLMQLSEEGAIQVFRPLWGSDNILGAVGALQFDVTMSR
                     LKDEYSVYADYEKTDYAAARWVTCDDAKIMEDFQKKNQTNLALDTEGNLVYLALSEWR
                     LGHTMEEWPKVQFHKTMEKS"
     gene            complement(21516..22493)
                     /locus_tag="PLF77_03780"
     CDS             complement(21516..22493)
                     /locus_tag="PLF77_03780"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC08008.1"
                     /translation="MISKIEKDFYQITLRMPYRLRHVNAYLFIYEGKLALFDTGLNTP
                     QAFETLGNDLSGIGFGIEDIQHIYLTHVHSDHCSMAGVLQKRSGAQIHLAQAAFEEYR
                     HFREAGPTIKQLRIYYARQGMSQEQIGLVIEEYENIRGIIADFDAADFLRDKDVREFG
                     NWKIEIISTPGHAAGHVCFFCREMKFLLSGDHVLPYIAPVLSPDIFDEAFRPLAAYLD
                     SLNALKNLCVTAVYPGHGISFVDLQERIADIREHHSKRKTMVLDRVHAQPRTARDISH
                     ETLAPTLSDFDKFLALNETIAYLKELTAEGAIKEDTLNDVLVYVRPSGY"
     gene            complement(22518..24335)
                     /gene="typA"
                     /locus_tag="PLF77_03785"
     CDS             complement(22518..24335)
                     /gene="typA"
                     /locus_tag="PLF77_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HPC08009.1"
                     /translation="MKKFHLRNIAIISHVDHGKTTLLNAMLKQTGVFHANRVVEDRVM
                     DSMDLERERGITIMAKNTAVNYNDVKINIVDTPGHADFGGEVERSLNMVDGAILLVDA
                     SEGPLPQTRFVLKKALGLRLPIILVINKIDRSDARIEEVISETYDLFIDLGAEEHQIE
                     FPILYTNAKAGISHEKIGDDSADLQPLLQTIIEYIPPPQGDDEAITQFLVTNLDYDPY
                     VGQIAVGRLQSGKLEMNTNYSLCTEYKIIPGVKLTALYTFFGLAKKPAQSVESGDIIA
                     LSGVENVKIGDTISSLAEPRPLPRLLVDEPTVSMMFYVNDSPFGGKEGKYLTSRHLLE
                     RLEKEQLRNVAIKIKKLERTDAFEVCGRGELQMAVLIETMRREGYELMVSRPQVITRQ
                     VNGKTCEPVERLFLDIPEEFVGKITEKLSIRKGRMESLVNKGSGRVSMEFLIPSRGLI
                     GFRNQFLTDTKGGGVMNSLLEDYAPWFGSIPQRTTGVLVADRSGRVTPYASYAMEDRG
                     EMIVEIGTDVYAGMIVGERNRSSDLNVNIIKEKKLTNMRASTSDATVILRPPRILSLD
                     QAIEFIAEDELVEVTPKSVRLRKMELDATKRQMKSRREE"
     gene            24533..25426
                     /locus_tag="PLF77_03790"
     CDS             24533..25426
                     /locus_tag="PLF77_03790"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPC08010.1"
                     /translation="MLNFLPSKLVGAIAGLLLVVNVLFWITILFIFTLPKILIPWPSL
                     LKVINKILQWIGENWIACNSGWMWLTQKTRWDVQGAENLNHEGWYMVVSNHQSWVDIL
                     VMQHLLNRKIPLLKFFIKRELIKVPLMGFAWWALDFPFLYRHSSEYLKQHPEQAGKDF
                     EATRKACEKFATIPTSVMNFLEGTRFTPAKHDKQKSEYKYLLRPKSGGLALALNVLGE
                     KFSSMLDITIVYPDGIPGFGDFLCGRVKRITVRMRTIEIPKTLLQGDYENDQAFRDSM
                     HKWVQKLWREKDKQIQALMKK"
     gene            25645..26592
                     /locus_tag="PLF77_03795"
     CDS             25645..26592
                     /locus_tag="PLF77_03795"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPC08011.1"
                     /translation="MSKTLGKIFTILAVVILAGVFNANAAEEARDGRFIAYDDGTVWD
                     SQSNLLWASHDNGSNINWSDAEEYCKTYDGSGYKGWRLPTKDELLTLYDRSIFGNNGY
                     RLTKLITLSGSYPWTSDVQGSEALVVGFDKDRQMWFPESVNHKSRNRVLPVRFGKEGK
                     MTVAEPKQEEVPPLPPPPPPETIAPPPPPPPPVQEKVSISLNIEFDTAKSFIKKKYHN
                     NIKQVADFMQTYPDTNVVIEGHTDNVDRFNNPENNIKLSQARADSVRKYLIDKFGIDE
                     SRITAVGYGPSRPIADNNTPEGRMKNRRVEAVIEKMVTK"
     gene            26840..27421
                     /locus_tag="PLF77_03800"
     CDS             26840..27421
                     /locus_tag="PLF77_03800"
                     /inference="COORDINATES: protein motif:HMM:NF037007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC08012.1"
                     /translation="MAAARTVFAQKGYNGARIDEIAARANVNKATIYYQIGDKDTLYT
                     GALHQVIGHVARDIADAVSRKVHPEEKMKAYITFIAGAVEANPDLPPMMMREIASGGA
                     TLPRLVIEDIASVMSILAGILEAGKEQGIFIETSPFLIHMMIMGTILFFKKGSPIKDK
                     QSWLPEALLAYDQKISANLGEEVASLVLKAIKQ"
     gene            27449..28459
                     /locus_tag="PLF77_03805"
     CDS             27449..28459
                     /locus_tag="PLF77_03805"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPC08013.1"
                     /translation="MKKRIIFVVFIIFFLIVGALVYFGQKSNREKELVYSGTIDAIQA
                     NLSFQVAGRVSQVFVQEGQSVSQNQMIAELDRSEFESRFEQARANLERAQKTRRQLET
                     VLELSRKTLPAEVARAKAGVQSAKDSLKDAEKNYRRFEELFGKNVVSEKERDALRLQY
                     DVAQSRLAESESVLKLAQGNLTRIDAVKQDIDVAAAQINAAAASLNQAEIQLNYTQLK
                     SPANGVVTSRNIEPGETVTPGREVITVSDLSRVDLKIFVDETEIGKVKPGQKVDVQVD
                     TFPGKTYTGYVSFISPEGEFTPKVIQTTKERVKLVYLVKVSIGNPHLELKAGMPADAW
                     LK"
     gene            28472..29245
                     /locus_tag="PLF77_03810"
     CDS             28472..29245
                     /locus_tag="PLF77_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC08014.1"
                     /translation="MTKAPESPPSMVDVRNVTMRFKSVEAVKDISLQIPKGHVLGLVG
                     SDGAGKSTLLRLVATMIQPTSGNIYIDGLHVVTDKQKIKNIIGYMPQRFGLYQDLTVE
                     ENMDFFMDIFAVPRRERKERKEKYLGFSNLLPFTDRMAGNLSGGMKQKLGLACVLVHE
                     PQLLILDEPTNGVDPVSRSEFWEILENMQKEGMTIMVSTAYLDEGEKCHRLGLMHNAM
                     LLDLAPPAVIRGGYPTLEEAIIERITQADGALAHDTFQL"
     gene            29226..29981
                     /locus_tag="PLF77_03815"
     CDS             29226..29981
                     /locus_tag="PLF77_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC08015.1"
                     /translation="MTPFNSDSISVRHLEKRFGNFVAVNKISFSVKKGEIFGFLGSNG
                     SGKSTTIRMLCGIITPTSGSGTVAGHDIVTEAEDVKQSIGYMSQKFSLYEDLTPFENL
                     RFYLGVYNVPPPQWRERIDWVLNMTRLQDARDRKTRELPPGWRQRLALGCSLLHKPDI
                     LFLDEPTSGVDPVTRRHFWSFIRKLAEGGMTVFVTTHYMDEARFCERIVMINEGLIVA
                     AGTPADIIAGACPGAADADLTDAFIKIMKRDKA"
     gene            29978..31111
                     /locus_tag="PLF77_03820"
     CDS             29978..31111
                     /locus_tag="PLF77_03820"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC08016.1"
                     /translation="MKFIRLKAIIRKEFYHLIRDYRSLYLAFAIPLILIILFGYALSL
                     DVDNVETVFIDYDHSDLSRDFIRKLDASSYFHVSGMLPSTKDVVRYLDEDRARLAIVI
                     PPDFKKNIGADRETPLQIIIDGSDPTFGNIMRGYISAFVEQYNRKLLMDFLNRQGQEH
                     INPPVDGRIRIWFNEDMESRNFIIPGIVAVIIMIVGALLTSLVIAREYENGTMETLKS
                     LPVRAGELLLGKALPYFFIGLTDVLIAILLSQLLFGIVMKGSFWLMILSSSLYLGVAL
                     SLGLLISNVTQSQLLANQIAIMATYLPSFLLSDFVFPIVNMPAVIQAITFITPARYFI
                     DILSGIYLRNLGFAYLWPSMLVLLLMFIVLTSMNYRLLRKEGL"
     gene            31108..32229
                     /locus_tag="PLF77_03825"
     CDS             31108..32229
                     /locus_tag="PLF77_03825"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC08017.1"
                     /translation="MNWLRIRELVRKEFIQLFSDKRNRALMVVFPFIMMLVFGYVVNY
                     DIRNVRMAVLDYSKTHESRSLIKSFEGGNIFHVTHNVIHDRQMTELLLRQKIDMGIKI
                     SHDFASVIRAGQTAPVQLLVDGSMSNMTSIRVAYTATILEKFNREELRRLYPMDMRYG
                     RVDARIRTWYNPNLDSQFFFVPGIVAFLIMLTSFIFTSIAIIREKEDGTMEQLIVTPI
                     RSYEFIIGKTIPYILIALLQLFAVTGVAVYWFEIPFKGSFLLLLLAACLFLLSTLGIG
                     LFISTISSTKQQAMMTAFFFVVPFFMLSGFVFPIANMPDPVQWMTYLNPLRFFLVIIR
                     NIFLKGVGFDVLWPQYLALTILGAIVFTGAIARSKKRLD"
     gene            32320..33312
                     /locus_tag="PLF77_03830"
     CDS             32320..33312
                     /locus_tag="PLF77_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08018.1"
                     /translation="MLKVVADQKAIKKYAGLFNKKFKPFVAETIKVKLGHQGASFPAR
                     VLWLQELGIWKFSQAVKEVRYWNAFGLDKPGAASVLSIASEINFPWNQIDRKMGGAFA
                     EDHWGNVFVIHRGKIGGGKKGIGKSLFEQNYRGVWSFMEDGDTISQVAVIGNLKSERF
                     ASQTALFVKKIEMMKLSAAESAQTEMDFAEISFREDLIGSESPLPEDEIIFACDHDLV
                     VSRLAAELQRLKFKTGNDSESELFAVDTSGNRISHIFDVVTDAKEKNVMAAAGRLMLQ
                     TSAGALNPLPVLVLPDDIMNHYKSDLRRIHICVIGFHWQDTQAVFSGLEKISIR"
     gene            complement(33397..35451)
                     /locus_tag="PLF77_03835"
     CDS             complement(33397..35451)
                     /locus_tag="PLF77_03835"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HPC08019.1"
                     /translation="MKQASDKNQTDPAITTSKPERRRILIWGILCTLCFAVIFAFKPN
                     LMQRLDYLEYDFLLAKFPNNNASSKLVIVDLDEKSLSRYGQWPWPRYRVAGLLDKVIA
                     MQPDIISLDIVFAEPDRTSAGPLLKDLGDVYRIKIDLHGLPRSLKDNDQILAQTLAGG
                     PFVLGTIFHFDELKKGSDRCVLHPVKSSYINDDVNQNAHTEIPVSTGVLCNLPMLSDK
                     VSSSGFFNFSPDSDGVLRRLPMLIQYHGQLYPNLALATVLKLKNENHVILKKDRNHLQ
                     SIHYRGNAVPVDRHGQMLIKFRSSAQKYEYVSAADILDNNVSPERLRGRIVFVGTSAV
                     GLKELLNTPLGPVFPGVEVHATAVDNLLKGDFISLPGWSNGFILLLVMVSGLAMSLLI
                     SYQKAATGFVAMLLFAGGLWMTTHQVFFRMGFFVATAFPIASVICNYMFLTALKYRME
                     EKKNLAHMQELLLTQDITIESLANLTEHRHQETGGHIKRTRLYVRLLADHMKHRDKYR
                     TFLNDENIEMLDKSAPLHDIGKVGIPDKILLKPGKLTGEEFEIIKTHTTNGRNLIRSS
                     VRQFGKISFLNIAEEIAYTHHEKWDGSGYPQGLKGDAIPISGRLMALADVYDALISKR
                     VYKEAYPHAEAVEIIVNAKGKHFDPDMVDAFLEIHEQFRNIAREHADSQEAKESPKPD
                     AA"
     gene            complement(35474..36076)
                     /locus_tag="PLF77_03840"
     CDS             complement(35474..36076)
                     /locus_tag="PLF77_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPC08020.1"
                     /translation="MKQLIGTVFCLGLVLVLAGCAVRSTVVLIPDPDGHVGQLVVANE
                     GGQQILNEKNQSVRVADLKTAPGKVKTMSAEEIQDTFADALAAEPLPPVKFILHFMPD
                     SDQLTDESRAVLPDIFQTIQSRQSTDIVISGHTDTVGEKNYNYTLSLERAQAIFKILE
                     AQGAVPAHITVTSHGEGNPLIKTADEVAEPKNRRVEVTIR"
     gene            complement(36156..36605)
                     /locus_tag="PLF77_03845"
     CDS             complement(36156..36605)
                     /locus_tag="PLF77_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HPC08021.1"
                     /translation="MKRILILCLAMMFLLPCIAMPEESPVGKTKTSKGSVFVVRDGRQ
                     MPVAVGDRLFQNDTIRTGASSSVGIIFEDNTILSLGPDSEIVIDQYVFAPEKGVFSMI
                     ARMVRGTASYLSGIIGHQSPESVKFRTPEATIGIRGTHFLVKVDGCP"
     gene            37119..>38164
                     /gene="cimA"
                     /locus_tag="PLF77_03850"
     CDS             37119..>38164
                     /gene="cimA"
                     /locus_tag="PLF77_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HPC08022.1"
                     /translation="MKNKILVYDTTLRDGTQGEQTNFSAEEKLRIAQRLDDVHFHYIE
                     GGWPGSNPKDARFFEMAKNAAFKNSRLTAFGSTRRAHTKTESCPNLKALIKAGTPACA
                     IVGKSWDLHVTDILKTDLNENLAMIADSIAYLKSMDKEVLFDAEHFFDGYKNNSRYAT
                     RVVKTALAAGADFIVLCDTNGGCLPHEICALVKRISAHVPFEKMAIHVHNDGGLAVAN
                     TLAAVQKGVAMVQGTVNGYGERCGNADLIAVIANLQLKMGKKCLPPESIKQLTNLSHY
                     ISDVANVPPLNSRPFVGRSAFAHKGGVHVSAVVKNSAAYEHIEPELVGNHRRVLMSDL
                     AGKSNVEYKAKELG"
BASE COUNT         9404 a         9614 c         9481 g         9565 t
ORIGIN      
        1 tggctgattt tctgacaccg gctattttga tctgatgacg aggcggtttt tgttcaatga
       61 ttaaaaaaaa atcttatgtc tttgtatgga ttgcccttat tctaaccatt gccggttgcg
      121 gcggtttgca cttttctcaa ctggcgccgg aggccatcga ctttcatccg cagagtattg
      181 ctgtttttcc gattgaagtc tggaatcaca aagaacccga taacagaacg gtggtccaac
      241 agattgtggc cggctcactg attgccaaga aattgtttac ttctgtcaca gacgcggaaa
      301 aactgcaaaa acagatgcaa aatgatgaac tattgaaaaa aacagcggaa gagtattttt
      361 ccaaactgca acttctgaag ttttccgatg ctgatttaag taggaaaatc ggtgacatcc
      421 tgcaggttga cggtattctg ctgttatccg ttgacgaatg gaaatatttc gttgaggcag
      481 ataagaaaat ggcgcaggtc ggtttgacaa tggagctgta tgatgttacg aacggcaaat
      541 taatgtggaa agccaatcac accatcaaga aggattatgt attaataaaa ccgggcttgc
      601 ctgatatggc ccgggatgtc gcaaacaaaa tggtttatta catgccgcat tgaaaagaag
      661 gacttgaaaa ataagaaaaa acggaatagg gagaacctcc atgaaagaag acgtacagaa
      721 agccattgat accatcaggc ccagtctgca ggcggacggc ggcgatgtgg aactggtcga
      781 tgtcagcgat gacggcacag tcaaggtcag gttaaccggc gcctgccacg gatgccccat
      841 ggcgcagatg acgttaaaaa tgggaataga aaaagtcatt aagaaactgg tccccggagt
      901 taaagagatc gtggccgtct gagccgtttc gcacatcagc aacgggaaag tggcttttta
      961 agaacaatcg tttcctgaca agacaaagcg ggaaaggtgt attttaaaat aacgataaaa
     1021 ggagcataaa attatggcat atgtgattac tgacgaatgt atagcttgcg gttcttgtga
     1081 aagcgaatgt ccggaaggag ccatctcaga gggcgatgac aagtatgtta tcgatcctaa
     1141 actgtgcaat gactgcggaa cctgcgccga ccagtgtccg gtagaagcga tcgttcccgg
     1201 agaggggaaa taattcacag acgaaaaatc agctccggga ataaatctgc ttttacgcat
     1261 tgattcattc ccggttttat tttttaaaac acggttaccg gttcattgcg ccgttgcgca
     1321 ggtgaaagcg gagtcacatg atcaaggcaa aaaaatttat cacctttgag ggtatcgaag
     1381 gttcgggaaa atcgacgcag atcaagctcg tcgccgaata tctgcaaaac cagggcgttg
     1441 aactgattgt cacgcaagaa ccttccggaa cggacatcgg gcgacagatc gccggcattc
     1501 ttttccaccg caggcacaat catctgtgcg cagaagcaga aatgttttta ttctgcgccg
     1561 cccgggccca acatgtccgg gacgtcattc tacccgccct ccggcagggg acactcgtgt
     1621 tatgcgatcg cttttccgac gccacctacg cctatcaggg tttcggacgc ggcctcgatc
     1681 agaaattcat cggcgcggtc aatgattatt ccgcgatgca gttgaaaccg gacatgacct
     1741 tattgtttga cctgccggtg gaaaccggac tggaaagggc aacggcgcgc aacaatcgac
     1801 tcgccgaccc ggccgccacc gaccggtttg aaagggagac tctggatttt caccgtcgca
     1861 tccgtgaagg gtatctgact attttaaata atgaaccgga acgtttccgc ctgattgacg
     1921 ccacccggaa gattccggtc attcaggaag aggtgcgtcg tcacataagc agttttatca
     1981 aggtctaaaa tcatcttttt aaaagaattt tttcaggcat tatgtcgctc ggcaatattt
     2041 acggtcacga aaaaaaaatt gacatgctcc gcaaaaccct cgcccgccgg cgaatcgcgc
     2101 acgcctatct cttcagcggt ccttcggcga ccggcaagaa aacgctggct gtggaattcg
     2161 tcaaggccgt caattgcata agttcggatg accggcatga tgcctgcgat gcctgtccgt
     2221 cctgccgcaa aattcaacat gccagtcatc cggatatttt ttttgtggaa cccgagggcc
     2281 agttcatccg catcgcttcc atccgtcaga tccaggagcg aaccacctgc cggccgctgg
     2341 aggccaaaaa aagagctttt attattgatg atgccgatag aatgaatgag gaatccgcca
     2401 atgccctttt gaaaatgctg gaagaaccgt cgccggccaa tatgctgatt ctgatcacaa
     2461 cgaggccgca cgctatgctt cagaccatca tttcccgctg ccagcacatc cactttaatc
     2521 cgctggccgt cgaaacggtg gcgcggtttc tcgtatccga aaaaaacatg gagcattcaa
     2581 gggcaatgct gctggccgcg ttatccggcg gaacaatcgg caacgcgctg gagctcaacc
     2641 aagaggaagt tgtcgcgtac cggaccgaac tgttcaacac gctggccctg accgaccgga
     2701 aagagccttt cagcctgatc cattttgcct cttttctggg ccagaaaaaa aaggaaatcc
     2761 ggcagagcct gaatattctg aacacgttct ttcgcgatgt gctggttttc aaggaagtcg
     2821 gccagaaagg aatgctgatc aatcaggaca atacgcctct gattgcggcc tgcgcggcca
     2881 gattgaccgg aaagcagatt ctgcaaaaca ttgcgcagat agaaaaagcg ggggacatca
     2941 tcgaacaaaa cgtcaacaaa tcattgacat tggaaacaat ggcatttaag ctgaattatt
     3001 aaaaaagggt ggatggacat tgcttgtaaa tatcattgga gttaaattca agaaagaagg
     3061 ccggatctat aactttgacg ccgccgatct tatcctgcac cgggatgaac aggtgcttgt
     3121 cgaaaccgac agcggcctgg cgctgggcac tgtcgtcacg gatgtgaagc gatgcgagcc
     3181 gtcggccgtt ccggccaatc tcaaaaaaat aatccgcaag gtgacggacg acgatttaaa
     3241 gatcagaaaa gagaatgacg cgctggaaca ggaggcctgg aaattctgtc tggaaagaat
     3301 tcgcaaacgc gagctttcca tgaaactggt gaatgtcgac tgcctttttg aaaaaaacaa
     3361 aattattttt tactttacag cggacagcag ggtggatttt cgggaacttg tcaaggatct
     3421 ggtccagaag ttcaaaacga gaatcgagct caaacagatc ggcgcccgtc aggaaacgcg
     3481 gattaccaaa ggactgggaa tttgcggccg gatggtgtgc tgtgccggca tcctgcaaaa
     3541 tctcgaacga gtcacggtca agatggccaa ggaacaaagc atgtcgctga atcctgaaaa
     3601 aatttcaggc ctgtgcggaa ggctgatgtg ctgcctggcc ttcgaatacg acggctatgc
     3661 cggaaagaaa aaagccgcca aagcggccaa aacgtccggc agcgccactg aagcacagga
     3721 agaaaagcaa acgctggatc tggaaaagat ggatgacatt gatcatcttc agtcatgaat
     3781 ggagaaacgt cacatatgcc gaaatcattt tatatcacga caccgattta ctatgttaat
     3841 gcttcaccgc atatcgggca tgcttatacg accattgtcg ccgatgtgct ggcccgatat
     3901 caccgcatga tcggagataa aacgtttttc gttaccggaa ccgacgaaca cggcgataaa
     3961 atcgccgagg cggcgcagaa agccggatta accccgaaac aatacgcgga taacatcagc
     4021 gcccagtttc gcgccctttg tccgcaactt gccatctcga atgattattt catcagaacc
     4081 accgacgcca atcacatcga aaccgtgcgc tatattcttc agaaggtgta cgatgccggc
     4141 gatatttatt tcgggagtta cggcggttac tactgtttcg gctgcgagcg atttctcacc
     4201 gaaaaggaaa tcgtcgacgg taaatgtccg gaacacggcc gggaacctca gtatattgaa
     4261 gagaaaaatt attttttcaa aatgagcaaa tatcaggact ggctcatcga ccatatcaaa
     4321 aagaaccccg aattcatccg gccggagaga tacagaaatg aagcgctggc ttttttacgc
     4381 gatcccctgg aagacctttg catttcccgc cccaaaacca gactggaatg ggggattacc
     4441 ctgccgtttg atgagcagta tgttacctat gtctggttcg atgccctgat taattacgtc
     4501 accgccgtgg ggtatcccga cggggagcat ttcaagacct tctggccggt cgccgaacac
     4561 ctcatcgcca aggatatctt aaaaccgcac ggcatctact ggccgacgat gctgaaagcg
     4621 gcgggtattg aaccttatca gcacctgaac gtgcacgggt actggaactc cgatcagagc
     4681 aaaatgtcca aaagcctcgg caatatcgtc cgcccgctgg atttgaagga taaatacggt
     4741 ctggatgcgt ttcgatattt tcttttaagg gacatggttt tcggtctgga ttccaatttc
     4801 tccgaggatg cttttgtcgg tcgcctgaat tccgatctgg ccaacgatct gggaaacctg
     4861 gtcagtcgcg ccattaccat ggccatcaaa tattgcggcg gcagagtgcc ggccgttgca
     4921 ggcgatgaaa aaaatccggc ccttcgcgag gccgctttga aagccgtctt agacgttgag
     4981 gcgagtttca gggatatgtc tctgcacaaa gccctgattg ccgtctggga tttcattaat
     5041 atcaccaaca aatacatcgt cgaaaacgag ccttggtcgc tggccaaaga cccggccaac
     5101 ggtaaaaaat taacggcggt catgtatgat ctgctttcgg cgctgcacgc gatcgccgtc
     5161 atgctttcgc ctttcatgcc gcaaacggcg gaaaagattc tccgacagat cggatgcgaa
     5221 aaaaccgaag gtctgaaact ggacgacatc aaaacggaca acgcattgaa aacaggccag
     5281 gtcctgaccc gcagcgatgc cctgttcccg cgtattgttc agcaaaaaga aaaacccgag
     5341 gatcagaaag cagccatgac cgacctcaag ccggaaatag attatgaaac attttcaaaa
     5401 gtggatttac gcgccgcaac gattctcgcg gctcacgccg tacccaaatc caacaaactt
     5461 gtaaaactga cggtggacat cggcgaagaa cgcaccgtcg tggccggcat cggcaaggat
     5521 tacaaaccgg aagaccttat cggaaaacaa atcgtgattg tggcgaattt aaaaccggca
     5581 aagttgatgg gggtagaatc ccgaggcatg ctgctggcca ccgacacgcc agaaggatta
     5641 acccttctga cgttcgaccg cgcggcaaaa acaggaggaa aaatccgcta gccgctctcc
     5701 cgcagcaaga gcggccggcc ttatgaaaat cagtctttga gaagtttctc gatcagcgca
     5761 acatcaaatc cgtccacaac ctggccttta atgtagaata aaggcgttcc ccgcagtccg
     5821 acctgcgacg caagctgcac atgcgtcttt attttttcat cgattttggc atccgcgcac
     5881 acattcagac tggcgttatt gtcgagcttg cctttcatga catcgtcata tgtttttaaa
     5941 acgtccttgg aacaataaat atgtttgatt tgatctgcgg attctttgga cagcgggaaa
     6001 aagaaaacat gcatggtcac atctttgcgc tgggaaaaga actgataggc ctgacggcaa
     6061 tggtaacaat ggggatccat aaattcaaca atgatttttt ttccgttacc gatcttgaga
     6121 gcgttctcca gaggcaattt ctccatcttc tgcgccatct tctttaaaag actatcacgg
     6181 gtgatgttga cgccgctttt ggacaccagg gtgccgtaaa gcacgatctc gtccttcggc
     6241 atgtaatagt aaagctgatt gccgctatac acttcataaa ttcccttgat ggatgtgggt
     6301 acgatacttt caaacttgtt tttcggaaaa ctttttttga actggtcctc caccgatgcg
     6361 tttttggcaa atatcacaac cgggatcaga caaagcacaa acaaaataat ggccgtaagt
     6421 tttttcacag ggaacctcct tcataaatca tgggatatta taaaacaatc catcgatccg
     6481 tcaagcgtat ccgtggaatc gccgtttttt caatttgtat ccggtacgta tatatcatct
     6541 taagacattt gataattttt gtcccgaaat aatctcaggc aggctttgac cacctcggcg
     6601 tcatagagag tgcccgcatt atcttcaatt tccgcaagcg ccatttcaag cgccagcgcc
     6661 ggtctgtagg gacggtacga tatcatggcc tccaccacat cggcaacggc gagaatacgg
     6721 gactcgatga gaatttcgtc attcttcagt cccaaaggat atcccgaacc atccatgcgt
     6781 tcatggtgtt gataaacaat atcggccaaa ggccagggcg attcaacttc tttcatgatg
     6841 tcataactgt agcggggatg atttttcaca aaagaaaatt cgatatcact gagaatcgcg
     6901 ggtttacaga gaatttctgc cggaaccgaa attttcccga tatcatgaat ggcgctggcc
     6961 attcgtatcg cctcaatttt atcctgagga agtttcattt ccgtggcaat agcacgggcc
     7021 aaatcggcaa cccgtctttg atggccggcc atgtaaggat cttttgcctc tgatgccgtc
     7081 cccaggacct gaatggttat tttgatggat tgacgcagac tttccagcgt ttcattcagt
     7141 ctgttttccg ctcgtctgcg aagcgttata tcctcataaa tcacctggga ctgtttctgg
     7201 ccgttccaga aaatttcctt gcggaaaaca tgcagatggc ggatttcnnn nnnnnnnnnn
     7261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7321 nnnnnnnnnn nnnnnnnnnn nnnnnnngtt tctggccgtt ccagaaaatt tccttgcgga
     7381 aaacatgcag atggcggatt tctccgtcct ttctgataat gctgatttca tattcggacg
     7441 gaccgaactg gcccttcagc cgtttcttct ttctttcttt gaacgcttcg aacgtctcag
     7501 gcgtatagcg ctcctgcagc ggcgtgttct gcaattcctc tttgctgtca tacccgtaca
     7561 tgtccaggat agccctgttg gcataaagcg tttttccttc aaccgtcgaa atacggacac
     7621 cgagcggaga gtcatcgaga gaatgacgga aattttcttc gcttttccgc agagcatctt
     7681 ccatttgctt gcgttcatta atattcctgc tgacccccag aagggacaac ggtttgcctt
     7741 tgtcatctct gattaaactg aaattgcatt ctccccagac ggtttggccg tttttacagc
     7801 aaaactcaag ttccagagac cgctccaaaa cataatccgg aggagctccc agagctttag
     7861 gcatttcaat ggcaaagaaa tccatcgccg cctggaaaga ttgtacggtc aaaaatttgt
     7921 cgagagagaa ttttttgaag tcttcaagcg aataacccat taacctttca acagaggggc
     7981 tgatataatt gattttcagg ttcagatcca tcagccacac gtaatctttc atattatccg
     8041 ccagcagacg atattttgct tcactttgcc tgatgagttc ctccgaccgt tttctcgcgg
     8101 tgatgtcgtg catattgaca atggcggctt ccacgacatg atccttgacc aaattgctgg
     8161 ccgtgacttc aaacgtccgc cagcttccgt ttttatgatg caaccgtatt tcgcctttct
     8221 gcacaggcgc ttggatattg tggaaaagtt tccccgcagc atcagcgacg atttgcgcat
     8281 catccggatg aaccaggtca tatcccgatg cccccagcct ttcttcaaca cggtatccga
     8341 gtatcttttc aacagccgga ttttcgtaag tgatccttcc tcgcgtgtcg gcaataataa
     8401 tgatgtcgga cgattgttcc gtaacagatt tgaataactg cctttcgcgg tgcagctttt
     8461 cctgcacctg tttgcgttcg gtaatatccc ggacaattcc tctgaaacct atcggggtgc
     8521 ctgcaacatc cttcagcaga aagacaaaac cttcgatatc tcttttggcg ccgtcttttc
     8581 ttatgatctg ccattccaga tttgttaccg gctgatccgt ttcgtaaata cgattaaaaa
     8641 caatataaag cgcttttgaa tttttttcgt ccatatactg acggtttttc attcccgtca
     8701 tttcctcgcg ggaatagccg aaatgccggc aggtggcatc gttgaaaaaa gtaaaattgc
     8761 cggaaaggtc gacttcaaaa tacccttcct ccatgttctc aagaatggtc cggtattttt
     8821 cctcgctccg gcgcagcgat tcttccgctt ttttgcgctc cgtaatgtca atccccatac
     8881 ccaccacaaa aggcttcccg tcgtgatgca gaagattgcc cgaaagcaaa tacggtgtta
     8941 cagaaccgtt tttggaaaga accggcattt caaaggcgat atggcctgtt ctttgaaatt
     9001 cctcgaactt ggccaataaa aaatctttct ggtgttccga tatcaaagcc gacggtctca
     9061 tttggccgag ttcctcatcc gaatatccgg aaacagcgga aaaggttttg ttccatctga
     9121 agaaagtcat actggcggta tcaaacatga agaaagggac cgtcagactg tccagtacgg
     9181 aacggttgaa atcgatttct ttacaaacct gctcttccgc ccgtttgcgt tcggcaatgt
     9241 cgcggacggt tccgatgatt cctatcgcat tattgtcttg atctcttaag aacgttatct
     9301 tgttttccac ccatatcgtt gatccatcct tgcggatatg ctgatattca agaacccgcg
     9361 atcttttcaa atcccggtcg ggccgctttt ctatttccat ctcctcagcc agcatctcca
     9421 tgaaccaggc gagcgtttca ggatcaacaa gattctccac tttcatggtc atcaattctt
     9481 ctgccgtgta tccaagaaga tgatacgtgg aaggactggc atacgtataa ttgaaattca
     9541 tgtcgatggt gaagatgacg tcggtcagat tttctgccag cagacggtac ttggcctcac
     9601 ttttctcaaa ctgttctttg atcttctttt tatcggtgac atcctgggtc tgtgtgagaa
     9661 catacaactc ctgtccgatg gtcaccagtg cggcggccac atttccccag tgaacgttgc
     9721 cgtttctgtc acgaaaccgc aattgaaaat ccgtcacggt gcctttttca agaagttccc
     9781 gaaccagtgc gtcgcgttcg gccatatcca cccagagatt gatttccgaa gaggtgcgtc
     9841 cgatgagttc ttcgcggcgg tatcccagaa aattactgta aacctcgttg acttcaacat
     9901 agcggccatc ctgcaggctg gatatgcaga tcccgatcgg actatatttg aacgccagcg
     9961 cgtatttttc ttcagggccg caaaaagact ccttcccgtt tttatgtccg gccgtctttg
    10021 gggacgcgcc gctgatcgcc ggtttgtctt tctgcggcat cctgcgggta tcctgcgaaa
    10081 ccttcctctt taatccggaa gggttgacct ctctggactt ggattttttt cccaacgtct
    10141 tggtactgtc agggagagag agtaaggcgt taacgttttt atcttcttta ccgtccttgc
    10201 tttccattcc ttctcctcac gcaaagtcgt ctatacagcc tcaatgtccg ctcaaaattc
    10261 tatcggattg ttataatgtg aatgaagcta aaggaagaat tttaataagt caatgtaaat
    10321 atacggaaga tattcgcccg tgtctcatta aaagtttgcc cacagctttt ttataaacaa
    10381 tgttctttta tatattatta acctataatg agctgtcgaa aaataaaagc tttattttct
    10441 caaatcggag cctgattcct actgaatttc cgcgtgatag ctctgcaggg attttgcccg
    10501 tccgtggccc tgcttcagcg ctgcaattcc cttgacggcc gcgaccgctg ccgcgatggt
    10561 cgtgatataa ggaattttat atttaatggc tgctttacgg atataggaat catcatattt
    10621 gctgaggcgc ccggccggtg tgttaatcac cagttgaatt tcgccgttct tgatggcatc
    10681 cacaatattg ggcctgccct catgcatttt aagaatcttt tcggcctcga ttccgttttc
    10741 tttcagaaac ctgtgcgtcc cctccgaggc aagaatttga aaaccgattt ttttaaacgc
    10801 ccgcgccgct tccagcacgc cgcccttgtc tttttcttcc accgtaatca gaacggttcc
    10861 ctccgaggga aggttttgct gagtcgcttc ctgtgccttg taataagcca ggccgaaaga
    10921 atcggccagg cccagcactt cgccggtgga ccgcatttcc gggcccagaa ccgggtcgac
    10981 ttccggatac atgttaaagg gaaaaacggc ctccttcacg ccgaagtgac ggaacgtttt
    11041 gtttttcaga ttcagatcct taagcttttt gcccagcatg atttgcgtgg ccagacgcgc
    11101 catggacaca ttgcagactt tggaaaccag tggcaccgtg cgcgaggcgc ggggattcgc
    11161 ctccagaatg taaaccacat cattggcgat ggcgtactgt atgttcatca ggccgattac
    11221 tccgaattcc acggcaattt tttttgtata gtcattgata gtttcaatgt gacgcagcgg
    11281 aatgctcacc ggcggaatga cacaggcgga atcgccggaa tgaacaccgg ccagctcgat
    11341 atgttccatg accgccggaa caaaagcatc cgtgccgtcc gaaatcgcgt cggcttccgc
    11401 ttcgatggcg ttttccagaa acttgtcgat cagaatcggc cgctccggcg tgacgccgac
    11461 tgccgcgttg acatagcgtt tgagcatttc ctcatcatgt acgatctcca tgccgcgccc
    11521 gcccagcacg taggagggac gcaccatcag cggataaccg atgtcaccgg ccactttcag
    11581 cgcctgcttg agattgctgg ccatgccgga tttcggcatg ggaatgccca gtttatccat
    11641 catcatgcgg aaacggtcgc ggtcttccgc cagatcgatc gtctccggcg atgttccgat
    11701 aattttcaca ccggctttgg ccagttccgc cgcgatgtta agcggggtct gtccgccgaa
    11761 ctgcacaatc accccttccg gtttttcttt ctcgtagatg cttaaaacat cttccactgt
    11821 cagcggttca aaatacagtt tattggaggt gtcgtaatcc gtggaaaccg tttccggatt
    11881 gcagttgacc atgatggatt cgataccttc atcgcgcagg gcaaatgccg cgtgaacgca
    11941 gcagtagtcg aattcaatac cctggccgat tcgattgggc ccgccgccca aaaccattac
    12001 cttgcgattc ccggttacag gcacagtatc cggcgcgttg taggtggaaa aataatacgc
    12061 tgcattttcc acgccgctga ccggtacggc ttcccaggct tccgttacgc ccaacgcctg
    12121 ccgctgtttg cggatcgcct cttcctcttt gcccaaaatc tgtgccagat atttatcggc
    12181 aaagccgtct tttttcgcct gcgccagcag ctcatcgggc atctttttct ttttatactg
    12241 aagaattctt tcctccaggc cgaccaggtc cttcatctgc tcgatgaaat attttttaat
    12301 atgggttttg gcggacagcg tctcaaccga agcacctttg cgcagggcct catacatgat
    12361 gaattgccgt tcgctggagg gctcggagag catttccatc aattcatcca acgatttcgc
    12421 gttgaagtct ttaacaaatc ccaggccgta acgttttttc tccagcgacc ggatggcttt
    12481 ctgtaacgct tccttgtaat ttttgccgat gctcatgact tcgccgacag cgcgcatttg
    12541 cgtgcccagc ttgtcttccg aaccggggaa tttttcaaaa tcccagcggg cgaatttgac
    12601 gacgacatag tcgcccgagg gtgtgtattt ttccagcgac ccgtcccgcc agtagggaat
    12661 ttcatccagc gtcattccgg aagccagaat ggaggaaatc agcgcaatgg gaaaacccgt
    12721 ggctttggag gccagcgcgg aggaacgcga cgtacgcgga ttgatttcga tgacgaccac
    12781 gcggtctgtt ttgggatcgt gcgcaaactg cacattggtg ccgccgatca cctggatggc
    12841 ctcgacgatt ttgtaggaat aatcctgaag gcgtttctgc aattccggag taatggtcag
    12901 catcggcgcg gtgcagtagg aatcgccggt gtgcacgccc atcgcatcga cgttttcaat
    12961 gaagcagacg gtgatcatct ggtttttcgc gtcacgaacg acttccagtt ccagttcctc
    13021 ccaaccgatg accgattctt caatcagaat ctggccgatg aggctggccg aaatgccgcg
    13081 gctggcaatg aggcgcagct cttccagatt atagacaatg ccgcctccgg tgccgcccat
    13141 tgtgtaagcc ggccggacca cgacagggta acccattttt gtggcgattt tttccgcttc
    13201 ttcaacggag gtggccggtt cgcttttggg catatcgatg ccaagtttgt tcatcgtttc
    13261 cttaaaggcg attctgtctt cgccgcgctc gatcgcatca acctgcacgc cgataacttt
    13321 cacgttatgt ttttccagca cgcccttttt gtaaagttcc gacgtcagat taaggccggt
    13381 ttgtccgccc agattgggca ggatggcgtc cggtttttcg ttttcaataa tttccgttaa
    13441 tgcctggata ttcagaggtt ctatatacgt cacatccgcc atccccggat cggtcatgat
    13501 ggtcgccgga ttggaattca ccagcacgat ttcataaccg aggtttcgca gcgccttgca
    13561 cgcctgcgtt cccgaataat caaattcaca ggcctgtccg ataacaatcg ggcccgaacc
    13621 gatgatcaaa acttttttaa tatccgttct ttttcccatg gattttcctt ttcctcataa
    13681 ggttattttt tatattccgc gtcaacggtg attccgatgc cgccgcgcac ggcaaattcc
    13741 gcgctgactt tgcaccagcg gggtttcagc gctgctacca gatcatccag aatgatattg
    13801 gtgacatgtt cgtggaaaac accctcatta cggaaggacc ataaatacag tttcagcgat
    13861 ttggattcga caattttttt atccggtatg tattggattt taattttggc aaaatccggc
    13921 tggcccgttt tgggacagac gcaggtaaat tcttccgtct gcagggtgac aacgtaggca
    13981 cgtttttcgt tgtgattggg aaatgtctcc agtttgcgca ccggctttgc cgcctttccc
    14041 agcagtgtta atcctgcaaa atcatttttc tttttttctt tgggcatgtt tttgccttgg
    14101 gtaaaaatgg tatattcgtt taccgaaagt gggtaataat atcaagcata ttcgtcggga
    14161 aaaagaaaaa atgtctatct atctgttttt tgtgatagaa tcaccttaac ccagataagg
    14221 agaaaatacg gcaatgacag agaacaaaca gcctcttccc gaaaatacct ggaagtgtag
    14281 taattgcggc aatacggtgg tgcaaattac accaccggaa acgtgtccgg tttgcaaaca
    14341 gaaatgcgaa tttctgaacg taacctgcta tcagcccgat tgcggtttta cgggaatgga
    14401 caaaagactc aaataatctg ccggattttt gtccggcaga ttttgtttca tgactgaccg
    14461 gggatgacga tcttggaagc ttcctcacgt tccatttcct tggcttcggc gatcatcgcc
    14521 tgaaccgttt tttcaatggc caccggtaat ttttctgcag cttcggcaag cgtctttgct
    14581 ccttcgataa gacattgaac aggaatcgga ccgttgggcg acatcaactg ggtcataccg
    14641 gtaaaaatcg gttcgcggct ggcgtcaggc gagccgtcca gattcacagg attgagccgc
    14701 ctgatggtgg caaacgtcag gtcggtgaaa gcttcttcct tataaaggtt gttggtgtct
    14761 atcttaatat ccgtcatttt ggttgagccg ttattcattt ttttacctcc ttttcctaca
    14821 agctttctat gcacggaaaa aaacggaaac tgtacgctac ggcctcccct tttttcggat
    14881 catgactcaa atcggatgca agctgacaat cagttatgtt aaaataggaa aaacttcaat
    14941 gaatattttg acgcaatcaa gaacgaacga atccggtttc cttcaattcc cgcgctacgg
    15001 ttcatccgga aaaaaattaa accggcattg gcttaaaata aataaattta ataacttatc
    15061 cggttaatat ttcattgaca acccggggtg agtttcctat actgcttgaa agtgagagac
    15121 agatttttta tcatgatcac cttaaaacat tcctgagaga acaaaatgaa tcattacgac
    15181 cttttcaaat catggcatca acttatgaca cagacaaccg acatggcctg caataaaaaa
    15241 atcaaatcag aggcccccgt agagccgctc ggcgcactgg cttcatgggt ggatttgggc
    15301 accaggctgt cgcatttaac ctgggcaaga ttttccgaag atgcctggga gtattccatc
    15361 gacttctggc agcgttgcgt tcttttcatg gataccatgc gccggcgcgg caacaccatg
    15421 ctggagcatt atcagcaggg catgccgcct cttctggact atgaattcga agtcgttctg
    15481 gacgcacgca agtttccccg tccggtcaac tattcgctgc tgtacatcag acacccggaa
    15541 ggcaaggaac cgaaacccgg gaaaaggccc attctggtta ttgacccgcg agccgggcac
    15601 ggaccgggaa tcggcggatt caaaaaagat tccgaggtgg gcatgggtct tgcggaaggt
    15661 catccggttt acttcgcatc ctttacaccc gaaccgcttc cggatcagac catggaagat
    15721 gtcgaacagg cggagatccg ctttctggaa gaaataaaaa aacgtcatcc ggaccgtgat
    15781 tttcctatga tttacggcaa ctgtcaggcg ggatgggccg tcgccatgct gggagcggac
    15841 cgccccgacg tggccggacc gattgtcatc aacggcgcgc ccatgtccta ctgggcgggc
    15901 ggcgacggca ttaatccgat gcgtctgaca ggcggattga ccggcggcac ctgggcgacc
    15961 agtttcatgt gtgatctggg cggaggtctt tttgacgggg cctatctggt ggagaatttc
    16021 gagcggctca acccggccaa caccctctgg aaaaaagatt acaatctttt ctccaacatt
    16081 gacaccgagc agaaacgtta cctcgagttc gaacgctggt ggaccggatt ttacctgctg
    16141 aatgaggagg aaattctctg gattgtaaga aatctgttca tcggcaacaa gttggagaag
    16201 gggatgctgg atatcggcca cggtcaccgg attgacatga ggaacatgaa ggatccgctc
    16261 gtcatcttcg cgtccagcgg cgacaacatt acgccgccgc agcaggctct gcactggatt
    16321 tcggaagtgt acccgacaac cgaggatctt gtaaaagccg gacagcgcat cgtctatctg
    16381 ttgcacagtc acatcggaca tctcggcatc tttgtttccg cctccgtggc gaggcgcgag
    16441 caccgcgcga ttatcgaaca tatcgagaag atgcagaaat taaagcccgg cctgtatgaa
    16501 atgatcatcg aaggcgagac aggcgatcac gacccgcatc aagagcagtt ccgtgtccgt
    16561 tttgaaaaaa gggaaatcaa agacgttacc tgtccgattc cgaaatccgc atttgataaa
    16621 atggaccgcc tgtcggtggc caacgaaaat ctttatctcg ctttcggccg gcccttcgtg
    16681 aagtcgctga tccggcatcc gcagcaggca gcggctctgc gctggcttca tccggcgcgc
    16741 gtcagccgtt atgtgtggtc ggatcaggtg agtcccggga tgaagatttt cgactcctgg
    16801 gcgtcatggg tcaaggataa ccgaagccgg gcgcaggaat ccaacacctt cagttacatc
    16861 gagcggcggc attctgacaa catcgccaca tttctcgatg cctgccggga tattcgtgac
    16921 acgggccttg aagtaatttt cgaggcgatt taccgggagt aaaaattgcc tccgcaaaca
    16981 ccgagtgaat gatgaatcat ccgatcaaga gccgtcatcc gatcgccagc gacctggccc
    17041 ggcgcatgaa gcgcggcaag atagtttcgc ttgatgaggc ggtgggcgtc attcgcgacg
    17101 gagacacgct cgcgttcggc ggatttgtcg gcatcggctt tgccgaaggc gtcgccaggg
    17161 ccatcgaaaa acaatttatc gcgcagggaa aacccaaaga cctcaccatt atctattccg
    17221 ccggaatcgg cgacggcaaa gacaaagggc tgaatcacct tgctcatgaa ggactggtca
    17281 gccgggtcat cggcggtcac tggggactgg ttcccaaact gcagaagctc gccatcgaaa
    17341 acaagattga agcctacaat cttccccagg ggatcatctc acacatgttt cgcgatatcg
    17401 cggcgcataa tccgcgaacc ctctccaaag tgggactggg cacgtttgtc gatccccggc
    17461 acggcggcgg taaaatcaac cggaaaaccc ggaacgatat cgttgaactg ttgaactttg
    17521 acggcgaaga ctgtctggcc tataaaacac ggcctattca gatagccgta ttgcgcggaa
    17581 cgaccgccga tttggacggc aatgtcacca tggaacggga agcgttgaca gtggatgccc
    17641 tgtccatcgc catggcggtg agaaattcgg gcggcgtggt catcgttcag gtcgagcgta
    17701 ttgcggatcg cgggacgtta cgcgcccgtg aagtcaggat tcccgacatt cttgtggatt
    17761 gcgtcgtggt tgccgatccc gacgaccact ggatgacgtt cgcggaaaaa tacaatcccg
    17821 cctattccgg cgaaaccagg gtgccggtaa cgtctctgga gccgctgccc ctggatgaaa
    17881 gaaaaatcat cgcccgccgc gccgcgcttg agctgaaagt caattctgtc gtgaacctgg
    17941 gaatcggcgt gcccgaaagc attgctcgca tcgcagcgga agaaggtttg ctcgagtatc
    18001 tgacgctcac ggcggaaccg ggcgtcatcg gcggcattcc cgcgggcggc ctgagcttcg
    18061 gggcggcaac caatgtggaa gcgatcatcg accagcctta ccagttcgat ttctacgacg
    18121 gcggcggact ggacatcgcc tttttaggtc tggcgcaggc tgaccgggcc ggcaatctca
    18181 acgtgagccg cttcggttcc cgcctggccg gagcgggggg ctttatcaat atcagtcaga
    18241 acgcgaagaa agtggtgttt gcgggaacct tcgtcgccgg aaacttgcag gtagacgttg
    18301 ctgatgaaaa attgaaaata ataagcgacg gcgataggcc caaattcatc gatgccgtcg
    18361 atcagatcac gttcagcgga gccgtgggcg ctcaaagcgg acgcaccatt ctttacgtca
    18421 ccgagcgctg tgtgttccgt ttaagcgaga agggattgat gctcgtcgag atcgcgcccg
    18481 gcgtcaacct gcaaaaagac atcctggaga aaatgaaatt cacgccgctg atggctgaaa
    18541 aactcctgat gatggatgcc cggatttttc gtccggaggc catgggcctt aaagaagacc
    18601 tgctcacgct gccccttgcc gaacgtttca cttaccagcc ggaggaaaac cttttctttg
    18661 tcaattttga agggttgagc atcagatcga ccgaacagat cgatgagatc cgcgagcatg
    18721 tcgagcgcat ttgccgtccc ctgctgccga aaaaagtcca gacgattgtc aactacgaca
    18781 atttcgctat ccttcccgag ttgatcgaac cgtacaccgc catggtcgat cacgtcgtca
    18841 gccgttacta tgacaaggtc acccgctaca cgaccagcgc tttcatgcgg gtcaagctgg
    18901 gtgatctgct cgccgaacgt tcggtcgcgc cgcacgtctt cgaaaaaggc aaatagcccg
    18961 agagaaaaag cacttcagca attttctttg ccccggtaat caacggaaat attttccttt
    19021 aaaaaaacat aaatgatgat aaggagaagg ccagattcgt cagtaaagct ccctggaggt
    19081 gttcattgaa aggcggggat ttcaaatgat tgaaaaatta aggattattg tttcaagaat
    19141 attcgttgtt ttgttgtttg tgttgctgtg tttctccagc agccagtggg aagaggaggc
    19201 ctacttcgtg acggcagtcc tgttttttat tggcgtcatt ttggtggcaa ttgcttccct
    19261 gggaagatta tggtgttccg tctatattgc aggatataaa acggaccatt taatcacaca
    19321 gggtccttat tccatgtgca ggaatccgct ttatttcttc agtattctgg gtgctctcgg
    19381 catcgggttt gcttcagaaa cgcttctgat ccctctgctt attttcattg cctttgtcat
    19441 atattatccc tccgtcatca gaagtgaaga ggcaggaatg atgaaattac acaaaaatga
    19501 attcgccatg tatatgcaaa aagtcccccg ttttttcccc aaattatctc ttttgaatga
    19561 accggaagaa tatattgtaa gacccaaggt gctgaagaga cacatatttg atgctctctg
    19621 gttcatctgg atcgtcggga ttcttgaagt tatcgaagcg ctgcatgagt tgcacattat
    19681 tcccatacta ttcaaaatct attgatgttt ctgtttccgg gtcctgcccg caatgcttcc
    19741 tcgctttctg atttctgcat gcatcattcg caagaattgc atcttcacac gaatgcggat
    19801 tatcaggcag aaagaatatt catgatgaga tgaatttttt gcggccttac cgggggagca
    19861 aggctatcaa cttttttcca tcgtcttatg gaactgaact ttcggccatt cttccatggt
    19921 atggccgagg cgccactcgc ttaacgccag atagaccaga ttcccttcgg tatccagagc
    19981 gaggttcgtt tgatttttct tctgaaaatc ttccatgatt ttggcgtcgt cgcaggtcac
    20041 ccagcgggcg gcggcgtagt ctgtcttttc gtaatcggca taaacgctgt attcgtcttt
    20101 gaggcgcgac atcgtgacat caaactgcaa agcgcccacc gcgcccagaa tattatccga
    20161 accccataag ggcctgaaga cctgaatcgc tccttcttcg gaaagctgca tcagaccttt
    20221 ttgcagatgc ttcatcctga gcggatcttt gagccgcacg cggcagaaat gctccggcgc
    20281 aaaatgcgga atgccggtga acttgagcgg ttccttctgc gtaaaggtgt cgccgatttt
    20341 gatggtgccg tgattgtgaa ggccgatgat gtcgccggga taggcttctt cgatgttggt
    20401 tcggtcctgc gccatgaaaa tcgtcgcgtt ggccagagag acttccttgc cgatgcggtg
    20461 atggatgacc ttcatgccgc gctgaaattg tcccgagcag atccgcagga aggcgatgcg
    20521 atcacgatgc gccggatcca tgttggcctg gattttaaaa acaaatccgg aaaaatcctc
    20581 ttcctcgggt tccacggctc tggtggtcgt cggtcgggcg gcaggcggcg gcgcgatctc
    20641 cacgaacgca tcgagcagtt ctttgacgcc gaagttgttg atggcgctgc cgaagaaaac
    20701 cggcgtctgg ctggctttca aatattcctt caattcaaac ggattggccg cgccttccag
    20761 cagcgcgact tcctcgcgca gttcatccgc ctggctttcc agcaattcat cgagcagcgg
    20821 atcggacaaa tcgttgatgg tgatgccttc ttccgaacgg cgcgctttcc ccggcgtaaa
    20881 caaatgaagt ttcttttcaa acatattgta aacgcccttg aaacgcttgc ccatgccgac
    20941 aggccaggac agcggcgagc attcgatttg cagtttgtct tcgatatccg aaaggatttc
    21001 cagcggcggc agacagtcgc ggtcgagttt attgatgaag gtgataatcg gcgtattgcg
    21061 caggcggcag acttccatca gcttctgggt ttgcgtctcg acgcctttgg cgccgtcgat
    21121 caccatcagc gcgctgtcca cagcggtgag tacgcggtag gtgtcttcag aaaagtcctg
    21181 atggccgggg gtgtccagca gattgatgtc gcagtccgcg tattcgaact tcatgaccga
    21241 cgaggtcacg gaaatgccgc ggtctttttc aatggccatc cagtcgctca gggcatgttt
    21301 cgacgctttg cgcgctttga ccgcgccggc catctgaatg gctccgccga aaagcagcag
    21361 cttttccgtc agcgtggttt tgcccgcatc gggatgactg ataatgccga aggtgcggcg
    21421 gcgctcaatt tccttttgta actgtttatt caaaattgcc tctttgtttt atataatcag
    21481 ggggcgtata ttccttatta tgatccatct gtcaactaat atcctgaagg cctgacgtaa
    21541 accagaacat cgttgagcgt gtcttctttg atcgcgcctt cagcggtcaa ttcctttaaa
    21601 taggcaatcg tttcattcag cgccagaaat ttatcaaaat cggaaagcgt tggtgccagt
    21661 gtctcgtgag aaatgtcgcg agccgttcgg ggttgtgcat gcacgcgatc gagaaccatt
    21721 gtcttccttt tcgaatggtg ttcgcgtata tcggctatgc gttcttgcaa atccacaaaa
    21781 gaaattccgt ggccgggata aacggcagtt acgcacaggt ttttcagagc gttgagtgaa
    21841 tccagataag cggccaatgg ccggaacgcc tcatcgaaga tgtcaggact taaaaccggc
    21901 gcaatgtacg gcagcacatg atcaccggac agcaggaatt tcatctctct gcagaaaaag
    21961 cagacatgtc cggcggcatg tccgggcgta gaaattattt caattttcca gttcccgaat
    22021 tcgcggacgt ctttatcacg cagaaaatcc gcggcatcaa aatcggcaat gatgccgcga
    22081 atgttttcat attcttcaat aaccagaccg atttgttctt gggacatgcc ctggcgcgca
    22141 tagtagattc gcaactgttt aatggttgga ccggcctcgc gaaaatgccg gtactcttca
    22201 aaagccgcct gggcaagatg aatttgtgct cccgaccttt tttgcaggac tcccgccatg
    22261 ctgcagtgat ccgagtgcac gtgggtcaga tagatgtgtt gaatatcttc gatgccgaaa
    22321 ccgatgccgg ataaatcgtt gcccagagtt tcaaaagcct gcggtgtatt cagccccgtg
    22381 tcaaaaagcg ccagttttcc ttcataaata aaaagatagg cgttcacgtg tcttaagcga
    22441 tagggcatgc gcaacgtaat ctggtaaaaa tctttttcaa ttttactgat catatcggat
    22501 atcgcgatcg atggacatta ttcttcgcgg cggcttttca tctggcgttt ggttgcatcc
    22561 agttccattt ttcttaagcg aacgcttttg ggcgtcacct ccaccagttc atcttcggca
    22621 atgaattcga tcgcctgatc gagcgataaa atccgcggcg gccgtaaaat gaccgtggcg
    22681 tcggatgtgg atgcccgcat attcgtcagc ttcttttcct tgataatgtt cacgttgaga
    22741 tcggaactgc gattgcgctc gcccacgatc atgcccgcgt aaacatccgt gccgatttcc
    22801 acaatcattt caccccggtc ttccatcgcg taactggcgt aaggcgtaac gcggcccgag
    22861 cggtcggcaa ccagcacacc tgtcgttcgc tgcgggatgg aaccgaacca cggcgcgtaa
    22921 tcttccagga gtgaattcat caccccgccg cctttggtat ccgtcaaaaa ctgattgcga
    22981 aagccgatca gtccgcggga aggaatcaga aactccatgc tgacccggcc cgaccctttg
    23041 ttgaccagac tttccatgcg tccttttctg atggataatt tttcggtaat tttccccacg
    23101 aattcttccg ggatatccag gaagagtctt tccaccggct cgcacgtttt gccgtttacc
    23161 tgccgcgtga tgacctgcgg acgcgacacc atcagttcgt aaccttcacg gcgcatggtt
    23221 tcaatcaata ccgccatttg caactcgccg cgtccgcata cttcaaaggc gtcagtccgc
    23281 tccagttttt tgattttaat ggcgacattt ctcaattgct ctttttccag ccgctccagc
    23341 aggtggcgcg aagtaagata tttcccttct ttcccgccaa aaggactgtc gttgacataa
    23401 aacatcatgg acacggtcgg ttcatccacc agtagacgag gtagcggccg tggttcagcc
    23461 aaagatgata tggtgtcgcc gattttgaca ttttccacgc ctgataaagc aataatatca
    23521 ccagactcaa cggactgggc tggttttttt gccagaccaa aaaaagtata aagggcagtc
    23581 agcttgacgc cgggaattat tttatattca gtgcaaagac tgtaattggt attcatctct
    23641 aacttgccgc tctgtagacg gccgacggca atctgtccga cataaggatc ataatccaga
    23701 ttggtaacca gaaactgcgt aatagcttca tcgtctccct gcggcggagg tatgtattca
    23761 atgatggtct gtagcagcgg ctgcaaatcg gcactgtcat cgcctatttt ttcatggctg
    23821 ataccggcct tggcgttggt atagagaata ggaaactcga tctgatgctc ttcagcgccc
    23881 agatcgatga acagatcata ggtttcgctg atgacttcct cgatccgggc gtcgctgcgg
    23941 tcgattttat taatgaccag aataatgggc aagcgcaggc cgagcgcttt tttcaaaaca
    24001 aaacgggttt gcggcagggg accttcgctg gcatccacca gcaggattgc gccgtcaacc
    24061 atgttgaggc tgcgctccac ttcaccgccg aaatcggcgt gaccgggcgt atccacaatg
    24121 tttattttga catcgttgta attaacagcg gtattcttgg ccatgatggt gatgccgcgt
    24181 tcccgttcca ggtccatcga atccatcacg cggtcttcca caacacggtt ggcgtgaaaa
    24241 acccccgtct gcttgagcat ggcattgagc agcgtggttt tcccgtgatc gacatgagaa
    24301 ataatggcaa tattgcgtaa gtgaaacttt ttcatcctat taaaatcctt ccgggtcctg
    24361 attgtttatt gaaataaccg agtgacagcc gttttgagcg caaccggttt ttgctttaga
    24421 ctaaaaaccg taatttgagg gggttatgca ttaattttct tgacttgtaa atgtcttttt
    24481 cctatgctcg caaaacccgg cttcaatata ttctttttcc aggaataaaa acatgctgaa
    24541 tttcttaccg tccaaacttg taggagcgat tgcaggactg ctgcttgttg tcaatgtgtt
    24601 gttctggatc actattttat tcattttcac ccttcccaaa attctcatcc cctggccgtc
    24661 cctgctgaaa gtcatcaaca aaattctgca gtggatcggc gaaaactgga ttgcctgcaa
    24721 cagcggctgg atgtggctga cgcagaagac acggtgggat gttcagggag ccgagaacct
    24781 gaatcatgaa ggctggtaca tggtggtcag caaccatcaa tcgtgggtcg atattctggt
    24841 gatgcagcat ttgttgaatc gtaaaattcc tcttttgaaa ttctttatca agcgggaact
    24901 gatcaaggtg cctttgatgg gtttcgcctg gtgggcgctg gacttccctt tcctgtatcg
    24961 ccacagctcc gaatatctga aacagcatcc cgaacaggcg ggcaaggatt ttgaagcaac
    25021 ccgcaaagcc tgcgaaaaat tcgccactat tcccaccagt gtgatgaatt tcctggaagg
    25081 aacacgtttt accccggcaa agcatgacaa acagaaaagc gaatacaaat atctcttgcg
    25141 tcccaaatcc ggcggactgg cgctggcctt aaacgttctg ggcgaaaaat tcagttccat
    25201 gctggatatc accatcgttt atcccgacgg cattcccggg ttcggagatt tcctctgcgg
    25261 cagggtcaaa cgcatcaccg tgcgcatgag aaccatagaa atcccgaaga cccttttgca
    25321 gggcgattat gaaaatgatc aggcgtttcg tgattccatg cacaaatggg tgcagaaact
    25381 ctggcgtgaa aaggacaagc agatacaagc gctgatgaaa aaatgaagcc cccgtgagga
    25441 atgacgttgc cggcatgaaa ataaccggat ggcctgccgg agaagatttt ctttcgtgaa
    25501 ctgaagaaaa aagcggtgct gtttccgtaa ttgacgaatt ctgaaaatta tgcaatattt
    25561 caggcaatta aattattgcg acatcacaaa aaaaataact cgtctcagaa caaaacaaat
    25621 taacagagga ggagttaacg gctaatgagt aaaacattag ggaaaatttt tactattctg
    25681 gcggtcgtta ttctggccgg tgtttttaac gccaatgcgg cagaagaagc gcgcgacggc
    25741 cgtttcatcg cctacgatga cggcacagta tgggattcgc agagcaattt gttatgggcc
    25801 tcccacgata acggaagcaa tataaactgg tcggatgcgg aggaatattg caagacgtat
    25861 gacggcagcg gttacaaggg atggcgcctg ccgacaaaag atgaattgct gaccttatac
    25921 gaccggtcta tcttcggcaa taacgggtat cgtctgacaa aactgattac cctgagcggc
    25981 tcgtatccat ggacatccga cgttcagggt tcggaagccc tggtggttgg tttcgacaaa
    26041 gaccgtcaaa tgtggtttcc cgagtccgtc aaccacaaaa gcagaaacag ggtactgccc
    26101 gtgcgtttcg gcaaggaagg caagatgact gtagccgaac cgaagcagga agaagtgccg
    26161 ccgctgcctc cacctccgcc gccggaaacg atagcgccgc cgcctcctcc tcccccgccg
    26221 gtgcaggaga aggtctccat ttccctcaat attgaatttg atactgcaaa atcgttcatc
    26281 aagaaaaagt atcacaataa catcaagcaa gtcgcggatt tcatgcagac gtatccggat
    26341 accaacgttg ttatcgaagg ccatacggat aacgtcgacc gtttcaataa tcccgaaaac
    26401 aatataaaac tctcccaggc acgggccgac agtgtccgca aatatttgat cgataaattc
    26461 ggcatcgatg agtcccgcat caccgctgtg ggttacggac cctccaggcc gatcgccgac
    26521 aataatacac ctgagggacg tatgaagaat cgccgggttg aagcggttat agaaaaaatg
    26581 gttacaaaat agtttccaaa gctttcagaa attcaacaca aaatgccccg tgattgcgca
    26641 atcacggggc atttctcttc tcacacaaga aaatctcttg acaaaaaaag gattttttac
    26701 tttaatttaa ataaatagtt aaaataactg tttagtttaa aatataccgc aatcctttgt
    26761 attttataat attctaattt aacataaaaa tatggaccgc aagcgcaaag ataaatcaga
    26821 taaaacatca gaacgcatta tggcagcagc cagaacggtt tttgcccaaa agggatacaa
    26881 cggcgcacgc attgatgaaa ttgcagcgcg cgccaatgta aacaaagcaa ccatctatta
    26941 ccagatcggc gacaaggaca cgctttatac cggcgcgctc catcaggtga tcggtcatgt
    27001 cgcccgggac atcgccgacg ccgtttcgcg caaagttcat ccggaagaaa aaatgaaggc
    27061 ctatatcact ttcatcgccg gcgccgtgga ggcgaatccc gatctgccgc cgatgatgat
    27121 gcgcgaaatc gcttccggcg gcgcaacgct tccgcgactt gtcatcgagg atattgcctc
    27181 ggttatgagc atcctggcgg gcattctgga ggccggtaaa gaacagggga tattcatcga
    27241 aacatcgcct tttctgattc acatgatgat tatgggaacg attctttttt ttaaaaaagg
    27301 gtcgcccatc aaagacaaac aatcctggct gccggaagcg cttctggcgt atgatcaaaa
    27361 aataagcgcg aacctggggg aggaagtggc gtctttagtg ctcaaagcga taaaacaata
    27421 accatctcaa tcaaaacgga ggtcggcatt gaaaaaaaga atcatttttg ttgtctttat
    27481 cattttcttt ctgattgtcg gagcgctcgt ttatttcggc cagaaaagta accgggagaa
    27541 agagctcgta tattccggca cgatcgacgc aatccaggcc aatctgtcgt ttcaggtagc
    27601 cggccgcgtt tcgcaggttt tcgttcagga aggccagtcg gtctcgcaaa atcagatgat
    27661 cgccgaactg gaccgttcgg aattcgaatc ccgcttcgag caggcccggg cgaatctgga
    27721 acgcgcgcaa aaaaccagaa gacaactgga gacggtgctg gaactcagcc ggaaaacgct
    27781 ccccgcggaa gttgcccggg ctaaagccgg cgtccagagc gcaaaagatt ccttgaagga
    27841 tgcggaaaaa aattacaggc gctttgaaga gctgtttggc aagaacgtgg tttcggaaaa
    27901 agaacgcgac gccctgagac tgcaatatga cgttgcgcaa tcacgcctgg ctgaaagcga
    27961 atccgttctg aagcttgcgc aggggaatct gaccagaatc gatgcggtca aacaggatat
    28021 cgacgtggcg gccgcacaga tcaacgcggc tgcggcttcc ttaaatcagg cggaaatcca
    28081 gttgaactac acgcaattga aatcgccggc aaacggcgtc gtcaccagcc gcaatatcga
    28141 gccgggggaa acggtaacgc ccggcaggga agtcattacc gtttccgatc tgtcccgcgt
    28201 tgatttgaaa atcttcgttg atgaaacgga aatcggcaag gtcaagccgg ggcagaaagt
    28261 cgatgttcag gttgacacct tcccggggaa aacctacacg ggctatgtgt cgtttatttc
    28321 tccggaaggg gaatttaccc cgaaagttat tcagacgacc aaggaaaggg taaaactcgt
    28381 ttatctggtt aaggtatcca tcggcaatcc ccatcttgaa ctcaaagccg gcatgccggc
    28441 agatgcctgg ctcaaatagc ggagtgcgtc catgaccaag gcaccggaaa gccccccgtc
    28501 catggtggac gtcagaaatg tgaccatgcg ctttaaaagc gtcgaggccg taaaggatat
    28561 ttcattgcag attcccaaag gacacgtgct gggattggtc ggctccgacg gcgccggcaa
    28621 gtccacgctc ctgcgtcttg tcgcgaccat gatacaaccg accagcggca acatttacat
    28681 tgacggtctt catgtcgtta ccgataaaca aaaaataaaa aatatcatcg gctacatgcc
    28741 tcagcggttc ggactgtatc aggatctgac cgtcgaagag aacatggatt tcttcatgga
    28801 catctttgcc gtgccgcggc gcgagagaaa agagcgcaaa gaaaaatatc tgggcttttc
    28861 caatctgctg ccgtttacgg accggatggc gggaaacctt tccgggggga tgaaacagaa
    28921 actgggactg gcatgtgtgc tggtgcatga gccgcagttg ctgatcctgg atgaaccgac
    28981 caacggggtg gatccggtat cgcgcagcga attctgggag attctggaaa acatgcagaa
    29041 agaaggcatg acgatcatgg tgtccaccgc ctacctcgac gaaggagaaa agtgccacag
    29101 gctcggactg atgcacaacg cgatgcttct ggatctggcg ccgcccgccg tcattcgcgg
    29161 cgggtatccc acgctggaag aggcgattat tgaacgaatc acacaagcgg acggagcgct
    29221 ggcacatgac acctttcaac tctgattcca tttccgtccg tcatctggaa aagagattcg
    29281 gcaattttgt tgccgtcaac aaaatttctt tttccgtcaa aaaaggcgag atcttcggat
    29341 tcctcggctc gaacggctcg ggcaagtcca caaccatccg gatgctctgc ggcatcatta
    29401 caccgacatc cggcagcggc acagtcgccg gacatgacat cgtcaccgag gcggaagacg
    29461 tcaaacaatc catcggctat atgtctcaga aattttctct ttatgaagat ttgacgcctt
    29521 tcgaaaatct gcgtttttat ctgggcgtgt acaatgttcc tcccccgcaa tggcgcgagc
    29581 gcattgactg ggtgctgaac atgacccgtc tgcaggacgc gcgcgaccgg aagacccggg
    29641 agcttccgcc gggctggcgt caacgtctgg cgctgggctg ttcgcttttg cacaaaccgg
    29701 acattctgtt tctggacgaa ccgacgtcgg gcgtggatcc ggtcacccgc cggcacttct
    29761 ggagtttcat caggaaactg gccgaaggcg gcatgaccgt ttttgtcacc acgcattaca
    29821 tggatgaagc gcgtttctgc gaacgaatcg tgatgatcaa tgaagggctg attgtggcgg
    29881 cgggaacccc cgccgacatt attgccggag cctgccccgg cgcggcggac gccgatctga
    29941 cagacgcgtt tatcaaaatc atgaagagag acaaagcatg aagttcatcc gactcaaggc
    30001 catcatccgc aaggaatttt atcacctgat ccgtgattac cgcagccttt atctggcttt
    30061 tgccattccg cttatcctca ttattttgtt cggttacgcg ctgagcctgg atgtggataa
    30121 tgtggaaacc gtcttcattg attacgacca cagcgacctc agccgggatt tcatccgcaa
    30181 actcgacgcc tcttcctatt ttcatgtttc gggcatgctg ccgtcaacaa aggatgtggt
    30241 tcgttatctg gatgaggacc gcgcccggct ggccattgtc atcccgccgg atttcaaaaa
    30301 aaacatcggc gccgaccggg aaacgccgct gcagattatc atcgacggca gcgatccgac
    30361 cttcggcaac atcatgcgcg gctacatcag cgcctttgtc gaacaataca accggaagct
    30421 cctgatggac tttctcaacc ggcagggaca ggagcacatc aaccctcccg tggatggacg
    30481 aatccgcatc tggttcaacg aagacatgga aagccgcaat ttcattattc cgggaatcgt
    30541 cgcggtgatc atcatgatcg tcggcgcgct gttgacgtcc ctggtcattg cgcgtgaata
    30601 tgaaaacggc accatggaga ccctgaaatc tctgccggtc cgtgccggag aattgctgct
    30661 gggcaaagcg ctgccctatt tcttcatcgg gttgaccgac gtgctgattg cgattctgtt
    30721 gagccaactg ctgttcggca tcgtgatgaa gggcagcttc tggctgatga ttctatcgtc
    30781 ttcactgtat ttaggcgtcg cgctgtcgct ggggcttttg atttccaatg tgacccaatc
    30841 gcagttgctg gccaatcaga ttgcgatcat ggcaacgtac ttgccgtcgt ttctgctttc
    30901 cgattttgtt tttcccatcg tcaatatgcc ggccgtcatc caggccatca ccttcatcac
    30961 gccggcgcga tatttcattg acatcctatc cgggatttat ctgcgcaatc tcggatttgc
    31021 ctacttgtgg ccgagcatgc tggtgttgct gctcatgttt attgtcctga catcaatgaa
    31081 ttacagactg ctgagaaagg aagggctatg aactggctga gaatcagaga gctggtccgc
    31141 aaggaattca ttcagctttt cagcgataaa cgcaaccgcg cgctcatggt ggtttttccc
    31201 ttcatcatga tgctggtgtt cggttacgtc gtcaattacg acatccggaa cgtccggatg
    31261 gcggtgctgg attattccaa aacccatgag agccgttcgc tgattaaaag ttttgaaggc
    31321 ggcaacatct tccacgtgac gcacaacgtc atccatgacc ggcagatgac ggaacttctc
    31381 ctccggcaaa aaatcgatat gggaatcaag atcagccatg attttgcatc ggtgatccgc
    31441 gcgggacaaa cggcgccggt tcagttgctt gtggacggtt cgatgagcaa tatgacgtcc
    31501 atccgggtgg cgtataccgc gacgattctg gagaaattca accgcgaaga attgcgcagg
    31561 ctttatccca tggatatgcg ttacgggcgg gtcgatgcgc gcatccggac ctggtacaat
    31621 cccaatctcg acagtcagtt cttttttgtt cccggcatcg tcgcatttct gatcatgctg
    31681 acaagtttca tctttacgtc catagccatc atccgggaaa aggaggacgg caccatggag
    31741 cagctgattg tcaccccgat cagatcctac gagtttatca tcggcaaaac cattccctat
    31801 atcctcattg cgcttttgca actgtttgcc gtaaccggcg ttgccgtcta ctggttcgag
    31861 attcccttta aaggcagttt tctgctgctc ttgctggccg cctgtttgtt tttgttgagc
    31921 acactgggca tcggactctt catttcgacc atctcctcga ccaaacagca ggccatgatg
    31981 acggccttct ttttcgtggt gccgtttttt atgctcagcg ggttcgtgtt tcccattgcc
    32041 aatatgcccg accccgtgca atggatgacg tacctcaatc cgttgagatt ttttctggtc
    32101 atcatcagga atatttttct gaaaggcgtt ggattcgatg tgctctggcc gcaatacctg
    32161 gcgttgacca ttctgggagc aattgttttt accggcgcca ttgcccgttc taaaaaacgt
    32221 ctggattagt gaagcgttct catatattat acgctctcaa cgtcacacct cttatttttt
    32281 ccgccccggg tggaagggaa ttcaagggag ggggaattca tgctcaaggt tgtcgcggat
    32341 caaaaagcca tcaaaaaata tgccggactt ttcaataaaa agttcaaacc gtttgtcgcc
    32401 gaaacgatca aggtcaagct tggtcatcag ggagcgagtt ttccggcccg ggtgctctgg
    32461 ttgcaggaac tgggcatctg gaaattttcc caggctgtca aagaggttcg ctactggaac
    32521 gccttcggcc tggataagcc cggagcggcc agcgtgctgt ccattgcctc tgaaatcaat
    32581 ttcccttgga atcagatcga ccggaagatg ggcggcgcct ttgccgagga tcactgggga
    32641 aacgtctttg tcattcaccg cgggaagatc ggcggcggga aaaaaggcat cggcaaatcg
    32701 ctttttgaac aaaattaccg cggcgtctgg tcatttatgg aggatggaga caccatcagt
    32761 caggtagcgg tgatcggcaa tctcaaatca gagcgtttcg cttcgcagac ggcgctgttc
    32821 gtcaaaaaaa tagaaatgat gaaattgtcc gccgccgagt cggcgcaaac ggaaatggat
    32881 tttgcggaaa tcagttttcg ggaagacctg atcggaagcg aatcgccgct gccggaggat
    32941 gaaatcatct ttgcctgcga ccacgatctg gttgtcagcc gcctggccgc cgagctccag
    33001 cggctgaaat tcaaaacggg caacgatagc gaaagcgagc tctttgccgt tgatacatcg
    33061 ggaaacagaa tatcccatat ttttgacgtg gtgaccgatg ccaaagaaaa aaacgtgatg
    33121 gccgccgccg gtcgattaat gttgcagaca tcggcaggcg cgttgaaccc tcttcccgtt
    33181 ttggtgttgc cggacgatat catgaatcat tacaaatcgg atttgagacg aatccacatt
    33241 tgcgttatcg gttttcactg gcaggacaca caggcggttt tttccggact ggagaaaatc
    33301 agcatccgct aagatcgtcg tccggcatgt tgatttttta aaatgaattg gattgaggtc
    33361 taacgcggaa aagataatgc ccctgcgtca gatgttttac gccgcatccg gcttggggga
    33421 ctcctttgcc tcctgcgaat ccgcatgttc acgggcgatg tttctgaact gttcatgaat
    33481 ttccagaaac gcgtccacca tgtcgggatc aaagtgcttc ccttttgcgt tgacgataat
    33541 ctccacagcc tcggcatgcg gataggcttc tttatagacc cttttgctga tcagggcatc
    33601 atagacatcc gccagcgcca tcagtcttcc gctgatggga atggcatccc ctttgaggcc
    33661 ctgaggatag ccggagccgt cccatttttc atggtgcgta taggcgatct cttcggcaat
    33721 gtttaagaat gaaattttac cgaattgccg gacggaggat cggatcaggt tgcggccatt
    33781 ggtggtgtgt gtcttgataa tttcaaactc ttcgcccgtc aatttgcccg gcttgagcaa
    33841 aattttatcg ggaatcccga ccttgccgat atcgtgcagg ggagctgatt tatcgagcat
    33901 ctcaatgttc tcgtcattga gaaaagttct gtacttatcc cggtgcttca tgtgatcggc
    33961 cagcagcctg acataaagtc gcgtgcgctt gatatggccg cccgtttcct gatggcggtg
    34021 ttccgtcaaa ttggccaggc tctcgatggt gatatcctgt gtcagaagca gttcctgcat
    34081 atgggccaga ttttttttct cctccatccg gtattttaat gcggtcagaa acatgtagtt
    34141 gcagataacc gacgcaatgg gaaacgcggt ggccacaaaa aaacccatcc ggaagaaaac
    34201 ctgatgggtc gtcatccata atccgccggc aaaaagcagc atggcaacaa aaccggtggc
    34261 ggctttctgg tagctgatca acaggctcat ggccaacccc gacaccataa ccagcaggag
    34321 gataaagcca ttggaccaac cgggcaggga gatgaaatcc cctttcagaa gattgtcgac
    34381 agccgtggca tgcacctcga cccccgggaa tacgggaccc agcggggtgt tcaataattc
    34441 tttcaggccg accgccgatg tgccgacaaa cacgatccgg ccccgcaacc gttccggcga
    34501 cacgttgtta tccagaatat ccgctgccga tacgtactcg tatttttgcg cggaactccg
    34561 gaacttgatc agcatctggc cgtgccggtc aacgggcacg gcgttgcccc ggtaatggat
    34621 ggattgcaga tgattgcggt cttttttcaa aatgacatgg ttttcgtttt taagtttcag
    34681 cacggtggcg agggccagat tgggataaag ctgtccgtga tactgaatca gcatgggcag
    34741 acgccgcagc acgccgtcac tatcaggact gaagttgaaa aagcccgacg agctgacctt
    34801 gtccgacagc atgggcaaat tgcacagcac gcccgtactc accggaattt ccgtatgggc
    34861 gttttgattc acgtcatcat ttatgtatga cgatttgacg ggatgcagaa cacagcgatc
    34921 cgaccctttc ttcagctcgt cgaagtgaaa gatcgtgccg aggacaaagg gccctccggc
    34981 cagagtctga gcaagaatct gatcgttgtc cttcagactc cggggcagtc cgtgaagatc
    35041 aattttaatc ctgtagacgt cgcccagatc tttcagcagc ggtcccgctg acgttctgtc
    35101 cggctcggca aaaacaatgt cgaggctgat gatgtccggt tgcatggcga tcaccttgtc
    35161 cagcagtccg gccacgcggt accggggcca gggccattgc ccgtagcggc tgagactttt
    35221 ttcatcgagg tccacgatga ccagtttgct gctggcgtta ttgttcggaa attttgccag
    35281 aagaaaatcg tactccagat aatcaagccg ctgcatcaaa ttcggtttga aggcaaagat
    35341 gaccgcaaag cacagggtgc agaggatccc ccagatcaga atacgccgtc tttccggttt
    35401 gctggttgtg atcgcggggt ccgtttgatt tttgtccgat gcttgcttca tgaatgatca
    35461 gtgcgaatgc tgtttatcgg atggtgacct cgactctccg gtttttaggt tccgcaactt
    35521 catcagcggt tttaatcagg ggatttcctt ccccgtgcga ggtcacggtg atgtgggcag
    35581 gcacggcgcc ctgggcctcg aggattttaa aaatagcctg agccctttcc agcgacaagg
    35641 tgtagttgta gtttttttcg ccgaccgtat cggtatggcc tgaaatcaca atatctgtcg
    35701 attgcctgct ttgtatggtc tgaaaaatgt ccggcagaac cgcccgggac tcatcggtca
    35761 gctggtccga atccggcata aaatgcagga tgaatttcac cggcggcaga ggttcggcag
    35821 ccagagcgtc ggcaaaagtg tcctggattt cctcggcgct catggtcttt accttacccg
    35881 gcgctgtttt caaatccgcg accctgaccg attgattttt ttcgttcaaa atctgctgtc
    35941 cgccctcgtt ggccaccacc aattgtccga catggccgtc aggatccgga attaatacaa
    36001 ccgtgctccg gaccgcgcag cccgccaaaa caaggacaag ccccaaacag aatactgttc
    36061 cgattaattg cttcatgtcc gcctccatca tcagaaatgt ccggcaaatc gccgtccggc
    36121 gaaaagcgtg tgattgaaaa tcgtatcacc atgcgttacg ggcaaccatc caccttaacc
    36181 aggaaatgtg tgccgcggat accgatggtc gcttcaggcg tccggaactt gaccgattcc
    36241 ggagactggt gaccgataat gcctgaaaga taagacgcgg tccctctcac cattcgcgca
    36301 atcatggaaa acaccccttt ttcgggggca aacacatatt ggtcaatcac gatttcgctg
    36361 tccggcccca aagacaagat agtattgtct tcaaaaatga ttcccacaga gcttgacgcg
    36421 cccgtgcgta tggtgtcatt ttggaaaagc ctgtcgccga cggcaacggg catttgcctg
    36481 ccgtcgcgga cgacaaaaac agagcctttg cttgttttgg tttttcctac aggcgattcc
    36541 tccggcatgg cgatgcatgg cagcagaaac atcatggcaa gacaaaggat caagattctt
    36601 ttcataggta gactccctta tggttgacgt tgcggggcac ccgtatcaca ttcggaggat
    36661 cgccctccgg atgtctcttt ttccttcgat ggaaggtgtg tttttttaca aattatgtat
    36721 atcataacag ccccccttaa aaaagaacaa tctttaatcc ctatagaccg ataactgttt
    36781 gcattaataa agttatcggt tttcatcgga cgtaaggaga agctgttttc ccggtcccgt
    36841 aaaaatcaaa tatgccggca atttttttgc aaccattcat taaacacctg aaacaacatt
    36901 tctattgaca aaatcatggg gtataattat gatgcagtta aaattgatat caagagattt
    36961 ttgatgaaga aagatattcg tgccatcatt attatcaata ttataattag cttctacata
    37021 ggaggccggg gcgcggtata gtatcagctc gaaatcaaca aaattcaaat ccgctgccag
    37081 gcaaacccgg cagcggattt tttttaggag aggggacgat gaaaaataaa atactggttt
    37141 acgatacaac cttgcgggac ggcacgcagg gcgagcagac aaatttttcc gcggaggaaa
    37201 aactgcgcat tgcccagcgc ctggatgatg ttcatttcca ttatattgaa ggcggctggc
    37261 cggggtccaa tcccaaagac gcgcgttttt tcgaaatggc aaaaaacgcc gcatttaaga
    37321 attcgcggct gaccgccttc ggcagcacgc gccgcgcgca taccaaaacc gaatcgtgcc
    37381 cgaatttaaa ggctttgatc aaggccggaa cgcccgcatg cgccattgtc gggaaatcgt
    37441 gggacttgca cgtcaccgat attctcaaga cagacctcaa cgaaaatctg gcaatgattg
    37501 ccgattccat cgcctatctg aaatccatgg acaaagaagt tctctttgat gccgaacatt
    37561 ttttcgatgg ctataagaac aattcccggt atgccacccg ggtggtgaaa acggcgctgg
    37621 ccgccggagc cgattttatc gtcctttgcg ataccaacgg cggatgtctc cctcatgaaa
    37681 tttgtgccct ggtgaagaga atttccgcgc atgttccttt tgagaaaatg gccattcatg
    37741 tacacaatga cggcggtctt gccgttgcga atacccttgc cgccgttcag aaaggtgtcg
    37801 cgatggttca gggaacggtc aacggttacg gtgaacgatg cggtaacgct gatttgattg
    37861 cggtgattgc caatctgcag ttaaagatgg ggaaaaaatg tctgccgccg gaatccatca
    37921 aacaactgac gaatctgtct cattatatca gcgatgtggc caatgtgccg ccgctgaatt
    37981 cccggccgtt tgtcgggcgc agcgcgttcg cgcacaaagg cggcgttcat gtcagcgccg
    38041 tggtgaaaaa ttctgccgcc tacgaacaca ttgagccgga actcgtcggc aatcaccggc
    38101 gcgtgctgat gtccgacctg gcgggaaaaa gcaacgtgga atataaagcg aaagaactgg
    38161 gcat
//
LOCUS       DAOOLB010000017        35376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_966_length_35376_cov_44.342601, whole genome shotgun sequence.
ACCESSION   DAOOLB010000017 DAOOLB010000000
VERSION     DAOOLB010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 35376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35376
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_966_length_35376_cov_44.342601"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /gene="amrS"
                     /locus_tag="PLF77_03855"
     CDS             <1..657
                     /gene="amrS"
                     /locus_tag="PLF77_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPC08023.1"
                     /translation="TSRSPVIAFTYNEPTVFTEYLTDIARNARQQGIRCVMISCGFMN
                     EAPLAEMCETLAAIKIDLKGYSEDFYRSACQAELKPVLRSIRQIAKSRAHLEIVNLVV
                     PTLNDSDKMLKGLIDWVMGEVGPDVPLHFSRFHPDYQLRNLPSTPVATLERAYRMARD
                     RGLHYPYVGNVPDHPGNNTYCPKCGKIVVKRQGFFITGMNLRNGLCTFCKNRIAGVWT
                     "
     gene            674..2212
                     /gene="amrB"
                     /locus_tag="PLF77_03860"
     CDS             674..2212
                     /gene="amrB"
                     /locus_tag="PLF77_03860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPC08024.1"
                     /translation="MKWLRLLCFLPVIVFFLTSACQANKDAADGVREPAFAGKFYPAS
                     ADKLRLTIEKYLQDALPPRVKKPVAVIVPHAGYIFSGQICADGFRQAGHHPYDVVVIL
                     GTNHTSPGLQGVSLYPGEGFRTPLGIAAVDKTVIESLLTESPVDCMLDKSAHVSEHSV
                     EVIVPFVQIVFPNAKIVPAVVGTQDRHTSIRFGQALAKVLKNKNALIVASSDLSHYPS
                     RDNAVKVDRQTLDAVATLDPAVFQKTIQDTMSKNIPNLSTCACGEAPIIAALAAARLL
                     GADRGNVISYANSGDVSVGDSAQVVGYGAVVLTAGQKGISSSQTDLQKTAAVPFDLGT
                     ADKKALLAFARETLSRFFTTDTVPLARHFPAHLNHPRGVFVTLKKNGALRGCIGHLIT
                     DEPLVKLVGAMALQAAFNDGRFPPLTAEELPDVDIEISVLTPMKQAARPSDIMVGRDG
                     VVLSKDGRSAVFLPQVALEQSWSRDDMLDHLCSKAGLEKRCWRQDATFSIFQAIVFTE
                     SQLK"
     gene            2217..>4038
                     /locus_tag="PLF77_03865"
     CDS             2217..>4038
                     /locus_tag="PLF77_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08025.1"
                     /translation="MTVYLLSIGLISILGQVILLRELNVSFYGVELIYLFALGIWLFW
                     TAVGVIAGRRMALPSRHSPAVLFILFGMILFLDVAFIRSSRLIFGGVPGAYLDFFQQL
                     LVATLSILPVGLLSGLLFQQTAGLYVDSGRTLAAAYAVESAGGLIGGLFSTLLIILDT
                     QNLFAALICSIVSFATPLLGAGKSLGAFLRRTAIVLLAMFAGLIFNASPLHDISTRWN
                     HPNLLDSSDSPYGRITVTRMFDQISVFENDALSFETEGTDAEYFCHLVMLQHPHPRKV
                     LILGGGMEGIIGEALKYGPDHIDYVEINPVMTKLVMEHLPESIRQSLQSPEVRIIYAD
                     PRRYLTNSGRYDLILVGMPEPSSGQANRFYTQEFFKQCAAKLKSGGLLGFKLQTAENL
                     WTGPLIGRNASIYHALRSVFPAVLFLPGTTNIVTASFAPLPDTPDIMTGRLQHLQLET
                     RLISDHYIRYLFTNDRFAAVKNLLNEKKDLLNSDIRPVCYQYALIIWLSKFFPRATLF
                     DFSSIMEKTLLRYLPVCFLMVLPVLFLVSRFLPGLRRKILVAAAGFAGIALETILLLY
                     FQLKYGVIYRDIGLLLMSFMAGLALGALAVDKLMARFADHR"
     assembly_gap    4039..4138
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4139..4531
                     /locus_tag="PLF77_03870"
     CDS             <4139..4531
                     /locus_tag="PLF77_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08026.1"
                     /translation="ALGALAVDKLMARFADHRLGTRRCGIILVAGSCCLCAITALLIR
                     NSLPAGLWLTGLLLTATGFIVSGIFAYASLYEIDDQRAIISPLYAADLIGGCLGSVLV
                     SLIFIPLAGMDTTMWGMLLLSVYAFLLL"
     gene            complement(4550..6418)
                     /locus_tag="PLF77_03875"
     CDS             complement(4550..6418)
                     /locus_tag="PLF77_03875"
                     /inference="COORDINATES: protein motif:HMM:NF014589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase"
                     /protein_id="HPC08027.1"
                     /translation="MSPAILGISAYYHDSAAALLIDGRIVAAAHEERFTREKHTSVFP
                     VHAATYCLREGGRHLADLDGVAFYEKPYLKLERLLETYHAFAPRGLKSFMAAMPVWMK
                     EKIFMRKMLHDGIAGIGPGRVKLLFPEHHLSHAASAFYPSPFDKAAILTIDGVGEWAT
                     ATIGLGEGRSIKILRELDFPHSVGLLYSAFTGYCGFKVNSGEYKLMGLAPYGHADSGQ
                     TALYRRKILDHLVDIREDGSILLNMDYFDFATGLTMCRDEKWEKLFGIPRRQRESEIV
                     QSYMNMALAIQQITEDVVSRMARTARKLTGSNYLVMAGGVALNCVANGALLRQKIFDD
                     LWIQPSAGDAGGALGAAFAAWHVGMEQPRKSVSEQDAMAGAFLGPEFGSDDIKRTAKT
                     YQASFVHYEDFDELCAVVAERILRGLVVGWFQGRMEYGPRALGNRSILANPCLPDTQK
                     KLNLKIKYREEFRPFAPAVLEEDVAEYFDLDRPSPYMMLVAGLKEHRRRPQPENERQM
                     TLYERLYHMRSDLPAVTHIDYSARIQSVSKRTNERFWKLIKAYKQKSGWGLLVNTSFN
                     VRGEPMVCTPEDAYRCFMQTDMDCLVMGNDLFSKDDQPPWSEKNPWLASGTPYAKD"
     gene            complement(6428..6577)
                     /locus_tag="PLF77_03880"
     CDS             complement(6428..6577)
                     /locus_tag="PLF77_03880"
                     /inference="COORDINATES: protein motif:HMM:NF039953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5989 family protein"
                     /protein_id="HPC08028.1"
                     /translation="MDFFRDVWGFLKVRKKFWLLPIILMLLLCGALMIFAGGSAVGPF
                     IYTLF"
     gene            6601..7269
                     /locus_tag="PLF77_03885"
     CDS             6601..7269
                     /locus_tag="PLF77_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08029.1"
                     /translation="MFNVIRPEFVLAYFENRMLIFFPFFQLQRIVAEASPDKTDGQHK
                     NQKNQEQDQPGHHSAEDVRNAQPDHRPRLEYPGPNDIQKKQQQSKTVKKRYVRKKTCY
                     QTKQRGSHAGIFGLIGCDCVGNKFHFVFVIFSETYFDFNITSRSGKPLSSSWQRLFPH
                     RRLPQAVMIRNGHPKECCWSVLKIIHCGIPATGSPDDVRPGNGRFDFAKTTANACLDF
                     LDGS"
     gene            complement(7246..7428)
                     /locus_tag="PLF77_03890"
     CDS             complement(7246..7428)
                     /locus_tag="PLF77_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08030.1"
                     /translation="MSFFIVLFEDGSLKIYKSMQDREFLPEGARQFKCSSNVTLQDLC
                     LWLDGGCKKLRTIKEI"
     gene            complement(7464..7889)
                     /locus_tag="PLF77_03895"
     CDS             complement(7464..7889)
                     /locus_tag="PLF77_03895"
                     /inference="COORDINATES: protein motif:HMM:NF023696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HPC08031.1"
                     /translation="MKKILFSFVMIVCLFWTTVVFAELKEGLWEITSQVEMKGMPQSM
                     PPTTFRQCITKSNPVPQNKDKNFECKIISQKVSGGTVSYQVECTGKEGVVQTSGTTTY
                     TDKAMNGASTTSFKMKGQPAMTMSNKIRGKYIGPCPKAQ"
     gene            complement(8039..9616)
                     /locus_tag="PLF77_03900"
     CDS             complement(8039..9616)
                     /locus_tag="PLF77_03900"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPC08032.1"
                     /translation="MSGEKNTTQDLVLSVDAGTQSIRAALIDVRGNIKAIVKTPIQPY
                     FSEHPGWAEQRPDYYWDVFCKTTHQLLQDQEVLKKHVKGVTLTTQRATMVNVDQNGNP
                     LRPAIIWLDQRKADSRKILPAPLRPLLKTVNLLDTLENVIQECEANWICQHQPDIWEK
                     THKYLVLSGFFTHRLTGEFVDSTGNNVGYLPFDNKTYQWAGKFDFKWRIFKVEKEKLP
                     RLVKPSEMMGAITKKASAETGIPEGLPVFAAGSDKGCEILGSGCLTPETGCLSFGTIA
                     TFDVATPKYVELHPMIPPFPASVPDTYYTEVSIFRGFWMVSWFKEEFGLQECMLAEKT
                     AEPPEKFFDSLIQDVPAGSKGLMLQPYWTPGREVDAFAKGSIIGFGDVHSRAYLYRAL
                     LEGLVYGLREGGELTQRKTKIPVTRLKVSGGGSQSDAAMQITADIFGIAAERPHTFET
                     SALGAAIDAAVGLKLYSDFASAVTNMTRTGRVFEPDKDNHGLYDNLYRRVYLKMYERL
                     KPLFRDIRDITGYPAGN"
     gene            complement(9667..11352)
                     /locus_tag="PLF77_03905"
     CDS             complement(9667..11352)
                     /locus_tag="PLF77_03905"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HPC08033.1"
                     /translation="MISHYFRPNWTEKAPAAGTYRSIFKYGDIHSFKHPSDAWYRMIK
                     EEFHLNDKDFASRVKEGLEPVSLNRAQALKPQQMDCLRNIVGGENVALDDYSRVKYAS
                     GKTTEEMLELRLGIIREVADVVVHPRDKQDVQKIVQYCHQENIPITVYSAGSSVNFGC
                     RPVRGGVSLVVSTHMNKLLEINEFNQTARVQPGMFGPAYEEGLNKAPELFKTKHRYTC
                     GHFPQSFEYSTVGGWVVTLGSGQASTYYGDAYDIVFSQEYVTPAGTFKTLDYPATATG
                     PKVNDIMKGSEGSFGILVEVTMKIFRYMPENRRRFSFMYPTWEAAVNASREIMQSEFG
                     KPAIYRISDPEETDRGLKLYGMPDFVDKILVMKGFKPMQRCLSLGNAEGDCDYTKLVV
                     RKIKTVAKKHGAISLTGYAAKKWEHTRYTEPYMREDLGDYDILIDTLEAAVTWDNLHR
                     LHQGVRAYIKNRPQTMCMTHASHFYPQGTNLYFIFIAHMKDPEEFRKFQRGIIDNIQK
                     HGGSLSHHHGVGRMIGPWMEKHLGKEQLDVLRTLKKHFDPRNIMNPGGQLGLD"
     gene            complement(11402..13048)
                     /locus_tag="PLF77_03910"
     CDS             complement(11402..13048)
                     /locus_tag="PLF77_03910"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HPC08034.1"
                     /translation="MKRFIENYTGEKFDVAVIGGGISGASVAYEAATRGLKVALLEKN
                     DFSWATSAVTSKMIHGGLRYLVNGEVGLVRESLRERRVLENIAPNFVYPWPMMMTHYK
                     KPLKNNKWVVKVGMLVYDVLSYDKNITWDPCKKIPVHRTISARQVLQDEPHVRAEGLT
                     GASIFYDCTSLFPERLTLAFIKSAVAHGARAANYARVEGFLMDGAKHVLGVKVKDLFS
                     GKMHNVPATVTINCGGPWADIVLGMAKPDGQVNATLRRSEGIHIITSKKLLTGNYTIG
                     SMTPEGRHFFLIPWRNHALIGTTDKPFNGNPDDYRVTKESIMELIADVNSSFGDGKLS
                     YADVKHTYGGLRPLIEKETSETYSSSRKYEIYDNKDDGLDGLITVEGGKYTTSRKLAE
                     NCLAIVAKKMGRNLGRSVTDKQYLTGCDIKDLCLFIEEAKRPADGLSGATLEYLARNY
                     GTVYRDVVGLAREKKSLSETLNEDGEIMAQVVYAVRDEMAQTLADIVMRRTGIGTLGN
                     PGDDVLRKVAQVAADELHWDQQRMEKEVEAVVNLLKIPVD"
     gene            complement(13054..13770)
                     /locus_tag="PLF77_03915"
     CDS             complement(13054..13770)
                     /locus_tag="PLF77_03915"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HPC08035.1"
                     /translation="MTRKVFARPVTRSRLHEEIVTVIQQQIMSGQIAPGEKLPTEREM
                     AENFNVNRATVREALRKLENIDLLEIRHGDGLYVKDYLNSGNFDLIKAAVKMDGGTEI
                     IFNILEARRYLVPQMAYLAAQRRTSSDVDDLKQLLSEKNITMLEKDIRIHQQIARCTH
                     NLLCTIGLNFFNQIFRDYGHLYFNDPENVERSRQFHDEIFEAIRRKKADDARNIMRDV
                     LIYAEEAVKNKIKRPTFDKE"
     gene            complement(13974..14804)
                     /locus_tag="PLF77_03920"
     CDS             complement(13974..14804)
                     /locus_tag="PLF77_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPC08036.1"
                     /translation="MPFLKRGNATIYFEDDGWGDPIIAVHGLIENTTYWKTTAEEIIK
                     KYRFIPMDMRGHGKTVVEGEFCGFDAETIGDDIEALADYLNIGEFHLLTHSTGGFAGV
                     RYAMRDSGRLASLILTDTGSSTSVIPGDVETIRKFNDKLACYFEKFNWEQIVANLWAN
                     PFPFFRGIVESPQARDLMRSAQDMISLGDRSDIARFVRSFYTDPDPKIEGLRGIRCPT
                     LIIYGEKDDLFIETSKMMAREIPHAQLLEYPGVGHMTALECPQRLAKDIIEFIKENSR
                     "
     gene            14985..15926
                     /locus_tag="PLF77_03925"
     CDS             14985..15926
                     /locus_tag="PLF77_03925"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HPC08037.1"
                     /translation="MYKKIFLAVLLVIVVSSCAPTHASRYREGSNFNQQTNLTIQDEQ
                     RLTREALPQILKDYPSAKNQELQKYILDLGMKIVRANHLEGNPYHYTFSVVDVADINA
                     FALPAGTIFVTVPLIALASNEAELAGVISHEIGHVVARHTAERMYVMEQAQNKSWMYA
                     TGGAVVGAIVGYGLGTVLCSDGDSACKARAALIGGALGAGGGLLAQKYTFLVNSREDE
                     MEADRIGFKYAIAAGYDKDQVGTFYEKLLEIEKKSGQSGNAIAKNLNDALSTHPPSEE
                     RVRQMRELAAASPVRDAVIHTSQFNRAKQIAKKIGKK"
     gene            15942..16592
                     /locus_tag="PLF77_03930"
     CDS             15942..16592
                     /locus_tag="PLF77_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPC08038.1"
                     /translation="MLTALLTIFVSSFIIALSGAMMPGPLLTVTISESSRHGFMTGPL
                     LIAGHALLEILLLAALLLGLAPFFQMPAVFVVTALAGSVILLWMAFNMFRSLPNVRLA
                     WDAHEKSGNHPVINGILMSVANPYWIIWWATIGLGYILYSRQFGLRGIGFFFAGHILA
                     DLSWYSFVSAAVTRGRHFLNDRLYRGILSLCAVFLVVFACSFLYAGIEKLSVILSG"
     gene            complement(16778..17470)
                     /locus_tag="PLF77_03935"
     CDS             complement(16778..17470)
                     /locus_tag="PLF77_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPC08039.1"
                     /translation="MKKIGFLVVMLMLMAVFTTAQAAEVRAGGLPALAADDSNATSTV
                     NLSREGEVRAGGFPKMDKYDTCNDCTPPQCPPPPPPPPPVEEKVCPPCPECPACPPPP
                     VVEAPKPAIIEKGRQTLDVKFAFDKATIKPGFYKDIDALANVMKDYPDLNVVIEGHTD
                     SVGSDAYNKKLSQERADAVKKYMVDNAGINANRLKAMGYGEEKPIASNSTAEGRAQNR
                     RVEAAVDYVIEK"
     gene            complement(17614..18615)
                     /locus_tag="PLF77_03940"
     CDS             complement(17614..18615)
                     /locus_tag="PLF77_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC08040.1"
                     /translation="MRQYKCSVEVLPDVFCIRLPLPGRKPGPVNVYLFKGRKNTLIDT
                     GMAQTAGILKKALAEHHLGFADIDTIIITHGHPEHFGAARKIVLAGRAIVMAHEGDRT
                     FIEKGYGISAKQYKKFFRITGVPRNLEIILRLLFFFFKRMADPCRVDTIVREDDQIDI
                     GRYRAKILETPGHSRGSVSIYLEQEQTLFCGDTVIKHITPNAFVVPDEDATLPVRLSQ
                     DEFFKSLMKIKSLSPRIVYSAHGKPIDDVDKIIAGYEKAFAERQENVLALIHAGEKNI
                     YRIARRLFPNIGGWRLPLEIILSISEVYSAIQILQKKGKVCLRMRNNLLEVDQYRHP"
     gene            18835..20424
                     /locus_tag="PLF77_03945"
     CDS             18835..20424
                     /locus_tag="PLF77_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPC08041.1"
                     /translation="MDNESLLSFGRRDFLKYALLAAQALTLPSLHGCAIPKKNVPAIV
                     DATFLIKNANLVDVTSGLTVENSWLLVENGTIAAMGTGNIKNNYRGMVFDLQNRFLIP
                     GLIDAHCHATASPVFSMRMTDLPNHARQQKQNFVSAVESGITTIRDMGAFPVLLKRFI
                     RDIEKGNMPGPRVVYCNSILNVMGSHPEIPPSDINFFALPASLFIGMIMNNFKNTKEM
                     EECLEENARGASFIKLTLDNRTIFCKKNKAIPVYSKEQLDIIFNFAGKNGLPVVGHHH
                     FKFGFDRAMAYPFHSIEHLIADRILSDDDVLRMAKNKVAIVPTMTVAQSYLMEEAFDN
                     IPPPYATPDVMDELHVRKNYFGHEAFQHCDPVLHKQNLAALKYYKTIGRDNLWEHKKF
                     LVDPDVYFGMMMNGYANLKKMNQAGILIGCGIDAGMPFCYFGGNYREYEILQRNGFTN
                     IEILRCATISNAKILRLEDKIGSLETGKYADMVVLDKNPLEDIRALRSPCLVFKQGDP
                     MYSAINLQENGIMGRQPAKNT"
     gene            complement(20474..21742)
                     /locus_tag="PLF77_03950"
     CDS             complement(20474..21742)
                     /locus_tag="PLF77_03950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPC08042.1"
                     /translation="MFDYMMTKEQLKIRDEVRDFIKWVPRQMVLDMDTDKIKFPKEFL
                     QEAARRNLMGCRYPKKWGGRDMDWVTTCMVMEEVGTIGYEMACVFGVGAELVCDAIIL
                     HGTDEQKEKYVKPLLRGDLFAAECLTEPRGGSDFFGATTKAEDMGDYYLLNGMKRFIV
                     GAEGADFFLVYAKTHPDAKPQESITCFIVDRTEGVEVKYLYGLMGCRGGGTGRIIFRN
                     VKVPKKNIVGKVQGAYAVFNTMMIPERLGTAAMTIGAARPALEIATNYTSRRKAFGQI
                     IAQFEGVSFQIAEATMLLDAARAMAYVTAKAVDAGADMHRIRRMVSQSKKFITESCQK
                     AVHNSMQVMGGIGYTNVFPIERIYRDIRLASIWTGTSEVMSMISAHEWYREYLMQKAG
                     QKTREHELDAEEAHEEEKIYDDEDMWKKGW"
     gene            22144..22419
                     /locus_tag="PLF77_03955"
     CDS             22144..22419
                     /locus_tag="PLF77_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08043.1"
                     /translation="MLHENEMIMIVIGLTLLVFLVLNYRQLKQTPFFQLLFVSYCLST
                     TGWMMTVLEGFFFEASLNFIEHLCYASSAVLFAVWCWMIPGRTRGNT"
     gene            22416..24437
                     /locus_tag="PLF77_03960"
     CDS             22416..24437
                     /locus_tag="PLF77_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC08044.1"
                     /translation="MIHPVAFLDIIVILAALAAMAGLARRANDYFLISTRVLLACLSF
                     FLLLYGICLAVEWSGISSRFDRIEDYIGALLPMSWAFVFYSFLNELSFRNIQDLNTQL
                     KQRVDLLKEAELRFRTIFDSASDGILIADVREKTFFAANKKLCDMLGYTKEELQKLGL
                     PDIHPEASLALVNEQFDKLSAGEISVAQNIPLLKKDQDVFFSDVSASLMTFDKKKFLI
                     GIFRDITERRKAEEKLLKSEKKFSSIFHLNPNPLAITDVDTGTFVDINQAFVNWTGYS
                     REEIIGFSAHDLHLWVNLKDREKIVNIVTQTGEASSVEIMMRQKSGQIRTVLFSARFI
                     EIEQTRYLLTLAHDISERKQAEEQYRNIFENAQEGIYRATPEGRFILANRSMARILGY
                     DSQEDLITEITDMASQIYLEPQERTKLIQVMEQKGSIKNNEIQFRRKDDRPIWVSQTM
                     QAVRNEKGQIIYYEGIIEDITERKENVERLRRALGGTVKAIATLVETRDPYTAGHQRQ
                     VSDLARAIATEMGLPNDRIEGLRVAATIHDIGKVSIPAEILSKPSKLTNLEFDLIKTH
                     SQSGYDILQDIEFPWPVAQIVCEHHERINGSGYPNHLKGDEILLESRILSVADVVEAI
                     ASHRPYRASLGIDAALAEIEKNKGVLYDAGVVDACLRLFRQKDYQLTER"
     gene            complement(24600..24773)
                     /locus_tag="PLF77_03965"
     CDS             complement(24600..24773)
                     /locus_tag="PLF77_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08045.1"
                     /translation="MLKISKICFAVSGLLLIVDSTLMILNKPNPLGLPLPCPVTLTIL
                     GVGLILFSIAKIK"
     gene            complement(24922..25533)
                     /locus_tag="PLF77_03970"
     CDS             complement(24922..25533)
                     /locus_tag="PLF77_03970"
                     /inference="COORDINATES: protein motif:HMM:NF025050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HPC08046.1"
                     /translation="MIYFDDLLQDDNICDAVSRIAGEFREKHNFPEVRQLGVVVPDVE
                     TAATQLEKRGIGPFFIASDTLKFWNERGEDRTFSGKLGMAIYKGYEVELLEPGIGSAF
                     YKSCVDGKGRMVLQHLGFLVNDVDDYRNKLEKAGCPTWIRGQIKSFPVVIDFVYMDSV
                     AQAGIILEFIDMRFLGIGVKPPGIIYHGLGKLEKITGIRCLKF"
     gene            complement(25648..26862)
                     /locus_tag="PLF77_03975"
     CDS             complement(25648..26862)
                     /locus_tag="PLF77_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HPC08047.1"
                     /translation="MKALLLNLDPLRYVALHALRPLSKKFCYQGPFSTVKLVDIPEPV
                     LPSPEWVKIKTRLCGVCGSDINLMFMKDSPSAMPFTSFPCVPGHEFCGDVVDVGADVK
                     KIKKGDLITAIPALNCETRGIKPVCKSCASGTTANCDNFAEGAFAPGMFTGICKDING
                     GFAEYVIVHQSQARVVPAGVSPESAALTEPLAVGLQAVMDNRPADKDKVLVIGGGVIG
                     AMVVKSIRALDCTCDITVVEPSTFAADYIKQCGANRTMKGGIIEAAVKITKGRAYRPM
                     LGERMVMGGFDKVFDTVGNARTLNLSLRVTATNGTLSVIGIGQEVKLDLTTLWLKLQT
                     VKGCYGYRYNSVASSRKHTFDIALELIASRKIQVDDMLTHQLPLENYREMIEINLQKE
                     TRKAMKTAFCFM"
     gene            complement(26867..28534)
                     /locus_tag="PLF77_03980"
     CDS             complement(26867..28534)
                     /locus_tag="PLF77_03980"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HPC08048.1"
                     /translation="MAKIMGGHLVGKYLKEVENIDIVFGISGGHIEAVLDSFTEYKIR
                     TIDVRHEQAAAMMAHAWSVYTGKPGVCFLTAGPGFANGLTGIANAYLDNAPLVVLCGR
                     HPLRDDLKGALQEINQMDIVHPITKWAATCYDPKRIPEYLSIAFRYAVEGRPGPVFLE
                     LPPDILGVKIEESLAPMPRRTTRKYTVHPDDADLKAAAQIINNAKNPLFIGGSGVRFS
                     GKSDALKAFIEKTGIPFMLMNYGRGELPDSHSHSVMDMGSVGLMFGLPQTDVLIAAGL
                     RFNWLLMSGSLIPAATKVVRIDIDPHEIDRNRMADAGLVGDVGSVLEQLTPLADKKDH
                     SAWSETLKAAGRSLLDYELRIRETATAPIHPVRLVAQIQKVVGDDTVFVTDGGDTNYF
                     GLAFETRQKAGVIAAAAGLFGCLGTGIPFGMAAKLARPDKKVVVLNGDGSFGFNAMEF
                     DTMVRHNIPVVVVINNDCAWGMIKHSQELSIGADRLQCAELGVRHYEKVVEGLGGHGE
                     FVTKDEEIIPALERAFASGKPACVNVMTDPTVTSPATPLFYQALKME"
     gene            complement(28647..29006)
                     /locus_tag="PLF77_03985"
     CDS             complement(28647..29006)
                     /locus_tag="PLF77_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08049.1"
                     /translation="MRFSALLFILYQILKWAAKMKPVYRSYVGSIHQVRIMIKTADGK
                     RGRLFIFDRGTVSSKGGADHPYDAAIVWSDAATGFRVMASKSNEEQFKAAAAGKMKLD
                     GMAFFAQWFNDGVKLVM"
     gene            29144..30202
                     /locus_tag="PLF77_03990"
     CDS             29144..30202
                     /locus_tag="PLF77_03990"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HPC08050.1"
                     /translation="MNQQSRADSIHLISSPTLPYFRSVNGIAILARYVEKYGIHVNTL
                     LTGSGLHPRDLDDPEMFVTPEQELAVMRKILFLVPDPKIGLIIGQQYHVGVQGKLGAA
                     AIFSDTGLEAITMFFKYIALTLTYFQYELHVKDKLAFVNVRELIDLKDLRVFVCEREF
                     VSIYRIVSDILKAPIPVNEIRFAYPKPSYASFYEGVFHCPVKFNAPEHRIIFDSTLLS
                     MPLPMSNPIARKTYEQECKQMCQRLKVKETITDQIRQEILFKEESVPSFTQLSRHLNL
                     SPRTLRRRLTDEKTSYKSLVADIRKKKALDWLKTSDCPLETIATRLGYSDISNFYRAF
                     KRWTGRNPGFYRKEHGRI"
     gene            complement(30326..31705)
                     /locus_tag="PLF77_03995"
     CDS             complement(30326..31705)
                     /locus_tag="PLF77_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC08051.1"
                     /translation="MKTRLDILITDDDRAHRVMLKKLLNGWGYNVTEADDGLVAVEKV
                     RKSSFDLILMDIRMTNVSGIEALEQIKQINADIPVIIMTAYASVETAVQTLKKGAYDY
                     LTKPLDFDELQVSIARATEHSRLKKENEYLKEKLGEKFDRRNMIGQSPSMIKLLEVVE
                     QVAATQATVLITGESGTGKEVIANAIHFNSARKNAPFVKINCAALTETLLESELFGHE
                     KGAFTGADKRREGKFVQADGGSIFLDEVSEMSPAMQVKLLRVLQERELTRVGGHDVLK
                     IDVRVIAASNKDLKKEIRDGRFREDLFYRLNVVTLDVPPLRERSEDIPLMAHAFLKMF
                     AENNAKVIKGFTPQAMQKLASYSWPGNVRELMNAVERAVVLCRTETIGEEDLIFTMAD
                     QALSREVLSAGQKMPAASGNRSLEEIEKQSILEALQSCRGNKSEAARRLGITRKTLRK
                     RLHKYEDGE"
     gene            complement(31910..33589)
                     /locus_tag="PLF77_04000"
     CDS             complement(31910..33589)
                     /locus_tag="PLF77_04000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08052.1"
                     /translation="MMQEKKKKIRWDISIWAAVSAITVLMVILGMMMFMQFQQSETQA
                     QALFIEKGATLIQSFEAGLQDRVDGKNDRFYFQKLLMATAEQPDIDYMIVTDSKGKIL
                     ADSDPSMLDQQYGIDLDTEKIARSKDMHWRQVTNPGGAGTFEIYRGLFPWRQTDAALH
                     HRSLIVFVGFNMDKIEKASQEDIYRTIIRAFILILIGSLAIVSLFLVQTYRLTRASLS
                     RVRVFAEALVRNLPIGLLAVDKDNRIIASNEHARDLFAGDPSNVERQDAGRILPGPLV
                     RLLTKVTMSSGLIEEDVVLSSETKGEQIWEVVVAAFADRDVPEGKILLARNVTAVRGL
                     EKEVAHSRHLNAIGSLAAGVAHEIRNPLSSIKGFAVYFKQRFAGNPDDEETADIMIAE
                     TERLNRVIGQLIEFARPLTLKTEKTALSGLVMQSVKLIEVEARKNGVQVDARVPEDLP
                     AVEIDPDKIKQVLLNIFLNALAAMPDGGRLSVGISQTQAGQEVLISDTGSGIEKNDLP
                     RIYDPYFTSKPAGTGLGLAVVQKIMEAHGAFLNIESQAGAGTKVTLRFAVS"
     gene            33859..34371
                     /locus_tag="PLF77_04005"
     CDS             33859..34371
                     /locus_tag="PLF77_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08053.1"
                     /translation="MKNHYYGQGIYRTAKQLIRKIINRRLLKNPYFDKIHHLLTCLFY
                     EGIEERIIGYLNPLWIINDIGAEELTVLVKIKNGDMSDFLFSAIYFIQGLNIRYFFIF
                     HGGKFFFGSVPDKRLSAFIGKNIFNDIFHGAVFRNDFFVKSNIPQIARVKFNPFGSAE
                     YRTCRMTEDH"
     gene            complement(34609..>35376)
                     /locus_tag="PLF77_04010"
     CDS             complement(34609..>35376)
                     /locus_tag="PLF77_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC08054.1"
                     /translation="IYQIDYQTGRFLKANDVFCKYVGCSQEEVTSLTAFDILTEESKK
                     RFLERVERLSQNLEVPDSVEFEILNKKGESFYIQLQIRNVCDVKGRVVAADVVAHDIT
                     ERKQAEDELKSFAEDLEEANITLRVLMNQRDEDKQEIEEKLQTNMRDLVMPYLRKLKR
                     LNLDGRSKNYLDILESNLNEVLSPFMKNLQSSFNHLTPQEMQIVSLIKKGKKTKEIAE
                     MLNASTKTIATHRNNLRKKLNLRNSKINLRSYLLSSG"
BASE COUNT         8405 a         9508 c         8195 g         9168 t
ORIGIN      
        1 acaagccgct cgccggtgat cgccttcacc tacaacgaac cgacggtttt caccgaatat
       61 ctgacagaca tcgcgcgcaa tgcccggcaa cagggtatcc gctgcgtcat gatcagttgc
      121 ggtttcatga acgaggcgcc gctcgctgaa atgtgcgaaa cgctggccgc cattaaaatc
      181 gatctgaaag gctacagcga ggatttctat cgttcggcct gccaggcgga attgaagccc
      241 gttctaagaa gcatcaggca gatcgccaaa agccgcgcgc atctggagat tgtaaacctg
      301 gtcgtcccca cattgaatga ctcggacaag atgctgaaag gattgatcga ctgggtgatg
      361 ggagaagtcg gccccgatgt gccgttgcat ttttcccgtt ttcatcccga ttaccagctg
      421 cgcaatcttc cgtccacgcc cgttgccacg cttgaacgag cctatcgtat ggcgcgtgac
      481 agaggattgc attatcccta tgtcggcaat gtccccgatc atccgggaaa caacacctac
      541 tgtccgaaat gcggaaagat cgtggttaaa agacaggggt tttttattac cgggatgaat
      601 ctccgcaacg ggctgtgtac attctgcaag aatagaattg ccggcgtttg gacctgaaag
      661 gagaacctac catatgaaat ggctgcgttt gctttgtttt ttgccggtga ttgttttttt
      721 tctgacgtcg gcctgtcagg ccaacaaaga cgccgctgac ggcgtccggg aaccggcttt
      781 tgccggcaag ttctatccgg catccgcaga caaactgcgt ctgaccatcg aaaagtatct
      841 gcaagacgcc ttgccgccca gggtcaaaaa accggtggcc gtcattgtgc cccatgccgg
      901 atatatcttc tccggccaga tctgcgctga cggtttccga caggccggtc atcacccgta
      961 cgatgtcgtg gtcatcctgg gaaccaatca caccagcccg ggcctgcagg gagtttccct
     1021 ctatcccggc gaaggatttc gcacgcctct cggcattgcg gcagtggaca aaaccgtcat
     1081 tgagtccctg ctcacggaat ctcctgtcga ttgcatgctg gacaaatccg cgcatgtctc
     1141 tgaacattcg gtcgaggtga tcgtgccctt tgtccagatt gtttttccca acgctaaaat
     1201 cgttcctgcg gttgtcggga cgcaggacag gcacaccagc atccgcttcg gtcaggcgct
     1261 ggcaaaagtt ttaaaaaaca aaaatgcatt aattgtcgcc agctccgatc tgtcgcacta
     1321 tccctcacgg gataatgccg tcaaggttga ccgccagacg cttgatgcgg ttgccacact
     1381 cgatcctgcc gtcttccaaa aaacgataca ggacacgatg agcaaaaaca ttccgaatct
     1441 ctccacgtgt gcctgcggcg aagcgccgat tatcgcggca ctggctgccg cccgattact
     1501 cggcgccgat cgcgggaacg tcatcagtta tgccaattcc ggcgatgtct ccgtcggcga
     1561 ttccgctcag gtggtcggtt acggcgcggt cgtactgaca gccgggcaaa aggggatttc
     1621 ttcttctcaa acggatctgc agaaaaccgc ggctgtcccc ttcgatctgg gcacagccga
     1681 taaaaaggca ttgctggcct ttgcccgtga aaccctatcc cgttttttta cgaccgacac
     1741 ggttcctctg gcacggcatt tcccagcgca tttgaatcac ccccgcggcg tctttgttac
     1801 gctcaaaaaa aacggcgcct tgcggggctg catcggccat ttaataacgg acgaaccgct
     1861 ggtcaagctc gtgggcgcga tggcgctgca ggcggcattt aatgacggac ggttccctcc
     1921 cttaaccgcc gaggaactcc cggatgtcga catcgagata tccgtgctca ccccgatgaa
     1981 acaggcggcc cgcccctccg acattatggt cggccgggat ggtgttgtgt tgagcaaaga
     2041 cggacgttct gctgttttcc ttccccaagt cgcactggaa cagagctgga gccgcgatga
     2101 catgctggat catttatgca gcaaggccgg actggaaaaa agatgctggc gtcaggacgc
     2161 aacattttca atttttcagg cgatcgtatt caccgaatcg cagctgaaat aaacgcatga
     2221 ccgtttatct tttatccatc ggactgattt ccatcctggg gcaggttatc ctgctcaggg
     2281 aattgaatgt ctctttctac ggtgtcgaac tgatctatct gttcgccttg ggcatctggc
     2341 tgttctggac cgccgtcggc gtgatcgccg gacgacggat ggctcttccg tccaggcata
     2401 gcccggccgt tttgttcatc ctgttcggga tgatcctgtt cctggacgtc gccttcatcc
     2461 gttccagccg tctgatcttc ggcggcgtac cgggcgccta tctcgatttt tttcaacagc
     2521 tccttgttgc gaccctgtcg attctgcccg tgggactttt atccggactt ttatttcagc
     2581 agaccgccgg attgtatgta gactccggcc ggacgctggc agccgcctac gcggtggaaa
     2641 gcgccggcgg tttgatcggc gggttattct ccacgctgtt gattatcttg gacacccaga
     2701 atcttttcgc cgcgctgatc tgctcgatcg tatcgtttgc gacgcctctt ctgggtgctg
     2761 gaaagtccct gggagctttt ttgcggcgga cggcaatcgt gctgcttgca atgtttgcgg
     2821 gcctgatttt caatgcatca cctttgcatg acatctcgac ccgttggaat catccgaatc
     2881 ttctggacag cagcgactcg ccttacggca gaatcaccgt caccagaatg ttcgatcaga
     2941 tttccgtatt cgaaaacgac gccctgtcct ttgaaacgga aggaaccgat gccgaatatt
     3001 tctgccatct ggtaatgctt cagcatcccc atcctcgaaa ggttctgatt ctgggcggcg
     3061 ggatggaagg catcatcggg gaagccctaa aatacggacc ggaccatatt gattatgtgg
     3121 aaattaatcc cgtcatgacg aagcttgtta tggaacacct gccggagtcc atccgtcagt
     3181 cgctccagtc acccgaggtg cgcatcattt atgccgatcc taggcggtat ctcacaaaca
     3241 gcgggaggta tgatctcatc ctggtcggca tgccggaacc gtcatccgga caagccaacc
     3301 gtttttatac acaggaattc tttaaacaat gcgcggcaaa acttaaatcc gggggccttc
     3361 tcggatttaa attgcaaacg gctgaaaatc tctggacggg accgctgatc ggccgcaatg
     3421 caagtatcta tcatgccctg cgttcggttt ttcccgctgt gctttttctg ccgggcacga
     3481 caaacatcgt caccgcatcg tttgcgccgc tgcccgatac accggacatc atgaccggcc
     3541 gcctgcagca tctccaatta gagacccggc tgatttcaga ccattacatc cgctaccttt
     3601 ttaccaatga cagatttgcc gccgtcaaaa atctcctgaa cgaaaaaaag gacttgctca
     3661 attccgatat cagaccggtt tgttatcaat acgcgctgat catctggctg tcgaaatttt
     3721 tccctcgcgc gacgcttttt gatttctcct cgatcatgga gaagaccttg ttgcgatatc
     3781 tgccggtctg tttcttgatg gtcttgcctg tcctctttct ggtcagccgt ttcctgcccg
     3841 gactacgccg caaaatactc gtggcggcgg ccggttttgc aggcatcgcg ctggagacga
     3901 tcctgctgct ttattttcaa ctcaaatatg gcgtcattta tcgggatatc ggtttgttgc
     3961 tgatgagttt tatggcgggc cttgcgctgg gcgctttggc ggttgataaa ctgatggcgc
     4021 gttttgcgga ccatcgccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
     4141 gctgggcgct ttggcggttg ataaactgat ggcgcgtttt gcggaccatc gcctgggaac
     4201 ccggcggtgc ggcattatac ttgtggccgg atcctgctgc ctttgcgcga taaccgccct
     4261 cttaatcaga aacagtctgc ctgccggtct ctggctgacc ggtctcctgc tgactgcaac
     4321 aggttttatt gtttccggga tctttgccta tgccagtctt tatgagattg atgatcagcg
     4381 cgcaatcatt tcacctttgt atgcggccga tctgatcggc ggatgtttgg ggtctgtgct
     4441 tgtcagtctg attttcattc cgctggcggg catggatacc acgatgtggg gaatgcttct
     4501 actaagcgtc tatgccttcc tgctgttgta gccgccggcg cttcaacgct caatccttgg
     4561 catacggcgt ccccgaagca agccatggat ttttttcaga ccatggcggc tgatcgtctt
     4621 tactgaaaag atcgttcccc ataaccagac aatccatgtc cgtctgcatg aagcaccggt
     4681 aggcgtcttc cggcgtgcag accatcggtt cgccgcgaac gttgaagctc gtgttgacga
     4741 gtaggcccca accgcttttt tgcttatacg ccttgatcag cttccagaac cgttcgtttg
     4801 ttcttttgct gacgctttgg attctggcgg aatagtcgat gtgggtcacc gccgggagat
     4861 cggatcgcat gtgataaagc ctttcgtaca gggtcatctg ccgctcattt tccggttgcg
     4921 gcctgcggcg atgttccttc agcccggcaa ccagcatcat gtaaggcgac ggtcggtcga
     4981 gatcgaaata ttcggccacg tcttcttcaa ggacggccgg agcgaacggc ctgaattctt
     5041 cccggtattt tattttcaga ttcagttttt tttgtgtgtc cggcaggcag ggattcgcca
     5101 gaatactgcg attgcccaag gcccgcggac catattccat gcgtccctgg aaccagccga
     5161 ccaccagccc ccggaggatc cgttccgcga caaccgcaca taattcatcg aaatcttcgt
     5221 agtgtacaaa cgatgcctgg tatgttttgg cagtgcgctt gatgtcgtcc gatccgaatt
     5281 caggacccag gaaagcgccc gccatggcgt cctgttccga cacgcttttt ctcggctgtt
     5341 ccatcccgac atgccaagcg gcaaaagccg cacccagcgc accgccggca tcgccggccg
     5401 acggctgtat ccagagatcg tcaaatattt tctggcgcag caacgcgccg ttggctacgc
     5461 aattaagcgc caccccgccg gccataacca gataattact gccggttaat tttcgcgcgg
     5521 tgcgtgccat ccgggagaca acatcttcag tgatctgttg tatcgccagc gccatattca
     5581 tgtaagactg tacgatctca ctttcccgtt gtcggcgggg aattccgaac agtttttccc
     5641 atttttcatc ccggcacatg gtcagaccgg tggcaaaatc gaagtaatcc atattcagaa
     5701 gaatggatcc atcctcccgg atatccacca gatggtccag aatttttctt cggtataaag
     5761 cggtttgccc ggagtcggca tgaccgtacg gggccagccc catgagttta tactcgccgc
     5821 tgttgacttt aaacccgcaa taaccggtga aggcggaata aagcaatccg accgaatggg
     5881 gaaaatcaag ttcccgaaga attttgatgg accggccctc ccccagaccg atggtggctg
     5941 tcgcccactc gccgacgcca tcgatcgtca gaatggctgc cttatcaaaa ggtgacggat
     6001 aaaaggcgct cgcggcgtga gaaagatgat gttccggaaa aagcagtttg acccggcccg
     6061 gaccgatgcc ggcgataccg tcgtgcagca ttttacgcat aaaaattttt tctttcatcc
     6121 agacgggcat tgcggccata aaacttttca gtccccgggg ggcaaatgca tgataggttt
     6181 ccagaagcct ttccagtttc agatagggtt tttcgtaaaa ggccacaccg tcaagatccg
     6241 ccaggtgacg tccgccctcc cgaaggcagt atgttgccgc atggacggga aagacgctgg
     6301 tatgtttttc acgggtaaag cgttcctcgt gagcggcggc cacgatccgg ccgtcaatca
     6361 aaagcgcagc ggcgctgtca tgataatacg ccgatatccc tagaatggca ggcgacatgt
     6421 cttcctctta aaaaagtgta taaatgaaag gcccgacggc tgatccgccc gcgaagatca
     6481 tcaaggctcc gcataaaagc agcatcagaa tgatcggcag aagccagaat ttttttctga
     6541 ctttcaaaaa accccataca tctctgaaaa aatccatgtt tgtctcccgc ttcagtacgg
     6601 atgtttaatg tcatccgccc tgaattcgtg ctcgcgtatt ttgaaaaccg aatgttgatt
     6661 ttttttccat tctttcaact gcagcgtatc gttgccgagg cctcgccgga caagaccgac
     6721 gggcaacaca aaaatcaaaa aaatcaggag caagatcagc cgggacatca cagcgccgag
     6781 gatgtgcgca atgcccagcc agaccatcgc ccacggcttg aatatcctgg gccaaacgat
     6841 attcagaaaa agcagcagca gagcaaaacc gtaaaaaaac gttatgtccg aaaaaaaacc
     6901 tgctatcaga caaagcagcg cggcagccat gccggcatct ttggcctgat cggttgtgat
     6961 tgcgtcggga ataaatttca ttttgtcttt gtaatctttt ctgaaacgta ctttgatttt
     7021 aatatcacaa gtcggtcggg aaaaccactc tcatcatcgt ggcaaaggtt atttcctcac
     7081 cggagattgc cgcaagcggt gatgatccgg aatggacatc cgaaagaatg ttgttggtct
     7141 gttctgaaaa taattcattg cggcatcccc gccaccggtt cgccggatga tgtcagaccg
     7201 ggcaacggtc ggtttgattt tgcaaagacg acggcaaatg cctgtttaga tttccttgat
     7261 ggttcttaat tttttacacc cgccgtccag ccacaggcag agatcctgta aggtcacatt
     7321 gcttgaacat ttaaattgcc gggccccttc cggcaagaat tcccggtcct gcatgctttt
     7381 gtagattttg agcgaaccgt cttcgaacag aactataaaa aatgacatgt ttcacctttc
     7441 gattgcagaa cgacaaacgt ttcttactgt gccttcggac aggggcctat atatttgcct
     7501 ctgatcttgt tggacatcgt catggccggt tgtcctttca ttttaaaact ggtggtcgac
     7561 gcgccgttca ttgccttgtc cgtataggtt gtcgttccgg acgtctgaac gaccccttcc
     7621 ttgcccgtac attccacctg atagctgaca gtgccgccgc tgaccttttg gctgatgatc
     7681 ttacattcaa agtttttgtc tttattctgg ggaacgggat tgcttttcgt gatgcactga
     7741 cgaaacgtcg tgggcggcat ggattgcggc atgcccttca tttccacctg ggaggtgatt
     7801 tcccaaagcc cttccttcag ttcggcaaaa acgacggttg tccaaaaaag acaaacaatc
     7861 atcacaaagg aaaacaatat ttttttcata atgcaaatct ccttgatgtc ctgaattcag
     7921 ccgatgatca tccattcgcc tgttggattc accagccgat caaacatgca acgactgcct
     7981 gttgatgaat gattatactt ttacttccgg tgaatcgcaa tctatttgtt cccgttgttt
     8041 aatttcccgc cggataaccg gtaatatctc tgatgtctct gaacaacggc ttgagccgtt
     8101 cgtacatctt cagatacacc cgcctgtaaa gattgtcgta cagaccgtga ttatccttat
     8161 ccggttcaaa aaccctgccg gtccgggtca tgttggtaac cgcagacgca aaatcggaat
     8221 aaagcttaag cccgaccgcc gcgtcaatgg ccgcaccgag tgcggatgtt tcaaacgtgt
     8281 gaggacgttc cgccgcgatg ccgaaaatgt ccgccgtaat ctgcatggcc gcgtcgcttt
     8341 gcgaaccgcc tccggaaact ttcagccggg taaccggtat tttcgtcttc cgctgggtca
     8401 attccccgcc ctctctcaat ccgtaaacca gcccctccag aagagcccgg taaaggtagg
     8461 cgcggctgtg cacatcgcca aaaccgataa ttgatccctt tgcaaaagcg tccacttccc
     8521 ggccaggcgt ccagtaaggc tggagcatca gccccttgga tcccgccggc acatcctgaa
     8581 tcagggaatc aaagaatttc tccggaggtt ccgctgtctt ctcggcaagc atgcactcct
     8641 gaaggccgaa ctcttccttg aaccagctga ccatccagaa tccccggaag atcgacacct
     8701 cggtataata agtatccgga acggaagcgg gaaacggcgg gatcatcgga tgaagctcaa
     8761 catatttggg cgtggccacg tcaaacgtgg caatggttcc gaaactcaga catccggttt
     8821 ccggcgtgag acaaccggaa ccgaggatct cgcagccctt gtccgatccg gccgcaaaca
     8881 ccggcagccc ctcgggaatg ccggtctctg cggaagcttt tttcgtaatc gcacccatca
     8941 tttcggacgg tttgaccagg cgaggaagct tttctttttc caccttgaag attcgccact
     9001 taaaatcgaa cttgcccgcc cactggtagg tcttattgtc aaacggcaga taaccgacat
     9061 tgttacccgt cgaatcgaca aattcgccgg tgagccgatg cgtaaaaaat ccggacagca
     9121 ccagatattt atgcgttttt tcccagatgt ccggctggtg ctgacagatc cagttcgcct
     9181 cgcattcctg aatgacgttc tccagcgtat ccaggaggtt gactgttttc agaagcggac
     9241 gaagcggcgc cggtaggatt tttctggagt cggcctttcg ctggtccagc cagataatcg
     9301 ccggcctcag cgggttgccg ttctgatcaa cattgaccat cgtggcgcgc tgcgtcgtca
     9361 gcgtgacgcc tttgacatgt tttttcagaa cttcctggtc ctgcaacaac tgatgggttg
     9421 ttttgcaaaa cacatcccag tagtagtcgg gccgctgttc tgcccagccg ggatgttcgg
     9481 aaaaataagg ttgaatgggc gttttgacga tcgccttgat atttccccgg acatcgatga
     9541 gagcggcccg gatcgactgg gtccccgcat ccacggaaag aaccagatcc tgcgttgtat
     9601 ttttttcacc cgacatagtc atctctcctt tttttatgat ttccgcgaat catcgcgctg
     9661 ctttcatcaa tccagtccga gctgaccgcc cggattcatg atgttgcgcg gatcaaaatg
     9721 ttttttgagc gtccgcagaa cgtccaactg ttctttgccc agatgtttct ccatccacgg
     9781 tccgatcatc cgtcccacgc cgtgatggtg actcagcgaa ccgccgtgtt tctgaatgtt
     9841 gtcgataata ccccgctgga atttccgaaa ctcttcggga tctttcatat gcgcaataaa
     9901 gataaaatag agattggtcc cctgcgggta aaagtgagaa gcgtgtgtca tgcacatggt
     9961 ctgcggcctg ttcttgatat acgcgcgcac gccctgatgc agacggtgga gattgtccca
    10021 ggtcacggca gcttccagcg tatcgatcag gatgtcgtaa tcgccaaggt cttcgcgcat
    10081 atacggttcg gtataccggg tgtgctccca tttttttgcc gcgtaaccgg tcagcgatat
    10141 cgccccgtgt tttttcgcaa ccgttttgat tttgcgcacc acaagcttcg tataatcgca
    10201 atcgccctcg gcatttccca gactcagaca gcgctgcatg ggcttgaatc ccttcatcac
    10261 gaggatttta tccacaaaat ccggcatgcc gtagagtttc agcccccggt cggtctcctc
    10321 cggatcggaa atccggtaaa ttgccggctt gccgaattcc gactgcatga tttcccggct
    10381 ggcatttacc gcggcctccc acgtcggata cataaaactg aaacgccggc gattctccgg
    10441 catgtagcgg aaaattttca tggtgacttc caccagaata ccgaatgatc cttcagaacc
    10501 cttcatgatg tcattcacct tgggaccggt tgcggtcgcc ggataatcca gcgttttaaa
    10561 cgtcccggcg ggtgtgacat attcctgact gaaaacaata tcataggcat caccgtaata
    10621 ggtggaggcc tgtccggacc ccagtgtgac aacccacccg cccacagtgg aatattcgaa
    10681 ggattgcgga aaatgtccgc aggtgtaacg atgttttgtt ttaaacagtt cgggcgcttt
    10741 attgagaccc tcttcataag ccggaccgaa catccccggc tgaacgcgcg cagtctgatt
    10801 gaactcattg atctccagca gtttattcat gtgggtgctg accaccaggg aaacgccgcc
    10861 cctgacggga cgacaaccga agtttacgga agaaccggcg ctgtaaaccg ttatgggaat
    10921 gttttcctga tgacagtatt gaacaatttt ctgcacatcc tgtttgtcac gcggatgcac
    10981 caccacgtcc gcgacttccc ggataatccc caaacgcagt tccagcatct cttccgtggt
    11041 tttgcctgat gcgtatttca cacggctata atcatcgagt gcaacatttt cgccgcccac
    11101 aatattccgg aggcaatcca tctgttgcgg tttgagtgcc tgggcacggt ttaacgaaac
    11161 cggttcaagt ccttctttta ctcttgaagc gaagtccttg tcgtttaaat gaaattcttc
    11221 cttgatcatc ctgtaccagg catcgctggg atgtttgaaa ctgtgaatat ctccgtattt
    11281 aaaaatggat ctgtacgttc cggctgccgg tgccttttcc gtccagtttg gccggaaata
    11341 atgagatatc atcaccatgt cctccttttc tcttgaactc ttttcatgtt cattcaattt
    11401 tttaatcaac aggtatcttc agcagattga cgaccgcttc cacttccttt tccattctct
    11461 gctgatccca gtgcaattca tcagcggcaa cctgtgcaac tttacgcaac acatcgtcac
    11521 cgggattgcc cagtgtgccg atacctgtac ggcgcatgac aatatcggcc agggtttggg
    11581 ccatttcatc acgaacggca tacacaacct gcgccataat ttcgccgtcc tcgtttaatg
    11641 tctcggacag actttttttc tcacgcgcaa gcccgacgac gtcccggtac accgtaccgt
    11701 aattgcgcgc cagatattcg agggtggctc cgctcaatcc atcagccgga cgcttcgctt
    11761 cctcaataaa aagacacaga tctttgatat cgcagcccgt cagatattgt ttgtccgtaa
    11821 cactccggcc aagatttctt cccatttttt tggcgacgat cgccagacag ttttcggcca
    11881 gtttgcggct ggtggtgtat ttaccgcctt ccacggtaat cagtccgtcc aatccgtcat
    11941 ctttattatc gtagatttca tatttgcggg aagaagaata ggtttcgctg gtttcttttt
    12001 caataagagg gcgcagcccg ccgtaggtat gtttgacatc cgcatagctc agtttgccgt
    12061 cgccgaagga gctgttcacg tcagcaatca gttccatgat gctttctttg gtgacgcggt
    12121 agtcgtccgg attaccgtta aacggtttgt ccgtcgtgcc gatcagtgcg tgattgcgcc
    12181 acggaatcaa aaagaaatga cgtccctccg gcgtcatcga cccgattgta tagttgccgg
    12241 tcagcagttt cttagacgtg atgatatgga tcccttcgga ccgccgaagc gttgcgttga
    12301 cctgaccatc cggtttggcc atgcccagga cgatatccgc ccagggtccg ccgcagttga
    12361 tcgttacggt tgcgggcaca ttgtgcatct taccgctgaa caaatctttc acctttacac
    12421 cgagaacatg ctttgcgccg tccatgagaa aaccttccac tctcgcgtaa ttggctgccc
    12481 tggcaccgtg ggcgacggcg gatttgataa aggccaacgt cagcctttcc gggaaaagac
    12541 tggtacagtc gtaaaaaata gaagcgccgg tcaatccttc agcgcggacg tgaggctcat
    12601 cctggagaac ttgccgggcc gaaatcgttc tgtggacggg aatttttttg cagggatccc
    12661 atgtgatatt tttgtcgtag gacagaacgt catagacgag catacccact ttcaccaccc
    12721 acttattatt tttcagcggc tttttgtaat gcgtcatcat catcggccac ggatagacaa
    12781 aattcggagc aatattctca agaacacgcc gttcgcgcag cgactcgcgg accagtccga
    12841 cttcaccgtt taccagatat cgcaaaccgc cgtgaatcat tttggatgtc accgccgatg
    12901 tcgcccagga aaaatcattt ttttccagga gagcgacttt cagcccccgc gtggctgctt
    12961 cataagcgac cgacgcaccg ctgatgcctc cgccgattac agcaacgtca aatttctcac
    13021 ctgtgtaatt ttcaataaat cttttcatga ccatcactcc ttatcaaacg ttggtctttt
    13081 aattttattt ttcaccgctt cctccgcgta aataagaacg tctctcatga tgttgcgggc
    13141 gtcatcagcc tttttcctcc tgatggcttc aaatatttca tcgtgaaatt gccgggaacg
    13201 ttcgacattt tccggatcgt taaaataaag atgtccgtaa tcgcggaaga tctgattgaa
    13261 aaaattgagc ccgatggtac acagcagatt atgcgtgcag cgggcgatct gctgatgtat
    13321 ccggatatct ttttccagca tggttatatt tttttcagaa aggagctgct tcagatcgtc
    13381 gacgtccgat gaggtgcgcc tttgcgccgc cagataagcc atctgcggaa ccagataacg
    13441 gcgcgcctcc agaatattga agataatttc cgtccctccg tccatcttta cagccgcctt
    13501 gattaaatcg aaattaccgc tgttgagata atctttgaca tagagcccgt cgccatgacg
    13561 gatttccaga agatcgatat tctccagttt tctcaatgct tcgcgcacgg tggcccgatt
    13621 gacattgaaa ttttccgcca tctctctttc cgtgggcaac ttttctcccg gcgcgatctg
    13681 accggacatg atctgctgct ggatgacggt gacaatttct tcgtgaagac gtgaacgcgt
    13741 aacaggacgg gcaaaaacct ttcgggtcat cgtagttgtc tctcctcgcc ttcggcctta
    13801 ttggccgggt ggttcgacca ataacacatc gcagattctt tgtcaatcat ttatttttga
    13861 tataatcttg aaaataacgg cttcagttgc ccttccgtta atcccggatt ggccggcaga
    13921 tggtatcgac ggcaaaaaaa tgctcttgtc aagacagatt acaaatcaaa tgtttatcgg
    13981 ctgttttctt tgatgaattc gataatgtcc tttgccagac gttgaggaca ttccagcgcc
    14041 gtcatatgtc cgactccggg atactccagc agttgcgcat ggggaatctc ccgggccatc
    14101 attttgctgg tctctataaa tagatcatcc ttttcaccgt aaatgatcag ggtcgggcaa
    14161 cgaattccgc gcaacccttc tatcttcgga tcaggatccg tgtaaaatga tctgacgaac
    14221 cgggcgatgt cggaccggtc acccagactg atcatgtcct gagctgaccg cataagatcg
    14281 cgggcctgcg gtgattcgac aatccctcga aaaaaaggaa acggattcgc ccacagattc
    14341 gcgacaatct gctcccagtt gaacttttca aaataacagg ccagtttatc attgaacttt
    14401 ctgatcgttt cgacatcacc gggtatcacg gacgtcgagg accccgtatc cgtaagaatt
    14461 agactggcca gcctgccgga atcgcgcatg gcataacgca cgccggcaaa accacccgtg
    14521 gaatgcgtca ggagatgaaa ttccccgata ttcagataat cggccagcgc ctcaatgtcg
    14581 tctccgatcg tttccgcatc aaaaccgcag aattctcctt caacgaccgt cttcccgtga
    14641 ccccgcatat ccatagggat aaaacgatat tttttgataa tctcctccgc cgtcgttttc
    14701 cagtaggtgg tgttttctat gagtccgtga acggcgatta tcgggtctcc ccatccatca
    14761 tcttcaaaat aaattgttgc attgccgcgc tttaaaaaag gcatgtccgt gtctccataa
    14821 ttttcgtcat ttcatcagaa gcataaaaca tttaaaacaa atttttctcc tggtcaatgc
    14881 attatatccg cccgatcgca accttgacag aacgtatccg gcatggttat ctaagatcag
    14941 agcggatgat cagagaatat cattaccgcc cggggagata taaaatgtat aaaaaaatat
    15001 ttttggccgt cctgttggtc attgtggttt cgtcctgcgc accgacccat gcctcgcgct
    15061 atcgtgaagg ctccaatttc aatcaacaaa caaatttaac catacaagat gaacaacgcc
    15121 tgacacggga agcgcttccc cagattttga aagattatcc gtcagccaag aatcaggaac
    15181 tccaaaaata tatcttggat ctgggaatga aaattgtccg tgccaaccat ctggaaggca
    15241 atccctatca ttacacgttc agcgtcgttg atgtggccga tatcaacgct tttgcactgc
    15301 ccgcgggaac gatcttcgtc accgttcctc tgattgccct tgcctcgaat gaagcggaac
    15361 ttgccggcgt gatctcgcat gaaatcggtc atgtcgttgc ccggcatacc gccgagagaa
    15421 tgtacgtcat ggaacaggcc cagaataaat cctggatgta tgcgaccggc ggcgccgtcg
    15481 tgggcgccat tgtcggttac ggactgggca ccgtactttg cagcgatggc gattctgcct
    15541 gcaaggcgcg agccgccctg atcggcggcg ctctgggggc cggcggcgga ctgcttgcgc
    15601 agaaatacac gtttctggtc aattcacgag aagatgaaat ggaggccgac cgtatcggtt
    15661 tcaaatatgc catagcagcc ggatatgaca aagatcaggt gggaacattt tatgaaaagc
    15721 ttctggagat agaaaaaaaa tccggccaaa gcggaaatgc catcgcaaaa aatcttaacg
    15781 atgctctgag cacgcatcct ccaagcgaag agagagtccg tcaaatgcgc gaactggctg
    15841 ccgcaagtcc cgtcagagat gccgttatcc atacgtcgca atttaacagg gcaaagcaga
    15901 tcgccaaaaa aatcggtaaa aaataaaacc atgcaaagcg tatgttgact gcccttctca
    15961 ccatctttgt ttcatccttt attatcgcct tatccggcgc gatgatgccg ggaccgcttt
    16021 taacggtgac catcagcgaa agctcccggc acgggttcat gaccggcccc ctgcttattg
    16081 ccggccatgc ccttctggaa atactgctgc tggccgccct gctgctggga ctggcgccgt
    16141 tcttccagat gcccgctgtt ttcgtcgtca cggcgctggc cggctcggtt atccttttat
    16201 ggatggcgtt taacatgttc cgttctctgc ctaacgtgcg ccttgcatgg gatgctcatg
    16261 aaaaatccgg gaatcatccc gttataaacg gcatcctgat gagcgtggcc aatccttact
    16321 ggattatctg gtgggcgact atcgggctgg gatacattct gtattcccgg caatttggtt
    16381 tacggggaat cggttttttc tttgccggac atattctggc ggatttgagc tggtattctt
    16441 ttgtctctgc cgcagtcacc cgcggcagac attttttaaa cgaccgcctg tatcggggca
    16501 ttctgtcttt gtgcgctgtt tttcttgtgg tgtttgcctg ttctttttta tacgcgggaa
    16561 ttgaaaagct aagcgtgatc ctttccggat aatcttccgt ctgcccgcag aaaaaccagg
    16621 cggccgcaac agctcatttc atcttctccg cgcttggatt ttctttcatg ctcttcagag
    16681 cgcgtcaacg gatattttgc actcccccac cgaacacata aaaaagccct gcaggaaact
    16741 gcagggcttt tgatccaatg catggaaaac tgcttttcta tttctcgatc acatagtcaa
    16801 ccgctgcttc aacacgacgg ttctgtgcac ggccttccgc ggtgctgttg gaagcaatcg
    16861 gtttttcctc gccgtatccc atcgctttga ggcgattggc attaattccg gcgttgtcga
    16921 ccatgtattt tttgacggca tcagctcttt cctgagaaag tttcttgttg tatgcgtcgc
    16981 tgccgacact gtcggtatgg ccttcaatca caacgttgag gtccggataa tctttcatca
    17041 cattagccaa ggcatcaatg tccttgtaga aaccgggttt gatcgtggct ttgtcaaaag
    17101 caaatttcac atccagggtc tgtctgcctt tttcgataat tgcaggtttg ggcgcttcaa
    17161 cgaccggagg aggaggacat gcagggcatt ccgggcacgg aggacatact ttctcttcta
    17221 ccggaggagg agggggagga ggaggcggac actgaggagg agtacaatcg ttgcatgtat
    17281 catatttatc catcttgggg aaaccaccgg ctctgacttc tccttcccgt gacagattca
    17341 cggtgctggt tgcattacta tcgtctgcag ccaaagcggg taaaccaccg gctctgactt
    17401 ctgccgcctg agccgtcgtg aagacagcca tcaacatcag cataactact aaaaatccaa
    17461 ttttcttcat aatgttctcc tttcgcactg caattataat aattttttta aatgatctct
    17521 tttaatattc tattgcatat caattatttg ggtaatttat actaaactca atattttatg
    17581 tcaatataat atttattaaa tttttccggc tcattatgga tggcggtatt gatccacttc
    17641 caaaagattg tttctcattc tcagacagac ttttcctttt ttttgaagaa tctgaatggc
    17701 gctataaacc tctgaaatag aaagaattat ttccagcggt agccgccacc cgccgatgtt
    17761 cgggaaaaga cgtctcgcaa tgcggtaaat atttttttct cctgcatgaa ttaaggccaa
    17821 aacattttcc tggcgttcag caaaagcttt ttcatatccg gcgatgattt tatccacatc
    17881 gtcaatgggc ttcccgtgag cggaataaac gatccgcggg gaaaggcttt tgatcttcat
    17941 aagcgatttg aaaaattcat cctggctcaa acggaccggc agcgttgcgt cttcatccgg
    18001 aacgacaaaa gcattcggcg tgatgtgctt gatcaccgta tcgccgcaga aaagcgtttg
    18061 ttcctgttca agataaatac tgacggatcc cctggaatga ccgggcgttt ccagaatttt
    18121 tgcccggtat cggccgatgt cgatttgatc gtcttcgcgc acaatggtat caacccgaca
    18181 gggatctgcc atccgtttga aaaagaaaaa cagcagcctc aaaataattt ccaggttgcg
    18241 tggaacaccc gtgatgcgga aaaatttttt atactgcttt gcggatatac cgtatccttt
    18301 ttcgatgaac gttctgtctc cctcatgcgc cataacgatt gctcggccgg caaggacaat
    18361 ttttcttgca gccccgaaat gttccggatg tccgtgtgta atgatgatgg tatcaatatc
    18421 ggcaaaacca aggtgatgct ccgccagcgc ttttttcaga atgcctgccg tttgagccat
    18481 gcccgtatcg atcagggtat ttttacgtcc cttgaaaaga tagacattga cgggccccgg
    18541 cttacgaccg ggcagaggaa gtctgatgca gaagacatcc ggcaaaacct caacgctgca
    18601 tttgtattgt cgcatcagac tccttcttct tacacaatga tctttccgac aggcgattgc
    18661 gcaaaacacg atagggtcat ttttttgaaa gtataaaaag aaccatgcag cctgtcaacc
    18721 aaagatagtg tatgcaactt cctgccggag aaaagaacct tttaatatat cttgacacga
    18781 aaaacccgcc gattataatt gcttcaatat tattggttgt cacaacggtc acgcatggat
    18841 aacgaatcgc ttctatcatt tgggcgcagg gattttctca aatatgccct gctggcggca
    18901 caggccctca ccctgccctc tctgcatggt tgcgccatac ccaaaaaaaa tgtgccggcc
    18961 attgttgatg caacttttct catcaaaaac gccaacctgg tggatgtgac gtcaggactg
    19021 accgttgaaa actcgtggct ccttgtggaa aatggaacca tcgccgcgat gggaaccgga
    19081 aacatcaaaa ataattatcg cgggatggtt tttgatcttc aaaacaggtt tctcatcccg
    19141 gggctgatcg acgcgcattg ccatgccaca gcctctcccg tcttttccat gcgcatgacg
    19201 gaccttccga accatgcccg ccagcaaaag cagaatttcg tttccgccgt cgaatcgggc
    19261 atcacgacca tccgggacat gggcgctttc ccggttctgc tgaagaggtt catccgggat
    19321 attgaaaaag gaaacatgcc cggtccccgc gttgtgtact gcaattccat tctgaacgtc
    19381 atgggatcgc atcctgaaat tccgccttcg gacattaatt ttttcgccct gccggcttcg
    19441 ctcttcatcg gcatgatcat gaataacttc aaaaacacca aagaaatgga agaatgtctt
    19501 gaagaaaatg cccggggcgc ttcattcatc aaactgacgc tggataatcg gacaattttt
    19561 tgtaaaaaga acaaggccat ccccgtgtac agcaaggagc aactcgatat tatctttaac
    19621 tttgcgggga aaaacggcct tcccgtggtc ggacaccatc atttcaaatt cggcttcgat
    19681 cgcgcgatgg cctatccttt tcactccatc gaacatctga ttgccgacag aattctttcg
    19741 gatgacgacg tattgagaat ggcaaaaaat aaggtggcca ttgttcccac catgacggtt
    19801 gcccaatcct atctcatgga agaagctttc gacaacattc cgccaccgta tgcaacaccg
    19861 gacgtcatgg acgaattaca tgtgagaaaa aattattttg gccatgaagc attccagcac
    19921 tgcgatccgg tgctgcacaa acagaatctc gcagccttga aatattataa aacgatcggc
    19981 agggacaacc tctgggagca caagaaattc ctcgtcgatc ccgacgtcta tttcggcatg
    20041 atgatgaacg gctacgccaa tctgaagaaa atgaatcagg cgggaattct catcggctgc
    20101 ggcattgatg cgggaatgcc tttctgctat ttcggcggca attaccggga atatgaaatt
    20161 ctgcagcgca acgggtttac caatatcgaa atcctccgct gcgccaccat cagcaatgcg
    20221 aaaattcttc gtctggaaga taaaatcggc tcgctggaaa ccgggaaata cgcggacatg
    20281 gtggttctgg acaaaaaccc tttggaagat atccgcgcgt taagatcgcc ctgcctggtt
    20341 ttcaagcagg gcgatccgat gtattcagcg atcaatcttc aggaaaacgg gatcatgggc
    20401 cgtcaaccgg cgaaaaatac ctgatacagc agcgatcaac aaaacaggat ttcgtcatcc
    20461 ggcggcggac gttttaccat ccctttttcc acatatcctc atcatcgtat atcttttctt
    20521 cttcatgggc ttcttcggcg tccagttcat gctcgcgagt tttctgtccg gccttctgca
    20581 tcagatattc ccggtaccac tcatgcgcgc tgatcatgga catcacctca ctggtgcccg
    20641 tccagatcga cgccagccgt atatcgcggt aaatgcgttc gatgggaaac acgttggtgt
    20701 acccgattcc ccccatgacc tgcatggagt tatgaaccgc tttctgacag gattcggtga
    20761 taaatttctt gctttgtgaa accatgcgcc ggattctgtg catatccgca cccgcatcca
    20821 ccgccttcgc ggtgacatac gccatcgccc gcgcagcatc cagaagcatg gtcgcttcgg
    20881 caatctgaaa gctgacgccc tcgaactgcg caataatctg gccgaacgcc ttgcgtcgcg
    20941 acgtataatt ggttgcaatt tccagagcgg gacgggcggc gccgatggtc atggccgccg
    21001 tgccgagcct ttccggaatc atcatcgtat tgaaaacggc atacgcgccc tgtactttcc
    21061 ccacaatatt tttcttgggc acttttacat tgcgaaagat gatccgcccg gttccgccgc
    21121 cgcggcaccc catcaggccg taaagatatt tgacttctac gccttccgtc cggtctacga
    21181 taaagcaggt gatcgactcc tgcggcttgg catccggatg cgttttagca tacaccaaga
    21241 aaaaatccgc tccttcggca ccgacaataa atcgtttcat tccgttgagc agataataat
    21301 cgcccatatc ttctgctttc gtcgttgccc cgaagaaatc tgaaccgccg cgcggctccg
    21361 tcagacactc ggctgcaaac aaatcgcctc tcaaaagcgg tttgacgtat ttttctttct
    21421 gttcatccgt gccgtgcaga ataatcgcgt cgcagaccaa ttccgcgccc acgccgaaga
    21481 cacaggccat ttcatagccg atcgtcccca cctcttccat aaccatgcag gtggttaccc
    21541 agtccatatc ccggccgccc cattttttcg gataacggca ccccatgaga tttcgccgtg
    21601 cggcttcctg aagaaactct ttagggaatt taatcttgtc cgtatccata tccagaacca
    21661 tctggcgcgg aacccacttg ataaagtccc gcacctcgtc cctgatcttt aactgttcct
    21721 tggtcatcat ataatcaaac atgccattct cctttcctgt tgatcattgc gcagagatac
    21781 tttttacgga ttataacaga aatcaccttg aaaccaaaag acacaatgct aaaaaatctt
    21841 accgtctttt tttgccgtct tcatgcttcc cgatggcttg ccgatatgta aacttcatca
    21901 tgattcttag tcataccggt tttcacagcg cccttctcgg tcataatcct cttgacacaa
    21961 cggatggtgt tttttataat tcatatcatg ggatgatcat tcttatccac gtgttcccat
    22021 gcccggtatc gaaccgggca acaacgtcat aataaattca ggggcctcaa aaaagatgta
    22081 atagaatctt caatacgata actatccaaa aagcataagc atttctgaaa aagggaatca
    22141 aagatgctcc acgaaaatga aatgatcatg atagttatcg gcttgacgct tctggttttc
    22201 cttgtcctga attatcgcca actcaaacaa acgcctttct ttcaactgct ctttgtcagt
    22261 tactgtttaa gtacgacagg ctggatgatg accgtcctgg aaggattttt ctttgaagca
    22321 tctctcaatt ttatcgagca cttatgctat gcctccagcg ctgtactttt cgccgtttgg
    22381 tgctggatga tcccaggcag aacaagggga aacacatgat ccacccggtg gcatttctgg
    22441 atattattgt gatcctggcg gcattggcgg ccatggccgg attggccaga cgagccaatg
    22501 attattttct gatcagcacc cgggtgttac tggcatgcct ttcttttttt ctgctgctgt
    22561 acgggatttg tctggcggtt gaatggagcg gaatttcttc ccggtttgac agaattgaag
    22621 actacatcgg cgccctgctc ccgatgtcgt gggcttttgt cttttattcc tttttaaatg
    22681 aactcagttt cagaaatatc caggacctca acactcagtt gaagcaacga gtcgatttgc
    22741 ttaaggaagc cgaattgcga ttcagaacca ttttcgattc ggcgtccgac ggaattctta
    22801 tcgccgatgt ccgtgaaaaa accttttttg ccgccaataa aaaactttgt gacatgctgg
    22861 gctatacgaa agaagaattg cagaaactgg gccttcccga cattcatccg gaagcatccc
    22921 ttgccttggt caatgaacaa tttgacaaat tgtcggcagg agaaatttcc gttgctcaaa
    22981 acattccact gttgaagaaa gatcaggacg tcttcttctc ggatgtcagc gcatcactca
    23041 tgacgttcga taaaaaaaaa tttcttatcg gcattttcag agatatcacc gaacgaagga
    23101 aagcggagga aaagctcctc aaatccgaaa aaaaattttc ttccattttc catctcaacc
    23161 ccaaccctct ggccattacc gatgtcgaca ccggaacctt tgtcgatatc aatcaagcct
    23221 tcgtcaactg gacgggatac tcccgtgaag aaatcatcgg tttttccgct cacgaccttc
    23281 atttatgggt caatttgaaa gaccgcgaga aaatcgtcaa cattgtgacg caaaccggag
    23341 aagccagcag cgtagaaatt atgatgagac agaagagcgg tcagatccgg actgtgcttt
    23401 tttccgcccg gttcatagaa atcgaacaga cgcgctacct tctgacgctt gcccatgaca
    23461 ttagcgaacg caagcaggca gaggaacaat atcgaaacat ctttgaaaat gcacaggaag
    23521 gcatttatcg agcgacaccc gaaggacgat tcattttggc caatcgctcc atggcaagaa
    23581 tcctcggata cgactcccag gaagatctta taaccgagat aaccgatatg gcctctcaaa
    23641 tctacctgga acctcaggaa cgcacaaaac ttatccaggt aatggagcag aaaggctcca
    23701 ttaaaaataa cgagatccag tttcgcagaa aagacgaccg ccccatctgg gtgtcccaga
    23761 caatgcaggc tgttcgcaat gaaaaaggac agattatcta ttacgagggg attatcgagg
    23821 atatcacaga gcgcaaagaa aacgttgaac gcctgagaag agctctcgga ggaacggtca
    23881 aagccatagc caccctggtg gaaacaagag atccctatac ggcgggccat cagcgccagg
    23941 tgagtgatct tgcccgcgcg atcgccacag aaatggggtt accgaatgat cggattgaag
    24001 gattgcgtgt ggcagctacc atccatgaca taggaaaagt ttccattccg gctgaaattc
    24061 tgagcaagcc gagcaaactg accaatctgg aattcgatct tatcaaaaca cattctcaat
    24121 ccggatatga cattttacaa gacatcgaat ttccctggcc cgttgcacaa atcgtttgtg
    24181 aacaccatga aagaatcaac gggtccggtt atcccaacca cctgaaaggc gatgaaattc
    24241 tgctggaatc acgcattttg tccgtggcgg acgttgtcga agccattgcc tcgcatcgtc
    24301 cctatcgcgc ctctctgggt attgatgcgg cacttgcgga aattgaaaaa aacaaaggcg
    24361 ttctttatga tgccggcgtt gtagacgcct gcctcagatt attccggcag aaagattatc
    24421 agctgacgga acgttaaaaa aaattatccc aacgatctca aatcgcgatg ttcctatccg
    24481 gaaaccgtct gctgcaggaa acattaagca tactatttcg acggcgcatt ttggtccaag
    24541 ccagacagat cgcaaccaat acggttattt ttcaatacat ggtcgaacag aggaagcgat
    24601 tattttattt tagcaatgga aaacaaaatc aatcccacac ccagaatcgt cagggtcacc
    24661 ggacacggaa gcggaagccc gaggggattg ggtttgttta aaatcattaa ggttgaatcc
    24721 acgatcagaa gaagtcccga tacggcaaaa catattttgg atattttcaa cataacgacc
    24781 tccttttcat ccatattatt ttaaattttc cgccggatag catgcggcta acgttcatgg
    24841 gatagaattt aaccggcgct ttgattaccg tcgtcttttt tatcgccgga caaacaatcc
    24901 gggatgccgt catgggttct ttcagaattt caggcatctt atgccggtga ttttttccag
    24961 ttttccgagg ccgtggtaga ttattccggg aggcttgacg ccgataccca gaaaccgcat
    25021 atcaatgaat tccaggataa tgcccgcctg cgcaaccgaa tccatataga caaaatcgat
    25081 gaccacggga aaggatttaa tctgccctct gatccatgtc ggacaaccgg ccttctccaa
    25141 tttgtttctg taatcatcca catcatttac cagaaagccc agatgctgca ggaccatccg
    25201 gccttttcca tccacacatg atttatagaa ggccgatccg atacccggct ccagaagttc
    25261 gacctcatat cctttatata tggccatgcc gagtttgccg ctgaacgttc tgtcttcccc
    25321 gcgttcgttc cagaatttga gtgtatccga agcaataaag aaaggcccga tgccccgttt
    25381 ctccagttgc gtcgcggcgg tttcgacatc cgggacaacc actcccaact gacgaacttc
    25441 cggaaaattg tgtttttcac gaaactcccc ggcaatccgg gatacggcgt cacaaatatt
    25501 atcgtcttgt aacaaatcgt caaaatatat catgtctcac tcccctttat tcaatgagac
    25561 aggtcaaaat tttcaagcct tcattacatg tgcttttcag gacactcctt gcctcgcggc
    25621 gccgggcaat aacctgctta cggcttttta cataaaacag aaagcggttt tcatggcctt
    25681 gcgggtttct ttttggagat tgatctcaat catttcccgg tagttttcca acggcaattg
    25741 gtgcgtgagc atgtcatcca cctgaatttt ccggctggcg atcagctcaa gggcgatgtc
    25801 gaacgtgtgt ttcctgcttg aagcgacaga attgtagcga taaccgtaac agcctttgac
    25861 ggtctgcagt ttgagccaca atgtcgtgag atccagtttg acctcctggc cgatgccgat
    25921 aacggaaagc gtgccgttgg ttgccgtgac gcgaagcgac agattcagtg tgcgcgcatt
    25981 gcccaccgta tcgaaaacct tgtcaaagcc gcccattacc atgcgttcac ccagcatggg
    26041 tctgtaggct ctgcctttcg taattttcac agcggcctca ataatcccgc ctttcatcgt
    26101 ccgattggcg ccgcattgct ttatataatc cgccgcaaac gttgacggct cgacaaccgt
    26161 aatgtcgcag gtgcagtcca gcgcccggat gcttttgacc accatcgcgc caatgacccc
    26221 gccgccgata accagcacct tgtctttgtc tgccggcctg ttgtccatta cagcctgcaa
    26281 ccccaccgcc agcggttccg taagcgctgc cgattccggc gacacgcccg caggcacaac
    26341 acgggcctgg ctttgatgca caataacata ttcggcaaat ccgccgttga tgtctttaca
    26401 aataccggta aacataccgg gcgcgaacgc gccctcggca aaattatcgc aattggccgt
    26461 tgtcccggat gcgcagcttt tgcacaccgg tttgattccg cgtgtttcgc agttcagcgc
    26521 gggaatggcc gtgatcagat ctcccttttt tatttttttc acatccgcgc cgacatcaac
    26581 gacatctcca cagaattcat ggcccggcac gcaaggaaac gaggtaaacg gcattgccga
    26641 cggagagtct ttcatgaaca tgagattgat atcgcttccg cacacgccgc agagccgggt
    26701 tttgatcttc acccattccg gagagggaag aaccggttcc ggaatatcaa ccagcttcac
    26761 cgtcgaaaac ggtccctgat agcagaattt tttgctcagg gggcgaagcg catgcaacgc
    26821 cacataccgc aagggatcaa gattcagcaa cagcgctttc atatgtttac tccatcttga
    26881 gcgcctgata aaacaacggt gttgccggac tcgtcaccgt cgggtccgtc atcacgttca
    26941 cgcacgccgg cttgccggag gcaaacgcac gctccagcgc cggaatgatt tcttcgtctt
    27001 tcgtgacaaa ttcgccgtgt ccgccgaggc cttccactac tttctcgtaa tgccggacgc
    27061 ccagctccgc gcactgaagg cggtcggcgc cgatggagag ttcctggctg tgcttgatca
    27121 taccccaggc gcagtcgttg ttgatgacca ccactacggg gatgttatgg cgcaccatcg
    27181 tgtcgaattc catggcgttg aaaccgaacg aaccgtcgcc gttgagcacc accacttttt
    27241 tatccggccg cgccagtttg gccgccatgc cgaagggaat gcccgttccc agacatccga
    27301 aaagccccgc ggcggcggcg atgacgcccg ccttctgccg ggtctcgaaa gcaaggccga
    27361 aataattcgt atcgcctccg tccgtaacaa agactgtgtc gtcgcccacg actttctgaa
    27421 tctgcgccac cagacgaacc ggatgaatcg gggctgtcgc cgtctcccgg atccggagtt
    27481 cgtaatccag aagcgagcgg ccggcggctt tcagcgtttc ggaccacgcg ctgtgatctt
    27541 ttttatccgc cagcggcgtc agctgttcga gcaccgatcc gacatcgccc accagccccg
    27601 cgtccgccat gcggttacgg tcgatttcgt gcggatcgat atcgattcgc actacttttg
    27661 tcgcggccgg aatgaggctg cccgacatca aaagccagtt gaacctcagt cccgcggcaa
    27721 tcagcacatc cgtctgggga aggccgaaca tcagaccgac gctgcccatg tccatcaccg
    27781 aatgggagtg actgtccggc aattctccgc gtccgtaatt catcagcata aagggaattc
    27841 ccgttttttc gataaacgct ttgagcgcgt cggattttcc gctgaagcgc acgccgctgc
    27901 cgccgataaa cagaggattt tttgcattgt tgatgatctg tgcagcggct ttcaaatcgg
    27961 cgtcgtccgg atgcaccgtg tatttgcggg tggtacgccg gggcatcggc gccagcgatt
    28021 cttcgatctt aacacccaga atatccggcg gcagctccag aaataccgga ccggggcgtc
    28081 cctccaccgc gtagcggaag gcaatggaaa gatattcggg aatccgtttg ggatcataac
    28141 aggtggccgc ccatttggtg atgggatgca cgatgtccat ctgattgatt tcctgcaggg
    28201 cccctttcaa atcgtcgcgc agcggatgcc tgccgcataa aaccaccagc ggcgcgttat
    28261 ccagataagc attggcaatg ccggtcaggc cgttggcaaa tcccggtccc gcagtgagaa
    28321 aacatacgcc gggctttccg gtgtaaaccg accaggcatg cgccatcatg gcggcggcct
    28381 gttcatggcg aacatcgatg gtgcgaatct tgtactccgt gaaactgtcc agcaccgctt
    28441 cgatatgtcc gccggaaatt ccgaatacga tatcgatatt ttccacttcc ttcagatatt
    28501 ttcctaccag atgtccgccc attatttttg ccattgtttt tctcctttaa taatcagtta
    28561 ctgacgataa ttaaaaaatt taatctcatt tttttggaca aatcatattt ctcctccctt
    28621 gagggaaggc gttagaggag taaaattcac atcaccagtt tcacaccgtc attgaaccac
    28681 tgcgcgaaaa aagccatgcc gtcgagcttc atcttgcccg cggcggcagc tttgaactgt
    28741 tcttcattac tcttggatgc catcaccctg aaccccgtgg ccgcatccga ccagacaatg
    28801 gccgcatcat acggatgatc ggcgccgcct ttggaactta ctgtcccccg gtcaaagata
    28861 aataatcggc cccgtttacc gtccgccgtc ttgatcatga ttctgacctg atgaatagag
    28921 ccgacgtaac tgcgatagac cggtttcatt ttcgccgccc atttgaggat ttgatacaaa
    28981 atgaacaaca gcgctgaaaa tctcatggtg ctcccctccc ccgatgaatg tttttattcg
    29041 accctgatgt gtgacgtgtt tatggtagtt taaccaaaat taataaactt gacaatgacc
    29101 gggaaagaca attatattgt catttacggc caggataaaa accatgaatc aacaatccag
    29161 agcagattcc atccatctga tatcgagccc gacattgcca tatttccgct ccgtgaacgg
    29221 gattgcgatt ctggcccgtt atgtggaaaa gtacggtatc catgtcaata ctctgctgac
    29281 cggcagcgga cttcatcccc gcgatctgga cgatcctgaa atgtttgtca cgcccgagca
    29341 ggaactcgcg gtgatgcgta aaattctttt tctggtgccg gacccgaaga tcggccttat
    29401 tatcggacaa cagtaccatg tcggggtaca gggcaaactg ggcgccgcag caatcttcag
    29461 cgacaccgga ctcgaagcca tcaccatgtt tttcaagtat atcgccctta ccctcaccta
    29521 ttttcaatat gagctgcatg tcaaagacaa actggctttt gtaaacgtga gagaactgat
    29581 cgacctgaaa gacctgcgag tgtttgtctg tgagcgagaa tttgtttcca tctaccggat
    29641 tgtcagcgat atcctcaagg cccccattcc ggtcaatgaa atccggtttg cttatccgaa
    29701 accatcctat gcctctttct atgagggtgt ctttcattgt ccggtcaaat ttaatgcgcc
    29761 tgaacacagg attattttcg acagcacatt gctttccatg ccgctgccca tgtccaatcc
    29821 catagccagg aaaacgtacg agcaggaatg caagcaaatg tgtcaacggc tgaaggtaaa
    29881 agagacgatc accgatcaaa tccggcagga aattcttttc aaggaggaaa gtgtcccgag
    29941 tttcacgcaa ctttcccggc acctgaatct ttcacccaga accctcagac gtcgcctgac
    30001 ggatgaaaag acatcctata aatcgctggt tgcggatatc cggaaaaaga aggccctcga
    30061 ttggctcaag acgtcggact gtccccttga gacgattgcc acaagactgg gttacagcga
    30121 tatatcaaac ttctaccgcg ccttcaaacg ctggaccgga cgcaatccag gcttttaccg
    30181 gaaagagcat ggcaggatat gatgatcatg acatgccgcc ttcatctgaa aggtcaaatg
    30241 taaaaaaagt ataccgtgaa agatatgatc atttttgaat accgacttgg acaaacaaaa
    30301 gaggttcttc aaaaacttct gcgctctatt caccgtcttc gtatttatgc agtcttttac
    30361 gcagtgtttt tcgtgtaata cccagacgcc gcgcggcttc acttttgttg ccgcgacagc
    30421 tttgcagcgc ctccagtata ctttgcttct ctatttcctc cagagaacga ttcccggaag
    30481 ctgccggcat tttctgcccc gccgacaata cctcgcgaga gagggcctga tccgccatgg
    30541 taaaaatcag gtcttcttca ccgatggttt ccgtcctgca aagaacaacc gccctctcca
    30601 ccgcgttcat caactcccgc acattgcccg gccaggaata actcgccagc ttctgcatcg
    30661 cctgcggcgt aaagcctttg atgactttgg cattgttttc cgcaaacatt ttcagaaagg
    30721 cgtgcgccat gagaggaata tcctcggacc gctccctgag agggggcacg tccagcgtca
    30781 cgacgttgag acggtagaat aaatcttcgc gaaaacgtcc gtcgcggatt tcctttttca
    30841 agtccttgtt ggaagcggcg atcacgcgca catcgatttt aagaacatcg tgtccgccca
    30901 cgcgcgtcag ttcccgctcc tgtaatacgc gcagcagctt aacctgcatg gcgggcgaca
    30961 tttcgctgac ttcgtcgaga aaaatactgc cgccgtcggc ctgaacgaac ttgccttccc
    31021 ttcgcttatc cgctccggta aacgcgccct tctcatgacc gaacagttcc gattccagaa
    31081 gcgtctcggt aagcgccgcg caattgatct taacaaacgg cgcgttcttg cgggcgctgt
    31141 tgaaatgaat cgcattggcg atgacttcct tgccggttcc ggattcgccg gtaatcagaa
    31201 cggttgcctg tgtggcggcc acctgttcga ccacctccag cagtttgatc atcgacgggc
    31261 tctgtccgat catgtttcgc cggtcgaatt tttcacccag tttctctttc agatattcat
    31321 tttccttctt caagcggctg tgctcggtcg cccgggcaat ggatacctgc aattcgtcga
    31381 agtccagcgg tttggtcaga taatcgtaag cgcctttctt cagggtttgg acggcggttt
    31441 ccaccgacgc gtatgccgtc atgataatga ccgggatgtc cgcattaatc tgtttgatct
    31501 gttcaagcgc ttcaatgccg gatacattcg tcatccggat gtccatcaga atcaaatcaa
    31561 aagagctctt ccggactttt tccaccgcca ccagaccgtc atccgcttcg gtcacattat
    31621 acccccagcc gtttagaagc tttttcagca tgacgcgatg cgcacgatca tcatccgtaa
    31681 tcaaaatatc gagcctggtt ttcattatgt cttctctctt tcccgaaaaa taaatattat
    31741 ttctcaagcc ggttgaggaa atcaggggct ttgctttgcc ggacagttat gatgaaagca
    31801 ttcacgttaa acagtttgtc cgccccatgt cctgcatcgt atttgcgaac tcaatgactg
    31861 atgaaggttt ccagatttca acacccttca cttcttaaaa cgtcgtgtct tacgacacgg
    31921 caaaccgcaa cgtgaccttc gtgcccgcac ccgcctgact ttcgatgttc aaaaaagcgc
    31981 cgtgcgcttc cataatcttt tgcacaaccg ccagccctag gcctgtcccc gccggtttgg
    32041 aggtaaaata aggatcgtag atccggggca gatcattttt ctcgatgccg ctgcccgtat
    32101 cggaaatgag cacttcctgc ccggcctgtg tttgtgagat ccctacggac aagcgcccgc
    32161 cgtccggcat ggccgccagg gcattcagaa atatattcaa aagcacctgc ttgattttat
    32221 ccggatcgat ctcaacagcc ggcagatctt ccggcacacg cgcatccacc tgcacgccgt
    32281 ttttccgcgc ttcaacttca atcaatttga cactttgcat caccaaaccg gatagggcgg
    32341 ttttttctgt cttcagcgtc aggggtcgcg caaattcgat cagttgaccg atgacgcggt
    32401 tgagtcgttc agtttcggca atcataatat ccgccgtttc ctcatcatcg ggatttccgg
    32461 caaaccgctg tttgaaataa accgcgaatc ccttgatgga actgagcgga tttctgattt
    32521 catgcgccac acccgcagcc agcgatccga tggcgttcag atgtcggctg tgcgccactt
    32581 ccttctccag ccccctgacg gctgtcacat tacgcgccag cagaattttc ccctccggaa
    32641 catcccgatc ggcaaaagcg gccaccacca cttcccagat ctgttctcct tttgtttctg
    32701 aagaaagcac gacatcttct tcgatcagac cggacgacat cgtcaccttt gtcagcaacc
    32761 ggaccagcgg gcccggaagt atccggccgg catcctgtct ctcaacattc gacggatcac
    32821 cggcgaacaa atcacgggcg tgttcattgg acgcgataat ccggttgtct ttgtccacgg
    32881 ccagaagccc gatgggtaaa tttctcacca gcgcttctgc gaaaaccctt acgcgcgaca
    32941 gtgacgcacg tgtcaaccgg tatgtctgga ccagaaacaa tgacacaatc gcaagcgatc
    33001 cgatcagaat cagaataaac gccctgatga tggtccggta aatatcttcc tgcgacgcct
    33061 tttcgatctt atccatgttg aatcccacaa acacaatcag cgaacgatga tgtaacgccg
    33121 cgtctgtttg ccgccacggg aacaatcccc ggtagatttc aaatgttccc gcgccgccgg
    33181 gattggttac ctgacgccag tgcatgtcct tgcttcgggc aatcttttcc gtatccagat
    33241 ctatgccata ctgctgatcc agcatggagg gatcgctgtc cgccagaatc tttcccttgc
    33301 tgtccgtgac gatcatgtaa tcgatatccg gctgctccgc cgttgccatc agcagtttct
    33361 ggaaataaaa acgatcgttt ttgccatcca cacgatcttg caatcccgcc tcaaaagatt
    33421 gaatcagcgt tgcgcccttt tcgataaaaa gcgcctgagc ctgtgtctcg ctctgctgaa
    33481 actgcatgaa catcatcatg cccagaatca ccatcaaaac ggtaatcgcg ctgacagccg
    33541 cccaaatgga aatgtcccag cgtatttttt tctttttctc ctgcatcatt accttgtccc
    33601 gccatatttg ggcacgatgg acccatctca atatccgagc cggatattta ttatccactc
    33661 attattcata aaaggcaaac agaaaacatt ccgtaataaa ccttcgttaa tcattacaaa
    33721 tccttatcat cacggcaagg atttgccctc atatccggat catcaaaaaa tatgcaaatc
    33781 attcctcatc gtgcggacct tgcctgcata atatctgtcg cgtgagacga ttgtatcttt
    33841 ttaaggatgt acctgtttat gaagaatcat tactacgggc aggggattta tcggacggca
    33901 aaacaattga tcagaaaaat cattaatcgg cgcttactta aaaaccctta ctttgataaa
    33961 attcatcact tattgacatg ccttttttat gagggcattg aagaacggat tatcgggtat
    34021 cttaacccac tttggattat caatgacatc ggagctgaag agcttaccgt ccttgtcaaa
    34081 ataaaaaatg gagacatgtc tgattttctt ttctctgcaa tctatttcat tcaaggattg
    34141 aacattcgat atttcttcat attccacggt ggaaagtttt tttttggctc tgtcccggat
    34201 aaacgcctct ctgcctttat cggaaaaaac atctttaatg acatatttca cggcgccgtc
    34261 tttagaaacg acttttttgt aaaaagcaac attccccaga ttgcccgtgt aaaattcaat
    34321 ccattcggaa gcgccgaata caggacttgc cggatgacag aagatcatta atgtgagaag
    34381 cataacccaa cgcattgttt tgatcacgat gcacctctga aaaaagaaaa tttctttggt
    34441 actatactaa cacaataacg aattaaaaaa aacagacata cacactaccg gaaaaaaaca
    34501 tacggccgcg gatccgttcg cagcggatgg gccgccaagc ggacgccgcg tcaatatttt
    34561 gatacatcca ccgttcggat cgaacaaaac gatatgttct ttaacggctt acccgctgga
    34621 tagaagatac gagcgaagat tgattttgga atttctcaga ttcaattttt ttcttagatt
    34681 gtttcgatgg gtcgcaatcg tcttggttga tgcgttgagc atctcagcaa tttcttttgt
    34741 tttcttcccc tttttgatca ggctgacaat ctgcatttcc tgaggagtaa gatgattgaa
    34801 ggaggattgg agatttttca taaacggaga caaaacctcg ttcagattgc tttccagaat
    34861 gtccagataa tttttactgc gtccgtcaag gttcaaacgt ttcagttttc tcaaataagg
    34921 cattacgaga tcacgcatgt tggtctgcaa cttttcttcg atttcctgtt tgtcttcatc
    34981 ccgttgattc atcaacacac gcagcgtgat attagcctct tccagatctt cagcaaaact
    35041 tttaagctca tcctctgcct gcttgcgctc tgtgatgtca tgcgcaacaa catcggcagc
    35101 aaccacgcgg cctttcacat cgcaaacatt tctaatttgc agttgaatat aaaacgactc
    35161 gcctttttta ttcaatattt caaattccac cgagtccgga acttccaaat tttgagacaa
    35221 tctttccacg cgttccaaaa atcttttttt gctttcttcc gtaagaatgt caaatgctgt
    35281 aagagacgtt acctcttcct gactacagcc gacatattta caaaaaacat cattcgcttt
    35341 caggaatctt ccggtttgat aatcgatctg atatat
//
LOCUS       DAOOLB010000018        33557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_1080_length_33557_cov_38.145126, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000018 DAOOLB010000000
VERSION     DAOOLB010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 33557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33557
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1080_length_33557_cov_38.145126"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="PLF77_04015"
     CDS             complement(<1..1663)
                     /locus_tag="PLF77_04015"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08055.1"
                     /translation="MQEPISTMSTSHMAASQAGQLVSLVDCREHKSTETTISDNLDYL
                     QAVEREGQLILAQALLRQRGADWQSHPNYVQAISIAGLTPAECRPEKLSELIALTEQQ
                     NRRGAERAEQKGIEIYFIKFCCENKIEGFNRKLLMLLLMLATNERFAEMFDLCNFGEK
                     ARNMSEVKIGTILNIISSDYRQQLVNRKYFSMESTLIQQDILHIECDNSSIFERYVSL
                     NDRYVRYFVGDNNIYKSTSSLIQRDVGLVHLDQVIMPEETKKEIVSRVGKYLACRNNK
                     EVTMLDAFYGYGTGLTLLFHGPSGTGKTMMAQALACHFNRPLYSLKMSDFRNERFTIA
                     DDAIKELFLEASLNSGIVYFDEADDFFVKDSLFSRSLLIEIEKARCVVILSTNKPSDL
                     DPALDRRLSLKVCFSIPDAELRCKMWQALMPGFVTLSDDIDFRELAERYNFTGGLIKN
                     SIFMALISSMTNNKKGKSLITMDMIEQACRLQLQQMYDMKDLYQIYTPQCRCSDVQKY
                     IGQKIKISNVAKVYKSLKEKKMGLNILVTSSDIQTGIDVVEALANE"
     gene            complement(1676..2242)
                     /locus_tag="PLF77_04020"
     CDS             complement(1676..2242)
                     /locus_tag="PLF77_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPC08056.1"
                     /translation="MAKEDIKREDKALAQYGIIYLSGDIDTASAETICKEIIEHNIKG
                     DVSQMQMIINSPGGSCTAGFSIIDIMEWSRIPIYTTGLGMIASMGLMVFMTGEKGHRV
                     ITPRTSILSHRYTAVSHGNHSQLIAGRKEEDMMHERGVQHYLTYSNVKDRAELENYLL
                     RDVDTWLTPEEAVKYGLADIVEPTRKHA"
     gene            2509..2727
                     /locus_tag="PLF77_04025"
     CDS             2509..2727
                     /locus_tag="PLF77_04025"
                     /inference="COORDINATES: protein motif:HMM:NF023331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08057.1"
                     /translation="MATAVRISAELVDEAKRFSRIDHRSLAGQIEHWARMGKCAEENP
                     DLSYSLIKEILLGLDELDQGDKTEYRFG"
     gene            2734..3015
                     /locus_tag="PLF77_04030"
     CDS             2734..3015
                     /locus_tag="PLF77_04030"
                     /inference="COORDINATES: protein motif:HMM:NF027117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HPC08058.1"
                     /translation="MKIYQSKLFEKKIKKMSKAEKTALDREVKNIAENPNVGEEKKGD
                     LKGVFVHKFKLKTNLYLLAYRKAGSDLELIMIGPHENYYRDLKGYLKSR"
     gene            complement(3066..4070)
                     /locus_tag="PLF77_04035"
     CDS             complement(3066..4070)
                     /locus_tag="PLF77_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HPC08059.1"
                     /translation="MPEKFDPYKSYGEKLIGLFVRLLFSGESHSLTDLARMLDCSKQT
                     VLRLIDNIRKAYDINIEETKQGNRSYYRIRKATASLQNIPLTEAELQSLQLCKAFTRH
                     LVGKKLYEEATQAILKSKTSLAAGHQVGECHFTAFRPGTIDYTPQHQIIHTLIDAMEK
                     HEVCKLTYQSMNSPRPKTYYIKPLKLFSHHDAIYLHAQKAKEPGKPYRAPQYDPLLAV
                     HRMKAIELTNISFQPDKFDFEKAFNQQFGIIKDEAFRVEAQFTGWAASFVLERIWSPD
                     QEIVKKGKNKILLKFTASSEPELISWLLSFGEQATLIKPDWLVKKLKLAIGGMEACYS
                     "
     gene            complement(4396..4773)
                     /locus_tag="PLF77_04040"
     CDS             complement(4396..4773)
                     /locus_tag="PLF77_04040"
                     /inference="COORDINATES: protein motif:HMM:NF037832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5615 family PIN-like protein"
                     /protein_id="HPC08060.1"
                     /translation="MNTTKPLSFLADESCDFSVIRALRSVGYSVKAIAEISPSLPDEA
                     VLELAVAEKRLLITEDKDFGEWVFAHQHVMAGVLLIRYPSSTRSSMGAAVIDLIGGHA
                     PELDGSFTVLEPGRARIRTKIKD"
     gene            complement(4770..5012)
                     /locus_tag="PLF77_04045"
     CDS             complement(4770..5012)
                     /locus_tag="PLF77_04045"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HPC08061.1"
                     /translation="MNTYDLIETKPEVMLGKPVIKGTRIPVDLIVRKLGEGASFEDLL
                     DGYPNLSREAIQAALIYAADMIRNETTIFLKTGTSN"
     gene            5410..6012
                     /locus_tag="PLF77_04050"
     CDS             5410..6012
                     /locus_tag="PLF77_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08062.1"
                     /translation="MGRYSIIIEDKNIYHALICRVVNIIKDKDLYNGRFTIKQLTSLE
                     IFSLEEEEYLKKIFSPDIDELDIWGEHYFYFIKNAWEYSEYIFYDYRKVIRMMMLLLN
                     KSKIPLCTKVRTDDYHLISKLWHMKILWESAALDADWENRECSVNYAVIELLSHNLKD
                     RLGDSCRFDFRFIKAFMEARTDYFRKKVNDAIMGIESINN"
     gene            6060..6539
                     /locus_tag="PLF77_04055"
     CDS             6060..6539
                     /locus_tag="PLF77_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08063.1"
                     /translation="MKLQELFDSSEYFKEIVNKQIPELNFDIEFVKYKKLFKDSFRES
                     DPPSATHKFSSGVYIFCDNKDNIIYIGKAANGNLAFRIWDHLKTPNDVNQFEKSPWVT
                     NHAYDKQARAIILKGNFTVSAIVVEPKELSSLLEVYLQTIHYIKEKKNLPVLNSQIG"
     gene            7152..8054
                     /locus_tag="PLF77_04060"
     CDS             7152..8054
                     /locus_tag="PLF77_04060"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HPC08064.1"
                     /translation="MIKAIGSETYIEIGIGPHCSDPYECPLTAQCWSFLPERNVCLLY
                     SNNKLPMTLIRDGILELAKIPEDYELNKKHQIQVDCEKSGNPYIDSKKLKEFLDTIQY
                     PAYYMDFETCGSVIPPFDNSSPYQQIPFQFSVHVVQKKGAKPEHFSFLADGTNDPRPE
                     FMARLKDVMGTKGSVIAYNAGFETGILRKCAEVLPQYKNWVESINERMIDLLQPFRAF
                     AYYHPAQDGSCSIKNVLPAMTGKSYDDMEIGDGGMASSEYYRITFTEDNKDKDKVRKY
                     LEEYCGQDTMGMVNIVNKLYSFTK"
     gene            complement(8078..8430)
                     /gene="ssrA"
                     /locus_tag="PLF77_04065"
     tmRNA           complement(8078..8430)
                     /gene="ssrA"
                     /locus_tag="PLF77_04065"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8516..8992)
                     /gene="smpB"
                     /locus_tag="PLF77_04070"
     CDS             complement(8516..8992)
                     /gene="smpB"
                     /locus_tag="PLF77_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPC08065.1"
                     /translation="MAKEKTAQKLIASNKMARVNYEIGDIYEAGLVLTGTEVKSLRAG
                     KANLKDSYAVVKDDEVYIKEMHISPYDFGNRSNHNPLRTRKLLLHKREIKKLYGKSRE
                     KGLSLIPLKLYFKNGKVKVEIGIGKGKKLYDRRQDIKQREERRSLSRDFKSRQIKV"
     gene            complement(9082..9840)
                     /locus_tag="PLF77_04075"
     CDS             complement(9082..9840)
                     /locus_tag="PLF77_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08066.1"
                     /translation="MNQDTKDRNTKLFLHMKKWLEKAVLDYKMIQKGDRLLIGVSGGA
                     DSLALLDLLDSPMLFIPDFSFVAVNIDMGFDPAYKKYDRLEKYFQKNNYPYVMEKTDI
                     GTVAHSDVNKKNPCFLCSRLRRKRIFEIADAEGCNKIAFAHHRDDIIETLLINMFYGR
                     EISTMMPNQSIFGGKLHIIRPLIYLREELVKKYSKERRFPVLKNDCPTGKTSKRIYIK
                     KLLNKLERDNKEIRDNIYKAMSHVKPDYLLPQKK"
     gene            9987..11300
                     /gene="eno"
                     /locus_tag="PLF77_04080"
     CDS             9987..11300
                     /gene="eno"
                     /locus_tag="PLF77_04080"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPC08067.1"
                     /translation="MPEIIHIHAREILDSRGNPTVEAEVTTLSGITARAAVPSGASTG
                     EHEMLELRDGNKKRYNGKGVEKAVDNILHKIGPEIIGMDCRRQRDIDFTMIEMDGTAN
                     KGKLGANAILAVSMACAKAGAEASGLPLYRYLGGVNSHVLPVPQSNILNGGQHADNNV
                     DIQEFMILPVGAPNFKEGIRMNAEVFHALKSVLKGKGYNTSVGDEGGFAPNLKSNEEA
                     LACIMTAIDKANYKAGRDIMIALDCAASSFYEKGKYILAAEAKPRKSSEELVKFYAEL
                     VAKYPIISIEDGLAEDDWSGWKVMTEELGGTIQIVGDDLFVTNVKRLNKGIDLGIANS
                     ILIKLNQIGTLTETLETMSRAKECGYTCVVSHRSGETEDTFMADIAVATNCGQIKSGS
                     LSRTDRLAKYNQLIRIEEELGDAAVYRGKETFYNMKQPAKSGRKR"
     gene            complement(11413..14034)
                     /locus_tag="PLF77_04085"
     CDS             complement(11413..14034)
                     /locus_tag="PLF77_04085"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPC08068.1"
                     /translation="MEENNHPVKSNQGIQYYSGLLYDNQNFLQFRLAKTFLSRVSIAD
                     EYVENIKKLSGEGVVVYALNQRSELNSLILYELLQRNDMPVPVYCHGMNMSLAHPLPQ
                     MLKFFWSSLKRRMGRERKAWQGKLAFIEKTIMEKKSIVIHLGESEFIENRSAESAMST
                     LLDIQKKAGFPVFVVPMLVSYGRRREKEDEGLINILFGQTEHTGALRRLITFIRYSRS
                     AFVIPSEPVNLSEYLKTTKKTATDAMVHELRGELIDRIDKEKTAIVGPALKSREELIG
                     MVLSDESLKAFIEKHAQEEKKDKARIKKDARRYLYEIAADYSETFIAIWKKVLTWLWN
                     DIYDGLSIDVEGMAKIRNISKKMPFVIVPCHRSHIDYLLIHYVFYINNIQLPFIAAGN
                     NLSFFPMGYIFRKSGAFFLRRSFRGNKVYSEAFTKYLAILLKEGLPLEFFIEGGRSRT
                     GKMVMPKYGLLSMVLQAYQEKYCENFAAIPVYIGYDRVIEEKSYLKELSGETKTPENT
                     AEIIKTSKILTKRFGRVYVNIGEPMIMKDYLAQQAKPLEDMSLEERQSLYRKIGYEIV
                     LEINNVSVVTPFALVAAVILSHDRRGISYNDLLYVLNEFYEYLTVKQVKFAETFAHRD
                     KAIIDALNMFVQAGFIEKIEAEKDEVEEMQEVVYSLKEERRLNLEYYKNNILHFFVPL
                     CFVATSIIKNNEDLISLNRIQGDYGFLKKLLWNEFIFDKHKDEAEEVNEVLSYLYDRK
                     IIVSVEQDGQTLIEIKGRGNKRLKPLADLIHNYLESFWIVMRSCLYLKKNPLPKKEWL
                     KKIMALGDRMYRKGEVLRKEALSQANYQNVIIYLEDADLIKSFRDEKIDKKDVLYTLA
                     ENRAEMEVLRRRLFRFL"
     gene            complement(14078..15859)
                     /gene="ptsP"
                     /locus_tag="PLF77_04090"
     CDS             complement(14078..15859)
                     /gene="ptsP"
                     /locus_tag="PLF77_04090"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HPC08069.1"
                     /translation="MSPDQTTKTFVLKGVGVSPGIAIGKCYRYDPLDSQISFYKLKDE
                     SLIPEEISRFKNALKESEQQLLKIQKNLKKANVAEPVYVIDVHILILKDKIFINRTIK
                     YIREMSVNAEWALRMTLDHYKEVFEGVEDDYISSRVSDVQYVVQRILRNLSGGKSEIV
                     WETENRGVIIVSHDISPADTAQMKLDKILGFATDSGGRTSHTAIVARSLELPAVVGLD
                     DITRFVHEDDDLIVDGTAGMVVINPYPDMLKRYEEKKRHYDAANDEYLKYGRQPAVSL
                     DQHRIQIGCNIEFIEEIPSAVAHGAENIGLYRTEFLYIYRENLPTEEDHFTNYRQVVT
                     NKNLAWSTIRTFDLGGDKFPNYQKQSKEMNPQMGMRAIRFCLKEVDIFKIQLRAIWRA
                     SALGNIKILFPMISSVDEIREAKKILEEARRELIDEGMKIPEKIDIGAMIEVPSAVVI
                     ADKIAREVDFFSIGTNDLIQYSLAIDRANERLTYLYEPLHPAVLRQIKKVVDAAHKAK
                     IEVSMCGEMAGDPLCVLVLLGMELDELSMNHLAIPRIKKIIRESTLKESKSMLKRALS
                     FNTASEVRSYVQKYMARRFPEDFKTED"
     gene            complement(15867..16136)
                     /locus_tag="PLF77_04095"
     CDS             complement(15867..16136)
                     /locus_tag="PLF77_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HPC08070.1"
                     /translation="MTETKTLRLKNKLGMHARAAASFVKVAQQFQSEIYIEHNGQTVD
                     GTSILDILTVACPYGGELTIKADGADAAAAIEELVKLIENKFGEE"
     gene            complement(16133..16888)
                     /locus_tag="PLF77_04100"
     CDS             complement(16133..16888)
                     /locus_tag="PLF77_04100"
                     /inference="COORDINATES: protein motif:HMM:NF015570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HPC08071.1"
                     /translation="MKKTTLTSIFFRSFFIHATLNFRRMQNMGFGMALIPMIRELRLQ
                     KKDAVKILTAHLQMFNTHPYLSAPIIGSIVRLEEDHVAKGENSDTASIRQSLMASYAA
                     IGDIFFWGAFRPFAAIVAVLLVHIGLAMAPVIFLLIYTPAHLWVRLKGFIEGYRKGKT
                     GFEFIRALNLPAAAVRIRWITLMVLTGLMIWLARDGGYWPFGETYGVVAKLIALATVM
                     LCYILIKKGISQVYIVYGAVLAFVIISWTGWIS"
     gene            17086..18453
                     /locus_tag="PLF77_04105"
     CDS             17086..18453
                     /locus_tag="PLF77_04105"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08072.1"
                     /translation="MSRKILLINPWIHDFAAYDFWIKPLGLLYLGSLLRKNNHRIDLI
                     DCLDPFHPQMAASGLKKPRRYASGDGKFYRQIIDKPAPLKWVDRSYCRYGILPDVFAA
                     ELKMHRDADMILMTSMMTYWYPGVFEAIRIIKDTLPQIPVVLGGKYATLCYDHASNFS
                     GADYVIAGAGEKQILDLLEKVFSEKTLFVPDENNLDSCPYPAFDLISKTGQLPVITSR
                     GCPYRCSYCSSHILTPGFRRRDPVKVADEIEYWHRKTGVVNFSFYDDALLVNPEAMIV
                     PLLDELQRRNLSCRFHCPNGLHLREITKELAERLYHAGFKTIRFGFETSDLDRQRQTG
                     GKVKNEELRQAVEHLKNAGYRTEDLGIYLLCGTPGQTAREVINSIEFVQECGAKPLLA
                     EYSPIPGTAMWQEAVACSPFDILHEPLYHNNSLLPCGNDDFSFARYTELKKKLKNGSR
                     TGAPS"
     gene            18450..18686
                     /locus_tag="PLF77_04110"
     CDS             18450..18686
                     /locus_tag="PLF77_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08073.1"
                     /translation="MKVLIWIKNVLILLVSLFFLLIGINTLMGSYRINNPMEFLMYFF
                     SASLLILVCIVGILYFIFRLFPQKQEGLNKDESV"
     gene            18673..20178
                     /locus_tag="PLF77_04115"
     CDS             18673..20178
                     /locus_tag="PLF77_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPC08074.1"
                     /translation="MKACKILKAALLPAFIFIFIAAGSGHASDLKEKVLKQKLPNGIT
                     VLMLKRHLSPTVSLYIRYRVGAVDETKGESGAAHFLEHIMFKGTTSIGTNDYQAEKAL
                     MEEIKNAGRRLDAEKRKGQTADPETLANLSAVLKKLQDEHKKYFLPNEIDRLYTENGG
                     LDMNASTGQDVTSYHVSLPANKIELWARIESDRLLHSVFREFYTERDVVMEERRQRVE
                     SDPEGKLYEKFMSAAYTLHPYGRPVLGWTEDLMNLSPDELEKILKKYKDPRNIVIAVV
                     GDIKPEPTLKLIEKYFGGIPSAAEKTESYIPAEPPQTEERRVNVAFDANPSLIIGFRK
                     PNAPAREDYVFDVIETILTRGRTSRLYTTLVIDRQIAQSVSAVNGLPGSRFPNLFALF
                     AQPRHPHSNIELENDILKEIEKLKTQPVSDEELMKAKNHIKMSYLQSLDANADIASIL
                     SYYEVLLGDYRYFADYLDNIDKVTAADIRQIAARYLNSNNRTTAFLNKEGG"
     gene            20185..21627
                     /locus_tag="PLF77_04120"
     CDS             20185..21627
                     /locus_tag="PLF77_04120"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPC08075.1"
                     /translation="MDKMISKIYPVFLVGVFLFLMMATVRPSSANQADAARPSPDQLQ
                     YEPLFFELPKAHRVVLENGIILYILEDHDLPLVTINAFVKTGSMHDPAGKEGTAELTA
                     QVMRTGGTTRLSSREIDRQFDLIAASASIAMAMESAHVGFSVLTTHLDKGLDLLSQIL
                     IHPVFEQGKLELARQLQLEEIRRIKDDPQKLAMREFSRLIYHQDPRGRFPSSASLTEI
                     KRDDLMAFHSRFFLPNNIMFAVSGDITKEKAVEKIKQYFGGWNTKNSATDFPAPVQQP
                     QTGMYFIEKDIPQSTIISGQFATGKNHPDFHAFTVLDFIVGSGGFPSRIMSTVRNNEG
                     LAYSAGSFYRARPDYGIFGSYAFTKTSSTMKALSLINTVLENIKAVPVAGQELTWAQK
                     SINDGFLFSFATPEQIVRQQMTIEYEKLPPDFLTSYRDKINRVTVQDLHHAAVKYLDQ
                     TKNTVLILGDGKNPDHPLPPGGYTVIAPID"
     gene            21649..22893
                     /locus_tag="PLF77_04125"
     CDS             21649..22893
                     /locus_tag="PLF77_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HPC08076.1"
                     /translation="MIDEKLFQKISQRIDAYRDGMIELQTALCAHPAVGPENGGDGEL
                     VKANFLKDRLLQMGFKNFQHYDAPDNRVSSGIRPNFVTTIEGKNKNRFVWIITHMDIV
                     PPGELRLWSSDPYKAYVKDGRIYGRGVEDNQQDLVASIFAAKALMDEGVVPENSIKLA
                     FVSDEETSSSKGLFYMIDSTDRLFQDNDWIVVPDSGNAEGSLIEVAEKSMLWICFKTK
                     GRQCHGSTPHLGNNAFVAGSHLVTSLIKLRKMFPDTDPLFDPPQSTFEPTKKEANVGN
                     INTIPGEDVFCMDCRVLPEYDLKDVMNAIQKICRKIEKKFNVRIETSMVQYVQSAKPT
                     PSDAPVVLALQDAIDAVYHVKAFTGGVGAGTVASYVRKKNYPVAVWSKTNQFAHQPDE
                     NCPVENMIGNARVFAYLFLRKS"
     gene            23061..24059
                     /locus_tag="PLF77_04130"
     CDS             23061..24059
                     /locus_tag="PLF77_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPC08077.1"
                     /translation="MIKALKNEDAEKIVFADQHLLKNLCGEHDKNLKFIEKLEPVKVK
                     AWGNSVTISGRNGNVHKIKTMLQQMYALMEKGWHIQSADIDYAHRILTEDVTFELASI
                     FLNTVFISSQKRVITPKSIAQRLYIEYMRNADMVFSIGPAGTGKTYLAMAMAVSCLME
                     RKVQRIILARPAVEAGERLGFLPGDLAEKVNPYLRPLYDALYDMLDMERATALIHKGL
                     IEVAPLAFMRGRTLNDSFIILDEAQNTTSEQMKMFLTRLGFGSKAVITGDITQVDLPT
                     EKVSGLIEAENILKKSAGIRFVHFSEIDVIRHPLVQDIIKAYAHLTKSKNTNHHNV"
     gene            24062..26422
                     /locus_tag="PLF77_04135"
     CDS             24062..26422
                     /locus_tag="PLF77_04135"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPC08078.1"
                     /translation="MKYFESLRIKMTEFFRNYLEKAAPYFGFVRNSLFQRWMIAVVLC
                     LILALIMAPELNFSKPQFRIGMIAPKNIKADFAFLVEDPQAAEEKKIQDAENINPIYN
                     YNSQIADNIQKKIVKAFSLAAERYNNVPEKQQQPDKIMDLQKEKKRLENNLGAYLTPE
                     EFYILNEYRFSEELQNKFSQLIISFYDNRFIASDAFGKSEKQKGVVIRNLRTQISEDI
                     RDTSALLNMNEIDDPLLKTINMIFRNDEKPLKDAAFSITKKIIEPNLAFNKGATEKKK
                     MAIIGDAAATFFQVQKNEMIVREGEKIGYLELAKLNAYFKDDQDNRFTRVAIVLGFFF
                     TAMFLSIVLYLWRTRNWVKTSDRSNLDLLVFGIIALLQILFIKIGIFMSIAINRSFSF
                     IPVDACYYAIPFAVGAMTIAILINRNVALIISALTSFLIGFLFDEKIVFPLFSFLGSI
                     AASYQIVRIRQRSVFLKIGLFLGIINMAAIVCLNLITGNFFNDLPIRLIMGFIGGIVT
                     GILVAGITPLFESLFGFITDIKLLELANLNQPLFQQMIIEAPGTYHHSIIVSSLAESA
                     AEAISANALLTKVSAYYHDIGKLKKPQYFIENQPNSDNRHDKLSPKMSSLIIMSHVKD
                     GCELAAKAKLGRQIINIIRQHHGTSIISFFYDKAKKDKNESLRSLSENDFRYPGPKPQ
                     TKEAGLIMLADVIEASSRTLTNPTPARIKSLVRERIEGIYMDGQLDECELTLSNLNTI
                     AETFTKILTGIFHHRIDYPEAPQKEGNGKKESNENTNRKQANPAQD"
     gene            26379..26840
                     /gene="ybeY"
                     /locus_tag="PLF77_04140"
     CDS             26379..26840
                     /gene="ybeY"
                     /locus_tag="PLF77_04140"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HPC08079.1"
                     /translation="MKIQIENRQTLHKIERRKIRSAVSKIFKILECTDRHISILFTDD
                     EQIREINRCYLGRDKATNVISFSMREGEYGHINPQILGDVVISVETARRDAIRAKLML
                     EEEIDFLLIHGILHLLGYNHENTSRKEAKIMYDKERDVFRQMNSRKNMPAQ"
     gene            26867..27619
                     /locus_tag="PLF77_04145"
     CDS             26867..27619
                     /locus_tag="PLF77_04145"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPC08080.1"
                     /translation="MQLSLENKVALVTGGSRGIGRAIALGLAKSGADVVIASRKIGDL
                     ENVAGEIKSLGRKCLPVATHIGRLEEIKNLVDKAAAEFGRIDILVNNAATNPTMVPAM
                     DIDDRAWDSIMNLNLKGLFFLSQAVARIMKEKGGGRIINVASVAGLSPDILPVYSISK
                     AGVIMATKVMAQQWAIYNIRANAIAPSLTKTKFSEPLWTNQDILNIAMSRTPLGRPAE
                     PDDMVGAVIFLASDASDYITGQVLAIDGGMTI"
     gene            27745..28374
                     /locus_tag="PLF77_04150"
     CDS             27745..28374
                     /locus_tag="PLF77_04150"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HPC08081.1"
                     /translation="MQNNIYRLFKLHITLFSKIVIAALLFFFAAAFTDIARADIYKYE
                     DNEGVLHLTNVPSDTGAKYVMVMREKRILFQPQLDVNKYDHIINKTADKFKLDSALIK
                     AIIKAESNFNHRAVSPVGAQGLMQLMPKTASQLNVDDSFHPEKNIEGGARYLRYLLNL
                     YKGNLSLALAAYNAGEGAVAKYNYNIPPYRETQNYVRRVLSFYRSFSGN"
     gene            complement(28376..28972)
                     /locus_tag="PLF77_04155"
     CDS             complement(28376..28972)
                     /locus_tag="PLF77_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPC08082.1"
                     /translation="MLSDWIRKNNIVFISESRLKGLIVLSLMVAVIPFVSLMITSWTP
                     YRIPVYADTCDHCCVIEIVGDDQRAGIYHVPSGMSVNRLLKTAGVEKQFEKDFTVKTG
                     MKLVIRSASDRPGITVAEIQNTAKISVGLPIDVNKAGEEDLILIKGIGPVTAKRILEL
                     RTRLNGISDIRQLMEIKGIKEKRMKHIEKYLYVEKKNV"
     gene            complement(28944..30164)
                     /locus_tag="PLF77_04160"
     CDS             complement(28944..30164)
                     /locus_tag="PLF77_04160"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPC08083.1"
                     /translation="MALIVQKFGGTSVANIEKIKNVAEKVIKVKQAGHQVVVVLSAMA
                     GETDRLINLAHSATDLPDEREYDSLISTGEQVTVTLLAIVLNAMGCKARSFLGFQVKI
                     LTDQAYKKARISLIDTENIKKELKKGTAIVIAGFQGVDKDNNITTLGRGGSDTSAVAL
                     AAALRADQCDIYTDVDGVYTTDPNVCNKARRLEKISYDEMLEMAMMGAKVLQPRSVEM
                     AKKYNVPVYVKSTFSDKGGTLVTREDKDMEREIISGVTYDRDQVKITVVHVPDQPGVA
                     ASLFSPLSDKNIFVDMIIQNASSEGYTDLTFTVSKKDLKEAQKIVESTAKKVGAKKIE
                     VDDQVAKISIIGVGMASHSGVAAKMFKTLAAEGVNIMMISTSEIKISCIIQRKYMELA
                     VSVLHDAFGLDKKK"
     gene            complement(30167..30655)
                     /gene="tsaE"
                     /locus_tag="PLF77_04165"
     CDS             complement(30167..30655)
                     /gene="tsaE"
                     /locus_tag="PLF77_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPC08084.1"
                     /translation="MSQDDKTVEIISVSTENTMEIGRSIGEGLQDGDVVALSGELGSG
                     KTCFTGGLARGLGVDEKYRITSPTFTLINEYPARHRLYHFDVYRISSYSELDDLGCEE
                     YLGTEGVVVIEWAEKIKKILPEKTVYIDFEYVDENRRKMIIRASADRLNELIKDIKTE
                     GV"
     gene            complement(30675..31058)
                     /gene="acpS"
                     /locus_tag="PLF77_04170"
     CDS             complement(30675..31058)
                     /gene="acpS"
                     /locus_tag="PLF77_04170"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HPC08085.1"
                     /translation="MVYGIGVDLVENRRMERIIDRWGVKFLNRIFSTEEMAYCGKHAY
                     PVTHYSARFAAKESFLKALGIGLGMGIKLSDISVVNHEGGQPFLNLSGDAQSYIQKRR
                     IHKIHLSLTHTKKYSAAMVLLETID"
     gene            complement(31058..32263)
                     /gene="folP"
                     /locus_tag="PLF77_04175"
     CDS             complement(31058..32263)
                     /gene="folP"
                     /locus_tag="PLF77_04175"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPC08086.1"
                     /translation="MKIIDIQSTQEAAALFRRIGVDAYGIGAMAPKAVNLNILLEKQP
                     CKIANILKQEMLALGGDAAVARGSVACSVETTDVLMMGTLKQLSRLAAKIARQPFGLD
                     SIARDILRILKNAGQKRFILKTSRRKITLGDRTRIMGILNVTPDSFSDGGTFLSAERA
                     VEYGLQMEAEGADILDVGGESTRPGSAAVPVHVELKRVLPVIKALSRKIKIPLSIDTK
                     KARVAREAVEAGAEIINDISALNGDRKMAGTAAETGAALVLMHMRGNPENMQKGNLAY
                     DDLLGDITDYLSASIGKAIRAGVSKDAMVIDPGIGFGKTVADNYRIIRRLADLRTLGM
                     PILVGPSRKSFIGNVTGDEPRQRLEGTAATVSAAIMNGCHIVRVHDVAAMKKVAAVTD
                     AMTHPRKVI"
     gene            complement(32326..>33557)
                     /gene="ftsH"
                     /locus_tag="PLF77_04180"
     CDS             complement(32326..>33557)
                     /gene="ftsH"
                     /locus_tag="PLF77_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPC08087.1"
                     /translation="TLLARAIAGEAEVPFLTISGSDFVEMFVGVGASRVRDLFSQAKK
                     NAPCIIFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNEGVILISATNRP
                     DVLDPALLRPGRFDRQVVVPLPDVRGREKIFEVHARKTPIAGDVDFAVLARGTPGTSG
                     ADIENLINEAVLNAARANKEKVNMSDFEFAKDKILMGSERKTMVISDEEKRNTSYHEI
                     GHTLVARLIPGTDPIHKVTIIPRGRALGLTQQLPVDEKHTYPRRYLENSISILLGGRA
                     AEELVLKDFTTGAGNDIERATNLARKMVCEWGMSDKMGPLSYGKKEEQIFLGREFATH
                     KDYSEETAKNIDAEVMAIVARNYERSKQLLTDHIDLLHKISGELLEKEVLSGAEIDTL
                     IGDALPKAHRSEPQTSV"
BASE COUNT         8726 a         8304 c         7672 g         8855 t
ORIGIN      
        1 attcattggc cagggcttcc acgacatcaa taccggtctg tatatcgctg gatgtcacaa
       61 ggatattcag ccccattttc ttctctttaa gacttttata aactttggca acattggata
      121 tcttgatttt ttgaccgata tacttttgaa catcactgca tcggcattgc ggcgtataga
      181 tctgatatag gtctttcatg tcgtacatct gctgcagttg caatcggcaa gcctgctcga
      241 tcatgtccat tgtgatcagc gatttgccct ttttgttgtt cgtcatcgat gaaattagcg
      301 ccatgaatat actgtttttg ataagaccgc ccgtaaagtt gtagcgttcc gccagttccc
      361 tgaaatcaat atcgtcagat aatgttacaa acccgggcat caatgcctgc cacatcttgc
      421 agcgcagttc tgcatcgggg atgctgaagc agaccttcag cgataaacgg cgatccaaag
      481 ccggatcaag gtctgaaggt ttgtttgttg ataaaatgac cacacagcgg gctttttcta
      541 tttcgattaa aagcgatcgt gaaaacaggc tgtctttcac gaagaaatca tcggcttcat
      601 caaaataaac aatcccggaa ttcagggacg cttccaggaa cagctcttta atagcatcat
      661 cggcgatggt aaaacgctcg ttgcggaaat cactcatctt gagcgaatac agcggcctgt
      721 tgaaatgaca ggccagggcc tgggccatca tggtttttcc cgttccggaa ggcccgtgaa
      781 aaagcagtgt caaccctgtg ccgtagccgt aaaaagcatc cagcattgtt acctctttgt
      841 tgtttcgaca ggcaaggtat ttaccgacgc gcgacacgat ctcctttttt gtttcctccg
      901 gcataatgac ctgatccaga tgaaccagac cgacatcccg ctgaattaaa ctggatgtgc
      961 tcttataaat gttattatcg ccgacaaagt aacggacata ccggtcattc aggctgacat
     1021 atctttcaaa gatgctggag ttatcgcact cgatgtgcag aatgtcctgt tggataagtg
     1081 tggattccat gctgaaatac ttgcggttga ccagttgttg gcgatagtcg ctgctgataa
     1141 tattcaggat ggttccgatc ttgacctcgc tcatgtttcg cgctttttcc ccgaaattac
     1201 aaagatcaaa catctcggca aaccgctcat tcgtcgccag catgagcaag agcatcagta
     1261 atttcctgtt gaatccttct attttatttt cacagcagaa ttttataaaa taaatctcga
     1321 tccctttctg ctccgccctc tcagcacccc gccggttttg ctgctcggta agggcgatca
     1381 gctctgagag tttttccggc ctgcattccg caggtgttag ccctgcaatg gaaatcgcct
     1441 gaacataatt gggatgggac tgccaatcag cgccccgctg ccttaacagg gcctgcgcca
     1501 gaatcaactg cccttccctc tccacagcct gcagataatc caggttgtct gagattgttg
     1561 tttcagttga tttatgttca cggcaatcaa caagggaaac cagttgcccc gcttgtgatg
     1621 cagccatatg gctcgtactc atggttgata taggctcttg catgatggtt cctccttagg
     1681 cgtgttttcg tgtcggctca acgatatcgg caagtccata tttcacggct tcttcaggtg
     1741 tcagccaggt gtcgacatcc cgcaaaaggt aattttccag ttcggcgcgg tctttcacat
     1801 tggaatatgt caaataatgc tggacgcccc gttcgtgcat catgtcttct tctttcctgc
     1861 cggcgatgag ctggctatga ttgccgtgac taactgccgt gtagcggtgc gagagaatcg
     1921 aggtccgggg ggtaataact ctgtgtcctt tttcaccggt catgaaaacc atcaacccca
     1981 tcgaggcaat catgccgagg ccggtggtgt aaatcggaat cctggaccac tccatgatat
     2041 caatgatgga aaatcccgcg gtacaggatc cgcccggaga attaataatc atctgcatct
     2101 ggctgacatc gcccttgatg ttatgttcga tgatttcctt gcatatggtc tcggcactcg
     2161 ccgtgtcaat atcgccggat aaatagatga tgccgtattg cgctaatgcc ttgtcctccc
     2221 gcttaatgtc ctctttggcc atgataattt tctccttttt gttgtctgat ttgggaatag
     2281 cttaccggag gatcgttcca tattacggaa caattgaaat attttttatt gattgaaagg
     2341 gtgcggtgat cttttagtta ttacgaagac atggaaaggt cgctggtcat ggctgtaata
     2401 attttgcatc tagaatgtta cccgcaaaac gatatacaac aagatgttgc ataatgttgc
     2461 tatttgatat atattgcaac tattagaaat ttgtatggag gaaaaactat ggctacagca
     2521 gttagaatat cagcagaact tgttgatgag gcaaaaagat ttagccggat agaccatcgt
     2581 tctttagccg gtcagattga gcattgggcg cgtatgggaa aatgtgcaga ggaaaatccg
     2641 gatttgtcat attccctcat taaagaaatc ctattgggac tggatgagtt ggatcaaggc
     2701 gataaaacgg aataccggtt tggatagatt ctcatgaaaa tctaccaatc aaaattattc
     2761 gaaaaaaaaa ttaagaagat gtctaaagct gaaaaaaccg ctttagatcg agaagtaaaa
     2821 aacattgccg aaaaccctaa tgttggcgaa gaaaaaaagg gcgatctgaa aggtgttttc
     2881 gttcataaat tcaagctcaa aacaaattta taccttttag catacagaaa agcaggttcg
     2941 gatttagaac tcatcatgat tggtccacat gagaactact atcgtgatct gaagggttat
     3001 ttaaaaagca gatgacgaac tttgattttc atccgataaa cttcaggaac aataggcctg
     3061 ttatttcaac tataacaggc ttccattccc cctatagcta acttcagttt tttgaccagc
     3121 caatccggtt tgatgagagt ggcctgttca ccgaaggaaa gcagccagct gatcagttcc
     3181 ggctccgaag aggcggtaaa tttcagaagg attttatttt tgcctttttt cacaatttcc
     3241 tgatccgggc tccagatgcg ctctaaaaca aaagaagccg cccagccggt gaattgtgcc
     3301 tcgaccctga acgcttcatc cttgataatg ccgaactgct gattgaacgc cttctcgaag
     3361 tcaaatttat ccggctgaaa actgatattg gtcaattcta tcgctttcat gcggtgcacc
     3421 gcgagcaatg gatcatattg gggcgcgcgg tacggtttgc ccggctcttt tgccttctgc
     3481 gcgtgcagat aaatcgcgtc gtgatgggaa aagagtttca aaggcttgat gtaatatgtt
     3541 ttcggtcggg gcgaattcat ggattggtag gtcagcttgc agacctcgtg cttttccatc
     3601 gcgtcaatca gtgtgtggat gatctgatgc tgcggcgtat aatcgatagt ccccggacga
     3661 aaagcggtaa aatggcattc ccccacctgg tgacctgctg ccagcgaggt cttgctcttt
     3721 aaaatggcct gcgttgcttc ctcgtaaagc ttcttgccga ccagatgccg ggtaaaggct
     3781 ttgcaaagct ggagggattg cagttccgct tcggtaaggg gaatattctg caaggaagcg
     3841 gtggcttttc ggatccggta atagcttcgg ttgccctgct ttgtttcttc aatattgatg
     3901 tcgtaggctt tgcggatgtt atcaatcaat cgaagaaccg tttgcttgga gcagtccagc
     3961 atccgggcca ggtcggtcaa cgaatggctt tcgcccgaaa aaagcaggcg cacaaacaaa
     4021 ccgatgagct tttcaccgta gcttttatag ggatcaaatt tttcgggcat acgacctcca
     4081 tgtgttcaaa attatagggt cctattggtt aataatataa cttatcgtat ttctgatgta
     4141 aatctaaaaa taataaatca tgccgtatta tggaatgata tcatgattgt tttttgtcag
     4201 gaatgggctt gtatgaagaa ttgcgatctt gaaactatgt gtaaaaaatt aaggctcagt
     4261 agaaaaagca tggaatacat tcgccaaggt agaaagttga aatctaaaag aaaggctctt
     4321 ataaaagttt atcacctgtg aggtgctcag cagttataag gtctttctca gtttatatgt
     4381 tgcgacctgt ggcggtcagt ctttaatttt cgtcctgatt cgcgctcttc ccggctctaa
     4441 aacagtaaag ctaccgtcaa gttctggtgc atgtcctcca atcagatcga ttacggctgc
     4501 acccattgag gaacgcgtgc tcgacgggta gcggattagc aatacgcccg ccatcacatg
     4561 ttgatgcgca aatacccact caccaaagtc cttgtcttct gttatcagca gccgtttttc
     4621 cgccaccgcc aattctaata ccgcttcatc tggtagtgat ggggatattt cagcgatggc
     4681 cttgacggaa tagcctactg accgcagtgc gcgaatgacg gaaaaatcgc aactttcgtc
     4741 tgccaaaaag ctcaatggct ttgttgtatt caattggaag tgcctgtctt taaaaagata
     4801 gttgtttcat tgcggatcat atcggcggcg taaatcagcg ccgcctgaat ggcttctctc
     4861 gacagatttg gataaccatc gagtaaatct tcaaaagagg cgccttcacc caattttctt
     4921 acaatgaggt caaccggaat tctggtccct ttgatgacgg gtttccccag catcacttcc
     4981 ggtttggtct ctatcaggtc ataggtattc ataacatccc tcccttattg gttattataa
     5041 taccataaat ttagattcag gatagtattt tctttgttta ttttctttgt ttatcttgta
     5101 tgcaatcgcc gccttcttgt tgaaaagtag cggtatggag ttctttttgt agtatggtac
     5161 gaaaccatgc gatggtaatt tattttatcg tagcttgtca aagaagtatt ttaatgatgc
     5221 aaaaaaatcg ggggaattta ttgatacatt tttaaaccaa taaagcactc tcgatacatt
     5281 tgatcttttc aaaaacgctc ttcgctaaat aatgaactga ataaaataat caattaatcg
     5341 tgccgtatta tggaacgact ttagggaata atattcacaa aataaacatc agataaaaag
     5401 gccttaatca tggggcggta ttcaatcatt attgaagaca aaaacatata ccatgcgttg
     5461 atctgtagag tggtaaacat tatcaaggat aaagacttgt ataatggcag gtttactatc
     5521 aagcaactga catcccttga gatattttcg cttgaggaag aagaatatct taaaaagata
     5581 ttttctcccg atattgatga attggatatc tggggcgaac actattttta ctttataaaa
     5641 aacgcgtggg aatacagtga atatatcttc tatgactaca gaaaagttat acggatgatg
     5701 atgcttttac tcaataaaag caagataccc ctttgcacaa aggtacggac tgatgattat
     5761 catcttatat caaaactatg gcatatgaaa atattgtggg aaagtgcggc attggatgcc
     5821 gattgggaga atcgggaatg ctcggtaaat tatgccgtga tcgaattatt atctcataat
     5881 ctgaaagaca gactcggcga ttcctgcagg tttgatttca gattcatcaa ggcattcatg
     5941 gaagcaagaa ctgattattt tagaaaaaag gtgaacgatg ctataatggg tatagaaagt
     6001 ataaataatt gaaagatttt atatacaaat attgttcaaa ttaaaaaagg agatacgaaa
     6061 tgaaacttca agaattattt gactcatctg aatactttaa agaaatcgta aacaaacaaa
     6121 taccagaact taattttgat atcgaatttg taaagtataa gaaactattc aaagatagct
     6181 tccgagaaag tgatcctcca tcggccacac ataaattcag cagcggcgta tatattttct
     6241 gcgataataa agataacatt atttatatag gtaaggctgc aaatggaaat cttgctttca
     6301 gaatatggga tcatctcaaa acgcctaatg atgttaatca atttgagaag agtccgtggg
     6361 ttacaaacca tgcttatgat aaacaggcaa gagcaattat actaaaaggg aattttacag
     6421 tatcagcaat agttgttgaa cctaaagagt tatcatctct tcttgaagtt tacttacaga
     6481 cgatccacta tattaaagaa aagaaaaatt tgcctgtatt aaatagccaa attggttaag
     6541 cgattttact ttctttttaa ttaattacgc ataacaacat caaaatgaac attcgaatat
     6601 ccaaaacaaa atatctcaac ggtctgcaat gtaaaaaact gctctggtat atttacaatg
     6661 aaccggatgc gattccggca ccggatgaag ccaaacaggc tatctttgat caaggtcatt
     6721 tggttggcga ttttgccaag cgattgtttc ctaacggtgc ggaggttgat catacaggat
     6781 catttgaaag aggtcttaaa tcgacgcagg aattcttatc gcaaagagtt ccgatatttg
     6841 aagcggccct ggcctataag aattgctatg cacgggcgga tatcctggaa cccgtcggca
     6901 aagataaatg ggatttgatt gaagtcaaaa gctcgacaga tataaaggac attaattatc
     6961 ccgacatcgg ctttcagtac tatgtttatt ccggtgccgg gttaaagatc aacaaatgtt
     7021 acttaatgtg cattgataac aagtatgtcc gtaagggtga catagcgccg aaaaaaatat
     7081 ttaaaaaaat cgatatcact gaagaaataa aaaaaacgta cagcaaaatg attgaaggca
     7141 atgtggagga gatgattaaa gccatcggct ctgaaacata cattgaaatc ggcattggcc
     7201 ctcactgcag tgacccttat gaatgtccgt taacagctca atgctggtca ttccttcccg
     7261 aaagaaatgt ctgtcttctt tacagcaaca acaaacttcc catgactttg attcgggatg
     7321 gcattcttga attagcgaaa ataccagaag attatgaact gaataaaaaa catcaaatac
     7381 aggttgattg tgaaaagtcg ggcaatcctt atattgattc gaaaaagcta aaggaatttc
     7441 tagatacaat tcaatatccc gcctattaca tggattttga aacatgcgga tctgttattc
     7501 cgccatttga caattccagt ccatatcagc aaataccgtt tcagttctct gtccacgttg
     7561 tacaaaagaa aggcgctaaa cctgaacact tctcttttct ggcagatggc acgaatgatc
     7621 cgcgccctga atttatggcc aggctcaaag acgtgatggg cacaaaaggt tccgtaatcg
     7681 cctacaatgc gggttttgaa acgggcatcc ttagaaaatg tgcagaagtc cttccacaat
     7741 ataaaaattg ggtggaatct ataaacgaaa ggatgattga cctgctccag cctttcagag
     7801 cctttgccta ttatcacccg gcacaggacg gcagctgttc tatcaagaat gtcctgccgg
     7861 cgatgacagg caaatcctat gacgatatgg aaatcggcga tggcggcatg gccagctcag
     7921 agtattaccg gattacattt accgaagaca ataaagataa ggataaggtc agaaagtatc
     7981 tggaagaata ttgcggtcag gataccatgg gcatggtgaa tatagtcaat aaactataca
     8041 gttttacaaa atgactatta gatgttgtaa taccttttgg tggaggcggc gggagtcgaa
     8101 cccgcgtccg aaaaccttcc gctggagctt ctacgtgcgt agccttcgtt ttagaacctt
     8161 cacgctggat tgctccctaa ggccggatct ttccgcgcta tcctgtaaga gttcgccgcc
     8221 ctcccccagg caagaagttt ggctatcctg tctgaatgac gttccattcc gatcaacagg
     8281 agaaccgggt ggaacgttag ccgactaagc ggctaaagcg taatcgtagt tgtctgcgat
     8341 tatatgtttc ctacctgttt aacgaggcct gtaggaacct cggcacgctt ctacaacttc
     8401 aaatgtcccc gtcgaaaccg tgacgccccc ttattacgta ttttcatgta atataaacac
     8461 ttagaagtgt tttgtcaaat tttaccttaa tccatgtctt atggattctt aatcactaga
     8521 cttttatctg cctggatttg aaatcgcggg atagtgagcg tctttcttcg cgctgcttga
     8581 tatcctgacg gcggtcataa agtttcttgc ccttgccgat gccgatttcc actttgacct
     8641 tgccgttttt gaaataaagc ttgagcggaa taagcgacaa tcccttctcg cgcgatttcc
     8701 cgtagagttt tttgatttcg cgcttgtgca aaagcagttt tcgggtccgg agcggattgt
     8761 gattggagcg gttgccgaaa tcgtaggggc tgatgtgcat ttccttgatg tagacttcat
     8821 cgtctttgac aacggcatag ctgtccttga gattggcttt gccggctcgt aacgatttga
     8881 cttccgtgcc ggttaaaaca agacccgctt cgtagatatc gccgatttca taattcaccc
     8941 gcgccatttt attggacgcg atcagttttt gtgctgtttt ctcttttgcc ataatgcaat
     9001 tttacatgaa atacgatgta ttgctatagt tttttcttca gactgtctac catacaacat
     9061 tcactattca ccattctctt ttcacttttt ttgcggcagc aaatagtccg gtttgacgtg
     9121 gctcatggcc ttgtaaatat tatcgcggat ttccttgttg tcccgttcca gtttattcag
     9181 aagttttttg atgtagattc gttttgaggt tttgccggtc gggcagtcat ttttcaaaac
     9241 gggaaagcgt ctttctttgc tgtatttttt aaccagttcc tctctcagat aaatcagcgg
     9301 gcggatgatg tgcagtttgc cgccgaagat gctctgattg ggcatcatgg tgctgatttc
     9361 acgcccgtaa aacatgttga tcagcagggt ttcgataata tcgtcgcggt gatgggcaaa
     9421 ggcgatttta ttgcagccct cggcatcggc tatctcgaat attctttttc gtcttagccg
     9481 ggaacacaaa aagcagggat tttttttatt cacatcggaa tgcgccacag tgccgatatc
     9541 ggtcttttcc atcacatacg gataattatt tttctgaaaa tatttttcca gtctgtcgta
     9601 ttttttatag gcggggtcga atcccatgtc aatgttgacg gccacgaagg aaaaatcggg
     9661 aatgaaaagc atcggagagt cgagcaggtc cagcagcgcc agactgtccg ccccgccgga
     9721 cacgccgatt aacagacgat cgcccttttg gatcattttg taatccagca cggctttttc
     9781 cagccatttt ttcatatgca ggaaaagttt ggtgttcctg tcttttgtat cctgattcat
     9841 cttaaattcc attgactcca tttttctcac ggaccgggtt ctatctatat gagttatgaa
     9901 aagtaatcaa gtttgtttca tcaatctgtt gaaatcgcaa ggcctctctg ttaccattcc
     9961 aggaaagttc atcagggagg tgttttatgc cggagatcat ccatattcat gccagggaga
    10021 tactggattc cagaggcaat ccgaccgtcg aagccgaggt aacgacgcta tcgggaatca
    10081 ccgcgcgggc cgcggtgccg tccggcgcgt ccaccggaga acatgaaatg ctggaattgc
    10141 gcgacggcaa caaaaaacga tacaacggca aaggcgttga aaaagccgtc gataacatcc
    10201 tgcacaaaat cggtccggaa atcattggaa tggattgccg cagacagcgc gacatcgatt
    10261 ttaccatgat cgaaatggac ggcaccgcca acaaggggaa actcggcgcc aacgccattc
    10321 tcgccgtctc catggcctgc gccaaggccg gcgcggaagc atccgggctt ccgttgtatc
    10381 gctatctggg cggcgtcaac tcccatgttc tgcccgttcc gcagtccaac attctcaacg
    10441 gcggacaaca tgccgacaac aacgtggaca tccaggaatt catgatcctg ccggtcggcg
    10501 cgcccaactt caaggaaggc atccgcatga acgcggaagt ctttcatgcg ttgaagtctg
    10561 tcctgaaagg taaaggctat aatacctctg tcggcgacga aggcggcttt gctccgaatc
    10621 tgaaatccaa tgaagaagct ctcgcatgca ttatgaccgc tatcgacaag gccaattata
    10681 aagcaggccg ggatatcatg atcgccctgg actgcgcagc cagttctttc tatgaaaaag
    10741 ggaaatacat tctggcggcg gaagccaagc cccggaagtc gtctgaagag ctggtcaaat
    10801 tttatgccga gttggttgcc aaatacccga tcatttccat tgaggacggc ctggcggaag
    10861 acgactggtc cgggtggaaa gtgatgacgg aagaactggg cggcacaatt cagatcgtcg
    10921 gagacgacct cttcgttacc aacgtcaaac gtctcaacaa aggcattgat ctgggaatcg
    10981 ccaactctat tctcatcaaa ctcaatcaga tcggtacgct gaccgaaacg ctggaaacca
    11041 tgagccgcgc caaggaatgc ggctacacgt gcgtggtttc gcaccgctcc ggcgagacgg
    11101 aagacacctt tatggcggat atcgccgtgg ccaccaactg cggccagatc aaaagcggct
    11161 cgctctcccg cacggaccgg ctggccaaat acaaccagtt gatccgcatc gaagaagaac
    11221 tcggtgatgc ggctgtgtat cgcggcaaag agaccttcta taacatgaaa caaccggcga
    11281 aatccggcag aaaaagataa ccccgcaaca tgcagtgagc cggccttttg aataaggccg
    11341 gctctcccgg tctgaacccg atccgatcaa ccccctcggc gatgcggctt gcgcatcggc
    11401 ggcggtcaat gattataaaa atctgaaaag gcggcggcgc agcacttcca tttccgctct
    11461 gttttcagcc agcgtgtata aaacatcttt tttatcgatt ttctcatccc ggaacgattt
    11521 gatgagatcc gcgtcttcca gatagatgat aacgttttga taattggcct gcgatagcgc
    11581 ttcttttctc agcacctctc ccttgcggta catccggtcg cccagcgcca tgattttctt
    11641 cagccattct tttttgggca gaggattttt tttcagatac agacagcttc tcatcacgat
    11701 ccagaaggat tccagataat tgtgaatcag atcggcgagc ggtttaagtc gtttgttgcc
    11761 ccggcctttg atttcgatca gggtctgtcc gtcctgttcg accgagacaa tgattttccg
    11821 gtcgtataaa taagacagca cttcattgac ttcctccgct tcgtccttgt gtttgtcaaa
    11881 aataaattca ttccataata attttttcag aaacccgtag tcgccctgta tgcgattcag
    11941 cgaaatcagg tcttcattgt ttttgatgat ggaggtggcg acaaaacaca gcgggacaaa
    12001 gaaatgcaga atattgtttt tataatattc cagattgagt cttcgttcct ccttcagcga
    12061 gtagacgact tcctgcattt cctcgacttc atctttttcc gcttcaattt tttcaatgaa
    12121 gcctgcctgc acaaacatgt tcagcgcatc gatgatggcc ttgtcgcgat gggcgaaggt
    12181 ttccgcaaat ttcacctgtt tgacggtgag atattcgtaa aattcattca aaacatacag
    12241 cagatcgttg taggaaatgc cgcggcggtc atgacttaaa ataacggcgg ccaccagagc
    12301 aaaaggcgtg accacggaca cattgttgat ttccagaacg atttcataac cgattttccg
    12361 gtagaggctt tgcctctcct ccagggacat gtcttccagc ggttttgcct gctgcgccag
    12421 atagtctttc atgatcatcg gttcgccgat gttcacatac acgcggccga atcgtttggt
    12481 cagtattttg ctggttttaa tgatctcggc cgtgttttcc ggcgtcttgg tttcgccgga
    12541 cagttccttc aggtatgatt tttcttcgat cacccggtcg taaccgatat acaccggaat
    12601 cgcggcaaag ttttcgcagt atttctcctg ataggcctga agcaccatgg aaagaaggcc
    12661 gtatttgggc atcaccattt tgccggtccg gctgcgcccg ccttcaatga aaaattccag
    12721 cggcagtcct tccttgagaa ggatcgccag atatttggta aaggcttcgg aataaacctt
    12781 gttgccgcga aagcttcgtc tcaaaaagaa tgcgccggat ttccggaaaa tgtaccccat
    12841 cgggaaaaac gacaggttgt ttccggctgc gataaaagga agctgaatgt tattgatata
    12901 gaagacgtaa tgaatcagaa gataatcaat gtgactgcga tggcagggca caatcacaaa
    12961 cggcattttt ttggaaatat ttcttatttt ggccatgcct tccacatcaa tggacaggcc
    13021 gtcgtaaata tcgttccaca gccaagtaag cacttttttc cagatggcga tgaaggtttc
    13081 gctgtagtcg gccgctattt catacagata acggcgtgca tcttttttaa tcctggcctt
    13141 gtcttttttc tcctcctgcg catgcttttc gataaaggct ttcagcgatt catcgcttaa
    13201 aaccatgccg atcagttctt cccgcgattt cagggcggga ccgacaatgg ccgtcttttc
    13261 cttgtcgatg cgatcaatca gttctccgcg cagttcatga accatggcgt ccgtggcggt
    13321 tttcttggtc gttttcagat actccgacaa attcaccggc tccgagggaa tgacaaatgc
    13381 gcttcgggaa tatcggataa acgtgatcag acggcgcagc gccccggtat gctcggtctg
    13441 cccgaaaagg atattgatca gcccctcgtc ttctttttca cggcggcgcc cgtacgatac
    13501 cagcatcggc acgacaaaaa ccggaaaacc ggcttttttc tgaatatcaa gcaaggtgga
    13561 catcgcgctt tccgccgacc ggttttcaat gaactccgat tcgcccagat gaatcacgat
    13621 gctctttttc tccatgattg ttttttcaat gaaggccagt ttgccctgcc aggcttttcg
    13681 ttccctgccc atccggcgtt ttaaagacga ccagaaaaat ttcagcattt gcggcaacgg
    13741 atgagccaac gacatgttca tgccgtggca atagaccgga acgggcatgt cgttgcgctg
    13801 gagcagctca tacagaataa ggctgttgag ttcgctgcgc tggtttaagg cataaacgac
    13861 cacgccctcg ccggaaagtt ttttgatatt ttcaacgtat tcgtcggcaa tggaaacacg
    13921 cgacagaaac gtcttggcca gccggaattg cagaaaattc tgattgtcgt aaagcagacc
    13981 cgaatagtat tgaatgccct gattggattt taccggatga ttattttctt ccatcgtcgt
    14041 tatttctcca cacaactttg acgggcggtc ccgtacatta atcttccgtc ttgaaatctt
    14101 cggggaaacg cctcgccata tatttctgca cataagagcg gacttccgaa gccgtattga
    14161 aggaaagcgc tcgtttcaac atgcttttcg actctttcaa tgtcgattct ctgataattt
    14221 tcttgattct cggaatggcc agatgattca tgctcaattc atcgagttcc ataccgagaa
    14281 ggaccagcac acaaagcgga tcacccgcca tttcgccgca catggacacc tcgatcttcg
    14341 ccttgtgcgc cgcatccacg acttttttaa tctgtctcaa gacagccgga tgcaggggct
    14401 cgtacagata ggtgaggcgt tcgttggcgc ggtcaatcgc cagcgaatac tggatcagat
    14461 cattggtccc gatactgaag aaatcaacct cccgcgcgat tttatcggca atcacaaccg
    14521 ctgacggcac ttcaatcatc gcaccgatgt caattttttc aggaatcttc attccttcat
    14581 cgatcagttc ccggcgggct tcttccagaa tttttttggc ttcccggatt tcgtccacac
    14641 tggaaatcat cgggaagaga attttaatgt tgcccagcgc actggcgcgc cagatggccc
    14701 ggagctgaat cttgaaaatg tcgacttcct tcaggcagaa gcggatcgcc ctcatgccca
    14761 tctggggatt catttcctta ctctgtttct gataattggg aaatttgtcg ccgcccagat
    14821 caaacgtccg gatcgtcgac caggccagat ttttattggt cacgacctgg cggtaattcg
    14881 tgaaatgatc ctcttcggtc ggcaagtttt cgcggtaaat gtagagaaat tccgtgcggt
    14941 acaggccgat attttcagcg ccgtgagcga cggcggaagg aatttcttct ataaattcta
    15001 tgttacatcc gatttgaata cggtgctgat ccaggctgac cgcgggctgc ctgccgtatt
    15061 tgagatattc atcgtttgcg gcgtcgtaat ggcgtttctt ttcctcatac cgcttgagca
    15121 tgtcgggata gggattgatc accaccatgc cggccgtgcc gtcgacgatc aagtcatcat
    15181 cctcatgaac aaagcgcgtg atatcatcca gccccacaac ggccggcagc tccagagagc
    15241 gcgcgacaat ggccgtatgc gaggtgcgcc cgccgctgtc cgtggcaaat cccagaattt
    15301 tgtccagttt catctgcgcc gtatcggccg gtgagatgtc gtgcgatacg ataatgacgc
    15361 cccggttttc cgtttcccag acgatttcac tttttccgcc ggacagattg cgcaaaatcc
    15421 gctggacaac atactgcaca tcgctgaccc ggctgctgat gtaatcgtct tcaacgcctt
    15481 cgaacacctc tttataatga tccagggtca tgcgcagcgc ccattcggca ttgacgctca
    15541 tctcgcgaat atatttgatg gtgcggttga taaagatttt gtctttcaaa atcaggatgt
    15601 gaacgtcgat gacataaacc ggttccgcaa cattggcttt cttaagattt ttctgaatct
    15661 tcagcagttg ctgctcggat tccttcaagg cgtttttaaa acggcttatc tcttccggga
    15721 ttaaagattc gtctttgagt ttataaaaag aaatttgact gtccagcgga tcatagcggt
    15781 agcattttcc aatagcgatc ccgggggaaa cacccactcc cttgaggaca aaagtttttg
    15841 tggtctgatc cggactcata tgatgtttat tcttctccaa atttattttc gatcagtttg
    15901 accagttcct caatcgccgc ggctgcgtcc gcgccgtcgg ctttgatcgt cagctctccg
    15961 ccgtacgggc aggctacggt caggatatcc aggatgctgg tcccgtcaac ggtttgtccg
    16021 ttgtgctcaa tataaatttc cgattgaaac tgctgagcca ctttcacgaa agacgccgcc
    16081 gcccgcgcgt gcatccccag cttgtttttt aatcgtaagg ttttcgtttc cgtcaactaa
    16141 tccatcccgt ccaggaaata atcacgaatg ccaacacagc gccataaacg atataaacct
    16201 gtgaaattcc tttttttatt aaaatataac aaagcattac cgtagccagg gcaatcagtt
    16261 ttgcgaccac cccgtatgtt tccccgaagg gccagtaccc gccgtctcgg gcaagccaga
    16321 tcattaaacc ggtcagaacc atcagcgtta tccagcgtat tctgacggcg gcagcgggta
    16381 aattcagggc gcggataaat tcaaaaccgg ttttcccctt gcggtaccct tcgataaatc
    16441 cttttaatct gacccataaa tgcgccggcg tgtaaatcag aagaaagata accggcgcca
    16501 tggccaaccc gatatgcacg agcagcaccg caacgatcgc ggcaaacggt ctgaaggctc
    16561 cccaaaaaaa gatatcgccg attgccgcat aagaggccat caggctctgt ctgatggacg
    16621 cggtgtccga attttctcct ttcgcaacat ggtcttcctc aagacgcacg atcgaaccga
    16681 tgatcggcgc cgacaggtac ggatgcgtat tgaacatctg caaatgagcg gttaagattt
    16741 ttaccgcatc ctttttctgc aggcgcaact cccggatcat cgggatgagc gccatgccga
    16801 accccatgtt ctgcatccgc cggaaattaa gcgtcgcatg aataaaaaaa gacctaaaaa
    16861 aaatacttgt caacgtcgtt tttttcacgc ttttcaggcc tcaaaataaa taactacata
    16921 ttttcaataa cttttatgcg tggtggaact gattagcata aaagatgaat gaagtcaatt
    16981 gatcacgccc cttcattttg cccctcctgc ggcagtctgc gccgggccga atcataaacg
    17041 tttaggcttt tgtaaatgaa cctgcattgt gataagaacc agccgatgag cagaaaaatc
    17101 cttttgatta atccctggat tcacgatttt gcggcctatg acttctggat caaacctctc
    17161 ggcctgctat acctgggcag tcttttgcgt aaaaacaacc accggattga tttgatcgac
    17221 tgcctcgatc cgtttcatcc gcagatggcg gcatcggggc tgaagaaacc gcggcgttat
    17281 gcttccggag acggtaaatt ttaccggcag atcattgaca aaccggcgcc gctgaaatgg
    17341 gttgatcgca gttactgccg ttacggtatt ctgccggacg ttttcgccgc cgagctgaaa
    17401 atgcaccggg atgccgacat gattctaatg acgtccatga tgacctactg gtaccccggc
    17461 gtttttgaag cgatcagaat catcaaagac accttgccgc agattccggt tgtcctgggc
    17521 ggcaaatacg cgacgctctg ttacgatcac gcgtccaact tttcgggggc ggattacgtc
    17581 atcgcggggg cgggcgaaaa acaaattctc gatttgctgg aaaaggtatt tagcgaaaaa
    17641 acgttgtttg tcccggatga aaacaatctg gattcctgcc cctaccccgc ctttgatctg
    17701 atctccaaaa ccgggcaact gccggtcatc acgtcccggg gatgccctta ccgctgcagt
    17761 tactgttcgt ctcacattct gacgccggga tttaggcgcc gcgatccggt gaaggtcgcc
    17821 gatgaaatcg aatactggca caggaaaacc ggcgtggtga atttcagttt ttacgatgac
    17881 gctctgctgg ttaacccgga ggcaatgatc gtgcccctgc tcgatgaatt acaaagaaga
    17941 aatttatcct gccggttcca ttgtcccaac ggattgcact taagggagat cacaaaagaa
    18001 cttgccgaac ggctgtacca tgccggattc aagaccatcc gtttcggttt tgaaacatcc
    18061 gatttggacc ggcagcggca aaccggcgga aaagttaaaa atgaagaact gcgtcaggcg
    18121 gttgaacatc tgaaaaacgc cggttataga acagaggatc tggggattta cctgctgtgc
    18181 ggaacgcccg gtcagaccgc ccgggaggtc atcaacagta ttgaatttgt acaggaatgc
    18241 ggcgcgaagc ccctgctggc cgaatactcc ccgattcccg gaaccgccat gtggcaagag
    18301 gccgtggcct gttcaccctt tgacattctt cacgaacccc tgtatcataa caattcattg
    18361 cttccctgcg gaaatgatga tttcagtttt gcgcgttata cggaattgaa aaagaaactc
    18421 aaaaacggtt caaggacagg cgccccttca tgaaggtttt gatatggatt aaaaatgtgc
    18481 tgattctgct ggtgtcgctg ttttttctgc tcatcggcat caatacgctg atggggtcgt
    18541 accggatcaa taatccgatg gaatttttga tgtatttctt ttccgcatcg cttctgattc
    18601 tggtttgtat tgtgggaatc ctttatttta tctttcgttt atttccgcag aagcaggaag
    18661 ggttgaacaa agatgaaagc gtgtaaaata ctgaaagcgg ctcttctgcc ggctttcatt
    18721 tttattttta ttgccgcagg ctccggtcac gcctccgatc tgaaagaaaa ggttctgaaa
    18781 caaaagctgc ccaacggcat taccgtgctc atgcttaagc ggcatttaag tcccaccgta
    18841 tccctctaca ttcgctaccg ggtgggcgcg gtggatgaaa caaaaggcga gagcggtgcg
    18901 gcgcatttcc tggaacatat aatgttcaag ggaaccacct ccatcggcac gaacgattac
    18961 caggccgaga aggccctgat ggaagaaatc aaaaatgccg gacggcgtct ggatgccgaa
    19021 aaacggaaag gacaaacggc cgatccggaa accctggcca acctttccgc tgtattaaaa
    19081 aagttgcagg acgaacataa aaaatacttt ttacccaacg aaatcgaccg gctctatacc
    19141 gaaaacggcg ggctcgatat gaatgcgtcc accggtcagg atgtcacctc ctatcacgtc
    19201 agtcttcccg ccaataaaat cgaactctgg gcaagaattg aatcggaccg cctcctccat
    19261 tcggtcttcc gtgaatttta tacggaacgc gacgttgtca tggaagaacg acggcagcgg
    19321 gtggaatccg atccggaggg aaaactttat gaaaaattca tgagcgccgc gtacaccctt
    19381 catccttacg ggaggccggt tctgggctgg actgaagact taatgaattt aagccccgat
    19441 gaactggaaa aaattctgaa aaaatataag gatccccgaa acattgtgat tgcagttgtc
    19501 ggcgatatca aaccggaacc gacactgaaa ttaatcgaga aatatttcgg cggcattccg
    19561 tccgcggccg aaaaaacgga aagttatatt cccgcagaac cgcctcagac cgaagaacgc
    19621 cgggtcaatg ttgccttcga cgcgaatccg tcgctgatca tcggctttcg caaaccgaat
    19681 gctccggcgc gggaggatta cgtcttcgat gtcatagaaa caattttaac cagaggacga
    19741 accagccgcc tgtacacgac ccttgtcatc gatcggcaga ttgcccagag cgtgagtgcc
    19801 gtcaacggtt tgcccggttc aagattcccg aatctatttg ccctgttcgc tcaaccgcgt
    19861 catccgcaca gcaacatcga gctggaaaat gatattctga aggaaattga aaagctgaag
    19921 actcaacccg tttccgacga ggaactgatg aaggcaaaaa atcatatcaa aatgtcctac
    19981 cttcagagcc tcgatgccaa tgccgatatt gcttccattc tttcctatta tgaggttctg
    20041 ctgggggatt acagatattt tgcagattat ctcgacaata ttgacaaagt cactgccgca
    20101 gacattcgac aaatcgcggc aaggtatttg aacagcaata accgcacaac agcttttctc
    20161 aataaagaag gcgggtagcg tctgatggat aaaatgattt caaaaatata tccggttttt
    20221 ctggttggcg tgtttctgtt tttaatgatg gcgaccgtca ggcccagtag cgctaaccag
    20281 gccgacgcgg caaggccttc tcccgaccag cttcaatacg aacccttgtt ttttgaattg
    20341 ccgaaggcgc accgggtcgt tttggaaaac ggcatcatcc tttacattct cgaagatcac
    20401 gacctgcctc tggtgaccat caatgccttt gtgaaaaccg gctcgatgca tgatcctgcc
    20461 ggtaaagaag gcacggccga gctgaccgct caggtcatga gaaccggcgg tacaacccga
    20521 ttaagcagcc gggaaatcga cagacaattt gatctcattg cggcatcggc ttccattgcc
    20581 atggccatgg aatcggcgca tgtcggtttt tccgttctca ccacgcatct tgacaagggg
    20641 ctggatctgt tgtcgcaaat tctgattcat cccgttttcg aacaggggaa actggaactg
    20701 gcccggcaat tacaacttga ggaaatcagg cgcatcaagg atgacccgca aaagctggcc
    20761 atgcgcgaat tctcccgtct gatatatcat caagaccccc gcggacgttt tccgtccagc
    20821 gcatcgctta cggaaatcaa acgcgatgat ctgatggcgt ttcacagccg tttcttcctg
    20881 ccgaacaaca tcatgtttgc cgtcagcggc gacatcacaa aggaaaaagc cgttgagaaa
    20941 atcaaacagt atttcggcgg ctggaatacg aaaaacagtg caacggattt tcccgcgccg
    21001 gtgcaacaac cgcaaaccgg tatgtatttt atcgaaaagg atattcccca gtcgacgatt
    21061 atcagcggcc aattcgccac cggaaaaaac caccccgatt ttcatgcctt cacggtcctg
    21121 gatttcatcg tcggcagcgg cggatttcct tcccggatca tgagcaccgt gcgcaataat
    21181 gaagggctgg cctacagtgc cggaagtttt taccgcgcca ggcctgatta cggaattttc
    21241 ggcagctacg cctttacaaa aacatcgtcg accatgaagg ctctttccct gatcaatacc
    21301 gttctggaaa acattaaagc cgttccggtt gccggtcagg aactgacctg ggcgcaaaaa
    21361 tcaatcaatg acgggttcct cttttcattt gccaccccgg aacaaatcgt ccggcagcaa
    21421 atgaccatcg agtatgaaaa actgcctccg gattttctga cgagctaccg ggataaaatc
    21481 aaccgggtca ccgtgcagga cctccatcat gccgccgtta aatacctgga tcagacaaag
    21541 aatacggtgc tgattctggg agacggcaaa aatccggacc acccccttcc gccgggcggc
    21601 tacaccgtta tcgcccctat agactaaatc accatcaaag gatgaattat gatcgacgaa
    21661 aaattatttc aaaaaatatc gcaacggatc gatgcttatc gtgacgggat gattgaattg
    21721 cagacggcgc tttgcgcgca tcccgccgtc ggaccggaaa acggcggcga cggagaactt
    21781 gtgaaagcca attttctgaa agatcgtctg ctgcagatgg gattcaaaaa cttccagcac
    21841 tacgacgctc cggacaacag agtgtcttcc ggaatccgcc ccaattttgt cacgactatc
    21901 gaggggaaaa acaaaaacag gtttgtctgg atcattaccc atatggatat tgtcccgccg
    21961 ggcgaactcc gcctctggag cagcgatccg tacaaagcct acgtgaaaga cggccggatt
    22021 tacggccgcg gcgtggaaga caatcagcag gatctggtcg cctccatctt tgccgccaaa
    22081 gcgctgatgg atgaaggcgt ggttcccgaa aactcgatca aactggcgtt cgtgtccgac
    22141 gaagaaacct ccagcagtaa ggggttgttt tatatgattg attccacgga cagacttttt
    22201 caggataacg attggattgt cgtgcccgac tccggcaacg ccgaaggatc gctcatcgaa
    22261 gttgcggaaa aaagcatgct ctggatctgt ttcaaaacca aaggcagaca atgccacgga
    22321 tcgacccccc acctgggcaa caacgccttt gttgccggat cgcatctggt caccagcctg
    22381 atcaaactgc gaaaaatgtt tcccgacacc gatcctcttt ttgatccgcc gcaaagcacg
    22441 tttgaaccga caaagaaaga agccaatgtc ggcaatatca acacgattcc cggggaagat
    22501 gtgttctgca tggattgccg ggtattgccg gaatacgatc ttaaggatgt aatgaatgcc
    22561 atacaaaaga tctgccggaa aattgagaaa aaattcaatg tgcggatcga aacgtccatg
    22621 gttcagtatg ttcagtccgc aaaaccgacg ccgtcggatg ctccggtggt cctggcgctg
    22681 caagacgcta ttgacgccgt ctatcatgtg aaagccttta ccggcggtgt gggagccgga
    22741 accgtcgctt cctatgtccg caagaaaaac tatccggttg ccgtctggtc caaaaccaac
    22801 cagtttgctc atcaacccga cgaaaactgc cccgtcgaaa atatgatcgg gaatgccagg
    22861 gtcttcgcct atctgttttt gcggaaatca tgagggcatc cttatgaaaa cagaccacat
    22921 catcatacca ttatgcataa agcacttata attcaagcat ttacggattt atcaataggt
    22981 attgcaatgc cgaaatcaaa atgatagata ccaacgttga atgaaatatt gatcacctat
    23041 tgtagaggtt gataactatt ttgataaaag cattaaaaaa tgaagacgcg gagaaaattg
    23101 tctttgccga tcagcatctg ctgaaaaatc tctgcggcga acatgataaa aatttaaaat
    23161 tcattgaaaa gctggaaccg gtcaaagtca aagcatgggg caacagtgtc accatctccg
    23221 gccgaaacgg caacgtccat aaaatcaaaa cgatgctgca gcagatgtat gccctcatgg
    23281 aaaaaggctg gcacattcaa tccgccgata tcgattatgc gcatcgtatt ctgaccgagg
    23341 acgttacctt cgagctggcc agtattttcc tgaacaccgt ttttatttcc tcgcagaaac
    23401 gcgtgatcac tcccaaaagc atcgcccagc ggctttacat tgaatatatg agaaatgccg
    23461 acatggtctt ctccatcggc ccggcaggca ccggaaaaac ctatctggcc atggccatgg
    23521 cggtgtcctg ccttatggaa agaaaggtcc aacgcattat tctcgcgcgt ccggccgtgg
    23581 aagccggtga aaggctcgga tttttacccg gagacctggc ggaaaaggtc aacccttatc
    23641 ttcgccctct ctatgatgcg ctctacgaca tgctggatat ggaaagggcc accgctctga
    23701 ttcacaaagg tctgattgaa gtggcgccgc tggctttcat gcgcggccga accctgaatg
    23761 actcgtttat tatactcgat gaagcgcaaa acaccacgtc ggaacagatg aaaatgtttt
    23821 tgacccgatt gggcttcggg tccaaagccg tcattaccgg cgatatcacg caggtggacc
    23881 tgccaacgga aaaagtatcc ggtctgatcg aagcggaaaa cattttgaaa aaaagcgccg
    23941 gcatccgttt tgtgcatttt tcggaaatcg atgtcatccg gcacccgctg gttcaggaca
    24001 ttatcaaggc ctatgcccat ctgactaaat caaaaaatac caatcatcat aacgtgtgat
    24061 catgaaatat tttgaatcgc tcagaataaa aatgacggaa tttttcagga actatctgga
    24121 aaaagcagca ccttatttcg gtttcgtgag aaacagcctt ttccagcgct ggatgatcgc
    24181 cgtggtgctg tgtctgattc tggccctcat catggcgccg gaactgaatt tctccaaacc
    24241 gcaattccgt atcggcatga ttgctccaaa gaacattaag gcggacttcg cttttctggt
    24301 ggaggacccg caggccgccg aggagaaaaa aatccaggac gcggaaaata tcaatcctat
    24361 ttacaattac aacagtcaga ttgccgataa tatccaaaaa aagatcgtca aggctttttc
    24421 tcttgccgcg gagcgataca acaatgtccc tgaaaaacag cagcagcccg acaaaatcat
    24481 ggatctgcaa aaagagaaaa aacgtctgga gaacaatctg ggcgcctatt tgacgccgga
    24541 agaattctat attttgaacg aatacagatt ttcggaggag ttgcagaata aattttccca
    24601 actgatcatt tccttttatg acaaccggtt tatcgccagc gatgctttcg gcaaatcgga
    24661 aaagcagaaa ggcgtggtta tccggaattt gagaacccag atttccgaag acatcaggga
    24721 cacgtctgcc ctgctgaaca tgaatgaaat cgacgaccct ctgcttaaaa ccatcaacat
    24781 gattttccgg aatgatgaga aacccttgaa agatgccgca ttctccatca cgaaaaaaat
    24841 tattgaaccg aacctggcgt ttaacaaggg cgccactgag aaaaagaaaa tggcgatcat
    24901 cggtgatgca gccgccacgt ttttccaggt acaaaaaaat gagatgatcg tccgggaggg
    24961 agaaaaaatc ggctatctgg aactggccaa actaaacgcc tactttaaag acgatcagga
    25021 caaccgattt acccgagtcg cgatcgtgct gggatttttc ttcacggcca tgtttctttc
    25081 catcgtgctt tatctgtggc gcacaagaaa ctgggtcaaa acgtccgacc ggtccaacct
    25141 ggatttgctg gttttcggca tcatcgccct gctgcaaatc ctttttatca aaatcggaat
    25201 ttttatgtcc atcgcgatca accggtcgtt ctcttttata ccggtggatg cctgttatta
    25261 cgccatcccc tttgccgtgg gcgccatgac gatcgctatt ctgatcaacc ggaatgtcgc
    25321 gctgattatc agcgcgctga cgtcttttct catcggattc ctcttcgatg aaaaaatcgt
    25381 tttcccgctg ttttcttttc tgggatcgat tgccgcatcc tatcagatcg tgcgcatccg
    25441 tcagcgttcc gtcttcctga agatcggtct ctttctgggc attatcaata tggcggcgat
    25501 tgtctgcctt aatctcatca cgggaaattt tttcaatgat ctgccgatca gattgatcat
    25561 gggattcatc ggcggtatcg tgaccggcat tcttgtggcg gggatcacgc cgctgtttga
    25621 aagcctgttc ggttttatta cggatatcaa actgttggag ttggccaatc tcaaccagcc
    25681 gctttttcag caaatgatta ttgaagcgcc gggcacatat catcacagca ttatcgtttc
    25741 gtctctcgcg gaatcggcgg ctgaagcaat cagcgccaat gctctgctta ccaaggtcag
    25801 cgcgtactac catgatatcg gcaagctgaa aaaaccgcag tatttcattg aaaaccagcc
    25861 gaactcggat aaccggcacg acaaattatc gccgaaaatg agcagcctga tcatcatgtc
    25921 tcacgtcaaa gacggctgcg aactggccgc caaagccaaa ctgggccgtc aaattatcaa
    25981 catcatccgt cagcatcatg gaaccagcat tatcagcttt ttctatgaca aagccaagaa
    26041 ggacaagaat gaatcccttc gctccctatc ggaaaacgat tttcgctacc ccggccccaa
    26101 accgcaaacc aaagaagccg gtctgatcat gctggccgac gtcattgaag cctcttcacg
    26161 caccctgacc aatccgaccc cggccagaat caagtcgctc gtgcgggaaa gaatcgaagg
    26221 aatttacatg gacggtcaac tggatgaatg tgaattgacg ctcagcaatt taaataccat
    26281 tgcggagacg tttaccaaaa tcctgaccgg catcttccat catcgtatcg attatccgga
    26341 agcgccccag aaggaaggca acgggaagaa agagtccaat gaaaatacaa atagaaaaca
    26401 ggcaaaccct gcacaagatt gaacgcagaa aaatccgcag cgccgtttcc aaaatcttca
    26461 aaatcctcga atgtacggac cggcacatca gcatcctttt tacggatgat gaacaaatcc
    26521 gggaaataaa caggtgctat ctgggaaggg ataaggcgac caatgtcatt tccttttcca
    26581 tgcgcgaagg cgagtatgga catatcaatc cccagatcct gggcgatgtg gtgatttccg
    26641 tggaaaccgc ccggcgggac gccatccggg caaagttgat gttggaagaa gaaattgatt
    26701 ttcttttaat ccacggcatt ttgcatctgc tgggttacaa tcatgaaaat acgtcccgga
    26761 aagaggcaaa aatcatgtat gacaaggaaa gggacgtctt tcgtcaaatg aacagccgca
    26821 aaaatatgcc ggcacaataa tcattatttt cacaggaggc aacaccatgc aattatcgtt
    26881 ggaaaataaa gtggcgctgg tcaccggcgg cagcagaggc atcggcagag caatcgcgtt
    26941 gggactggcc aaatccggag cggatgtggt gatcgccagc cgcaaaatcg gcgatctgga
    27001 aaacgtggcc ggagaaataa aatcgctggg aagaaagtgt cttccggtag caacgcatat
    27061 cggccgtctg gaagaaataa aaaatcttgt cgacaaagcc gctgccgaat tcggcagaat
    27121 cgatattctg gtcaacaatg ccgcaaccaa tccgaccatg gtgccggcga tggatattga
    27181 cgaccgcgcc tgggattcca tcatgaatct gaacctcaag gggttgtttt tcttgagcca
    27241 ggcggtggcc cgtatcatga aggaaaaagg aggcggacgt attatcaacg tagcatcggt
    27301 tgccggtctt tctcccgata tcctgccggt ttattccatc agcaaggcgg gcgtcattat
    27361 ggccacgaaa gtcatggcgc aacaatgggc gatctacaac atccgggcca atgccatcgc
    27421 acccagtctg accaaaacaa agttcagcga accgttatgg accaatcagg atatcctgaa
    27481 tatcgccatg agcagaacac cgctggggcg tccggcagaa ccggacgata tggtcggggc
    27541 cgtcatcttt ctggcatccg atgcatccga ctacatcaca gggcaggtgc tcgccataga
    27601 cggcggcatg accatttgag gccatctcga ttttttcacg ctgtttaccc gaaagcatcc
    27661 ggtttatacg gagggatcaa gcaccggtgt gtgcgtgatc aaaaaaatat tgaactttcc
    27721 aatatgataa agtattataa tcaaatgcaa aataatattt accggctttt caaacttcat
    27781 ataacattat tttccaaaat cgtcatcgcg gcactgttat tttttttcgc tgccgcgttc
    27841 acggacatag cccgtgccga tatctacaaa tacgaagata atgaaggcgt gttgcatctg
    27901 accaatgttc cttccgatac cggcgcaaaa tacgttatgg tcatgaggga aaaacgcatt
    27961 ctgtttcagc cccagctcga cgttaataaa tacgaccaca tcatcaataa aaccgccgat
    28021 aaattcaaac tggactccgc tttaatcaaa gcaatcatca aggcggaatc gaatttcaat
    28081 caccgggccg tgtcccccgt aggcgctcag ggcctgatgc agctcatgcc gaaaaccgca
    28141 tcgcaactga atgtggatga cagttttcat cctgaaaaaa acatcgaagg aggcgcgcgg
    28201 taccttcgat accttctcaa tttatacaaa ggcaatctgt cgctggctct cgccgcctat
    28261 aatgccggcg aaggcgccgt ggccaaatac aactacaaca ttccgcctta ccgggaaaca
    28321 caaaattatg tcagacgcgt cctgagtttt tacagatcgt tcagcggcaa ctgattcaga
    28381 cgtttttctt ttccacgtac agatatttct caatatgttt cattctcttt tccttgatgc
    28441 ccttgatctc catcaactgc cggatgtcgc tgattccgtt gagccgcgtc cgcaattcca
    28501 gaattctttt cgccgtcacc ggtccgatgc ccttgataag aatcaaatct tcttccccgg
    28561 ctttattaac atcgatgggt aatccgacag agattttcgc tgtgttttgt atttccgcca
    28621 ccgtaatacc cggacggtcc gatgcggaac gaatcaccag cttcatcccg gttttgacgg
    28681 taaagtcctt ttcgaattgc ttttcgacgc ccgcggtttt cagcaggcga ttgaccgaca
    28741 tccccgatgg aacgtggtaa attccggccc tttgatcatc gccgacgatc tcgatcacac
    28801 agcagtgatc gcacgtgtcg gcataaacgg gaatccggta aggagtccaa gaggtgatca
    28861 tcagagaaac aaaggggatg accgccacca taaggctcaa aacgatcagc cccttcagtc
    28921 tgctctctga aataaataca atgttatttt ttcttatcca gtccgaaagc atcgtgcagc
    28981 accgacaccg ccagttccat gtatttgcgt tgaatgatgc aggatatctt gatttccgat
    29041 gtgctgatca tcataatatt cacgccttca gccgccagcg ttttgaacat cttcgcggcc
    29101 acgccggaat gactggccat gccgacgccg ataatggata ttttggcaac ctgatcatcg
    29161 acttcaattt tcttcgctcc gacttttttc gccgtgcttt caacaatttt ctgcgcttcc
    29221 ttcaaatcct ttttggatac ggtgaaagtc agatccgtat agccttccga actggcattc
    29281 tgaataatca tatccacaaa aatattttta tccgacaacg ggctgaacaa agacgccgcc
    29341 actcccggct gatccggcac atgcacaacc gtaatcttga cctgatcccg atcatacgtc
    29401 acacccgaaa tgatttctct ttccatatct ttatcctccc tggtcacaag cgtccctcct
    29461 ttatccgaaa atgttgattt gacataaacc ggcacattgt attttttagc catttccacc
    29521 gaccgcggct gcaggacttt ggcacccatc atcgccatct ccagcatttc atcgtaggaa
    29581 atcttttcca gccgtctggc tttgttgcaa acgttcgggt cggtggtata aacaccgtca
    29641 acatccgtat aaatgtcgca ctgatccgct ctcaatgccg ctgccagcgc cacggcgctg
    29701 gtatcggacc cgccgcgtcc cagcgtggta atgttattgt ctttatccac cccctgaaac
    29761 cccgcaatca cgatggcggt tcctttttta agttcctttt tgatgttttc cgtatcgatc
    29821 agcgaaattc tggctttctt ataagcctga tcggttaata ttttcacctg aaagcccaga
    29881 aacgatctgg ccttgcagcc catcgcattc aaaacgatgg ccagaagggt tacggtcacc
    29941 tgttcgccgg tggaaatcag ggagtcatat tctctttcat cgggcaaatc cgtcgcactg
    30001 tgagccaggt taatcagcct gtcggtctcc cccgccatgg cggacaaaac aacgacaacc
    30061 tgatggccgg cctgtttgac cttgatcact ttttcagcca cgttttttat tttctcaata
    30121 ttcgcgactg atgttccgcc gaacttctga acaattaatg ccatatttaa acaccctccg
    30181 tttttatatc tttgatcaac tcgtttaacc tgtctgcgga cgccctgatg atcatttttc
    30241 tccggttttc atcaacgtat tcaaaatcga tgtaaaccgt tttttccggc agaatctttt
    30301 tgatcttctc ggcccattct ataacaacaa cgccttctgt tccaagatat tcttcgcatc
    30361 ccagatcgtc gagttcagaa taactgctga tccggtaaac gtcgaaatga taaagcctat
    30421 gacgcgccgg atattcatta atcaatgtga acgtcgggct ggtgatcctg tatttttcat
    30481 ccacgcccaa accgcgggcc agccccccgg taaaacaggt ttttcccgaa cccagctccc
    30541 ccgaaagcgc gaccacgtct ccatcctgga gcccttctcc gatagagcgc cctatttcca
    30601 ttgtattttc agtacttacg gatattatct caaccgtttt atcatcctga ctcatcttgt
    30661 catttcaccg gcttttaatc gattgtttcc agtaaaacca tggctgcgga atattttttt
    30721 gtatgcgtca gactcaaatg aatcttgtgg atccttcttt tttgaatgta ggattgagca
    30781 tcgccggaga gattcagaaa cggctggccg ccttcatgat tcacgacgga aatgtcgctt
    30841 agttttatgc ccattcccaa acctattccc agcgctttca ggaatgactc tttggcggca
    30901 aaccgcgcgc tgtagtgcgt caccggataa gcatgtttgc cgcagtaggc catttcctcg
    30961 gtggaaaaga ttctgtttaa gaacttgaca ccccatcgat cgatgattct ttccatgcgt
    31021 ctgttttcga caagatcgac ccctattccg taaaccatca gatcaccttc cggggatgtg
    31081 tcatcgcgtc cgtcaccgca gccacttttt tcatggccgc cacgtcgtgc acccggacga
    31141 tgtgacatcc gttcataatc gccgcggaaa ctgtcgctgc ggtgccttcc agcctttgcc
    31201 gcggttcatc ccctgtcaca ttcccgataa aggacttgcg cgatggtcca accagtatcg
    31261 gcatccccag agttctcagg tctgcaagcc tcctgatgat tctgtagtta tccgcaaccg
    31321 ttttgccgaa accgattccc ggatcaatca ccattgcgtc tttgctcacc cctgcccgga
    31381 tggctttccc gatactggcg ctcaaataat ccgtgatgtc tcccagcaga tcatcatagg
    31441 ccagatttcc cttctgcata ttttccggat tgccccgcat gtgcatcaac acaagagcgg
    31501 cgcccgtttc cgcagccgtc ccggccattt tacgatcgcc gttcagggcg ctgatgtcgt
    31561 tgatgatttc cgccccggcc tcgacagctt cccgggcaac acgcgctttt ttcgtgtcga
    31621 tggagagggg aatctttatt ttccggctca acgctttgat cacaggaagg actcttttga
    31681 gctcgacatg aaccggtact gcggcagaac cgggacgcgt tgattctccg ccgacatcaa
    31741 ggatatccgc tccttccgcc tccatctgca atccatattc aaccgccctt tccgctgata
    31801 gaaaggtccc gccgtcggaa aacgaatccg gggtgacgtt cagaattccc ataatccggg
    31861 ttctgtcacc cagcgtgatt ttcctccggg atgtttttaa gataaaccgt ttctgcccgg
    31921 cgtttttcag aatccgcaaa atatcccggg cgatgctatc cagaccaaaa ggctgccggg
    31981 ctatcttcgc cgccagccgg gagagctgtt tcagcgtccc catcattaaa acatcggtcg
    32041 tttccacgct gcaggcgaca cttcctctgg caaccgccgc atcaccgccg agagccagca
    32101 tttcctgctt caggatattg gcaattttac acggctgttt ctccagcagg atattcagat
    32161 tgaccgcttt gggcgccatc gcgccgatgc cgtaggcatc caccccgatc cgcctgaaca
    32221 gcgcagccgc ctcttgtgtg ctttgaatat caattatctt caccagaaat caccaaattt
    32281 caagattccc aatattatga acggatggtt gaagtgatat agtgatcaga ctgaagtctg
    32341 cggctcgctc ctgtgtgcct tgggtaacgc atcgccgatc agggtgtcta tttccgcgcc
    32401 gcttaaaact tctttttcca gaagttcacc cgatatcttg tggagaagat caatatgatc
    32461 ggtgagaagc tgttttgatc gttcataatt gcgcgctaca atggccatga cttccgcatc
    32521 gatgtttttt gccgtttctt cgctataatc cttgtgcgtg gcaaattccc gtcccagaaa
    32581 gatctgctct tcttttttgc cgtaactcaa cggccccatt ttgtcactca tgccccattc
    32641 acagaccatt ttccgggcca aatttgtcgc gcgttcaatg tcgttgccgg cgccggtggt
    32701 gaagtctttc aagaccagtt cctcggcagc tctgccgccc agcaaaatgg aaatgctgtt
    32761 ttccagatac cgtctcggat aggtatgctt ttcatccacg ggcagctgct gggtcagtcc
    32821 cagcgcgcga ccgcggggaa tgatggtcac tttatgaatg ggatccgttc ccggaatcag
    32881 acgggcgacc agcgtatggc cgatctcatg atacgaggta ttgcgtttct cttcatcgct
    32941 gatcaccatg gtttttcttt ccgagcccat caggatcttg tctttggcaa actcgaaatc
    33001 gctcatgttc accttttctt tattggcccg ggcggcgttg agcaccgctt cgttgatcag
    33061 attttcaata tcggctccgg acgtgccggg cgttccccgg gccagaacgg caaaatccac
    33121 atctcccgca atcggggtct tgcgggcgtg cacctcgaag attttttccc ggcctctgac
    33181 gtcgggcagc ggcacgacaa cctgccggtc aaaacgtccc ggccgcagca acgccggatc
    33241 gaggacatcg gggcggttgg tggctgaaat caagataacg ccttcattgg attcgaagcc
    33301 gtccatttcc accagcagct ggttgagcgt ctgttccctt tcgtcgtggc cgcctcccag
    33361 accggcgccg cgatggcggc cgaccgcgtc gatttcatcg ataaaaataa tacagggagc
    33421 gttttttttg gcctgactga acaaatcgcg aacacgtgag gcgccgacgc caacaaacat
    33481 ttcaacaaaa tccgagccgc taatggtcaa aaacggcact tccgcctctc cggcaatggc
    33541 ccgggccaga agcgttt
//
LOCUS       DAOOLB010000019        32760 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_1135_length_32760_cov_38.585201, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000019 DAOOLB010000000
VERSION     DAOOLB010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 32760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32760
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1135_length_32760_cov_38.585201"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="PLF77_04185"
     CDS             <1..1262
                     /locus_tag="PLF77_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HPC08088.1"
                     /translation="IVEIDNAMKWGYNHQLGPFESWDAVGVKEAVAVMKKLGKKVPPK
                     IEEMLKNKCTSFYKVDKKGVKSYYDFKKKGYVKLDENPKIILLPGLKERKKTIKENPS
                     ASLVDIGDGVACLEFHTKMNSIDPDMMKMISEGCDIVNKDFTGLVVANHDQRMFSAGA
                     NLFLVLVAIQEGKWDELKTMVRELQIVNMKMKYLPKPVVIAPAGMALAGGCEIAMHGD
                     YCLANGETYMGLVEVGVGVIPAGGGCKELAIRATEGIPAGVVESGLNMQYHFGKIFEN
                     VATAKVATSAQEAQDLGYIRKTNMINMSRDMQIYEAKQIVLGLSKFYKPPKPALIPVM
                     GENLRGLAGAILSNMKAGRYASEYDVHIAMKIAHVLGGGTCAEGTWITEQEMLDLEIE
                     AFMSLCGEQKTQDRISAMLTTGKPLRN"
     gene            1341..2534
                     /locus_tag="PLF77_04190"
     CDS             1341..2534
                     /locus_tag="PLF77_04190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPC08089.1"
                     /translation="MSEAVIVASVRSAGGRYKRGGLSETRADVFGVQVLKGLLAKVPQ
                     LDPKDIDDVIVGCAFPEAEAGMNFGKVLAVGAGLPEPVSGMVLNRFCSSGVQSIADAT
                     AKIRAGWSDVIIAGGVESMTHIPMGGGIFRPNPDWEWDTMPNVYMNMGNTAENVAMNH
                     GVSREDMDKFGLESNRRAYEAIKAGKFKDEIIPIKAFKYKKDKEGKRIRTEFVFDTDD
                     GVRWPSDLGAMGKMKSPFKQGGNCTAANSSQMTDGAAFTLLMTPEKAKAIGVKPIAKL
                     AYYAVAGCKPEEMGVGPRYAIPKVLKLAGLTTKDIDVFEINEAFASQALYCIRELGIE
                     DRLNAGDINPNGGAIALGHPLGCTGAKLTAQLLHEMKRRGSKRGIVSMCIGGGMGAAA
                     IYEML"
     gene            2755..2829
                     /locus_tag="PLF77_04195"
     tRNA            2755..2829
                     /locus_tag="PLF77_04195"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2787..2789,aa:Val,seq:cac)
     gene            3149..4360
                     /locus_tag="PLF77_04200"
     CDS             3149..4360
                     /locus_tag="PLF77_04200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPC08090.1"
                     /translation="MALRDAYFVDGVRTWFGKSRQDGHYWTTRADDLVTKVMKELIRR
                     NPKVPWGEVDDNIWGATTQVGDQGTTMGRVTVLTAGLPVEVPGFSVDRMCAGGMTCQA
                     IGSSFVKTGCADIIIAGGVEHMAHHPMGAGADPNPRVVTEKMVEVKYFNMGNTAEKLH
                     DWLPENGEQPVTKEEADEYAYHCLEKYFKALDAGYYHKHCVDMTVFTPNGWKVANYDE
                     QAKRGTTLESIKNFRKFPFKAEGHVTAANASGLNDGACVSLIMSGKRCKELGIQPKMK
                     FVGAGFVGVDPSIMGWGPVPSTEKVLKNFNMKFDDLDIIELNEAFAVQAVGFMKHFGM
                     KLPQDPRLNPYGGTIAMGHPLASSGVRLSMQLAQDFELNPKAKYGLTTMCVGLGMGGS
                     IIWENVVGKKL"
     gene            4373..6454
                     /locus_tag="PLF77_04205"
     CDS             4373..6454
                     /locus_tag="PLF77_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPC08091.1"
                     /translation="MAKYITECKLNFLQSAKAGKIAIVTMDNGQFYNIPNTWGFEAFE
                     FLNKIIDTVEKDKDVKGWILTGKPFIFNVGADIMSVDPTATKEDLLKIGEMGHGTFKR
                     IMNLKIPTLAAINGAAMGGGVEVGLYHNYRTISKSPGGCDHYALPECFLGLIPGWGGT
                     QLVTKLCGPEAAIELIINNPLNMNKMINSIKAVEMGLADRLIAPAEFMDESIALLERI
                     ITGEEKIERKKVDPKMSDELYNKTKEFITGKVHSGAIAPYKALELIKGAAEWSYDEGF
                     QKETEVLADMIMSDQFKCGVYSFDLTQRRAKKLPGRPPKEVKGNKIAKIGVIGAGLMA
                     SQMALLFAQRYQCPVIMKDIKQEFIDKGLAYCREQLAKTAKKGKLSAADAKWIGEDLI
                     SGTLTYEPFKDCDFVIEAVFEEMGIKQKIFGELEAACKPECLFLTNTSSLDVAEMGKN
                     LKDPSRVVGFHFFNPIAVLPLVEIIKTDKTSDVVLATAFDIAKKIKKTPVLVKNAPAF
                     LVNRVLLAWMDGIFQCIDEGADFMQVDNAVFDLGFPMSPFTLAALVGPAIALHVQETL
                     NKSFPDRFPVNAGLKNLVAKGKKQMVTFTDKGIDVDPEIKAGWPQGNKKFTDDEIKAR
                     VLERVAKEVDLILAEGVVAEPKDVDTGVIMGAGWPFFMGGITPYLDQKGISKKVAGKD
                     FHPKGFLTVEK"
     gene            6780..7043
                     /locus_tag="PLF77_04210"
     CDS             6780..7043
                     /locus_tag="PLF77_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08092.1"
                     /translation="MPPAEMRHERVVVETDRYRVEGDITLPQEGYRSRLSDYLNRSDQ
                     EFIILVNVELLSLDGSGRDWRAPVLMLARQHIRLVVPVGGPNA"
     gene            7253..8038
                     /locus_tag="PLF77_04215"
     CDS             7253..8038
                     /locus_tag="PLF77_04215"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HPC08093.1"
                     /translation="MNVKDLFDLKGKISVVTGGGRGIGKFIATGLAEAGSDVVIASRK
                     MENLEKEVKELENLGIRALAVKCDLAKKEDIGNLVKAAMDKFGTIDILVNNAGLTWGA
                     PTLDYPLDKWEKIFDVNVKGVWILSQQVARIMKEKGGGKMINVSSIYGSRGSNEEEHP
                     AVAYNPSKFAVEGLTKTLALKLARYKIYVNCIAPGFFKTDMMGHLFKPEMKPILDLTV
                     GAIPLKMYGEEDDVKGLAVFLASKASNYITGGIFPVDGGFSAQ"
     gene            8115..9215
                     /locus_tag="PLF77_04220"
     CDS             8115..9215
                     /locus_tag="PLF77_04220"
                     /inference="COORDINATES: protein motif:HMM:NF018805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HPC08094.1"
                     /translation="MKKIKHSFFLLLSLIVVACAPAYTLNHSSPVKPLSLPADAAAHY
                     WAQNEWWYYTGHLKAEDGREFGYELTFFKRITSEDKVPHFFIPIPAHWLKDLGMMGHF
                     VITDITHQKFMTQEVNNFFGLPSKADDTKYDLMIGTWTAREEEGKHLLYAEMDDGRIE
                     LELQPVKVAALHGRQGIVDKGGGNSNYYYSLTNMKTSGTLTIGGESLDVHGKSWMDHE
                     YGTMKLTYPQSGWDWFSIQLENHYELMIYVIRSEGDLKNSLVGGTFVLPNGKTIPVKA
                     RDIEIRNLDYWHSEKTDSDYPSSWEIIVRPFKLKLKCQPVMAEQEVNLQPMPYWEGAI
                     SVSGTFHGKSVKGQGYIELVGYSRKYPIQYIN"
     gene            9353..10102
                     /locus_tag="PLF77_04225"
     CDS             9353..10102
                     /locus_tag="PLF77_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPC08095.1"
                     /translation="MSGHSKWSTIKRKKGAIDAKRGKIFTKIIKEITLAARLGGGDIE
                     GNSRLRQAVMLAKEENMPKDNIERAIKKGTGGGEGAAAYEEVTYEGYGPGGAAVLVEI
                     MTDNKNRTVAEIRHIFSKHGGNLGENGCVAWMFAKKGSIVIDKKVIDEDALMELVLEA
                     GAEDVKTEDSEYEVITDPGSFEAVKKILDDKGIKHLEARIGMIPSNTVKLEANKAEQM
                     LKMMEKLEDNDDVQNVYSNFDIDEEVMKKLS"
     gene            10138..10623
                     /gene="ruvC"
                     /locus_tag="PLF77_04230"
     CDS             10138..10623
                     /gene="ruvC"
                     /locus_tag="PLF77_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPC08096.1"
                     /translation="MTLRILGIDPGSHVTGYGVIEKKGNYLKHVVHGEIKPKRNSSLS
                     EILVYIYQQLSSLIAQTVPQAVALENIFYGKNVRSLIKQAQVRGVVIFAGADKDLNIF
                     EYSPLEVKKAVVGYGRAEKRQVQVMVKAILNLPETPPPDAADALATAICHANFLKDKP
                     I"
     gene            10623..11231
                     /gene="ruvA"
                     /locus_tag="PLF77_04235"
     CDS             10623..11231
                     /gene="ruvA"
                     /locus_tag="PLF77_04235"
                     /inference="COORDINATES: protein motif:HMM:NF001040.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPC08097.1"
                     /translation="MIALISGTIAYKGISQIIVDVRGVGYRVFIPLTTFYELPETGQT
                     ISLHIHTNVKEDAIHLYGFHTLQERDLFQLMISVSGIGPKVAMNILSGIAAPELLRAI
                     SGGNVSKLVNVPGVGKKMAERLVLELKEKVLKKMAIEEAPEDQTQHLADDLMQEDVLS
                     ALMNLGYRNNVARDALNKVLRTSEAKLSMDKILKETLKILAG"
     gene            11254..12279
                     /gene="ruvB"
                     /locus_tag="PLF77_04240"
     CDS             11254..12279
                     /gene="ruvB"
                     /locus_tag="PLF77_04240"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPC08098.1"
                     /translation="MEKSKKNSLISPSCAEEENDFEVTLRPTMLKDYVGQEKVKSNLA
                     IFIEASRKRREALDHVLLYGPPGLGKTTLAYIIAREMNVDIKVTSGPVIERPGDLAAI
                     LTNMHENEVLFIDEIHRLSHVVEEILYPAMEDFHIDILIGQGPSARSMKLDIPRFTLV
                     GATTRAGSLTSPLRDRFGMHFRLEFYSPKELAVIIKRSASILGVKLTASGAEELAGRA
                     RGTPRIANRLLKRVRDYAEVKADGTIDGKIAQEALTAFEVDQKGFDQMDRRLLLTLIE
                     KFNGGPVGIESLAAAIGEERVTIEDVYEPFLIQEGYLQRTARGRIACSRAYEHFGLKA
                     SGQTEIF"
     gene            12314..12856
                     /locus_tag="PLF77_04245"
     CDS             12314..12856
                     /locus_tag="PLF77_04245"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HPC08099.1"
                     /translation="MKILMHICCGPCTIYPLKELRTQGHEVTGVFYNPNIHPYQEYKK
                     RYQTLSQYADQIYLKIIWPEDYLMEEFLKGVAFKGKERCSYCLQDRLTFTAQMALQEK
                     YDAFTTTLLYSKYQKHDFIHQAGDNLSQKMGVSFYYQDFRVGWEEGVKISKELEMYRQ
                     PYCGCIYSEKERYYKQNKHS"
     gene            13049..13978
                     /gene="lpxC"
                     /locus_tag="PLF77_04250"
     CDS             13049..13978
                     /gene="lpxC"
                     /locus_tag="PLF77_04250"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HPC08100.1"
                     /translation="MNLQRTIKKEINCFSIGLHTGRKVNMKIKPAPADTGIVFIRKDL
                     PEAEPIYARYDNVCDTTLATTLGSNGITVSTVEHLLSAFSGMGVDNAVIELDSFEVPV
                     MDGSALPFVNMLKEVGTHVQKKHKKMLVVKKSVCVNAGDAKAQLLPSEQFKITYEIDF
                     KHPVIGVQSYSMTFSDEEYEKNICAAQTFGFLKDLEFLQARGLALGGSLKNAIVLDDQ
                     KIINKEGQRIPDVFVKHKILDAIGDLFLLGMPIIGHFVAYKSGHRLNNLLLRELISRR
                     DCWEIVTDVNKAENLKSHPFKIPSFRTLDAVQA"
     gene            14138..14707
                     /locus_tag="PLF77_04255"
     CDS             14138..14707
                     /locus_tag="PLF77_04255"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HPC08101.1"
                     /translation="MKKIKNKIGLVLIFLFMGAMFFPSQTCALEPKIKDLLITNDAEN
                     VLLYARLLNGFKPQMELAILAGIPTTFTMWIEVYQERSPMWDKKIIRKEIKRSIKYDN
                     LKKTFSVVSEKKDPDIFSDMESAQKAMSDYNGIVAVPMSSLKKGQSYYTLVKIKMDKV
                     RLPLHMEYVFFFVSLWDFETPWYRQNFTY"
     gene            14761..16980
                     /locus_tag="PLF77_04260"
     CDS             14761..16980
                     /locus_tag="PLF77_04260"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08102.1"
                     /translation="MENKPGRSIIDEKELSKRRRERIIMVVVGVLAIIVTLMASQLFN
                     KENLPLSTNIFVYGLTSINFILIVLLMFLIIRNIVKLFYEHRRGVIGSRLRTKLVAAF
                     VGLSLVPTILLFLFAINFLSYSIDFWFNIKIGDALNRSLEVAQVYYQQMEEHAKFNAR
                     QISADITKNRLYEQDRVEYLKSFVTQRQKNYKAGMVETFFDFQQEKLTFADAEDPDIL
                     PVDPTPKMMENIYLGKEESIIHPAEKGDVIVGIVPVFSYAVPTEVIGRVSVSYMIPKG
                     FVDKLRSIADASEQYRKIELLKKPIKLNYMVTLSIVTLVIIFLAIWFGLTLSKGITGP
                     VQDLVQATNRITQGDLDTRIDIDADDEIGTLVKSFNQMTKDLKKSKTSLEEANLNLEQ
                     RRKYMETVLRNVSAGVISVDKTGIITTINRAAEHMFDIRSEKILFRNYRELMIPEHIS
                     LVDEFLQEMKEKNESVLEKQLEIMLQEKALTVLLTITTIQDEEGNDSGIVVVFEDLTL
                     LQKAERAAAWREVARRMAHEIKNPLTPVQLSAQRLQRKYEDKLGEDGSVFKECTQTII
                     DQVEVLKNLVNEFSRYARMPVTSPSPNDLNAVVSEAVSLFQDAHKDIVFDLKTADGLP
                     KFDLDAEQINRVMINLLDNAVAAMNKKNGHIEIAVSYDEQHRRASVSVADDGPGVPSS
                     YKRKVFEPYFSTKKSGTGLGLAIVSSIISDHHGQVNVSDNSPTGTIVSFQLPVSKAS"
     gene            17121..18491
                     /locus_tag="PLF77_04265"
     CDS             17121..18491
                     /locus_tag="PLF77_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC08103.1"
                     /translation="MKETILVVDDEESICQSLKAILSDEGYQVLVAGSGEEAVKVVEE
                     EMPRLVLLDIWLPGMDGLDTLKAIKKIDAQTMVIIMSGHGTIETAVKATKLGAFDFIE
                     KPLSLDKIIILVNNALSLIRLKEENILLKQKVTHQYELTGNSPVIVDLKEMISIVAPT
                     NAWILIMGENGTGKELVARSIHRLSLRADKDIVEVNCAAIPEELIESELFGHEKGAFT
                     GATEKKRGKFDLAHEGTIFLDEVADMSLKAQAKILRILQEKKFERVGGNRLIDMDVRV
                     LAATNKDLEEEMKAGRFRQDLYYRLHVIPLVVPPLRDRKEDIPALAERFILDFTRKEG
                     MEPKILSDDAIELLMKHDWPGNVRELKNIIERMIILTPSNDITAKDIPPLNTKEAKSV
                     FPGTPVAAADSFRDAKMDFERQFILKKLEENDGNISRTAEAIGMERSNLHKKLRSLNV
                     VIKGDA"
     gene            complement(18488..19474)
                     /locus_tag="PLF77_04270"
     CDS             complement(18488..19474)
                     /locus_tag="PLF77_04270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPC08104.1"
                     /translation="MNKKVGFGILISALLVYLSVRGISLQDVLNDLNKIELTYVILFL
                     FLIILMQYLRSYRWGVILQPLEKIDQVSLFSVTSVGFLAIAAIPARMGELARPYLISQ
                     KSSVKMSSALGTIIIERVLDSFTVLGIAILLLFFIDLPSWMIHSSIIFFLLALAIFCF
                     ILFMILRRKETLQLVNKVLAMLPGRLADKIDELLYNFTNGLQIVTNINLLLYLIFLSV
                     TVWLLDVLAIYVLFLAFGFHLPVIASFVLMIILIVGIAIPTAPGFIGNWHFACVLGLS
                     LFSVAKVEALSFAVVYHFLSMIVVVVLGVIFIPFNKFSISEMKNQINNEKQV"
     gene            complement(19506..19910)
                     /locus_tag="PLF77_04275"
     CDS             complement(19506..19910)
                     /locus_tag="PLF77_04275"
                     /inference="COORDINATES: protein motif:HMM:NF023514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3568 family protein"
                     /protein_id="HPC08105.1"
                     /translation="MKRNVFLSFISCILIPFLFSGCVPLMVGGAAAGAGAGTYLYING
                     ELKTDYNKSFDQVWKACEKTIAGMHGIDVAPEKEIARGTIQTIINDEKVKFDIAYKAK
                     NLTTVAIRVGVIGNKLSSQLIHDRIADNLVKE"
     gene            complement(20007..21071)
                     /gene="dnaJ"
                     /locus_tag="PLF77_04280"
     CDS             complement(20007..21071)
                     /gene="dnaJ"
                     /locus_tag="PLF77_04280"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPC08106.1"
                     /translation="MTKKDYYDILNVPRTASEDEIKKSYRAIAMQCHPDRNPGNKKAE
                     ERFKEAAEAYEVLSDREKREIYDHYGHEGLNSTGFRGFSGFDDIFSHFSNIFDDVFGY
                     GNARGRSHSYATAGADLRYDLTISFLDAAFGTTTAIDLEKLTTCHHCQGTGAAPGSSP
                     EICRTCRGRGQVIQSSGFFTISSTCPHCNGHGKVISTPCENCRGTGKDRIIKTVQLKI
                     PAGVETGSRLRLRGEGETGSQGGPDGDLYVFINVEEHEFFNRSGDDVICRAPISFVQA
                     ALGGTIEVPTLQGKEKMKIPRGTQNGKTFRLRGKGIPHIRGFGRGDQIVEVCVEIPTT
                     LSKKQEELLIEFEKLNQPTR"
     gene            complement(21347..22228)
                     /locus_tag="PLF77_04285"
     CDS             complement(21347..22228)
                     /locus_tag="PLF77_04285"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPC08107.1"
                     /translation="MIKSIQKTDFHELNLISRGKVRDIYSVGNYLLIVTTDRISAFDV
                     IMPDPVPGKGVILNQMSAFWFEKMKDIIPHHIVSTNPAEFPAETAPYRDSLEGRSMLV
                     KKATPLPVECIVRGYISGSGWKDYQRDRTISGITLPAGLKESSRLPEAIFTPTTKAPE
                     GEHDSPISRKEMADIIGAELTASVINISLAIYQRAASMALKAGIIIADTKMEFGLLDG
                     NLMLIDELLTPDSSRFWPADEYKEGHAQKSYDKQFLRDYLLSINWNQKPPAPQLPSEI
                     IENTRKKYEEALKRLVG"
     gene            complement(22244..22873)
                     /locus_tag="PLF77_04290"
     CDS             complement(22244..22873)
                     /locus_tag="PLF77_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC08108.1"
                     /translation="MFVKQMQVSSMAVFAYIVGDPITGDALVIDPADNIKGIVAEAQK
                     NNLRINYIVNTHGHVDHISGNTEMQKATGAKIIIHQDDAIMLTHTPAMILRMFGAKAS
                     PPADRLVKEGDILSAGNVELRVIHTPGHSPGGICLYTPGFVFTGDTLFVEAVGRTDLP
                     GGSWQELSRAVREKIFTLPDDTKVMPGHNYGSTPTSTVGHEKRYNPFFR"
     gene            complement(22961..24304)
                     /gene="rsmB"
                     /locus_tag="PLF77_04295"
     CDS             complement(22961..24304)
                     /gene="rsmB"
                     /locus_tag="PLF77_04295"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HPC08109.1"
                     /translation="MKKTIRHQAVEILNQVHAAHDFAGPLINDCLDKYNLSGTPDGRL
                     LTHLVYGVLRFRGHLDWLLKKLYRGDFAKMQEDIKNVLRVSLYQLRFSERLPDFAVVN
                     EGVEIAKIINPDKSSLVNAILRNFLRRGQNLSFPSEKKNPVEYLAAFHSHPEWMIQTW
                     IKTFGLQETKALCSADNTLPPLTLRVNTLKISRNDLKEKLAAAGFESQTTPYSPNGII
                     LSTAVKPIQKTEFFDKGLLRIQDEGAQLIACLVNPERNESILDACAGAGGKSSHLAAL
                     LENKGFILAFDRNQEKITELKKEIIRLGITIIETQAGNVSAGLPDAFRERFDCVLVDA
                     PCSGLGTLRRNPEIKWRIMQNDILSFAAQQKKILQQASAAVKRGGRLIYCTCSVLPEE
                     NENIVEHFLRVNNQFSLGKPSSTIIRPLTDSSGFFRTFPHRHNMDGFFGALLIRQ"
     gene            complement(24301..24903)
                     /locus_tag="PLF77_04300"
     CDS             complement(24301..24903)
                     /locus_tag="PLF77_04300"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPC08110.1"
                     /translation="MNHTMFNTPFVKSLLRGISLLLLKMLGWKTSGKIPREPKFVLIV
                     APHTSNWDLFYGILLAFALKIDARYIAKKELFRGPFSFLMSWTGAIPVERAARHHIVD
                     QMAALLKEKEKCILALAPEGTRHKTDAWKSGFYYIALKAQVPIQLAFIDYARKTGGTG
                     PLIYPTGHPEHDMSMIRDFYQSVRGKHPENTSTVGIHPES"
     gene            complement(24939..25877)
                     /gene="fmt"
                     /locus_tag="PLF77_04305"
     CDS             complement(24939..25877)
                     /gene="fmt"
                     /locus_tag="PLF77_04305"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPC08111.1"
                     /translation="MTNPRILFMGTPAFALPALRLLHGQRYPIAGVVTQPDRPKGRGL
                     KEIASPVKILAQEFGLNVYQPEKVKDPSFMELLQQLHPDMITVAAFGQILPKSVIEYP
                     RLGCLNIHPSLLPRYRGAAPINWQIINGEKKTGVTIMLMDEGMDSGDILLQEESQLDC
                     EENFGELHDRLAKLGAALLIQTIDQVISGTASREKQETTGVTFAPRLTRETGKILWTG
                     NASDIVNLIRGLSPSPAAYTFLDGLSLKIFSAVARHENVQQPPGTICAIEADSLSVAT
                     ADGYVIVKDVQLAGKKRMPIADFLRGYHLKIGNSLS"
     gene            26117..27256
                     /locus_tag="PLF77_04310"
     CDS             26117..27256
                     /locus_tag="PLF77_04310"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC08112.1"
                     /translation="MATFLFGFSQSIVDSTFNNFLNETYLIDNLQRGILEIPRELPGL
                     LVVAVSAIVFFLCSRRQAALANLLCAFGILLIGFYSPNFSVMLIWLFIFSIGQHLFLP
                     LNQSIGMELAVKGQTGRRLGQFSAVANVAMIIGSFSIFVGFQYFDLDFKTSYFAAFLG
                     FSSVAVLIFMMKKDEPVPVRMKFNLRKEYTLYYWLTILYGTRKQLFLTFAPWVLVTVF
                     HQKTQVVATLLTVGCVIGIFFKPMLGKAIDKLGERLILASEAVILVFVCIGYGFSKSI
                     FTETTALYAVFVCYIVDQMLAAVSMARATYLQKIAVRPEDVSQTLTMGVSIDHIFSIG
                     IAVLGGFIWLKFGYQYLFLTGAVIAVLNFFSALQIKTKTEKLTTP"
     gene            27295..27801
                     /locus_tag="PLF77_04315"
     CDS             27295..27801
                     /locus_tag="PLF77_04315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08113.1"
                     /translation="MKKIKVTVNDLMQQNYVYYLQEPVGKNFHPQFTPDLTPGKMLEM
                     GVFGGKYMTDCKDEFPKRWFTRAKLCHERHDPDLNYFGVTASQPLSVWREKGWIYHED
                     PRGWFQWYCRYYMGRRCSDDERQIKRWKAMKRHISQIRKHCQKGDINCRPRQRQALLN
                     WAYDSRNI"
     gene            27868..28326
                     /locus_tag="PLF77_04320"
     CDS             27868..28326
                     /locus_tag="PLF77_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HPC08114.1"
                     /translation="MTETWLRITNPDNPPNDAMLSAWIGNKAYKYLQQVTKLIVALYP
                     DVFVPEWIYGGKKHGWSLRYKKSKSFCTLIPEKSRFALLIVFGAQERANLETVKDRLS
                     QHTRRDYDRAATYHDGKWLLLPVDNDQVVEDLKILLAVKRKPLTAECIIK"
     gene            28459..29241
                     /locus_tag="PLF77_04325"
     CDS             28459..29241
                     /locus_tag="PLF77_04325"
                     /inference="COORDINATES: protein motif:HMM:NF005126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HPC08115.1"
                     /translation="MTINIKTEEHIMLIEIDRPQKYNAMTREMYTQLARAYYRLDRDD
                     DLWVGVVYANGPHFTSGLELTDWSDVFGSGAGFPLSGENEIDPFYMMNEERCRKPLIY
                     AVQGYCFTWGPEVMLNADIRVAASDTKFAMLEVRRGFFPCGGATIRLARDIGWSNAMR
                     YLLTGDQWSAEEAYRMGLVQYVTETGKQLEKAMELARKVASAAPLGVRNILQNARLAE
                     REGEQAAKKVIYQEVSKVMKSEDMKEGLQSFIERRAAVFKGK"
     gene            29298..29870
                     /locus_tag="PLF77_04330"
     CDS             29298..29870
                     /locus_tag="PLF77_04330"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPC08116.1"
                     /translation="MDPTFFNAGVFKFVILPLLIFSTRICDVTLDTLRIIYVSRGLKL
                     LAASIGFIEVLIWLFAITQIFKNLTNPIYYVAYAGGFAMGNYVGILIEEKMAVGTVVI
                     RTITQKDAGVLIKWLKAEGYGVTYIDAQGAFGPVKVLFTIVKRKDIDHVLGMIRTCNP
                     LAFYTIEDIRSSSKGVFPIVKSRKNRYKEP"
     gene            29946..30149
                     /locus_tag="PLF77_04335"
     CDS             29946..30149
                     /locus_tag="PLF77_04335"
                     /inference="COORDINATES: protein motif:HMM:NF039796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6485 family protein"
                     /protein_id="HPC08117.1"
                     /translation="MECKKEKNLDNCNCTYEPCSRKGACCDCLSYHLKQRQLPACCFP
                     KDAEKTYNRSFEYFTELVSQRRI"
     gene            complement(30277..>30674)
                     /locus_tag="PLF77_04340"
     CDS             complement(30277..>30674)
                     /locus_tag="PLF77_04340"
                     /inference="COORDINATES: protein motif:HMM:NF013895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPC08118.1"
                     /translation="LTLVLGLGESVSMQTMAITVHSMHHSKAESGWYFQTMQRELTRT
                     FLMALACAFIVGFIVVVWKNDFTAGLIISSGILTSLLFACFLGVSLPYSLHKLKLDVH
                     VASGPLTLALADICTVIVYFSLAAFILSS"
     assembly_gap    30675..30774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<30775..31427)
                     /locus_tag="PLF77_04345"
     CDS             complement(<30775..31427)
                     /locus_tag="PLF77_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPC08119.1"
                     /translation="MDHSIKIEDLNDPVMKHASTDFVSIYSDNTVGEALDCIRNCQTQ
                     STILYFYVLDGKARLVGTIQTRRLLTSPLDVKIEEIMSKNLVVIPASATLVEALEFFI
                     FHRYLAFPITDDHEKMLGVIDINVFTEEMIDIEEQGQVHSVFDTLGIKISEVRHKSPW
                     SVFRYRFPWLLATITSGTVCALLVGFFEATLAQSLVLAFFLTLVLGLGESVSMQTMA"
     gene            complement(31784..>32760)
                     /locus_tag="PLF77_04350"
     CDS             complement(31784..>32760)
                     /locus_tag="PLF77_04350"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HPC08120.1"
                     /translation="LAGLAVGRSEFAARAGIDALPMRDAFAVLFFVSVGMLFDPLSLI
                     QVPFVIATVLLVVVIIKPLAAALTVRMLGQPLAAAIPIGAAFSQVGEFSFILGTVALQ
                     LKIINDAGWNALVAASIISIALNPYVYRWARRLSSKTNKALPVVKSQPQTVNPSHCIL
                     IGYGPIGQIVHNLLTERGNEITVIDLNLKSVRQLNAQGYKAIYGDVLRPGTLEDAGIA
                     TAGSLIISTDIEDASEIIKNARLLNPDLQIMVRCAHLRDAAALRQAGATVVAAGEAEV
                     GVALSEVVTAADQMACVMAAEHRETVRRILYDKAFQQNETVSKKTRKK"
BASE COUNT         8801 a         7264 c         8299 g         8296 t
ORIGIN      
        1 ccatcgttga aatcgataac gccatgaagt ggggctacaa tcatcaactc ggtccttttg
       61 aatcctggga tgctgtaggc gtcaaggaag ccgttgccgt catgaagaaa ctgggcaaga
      121 aagttccccc caagattgaa gagatgctga agaataaatg cacctctttc tacaaagtgg
      181 ataaaaaagg cgtaaaatct tactatgatt tcaaaaagaa gggctatgtg aaactggacg
      241 aaaatcccaa gatcatcctg ctgcccggtc tcaaggaaag aaagaagacg attaaagaaa
      301 atcccagcgc ttctctggtc gatatcggcg atggtgtggc ctgcctggaa ttccatacca
      361 agatgaattc catcgatccc gatatgatga agatgatttc agaaggttgc gatatcgtga
      421 ataaagattt cacgggactg gttgttgcca accatgatca gcgcatgttc tccgccggcg
      481 ccaatctctt cctggtgttg gtggcgattc aggaaggcaa gtgggatgaa ctgaaaacca
      541 tggtcaggga attgcagatc gtcaatatga agatgaaata tctgcccaag ccggttgtca
      601 ttgctccggc aggtatggcg cttgccggcg gttgtgaaat cgccatgcac ggcgactact
      661 gtctggccaa cggcgagacc tacatgggtc ttgttgaggt gggtgtcggt gttattcccg
      721 cgggcggcgg ctgtaaagaa ctggccattc gcgcgacgga aggcattccc gccggtgttg
      781 tggaaagcgg attgaatatg cagtatcatt tcggcaagat ttttgaaaat gtcgccaccg
      841 ccaaggttgc gaccagcgct caggaagctc aggatctggg ctatatccgc aaaaccaaca
      901 tgatcaatat gagcagagac atgcagattt acgaagccaa acagattgtg ctgggcctgt
      961 ccaagttcta caaaccgccg aagcccgctt taatccccgt gatgggagaa aatctgcgag
     1021 gtttggccgg tgcgattctc agcaacatga aggccggaag atacgcttcc gaatatgatg
     1081 ttcatattgc catgaaaatt gcccatgtct tgggcggcgg tacctgcgcg gaaggaacct
     1141 ggatcacaga gcaggaaatg ctggatctgg aaattgaagc gtttatgagc ctttgcggtg
     1201 aacagaagac gcaggataga atatccgcca tgttgacgac cggcaagccc ctgcgcaact
     1261 aggatgaatc acgtgccgga ggtctgaaaa ccaagcctcc ggcctttaaa ataacaaaag
     1321 cctgtaagga ggaaaataat atgagtgaag ctgtcattgt cgcatccgta aggagtgccg
     1381 gtggaagata taaaagaggc ggtctgtctg aaaccagagc ggacgtcttt ggtgttcagg
     1441 ttctgaaagg gttgttggca aaagttcccc agttagaccc taaagatatt gatgatgtta
     1501 ttgtcggctg cgcttttccg gaagcggaag ccggaatgaa cttcggtaaa gtcctggctg
     1561 taggcgccgg attgcctgag ccggtatccg gtatggttct caaccgtttc tgctcatccg
     1621 gcgttcagtc cattgctgat gccacggcga agattcgcgc cggctggtcc gatgttatca
     1681 tcgccggtgg tgttgagtcc atgacccaca tccccatggg cggcggcatc ttccgtccca
     1741 accccgattg ggagtgggac accatgccca atgtgtacat gaacatgggc aacacggcgg
     1801 aaaacgttgc aatgaatcat ggcgtttccc gtgaagatat ggataaattt ggtttagaga
     1861 gcaatcgcag ggcttatgaa gccatcaagg ctggtaagtt taaagatgag attatcccca
     1921 tcaaagcctt caaatataaa aaagacaagg aaggaaaacg tatccgcacg gaatttgtgt
     1981 ttgacacgga tgatggtgtg agatggccct ccgacctggg ggccatgggg aaaatgaaat
     2041 cacctttcaa acagggtgga aactgcaccg ccgccaactc ttcgcagatg actgacggcg
     2101 ccgcctttac gctcctgatg actccggaaa aagccaaagc catcggcgta aaacccattg
     2161 ccaaactggc ttactatgcg gtagccggat gcaaaccgga agaaatgggc gtcggtccga
     2221 gatacgctat tcccaaggtt ctcaaacttg ccggtttgac aacaaaggat attgacgtct
     2281 tcgaaatcaa cgaggcgttt gcttcccagg ctctttattg catcagagag ttgggcattg
     2341 aagatcgttt gaatgccggt gacatcaatc ccaacggcgg tgcaattgct ctgggccatc
     2401 cgctgggatg cacaggcgcc aaactgaccg ctcaattgct gcatgaaatg aaacgacgcg
     2461 gcagcaaacg cggtatcgta tccatgtgta tcggcggcgg tatgggagct gctgcaatct
     2521 atgagatgct gtaaaaatgg ccccttccta gcttttgtaa aaaagctata actcttcttt
     2581 cgatcccttc gtcatcccct ttaccgggga tggcggaggg gtcgacctct ttcaatcgca
     2641 tccttcgaat aaagcaaagc gtactgtgcg aaaatcgtta aaaccgtttt cttaaatccg
     2701 gatacttttt ccccttgaaa taagcaatac tttaagttat agcacacacc tcttgggcgg
     2761 ttaactcagc gggagagtgc taccttcaca cggtagaagt cactggttca atcccagtac
     2821 cgcctaccaa tgaaatcaag gggttgtggt ttaggctgcg cccccttttc ttttctgcat
     2881 catcattaaa tgccggtttt ggaaaagatc atctgatctt ttgagaatcc tcgtttgcgg
     2941 caatccggga tggttgcaaa aaaagttgtt attagtcaga agttgattga caaaacttcc
     3001 gctgtgatgc tataaaacaa cagcttaagg atcaagaggt cttaccgtag ttggtgatca
     3061 tttatcccta tctgaataac tattggtggt catttattcc tatctcaata attaaaagaa
     3121 caccgatata aagcaaggag gatgttttat ggctttaaga gatgcgtatt ttgttgatgg
     3181 agtgcgtaca tggttcggaa agtccagaca ggacggccat tactggacca cccgcgcaga
     3241 tgatttggtg acgaaagtca tgaaagaact tattagaaga aacccgaagg ttccatgggg
     3301 cgaagttgac gacaacatct ggggtgcgac aacgcaggtt ggggatcagg gtactacgat
     3361 gggaagggta acggttctca ccgcaggtct tccggtagag gttcccggat tttcggtgga
     3421 cagaatgtgc gcgggcggta tgacctgcca ggccatcggt tcatctttcg tcaagacggg
     3481 ctgcgccgac attattattg caggcggtgt ggagcacatg gctcaccatc ccatgggagc
     3541 gggcgcagac ccgaatccgc gggttgtcac cgagaagatg gtcgaggtca agtatttcaa
     3601 tatgggcaac acggcggaaa aactgcacga ctggcttccg gaaaacggcg aacagccggt
     3661 gacgaaagaa gaagccgatg aatatgccta ccattgtctg gaaaaatatt tcaaggcttt
     3721 ggatgcaggc tattaccata agcactgtgt tgatatgacg gtttttaccc ccaatggatg
     3781 gaaagttgcc aattatgatg aacaagccaa gcgcggaacg acgcttgaga gcatcaaaaa
     3841 tttccggaaa ttccccttca aggcggaagg ccatgtcacc gcggcaaacg cttcggggtt
     3901 gaatgacggc gcttgcgttt cactcatcat gtcaggcaag cgatgtaaag aactgggcat
     3961 tcagcccaag atgaaatttg tcggtgccgg tttcgttggc gtggatccgt ccattatggg
     4021 atggggtcct gttccttcca cggaaaaggt tctcaagaac ttcaatatga aatttgatga
     4081 tctcgatatc atcgaattga acgaagcatt cgctgtccag gctgtcggtt tcatgaagca
     4141 ctttggcatg aagctacctc aagacccacg ccttaaccca tacggcggaa ccattgccat
     4201 gggccatccg ctggccagct ccggtgttcg cctgagcatg caattggcgc aggacttcga
     4261 gcttaatccg aaggcgaagt acggactgac caccatgtgt gtcggtctcg gtatgggtgg
     4321 ttctatcatt tgggaaaacg tcgtagggaa aaaattatag gaggttaagg ctatggcaaa
     4381 gtatataaca gaatgtaaac tcaatttctt gcagtcagca aaggctggga aaatagcgat
     4441 tgttacaatg gacaacggcc agttctacaa tatccccaat acgtggggtt ttgaggcctt
     4501 tgaatttcta aacaaaatta ttgataccgt tgaaaaagac aaggatgtta aaggctggat
     4561 cttgactggc aagcctttca tcttcaatgt cggcgccgac attatgagtg tggatccgac
     4621 tgcaacaaaa gaggatcttc tcaaaatcgg tgagatggga catggtacgt tcaaaagaat
     4681 catgaatctc aagatcccga cactggccgc cattaatggc gcggcgatgg gcggcggcgt
     4741 cgaggtcggg ctgtatcaca attaccgcac gatctccaag agcccgggcg gatgcgatca
     4801 ttatgcgctg cccgagtgct tcctcggttt gattcccggt tggggcggaa ctcagctggt
     4861 aaccaaactt tgcggtccgg aagcagccat tgagctcatc atcaacaacc cgctcaatat
     4921 gaacaaaatg atcaattcta taaaagctgt cgagatgggt ctggcggata gactcattgc
     4981 tccggccgag ttcatggatg aatccattgc tttactggag agaatcatta ccggcgagga
     5041 gaaaattgag cgcaagaaag tggatccgaa gatgagtgat gagctctaca ataagacaaa
     5101 agaattcatt accggtaagg ttcattcagg cgccattgca ccttacaaag ctctcgagtt
     5161 gatcaagggt gcggcggaat ggtcttatga tgaagggttc cagaaagaga ctgaagttct
     5221 ggctgatatg attatgtccg atcagttcaa gtgcggtgtt tactctttcg atctgaccca
     5281 gcgccgcgcc aagaaacttc ccggcagacc gccgaaagaa gtcaaaggca acaagatcgc
     5341 caagatcggc gttatcggcg ccggcctgat ggcttcccag atggccctgt tattcgcaca
     5401 gcgttatcag tgccccgtta tcatgaaaga catcaaacag gaatttattg ataagggctt
     5461 ggcttactgc agggaacagc ttgccaagac cgcgaaaaaa ggcaaactgt ccgcagcgga
     5521 tgccaagtgg ataggcgaag atcttatttc cggcaccttg acctatgagc cgttcaagga
     5581 ttgtgatttc gttatcgaag cggttttcga agaaatgggc atcaaacaga aaattttcgg
     5641 cgaactggaa gcagcctgca agccggaatg cctgttccta accaacacat cctctctgga
     5701 tgttgctgaa atgggcaaaa atctgaaaga tccttcccgc gtggtcgggt tccatttctt
     5761 taatcccatc gccgtgctgc cgcttgtcga gatcatcaaa accgacaaga ccagcgatgt
     5821 cgttctggca accgcatttg acattgccaa gaagatcaag aagacaccgg ttctggtgaa
     5881 gaatgcccct gcattcctgg tcaacagagt tttactggcc tggatggacg gtatcttcca
     5941 gtgcatcgac gaaggcgccg atttcatgca ggtggacaac gcagtattcg atctgggctt
     6001 ccccatgtcg ccgttcacac tggctgcgct ggtgggccct gctatcgcac tgcatgttca
     6061 ggaaaccctc aacaagtctt tccccgaccg attcccggtc aatgccggac tcaagaacct
     6121 ggtggcgaag ggcaagaaac aaatggttac ctttacggac aaaggcatcg atgtcgatcc
     6181 cgagatcaaa gccggttggc cgcaggggaa caagaaattc accgacgacg agatcaaggc
     6241 gcgtgtgttg gagcgtgtag ccaaagaagt cgacttgatt cttgcggaag gtgtcgtggc
     6301 ggaaccgaaa gatgtggata ccggcgtcat catgggagcg ggctggccgt tcttcatggg
     6361 tggcattacc ccatacctgg atcagaaagg aatttcgaaa aaggttgcgg gaaaagattt
     6421 ccatccgaaa ggtttcttaa ccgtagagaa atagtagcac tctaatcaag aaatgaaatt
     6481 gccgggcgac ccttatggtt gcccggcttt tttatgtttt ttcatccgac gaaaaaagca
     6541 aagacaaaac attaaacatg tttttctcag gcttgttcat gaacagtcaa cttattttcc
     6601 ggaacccctt ttttaatcaa ttttatattt ttgacaatgt gctgttgaat atttcattga
     6661 cttacacatt caaattttct ataattttat tagaaatggt ggaaatttta aaaagtttgc
     6721 gacgatcatt ggctgattga taaaaacata tctgatattg cgttgcggag gtcatagtaa
     6781 tgccgcctgc ggaaatgagg catgaaagag ttgtagtgga aacggaccgc tatcgcgtgg
     6841 aaggagatat aacactgcct caggaagggt atcgcagccg cctttccgat tatttaaacc
     6901 gaagtgatca ggaatttatc atccttgtaa acgttgaatt gttatcgctt gacgggtccg
     6961 gccgcgattg gcgtgccccg gtgcttatgc ttgccagaca gcatatcagg ctggttgtcc
     7021 ccgtgggcgg acctaatgct taaggtgaga gaaatcgtga atggccgctc ttttgcagaa
     7081 cgttgaaaat cattcctttt aaattcatcc ggcattggaa cagtttatct gaaacccgta
     7141 caatgacatt tatcttccat aaaataataa ccgcagatgt tgtaaaaacg atcatggcag
     7201 gatcgtaaag aatattttca atgaataaaa aacaggtgaa ggagtttgta aaatgaacgt
     7261 caaagatttg tttgatttga agggcaagat ttccgtcgtg acaggcggcg gcagaggcat
     7321 cggtaaattc atagcgacgg ggcttgcgga agccggttca gatgtcgtca ttgcgtcgcg
     7381 caagatggaa aacctcgaaa aagaagtaaa ggaactggaa aatctgggta tccgggctct
     7441 ggcggtgaaa tgcgatttgg ccaaaaaaga agatatcggc aatctggtca aagccgccat
     7501 ggataaattc ggcacgatcg acatcctggt caataatgcc ggactgacat ggggagcgcc
     7561 gacgctggat tatccgctgg ataagtggga aaaaatattt gacgttaatg tcaagggcgt
     7621 gtggattctt tcccagcagg ttgcccggat tatgaaggaa aaaggcggcg gcaagatgat
     7681 caatgtgtct tccatctacg gttcgcgcgg ctccaatgaa gaagagcatc cggccgttgc
     7741 ttataatccg tccaaatttg ccgtggaagg cttgaccaaa acgctggccc ttaaactggc
     7801 ccgttacaag atttatgtga attgcattgc tccgggtttt ttcaaaacgg atatgatggg
     7861 ccatttattc aagccggaga tgaaacccat tctcgatttg acggtcggcg ctattccgct
     7921 caagatgtac ggggaagaag acgatgtaaa gggattggcc gtatttctgg catccaaggc
     7981 gtccaattac attaccggcg gtattttccc cgtggacggc ggattttccg cgcagtaaaa
     8041 agtccggatg ggtttgcgaa agaggcatga cgccgaagat ttcacgggct ttgaaaagtc
     8101 atgaggtaaa aaacatgaaa aaaataaaac attcgttttt ccttttgctg tctttgattg
     8161 tggtagcctg tgctcccgct tatacgctca atcattcttc accagtcaaa cccctcagtc
     8221 tgcccgctga cgccgccgct cattactggg ctcagaatga atggtggtat tacacaggac
     8281 atttgaaagc ggaggatgga agggaattcg gatatgaatt gaccttcttc aagcgaatta
     8341 ccagtgagga taaggttccg cattttttta ttcccatccc ggcgcactgg ctcaaagatc
     8401 ttggaatgat ggggcatttt gttattaccg acattaccca tcagaaattt atgacgcaag
     8461 aggtgaataa tttcttcggc ttgccgtcaa aagcggacga tacaaaatac gatctcatga
     8521 tcggcacctg gacggcccgt gaagaagaag ggaaacatct tctgtacgct gaaatggatg
     8581 acggccggat cgaactggaa ctgcagccgg taaaggtcgc cgccctgcat ggacgacagg
     8641 gcatcgtcga taaaggaggg ggcaattcca attattatta ttcattgacg aacatgaaaa
     8701 cgtccggcac tttaaccatc gggggcgaat cgcttgatgt gcatggaaaa tcatggatgg
     8761 atcatgaata cggaaccatg aagctcactt acccgcagag cggatgggat tggttcagta
     8821 ttcagctgga gaaccattac gaattaatga tctatgttat cagaagtgaa ggcgatctga
     8881 aaaacagcct ggtcggtgga acctttgttc tgccgaatgg aaaaaccatt cccgtcaaag
     8941 cgcgggatat cgaaatcagg aatctggact actggcacag tgaaaaaacc gactccgatt
     9001 atccgtcttc atgggaaatc attgtcaggc ctttcaagct aaaactgaaa tgtcagccgg
     9061 ttatggctga gcaggaagtc aatttacaac ccatgcccta ctgggaaggg gccatatccg
     9121 tcagcggaac gtttcatggc aaatccgtca aaggtcaagg gtatattgaa ctggtgggct
     9181 attccagaaa atatccaatc caatacatta attaaaatga ttccgccatg gaacgataaa
     9241 cgaaccttta tttttaccat gaaattattc ttccacataa ggcaataggc tttacaagcc
     9301 tattgcctta tgttattaga aaaacgatga aaaaaccatt aaggagaact gtatgtccgg
     9361 ccattccaaa tggagcacga ttaaaagaaa aaagggtgcg atagatgcca aacgcggcaa
     9421 aatatttact aaaattatca aggaaataac tctggcggca aggctgggcg gaggggatat
     9481 tgaaggcaac tcgaggttaa ggcaggctgt catgctggcc aaggaagaaa atatgcccaa
     9541 ggataatatt gagcgggcga tcaaaaaagg taccggcggc ggcgaaggcg cggcggccta
     9601 tgaagaagtc acctatgaag gttacggacc gggcggtgcg gcggttcttg tggaaatcat
     9661 gacggataat aaaaacagaa ctgttgcaga aatcagacat atcttttcga agcatggcgg
     9721 caatctcggt gaaaacggct gtgttgcgtg gatgttcgcc aagaagggga gtattgttat
     9781 cgacaagaaa gtgattgatg aagatgctct gatggaactg gttttagaag ccggggcgga
     9841 agatgtgaaa acggaggaca gcgagtacga agtgattacc gatcccgggt cctttgaagc
     9901 ggtcaagaag attctggatg acaaggggat taaacatctg gaagccagga tcggcatgat
     9961 tccgtctaat accgtaaaac tggaagccaa caaggcggag cagatgctga agatgatgga
    10021 gaaactggaa gataacgacg atgtccagaa cgtatattcc aatttcgata ttgatgaaga
    10081 ggtcatgaag aagttgagtt gagatccata aaaatcattt ttaaaaatat ggaatgaatg
    10141 acattgcgaa ttctggggat agatccgggc agtcacgtca ccggttacgg cgtcattgag
    10201 aaaaaaggaa attatcttaa gcacgttgtt catggtgaaa taaaaccgaa aagaaactcg
    10261 tctttgtcgg aaatcttggt atatatttat cagcagcttt cctctctgat tgcgcagaca
    10321 gttccccagg ctgttgcgct ggaaaatatt ttttacggga aaaatgtacg cagtttgatt
    10381 aaacaggcgc aggtgagggg cgtggtgatt tttgccggcg cggataagga tttgaatatt
    10441 tttgagtatt cgccgctgga agtcaaaaaa gcggttgtgg gctacggcag agcggagaag
    10501 cgacaggtac aggtgatggt taaggctatt ttaaatctgc cggaaacgcc tcccccggat
    10561 gcggcggacg ccctggcaac ggccatatgc catgccaatt ttttaaagga caaacccatc
    10621 tgatgattgc tttaatcagc ggaaccattg cctacaaggg catctctcag attattgtag
    10681 acgtgcgggg cgtcggttat cgggttttta ttcctctgac gactttttat gaactgccgg
    10741 aaaccggcca gaccatttcg ctgcatattc atacgaacgt caaggaggat gccattcatt
    10801 tgtacggttt tcacacgctt caggaaagag acctttttca actgatgatt tccgtcagcg
    10861 gcatcgggcc gaaggttgcc atgaatattc tttccggcat cgcggcgccg gaactgctca
    10921 gggccatctc cggaggcaat gtcagtaaac tggtgaatgt tccgggcgtc ggtaaaaaaa
    10981 tggccgagcg tcttgtcctg gagttgaagg aaaaggttct gaaaaaaatg gcaatcgaag
    11041 aggctcccga agatcaaacg cagcatcttg cggatgatct catgcaggaa gatgtccttt
    11101 ccgctttaat gaatctgggg tatcgaaaca atgttgcccg ggatgcgctc aacaaggttc
    11161 tgcgcacatc cgaagcaaaa ttgagtatgg ataaaattct gaaagagaca ttgaaaattc
    11221 tagccgggta aaaagacgta taaggaatca gcgatggaaa aatcgaaaaa aaattccctc
    11281 atcagtcctt catgcgcgga agaagaaaat gattttgaag tcaccttaag accgaccatg
    11341 ttaaaagatt atgtcggtca ggaaaaggtg aaaagcaatc tggccatatt catcgaagcc
    11401 tccagaaaac gccgggaggc ccttgaccat gtccttcttt acggtcctcc gggactcgga
    11461 aaaacgacgc tggcctatat tatcgccaga gaaatgaacg ttgatatcaa ggtcacatcg
    11521 ggaccggtta tcgaaagacc gggagatctg gcggcgattc tgacgaatat gcatgaaaat
    11581 gaagtgcttt ttattgatga gattcaccgt ctttcccacg tcgtggagga aattctgtat
    11641 ccagccatgg aagattttca catcgatatc ttgatcggac agggaccgtc tgcccgatcc
    11701 atgaaactgg atattccccg attcactctg gtgggggcaa cgacgagagc gggatcgctg
    11761 acctcgcccc tgcgggatcg tttcggcatg cattttcgtc tggaattcta ttcgccgaag
    11821 gaactggccg taatcattaa acggtcggcc tccattctgg gtgtgaaact gacggcttcc
    11881 ggcgccgagg aattggccgg acgtgcgcgt ggcacacccc gtatagccaa ccgcttgctc
    11941 aagcgcgttc gcgattatgc cgaagtgaag gcggacggaa ccatagacgg gaaaatagcg
    12001 caggaagcgc tgactgcttt tgaagtcgat cagaaagggt ttgatcagat ggacagaagg
    12061 ctgctgttga cgctgattga gaaattcaac ggcggtccgg tcggtatcga gagtcttgcc
    12121 gccgccattg gagaagaaag ggtcaccatt gaagatgtct atgaaccatt cctgattcag
    12181 gaaggttatc tgcaaaggac ggcacgcgga cggatcgcct gttccagggc ttatgaacat
    12241 ttcggcctga aggcttccgg acagactgaa attttttaag cggacaacgt atcgcaacgg
    12301 atgttgttgt aagatgaaaa tattaatgca tatttgctgc ggaccctgca cgatttatcc
    12361 tctgaaagaa ttaagaacac agggacacga agtcaccggt gttttttaca atcccaacat
    12421 tcacccgtat caggaataca aaaagcgtta tcagacctta agccaatacg cggatcaaat
    12481 atatttaaaa atcatctggc ccgaagatta cttgatggaa gaatttttaa agggcgtcgc
    12541 tttcaaagga aaagaaaggt gctcttactg tctgcaggat cgtctgacgt ttacagctca
    12601 gatggccttg caggaaaagt acgatgcgtt cacaacaaca ttgttataca gcaagtatca
    12661 gaaacatgat ttcatccatc aagccggaga taatctcagt caaaagatgg gagtatcttt
    12721 ttactatcag gacttccgcg tggggtggga ggaaggcgtt aagatatcca aagaactgga
    12781 aatgtaccgt cagccgtatt gcggttgcat ttacagtgag aaagaacggt attacaaaca
    12841 gaataaacat tcctgagatc aattgttttt tcataatcgc tgtgtcaaat ttgccacact
    12901 ttaatcgaaa taagttggtc ttgctgcgta ttgtctttaa atagttcaat aattacaatg
    12961 atttcttttt taataaatat catcaaatat ggcatgttct ttgcctttga taaatttgaa
    13021 gaaagattag atatcctggc ataaaattat gaacttacaa cgtacaatca aaaaagagat
    13081 aaattgtttc agtatcgggc tgcatacagg gcgaaaggtt aatatgaaga ttaagccggc
    13141 acctgcagat acggggattg tttttatccg caaggatctt cccgaggctg aaccgattta
    13201 tgcgcggtat gacaatgttt gcgacacgac cctcgctaca acgctggggt ccaacggtat
    13261 taccgtttcg acagtggagc atcttttgtc ggcattcagc ggcatgggtg ttgataacgc
    13321 ggtcattgaa ctggattcgt ttgaagtccc ggtgatggac ggaagtgcgc ttccgtttgt
    13381 gaatatgctc aaagaagtgg gaacgcatgt acaaaagaaa cataaaaaaa tgctggtggt
    13441 aaagaaatcc gtttgtgtga atgcgggaga tgcaaaagcg cagttactgc cctcggaaca
    13501 atttaagatc acctatgaaa tcgatttcaa acatcccgtc atcggggttc aatcctacag
    13561 catgactttt tctgacgagg agtatgaaaa aaacatttgt gcggctcaga ccttcggatt
    13621 tttaaaggac ctggagtttt tgcaggccag aggtctggca ttgggcggat ctctgaaaaa
    13681 tgccattgtg cttgatgatc aaaagatcat taataaagaa ggacaaagaa tccctgatgt
    13741 ttttgtgaaa cataaaatcc ttgatgccat cggcgatttg tttttgctgg ggatgccgat
    13801 tatcggtcat tttgtcgcgt ataaatcagg ccaccgtttg aacaacctct tacttcggga
    13861 actgatcagc agaagagatt gctgggaaat cgtcaccgat gtaaacaagg cagaaaatct
    13921 taaaagccat ccattcaaaa ttccttcatt tagaactctg gatgccgttc aggcgtaatc
    13981 caaattcatc ttgcttttta tggtcatgtt gcctagtatt gcacgttgat ccgccaaatg
    14041 agctgaaccg ttatttatcg gttacttgga tcggatcatc gatgctgttt aattttgcag
    14101 ccgcaacggg tcctgctgcg tgggctgatg aattggtatg aaaaaaataa aaaataaaat
    14161 cggtctggta ttgatttttc ttttcatggg cgcgatgttt ttcccgtcac agacgtgcgc
    14221 tctggaaccg aaaattaagg atcttctgat taccaatgat gcagaaaatg ttcttttata
    14281 tgcccgtctg ctgaacggtt tcaaaccgca aatggaactg gcgattttag caggcattcc
    14341 gacgacgttt accatgtgga ttgaagtgta ccaagagcga tcccccatgt gggacaaaaa
    14401 aatcatccgg aaggaaatta aacgatcgat caaatatgat aacctgaaaa aaaccttcag
    14461 cgtcgtcagt gaaaagaagg atccggacat tttttcggat atggaaagtg cgcaaaaagc
    14521 gatgtctgac tacaacggta tcgttgccgt tcccatgtcg tctttaaaaa aggggcaaag
    14581 ctattatacg ctggttaaaa taaaaatgga caaggttcgt ttgccgctgc acatggaata
    14641 tgtctttttc tttgtttcgc tgtgggactt tgaaacgccc tggtacaggc aaaactttac
    14701 ttattaacac ggatcagttt tgtcatgata tgaatgattt caaaaccgtt actgtgatct
    14761 atggaaaata aacctggcag aagcattatt gacgaaaaag aattaagtaa acgacgccgc
    14821 gaacgaatta ttatggtggt cgtcggcgtt ctggccatta ttgttaccct gatggccagt
    14881 cagctgttca acaaggaaaa cctgcctctt tcgacgaaca tttttgttta cggcctgacc
    14941 agtatcaatt tcattctcat tgttctgttg atgtttctta ttatcagaaa cattgtcaaa
    15001 ctgttttacg aacaccgccg cggggtcatc gggtcccgct taagaacaaa actggtagcc
    15061 gcattcgtcg gcctgtcgct tgttccaacc attctgcttt ttttgtttgc gatcaatttt
    15121 ctttcttaca gtattgattt ctggttcaac attaaaatcg gcgatgcatt aaaccgttcg
    15181 ctggaagttg ctcaggttta ttaccagcag atggaagagc atgccaagtt caacgcccgt
    15241 caaatcagtg cggatataac aaaaaaccgc ctttacgaac aagatcgtgt tgaatatctc
    15301 aaaagcttcg tcacgcaacg tcaaaaaaac tacaaagcgg ggatggtgga aacatttttc
    15361 gatttccagc aggaaaaact gacgtttgcc gatgcggagg atccggatat actgccggtg
    15421 gatcccacgc cgaaaatgat ggaaaacatt tatctgggca aggaagaatc aatcatccat
    15481 ccggcggaaa agggcgatgt gattgtcggc atcgtgccgg tattttccta tgcagtgccg
    15541 acggaagtca tcggccgggt gtcggtgagc tacatgatcc ccaaggggtt tgtcgataaa
    15601 ttaagaagca ttgccgatgc gtcggagcaa tatcgcaaaa tcgaactgct gaaaaaaccc
    15661 attaaactca attatatggt cacgctgtcg atcgtgacgc tggtgatcat atttttggcc
    15721 atctggttcg gtttgacatt gtcaaaaggc attaccggcc ctgtgcagga tctggtgcag
    15781 gcaaccaaca gaatcacgca aggagacctg gacacccgca ttgatattga cgcggatgat
    15841 gaaataggga cgctggtgaa atcattcaat caaatgacga aagatttgaa aaagagtaaa
    15901 accagccttg aagaagccaa tctgaatctg gaacaacggc gcaaatacat ggaaaccgtg
    15961 ttgcgcaatg tttccgccgg ggtgatttcc gtggataaaa cgggtatcat tacgacgatc
    16021 aaccgtgcgg cggaacacat gtttgacatc cggtcggaga aaattctgtt tcgcaattac
    16081 cgggaactga tgattcccga acacatatcg ctggtggatg aatttcttca ggaaatgaag
    16141 gaaaaaaatg aaagcgtgct ggaaaaacag ctggaaatca tgctgcaaga gaaagcgctg
    16201 accgttttgc tcacgatcac aaccattcag gatgaggaag ggaatgattc cggtatcgtc
    16261 gttgttttcg aggatttgac attgctgcaa aaagcggagc gcgctgcggc atggcgtgaa
    16321 gtggcccggc gcatggcgca tgaaattaaa aatcctctga cgccggttca actctccgca
    16381 cagcggcttc agcggaaata tgaagataaa ctcggggaag acggttccgt gttcaaagaa
    16441 tgcacgcaga ccattatcga tcaggtggag gtattgaaaa atctggtgaa tgaattctcg
    16501 cgttacgcga ggatgccggt tacctcgccg tctcccaatg atttgaatgc tgtggtctcc
    16561 gaagccgtct cgctgtttca ggatgcgcat aaagacatcg tctttgattt gaaaacagcc
    16621 gatggtttgc cgaaattcga tctggatgca gagcagatca accgagtcat gatcaatctt
    16681 ctggataacg ctgtcgcggc gatgaataag aaaaacggac acattgaaat cgccgtcagt
    16741 tatgatgaac aacatcgccg tgcttctgtt tccgtcgccg atgacggtcc gggagtacct
    16801 tccagctata aacggaaggt ctttgagcct tatttttcca ccaagaaatc gggaaccgga
    16861 ctggggttgg ctattgtcag ttcgatcatt tccgatcatc atggtcaggt gaatgtcagc
    16921 gataacagcc ccacgggaac gattgtctct ttccaactgc cggtttcaaa agcgtcgtga
    16981 gactcacgtc tgataaggcc cgggcagcgg aaacagtcac cggaagattg cggaacgtcg
    17041 cagcgcatgt acccgaaggc ttgcgttacg gttcattccc gtgatatata aaaaaaagtt
    17101 tacgcgtaga aagagtgagc atgaaggaaa ccattcttgt tgttgatgat gaagaaagca
    17161 tctgtcagtc cctgaaagca attctcagcg atgaggggta tcaggttctg gttgccggca
    17221 gcggtgaaga agccgtaaaa gtggtcgaag aggagatgcc gaggctcgtt cttctcgata
    17281 tctggcttcc ggggatggac ggtctggaca cgctgaaagc catcaagaaa atcgatgccc
    17341 agaccatggt cattatcatg tccggtcacg ggacaattga gacagccgtg aaagcgacaa
    17401 aactcggcgc tttcgatttt attgaaaagc ctctttcgct cgataaaatc atcatcctgg
    17461 tcaataatgc cttaagtctg atccgtctta aagaagaaaa cattttatta aaacaaaaag
    17521 taacccatca gtatgaactt accggaaaca gccctgtgat cgtggatctc aaggaaatga
    17581 taagcattgt tgcgccgacg aatgcctgga tcctgatcat gggtgaaaac ggaacaggta
    17641 aagaactggt ggcgcgttcg attcatcgtt tgagcctgag agcggacaag gatattgttg
    17701 aggtgaactg cgcggcgatt ccggaagaac tcatcgaaag cgaattattc gggcacgaaa
    17761 aaggcgcctt caccggggcg acggaaaaga aacgcgggaa atttgatctg gcgcacgaag
    17821 gcacgatctt tctggatgaa gttgccgaca tgagtttgaa ggcgcaggcc aaaattttac
    17881 gcattctgca ggagaaaaaa ttcgagcggg tcggcggcaa cagattgatc gacatggatg
    17941 tgcgcgttct ggccgcaacc aataaggatc tggaagagga aatgaaagca ggccgtttcc
    18001 gtcaggatct ttattaccgc ctgcacgtca ttccgctggt tgttccgccg ttacgcgaca
    18061 gaaaagaaga tattccggcg ctggctgaaa ggtttatcct ggattttacc cggaaggaag
    18121 gtatggaacc gaaaatactc agtgatgacg ccatcgaact gctgatgaaa catgactggc
    18181 cgggaaatgt ccgcgaattg aaaaatatta ttgaacgaat gattattctg accccctcca
    18241 acgacataac cgccaaggat attccgccgc tcaatacgaa agaggcaaag agcgtatttc
    18301 ccggtacacc ggttgccgcc gctgattctt tcagggacgc caaaatggat tttgaacgac
    18361 agtttattct gaaaaaactg gaagaaaacg atggaaacat ttccagaacc gcggaagcca
    18421 tcggtatgga acgcagcaac ctccataaaa agctcaggag cctcaatgtg gtcatcaagg
    18481 gggacgctta aacctgtttt tcgttgttga tctggttttt catttcggaa attgaaaact
    18541 tgttgaaggg tataaagata acgcccagca ccaccacaac gatcatggaa agaaaatggt
    18601 aaacgaccgc aaacgaaagc gcttccactt ttgccacgct gaagaggctt aaccccagga
    18661 cacaggcaaa gtgccagttg ccgatgaaac cgggcgccgt cggaatggct atccccacaa
    18721 tcagaataat cattaaaaca aacgaagcga ttaccggaag atgaaatccg aaagccagaa
    18781 aaagtacata aatggccagc acgtccagca gccagaccgt gaccgaaaga aagatcagat
    18841 ataaaagcag gttgatattc gtgacaattt gcaacccgtt ggtgaagttg tacagcaact
    18901 cgtcgatttt atcggccagc cggcccggaa gcatcgccag tactttgttg accagttgaa
    18961 gggtttcttt cctgcgcagg atcataaaaa gaataaaaca gaatatcgcc agcgccagca
    19021 aaaagaaaat aatgctggaa tggatcatcc atgaaggcaa atcgatgaaa aagagaagaa
    19081 ggatggcaat gcccaggacg gtaaagctgt ccagaactct ttcaatgata atggtaccca
    19141 gcgcggacga catttttaca gaactttttt gggaaattaa atatggtctt gccagttccc
    19201 ccatccgtgc cgggatggcc gcaatcgcca gaaagccgac cgatgtgacc gagaaaaggg
    19261 agacctggtc gattttctcc aggggttgca gaataacgcc ccagcgatag gaccgcagat
    19321 actgcattaa aatgatcaga aaaagaaaga ggataacgta ggtgagttca attttattca
    19381 gatcattcaa aacatcctgc agactgattc ctctgacgga caagtagacc agaagcgcac
    19441 tgatcagaat gccaaagccg acttttttgt tcatgaattt acccggccat tatgctcaca
    19501 agccatcatt ctttgaccag gttgtccgca attctgtcgt gtatcagttg agaagaaagc
    19561 ttgtttccaa taacgccgac gcgaatcgcc accgtggtta ggttctttgc cttgtacgca
    19621 atatcgaatt tgactttttc atcgttgatg atcgtctgaa tggttccccg ggcgatttct
    19681 ttttccggtg ccacgtcaat gccgtgcatt ccggcgattg ttttttcaca ggccttccat
    19741 acctgatcga acgatttatt gtaatccgtt ttgagttctc cgttgatgta taaataggtg
    19801 ccggctcccg caccggcggc cgctcctccg accatcagcg gtacgcatcc ggaaaaaagg
    19861 aaagggatca atatgcatga gataaaggat agaaaaacat ttcttttcat gtgtcttcct
    19921 cccgtgattc gttggcggta tttaaagcac atgtcgtcgg tgattgcaac ggcaattatg
    19981 gggaaacagg gggtgccgca gaaaagtcag cgagtgggtt gattcagttt ttcaaactcg
    20041 attaaaagtt cttcctgctt tttactcagc gttgtgggaa tctccacaca cacttcgacg
    20101 atctgatcgc cgcgtccgaa cccgcggatg tgcgggatgc ctttgcccct gagcctgaag
    20161 gttttgccgt tttgtgttcc tctgggaatt ttcatttttt ccttgccctg cagcgtgggc
    20221 acttcgatgg tgcccccgag cgccgcctgc acaaaggaga tcggcgcccg gcaaatgacg
    20281 tcatctcccg acctgttgaa aaactcgtgc tcttccacgt tgatgaaaac ataaagatcg
    20341 ccgtcgggac cgccttgact gcctgtttcc ccttctccgc gcagacgcag acgcgaaccg
    20401 gtttcaacac cggcgggaat tttcaattga acggttttga tgatccgatc ttttcccgta
    20461 ccccggcagt tttcgcaggg ggtcgatata accttgccat gcccgttgca gtgcggacag
    20521 gtggagctga tggtaaaaaa gccgctggac tgtatcacct ggcctcgtcc cctgcaggtg
    20581 cggcagattt ccggggagga cccgggcgcc gctcctgtgc cctgacaatg atggcatgtg
    20641 gtcagttttt ccagatcaat tgctgtggtg gttccgaaag cggcatccaa aaaagagatg
    20701 gtcagatcgt aacgcaaatc ggcgccggca gtggcgtacg aatgagaccg cccccgagca
    20761 ttgccgtaac cgaaaacgtc atcaaagata ttgctgaaat gagaaaaaat atcgtcgaac
    20821 ccgctgaagc ccctgaatcc ggtgctgttt aatccttcgt gaccgtagtg atcgtaaatt
    20881 tctctttttt ctctgtcgct taatacttcg taggcttcgg cggcttcttt aaatctttcc
    20941 tccgcctttt tattgccggg gtttctgtcc ggatggcact gcatcgctat tgcacggtag
    21001 ctttttttta tttcgtcctc ggaagccgtt ctcgggacat tcagtatgtc gtaataatcc
    21061 ttcttcgtca tgcgcttttt aagtcaacct cgttttgtat tattttttta agagcctcaa
    21121 tttctgtcct gttgtctgtt ttctgcatgg cctgaagagc acgggagaaa ttttattgtt
    21181 cgattggttt ttcgccgatt ctttcccggt ctgagggttt tacgtccatg tgagggcttg
    21241 gccatctgct gaaagagcag gacatctccg ttgccaaacg cgacatctct gattttatgc
    21301 ttgcgtgtaa tttaacaatc aattggcgtt cgtcaatatt cgaatatcac ccgaccaatc
    21361 gtttcagggc ctcctcgtat ttttttcttg tgttttctat gatttccgaa gggagttgcg
    21421 gcgcgggcgg cttctggttc cagttaatgg aaagcaggta atcccttaag aattgtttat
    21481 cgtaactttt ttgcgcatga ccttctttgt attcgtcggc cggccagaaa cgcgaagaat
    21541 cgggagtcag cagctcgtca atcaacatta agttcccgtc caggaggccg aattccattt
    21601 tcgtgtcggc aataatgatt cctgccttca gcgccatgga ggctgctctc tgatagatcg
    21661 ccagggaaat atttattacg ctggcggtca gttcggctcc gataatatct gccatttctt
    21721 ttctgctgat cggcgaatca tgttctccct ccggcgcttt ggttgtcggc gtaaaaatcg
    21781 cttccggtaa tcgggaagat tctttcaacc cggccggtaa tgtgatgccg gagatagtcc
    21841 tgtctctctg ataatccttc cagcccgagc cgctgatata gccgcggaca atacattcca
    21901 ccggaagggg tgtggctttt tttaccagca tgcttcttcc ttccagactg tcgcggtagg
    21961 gagccgtctc tgccggaaac tcggcggggt tggtggagac aatgtgatgg gggatgatat
    22021 ctttcatttt ttcaaaccag aaagcggaca tctgattcag aatcacgccc ttgccgggga
    22081 cgggatccgg cataataacg tcaaaagcgg aaatgcgatc ggtggttaca atcagcagat
    22141 aattaccgac ggaataaata tcgcgcacct tgccgcgact gatcagattc agttcgtgga
    22201 agtctgtttt ctgtatgctt tttatcataa aaataaattc cttttagcgg aaaaatggat
    22261 tgtatctttt ttcatgtccg acggtggagg tcggtgtgct gccgtaatta tgtccgggca
    22321 tgactttggt atcatcgggt aaggtaaata ttttttccct gacggctctg gataattcct
    22381 gccacgagcc gcccggtaaa tccgttctgc cgacggcttc cacaaaaagc gtatctccgg
    22441 taaagacaaa gccgggcgtg taaagacaga ttcctccggg agaatgcccc ggtgtatgaa
    22501 tcaccctgag ttcgacgttg cccgcggaaa ggatgtctcc ttccttaacc agcctatcgg
    22561 ccgggggaga tgcttttgcg ccgaacattc tcaagatcat ggccggtgta tgcgtcagca
    22621 ttatggcgtc gtcctgatgg atgataattt tcgcgcccgt tgctttctgc atttccgtat
    22681 tgccgctgat gtgatccaca tggccgtgcg tattgacgat ataattaatc cgcaaattat
    22741 ttttttgagc ttccgccacg atgcctttaa tattatccgc cggatcgatg accagtgcgt
    22801 ctcccgttat cgggtcgcct acaatatagg caaataccgc catggaactg acctgcattt
    22861 gtttaacgaa catggcttgt ctccctggaa aaaagtgact ggctaataac attatttgac
    22921 gtaaaacgtc aatcgaattt tattctataa attgttgaat ttattggcgt atgagtaagg
    22981 ctccgaagaa tccatccatg ttgtgcctgt gcggaaatgt ccggaaaaat ccgctgctgt
    23041 ccgtcaacgg acggatgatg gtcgaagaag gcttacctaa agaaaattga ttatttaccc
    23101 ttaaaaaatg ctccacgata ttttcatttt cttccggaag gaccgagcag gtgcaatata
    23161 tcagccggcc tcctcgttta acggcagcgg aggcctgttg cagaatcttt ttttgttgtg
    23221 ccgcgaagga gaggatatcg ttttgcatta tgcgccattt gatttccgga ttgcggcgca
    23281 gcgttcccag tccggagcag ggtgcatcca ccagaacaca atcgaatcgt tccctgaagg
    23341 catccggcag accggcgctt acattgccgg cctgcgtttc gatgatggtg attcccaatc
    23401 ggatgatttc ctttttcagc tcagtgattt tttcctggtt gcgatcaaac gcaagaatga
    23461 aacctttgtt ttcaagaagg gccgccagat gagaggattt gcctcccgcg cctgcgcatg
    23521 catcgagaat ggattcattt ctttcgggat tgaccaggca ggcaatcaac tgcgcccctt
    23581 catcctggat gcgcagtaat cctttgtcga aaaattctgt tttctggatc ggcttcacgg
    23641 cggtgcttaa aatgattcca ttcggggaat agggggtggt ttgggattcg aagccggccg
    23701 ccgcaagttt ttcctttaaa tcgttgcgcg atattttcaa cgtgttgacg cgcagcgtca
    23761 gcggcggcag tgtgttgtcg gcagagcaca gcgcctttgt ctcttgcaga ccgaatgttt
    23821 tgatccatgt ctgaatcatc cactccggat gcgaatgaaa ggcggccagg tattcgaccg
    23881 gatttttctt ctcggaagga aaggatagat tctgaccgcg gcgcagaaag tttcttaaaa
    23941 tagcgttcac cagcgaactt ttgtcgggat tgattatttt ggcaatttca accccttcat
    24001 tgaccacggc aaaatccggc aaccgctcgc tgaatctgag ctgatacaga cttacgcgca
    24061 aaacattttt gatgtcttct tgcatttttg caaagtcgcc gcgatacagt tttttcaata
    24121 accagtccag atgaccgcgg aaacgcagaa cgccgtaaac aagatgggtg agaagacgcc
    24181 cgtcgggggt tccggataaa ttgtatttgt caagacaatc gttgatcaat ggaccggcaa
    24241 aatcatgggc ggcatggacc tgatttaaaa tctcgacggc ctgatggcga atagttttct
    24301 tcatgattcg ggatggatac ccaccgtgct tgtattttcg ggatgtttcc cccggacgct
    24361 ctgataaaaa tcgcggatca tgctcatatc gtgctccgga tgcccggtcg ggtatatcag
    24421 cggtcccgtc ccgccggttt tgcgcgcata atcaataaat gccagctgga tgggaacctg
    24481 cgctttgagc gcgatataat aaaaccctga tttccaggca tccgttttgt ggcgggtgcc
    24541 ttccggcgct aaagcaagga tgcatttttc tttttctttc agcagggcgg ccatttgatc
    24601 gacgatatgg tgacgcgctg ctctctcaac cggaatggct cccgtccagc tcatcaaaaa
    24661 ggaaaaagga ccgcggaata attctttctt cgcaatatac cgggcgtcga ttttcagggc
    24721 aaatgccaga agaattccat aaaacaaatc ccaattggag gtatgcggcg cgacgatcag
    24781 gacgaacttt ggctcccggg ggattttccc ggatgttttc cagcccagca ttttcagcag
    24841 cagcagggag attccccgca gcaaagattt tacgaaaggc gtgttaaaca tggtatggtt
    24901 cataaaagcc ccaggccttc ttcataaaaa attttatttc agctcagcga gtttccaatc
    24961 ttcaggtgat agccgcgcag gaaatcagca atgggcattc ttttctttcc cgccagttga
    25021 acgtctttta cgatcacata gccgtcggcg gttgcgacag acaaactgtc ggcttcgatc
    25081 gcgcagatgg ttccgggagg ctgctgtacg ttttcatgac gggcgaccgc actgaaaatt
    25141 ttcagggaga gtccgtccag gaatgtgtag gcggcaggcg aagggctcag cccccgaatg
    25201 agattgacaa tgtccgatgc gttgccggtc cagagaattt ttcccgtttc tcttgtcaac
    25261 cgcggtgcga acgttacgcc ggtggtttcc tgtttttccc ggctggcggt tccgctgatc
    25321 acctggtcaa ttgtttgaat cagcagcgca gcgcccagtt tcgcgagacg atcgtgaagc
    25381 tcgccgaaat tttcttcgca gtccagttgt gattcttcct gcagaagaat atcgccggaa
    25441 tccattcctt cgtccatcag cataatcgtg accccggttt ttttttcgcc gttgatgatc
    25501 tgccagttga tcggcgccgc gccgcgatac cggggcagca gggaagggtg gatattgagg
    25561 caacccagcc gcggatattc tataaccgat ttgggcagaa tctgtccgaa cgccgcaacc
    25621 gtgatcatgt ccggatggag ttgctgcagc aactccataa aagacggatc ttttactttc
    25681 tccggttgat aaacgttcag accgaattcc tgagccagaa tcttgaccgg cgaggcaatt
    25741 tctttcagcc ctcttccttt cgggcggtcg ggctgggtga caacaccggc aatcggatag
    25801 cgctgtccat gcaggaggcg caaagccgga agagcgaatg cgggcgtgcc cataaaaaga
    25861 atgcgagggt tggtcatcgg gaaaatatcc tttatttttt taatgtaaac acatatacaa
    25921 aatcatcggg attgctcttc ctgtccgcac atggcgggat ggctcaattg caaatttcga
    25981 tatacgttgt tttcgaaatt ttagtctcat aatccctgca aggtgtcaag gattacgaat
    26041 tattaggggt aagattatca ttactcgcac taaacatacc ctcgcgaaat taccgcggga
    26101 tttccgtttg tttctggtgg cgacctttct gtttggcttt tcgcagagta ttgttgattc
    26161 cacattcaat aattttttga atgaaacgta tctgatcgat aatctgcagc ggggcatttt
    26221 ggaaattccc cgcgaattgc cgggacttct ggtcgttgcg gtttccgcga ttgtattttt
    26281 tctgtgttca cggagacagg cagcgctggc caatctcctg tgtgccttcg gcattctact
    26341 catcggattt tattcgccga acttttccgt tatgctgatc tggcttttca tcttcagtat
    26401 cggacagcat ttgtttctgc cgttgaacca gagcataggc atggaactgg cagtcaaggg
    26461 acaaaccggc aggaggctgg gacagttcag tgccgttgcc aatgtggcaa tgattatcgg
    26521 cagcttctcc atctttgtcg ggtttcaata ttttgatctg gacttcaaga catcatactt
    26581 tgcggctttt ctcggattca gttccgttgc ggttttgatc ttcatgatga agaaagacga
    26641 accggttccc gtgaggatga aattcaacct gcgcaaggaa tatacgcttt actactggct
    26701 gaccattttg tacggaacac gcaaacaatt gtttctgacc tttgcgccct gggtgctggt
    26761 aaccgtcttt catcagaaaa cgcaggtggt ggcaacgctg ctgaccgtcg gctgtgtgat
    26821 cggcatattt ttcaagccca tgctcggtaa ggcgatcgac aaactgggcg agcgactgat
    26881 tctcgcgtca gaggcggtta tactcgtttt tgtctgcatc ggttacggtt tttcgaaaag
    26941 tatttttacg gagaccacgg cgctctacgc agtgtttgtc tgttacattg tcgatcagat
    27001 gctggcggcg gtatcgatgg cgcgggcgac gtatctgcag aaaatagccg tcagaccgga
    27061 agatgtttcg caaacgctga ccatgggcgt cagtattgat catatctttt ccattggcat
    27121 agcggtgctc ggcggtttta tatggctgaa gtttggctat cagtatttat ttttgaccgg
    27181 tgcggtgatt gccgtgctga attttttttc cgccttgcag atcaaaacaa aaacggaaaa
    27241 actgacgacc ccttaatatc aaagtggtca agaacattct gaggagatca acacatgaag
    27301 aaaataaaag taactgtcaa tgatttaatg cagcagaatt atgtttatta tcttcaggag
    27361 ccggtcggga aaaactttca tccgcaattt acccctgatt taacgccagg aaaaatgctg
    27421 gaaatgggcg tattcggcgg aaaatatatg accgattgca aggatgagtt tccgaaacgc
    27481 tggtttactc gcgcgaagct ttgtcatgaa cgacatgacc ccgatctgaa ttatttcggt
    27541 gtaaccgctt cacagccttt atccgtctgg cgtgaaaagg gctggatcta tcatgaagat
    27601 ccccgtggct ggtttcaatg gtattgccgg tattatatgg gccggcgatg ttccgatgat
    27661 gaacggcaga ttaaaagatg gaaagcgatg aagagacaca tttctcaaat caggaaacat
    27721 tgtcaaaaag gcgatatcaa ctgccgtccc agacaaaggc aggcgctgct gaactgggct
    27781 tacgatagca gaaacattta gggatcgagt tgtaatcatt attttcacag actaaaacgc
    27841 acagaccatt tcttttatga gggatatatg acggaaacat ggttacgaat aaccaatccg
    27901 gacaatccgc cgaatgatgc tatgctgagt gcatggatag gcaataaagc gtacaagtac
    27961 ttgcagcagg ttacaaaact cattgtggcg ctatatcccg atgtctttgt gccggaatgg
    28021 atctatggcg ggaaaaagca cggctggtcg ctccgctata agaaaagcaa atcgttttgc
    28081 accttgattc cggagaagag tcgttttgcg ctgctcattg ttttcggtgc acaagaacgg
    28141 gcaaaccttg agaccgtcaa agacagactt tcgcaacaca cccgaaggga ctacgatcgg
    28201 gcggcaacgt atcacgacgg gaaatggctg cttctccccg tcgataatga tcaggtcgta
    28261 gaagatctga aaattcttct ggctgtaaaa cgcaagcctc tgacggcgga atgcataatc
    28321 aaataacctg ataaattatc ttgagacacg tggtataccc atgcatggct cctttgcaat
    28381 cgcccgaatt tcttccgttg acacaagtag tccctttccc tatactccta acattactat
    28441 cttaaaggag taccttccat gacgatcaat attaaaacag aagagcatat tatgctcatt
    28501 gaaatcgacc ggcctcaaaa atacaatgcc atgacgcgcg aaatgtacac gcagctcgct
    28561 cgggcctatt acagactgga tcgcgatgat gatttatggg tcggggtggt ttatgccaac
    28621 ggaccgcatt ttacctccgg tctggaactg accgactggt cggatgtctt tggcagcgga
    28681 gcgggtttcc cgttgagcgg agaaaatgaa attgatcctt tttatatgat gaatgaagaa
    28741 cgttgccgaa aaccgcttat ttatgcggtg caggggtatt gcttcacatg ggggccggaa
    28801 gtgatgctca acgcggatat tcgcgtggcg gcatccgata ccaagtttgc catgctggaa
    28861 gtgcgccgcg gatttttccc gtgcggtggc gcaacaatcc ggttagccag agatatcggc
    28921 tggtccaacg ccatgcgcta tcttttaacc ggcgaccagt ggagtgccga agaagcctat
    28981 cggatggggc tggttcagta tgtcaccgaa accggcaagc aactggaaaa ggcgatggag
    29041 cttgcgcgaa aagtagccag tgccgcaccc ttgggtgtgc gtaacatttt gcaaaacgcg
    29101 cgcctggcgg aaagagaagg agaacaggcg gcgaaaaagg ttatttatca ggaagtatcc
    29161 aaagtgatga agagcgaaga tatgaaggaa ggcctgcaat ccttcatcga acgccgcgca
    29221 gcggttttca aagggaaatg atccatccgt tcaatgatgt gatgcgataa cttctgaaga
    29281 aaatgcgagg aagataaatg gatccgactt tttttaatgc cggagtattc aaatttgtta
    29341 ttctgcccct gctgattttt tccacgcgta tctgcgatgt gacgctcgat acgttacgca
    29401 ttatttatgt gtcgcgtgga ctaaaattat tggcagcgtc catcggattt attgaagttc
    29461 tgatctggtt gtttgctatt actcaaattt tcaagaatct gacaaatcct atttactatg
    29521 tcgcgtatgc gggcggtttt gcgatgggca attatgtcgg gattttaatt gaagaaaaaa
    29581 tggcagttgg cactgtcgtg atccgaacca ttacacagaa ggatgctggg gtattaatta
    29641 aatggctaaa ggcggaggga tatggcgtta catacataga cgcccagggc gcttttggtc
    29701 ctgtaaaagt tctttttaca attgtaaaaa gaaaagatat tgatcatgtt ctggggatga
    29761 tccgcacgtg taatccgttg gcattttata ctattgaaga cattcgttcc tccagcaagg
    29821 gagtattccc gattgttaaa tccaggaaga acagatacaa agaaccgtaa cagaagcgct
    29881 gagaataaaa gtgtttaatg tgaagaaata ttttcagaaa aagttagtcg tgaaggatag
    29941 agtcaatgga atgtaaaaaa gaaaaaaatc tcgataattg caattgcacc tatgagccct
    30001 gcagcagaaa gggggcctgt tgtgactgcc tgtcttatca cttgaagcag aggcaactgc
    30061 ccgcctgctg ctttccgaag gacgcggaaa aaacctacaa caggtctttt gaatatttta
    30121 ccgaactggt atcgcagcgc cggatataaa aagcatgatg ttcctgcaag ggaagcacgt
    30181 gaagtcttat cgggaggcag gtatttatga tgccgctgac gatgtttgtt gaaatcgccg
    30241 caattttgag tttcacaatc gtgatcttca ctggaattat gacgataaaa taaacgcagc
    30301 cagggaaaag taaacaatca ctgtgcagat atcggccagc gccagcgtca ggggtccgga
    30361 agcgacgtga acgtcaagtt ttagtttgtg caaggaataa gggaggctca cacccaaaaa
    30421 acatgcaaag agcaacgacg ttaaaattcc tgatgaaatt atcaggccgg ccgtaaaatc
    30481 gtttttccat acaacaacga taaatccgac aataaacgcg caggccaaag ccattaaaaa
    30541 cgtgcgcgtc agttcccttt gcattgtttg gaaataccag ccggattctg ctttactatg
    30601 atgcatggaa tgaaccgtta tggccatggt ctgcatggag acgctttcgc ccaggcctaa
    30661 aaccagcgtc agaannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatggcc
    30781 atggtctgca tggagacgct ttcgcccagg cctaaaacca gcgtcagaaa gaaagccaaa
    30841 accaggcttt gcgccagcgt ggcctcgaaa aaacccacca gcagcgcgca aacagtgccg
    30901 ctggtaatgg tagccaaaag ccaggggaag cgataacgga aaacgctcca gggcgacttg
    30961 tgtcgcactt cggaaatttt aataccaaga gtatcaaaca cgctgtgcac ctgtccctgc
    31021 tcctcgatat caatcatttc ctcggtaaac acgttgatat cgataacccc cagcattttt
    31081 tcatggtcat cagtgattgg aaacgccaga taacggtgaa aaataaaaaa ttccagcgct
    31141 tccactaagg ttgcgctggc ggggattaca acaagatttt ttgacatgat ttcttcaatt
    31201 tttacatcca acggagacgt caggagtctt cttgtctgga ttgttccgac caaacgggct
    31261 ttgccatcaa gaacataaaa ataaagaatg gtggattgtg tctggcagtt gcggatacaa
    31321 tccaatgcct cacccacggt attgtcgcta tagatagaca caaaatctgt gctggcgtgt
    31381 ttcatcacgg ggtcattgag atcttctatt tttatggaat gatccatgcg atgcctttcc
    31441 tgtatagaat cagaataacc tcaatgttga atatatacgg cttttttaac taacacaaac
    31501 catttcattt atcagatatt aatcatgatt aatacggctc atcagaacgc tgaagaatga
    31561 tatagctttt aagtataaaa ttaagaagcg gaaaagtccc tgtgacaatc ttctgcgcct
    31621 tatggaattg catgacaaca gtgacgctat cgtctgagat ggcgaaacgg ggttgacgat
    31681 gctctttccc gatggatgcg gaaaaaactt acaacaggtc ttttgaatat tttagccaac
    31741 cggtatcgca gcaccggatg tagaaaaaag ccgttttcaa atgttatttc tttcttgttt
    31801 ttttcgaaac cgtttcgttc tgttgaaatg ctttgtcata tagaatacgt cggacggttt
    31861 ctctgtgttc agcagccata acgcaggcca tctgatcggc tgccgtgacc acttctgaca
    31921 gggcaacgcc gacttcggct tcaccggccg caaccacggt agcgcctgct tgacgcagag
    31981 cggctgcgtc acggagatga gcgcaacgca ccatgatttg caaatcagga ttgagaagac
    32041 gcgcgttctt gatgatctcg gaagcgtctt caatgtcggt gctgataatc aggctgccgg
    32101 ctgttgcgat gcccgcatct tcgagcgttc ccggacgcag aacatcaccg tatatggcct
    32161 tgtatccctg ggcgttcagt tggcgaaccg atttcaggtt gaggtcgata accgtgatct
    32221 cgttgccgcg ttccgtcaga agattgtgaa cgatttgtcc gatgggaccg tagccgatga
    32281 ggatgcagtg actgggatta actgtttgcg gttgactttt gaccaccgga agggctttat
    32341 ttgtctttga agacaaccgg cgtgcccagc ggtagacata aggattcaac gcgatcgaga
    32401 tgatggaggc ggcgaccagc gcattccatc cggcatcgtt gatgattttg agttgaagag
    32461 cgactgtgcc gagaataaaa ctgaattctc caacctgcga aaaggcggct ccgatgggga
    32521 tagccgcagc cagaggctgg ccaagcatcc gaaccgtcaa agcggcggca agcggcttga
    32581 taataacgac cacaaggagc acagttgcga taacgaaagg cacctgtata agactcagcg
    32641 gatcgaacag catcccgacg gaaacgaaga agagcactgc aaaagcgtcg cgcatcggca
    32701 gggcgtcgat gcccgcccgc gcggcaaact cggaacggcc cacggccaga ccggcaagaa
//
LOCUS       DAOOLB010000020        31124 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_1240_length_31124_cov_46.049825, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000020 DAOOLB010000000
VERSION     DAOOLB010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 31124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31124
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1240_length_31124_cov_46.049825"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            629..2776
                     /locus_tag="PLF77_04355"
     CDS             629..2776
                     /locus_tag="PLF77_04355"
                     /inference="COORDINATES: protein motif:HMM:NF020119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HPC08121.1"
                     /translation="MGVADVKVDFNIAIPMTKAENPVGYNCNIYASAFYHTSPYNGTG
                     SIKRPTIFLATGYHRDLIGLMFAVIAFVPHDYNVVVLDMRGTGSGEGVWDPLSPIEQY
                     DVAYMIDKWIPAQPWSDGTVGMVGGSYMGIIQYLAAGLVEQVNGTPTHLKAIAPISAY
                     NDVWKDIVWHGGNFDLEFMAIWILMTDFISILPPDLIVGGVAEPNFNKTDFMNALSIW
                     AQHFKQLTVPLEWFMDSTKGTRNSWYDTKSPMIYWPDKPAGGWNFPDMPPAVGGGVIP
                     KNLPILTATGWYDIFTRGSFMNYEYGLKNHAPGNKAIIVGPWYHIDAAFMFPGVKGMG
                     IEGKEGIFTWDVLRRWMDWRLKGKDDPFLQEFPVLLYVLGENKWRAEKSWPLPSSRLS
                     NQTYYLSKAKSSLIFGDWFGVANYDDNYKLVPSVKTTDYNNVILGIKKPKVNPVLYHS
                     PTALNGAFSRSAQRWLGFSPLTIVSQLSRYTLDINLDSTLFWEDERSDETGVLTFSTE
                     PLASDMEISGPLKLTFWAKTKFTEPLKQAALDNFIDQIKSTFNIGEDKDMILQIADRK
                     DVQWVVEVNDVFPNGRAKNITSGWLSAAFRPYNAAKPTQVDPAYRAFDPFYFQPEKNP
                     NNIAEETVYQYVVELWPTTNVFKKGHRLRVSISASDFPHLFPVLRPSKNTIVIDEAHQ
                     AKLDFKVANKNGEGTLWKWIDGDVGDYLLTHKN"
     gene            3113..4849
                     /locus_tag="PLF77_04360"
     CDS             3113..4849
                     /locus_tag="PLF77_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HPC08122.1"
                     /translation="MNVQDNMSKESEKALFIQKSEEMKKLFVQLLLTSKNVSLYPAGH
                     SISLNSIGNFHETLEAYIRKHGDIRFEFERERVTCQEMNVQTGPPEEGSLPFTLFRDG
                     IRWLEFAEGITLEETRELLTIIHQYSVLTKEPEGDIVTAFWEAHFNHILYQADDFISE
                     QATDSLESMTKFQATPEDQGDGSESADVSETAALHEDAQEPESSEQIGLDPDSFVLTA
                     REQIALQDMIAREEMLSATEHLNMLLDMLLQYKEEKDFSIILEVISEEFDGSIRRHDF
                     EATLIILDGLRRVLDSGRLRSPYAHDLIEKLYGDISSADRCLEPLKDAWSSLNAQQIE
                     IFKNIFQHLQPQAIHTLTEFLLLGQPSQLEQLLQDTIIALINRDTNPLNQLIGKADEK
                     VAEKLVSVLSKVEGNISLKYLMKLARHPSPSARRLAVKTIGQIEGDYIPRIFEFLNDP
                     DESVRRTILKQMSRTRNEAAEDLLLQYLQNHQFDNEEAWYISECLVTLGRCGSERCIP
                     FLRDILLQRKRIAGAKKSVCRQGAAFALAALKMPEASRIIEAAGRSFSPSLRRIARKA
                     EKEVVQKNKKGS"
     gene            4852..6294
                     /locus_tag="PLF77_04365"
     CDS             4852..6294
                     /locus_tag="PLF77_04365"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPC08123.1"
                     /translation="MSEQKSISERWGHDFLRTLYHMTSTVRMYQDNNQIVRTSVESFH
                     KILHELTNDGDISLRLNRGRFHLGGEKIPYRRDAAVVVYNMVDFFSKRSIGSLTFLTS
                     SRDVPPEKIMTLARLLNEAVKHDRHVEWLEEQLTKNDFSWVQIQQLQDEDIIAEGENL
                     EEKKLEKARKNYFQAIEAVKEVANKVSHGTAGVRRIRRLAQNIVDLTKQDAALMIGLT
                     TLKDYDDYTYIHSVNVALLAACLGRHIELSDIVLEHLTICGLFHDLGKVDVPKEILFK
                     QGELTNGEWDTVKKHPVMGVKKILMLNANPSLRSKIILGPFEHHLNLDMTGYPQTLFI
                     NNVSLMGKIIHIADVYEAMTSARVYRPKSFMPDEVLKEMWKEAGTSFDTILLKRFIHM
                     MGTYPIGSVVEINNGSFGVVIDYPEETERSLPLIMLLEDDGKGTWQSGKMIYLADQNS
                     LEVKDRLSILRTISPEKFHIHPAEFFLRLK"
     gene            6375..7001
                     /locus_tag="PLF77_04370"
     CDS             6375..7001
                     /locus_tag="PLF77_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC08124.1"
                     /translation="MKKTVFLILLLLSLMACSKSSENAEDMMNEANLLWDGKQYTEPQ
                     KAVEFLSKAIKLQPDDADTYNQRGVAYKNLGNNQLALEDFNAAIRLQPDFVLAYYNRA
                     TVRNNLGQYQEAIADCGEAIRLKPDFAEAYNTRGINHNKLRSYKMAIEDFNKAIKLEP
                     RNANAYNNRGLAYFMLENNTQGCSDVKKACELGFCKALDLIKSRGYCL"
     gene            7108..9405
                     /locus_tag="PLF77_04375"
     CDS             7108..9405
                     /locus_tag="PLF77_04375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08125.1"
                     /translation="MISFNIFKSFKKPTTIKTLGVKNYQIYASLNRLSPGVDNIHLDV
                     HISSQVHNALKKTAFLLMVKHSGAEFFFKEYKKEFCESEKDALRRICNDVLLDGINKA
                     KKASEVQIDLLAQTALAKLFLEEIQLQYNILVEQIEPLVKMYQLSTKHDQNDVFMIKD
                     KLSEIKRHHHHITRLAGKELFELLADVNTRKLRDMRETHFPPRDILPGFFLANPMLHT
                     NNPSDDFFLIEEYVLMGQRSEDTDNYRSIKMILYDLFGQIDLTNEDGRNATEANERKF
                     QTVHGLVDAENCLYDALIMEPDNIGKMLDFYETEEQYEKAKNNKEQESILNKLKERKE
                     SQKNLLNLFYHQFKVAGLLPQIVANFEMKSVYEKYCPPLSPRQVREFLVNFWKRTSMR
                     KNLKRLKSLYGSQFNFGPLLQTVKHIKDCSTKEKKLYLILFLKQFSRYYRDLYNFYLI
                     QDAMNSINLVTEEKIILLSRENHSLFEFLLPDEKITEEKPIANHVIIKADIRGSITIN
                     HTMRKMGLNPASYFSLNFFDPISAVLAEYGASKVFIEGDAIILSLFENEDASEGNYSV
                     ARACGLAIRILRIVHSYNEKNKENNLPELELGIGICYSQDPPTFLFDGDSKIMISQAI
                     NQADRLSSCDKLLRKIFKSQNDIFNLFVFENEAEEIIEAAGEDATYRYNVNGIELNEE
                     GFHKLTREINLQTYIFPSETDENIKLYAGKVPLTNGNYQRIAIREAPIHKLQPSALDA
                     AGKITKKYYEVCTHPDIYDFTDKQY"
     gene            9552..11168
                     /locus_tag="PLF77_04380"
     CDS             9552..11168
                     /locus_tag="PLF77_04380"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HPC08126.1"
                     /translation="MINKNNSRYPTYRVLLIIAFLAGFVSLAYEIVATKVLYYFFNES
                     TITTSTVISIFLFGIGLGSFIFSRVEKKIPDQRKLILSAQLLIALYAVFIFTDYDLVP
                     SVFNFLYPLFGNDVIMLLFNKLFTSFLYLIFPTVLMGMVFPAIIVMSINAIEQLPQKI
                     GTIYSFDLFGAVLGALFSGFVFIPFFGIKALIFFSVSVNLIIGLLVFFQKNKKFLIML
                     AGFALMLLVFYFIIQPFKSDTAIYSSPDNKEKYIPGENLLFHTGKDTPYFQKVKIKEK
                     IFFSHSPFGELSVFDEVADTEQHRYLYIETRVQCSTVGLKQKEVSEINFANVSIDALD
                     NKNLRVLNIGLGCGFTLHEIVKHPNVKAVDVVEINPVMPEAAKCFSAFTDHVLENPKV
                     KIIFDDGYKYLLGTDKTYDLIIMDIEHPSVIYSSNLYTLEFYKLVKKALHKNGIFTQW
                     SYRPIPEAQVINFNTLKAVFPDVFPKISGVFNDLYYIAGKENIALDEREVTFLNKMLG
                     SNDKRLNTLNNPYFGTEMMLRDLFHVHQDI"
     gene            complement(11399..13837)
                     /locus_tag="PLF77_04385"
     CDS             complement(11399..13837)
                     /locus_tag="PLF77_04385"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPC08127.1"
                     /translation="MFKNKKSIWTFLLSTLMFLLMAGAAFAGEADIQLPDLTQVAFMG
                     GSLQGLTILNVGLIICLIGMAFGIMQYVQTKNLPAHKAMLDVSQTIWETCKTYLFQQG
                     KFLIALWILIAICIVYYFGALQGKGVSDIIIILICSILGILGSYTVAWFGIRINTVAN
                     SRAAFSSLSGNPLNIVNICLRSGMSVGLLLVSIELFFMIIILAYIPKELAGPSFIGFA
                     IGESLGASALRICGGIFTKIADIGSDLMKIVFHLPEDDPKNPGVIADCTGDNAGDSVG
                     PTADGFETYGVTGVALIAFLALALASNPEMGGKLIVWIFAMRILMILTSLASYFVNDV
                     ISKAAFGGKKEFNFEHPLTNLVWITSIVSIAVTFVASYYLLGDLTEPYTSLWWALAII
                     ISCGTIAGALIPEFTKVFTSTSSRHCEETVNASKQGGPSLNILSGLVAGNFSAFWEGL
                     TILILMFIAYMVSKNPAVMALMPPEFTFAAPVFAFGLVAFGFLGMGPVTIAVDSFGPV
                     SDNAQSIYELSLIESRPDAKDVVKKEYGIAPDFELAKHFLESADGAGNTFKATAKPVL
                     IGTAVVGATTMVFGIIILLEHLFGNVVAKLSLVQPEIILGLIMGGAVIYWFTGASIQA
                     VTTGAYQAVVFIKKNINLDKAEASIEDSKEVVKICTQYAQKGMINIFIVIFFMCLALA
                     FFNPYFFIGYLIGIAFFGLFQAIFMANAGGCWDNAKKIVEVDLKMKNTPLHEASVVGD
                     TVGDPFKDTSSVSLNPVIKFTTLFGLLATEIAVTMTNPNLKTGLAIFFFLIAIVFVYR
                     SFYSMRIASEKLGE"
     gene            complement(13856..14488)
                     /gene="pth"
                     /locus_tag="PLF77_04390"
     CDS             complement(13856..14488)
                     /gene="pth"
                     /locus_tag="PLF77_04390"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPC08128.1"
                     /translation="MLLSVLKKIFFRQVDEREQIMYLMIGLGNPGSRYARTRHNIGFM
                     VLEKIAAQWKISLTQKSFDALWNRGKINNVSVLLAMPQTYMNLSGKSVGRLMAYYKVD
                     IDHVIVIHDDLDLPFGTIRLKKGGGDAGHKGLKSVITALGSADFLRIRMGIGKPADKA
                     GVENYVLQKFNQEEQGVLPESIQLAAEAAADIVISGMQQAMAKYHTKNIK"
     gene            complement(14524..15210)
                     /locus_tag="PLF77_04395"
     CDS             complement(14524..15210)
                     /locus_tag="PLF77_04395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HPC08129.1"
                     /translation="MEVTDLAAQVRKEQKKGPSRRLRDKGFVPAVFYGRSAENILLAV
                     KSDELIKLHKDKKDHAFIKLIIDDEGSKKIEKLSLIKELQLQPLTGKLYHVDFYEVDE
                     KRKITMDVALNFIGKAIGVENGGELQHLKREVKVSCLPLDLPDHIDVDVTDLEIGDSV
                     KIRDLKVAEGVTLLDRPDTSVASVAMIKVAKVEEAAKEEGATEGAPAEGEAAATAGAA
                     AAPAEKEKGK"
     gene            complement(15233..16174)
                     /locus_tag="PLF77_04400"
     CDS             complement(15233..16174)
                     /locus_tag="PLF77_04400"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPC08130.1"
                     /translation="MLERIRIFSGNANKELAEKICNTLGVSLGKANVTTFSDGETRVE
                     IDENVRGMDVFIIQSTCPPVNVTLMELLIMIDAMKRASVDRITAVVPYYGYARQDRKV
                     ASRAPITAKLVADLITAAGANRVLSMDLHAGQIQGFFNIPVDNLFAKPVLLDYMKKTY
                     MDNTVIVSPDAGGVERARSFGKRLGASLAIIDKRREGPNEAEAMNIIGDVKGKRVIIL
                     DDMIDTAGTVVQAASAMKKAGALEISVCCTHPVLSGPALDRIENSDIKEAIVTDTIPL
                     NDRAKHCKKIKVLSVAGILGEAVRRIYYNDSVSSLFI"
     gene            complement(16200..16274)
                     /locus_tag="PLF77_04405"
     tRNA            complement(16200..16274)
                     /locus_tag="PLF77_04405"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16240..16242),aa:Gln,seq:ttg)
     gene            complement(16333..17172)
                     /gene="ispE"
                     /locus_tag="PLF77_04410"
     CDS             complement(16333..17172)
                     /gene="ispE"
                     /locus_tag="PLF77_04410"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPC08131.1"
                     /translation="MHWIAPAKINLFLKILRRRADGYHDIFSLMQKISIYDELIFAPL
                     HRGIVLHCPGMNLPENDGNLIVRAAKAIFAYANYSGGIEITLIKKIPMTAGLGGGSSD
                     AATTLLALNKICALKLKKNELMAIGAKIGADVPFFVFGNTALATGIGDKLKHLRNLPP
                     LNLVLIKPPFDISTKMVYESLNLRLTMGKNNYSIPRFLKLGDIVQVLQNDLESVSLKI
                     YPELDEFKKMLLGHGALGALMSGSGPTIFGIFRNGKEAKKALECINKEIPGNYTVFFA
                     KSL"
     gene            complement(17174..17572)
                     /locus_tag="PLF77_04415"
     CDS             complement(17174..17572)
                     /locus_tag="PLF77_04415"
                     /inference="COORDINATES: protein motif:HMM:NF020473.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HPC08132.1"
                     /translation="MEDKKQGSGFVIKDKRHFGESAEEQKQEETKTAGAEEKQKGPEP
                     TKQGESDYPPVNFTNFILSLSTSAFFHFGDLAESEDAAPQKNLPAAKQTIDILDMLNE
                     KTKGNLDKNESGLLQGIIYELKMRYVKEKG"
     gene            complement(17606..20197)
                     /gene="clpB"
                     /locus_tag="PLF77_04420"
     CDS             complement(17606..20197)
                     /gene="clpB"
                     /locus_tag="PLF77_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPC08133.1"
                     /translation="MRFDKFTLKVQEALQEAQTLAGSMGHQSIEPEHLLVCFLRQEDG
                     IAGAIINKLNASSQKIEKELDGYLKKQPSVGGAGAGQVYLGGRLNKTFDKAMTEAAQL
                     KDEYVSAEHVLVCIAEEKEGQAGKILRQAGVTKDNIFKVLKDIRGNQRVTDPNPEGKY
                     QALERYARDFNALARKGSFDPVIGRDDEIRRIMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVNGDVPENLKNKRVIGLDIGALVAGAKYRGEFEERLKAVLKEVISAEGEII
                     LFIDEIHTMVGAGASEGSMDASNMLKPALARGELRCVGATTLNEYRKYIEKDAALERR
                     FQPVLVKEPTVEDTIAILRGLKERYEVHHGVRIKDSAIVAAATLSHRYITDRFLPDKA
                     VDLIDESASRLRIELDSMPSEIDAIDRRIIQLKIELQSLKKESDKTAKERRDKIEKEI
                     ADLQEKMDQMKVHWSGEKDIIKKIQNIKSRIESLKTEEANAQRDGNLEKAAEIRYGKL
                     VALDKELQNEQKKLEDIQKNNQMLKEEVDAEDVAEVVSNWTGIPLSRMMESDVQKLLH
                     MEERLKLRVIGQEEGINAVSAALRRARSGLQDPNRPIGSFIFLGPTGVGKTELARALA
                     EFMFDNEQAMIRIDMSEYMEKHAVARLIGAPPGYVGYDEGGYLTEAVRRKPYSVLLFD
                     EIEKAHPDVFNILLQILDDGRLTDGHGRTVDFKNTVVIMTSNVGSQWIQDPSLDDEEK
                     RNRSMDALRATFKPEFLNRIDDIIMFSALKLEDIYKIVDIQMGLIDKRLKERKMSVEL
                     TEKAKNYIAGQGYSPVYGARPLKRSLQKLILDPLAMEILAGKFAEGDKILIDQSKKGE
                     ITLTKKS"
     gene            complement(20549..21421)
                     /locus_tag="PLF77_04425"
     CDS             complement(20549..21421)
                     /locus_tag="PLF77_04425"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HPC08134.1"
                     /translation="MKTINKTMIDHAVKIAREIKANALLVCADVVSDVSLIMDEIEKD
                     IAFIMVSREDESLSEEMKKDAKVLYIPNVNLTRVGIIKIAIAKGIILGALKRGDKVVC
                     LSGVPKFGYADSIFVIDVGKEFEILTSDFINDVLENVRPEVFNAVLNIALELAAQGRE
                     SRKVGTIFILGDDEKVMQLSRQMIINPFLGYPEDQLNILNPDLEETIKELSAIDGAFI
                     IKENGVLVTAGRHLSAALDSKDFPPGLGSRHIAAAGITSLTRAVAIVISESSGNVTIF
                     KNGKLFVTIEKPVE"
     gene            21644..22489
                     /gene="rpoH"
                     /locus_tag="PLF77_04430"
     CDS             21644..22489
                     /gene="rpoH"
                     /locus_tag="PLF77_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HPC08135.1"
                     /translation="MEFPVVTGTLNLYIAEINRFPLLTAEEEFKLAVRLKKYNDVSAA
                     EKLIVSNLRFVVKIAHEYRNYGFKLSDLIQEGNIGLIHAVKKFDPYKGYRLISYAVWW
                     IRAYIQNYLIRSWSIVKIGTTQAQRKLFFKLNQTKKELENLSRKNPEFSEIATSLGVK
                     DSEVEEMDLRMGARDVSLHEIINDESSNSHIDFLAYEGDDQEVSLIKKEEKSLVQRNI
                     AGALANLNERESYIILNRVMSDNPKTLQEIGEKYNISRERARQIEKQALKKMQLALPY
                     FKPNK"
     gene            22607..>31124
                     /locus_tag="PLF77_04435"
     CDS             22607..>31124
                     /locus_tag="PLF77_04435"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HPC08136.1"
                     /translation="MEKIIFFKYFHRLKSRLAGKHQNEKLTEDELLRSELFSAKQMRQ
                     HGKTIAGAHKLNEGLTSNQLLERLTENEKVLFQAREKLTKAVLENRRVTPAEEWFLDN
                     FYLIEEQIRTARRHLPKVYSRELPHLLNGPSANLPRVYDIALEMISHGDGRVDSKTLS
                     LFVSSYQSVTVLKIGELWAIPIMLRLALIENIRRIASRISKDRTNRDLADYWARKMIA
                     TVHKDPKNLFLEIADMVRSEPPIAGPFVAEMARQLQGQGPALALPLTWIEQQLSEYGQ
                     TIKNFVQMESQQQAADQVSMSNSIVSLRFLGTMNWREFVETMSFVDQVLREDPADIYV
                     KMDFATRDRYRHVIENIAKKSSLSETEVARMAILLARKTSNQKGGQDRTAHVGFYLID
                     NGLPELERLTKMRFSLFESVARFSRRFPLLIYVGSIFLLTFTAAFLLLAMAYADGLTG
                     WLLAVIWLVALLSTSHLSIALINWVSTLLITPAPLPRMDFSRGIPPEYRTLVAIPTML
                     LNDKNVGKLIESLEVRFLANRDENLRFALLTDFCDAKTEKMSDDGQLLRLVQKGIESL
                     NEKYADDRGDIFFLFHRSRRWNPKENIWMGYERKRGKLAELNAFLRGITHGPEGGLFS
                     LIVGDTQELRNVKYVITLDTDTKLPRDAAWQFVGTMAHPLNRAQYDEQKKRVVEGYGI
                     LQPRVSVSLPDISRSLYSIMHGSSSGIDPYTRAVSDVYQDVFGEGSFIGKGIYDIDAF
                     EASLNGRFPENYILSHDLIEGCYARSGLISDVQLYEDYPSAYKADVSRRHRWIRGDWQ
                     IARWIMSRVPSADGERLGNPLSVLSQWKIFDNLRRSLVPASLTLMLILGWLLSSSPGL
                     WTLAVLSIIVFPALVMSLMSVFQKQQNVILTHHLSSVFQKTGNNFLQVLFSFICLPYE
                     AYFNTDALLRTLWRMLISHKRLLEWTPSENTNNQRDDLAGYTRTMWFAPSLAAAVFAA
                     LLTAVPTGVPSAAFCLILWLLSPAVAWWFSQPSRLREARLSSAQINFLRKASRKTWAF
                     FEEFAGPEDNWLPPDNYQEHPVEVVAHRTSPTNIGLSLLANLTAYDSGYITVLKFIER
                     SSNTLRTMTSLERYKGHFYNWYDTQSLDPLLPLYVSSVDSGNLVGYLFILKSGLSSLP
                     DKKIIDPQILDGLKDTLGILNDSTGKSVSANLSQISKTLDDATASPPASLTEMKIVLE
                     KIISLAEETASCMRPESDASNAAGHWLKAFLQQATAALDEMLFLTPWLFDPQFSDLIR
                     EVPELDDIMTLRELTSLYEHVESKTEAMKRFESTAIDLSRISDLKNLIAESGNRAVNL
                     MQNIDLLARQCTEFTDIEYDFLFDKSCNLLSIGYNVTNSRRDTSFYDLLAAEARFSNF
                     VGIAQGKLPQESWFALGRLLTMAPGKKPVLVSWSGSMFEYLMPLLIMPTYEGSLLDQT
                     YKAAVAAHIEYGKKRSVPWGISESGYYAFDSHLNYQYRAFGVPGLGLKRGLAEDLVVA
                     PYASALALMVEPEKACLNLEKLASREFQGKFGFYEAIDFTPSRLTRGQSNAVIRSYMA
                     HHQGMTLLALTYLLLDRPMQKRFEAEPLFQSALLLLQEMVPKAVAFYASPSELISAQK
                     EPEISENPVRVFNTPDTTVPEIQLLSNGRYHIMLTNAGGGYSRWKNLDVTRFREDVTC
                     DSSGIFCYLSDVKTGDIWSTTYQPTLKKPRNYEAIFSDGRVEFRRRDYEFDVHTEIVV
                     SPEDDIELRRVTITNQSRLQKTIDITSYAEVVMTHSAADKLHPAFSNLFVQTEIIEQR
                     RAIVCTRRPRSEQEINPWMLHSMAVHGQDIEQISYETDRSRFIGRGNTLADPQAMGPP
                     SDLFGTLSDSQGSVLDPIVAIRSRITLGPEQSAVVDMVFGIGQTRDEAITLVDKYQDR
                     RIADRVFEMAWTQSQVLLRQINATEADAQLYSHIASSLIFPNDSLRAGAGVISENRRG
                     QSGLWGYAISGDLPIVLLRVSDQSHIELVRQLIQAHAYWRLKGLAVDLVIWNEEHAGY
                     RQLLHDQIMGLVSSGFVADLKDQPGGIFVRSTDQMPEEDRILIQTVAHVILSGNRGKL
                     ANQVKQRGGLKTAAPRMIPNRTHREPPVPKAAPPRSDLVFFNGLGGFDPSFREYVITS
                     SKSQITPAPWINVLANPTFGTVVSESGMSYTWADNAHEFRLTPWHNDPVSDRSGEAFY
                     IRDDERGHFWSPMPLPHRGLEPYTTRHGFGYSVFEHTERCIQSEVWIYTALDASVKFI
                     VLKIKNLTERTRRISITGYIEWVLGGLREKNAMHIITEADHQSGAIFARNSYNMEFPN
                     RICFFNSSASIRTITGNRMEFLGRNGTLADPAVMKRQYLSGKVGAGLDPCAAIQVPLE
                     MLEGEEKEVVFTLGMGRNVTEARKLAQFFSDPATAADALQAVQNYWREKLDAIQVQTP
                     DPSVNVLTNGWLLYQTLSCRLWARTGYYQSGGAFGFRDQLQDVMALIYTEPNFAREQL
                     LLAASRQFEEGDVQHWWHPPLGRGVRTQCSDDYLWLPLTTCCYISRTGDQGILDEIIP
                     FINGRPLNREEDSYYDLPVRSPEKANLYEHCLRAIKRGLRFGEHGLPLMGSGDWNDGM
                     NMVGIQGKGESVWLGFFLYDILTQFAKIASRRGDTSFEKCCRKEALQLRLNIEQHGWD
                     GLWYRRAFFDNGSMLGSARSEECRIDSIAQSWSVLSGAGNGHHSRLAMESLSQHLIRR
                     DIGIVQLLHPPFDKSGLNPGYIKGYVPGVRENGGQYTHGAVWAAMAFAALHDNNRAWE
                     ILTMINPVNHANSPESIAIYKAEPYVVAADVYAFSPHTGRGGWTWYTGAAGWMYRLIT
                     ESLLGLKKEDD"
BASE COUNT         8157 a         7783 c         7219 g         7965 t
ORIGIN      
        1 ctcgttgggc tcataaccca aaggtcagag gttcaaatcc tctccccgct accaagtaat
       61 tacaaggagt tagctgatgc agttagctct ttttttgcat tttggagggg ctttgagcta
      121 ccttctgcaa taatacattt cccccatgaa atatcttctt tgtgcgcctt ctttcaatgt
      181 tttcgttttg cacgctcatt tcttccgaca caactttcag gaatagtccc caaatatcac
      241 atctcgtttc cataagtata ttgacatcgc gggataaatc gtttagtgtt ccaatcactc
      301 atcaatatct taccaattga aaaaaagcac ggggggagga gtttgtaaaa tccactgcca
      361 aacaacattg ccatgaaagg gagggatcat gaaacaggtt gatttgctgc gtctagccaa
      421 atgttgtggt accattgtaa taggttgttt tttaaagggt ttgatttttg cagaaacgaa
      481 aaacagatgg ttccaatcgc ggcatcgttt caagatctgg tccatattcc tcgctgtgac
      541 agtggggatt cttataacct ccgctcatgt tcaagctaat acttcggatg ctgctgctca
      601 aacgcagata caggaagcgc tggcaaaaat gggggttgcg gatgtgaagg ttgatttcaa
      661 catcgcaatt cccatgacca aagctgaaaa tccggtcgga tacaactgca atatctacgc
      721 ctcggctttt tatcatacgt ctccttacaa tggcacggga agcatcaaac ggccgacgat
      781 ttttctcgca accggttacc accgggacct catcggtctg atgttcgccg tgatcgcctt
      841 tgtgccccat gactacaatg tcgttgtgtt ggatatgcgc ggaaccggtt ccggtgaagg
      901 cgtctgggat ccactcagcc ctattgaaca atatgatgtg gcctatatga ttgacaagtg
      961 gattcccgcg cagccgtggt cggacggtac ggtcggcatg gtcggaggct cctatatggg
     1021 catcatccaa tatcttgccg ccggtcttgt cgaacaggtc aacggcactc caacgcatct
     1081 taaagctatt gcgccgattt cggcctataa cgacgtctgg aaagacattg tctggcacgg
     1141 cgggaatttt gatctggaat tcatggccat ctggatcctt atgacggatt ttatcagtat
     1201 tcttccgccg gacctgattg tcggcggtgt ggcggagccg aacttcaaca aaacagattt
     1261 catgaacgca ttgagcatct gggcacagca tttcaaacaa ctgaccgtgc cgctggaatg
     1321 gtttatggat tcgaccaaag gaacgagaaa cagctggtat gacacaaaat cgccgatgat
     1381 ttactggccg gacaaaccgg ccggcggctg gaattttccg gatatgcccc cggccgtggg
     1441 cggcggcgtc atcccgaaaa atcttcccat cctaaccgca accggctggt acgatatctt
     1501 caccagaggt tctttcatga actatgaata tggattgaaa aatcatgctc ccggcaataa
     1561 ggccatcatt gtggggccct ggtatcatat tgatgcagcg tttatgtttc ccggcgtcaa
     1621 ggggatgggc atcgaaggga aggaaggtat tttcacgtgg gatgtcctcc gccgatggat
     1681 ggactggaga ctcaagggaa aagacgatcc gttcctgcag gagttcccgg tgctgctcta
     1741 cgtactggga gaaaacaaat ggcgtgctga aaaatcatgg cctttgcctt catccagact
     1801 cagcaaccaa acgtattatc tttccaaagc taaatcctct ctgattttcg gcgattggtt
     1861 tggagttgcc aattatgacg acaattataa gctggtcccc agcgtgaaga ccacagatta
     1921 taataacgtg attttgggga ttaaaaagcc gaaagtgaac cctgttctat atcatagccc
     1981 gacagcgctc aacggggcgt tctcccgctc ggctcaacgc tggctgggat tctcgcctct
     2041 gacgattgtg agccagttgt ccaggtacac actggatatc aatcttgaca gcacactatt
     2101 ctgggaagat gagcgcagtg atgaaaccgg tgttctgacc ttttcgactg aaccgctggc
     2161 cagtgatatg gaaatttccg gtccgttgaa gctgaccttc tgggcaaaaa caaagtttac
     2221 cgagccgctg aaacaggcgg ctctcgataa cttcattgac cagatcaaat ccacgttcaa
     2281 cattggagag gacaaggata tgatcttaca gattgccgat agaaaagatg tccagtgggt
     2341 ggtcgaggtc aatgatgtgt tccccaatgg tcgggcaaaa aatattacat ccggatggct
     2401 cagcgcggcg ttccgccctt ataatgcggc taaacctacg caggtggatc cggcatacag
     2461 agcatttgat cctttctatt ttcaaccgga aaagaatcca aacaatattg ctgaagaaac
     2521 ggtctatcag tatgtggtcg aattgtggcc gacaaccaat gttttcaaga aggggcaccg
     2581 cctccgcgtg agtatttccg cttccgattt cccccacttg tttcctgtgc ttcggccgtc
     2641 gaaaaacacc atcgttattg atgaggctca tcaggccaaa cttgatttca aagttgccaa
     2701 caagaatggg gaaggaacgt tgtggaaatg gattgacgga gacgtcggcg actatctttt
     2761 gacccataag aattaacagt atccaataat caaaaggaaa agggtggcgc gaaagcacca
     2821 cccttttttc ttgctcatca agccgtatta aagatattca agataagact ttaggctatg
     2881 aagattgtct tgacagaaaa aactaatttt tctatacttt ttacatacaa agcaattgcc
     2941 gttacactca aaaatcgcag tttttttgtg attccacaga ttaaagtgcc tgaaagaacg
     3001 cttcaggacc gagatcaagt accggctttg cttttgtccg ttattttgaa aactttatca
     3061 ggatggtaca agcatcatca ataaaaaatg attcatcgcc agcggtaccg aaatgaatgt
     3121 ccaagataac atgagcaaag aatccgaaaa agcccttttc attcaaaaat cggaagaaat
     3181 gaaaaaatta ttcgttcagc ttctgctgac gagcaagaac gtttctttat atccggcagg
     3241 tcacagtatc agtctgaatt ctatcggaaa ttttcatgaa acgctggaag cctacattcg
     3301 caagcacggc gatatccgat ttgaatttga aagggagcgc gtcacctgcc aggagatgaa
     3361 cgttcaaaca ggaccgcctg aagaaggatc cctgcctttt acgcttttcc gcgacgggat
     3421 acgatggctg gaattcgccg aagggataac gctggaggaa acccgcgaat tattaacgat
     3481 tattcaccaa tattccgtac tgaccaagga accggaaggg gatatcgtca ccgctttctg
     3541 ggaagcacat ttcaatcata tcctgtatca ggctgacgat tttatttcag aacaagcgac
     3601 ggattctttg gaaagcatga cgaaatttca ggcaaccccg gaagatcaag gagacggttc
     3661 tgaatccgcc gacgtatcag aaaccgcggc tttacacgaa gatgcacaag agcccgaatc
     3721 ttccgagcaa atcgggctgg atcccgattc ttttgtgctg acagccagag agcaaattgc
     3781 cctgcaggac atgattgccc gcgaagaaat gttgtccgcg acagaacatt tgaatatgtt
     3841 gctggacatg cttctgcagt ataaggagga gaaagatttc agtatcatcc tcgaagtcat
     3901 atcggaagaa tttgacggtt cgattcgccg tcatgatttt gaagcaaccc tgattattct
     3961 tgacggactc cgcagagttt tggatagcgg ccgtttgaga tccccttacg cgcacgactt
     4021 aatcgaaaaa ctttacggag atatttcatc ggctgaccgc tgcctggaac cgttgaagga
     4081 tgcctggagt tccttgaatg cacagcagat agaaatcttt aaaaacattt ttcagcatct
     4141 tcaaccgcag gcaatccata ccctgacaga atttctgctt ttagggcagc cgtcacagct
     4201 ggagcaactt ctgcaggata ctattatcgc cctcatcaat cgcgatacca atccgctgaa
     4261 ccagttgatc ggaaaggccg atgaaaaagt cgccgaaaag cttgtttccg tgctgtcgaa
     4321 agtggagggg aatatttccc tgaagtatct gatgaaactt gcgcgacatc cttcgccgtc
     4381 cgcccggcgg ctggccgtca aaaccatcgg gcaaatcgag ggggattaca tccccaggat
     4441 cttcgaattc ctcaacgatc cggatgaatc ggtccgccgg acaattttga agcaaatgag
     4501 cagaacaagg aacgaggcgg ctgaagatct cttactgcaa tatcttcaga accatcaatt
     4561 cgataacgaa gaggcctggt atatttcgga gtgcctggtt actctgggca ggtgcggatc
     4621 ggagcgttgc attccctttt tacgagatat cctgttgcaa cgaaagcgga tcgccggcgc
     4681 aaagaaatcg gtatgccgtc agggggcggc gtttgcgctg gcggctctaa aaatgccgga
     4741 agccagtcgg atcattgagg cagcgggacg cagtttcagc cccagcctgc gcagaatcgc
     4801 ccggaaagca gaaaaagaag ttgttcagaa aaacaaaaaa ggatcataat catgtcggaa
     4861 caaaaatcga tttcggaacg atggggacat gattttctga gaaccctgta tcatatgacc
     4921 agcacggttc gcatgtatca ggacaacaat cagattgtcc gtacaagcgt cgagtctttt
     4981 cataagatcc tccatgaatt gacaaacgac ggagatatca gcctccgtct taaccgaggc
     5041 cggtttcatc tgggcggaga aaaaattccc tatcgcagag atgcagcggt tgttgtttac
     5101 aatatggtgg atttcttctc aaagcggagc atcggaagcc tcaccttttt aacatcctca
     5161 cgcgatgtgc cgcccgaaaa aatcatgacc ctggcacgtc tgctcaatga ggccgtcaag
     5221 cacgatcgtc atgtggaatg gcttgaagaa caattgacga aaaatgattt ttcatgggtg
     5281 cagattcagc aacttcagga tgaagacatc atcgccgaag gggaaaacct ggaagaaaag
     5341 aaacttgaaa aagcgcgaaa aaattatttt caggcgatcg aggcggtcaa agaagtggcg
     5401 aataaagtat cccatggaac ggcgggagtc cgcagaatca ggcgtttggc acaaaacata
     5461 gtggatctga caaagcagga tgccgcgttg atgatcggcc tgacaacgct caaggattat
     5521 gatgattata cctacattca ttccgtaaac gtcgcgcttc tggccgcctg tctgggaaga
     5581 catatagaat tatcggatat cgtgctggaa catttgacga tttgcgggtt gtttcatgat
     5641 ttggggaaag tggatgttcc caaggaaatt ctctttaagc aaggtgagtt gaccaacggg
     5701 gaatgggaca cggtcaagaa acaccctgtc atgggggtca aaaaaattct catgttgaat
     5761 gccaatccgt ccttaagatc aaaaatcatt ctcgggcctt tcgagcatca cctgaatctg
     5821 gatatgaccg gttatcctca aacgcttttt atcaataatg taagccttat gggcaagatt
     5881 attcatatcg ccgatgttta tgaagccatg accagtgcga gggtttaccg gccaaaatct
     5941 ttcatgccgg atgaagtcct taaagagatg tggaaagaag cggggacaag cttcgatacg
     6001 attctgttga agcgctttat tcacatgatg ggaacatacc ctataggatc cgttgtagag
     6061 atcaacaacg gatccttcgg cgtggtgatt gattaccctg aagaaactga acgcagtctt
     6121 ccgcttatta tgcttcttga agatgacggg aaaggaacct ggcaaagcgg aaagatgatt
     6181 tatctggcgg atcaaaattc tctggaggtt aaagaccgat tgagtatcct ccgaacaatt
     6241 tcgccggaaa aatttcacat tcatccggcg gagttttttc tgcgtctaaa gtaacagaaa
     6301 tatttttatt atatttttcc gataattatg ctagtgaagt tgcatactaa acactattta
     6361 tggaggcaga tattatgaaa aaaacggttt tccttatcct tcttcttttg agcctgatgg
     6421 cctgctcaaa gagttctgaa aatgctgaag atatgatgaa tgaagcaaac ctcttgtggg
     6481 acggcaaaca atatacggaa ccgcaaaagg cagtcgaatt cctaagcaaa gccattaagc
     6541 tgcagcctga tgacgccgat acctacaacc agcggggagt cgcttataag aatctgggga
     6601 acaatcagct tgccctggaa gattttaatg cggccatccg cttgcagccg gattttgttc
     6661 tggcttatta taaccgcgca accgtccgaa acaatctggg ccagtatcaa gaagcaatcg
     6721 ccgactgcgg cgaagccatc cgtctcaaac cggattttgc agaagcatac aatacacggg
     6781 gcatcaatca taacaagctc agaagctaca aaatggcgat tgaggatttc aacaaggcca
     6841 ttaaactgga accccgtaat gccaatgcat acaacaatcg cggcctggct tattttatgc
     6901 tggaaaataa cacgcaggga tgcagcgacg tgaagaaggc ctgtgaactt ggattttgta
     6961 aggcgttgga tttgattaaa agtcgcggat actgtctcta ggccggcatt gatctgatcg
     7021 tgaaaattat tcatgcatcg tatccgtttc atgaattatt ggcggaccga atagtgattc
     7081 gattatccaa taagcataaa gggtgtcatg ataagtttta atattttcaa atcattcaaa
     7141 aaaccgacga cgattaagac gctgggtgtt aaaaactacc agatctatgc gtctctgaat
     7201 agactttctc ccggagtgga caacattcat ctggatgtcc atatcagctc ccaggtgcat
     7261 aacgccctta aaaagacagc ctttctcttg atggtaaaac acagcggggc tgaattcttt
     7321 ttcaaagaat ataaaaaaga gttttgtgaa tcggaaaaag acgccctgag acgaatctgc
     7381 aacgatgttt tgctggatgg aatcaataaa gcgaaaaaag cgtcggaagt gcagatcgat
     7441 ctgctggcgc agacggctct ggcaaaactt tttctggaag aaattcagtt acagtacaac
     7501 attctggtgg aacaaatcga acctctggtc aagatgtacc agttgtcgac caaacatgac
     7561 cagaatgatg tgttcatgat caaagataag ctgtcggaaa taaaacggca tcatcatcac
     7621 atcacccgcc tcgcgggaaa agagctcttt gagctcttag ccgatgtcaa cacgcggaag
     7681 ctcagagata tgcgggaaac gcattttcct ccgcgggaca ttttgcccgg cttttttctg
     7741 gcaaatccaa tgctgcacac gaataatccg agcgatgatt tttttctgat cgaagaatat
     7801 gtactcatgg ggcagcggtc cgaagatact gacaattaca ggagcatcaa aatgatcctg
     7861 tacgacctgt tcggacaaat cgatttaaca aacgaagacg gccgaaatgc gacagaagcc
     7921 aatgagcgaa aatttcagac ggttcacggc ctggtcgatg cggagaattg tttatatgat
     7981 gcgttgatca tggaaccgga taatatcggc aagatgctcg acttctatga gacagaggaa
     8041 caatatgaaa aggcaaaaaa caacaaagaa caggaaagca ttctcaataa gttaaaagaa
     8101 cgaaaagaga gccagaaaaa cctgttgaat cttttttatc atcaattcaa agttgccggc
     8161 ctgctgcctc aaattgtcgc caatttcgag atgaaatccg tctacgaaaa atattgtcct
     8221 ccgttgtctc cccggcaggt aagagaattt ctggttaatt tctggaaaag aacatccatg
     8281 agaaagaacc tgaaacgatt gaaatccctt tacggtagtc agttcaattt cggccctctc
     8341 ctacaaacag tcaaacacat caaagactgc tccacaaaag aaaaaaaatt gtatttgatt
     8401 ctgtttctta aacaattttc ccgatactac cgcgacctgt ataattttta tcttatccag
     8461 gatgcgatga attccattaa cctcgttacg gaggagaaaa ttattctgtt atcacgggaa
     8521 aaccactcgc tttttgagtt tctgctgccc gacgaaaaaa ttacggaaga aaaacccatc
     8581 gccaaccatg tcattattaa agcggacatc cgcggatcta ttacgatcaa tcataccatg
     8641 agaaaaatgg ggctgaatcc ggcctcctat ttcagcctga atttttttga tcccatatcg
     8701 gctgttcttg ccgaatacgg ggcctccaag gtctttattg aaggggacgc cattatcctg
     8761 tcccttttcg aaaatgagga cgcatcggaa ggaaattaca gtgtggccag agcgtgcggt
     8821 ctggccatca gaatcctgcg cattgttcat agttataatg aaaaaaacaa ggagaacaat
     8881 cttccggaac ttgaactggg catcggcatt tgttactctc aggatccgcc gacgttcctg
     8941 tttgatggcg attccaaaat catgatctca caggccatca atcaggcgga ccgcctgtcc
     9001 agctgtgaca aattactgcg aaaaatattt aaaagccaga atgatatttt caatttgttt
     9061 gtgtttgaaa atgaagcgga agaaatcatt gaagcggcgg gagaagacgc cacgtatcgc
     9121 tacaacgtca acgggattga actgaatgaa gagggcttcc ataaactcac ccgggaaatt
     9181 aaccttcaaa cctatatctt tccttccgaa accgatgaaa atatcaaatt gtatgcgggg
     9241 aaggtaccgt tgaccaacgg caattatcag cggattgcca tccgggaagc gcccattcac
     9301 aaattacagc ctagcgctct ggatgcagcc ggaaagataa caaaaaaata ctatgaggtc
     9361 tgcacccatc cggatattta cgattttacc gacaaacaat attaattgaa tttccttgat
     9421 ccggatgttt caatagcgta tcagataaaa ctggtttatc gttcccggga atttaagata
     9481 tccttttctg tccgattcca tggcctccgg aagatactga gattattgat cggagttttc
     9541 gtaaaaaagc aatgataaat aaaaacaaca gccgttaccc gacgtatcgc gtcctgctca
     9601 ttatcgcgtt tctggccggt tttgtcagtc tggcttatga aatagttgca acaaaagtac
     9661 tgtattattt tttcaacgaa agcaccatta cgacctccac tgtgatctcc atctttcttt
     9721 tcggtatcgg gctgggaagt tttattttca gccgggttga gaaaaaaatc cccgatcaac
     9781 gcaaattaat tctttcggct cagctgctga tcgctcttta cgccgttttt attttcacgg
     9841 attatgatct ggttccatcg gttttcaatt tcctttaccc gttgttcgga aacgatgtca
     9901 tcatgctgct gttcaacaaa ctgttcacaa gttttctcta tcttattttt cccacggttc
     9961 tgatgggcat ggtctttccg gccattatcg tcatgtctat caatgcgata gagcaattgc
    10021 cgcagaaaat aggcacgata tacagtttcg atctttttgg agcggtactg ggagcattgt
    10081 tcagcggttt tgttttcatc cccttcttcg gcatcaaagc cctgattttc ttttccgtct
    10141 cggtcaacct gatcatcggt ctgcttgttt tctttcagaa aaacaaaaag ttcctcatta
    10201 tgctcgccgg tttcgctttg atgttgcttg ttttctattt cattattcag ccgtttaaaa
    10261 gcgacacggc catttattcc agtcccgaca acaaagaaaa atacatcccc ggtgagaatc
    10321 ttcttttcca taccgggaaa gacaccccct attttcagaa agtaaaaatc aaagaaaaaa
    10381 tttttttttc tcattcgccg ttcggtgagc taagcgtgtt tgatgaagtt gcggacacgg
    10441 aacagcatag atatttatac atcgaaacac gcgttcagtg ctcgacggtg ggattaaaac
    10501 aaaaagaagt gagcgaaatt aattttgcca atgtctccat cgatgccctg gacaacaaaa
    10561 atcttcgggt tttaaatatc ggtctgggat gcggttttac gcttcacgaa atcgtcaagc
    10621 atccgaacgt gaaagctgtt gatgtggtag aaattaaccc ggtaatgccc gaagccgcaa
    10681 aatgcttcag cgcattcacg gatcatgttc tggaaaaccc caaagtaaaa atcatttttg
    10741 acgacggtta caagtatctt ctgggcaccg acaaaacata tgacctgatt atcatggata
    10801 tcgagcatcc gtccgttatt tattcaagca atctctacac gctggaattt tacaaactgg
    10861 tcaaaaaagc gctccataag aacgggatct ttacgcaatg gtcctacagg cccatacccg
    10921 aagcgcaggt cattaatttt aatactctta aggctgtatt cccggatgtt tttccgaaaa
    10981 tatccggcgt ctttaacgat ctttattata ttgccggaaa ggaaaatatt gctctggacg
    11041 aacgggaagt gacgtttcta aacaaaatgc tcggctccaa tgacaaacga ctcaacaccc
    11101 tgaacaatcc atactttggg acagaaatga tgttgaggga tctgtttcac gttcatcagg
    11161 atatctgaaa aacaatcaga tacgcaggag ggaaaagctg ccatcgcaat ccttttggtt
    11221 atgcagcttt aatcaacgcc tggccggatg gagaccggat accaaagcac atggatatga
    11281 tcggattgca aaagcacttc aaaaaacagt caataatatt atgaagcagc caaaatcgat
    11341 gtaaaaaagg cgcccctttt tcaaagggcg ccttttggtt taaagagttg agtacttctt
    11401 attcaccaag tttttcactg gcaattctca tgctatagaa tgaacgatac acgaaaacaa
    11461 tcgcgatcag gaagaagaaa atagccagac ccgtcttcag attcgggttg gtcatggtca
    11521 ccgcgatttc cgttgccagc aatccgaaca gggtcgtgaa cttgatgacc gggttcaaag
    11581 aaaccgaaga ggtatctttg aagggatcgc caacggtgtc gccgacaacg ctggcttcat
    11641 gcagcggggt gtttttcatc ttgaggtcga cttcaacaat cttcttggca ttgtcccagc
    11701 aaccgccggc attggccatg aagatcgcct ggaacagacc gaagaaagcg ataccgatca
    11761 gataaccgat gaagaagtag gggttaaaga aggccagcgc cagacacatg aagaagatca
    11821 cgatgaagat gttgatcata cccttctgag catactgggt gcagatcttg acaacttctt
    11881 tactgtcttc aatcgaagct tccgctttat cgagattgat gtttttctta atgaagacaa
    11941 cggcctgata ggcaccggtg gtaaccgcct gaatggaggc gccggtgaac cagtaaataa
    12001 ccgcgccacc catgatgaga ccgaggataa tttccggctg aaccaaagac aatttggcaa
    12061 caacatttcc gaagagatgt tcgagcagaa tgataatacc gaacaccatg gtggttgcgc
    12121 cgacaacggc ggttccgatc aacacgggtt tggctgtggc tttgaaggta ttgccggcgc
    12181 cgtcagcaga ttccaggaag tgcttggcca gttcaaaatc gggcgcaatg ccgtattcct
    12241 tcttcacaac gtcttttgcg tcgggacgtg actcaatcaa tgaaagttcg taaatggact
    12301 gagcgttgtc cgaaaccggt ccgaagctgt ccacagcaat ggtcacaggg cccatgccca
    12361 ggaaaccgaa ggccaccaga ccgaaagcaa acacgggagc agcaaaggtg aattccggag
    12421 gcatcaatgc cataacggcc ggattcttgg ataccatgta agcaatgaac attagaatca
    12481 aaatggtcag gccttcccag aaagcagaga agttaccggc aaccagaccc gacagaatgt
    12541 tcaaagaagg accgccctgc ttggaagcat taacggtttc ttcgcaatga cgggaggacg
    12601 tactggtgaa tactttggtg aattccggaa tcaatgcgcc ggcgatggtt ccgcaactga
    12661 taatgatggc cagcgcccac cagagacttg tgtaaggttc agtcagatcg cccagaaggt
    12721 aataactggc gacaaacgta accgctatgg aaacaataga ggtaatccag accaggttgg
    12781 tcagcggatg ttcaaagtta aattctttct tgccaccgaa agccgccttg ctgataacgt
    12841 cattgacaaa ataagaagca agagaggtca agatcatgag aattctcatg gcaaaaatcc
    12901 agacgatcag tttaccgccc atttctggat tgctggccaa agccagagcc agaaaagcaa
    12961 tcaacgcaac acctgtcaca ccgtaggttt caaaaccgtc tgccgtgggg ccgacgctgt
    13021 cgcccgcgtt gtcaccggta cagtcggcaa taacaccggg gtttttggga tcgtcttcgg
    13081 gaagatggaa aacgatcttc atcaggtcgg aaccgatgtc cgcaatcttc gtgaagatac
    13141 cgccgcagat acgcagagcg gaagcgccca gcgattcgcc gatggcaaaa ccgataaagc
    13201 tggggccggc cagttctttg ggaatgtagg ccagaatgat aatcataaag aagagctcaa
    13261 tgctgaccag aagaaggcca acgctcatac cggaacgcag acaaatgtta acgatgttga
    13321 gagggttgcc gctcaatgaa gaaaaagcgg cgcgggagtt ggcgacggta ttgatacgga
    13381 taccaaacca ggccaccgtg taggacccca gaatacccaa gatcgagcat atcagaataa
    13441 tgatgatgtc cgaaactccc tttccctgca gagcgccaaa atagtaaacg atacagatcg
    13501 caatcagaat ccacaacgcg atgagaaatt tgccctgctg gaacaaatag gttttgcagg
    13561 tctcccaaat ggtctgggaa acatccagca tcgccttgtg cgcgggcagg tttttggttt
    13621 gcacatactg catgatgccg aaagccattc ctattaaaca gatgatcaat cctacattga
    13681 ggattgtcaa tccctgaaga gacccgccca taaatgcaac ttgggtcaga tccggcaatt
    13741 ggatgtctgc ttcaccggca aacgctgcgc ccgccatgag caaaaacatc aatgtgctca
    13801 gtaaaaacgt ccagatactt tttttgtttt taaacattcc attcctcctt taaaattact
    13861 taatattttt tgtatggtat ttagccatag cttgttgcat ccctgatatg acaatgtccg
    13921 cagcagcttc cgctgccagt tgaatgcttt caggaagcac accctgttct tcctgattaa
    13981 acttctgcaa aacgtaattt tcaacgccgg ctttgtccgc cggtttgccg atacccattc
    14041 tgattcgcag gaaatccgcg gatcccagag cggtgataac cgatttcagc cctttatgtc
    14101 cggcatctcc tccgcctttt ttgagacgaa tcgttccgaa cggcagatca agatcgtcat
    14161 gaatgacgat gacatggtca atgtccacct tgtaataggc cattaatcga ccgaccgatt
    14221 tcccgctaag attcatgtag gtctgaggca tcgccagcag tacgctaacg ttgttgatct
    14281 tacctcgatt ccaaagagca tcaaaacttt tttgcgttaa actaattttc cattgggcgg
    14341 caattttttc caacaccatg aaaccgatgt tgtgtcttgt ccgtgcatag cggcttccgg
    14401 gattgcccag tccgatcatc agatacatga tttgctccct ctcgtcaact tgccggaaaa
    14461 atattttctt taaaaccgag agaagcaccc gctatcaccc aattattgca cttttctata
    14521 aagttacttc cccttttctt tttctgccgg agcagccgca gcgccagctg ttgccgctgc
    14581 ttcaccttcc gccggagcgc cttcagtagc gccttcctct ttggcagcct cttctacctt
    14641 ggcaaccttg atcatggcaa cagaggcaac cgacgtatcg ggtctgtcca gaagcgttac
    14701 cccttcagcc actttcagat cccggatctt aacggaatcg ccgatttcca gatcggtcac
    14761 atcaacatcg atatgatcgg gaagatccag cgggaggcag gaaactttga cctctctttt
    14821 caagtgctgt aattcaccgc cgttttccac gccgatggct ttgccgatga aattcaacgc
    14881 cacatccatg gtgatcttgc gtttttcatc cacttcatag aaatcgacat ggtagagttt
    14941 tccggtcagc ggctgaagct gcagttcttt aatcagagac agtttttcaa tttttttgct
    15001 tccttcatcg tcaatgatca gtttaatgaa tgcgtgatct tttttgtctt tgtgcagttt
    15061 aatcagttca tcgcttttta cagccaacag aatattttca gcagagcggc cgtaaaaaac
    15121 cgccgggaca aatcccttat cccgtaaacg gcgggatggt cctttctttt gttctttacg
    15181 gacttgagcc gctaaatctg ttacctccat ttgttcctcc tcatataaat tcctatataa
    15241 atagtgagct tacggaatca ttgtaataaa tacgacgcac cgcttcgccc agaattccgg
    15301 cgaccgataa tacttttatt tttttacaat gcttggcccg atcgttaagc ggtatggtat
    15361 cggtgacaat cgcttcttta atatcggaat tttcaatgcg atccagagcc ggtcccgaca
    15421 acacagggtg cgtacaacac accgaaattt ccagcgcgcc tgctttcttc atggcgcttg
    15481 cagcctgcac aaccgttccc gctgtatcaa tcatgtcatc cagaatgatc accctttttc
    15541 ccttgacatc accgataata ttcatggctt ccgcctcatt ggggccttct cttcgtttat
    15601 cgataatcgc cagagatgcc cccagtcgtt ttccaaaaga tctggccctt tccacacctc
    15661 cggcatccgg cgaaacgatc accgtattat ccatgtaggt ttttttcata tagtccagca
    15721 gaaccggttt ggcaaaaaga ttatccaccg gaatattgaa aaatccttga atctggcccg
    15781 catgcaaatc catcgacagg acgcggtttg ccccggcggc cgtaatcaaa tccgccacca
    15841 actttgccgt aatgggagcg cgggaggcta cttttcgatc ctgcctggcg taaccataat
    15901 acgggacgac tgcagtgatt ctatcaacag acgccctttt cattgcatcg atcatgatca
    15961 gcaattccat caatgttaca ttgaccggcg ggcacgtaga ctgaatgata aagacatcca
    16021 ttcctctgac attttcatcg atttctacgc gggtttcccc gtcgctgaag gtggtgacat
    16081 tggcctttcc cagagacacg cctaatgtat tgcaaatttt ctcagcaagt tctttgttgg
    16141 catttcctga aaaaattcta attctttcaa gcatttaaaa aatactccgc atttttttct
    16201 ggctggggcg ggaggattcg aacctccgaa tgcaggaacc aaaatcctgt gtcttaccgc
    16261 ttgacgacgc cccaataaac atatttctca atgaggggta tcgatcacaa tctgattagg
    16321 aatatggaat gattacaaag acttggcaaa aaataccgta taattaccgg gaatttcctt
    16381 atttatgcat tccagggctt tttttgcctc ttttccgttc ctaaatatcc caaagattgt
    16441 ggggccgcta cccgacatca atgcgccaag cgcaccatgt ccgagcaaca tttttttaaa
    16501 ttcatccagt tccggataaa ttttcaacga aactgactcc aggtcatttt gtaatacctg
    16561 aactatgtcg cccagtttca aaaatcgtgg aatgctataa ttattttttc ccattgtcaa
    16621 tcttaaattg aggctttcgt aaaccatttt tgtggaaata tcgaaaggag gcttaatcaa
    16681 aacaagattc agaggaggca gattgcgtaa gtgcttgagt ttatcgccga ttccggtagc
    16741 cagagcggta tttccaaaaa caaaaaaagg gacatcggct cctattttgg ccccgattgc
    16801 catcagctca ttttttttta gttttaatgc acatattttg tttaaagcca gcagtgttgt
    16861 tgcggcatcg gagcttcctc cgcccagacc ggcggtcatg ggtattttct tgatgagagt
    16921 aatttcgatt ccgccggaat aattggcata ggcaaaaata gcctttgcag cgcgcacaat
    16981 gagattcccg tcgttttcgg gcagattcat ccccggacaa tgcagaacaa tgccccgatg
    17041 aagaggagcg aaaatcagtt catcataaat actgattttc tgcatcaatg aaaaaatgtc
    17101 atggtatccg tcagcgcgtc gtcgcagaat cttcagaaaa agatttatct tcgccggcgc
    17161 aatccagtgc atatcaaccc ttttctttga cgtaacgcat tttcagttca tagatgatcc
    17221 cctgaagcag gccgctttca tttttgtcaa gatttccctt tgtcttttca ttgagcatat
    17281 cgagaatatc aatcgtctgt ttggccgccg gcaaattctt ttgcggcgcg gcgtcttccg
    17341 attccgccag atcgccaaaa tgaaaaaacg cggacgtact taatgaaaga atgaaattgg
    17401 taaagttcac cggcggataa tcagattcac cctgtttagt cggctccggg cctttttgtt
    17461 tttcttcagc tcccgccgtt ttggtttctt cctgtttttg ctcttcggct gattctccga
    17521 aatgacgttt gtctttaatc acaaatccgg agccttgctt cttgtcttcc atgaattcct
    17581 tcctcccctt ttctgcgaaa aatatttaag actttttcgt caacgttatt tcgccttttt
    17641 tggattgatc gattaaaatt ttatcgcctt cggcaaattt ccccgccaga atctccatgg
    17701 ccaacgggtc cagaatcagc ttttgcagcg agcgtttcag cggccgcgca ccgtaaaccg
    17761 gactataccc ctgcccggct atatagtttt tcgctttttc tgtcaattcg acgctcatct
    17821 tgcgttcctt gagacgctta tcgatgagac ccatctgaat atcaacaatc ttgtaaatat
    17881 cctccagctt caacgccgaa aacatgataa tatcgtcgat acgattcaga aattccggtt
    17941 tgaacgtggc gcgcagcgca tccatcgacc ggtttctctt ttcttcatca tccagcgacg
    18001 gatcctgaat ccactgacta cccacattgg aggtcataat gaccacggta ttcttgaaat
    18061 ccaccgtccg tccgtgtcca tcggtcagac gtccgtcatc cagaatctga agcaaaatat
    18121 tgaagacatc gggatgcgct ttttcaattt catcaaaaag caaaacgctg tagggtttgc
    18181 gtcgcacggc ctccgtcaga taaccgcctt catcgtatcc gacataaccg ggcggagcgc
    18241 cgatcagacg ggccaccgcg tgcttttcca tatattccga catgtcaata cgaatcatgg
    18301 cctgttcatt atcgaacatg aattccgcca gcgcgcgcgc cagttcggtc ttgcccacgc
    18361 ctgtcggccc gagaaaaatg aaagaaccga tgggacgatt cggatcctgc agaccggaac
    18421 gcgcccggcg aagagcggcc gatacggcgt tgataccctc ttcctgaccg atgacgcgca
    18481 atttgagacg ctcttccata tgaagcagtt tctgaacatc actttccatc atccgggaaa
    18541 gcggtatccc cgtccagttg gaaacgactt cagccacgtc ttccgcatct acttcctcct
    18601 tgagcatctg attgtttttc tgaatatctt cgagtttttt ctgctcgttc tgaagttcct
    18661 tatccaacgc caccaattta ccgtaacgga tttccgcagc cttttcgaga ttgccatccc
    18721 gctgggcgtt ggcctcctcc gtcttcaggg attctatccg gcttttaata ttttgaatct
    18781 ttttgatgat atctttttca ccagaccagt gtactttcat ctggtccatt ttttcctgaa
    18841 ggtcggcaat ttccttttca atcttgtcac gcctctcttt agcggtctta tccgactctt
    18901 tcttcaacga ctgaagctct atcttcagtt gaatgattct gcggtcgatg gcgtcaatct
    18961 ctgaaggcat gctgtccagt tctattctca aacgcgacgc cgactcatca atcagatcga
    19021 cagccttatc cggcagaaaa cggtcggtga tgtagcgatg agacagcgtc gctgccgcca
    19081 caatcgccga atctttgatc cgcacgccgt gatgaacctc gtagcgttcc ttcaatccgc
    19141 gcaggatggc aatagtatcc tcgacggtcg gctccttgac cagaaccggc tggaaacggc
    19201 gctccagcgc cgcgtctttt tcgatgtact tgcgatattc attcaacgtt gtcgcgccga
    19261 cacagcgcag ttcgccgcgc gccagagccg gtttgagcat attggaagca tccatcgacc
    19321 cttccgaagc gcccgcgccg accatcgtat gaatttcatc gataaagaga ataatctcgc
    19381 cctcggcgct gatgacttcc ttcaacacag ccttgagtct ttcttcgaac tcgccgcgat
    19441 atttcgcgcc ggccaccaga gcgccgatgt ccaaaccgat aacgcgtttg tttttcagat
    19501 tctccggcac atcaccgtta acgatacgct gcgccagacc ttccacaatc gccgtcttgc
    19561 cgacacccgg ttcgccgatc agcaccggat tatttttggt gcgccgtgag agaacctgca
    19621 tgatccggcg gatttcgtcg tcgcgtccga tcaccggatc aaaagaacct tttctggcca
    19681 gcgcgttgaa atcgcgggca tagcgctcga gtgcctgata ttttccttcc ggattcggat
    19741 cggtcacgcg ctgattgccg cgaatatctt tcagcacctt gaaaatattg tctttggtca
    19801 caccggcctg acgcagaatt tttccggcct ggccttcctt ttcctcggct atacaaacca
    19861 gcacatgttc ggcgcttaca tattcatcct tgagctgagc cgcttcggtc atcgccttgt
    19921 caaatgtttt attgagcctg ccccccaggt aaacttgtcc cgcgccggca ccgccgacac
    19981 tcggttgttt tttcagataa ccgtcgagtt ccttctcgat tttttgtgat gaagcattga
    20041 gcttgttgat gatcgccccg gcaatgccat cctcctgtcg caagaaacaa accagcagat
    20101 gctccggttc gattgactga tgccccatgg aaccggctaa tgtttgcgct tcctgaagcg
    20161 cttcctgaac tttcaatgta aatttatcaa aacgcatatt gtttaccttt tatcaaaatc
    20221 taattttcaa atctccattc gctttaatga gccttgattt tccgaaacgt agtaatcgga
    20281 aaatcttaga gcgaattatc ccgccacctt cagttggcag ggacgagcat cacaaagctc
    20341 gctctcagcg agtatcctta ccctacatcg cttcgctcct aggatagttc ggcttacgct
    20401 tcagatttcg tttcacccgt cttaccacgg cactactcgc ctggtataat tcgccctgca
    20461 agcttcagca cacttcgttt ctgaagcttg ggtctcatga tcagattgcc tcgctgccac
    20521 ttacgcttgc tatagtcagc gagccttgtt actcaacggg tttttcaatg gtcacaaaga
    20581 gcttgccgtt tttgaatatc gtcacgtttc ccgaagattc ggaaatgacg atggctacag
    20641 ctctggtcaa actggtgata ccggccgcgg caatatgacg gctgcccaga ccgggcggga
    20701 aatccttgct atccagagcg gcgctgagat gccttccggc tgtgaccagc acgccgtttt
    20761 ccttgatgat aaacgctccg tcaatggccg agagctcctt gatcgtctct tccaaatccg
    20821 gattcagaat attgagctga tcttcgggat accccaaaaa cgggttgatg atcatctgtc
    20881 gggaaagctg cataaccttc tcgtcatcac ccagaataaa gatggttccc acctttctgc
    20941 tttctctgcc ttgagcggcc agctccagcg ctatattcag cacagcatta aatacttccg
    21001 ggcgcacatt ttccagaaca tcattgatga aatccgacgt tagaatctca aattccttcc
    21061 ctacatcgat cacaaaaatg ctatcggcgt aaccgaattt cggcaccccg cttaaacaaa
    21121 ccaccttatc gcctcttttg agggcaccca gaatgattcc cttggcaatg gcaattttta
    21181 ttattcccac gcgggtcaaa ttcacattcg ggatataaag caccttcgcg tcttttttca
    21241 tttcttccga taggctttcg tcctcgcggg aaaccataat gaaagcgata tctttttcta
    21301 tttcatccat aatcagactg acatcactga ccacatcagc acagacaagc agtgcgttgg
    21361 ctttgatttc acgtgcaatt ttgacggcat gatcaatcat tgttttattt attgttttca
    21421 ttgatattca ccttgttttt tattgttttt agaataagca taaaaaacgc ctctgtcaag
    21481 gttggatcat tcaaaaaaac actgatttag atgcatttat ccaccggttt ccgatctttc
    21541 tgtcacttga ttcattctgg acttttttca aaaaagaaaa cttgaccgta aaattatagt
    21601 tggctagtat ttataaatta ccggcaaagg agacgtgaca gcattggaat ttcccgtagt
    21661 aaccggcaca cttaatttat atatagcgga aatcaatcgt tttcctctgc tgacggctga
    21721 ggaggaattt aaactggctg tgcgcctgaa aaagtacaac gatgtctccg ctgccgaaaa
    21781 gctgatcgtt tccaatttgc gttttgtcgt caaaattgct catgaatacc gaaactacgg
    21841 tttcaaactg tctgatttaa ttcaggaagg caatatcggc cttattcatg ccgtaaagaa
    21901 attcgacccg tataaaggat acagactgat ttcttacgcg gtctggtgga ttcgcgccta
    21961 tatccagaat tatcttatcc gctcatggag catcgtcaaa ataggaacca ctcaggctca
    22021 gcggaagctt tttttcaaac tgaatcaaac gaaaaaagaa ctggaaaatc tttcccggaa
    22081 aaatccggag ttcagtgaaa ttgccacatc tctgggagtt aaggattccg aagtggaaga
    22141 aatggacctg cgcatgggag cgcgagatgt ctccctgcat gagatcatca acgacgaaag
    22201 cagcaactcg cacatcgatt ttctggcgta cgaaggagac gatcaggagg tctccctgat
    22261 aaaaaaagag gagaaaagcc tggtgcaacg gaacattgcc ggagcgctgg ccaatctcaa
    22321 tgagagagaa agttacatta ttctcaaccg cgtcatgtca gacaatccga aaacgcttca
    22381 ggagatcggg gaaaaataca atatttcgcg ggaacgggcc cgtcaaatag aaaaacaggc
    22441 cctgaaaaaa atgcaacttg cattgccata ctttaaacct aacaaataac acaatcgtta
    22501 caacaggcag gtttttacga aaatattttt caatattttt ataaaaacgc tttagataag
    22561 agatggaatc caaatcatcc tgcgccataa cagggggcac acattattgg aaaaaataat
    22621 tttttttaaa tactttcatc gtttaaaaag cagactggcg ggcaaacatc agaatgaaaa
    22681 actgacggaa gacgaactcc tgcgttcgga attattcagc gccaagcaga tgcgacaaca
    22741 cggcaaaacc attgccggtg ctcataaact gaacgaaggt ctcacctcaa accagttgct
    22801 tgaacgatta acggaaaatg aaaaggtgct tttccaggcc cgggaaaaat tgaccaaagc
    22861 ggtgctggag aaccgccgcg taacaccggc ggaagaatgg tttctggata acttttatct
    22921 gattgaagag cagatccgaa cggcaagacg acacctcccg aaagtctaca gcagagagct
    22981 gccccatctg ctcaacgggc cgtcagccaa tcttccccgc gtctatgaca tagcgctgga
    23041 aatgatatcc cacggcgatg gtcgggtgga ttcgaaaacc ctgagcctgt ttgtttcttc
    23101 ctatcaatcc gtcaccgtac tgaaaatcgg tgaattatgg gcaatcccta ttatgttgcg
    23161 cctggcgctg atcgaaaaca tccggcgtat cgcgtcacgt atctccaaag acagaaccaa
    23221 ccgggacctg gccgattact gggccagaaa aatgatcgcc accgttcaca aggatccaaa
    23281 aaatcttttt ctggaaatcg ccgatatggt ccgttcggaa ccgccaattg ccggcccctt
    23341 tgttgcggaa atggcccgtc agctgcaggg acagggcccg gctctggcat tgccgctgac
    23401 ctggatagag cagcagcttt ccgaatacgg tcagaccatt aaaaatttcg tccagatgga
    23461 aagtcagcag caggcggctg accaggtttc catgagcaac agtatcgtca gtctgcggtt
    23521 tctgggaacg atgaactggc gggaattcgt ggaaaccatg agtttcgtgg atcaggtcct
    23581 gcgcgaggat cccgcggata tttatgtcaa aatggatttt gccacccgcg accggtaccg
    23641 tcatgtcata gaaaacatcg caaaaaaaag cagtctatca gaaaccgaag tggcgcgtat
    23701 ggcgatattg ctcgcccgta agacatcaaa ccagaagggc ggccaggacc gtacggcaca
    23761 tgtcggtttc tatttaatcg acaacggatt gccggaactc gagcgtctga ccaagatgcg
    23821 tttctccctc ttcgagagcg ttgcccgttt cagccggcga tttccgctgt tgatttatgt
    23881 cggttccatc ttcctgctga cgtttacagc ggcttttctt ttgttagcaa tggcttatgc
    23941 ggacgggctg accgggtggc tgctggctgt tatctggctt gtggcccttc tgagtacaag
    24001 ccatttaagc atagcgctga tcaattgggt gtcgacgctt ctgataacac ccgctccatt
    24061 gccgagaatg gacttttccc gcggcattcc tcccgaatac cgcaccctgg tggccattcc
    24121 gacaatgctg ctgaatgata agaacgtcgg caaactgatt gaatcgctgg aagttcgttt
    24181 tctggcgaac cgcgacgaaa atctgcgctt tgcccttctg accgattttt gcgacgcgaa
    24241 aaccgaaaaa atgtccgatg acggccagct tctgcgcctt gttcagaaag ggattgagag
    24301 cctcaacgaa aaatacgctg atgaccgggg agatattttt ttcctgtttc accgttcacg
    24361 ccgctggaac ccgaaagaaa atatctggat gggttatgag cggaaaaggg ggaaactggc
    24421 tgaactgaac gcctttctgc gcggtatcac gcacggcccc gaaggagggt tgttctcact
    24481 gattgtcggc gacacacagg aattaagaaa tgtcaaatac gtcatcactc tcgatacaga
    24541 caccaaactg ccgcgcgatg cggcatggca gtttgtggga accatggctc acccgctcaa
    24601 tcgggcgcaa tatgacgaac agaaaaaacg cgttgtcgag ggatacggca tcctgcaacc
    24661 gagggtctct gtttcgcttc ccgatatcag ccggtcgctt tacagcatca tgcacggaag
    24721 cagttccgga atcgatccct acaccagggc ggtttccgat gtgtatcagg atgttttcgg
    24781 cgaaggttcg tttattggca agggaattta cgatatcgat gctttcgaag catccctgaa
    24841 cgggcgcttt cccgaaaatt atattctcag tcatgatctg atcgaaggct gctatgcgcg
    24901 atcgggcctc atcagcgatg ttcagctcta cgaagactat ccgtcagcat acaaagccga
    24961 tgtcagcaga aggcaccgct ggattcgcgg agactggcag atcgcccgct ggatcatgtc
    25021 cagggttcct tccgctgatg gggaaaggct cggcaatcct ctttccgtgc tttcccagtg
    25081 gaaaatcttt gataatttgc gcagaagcct tgttccggca tcacttacgc tcatgctgat
    25141 tctggggtgg ctgctgtctt cctcgcccgg cttgtggaca ctggccgtgt tgagtatcat
    25201 tgtgtttccg gcccttgtca tgtcgttgat gagcgttttt caaaaacagc agaatgtgat
    25261 tctgacgcat cacctgtcct cagtcttcca gaaaaccggc aataattttt tacaggtttt
    25321 gttttcattt atttgcctgc cctacgaagc ctatttcaac acggatgcct tgctgcgcac
    25381 gctctggaga atgctgattt cgcacaaacg gcttctggaa tggacgccgt cggaaaacac
    25441 gaacaatcaa cgcgacgatc ttgccggtta taccaggacc atgtggtttg caccgtctct
    25501 tgccgccgca gtattcgctg cattattgac agcagttccc accggcgtac cgtccgcggc
    25561 gttttgtctt attctctggc tgctttcccc ggccgtcgcc tggtggttca gccagccgtc
    25621 gcgactccgg gaagccagac tttcttcagc ccaaattaat tttctacgaa aagcatccag
    25681 aaaaacatgg gcgttttttg aagaattcgc cggtcccgaa gacaactggc ttcccccgga
    25741 caattatcag gaacaccccg tggaagtcgt cgctcaccgc acatcgccga ccaacatcgg
    25801 cctgtcgctt ctggcgaacc ttaccgcata tgattccggt tacatcaccg ttttaaaatt
    25861 tattgagaga tcatccaaca ccctgcgaac catgacctcc ctggaacgat ataaaggtca
    25921 tttttacaac tggtatgaca cgcagtccct ggatccgctg ttgcccctgt acgtctcatc
    25981 ggtcgacagc ggcaatctcg tcggctacct ttttatttta aaatcgggac tgtcttcctt
    26041 gcccgataaa aaaatcatcg acccgcaaat acttgacggc ctcaaggaca cgctgggaat
    26101 cctgaatgac tctacgggaa aatcagtttc cgcaaatctt tcccaaatca gcaagaccct
    26161 ggatgacgcc acggcttcgc cgccggccag cctgacggaa atgaaaatcg tcctggagaa
    26221 aataatatcg ctggctgaag aaacggcatc atgcatgcgt ccggaaagcg atgcttcaaa
    26281 cgccgccggc cactggttaa aggccttcct tcagcaggca acagcggctc ttgacgaaat
    26341 gctctttctc acaccatggc tgtttgatcc gcagttttcg gatctcatcc gggaagtgcc
    26401 cgaactcgat gacatcatga cgctgagaga attaacaagc ctttatgaac acgttgaatc
    26461 gaaaacagag gcaatgaaaa gattcgaatc gaccgccatt gacctctcgc gaatcagcga
    26521 cctgaaaaat ctgattgccg aatccgggaa ccgcgccgtc aatttgatgc aaaacatcga
    26581 cttgctggcg cggcaatgca cggaattcac cgatatcgaa tatgacttcc tttttgataa
    26641 atcctgcaat ctcctctcta tcggttataa cgtaacgaat tcccgccgcg atacaagctt
    26701 ctacgatttg ctggcggcgg aagccagatt cagtaatttt gtgggcatcg cgcaggggaa
    26761 actgccgcag gaaagctggt ttgccctggg caggctcttg accatggctc ccgggaaaaa
    26821 acctgtgctc gtttcatgga gcggctcaat gttcgagtat ctgatgcccc tcctgatcat
    26881 gccgacctac gaaggatcgc tgctcgatca gacctacaaa gcggctgtcg ccgcgcatat
    26941 cgagtacggc aaaaaacgct ccgtgccctg gggaatatcg gaatcgggat actatgcttt
    27001 tgacagtcat ttgaattacc agtatcgcgc tttcggcgtt cccggcctcg gtctgaaacg
    27061 cgggctggca gaagatttgg ttgtggcacc ttacgcttcc gctctggcgt tgatggtgga
    27121 accggagaaa gcctgtctca acctggagaa acttgcctcc agggaatttc agggcaaatt
    27181 cggattttat gaagccatcg attttacgcc gtcgcgtctg acacggggcc aatccaatgc
    27241 tgtcattcga tcctatatgg ctcatcatca gggcatgacc ctgctggccc tgacttactt
    27301 gctgctggac cggccgatgc aaaaacgatt cgaagccgaa ccgctatttc agtccgctct
    27361 attgctcctt caggagatgg tccccaaagc cgttgcgttt tatgcatcgc cttccgaact
    27421 catcagcgca cagaaagaac cggaaatctc cgaaaaccct gtacgcgttt tcaacacgcc
    27481 ggacacgacg gttcccgaaa tacaactgct ttccaacggc cgctatcaca tcatgctgac
    27541 caacgccggc ggaggttaca gccggtggaa aaatcttgat gttacccgtt tccgcgaaga
    27601 cgtcacctgc gacagctccg gcattttctg ctatcttagc gacgttaaaa ccggcgacat
    27661 ctggtcaacc acctatcagc cgacgcttaa aaaaccccgc aattatgagg caattttctc
    27721 ggacggccgg gtggaattcc ggcgtcgcga ttatgaattt gacgtgcaca cggaaatcgt
    27781 cgtttcaccg gaagacgaca tcgaactccg gcgggtcacg attaccaacc agtcacgatt
    27841 gcaaaaaaca attgatatca ccagttatgc ggaggtggtc atgacacact ccgctgcgga
    27901 caagctgcat cccgcgttct ccaatctttt tgtccagacc gaaattatcg agcagcgccg
    27961 ggcgattgtg tgcaccagac ggccgcgttc ggaacaggaa atcaatccct ggatgcttca
    28021 ttcgatggcc gtgcacgggc aggacatcga acaaatctcc tatgaaacgg accggtcccg
    28081 ttttatcggc cgcggcaata cactggcgga tccgcaggcc atgggccccc ccagcgatct
    28141 tttcggaact ctttccgaca gtcaaggctc ggtgcttgat ccgatcgttg ctatcagatc
    28201 ccgcataacc ctcgggccgg aacaatcggc cgtggtggac atggttttcg gcatcggcca
    28261 aacccgggac gaagccataa cgctcgtgga caaatatcag gatcgccgca ttgccgatcg
    28321 tgtttttgaa atggcctgga cgcaaagcca agtgcttctc cgacagatca acgcaacaga
    28381 agccgatgcc cagttgtatt cccatatcgc cagttcgctt atttttccca acgattccct
    28441 gcgtgccggc gccggcgtga tctcggaaaa tcgccggggg cagtcgggcc tctggggtta
    28501 cgcgatatcc ggcgatctgc cgatcgtcct gctgcgggtt tccgaccagt cccatatcga
    28561 actggtgcgc caactcattc aggctcacgc ctattggaga cttaaaggac ttgccgtcga
    28621 tctcgtaatc tggaatgagg agcatgccgg ttaccgtcag ttgcttcacg atcagatcat
    28681 gggtcttgta tcgtcgggct ttgtcgccga tctgaaagat cagcccggcg gaattttcgt
    28741 ccggtccacg gatcagatgc cggaagaaga ccgcattctg attcagacgg tcgctcatgt
    28801 tattctttcc ggcaaccggg gcaaactggc caaccaggtg aaacagcgcg gcggcttgaa
    28861 aacggccgcg ccccggatga tccccaatcg tacgcatcgg gaaccgccgg ttcccaaagc
    28921 ggcgcccccg cgctctgatc tggttttctt caacggcctg ggcggttttg atccgtcctt
    28981 cagggagtat gtcatcacct cgtcaaaatc ccagattacc cccgcgccat ggatcaatgt
    29041 tctggcgaac ccgactttcg gcaccgttgt ttcggaaagc ggcatgtcct acacctgggc
    29101 ggacaatgcc cacgaattcc ggcttacacc ctggcacaac gacccggtca gcgacagaag
    29161 cggggaagcc ttttatattc gggatgatga acgcggccac ttctggtcac cgatgcccct
    29221 gccccatcgc ggtctggaac cttacacaac ccggcacggc ttcggctaca gtgttttcga
    29281 acacacggag cgatgtattc agtctgaggt atggatctac accgccctgg atgcatcggt
    29341 aaaattcatc gttttaaaaa tcaagaattt aacggaacgg acgcgccgca tttccatcac
    29401 aggttacatc gaatgggttc tgggaggcct cagagagaaa aatgccatgc atatcatcac
    29461 ggaagcggat catcagagcg gagcaatttt cgcacgcaat tcttacaaca tggaatttcc
    29521 caaccgcatc tgttttttca actccagcgc gtccatacgg accattaccg gcaaccgcat
    29581 ggaattcctg ggacgaaacg ggacgctggc cgatcccgcc gtcatgaaac gtcagtatct
    29641 ttccggaaaa gtcggcgccg gcctggatcc ctgcgccgcc attcaggttc ctcttgagat
    29701 gcttgagggg gaagaaaaag aggttgtttt tacgctgggc atggggcgca atgttactga
    29761 ggctcgcaaa ctggcacagt ttttcagcga tccggcgacg gccgccgatg ctcttcaggc
    29821 ggttcagaat tactggagag agaagcttga tgctatccag gttcaaacac ccgatccgtc
    29881 cgtcaacgtt cttaccaacg gctggctgct ctatcagacc ctgtcctgcc gtctctgggc
    29941 ccgcacaggc tactatcagt cgggcggagc ctttggattc cgggatcagt tgcaggatgt
    30001 aatggcgctg atttacacgg aaccgaattt tgcccgggag caactgcttc tggccgcttc
    30061 ccgccagttt gaagaaggcg acgtccagca ctggtggcat ccccccctgg gccgcggcgt
    30121 gcgcacgcaa tgttccgacg attatctctg gcttcctctg accacctgct gctacatttc
    30181 ccggacgggt gatcagggca tcctggatga aatcatcccg tttatcaacg gccggccgct
    30241 caaccgggaa gaagattcct attatgatct gccggttcga tcgccggaaa aggcaaacct
    30301 ctacgaacac tgcctgcggg ccattaagag aggattgcgt ttcggcgaac acggattgcc
    30361 gctgatggga tcgggagact ggaacgacgg gatgaacatg gtcggcattc aggggaaagg
    30421 cgagagtgtc tggctcggat ttttcctcta tgacatcctg acacaattcg cgaaaatcgc
    30481 cagccggcgc ggggatacgt cttttgaaaa atgctgcagg aaagaggccc tgcaattgcg
    30541 cctgaatatc gaacagcacg gctgggacgg actctggtac cggcgcgctt tcttcgataa
    30601 cggttccatg ctcggttccg ccagaagcga ggaatgccgg atcgattcca ttgcccagag
    30661 ctggtcggtg ttgtccggag ccggcaacgg ccatcattcg cggctggcca tggaatcgtt
    30721 aagccagcat cttattcgcc gggatatcgg catcgtccaa ctgctgcatc cgccgttcga
    30781 taaatccggg ttgaatcccg gttatatcaa gggatacgtt cccggtgtcc gtgaaaacgg
    30841 cggacagtac acccacgggg cggtctgggc ggccatggcg tttgccgccc tgcacgacaa
    30901 caaccgtgcc tgggaaattc tgacgatgat caatcccgtc aaccatgcaa attctccgga
    30961 atcaatagcc atctacaagg cggagcccta cgttgtcgcg gctgatgttt acgcgttttc
    31021 gccgcatacc ggacggggcg gatggacatg gtacaccgga gcggccggct ggatgtacag
    31081 attgatcacc gaatctctgc tcggcttaaa aaaggaagac gaca
//
LOCUS       DAOOLB010000021        30362 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_1308_length_30362_cov_38.253440, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000021 DAOOLB010000000
VERSION     DAOOLB010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 30362)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30362)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30362
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1308_length_30362_cov_38.253440"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(67..1326)
                     /locus_tag="PLF77_04440"
     CDS             complement(67..1326)
                     /locus_tag="PLF77_04440"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPC08137.1"
                     /translation="MLLFGFPFMVTLLGSIILYIFIYMPEIAPKMLLVMVQQVLTGVT
                     VPALVCVPMFILAADIITSGESADRLIRMIKVFVGHLPGGLPITTNASCTLFGAVSGS
                     TQATVAAIGGTMRPMLLQAGYKSSFTLGLIINASDIAFLIPPSIGFIVFGVATNTSIG
                     MLFLAGIIPGLMILLLFSVYCYVYSRIKKIPTLPKSDWSERWSAIKEGLPVMGFPVII
                     VGGIYLGLFSPTEAAAASVLYAILLETLIYKSLSFQRLTEAFLRTSVITGVVFILVGA
                     GQAFSFLLGFLQIPAALLPPLFGEEPSMLKVILIIVFVYFVACMFVDPIVAIFILSPV
                     LQPYATGAGVDPILLGTLVTLQAAIGSATPPFGCDIFTAQLIFRRPYIEVIGHALPFL
                     LILILATIILIAFPEIALFLPKMAMAG"
     gene            complement(1353..1937)
                     /locus_tag="PLF77_04445"
     CDS             complement(1353..1937)
                     /locus_tag="PLF77_04445"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPC08138.1"
                     /translation="MKTNHSQSWIIDRMKRFNRNVGRIINAIEISILVFCVATLAVLL
                     IVNVFARTFFQSIYFAEEVATFLVILMTFTGVSYAVRKARHIRMGAFLDAMPPKLEKT
                     FIIIISLISAMVMAIMAWASWEYLLNAMSQKHMTPALRVPKWTFYVIIPIGFGLACIQ
                     YLRTVLKNFVEPDPWQSPDQKSEYKDETIPGETS"
     gene            complement(2008..3054)
                     /gene="dctP"
                     /locus_tag="PLF77_04450"
     CDS             complement(2008..3054)
                     /gene="dctP"
                     /locus_tag="PLF77_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HPC08139.1"
                     /translation="MKKLILLYIALLVLFPCEVLSANYTGKAIRAKLASEEIDGDFMT
                     VWAGKFSEHMKQWSGGKIRIDVYPYGTLGNTGDINELAQMGIVQFVFSDHAWISAFVP
                     QAQALALNYIFPPEKVPEILEWMVREGEFMPLLEKAFRQKGLVPLSIMFEGWQWVSAK
                     KQIRSLDAMQGVKLRLMSSKMLVENYKAYGASPTPMTYGEVYSGLQMGLIDAQVNPLF
                     AAHSMKFYEVQDHFIQLKNEPFISIPTVNKKYFDSLPADVQKEIRQFWINAIIPAGHW
                     INQRNAMDMKKMKSAKPSLEFYVLNDKAVSKFKAKAKKVYPVFIEIGGKGAREILDAL
                     INDIANAKKALKLH"
     gene            3482..3829
                     /locus_tag="PLF77_04455"
     CDS             3482..3829
                     /locus_tag="PLF77_04455"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPC08140.1"
                     /translation="MKGSIKTRHEARVKIIKAMAHPSRLFIIEELSKRECCVNKLTEM
                     IGADTSTVSKHLSVLKNAGLVFDTKRGNSIFYSLRCPCIMDFVACVEGVLSANAKERR
                     KILKRCTPQENLS"
     gene            3826..4059
                     /locus_tag="PLF77_04460"
     CDS             3826..4059
                     /locus_tag="PLF77_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPC08141.1"
                     /translation="MKIEILGTGCAKCQKLEAMIREAVSASGIEAEISKVNDIKKIMT
                     YGVLTTPGLVIDGEIKTAGKLPTADQIELWLKG"
     gene            4120..5292
                     /locus_tag="PLF77_04465"
     CDS             4120..5292
                     /locus_tag="PLF77_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HPC08142.1"
                     /translation="MKEWQKLAWIVGIFLGAYYIPWDSILIRQAGLEAFMMLQDYARQ
                     HVLTCLIPAFFIAGAISVFVSQAAVLKYFGAKANKILSYSVASVSGSVLAVCSCTVLP
                     LFAGIYTRGAGIGPATAFLYSGPAINVLAIILTAKVLGWQLGLARAIGAIFFAIITGL
                     LMAFIFRKDDAEHETGELFLPEEEGNGRALWQDALYMLTMVLILVFASFARPTENDGI
                     VWQMIFSVKWYLVIALVLVLAVMLKKWFVKEELKGWVDSTWGFAKQILPLLLGGVLVA
                     GFLLGRPGHPALIPEQYIQGLVGGNSLWANFFASIVGAFMYFATLTEVPILQGLIGSG
                     MGQGPALALLLAGPALSLPSMIAIAGIMGIKKTSIYCIIVVILSTIAGMTYGWMVA"
     gene            5420..6463
                     /locus_tag="PLF77_04470"
     CDS             5420..6463
                     /locus_tag="PLF77_04470"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPC08143.1"
                     /translation="MTNVNDFIGKYRNIITIALSLIGIVLMAYYDYCDTECSYLRGDL
                     LGIDLKWVGIAYMAIIIIFAAFKQTPFVRALLAAGLGVEVHLYAFQIQNNVYCPFCLA
                     FSVMLILSFIINYEVPSAWREKRGRMWLYFLGEVDFPMFKIHKLPLLIFSLLGYLTVF
                     LTFNGSVTPAYGQTPSGAIPSLGKGPYEIIIFTDYFCPPCYRIDTKAEPLLKELLATQ
                     RVKLTFVDVPFNRSTPVYAKYYLYAVQAHSDATSVFRVRKILFDAAQSKKIQKEADLV
                     AYLKNQKVAWKTMDEKSVFPSLTAVIQKNDIRATPSCVIKYSNKDSQKLIGDVEIWKG
                     LTELKARLSAGNK"
     gene            complement(6662..7027)
                     /locus_tag="PLF77_04475"
     CDS             complement(6662..7027)
                     /locus_tag="PLF77_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08144.1"
                     /translation="MSEEIHKKSHKEPDEKHLDKMTVKELREIASEIPHEKAVHEMKK
                     EELIAFIKETRGIKDDEPVKKKHIGKVKMTKPELKAKIRECKASRLKALESKETERAV
                     WFRHQISQFKKKLRRIAGA"
     gene            7245..7946
                     /locus_tag="PLF77_04480"
     CDS             7245..7946
                     /locus_tag="PLF77_04480"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HPC08145.1"
                     /translation="MSKRNVCITVVLMLLMTAVTCFSADKYQWKLVDTKDNCQIYTSA
                     VPGKDYIAAKATCLIPARIEVIGTILRDIPNYPEWMHDCKATKMLKTVDDEKDVFIFW
                     FRQHIDLLTDRDMILKSKTVIDMQNGTNLVYADSTNELPYDSGKGYVRMPSFYSLFTL
                     QWVDRERTRVTFMIDPNLGSGVPSGIANSKIKSTPFKTLQNMMKMVKKSKYIEAAKTS
                     KYNKMAEDFVKAGHK"
     gene            8178..8253
                     /locus_tag="PLF77_04485"
     tRNA            8178..8253
                     /locus_tag="PLF77_04485"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8212..8214,aa:Glu,seq:ttc)
     gene            complement(8318..8794)
                     /locus_tag="PLF77_04490"
     CDS             complement(8318..8794)
                     /locus_tag="PLF77_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08146.1"
                     /translation="MASKSKDVRIEQLRIFEKKLAVRLQQLEQKGISPEKAQNDAIVK
                     SLKAKIKQTKARIATFDKFVQQTQALAQAKAAKQAAVVEEVKASAEEAKPKAKKKEDG
                     AAKEPKKQPAAEDGAAKKAKKQPADGQEAAPKKAKKQPDEGDDAAPKKKATKKKEE"
     gene            complement(8895..10067)
                     /locus_tag="PLF77_04495"
     CDS             complement(8895..10067)
                     /locus_tag="PLF77_04495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HPC08147.1"
                     /translation="MKNRSVIKIESVAFGGSGVGRADNLVVFVPFSAPGDELEIEITQ
                     RKKNFLRGRIRNIIKPSPWRTDPVCRYYGVCGGCCYQHIHYAYQLAIKKKQVEDAFRR
                     IGKVDAPPVLETVASPRMYHYRGKARYHVGDDPQTGHIGFLDISGGKIVDIEHCAIMQ
                     ETINEQLSILRENRQNLWHKDELTIWSRYPDVTSEDQTSVVRIVQGKRILAPDDGFFQ
                     ANEYLTDRLVDEVCRLVRPGKAENAIDAFCGSGLFSIFLSPFAEDITGIEMDKESVKY
                     AKINAANLNARNVRFLDGNVEIVLREKFLSLGSKIDLIVLDPPRVGCEMTLLKTLMDL
                     QPSNIIYISCNPATQARDIKYLCEGGYHLQSLHPLDMFPQTEHIEVIGYLKRIMAL"
     gene            10218..11060
                     /locus_tag="PLF77_04500"
     CDS             10218..11060
                     /locus_tag="PLF77_04500"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPC08148.1"
                     /translation="MPERKSILCPNCRKLISMDEPSCPYCGLQRPGMHNIAGNIGKLF
                     MASNPVMIIIYVNVALYIFSIFLDPQGIFGGSGPFGILAPSNQSLFLLGASGTFPVIG
                     AHRFWTLISASFLHGGIFHILFNMMALYQLGPFIIREFGVHRFINLYIVTGICGFSAS
                     VISGTPFTIGASAAICGLIGAIIYYGKSRGDSYGEAIYKQAMGWVIALVIFGLIFRGI
                     DNWAHAGGLLSGIGFSFLMGYNDNKQETAWNKMLAYACILLTAAVLLWSVVNSLFIGL
                     NISI"
     gene            11127..14108
                     /locus_tag="PLF77_04505"
     CDS             11127..14108
                     /locus_tag="PLF77_04505"
                     /inference="COORDINATES: protein motif:HMM:NF019966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPC08149.1"
                     /translation="MSEKSDFPEPTLREGDELYGFTVTRIKTFPTIHTTAYEMKHNKT
                     GAKLLHLHCFDRENLYAIGFRTPPYDSTGLPHILEHSVLAGSEKYPLKDVFKELMRST
                     MQTFINAFTYPDKTVYPVASQIKTDFYNLARVYTDLVLHPRMLRETFLQEGHHLEFSV
                     LDDLSSDLIISGIVYNEMKGAYSSPDSLIYKTLQENVYPDSVYSFDSGGDPNVIPSLT
                     YEQFCEFHSKYYSPSNARFFIYGDIPTKDHLVFLSEILKDFNYIEINSAIGSQKRFTQ
                     PRSVVGGYPIGKDESVERKTVVNIAWLMSENTDFETSLILQIISALLVGSAASPLRKA
                     LIDSGLGEDLTPVSGVEADLKQLMFCVGLRNTQRSDAQKIEQLILKTLKDIIDSGYDT
                     QLIEGVLHQVEFHGKEIVRGSYPYGISLMGAVFQTWLYDGDPLIGLDFSQIIENIRRC
                     WADDPLIFQKMTEKWLLNNPHRVLAVMEPDPDFNEKKEAAYREKMDTLKASLCEEELR
                     QINAKAKKLKEFQNQQDPPEAASALPRLNMEDIPKSIEKTPTRNSLIGDVPVWEHDLF
                     TNGIAYVDLAFDLADIPEELQIYLPLLGKMMTGMGAAGLTYEEMAKRIALKMGGLGYN
                     LGAGFTANGDSTWQKMIFSFKALYRNLPEAMSIISDVLCRGDLSHEARMRDLISERKN
                     NLQSAILPSGHLFARMAAGAALTLPAYRDEQWHGRTQFKFVQATAGEFVSSKSALRDK
                     LETLKQTLFNKNNMVINVTAEHQGLEIAKEHLPCLLDRLPASSQAAPEEEPSLNPVFA
                     GISVPAQVSYVAYILKTPCYTDPASPLLMIAAKELSNNYLYKYIRVQGGAYGGMSSFD
                     ASMGLFSFLSYRDPRISETLQVFKNAQTFYSQNEISSPDMEKAIISTISMIDKPSDPA
                     SKGYTAMMRNFAGITDKIRQKFRDDILSATPQKLKKVLSGYFSQAFKSASVAVYSSPE
                     KLDEANRQLEDKLHIESIFEN"
     gene            complement(14213..14620)
                     /locus_tag="PLF77_04510"
     CDS             complement(14213..14620)
                     /locus_tag="PLF77_04510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HPC08150.1"
                     /translation="MADELLMEIVTPEKMIFSGNIEEVTLPGTEGEFGVLRGHEAFLT
                     SVEIGEMNYVKDGKKSYYAVNTGYVEVTGDKVTVLIETAEKSDQIDKERALKAKDNAE
                     SRLSQMNKDDVEFEIMRAALARAIARINVAEKQ"
     gene            complement(14642..16051)
                     /gene="atpD"
                     /locus_tag="PLF77_04515"
     CDS             complement(14642..16051)
                     /gene="atpD"
                     /locus_tag="PLF77_04515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPC08151.1"
                     /translation="MNIGKIVQVIGPVVDVAFEEGKLPAILNAITITNSAINDQEDNL
                     VVEVALHLGDNVVRCIAMDITDGLVRGMKAKDTGAPIMVPVGKEGLGRILNVVGRPVD
                     GLGPVNAQTKMPIHRESPTFLEQDTSVHVLETGVKVIDLLVPFPRGGKMGMFGGAGVG
                     KTVVMMEMINNIAMHHGGISVFCGVGERTREGNDLYREMLESGVIKQAALIYGQMTEP
                     PGARSRVALTGLAAAEYFRDVEGQDVLFFVDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATDLGGLQERITSTTKGSITAVQCVYVPADDLTDPAPATTFAHLDGTVVLSRPIAE
                     LGIYPAVDPLDSTSRILDPNILGEEHYKVARQVQVTLQKYKDLQDIIAILGMDELSEE
                     DKLTVSRARKIQRFLSQPFFVAAQFTGTEGKFVSVPDTIRGFKEILEGKHDDLPEQAF
                     YMVGGIEEAVEKAKKIGGE"
     gene            complement(16121..16996)
                     /gene="atpG"
                     /locus_tag="PLF77_04520"
     CDS             complement(16121..16996)
                     /gene="atpG"
                     /locus_tag="PLF77_04520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPC08152.1"
                     /translation="MASLKDIKRKVGAVQKTKQITRAMNMVAASKFKSAQLKMENFRP
                     YAGKFMDVLNSLALRVESMSHPLLAVRDPKRIRVICMTSDRGLCGGFNTNLIKATERF
                     LKEKAGEGKDVSMITVGRKGRDYFRKKANIIAQKTDVLSKFDMTLAVSIADEVINPFI
                     KEEYDELYLIYNQFMNVSIQKPVVVRLFPLPSIGQEIEDEPDKRVDYSYEPSEEVLLH
                     KLLPMYVHVLVYRALLETSAGENGARMAAMDNATSNCQDLISSLTLKMNKARQAAITA
                     ELMDIVGGTEALAKG"
     gene            complement(17070..18584)
                     /gene="atpA"
                     /locus_tag="PLF77_04525"
     CDS             complement(17070..18584)
                     /gene="atpA"
                     /locus_tag="PLF77_04525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPC08153.1"
                     /translation="METIKAEEISQIISEQIKSYEKKLDISETGTVLSVGDGIARVYG
                     VQNAMAMELLEFPGGILGMVLNLEADNVGVAVLGEVTYIKEGDIVKRTGKIAQVPVGE
                     ALLGRVIDATGKPLDGKGPIETKEFRRIEMVAPGVIQRQPVNQPMYTGLKSIDAMTPI
                     GRGQRELIIGDRQIGKTAIGIDAIIRQKDTGVKCIYVAIGQKKSTVSQIVERLKQHDA
                     LSYTCVVAGCASDPATLQYIAAYAGCSIGEYFRDNGQDALIIYDDLSKQAVAYRQISL
                     LLRRPPGREAYPGDIFYNHSRLLERAARVSQELGGGSLTALPVIETQAGDVSAYIPTN
                     VISITDGQVYLEPSLFFSGIRPAINVGLSVSRVGGAAQVKAMKQVAGTLKLDLAQYRE
                     LAAFAQFGSDLDKATQAQLDRGVRLVELLKQPQFKPMSLGQEIVVLFAGTRGFIDKYD
                     VEKVRIYEEQLLSFVESKHPDIMKEIEDKKIISPELEKKMKEIMTAFDSVFVAE"
     gene            complement(18588..19130)
                     /locus_tag="PLF77_04530"
     CDS             complement(18588..19130)
                     /locus_tag="PLF77_04530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HPC08154.1"
                     /translation="MISNISKRYARAFFDIAKEENQIEPYYKELDQFTLLISRNESLK
                     DFLANPVFEQAVKKGVVDNLIAKLKLSGMTANFIRLLVDKNRIDILEDIVVCYRQLMD
                     EALKKVRVNVKTAFALSDELRSLIGANLEKALGKTVDMTVEQDPTLLGGVVIGVGDTL
                     YDGSIKNQLNNMRNLLGEAR"
     gene            complement(19127..19705)
                     /gene="atpF"
                     /locus_tag="PLF77_04535"
     CDS             complement(19127..19705)
                     /gene="atpF"
                     /locus_tag="PLF77_04535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPC08155.1"
                     /translation="MKKTISLITGVFVLLVAVGIAFASAEAEGGHHGADWFGLFKKAF
                     NFVVLMGLLYWLLAAKVKEFFVGRRAEIKENLEKAVERKAEAEKKYREYSEKIDKAST
                     EIDGIIEMIKAQGVTEKQKIIEDAERTAKKMKEDAHARIEQEMKKATEQLKSQAVELS
                     VQMAEEILKKSITQDDHAAMVKEYVNKVVIKH"
     gene            complement(19702..20136)
                     /locus_tag="PLF77_04540"
     CDS             complement(19702..20136)
                     /locus_tag="PLF77_04540"
                     /inference="COORDINATES: protein motif:HMM:NF012645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HPC08156.1"
                     /translation="MVTVIPDYTLLVQMVLFLALVFFLNILLYKPILSIIDRRKKQLE
                     ESENEIRLFDENAEKKVAEYEEKLKQAKLKASEAKKEVIQEGANQAKNIIDAVRNEIP
                     VMAREFQQKMDKEVEKAKAVLDGNSRQLSLEIAQKILGRPVQ"
     gene            complement(20476..20853)
                     /locus_tag="PLF77_04545"
     CDS             complement(20476..20853)
                     /locus_tag="PLF77_04545"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPC08157.1"
                     /translation="MWEKKKDAEEIKAFLGQGAEFSGKLIFNGSVRIDGKFEGEIFGQ
                     GSLVVGQGATVKANIDTKSVYISGDVQGNIDVKEKINIHSTGKFSGDVRTPVFVMEAG
                     AIFNGMSHMDDVAQQKIEIPKAD"
     gene            complement(20926..22041)
                     /gene="rodA"
                     /locus_tag="PLF77_04550"
     CDS             complement(20926..22041)
                     /gene="rodA"
                     /locus_tag="PLF77_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPC08158.1"
                     /translation="MIFLKFDRRIFHYFDWILLSFVLGICVMGVLNIYSTGFSAAGGQ
                     SPFYLKQLQWIVVGLLFMTFVFFMDYRVIVEYAYIIYGFSIFLLVLVLIVGSSSHGAQ
                     RWLGGGFFAIQPSELMKVVIILTLARYFDNHKSSEPYTLKELFIPLLIVAVPFVLILK
                     QPDLGTALMLMVIFASIVFFMGVDKKSLIFVFAAGLVLMPAGWFFLKDYQRERLVTFL
                     NPERDPLGSGYHIIQSKIAIGSGEIFGKGFLSGSQTQLKFLPEQQTDFVFSVFAEEWG
                     FIGSMLLIGLFVVIIFWGLKIALQARDLLGTIIAFGVSALIAWEVFINIGMVLGILPV
                     VGIPLPFFSYGGSAMLSLMAAIGLLNNVSARRFILQK"
     gene            complement(22038..23927)
                     /gene="mrdA"
                     /locus_tag="PLF77_04555"
     CDS             complement(22038..23927)
                     /gene="mrdA"
                     /locus_tag="PLF77_04555"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPC08159.1"
                     /translation="MLNGHEPIEFKGKIRIITGIFLLAVCILIGRLGYLQIIKGEEFK
                     KKSENNSVRFRFIKPLRGLIMDRNGNVLVDNKPSFDVIYIPNKNKANELSVEKLKSLY
                     ESQSMVFPFDQAVLKNAKPYLPVKLDKNVSMEKIALVETNALNLPGIYIDVSPIRLYL
                     DGEILAPIIGYTGEISREEMEKSEGKYAYGDISGKHGLERFFDEYIRGRRGAELVEVN
                     VYGREIKNLGRIDPVSGYNMTLTIDADLQRAAWQAIEGKAGSVVALDPRDGAILAMVS
                     SPSFDPNLFNEGISSELWKQLQSNPFAPLSNKAISGQYPPGSTYKLIVAAAALEEGII
                     SPETKIFCNGFFTLGNRTYRCWKKGGHGHVNLHQAMVGSCDVYFYTVGKMLGVDKIAE
                     YAKRFGLGDLAGVDLPNEKKGLVPTKAWKLKKKKEAWQMGETISISIGQGFNLVTTLQ
                     LANAYAAFANGGTLWRPYLVKKIDAPDGKTYKDFPPVKKGELKLSNTTMEIINDALKA
                     VVNEPGGTGQNAKLAQIEVCGKTGTSQVLGLPENEKARRRKVLSAFHKDHALFASYAP
                     SKSPEIVVAVILENAGGGGAVAAPVARRVLSAYFEGKKKEKSAGKSEKPGESDNRRNL
                     DGDDR"
     gene            complement(23929..24423)
                     /locus_tag="PLF77_04560"
     CDS             complement(23929..24423)
                     /locus_tag="PLF77_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08160.1"
                     /translation="MFHYLLLLILSLLLIVLQSTVTDIVFSNSFVFEVSLIIIIYAGF
                     RFEMIKGLLLAFYLGFILDLIGGSVPGVLALAYIMIFLCSFFISDLLDTGKNHVIVLF
                     SFICVFLKETMLQTFYFLVFDIRMRPDIFSLILIQSFVVGLVAPAVFYLIDRTGIFLY
                     EKKA"
     gene            complement(24426..25253)
                     /gene="mreC"
                     /locus_tag="PLF77_04565"
     CDS             complement(24426..25253)
                     /gene="mreC"
                     /locus_tag="PLF77_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPC08161.1"
                     /translation="MVFLKKYKVIIIIAVLMVLVLVVISYHLKFGDDKSFLRKVALEI
                     SSPVYKIINGSIQGIKDAWLHYFLLVGVQEENKKLKKKMDELQAVIVSYQEGYLEAQR
                     LKKLLAIPDYHRYPFIAARVIGREQATLSRTVLINKGASDGLKNGMPVVAYPGLIGRL
                     TNVSRNVSRVLLFIDENSNVDAIVQRDRIQGIISGAGPRGMILKYIPKTQDVRKGDII
                     VSSGMGGVFPKGWLIGQVINVDRQNDGLFLKIDVAPFTDFSKLEELLILSSAETAVE"
     gene            complement(25456..26493)
                     /locus_tag="PLF77_04570"
     CDS             complement(25456..26493)
                     /locus_tag="PLF77_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPC08162.1"
                     /translation="MFFDYVLGKFSNDLAIDLGTANTIVYVKDKGIVLNEPSVVAVHQ
                     DARGMKKVLAVGNEAKNMLGRTPGNIVAIRPMRDGVIADFDITEAMLKHFILSVHNRK
                     SLVRPRIIISVPSGITQVERRAVRETVESAGAREIYLIEEPMAAAIGAGLPVAEPTSS
                     MVVDIGGGTTEVAVISLAGIVYSKSVRVAGDKIDEAIVQYMKRKHNLLIGERTAEIIK
                     MEIGCAYPFDEIKVGSIKGRDLISGIPKITETNSEEIREAINEQVTFIVDAIKDTLEN
                     SPPELAGDIVDRGIVLTGGGALLANIDILIREETGLPITITDDPLSAVARGAGMALEQ
                     INVLKDIALEV"
     gene            26678..28273
                     /locus_tag="PLF77_04575"
     CDS             26678..28273
                     /locus_tag="PLF77_04575"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPC08163.1"
                     /translation="MLKFFRKHARGWFMIAVIAIIIVVFVLYFGASRGGEHAQVVATV
                     DKKAITVNEVLNEYDKLLEVARLNLKENLTPELIKKMDLKTKAYNDILNREVIIAKAA
                     DLKIQVSDDELRNSILSMPALQTNGMFDERKYHQILRFNRLSAEDFETLQRADLTARK
                     IEFLVREGVKISDKEIYDLYALQNRKINVDFLRLAASDVKHKISPVESELEDYLKRNG
                     NLFRVPEQTKIKYLFFGADTFPADISDADIQDYYSTYQNKYKAKDGKPLPLAGVRNDI
                     INELKRAKGMQTAYTEARKAREEIYQDDNMDAYARKHNLAVRDSDFFMITKPPQDFAS
                     VKNFADALLNMQKGELSKILSTENGYYLLQVVDKKASYIPKLDTIANEVRQRFIETET
                     QLLAEKEAKSILESLKAGESMEKVASAKGLKIGQTGFFQPGNTIPGVGESPEAAEILM
                     TLSAANPYAEKPLFINNVYFILKFRETTRIDDNAFTAEKEIYRKILTAMKQEEAMQTW
                     LEGNKADMIKEKRIKIKKKAEDL"
     gene            complement(28459..28534)
                     /locus_tag="PLF77_04580"
     tRNA            complement(28459..28534)
                     /locus_tag="PLF77_04580"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(28499..28501),aa:Ala,seq:ggc)
     gene            28639..28725
                     /locus_tag="PLF77_04585"
     tRNA            28639..28725
                     /locus_tag="PLF77_04585"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28673..28675,aa:Leu,seq:caa)
     gene            complement(29011..29451)
                     /locus_tag="PLF77_04590"
     CDS             complement(29011..29451)
                     /locus_tag="PLF77_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08164.1"
                     /translation="MKDKKITGTIENMETGETHDIVPFHFFPCNCALNTIVKILHVES
                     FLHPDNSRQISVDCLALDSSGFVFKLSMEFNPESQEDEQKIRSDITEGKIMAASGLYS
                     VLTEDEGGIMLFDPACESLPPEYSLEEVEEVFRVNNMTDKNRLI"
     gene            complement(29711..30196)
                     /locus_tag="PLF77_04595"
     CDS             complement(29711..30196)
                     /locus_tag="PLF77_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08165.1"
                     /translation="MRYAGIGPGQKFKRALLLDLYRSGEVYTHGEGPGADVDVRQTVF
                     DFSNDPDPEGMFPSCASCHSINDLHLVEITEGEKKATILCVDCRSKKISTFDTSIPLP
                     MVSRTNLKQFIETKGIKKIDKSVNEYKNLLDAEFEDKWNAYKKGKPEQQELIKKHGSL
                     I"
BASE COUNT         8262 a         7344 c         6441 g         8315 t
ORIGIN      
        1 ataataatca taaaacaaaa aagcgggata cccgttccgg attccagctt ttcattttta
       61 ttgctcctat cccgccattg ccattttggg cagaaacagc gcgatctcag gaaaagcaat
      121 cagaataatt gttgcaagaa ttaatataag caaaaacggc aaggcatgac cgatgacttc
      181 gatgtacggt cgcctgaaaa tcagttgggc ggtgaatata tcgcatccaa agggaggtgt
      241 tgccgaaccg atggcggcct gtagtgttac taaagtgcca agtaaaatcg gatcaacgcc
      301 agcgccggtt gcgtaaggct gcaacaccgg gctgagaata aaaattgcga caatgggatc
      361 cacaaacatg caggcaacaa agtaaacgaa gacgataatt agaatgacct ttagcatgga
      421 aggttcttcc ccaaacagag gaggaagaag agcggccgga atttgcaaaa atcctaataa
      481 aaaagaaaac gcctgtccgg cacccaccag aatgaagaca accccggtga tgacgctggt
      541 tcgtaaaaaa gcttctgtca ggcgttgaaa cgaaagactt ttataaatga gcgtttctaa
      601 aaggatggca taaagcactg acgcagccgc cgcttcggtc gggctgaaca atcccaagta
      661 aataccgcct acgataatca cagggaaccc catgaccggc agaccttcct taatcgcaga
      721 ccatctttct gaccaatccg atttgggcaa cgtcgggatt ttctttatcc tggaatagac
      781 gtaacaataa acggaaaaca acaacaggat cattaatccc gggataatgc cggccagaaa
      841 aagcattcca atggatgtgt tggtcgctac gccaaaaacg atgaatccga tgctgggcgg
      901 aattagaaaa gcgatatcac tggcgttgat gatcaacccc aacgtaaatg aacttttata
      961 gcctgcctgt aaaagcatgg gccgcatggt accgccgata gccgccaccg ttgcctgtgt
     1021 ggaaccggat accgcgccga acagcgtgca actggcattc gttgtgatcg gcagtccgcc
     1081 gggaaggtgc cctacaaaaa cttttatcat tctgatgaga cggtcagccg actctccgga
     1141 tgtgataatg tctgccgcaa gaataaacat gggtacgcaa acgagagccg gaacggtcac
     1201 ccccgttaat acctgttgca ccataacgag taacattttc ggcgcaatct ccggcatata
     1261 aatgaatata tacaggatga ttgaacccaa aagggtgacc ataaaaggaa aaccgaagag
     1321 aagcatcgcg acgagtgtca aaccgaaaat tttcacgacg tttctcccgg tatggtctcg
     1381 tctttatatt cgctcttttg gtccggagac tgccaagggt ccggttcgac gaaattcttg
     1441 agcacggtcc tgagatattg aatacacgcc aatccgaaac cgatgggaat gataacataa
     1501 aatgtccatt tgggaacccg cagggcaggc gtcatatgct tttgcgacat ggcattgagc
     1561 aggtattccc atgacgccca agccataatg gccatgacca tcgcgctgat caacgatatg
     1621 atgatgataa aggttttttc caacttcggt ggcatggcat ccagaaatgc gcccatgcgg
     1681 atatggcggg ctttacgaac agcatagcta acgccggtaa aggtcatgag aataaccaga
     1741 aacgtggcaa cctcttccgc aaagtaaatg ctctggaaaa aagtgcgggc aaaaacattg
     1801 acaattaata aaacagccag ggtcgcgaca caaaagacaa ggatggatat ttcgatcgcg
     1861 tttattatcc gcccaacgtt tctgttgaat cttttcattc gatcgatgat ccatgattgt
     1921 gaatgatttg ttttcatgaa caccccttgg aacaacaatg actaaacact gatgcaatcg
     1981 aacattttaa ctgcgcatga taatttatta atgaagttta agcgcttttt ttgcattggc
     2041 aatgtcattg atcagagcat cgagaatttc ccgcgcacct ttaccgccta tttcaataaa
     2101 aacaggataa actttttttg ccttagcctt gaacttggaa acagctttat cgttcaaaac
     2161 ataaaattcc aaagacggtt tagcagactt catttttttc atatccatcg cgttacgttg
     2221 gtttatccaa tgaccggccg gaataattgc gtttatccaa aactgacgta tttctttctg
     2281 tacgtcagcc ggcaaactgt caaaatattt tttatttacg gtagggatac tgatgaacgg
     2341 ttcgtttttt aattgaataa aatgatcttg aacttcatag aatttcatgc tgtgggcggc
     2401 aaacaatgga ttcacctgag catcgatcag tcccatttgc aatccgctgt atacctctcc
     2461 gtaagtcatg ggagtcgggc ttgctccata ggctttataa ttttccacca gcattttaga
     2521 agacatcaaa cgcagtttta caccttgcat cgcgtctaat gatctaattt gtttcttcgc
     2581 actaacccac tgccagcctt caaacatgat cgaaagagga accagtcctt tttgacgaaa
     2641 ggctttttcc aaaagcggca taaactcgcc ttctctgacc atccattcca gtatctcggg
     2701 aactttttcc ggcgggaaaa tataattcag cgccaaagcc tgcgcttgag gaacaaatgc
     2761 acttatccac gcatgatcgg aaaaaacaaa ctgaacaata cccatttgtg caagttcatt
     2821 aatgtccccg gtattgccca gggtgccgta gggatacaca tcaatgcgga ttttaccgcc
     2881 ggaccactgt ttcatgtgtt cggaaaattt cccggcccag acagtcataa aatcgccgtc
     2941 gatttcctct gaagccagtt ttgcccgaat tgctttgccc gtgtaatttg ccgacagaac
     3001 ttcacaaggg aacaaaacaa gaagggcaat ataaagcaat atcaactttt tcatgatttg
     3061 gatccttttt ttggatttgt gagatggatt ccttttcctt aaaactatag aaaaaagaaa
     3121 atagcgagtc aatataataa ttaatgttgt aagaagcaca taaagggtcc gttttaaaaa
     3181 tgcccatttg atttataaaa atcgagcaag gattaggcga ataataactt tttatccacc
     3241 cattcggcga aggtttcggc catctgccgg gttttgcctg tctgcgaatg ataaacgatt
     3301 aatattttag ccatgttatt ttcctgtccg gcggcgatga tatattaaaa gcaagaatgg
     3361 cgtcaatggg attcttcggc gcggtgggtc atccctcttt tcgccaatcc atcaatgaat
     3421 ttattgattg acattatggc tatttggcta tattgccaaa taaaataagg agaaaacaaa
     3481 aatgaaaggc agtataaaaa cccggcatga ggcgcgggtg aaaatcatca aggccatggc
     3541 gcatcccagc aggcttttta ttatcgaaga attaagcaaa cgtgaatgct gcgtcaataa
     3601 attaacagaa atgatcggcg ccgacacatc gactgtttcc aagcatttga gcgtcctgaa
     3661 aaacgccggt ctggttttcg acacaaaacg gggcaattct attttctaca gcctgcggtg
     3721 cccctgtatt atggattttg tcgcttgcgt tgaaggcgtt ttgtcggcta atgccaagga
     3781 acgccgtaaa attctgaaaa gatgcacgcc ccaggagaat ctgtcatgaa aatagaaatt
     3841 ttaggaaccg gctgtgcaaa atgccaaaag cttgaagcaa tgattcgcga agccgtttcg
     3901 gcctccggta ttgaagcgga aatctccaaa gtgaacgata tcaaaaaaat tatgacttac
     3961 ggtgtgctga cgacaccggg cctggtaatc gatggtgaga tcaaaacggc aggcaaattg
     4021 ccgaccgccg atcagatcga actctggtta aaaggataaa caagaaaaat tgagttttca
     4081 ttcgtgaagc ctgcctatta tgcatcggac gaggtaaata tgaaagaatg gcagaaatta
     4141 gcgtggattg ttggaatatt tctgggggca tattatatcc cctgggattc gattcttata
     4201 cgacaagccg gcctggaggc attcatgatg cttcaggact acgcgcgcca gcatgtgctg
     4261 acctgtctta ttcctgcttt ttttattgcc ggagccatca gcgtttttgt atcgcaggcg
     4321 gctgtgctaa agtattttgg cgcgaaggcg aataaaattt tgtcttattc agtcgcttcc
     4381 gtttccggtt cggtgctggc tgtttgttcc tgcaccgtgt tgccgctttt cgcgggaatt
     4441 tacacccgcg gcgcgggcat cggtcccgcc acggcatttt tgtattccgg gccggccatc
     4501 aacgtgctgg ccattattct aaccgccaaa gttcttggct ggcaactggg attggcgcgc
     4561 gccatcggag cgattttttt cgcgattatc accggtctat tgatggcgtt tattttccgc
     4621 aaggatgatg ctgaacatga aacaggcgaa ctattccttc ctgaagaaga gggaaacgga
     4681 cgggcgctct ggcaggatgc gctttatatg ctgacgatgg tgttgattct tgtttttgct
     4741 tcgttcgcac gaccgaccga aaacgatggg atcgtctggc aaatgatttt cagcgtcaaa
     4801 tggtatctgg tgattgcatt agtgcttgtt ttggcggtga tgctcaagaa atggtttgtc
     4861 aaggaagaac tcaaaggatg ggtggattcc acctgggggt ttgccaaaca gatactgccg
     4921 cttttgctgg gtggagtact ggtggcaggc tttcttttgg gacgtcccgg tcatccggca
     4981 ctgattcccg agcaatatat tcaaggcctt gtcggcggca actcgttatg ggcaaacttt
     5041 tttgcttcca ttgtcggcgc ttttatgtac tttgccacac tgaccgaagt tcctatttta
     5101 cagggactca tcggttccgg tatgggccag ggaccggcgc tggctcttct tctggcagga
     5161 ccggcgcttt cattgcccag tatgatagcc atcgccggca tcatggggat aaaaaaaacg
     5221 tccatctatt gcatcattgt ggtcattctc tcaaccattg ccggcatgac ctatggatgg
     5281 atggttgcct gaaaaatccg caggcgatga acacgccaaa ttgtcgggac aaaagattaa
     5341 cgataaaata tgctcaacat atcttgaaaa atatttttct tgagctaaaa taaaacataa
     5401 ggtattgagg acatcaggca tgacaaatgt aaatgatttt attggaaaat atcggaatat
     5461 tattaccatt gcgttgtcgc tgatcggcat cgttctgatg gcctattatg attattgcga
     5521 tacagagtgc agctatctga gaggcgacct gctgggaatc gatctcaaat gggtcggtat
     5581 cgcttatatg gccatcatca taatcttcgc cgcttttaaa cagacacctt ttgtccgggc
     5641 tcttctggct gccggtcttg gcgtcgaggt tcatttatac gcttttcaga tacaaaataa
     5701 tgtctattgc ccgttttgtc ttgcattttc ggtaatgtta attttgtcat ttatcattaa
     5761 ctatgaagtt ccatccgcat ggcgggaaaa acgcggtcgg atgtggctgt attttctcgg
     5821 ggaggttgat tttccgatgt tcaaaattca taaattgccc ctgctgattt tcagtttact
     5881 gggatattta accgtttttt tgaccttcaa cggatccgtc acaccggctt atggtcaaac
     5941 gccgtccggt gccattcctt cgctgggcaa agggccctat gaaattatta tctttaccga
     6001 ctatttttgt ccgccctgtt atcgaatcga tacaaaagcc gagcccctgc tgaaagaatt
     6061 attggccacc cagcgggtaa aactgacatt tgttgatgtc ccctttaacc gatcgacacc
     6121 tgtttatgct aaatattatc tgtacgcggt gcaagcccat tccgatgcaa caagtgtttt
     6181 tcgagtccgt aagatccttt ttgatgcggc gcaatccaaa aaaattcaga aagaagcaga
     6241 tcttgtcgca tatttaaaaa accaaaaagt tgcgtggaaa acaatggatg aaaaatccgt
     6301 tttcccatcg ctgacggctg tgattcaaaa aaatgacatc cgggcgacac cgtcatgtgt
     6361 cataaaatat tcaaacaagg atagccaaaa gcttatcggt gatgtagaaa tatggaaggg
     6421 attgacggaa ttgaaggcac gcctatcagc cggaaacaag tgatgacctg agcgatgtcc
     6481 cgaagtccat ataaacaccc gatactcttt gccaagaccc aaatttaaaa aacattgtgc
     6541 cggaatcttt tccggcacaa tccaattcac gcattgtagg atcccgcacg gcgcaaaaga
     6601 tcatatgtgc gatttgcatg gggaatcaca ccgtgacggc gacaccttcc agcagtgcaa
     6661 atcaagcccc tgcgatacgc cgcaatttct tcttaaattg actgatctga tgtctgaacc
     6721 agacggctct ttcagtctcc ttcgattcca gcgcttttaa tcttgatgcc ttgcactctc
     6781 gtattttagc ttttaattcg ggtttggtca ttttgacttt gccgatatgt ttcttcttga
     6841 ccggctcgtc atctttaatc ccccgggtct cctttataaa ggcgatgagt tcctcttttt
     6901 tcatctcgtg aacagccttc tcatgaggaa tttcggacgc aatttcccga agttctttga
     6961 ccgtcatttt gtccaggtgc ttctcatccg gttctttatg actttttttg tggatttctt
     7021 ctgacatcgc gcatagacct ccttacattt tttatattca ggaatcaaat ttgcgatgaa
     7081 tgattgtgcc taccttatat cacaagtttt cacgtggttc aatccgcttc catatttctt
     7141 gacaatctca accgcaccgt gtaaggaaac gatacaccac atcaacatca ccgcataaga
     7201 agagcagaag aagggtatta aaaatacact acaggaggat ttttatgtct aaaagaaatg
     7261 tctgtattac cgtcgtgctg atgcttttga tgactgccgt aacctgtttt tccgctgata
     7321 aatatcaatg gaaactcgtt gacacaaagg acaattgcca aatctatacc agcgcagttc
     7381 ccggcaaaga ttacattgcc gccaaagcca catgccttat tcctgcacgc atagaagtga
     7441 tcggtacaat tttgagagac atccccaact atcccgaatg gatgcacgac tgcaaagcaa
     7501 caaaaatgtt gaaaacggtc gatgatgaaa aggatgtttt tatcttctgg ttccgtcagc
     7561 atattgatct gttgacagac cgggatatga ttctgaaaag taaaaccgtg attgatatgc
     7621 aaaacggaac caacctggtt tatgccgact ccacaaatga actgccctat gattccggca
     7681 agggatatgt ccggatgcct tcattttact cactgttcac tctccagtgg gtagaccggg
     7741 aacgcaccag ggtcacgttt atgatcgatc cgaatctcgg atccggcgtc ccatccggaa
     7801 ttgccaacag caaaatcaaa tcgacaccct ttaaaacttt acagaacatg atgaagatgg
     7861 tgaaaaaatc aaaatatata gaagcggcca aaacctccaa gtacaacaag atggccgagg
     7921 atttcgtaaa agccgggcat aaataaacat tgccgttcag cataaatcac ttaggaaagg
     7981 cggttcaaag atgatccgcc tttcctattt ttatggattg atatatagcg acagacagcg
     8041 ggaaaatctg ataataaccg ggtatttaaa acaacaaaaa aatggcggat tctttccgtt
     8101 atttttttga gtcaacgctt gacattctga ttttatcaat atataagaca caacttgctt
     8161 gaaacggctt taaggacgtc cccatcgtct agaggcctag gacacgggcc tttcacgcct
     8221 gtaaccgcgg ttcgaatccg cgtggggacg ccaatgtaga aataaaaagg gggaagtttt
     8281 ccagcttccc ccttttttct tgatttcaat ttcaacgcta ttcttctttt ttcttggttg
     8341 ctttcttttt cggagcggcg tcatcgcctt cgtctggctg cttctttgcc tttttcggtg
     8401 ccgcctcctg tccgtcagcg ggctgttttt tggccttctt ggcagcgcca tcttctgcag
     8461 caggctgctt tttgggttct tttgcggcac cgtcctcttt cttcttcgct ttcggctttg
     8521 cttcctcagc agacgccttg acttcttcaa cgacagcagc ctgtttggcc gcttttgcct
     8581 gggccaatgc ctgcgtctgt tggacaaatt tatcgaatgt tgcgattctt gccttggtct
     8641 gtttgatttt tgctttcagg cttttaacga ttgcgtcatt ctgggctttt tcagggctga
     8701 ttcccttctg ctcaagctgc tgcaaacgca cagccagttt cttttcgaaa atccggagtt
     8761 gttcaattct tacatctttg ctttttgatg ccatcttccc tctccttgtt tatttaggtg
     8821 gtttttaata acaatttttt ttatttaaat ctataaatat tattgtcatc gacgggccga
     8881 tttccccaaa aaagttacaa tgccatgatg cgttttaggt acccgatcac ttcaatatgt
     8941 tctgtctgcg gaaacatgtc caacggatga agactttgca aatgatatcc gccttcacac
     9001 aagtatttga tatcccgcgc ctgcgtagcc ggattacatg aaatataaat aatgttcgac
     9061 ggttgcaaat ccattagagt tttcaacaac gtcatttcac aaccgacacg cgggggatcc
     9121 aaaacgatca gatcaatttt actgcccaag gacaggaatt tttcccgcaa tacaatttca
     9181 acattcccgt caagaaaacg gacatttcgg gcattcagat ttgcagcatt tatttttgcg
     9241 tattttacgg attccttgtc catttctatc ccggtaatgt cctcggcaaa aggtgaaaga
     9301 aaaatggaga aaaggccgct tccgcaaaag gcgtcaattg cgttttctgc ttttccaggt
     9361 cttaccagtc gacaaacttc atccaccagc cgatccgtca gatattcatt tgcctggaaa
     9421 aacccgtcat caggcgccag aatccttttt ccctggacaa tacggacgac cgacgtctga
     9481 tcttcagagg taacatccgg atatcgggac cagattgtca gctcatcttt atgccacaga
     9541 ttttgtcgat tctcccgcaa gatactcaat tgctcattaa tggtttcctg catgattgcg
     9601 caatgctcaa tgtcgacaat ttttccgccg gatatatcca gaaatccgat gtgccctgtt
     9661 tggggatcgt ctcctacatg ataccgggcc ttgccgcgat aatgatacat tcgtggagaa
     9721 gctacggttt ccaaaacagg cggcgcgtca actttgccga ttctccgaaa agcatcttcc
     9781 acctgttttt tcttgatggc cagttgatac gcataatgga tgtgctgata acagcagccg
     9841 ccgcatacgc cataataacg gcagacggga tccgtccgcc agggcgacgg cttgatgatg
     9901 ttccggattc ggccgcgcag aaagttcttc ttccgttggg tgatttcgat ttccagttca
     9961 tcaccgggcg ctgaaaaagg aacaaaaacg acaaggttat ccgcacgacc gactcccgac
    10021 ccgccaaaag ccaccgattc aatttttatc acagatcgat ttttcatttc aaaacaatgc
    10081 tccgtgtctg acgtaacaca tcaataaatc aatcgtcaat aaatgatggt atttttcttt
    10141 acaactcata ataatgttgt taattaaaca cagtcctgaa acggttacag aacaattttt
    10201 tttaaaaaag gataaaaatg ccggaacgaa aatcaatact ctgtcccaat tgccgtaaat
    10261 tgatcagcat ggatgaaccc tcctgcccct attgcggact tcaacgtccc gggatgcaca
    10321 atatcgccgg caatattgga aaacttttta tggcgtccaa tccggtcatg atcatcattt
    10381 acgttaatgt tgctttgtat attttttcga tatttctgga cccgcagggc atttttggcg
    10441 gcagcggtcc tttcgggata ctggcaccgt ccaatcaaag tctttttctg ctgggtgcaa
    10501 gcggcacctt tcccgttatt ggagcccacc gtttttggac cctgatttcg gcttcttttc
    10561 tacatggggg aatttttcac attctgttca atatgatggc attgtatcag ctgggaccgt
    10621 ttattatccg ggaattcggg gttcaccggt ttatcaatct gtatatcgtt accggcatat
    10681 gtggattttc cgcttcagtg atttcaggaa cgccattcac gatcggagct tctgcagcga
    10741 tctgcggtct gatcggtgca atcatttact acgggaaaag ccgcggcgat tcctacggag
    10801 aagccattta caagcaggcc atgggctggg tcatcgccct ggtcattttc ggcctgattt
    10861 tcagaggtat cgacaactgg gctcatgccg gcggactgct ctcgggtatc ggtttttcgt
    10921 ttctgatggg gtacaatgac aataagcagg aaaccgcatg gaacaaaatg ctggcttacg
    10981 cctgtatcct gctcacggcc gcagttttat tatggtcggt tgtcaactcg cttttcatcg
    11041 gcctgaacat atccatctga acatgctggc ggctaatttt ttgaagacgg cttcgccata
    11101 tatctattca agaggagttt aattgaatgt cagagaaatc agattttcca gaaccgacat
    11161 tacgtgaagg agatgagctt tacggcttca cggttacacg cataaaaacc ttccccacga
    11221 ttcatacgac agcttatgaa atgaaacaca ataaaaccgg cgccaagcta ctgcatctgc
    11281 actgcttcga ccgcgaaaat ttatacgcga taggattccg cacgcctccg tacgattcca
    11341 ccggcctgcc gcatattctg gagcactcgg tgctggccgg ctctgaaaaa tatcccttaa
    11401 aagacgtttt caaggagtta atgcgctcaa ccatgcaaac attcatcaac gcgtttacct
    11461 atccggataa aaccgtttat ccggtagcca gtcagataaa aaccgatttt tacaatctgg
    11521 cccgcgttta taccgatctg gttctgcatc cgcggatgct cagggagact tttcttcagg
    11581 aaggccatca tctggaattt tctgttttgg atgatctgtc cagtgatctg atcatttccg
    11641 gaatagttta taatgaaatg aaaggcgcct attcctcccc cgattctctg atctataaga
    11701 cgcttcagga aaatgtctac cccgacagtg tttactcatt tgattccggt ggggatccga
    11761 acgttatccc ctcgctgacc tatgaacaat tttgcgaatt ccacagcaag tattactcac
    11821 cctccaatgc aaggtttttt atttacggtg atattcccac gaaggaccat ctggtgtttc
    11881 tttctgaaat actaaaggat ttcaattata tagaaataaa ttccgccatc ggaagccaga
    11941 agcgtttcac ccagccgcgt tcggttgtgg gagggtatcc catcggaaaa gatgaatctg
    12001 ttgaacgtaa aaccgttgtt aacatcgctt ggctgatgtc tgaaaatacc gattttgaga
    12061 cctcattgat cctgcaaatc atatccgctc ttctcgttgg cagcgcggcc agtccgctgc
    12121 gcaaagcgct gattgactcc ggtctgggag aggatctgac tccggtgagc ggcgtcgaag
    12181 cagacctgaa acaattaatg ttctgtgtcg ggttgagaaa cacacagcgg tcggacgcac
    12241 aaaaaatcga acaactgatt ctaaagactt taaaagacat tattgattcc ggttacgaca
    12301 cgcaactcat cgaaggcgtt ctgcatcagg tcgaatttca cggcaaggaa attgttcgcg
    12361 gttcttatcc ttacggaatt tctctgatgg gtgcggtttt tcaaacatgg ctttatgacg
    12421 gcgatccgtt aatcggactg gatttttcac aaatcataga gaatatccgc cgctgctggg
    12481 ctgatgatcc cctgattttc cagaaaatga ctgaaaaatg gcttttgaat aatccccatc
    12541 gcgttctggc cgttatggaa cccgatcccg atttcaacga aaaaaaagag gctgcttaca
    12601 gagaaaaaat ggacacgctc aaggcatctc tttgtgaaga agaattaaga cagattaatg
    12661 caaaagccaa gaagctcaag gaatttcaaa atcaacagga tccgccggaa gcggcctcag
    12721 ccctgcccag gctgaacatg gaggatattc cgaaatccat tgaaaaaacg ccgacccgaa
    12781 attctttgat cggtgacgtt ccggtctggg agcacgatct gtttaccaac ggaattgcct
    12841 atgttgatct ggcttttgat ctggcagata tccctgaaga gctgcagatc tatctgccgc
    12901 ttttgggaaa gatgatgacc ggcatgggcg ctgccggttt gacctatgag gaaatggcca
    12961 aacggattgc tctgaagatg ggcgggttgg gctataatct gggcgcagga tttaccgcaa
    13021 atggcgactc aacatggcag aagatgattt tctcgtttaa ggcgctatat cgcaatcttc
    13081 cggaagccat gagcatcatc agcgatgtgc tctgccgcgg tgatttaagt cacgaagccc
    13141 ggatgcggga tctgatttca gaaagaaaaa acaatcttca atcagcgatt cttccctccg
    13201 gtcatctatt cgccagaatg gcagccggcg cggcactcac cctcccggct taccgggatg
    13261 aacaatggca cggacgaacg caatttaaat tcgttcaagc aacagcgggt gagttcgttt
    13321 catcaaaaag tgccttgcgc gataaactgg aaactttaaa gcaaaccctg ttcaataaaa
    13381 acaacatggt cattaatgtc accgccgagc atcaggggtt ggaaattgcc aaagaacacc
    13441 tgccgtgtct actcgacagg cttccggcgt cttcacaggc cgctccggaa gaagaacctt
    13501 ctttgaatcc ggtatttgcg ggaatctctg tccccgcgca ggtatcgtat gttgcctaca
    13561 ttttaaaaac gccttgttat accgacccgg caagccctct tttgatgatt gccgcaaaag
    13621 agttatccaa taactatctg tacaagtata tccgcgttca gggaggcgct tacggcggca
    13681 tgtcttcttt cgacgcttcc atgggattgt tttcgttttt gtcctatcgc gacccgcgca
    13741 tttccgagac gcttcaggtg tttaaaaacg cacagacgtt ttacagtcag aatgaaattt
    13801 cttcaccgga catggaaaag gcgatcatca gcaccatcag catgattgac aaaccttcgg
    13861 atccggccag caaagggtat acggcaatga tgcgcaattt tgccggaata accgataaga
    13921 ttcgccagaa attcagagac gacattttat ccgccacacc gcaaaaactg aaaaaagtat
    13981 tatccggtta tttctctcag gcgttcaaat cagcgtcagt ggccgtttat tcctccccgg
    14041 aaaaactgga cgaagccaac agacaattgg aagacaaact gcatattgaa agtatttttg
    14101 aaaattaatc caatagctct tcataaaaaa agaggctaaa tatactttag cctctttcat
    14161 gttcttctgg gattttaaat cgcaatcgca acaaaatcct tatcgcattt ttttattgct
    14221 tttctgctac attgattctc gcgatggctc tcgccagtgc cgcccgcata atttcaaatt
    14281 ccacgtcatc tttgttcatc tgagataaac ggctttcagc attatctttg gccttaaggg
    14341 cccgttcttt atcaatctga tctgattttt cagcggtttc aatcagaacg gtgactttat
    14401 cgcctgtaac ttccacataa ccggtattca ccgcataata cgactttttg ccgtccttga
    14461 cataattcat ctcgccgatt tccacggacg ttaaaaaagc ctcatgaccg cgcaatacgc
    14521 cgaactcgcc ttccgttccc ggaagggtaa cttcttcgat attgcccgaa aaaatcatct
    14581 tttccggcgt tacaatttcc atcaataatt catctgccat ttacattctc cagatcaata
    14641 attattcgcc gccaatcttt ttggcttttt caacggcttc ttcaatgccg ccgaccatgt
    14701 aaaaggcctg ttcgggcaga tcgtcatgtt tgccttccag gatttctttg aagcctctga
    14761 tggtatcggg cacggaaaca aacttgcctt ccgtaccggt gaactgagcg gcgacgaaga
    14821 acggctggga caggaatctc tgaatctttc tggcgcggct aaccgtcagt ttatcttcct
    14881 cagaaagttc atccataccc aaaatggcga taatgtcctg aaggtcctta tatttctgca
    14941 gcgtcacctg tacctgacgt gctaccttgt aatgttcctc acccagaatg ttgggatcaa
    15001 gaatacggga tgttgaatcc agaggatcca ccgcgggata gataccgagt tccgcgatcg
    15061 gacgggacaa aacgacggtt ccgtcaagat gcgcgaatgt cgtcgcggga gcggggtcgg
    15121 tcaagtcgtc ggcaggcacg tacacgcact gaacggctgt aatagaacct ttggttgtcg
    15181 aggtaatccg ttcctgaagt ccgccgaggt ccgtggccag tgtcggctga taaccgaccg
    15241 ccgaaggcat acgtcccagc agagccgaaa cttctgaacc ggcctgggtg aaacggaaga
    15301 tattatcaac aaagaagagc acgtcctgac cttccacatc acggaaatat tcggcagccg
    15361 ccaatccggt caaagcaaca cgggaacggg ctccgggggg ttccgtcatc tggccgtaaa
    15421 tcagagcggc ctgtttaata acgccggatt ccagcatttc gcggtacagg tcgttacctt
    15481 cacgggttct ttcacccacg ccgcagaaca cggaaatacc gccgtggtgc atggcgatgt
    15541 tgttaatcat ttccatcata acgacggtct tgccgacacc ggcgccgccg aacatgccca
    15601 ttttaccgcc gcggggaaac ggcaccagca gatcgataac cttgacgccc gtttccagaa
    15661 cgtgcacgga tgtatcctgc tccaggaatg tgggcgattc ccggtgaatc ggcattttcg
    15721 tctgcgcgtt aaccggcccg agaccatcca cgggacgacc gacgacgttc agaattctgc
    15781 ccaagccctc tttaccgacc ggcaccatga taggagcacc ggtatctttt gctttcatac
    15841 cacgaaccaa accgtcggta atatccatgg cgatgcagcg gacaacattg tcacctaaat
    15901 gcaacgcaac ttcaacgacc aaattatctt cctgatcatt gatggcggaa ttggtaatgg
    15961 taattgcgtt taaaattgcg gggagtttgc cttcctcaaa agccacgtca actacaggtc
    16021 caataacttg aacgattttt ccgatattca taactatctc cttgcaaata gtcgtattaa
    16081 tttattttac atgcgaccac agccgcagaa aaattaatat ttagcctttc gccagagcct
    16141 ctgtaccgcc cacgatatcc atcaactccg ccgtgatcgc ggcctgtctg gctttattca
    16201 ttttcaacgt taatgaactg atcaaatcct ggcagttact tgtcgcatta tccatagccg
    16261 ccattcgcgc gccgttttca ccggctgacg tttccagcag agcgcgataa acaagtacgt
    16321 gcacgtacat cgggagtaat ttatgcagta agacctcttc cgatggttca taggaataat
    16381 caacccgttt atccggttca tcctcaattt cctggccgat ggatggcagg gggaataatc
    16441 tgaccacaac cggtttctga atggaaacat tcataaactg attataaatc aaataaagtt
    16501 cgtcatactc ttctttaata aaaggattaa tgacttcatc ggcgatggaa accgccagcg
    16561 tcatgtcaaa tttactcaaa acatccgtct tctgggcaat gatgttggct tttttgcgga
    16621 aataatccct gcccttgcgc cccacggtaa tcatggaaac atctttccct tcgcccgctt
    16681 tttctttcag aaatctttca gtcgctttga tcaggttggt attaaaccct ccgcaaaggc
    16741 cgcgatccga tgtcatgcaa atcacacgaa ttcttttcgg gtcgcgcacg gccagtaatg
    16801 gatgtgacat gctttctacg cgcagggcaa gactgttaag cacatccatg aattttccgg
    16861 catagggacg gaaattttcc attttcaact gcgccgactt aaattttgat gcggcaacca
    16921 tgttcatggc gcgcgtaatc tgttttgtct tttgaacggc gccgactttt cttttaatgt
    16981 cttttagcga ggccacctac aattctcctc aacttattat ttgaacttca ggactgtcta
    17041 tttcctgaca aaaccgttaa tatctctacc tattccgcca caaaaacaga atcaaaagcg
    17101 gtcataattt ccttcatctt cttttcgagt tcgggagaga ttatcttctt atcttctatt
    17161 tctttcataa tgtcgggatg tttgctttca acaaaactca aaagctgctc ttcgtatata
    17221 cgaacctttt cgacatcgta tttatcaatg aaacccctgg ttcccgcgaa gagaacaacg
    17281 atttcctgtc ctaaagacat cggcttgaac tggggttgtt tgagcagttc caccaggcgg
    17341 acaccgcgat ccaactgagc ctgagtcgct ttatccagat cggatccgaa ctgggcaaaa
    17401 gccgccagtt cacggtactg agccaagtcg agtttcagcg ttccagcaac ctgcttcatc
    17461 gcctttacct gagccgcgcc gccgacgcgc gaaacggaga gaccgacgtt aatggccggg
    17521 cgaataccgg agaagaacaa actcggctcc agataaacct gaccgtccgt aatcgaaata
    17581 acgttggtcg gaatgtaagc ggaaacgtca ccagcctgag tttcaatgac cggaagggcc
    17641 gtcaaagaac caccgcctaa ttcctggctg acacgcgcgg cacgctcgag caaacgggag
    17701 tgattgtaga aaatgtcacc gggataagct tcacgtccgg gaggacgacg cagcagcaga
    17761 gaaatctgac ggtaagcgac cgcctgcttg gacaaatcat cgtaaataat caaagcatcc
    17821 tgaccgttgt ctcggaagta ttcaccgata ctgcaaccgg cataagcggc aatgtattga
    17881 agcgtcgcgg gatcggatgc acagccggca acaacgcagg tataagatag cgcatcatgc
    17941 tgcttgagtc tctccacaat ttgagacact gtagattttt tctgaccgat ggcaacatag
    18001 atacatttca caccggtatc tttctgacgg ataatggcgt caataccgat ggctgttttg
    18061 cctatctggc ggtcgccaat gatcagttcg cgctgaccgc gtccgatcgg cgtcatcgca
    18121 tcgatcgatt tcaaacctgt gtacatcggc tgattaaccg gctggcgctg aataacaccg
    18181 ggggccacca tttcaattcg gcgaaactct ttggtttcaa tcggcccctt gccatccaag
    18241 ggttttccgg ttgcatcaat tacacgtccc agcagtgctt caccgacggg aacctgtgca
    18301 attttaccgg ttcgctttac gatgtcaccc tctttaatgt aggtaacttc accaagaacc
    18361 gcgacgccga cattgtccgc ttccagatta aggaccatac ctaagattcc gccgggaaat
    18421 tccaaaagct ccatcgccat ggcattttga acgccgtata cgcgggcaat accgtcaccg
    18481 accgacaaga ccgttccggt ttcgcttata tccagctttt tctcatagct tttgatctgc
    18541 tcagaaataa tttgactaat ttcttccgct ttaattgttt ccatgtccta tcttgcctcc
    18601 cctaagagat tcctcatatt gttcaactga ttttttatac tgccatcgta aagtgtatcg
    18661 ccaacgccga tgaccacacc acccaaaagg gtggggtcct gttccacagt catatcaact
    18721 gttttaccca gcgctttttc caggtttgca ccgattaaag aacgtaattc atcggaaaga
    18781 gcgaaagcgg tttttacatt cacccgcacc ttcttcagcg cttcgtccat taactggcga
    18841 taacaaacga cgatatcctc taaaatatcg attcgattct tgtccaccag cagcctgata
    18901 aagttggccg tcatgccgga caacttcaac ttggcaatga gattgtccac aacacctttt
    18961 ttaactgcct gttcaaagac aggatttgcc aggaaatctt ttaaagattc attccgagaa
    19021 atgagtaatg tgaactgatc gagttccttg taataaggct caatctggtt ttcctctttc
    19081 gcaatgtcaa aaaaggcacg cgcatatctt ttggaaatat tgctgatcaa tgcttgatta
    19141 ccaccttatt tacgtattct ttcaccatgg ctgcgtgatc atcctgtgta atgcttttct
    19201 tcagaatttc ttccgccatt tgcacagaaa gttcaacagc ctgggatttc agctgttctg
    19261 tagctttttt catttcctgc tcgatgcggg cgtgagcatc ttctttcatc tttttggctg
    19321 ttctctcggc atcctcgata atcttctgtt tctccgtaac gccctgcgcc ttgatcatct
    19381 caatgatacc gtctatttct gttgacgcct tgtcaatttt ttccgaatat tcacgatact
    19441 ttttctcggc ttcggctttt ctctccaccg ccttctccag attttcctta atctccgccc
    19501 ggcgaccgac gaaaaattct ttcaccttgg cagccagaag ccaatagaga agacccatta
    19561 aaacgacaaa gttgaaagcc ttcttgaaca aaccgaacca gtctgcaccg tggtgccccc
    19621 cttcagcctc agcggaagca aatgcaatac ccacagcaac cagcaataca aacacacccg
    19681 ttatcagaga tattgttttt ttcattgtac cggcctcccc aatattttct gggcgatctc
    19741 aagggataac tgacgggaat tgccgtccag aaccgctttt gccttttcca cttccttatc
    19801 catcttctgc tggaactcgc gggccattac cggaatttca ttgcgcacgg catcaataat
    19861 attttttgcc tgattcgcac cttcctgtat gacctctttt ttagcctcgg acgctttgag
    19921 cttggcctgc tttaattttt cctcatactc tgcaactttt ttctcggcgt tttcatcaaa
    19981 aagcctgatc tcgttctctg attcttcaag ctgttttttg cgccggtcaa taatggaaag
    20041 gatgggtttg taaagaagga tgttgagaaa aaaaactaag gccagaaaaa gaaccatttg
    20101 aactaaaagc gtataatcgg gaatgactgt aaccattttt tacctctgct tatctcttgc
    20161 actttctata aaaaccgcag gaagattttc ctttatggac ggttttcgag ggcatgccta
    20221 agcaagtgca tacagttttt attcagttgt gttttgaata tttttaccta aaaaggatgg
    20281 aatctaattt ccagatactc aaaacctgcg agttactaat cacatcatta tgggcttgtc
    20341 aagtattttc tgaccatcag tctgtttttc tgcaataatt aataatatca aagacttggg
    20401 gaattaaaat gctatcaggc tgttttttat gcgtttgacg tcaagaccca aataatactg
    20461 atggtttgca caaaatcaat ccgccttggg tatttcaatt ttctgctgcg caacgtcatc
    20521 catatgggac atcccattaa agatggcgcc cgcttccata acaaacacag gggttcgcac
    20581 atctccggaa aatttccctg ttgagtgaat attgattttt tcttttacat cgatgttgcc
    20641 ctgcacgtcg ccgctgatat aaacactttt ggtatcgatg ttggccttga cggttgcgcc
    20701 ctgaccgaca accagtgatc cctgtccgaa aatttctcct tcgaattttc cgtctatgcg
    20761 aacggaaccg ttaaaaataa gtttccctga aaactcggct ccctgaccga ggaaggcttt
    20821 tatttcttca gcatctttct ttttttccca cacatcgtcc cccttttgat acttatgtta
    20881 atcgattcgg ctcaaaatga ttttctcttt atactcgatg actccttact tttgcaggat
    20941 aaaccggcgc gcgctgacat tgttcagcaa tccgatggcg gccatcagcg aaagcatggc
    21001 ggatccccca taactgaaaa aaggaagggg aataccgaca acgggcagga tgcccagcac
    21061 cattcctata ttgataaaga cttcccacgc aatcagcgcg ctgacaccga acgctataat
    21121 tgtgcctaat aaatctctgg cttgcaaggc aatttttaat ccccagaata tgatcaccac
    21181 aaacaggcct atcagcaaca tggaaccgat gaatccccac tcctcggcaa aaacggaaaa
    21241 aacaaaatcg gtctgctgct cgggaaggaa cttcaactgg gtttgggaac cgcttaaaaa
    21301 acccttgccg aaaatttctc cggagccaat ggcaattttc gattggatga tatgataacc
    21361 ggagcccagt ggatctcttt ccggattgag aaacgtcaca agcctttccc gttgatagtc
    21421 cttgaggaaa aaccagccag ccggcattaa gaccaaaccg gcggcaaaaa caaaaatcag
    21481 agatttctta tccacaccca tgaaaaacac tatggaagca aagatgacca tcagcatcag
    21541 agccgttccc agatccggct gttttaaaat cagcacgaaa ggaacagcga caatcaagag
    21601 cggaatgaaa agctccttta acgtgtaagg ctcgctggat ttgtggttgt cgaaataacg
    21661 cgccagggta aggatgatga ccactttcat caactcggaa ggctgaatgg caaagaaacc
    21721 gccgcctagc cacctctggg cgccatgcga cgacgaaccg acgattaaaa ccagaaccag
    21781 gagaaaaatg gaaaaaccgt aaatgatgta ggcatactcg acaattaccc ggtaatccat
    21841 gaaaaacaca aacgtcatga aaagcaatcc cactacaatc cattgcagtt gcttcaggta
    21901 aaaaggcgac tggcctcctg ctgcactgaa accggtggag tagatattca aaactcccat
    21961 cacgcagatg ccgagcacaa aagacaacag aatccagtca aaataatgaa aaatacggcg
    22021 atcgaattta agaaaaatca tctgtcgtcc ccatcaagat ttctgcggtt gtcagattcg
    22081 ccgggctttt cactcttccc ggcgcttttt tcttttttct tcccttcaaa ataagcggat
    22141 aatactctgc gcgcgaccgg tgcggcaacg gctccgccgc ctccggcatt ttcgaggatg
    22201 acggccacaa cgatttccgg acttttcgac ggcgcataac tggcgaacag cgcatggtct
    22261 ttgtgaaagg cgctcaggac cttgcggcgt cgcgcctttt cattttccgg caatcccaac
    22321 acctgggatg tccctgtttt accgcatacc tctatctgtg ccagtttcgc attctgcccc
    22381 gtgcctccgg gctcattgac cacggccttt aaagcatcat ttataatttc catcgtggtg
    22441 ttgctcagtt tcaactcccc ctttttcacg ggcgggaaat ccttataggt tttaccgtcg
    22501 ggagcatcta ttttcttgac cagatacgga cgccagaggg tgcctccatt ggcaaaagca
    22561 gcgtaggcat tggccagttg taaagttgta accagattaa acccctgtcc gatggatatc
    22621 gatatggttt cgcccatctg ccaggcctct tttttctttt tcaacttcca tgccttggtc
    22681 ggcaccagtc ctttcttctc attaggtaaa tcgacacccg ccagatctcc cagaccgaac
    22741 cgttttgcgt attcggctat tttgtccacg cccagcattt tgcccacggt ataaaagtaa
    22801 acatcacatg atccgaccat ggcctgatga agattgacat gaccgtgacc gccctttttc
    22861 cagcagcgat atgttctgtt tcccagagta aaaaagccgt tgcagaaaat tttcgtctcg
    22921 ggcgagataa taccttcttc caaagcagct gcggcaacaa ttaatttata ggttgatccc
    22981 ggagggtatt gtccggagat cgctttattg gacaacgggg caaacggatt gctctgcaac
    23041 tgtttccaga gttcgctgga aataccttca ttaaataaat ttggatcaaa cgaaggggaa
    23101 ctcaccatgg ccaggatggc gccatcgcgc ggatccagtg cgacaacgga acccgctttc
    23161 ccttcaatgg cctgccatgc cgctctttgc aagtccgcat cgattgtcag agtcatgtta
    23221 tagccggaga cgggatcgat gcggcccagg ttttttattt ctctcccgta aacattgact
    23281 tcaaccagtt ctgcaccgcg gcggccgcga atatattcgt cgaaaaatcg ttccaaacca
    23341 tgttttccgg atatatcgcc gtaagcatac ttgccctcgc ttttttccat ttcttccctg
    23401 ctgatttcac cggtgtaacc gatgatcggc gccaggattt ctccgtcaag atacaaacga
    23461 atcggcgaga catcaatgta aatcccgggc agatttaacg cgttggtttc caccagggct
    23521 attttttcca tactgacgtt cttgtccaac tttacaggca aatacggttt tgcgtttttc
    23581 agaacggctt gatcaaacgg gaaaaccatc gattgacttt cataaagcga ttttaatttc
    23641 tccactgaca attcattggc cttgtttttg ttgggaatat aaatcacgtc gaaagacggt
    23701 ttgttgtcga ccagaacatt gccgttacgg tccatgatca gaccccgtag cggcttaata
    23761 aaccgaaaac gcacggagtt attttccgat ttctttttga attcttctcc tttgataatc
    23821 tgcaaatatc ccaagcgacc gatcaggata cagacggcaa gcagaaatat ccccgttatg
    23881 atcctgattt tccccttgaa ttcaatcggt tcgtgtccgt taagcatatc atgccttctt
    23941 ttcgtagagg aatattccag ttctgtcgat aagataaaac accgcgggag cgaccagtcc
    24001 gacaacaaat gactgtatca gaatcagaga aaatatatca gggcgcatcc tgatatcaaa
    24061 gaccagaaaa taaaaggtct ggagcatcgt ctctttcagg aaaacacaaa tgaaactgaa
    24121 taaaacaatg acatgatttt tcccggtatc aagcaaatcg gatatgaaaa atgaacacag
    24181 gaaaatcatg atatacgcca aagcaagcac cccgggaacc gaaccgccga ttaaatccag
    24241 aataaatccg aggtaaaagg ccagcaacaa ccctttgatc atttcaaaac ggaatcctgc
    24301 gtaaatgata atgatcagag atacttcaaa aacaaaagaa tttgaaaaca caatatcggt
    24361 aacagtggac tgcagcacga taagcagtag ggataaaatc agaagcagca aataatgaaa
    24421 catgattatt caacggccgt ttcagcggaa gataaaatta ataattcctc cagcttggaa
    24481 aaatccgtaa acggagcaac atcaattttt aaaaacaggc catcattctg ccggtccaca
    24541 ttgatgacct ggccgatcaa ccatcctttg ggaaaaacac ctcccatgcc tgaagaaaca
    24601 atgatatccc ctttccggac atcctgtgtc ttgggtatat actttaatat cattccccgg
    24661 ggaccggcgc cactgataat tccctgaatg cgatcccgct gcacgatagc gtcaacattg
    24721 ctgttttcgt cgataaacaa aagaacccgg gagacattcc gggagacatt tgtaagacgg
    24781 ccgatcagcc ctggataggc aacgaccggc ataccgtttt tcaaaccgtc ggatgcacct
    24841 ttattgatca gaacagttct ggacagcgtg gcctgctctc tgccgatcac cctggcggca
    24901 atgaaaggat atcggtgata atcgggaatt gctaaaagct tcttcaggcg ctgcgcttcc
    24961 agataacctt cctgatagga aacaataacc gcctggagtt catccatctt tttttttaat
    25021 tttttatttt cttcctgaac gccgactagc aagaaatagt gaagccatgc atccttgatg
    25081 ccctgaatgg aaccgttgat gattttatac accggagaag atatttccag cgcaactttt
    25141 cttaaaaaac ttttgtcatc gccgaatttc agatggtatg aaatcacaac caggactaaa
    25201 accataagga cggcaatgat aatgatgacc ttgtattttt ttaaaaaaac catttttaac
    25261 ctgacgggga aatgattaca aaacgcattt tatgtcaata tgccgggacg caagactgac
    25321 aaaatttctt cctttcgcaa aagatcatga aagaaattta tcttgctgtt ttttcacttc
    25381 atcgtaaaaa tcactcaaaa ccgccgggaa acgattccgt aataaaaaac tgcacacaga
    25441 ttaaaggtct gatttttaaa cctcaagggc gatgtccttg agaacattta tttgttcaag
    25501 ggccatgccc gcacctctgg ccacagccga cagcggatca tccgtaatgg tgatgggcag
    25561 tcccgtttct tcccggatga ggatgtcaat attggcgagc aaagctccgc ctccggtcag
    25621 gacgataccg cggtcaacaa tatcaccggc aagctccgga ggcgaattct ccaacgtatc
    25681 tttaatggca tcgacaatga aggtcacctg ttcgttgatg gcttcccgta tttcttccga
    25741 attcgtttcc gtaatcttgg gaatgcctga aatcagatcc cgccctttga tggacccgac
    25801 tttaatctca tcaaacggat acgcacagcc gatttccatt ttaatgattt ccgccgttcg
    25861 ttcaccgatc agcagattat gttttcgttt catatattga acaatcgcct cgtcgatttt
    25921 atccccggcg actctcacgg atttcgaata aacgattccg gccagggaaa tcaccgcgac
    25981 ttccgtcgtc ccgccgccga tgtcgacaac catcgaactg gtcggttcgg caacaggcaa
    26041 ccccgctccg atagccgcag ccatcggctc ttcaatcaga taaatttccc gggcccctgc
    26101 ggattcgacc gtctcgcgca ccgcgcgcct ctccacctgc gtgatccccg aaggcacgga
    26161 aataatgatc cggggcctca cgagtgattt gcggttgtgg acgctcagaa taaaatgttt
    26221 cagcatcgct tccgtaatgt caaaatcggc aatgacgccg tcgcgcatcg gtctgatggc
    26281 cacaatattt ccgggtgtcc gccccagcat attttttgct tcatttccca ccgccagcac
    26341 ttttttcatc ccccgggcat cctgatgaac agccaccacg gagggttcat tcaagacaat
    26401 tcccttatcc ttgacgtata caatcgtatt cgccgtacca agatcaatcg ccagatcgtt
    26461 ggaaaatttt ccaagcacat aatcaaaaaa cattgggcct cctgtaattt acaaattaat
    26521 ataatgactt tcaggcttta aatttttttc atacagtatt tcacggacct aatcaactca
    26581 tttttaaatt atatttgcat tttgtaaatg actataataa taagacaaaa attaatttcc
    26641 gaatgaataa atcctgtatc gttttgaggt gatttttatg ttgaaatttt ttcgcaaaca
    26701 cgcccgcggc tggttcatga tcgctgtgat cgcaatcatt atcgtggttt tcgttcttta
    26761 cttcggcgcc agcagaggag gcgaacacgc ccaggtggtt gccacagtgg acaaaaaagc
    26821 catcacggtc aatgaagtac tcaacgaata cgacaaactc ctggaagtgg ccaggctcaa
    26881 cctgaaagag aatctgacac cggaactgat caaaaaaatg gatctaaaaa cgaaagccta
    26941 taatgacatc ctgaacagag aagtgattat cgccaaagcg gctgatttaa agattcaggt
    27001 ttccgatgat gaactgcgca attccatcct gtccatgccg gccctgcaga caaacggcat
    27061 gtttgacgaa cgcaaatacc atcagattct acgcttcaac aggctatccg ccgaagattt
    27121 tgaaacgctg cagagagccg atttgacggc ccgcaagatt gaatttcttg tgcgggaagg
    27181 cgtaaaaatt tccgataaag aaatttacga cttatacgct ctgcagaacc ggaaaataaa
    27241 cgtggacttt ttgcgcctgg ctgccagtga tgtaaagcat aaaatttcgc cggttgaatc
    27301 ggaactggaa gattatctga aacgaaacgg caatcttttt cgcgtccccg aacagacaaa
    27361 aatcaaatac ctgtttttcg gcgctgacac ttttccggcc gatatcagcg atgccgatat
    27421 tcaggattat tattcaacgt atcaaaataa gtacaaagcg aaagacggca agccattgcc
    27481 actggccggg gtccggaatg atatcatcaa cgagttgaag agagcaaagg gcatgcaaac
    27541 tgcctatacc gaagccagaa aagcccgcga ggaaatctat caggacgaca atatggacgc
    27601 ctacgcccgg aaacacaatc tggcagtacg tgattccgat tttttcatga tcaccaaacc
    27661 gccgcaggac ttcgcttccg tcaagaattt cgccgatgcg ctgctgaaca tgcaaaaagg
    27721 cgaactgagc aaaattctct cgaccgaaaa cggctattat ctcttgcagg tcgtcgacaa
    27781 gaaagcttcc tacattccga aactcgacac gatcgccaat gaggtccggc aacgatttat
    27841 cgaaacagaa acacagcttc ttgccgaaaa agaagctaaa tcgattctgg aaagcctgaa
    27901 agccggcgaa agcatggaaa aagtggcgtc cgccaaagga ttaaagatcg gtcagaccgg
    27961 attttttcag cccggcaata ccatccccgg cgtgggagaa agtcctgaag ccgccgagat
    28021 tctgatgacc ctctccgcgg ccaaccctta cgccgaaaaa cctcttttca tcaataatgt
    28081 ctactttata ttaaaattca gggagacaac cagaatcgac gataatgctt tcactgcgga
    28141 aaaagaaatt tacagaaaaa tattaacggc gatgaaacag gaagaagcca tgcagacctg
    28201 gctggaagga aacaaagcgg acatgatcaa ggaaaagcgg atcaagatta aaaagaaggc
    28261 ggaagattta taattacata aaatcacgtg tcgttcggcg cacaataccg tccgggacac
    28321 gtgaacgcgg ccatactata ctttctcttt aatgtgagcg ttttgtgggc cagcgcaacc
    28381 atcaaacatt tcgcttttga aaattgcaac tctttaaaaa acagaaggga ggggacaaac
    28441 acccctccct ttaaattttg gtggagctga ggaggatcga actcctgacc tcttgaatgc
    28501 cattcaagcg ctctcccagc tgagctacaa ccccaataga ttccccgaga attcgcctgc
    28561 cccttaacat gcccctcccc ggctgtcaac agtttttcag cttgactttt ggccttccgt
    28621 ctatataatc gcgcccgtgc cggagtggtg aaactggtag acgcagggga ctcaaaatcc
    28681 cccgttcgca aggacatctc ggttcgattc cgagctccgg caccagtata aaaacatata
    28741 tatatcaatt agtaagaaag aataatgtat aaaagctaga caataaatgt ctagctttta
    28801 tttttgatat aactaaaaat tgccactatg ttgccactct agaaaaattt gataaatttc
    28861 ctaaaataat gaaatcgcgt tgctgttttg gatatatata ttgattcagc tacgaaatta
    28921 taatgatagc ctaaagcatt gaacctctat ttatattata atttaccgca taccccccaa
    28981 tataccccca tatttactca ttaagttaaa ttagattaag cggtttttat cagtcatatt
    29041 attgacgcga aagacttctt ccacctcttc aagtgagtat tcaggtggta atgactcaca
    29101 tgccggatca aaaagcatta tacctccttc atcttccgtc aaaacagaat agagaccact
    29161 tgcagccatt attttacctt ctgttatgtc tgagcgaatc ttttgctcat cctcttgact
    29221 ctcaggatta aattccattg ataatttaaa tacaaaaccg ctgctatcta gagccaagca
    29281 atcaactgaa atctgtcttg aattgtctgg atgtaaaaat gactcaacat gcagaatctt
    29341 aactattgta tttaatgcac agttgcaggg gaaaaaatga aaaggtacta tgtcatgtgt
    29401 ttcccccgtc tccatattct ctattgttcc tgttattttc ttatccttca ttttataacc
    29461 tctttattca atgacttata tatcttatct ataatatcga ccatacccat agtatctagg
    29521 ccgcaatatt cttccagatg tctccttact tttgcattat ctttattatc ctctgaaata
    29581 taaccctgca atatttagca ctggctgtac cgccatcccc tcttcctata tcattatccg
    29641 actaataaat ttgcggttct gttcttaaac gataaagctg cacttttatt gttagaagat
    29701 gaaatatcat ctatatcaaa ctgccatgct ttttgattaa ttcttgctgt tccggttttc
    29761 cttttttgta ggcattccat ttatcttcaa actcggcatc cagcaaattt ttgtattcat
    29821 taactgattt gtcaattttc tttattccct ttgtttctat aaattgtttc agatttgtcc
    29881 gtgaaaccat tggaagaggt atactcgtgt caaatgtaga gatttttttg gaacgacaat
    29941 ccacacacaa aatagttgct tttttctcgc cctctgtgat ctccacaaga tgaaggtcat
    30001 taattgaatg gcagcttgca catgaaggga acataccttc gggatcagga tcatttgaaa
    30061 aatcaaacac cgtttgacga acatcaacat cggcaccggg gccttctcca tgggtgtaaa
    30121 cttcaccgga acgataaaga tccagtagga gagcacgctt aaacttctgt cctggtccaa
    30181 tgcccgcgta ccgcaatctt cgcagacctt cctcgcgaag ctgaaaagtt acgatgctat
    30241 tgcgtatttt tgtaagaatg taacagtcat cactgtcttt gtattgatgt aagactggca
    30301 tttaattgag atataaacaa aagttgtgtt taggaagata ggtggtgtat ccaccggttg
    30361 gt
//
LOCUS       DAOOLB010000022        28035 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_1493_length_28035_cov_44.120586, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000022 DAOOLB010000000
VERSION     DAOOLB010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 28035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28035
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1493_length_28035_cov_44.120586"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="PLF77_04600"
     CDS             <1..329
                     /locus_tag="PLF77_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPC08166.1"
                     /translation="AVRDEGVQSGVCLVYTPHTTAAITINENADPDVPRDILAALDRA
                     VPLSANYRHAEGNSAAHVKSSLVGASELVIIENGHLVLGTWQSVFFCEFDGPRTRKFM
                     VKVIPD"
     gene            complement(350..793)
                     /locus_tag="PLF77_04605"
     CDS             complement(350..793)
                     /locus_tag="PLF77_04605"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPC08167.1"
                     /translation="MNGIKIFTIGFTKKTAEEFFSRLIRAGVKRVIDIRLNNVSQLAG
                     FAKRDDLCYFLWTIGKMDYVHRPDLAPTQQILDNFKKNKGSWEIYEKDFLALLASRKV
                     ETEIKPELLHEACLLCSEEKPTQCHRRLVVEYFQSKWENVRIIHL"
     gene            complement(790..1386)
                     /locus_tag="PLF77_04610"
     CDS             complement(790..1386)
                     /locus_tag="PLF77_04610"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HPC08168.1"
                     /translation="MKELFTVGHSVHTVEEFLEMLKLHAINALCDVRSSPYSRYSPQF
                     NRESLKEDVLKQGIAYIYLGAELGPRSSDQSCYENGKVQYKRLAEKEIFQQGLARLRK
                     GLETYRIALMCAEKDPLTCHRMLLVCRNLREENIHIQHILEDGSLEDNRDTEMRLMKM
                     LKIDPADLFSTEEEQIQRAYDLQGNKVAYTLEERQETL"
     gene            complement(1383..4262)
                     /gene="recQ"
                     /locus_tag="PLF77_04615"
     CDS             complement(1383..4262)
                     /gene="recQ"
                     /locus_tag="PLF77_04615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HPC08169.1"
                     /translation="MNRAQSILKNTFGFDTFLPLQEKIIAHILNKRDALIVMPTGGGK
                     SLCYQLPSIIFDGLTIVVTPLISLMKDQVDQLTELGVPSLFLNSSLDMISFRRNVEKL
                     RSNSVKLLYLSPEALLSQRILALLADLTIDCIAIDEAHCISEWGHDFRPEYRQLAEVR
                     TVFPSTSCVALTATATPRVREDICRSLNIDSQNEYVGSFDRPNLFLEVTPKIDAIEQV
                     LTLIDQNPHESGIIYCATRRETDKIYTTLKQKGYSVLPYHAGLSEKERAKNQELFSRD
                     DIRIIVATIAFGMGINKPNVRFVIHYNMPHSIDNYYQEIGRAGRDGLPAHCLLLFNYG
                     DIHKVRFFIEQKSESEQKVANILLDHVTGFAETDLCRRIPLLNYFGDANTSNSCGMCD
                     NCRMEDEKKVLDDLTIPAQMFLSCVKRTGEIFGAGHIINVLRGSKSQKIFKFEHEKLS
                     TYGIGLAYSARQWRYITRQLIQKGLLQNDYDHGSLKLTPKAWKVLRGEEVFFGKAGEA
                     GKEKATMQKAQQGEVDTYDTELFEMLRGKRKELADHANLPPYVIFHDRTLREMAFYYP
                     QTQESFIKLYGIGRSKLEKYADAFLDIIRMYCQTHQLQEKQQSSVVNQSFTAPQSNNE
                     KSHHKAIGKLFNQGHTIDQLASDFGVKRNTIIDHLYRYISQNGSLQKQYLIANSSLTK
                     VQWQSALSSFSRLGTNLLRPIFDDLNETISYDELKIVRLYVLNQEQACTDNTKTFVVL
                     AASRKYGGYCVAGKEWTDGKIGAWIRPVSQRTNGELVAENIRMNNNELPCLMDIVEVE
                     TQGPARHAYQKENFFAAGEKPWIWQLKLPDEALTKLLDFPESLWLNGFSSTNGLNDRV
                     PEEIVVKSNVPSIYLIQPDDFSLLVTDDLYGRKKVYARLTYREMSYLLSVTDMGIERE
                     YLMKPQDEYSIENKDIYLTISLGEPFNGFCYKLVAAVMMQKKDCQ"
     gene            complement(4270..5040)
                     /locus_tag="PLF77_04620"
     CDS             complement(4270..5040)
                     /locus_tag="PLF77_04620"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPC08170.1"
                     /translation="MRELKSFAVEIARNAGALLKKKFDKTHKIQYKGDINIVTEADKM
                     SEHLIIKSIRRNFPEHGILSEESPAIAGAGKIRWIVDPLDGTTNYAHGYPVFCVSIAL
                     ESDGNVVLGVIYDPMRDDLFSAVRGKGAYLNNKKLSVSSIRDISRSLLATGFPYDIRD
                     SEENNLDYFNAMAVNVQAIRRAGAAALDLAYLAAGRFDGFWELKLKPWDTAAGCLLVE
                     EAGGTISDVAGKMWNLYSSDLLASNGLIHEQMIKVLRM"
     gene            5190..5987
                     /locus_tag="PLF77_04625"
     CDS             5190..5987
                     /locus_tag="PLF77_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HPC08171.1"
                     /translation="MFIEQTPSDNSQKLKMKRESLGLSPDDVFKKIRIRTRYIEAIEN
                     SEFDQLPDPVYTKNFIKTYARFLNIDENPVLQDYDAYLNAHREEQTPPPEPPPETKSF
                     FASFAATKNYWGILLVLIIFISIWLILKQNQSVPEDINTPTAASSVPDTTPPAAPALT
                     DSQAPAIVPAPKTGVTDNPPATTDAKKSVLLITAGQETWLRIKPDNSPPVQLLLKPGE
                     KFESSEEFFNLDIGNAGGIKIQYKGRSIENLGKPGEVIHIRLPQVKQ"
     gene            6005..6190
                     /gene="tatA"
                     /locus_tag="PLF77_04630"
     CDS             6005..6190
                     /gene="tatA"
                     /locus_tag="PLF77_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPC08172.1"
                     /translation="MFGMGWQELVIVLIIVVIIFGVGKLPEIGSALGRGIKNFKKSIN
                     EQNEITDNSSKKEEDKK"
     gene            complement(6380..7135)
                     /gene="tpiA"
                     /locus_tag="PLF77_04635"
     CDS             complement(6380..7135)
                     /gene="tpiA"
                     /locus_tag="PLF77_04635"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPC08173.1"
                     /translation="MRKWIVAGNWKMHNTIAESIMLAKAIKDGSSDIQGGAVILAPPF
                     TALSAVGEAIKGSCLELAAQNMHYEDKGAFTGEVSPLMLKDIGCRYVIIGHSERRKYF
                     HEQNADVNLKVNKALSDGLIPIMCVGETDEEREKGITRDVVGRQVNHGLKGVEKIDHI
                     VIAYEPVWAIGTGKVATPAQAQDFHCFIRGILKESYGDMAGKISILYGGSVTRDNIGD
                     LIAMEDIDGALVGGASLKAEGFLGIIKTVAGGR"
     gene            complement(7176..8354)
                     /locus_tag="PLF77_04640"
     CDS             complement(7176..8354)
                     /locus_tag="PLF77_04640"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPC08174.1"
                     /translation="MKYIDQIDMKGKRVFLRVDFNVPMDKEGNVTSDKRVRASIPTIT
                     YALEKGAKLIVASHLGRPKGKRVAEMSLKPVVKVLSDLLKKEVLFLEDCVGPVVEQGV
                     ARLQDGQIILLENLRFQPGEDKNDAQFAKQLAALCDVYIDDAFAVSHRAAASNSAITE
                     FVPVCAAGFLLKNEVEYFKKAMVNPAKPLAAIIGGAKVSDKIGLLENLIEKVDKIIIG
                     GGMAFTFLRAAGYETGKSLCEEDMLDTARKIVDKAKQKNVQFLLPVDAVIAQAAEAQA
                     EARICGVQDIPKEWIGLDIGPATIALFSEALKGVKTVIWNGPMGMFELAPFSKGTFQL
                     AQAVADSGALSILGGGDTDTAIKKAGLTSKMSYISTGGGAFLEWLEGKTLPGIAALEK
                     "
     gene            complement(8369..9367)
                     /gene="gap"
                     /locus_tag="PLF77_04645"
     CDS             complement(8369..9367)
                     /gene="gap"
                     /locus_tag="PLF77_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPC08175.1"
                     /translation="MSVKIAINGFGRIGRLVFRAGYQNKDVDFVAVNDLADAKTLAHL
                     LKYDSTHGILDADISVANNAIIVDGKELKTFSVREPETLPWKDLGVDVVLESTGKFTD
                     KTGAGKHMEAGAKKVVVSAPAKNPDVTFVLGVNQEVYDKNKHHIISMGSCTTNCLAPI
                     VKILHQEFGIEYGLMTTIHSFTNDQVVVDEPHRDLRRARAAALSMIPTTTGAARAIAE
                     VIPEMKGKLDGLAIRVPTPNVSLVDFVATLSKSTTRNEINDKFREYAQSAMKGILACS
                     EEELVSKDFNGNKHSSIVDMPNTTVIGGNMVKILSWYDNEWGFSNRMLELLSFIMK"
     gene            complement(9522..11051)
                     /locus_tag="PLF77_04650"
     CDS             complement(9522..11051)
                     /locus_tag="PLF77_04650"
                     /inference="COORDINATES: protein motif:HMM:NF010184.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HPC08176.1"
                     /translation="MIQQPGFDNEKYIQEQTTEILKRVNRFNNKLYLEFGGKLLYDYH
                     AARVLPGYDPNVKMRLIRELKDKADIVLCIYAGDIERKKIRADFGITYDADALKLIDN
                     LRTWGINVLGVVITRFEEQPAAIIFKNKLERRGISVYTHRFTKGYPTDVETIVSDEGY
                     GANTYIETDKPLVIVTGPGPGSGKMATCLSQLYHDYRRGIKSGYAKFETFPIWNIPLK
                     HPVNVAYEAATADIRDFNLIDPFHLDAYSEKSVNYNRDVEVFPVLKRILEKITGEPSF
                     YKSPTDMGVNRAGFAIIDDAITQKAAKMEIIRRYFRYRCEYAMGFADRETVQRVELFM
                     KDFNLEPEERPVVQPAREAAVTAPVETRDYERGNEGIFCGAALELKNGKIITGSNSSL
                     MHAATSMVLHAIKHLAEIPDKIKLLPPQITDSVKNLKTEILDEKSVSLDLEEALIAIS
                     ISATNNPAAQLALEKLRELRDCEAHMTHIPTPGDEAGLRRLGINLTTDPNFSTNDLFI
                     T"
     gene            complement(11417..11953)
                     /locus_tag="PLF77_04655"
     CDS             complement(11417..11953)
                     /locus_tag="PLF77_04655"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPC08177.1"
                     /translation="MKKKDKRFVGSLAFVFALLFVISSAGAALADAVSAKIGFVDIAK
                     IMRDSRAAQNARAVYQKDFESKKATLKAKGDRVSSLEKDLSNTKQDSPALKEKREKLA
                     QEFKEFKRLESDLNEQLKKKDAELTRKILMDVQEILKQFSKNEKYSMIFEKKAALVVD
                     DGFDVTDKIIKIYDAQKK"
     gene            complement(11990..13645)
                     /locus_tag="PLF77_04660"
     CDS             complement(11990..13645)
                     /locus_tag="PLF77_04660"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HPC08178.1"
                     /translation="MANRSTQKRAVKIPRGKALLCDPGLNKGTMFSDTERDVLGLRGL
                     LPPRVFSPSDRTVHIINNVRRKTTDLEKYLYLSGLQNRNERLFYRVLIEHIQELMPVI
                     YTPTVGQACQEYSSIFSEPRGLFISKNDRGSIKKILKRWPQKKVRVIVVTDGERILGL
                     GDLGANGMGIPVGKLALYAACAGIDPEACLPVTLDVGTNNPELLAAPDYIGLPQPRVR
                     GREYDQLVDEFMAAAANVFPGVMIQMEDFGNANAFRLLEKYRSKYCMFNDDIQGTASV
                     ALAGLYAALRITGGSISRQRLLFLGAGEAAIGIGDLVSSAMMEEGLSLPEAHSNCWFV
                     DSKGLVVKGRTDLVEHKKPYAHDYPFHRDLLSAVEALKPTAVIGVSGQPGTFTPEIIK
                     AMGRINRRPIIFALSNPTSKAECTAEAAYRHTEGRAIFASGSPFGEVSMEGKTFVPGQ
                     ANNAYIFPGVGLGVLASEAVRVTDEMFTAAAKSLNKQVSADDLKIGRIYPSLTTIREV
                     SAKIALAVAEVVFKRSLTRMQAPADLRGHIRATMYEPDFGTYI"
     gene            13890..14216
                     /locus_tag="PLF77_04665"
     CDS             13890..14216
                     /locus_tag="PLF77_04665"
                     /inference="COORDINATES: protein motif:HMM:NF014279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HPC08179.1"
                     /translation="MAEKKGLSKPVKLKGDLAELLGAKALPRTEITKKLWDYIKANKL
                     QTTKVNGKPENAGKNIVIDAKLIKIINNTKVKTSSGKVVDFTKLKEGQTIDMMQIASV
                     VSANVE"
     gene            14438..15004
                     /locus_tag="PLF77_04670"
     CDS             14438..15004
                     /locus_tag="PLF77_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPC08180.1"
                     /translation="MDKKAILWPVIISVVIHMTLLAVAGMIDLNDKIKPVNVLSVSIQ
                     EPAPAQPSPEKENPAQGNPSSSVTKQQNDPTPDDAWREDTINLGSLDTKYIHYLTRVK
                     NKILRIWKYPQTSFERNEEGNVVVRMSIDADGRLAAVTLVSSSGSADLDNGALGVVQE
                     AAPYEPLPGSYNLSRLHIVASFNYKIMD"
     gene            complement(15045..16664)
                     /locus_tag="PLF77_04675"
     CDS             complement(15045..16664)
                     /locus_tag="PLF77_04675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC08181.1"
                     /translation="MKVKFLGAAREVTGSCFIIETDQARFAVDCGMHQGGADVETRNW
                     DTEAYDPSHIDFFLITHAHIDHSGLLPRMVQNGFKGPVYATEPTGELIKILLLDSAHI
                     QETDAKSKTRRMQRNGRTEKSEPLYSTPDAEAVSPLIKKITYNQNFTPAAGIKINFQD
                     AGHILGAAILELFIEENGTSTKLVFSGDIGRRHQLLMEDPVTISDADYLFMESTYGDR
                     DHKGEEDSLNEMAEAIQYSYSRGEKIIIPAFAVERTQEMLYSLYLLDIKGKLPKDMPV
                     FLDSPLAIKATEIFRRHHTYLDADTQNILKNGEDPLDLPQLKFSSSTEQSMRINEMEG
                     SAIVISASGMCNAGRIRHHLRHNLWRPGASIVFVGFQAQGTPGRKIVEGAKKIRIFNE
                     DIAVKAKIWTIGGFSAHAGQRQLLDWLGHFQNKKMPVYLVHGEAGAQDTLASLIRERL
                     GFEVAIPEYLEEINIKAGTRPQEIKQPRPSRPPLELAPLLADLKAKIDYINARVDKIQ
                     SLPESEQQEVIQLLRQVNQNMDDIKPDLQTG"
     gene            17111..18040
                     /locus_tag="PLF77_04680"
     CDS             17111..18040
                     /locus_tag="PLF77_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC08182.1"
                     /translation="MIETRPIEPVIVARNLEKVFGAFKAVDDISFHVVPGVCFGILGP
                     NGAGKTSTIRMLYGFSPLTAGDIFVFGLNIKTHDRKIKARIGVCQQENTLDPDLTVMQ
                     NFEVFARYFDIGKKTALARATTLLKFFALEQRQNARATELSGGMVRRLVIARALINQP
                     DLIILDEPTTGLDPQSRHQVWERLEQLKNQGMTILLTTHYMDEAARLCDNLVIMDHGK
                     ILAQGSPRSLIREYTGGHIIEVAEPDEALRAFSQEKHDRVEDLGHRLIIYAANGHELY
                     QELCDRFSQKSCILRSATLEDVFLKLTGRELRE"
     gene            18037..18816
                     /locus_tag="PLF77_04685"
     CDS             18037..18816
                     /locus_tag="PLF77_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC08183.1"
                     /translation="MTFRISTRFIRVWQRNLTVYRESWKINFMPPLLEPLFYLLAFGI
                     GFSGLIGSVTYAGTEISYVMFIAPALLAINIMNNSFFENTFGSFVRMYYQKTFDAMMA
                     TPLTVDEIVTGEIVWGATKSVIGTIIMLIVIGFFGLISYPAGLLIVLVAFIGGFAFGS
                     VAMIFTGLVGKIELFNLPVFLFITPMFLFSGTFFPISGLPLWAQYFALTLPLTHLVNL
                     VRALGLGAFNAEALAGFCYLIIFCLITFPLALSVMRKKLIK"
     gene            19089..19424
                     /locus_tag="PLF77_04690"
     CDS             19089..19424
                     /locus_tag="PLF77_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPC08184.1"
                     /translation="MNKNLYVGNLSYKITGDDLKSNFSEAGEVVSATIIKDKFSGQSK
                     GFGFVEMKTEEGAAEAIKKFHGGTLDGKEITVNEARPKKEFSGGHRGGGNRGGGGGFN
                     RGGRRDGRY"
     gene            complement(19581..20192)
                     /locus_tag="PLF77_04695"
     CDS             complement(19581..20192)
                     /locus_tag="PLF77_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC08185.1"
                     /translation="MKKKNTFAELKNNEKRARQGVIVEAAQRVFAVKPFNKVSIRDIA
                     REAGISHASIYRYFPDQQSLFVEAFLHGAEEITAILDRMIKNSRKNPIEKVADAYLSY
                     LIDNDQYFRMMTRFMLDGSLSADMVEKLNASERRIFDQFDVLFRKMNLPEPVRPLSHA
                     FFAALNGVLITFRNYPGRSAKAVHQHMHNIVRVIVSKFCVPSA"
     gene            20321..21910
                     /locus_tag="PLF77_04700"
     CDS             20321..21910
                     /locus_tag="PLF77_04700"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPC08186.1"
                     /translation="MSLSDRNNPYTFDDFLKWRQGVDYYRDDPFIQKVVHHFTGDIRQ
                     QVDAEARACSQKASFRWRDFADSIARPEKRPFMLHYDGHHNRIDRIVRPHETEIMEKE
                     IFSEAFFADTTSPWVKLIKMYVIYQNGEACIGCPVTCTEGLVMLLEKYADSPETKEIL
                     KHCKDGINGDFAIGAQYLSEIQGGSDVPANVLEAVNENGTWKLYGTKFFCSATHADYA
                     VVTAKPVGSEKVALFVVPSWLPGNKEKEMRNGYTVDRIKWKMGTSELTTAELTFNGAV
                     AYPVGPLDKGVANVVGIVLTYSRLTVGLSAAAYMTRAVREAKKYTEFRNAFGLTIGQF
                     PLVAVQVNRIDRMAKRTTAGAFKLYRDFLTLEGGLKGGLATDEPEAVRKNRFNIRELI
                     MLQKMAAAWDCTDVIRESMSIFGGHGVMEDFSSLPRLYRDSAINELWEGPRNVLLTQI
                     HRDLQKVSGWYKPAEFVRHILSGLNESTIKELADEITELVAYPNLFQPDEKTMDICRR
                     WDAFCHRFFHAYQDLALKEVE"
     gene            complement(22036..23004)
                     /locus_tag="PLF77_04705"
     CDS             complement(22036..23004)
                     /locus_tag="PLF77_04705"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08187.1"
                     /translation="MIAVQGYPMTLSVLLFIAGIVLLWAGTEFVLQRVPILAGWLRVS
                     PLVVTVLLLAVMTSLPEFCVSLFASMRGQPAAAVGNVVGSNFVTLTFVAGICALWRPI
                     IVGPTLRERESSWMILSAALLLVIAMDGVISRIDGLILLMAYGPYLKGTIDEARQQRK
                     EAPVVSMAKPWLHGIIFVAGIAMIVLGSSWIVENGTMLARRLGMNDLLIGVTFYAFGT
                     SLPELAIALGAVLRRQADVTLGEIYASNIFTGLAVIGCLCLIKPLPVEPIIVQRDLPM
                     LIVAGVLLQMFVTTGRKFVRLEALVMIAIYVLFLAAQFMGFSISLS"
     gene            complement(23075..24409)
                     /locus_tag="PLF77_04710"
     CDS             complement(23075..24409)
                     /locus_tag="PLF77_04710"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPC08188.1"
                     /translation="MRFEISRGGSGLTYEIRNIVLIANKLKEYGVDIIWENIGDPVQK
                     GEKIPDWMKEVLFEIIKDDESFAYSPTKGVNATREFLTERLNARGNVRITPEDIIFFN
                     GLGDAIARAYSSIQVDARMIMPEPTYSTHFMAEVLHASFPPNTYRLNPYNGWHPDMRE
                     LEQKVRSHKAIVGILVINPDNPTGFVYPEETLRNIVRIAKENDLFVVFDETYINIVYN
                     GKNTVPLSDIVGSVPAISMKGISKELPWPGSRCGWMEVYNAGKDPIFDRFINTILHQK
                     MSEVCSTTLPQLAIPKIMTHPEYDKYLQERRRHYEKLSDIAYNILKDVPCMMVNKTDG
                     AFYMTIVFNESGLNSNQKLHIKNGEIREFVENLVKEPIEHDKRFVYYLLAATGICVVP
                     LTSFFTPLLGFRMTLLDKDVDEFEHTVKTIAEKFTEYIDSSSKGMQTAIKLY"
     gene            24534..25697
                     /gene="rpsA"
                     /locus_tag="PLF77_04715"
     CDS             24534..25697
                     /gene="rpsA"
                     /locus_tag="PLF77_04715"
                     /inference="COORDINATES: protein motif:HMM:NF005208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HPC08189.1"
                     /translation="MTEEKSFSEMLDETSLKPRRFSAGEKIDTIIVKITEEWMFLDLG
                     AKSEGYLDKKELLDENGNLTVKEGDPITAYFVSSRHGEKLFTTKLLTSKSVDDFMFKA
                     YKNQIPVEATVEKEIKGGFSVRINQNVSGFCPYSQMDVRRIDDVTAYVGKKFEFIVAE
                     YSEKGRNIILSRRPLLEKIEQEKKAALKNSLQQGMTVSGVVTSVQKFGAFVDLGGIQG
                     LMPVSEMGWGRVEDPKVLYSPGDKIEAVIINLDWEANRITLSAKASLPNPWDGFVRKY
                     TEGSLLKGKVTNLTNFGAFVSLEDGVEGLLHISKLARGKKIKHAGDVMKTGEELEVKI
                     EKIDRENRKISLDLTGSDKDTSAASGNEDDFRGYIGKSPKTMGTLGDLIKKKK"
     gene            complement(25832..26992)
                     /locus_tag="PLF77_04720"
     CDS             complement(25832..26992)
                     /locus_tag="PLF77_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08190.1"
                     /translation="MRKNIITKILLSRKLYQLSLENLNSDNELSLSIGVILLQDSVEI
                     FLLAVAEHIDASIGNNSNFNQYFDLINKKLSPKELPFKLRLTALNKLRVNAKHYGLSP
                     SKLETHGLIETVKEFFEEVSIQVFEKEFVTISLVDLINKGEAKDYLMAAENDFSKGDY
                     VNVLINCRKAIYVEFETDYDISLYGDEREKIMGTMAIMLTGMKAPFYVCNKKYIDEYV
                     KEPTDYIVYDHDRLDMDLLRAGISRVDYWNVWRLTPSLYRKNKESDWIVKKDFQKLDE
                     EGIKDRAEYVLDSSINLILTKHLDIKRSKSPDYKKYYVSLKNESVPIYEKADNKSKVV
                     GTVPANCKKVFVDSKVSALNGKGIFWHVVHVSVEDNFYLWGYIDNDDIINSN"
     gene            complement(27179..>28035)
                     /locus_tag="PLF77_04725"
     CDS             complement(27179..>28035)
                     /locus_tag="PLF77_04725"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPC08191.1"
                     /translation="ISSFFDHEDNYIKGYQDTNTPVFYSPENTARALGSLYLYKQIKE
                     RAPRKEAALPKIKKEATEIILKALDNKQKALDEYEAKQLLACYGVPVTKEALAAKVED
                     ALKTAKKIGYPVALKACSWKIMHKSGKGLIALNVENENQLKKEFENIQKNAGKTAPVL
                     VQEMLSGSREFLAGMTRFAGFGPGVVFGLGGVFTEVFKDTTMRLAPVTSADALEMFED
                     INARKLLDKFRGLPKVKLSNLSRIIQALGNIALLHPQIAEIDLNPIIINGAEPVVADA
                     LIVLEDKS"
BASE COUNT         7106 a         6926 c         6635 g         7368 t
ORIGIN      
        1 aggccgtccg ggatgaaggc gtacagtccg gtgtttgtct ggtctacacc ccgcacacca
       61 cggccgcaat caccatcaat gaaaacgccg atccggatgt gccccgtgac atcctggccg
      121 cgctggaccg agcggttccg ctgtcggcca attaccgtca cgcggaaggg aactccgcgg
      181 cacacgtcaa atcgtctctc gtcggagcct ccgaactggt gatcatcgaa aacggacatc
      241 tggtgctggg aacctggcag tccgtttttt tctgcgaatt tgacggtcct cgcacaagaa
      301 agttcatggt aaaagttatt ccagattgat aaagctttcc ttgaaatgat cagagatgaa
      361 ttattcttac attttcccat ttagattgaa aatattcaac tacaagtcgt cgatggcatt
      421 gtgtgggttt ttcttcgctg cataaaaggc aggcttcatg caaaagttca ggcttgattt
      481 cagtttcgac ctttcttgac gctagcaacg caagaaaatc tttttcataa atctcccaac
      541 tgcctttatt tttcttgaaa ttgtcaagga tttgctgcgt gggagccaga tcgggacgat
      601 ggacataatc cattttgcca atcgtccaga gaaagtagca aaggtcatcg cgcttggcaa
      661 agccagccag ttgagaaaca ttgttgaggc ggatatcaat cacccgcttg accccggccc
      721 ggattaaacg cgagaaaaac tcctcggcag ttttcttcgt aaaaccgata gtaaatatct
      781 ttattccatt cataatgttt cctgtctctc ctccagtgta taagcgactt tatttccctg
      841 tagatcatag gcgcgttgaa tttgctcctc ttcagtcgaa aaaagatctg cagggtctat
      901 tttcaacatc ttcatcaaac gcatttcggt gtccctatta tcttccagcg atccgtcttc
      961 caggatatgc tgaatatgga tgttttcttc ccgcaagttg cggcagacaa gtagcatccg
     1021 gtggcatgtg agcggatctt tttccgcgca catgagggcg atccgataag tctccagacc
     1081 tttgcgcaaa cgagccagcc cctgctgaaa aatctccttt tcggccaggc gtttatattg
     1141 gaccttaccg ttttcatagc aggactgatc tgagctccta gggcccagct cagcgcccag
     1201 gtaaatatac gcgatgcctt gtttcagaac gtcctctttc aaagattcgc ggttgaattg
     1261 cggcgaataa cggctgtaag gactggaccg gacatcgcac agagcgttga tggcgtgcag
     1321 cttgagcatt tccagaaact cttccacagt atgaaccgaa tgcccgacgg tgaacaattc
     1381 tttcattggc aatccttttt ttgcatcatc acagccgcaa ccagtttgta gcaaaagccg
     1441 ttgaacggtt cgcccaaact gatggtgagg taaatgtctt tattttctat cgaatactcg
     1501 tcctgcggtt tcatcagata ctcccgttca atccccatat ccgttaccga aagaagatag
     1561 gacatttccc tgtacgtaag tctagcatag actttttttc tcccgtacaa atcatccgtc
     1621 accagaagcg aaaaatcatc cggctggatc agataaatag aagggacatt actttttacc
     1681 acaatttctt cggggacgcg atcattcaaa ccgtttgtgc tgctgaatcc gttcagccag
     1741 agactctccg gaaaatccag cagtttggtt aaagcctcgt caggcagttt caattgccaa
     1801 atccagggtt tttctcccgc ggcaaagaaa ttttccttct gataggcatg gcgcgccggc
     1861 ccctgcgttt caacttcaac gatatccatc aagcacggaa gttcgttgtt gttcatccga
     1921 atgttttccg cgacaagttc gccattggtc cgctggctga cgggacgaat ccacgccccg
     1981 atttttccat ccgtccattc cttgcctgca acacagtagc cgccatattt ccgcgatgca
     2041 gcaaggacaa cgaaggtttt ggtgttatca gtacaggcct gttcctgatt taaaacatac
     2101 aaacgaacaa tttttaattc gtcgtaagag atcgtttcat tcagatcgtc aaaaatggga
     2161 cgtaataggt tcgtgcccag acgtgagaaa gaagacagag cagattgcca ttgcactttt
     2221 gtaagagagg agtttgcaat aaggtattgt ttctggagtg aaccattttg gcttatatag
     2281 cggtatagat gatcaattat tgtatttctt ttgacaccaa aatctgaggc caattgatca
     2341 atggtgtgac cttgattgaa tagcttacca attgctttat gatgcgactt ctcattattt
     2401 gattgaggtg ccgtgaaaga ttgattgacc acggaagatt gttgcttttc ttgaagttga
     2461 tgggtttgac aatacattcg aattatatcc agaaatgcat cggcgtattt ttccagttta
     2521 cttctgccaa tcccataaag ttttataaaa ctttcttgag tttgaggata atagaaagcc
     2581 atttctcgta acgtacggtc gtgaaaaatc acgtatggtg gtagatttgc atgatcggcc
     2641 agttcctttc ttttcccccg gagcatttca aatagttccg tatcgtacgt gtcaacttca
     2701 ccttgttgcg ctttttgcat cgtagctttc tcttttcctg cctctcccgc tttgccgaaa
     2761 aagacttcct ctccccttaa tactttccat gcttttggag ttagtttaag cgatccatga
     2821 tcatagtcgt tttgcaacaa gcctttctga atcaattgac gggtaatata acgccattgt
     2881 cttgcggaat aagctaagcc gattccatac gtcgataatt tttcatgttc aaatttaaaa
     2941 attttttggg attttgagcc tctgagaaca ttgataatat gacctgcgcc aaaaatttct
     3001 ccagtccgct tcacacagga gagaaacatt tgagccggaa tggtcaaatc atcaagcacc
     3061 tttttttcat cttccattcg acaattgtcg cacataccac agctgttcga cgtgtttgca
     3121 tctccaaaat aatttagaag agggattcga cgacataagt ctgtttcggc aaagccagtg
     3181 acatgatcca gaagtatatt ggctaccttc tgttcacttt ccgatttttg ttcgatgaaa
     3241 aaacgtactt tatgaatgtc tccataatta aacagaagaa gacaatgggc cgggaggcca
     3301 tcgcggcccg cccgtccgat ctcctgatag taattgtcga tgctgtgggg catattgtaa
     3361 tggataacaa acctgacatt gggcttgttg attcccattc cgaaggcaat ggtcgctaca
     3421 atgattcgga tatcatctct gctaaataat tcttggtttt tggcacgctc tttttctgaa
     3481 agtcccgcat gataagggag gacagagtaa cccttttgtt tcagggtagt gtaaatttta
     3541 tccgtttccc gacgagtggc gcaataaatg attcctgatt catggggatt ttgatcaata
     3601 agggtcaata cttgctcaat ggcatcgatt tttggggtca cttccaaaaa tagattcgga
     3661 cggtcgaaag aacctacata ttcattttga ctgtcaatgt ttaaagaacg gcaaatgtcc
     3721 tcccgaacac gaggtgtagc cgttgccgta agagccacgc agctcgtcga tggaaacacg
     3781 gtgcgtactt ctgccagttg ccgatattcc ggacgaaaat cgtgtcccca ctccgagatg
     3841 caatgagctt cgtcaatggc aatgcaatct atcgttaaat cggctaacag tgcaaggatt
     3901 ctctgagaca agagggcttc cggtgataag tagagaagct taacgctgtt gctgcgaagc
     3961 ttttccacat tgcgtctgaa cgagatcata tccagtgagc tattcaaaaa tagacttggc
     4021 actccaagtt ctgttaattg atcaacctga tctttcatga gggaaatcaa cggtgtaaca
     4081 acaatcgtta gaccatcaaa gattattgac gggagctgat aacacaggga cttcccccct
     4141 cccgtgggca ttacaataag cgcatctctt ttattcagga tatgagcaat aattttttct
     4201 tgaagaggta agaaggtatc gaatccaaat gtatttttta aaatagattg tgcacgattc
     4261 attactattc tacatcctta aaaccttaat catctgctcg tgaatcaaac cgttgctggc
     4321 cagaagatcg gatgaatata aattccacat ttttccggca acatcggaaa tcgtgcctcc
     4381 tgcttcctca acgagaagac agcctgcggc cgtatcccac ggtttgagct tcagttccca
     4441 gaaaccgtcg aaccttcccg ccgcgagata ggcgagatca agggccgcgg cgcccgcgcg
     4501 ccggatcgcc tgcacgttca cagccatcgc gttgaagtaa tccagattat tttcttcgct
     4561 gtcccggatg tcatagggga accccgtggc caggagactg cgggaaatat cccggataga
     4621 ggaaaccgac agtttcttgt tgttcagata agcccccttg ccgcgcacgg ctgaaaataa
     4681 atcgtctctc atggggtcat aaataacgcc cagaacgacg ttgccgtcgc tttccagcgc
     4741 aatggaaacg caaaagacag ggtatccgtg cgcgtaattt gtggttccgt ccaggggatc
     4801 aacaatccag cgtatttttc cggcgccggc aatggccggc gattcctccg acaagattcc
     4861 atgttccgga aaattccgtc tgatggattt tataatcaaa tgctcggaca tcttgtccgc
     4921 ttccgtcaca atattgatat cgcccttgta ttgaatcttg tgggttttgt cgaatttctt
     4981 tttcagtaac gctccggcgt ttcgcgcaat ctcgacggca aacgatttta attcccgcat
     5041 aaaaaatact cctgtcccgt tttcttcggc atactaacaa taaaatcaga aaatattaaa
     5101 gtaataattg tttttatttt aaatagttgt attgcaacca aattaatgct aatacacaaa
     5161 caaaaaaatg gcttcatctg tgaatctcca tgtttatcga acagacaccg agcgacaact
     5221 cgcaaaaact gaaaatgaag agagagtctc tggggctttc tcctgatgat gtatttaaaa
     5281 aaatacgcat cagaacccgt tatattgaag ccatagaaaa tagcgagttt gaccagctgc
     5341 cggatccggt ttatacaaaa aatttcatta aaacctatgc gcggtttctc aacatcgacg
     5401 aaaatcccgt tttgcaggat tacgacgcct acctgaatgc acaccgcgaa gagcagacgc
     5461 ccccgccgga gcccccgccg gagacaaaat ctttttttgc ctcatttgcc gccacgaaaa
     5521 attactgggg aatcttgctc gtcctgatca tttttatcag catctggttg attctcaaac
     5581 agaatcagtc tgtccctgaa gatattaaca cgccgacggc agcctcaagc gttcccgata
     5641 caacgcctcc tgccgccccg gcattaaccg acagccaggc gcccgcgatc gtccccgcgc
     5701 caaaaaccgg cgtgacggac aatccgccgg cgacaaccga tgcaaaaaaa agtgttttat
     5761 tgattacggc cggtcaggaa acgtggcttc gcataaaacc ggacaacagt ccgccggttc
     5821 agcttctgtt aaaaccggga gaaaaatttg aaagcagtga ggaatttttt aatctggata
     5881 ttggcaatgc cggaggaatt aagatacaat acaaaggcag gtctatagag aacctgggga
     5941 agccgggaga ggtgatccat ataagattgc cgcaagtcaa acaataaaaa tcaagaggta
     6001 aggtatgttc ggaatgggat ggcaggaact cgttattgtt ttaatcattg ttgtgattat
     6061 ttttggtgtc gggaaattgc cggaaatcgg ttcggctctg ggcagaggca tcaagaactt
     6121 caaaaaatcc atcaatgagc aaaacgaaat caccgacaat tcatcaaaaa aggaagaaga
     6181 taaaaaataa ctgctgacac gataagcatc ataacccgtt tttcatccgg ttatgatgct
     6241 cgcgcagata tccttcccgc cttttgcatt cttttttcca gtgcgaaaac catggtgttt
     6301 ttatcgcgca agatgcggac gaacggcgtc aaacaatccg ttttcggcac gcggcacgct
     6361 tgcgacgttt atccccgaat tatcttccgc cggcaacggt cttaatgatg cccagaaaac
     6421 cctcggcctt taatgacgct ccgccgacca gcgcaccgtc gatatcctcc atggcgatca
     6481 aatcgccgat attgtcccgg gtaacgctgc cgccgtagag gatactgatt tttccggcca
     6541 tgtcgccata ggactccttt aaaataccgc ggataaagca atggaaatcc tgggcctgcg
     6601 cgggcgtggc cacttttccg gttccgatgg cccagaccgg ttcgtacgcg atcacaatat
     6661 ggtcgatttt ttcaacacct ttcaggccgt gattaacctg cctgccaacc acatcccgtg
     6721 taatgccctt ttctctttct tcgtcggtct ctcccacgca catgatcggg atgagcccgt
     6781 cggacaaggc cttgttcact tttaaattga catcggcatt ctgctcatga aaatatttcc
     6841 ggcgttcgga atgaccgatg atgacatacc ggcagccgat atctttgagc atcaacgggg
     6901 acacttcccc ggtaaatgca cccttgtctt cataatgcat gttttgcgcc gccagttcca
     6961 gacaggagcc tttgatcgcc tcgcccacag ctgaaagcgc ggtaaaaggc ggagccagga
     7021 tgacagctcc gccctgaatg tctgatgatc cgtctttaat ggctttcgcc agcatgatgg
     7081 attcggcaat cgtgttatgc attttccagt tgccggcaac aatccatttt ctcatcgtta
     7141 tgtcctcacg atcttgttgg gggtcctgat aaccgttatt tttccaatgc agcaatgccg
     7201 ggcagcgttt tgccttccag ccattccaaa aacgcgccgc cgccggtgga gatataagac
     7261 attttcgacg tcagaccggc tttcttaatg gcggtatccg tgtcaccgcc gccgaggatg
     7321 gatagcgctc cggaatccgc caccgcctga gccagctgaa acgttccttt gctgaaaggg
     7381 gcaagttcaa acatccccat gggaccattc cagatgaccg tctttacgcc tttcaaggcc
     7441 tcgctgaaca atgcgatggt tgccggaccg atatccaggc cgatccattc tttgggaata
     7501 tcctgcacac cgcagatcct ggcttcggct tgagcctcgg cggcctgtgc aatgacggca
     7561 tccaccggca gcagaaactg tacgttcttc tgtttcgcct tgtccacgat ctttcgcgcg
     7621 gtatccagca tatcttcctc gcagagcgac ttgccggttt catatcccgc cgctctcaaa
     7681 aaggtaaagg ccattccgcc gccgatgatg attttgtcca ctttttcgat caggttttcc
     7741 aacagaccga ttttgtcgga aacctttgcc ccgccgatga tcgccgccag cggtttggcc
     7801 ggattcacca tggctttttt gaaatattca acttcgttct tcaacagaaa accggcggca
     7861 catacgggaa caaattcggt aattgccgaa ttggaagccg ccgcccgatg cgatacggcg
     7921 aaggcgtcgt cgatatagac gtcgcaaagc gccgccagtt gtttggcgaa ttgagcatca
     7981 tttttatctt cgcccggctg aaaccggaga ttctccaaaa ggataatctg gccatcctga
     8041 agcctggcca caccctgctc cacaaccggt cccacgcaat cctccaggaa gaggacctct
     8101 ttcttcagaa gatccgaaag aaccttgacg acaggcttga gtgacatctc ggcgactctt
     8161 ttgcctttcg gcctgccgag atgggaggcg acgatcaatt tcgcaccctt ttccaacgcg
     8221 taggtgatgg tggggatact ggctcgcacg cgtttgtcgc tggtcacgtt tccttcttta
     8281 tccatgggga cattgaaatc cacccgtaaa aaaactcttt tgcctttcat gtcgatctga
     8341 tcaatgtact tcatcttgaa caccttccct atttcattat aaacgagagc agttccagca
     8401 ttctgttgga aaagccccat tcattatcat accaggagag aatcttgacc atgttgccgc
     8461 cgataaccgt cgtgttgggc atatccacaa tggatgaatg cttgttgccg ttgaaatctt
     8521 tggaaaccag ctcttcttcc gaacacgcaa ggataccctt catggcgctt tgggcatatt
     8581 cacggaattt atcattgatt tcatttcttg tggtactttt ggaaagtgtt gccacaaaat
     8641 ccacaagcga aacattcggc gtcggcacgc gaatggccag accgtcgagt tttcccttca
     8701 tctcaggaat gacttccgct atggcccggg ccgcaccggt ggtggtgggt atcatggaaa
     8761 gagccgccgc gcgtgctctt cgtaaatctc tgtgaggttc atccaccaca acctgatcat
     8821 ttgtgaaaga atgaatggtc gtcatcagac cgtattcgat gccgaattcc tgatgcagta
     8881 ttttgacgat cggcgcaagg cagttcgtgg tacaggaccc catcgaaatg atatgatgct
     8941 tgtttttatc gtatacttcc tgattgaccc ccagaacaaa cgtcacgtca ggatttttag
     9001 caggcgccga cacgacaact ttttttgccc ctgcctccat gtgttttccg gcgccggttt
     9061 tatcggtgaa tttccccgtg ctttccagaa caacatcaac gccgagatcc ttccagggca
     9121 gcgtctccgg ttctcggacg gaaaaagttt tcagttcttt tccgtctacg ataatcgcgt
     9181 tgtttgcgac gctaatgtcg gcatcgagta tcccgtgtgt ggaatcatat ttcaaaaggt
     9241 gcgccagcgt tttggcgtcg gcaagatcgt ttaccgcaac aaagtcaaca tctttgtttt
     9301 gatatccggc cctgaaaaca agccgcccga ttcttccaaa accgttaatg gcaatcttca
     9361 ctgacatagt atgacctccc ttcatgtctc aaaaattatt aattaaaagg gggggctttg
     9421 tcaacctgtt ttgccgaagc cgcaagcgaa aagagcatgg cattcgagat gattctctaa
     9481 agaaaaagga tagttacgat tcgcagccgg tcggtattat tttatgtaat gaataaatcg
     9541 ttagtggaaa aattcggatc cgttgtcagg ttgatgccga ggcggcgcaa ccctgcttca
     9601 tcgccgggtg tgggaatgtg cgtcatgtgc gcttcgcagt ccctcagctc cctgagtttt
     9661 tccagcgcca gttgcgcggc gggattattt gtcgcgctga tgctgatagc gatcagagct
     9721 tcctccagat caaggctcac cgatttttca tcgagaatct ccgttttcag atttttgacc
     9781 gaatcggtga tctggggcgg cagcaattta atcttatccg gaatttccgc cagatgcttg
     9841 atggcatgca acaccatgct ggtggcagcg tgcatcagtg atgagttgct gccggtgatg
     9901 atcttgccgt tcttcaattc cagcgctgcg ccgcagaaga tgccctcgtt tccgcgttcg
     9961 taatcccgcg tttccacggg agcggttacg gcagcctcgc gcgccggttg aacaaccgga
    10021 cgctcctccg gctccagatt aaaatccttc atgaaaagtt ccacgcgctg cacggtttcg
    10081 cgatcggcga atcccatggc gtattcacag cggtaacgga aataacgacg gataatttcc
    10141 atcttggccg ccttttgcgt gatcgcgtca tcgataatgg caaacccggc gcggttgacc
    10201 cccatatccg tcggcgattt gtaaaaagac ggttcgcccg taattttttc cagaattctt
    10261 ttcaggacgg gaaaaacttc cacgtcgcgg ttataattga cggatttttc gctgtacgcg
    10321 tcaagatgaa acggatcgat cagattgaaa tcccggatat cggcggtggc cgcctcatag
    10381 gccacattca caggatgctt taatggaatg ttccagatgg gaaatgtttc aaatttggcg
    10441 taaccggatt taatgccgcg ccggtaatcg tgataaagct gcgaaagaca ggttgccatt
    10501 tttccgctgc ccggccccgg accggtcaca atcaccagcg gtttgtctgt ttcgatatag
    10561 gtatttgcgc cgtatccttc atcgctgaca atggtctcca catccgtcgg atagcctttg
    10621 gtaaagcggt gcgtataaac ggaaataccg cgtctttcca gtttgttttt aaagatgatc
    10681 gcggcgggtt gttcttcaaa acgggtaatg accacgccca gcacattgat tccccaggtt
    10741 cgcaggttgt caataagctt taaagcgtct gcgtcatagg taatgccgaa atcggcgcgg
    10801 attttttttc tttcgatatc cccggcataa atgcaaagca caatgtcggc cttgtctttt
    10861 aattcccgga tgaggcgcat cttgacgttg ggatcataac ccggcaggac gcgggcggca
    10921 tgatagtcgt ataaaagttt gccgccgaat tccagataca gtttgttgtt gaagcggttg
    10981 acgcgtttga ggatttcggt ggtctgttcc tggatgtatt tttcgttatc gaaaccgggc
    11041 tgttgaatca tctgagtttt tctcctgaca taaaattgat ctatcccgaa aacgtttcat
    11101 ttttgaacta taagtagaac aattttgtaa gtcaataact aatatcatgt ccaacgtgcg
    11161 ccgtggggat cggttgcgat ccgctttgcg cgatcacgcc ccgcgccgat caataaggct
    11221 tcgacgcact tatgatttga tgttttaacc tcaattccgc acccgcaagc cgggggtatc
    11281 tgctttgcgg aagaggacat cgcttacgca cggttcatgc acaaaataga gaggatattg
    11341 ctaaagaaat gcaggcgcac tgttatgggt gcgcctgcat aaatgacaaa ttcgttatgg
    11401 caatcggtgc gacaaattat tttttctgcg cgtcataaat tttgatgatt ttgtccgtca
    11461 catcaaagcc gtcgtcaaca accagagccg ctttcttttc gaaaatcatg gagtattttt
    11521 cgtttttgga aaattgtttc agaatctcct gaacgtccat gagaattttt cgggtcagtt
    11581 cggcatcttt cttcttcaat tgttcattga gatcgctttc cagccgtttg aactccttga
    11641 attcctgcgc cagcttctcc cgtttttcct tgagagccgg agaatcctgt ttcgtgttgc
    11701 tcaaatcttt ttccagagag gaaactctgt cgcccttcgc tttgagtgtc gcttttttgg
    11761 actcaaaatc cttctgatag acagcccggg cgttctgggc ggctctggag tctctcatga
    11821 ttttcgcgat atccacaaac cctattttgg ccgatacggc atccgccagg gcggcaccgg
    11881 ctgagcttat gacaaacaac agcgcaaaaa caaacgccaa cgatcctaca aaacgcttat
    11941 cctttttttt cataccctat gctcctttac acgataaagt gttatcgacc taaatatatg
    12001 ttccgaagtc cggttcatac atcgttgccc ggatatgtcc gcgcagatca gcaggcgcct
    12061 gcatccgggt cagcgaacgt ttaaaaacga cttcggccac ggccagtgca attttggcgg
    12121 atacctcgcg aattgttgtc aacgagggat agatgcggcc gattttcaga tcgtcagcag
    12181 acacctgttt gttcagggat ttggcggcgg ctgtaaacat ctcatccgtc acgcgcacgg
    12241 cttcggaggc cagcacaccc agtccgactc ccggaaaaat gtacgcgttg ttggcctggc
    12301 cggggacaaa cgttttcccc tccatgctaa cttcgccaaa aggactgccg ctggcaaaaa
    12361 tcgccctccc ttcggtgtga cgataagccg cttcggcggt acactcggcc ttggaggtcg
    12421 gattcgacag ggcaaaaatg atcggccgcc tgtttatccg gcccatcgcc ttgataattt
    12481 ccggtgtaaa ggttccgggc tgacccgata ccccgatgac cgcggtcggc ttcagggctt
    12541 ccaccgcgga caaaagatcg cgatggaagg ggtaatcatg cgcgtacggt tttttgtgtt
    12601 ccaccagatc ggtgcggcct ttgacaacca gtcctttgga atccacgaac cagcagtttg
    12661 aatgcgcttc cggcagcgac aggccttctt ccatcatggc cgaagaaacc agatctccga
    12721 taccgatggc ggcttccccg gcgcccaaaa agagcagtcg ctgccgggaa atactgccgc
    12781 cggttatacg cagggcggca tacagacccg ccagcgccac ggacgccgtg ccctggatat
    12841 cgtcgttgaa catacagtat ttgctgcgat atttttcgag cagacggaaa gcgttggcat
    12901 tgccgaaatc ttccatctga atcatcacgc cgggaaaaac gttcgccgcg gcggccatga
    12961 attcgtcgac caactgatcg tattcccgtc cgcgcacccg gggctgcggc agaccgatgt
    13021 agtccggcgc agccagcaac tccggattgt tggtaccgac atcaagggta accggcaggc
    13081 aggcttccgg atcaataccc gcgcaggcgg cgtaaagcgc cagtttgccg acgggaattc
    13141 ccatcccgtt ggcgcccaga tcgcccaagc ccaggatgcg ttcaccgtcg gtaacaacaa
    13201 tgacccggac ctttttctgc ggccagcgtt tcaggatctt ttttatgctg ccgcggtcat
    13261 ttttggaaat gaacagtccg cgaggttcgc tgaaaatgct gctgtactcc tggcaggcct
    13321 gcccgacggt cggcgtataa atcacgggca tcaattcctg tatgtgctca atcaacaccc
    13381 ggtagaaaag acgctcgttg cggttttgca gaccggaaag gtaaagatat ttttccagat
    13441 cggttgtttt gcgccggaca ttgttgatga tatggaccgt ccggtcggac ggcgaaaaaa
    13501 cacggggcgg aagcaaacca cgcaacccca ggacgtcgcg ctccgtgtca ctgaacatcg
    13561 tccctttgtt taaaccggga tcgcaaagca gcgccttgcc ccggggaatt tttacagcgc
    13621 gtttttgcgt tgaccggttt gccattgttt ttctcctgaa tcgatgaccg aatcgttttg
    13681 gtttacgggg ttgacctata agaagaatga ttgcgtgtca atataatatt caacaggttt
    13741 cccctttgcg ttgacgcggg acatgcacgt ccggggatca aatatcttct cggaatattt
    13801 cttgacatac aaaacagaaa gttcttatac tccgccacac agtaacttat caacaatacc
    13861 aacaaaattc aaaaacggag gcaatgagta tggcagaaaa gaaaggattg agcaaaccgg
    13921 tcaagctgaa gggggatctc gcggagcttt tgggcgccaa agcacttccc cgcacggaga
    13981 ttaccaaaaa actttgggat tacattaaag ccaataaact gcagaccaca aaagtcaacg
    14041 gcaaaccgga aaatgccgga aagaacattg tgatcgacgc caaactgatc aagatcatca
    14101 acaacaccaa ggtgaaaaca tcgagcggca aggtcgtgga tttcacaaaa ttaaaagaag
    14161 gacaaacgat tgacatgatg cagattgcct ctgttgtcag tgccaatgtt gagtagacaa
    14221 aaacattgaa agacaggcag gttctatcct gcctgtcttt tttatgcccc tcctttttct
    14281 tctgccgcaa cctttcgccg taccgccggt agcgacgcgc acggaattgt cttttcattt
    14341 taaattcata ttatggtttt tgaaaaaaat actgtatagt gcagggcgcc tgggacacac
    14401 aggctccaaa agactttcag attcgggaaa cgttgacatg gataaaaaag ccattttgtg
    14461 gccggtgatc atatccgtcg tcatccacat gacgctgctg gccgtggcag gcatgatcga
    14521 tttaaacgat aaaatcaaac cggtaaacgt gctgtcggtc agcatacaag aaccggcgcc
    14581 ggcgcaacct tctcctgaaa aagaaaaccc tgcccaagga aacccgtcgt ccagcgtgac
    14641 gaagcaacag aacgacccta cgcccgatga tgcctggcgc gaagatacaa ttaacctggg
    14701 cagtctcgat acgaaataca ttcattacct gacccgggta aaaaacaaga tcctccggat
    14761 atggaaatac ccgcagacgt ccttcgaacg aaatgaagag gggaatgttg tcgtcagaat
    14821 gtccattgat gcggacggca gactggccgc cgtaacgctc gtgtcatcgt cgggatcagc
    14881 cgatctcgat aacggggcgc tgggcgtcgt tcaggaagcc gcgccctacg aacctttgcc
    14941 gggatcatac aatttgtcac ggctgcatat cgttgcttct tttaactaca agatcatgga
    15001 ttagccgcgt tccaaaaccc cggacaacgg gaaaaattcc gctttcatcc cgtctgcaaa
    15061 tcaggcttta tgtcatccat gttttgattg acctgtctga gcagttgaat cacttcctgc
    15121 tgctcggatt cgggaaggga ttgaattttg tccacccggg cgttgatgta atcgatcttc
    15181 gctttcagat cggccagcag cggagcaagc tccagcggcg gtctggacgg acgcggttgt
    15241 ttaatttcct gcggccgggt ccctgcttta atattgattt cttccagata ctcgggaatg
    15301 gcaacctcaa atcccagccg ttcgcggatc aacgaggcaa gcgtatcctg cgcaccggct
    15361 tcaccgtgga ctaaatacac cggcattttt ttattctgaa aatgtcccag ccagtccaga
    15421 agctgacgtt gcccggcgtg cgcggaaaaa ccgccgatgg tccatatttt ggctttgacg
    15481 gcgatatcct cgttaaaaat acggattttt ttcgcgccct caacaatctt acggccgggc
    15541 gttccctgcg cctggaatcc gacgaacaca atgcttgcgc cgggccgcca caaattatgc
    15601 cgtagatgat gcctgatccg tccggcattg cacatgcccg atgcggaaat cacaatggcc
    15661 gacccttcca tttcgttgat tctcattgat tgctcggtcg aggacgaaaa ctttaactgc
    15721 ggcagatcca gaggatcttc accatttttc agtatgtttt gcgtatccgc atccaggtat
    15781 gtgtggtgcc gccgaaatat ctctgtggcc ttgatggcca gcgggctgtc gagaaacacc
    15841 ggcatatcct tgggcagctt ccccttgatg tccagcagat aaagggaata cagcatctcc
    15901 tgcgttctct caacggcaaa agcgggaatg atgatctttt cacctctgga ataactgtac
    15961 tgaatggcct cggccatttc attgagactg tcctcttcgc ctttatgatc gcgatcaccg
    16021 taagtggatt ccataaaaag gtagtccgcg tcgctgatcg taacgggatc ctccatgagc
    16081 aactgatgac ggcggccgat atcgccggag aaaacaagtt ttgtggaggt gccgttttcc
    16141 tcaataaaca attccaggat ggccgccccc aaaatatggc cggcgtcctg aaaattgatt
    16201 ttgatgccgg ccgcaggcgt aaaattctga ttgtaggtta ttttcttgat caaaggggac
    16261 acggcttcgg cgtccggcgt ggaataaagc ggctcgcttt tctccgtcct gccgttacgc
    16321 tgcattcttc tcgtcttcga ttttgcatcg gtttcctgga tatgggcgct gtccagaagc
    16381 agtatcttga tcagttcccc cgtcggctcc gttgcgtaaa caggcccttt gaatccgttc
    16441 tgcaccatgc ggggcaataa tcccgaatga tcaatgtgag catgcgtgat cagaaagaaa
    16501 tcgatatgag acgggtcgta ggcttcagtg tcccagttgc gtgtttcaac atccgcaccg
    16561 ccctgatgca tcccgcagtc aacggcgaat cgcgcctgat cggtttcgat aataaaacag
    16621 gagccggtga cttctctggc cgctccgaga aatttaactt tcatgggaaa tccttttcgt
    16681 caggtctttc gaggtaaaat ccgtagcggg tgaccttccc ggaaaataaa aatgccttta
    16741 tcccgcatgg aggcgggata aagtgcgctt tgtaaagaaa gtcgacaccc gtaaagattt
    16801 gtcgtttgtg tttatcttac attccggctt tcgccgcttt tttcaacttt ttggcttctt
    16861 ttttttcttt ggcggacttc gccggctttt tcttggcgtc ttttttccct ttgtccattg
    16921 atttactcat ggtttgaacc cccgttaaaa gataaaattt cattgttgca tcgtcagttg
    16981 ctgaatataa acctttacaa ctataataga agggccctgt ttcgtcaaac acaaaaatga
    17041 gggatttctt tttgttgaca acacttggat gattaacata agtttttgtg tgctttatgg
    17101 tgaacagcct atgattgaaa cccgtccgat cgaaccggtc atcgtcgccc gcaatctgga
    17161 aaaagttttc ggcgcgttca aggccgttga tgatatttcc ttccatgtgg ttccgggagt
    17221 ctgtttcggc attttgggac cgaacggcgc cggcaagacc tccaccatcc ggatgcttta
    17281 cggtttctcg ccgctgaccg caggcgacat ttttgttttc ggactgaaca tcaaaacaca
    17341 tgaccggaaa atcaaagccc gcatcggcgt ctgccagcag gaaaatacgc tcgaccccga
    17401 tcttacggtc atgcagaatt tcgaagtgtt cgcccgctac tttgatatcg ggaaaaaaac
    17461 tgccctcgcc cgggcgacaa cgctactgaa atttttcgcg ctggaacaac gccaaaatgc
    17521 ccgcgccacg gaattgtccg gcggcatggt gcgccgcctg gtcatcgccc gcgccctgat
    17581 caatcagccg gatctcatca tcctggatga accgacgacg ggccttgatc ctcagtcgcg
    17641 tcatcaggtc tgggaaagac tggaacaact caagaatcag ggcatgacca tcctgctgac
    17701 cacgcactac atggatgagg cggcgcgtct gtgtgataac ctggtcatta tggatcacgg
    17761 aaaaattctc gcgcagggct ctccccggtc gctcatccgg gaatacacgg gcggccatat
    17821 cattgaggtt gccgaaccgg atgaagcatt gcgcgcgttt tcccaagaaa agcacgaccg
    17881 ggtggaggac ctgggacatc gtttaattat ttacgccgcc aacggccatg aactctatca
    17941 ggagctctgc gaccgtttca gccagaaaag ctgcatcctg cgttccgcca cgttggaaga
    18001 tgtttttttg aaactgaccg gaagggagct gcgggaatga ccttcagaat ttccacccga
    18061 ttcatccgtg tctggcagcg caacctgacg gtctaccgag aaagctggaa gatcaacttc
    18121 atgccgccgc tgctggaacc gcttttctac cttttggcgt tcggcatcgg tttcagcgga
    18181 ctgatcggat cggtgacgta cgccggaacg gaaatttcct acgtgatgtt tatcgctccc
    18241 gcgctgctgg caatcaacat catgaacaac tctttctttg aaaacacttt tggatccttt
    18301 gtgcgcatgt actatcagaa aacgtttgat gcgatgatgg ccacgccgct cacggttgac
    18361 gaaatcgtga ccggagaaat cgtctggggg gcgaccaaat cggtcatcgg aacgatcatc
    18421 atgctgattg tcatcggttt cttcggattg atcagctatc cggcaggatt gctgatcgtt
    18481 cttgtcgcct tcattggcgg ctttgccttc ggctccgtgg ccatgatctt caccggcctg
    18541 gtcggcaaga tcgaattatt caacttgccg gtctttctgt tcattacccc gatgttcctt
    18601 ttcagcggca cgtttttccc gataagcgga ttgcccctct gggcgcaata tttcgccttg
    18661 acgctgccgt tgacgcatct ggtcaacctg gtgcgggcct taggcctggg agcgttcaac
    18721 gccgaagcgc tggccggttt ttgctacctg attattttct gtctcatcac ctttccgctc
    18781 gccctttccg tcatgcgcaa aaaactgatc aaataatggt tgccaggaaa aaacgaaata
    18841 ctgcttgact aaaaattctg agtatgcgca acttaatggc ggcgattccg tatatttttt
    18901 acaaacggat tacaaccatc aaggattatt catgtcctga tggttttttc tttaaagatg
    18961 taaggcagac aagcccctca ctttttcctt ccttctcaac caggagacgg atgggggtga
    19021 catggatgct ttgtaacacc ccatcgggga atgaaaaaaa gaaataaaat cagaaagggg
    19081 ataaagagat gaacaagaat ttatacgtcg gcaatctgtc ttataagatc acaggcgatg
    19141 acctgaaatc caatttttcc gaagcgggag aagtggtctc cgcgacgatc atcaaggata
    19201 aattcagcgg ccagtccaaa ggattcggtt ttgtcgagat gaaaacggaa gaaggcgcag
    19261 ccgaagcaat taaaaaattc cacggcggca cgcttgacgg aaaagaaata acggtcaacg
    19321 aagccagacc gaagaaagag ttcagcggcg gccatcgggg aggcggcaac cgcggcggag
    19381 gaggaggatt caaccggggc ggccgcaggg atggaagata ttaatcattc tcgcgttgaa
    19441 aacgcataaa acaagcagct ttggacctga tcccgcattc tagacacatc gggacggcca
    19501 gaggcgcaaa ataaaaatcc agaggagcag aaggcatttt gaaaccttct gctctttttt
    19561 tggtgtgccc atgaagggag ctatgcagaa ggtacgcaaa acttggatac aatgacccgg
    19621 acaatattat gcatatgctg atgcacggct tttgcgcttc ggccgggata gtttctgaaa
    19681 gtaatcagca caccgttgag cgcagcaaaa aatgcatggg acagaggcct gaccggttcc
    19741 ggcaggttca tttttctgaa caaaacgtcg aactgatcaa agatacggcg ttcagaggcg
    19801 ttgagctttt ccaccatgtc cgcggagagc gaaccgtcca gcatgaaacg cgtcatcatg
    19861 cggaaatact gatcgttatc gatgagataa ctcaaatagg catcggccac cttttcgatc
    19921 gggttttttc tgctgttttt gatcatcctg tccagaatgg ccgtaatttc ttccgcgccg
    19981 tgaagaaagg cttccacaaa aagggattgc tgatcgggga aataccggta gatggaggcg
    20041 tgggaaatgc cggcctcccg ggcaatgtcg cgaatgctga ctttgttgaa cggttttacg
    20101 gcaaagacgc gctgggcagc ttcgacgata acgccttgcc gcgcccgttt ttcgttattt
    20161 ttcagttcgg caaaagtatt tttctttttc atatcgattt ttttatacca tatcccgcaa
    20221 aaacattgca gtaaaaaagc ttgacttatg taaccagtgg tttgtaatat aaccgttggt
    20281 tgagaaagtc aatcacaaat aagattatcc gggaggttat atgtctctat ctgatcgcaa
    20341 caatccctat acgtttgatg attttttaaa gtggcggcaa ggcgttgact actaccggga
    20401 tgatccgttt atccagaagg tggtacacca ttttaccgga gacatccggc agcaggttga
    20461 cgccgaagcg cgcgcctgct ctcaaaaagc ctctttccgg tggcgcgatt ttgccgacag
    20521 cattgcccgg cccgaaaaga ggcccttcat gctgcactac gacgggcatc ataaccgcat
    20581 cgacagaatc gtccgcccgc atgaaacgga aatcatggaa aaggaaattt tttccgaagc
    20641 attctttgcc gacacaacgt cgccctgggt caaattaatt aaaatgtacg tcatctacca
    20701 gaacggcgaa gcctgtatcg gctgccctgt cacctgcaca gaggggctgg taatgcttct
    20761 tgaaaaatat gccgactcgc cggagacgaa agaaattctc aaacattgta aagacggcat
    20821 caacggcgat tttgccatcg gcgcccagta tctttcggaa atccagggcg gttcggatgt
    20881 gccggcaaat gtgctggaag ctgtcaatga aaacggcacg tggaaactct atgggacaaa
    20941 atttttctgc tcggccacac atgcggacta tgccgtggtg acggcaaaac ccgtcggatc
    21001 ggaaaaagtc gccctctttg tcgtgccttc ctggcttccg ggcaataagg aaaaagaaat
    21061 gcgaaacggc tacaccgtcg accggatcaa atggaaaatg ggcaccagcg aactgaccac
    21121 ggcggaactg acgttcaacg gcgcagtcgc ttatccagtg ggtcctctgg acaaaggcgt
    21181 tgccaatgtg gtcggcattg tcctgaccta ctcgcgcctg accgtggggc tttccgccgc
    21241 cgcctacatg acacgggccg tccgggaagc gaagaaatac accgaattcc gcaatgcctt
    21301 cggcctgacc atcggccaat tcccgctggt cgccgttcag gtgaacagaa tcgatcgcat
    21361 ggccaaaagg acaacagctg gagcgtttaa actttatcgg gattttctga cgctggaagg
    21421 aggactcaaa ggcggactgg cgacggatga gccggaagcg gtccggaaga atcgtttcaa
    21481 tatccgggaa ctcatcatgc tgcagaagat ggccgccgcc tgggactgca cggatgtcat
    21541 tcgcgagtcc atgtccattt tcggcggcca cggagtgatg gaagatttct catccctgcc
    21601 tcgtctgtac cgcgactccg ccatcaacga actgtgggaa ggtccgcgaa atgttctttt
    21661 gacacagatt catcgcgacc tgcaaaaagt cagcggctgg tataagcctg cggagtttgt
    21721 gcgtcatatt ctttccggct tgaatgagtc aacgattaaa gaattggccg acgagatcac
    21781 cgaactggtg gcgtacccca atcttttcca gccggatgaa aaaaccatgg atatctgccg
    21841 gcgatgggat gcgttttgcc acaggttttt ccacgcctat caggatctgg ctctgaaaga
    21901 ggtggaataa ttcaccggat tgcttcttaa gcggatttgc acaacgctca caattcttaa
    21961 tggattgcag cttaagcatc gcggatgatc agatgtcccg acattatccg aatcggacgt
    22021 cagcctgaaa cttgatcagg acagagatat gctgaaaccc atgaactgcg cggcgagaaa
    22081 aagcacgtag atggcaatca tcaccagcgc ctccagacga acaaattttc ttccggtggt
    22141 gacaaacatc tgcagaagaa cgcccgccac gatcagcatg ggaagatcgc gctgcacgat
    22201 gatcggttcg accggaagag gcttgatcag gcacagacaa ccgatgacgg ccaatccggt
    22261 aaagatgttg gatgcgtaga tctcgccgag cgtcacatcg gcctgacgcc tcaataccgc
    22321 gcccaaggcg atggcgagtt ccggcagcga tgtgccaaac gcataaaagg tcaccccgat
    22381 gagaagatcg ttcatgccca gccgccgcgc aagcattgtt ccattttcca caatccagct
    22441 cgaccccagc acgatcatcg caatgcccgc cacaaatata atgccgtgca gccagggctt
    22501 tgccatgctg acaacgggag cttcctttct ctgctgacgc gcctcatcga tggtgccctt
    22561 caaatacgga ccgtatgcca tcagcagaat caggccgtcg atgcggctga tgactccgtc
    22621 catggcaatg accagcaaca gagcggcgga aagaatcatc cagctggatt ctctttcacg
    22681 aagggtcgga ccgacgatga tcggtctcca cagggcgcaa atgccggcca caaacgtcag
    22741 ggtcacgaaa ttggagccga caacattgcc gaccgcagcg gcaggctgtc cgcgcatgga
    22801 ggcaaacagt gaaacgcaga actcgggcag agacgtcatc accgccagca acagaacggt
    22861 caccaccagc ggcgataccc gcagccaccc cgccagaatg ggaacgcgtt gcaggacgaa
    22921 ttccgtgccc gcccataaaa gcacaatgcc cgcaatgaac aacaaaaccg aaagcgtcat
    22981 cggatacccc tgaactgcaa tcacgcaaaa ctgcccggcc cgtcattgca gattccgccg
    23041 gatcaaatta aaaggcttgt ctcgcggtca ctccttaata taatttgatg gcggtctgca
    23101 tccctttgct tgaagaatca atatattcgg tgaatttttc ggcgatggtt ttgaccgtgt
    23161 gttcgaattc atccacatct ttgtccagaa gcgtcatgcg gaagcctaaa agcggtgtaa
    23221 agaacgatgt cagcggaaca acacagatcc ccgtcgccgc cagcagataa taaacaaatc
    23281 tcttgtcgtg ttcgatgggt tctttgacga gattttcgac gaattccctt atctctccat
    23341 tttttatatg aagcttttgg ttgctgttga gaccggattc gttgaacacg attgtcatat
    23401 aaaaagcgcc gtctgtttta ttgaccatca tgcagggaac atcttttaaa atgttgtagg
    23461 cgatatccga aagcttttcg taatgcctcc tgcgttcctg gaggtattta tcatactccg
    23521 gatgcgtcat gattttagga atcgccagtt gcggcaatgt tgtcgaacaa acctccgaca
    23581 ttttctgatg gaggatggta ttgataaagc ggtcgaagat cggatcttta ccggcattgt
    23641 aaacttccat ccagccgcac cgtgagccgg gccagggaag ttccttggaa attcctttca
    23701 tgctgatggc cggaacggag cctacgatat ccgaaagcgg tacggtgttc ttgccgttgt
    23761 aaacaatatt gatgtaggtt tcatcaaaga caacgaaaag atcgttttct ttggcgatcc
    23821 gtacaatatt tcgcaacgtt tcttcaggat agacaaagcc ggtgggattg tccgggttga
    23881 tgaccagaat gccgacaatg gctttgtggc ttctcacctt ctgttccagt tcgcgcatat
    23941 ccggatgcca tccgttgtat ggattcagcc ggtaggtatt gggcggaaac gacgcgtgca
    24001 gcacttccgc catgaagtgc gtggaatagg tcggctccgg catgatcatg cgggcgtcaa
    24061 cctgaatgga gctgtaggcg cgggcaatgg catcgcccag accgttgaag aaaatgatat
    24121 cttcgggggt aatccgcaca tttccgcgcg cattaagacg ctcggtgaga aactcccggg
    24181 tggcgttaac acctttcgtc ggggaatagg caaaagactc atcatctttg atgatttcaa
    24241 agaggacttc cttcatccag tcgggaattt tttctccctt ttgcaccgga tcgccgatat
    24301 tttcccagat gatatcaacg ccgtattcct tcagtttgtt ggcaatcagt acgatattcc
    24361 gtatttcata agtcagcccg ctgccgccgc gggaaatttc aaaacgcata aatattttaa
    24421 ccccttagat gatatttgta tttcataata catatttgtt taaatttcca gttttttatg
    24481 ctatcggagc accaaacgaa acgcctgtcg ggcgtaaaaa taaagaggac aatatgacgg
    24541 aagaaaaaag tttttctgaa atgcttgatg aaaccagctt aaaaccccgg cgtttttccg
    24601 ccggagagaa aatagatact atcatcgtaa aaattacaga ggaatggatg ttcctggatc
    24661 tgggagcgaa aagtgaaggc tatctggata aaaaggaact attggatgaa aacggcaatc
    24721 tcaccgtgaa ggaaggagat cccatcacgg cttattttgt ctcttctcgt cacggggaaa
    24781 aactttttac caccaaactg ctgacgagca agagtgttga tgattttatg ttcaaagcct
    24841 ataaaaatca gattcccgtt gaagcgactg tcgagaagga aatcaaaggc ggcttttcgg
    24901 tcaggattaa ccagaatgtc agcgggttct gtccctactc gcagatggat gtcagaagaa
    24961 tagatgatgt cactgcctac gtcggcaaga aatttgaatt tattgtggcc gaatattcgg
    25021 aaaagggtcg caatatcatt ttgtcacgtc ggcctttgct ggaaaaaatc gaacaggaga
    25081 aaaaagccgc gttgaaaaat tccctgcaac agggaatgac ggtgagcggc gtagtgacga
    25141 gcgttcagaa attcggtgct ttcgttgatt tgggcggaat tcagggattg atgcctgttt
    25201 cggaaatggg ctggggacgg gttgaagatc ccaaagtact ctactcgccg ggcgacaaga
    25261 ttgaagccgt gatcatcaat ctggactggg aagctaatcg catcacgtta tcggctaaag
    25321 cctctcttcc caatccgtgg gacggctttg tgagaaaata caccgaaggt agcctgctca
    25381 agggcaaggt aaccaacctg accaatttcg gcgcttttgt ttcgctggaa gacggggtgg
    25441 aagggctttt gcatatttcc aaactggcgc gcggcaaaaa aatcaaacac gccggcgatg
    25501 ttatgaaaac cggcgaagaa ttggaagtga agattgaaaa aatcgaccgc gagaacagaa
    25561 aaatttctct tgatctgacc ggaagcgaca aagatacttc agctgcttca ggaaatgaag
    25621 atgatttccg cggttatatc ggcaaatcac ccaagacgat gggcacgctg ggcgacctga
    25681 tcaagaagaa aaaataaatt ctgggttgaa aaagaaaata cctacaagtc cacattttta
    25741 tagaataaag aaaattgagg attactagtt gaaaggtgca acgcctgata tgaggtgtgg
    25801 cggctttttg ttgttgccat taatgccgtt gtcagttact atttataata tcatcattgt
    25861 caatatatcc ccaaagataa aagttatctt ctactgatac gtgaacaaca tgccaaaaaa
    25921 taccttttcc atttagagct gaaacttttg agtcaacaaa tacttttttg cagttggctg
    25981 gcacagttcc gacaactttg cttttattat cagctttttc atatattggg actgattcat
    26041 tttttaaact cacataatac tttttataat ctggtgattt tgaacgtttt atatctaagt
    26101 gttttgttaa aattaaatta attgaagagt cgagaacata ttcagctcta tcttttatac
    26161 cttcctcatc aagtttttga aaatctttct ttacaatcca atctgattct ttgttttttc
    26221 gatataaaga tggagtaagc cgccaaacat tccaataatc gacccggcta attcctgccc
    26281 ttaacagatc catatctaga cggtcatgat catagacaat ataatctgtc ggttctttaa
    26341 catattcatc gatatatttt ttattgcaaa cataaaatgg agctttcatt cccgttagca
    26401 taatagccat agtacccatt attttttctc tttcatctcc atatagactt atgtcatagt
    26461 ccgtttcaaa ttctacataa atggctttcc tgcaattgat aaggacatta acataatctc
    26521 cctttgagaa atcattttca gcagccataa gataatcttt tgcctcacct ttattgatca
    26581 aatcaacaag gctgatggtt acaaattctt tctcgaatac ttggatagaa acttcttcga
    26641 agaactcctt tacagtttct ataagaccat gtgtttctaa tttagaagga gataaaccat
    26701 agtgcttagc gttaaccctg agcttgttaa gagcggttag gcgaagctta aagggaagtt
    26761 cttttggaga aagtttttta tttattaggt cgaaatattg attaaaattt gaattattac
    26821 caatactagc atctatatgt tcggcaacag caagaagaaa tatttcaaca gaatcttgta
    26881 atagaataac gccgatggaa agagaaagtt cattatcaga atttaaattt tcaagtgata
    26941 attgataaag tttccgagag agaagtattt ttgtaattat atttttccgc attatttact
    27001 ccttaacgga tttttataac aattccctat ctttcttatg acactcttaa tgcaaaagga
    27061 ttgcttaata attacggatt gtaacctagc ataaaattat atttttgaaa aatgaggatt
    27121 tcaggaagca agacttgaca ctaaattatt aggccaagtc tcctgcggag cgataaactt
    27181 aagatttgtc ttccagtacg attaaggcat cggcgaccac cggttcggcg ccgttgatga
    27241 taatcggatt gagatcgatc tcggcgattt ggggatgcag aagagcgatg ttgcccagcg
    27301 cctgaatgat cctggacaaa ttgcttaatt tcaccttcgg caggccgcgg aatttatcga
    27361 gcagtttacg ggcattgatg tcttcgaaca tttccagcgc atcggcgctt gtaaccggcg
    27421 cgaggcgcat tgtcgtgtcc ttgaaaactt ccgtgaaaac gccacccaga ccgaaaacaa
    27481 cacccggacc gaacccggca aagcgcgtca taccggccaa aaattcgcgg ctgccgctga
    27541 gcatttcctg caccagcacc ggcgcagttt ttcccgcgtt cttttgaata ttttcaaatt
    27601 cttttttgag ctggttttcg ttttccacat tcagcgcgat gagtcccttg ccgcttttgt
    27661 gcatgatttt ccaggagcag gctttgagcg ccaccggata gccgattttt ttagcggtct
    27721 tcagagcgtc ttcaaccttt gccgccagcg cttccttcgt taccggcaca ccgtagcagg
    27781 caagcaactg tttcgcctcg tattcatcca gcgccttttg tttgttgtcc agcgccttga
    27841 ggatgatctc cgtggcctct tttttgattt tcggcaaagc ggcttctttg cggggagcgc
    27901 gctctttgat ttgtttgtac aggtaaagcg agcccagcgc cctcgcggta ttttccggcg
    27961 aataaaacac cggcgtgttt gtatcctgat agcccttgat atagttgtct tcatgatcaa
    28021 agaaagaaga tatca
//
LOCUS       DAOOLB010000023        27160 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_1594_length_27160_cov_36.657886, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000023 DAOOLB010000000
VERSION     DAOOLB010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 27160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27160
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1594_length_27160_cov_36.657886"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="PLF77_04730"
     CDS             complement(<1..804)
                     /locus_tag="PLF77_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC08192.1"
                     /translation="MAQKKDPLYLTWLGTAGVLIEDDHDGILIDPYISRPGLLSVVLG
                     LSLKPDRDAIEKWLTRPGTKNIRAVLVSHSHFDHCLDAPFFALKTNALFIGSKSALNA
                     ARGVPLDESGLKEADIGRDIKIGAFTLRFIESAHGPAFLGRVPYPGTIDRPLTGRHSA
                     RDYKLGQTYSILITHASGTILHHGSAGFINGMYDGITSDIVLLGIGGRGHTQTYLENV
                     PLKLNAKIVIPIHFDNFFVSLDRKMKNLPGVNFQEFLTTAGKYGNRFQLK"
     gene            complement(844..1608)
                     /locus_tag="PLF77_04735"
     CDS             complement(844..1608)
                     /locus_tag="PLF77_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HPC08193.1"
                     /translation="MTNQQAHQKISTALMRDIFFHKLQPGEKLPSERELSGSLNVDRT
                     SLRVALKQLEAMGLLDIRRGDGIYVKDFLKHAGIDFFRTLFSRIETLEDELAADLFVI
                     DEVWEWWIIMFPEMLKLAAQRASARDLKALSDLMDSELNNLDHRDVLIELWLDEQDMI
                     ARAVNNMVLILIFNSSRPLRRSMLRIYHDAVNRENMEQFIISKKSLLMDFLMNSDKTM
                     LADVEQYRDILIAQRQVARSRLIQSGPSPGKTGKEK"
     gene            1906..3309
                     /locus_tag="PLF77_04740"
     CDS             1906..3309
                     /locus_tag="PLF77_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPC08194.1"
                     /translation="MRDVYVIGAHTIKFGKYIEKSIKDLAAQTVISCLKEAHLEKKDI
                     QALWFANSGWGYSKGQDCIRAQVALRPIGIDAIPMTNVENACAGGSTAFHHAWLGVAG
                     GLYDITMAVGAEKLYDNNKMKVFAGFLSGLDIENLSSIIANISFYGMSEQDKKDMQAH
                     VAKYAALAKKNAAIKRKKKGVGSRFSDLLDMFKVYLRLSDAVGSETVRKMRKLFSGDH
                     SPFMDVYGFAARQHMKKYGSTIEQLALIASKNHFHSSLNPNAQYTFTITPEEVLADRM
                     VSWPLTRSMCAPIGDGAASAVLCDQATVKKLGLSSQAVKIRASVLGSGRERSFDDTDI
                     GERLSKIAYEKSGLGPGDMDFAEVHDASAFGELHQAEALGFCGEGEGGRLAESGATRL
                     GGKIPVNVSGGLESRGHPVGASGLAQIHELVTQLCGKAGKRQVEGAKIAIAENGGGAL
                     GQEEAAMCIHILEGPSK"
     gene            3363..>4129
                     /locus_tag="PLF77_04745"
     CDS             3363..>4129
                     /locus_tag="PLF77_04745"
                     /inference="COORDINATES: protein motif:HMM:NF004196.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC08195.1"
                     /translation="MDLNKKFPEKRVVITGAGSGLGRALALEFARRNWRIAIAEINDE
                     RAAESAKMVREQGGIPLTIHCDVTLPRDMENVLTIVRREWKGVDILINNAGVASAGWF
                     ERIPLEKWDWIIGINLMSIIYGCRTFIRYFKESGTGGYIVNVASSAGIASLPEMVSYN
                     TTKAAVISLSETLRIELSKINVGVSVVCPTFFKTNLMDQFSSPDERQRQLADTFFGNA
                     KITSEQVARHTVASIEKNRFYVITQADGRNMWRMKRW"
     assembly_gap    4130..4229
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4720..6402
                     /locus_tag="PLF77_04750"
     CDS             4720..6402
                     /locus_tag="PLF77_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPC08196.1"
                     /translation="MGIAGDIVIIVMAALLGGLIAQMLKQPLILGYILAGVVVGPYTG
                     GVTISDIHSIEKLAEIGVALLLFALGLEFSLRELRPVRAIALFGTPVQIILTTLYGFG
                     IGRILGWSWTSSVWFGALISLSSTMVLIKTLEKQGMMGTLSSRVMIGVLIVQDLAVVP
                     MLIILPQLNNVQDGMAILLVAAVKAAIFLTVMILVGTKIIPRLMRYIANWNSRELFLI
                     TTTTIGLGIGYATYIFGLSFAFGAFVAGMLLSQSDYGYQALSDIIPLRDVFSLMFFTT
                     IGMLLDIRFLLANWEIILLLVGLVMLGKAVILAVLSRVFGYHNVIPLAMGLGLSQVGE
                     FSFILGRVGISTKSITGEFYTMVLTMTIVTMLLTPFISGLTTPLYAMARRWMKPLKLE
                     PLNFPSKGLKDHIVIAGGGRVGMYVANVLQQKNIPFVILEYNSRRVHEVRKSGFPIIF
                     GDAAKTIILEAADLKRAKLLLITTPVAVISLGIVAHAQRINPGIRIIARAEGEEQMKA
                     LYTKGVTYVVQPEFEASLEIVNQTLLNLGIPAEQFKTFTEDARQELHRPLSV"
     gene            6450..8303
                     /locus_tag="PLF77_04755"
     CDS             6450..8303
                     /locus_tag="PLF77_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPC08197.1"
                     /translation="MKLNLKTIIRKCLGFVNFRSMTKATLWWMWPRRHRVFFIFEGRE
                     FTYGDVYRQAQRYAHFFLSIRKKLVDSGKLASDQRMAFGVYMENTPEFLFASFGAALS
                     NSVLFAINTGFRGDILGKVMEQAGISFLIVNDSTLKEVEGVVKNVNIIGPENIYVVRE
                     GEKSLPDGFQWLDKAVEEAQQIDVPNRYRVRVDNFSPVIVIYTSGTSGMPKGVPCTHI
                     KMFGAGFVVQSAVHLNRRDRGYISMPLFHSNAWYIGILPQMIAGGSFVLKRRFSASAF
                     EDDMLAHGVTFLNYVGQPLHYIIDALERKYGSGEAVEAALAKHPRNRFRKAYGNGATV
                     NDRIKLKRYLGMDHIYEIYGSTEAVITTANCPGDPVDSVGRVDGSVRILDEEGKECEP
                     GMVDEKGALLNYDKAVGEICRKVGDNNLRFDGYYGNAGATSQKFRNGWYHSGDLGHIR
                     IIKGKRYMFFNGRTDDWIRKDGENFSAENVLDFAQKIPCVDCAIAYGAPCEVADEKVM
                     ITVQLKEGADFNPDEVHEWFIQQQKEGGMDPKWMPDYIRVIEKFSVTDTQKILVRPFK
                     RENFNIEINASMRIYFRRRGDTAYRLLTPDEFRKIREDFKTNGREAILASC"
     gene            8371..9009
                     /locus_tag="PLF77_04760"
     CDS             8371..9009
                     /locus_tag="PLF77_04760"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPC08198.1"
                     /translation="MLQKIIIATIIFLFSLSLCWAAEGIKLPAPQMDGGKNLMNAFKE
                     RKSERSYSAKKIPLNVLSNMLWAACGINRPEAGKRTAPSAMNRQQIDVYVATADGLFL
                     YEAKGHLLKPILNQDIRPLTGKQSFVAQTPVTLIYVADYKKMGNISNEEKAFYAATDT
                     GFISENVYLFCASEGLGTVVRGSIDKEALAKTMKLGDHQKIVLAQSVGYPKE"
     gene            complement(9179..9439)
                     /locus_tag="PLF77_04765"
     CDS             complement(9179..9439)
                     /locus_tag="PLF77_04765"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HPC08199.1"
                     /translation="MEETENKLHLEYPCAWIYKIIGTDEDEMRMAIDEIIQDRNCLVT
                     FSRSSRNNSYRCLNVEVIVESESHRQTLYELLKAHRAIKIIL"
     gene            9969..11885
                     /locus_tag="PLF77_04770"
     CDS             9969..11885
                     /locus_tag="PLF77_04770"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HPC08200.1"
                     /translation="MKSFLANHYAIKDLNAEINNQVVDQMIKRLDPSKTLLYDSDVQN
                     LRPVLRRAFSSMQSGDCALLKPVYDLLVTRARENEKIIRRILGSNYRIDEDAELYIDV
                     KKRPYVKTAAEKYDLLKKVVQFQIENDLLSGVTLAEAKKQQVHRYELQTARVLERRPE
                     KLIEYAAEAFAQALDPHTNYLSPDNLEDLKIHMQLSLEGIGALLSSDNGFTVIEELIP
                     GGSAEKSGLLKPKDKIIAVAQKRAKPVNVIDMDLRDVVKMIRGKKGTQVTLMILRQSE
                     RTYRFDITLTRDKVYIKDQEAKVDYQTRMVNGKKYSFAVIDLPSFYGDEKENKSSYED
                     VRALLMEARQRGVDGVVLDLSRNGGGLIGEAVQIAGLFLGRGPVVATKDNSEKLTVFV
                     NGAATSEMKSRKNKIISFPVEDRALYTGPLVVLTSRLSASASEIVAGALQDYRRAIIV
                     GSDRTFGKGSVQTLAPLPRDLGGMKVTTGLYFLPGGKSTQKTGVPVDVRLPGFFVLED
                     VGEAELEYPLPAQAIQPFIKASSNSALLWTTVDHPMVAKLSIKSQARIAKDPKFKEII
                     QKNKEAAEKKGLIRLVDLREERKKEGGSNKKETPAELKQKVRDQYAPYVNESINILSD
                     MMTAKAALPMAKAL"
     gene            complement(12090..13121)
                     /locus_tag="PLF77_04775"
     CDS             complement(12090..13121)
                     /locus_tag="PLF77_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08201.1"
                     /translation="MPGRIPLTMDCQPSFFSAIDVEGYVHRTAVAENNHRIVGVVSMS
                     KRRLYLNGVPEDFGYIGGLRIDSSLRNTTILVRLHNILKKWHQEGFGVRFYLSAIPYD
                     NVGARNIMTSGRAGFPDCTDMGVLYNAAIPLFRRRLPRPQKGLRIVRGTAVGADAIAE
                     FLNRNGSRRQFFPVYTADDITADNGILRGLHPDDFYVAVEKDRIRGAMACWDQLPFRR
                     MLIADYPWYLHWSKRLSAPLAGILHTAPMPEPGEPLRNIVAACIAIENNHPKIFNLLL
                     QTILHDHYDTGKAFLVVGLMEKDPLISALKSRLHISTRTCIYMMSGNGYNILSEMDER
                     PPYLELGAL"
     gene            complement(13339..13842)
                     /locus_tag="PLF77_04780"
     CDS             complement(13339..13842)
                     /locus_tag="PLF77_04780"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HPC08202.1"
                     /translation="MSWYVIHTKSRHEHKVNDGLVQKNLTTFLPEVEVWSKRKDRKKK
                     IMVPLFSSYLFVDTDLNNYETRLDILKTPGVVRILGKKENSEPVAVPDDAIRAIQKFL
                     NKKTEIFSLQYPQKGEWARITGGPFAGLEGIVVKSDLNKEMFVVSIDLLHRSVAVKVE
                     GFKITRL"
     gene            14106..15353
                     /locus_tag="PLF77_04785"
     CDS             14106..15353
                     /locus_tag="PLF77_04785"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPC08203.1"
                     /translation="MERTHNISDLEKTEYLKINLGKNILPLSAAENKYMPGAGPIEII
                     ISYKRSTAVARGIECFLKAADYYNLFSSRLIMIGENTFALQYCTDGIVINILPPVHAR
                     FDDVRIDDLREKMTHVQTLPGHPLLALTLIPVNDGHFVGFSCSHAVADGISCILFLYA
                     WNCLIEGKPFPRPSAQRLFSGNPIPSDHIEKDWMPPLSALSEEIQNRVRRGAIKTYKT
                     TEYFADEFLAEMKHQAQANNREFVISNNQIMTAWLLKKYHHHILPHTDKIVIRTPVDL
                     RTVHPGVDPMYIGYAVINSFSEFRKKEIDHMSVVETACRLKESIHHTRTKRFIQEATY
                     LSKYGIEIREEVLKQFPPYNPDTDIVSTNMTHLSDLESLFLGPDAMRIEHIGVASVKT
                     SFAMVREKNSQMSAQITSRYPLI"
     gene            15375..16694
                     /locus_tag="PLF77_04790"
     CDS             15375..16694
                     /locus_tag="PLF77_04790"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC08204.1"
                     /translation="MLSSASRYNIRFARPEEIRDIIELMRPYNMHHIPSPEMPPLDDK
                     RFIVVEQNGQLLGAAGYTFVSEEIGKTTLMAVRPDCARMGLGKILQTRRMQILRGLGC
                     TKIITNTDRPETIAWYKQHFGYREIGKLPKIHSFSLEHVPEWTTLESDLTTIDVPSPV
                     QGKLLMNAALTGMKHTRSDNPYLPVTPAEIAEAALEAGKLGAAIVHLHARDVDEKPTP
                     DPSIYAEIISRIREKNADLIICVTTSGRNWSDLQKRSAVLELPDDVKPDMASLSLGSM
                     NFPSGASINPPEIIRSLALRMMERNIKPELEAFEVGMVEYAKYLIREGVLRQPVYLNL
                     ILGSLGTMSASPDNLRYLVSRLPAFSTWAASGIGRYHSKVQDMAAAMGGGIRTGLEDS
                     SMHLDADKRIPAKNKDWIKRAVEAASLNGREIATPNDVRQWLDLRKS"
     gene            17150..18412
                     /locus_tag="PLF77_04795"
     CDS             17150..18412
                     /locus_tag="PLF77_04795"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HPC08205.1"
                     /translation="MKQKWIATAHKGLRYFEHDTRKHGKRFDRYYSIRFRVDRRLHTF
                     GVGWVSDGIPEAVRQEDPGLGFEEYCLKLLRQYKVNAKTGTGPTSPREKRLIEKEKEK
                     QVQEEKARLEKENMTFGYYFEKKYFPTYQTGRKKSTSRKAQEHFQNWINPVIGNTPLK
                     DIKPFAIEKIKKNILDGGKAPRTLQYILATIRHAWNTARINGLVVGDSPTKNVQIPKI
                     DNKRVRFLTHKEAENLLKSLKEEDVLTYYISLLSLQTGLRFGEIAKLKWGHIDLDKGI
                     IEVVDPKGNPTRIVYMTGKIKAMFDKMERRKPDDYVFFKDGAQLKDTPKVFFKVVDEL
                     GLNDGVSDSRRKVVFHSLRHTFASWHVMAGTDIYTVKKLMGHSNIAMTERYSHLAPEA
                     LQNATRTLEKAINKAGHKKIGQVVKFQK"
     gene            19073..19231
                     /locus_tag="PLF77_04800"
     CDS             19073..19231
                     /locus_tag="PLF77_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08206.1"
                     /translation="MNHNEIIERKIFLKRQLIVIIKKEIKKLEGSLKHIGEEDDRRSM
                     DQNNNVNV"
     gene            19188..20012
                     /locus_tag="PLF77_04805"
     CDS             19188..20012
                     /locus_tag="PLF77_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HPC08207.1"
                     /translation="MIGVQWIKITTSMFDDEKIKFIDSLPERDTIFYIWIRLLVLAGK
                     CNAGGYIFLTENIPYTEESLSSVINRPLNTVKLAMSTFLQLGMIETDSKGIYLLNFNK
                     YQNIEGLERIREQTKKRVAQYRERKKLLEYDGGVTLPVTLCNGTEKEREIDKIYIQNA
                     LTVLTYLNEKTGKRYRIKKYIMARLRDGATIEECQSVIDAKIKDQFFIDNPKYLNPET
                     LFRPSNFDRYKNETIPFKTESPKTKINSCSRCGSQVPPQDMTANGCIYCEGKEASA"
     gene            20009..21337
                     /gene="dnaB"
                     /locus_tag="PLF77_04810"
     CDS             20009..21337
                     /gene="dnaB"
                     /locus_tag="PLF77_04810"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPC08208.1"
                     /translation="MTVNKVLPNNAEAEAAVLGAVLMENRALNECLSIIGPDDFYSDR
                     NRLVFLAMCELSDKGKPIDSIILSNHLKARGQLDRIGGQLYISDLLDSTISSAKVAHY
                     ARIIKDNSVLRSIIKAAGDMADHAFSINGEGPGIVLDEAMRSLMNISIDRKRHDVRDS
                     KDISKTTFKAIEERHENTNFISGLSTGFNELDCWTSGLQESELIIIAGRPGMGKTAFG
                     VNIVENVVLSGVPAVVFSLEMSSESLMTRIFSSLANIDSRRLRCGRISDSDWPRLVEI
                     VNDISAAPLFIDDSSVLTPMQLKAKARRLKIEKGIGLIVIDYLQLMTGTGKTESRERE
                     IAEISRSLKALAKELHIPIIALSQLNRAVENRSKKEPSLADLRESGAIEQDADVIIFL
                     YRDEVYNKSEVNPERGLAEVSIAKQRNGPTGTFKMRFSAELTKFHDFGRQ"
     gene            21432..21992
                     /locus_tag="PLF77_04815"
     CDS             21432..21992
                     /locus_tag="PLF77_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPC08209.1"
                     /translation="MNDMKTQEQRTEFIKLRAEGKSYTFIAGALNISKSTCTGWEREF
                     KDQISQLKREELNELYDSYYMTREARIKHLGDTLVNINTVLDSKDLSTMSPEKLLDFK
                     LKYTEALKAEYMDTDPPQRLGANFKAQDILKALGNLLDRIRAGEVSMEQAGRESLVIS
                     NLLKAYDTVELQTKLKELEAIIGGRE"
     gene            21992..23437
                     /locus_tag="PLF77_04820"
     CDS             21992..23437
                     /locus_tag="PLF77_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08210.1"
                     /translation="MKTDINNLLKKGKLTGQEVGQLMIQDLVATYKNYLEGKGDEGFL
                     TQAETNTLVNGLTKSQDIKRYREFRGVHEYIVNAARAFVIHEEIMEEHLWKINYIFNE
                     MRHAEEENFQAIRQPRIMTQKQYDELKREDFEEKMTWTYSFEMLLFDALEYYINLHNE
                     GEKTPFDKHFAAAKKQPIKNPRIKADYYESESEDKTAADTATMFDVLEYAKGFYSSLE
                     TDSQKTLFEFTEDFPGLYKDLWSKLTAMKGLNFLKDIPKKKYFDDALISVRELYENNI
                     LNFRDFVGTFHPDGCGGGVAVLQSHCFYPRRNIDEHGYYRAPEPYWRKHFMAEAILSE
                     PLRRTITRWIALFSEHLKECFVIRTAFLLIGEFTGVPQISTLIDGIDGEIDRLTKRGE
                     LVNQIIENAHDLILSRYGALSNERSVDELRSEFKNTFPLIDMKYLMPSEDAIEEARQT
                     LDFTMIQNDAEQLYAILKNEEVENTESTLAC"
     gene            complement(23543..23857)
                     /locus_tag="PLF77_04825"
     CDS             complement(23543..23857)
                     /locus_tag="PLF77_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08211.1"
                     /translation="MKKIAGYIFILAVAVVFAAYPLQSASAQSGEYYSGPYQTGGYFG
                     IWGGYTIAPDLTYEYCDDWDCDEYDLNIDIDETAVFGAKLIDKNLLLVVSSIRNVLLR
                     VK"
     gene            complement(23954..25900)
                     /locus_tag="PLF77_04830"
     CDS             complement(23954..25900)
                     /locus_tag="PLF77_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPC08212.1"
                     /translation="MIKNIFGYDQFPGKGCSEIIVRIFRVVFIFILILSLCACATPIG
                     VTKVEMRKSYKTINANALSADEISSDTKVLLKRYNLLDRLDNDTAAVIALLHEKALKD
                     TRRDILFALAELSFFYGERLKNATVPDQTGLDKDYFLMSSIYAYYYLYGPEGEDSPGP
                     YDRRFITASELYNRALGRGMALGTNGHWTFKERVRRLPVGEITLFPGQKSRGILLEDF
                     QSFLSVYDYDVRGLTIRNRTAGIGLPLIAIKKKTEQLPRGMAIPVTVILKVEGDISAL
                     SNNSARAFLELYSGYDEVEAVINDHKVPLETDSSTPIAYQLNDAPIWDLGIQSFLTPG
                     KKKSELLMIQPYQRGKIPLVFVHGTASSPVWWGEMWNTLSADPVLRKRFQFWFFFYNS
                     SLPFVASASDLRTILSDTIARIDPKGDDTALQQMVVVGHSQGGLLTKLTAVNTGDALW
                     RSVSDKNFEDLDTPPEMKALLRKWAFVEPLPFVKRTVYISAPFRGSFQAKNWVRGIIK
                     RIVSLPMDVLTLPYNVVAGNPNIVSNLFRQLKLPIDIGNKLPTSVDGMSPDNPILQTL
                     AALPVTPGVKTHSIIAIDGGEIPPQGNDGVVEYKSAHQEGVESEYIVRTGHSCQGHPL
                     TIEEVRRILLEHLKSVSVPPSSSP"
     gene            complement(25943..26875)
                     /locus_tag="PLF77_04835"
     CDS             complement(25943..26875)
                     /locus_tag="PLF77_04835"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HPC08213.1"
                     /translation="MTVWAALAIYYSNLPESLRPVAAIAYGLVSLLVLFVVRPRRRAF
                     MLFIGIFAVVLIWWFTIPPSNNRDWQPDVAVLPFATINGNMVTIHNIRNCDYRTETDY
                     AVRHYDRTFDLSKLQSADLFLVYWGSPYIAHTMFSFGFGDGNFACISIETRKEKGEAY
                     STIKGFFRQYEIAYVVADERDLVRLRTNYRNEDVYLYRLKAQPEIIRKVFLDYLREVN
                     RLKDTPEWYNALTDNCTTALRGHTAPYNPHAKLDWRIIVNGFGDQFIYEEKILDQSLP
                     FEKLKAVSHVNARGRAADKDPNFSQKIREGLPGI"
     gene            complement(26937..>27160)
                     /locus_tag="PLF77_04840"
     CDS             complement(26937..>27160)
                     /locus_tag="PLF77_04840"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HPC08214.1"
                     /translation="ASANPRSCCRSCIIWQIMILDSMTNKVLGVAVDKRAAGFTERFS
                     KWGSAEEAFKFWADRMVKFIDSEKTIWGQ"
BASE COUNT         7441 a         6112 c         6762 g         6745 t
ORIGIN      
        1 cttcaactgg aaacgatttc catatttgcc ggctgtggtc agaaattctt gaaaatttac
       61 gccgggcaga tttttcattt ttctgtccag ggacacaaaa aaattatcaa agtgaatcgg
      121 aataacgatt ttggcgttta gtttcagagg aacgttttcg agatatgttt gggtatgtcc
      181 cctgccgccg atacccagta aaacaatatc cgatgtgatg ccgtcataca ttccgttaat
      241 aaatccggcg ctgccgtgat gcaggattgt cccggaagca tgtgtgatca gaatggaata
      301 cgtctgtccc agtttgtaat ctcttgcgga atgccggccg gtcagcggcc ggtcgattgt
      361 cccggggtag gggactcttc ccagaaaagc cggtccgtga gcgctttcaa taaatctgag
      421 cgtgaaggct ccgattttga tgtcccggcc gatgtccgct tccttcaaac cgctttcgtc
      481 cagggggaca ccgcgcgccg cgttcagtgc gctttttgaa ccgataaaca gggcgtttgt
      541 tttaagcgca aaaaaaggag cgtcaaggca gtgatcgaag tgggagtggc tgacaagaac
      601 agcgcgaatg ttttttgttc ccggacgggt cagccatttt tcgatagcgt cccgatcagg
      661 tttcagcgaa agtcccagaa ccacgctcag cagtcccggg cggctaatgt aggggtcgat
      721 caaaataccg tcatgatcgt cctcgatcaa tacgccggca gtgcccaacc aggtaagata
      781 caaggggtcc tttttctgcg ccacgcctgg ccctccccga aaagtttggt ggagatgctc
      841 cgattatttt tctttgcccg ttttgccggg ggatgggccg ctctgaatca gtctgctccg
      901 ggcaacctgc cgttgcgcaa tcagaatgtc gcggtactgt tccacgtccg cgagcatggt
      961 tttatcgctg ttcataagaa aatccatcag cagcgatttt ttgctgataa tgaactgttc
     1021 catattttcc cgattgacgg cgtcatggta aatgcggagc atacttctgc gcaacggccg
     1081 cgatgagttg aaaatcagta tcagcaccat attgttgaca gccctggcga tcatatcctg
     1141 ttcgtccagc cagagttcaa tgaggacgtc ccggtgatcc aggttgttca gttccgaatc
     1201 catcagatcg gacagcgcct tgagatcgcg cgcggaagcc ctctgggcgg ccagtttcag
     1261 catttccgga aacatgatga tccaccactc ccagacttca tcgatgacaa acagatcggc
     1321 cgccagttcg tcttcgaggg tttctatcct ggaaaaaagc gttcgaaaga aatcaatccc
     1381 ggcgtgtttt aagaaatctt ttacataaat tccgtctccg cggcgaatgt ccagcaatcc
     1441 cattgcctcc agttgtttca aggccacgcg gagtgatgtt ctgtccacat tcagggagcc
     1501 ggacagttca cgctccgacg gaagtttttc gccgggctgc agtttatgaa aaaatatgtc
     1561 gcgcatcagc gcagtcgaaa tcttctgatg tgcctgttga ttggtcatgt ttcgtacgaa
     1621 cctcccgaat attttattgc tttctttcaa tttctatcaa actgacctga tgcattacat
     1681 gcagtcatgt cggtaagtta tcctaagatg cccatctcaa aagtgaatca cgtttgttgc
     1741 cggacgatgt ccgtgagatt caagaatggg ttctccgatg cgtcattgaa ctaaacgtat
     1801 ttattatgta tttttttctt gacaaaatca agaccaaaat atatacattg gttaaaccaa
     1861 tcagaacaat agatagtacc gcactgaaag ttgaggtcca ttgtaatgcg agatgtttat
     1921 gttatcggag ctcataccat caaattcgga aaatatattg agaagagcat taaggatctt
     1981 gccgcccaga cggttatttc gtgtctgaaa gaagctcatc tggagaaaaa agacatacag
     2041 gcgctgtggt ttgccaattc cggttgggga tacagcaagg gacaggactg catccgggcg
     2101 caagttgcgc tcaggccgat cgggattgac gccattccga tgaccaatgt cgaaaatgcc
     2161 tgcgccggag gatcgacggc tttccatcat gcctggttgg gtgttgccgg cggtctttat
     2221 gatatcacca tggccgtagg cgcggagaaa ctttacgaca acaataaaat gaaagtattc
     2281 gccggttttt taagcggtct ggatattgaa aacctttcca gcattattgc caacatttct
     2341 ttttacggca tgtcggaaca ggataaaaaa gacatgcagg cccatgtcgc aaagtatgca
     2401 gcgctggcca aaaaaaacgc cgcaataaaa aggaaaaaaa agggcgtcgg cagtcgtttc
     2461 agcgatctgc tggatatgtt caaagtctac ctgcgcctga gcgacgccgt cggttcggaa
     2521 accgtcagga agatgagaaa attattttca ggcgatcatt cgccctttat ggacgtgtac
     2581 ggatttgccg ctcgtcagca tatgaaaaag tacggttcga ccatcgaaca gcttgcgctt
     2641 attgcatcaa agaatcattt tcattcttcg ctcaacccca acgcgcaata caccttcacg
     2701 ataacgcccg aggaggttct ggcggatcgc atggtcagct ggccgctgac ccggtcgatg
     2761 tgcgcgccaa tcggcgacgg agcggcgtct gccgttttat gcgaccaagc aaccgtgaaa
     2821 aaactggggt tgtcgtcgca ggcggtcaaa atcagggcat ccgtgctcgg ttccggacgc
     2881 gagcgctctt ttgatgatac cgatatcggt gaacgtttaa gcaaaatcgc ctatgaaaaa
     2941 tcggggctgg gacccgggga tatggatttt gccgaggttc acgacgccag cgccttcggt
     3001 gagctgcatc aggcggaagc gctgggtttc tgcggtgagg gcgaaggcgg acgtttggcg
     3061 gaaagcggcg ccacccgact cggcggcaag attcccgtca atgtcagcgg cggtctggag
     3121 tcgcggggcc atcccgtcgg agcatccgga ctggcgcaga ttcacgaact ggttacccag
     3181 ctttgcggca aagccggtaa acgacaggtc gagggcgcaa aaatcgccat cgctgaaaac
     3241 ggcggaggag ccctgggaca ggaagaggcc gccatgtgca ttcacatcct ggaggggccg
     3301 tcaaaataat ttgcggcggg tatgtcccgg tatctcaaaa ttaaaaacag aaggtgaaca
     3361 cgatggatct gaataaaaaa tttcctgaaa aaagagtggt gattacgggt gcgggtagcg
     3421 gtttggggcg cgctctggca ttggaatttg ccaggagaaa ctggcggatc gccatagccg
     3481 aaatcaatga tgagcgtgcc gccgaatccg caaaaatggt ccgggagcag ggcgggattc
     3541 cgctgacgat tcactgcgat gtgaccctgc cccgcgacat ggagaacgtt ttaaccatcg
     3601 tccggagaga atggaagggt gtggatattc tgattaacaa tgccggagtg gcttctgccg
     3661 gatggtttga acggattcct ctggaaaaat gggactggat catcggcatt aatctgatga
     3721 gtatcattta cggatgccgc acgttcatcc gttatttcaa ggaatccggg acaggcggtt
     3781 acatcgtcaa cgtggcgtcg agtgcgggaa tcgcctccct gccagagatg gtcagttata
     3841 acacgacaaa agctgcggtc atctcgcttt ccgagaccct gcggatcgag ctttccaaaa
     3901 ttaacgtcgg ggtttccgtt gtgtgcccga cgtttttcaa aacaaatctg atggatcagt
     3961 tttcatcgcc cgatgaacgt cagcgtcagc tggccgatac atttttcgga aacgccaaaa
     4021 tcacgtccga gcaggttgcc cgacacaccg ttgcgtctat cgagaaaaat cgtttttatg
     4081 tgattacgca ggccgacggc agaaatatgt ggcgcatgaa gcgatggttn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ttatgtgatt acgcaggccg acggcagaaa
     4261 tatgtggcgc atgaagcgat ggttcccgga actgtatttc aagatattcg gcgtcattta
     4321 caagaaagga atttttgaca gacatctcgg gaaattattg cgggtggcca atctgttcca
     4381 gtaaatgaac gaaatcgtgc ggaaaaatca tgcaaaactt ttccggacag gtttcaggcc
     4441 gtcagcgatc gccacggcat ccgatataat tgattgtccc gcaacaccgg ccctgacggt
     4501 tatccaaaaa taaaatttat tttgagccat cttcatcttt tttagtcctt tccggaattt
     4561 tttccgtcct ctgaatgatc aaccagatga aaccgtcttc atacgccatt cccgtacatc
     4621 aaaacggatt cgatgaattt ttgtaggcgt tggcagttga aatgtgataa gagaaatcaa
     4681 taaatttaaa ttcatacctt ttatcgggca gtagtttcga tgggaattgc cggagacatt
     4741 gtcatcatcg tcatggccgc gctgctgggc ggcctgatcg ctcagatgct gaaacagcca
     4801 ttgatcctgg gatatattct tgccggggtc gtcgtggggc cttataccgg cggagtcacc
     4861 atttccgata tccacagtat cgaaaaactg gcggaaatcg gtgtcgcgct tctgctgttc
     4921 gccctgggac tggaattttc cttgagagaa ttaaggccgg tgcgcgccat tgctcttttc
     4981 ggcacaccgg tgcagattat tctgacgacg ctttacgggt ttggcatcgg tcgcatactg
     5041 gggtggtcct ggacgtcgtc cgtgtggttt ggagcgctta tttccctgtc cagtaccatg
     5101 gttctgatca aaactcttga gaaacagggg atgatgggga cgctgtccag ccgcgtgatg
     5161 atcggcgttc tgattgttca ggatctggcg gtcgttccca tgctgattat tctgccgcaa
     5221 ttgaacaatg ttcaggacgg catggcgatt ctgcttgttg ccgcggtgaa agcggccata
     5281 tttctgaccg tgatgatttt agtcggaact aaaatcatcc cccggctgat gcgatacatt
     5341 gccaactgga attcgcggga gcttttcctt atcaccacca ccaccatcgg gctgggtatc
     5401 gggtacgcaa cctatatttt cggtctttct tttgcgttcg gcgcttttgt ggccgggatg
     5461 ctgttgagcc agtcggatta cggttatcag gcgttgagcg acatcattcc tctgcgcgac
     5521 gtattcagtc tgatgttttt tacgaccatc ggcatgcttc tggatatccg gtttcttctg
     5581 gccaactggg aaataatcct gctgctggtc ggactggtga tgctgggcaa ggcggtcatt
     5641 ctggccgtct tgagtcgggt cttcggttat cataacgtta tccctctggc gatggggctg
     5701 ggcctttcgc aggtcggcga gttttctttt attctcggac gcgtcggcat aagtacgaaa
     5761 tccattaccg gcgagtttta cacgatggtg ctgaccatga cgattgtcac catgctgctg
     5821 acgcctttta tatcgggatt aacaaccccg ctgtacgcca tggcccgccg ctggatgaaa
     5881 ccgttgaaac tcgaaccgtt gaattttccc agcaaaggat taaaagacca tattgtgatt
     5941 gccggcggcg gacgggtggg catgtatgtc gccaatgttc tgcagcaaaa aaatattcct
     6001 tttgtgattc tggagtataa ttccaggcgt gttcacgaag tccggaaaag cgggttcccg
     6061 attatcttcg gggatgccgc aaaaacgatt atcctggaag cggctgatct gaaaagggcg
     6121 aagcttctgc tgatcacaac gcctgttgcg gtgatttctc tgggcattgt tgcgcatgcg
     6181 caacgcatca atccgggtat ccgcattatc gcgcgggcgg aaggcgagga acagatgaag
     6241 gccctttata caaaaggcgt gacatatgtg gtgcagccgg aattcgaagc cagtctggaa
     6301 atcgtgaacc agacgctgct gaacctcggc attccggcag agcagtttaa aacgtttacg
     6361 gaggatgccc ggcaggaact gcacaggccg ttatccgtgt aggcctcctg cggggtgtaa
     6421 aaatttattg atgaagaaag gatgattcca tgaaactgaa tctgaagacg atcataagaa
     6481 aatgtcttgg tttcgtgaat ttcagaagca tgacgaaggc gaccttatgg tggatgtggc
     6541 cgagacgcca tcgcgttttt tttatttttg aaggccgcga atttacctac ggggatgttt
     6601 accggcaggc gcagcgctat gcgcattttt tcctgtcgat cagaaaaaaa ctggtcgaca
     6661 gcggcaaact ggccagcgat cagagaatgg ccttcggggt ttatatggaa aatacgccgg
     6721 aatttctttt tgcttctttt ggagcggcct tgagcaactc ggttcttttt gccatcaata
     6781 ccggcttccg gggagacatc ctgggcaaag tgatggaaca ggcgggcatc tcttttttga
     6841 tcgtcaacga cagtacgctc aaagaggtgg agggtgttgt gaaaaatgta aacatcattg
     6901 gtccggaaaa tatttacgtc gtccgtgaag gagaaaaatc tcttcccgac ggttttcaat
     6961 ggctggacaa agcggtggaa gaagctcaac agatcgatgt gccgaaccgc taccgggtca
     7021 gagtggataa tttcagcccg gtaattgtta tttatacatc gggaacgtcg ggcatgccga
     7081 agggggtgcc gtgcacgcac atcaaaatgt tcggagccgg atttgtcgtc cagtcggcgg
     7141 ttcatctcaa caggcgcgac agagggtaca tctcgatgcc gctttttcat tccaatgcct
     7201 ggtacatcgg catacttccc cagatgattg cgggcggcag ttttgtgctc aaaagacgtt
     7261 tttccgcgag cgcttttgaa gacgacatgc tggcgcacgg ggtgacgttc ctcaactacg
     7321 tcggacagcc tctgcattac atcatcgacg cgctggagag aaaatacggc agcggtgaag
     7381 cggtggaagc cgcactggcg aaacatcccc gcaacaggtt ccgcaaggcc tacggcaacg
     7441 gggccacggt caatgaccgc attaaactta aacgttattt gggcatggac catatttatg
     7501 aaatttacgg ttccaccgag gcggtgatta ccacagccaa ctgtcccggc gatccggtgg
     7561 attccgtcgg acgcgtcgat ggttcggtgc gcatcttgga tgaagaagga aaggaatgcg
     7621 agccgggaat ggtagatgaa aaaggcgcac tgctcaatta tgacaaagcg gtgggcgaaa
     7681 tctgccgcaa ggtgggggac aacaatttga ggtttgatgg atattacggc aacgcgggag
     7741 ccaccagcca gaaatttcgc aacggctggt atcattccgg cgacctcggc catatcagaa
     7801 tcatcaaggg caagcggtat atgtttttca acggccgtac ggacgactgg atccgcaagg
     7861 acggagaaaa tttctcagcg gagaatgttc ttgatttcgc gcagaaaatt ccctgtgtgg
     7921 attgtgccat cgcctacggg gctccctgtg aagttgccga tgaaaaggtg atgataaccg
     7981 tccagctcaa ggaaggggcc gatttcaatc ccgacgaggt gcatgaatgg tttatccaac
     8041 agcagaagga ggggggaatg gatcccaagt ggatgcccga ttacatccgc gtcatcgaaa
     8101 agttttccgt aaccgatacg cagaagattc ttgtgcggcc gttcaagcgt gaaaacttca
     8161 atatagagat caatgcctca atgcgcattt attttcgccg gcgcggcgat accgcctatc
     8221 ggctgctgac gcctgatgaa ttcagaaaaa tcagggagga tttcaaaacc aacggccgcg
     8281 aagcaatcct ggcgtcgtgc tgatggcctt ggatctgttg ctttgcgggt gtcgatgaca
     8341 tgaatcaggt ttttgagaaa ggaggacaac atgttgcaga aaatcattat cgcaacgatc
     8401 atttttttat tttcattgtc gttgtgttgg gcggctgaag ggatcaaact tcccgctccc
     8461 caaatggacg ggggcaaaaa tttgatgaat gccttcaagg aaagaaaatc ggaacggtca
     8521 tacagcgcca aaaaaattcc tctcaatgtg ctttcgaaca tgctttgggc cgcctgtgga
     8581 atcaaccgtc ccgaagcggg caaacggact gctccgtccg ccatgaaccg tcagcagatt
     8641 gacgtttatg tggcgacggc cgatggactt tttctttacg aggctaaggg acatttgctc
     8701 aagcccatcc tcaaccagga tatccggccg ttgacgggga aacagtcgtt tgtcgcccag
     8761 acgccggtga ccttgattta cgtggccgat tataagaaaa tgggcaacat cagcaatgaa
     8821 gaaaaagcgt tttacgcggc gacggatacc ggctttatca gcgaaaacgt ttatctgttt
     8881 tgcgcctccg agggattggg gacggtggtc cgggggtcca tcgataaaga agccctggcc
     8941 aaaaccatga aactcggcga tcatcaaaaa attgttttgg cgcaatccgt cggttatcct
     9001 aaagaataat aattcaaaac cgcagacacc gatgcggtaa gcaaaggaga aaccaaaacg
     9061 gaagcctgtt gactgtcgtg gcctgaaaga tgcggcggga tcggtcatgg cgggttgacg
     9121 gccggggagg ttatggccgg acatgacgag acgggtttct gcggcctgtg tcgcggggtt
     9181 atagaatgat ttttatcgcc cggtgagcct tgagcaattc gtaaagggtc tgccggtggg
     9241 attcgctttc cactatcact tccacgttga gacagcgata gctgttgttc cgactgctgc
     9301 gggaaaacgt aaccagacag tttctgtcct gaataatttc atcgatcgcc attctcattt
     9361 cgtcctcatc cgtcccgata attttataaa tccaggcgca gggatattcg aggtgaagtt
     9421 tgttttctgt ttcttccatt tctttcttcg agatttttcg aattttttag gagttatccg
     9481 gaaaagctga aattagtcaa gcggtttata ccatggcccc tctgatgaat catcggaatc
     9541 atcaaccttt ttccggcaga atccggccgg caaccaagtt ttcggcattg ctttaggata
     9601 catcggggtt gaacggagac gatcgggcaa ttaacacggc aaaccaaaga ctcccggatt
     9661 tgatttcatt tttccttgac agaatccctg ctccccttta tcgttgtttt cacaaacatt
     9721 tttgtgcaaa gaaagcggat gtagggtacg cggtgaagga aagcaaaggc gtgaaaagaa
     9781 ataatcaaag cagcggaagt tttttcagca aaacggcgtc agatcatgac cgcgggggga
     9841 gaaaagatct cggaaaaggt ccccgattgt atctacgaaa gtggtttttg attctattgc
     9901 tgttcgtttt tctgcctgtt tcctcctcag ttgctgaaaa tgtcaagtgc agccgcatgc
     9961 ctctcctgat gaaaagtttt ctggccaacc attatgccat taaggatctg aacgcggaga
    10021 tcaacaatca ggtcgtcgat cagatgatca agcgcctgga cccttcaaaa acattacttt
    10081 acgattccga tgtccagaac ttaaggccgg ttctgcgaag agctttttcc agcatgcagt
    10141 cgggagactg cgccttgctc aaaccggttt acgatcttct ggtgacgcgg gcgcgcgaaa
    10201 acgaaaaaat cataagaagg attctgggat cgaattaccg gattgacgaa gatgcggaat
    10261 tgtatatcga tgtgaaaaag cgtccgtatg tcaaaaccgc ggcggaaaaa tacgatcttc
    10321 taaagaaagt tgtgcagttc cagatcgaaa acgatttact gtccggtgtg accctggcgg
    10381 aagccaaaaa acagcaggtt caccgctatg agttgcagac ggccagggtc ctggagcgtc
    10441 gtcccgaaaa gctcattgaa tatgcggcgg aggcgtttgc acaggctctg gatccgcaca
    10501 ccaattatct ttctcccgac aatctggaag acctcaaaat tcatatgcag ctgtcgctgg
    10561 agggaatcgg agcgctgctc agcagcgaca acggctttac cgttatcgag gaattgattc
    10621 cgggaggaag cgccgagaaa tcaggacttc tcaaacccaa agacaaaatc attgctgtgg
    10681 cacagaaaag agcaaaaccg gtcaacgtga tcgacatgga cctgcgcgat gtcgtaaaaa
    10741 tgattcgcgg caaaaaagga acgcaggtga cgctgatgat cctgaggcag tccgaacgca
    10801 cgtatcgttt cgatatcacg ctcacgcggg acaaggttta cattaaggat caggaagcca
    10861 aagtcgatta ccagaccaga atggtcaacg ggaagaaata cagttttgcg gtcattgatt
    10921 tgccgtcttt ctacggtgac gaaaaagaga acaaatccag ctatgaagac gtccgcgccc
    10981 ttcttatgga agcccggcag cgcggtgttg atggggttgt cctggacctt tcgcgcaacg
    11041 gcggaggttt gatcggcgaa gccgtgcaga ttgccgggct ttttcttggc agggggccgg
    11101 ttgtggcgac caaggataac agcgaaaaac tgacggtttt tgtcaacggc gcggcaacgt
    11161 ctgaaatgaa atcaagaaag aataaaatca tcagtttccc cgtggaagat cgggcgctgt
    11221 ataccggacc tctggtggtt ttgacaagcc gcctgagtgc ttcagccagt gaaattgtgg
    11281 ccggagcgct tcaggactat cgtcgtgcca tcatcgtcgg atccgatcgc accttcggca
    11341 agggatcggt gcagacactt gcgccgctgc ccagagacct cggggggatg aaagtgacga
    11401 cagggcttta ttttctgccg ggcgggaagt ctacgcaaaa gacgggtgtg ccggttgacg
    11461 tgcgacttcc cggttttttt gttctggagg atgttggaga agcggaactg gaatacccgc
    11521 tgccggcgca ggccattcag ccgttcatca aggcatcgtc aaattctgct ttactctgga
    11581 cgaccgtcga ccatccgatg gttgcaaaac tgtcgataaa atctcaggcc cggattgcca
    11641 aggatccaaa atttaaagag attattcaga aaaacaagga ggctgcggaa aagaagggcc
    11701 ttatccgcct tgttgatttg cgggaggaac ggaaaaaaga aggaggatca aataaaaagg
    11761 aaacgcccgc cgaactgaaa caaaaagttc gtgatcagta tgcgccgtat gtcaatgaaa
    11821 gtatcaatat tctttccgat atgatgaccg caaaggcagc gttgccgatg gcaaaggctc
    11881 tatagggtaa tcggttatca tcaggaagcg gacagaaaag gagggcaaaa ggcacagttt
    11941 ttggcaaacc ggtttgcgga ggatcggtgg tggcgcggaa agccggtatc tgctgcgagg
    12001 ttgccggaaa ccgcctgcca ccttttaagg agtcgcccgc ggcattgtca tcgataagaa
    12061 acgccaggca accgtaacgc gtcatgaaat tataaggccc ccagttcgag gtagggcggt
    12121 ctttcgtcca tttcagataa aatgttgtag ccgttcccag acatcatgta aatgcaggtc
    12181 cgcgtggaaa tatgcaaacg gctcttcaac gctgatatca ggggatcttt ttccatcaaa
    12241 ccgacaacca gaaaagcttt cccggtatca taatggtcat ggagaatggt ttgcagcagc
    12301 aggttaaata tcttcggatg attattttcg atggcgatgc aggcggcgac aatattccgg
    12361 agaggttctc cgggttcagg catcggcgcc gtgtgtaaga tgcccgccaa tggggcactc
    12421 aaacgtttag accaatgaag ataccatgga taatcggcaa tcagcatacg gcggaaaggg
    12481 agctgatccc agcaggccat tgcaccgcgg attctgtctt tttccacagc cacgtaaaaa
    12541 tcgtccggat gcagcccccg caaaatgccg ttgtctgcag taatgtcgtc ggccgtataa
    12601 acggggaaaa actgccttcg gcttccgtta cggttgagaa attcagcaat cgcatccgca
    12661 cccaccgcag tgccgcgcac aatccggagg cctttttgcg gccgcggtaa ccggcgcctg
    12721 aataacggaa tggcggcatt ataaagaaca cccatgtcgg tgcaatcagg aaaaccggcc
    12781 cggccgctgg tcatgatatt tctggcgccg acattgtcgt aagggatggc ggatagataa
    12841 aagcggacgc caaatccttc ctggtgccac ttcttcagga tattgtgaag gcggacgagt
    12901 atggtggtgt ttctcagcga tgaatcgatg cgcagaccgc cgatgtatcc gaaatcttcg
    12961 gggaccccgt tgagatacag cctgcgtttt gacatcgata cgacgccgac gatccggtga
    13021 ttgttttccg caacggctgt ccggtggacg tatccttcca cgtcgatggc ggaaaagaaa
    13081 ctcggttgac agtccatggt cagcgggatg cgtcccggca tgaaagtccg cttcatcagg
    13141 gcgcatatgg cttcatcgtc ctcccttcgc gcctcccgaa tcagggttgg gggaaaaaat
    13201 tcaagtgcat tttttttgtc tttgtccgtc attgtcgatt atcaatattt aaggaaccgt
    13261 gaaagcgata tccccctgtt attttttctg tatgatgtga ttatctcatg aaaaaaacta
    13321 tgctttaaat aaaattgatt aaagcctggt tattttaaac ccttccactt tcaccgcgac
    13381 cgaacggtgt aagagatcaa tggagacgac aaacatttct ttgttcaggt cgctttttac
    13441 gacgattccc tcaagcccgg caaaagggcc gccggtaatt cttgcccatt cccccttttg
    13501 cggatactga agagagaata tttccgtttt tttgttcaga aatttttgaa tcgcacgaat
    13561 ggcgtcatca ggaactgcaa cgggttctga attttctttt ttgcccagaa tacggacgac
    13621 gccgggcgtt ttcaatatat caagtctcgt ttcgtaattg ttcagatccg tatccacaaa
    13681 aagatagctg gaaaaaagag gaaccataat cttctttttt ctgtctttcc ttttgctcca
    13741 gacttccact tccggcagaa aggtggtcag atttttttgg actaggccgt cgttaacctt
    13801 gtgctcgtga cgactttttg tgtgtatgac gtaccaggac atttttcaat atacgcgatt
    13861 tttgcactga aattataaca ttgccatgtg ttttgcaacg gatttatcgt attttgattt
    13921 ttgcaggcac agccatcaca gtacggaaac atcagggccg atcaaatggg ataacggacg
    13981 ccccaggccg gtcggtaaaa aatccgtcac gcccgcatgg atatggcccg cttttccctt
    14041 gacatgcaaa acaatcgcct atagaaaaac atcatatctt tgttcctgtc aaaaatgaag
    14101 ttaccgtgga aagaacgcat aacataagcg atttggaaaa aacagaatat ctgaaaataa
    14161 atttggggaa aaatattttg ccgctgtccg cggcagaaaa caaatacatg cccggcgcgg
    14221 gtcccatcga aattatcata tcgtataaaa gaagcaccgc cgttgcccgg gggatcgaat
    14281 gtttcttaaa agccgctgac tattacaatc ttttttccag ccgtttgatt atgattggcg
    14341 aaaatacatt tgccctgcag tattgtaccg atggaattgt gatcaatatt ctgcctcccg
    14401 tccatgcgcg ttttgatgac gtaaggattg atgatttgag agaaaagatg acgcatgttc
    14461 agaccttgcc gggtcatccg cttctcgcgc ttacactgat tccggtgaac gacggacatt
    14521 ttgtcggatt cagttgctct catgcggtcg ccgacggcat ttcctgcatt ctcttccttt
    14581 atgcctggaa ctgccttatc gaagggaaac ctttcccgcg tccctctgcg cagagattgt
    14641 tcagcggaaa cccaatccct tctgatcaca ttgaaaaaga ttggatgccg cccctgtccg
    14701 cgttgagcga agagattcaa aaccgcgtca ggcggggcgc gatcaaaaca tacaaaacga
    14761 cggaatattt tgccgatgaa tttcttgctg aaatgaaaca tcaggcacag gcaaacaaca
    14821 gggaattcgt tatttccaat aatcagatta tgaccgcatg gctgttaaaa aaatatcatc
    14881 atcatattct gcctcataca gataagatcg tcatcaggac gcctgtcgat ctcagaaccg
    14941 tccatcctgg cgttgatccg atgtatatcg gttatgccgt catcaatagc ttttcggaat
    15001 ttcgcaaaaa agagattgat cacatgtcgg ttgtggagac ggcctgtcgc ttaaaagaat
    15061 ccattcatca cacaagaacg aaacgcttta ttcaggaggc aacctatttg tccaagtacg
    15121 gcatagagat tcgagaagag gttctgaaac aatttcctcc ttataatccg gacacagata
    15181 tcgtttcaac aaatatgacg catttgagcg atcttgaatc tctgttttta ggcccggatg
    15241 ccatgcgtat tgagcatatc ggcgtagcat cagtgaaaac aagttttgcc atggtgagag
    15301 aaaagaatag ccaaatgtca gcgcaaatca ccagccggta tccgctgata taaatcgtcc
    15361 aggaaagttt acatatgttg tcatccgcct cccgttacaa catccgattt gcccgccccg
    15421 aagagatccg cgatatcatt gaactgatgc gcccttataa tatgcatcac attccttcgc
    15481 cggagatgcc cccgcttgat gacaagcgat tcattgtggt tgagcaaaac ggacaattgc
    15541 tcggtgccgc cggttacacg tttgtttcag aggagatcgg gaaaacgact ctcatggccg
    15601 tacgtcccga ttgcgcccgg atggggctgg ggaaaatact gcaaaccaga cggatgcaga
    15661 ttctgcgcgg actcggctgc acaaaaatca ttacgaatac cgaccggccg gaaaccatcg
    15721 cctggtacaa acagcatttc gggtatcgcg aaataggcaa attgccgaaa attcattcct
    15781 tcagcctgga gcatgtgccg gaatggacca cattggaatc tgatctgaca accattgacg
    15841 ttccgtctcc tgttcagggg aaattgctga tgaatgctgc cctgacaggg atgaagcaca
    15901 cccgaagcga caatccttac ctgcccgtta caccggcgga aatcgctgag gcggcattgg
    15961 aagccgggaa actgggagcg gccatcgttc atcttcatgc gagggatgtc gatgaaaaac
    16021 cgacaccgga tccgtcgata tacgctgaaa ttatcagccg tatccgggaa aaaaatgccg
    16081 atctgatcat ttgcgtcacc acaagcggtc ggaactggag cgatttgcag aaaaggtccg
    16141 ccgtgctgga gttgccggat gatgtaaaac cggatatggc ttctttgtct ctggggtcga
    16201 tgaattttcc ctcgggggcc agcattaacc cgccggagat catccggtct ctggccttgc
    16261 gcatgatgga acggaacatc aaaccggaac tggaagcgtt cgaagtggga atggtcgaat
    16321 atgccaagta tctgatcagg gagggggtgt tgcgtcagcc tgtctatttg aatctgattt
    16381 taggatcgtt aggcacgatg agcgcctcac cggataatct gcgttatctt gtttcccggc
    16441 ttccggcgtt ttcgacgtgg gccgcttccg gaatcggaag atatcacagt aaagttcagg
    16501 atatggcggc cgcaatgggc ggcggtatac gaaccggact ggaagatagc agcatgcacc
    16561 tggatgcgga caagcgcata ccggcaaaaa acaaagactg gatcaagaga gcagtggaag
    16621 cggccagcct caacggccgt gaaattgcga cgccaaatga cgttcggcaa tggctggatt
    16681 tgaggaagtc gtgagccgga aaaaagtagt ttctgcctta ggaaaatgga tttcagaaaa
    16741 atggtaaaaa caacggaatt gcaacccgct gtgtcgaaaa ataaatcttt taatcgtcag
    16801 tatccgcccg tcttttttgg aaatttcgca tcggaaaatc attcagaaaa gggggaaaag
    16861 ggatgattcc taaagaagat cagaacgttt aaaatcataa aacgttgaaa tggattggtg
    16921 ccggagctcg gaatcgaacc gagatgccct tgcgagcggg ggattttgag tcccttttat
    16981 ttaactttca tttaatttca tattgcacca taaaaatacc attaaaccat tgacaatcaa
    17041 ggcttttatc attatgatat tatcacatag cgtcatataa gaacatatta tttcagcgtc
    17101 aaaagtggca acatagtggc aattttctaa ccttataggg gatatacaca tgaaacagaa
    17161 atggattgca acggcgcata aaggtcttcg gtattttgag cacgataccc ggaaacacgg
    17221 taaaagattt gaccgttatt attcgataag atttcgcgtt gataggaggc tgcatacttt
    17281 cggtgttggt tgggtaagcg acggcatacc tgaagcagtc cggcaggaag atcccggatt
    17341 agggtttgag gagtattgtt taaaattact tagacaatat aaggttaatg ctaaaacagg
    17401 aaccggacca acttcaccgc gtgaaaaacg gcttattgaa aaggaaaaag aaaagcaggt
    17461 gcaagaggaa aaggcacggt tggaaaaaga gaatatgacc tttggctatt actttgaaaa
    17521 gaaatatttt ccgacatatc aaacgggacg taaaaaaagt actagcagaa aagcccaaga
    17581 gcattttcaa aattggatta atcccgttat aggaaacacc cccctgaagg atataaaacc
    17641 ctttgctatt gaaaaaatca aaaaaaatat cctggatggc ggcaaagcgc cgcggacgtt
    17701 gcaatacatc ttagccacca taagacacgc atggaatact gcaaggataa atggcctggt
    17761 tgtgggtgat tctccaacaa agaacgttca aataccaaaa attgataata agcgcgtccg
    17821 attcttgaca cataaagaag cagaaaacct tctaaaatca ttaaaagaag aggacgtatt
    17881 gacctattac atttcgttac tgagccttca aacagggtta cgttttggtg aaatagccaa
    17941 attaaaatgg ggtcatattg accttgataa aggaattatt gaagttgttg atcctaaagg
    18001 taacccgaca cgtattgtct atatgacggg caaaatcaaa gctatgtttg acaaaatgga
    18061 acgtcggaag ccagacgatt atgtattttt taaagatggt gcacaattaa aagacacacc
    18121 aaaagtattt tttaaagttg ttgatgaatt aggattaaat gacggtgttt ctgattctcg
    18181 gcgaaaagtt gtgttccatt cgctgcggca cacctttgct tcttggcacg tcatggccgg
    18241 tactgacatc tataccgtca aaaaactaat gggccattca aatattgcca tgacggagag
    18301 atatagccac ctggcaccgg aggccttgca gaatgcgacg cggacgttgg aaaaggctat
    18361 aaataaagca gggcataaga agatcgggca agtggtaaaa tttcaaaaat aagcttagca
    18421 ataaaaaaac ttgcgccaaa gttaaataaa tgttaatata ttaatcacgt caaggtatcc
    18481 cgccatcgat ctggccgcgc gaaagcccag ctggaattga ttatcaatag atggccgcat
    18541 agtttgataa gctgaccgcg ctttttaagc ctcgttggat taaaccagcg gggctttttt
    18601 tatgttttgc ggtataaaga acaaagactt tagtaacctc aatcttcatc tgggcaaaca
    18661 aaccgagcag accacaggcc gcaagtgtca ttcttcggcc aaacaagcac taaagaaaag
    18721 tcgcgcttta aaggaattat tacgcctgat ttatccgcag gggaatctca ggctttttaa
    18781 tttttactaa aggagagaat tatggaatca gcatttcaat gcttagtaga caaatggccg
    18841 tcgacatttg tagcacggag cgaagtcaaa aacttttccg gaggtgcgct tacggaaggc
    18901 tatcttgcca atctcgacag tcaagggaag ggtccgaaag ggcgagtccg ttgcggtaga
    18961 aagattctct atccagtcaa tgaattggtt gagtggctgg aggcacgaag cgaagttctt
    19021 acggaacgaa agaagtaaga gtcgcaccat gcaaaacaat ggagggtggc caatgaatca
    19081 caacgaaatt attgaacgga aaatatttct caaacgtcaa ttgattgtga tcatcaaaaa
    19141 agaaattaag aaacttgaag gtagcttaaa gcatataggt gaagaagatg ataggcgttc
    19201 aatggatcaa aataacaacg tcaatgtttg atgatgaaaa aataaagttc atcgacagtc
    19261 taccggagcg cgacacaatc ttttatattt ggattcgctt gctcgtattg gccggaaagt
    19321 gcaacgctgg aggatatatt ttccttactg agaacatccc ctatactgag gaaagtcttt
    19381 ccagcgtcat aaaccggccc ttaaatacag tcaaattggc catgtcaact ttcctgcaat
    19441 taggcatgat tgaaactgat tcaaagggca tatacctgct taattttaac aaatatcaaa
    19501 acattgaagg tcttgaaagg attagagaac agacaaagaa acgagttgcg caatatagag
    19561 aaagaaaaaa actcttagaa tatgatggcg gtgtaacgtt acctgttacg ctatgtaacg
    19621 gaacagaaaa agaaagagaa atagataaaa tatatattca gaatgccctg acagttctta
    19681 cctatctgaa tgagaaaacc ggcaaacggt atcgaataaa aaaatatatc atggcacgtt
    19741 taagggatgg ggccacaatc gaagagtgcc agagcgttat tgacgcaaaa ataaaagatc
    19801 agttctttat tgataacccg aaatatttaa accctgaaac attattccgg ccatccaatt
    19861 ttgaccgtta taaaaacgaa acgattcctt tcaaaactga aagcccaaaa acaaaaatta
    19921 attcctgttc gcgctgtggt tcacaagtgc cgccgcagga catgaccgcg aacgggtgca
    19981 tttattgtga aggcaaggag gccagcgcat gaccgtgaat aaagttcttc cgaataatgc
    20041 agaagcggag gccgctgtcc tgggcgcggt actcatggaa aacagagcat tgaatgagtg
    20101 cctttcgata atcgggccgg atgattttta cagtgatcgc aacaggcttg ttttcttggc
    20161 catgtgcgaa ttatccgaca aaggtaagcc gattgattca atcatactgt caaaccatct
    20221 caaagcacgc ggccagctcg acaggatcgg cgggcagctt tatatatctg atctgctgga
    20281 cagcaccata tcaagcgcga aagttgcgca ctatgcccgc ataatcaagg ataattctgt
    20341 tttacgctcc atcatcaaag ctgccggtga catggctgat catgcctttt ctataaacgg
    20401 tgaagggcct ggcattgttc ttgacgaagc catgcgctca ttgatgaata tttcaattga
    20461 ccgaaagcgt catgatgtca gggattcaaa agatataagc aagacaacct tcaaggccat
    20521 tgaggaacgt cacgaaaaca ctaatttcat aagcggctta tccaccggct tcaatgagct
    20581 tgattgctgg acttccggat tgcaggaatc cgaattgata ataattgccg gacgaccagg
    20641 catgggtaag acggccttcg gcgtgaatat tgtagagaat gttgttttat ccggcgtgcc
    20701 cgccgtcgtc ttttccctgg aaatgtccag cgaatccctt atgacgcgca tattttcatc
    20761 attggcgaat attgattcaa ggcggctcag atgtgggcga atttctgatt ctgattggcc
    20821 gcgtcttgtg gaaatcgtga atgatataag tgcggcgccc ttgttcattg atgattcgtc
    20881 agtcttaaca cccatgcagt taaaggcgaa agcccgccgt ttaaagatcg aaaaaggcat
    20941 tggcctgatc gtgattgatt atttgcagct catgaccggc accggcaaga ccgaatccag
    21001 ggagcgcgaa atagcggaga taagccggtc actaaaggca ctggctaaag agttacatat
    21061 ccccatcatc gcactatcac agcttaaccg cgcagtcgaa aacagaagca aaaaagagcc
    21121 gagcctcgcc gatctgaggg aatccggcgc aatagagcaa gacgcggatg tcatcatatt
    21181 tctttatcgt gatgaggttt acaacaagtc agaggttaat cctgagcgcg gccttgctga
    21241 agtcagcata gcaaaacagc gtaacgggcc aacgggtact tttaaaatgc ggtttagtgc
    21301 ggagcttaca aaatttcacg attttggaag acaatgaaat ttttaaagat agagacttat
    21361 aaactatcgt taaaaagagc gagaggtata tcaaacttat agattgtaag cgtaccagaa
    21421 gtatatcaaa aatgaacgat atgaaaacac aggaacaaag aacagagttc ataaaattga
    21481 gagcagaagg caaaagttac acttttattg ctggtgccct gaatatcagt aaaagtacct
    21541 gtaccggctg ggagagggag tttaaggacc agataagcca gcttaaaagg gaagagctca
    21601 atgaactata tgattcttat tatatgacca gagaagccag aattaagcac cttggagaca
    21661 cactggtaaa cattaatact gttttagaca gtaaagacct ctcaacaatg tccccggaaa
    21721 aattactgga tttcaagctg aaatatacag aggccttgaa agctgaatac atggatactg
    21781 atccaccgca gcggttgggt gcgaacttta aagcacaaga tatattaaag gccttgggca
    21841 acctacttga ccgtattaga gctggcgaag tcagcatgga acaggccggt cgggagagct
    21901 tggttatcag taatctctta aaagcttatg acactgtaga actacagacc aaacttaaag
    21961 aattagaggc gattatcgga ggacgagaat aatgaaaacc gacataaaca atttactaaa
    22021 gaagggcaag ctcacggggc aggaagtagg acaattgatg atccaagatc ttgtggcaac
    22081 ttataaaaac tatcttgaag gtaaaggcga tgaaggtttt ttaacccagg cagaaactaa
    22141 tactcttgtg aacggtctta ccaagtcaca agacataaag aggtatcgag agttcagagg
    22201 tgtacatgaa tacattgtga atgcggcgag agcctttgtt attcacgaag aaataatgga
    22261 agaacactta tggaaaataa attacatatt caatgaaatg aggcacgctg aagaagagaa
    22321 ttttcaggct atacgtcaac ctcgtattat gacgcaaaaa caatatgacg aactgaaaag
    22381 ggaagacttt gaagaaaaaa tgacatggac ctatagtttt gagatgcttt tatttgatgc
    22441 attggaatat tacatcaacc ttcataacga aggagaaaaa acaccctttg ataagcattt
    22501 cgccgctgcc aagaaacaac ccataaagaa tcctcgcata aaggctgatt attatgaaag
    22561 cgaatcagaa gataaaactg cggcggatac cgcaacaatg ttcgatgttc ttgaatacgc
    22621 taaaggtttt tattcgagtt tagagactga cagccagaaa actctgtttg agtttacaga
    22681 agatttccca gggctctaca aagacctctg gagcaagctc acagctatga aagggcttaa
    22741 tttcttgaaa gatattccga agaagaagta ttttgatgat gctctgatca gcgttaggga
    22801 actttatgag aataatattc tgaattttag agattttgtt ggaacatttc accctgatgg
    22861 ttgcggtgga ggcgtagctg ttttgcaatc ccattgtttt tatcccagaa gaaatataga
    22921 cgagcacggt tattatagag caccggagcc atattggaga aaacacttta tggcggaggc
    22981 aatattatca gaacccttga gaaggacaat taccagatgg atagctttat tcagtgagca
    23041 tttaaaagaa tgtttcgtca ttagaacagc ttttctgctg attggagaat ttacaggtgt
    23101 gcctcaaata tcaaccctta tagatggcat agatggagag atagatagac tcactaaacg
    23161 aggagagtta gttaatcaaa ttattgaaaa tgcgcatgac ttgattctga gtcgctacgg
    23221 agctttatcc aatgaacgtt ctgtggatga actgagatca gaatttaaaa atacgtttcc
    23281 cctgatagac atgaaatatt taatgccctc ggaagatgct atagaagaag cgagacagac
    23341 actagacttc acgatgattc aaaatgatgc tgaacagctt tacgctattc tgaaaaatga
    23401 agaagttgaa aatacagaaa gtacgttagc ttgttgagtt tacctacaaa gaaatatttt
    23461 tgaaaattcc tgatgttatt gattactaat tgaaagaaat tatatgggag gttggtatca
    23521 aatgggaccc tgtggcataa tattatttga cacgcaagag aacgtttctt atactgctga
    23581 caactaaaag caagttctta tcaattagtt tggccccgaa tacggcggtt tcatcaatat
    23641 cgatatttaa gtcatactcg tcacaatccc aatcatcaca atactcatac gttaaatcgg
    23701 gagctatcgt atagcctccc cagatgccaa aatatcctcc ggtctgataa gggccgctat
    23761 aatattctcc actttgagcc gacgctgact gcaatggata agcagcaaat acgacagcta
    23821 ctgccaagat aaaaatataa ccggcgatct ttttcatatc tcttctcctt tccttatttt
    23881 gaacctatta taaatcttct taaagaattt tccaagcttc ggtttaaccc ttcattgccg
    23941 atgtgttccc tgcctacggt gatgatgaag gcggcactga aacggacttg agatgctcca
    24001 ggagtattct tcttacttcc tctattgtca acggatgccc ctggcaggaa tggccggtgc
    24061 gcacaatata ttcggactcc acaccttcct ggtgagcgct tttgtactcg acaacaccat
    24121 cattcccttg cggcggtatt tcgcctccat cgatagcaat gatggagtgg gtcttgacac
    24181 cgggagtaac cggcagcgct gccagtgtct gcagaatcgg attatcgggc gacatgccat
    24241 ccacactggt ggggagtttg ttgccgatat cgatgggcaa ttttaactga cgaaacaaat
    24301 tactgacaat attaggattc ccggccacaa cattataggg aagcgtcagg acgtccatgg
    24361 gcagggacac aattcgctta atgatgccac ggacccagtt cttggcctga aaactgccgc
    24421 gaaacggcgc ggaaatgtac accgtccgct ttacaaaagg aagaggttca acaaaggccc
    24481 acttgcgcag cagcgctttc atttccggtg gcgtgtccag gtcttcgaag tttttatcgc
    24541 tgaccgacct ccagagcgca tctccggtgt taaccgcagt caattttgtc agcagccctc
    24601 cctggctgtg gccgactacc accatctgct gcagcgccgt gtcatctcct ttgggatcaa
    24661 tcctggcgat cgtatcggac aggatcgttc gcaaatcact ggccgaggct acaaaaggca
    24721 gactgctgtt gtagaagaaa aaccagaact gaaagcgttt acgcagcacc ggatcggcgc
    24781 tgagcgtatt ccacatctcc ccccaccaga caggactgct ggctgtgcca tgaacaaaca
    24841 cgagcggtat ctttcccctc tggtagggtt gaatcatcaa cagctccgat ttctttttcc
    24901 cgggggttaa aaacgactga attcccagat cccagattgg agcatcattg agctggtaag
    24961 cgatcggtgt tgaggaatca gtttccaaag gaaccttatg gtcattgatc acagcttcaa
    25021 cttcatcata tcccgaatag agctccaaaa aagcgcgggc ggagttgttg ctcagggcgc
    25081 tgatatctcc ttcaaccttc aggataactg taaccggtat ggccataccg cgaggcagct
    25141 gctccgtttt tttcttgatg gctatgaggg gcaaaccgat gcctgcggtc cggttgcgta
    25201 ttgtcagacc gcgcacgtca taatcataga ccgacaggaa ggactgaaaa tcttccagca
    25261 aaataccgcg agatttttga ccgggaaaga gcgtgatttc accgacagga agacgtcgga
    25321 cacgttcctt aaatgtccag tggccatttg tcccgagcgc catcccccga cccaaagcac
    25381 ggttataaag ctcgctggcc gttatgaatc tgcgatcata aggaccgggg gaatcttcgc
    25441 cttccgggcc atagagataa taataggcat agatggacga catcaggaag taatctttat
    25501 ctaatcctgt ttgatcagga accgtcgcgt tcttcaatcg ttctccataa aagaagctca
    25561 attccgccag agcaaaaaga atatcccgcc tcgtatcctt cagagccttc tcgtggagca
    25621 gagcaatcac cgcagccgtg tcgttatcca gacgatccag aaggttgtaa cgcttcagca
    25681 gaacctttgt gtcagagctg atctcatccg cagagagggc attggcattg atcgtcttgt
    25741 acgatttcct catttccacc ttcgtcacgc caatcggtgt ggcgcaggcg cagagtgaca
    25801 aaatcagaat gaaaataaac accactcgga atatgcgtac aataatttca ctgcagcctt
    25861 ttcccggaaa ctgatcatat ccaaaaatat tttttatcat caccggactc ctttctccga
    25921 ttctcctgga acaattctga atttatatcc cgggtaatcc ctcccggatc ttttgcgaga
    25981 aattgggatc cttatccgcg gcccggccgc gggcattaac atgacttacg gccttgagtt
    26041 tttcaaaagg cagactctga tccaggatct tttcctcata gataaactga tcaccaaaac
    26101 cgttcacgat gatgcgccag tcgagcttcg cgtggggatt atagggagcc gtgtgtcccc
    26161 tgagcgcggt cgtgcagtta tcggttaggg cgttgtacca ttcgggcgta tccttcaacc
    26221 tgttcacctc ccgcagatag tcgagaaaaa ctttccgtat gatctccggc tgtgccttca
    26281 ggcgataaag atagacgtct tcattgcggt agttggtccg caagcggacc agatcgcgtt
    26341 cgtcggcgac gacataagca atctcgtact gtctgaagaa tcctttgatt gtggaatagg
    26401 cttctccttt ctctttacgg gtctcaatgg aaatgcacgc gaaatttccg tcgccgaagc
    26461 caaagctgaa catcgtgtgg gcgatatacg gagaccccca atacaccaga aaaagatcgg
    26521 cgctctgcag ttttgacagg tcaaaggttc ggtcgtaatg gcgaaccgca tagtctgttt
    26581 ccgttcgata atcacagttg cggatgttat ggatcgtaac catgtttccg ttgatcgttg
    26641 cgaacggcaa taccgccaca tcaggttgcc agtctctgtt attgctgggt ggaatggtaa
    26701 accaccagat taaaacaacg gcaaagattc cgataaagag catgaaagct ctccgtcggg
    26761 gccggacgac aaagagaaca agaagggaaa caaggccata cgcaatagcc gcgaccggac
    26821 gaagtgactc cggcagattc gagtaataaa tggccagcgc tgcccacaca gtcattaagg
    26881 agaatatcag gacaagcaat atccacccgc atattttcaa gatctttata aacaaattat
    26941 tgcccccaaa tagtcttttc gctgtcgatg aacttcacca ttctgtcagc ccagaactta
    27001 aaggcctcct cagccgatcc ccattttgaa aatctctccg taaatcctgc cgctctttta
    27061 tcaacagcca cgccgagtac tttattggtc atgctgtcca gaatcattat ctgccagatt
    27121 atgcatgacc tgcagcatga gcgtggattt gccgatgccc
//
LOCUS       DAOOLB010000024        26351 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_1692_length_26351_cov_43.352829, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000024 DAOOLB010000000
VERSION     DAOOLB010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 26351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26351
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1692_length_26351_cov_43.352829"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..1556)
                     /gene="mnmE"
                     /locus_tag="PLF77_04845"
     CDS             complement(177..1556)
                     /gene="mnmE"
                     /locus_tag="PLF77_04845"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPC08215.1"
                     /translation="MLRSDTIVAAATPFGMSGIAVIRVSGPEALAVARRIFRPSNRPC
                     DWKSHHLYHGDIVCADGETVLDEILIAFMRKPRSFTGEDVVEISCHGNPVIIQSILDQ
                     LQKLGCRLAGPGEFSQRAFLNNRFDLSQAEALASMIGAQSEKAYAVGLAQLKGSLSKE
                     IETIRGLVIEALALMEASIDFAEDTAEAELVQIPPQINQAMERMQRLLTSYRFARAYS
                     EGVHVVITGKPNVGKSSLLNALAGKKKAIVTDIPGTTRDLIADTIHIGGIPVHLTDTA
                     GIREPQNVIEQEGIALVWKKLAEADIVIIMLDVSKPLTEEDKITIDKNTSEKIIVAVN
                     KIDLPRSWETKSIANLFPPHTKFLEISAKSGSGLEELKRTILDLSVDGETESTSGAIL
                     TNLRHKHAVEQSLKNIQNAKESVTAGLSAEFIAFDLREALDHLDEITGKKINDEILDR
                     IFSSFCIGK"
     gene            complement(1575..2459)
                     /gene="jag"
                     /locus_tag="PLF77_04850"
     CDS             complement(1575..2459)
                     /gene="jag"
                     /locus_tag="PLF77_04850"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HPC08216.1"
                     /translation="MSSEFIEIEEKTIDEAIEKACQTFGVPREKLNIEIISGESGGFL
                     GLFSKKAKIRASLLALDIDFSFSDKPKEKTDKPREKNEPRSKADHYREKSEAKVLPKR
                     KPEKEVKEISEEKSPVKKEREAFIPNSPLSLKAQELLEGILQRISPEYKANVQETQEK
                     IILGIEGDESGLLIGKRGQNLDAFQYIINKAINKIDSEHKMILIDSGEYRKRREDFLL
                     DLAEKIKKKVKKTKKPVSIANMNAHDRRIIHLVMQEDESLVTQSRGEGKYRKIIILPA
                     KNGSSRRTKEKKKDINQS"
     gene            complement(2493..>3997)
                     /gene="yidC"
                     /locus_tag="PLF77_04855"
     CDS             complement(2493..>3997)
                     /gene="yidC"
                     /locus_tag="PLF77_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPC08217.1"
                     /translation="NVLPAQEESSRDIKVETANYTAVFSTKGAALKSFQLKGYQKECT
                     KCVGDIWPAIKNFVTGKKQPAGEKNNDLIEVVSLQPDMPYPLTLTFPESSGEIAADSV
                     YKTDTAELNMVKNSKKQRLVFSRVFNDVRVEKIYIFNPDNFSITLEVNISNLTDVPIT
                     KIPYLNWYDYVDPKEKVDGYSKEGPVAYVSKSIERWKSADVSKEQTIGPDVSWGGVER
                     KYFMAVIIPQDPSLTSFNMSRDDHVIAVNLKGAKNIIPPEQSGSFKYNLFIGPKDYAM
                     LKKLGLSLEEAIEFDSVIPGLKWLSIGLLLFIKFIHQFVGNYGVSIIILTILIKVLFW
                     PLGNISYKSMKEMQKIQPQITALREKYKNDQAKLGQETMALYKTHKVNPFGGCLPILI
                     QIPVFIGLYNTLLYAIELRHSPFFFWIQDLSAKDPYYITPIVMGATQFIQQKMTPTMG
                     DPMQAKIMLAMPIIFTFIFLNFPSGLVIYWLVNNIISIGQQIYINKKLTD"
     assembly_gap    3998..4097
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4098..4363)
                     /locus_tag="PLF77_04860"
     CDS             complement(<4098..4363)
                     /locus_tag="PLF77_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08218.1"
                     /translation="MDKRTILAVVLSLAVLFAYQIFFAKPPVPPETVPVRESHQTAQK
                     PTPIKENSVVKQQAVFPDASTPKQGVKNVLPAQEESSRDIKVETA"
     gene            complement(4382..4609)
                     /gene="yidD"
                     /locus_tag="PLF77_04865"
     CDS             complement(4382..4609)
                     /gene="yidD"
                     /locus_tag="PLF77_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002474682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPC08219.1"
                     /translation="MLRKIFINFFVSFIHLYQACLSPFFFAPCCRFYPSCSQYAIEAI
                     KLYGPAKGSWMGIKRILRCHPLNPGGYDPVK"
     gene            complement(5008..5145)
                     /gene="rpmH"
                     /locus_tag="PLF77_04870"
     CDS             complement(5008..5145)
                     /gene="rpmH"
                     /locus_tag="PLF77_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPC08220.1"
                     /translation="MKKTLTNKTNTKRKKTHGFLVRMASKAGREVLNRRRAKGRKRIA
                     V"
     gene            complement(5304..5972)
                     /locus_tag="PLF77_04875"
     CDS             complement(5304..5972)
                     /locus_tag="PLF77_04875"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC08221.1"
                     /translation="MKSSLQKLSKPSGSRSSSNEGNVRERILKAARDVFTVYPFKEAG
                     TRMIAKQAGVEHPMIHYYFGSKENLFYAMAEDLYHETLSVQEAWLDGMENMSLKEGFE
                     LFLDRMLDYTMQKPDALRIAALNVVNIGNLEEIPGYRFIIMSMASMRRIIEEKLSLKG
                     SNREIEMFIYCFYSLMISLIGARSCQAQILNMNPLEEKYRTWVKDASMTLFYPWFKKI
                     LVGR"
     gene            6262..6831
                     /locus_tag="PLF77_04880"
     CDS             6262..6831
                     /locus_tag="PLF77_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPC08222.1"
                     /translation="MAKFKAPSGVSVNDFFEKLVPEQFKEVIAGANLSSLTGKEVNLQ
                     FNVNDQQYCLKIKDGTNLDVVKGSVDKPLLTLSLSEQVFLDAVTGKAEGLIDRFTDPV
                     EIADPMRLKNLMDTKGTLNLKLKQDGNEIPVTMIFNGEEAPKVDISLDLQDWIAMQNK
                     QTTGQNLFMNGKLKFAGDMTLLMKLQTLI"
     gene            7024..8133
                     /locus_tag="PLF77_04885"
     CDS             7024..8133
                     /locus_tag="PLF77_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPC08223.1"
                     /translation="MEDRDFGFGMSEEQSSMKDTVAKLVKSMVSGNPAHDMDEARKLP
                     REIIDKVWELGATVSTVPEEYGGYGMEYSPIMNAIFVEELAAGDMALAIASTLPATFI
                     YTILQMGTDEQKKKYLPLYCTPTYQACTVAINEPLFRFDAVSLETKAVKNGSKYIING
                     KKCFVPMAKDSGHMLVAADIDGKMDLFIVEGNNPGLKIGEREKNLGMYALESFPVVLE
                     NCEVPDDDRVGGDNGCDYSRFLQKTRIAMSAIGTGVSRASYEYAREYAKERIQFGEPI
                     ASRQAIAFMIAEMAYEVDAMRLMTWKAASALEAGRDAKKEAYLAKLYAGEMTMKLTDF
                     GVQILGGHGYIRENPVERYYRNGRGIAILEGMATV"
     gene            8145..9341
                     /locus_tag="PLF77_04890"
     CDS             8145..9341
                     /locus_tag="PLF77_04890"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPC08224.1"
                     /translation="MINLEMTQKMMDVKSGMNNMASSMLRPVSRHYDEHEHEYPKELD
                     VFRSIKIMSRPKKKKEGGETKPDKPGAKKLGANLSMVIVIEEMCWGDLGLLLSLPNAG
                     LGNAAIAAVATDEQMERFGEKWASMAITEPGSGSDSGSITARATLEGDEWVLNGEKIF
                     VTCAERAEVAVIWATVDRSAGKAGIKSFVVEKGRPGFKLEKLEHKLGVRASDTGTYVL
                     DDCRIPKDNILGSAEVKQSTEGFKGAMKTFDNTRPLVASMALGVTRASLDFTQEKMEA
                     SGITLDYEKNPNNLSAMEKEFYLMEAHLDAMRLLTWRAAWMMDNGEFNNLEASMSKAK
                     AGRYGTFICQKCCELLGPLGYSCENLAEKWMRDVKITDIFEGTGQIQHLIVARNVLGY
                     SSKQLK"
     gene            9540..10004
                     /locus_tag="PLF77_04895"
     CDS             9540..10004
                     /locus_tag="PLF77_04895"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HPC08225.1"
                     /translation="MADQSKIGMEFPASTFIIEKDKIIEFAIAVSQKEMKNQIHPVYH
                     DEKAAKAAGYEGIPIPPTFLTSSFFWTGDGLTGMVTALGINLKRLLHREEEYEYFCPI
                     HAGDIITRKMKVADIIKKGKGARTLDMTILEVEYINQRNELVAKSRTTLTEI"
     gene            10048..10485
                     /locus_tag="PLF77_04900"
     CDS             10048..10485
                     /locus_tag="PLF77_04900"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HPC08226.1"
                     /translation="MPEYISLPITRTHLVRYAGASGDFNPLHHDQTFANTIGLQNVIA
                     HGMFIMAIAGEAITSWIGNKNIKKFHSRFLAKTEPVDWDNFENTKDRATIVVKGKVIR
                     LFVENGEKLFECKIVAENLLGSKKLSGSFIAVVTGEPPRDVNE"
     gene            10494..11816
                     /locus_tag="PLF77_04905"
     CDS             10494..11816
                     /locus_tag="PLF77_04905"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC08227.1"
                     /translation="MKKRILVVDDDAVACEFLEETLKRDGYDVTLCSSAWEALRQNIA
                     SYNLVMSDIRMSGMDGLQLLEELQKQSPDLPVILMTAYGSLETTMAAVNKGAWDYISK
                     PFSPEDCRTLVRKVLELRKLRHKGMKLQPAKVEESKFIGSSAMMVDFYKQIARIADSS
                     ASVLIEGESGTGKELTARSLHNMSSRREKPFIIVHCGAIPDNLLESELFGYNKGAYTG
                     ASHQHTGLLETAEGGTVFLDEITEMSTALQGKFLRFMQNGELRKIGDNAVRNVEVRVV
                     AAANKKIDDEVKKENFRLDLLYRFIVRLRIPPLREHKEDIPLLTEALLKKLGDTNVSV
                     SPDAMEMFMAYDWPGNVRELENVLQQIMLLSPFDEILPENLPERFHSSTDRKDILNGP
                     GLSPLEEAEKEKILQTLKETSWNQSQAAKRLDIDRKTLRTKIRRYSLS"
     gene            complement(11865..13415)
                     /locus_tag="PLF77_04910"
     CDS             complement(11865..13415)
                     /locus_tag="PLF77_04910"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08228.1"
                     /translation="MAKTILKKITAFNNRLTVRSKLLVLIVSGLALTMGVWGIIQLAA
                     LERILVEQQAKRLKDVAETVSTFYQDFPTKKGLKALESALHDHLQADGRLARIDIFDI
                     GQKHFDYVAGASRDPYQWADNIVMSVMKKNKLHYMKIMTENGPALGLLYPVAGNTKSV
                     RIVIGIISFSRANEEIVHRAGRLLIISSAGLLIFILVVLGLSYRWIIGKPLKLIIHAI
                     DEFQEGQYDNRIPIVHRDEWGQISRHFNMMADEIQDVMERNQELTKHLEKRVHEETIK
                     VFQLQKQVTDLKQLTTLGQLTANLAHDLGTPLHSIAGLAKLLLEKGGLPENVEHKLNL
                     IVQQTLRLDAVIQNVRKAIRLPDPSFEALPVEQIFHEMLSLMEPFLQKNNVESVVTIT
                     NPVSPLYVDRYRIQTALMNVMQNALEAMPEGGTITMSAHEVPCENFVAITVSDTGYGM
                     TPDVVENVCEPFYSTKTSKGLRGLGMAIVRDVMKIHRGQMEIKSSRGQGTMVILCFPK
                     LKDADHFH"
     gene            complement(13768..14055)
                     /locus_tag="PLF77_04915"
     CDS             complement(13768..14055)
                     /locus_tag="PLF77_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08229.1"
                     /translation="MKKVVLLVVALIFSFSMTVAAVEKAEVPDTSEPVKVADIEKDDI
                     KKGDVKKDKTKKSKKKNVKKSKDKKSKKSSKKDEKIVEDAESEVPAAPAAK"
     gene            14430..15767
                     /locus_tag="PLF77_04920"
     CDS             14430..15767
                     /locus_tag="PLF77_04920"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPC08230.1"
                     /translation="MQKEKNENDIIVIGAGAAGMMAAGRAGELGAKVLLLEKTDDCGK
                     KILVSGKTRCNLTNAAELNHFIAMYGDNGRFLFSAFHHFFRTELLDFFNHHGLATKVE
                     RGGRIFPVSDDAHDVVRIFKKYLSENNVTLKVNTKVTGITMKDDSVYGVKTKDQDYRC
                     KAVVLATGGATWPSTGSTGDGYKIVAAWGHNIVKPKPALVPLIVREQELAQSMQGVSL
                     RNVRATAFQGEADSIDSRMTPDCIYGRGEKKSPRHPVIESRFGEMLFTHFGLGGPIIL
                     LMSLSVVEALEKGLVSVLIDLKPALTREQLHKRLQRDLDNFSKRKISGILKEYLPAKM
                     IEPFIGLTGINNEKLAHQINAAERGKIVEILKALRFNIKASLPLEKAIVTAGGVSLNE
                     IDPRTMASKLISGLFFCGEVMDIDADTGGYNLQAAFSTGYLAGEKAVEYIKAS"
     gene            complement(15780..16130)
                     /locus_tag="PLF77_04925"
     CDS             complement(15780..16130)
                     /locus_tag="PLF77_04925"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPC08231.1"
                     /translation="MKSINYKTVAPTHFDKDSARGVAGRVVTGKADGASNFCMRVFEI
                     APGGFTPKHSHPWEHEIFIHAGSGEVYGNGQWNPIDPESVIFMPAGEEHQIRNTGKET
                     IVFVCLVPSVAPEL"
     gene            complement(16145..17503)
                     /gene="lpdA"
                     /locus_tag="PLF77_04930"
     CDS             complement(16145..17503)
                     /gene="lpdA"
                     /locus_tag="PLF77_04930"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPC08232.1"
                     /translation="MSEKYDLIVIGGGPGGLTAAEMAAKKKKRVLIIEKEGWGGTCTH
                     RGCIPTKALLSCSKYYADLKKLKRMGINIADSSVDFPAVKKHQQQIVKVSALGAQKML
                     NDAGAEIKQGVGEIISPSEVRFTDSSGKSENLSTQNILMAWGSIPQVLSGINLSERIL
                     TSDGVLNLNALPSSIIIVGGSVIGAEFATFFAELGTKVTMVEILDTIIPLEDQECADL
                     LKQELTRMGVVIHTSTKLESLKDTGSSVAMVAQKDGKHLEISADYALLCTGRKPLLFT
                     AELDKCGIEYTQAGIKVDKKMMTSVAGIYAVGDVTGGMMLAHRAMQQAKFAVERIFGG
                     DDFAYNEDCIPSVVYSHPQIARVGYTQKRAGEEGLSVEVVKSPYSANIIARAELTGQG
                     FVKAVFHQNKLIGATIVGEDAAELIVPLALIVSNQMTKKQLKGWVLPHPTLSEVFALL
                     TG"
     gene            complement(17561..18424)
                     /gene="lipA"
                     /locus_tag="PLF77_04935"
     CDS             complement(17561..18424)
                     /gene="lipA"
                     /locus_tag="PLF77_04935"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HPC08233.1"
                     /translation="MNPLNEKSSIIRKPDWLKVRPPFSNECRKIRSVLNDLQLHTVCQ
                     EAACPNMAECFASGTATFLIMGNICTRNCLYCNVEHGKPAAIDENEINHLIDAVQKMK
                     LEYVVITSVTRDDLRDGGAHVFADCIRRLRKTVEHCKIEVLIPDFQGNPSALTKVIDV
                     QPDVINHNMEVVKPMFTRLRPQGNYDVSLKLLKNIGPSSVVSKSGFMIGFGENRDDIL
                     RLIDDLAAVSCKRLTIGQYQQPTLKHWPVAKYYHPDEFAEFKEIAYQKGFQHVESGPL
                     VRSSYHAAKAG"
     gene            complement(18499..19941)
                     /gene="gcvPB"
                     /locus_tag="PLF77_04940"
     CDS             complement(18499..19941)
                     /gene="gcvPB"
                     /locus_tag="PLF77_04940"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003346.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HPC08234.1"
                     /translation="MELIFEISKPGRAAAGIPASDVPSINIEQAIGRQYLRDDLDLPE
                     VAEIDLVRHYTNLSRRNFGVDLGFYPLGSCTMKYNPKINENAAALPGFTGLHPYADVK
                     SAQGNLRLLYDMQKYLCAIFGMAEFTLQPAAGAHGELTGVMMIKKYFEKKGQKRRIIL
                     IPDAAHGTNPASSALCGFEIRELKSNGEGGVDIEHLESLMTEDVAALMLTNPNTLGLF
                     ERNIEKVAAIVHGKGGLLYGDGANANAFLGKVRPGDLGFDVIQLNLHKTFSTPHGGGG
                     PGSGPVGVSNALLDFLPVPRIIRKDDKFKWSEKFPDSIGRVRAFYGNFNVIVKAYTYI
                     RSLGADGLKHATEMAVLNANYIKERLKPYFDLPYDRVCMHECVFSGNRQVAQSSVHTS
                     DIAKKLIDYGYHPPTIYFPLIVPEAIMIEPTETERKETLDTFCDAMIAIAREAKENPE
                     GVKNAPVSTPVSRLDEVLAARKPDVCWKKS"
     gene            complement(19952..21277)
                     /gene="gcvPA"
                     /locus_tag="PLF77_04945"
     CDS             complement(19952..21277)
                     /gene="gcvPA"
                     /locus_tag="PLF77_04945"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HPC08235.1"
                     /translation="MDYCPHTFDDVAQMQSVIGISSLDELFADIPEKFRLQHIPDIPP
                     ALSEMETLSCMGGLAQKNKMPQVTLTGAGAYHHYIPAVVGHIVGRAEFYTAYTPYQAE
                     ISQGILQAIYEYQTMIARLTGTEIANASMYDGASATAEAAVLCAKMSNRNKMIVARSV
                     HPEYRQVLRTYCWANGYTVVEIPYMPSGQIDPDALQKKLDDQTAAVIVQSPNVFGVIE
                     DIAPAVDAAHQKGALLVAGFTEGTSMGLLKPAGAQGADFVVGEGQSFGNPLNYGGPYL
                     GIFAAKEKYMRRIPGRLVGATVDKKGQRGFVLTLQTREQHIRREKATSNICSNEALCA
                     LAAGVYLAALGKNLRKLAELNVYKARYLKNKLLELPGWQDVFSAPVYNEFVLRCPDAA
                     KVNKKLKKEGIIGGYELEKDYPELKDALLFCATEMISKEDMDKVADIVK"
     gene            complement(21295..21693)
                     /gene="gcvH"
                     /locus_tag="PLF77_04950"
     CDS             complement(21295..21693)
                     /gene="gcvH"
                     /locus_tag="PLF77_04950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPC08236.1"
                     /translation="MSKPNPNDRYYSRDHEWVIYYNDGTAIIGITDYAQEMLTDIVFV
                     DLPEIGRKVVQHATVAVVESVKSVSDVFAPVSGEVIDVNTVLEETPELINQDAFGDGW
                     IVKMRLSKPEELDMLLTANQYDEMLQEDKH"
     gene            complement(21710..22801)
                     /gene="gcvT"
                     /locus_tag="PLF77_04955"
     CDS             complement(21710..22801)
                     /gene="gcvT"
                     /locus_tag="PLF77_04955"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPC08237.1"
                     /translation="MNKTPLYEKHVALKAKIIDFGGWAMPVQYTNVIDEHRTTRNAAG
                     LFDICHMGEILVKGPQALDLLQLVLTRNLADQKIGQVKLSALLNEEGGVIDDLTVYKM
                     DEHDYMLVTNATPKDRDWAWINRIQTDKKFDCALRDISNETGKLDLQGPSSEEILQKI
                     TDADLKNVRFYHFCKAHVAGFDAIVSRSGYTGEDGFEIYAPGNVVGQIWDRLMTEGAK
                     QGMKPCGLGARDTLRLESGMMLNGQDMDESVSPLEVPYGWIVDAQKEFVGSAALRAKQ
                     SSGIRKKLAGLEMTGRGIARHGYKVFCDGKEIGLVTSGTFSPTLNKAIALAFIDIDYC
                     APDTKLEIAIRDTMTAAKVVKLPFYKRLK"
     gene            complement(23289..23849)
                     /gene="def"
                     /locus_tag="PLF77_04960"
     CDS             complement(23289..23849)
                     /gene="def"
                     /locus_tag="PLF77_04960"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPC08238.1"
                     /translation="MSTKRVLALWDEAGLNARDTSVLRKKSAELPVPLDKEGREAVQA
                     LVDSFMERDDAVGLAAPQIGINKRIIVFRVKGFEDGQKKIKDIDDIEVLVNPRITQER
                     GEKVIGAEGCLSCPDVQVEVSRFPEIKVRALDAKGGKVNKKYLDYIARVVQHEIDHLD
                     GKLIVDYGDIYYPKSKQLFFEKLFKE"
     assembly_gap    24036..24135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <24136..25239
                     /locus_tag="PLF77_04965"
     CDS             <24136..25239
                     /locus_tag="PLF77_04965"
                     /inference="COORDINATES: protein motif:HMM:NF006385.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HPC08239.1"
                     /translation="CPYCSFHRITFKEPLTRRYFQALRREIRIYHEKGYQFEGVYAGG
                     GTPTVLIDELAETLNLSRELFPIRDISVETNPNHLTEKNIDVLRQCGVKRLSVGVQTF
                     NDDMLKKIGRYEKYGSGQEIAERLKYYQGHFHTLNADMIFNFPGQTPEMLEADLAVIL
                     NLNMDQVTFYPLMISDLTRDIMKKTVGHVDFSNEERFYKLIVRRLEKNYDFSSAWCFS
                     RKESLVDEYVVEFEEYAGLGSGAIGYLYGTCYSNTFDIEGYIASLEKGKLPLQASRKF
                     DLHDRMSYDFLMKLFSTKLDIKAMQKKYDGKFLKTMWKEIAAFEIVRAYRYFDTSLYL
                     TPYGRYLWVIMMREFFIAVNNFRDYCRKQAGVM"
     gene            25342..>26351
                     /gene="selA"
                     /locus_tag="PLF77_04970"
     CDS             25342..>26351
                     /gene="selA"
                     /locus_tag="PLF77_04970"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPC08240.1"
                     /translation="MDEQQKELLKQLPKIDEIILLLEKQNIYDLAPHEIVKETCRRIV
                     QNLRDKIVNAKKKLPAEFIPDAKTTAKEVEQSIKGLYRYSLRRVVNATGVILHTNLGR
                     APLCPEALERIMEVGKAYSNLEFDLNKGERGQRYDHVSSLLCALTGAEDALIVNNNAA
                     AVLLVLNTLAQKKEAIVSRGELIEIGGEFRIPEIMQKSATKLREVGTTNRTRLSDYEK
                     AVNEKTGLIMKVHTSNYRIVGFTEEASIESLVALGKNRGIPVMDDLGSGCLIDLDRYG
                     LQHEPTIRERLAAGVDVVTFSGDKLLGGPQAGIIVGKKEVLAKIKKNPLNRALRIDKF
                     TLA"
BASE COUNT         6883 a         6314 c         5971 g         6983 t
ORIGIN      
        1 gatgtcggta agcatgccat gcataagttc gtatctttaa tgcgtcactg cgtataaagc
       61 cgaatgttat tttactatca taatgttata atgattacaa attcgccgcc gtccgcggtc
      121 cttgttttca taaagaacga ttttgcaaaa cgctctagga cgcgaaagac agcgtattat
      181 tttccgatac agaaactgga aaaaatccta tccaggatct cgtcgttgat ttttttgccg
      241 gttatttcat ccagatgatc cagcgcttcg cgtaaatcga acgcgataaa ttccgctgat
      301 agaccggcgg taacgctttc tttcgcgttt tgaatatttt tcagagattg ctcgacggca
      361 tgtttgtggc gtaaattggt caggatcgcc ccggacgtgc tttctgtttc accgtcaacg
      421 gataaatcca agattgttct tttcagctct tccagtccgg agccggactt ggccgagatt
      481 tccagaaact ttgtatgagg cggaaaaagg ttcgcaatgc ttttcgtttc ccacgaacgc
      541 ggcaagtcga tcttattgac ggcgacaatg attttctccg acgtgttttt atcaatcgtt
      601 attttatctt cttctgtcaa cggtttactc acgtccagca tgattatgac aatatccgct
      661 tcggccagtt ttttccagac gagcgcaata ccctcctgct cgattacgtt ttgcggttcc
      721 cggattccgg cggtatccgt caggtgcacg ggaataccgc cgatatgaat cgtatccgca
      781 atcagatcgc gtgttgttcc cggaatatca gtgacgatcg cttttttctt tcccgcaaga
      841 gcattgagca gactggattt cccgacgttg ggctttcctg taatgaccac atgaacacct
      901 tccgaatagg ctctggcaaa gcggtaggat gtaagaagcc tctgcattct ttccattgcc
      961 tgattgatct gcggcggtat ttgcacaagc tctgcttcag cggtatcttc tgcaaaatcg
     1021 atgcttgctt ccatgagggc gagcgcttcg atcaccaacc cgcggatcgt ttcaatttct
     1081 ttggaaagcg atcctttgag ctgtgcgagc cccacggcgt aggctttttc ggattgggcg
     1141 ccaatcatcg aggccagcgc ttccgcctgg gacaaatcaa aacggttatt cagaaaagcg
     1201 cgttgtgaaa attctccggg acctgccagc cggcatccca gtttttgcaa ttggtcaaga
     1261 atggattgga tgataacagg atttccgtga cagctgattt cgacaacatc ctcgcccgta
     1321 aatgagcggg gtttgcgcat gaaagctatc aggatttcat ccagtaccgt ttcgccatcg
     1381 gcgcaaacga tatcaccatg gtaaagatga tggcttttcc agtcgcatgg acggtttgac
     1441 ggtctgaaaa tacggcgcgc cactgcaagg gcttccggtc cgctgacccg gatgacggct
     1501 attccggaca tgccgaaagg cgttgccgcg gcgactatgg tatcgctgcg aagcacggca
     1561 ggcacttccc cttattatga ttggttgata tccttctttt tctctttcgt gcgccggctg
     1621 ctgccgtttt ttgctggaag gatgataatt ttccggtact tgccttcccc gcggctttgc
     1681 gtcacgagtg attcgtcctc ctgcatgacc agatgaataa tgcgccgatc gtgagcattc
     1741 atattggcga tggataccgg ctttttggtt tttttgactt ttttctttat tttttccgcc
     1801 agatcaagca gaaaatcttc tcttctcttc cggtattctc ccgaatcgat cagaatcatc
     1861 ttgtgttcgc tgtcgatctt attgatggcc ttgttaatga tgtactggaa cgcatcaaga
     1921 ttctgtccgc gtttgccgat cagcaggccg ctttcgtcgc cctcgattcc cagaatgatc
     1981 ttttcctgag tttcttgaac atttgctttg tattccggcg atattctctg cagaatgccc
     2041 tccagcagtt cctgcgcctt gagcgacagg ggggaattgg gaatgaaagc ctcccgctct
     2101 ttttttaccg gcgatttttc ttccgatatc tcttttactt ctttttccgg cttccttttg
     2161 ggcaaaacct ttgcttcgct tttttcccgg taatggtccg ctttggaccg gggttcattt
     2221 ttttccctgg gcttgtccgt tttttctttg ggtttatccg agaaggaaaa atcgatatcc
     2281 agagccagca gggacgctct gattttcgct tttttggaaa acaatcccag aaagcctccg
     2341 gattctcctg agataatttc gatattgagc ttttcgcgag gcacgccgaa cgtctgacag
     2401 gccttctcaa tggcttcatc aatggttttt tcttctattt caataaattc agaactcatt
     2461 tttcatcctc ttttgtccaa ccggcgcttg aatcaatccg ttaatttttt attaatgtat
     2521 atctgctgtc cgatgcttat gatattattg accagccagt aaatgaccag ccccgagggg
     2581 aaattcaaaa agataaaagt gaaaatgatg ggcatggcca gcatgatctt ggcctgcatt
     2641 ggatcgccca tcgtgggtgt cattttctgc tgaataaatt gtgtcgcccc catgacaatc
     2701 ggtgtgatgt aatacgggtc tttagccgat agatcctgaa tccagaagaa aaatggagaa
     2761 tggcgcagtt caatggcata cagcagtgtg ttgtaaagcc cgatgaacac ggggatctgg
     2821 atcaggatcg gcaggcatcc gccgaaagga ttgaccttat gcgttttgta aagcgccatt
     2881 gtttcctgtc ccagtttggc ctgatcgttt ttatattttt ccctcaaagc ggtgatttgg
     2941 ggctgaatct tctgcatctc tttcatggat ttgtagctga tgtttccgag cggccagaaa
     3001 agaaccttga tgagaatggt cagaataata atggacacac cgtagttgcc cacaaactga
     3061 tgaataaatt tgatgaaaag caacagaccg atggagagcc acttcaatcc cggaatgacc
     3121 gaatcgaatt caatggcctc ttccagggac aaccccagtt ttttgagcat ggcgtagtcc
     3181 ttgggaccga taaagagatt atatttgaac gaaccggact gctccggcgg gatgatattt
     3241 tttgctcctt tcagattcac ggcaatcacg tgatcatccc gggacatgtt gaaacttgtt
     3301 aaagacggat cctgcggaat aatcactgcc atgaaatatt ttctttcaac gccgccccag
     3361 gacacatcgg gaccgatggt ttgttctttc gacacatccg ccgatttcca gcgttcgatg
     3421 gatttcgaca cgtaagccac aggaccttct ttgctgtagc cgtctacttt ttctttcggg
     3481 tccacatagt cgtaccagtt aagatacggg attttcgtta tcggcacatc ggtcagattg
     3541 gatatgttga cctccagcgt tattgaaaaa ttatcgggat tgaatatata aatcttttct
     3601 accctgacat cattgaacac gcgggaaaac accagacgct gcttcttgct gttcttaacc
     3661 atattcaact cggctgtgtc cgttttgtag acggaatccg cggcaatctc gccggatgac
     3721 tcaggaaaag taagcgtaag cggataaggc atatccggct gtaatgacac aacctcgatc
     3781 aaatcattgt ttttttctcc ggccggctgt tttttacccg taacaaaatt cttgatcgcg
     3841 ggccagatat cgccgacaca ctttgtgcat tctttctgat accccttaag ttgaaaggac
     3901 ttcagagccg cgcctttggt ggaaaacaca gcggtgtagt ttgccgtttc aaccttgatg
     3961 tcccgggatg attcttcttg tgccggaagg acgttttnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnngcc gtttcaacct tgatgtcccg ggatgattct tcttgtgccg
     4141 gaaggacgtt tttgaccccc tgtttcggcg tggaggcatc gggaaaaacc gcctgttgct
     4201 tgacgacaga attttccttt atcggggtcg gtttttgcgc tgtctgatgt gattcccgaa
     4261 ccggaaccgt ttcgggtggc acaggcggtt tggcaaaaaa aatctgatat gcaaaaagca
     4321 cagccagtga caggacaacc gccagaatgg ttctcttatc cattttcaac ctctctattt
     4381 tttacttgac gggatcgtaa ccgccaggat tcaggggatg acagcgaaga attcttttta
     4441 tgcccatcca ggaacctttt gccggtccgt acagtttgat ggcctcaatc gcatattggg
     4501 aacatgaggg ataaaaccgg cagcagggag caaaaaagaa aggtgacagg caggcttgat
     4561 acaaatgaat aaatgaaaca aaaaaattaa tgaatatctt tctgagcata aattatatat
     4621 tgttttgccg cgtgaagagt cccgttaatt catcgcatgc ttctttatac gtcaaggacg
     4681 gtatgtttcg tttagccatg atgacgacat cgtaattgtc gggaaacagg ttcttattga
     4741 ggcgaaaaaa ttctctgact agtcgtttga ttctgtttcg ttttactgcg tttcccgttt
     4801 tcttagtgac cgtaattccc agtcttttga ttccttgagt gtttaagcat gttacaattg
     4861 taaaatgtct tgaacttctc cacaccccct gattataaat tgttttaaac ctgcttcggc
     4921 tgcctatcct ttccagtttc cgataagatt gttccttcat gcatactgac ccgaccatcc
     4981 ttaaaaatgg catctgatat gccgaattta aacagcgata cgctttcttc ccttcgcccg
     5041 gcgacgattt aagacctccc ggccggcttt tgatgccatt ctgacgagaa aaccgtgtgt
     5101 tttctttctt ttggtatttg ttttattggt caaagtcttt ttcattagtg aatcctctaa
     5161 aagtaatagg gtaatgcgta aatcacagta gaatttattt gtcaaggcta aattggcaca
     5221 ccctgaaaac cggtactgcc aggaaaggta aaacggcagg aaaagattaa accggaaagt
     5281 ctctgcaata tctgtaggat gcgttagcgg ccgacaagaa tttttttaaa ccacggataa
     5341 aagagcgtca tgctcgcatc cttaacccat gtgcggtatt tctcctccag ggggttcatg
     5401 tttaatatct gggcctgaca tgatcttgcc ccgatcaggg agatcatcag actgtaaaag
     5461 cagtaaataa acatttcgat ttcgcgattt gatcctttca acgaaagctt ctcttcaatg
     5521 atacgccgca tagacgccat actcatgatg atgaaacggt agcccggtat ttcctctaaa
     5581 tttccgatat tcaccacatt cagcgcggcg atacgcaggg catcgggttt ttgcatggtg
     5641 taatcaagca tacgatcgag aaaaagctca aagccctctt tcagagacat gttttccatt
     5701 ccgtcaagcc acgcttcctg cacggaaaga gtttcatgat acaaatcttc ggccatggcg
     5761 taaaaaagat tttctttgga accaaaataa taatggatca tgggatgctc aacacctgcc
     5821 tgcttggcaa tcatgcgggt gccggcttcc ttgaagggat agaccgtgaa aacatcgcgg
     5881 gctgctttta aaatccgctc ccggacattg ccctcattgg atgaagagcg gctgcccgaa
     5941 ggtttactta atttttgcaa agatgatttc aattttttct ctatatcatt catgcgttga
     6001 tcatatcatt taccaaacca aatggtaaat aaaaatattc attttttatt tttttattct
     6061 acccctgttc gccgaaaaca atttcacgct ctattccttt gtaagttatt gtttatccta
     6121 acttataaca aaatagaatc cgacgtcaaa ctcaaaaatg aaaaaacaag ttgacaggtt
     6181 attttttttc tagtacttac caaccggtaa acaacaatcc acataaaata ttatagcaaa
     6241 attcatggag gtaaatggac tatggcaaaa tttaaagctc caagcggtgt cagcgtaaac
     6301 gactttttcg aaaaactcgt ccccgaacag ttcaaagaag tgattgccgg agcgaatctg
     6361 tcatctttaa cgggcaaaga agtcaatctt cagttcaatg tcaacgatca gcaatattgc
     6421 ctgaaaatta aagacggcac gaatctggat gtcgtcaagg gaagcgtaga caaacctctg
     6481 ctcacgttat cgctttcaga acaggtgttt cttgatgctg taaccggaaa ggcagaaggt
     6541 ttgattgacc gcttcacgga tcctgtggaa attgccgatc ccatgaggct taaaaatctg
     6601 atggatacca aaggcacttt gaatctgaaa ctgaaacagg atggaaatga gattcccgtg
     6661 accatgattt ttaacgggga agaggcgcca aaagttgata tcagtcttga tttgcaggat
     6721 tggattgcca tgcagaataa gcaaaccaca ggacagaatc ttttcatgaa cggcaagttg
     6781 aaattcgccg gcgacatgac tcttctaatg aaactgcaaa cccttatcta aaacgaggag
     6841 aagaagttac tcattacgga tcaacctgtt gcgttaaaca ccattgccgg gggtgtgtcg
     6901 aaaaaccgtt ggggggattg ggataacaca cccctggcta ttttttatga aaaccagtcg
     6961 gtcaattttt tcaggataat cttgtaacgt tcaagcccca tgaccgcatt caaggagatg
     7021 attatggaag acagagattt tgggttcggc atgtctgaag aacaaagcag tatgaaagat
     7081 actgttgcga agttggtcaa gagtatggtt tcggggaatc ccgcccatga tatggatgaa
     7141 gccagaaagc ttccccggga gatcatagac aaggtttggg aactgggagc aacggtgtcc
     7201 actgtaccgg aagaatacgg cggatacggc atggaatatt ctcccattat gaatgccatt
     7261 tttgtcgagg aacttgccgc aggcgacatg gcgctggcga tcgcgtcaac cctgcccgca
     7321 accttcatct acaccatttt gcagatgggc accgatgagc aaaaaaagaa gtatttgccg
     7381 ttgtactgca ctccgacata tcaagcctgt accgtagcga tcaatgagcc actgttcaga
     7441 ttcgacgccg tttcactgga aacaaaagcc gttaaaaacg gcagcaaata tatcatcaac
     7501 ggcaaaaagt gttttgtccc gatggccaag gattccggcc acatgctggt ggcagccgac
     7561 atcgacggga agatggattt gtttattgtg gaagggaaca atccgggtct caaaatcggc
     7621 gagcgggaaa aaaatctggg gatgtatgct ctggaaagtt ttccggtcgt gctggaaaat
     7681 tgtgaagtcc ctgatgatga ccgtgtcggc ggagataacg gatgtgatta cagcagattc
     7741 ctgcagaaaa cccgtatcgc catgtcggct atcgggaccg gcgtttcgcg cgcgtcgtac
     7801 gaatacgcgc gggaatatgc caaagaacgt atccagttcg gtgaaccgat tgccagccgt
     7861 caggccatcg cttttatgat tgcggaaatg gcctatgagg tggacgccat gaggttgatg
     7921 acctggaaag ccgcatcggc tcttgaagcc ggccgcgacg caaagaaaga ggcgtatctg
     7981 gcaaaactct acgccggcga aatgaccatg aaactgaccg atttcggtgt gcagattctg
     8041 ggcggacacg gttacattcg ggaaaatccg gtggaacgat actatcgcaa cggcagaggc
     8101 atcgccattc ttgaaggcat ggccacggta taggaggtaa gacgatgatt aatctcgaaa
     8161 tgacacaaaa gatgatggat gtaaaaagcg gcatgaacaa catggcgtca agcatgcttc
     8221 ggcccgtttc aagacattac gatgaacacg aacatgaata ccctaaagaa ctggacgtat
     8281 tccgttcaat aaaaatcatg agccgtccga aaaagaaaaa agagggcgga gaaacaaaac
     8341 cggacaaacc aggcgccaaa aaactggggg ccaatctttc gatggtcatc gtcatcgaag
     8401 agatgtgctg gggagacctg ggactgttgt tgtcactgcc gaacgcggga ttgggaaacg
     8461 cagcgattgc ggcggttgca accgacgagc agatggaaag gttcggcgaa aaatgggcat
     8521 ccatggctat taccgaaccc ggttccggtt ccgattccgg atccatcacg gccagggcca
     8581 ccttggaggg tgacgagtgg gtgctcaacg gcgaaaaaat atttgtcaca tgcgctgaac
     8641 gcgctgaagt ggcggtcatc tgggcaacgg tggacagaag cgcgggcaaa gccgggataa
     8701 aatcattcgt ggtggaaaaa ggacgccccg gattcaaact ggaaaaactg gagcataaac
     8761 tcggtgttcg cgcctcggac actggcactt acgttctcga tgattgccgc atccccaagg
     8821 acaacattct gggaagcgcg gaagtcaaac agtcaaccga aggcttcaaa ggcgctatga
     8881 aaaccttcga taacacccgc cctctggttg cgtccatggc gcttggcgta acccgggcat
     8941 ctttggattt tacacaagaa aaaatggaag caagcggcat cacgcttgat tatgaaaaga
     9001 atccgaataa tctgagtgcg atggaaaaag agttttacct gatggaggcc cacctggacg
     9061 ccatgaggct gctcacctgg cgtgccgcct ggatgatgga taacggtgaa ttcaacaatc
     9121 tggaagcgtc catgtccaag gctaaagcgg ggcgttacgg cacgttcatc tgtcagaaat
     9181 gctgcgagct gcttggacct ttgggctact cctgcgaaaa cctcgctgaa aaatggatga
     9241 gggatgtcaa aattaccgat atcttcgaag gcaccggtca aattcagcat ctaattgtgg
     9301 ccagaaatgt tctcggatat tccagcaaac aactgaaata aaattttttc tccttgctta
     9361 agaaaaatgc ctgcgcgttg tacttgcgca ggcatttttc aatttttcaa ccactcttcc
     9421 ccgtgaggaa gattaaaaaa acaccccttc tttttaacca gggcactcat tttcttataa
     9481 tttttcactg gactgtacga tattgaaacg gatatgatat gctatattga ggggtattta
     9541 tggcagatca atccaaaata ggaatggaat ttcccgcatc cactttcatc attgagaaag
     9601 acaaaattat tgaatttgcc atcgctgttt ctcaaaaaga aatgaagaat cagattcatc
     9661 ctgtttatca tgatgaaaag gccgcgaaag cagccggtta tgaaggcatt cccatacctc
     9721 ccactttcct gacgagttct tttttctgga caggcgacgg actgacaggc atggtgaccg
     9781 ccctgggaat caatttaaaa agacttctgc acagggaaga agagtacgaa tatttttgcc
     9841 ctatccatgc gggtgatatc atcacccgga aaatgaaagt tgccgatatc attaaaaagg
     9901 ggaaaggggc tagaactctt gatatgacta ttctggaagt tgaatatatc aatcagcgca
     9961 atgagttggt ggcaaaatcc cgcacaacgt taacggaaat atagaaagat gaccgggcaa
    10021 cataatacaa ttgaaatcgg gagcgagatg ccggaataca tttctcttcc gattaccaga
    10081 acccatctgg tgcgctatgc aggagcatcc ggggatttca atccgctaca tcatgatcag
    10141 acatttgcca acaccatcgg tttacagaat gtcattgccc acggcatgtt tatcatggcc
    10201 atcgcagggg aagccatcac gtcctggatc gggaacaaaa atataaaaaa attccactcc
    10261 cgttttttgg ctaagacaga acccgttgac tgggataatt ttgaaaatac aaaagacagg
    10321 gcaaccatcg tggtaaaagg aaaagtgatc agactatttg ttgaaaacgg cgaaaaatta
    10381 ttcgaatgta aaattgtcgc cgagaatctg ctgggcagca aaaaactgtc gggatcgttc
    10441 atcgcagtcg tgaccggaga acctcctcgc gatgtgaacg aataagaggt tttatgaaga
    10501 aacgtattct cgttgttgat gatgatgctg tggcgtgtga atttctcgaa gaaacgttga
    10561 aacgtgacgg ttacgacgtc accctctgca gttccgcctg ggaagctctc cggcaaaata
    10621 ttgcctccta taatttggtc atgtccgata ttcgcatgtc cggtatggac ggactgcaat
    10681 tgctggaaga actccagaaa caatcgccgg atctgccggt gatactgatg accgcttacg
    10741 gctctctgga aaccacaatg gccgctgtca acaagggagc atgggactat atcagcaagc
    10801 cattttctcc cgaagactgc cgcacgcttg tccgtaaagt gctggagtta agaaaacttc
    10861 ggcacaaggg gatgaaactg cagcccgcaa aggtcgaaga gtcaaaattc atcggttcgt
    10921 cggccatgat ggtcgacttc tataaacaaa tcgcccgcat tgccgattcc tccgccagcg
    10981 tcctgatcga aggagaaagc ggcacaggaa aggaattgac cgcacgctcg ttgcataata
    11041 tgtcctcccg ccgcgaaaag cccttcatca tcgttcattg cggagccatt cccgataatc
    11101 tgctggaatc ggaacttttc ggatacaata aaggcgcata cacaggcgcc agccatcagc
    11161 acaccgggct tctggagact gccgaaggcg gcacggtatt tctggatgaa attacggaaa
    11221 tgtccacagc gttgcagggt aaatttctgc gtttcatgca aaacggagaa cttcgaaaaa
    11281 tcggcgacaa tgccgttcgc aatgtcgagg tgcgtgttgt cgccgctgcc aataaaaaaa
    11341 tagatgacga ggtgaaaaaa gagaattttc gcctcgattt attataccga tttattgtgc
    11401 ggttgcgcat tcctccttta agagaacata aagaagacat tccgcttttg acggaagcct
    11461 tgctgaaaaa gttgggagat acaaacgtaa gcgtttcccc ggatgcaatg gagatgttca
    11521 tggcttacga ttggccgggc aatgttcggg agttggaaaa tgtccttcag caaattatgc
    11581 ttctttcgcc ttttgatgaa atcctccccg agaacctgcc ggaacgtttt catagttcga
    11641 cagacaggaa agacatacta aacggtcccg gattatcccc tctcgaagaa gcggaaaaag
    11701 aaaaaatact gcaaacgttg aaggaaacgt cctggaatca gtcccaggcg gcaaaacggc
    11761 tggacattga cagaaagact ctccggacaa agatacgtcg ttacagtctg tcttaggaaa
    11821 agcacttcag cggggaaaaa tcatttcaac gccaggacgg ttttttaatg aaaatgatct
    11881 gcatccttca atttggggaa acatagaata accattgttc cctggccgcg agaacttttt
    11941 atttccatct gaccccggtg gatcttcatg acgtcacgca cgatagccat gccgaggccg
    12001 cgcaaacctt tgctggtttt cgtgctgtag aaaggctcac agacattttc cacaacatcc
    12061 ggcgtcattc cgtaacctgt atcgctgacg gtaatggcca caaagttttc gcatggaacc
    12121 tcatgtgccg acatggtgat cgttcctccc tccggcatgg cctccagagc attctgcata
    12181 acattcatga gagctgtctg aatgcgatag cggtccacat acagaggaga caccggattc
    12241 gtgatagtga caacggattc aacgttgttt ttttgcaaga aaggctccat gagggaaagc
    12301 atttcatgga atatctgttc cacaggcagt gcttcaaagc tgggatcggg cagccgaatg
    12361 gccttgcgca cgttctgaat cacggcatcc agtctgagcg tctgctggac aatcaaattc
    12421 agtttatgtt cgacgttttc cggcaagccg cccttttcca aaagcagttt cgccaatcca
    12481 gcgatggaat gaaggggcgt ccccagatca tgcgccagat tggccgtcag ttgtcccaac
    12541 gtggttaatt gcttcaaatc cgtaacctgt ttctgcagtt ggaacacctt gatggtttct
    12601 tcatgaaccc gtttttccag atgtttggtg agttcctgat ttctttccat gacgtcctgg
    12661 atctcatcag ccatcatatt aaagtgtctg gatatctgtc cccattcgtc tcgatgaaca
    12721 atggggatac gattatcata ttgaccttcc tgaaattcat cgatagcgtg gataattaac
    12781 tttaatggtt tgccaatgat ccagcgataa ctcaggccaa gaactacaag aatgaaaatt
    12841 aataggccgg cgctgctgat aattaaaagc cggccagccc gatgtacaat ttcttcattg
    12901 gcccgcgaaa aactgatgat accaattaca atgcgcacac ttttcgtgtt tccagcaacc
    12961 ggataaagca gtcccaacgc cggtccgttt tccgtcatta ttttcatata atgcagctta
    13021 tttttcttca taactgacat cacaatatta tccgcccatt gatacggatc acgactcgct
    13081 ccggctacat agtcaaaatg tttttgaccg atatcgaaga tgtcaatcct ggccaaacga
    13141 ccgtcagcct gtaaatgatc atgaagagcg gattcaaggg ccttcagtcc ttttttggtc
    13201 ggaaaatcct ggtaaaaagt actgactgtt tcagcgacat ctttcaatct tttcgcctgc
    13261 tgttcaacta atattctttc cagagcggcg agctgaataa ttccccaaac acccatggtc
    13321 aatgcaagac cagatacaat aagaacaagc aactttgatc ttaccgtcaa cctgttgttg
    13381 aaagcggtga tctttttcag aattgtctta gccatatgcg agggtatatc aaagacaaaa
    13441 acaattatct acaaaacttg aacacagaaa cttgaaaacg acaggaaaga gcttcatccc
    13501 gtttcgtcgg gatttttttt accatagctc tataaacctg atatggttca cctcaagatc
    13561 ccctaaagtt caaattggcc aactatcagt ttgattcaaa acccgagtct attcattcag
    13621 ggaatagttt ccaaaagcca aaggcttttg aacagttcga cttgctctgc aaatttcgca
    13681 gtactcattt ctaaaaagtc ttcactaaaa agaagggata gatcacaacc tatcccttct
    13741 ttagaaccag aaaaagattt tactaactta ttttgcggcg ggagcagcgg gtacttcgga
    13801 ttccgcatct tcaacgattt tttcatcttt ctttgatgat ttcttagact ttttatcctt
    13861 tgacttcttc acgttttttt tctttgattt cttggttttg tcttttttta catcaccttt
    13921 cttgatgtca tctttttcaa tatcagcaac cttaactggc tctgatgtgt ccggtacctc
    13981 tgctttctca acggcagcaa ctgtcatgga aaatgagaag attaatgcaa caaccaacaa
    14041 tacaactttt ttcatttgaa attcctcctt gatcaatttt tattttattg caacatcacc
    14101 ctcattgagg gacacaaaca aaagttcaat aatcatgtcg ccaggacaca tttatttaag
    14161 ttggaatatt gtttctatct ttaaaagtta ggtggatacg tttgtcgttc acgcattaaa
    14221 gcaatttaca tgccagtaca aagacagtct ttatttgttg catgtatctg aaaatacaaa
    14281 agttaaattt attaagtcac gccatgaatg ttttttatta aagaaatgcg gggaataatt
    14341 ccccatcgga gaggatttat tcctcagcat agaattattg accgtttcta ctctttcatg
    14401 ttaccccatt aaaaaatcga atgatcttta tgcaaaaaga aaaaaacgaa aacgatatta
    14461 ttgtgattgg cgcaggagct gccggaatga tggcggccgg tcgtgccggt gaattgggtg
    14521 caaaagtatt actccttgaa aaaaccgatg actgcggcaa aaaaattctg gtcagcggca
    14581 agacgcgctg caacttgacc aatgcagccg aactgaacca ttttattgcc atgtatggcg
    14641 ataacggccg ttttcttttc agcgcctttc atcatttttt ccgtacggaa ctgcttgatt
    14701 ttttcaatca tcacggtctg gcgaccaagg tggaacgcgg tggacggata tttcctgtgt
    14761 ccgatgatgc tcatgatgtc gtgcgtatct tcaaaaaata cctttccgaa aataacgtaa
    14821 ctctaaaagt gaacactaaa gtaaccggca tcaccatgaa ggacgattcc gtttacggcg
    14881 tgaagacaaa ggaccaagat tatcgctgca aagctgtcgt tctggccacc ggcggggcca
    14941 cctggccttc caccggttcc accggcgacg gttacaaaat agtcgctgcg tggggacaca
    15001 acatcgtcaa accgaaaccg gcgctggtgc cgctgattgt acgggaacag gagcttgctc
    15061 aatccatgca gggagtgagc ctgcgcaacg tacgcgccac ggcttttcag ggcgaagcgg
    15121 attccatcga ttcccgaatg acgcccgatt gcatttacgg acggggagaa aaaaaatcgc
    15181 cccggcatcc cgttattgaa agccgtttcg gggaaatgct cttcacccac ttcggactgg
    15241 gcggcccgat cattttatta atgagcctgt ccgtcgttga agccctggag aaaggtctgg
    15301 tttccgtatt gatcgattta aaaccggcgc tgacccgcga acaacttcac aaacggctgc
    15361 aacgggatct ggacaatttc agcaaaagaa aaatttccgg catcctgaaa gaatatctgc
    15421 ccgccaagat gattgaacca tttattgggc ttaccggaat caacaatgaa aaactggcgc
    15481 atcagataaa cgccgcggaa agaggaaaga ttgttgagat tttgaaagcg ctgcgtttca
    15541 acattaaggc gtctctgccg ctggaaaagg caatcgttac ggctggcggt gtttcgttaa
    15601 acgaaataga tccgcgcacg atggcttcca aattgatcag cggacttttc ttttgcggcg
    15661 aggtgatgga tattgacgcc gataccggcg ggtacaattt gcaggccgct ttctctaccg
    15721 gttacctggc cggtgaaaaa gccgtcgaat acataaaggc atcttaaata tctccggcat
    15781 tacaattcag gcgcaacgga cggaaccaga caaacaaaca caatggtttc tttcccggtg
    15841 tttctgatct gatgttcttc accggccggc atgaaaatga cactctccgg atcgatcgga
    15901 ttccactggc cgttgccgta aacctcgccg gaaccggcat gaataaaaat ctcatgttcc
    15961 catggatgag aatgtttcgg cgtaaatcct cccggagcga tttcgaaaac ccgcatacaa
    16021 aaattgctcg cgccgtcagc cttgcccgta accactcgtc cggcaacgcc cctggccgaa
    16081 tccttgtcaa aatgagttgg tgcgaccgtt ttataattga tcgacttcat aagggactcc
    16141 cttgtcaacc ggttaataat gcaaacactt cgctcaatgt cggatggggc agcacccaac
    16201 ctttcaactg ctttttcgtc atttgattgg atactatcag tgcaagcgga acaatcagtt
    16261 ccgccgcgtc ttcaccgaca atggtcgcgc caatcagttt attctgatga aaaactgcct
    16321 tgacaaatcc ctgccccgtc aattctgcgc gggcaataat attggcgctg tatggagatt
    16381 tgacgacttc aacggacagg ccttcttccc cagcgcgttt ttgggtataa cccacccgcg
    16441 caatctgcgg atggctgtaa acgaccgaag ggatgcaatc ttcgttatag gcaaagtcat
    16501 ctcctccgaa aatacgttcc acagcgaatt ttgcctgctg catcgcccgg tgcgccagca
    16561 tcatcccgcc ggtaacatcg cccactgcgt aaatgccggc aacactcgtc atcatctttt
    16621 tatccacctt gattccggct tgtgtatatt caattccgca tttgtccaat tctgccgtaa
    16681 ataataacgg ttttctgcct gtgcaaagga gcgcatagtc ggcgctgatc tccaggtgct
    16741 tgccgtcttt ttgcgcgacc attgccacag aacttcccgt atccttcaag ctctccagtt
    16801 ttgtcgatgt atgaataaca acacccattc tggttaattc ctgtttgagc agatcggcgc
    16861 attcctgatc ctccagtgga atgatcgtat cgaggatttc caccatggtc acttttgtcc
    16921 ccaactcggc gaagaaggtg gcaaactccg caccgatgac actgccgccg acaatgatga
    16981 tgctcgaagg aagcgcattt aaattcaaaa cgccgtccga tgttaaaatc ctttcggaaa
    17041 gattaattcc cgacagcacc tgcggaatgg agccccaggc catcaagata ttttgagttg
    17101 ataaattttc gcttttccct gatgaatccg taaaacgaac ttccgaagga gaaataattt
    17161 ctccaacacc ttgtttgatt tccgcaccgg catcgttgag cattttttgc gcgcccagag
    17221 cggacacctt cacaatctgc tgttggtgtt ttttgacggc gggaaaatcg acggatgaat
    17281 ccgcgatgtt gatccccatc cttttgagtt ttttcagatc cgcgtaatat ttgctacagg
    17341 aaagcagagc tttggtcgga atgcagcccc ggtgtgtgca ggtcccgccc caaccttctt
    17401 tttcgatgat caggactctt tttttctttt tcgccgccat ttctgcagca gtcaatccgc
    17461 ccggtcctcc gccgataaca atcaaatcat atttttcgga cacgtttgta cctcgcaatt
    17521 tataaagaca tttttattaa agacaaatca gattcaccat tcaccctgct tttgccgcgt
    17581 gataggagct gcgcaccagc ggtccggact ctacatgctg aaaacctttt tgataagcga
    17641 tttctttgaa ctcggcaaat tcgtccggat gataatattt ggccaccggc cagtgtttca
    17701 gcgtcggctg ctggtattgc ccgatcgtca ggcgtttgca ggaaaccgct gccagatcat
    17761 caatcaatcg caaaatatca tcacggttct cgccgaagcc aatcataaaa ccgcttttgg
    17821 atacaacgga agacggaccg atatttttaa gaagcttcag cgaaacatca taattaccct
    17881 gcggccgcaa tcgcgtaaac atcggtttca caacttccat attgtggttg ataacatcgg
    17941 gctggacgtc aatcaccttt gtaagtgccg acggattccc ctgaaaatcg ggaatcaata
    18001 cttctatttt gcaatgttca acagttttgc gcagccgcct gatacaatca gcaaatacgt
    18061 gcgcaccgcc atcgcgcaaa tcatcgcggg taacggacgt gatcaccaca tactccagtt
    18121 tcattttctg cacggcatct attagatgat taatttcatt ttcgtcaatc gctgcgggtt
    18181 tgccgtgctc aacattacag taaagacagt tgcgtgtgca gatattgccc atgatcagaa
    18241 aagtcgccgt gccgcttgca aaacactccg ccatattggg acacgccgct tcctgacaga
    18301 cggtatgcaa ctgcagatcg ttcaaaactg atcgtatctt ccggcattca ttggaaaacg
    18361 gaggtcgtac ttttaaccag tcaggtttgc ggatgattga cgatttctca tttaatggat
    18421 tcatagatta ttctgcatga cgcgttatcg cacatagaaa gattaccccg gctcctgaga
    18481 gtcgcctcgc aatggcgctt acgatttttt ccagcagaca tccggtttac gcgcggccaa
    18541 gacttcgtca agccgactca ccggggttga tacgggtgcg ttcttgacgc cttcgggatt
    18601 ttccttggcc tcgcgggcaa tggcaatcat ggcatcgcag aacgtatcca gcgtctcttt
    18661 tctttccgtt tcggtcggtt cgatcattat cgcttccggc acaatcagcg ggaagtaaat
    18721 ggtcggcgga tgataaccgt aatcaatcaa cttcttcgcg atgtcgctgg tatgcaccga
    18781 gctttgagca acctgtctgt tgccggaaaa gacgcattcg tgcatgcaga cccggtcata
    18841 aggcaaatca aaatacggtt ttaatcgctc cttgatgtag ttggcattga gcacggccat
    18901 ttccgtggca tgtttcaaac cgtcagctcc cagtgaccgg atatacgtgt aggctttaac
    18961 aatgacattg aaatttccat aaaatgcgcg gacacggccg atactgtccg gaaatttttc
    19021 cgaccatttg aatttatcat ctttccgaat aatgcggggg accgggagaa aatcaagcag
    19081 cgcattgctt acgcctaccg gcccgctgcc cggaccgcct ccgccgtgcg gtgtggaaaa
    19141 ggttttgtgc aggttcagtt gaatcacgtc gaagcccaga tcgccgggtc taactttacc
    19201 taaaaaagcg ttggcattgg cgccatcgcc gtaaagcagg ccgcctttgc cgtgaacaat
    19261 ggccgccacc ttctcgatat tgcgttcgaa aagtcccagc gtgttgggat tggtcagcat
    19321 cagggccgcc acatcctccg tcatcagcga ttccagatgc tcaatatcca caccgccttc
    19381 gccgttggat ttcagttccc tgatctcaaa accgcaaaga gcgctggatg ccggattcgt
    19441 cccatgcgct gcatccggta tcagaataat tctgcgtttc tggccctttt tctcaaaata
    19501 tttttttatc atcatgacgc cggtcaattc gccatgcgcg cctgccgccg gctgaagcgt
    19561 aaattccgcc atgccgaaaa tcgcgcatag gtacttctgc atgtcataaa gcagccgcag
    19621 attgccctga gcggatttga catcagcata aggatgcaat ccggtaaaac cgggcagagc
    19681 cgcagcattt tcattaattt tgggattgta tttcatcgtg caggacccca gaggataaaa
    19741 tcccagatcg acgccgaaat tacggcgcga caaattggta taatgacgga ccaggtcaat
    19801 ttccgccact tcgggcaaat ccagatcgtc ccgcaaatat tgacggccga tcgcctgttc
    19861 aatattgatg gaaggaacat cgctcgcggg aatacccgcg gcagcgcgtc cgggtttgct
    19921 tatttcaaaa attaattcca ttccccatcc cctatttcac aatgtcagcg accttatcca
    19981 tatcttcttt ggaaatcatt tccgtggcgc agaaaagcaa tgcgtctttc agctccggat
    20041 agtccttctc caattcatac ccgccgatga ttccttcttt tttcagtttt ttatttactt
    20101 tcgcggcatc aggacaacgc aaaacaaatt cattataaac cggcgcggaa aaaacgtctt
    20161 gccagcctgg aagttccagc aatttatttt tcagataccg ggctttataa acgttgagct
    20221 cggccagttt tctgagattt ttgcccagag cggccaggta aactcccgct gccagcgcgc
    20281 acagtgcttc gttggaacaa atattggatg tcgctttttc gcggcggata tgctgctcgc
    20341 gcgtctgaag agtcaacacg aatccgcgct gacctttttt atccacggtt gcgcccacaa
    20401 gccttccggg aatgcggcgc atgtattttt ctttggcggc aaaaatcccc agatagggtc
    20461 cgccgtaatt cagcggattg ccgaagctct ggccttcgcc cacgacaaaa tcagcgccct
    20521 gggctccagc gggcttcaga aggcccatag acgtcccctc cgtaaaaccg gccaccagca
    20581 aggctccctt ctgatgagcg gcatcaacag ccggcgcgat atcttcgatc acaccgaaca
    20641 cattcgggct ttgcacaatt accgcggccg tctgatcatc caattttttt tgcagagcat
    20701 ccggatcaat ctggccggac ggcatataag ggatttccac aaccgtatag ccgttggccc
    20761 aacagtaagt ccgcaaaacc tgccggtatt ccggatgcac cgaacgggca acgatcattt
    20821 tattacgatt ggacattttc gcgcaaagca ccgcggcttc ggccgtggcg gaagcgccgt
    20881 cgtacatgga tgcattggca atttcggtgc cggtaagacg ggcgatcatg gtctgatatt
    20941 cgtagatcgc ctgaagaatg ccctggctga tttcggcctg ataaggcgta taggccgtat
    21001 aaaattccgc gcgccctaca atatgaccga cgaccgccgg gatatagtga tgatatgcgc
    21061 ccgcgccggt taacgtcacc tgcggcattt tatttttttg agccagtccg cccatgcatg
    21121 acagtgtctc catttcggaa agcgccgggg gaatatcggg aatgtgttgc aatctgaatt
    21181 tttccgggat atcggcaaat agttcgtcga ggcttgagat gccgatgacc gactgcattt
    21241 gagcaacgtc atcgaatgtg tggggacagt aatccatatc gtttttttct ttctttaatg
    21301 tttatcttcc tgcagcattt catcatactg attcgcggtc agcagcatat ccagttcttc
    21361 gggtttggac aggcgcattt tgacgatcca gccatcaccg aaagcatcct gattgatcag
    21421 ctccggcgtt tcttccaaga ccgtattgac atcaatcacc tctccactga cgggcgcaaa
    21481 aacatcggac acggatttga cggattcgac tacggccact gttgcatgct ggaccacctt
    21541 tcttccaatt tccggcaggt cgacaaaaac aatatcggtg agcatttcct gagcataatc
    21601 ggtgatgccg ataatggctg tcccgtcatt gtagtagatc acccattcat gatcgcggga
    21661 ataatatctg tcattcgggt ttggcttgga cataatgccc tccttattgt tattttaacc
    21721 gtttataaaa tggcagttta acgaccttgg ccgccgtcat tgtatcgcga atcgcaattt
    21781 ccagttttgt atccggtgca cagtaatcaa tatcgatgaa tgccagagcg atagccttgt
    21841 tcagcgtggg cgaaaacgtg ccggatgtga caaggccgat ttcttttccg tcgcagaaaa
    21901 ctttataacc gtgacgcgcg atgccgcgtc cggtcatttc caggccagcc agcttttttc
    21961 tgattccgga actttgcttg gctctcagag ccgcagaccc gacaaattcc ttttgcgcat
    22021 cgacaatcca cccataggga acctccagcg gactgacgga ctcgtccatg tcctgtccat
    22081 tgagcatcat gcctgattcc agtcgtaacg tgtcacgggc gcccaacccg cagggtttca
    22141 tgccctgttt tgcgccctcc gtcatcaacc tgtcccatat ttggcctaca acgtttcccg
    22201 gcgcataaat ttcaaatccg tcttcacccg tgtagccgct gcgcgataca atcgcatcaa
    22261 atcccgcgac gtgagctttg caaaaatgat agaagcgcac atttttcaaa tcagcatctg
    22321 ttattttctg caaaatctct tccgaagacg gcccctgtaa atccagctta cccgtttcat
    22381 tgctgatatc tcgcaatgcg caatcaaact ttttatccgt ctgaattctg ttgatccagg
    22441 cccagtctct gtctttcggc gtagcgttgg tgacgagcat atagtcatgc tcatccattt
    22501 tataaacggt taagtcatcg ataacgccac cttcttcatt gagcagcgcg gataatttaa
    22561 cctgtccgat tttctgatca gccagatttc tggtcaaaac caattgcaga agatcaagcg
    22621 cctgcggtcc tttgaccagt atttctccca tatggcaaat atcaaaaagt ccggcagcgt
    22681 ttctggttgt tctgtgttcg tcgatgacgt tggtatactg gacgggcatg gcccagccgc
    22741 cgaaatcaat aattttggcc ttcagggcga catgcttttc gtataatggc gttttattca
    22801 attttgattc atccaatctt taaaaaatat acggcaatca ctcaggtaaa aagcatgccc
    22861 gtgaaaacta taatttccgg aaaaaaatat ccctgaaaca acccgaccgc atgtcagatt
    22921 ccgggaatct ttcatccttg caaaaaggat aatacaatac ttcaaatacc ggatttagag
    22981 cataaaaccg gcttgtccgg aaaaaatcat ttcgcagggg aatatatcca ttgcacctgt
    23041 gatgattgct ttttaaaaat tactataagt aaaaatatat ttcaataatg aatactgaaa
    23101 taatcttgta tcaatcccgt gtcgtagcag atgcccttca gagcactcca ctcccgggat
    23161 cgcggcttac atttcctata gtcaacgagt tttaggatca gcgagtgtca ccccatttcg
    23221 cttcacttca gggataattc cactaacgct tcgagctgcg ctacgcttgc tctcagcgag
    23281 tgtcattatt attctttaaa cagtttttca aaaaacagtt gcttgctctt gggataataa
    23341 atgtcgccat aatcaacgat caatttcccg tcaagatgat caatctcgtg ttggacaaca
    23401 cgagcgatat aatccagata tttcttgttg actttgcctc ccttggcatc caaggcacgt
    23461 acttttattt caggaaatcg actgacctcg acctgcacat ccggacagga cagacaccct
    23521 tccgccccga tgaccttttc tccgcgttcc tgggtaatcc gcgggttgac gagaacttcg
    23581 atatcatcga tatcttttat ctttttttga ccatcctcaa agcctttgac ccggaaaacg
    23641 attattcttt tattaatacc gatttgaggc gcggccagac ccaccgcatc atctctttcc
    23701 ataaaagagt ctaccaatgc ctgaacggct tcccgtcctt ccttatccag gggcacgggc
    23761 aactcagcgg attttttcct taaaacagaa gtgtcgcggg cgtttaggcc tgcttcatcc
    23821 cataatgcta aaactctttt cgtagacatg atacttgctc aaaaattatt ttgaaaaaca
    23881 cttatcacct attccccgtt atttcaagtc atgagtcccg accgttagaa gcaaataacc
    23941 caaaggtcac tataggcgca ctgaaatttg acggacgaat tatccgatcc cgatttatcg
    24001 ggattggaaa ttatcccgcg tgcgaagcta catggnnnnn nnnnnnnnnn nnnnnnnnnn
    24061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    24121 nnnnnnnnnn nnnnntgtcc ctattgctcc tttcaccgga ttaccttcaa agaaccttta
    24181 acacgcagat attttcaggc cctgcgccgg gaaatcagga tttatcatga aaaaggctat
    24241 caattcgaag gggtttatgc gggcggcggc accccgaccg tgctgattga tgaactggcg
    24301 gaaaccttga atctttcgcg ggaactcttt cccatccggg atatatccgt ggaaacgaat
    24361 cccaaccacc tgaccgaaaa aaatatcgac gttctgcgac aatgcggcgt caagagactt
    24421 tccgtgggcg tgcaaacctt caacgacgat atgctcaaaa aaatcggacg ctacgaaaaa
    24481 tacggctccg gtcaggaaat cgccgaacgg ctgaaatatt atcagggaca ctttcacact
    24541 ctaaacgccg acatgatttt taatttcccc ggacagacgc cggagatgct cgaggcggat
    24601 ttggccgtta ttcttaattt aaacatggat caggtgacct tttatccctt gatgatttcc
    24661 gacctgacgc gggacatcat gaaaaaaacg gtgggccatg tcgatttcag caacgaagaa
    24721 cgtttttata aattgatcgt ccggcgtctg gagaaaaact atgatttttc atcggcatgg
    24781 tgcttctcgc ggaaggaatc gctggttgac gagtacgttg tggagtttga agaatacgcg
    24841 ggactcggca gcggcgccat cggctatctt tacggaacat gttattccaa cacgtttgat
    24901 attgaaggat acatcgcttc tctggaaaaa gggaaattgc cattgcaggc ttcccgcaag
    24961 ttcgaccttc atgatcggat gagctatgat tttttaatga aactattcag cacgaagctg
    25021 gatatcaaag ccatgcagaa aaaatacgac gggaaatttt taaaaaccat gtggaaggaa
    25081 atagcggctt ttgaaatcgt cagagcgtat cgttattttg atacctctct gtatttgacg
    25141 ccgtatggcc gttacctgtg ggtcattatg atgcgggaat ttttcattgc cgtgaacaat
    25201 ttccgcgatt attgccgcaa gcaggcagga gtgatgtgat attttgtgtc acttcaagcg
    25261 tatgagaaat caaaaaaaca agatgtctcc ccatgacaaa aatgaatcga tagagcaaac
    25321 tcgcatcaaa agaggataac gatggatgaa caacaaaaag aattgttgaa gcaattgccg
    25381 aagattgacg aaattattct tcttctggag aaacaaaaca tctatgatct ggcgccgcac
    25441 gaaatcgtca aagaaacctg ccgcaggatt gtgcagaacc tgcgtgataa aatcgtcaac
    25501 gctaagaaga agcttcctgc agaattcatt cccgatgcta aaacaaccgc caaagaggtg
    25561 gaacaatcaa ttaaagggtt gtaccgctac agcctgcgcc gtgtggtcaa tgccacgggc
    25621 gttattctgc acaccaacct tggacgcgcg ccgctttgcc ccgaagctct ggaaagaatt
    25681 atggaagtgg gcaaggccta ctccaatctg gaatttgatt taaacaaagg cgagcgcggc
    25741 caacgctacg atcatgtcag ttcgctgctt tgcgccctca ccggcgcaga agatgcttta
    25801 atcgtcaata acaatgcggc ggcggttttg ctggtgctca atacgctggc ccagaaaaaa
    25861 gaagccatcg tctcgcgagg cgagctcatc gaaatcggcg gtgaatttcg catcccggaa
    25921 atcatgcaaa aaagcgcaac aaaacttcgc gaagtgggaa cgaccaaccg tacacggctg
    25981 agcgactacg aaaaagccgt caatgaaaaa acaggtctga tcatgaaagt gcacaccagc
    26041 aattaccgca ttgtaggctt caccgaagaa gcaagtattg aatcgctggt tgcactgggt
    26101 aagaacaggg gcattccggt catggatgat ctgggcagcg gctgcctgat cgatctggac
    26161 cgctacggtc tgcagcacga gccgacgatc cgtgaaagac tggccgccgg cgttgacgtg
    26221 gtcaccttca gcggcgacaa acttttagga ggcccgcaag ccggaatcat cgtcggcaaa
    26281 aaagaagtcc tcgcgaaaat caaaaagaat cccctgaacc gcgccctgcg catcgacaaa
    26341 ttcacgctgg c
//
LOCUS       DAOOLB010000025        25937 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_1748_length_25937_cov_38.267676, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000025 DAOOLB010000000
VERSION     DAOOLB010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 25937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25937
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1748_length_25937_cov_38.267676"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="PLF77_04975"
     CDS             <1..295
                     /locus_tag="PLF77_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08241.1"
                     /translation="LKMAARDIGFMFSRRVFHKDLQLNNILLNPADPAQLHWIDNDLK
                     KIGRRRWAKQPKAVLEQITRNVQFCSIDEKDFFIKEIVSSWAIQKEEDDGQPS"
     gene            complement(310..999)
                     /locus_tag="PLF77_04980"
     CDS             complement(310..999)
                     /locus_tag="PLF77_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013972.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="START domain-containing protein"
                     /protein_id="HPC08242.1"
                     /translation="MTAFCRRFVLAILMVAASTGPAWSVADDGWKKIGESDGIAGFTR
                     PTAKSSVDEIRAVGIVNAPVAVVEAVIRDISIMPQYMFLCKEAALINTPEMKSAGDAV
                     YFHSVTDMPFPVSDRDAVVKSVWSVDRKTGTVYCRAKGLKTTFRQNKDIVRMPLLIIE
                     CTLVPKGADKTEVIYQVLGDPGGELPSWLVNMLTSDYGIKTIAGLRKMVSREKFRNAQ
                     KVITVTPHDQK"
     gene            complement(1039..>1440)
                     /locus_tag="PLF77_04985"
     CDS             complement(1039..>1440)
                     /locus_tag="PLF77_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08243.1"
                     /translation="RAIDMTGKAGISTQGFFIFGLPGETAASMEDNIRFALRSGLSRA
                     QFVILDVLPGSELWDSLRGKFTPNWSKESFRQPEYLPEGMTKEQLMQAQSKAFRKFYL
                     RPQILFRLLSLMDYRQIFYLLRRLKTYRILG"
     assembly_gap    1441..1540
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1541..2555)
                     /locus_tag="PLF77_04990"
     CDS             complement(<1541..2555)
                     /locus_tag="PLF77_04990"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08244.1"
                     /translation="MKIALVRPNYKSHIITPPIGLGYLSSYLKKSGIETKIIDGLKEN
                     LSPSRLMRRILKFNPDAVGITCLTAFYQEVVVLARELAKNKITTVIGGQHPTFLPYRT
                     LLESQADFVVCGEGEIPLLELAESKFCNKSIRGVYSLENLKSEQQEIIKASVVDNLDD
                     LPFPDWEQIDPREYPRAPHGAVVRGFPVGVMVTSRGCPFECTFCAAPRFCDRTIRFRT
                     PENVVAEIDYLMKNFNISEVHFEDDNFTLNADNVKKFCRLLIEKNIKIHWACPNGVRA
                     DHISEDLIVLMKQSGCYYIAYGIESVNSSILEKIKKRESVETIQRAIDMTGKAGISTQ
                     GFFI"
     gene            complement(2552..3265)
                     /locus_tag="PLF77_04995"
     CDS             complement(2552..3265)
                     /locus_tag="PLF77_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPC08245.1"
                     /translation="MNKLYVVMPAYNEAGIIEKVVQEWHAVVASVGHGSRLIVLNDGS
                     TDETSAILESIKTSHPNLTVINKTNSGHGPTCLAAYKFAVDEGADWVFQTDADGQTQS
                     GEFWRFWEKRDTCDFIIGWRARRADGPARVFISRILKGVLALLFNVSLKDANAPFRLM
                     KAERLRLYLPLIPDNFFIPNVLLSVMLTREGEKILWQEISFNPRNAGQSSIALFRFGG
                     LGIKLIIQLYKLRHLKRST"
     gene            complement(3298..>3694)
                     /locus_tag="PLF77_05000"
     CDS             complement(3298..>3694)
                     /locus_tag="PLF77_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC08246.1"
                     /translation="IDLNPQSLLAYQNRGLAYVITDDLNKALLDYNRYLALRGAYDAG
                     GSMIDTHLSSVLGNRGLIFARMNQFEKALADFNTAIKLNPYNPNNYLNRAFVFYRLGR
                     VEQAAQDVRMAELTGRTVDPSLKKMLHMP"
     assembly_gap    3695..3794
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3795..5102)
                     /locus_tag="PLF77_05005"
     CDS             complement(<3795..5102)
                     /locus_tag="PLF77_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08247.1"
                     /translation="MTALVFSGSLRLDWTNWDDNLLVYENSLVREARFIDIFTKPADY
                     NTYNPLVISSFALEWKLVGDRPFLYHFDNLILHLLCTGLVLLLFRRMGLSVWWSGVGA
                     LLFGIHPMRVESVAWITERKDVLYGFFYLSALLAYIRYIASGKAGYFLLTFCLFFLSL
                     LSKGQAVALPFSLVLLDWYFGRKMNGKAVMEKLIFFAMALLVGLLGTTFFFQNVYKTI
                     DSKAIVNAFNFFEQTVLAGYAYTIYILKFFIPYVTSTLYPVPPALHAGHWIGAITAVL
                     IFLWALAVRFQYRFVTFGLLFFTFNTLFLLMPFLANDTAYLNDRYIYMACVGLAFAAA
                     FSMQKMEEQYPIRRLPVAATAVVVLLIFSVLTIKYIPVWKNSETLWTDVIEKYPRKIS
                     MAYLNRGNDRCVQNRHNDAIHDYTAAIDLNPQSLLAYQNRGLAY"
     gene            complement(5252..6913)
                     /locus_tag="PLF77_05010"
     CDS             complement(5252..6913)
                     /locus_tag="PLF77_05010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPC08248.1"
                     /translation="MMSVSVLSVHENKSGHPQGVNKGFFSFDAFNGFDFLVLGICLIS
                     FVCSIIEARVNVDNLHWGWAYIAALDLKRGAIPHSEVIIFYGYIYTWIQSISLMLFGE
                     RLVSLGIVTGLFYSMSLFLSYRVFLRLMKRGLACLSVLLIFLIHPYAIYPAPNYYMYM
                     FELLALVYFLKYSESKYYGLLAGFFLSMSVLARYSSVIAVVPPFAVLLCAEYFVTPEK
                     KKFVMEKIGLISAGFFIPLLAFFAYLFLHSALDDFSLQNTTIVRFIGEGDNVNTYLNF
                     LIFLLQMENSLASDLRGKIFTPVLLVCLFFAIKEIRQYVFSRLREPAYKDGALFAACL
                     IAVFGYLNSIHVYETFRLVNGASLGIGVCVLAAGYCFDKVGKPLKYLIPAAGLIVAAV
                     LLSTLFFKQTTSAYYPWQRDVLFGRGIQNSSVPILKGKILTKEYNSFYEEVFDVIKPY
                     KKCCYIVNYTRDVVAFVMNDLPRVQISPVPIPGVDDISRQVQSIEEKKAVILSFKKLE
                     IPGYVTVFAKPWPAEIPWMGGGYFFIFAPERLTTDHEIYPDKEEK"
     gene            7143..7871
                     /locus_tag="PLF77_05015"
     CDS             7143..7871
                     /locus_tag="PLF77_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPC08249.1"
                     /translation="MNINNSNLHNEKIKYSLLLLSQRYNYNRWIYNNIQRFIGNNVLE
                     VGAGIGNLTDFILSKNKLTLIDIHQPYVDYLKAKYSFCDSAAFTVLQTDIQNIQSSPV
                     SGMTFDTVICLNILEHLENDRTAVENMSHLLQPKGKLIILVPALKTLYGSMDISFDHR
                     RRYNKKDLRSLIQDQNLDVLKLYYMNFPGMLGWFFNGRVLKKQELPAGQTKLFDQLVP
                     FFSFAENIVKPPLGQSLILIARKT"
     gene            7908..9311
                     /locus_tag="PLF77_05020"
     CDS             7908..9311
                     /locus_tag="PLF77_05020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08250.1"
                     /translation="MHFVFVHPPRKIPEGNIWRLINSVNPPLGLALLAALWDSAGQTS
                     EIIDAAALQLTIPEIVDQIDPSADFVGITATTPEISSAMTLARDIRNRLPQTRIILGG
                     AHPTVFHRELVKENICDMAVRGEGEEAITKLAQNTPLHLIPNLTWRNTQGEIIVNPAA
                     TDYADLDSLPFPAYEKLPMNLYHSALGAAQYQPSIGMVTSRGCPGKCTFCFSEMFGPR
                     VRFMSPLRIIEHIELLQKKYGIKEISFYDDTFTANKKNVAVLCRLMLEKKINLSWSCF
                     ARVDTVTPDLLQLMKKAGCHQIMYGFETTDEDILKNINKRITAEQFQNAIKWTRAAKM
                     NIRGAFMLGNPGETEASMQRTIAFAKNARIHFAVFNITTPYPGTAMYKEFLRNNSLLH
                     YNWDLYNLAEPVLDLDTVSDNVVRQYYYKSYRDFYLRPIFIFRHIRRIRTLSELLMHI
                     KAVLKIIPLLYRNIFSK"
     gene            9711..11042
                     /locus_tag="PLF77_05025"
     CDS             9711..11042
                     /locus_tag="PLF77_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08251.1"
                     /translation="MNSNGGILYEEMYTRLPYYLSDIPLLQKLFDSQMLDDGLYRARE
                     LSYLFDFIDVKFVELCIEHGYPHFLSLTHYLLCIATGCIIWSFCVKILNLSPLTGIGW
                     LVLFWTSPSVYFSGYFINRTGKVMVMFLTSIIFYFLCKAVVLAKEENSFPISKIDWFL
                     YSISMLIITFLDEQGVFLCMVFFTLLLIWHLIIRQKEFAIMIFICLATIILHGLYRFF
                     IAPHFIFALNGYRPDFSYQILPMILYVENLAQYLQAGFFLYLDTFRFLIGNPPLPIGF
                     ILLILLIFSPIYFMYANRQRSDNYRKYFGLALGGFLITNVLLVIMNSLMLLKHPPLNW
                     PEARRVFYWLPTTVILVMTLSVWTRLFHKSAVFKFCIVFSICLAVVGNIVALPKHKAI
                     IMRQESYVHSNIQSSSDLLKALKNLYALTDSDDPVIEKNPVFQFFKLKKKD"
     gene            complement(11318..12424)
                     /locus_tag="PLF77_05030"
     CDS             complement(11318..12424)
                     /locus_tag="PLF77_05030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HPC08252.1"
                     /translation="MKKIFFKSLLIIALLIVTFVLPVVYSTGIAVYFRLALLFFIVLE
                     SFKLFKIIIIDAKINHALSNFATTLFSFFIIFMILETVFMFVPRTHFFDYTLASKLWY
                     AKYYKPINSLGFRDNEPNYNHNVILFVGDSFTAGSGLKSVDERFSNIVGKELNKKENK
                     YTVINIGHPGADSSDEFHLMQQVIYMTKIRPEIIVLQYFGDDIAHVAAEKLKTFEGFK
                     PFVNVNAFLKPAVRGSYFLNYMYFLFPNKELTSSALSLMQQTYRDNNMLLKHQEQLKL
                     FINYARENHIQLIAVVFPVLEDIEISNSIYVNDIVNYFEVHKITTINVSRLVKNIPLQ
                     ERIINKNDGHPSKSVHASVAHEVLRKIRFNGNNE"
     gene            12621..13010
                     /locus_tag="PLF77_05035"
     CDS             12621..13010
                     /locus_tag="PLF77_05035"
                     /inference="COORDINATES: protein motif:HMM:NF025063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HPC08253.1"
                     /translation="MRNHPAFFITGKSNMPKDGLTMNPRSKPPKGVIHHSDRGAQYAS
                     HDSVEALLQHGFPISMSGKGNPYDNATTESFFKTLKVEKVYLWEYRTLEDVQIRLPFF
                     IQEFYHRKRLHSSPGYKPPVESKELFL"
     gene            complement(13242..14675)
                     /locus_tag="PLF77_05040"
     CDS             complement(13242..14675)
                     /locus_tag="PLF77_05040"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08254.1"
                     /translation="MAKVFLINPSMDILSAFGEYHKLMEPMPCIGLAYIASSCQKAGH
                     EVFMLDNFVEKKPFEDIVESVKSFGADIVGISMLTPTANSTEALGRYIKTRLPSVKVV
                     FGNLHASLFAENLINRGACDAVVHGEGEIIFPRLIEAFLNKSDISKIQGISYKGEEGI
                     VFTGMSEPIEDLDALPRPAWKLFPWRDYSFLPFVTVAKPCLAISGSRGCPFRCKFCAM
                     GYMGNLVRKRSPISIADEIEWLVKEFGARHIGFVDPIFPLDKKHAIATCRAIRAKNMP
                     EKWWWTCETRVDIVDEEMCREMKLARCKRILFGIESGVNEVLAGVGKGYTTDNVKRGI
                     VAARSAGLEISAFFMLGLPGETKETAKQTIDFALSLDIDFAKFAITIPLPGSELYDDL
                     VKQGKISQPEWESFTTFNPDPDSLPYIPEGMTGKELLQLHRMANVKFYLRPKMIFRHL
                     FVIRSIGLKMMFNGAMLILKQLFKDRK"
     gene            complement(14678..15574)
                     /locus_tag="PLF77_05045"
     CDS             complement(14678..15574)
                     /locus_tag="PLF77_05045"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC08255.1"
                     /translation="MTKKAWQAEGIVPLLYGKDKDKKRASAIGPGLHSVLGLERYFKN
                     QSFDMDFLNPVSSFYHLKSLSTKLYLHRLENYLDPIPRESIVLDAGCGVGRFTTLLAE
                     RFKKVVAFDPSLPSLKACQRHLEERGLHNVEMHWADISFLDNVGENTFDVVIATGPIC
                     YTADSLKSLKRLMRVAKPNAKIFLSVEGCLGALCSQGVEGPERLLNVLSRDPLLLEKD
                     RLINYCNRKDFKELVRNAGVKKLTIDSSHDFGDGAFWQSIDDSRLNDPTYVDMIIRTE
                     DLCRNHSVIAERARIFMAVGEK"
     gene            16146..16754
                     /locus_tag="PLF77_05050"
     CDS             16146..16754
                     /locus_tag="PLF77_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08256.1"
                     /translation="MNWDFHNILFYPPITGTTILSVDLPPHFRVIAYLIAGIVLLLGT
                     SFFFAKKYSLLNAFRKAIIIAFFSSALFYAIYTDIGWSTWLKTDIKKYWGLSTEEKSI
                     KMYNGLYVFSMEAKKLIHDDYIIYSSYFQEPRLFQYYLLPLNKRSDNHYRLEKAPYIV
                     VLKDTESQYDLAERVFTRGEITIKNVELISMFAHDAYILKRL"
     gene            16751..18208
                     /locus_tag="PLF77_05055"
     CDS             16751..18208
                     /locus_tag="PLF77_05055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPC08257.1"
                     /translation="MMLSTAIAIFLPTLLGFLIITIILRNDKETFFGERIGLSFPLGA
                     GILTLQIFLLGLMRIPLTLFNSLMPVFFELMLLSFWIWKNKIVLIPSISNPGLSLFLE
                     IKSPRNHGLKKCLFALLIIWIIAKLVSVFILTGLRPIFAWDAWANWSAGAKIFFYSKS
                     LLLDAPALEFFGGNAVDRILYYPLHNTLLQLWMSLWMGKFDDVFVKFFSPVYCLSMII
                     CLYYIAIREIGKLYALALLAIFLSSPLLSYHSIEMYSDQMLGVYLLFASLSFLKAIRQ
                     NLSFCILAGAYATIAVFTKNEALFFVLPFLLSAIVYFRHDLNKSRGKYVNLISILAPF
                     LALLPWFLFKVHYGLGFLGQLRWLRWSHELYFEYYAHSPDTSLWSFFQPDMILVYIYK
                     MISLDNFNVIFLFFPLIILAHGKISKELLHLLVPIAGYMLFFLFLYIFTAYYMGQSMC
                     LTRNILTFYPLFFFLTILLLKKDDTVLSYPAPITR"
     gene            complement(18251..19591)
                     /locus_tag="PLF77_05060"
     CDS             complement(18251..19591)
                     /locus_tag="PLF77_05060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPC08258.1"
                     /translation="MTENNRLKGKIASIPDYAFLGVILVVCLLYRIYYFGFLNPGMVQ
                     YNSDSVSYFYAVDLFRGGVDLYRTPLYPYVIKFFQYVSGDHLVRNLIFFQQALSFLSI
                     IPFYFVSKRVIGNVYVGMIATLFYGCWHPILIQNVHLNPESLCFAGSVFMLSLLVRFL
                     EKPKRSAALAIGIFPFFLIMLKPTYLILIAVMLLFLLCRFFLLRDERKILYWGLLGLC
                     FTAAGVLGYCGMNKKFNGQFVLSNIALNNTIAHLSISGAYEYGKDEEFMAVIDRTRHI
                     NYYAAPFSINNHFIDNYKRCYRNFPKDLPPTEDMIFCSVFPDRVNYSPERIKRFISHA
                     RCTVVYVKYMAQRAFDIIVAAYGNLFLLLALQSVLIMVAAVKYKKIAWAQSFCIVFVL
                     GQFFSIWLAGLDDMNRHLMPSYPFLIQIAGSFAVNIGLFLSKNKLSSKSDNAAF"
     gene            19820..>20533
                     /locus_tag="PLF77_05065"
     CDS             19820..>20533
                     /locus_tag="PLF77_05065"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPC08259.1"
                     /translation="MARRLNGKNAIITGAANGIGRASAVLFAREGANLVLADISETGL
                     RQVEDEIKGMGGNVVIRKTDVASEEEIQALINLALEKFNTIDILVNNAGIGGGLDALE
                     DQKPEEWHLVYDINVMGPVYATKHIIAHMKERRRGSIINIASVAGLLSGAGGNAYSAS
                     KAALINFTKTSACEVGAFNVRINAVCPGLIETGMTQPFFDWARSTGKENQLGKYCELK
                     RAASPEEVAWAILFLASDEA"
     assembly_gap    20534..20633
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <20634..21132
                     /locus_tag="PLF77_05070"
     CDS             <20634..21132
                     /locus_tag="PLF77_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08260.1"
                     /translation="PVSAETSNTPALSKNRATPQPAAQKANTITQAALKNGVRTCAGR
                     INQVTNFLTAGAKGGGFIMIYPTSRPDRHMTSVSMEIPLQNNTAYASASFAPGQGNYC
                     EGLYETVVYWPQTCSEVAEKKFGKFKRAGAISKNIYVRDGGATKVFLMPAGAGCVSIK
                     KELIR"
     gene            21226..24957
                     /locus_tag="PLF77_05075"
     CDS             21226..24957
                     /locus_tag="PLF77_05075"
                     /inference="COORDINATES: protein motif:HMM:NF015831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HPC08261.1"
                     /translation="MKNRFLMILALLFVGLFLSGGFLAGKVMADVVGAYVDSGGIASD
                     IDEPRGGYMFVATPGDTGTSTNVSGYYINAYFAGEVTQDPGANLFSIDYTYDPATFSY
                     TFGNAATLSLDSGSNAYLSYWDSINRIENGLLADATGVSVGSIDYTSGIYHGLTVDRT
                     ASFIDGPFLVDTNGVDTPDVAGTGVLYLDDGSMLIGTTNLANNNWNGLSADMTSATMT
                     GGTGTTTWTLSNSGAVLNDSTNGDIFTVGSYGDTTVAGTLTTGDVIYAGGNLNMGNHD
                     INNVRDINNVRDFSVTRNTTLGNGSTDSTTVTGLFNVNGDSSLRSTSGLFGMDINDSL
                     AQLGYDDGVTKRNGFMADSGRAVANSLDYTTGYQATLSLDSTDKAFISFNDGTVESGL
                     AAGPAGLMVGSTSGSTNMGMTVDTFTNDMIVGYMDTSSSEAYGLHANNSEVNLGYNNA
                     GSFRGVTVNNSLISAYSPDAMGAAGMGLGQISIPMGPSMSGAYIGYDNGTNYNGLVSA
                     GIGTAVGSINYATGEISGLATDGTNLMVGYLNGTTGATNGLMANSTEIVVNSFDSMTS
                     NEATLSLNSTDKAYLSFWDDANSIENGLLATISDVSVGSMNYNTGTSYSLNANGSSIF
                     VGSDIGGSQHGLTVDATSTTLSGGTTSSVATLADGNTSLTVDGIELFDATNNAGDTTV
                     TIGNTANSFITVSNVAGATYGETIALNGDTLVDGDLTVTGTLTVNGATFTNNGITNVN
                     NIDTDTLQTSGLATLNSAQVDTTLNVDGATTTHGITNTGNITNSGNISTATLQTSGLA
                     TLNSAQVTNNLTVNGNTTLGNAITDATTVNGTLSVSGATTTHGITNTGNITNSGDIST
                     ATLQTSGLATLNSAQINTTLNVDGATTTNGITNTGDIATDTLQTSGLATLNSAQVNTT
                     LNVDGATTLGSTLDVAGAATLNDTLNVAGDTTLASNLAVGGNTTLAGSLDVAGATTTN
                     GITNTGNITTDTLNTTGDTNVGGNLFVSGATTLTGDLVANGTNNTIGTAGVSTNTITG
                     VDNNIIATGNNFITGDMNTITATTGNTIGVTGGSTITTTADQVTALTADGSGFVANNG
                     GNVTVLNTTGHGLIVSPTETVVSGGTTSTTLTLNNTGATFRNTVTGGPARVSGVADGV
                     NENDAVNVRQYNVLKDGVEKANMGIASVSALSAIPAALPGKRVALGAGYGYFEDESAV
                     AIGVKARVLENMSVSAGIGVGISDTSSTYTANAGFSFSF"
     gene            complement(25072..>25937)
                     /locus_tag="PLF77_05080"
     CDS             complement(25072..>25937)
                     /locus_tag="PLF77_05080"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF013019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase
                     domain-containing protein"
                     /protein_id="HPC08262.1"
                     /translation="FGVAPGTSYDSNPVAMDTIKKNVIFTNCALTDNGDVWWEGMDGK
                     PPKHAIDWKGRDWPNDKNEPAAHPNARFTAPAAQCPMICPDWEKPEGVPIDIFIFGGR
                     RAGVVPLVNEAFSFDHGVFLGATASSETTAANIGAVGNLRRDPFAMKPFCGYNMGDYF
                     QHWLDMGDDLGDKAPKIFYVNWFRKTADGRWLWPGFGENSRVLKWMCERVDGKAEAVQ
                     TPIGLLPRLGGLDLKGLKIDERDIQELLRVDLDAWKAEIPDIEKHFAQFGDRLPARLR
                     KQFAELKKRLG"
BASE COUNT         7228 a         6242 c         5525 g         6642 t
ORIGIN      
        1 cctgaaaatg gcggcgcggg atattggttt catgttctcc cgccgggtct ttcacaagga
       61 cctgcaactg aataatattc tcctgaaccc tgccgatcct gcacaactgc actggataga
      121 taatgatctg aaaaaaatcg gcaggcgtcg ttgggccaaa caaccgaaag ccgtccttga
      181 acaaataaca aggaatgttc agttttgttc tattgatgaa aaagatttct ttataaagga
      241 aattgtttca tcatgggcaa tccaaaaaga agaggatgac ggacagccgt cctgaaaaac
      301 agccatcgtt catttttgat catgaggcgt tacggtaatc accttttggg cattcctgaa
      361 cttttcccgc gacaccattt tcctgaggcc ggcgatcgtt ttgatgccgt aatcgctggt
      421 cagcatgttg accagccagg aaggcaattc acctccggga tcgccgagga cctgatagat
      481 cacttccgtc ttatccgcac ctttcggcac cagcgtgcat tcaatgatca aaagcggcat
      541 ccgcacgata tccttatttt gcctgaatgt tgttttcagt cctttcgcac ggcaatacac
      601 ggtccccgtt ttcctatcga cagaccatac ggatttgacg acagcgtcac ggtcgctgac
      661 ggggaaaggc atgtcggtga cgctgtggaa atagacggca tctcctgcac ttttcatctc
      721 cggggtattg atcagggccg cctctttgca taaaaacata tactgcggca tgatggaaat
      781 atcgcggata acggcctcga ccaccgcgac cggagcattc acaataccga ccgcccttat
      841 ttcatcaaca gaactcttcg ctgtcggacg cgtaaaaccc gcaatgccgt ccgattctcc
      901 gattttcttc cagccgtcgt cggctacgga ccatgccggc ccggtactcg ccgccaccat
      961 caaaatcgcc agtacaaacc gtctgcaaaa tgctgtcata gttatttccg cttcacgcaa
     1021 tcattctttc ccgccattct atccgagaat tctataggtt tttaatctcc tgagcaaata
     1081 aaatatctgt ctgtaatcca ttaaagaaag aagtctgaac aagatttgcg gtctcaaata
     1141 aaatttccgg aaggccttgg attgcgcctg catcaattgt tctttggtca ttccctccgg
     1201 aagatattcc ggttggcgaa aactctcttt tgaccagttc ggcgtgaatt tgccccgcag
     1261 actatcccac agctccgacc cgggcagaac atccaggatg acgaattgcg ctcttgacaa
     1321 tcccgacctt aaggcaaaac ggatattgtc ttccatcgag gccgccgttt ctcccggcag
     1381 cccgaaaatg aaaaaaccct gcgtggaaat gccggctttg ccggtcatat cgatggcgcg
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaatgaaaaa accctgcgtg
     1561 gaaatgccgg ctttgccggt catatcgatg gcgcgttgga tggtttcgac cgactccctt
     1621 ttcttgattt tttccagaat ggacgaatta acggattcga tgccgtaagc gatgtaatag
     1681 catccgcttt gcttcatcaa aacaataaga tcttccgata tgtgatcggc gcggacgccg
     1741 ttcgggcagg cccagtgtat cttgatgttt ttttcaatca ggagacggca aaacttttta
     1801 acgttatcgg cgttcagcgt gaaattatca tcttcgaaat gaacttcgct gatgttgaaa
     1861 tttttcatca gataatcgat ttcggcaacg acattttccg gtgttctgaa tctgatggtt
     1921 ctgtcgcaga atcggggagc ggcgcaaaag gtgcactcaa agggacaccc ccggctggtg
     1981 accataacac ctacgggaaa gccccggaca accgcgccgt gcggggcgcg gggatattcc
     2041 cgcggatcaa tctgttccca atcggggaaa ggcagatcat cgagattgtc caccacggac
     2101 gctttgatga tctcctgctg ttcgcttttg aggttttcca gcgaatacac cccccgaata
     2161 ctcttgttgc aaaatttact ttcggccaat tcaagcagcg gaatttcccc ttcgccgcaa
     2221 acgacaaaat cggcctgcga ctccaacagc gtgcggtacg gcagaaacgt aggatgctga
     2281 ccgccgatga cggttgtgat tttatttttt gcaagttcgc gggccaacac gaccacttct
     2341 tgataaaacg cggtgagaca ggtaatgccc accgcatcgg gattgaattt cagaatccgg
     2401 cgcatcagcc gcgacgggct gagattctct ttcagaccat caataatttt tgtctcgatg
     2461 ccggactttt tgagatacga cgagagatac cccaggccga tcggcggtgt tatgatatgg
     2521 cttttatagt ttggtcggac cagtgctatt ttcatgtcga acgcttcaga tgtctcagtt
     2581 tatataactg tataatcagt tttattccca acccgccaaa ccgaaacaga gcaatcgacg
     2641 attggccggc attgcgcgga ttgaaagaaa tttcctgcca taagattttt tctccttccc
     2701 gggtcagcat tacggacaga agcacattgg gaataaagaa attgtcggga attagcggca
     2761 ggtacaggcg caaacgttca gccttcatta aacggaaagg ggcgttggca tcctttaacg
     2821 aaacattgaa cagaagcgcc agcactcctt tcaggatccg cgaaatgaaa accctggccg
     2881 ggccatccgc ccgtcttgcg cgccaaccga taataaaatc gcaggtgtcc cgtttttccc
     2941 agaacctcca gaattcgccg gattgcgtct gaccgtcggc gtcggtttga aaaacccaat
     3001 ccgctccttc atcgacggca aatttatagg cagccagaca cgtcggtccg tgaccggaat
     3061 tggttttgtt gatgaccgtc aggttcggat ggctggtttt gatgctctcc agaatggcag
     3121 atgtctcgtc cgtgctgccg tcgttcaata caatcaatct gctgccgtgg ccgacagaag
     3181 cgacaaccgc atgccattcc tgaactacct tttctattat ccccgcttcg ttgtaggccg
     3241 gcatgacaac atagagttta ttcatgatca tcctgttttc ataagactaa aaatccatta
     3301 cggcatgtgc agcatttttt taagtgacgg gtccaccgta cggcccgtca actccgccat
     3361 ccgcacatcc tgagcagcct gttccacgcg accgagccgg taaaaaacaa aggccctgtt
     3421 taaatagttg ttcggattat acggattgag ttttatggcc gtgttaaaat cagcgagcgc
     3481 tttttcaaac tgattcatcc tggcaaaaat cagcccccga ttgcccagca cgctggagag
     3541 atgggtatca atcatgctcc caccggcatc gtaggcccct ctcagcgcca aatagcggtt
     3601 atagtccaga agggctttgt tcaaatcatc cgttatcaca taggcaaggc ctctgttctg
     3661 atatgccaga agactctgcg gattgagatc aatcnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnataggc aaggcctctg ttctgatatg ccagaagact ctgcggattg
     3841 agatcaatcg ccgccgtata gtcgtgtata gcatcattgt gccggttttg cacacaccgg
     3901 tcattgccgc ggttgagata agccatggat attttacggg gatatttttc tataacgtcc
     3961 gtccagagtg tttcgctgtt tttccagacc ggaatatatt taatcgtcag gacgctgaag
     4021 atcagcagaa ccaccacagc ggtcgctgcc acgggcaggc ggcggatagg atactgctct
     4081 tccattttct gcatggaaaa ggcagcggca aacgccagtc caacgcaggc catgtagata
     4141 taccgatcgt tcagataggc cgtatcgttc gccagaaatg gcatcagaag gaaaagggtg
     4201 ttgaatgtaa aaaaaagcag cccgaatgta acaaaccggt attggaaacg gacagccagg
     4261 gcccagagaa aaatcaaaac ggctgtaatc gcaccgatcc agtggcccgc atgcagagcc
     4321 gggggaacag ggtacagcgt ggatgtcaca tagggaataa aaaacttcag aatatagatt
     4381 gtataagcat aacccgccag caccgtctgt tcaaagaaat taaaagcatt gacgatcgcc
     4441 ttgctgtcta tggttttata aacattttga aagaaaaacg ttgtccccag caaaccaacc
     4501 agcaaagcca tggcgaagaa aattaatttt tccattaccg ctttcccgtt catctttctt
     4561 ccgaaatacc agtccagcaa aaccagcgag aagggaagtg caaccgcctg ccccttggaa
     4621 agcagcgaca aaaaaaataa gcaaaaggtt aacaaaaaat aacctgcttt gccggaagcg
     4681 atgtaacgaa tataagcgag caaagccgac aaataaaaaa atccatagag aacatcttta
     4741 cgttcggtga tccatgcgac ggactccacc cgcatcggat gaatgccgaa aagcaaggca
     4801 ccgacaccgc tccaccagac agagagaccc atcctgcgga aaagaagcag aacgagcccc
     4861 gtacagagaa gatggagaat gagattgtcg aagtgataaa ggaaaggtct gtctccgacc
     4921 agtttccatt ccaaagcgaa agaagaaata accagaggat tatacgtgtt atagtcggcc
     4981 ggcttggtga atatgtcgat gaaccgtgct tcacgaacca gggaattttc gtaaaccaat
     5041 agattgtcgt cccagttggt ccaatccagt ctcaggctgc cggaaaaaac aagcgcagtc
     5101 acggcaagga ctcccaaaaa tataagacat ttgcgctttt ccattttaca gcgatcccca
     5161 tttatcccgg atgaactcga tatcgttaga cttataacat atgcgcaagg acatcacctg
     5221 tcaaagtaac atttttttaa agtgcaataa cttatttttc ttctttatcc ggatagattt
     5281 catgatcagt cgtcaggcgc tcaggtgcaa aaataaagaa ataaccgcct cccatccacg
     5341 gaatttcagc aggccacggt ttggcaaata ccgtcacata tccgggaatc tcaagttttt
     5401 taaatgacag aatgaccgct tttttctctt caatggattg cacctgccgg gatatatcat
     5461 cgacgccggg aatgggtacg ggagagatct ggaccctggg caaatcattc attacaaacg
     5521 caaccacatc tcttgtgtaa ttgacgatat aacaacattt tttatagggt tttataacat
     5581 caaacacttc ttcgtaaaag ctgttgtatt cttttgttaa aattttacct ttcagtatcg
     5641 ggacgctgct gttttggata cctcggccga agagaacgtc cctttgccag ggataatacg
     5701 ctgatgtcgt ctgtttgaaa aacaacgttg acagcaatac cgccgcaacg attaatcccg
     5761 ctgccggaat cagatatttg agcggttttc cgaccttatc aaaacaataa ccagccgcca
     5821 gcacacagac gccgatgccc agagaagcgc cgttcaccag gcggaatgtt tcataaacat
     5881 ggatggaatt aagataaccg aagacggcga tcagacaagc ggcaaacaga gcaccgtctt
     5941 tatatgccgg ctcgcgaagc ctgctgaaga cgtactgacg aatttctttg atcgcaaaaa
     6001 acagacaaac cagcaacacc ggcgtaaata tttttccgcg aagatccgag gccagactgt
     6061 tttccatctg gagcaggaaa atcagaaagt tcaaatacgt attgacatta tctccctcac
     6121 cgatgaacct gacaatcgtt gtattttgaa gggaaaaatc atccaacgcg gaatgcagaa
     6181 aaagatatgc aaaaaaagcc agcaagggga tgaaaaaccc ggcgctgatc aatccgattt
     6241 tttccatcac gaatttcttt ttctccgggg taacgaaata ttccgcacaa agcaaaacgg
     6301 caaaaggcgg aacaacggca ataacggacg aataccgcgc cagaaccgac atgcttaaaa
     6361 aaaatcccgc cagcaggccg taatatttgc tttcggaata tttcagaaaa tagacaagcg
     6421 ccagcaattc aaacatatac atataataat taggcgcggg ataaatggca taagggtgaa
     6481 tcaggaaaat cagcagcacg gaaaggcagg cgagacccct cttcatcaat ctgagaaaaa
     6541 cacgataaga aaggaataag gacatggaat aaaacaggcc cgtgacaatg cccagactca
     6601 ccagcctctc accgaacaac atcaacgaaa tgctctgaat ccatgtataa atatagccgt
     6661 aaaagataat gacttcggaa tgcgggatgg ctcctctttt caaatccagc gcggcaatgt
     6721 aggcccagcc ccagtgcaaa ttgtccacgt tcactctggc ttctatgata ctgcagacaa
     6781 aactgatcag gcatatgcca agaaccagaa aatcaaaccc gttgaatgcg tcaaaagaga
     6841 aaaacccttt attgacaccc tggggatggc ctgatttatt ttcgtgaacg gataataccg
     6901 aaacgctcat catggctttt gaggcaacct cttcttaggg ttcggtttag tcccgggata
     6961 caaagagaga tgtttatcag accgttcaat tccggcttga caaatcacca ttcccgttgt
     7021 tttttaaaag aaattttaaa acgtttaaat tatttaattt ttcgcagacg attgcaacat
     7081 ttttttagat gacctttatc catgaataag gtaatgaaga atcatccact taaaaacggg
     7141 gaatgaacat caataattca aatctccata atgaaaaaat aaagtattca ttgctgctgt
     7201 tgtcccaacg atataactac aatcgctgga tatacaacaa catacaacgt tttatcggaa
     7261 ataacgttct ggaagtcggt gcgggaatcg gcaatctcac ggactttatc ctttccaaaa
     7321 acaaacttac gctgatcgac atccatcagc cctatgtgga ttatttaaag gcgaaatact
     7381 ctttttgcga cagcgcggcc ttcacggtcc tccaaacgga tattcaaaac attcaatcat
     7441 cgcccgtctc cggcatgacg ttcgacaccg tgatttgcct gaatattctc gagcacctcg
     7501 aaaacgaccg gacggccgtt gaaaacatga gtcatctttt acagccgaaa gggaaactca
     7561 tcatcctggt ccccgccctg aagacgcttt acggctcgat ggatatttct tttgatcacc
     7621 ggcggcgcta taataaaaaa gatttaagat cgctcatcca ggatcaaaat ctggacgtgt
     7681 tgaaacttta ctatatgaac tttccgggca tgctcggctg gttctttaac ggacgtgtac
     7741 tgaaaaaaca ggaactgccg gcgggacaga ccaaattatt tgatcaactg gtgccgtttt
     7801 tcagctttgc ggaaaacata gtcaagccgc cgctgggaca gtcgctgatt cttattgccc
     7861 ggaaaacgtg atttgcacgc aataaaacaa ctccgatgat tcgacctatg cattttgtat
     7921 ttgttcatcc gccgcgaaaa ataccggaag gcaatatctg gcgtctgatc aattccgtca
     7981 atccgcccct cggattggcg ttgctggccg cgttatggga cagtgcgggg caaacatcgg
     8041 aaatcatcga cgcggcggct ttacagttga ccattccgga aatcgtcgat cagatagatc
     8101 cctcggcgga ttttgtcggt atcacggcaa ccacgcctga aatatcgtct gccatgacgc
     8161 tcgcccggga catccgcaac agactgcctc aaaccagaat catcctgggc ggcgcacatc
     8221 cgactgtttt tcatcgggaa ctcgtcaaag agaatatctg cgatatggcg gtgcgcggcg
     8281 aaggcgagga ggcgataaca aaactggccc aaaacacacc gttgcatctt attcccaacc
     8341 tgacctggcg aaatacgcag ggcgaaatca tcgtcaatcc agcggcgaca gattatgcgg
     8401 atctggacag cctgcctttt cccgcttatg aaaaacttcc gatgaacctc tatcattcag
     8461 cgctgggagc ggctcaatat caaccttcca tcggcatggt cacatcccgt ggctgccccg
     8521 gaaaatgcac attctgtttt tccgaaatgt tcggtccgcg agtcaggttt atgtcgccat
     8581 tacgcattat tgaacatatc gaactgttgc aaaaaaaata cggcatcaaa gagatttcct
     8641 tttatgacga cacattcacc gcaaataaaa aaaatgtcgc cgtattgtgc cggttgatgc
     8701 tggagaaaaa aatcaatctc agctggtcat gttttgcacg cgtggatacc gtcacaccgg
     8761 acttgctgca gttgatgaaa aaagcgggct gccatcaaat catgtatggt tttgaaacca
     8821 cggacgaaga tattctaaaa aacatcaaca agcgaatcac cgcggagcaa tttcagaacg
     8881 ccatcaaatg gacaagagcg gcaaagatga acatccgcgg cgcttttatg ctcggcaatc
     8941 cgggagaaac ggaagccagc atgcaaagaa ctatcgcttt tgctaaaaac gcacgaattc
     9001 attttgccgt tttcaatatc acgactccat atccgggcac cgccatgtat aaagaattct
     9061 tacgcaataa ttcccttcta cattataatt gggatctgta taatctggca gaacccgtgt
     9121 tggacttaga caccgtatcg gacaacgtcg tcagacaata ttattataaa agctaccgtg
     9181 atttctatct gcggccgatc tttatttttc ggcatatccg caggatacgc actctttccg
     9241 aactgttgat gcacattaaa gcagtactaa aaataattcc ccttctctat agaaatattt
     9301 tttcaaaata aatttcaggt tataaattaa aaatcttttc tgcaaaaaaa cagcagcgaa
     9361 cattccggaa aagtctttct ggtattcccg gaagccaaag cagctataca gttcaaccat
     9421 aatttcattg acaaaaaacg gcattctccc tatatattca caacactcaa ctggaaataa
     9481 cactctatca caaagcatca tcagccaggc aatgcgccat gggacagata accgatatgc
     9541 ccgggaccga attagttttt taaccgctgt attgaaacat caagcacagc cgtcggaaca
     9601 tttatttcat gaaattatgt aaacttataa aatttccgga tcattttaag gttgacatct
     9661 cttgctttca cgcgtttttg ttaagttgtc tgattgcact gatttccatc atgaactcta
     9721 atggcggtat tctgtatgaa gaaatgtata ctcgtctgcc ctattatttg tcagatattc
     9781 cattattgca gaagttgttc gacagtcaaa tgctggatga cggtttatac cgtgcacggg
     9841 aattaagcta tttatttgac tttatcgacg ttaaatttgt tgaactctgc atagagcacg
     9901 gttacccgca ttttttatct ttaacacatt atcttctgtg tatcgcaaca ggttgtataa
     9961 tttggtcctt ttgcgtgaaa atattaaacc tcagtccttt aacagggatc ggctggcttg
    10021 tactgttttg gacttctcca agcgtctatt tcagcggata ttttattaac aggacaggaa
    10081 aagttatggt gatgtttctg acgtccatta tattttattt cctgtgtaaa gcggttgttc
    10141 ttgcaaagga agaaaatagc tttcctatct caaaaattga ttggtttctg tattccatat
    10201 caatgctgat cataacgttt ctggatgagc agggagtatt cctctgtatg gtgtttttta
    10261 cactcctgct tatatggcac ctgattatcc gccagaagga atttgccatt atgattttca
    10321 tttgtctggc aaccattatc cttcacggat tgtatcgatt ttttatcgct ccgcacttca
    10381 tatttgctct caacggatac cggccggatt tttcttatca gatattgccg atgatactat
    10441 atgtcgaaaa tttagcgcag tatctgcagg cgggattctt tttatatctt gacacattcc
    10501 gatttttaat cggcaatcct cccctgccca tcggctttat tctcctcata ttgcttattt
    10561 tttctccaat ctatttcatg tacgctaacc gtcagaggtc cgacaactat cgaaagtatt
    10621 tcggtctggc gctgggagga tttttaataa cgaatgtatt gctggtcatc atgaattcac
    10681 tcatgctgct aaaacatccg ccgctaaact ggccggaagc cagaagagta ttctattggt
    10741 tgccgacaac agttattctt gtaatgacac tttctgtgtg gacccgtctt ttccataagt
    10801 ccgctgtttt caaattttgc atcgtttttt caatctgtct tgccgttgtt ggaaatattg
    10861 tggcgcttcc caaacacaag gccattatca tgaggcaaga aagttacgtt cactctaaca
    10921 ttcaatcaag ctcagattta ttaaaggcac ttaaaaacct ttacgcactg acggattccg
    10981 atgatcctgt gattgaaaaa aatcctgttt ttcaattttt taagcttaaa aagaaagact
    11041 agcggaataa ccttatcatg cacaatggaa ggggagaagc ctgcgatacg agacaatatc
    11101 cccagagata agcagacatg gaaagtttga tctgaaagtt ccgatgtgaa aatgacgtct
    11161 tttcgtcaag aggattgttg attaaaaaag gttttagata aaagaagaat ttatcaattc
    11221 ttcttaaaca tgatcaatag tcttttcaga acaaaaccat ggctatcggc agcaatttat
    11281 tttgttctcc aacaaaaatc ggatcatcgt aaaatattta ttcattattt ccattgaatc
    11341 taatctttct caaaacttca tgagcaacgg atgcatgaac tgattttgaa ggatgcccgt
    11401 catttttatt tattattctt tcttgcaggg gaatattttt aaccaatcga gagacgttga
    11461 tagtagttat tttatgaact tcaaaataat tcacgatatc attgacatat atagaattgc
    11521 tgatttcaat atcctcgagg acaggaaaca ctaccgcgat aagttgtata tgattttctc
    11581 gagcgtaatt gatgaataat ttaagctgct cctggtgctt taataacata ttattatccc
    11641 gatatgtttg ttgcattaaa gaaagagctg aagacgtaag ttctttattg gggaaaagaa
    11701 aatacatgta attaagaaaa taagagcctc ttaccgcagg ctttaggaat gcattcacat
    11761 ttacgaaagg tttaaaacct tcaaacgttt ttaatttttc cgccgcaaca tgcgcaatat
    11821 catcgccgaa gtattggagc actatgattt cgggcctaat ctttgtcata taaatgactt
    11881 gttgcatcaa atgaaactca tcagatgaat ccgctcccgg gtgtcctata tttattacag
    11941 tatatttatt ttctttttta tttaattctt tgccaacaat attggaaaat ctttcatcta
    12001 cagatttcaa tccactgccc gccgtaaatg aatcgcctac aaataaaatt acgttatgat
    12061 tgtaattagg ctcgttgtct cgaaaaccta aagagttgat cggtttataa tattttgcat
    12121 accacaattt ggacgctaaa gtataatcaa agaaatgcgt tcgtggaaca aacatgaaga
    12181 cagtttccag gatcatgaaa ataataaaaa atgaaaataa agttgtggca aaattcgaca
    12241 atgcatgatt gatctttgca tcgatgataa taattttgaa caatttgaaa ctttcaagga
    12301 ctataaaaaa cagcagagct aatctgaagt aaacagcaat accggtagaa taaaccacag
    12361 gcaaaacaaa agtaactatc aacagggcaa ttatcaacaa acttttaaaa aatatttttt
    12421 tcatagtaac aaattagact gcccccccac ccggattgaa caaaatatgt tagactagtt
    12481 ggcaggaacc agattccctt tatgggaaag aaaggaactt gccatgaaac actgcagagc
    12541 atttgctgtt gaactcaagc gccagcccgt tgaagaactt ttaagcggta tgagtacacc
    12601 agcgcaactg attcgccgtc atgagaaatc atccggcctt ctttatcact ggaaagagca
    12661 atatgccaaa ggacggttta acaatgaacc ctcgaagtaa gccgcccaag ggtgttattc
    12721 atcattcgga tcggggcgcc cagtatgcct cccatgattc cgtagaagcg ctcttgcaac
    12781 atggctttcc gatcagcatg tcgggcaaag ggaatcccta cgataatgct accacggaaa
    12841 gctttttcaa aacgctcaag gtcgaaaaag tttacctttg ggaataccga accctggaag
    12901 atgttcagat tcggcttccc tttttcatcc aagaatttta tcaccgcaaa cgtctgcatt
    12961 cctctcctgg atacaaaccg ccggtggaat ctaaagaact ttttttataa aaaccaaaaa
    13021 cccgcgccga ttgctctaac ccgatctatc taaccgcagg ggctcaatcc agagactctt
    13081 ccaacctatc ttgagcggca tgcaatccgg aaggataacc tcatgccctt gccgtctggc
    13141 ctccgatcca agccaggcaa gcgcctgaag aagcagataa tcatcctgct cattcatgta
    13201 atgccatata tatttcggtg gccgaacaaa taataatcgc attacttcct gtctttgaac
    13261 agttgcttca atatcagcat cgctccatta aacatcattt tcaaaccgat tgaacgtatc
    13321 acaaaaagat gccggaatat catctttgga cgcagataaa actttacgtt tgccatccga
    13381 tgcagttgca agagctcttt tcccgtcatg ccctcaggaa tatagggaag cgagtccgga
    13441 tcgggattga aggttgtaaa gctttcccat tccggttgtg atattttccc ctgcttcacc
    13501 aaatcatcat agagttccga accgggaagc ggtatcgtta tcgcgaactt tgcaaaatca
    13561 atgtccagcg atagcgcaaa atctatcgtc tgttttgccg tttcttttgt ctcgcccgga
    13621 agcccgagca taaaaaaagc ggagatttcg agtcccgcag accttgcagc aacgatcccc
    13681 cgcttcacat tatccgttgt atacccttta cccacaccgg ctaaaacttc attaactccg
    13741 ctttcaatgc cgaacaagat tcgcttacat cgcgccagtt tcatctcccg gcacatctcc
    13801 tcatcgacaa tgtccactcg tgtctcgcat gtccaccacc atttttcagg catgtttttt
    13861 gccctgatgg cccgacatgt tgcaatcgcg tgttttttat caagtggaaa aatcggatca
    13921 acaaatccga tatgtcgggc accgaattcc ttcacgagcc attcgatctc atcggcaatc
    13981 gatatcggac tgcgttttct gaccagatta cccatataac ccatcgcgca gaacttacac
    14041 cggaacggac agcccctgct tcccgaaatc gccaaacagg gtttggcgac tgtaacaaac
    14101 ggcaaaaaag aataatctct ccagggaaac agtttccaag cggggcgggg aagagcgtct
    14161 aaatcttcta tcggctcgga catccctgta aaaacgattc cctcttcgcc cttataggat
    14221 atcccctgaa tttttgaaat atcgctcttg tttaaaaatg cctcgatcaa ccttggaaaa
    14281 ataatctccc cctcgccatg cacgaccgca tcgcatgctc cccgattgat gagattttca
    14341 gcaaatagcg acgcatgtaa atttccaaaa acaactttga cagacggcag ccttgttttg
    14401 atataacgtc ctaaagcttc tgttgaattt gcggttggcg tcaacatact tattccgaca
    14461 atatcagcgc caaaagattt gacagattca acaatgtctt caaaaggttt cttttccaca
    14521 aaattgtcga gcatgaatac ttcatgaccg gctttttgac agctcgaagc gatgtacgcc
    14581 aaaccgatac acggcatggg ctccattaac ttgtgatact ctccaaaagc ggacagaatg
    14641 tccatagaag ggtttattaa aaagacttta gccatcttca cttttcccca acagccataa
    14701 aaatcctggc ccgttcggca atcacggaat gattccggca aagatcctcg gttcgaataa
    14761 tcatatctac atatgttgga tcattcaggc gcgagtcgtc aatggattgc cagaatgccc
    14821 catccccaaa atcgtgcgaa ctgtctatgg taagcttctt cacacccgca ttccggacga
    14881 gttctttaaa atcttttcgg ttacaataat tgatcaaacg gtccttttca agcagaagcg
    14941 gatctctcga aagaacgttc agaagccttt ctggcccttc aacgccctgc gagcataatg
    15001 cacccaggca tccttcaacg gataaaaata tttttgcgtt tggtttcgca acgcgcatca
    15061 aacgcttaag ggattttaga gaatcggccg tgtaacaaat gggtcccgtc gctatcacga
    15121 cgtcaaaagt attttccccc acgttgtcca aaaacgaaat atccgcccag tgcatctcaa
    15181 cattatgaag cccgcgctct tccagatgac gctgacatgc tttcaacgac ggcaatgaag
    15241 ggtcaaacgc aacaaccttc ttgaatcgtt cagccaaaag ggtggtaaat cttccaacac
    15301 cgcaaccagc gtcaagaact atagattccc ttggtattgg atcaaggtaa ttttcaaggc
    15361 gatgaagata tagtttggtc gaaagactct tgaggtgata aaaagaagag accggattga
    15421 ggaaatccat atcaaaggat tgatttttga aatatcgttc caatcctaaa acactgtgca
    15481 gtcccggccc gatagctgat gcccgttttt tatctttgtc tttcccgtat aacagaggaa
    15541 ctattccctc agcctgccaa gccttttttg tcaatatcac tgctccttat ccgtttccaa
    15601 gagctcgttt tgtcacactt tacaacaata aatcatagga gatgaaataa tcaatgtgca
    15661 aaggcaattt tataagaaag gttttaacaa tacttggacg tcttgcgctt ttccctgtta
    15721 aatcaacagg agacacagcg ccctccgttc ttttttaaaa aaaagatttc tccccaccta
    15781 gagacgtaaa acaagggcac aactacatta atggggtttc ggcctcggct tgggagaggg
    15841 tctccacacc tgtattttcc ccctccttat ggttttattt ctatataaaa ataaaggccg
    15901 agtcaataca tattatgaga aacattagcc attttcatca tcatctgcaa ccaaaagcaa
    15961 aacttaaaaa atcacagagg ggaaatgaca taatatcaag ctttttttac acatagaaac
    16021 tattccgggc acctatcatt ttcttcaaac aagcgctcaa tgtatgagtg ttgtattgat
    16081 ttaaaaacca aaaggaattc cttactattg acttatattg gttttttgct ataaaaacaa
    16141 aaactatgaa ttgggatttt cataatattc ttttctaccc gccgataacc ggaaccacta
    16201 ttttatcggt tgatctgccg ccgcatttca gagtaattgc atacttgatt gcaggtattg
    16261 tcttactcct gggaacatca tttttttttg caaaaaaata ttctctgctt aacgctttca
    16321 gaaaagctat cattatcgcg tttttctcct ctgcgctttt ctacgcaatt tatacggaca
    16381 tagggtggag tacatggctg aaaaccgata ttaagaagta ctggggtctc tcaacagaag
    16441 agaagtctat taaaatgtat aacggcttgt atgtattctc tatggaagca aaaaagctga
    16501 tccatgatga ctacataata tattcttcct attttcaaga accgagactt tttcaatatt
    16561 atctactgcc gctgaacaaa cgttcggata atcactaccg gcttgaaaag gcaccgtaca
    16621 ttgtcgtact taaagacacc gaatcccaat atgatcttgc tgaacgcgtt ttcacgcgag
    16681 gtgaaatcac catcaaaaac gtagagttga tttcaatgtt cgcacatgac gcttatatac
    16741 taaaaagatt atgatgctat ccacagccat cgccatattt ttaccaacct tacttggttt
    16801 tctcattatc accataatcc tcagaaacga taaagaaact ttctttggcg aacgcatcgg
    16861 cctttctttc cctcttggcg ccggcatttt gacactgcaa atatttttgt tgggactcat
    16921 gcggattcca ctaacattat ttaattcctt aatgcctgtg tttttcgaac tgatgttact
    16981 ttcattctgg atatggaaaa ataagattgt tttaattccc tcgatttcaa acccaggttt
    17041 gagtttattt ctggagatca agtcccctcg aaaccatggg ttaaagaaat gtttatttgc
    17101 tctgcttatc atatggatca ttgcaaaact tgtttctgta tttattttaa caggactcag
    17161 accgattttt gcctgggatg catgggcaaa ctggtctgcg ggagcaaaaa tcttcttcta
    17221 ctcaaagagc ttgctgcttg atgctcctgc actggaattc tttggcggta atgccgtgga
    17281 tagaatcctg tactatccac tgcacaacac cttactgcag ttatggatga gcctttggat
    17341 gggaaagttt gatgatgtat ttgtgaagtt cttcagcccg gtttattgtc tcagtatgat
    17401 tatctgttta tattacatcg ccattcgtga aatcggcaag ctctatgccc ttgcactgct
    17461 cgccatcttt ctgagttcac cgcttttatc ctatcattcc atcgagatgt atagcgatca
    17521 aatgctcggg gtttatttgt tgttcgcatc cttatctttt ttgaaagcca taaggcaaaa
    17581 cctctctttc tgtatactgg ccggagcata cgcaacgata gctgttttta ctaaaaacga
    17641 ggccttgttc tttgttttgc ccttcctttt atcggccatc gtatatttta ggcatgattt
    17701 aaataaaagc cgcggaaaat atgtcaacct gatttcaatt ttagcacctt ttttagcact
    17761 acttccctgg tttttattta aagtccatta tgggctgggg tttttgggac agttgcgatg
    17821 gttgcgatgg tcacatgaat tatatttcga atattatgcg cattcacctg atacctcatt
    17881 gtggtctttt tttcagccgg acatgatttt agtttatata tacaaaatga tatcgctgga
    17941 caactttaat gtcattttcc ttttctttcc gttaattatt ctggcacatg gaaaaatatc
    18001 aaaagagctt ttgcatcttc ttgttcccat tgcaggttat atgcttttct ttctttttct
    18061 ctacatcttt accgcatatt atatgggaca atcgatgtgt ctcacccgca acatactgac
    18121 attttacccg ctcttctttt ttctaaccat attgcttctt aagaaggatg acactgtact
    18181 ttcttatccg gcacccataa caagataata ccgtgtcgtc attataattc acgggaaatt
    18241 cttccgtata tcaaaatgcg gcattatcag atttactcga taatttattt ttactgagga
    18301 ataaccctat attaaccgcg aaagatcccg caatctgaat caaaaaagga taagacggca
    18361 ttagatggcg attcatatcg tccagaccgg ccagccatat ggaaaaaaat tgccccagca
    18421 cgaacacgat gcaaaacgac tgagcccagg caattttttt gtattttaca gcggcaacca
    18481 tgatcaatac cgattgcagg gcaagcagca gaaacagatt tccgtaggcg gccacgataa
    18541 tatcaaaagc tctttgggcc atgtatttga cataaacaac tgtgcaccgg gcatggctga
    18601 tgaatcgttt gatcctttcg ggagaataat taaccctgtc aggaaagacc gaacagaaaa
    18661 tcatatcctc cgtcggcggc aggtctttcg gaaagttccg ataacagcgc ttgtagttat
    18721 cgataaaatg attattgatc gaaaaaggag cggcataata attgatatgt cgcgttctgt
    18781 cgatgacggc catgaattcc tcatctttcc cgtattcgta agccccgctt atacttaaat
    18841 gagcaatcgt attgttcagc gcgatgttgc tcaaaacaaa ctggccgttg aactttttat
    18901 tcattccgca atatccgagc acacccgcag ccgtgaagca caatcccagc aatccccaat
    18961 agaggatttt tctttcgtcg cgcagtagaa aaaaacggca caacagaaaa agaagcatga
    19021 ccgctatcag gatcaaatac gtcggcttca gcatgatcag gaaaaacggg aaaatgccga
    19081 tcgccagcgc ggcggaccgt ttcggttttt caagaaaccg gacaagcagg gacagcataa
    19141 agaccgatcc ggcaaagcac aggctctccg gattgagatg gacattctga atcaggatgg
    19201 gatgccagca gccgtaaaag agcgttgcaa tcatgcccac ataaacattg ccgatgacgc
    19261 gtttggaaac gaaatagaac gggatgatag acaagaacga cagggcctgc tgaaaaaaaa
    19321 tgagattgcg gaccagatga tctccggaaa catattggaa gaatttgatc acatacggat
    19381 aaagcggcgt gcggtacagg tcaacgcctc ccctgaacaa atccacggcg tagaaatacg
    19441 aaacgctgtc cgaattatac tgcaccattc cgggattcag gaacccgaag taataaattc
    19501 tatacagaag acaaaccaca agaatcacgc ctaaaaaggc gtaatcaggt atgctggcta
    19561 ttttcccttt caaccggtta ttctctgtca tcacgttgtg ttccacgaag ataatcgaat
    19621 atccattttt ttattaccga ttgcaataaa aaaatctctt aatcatccat tatccatccc
    19681 tcgtcgctgc gctcctggga tagttccgtc agcgctttaa tctgcgcttc gcttgatttc
    19741 agcggacgtc actaccattt cccattgaca tcaatcagca agttccctat gctcttctca
    19801 aaaaaacggg gagctcaaaa tggcacgcag actcaacggt aaaaatgcga tcatcaccgg
    19861 agcagccaat ggaatcggtc gcgcctccgc agtgcttttc gcccgtgaag gggccaacct
    19921 tgttcttgcc gatatcagcg aaaccgggct tcggcaggtt gaggacgaaa tcaaagggat
    19981 gggaggaaac gtcgttatcc gcaaaaccga cgttgcatcg gaagaagaaa tccaagcgct
    20041 gatcaatctg gccctggaaa aattcaatac gattgacatc ctggtcaaca acgccggcat
    20101 cggcggcggg ctggacgcgc ttgaagatca gaagcccgag gaatggcatc tcgtttatga
    20161 tatcaatgtc atggggccgg tgtatgccac caagcacatc atcgctcata tgaaagaacg
    20221 tcgccgggga tcgatcatca atatcgcatc agtggccggt ctgctctccg gcgccggcgg
    20281 caatgcctac agcgccagca aggccgcttt aatcaacttc acgaaaacgt ccgcctgcga
    20341 agtcggggcc ttcaacgtcc gcatcaacgc cgtctgcccg ggactcattg aaaccggcat
    20401 gacccagccc tttttcgatt gggcccgcag caccggcaag gaaaatcagc tcggcaaata
    20461 ctgcgagttg aaacgggccg cctctccgga agaagttgcc tgggcgatcc ttttcctggc
    20521 cagcgatgaa gccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    20581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntccggtc
    20641 agcgccgaaa cgagcaacac tccggccctt tccaaaaacc gggccacccc tcaacccgca
    20701 gcgcagaagg caaacaccat cactcaggcc gcattaaaaa acggcgtccg cacctgtgcg
    20761 gggcgaatca atcaggttac caattttctt acggcgggag caaagggcgg cggtttcatc
    20821 atgatatatc cgaccagccg gcctgatcgg cacatgactt ccgtatccat ggaaattcct
    20881 ctgcagaaca acacggccta cgccagcgcc agttttgcgc cggggcaggg aaattactgc
    20941 gaagggctgt atgaaacagt cgtttactgg ccgcagacat gttcggaagt cgcagaaaaa
    21001 aaattcggaa aattcaaaag agctggagcc atatccaaaa atatttatgt ccgggacggc
    21061 ggtgcaacaa aagtttttct gatgccggcg ggcgccggat gcgtatccat caaaaaggag
    21121 ttaatccgat agattaacga ggcaactcgt aaaaaaagat actaaagaat ttttaaaagg
    21181 aggcaataac tcaatgttta agttaatttt aaaaggaggc ttattatgaa aaacagattt
    21241 ttaatgattt tagccttgct ctttgtcgga ctttttttgt ccggcggctt tttagcgggc
    21301 aaggtcatgg cagacgtagt tggagcgtat gtagactcgg gcggtattgc tagcgacata
    21361 gatgagccgc gtggcggata tatgtttgtc gctacgcccg gagatacagg cacaagcacc
    21421 aatgtaagcg gttattacat taacgcctat tttgctgggg aagtcacgca agatccggga
    21481 gccaatttat tttcaataga ttacacgtat gatccagcca cattttctta tacattcggt
    21541 aatgctgcaa cgttaagcct ggacagcggc agcaacgcat atctcagcta ctgggattct
    21601 ataaatagaa tcgaaaacgg actattagcc gatgcaaccg gtgtgagtgt cgggtcaatc
    21661 gactacacct caggcattta tcatggtctg actgttgaca gaacagcatc ttttatcgac
    21721 ggtccctttt tggttgacac caacggcgtt gacactcctg atgttgccgg caccggggtg
    21781 ctgtatcttg acgacggctc tatgctcatc ggcaccacta atctggcaaa taacaactgg
    21841 aacggattaa gtgcagacat gaccagtgca acaatgaccg gtggcaccgg cacgacgacc
    21901 tggacattga gcaacagcgg cgccgtgctt aatgacagca caaacggcga tatttttacg
    21961 gttggcagtt atggcgacac caccgtcgcc ggaacactga ccacaggcga tgtcatctat
    22021 gccggcggaa acttgaatat gggcaatcat gacattaata acgtccgtga tattaacaat
    22081 gtcagagact tctctgtgac acgcaataca acgttgggca atggtagtac cgattccaca
    22141 acggttaccg gtttgtttaa tgtaaacggc gattcttcat tacggtccac cagcggtctt
    22201 ttcggcatgg atataaacga ttctctggcg caactgggct atgacgacgg cgttacaaag
    22261 agaaacggtt ttatggctga cagcggcaga gcggtggcaa attcattaga ttacaccaca
    22321 ggctatcaag caacgttaag tctggacagc accgacaaag catttatcag cttcaacgac
    22381 ggtacggttg aaagcggcct ggcagccgga ccggccggat tgatggtcgg ctcgacaagc
    22441 ggttcgacaa acatgggaat gaccgtcgat acttttacca acgacatgat tgtcggttat
    22501 atggatactt cttcaagcga ggcttatgga cttcatgcca ataactcgga agtcaacctt
    22561 ggttacaata acgcaggaag cttcaggggc gtgaccgtga acaattctct tatatccgca
    22621 tactcacctg atgcaatggg cgccgcgggt atgggtttag gccaaatatc catccctatg
    22681 ggaccttcaa tgtccggcgc atatatcggc tatgataatg gcaccaatta caacggtctc
    22741 gtatcagccg gcataggtac agccgtcggt tcaattaatt atgcgaccgg cgagatttcc
    22801 ggtcttgcca cggacggaac caatttaatg gtcggttatt taaatggaac gaccggcgcg
    22861 accaacggcc tgatggccaa cagtacggaa attgttgtaa actcatttga cagcatgaca
    22921 agcaatgagg caacgttaag cctgaacagc acagacaaag cctacctcag cttctgggat
    22981 gatgcaaata gtatagaaaa cggcttactg gccacgataa gcgatgtcag cgtcggttct
    23041 atgaattata ataccggcac atcttacagt ctcaatgcca acggcagcag tattttcgtc
    23101 ggttcagaca ttggcggcag ccagcatgga ttgaccgttg atgcaacgtc aaccaccctg
    23161 tccggcggca cgacttcttc cgttgcaacg ctggccgacg gcaacacgag ccttaccgtt
    23221 gacggtattg agctatttga tgcgacgaat aatgccggcg ataccacggt cacgatcggc
    23281 aacaccgcca acagtttcat cactgtaagc aacgttgcgg gcgccaccta cggtgaaacc
    23341 atcgcactga acggcgatac gctggttgac ggcgacctga ccgttacagg aacactcaca
    23401 gtcaacggcg ctacgtttac aaacaacggc attaccaatg tcaataatat cgatacagac
    23461 acgctgcaaa cgagcggtct ggcgactctt aactcagcgc aggttgacac aaccctcaat
    23521 gttgacggcg ccacgaccac gcacggcatc accaataccg gcaatattac caactcgggt
    23581 aatatcagca cggcaacgtt gcagaccagc ggtctggcaa cgctcaattc cgctcaggta
    23641 accaacaatc tgacggtaaa cggcaataca acgttgggaa atgcgattac cgatgctaca
    23701 accgtcaacg gaacgctgtc ggtttccggt gcgacaacca cgcatggcat caccaatacc
    23761 ggcaacatta ccaactcggg cgatatcagc accgcaactc tgcaaaccag cggcctggcg
    23821 accctcaatt cggcgcaaat caacacgact ctcaatgtcg acggcgccac gactaccaac
    23881 ggcatcacca ataccggcga cattgcaacg gatactctcc agacatccgg tctggcgacc
    23941 ctcaattcgg cacaggtcaa cacgaccctg aacgtcgacg gagcgaccac gctgggtagc
    24001 acgcttgatg tcgcgggtgc cgcaacactc aacgacacgc tgaacgttgc gggcgatacg
    24061 accctcgcat ccaatctggc tgtcggagga aatacaacac tggccggatc tctggatgtt
    24121 gcaggagcga caaccaccaa cggcattacc aataccggca acataacaac cgatacgctc
    24181 aataccaccg gcgatacgaa tgtcggcggc aatctgtttg tctcgggcgc cacaacctta
    24241 accggcgatc tggttgccaa cgggacgaac aacaccatcg ggaccgcggg tgtttcgacc
    24301 aatacgatca ccggcgttga caataacatc atcgccacgg gcaacaattt cataaccggc
    24361 gacatgaaca ccatcacggc gacaaccggc aacaccatcg gcgttaccgg cgggtcgacc
    24421 atcaccacga cggcggatca ggtaaccgca ctgaccgctg acggcagcgg attcgttgcc
    24481 aataacggcg gcaatgtgac cgtgctgaac acaacgggac acgggttgat cgtttctccg
    24541 acggaaacag ttgtttccgg cggaaccacc tccacaacgc tgacgttgaa caacacggga
    24601 gctaccttca gaaatacggt aaccggcggt ccggcaagag tgtcgggcgt tgccgacggc
    24661 gtcaatgaaa atgatgcggt caacgttcgc cagtacaacg tgctcaagga cggcgtggaa
    24721 aaagccaata tgggtattgc ttccgtatcg gctctttcag ccatccccgc cgcactgccc
    24781 ggaaagcgag tggctctggg cgcaggttac ggttactttg aagatgaaag cgccgtcgcc
    24841 atcggcgtca aggccagagt tctggaaaat atgagcgttt ccgccggcat cggcgtcggg
    24901 ataagcgaca ccagttcgac ttatacggca aatgccggat tctctttcag cttctaatct
    24961 caaagagcaa tctcaaacaa aaagagggga cagcgattat tcgctgtccc cttcttaatt
    25021 gcgtgagccg tttatgtaaa tgtcaaacgg ttgacctgtt tttgccgtaa actatcccag
    25081 tctttttttc agttctgcaa actgtttccg gagtcttgcg ggcaatctgt ctccaaactg
    25141 cgcgaagtgt ttttcgatgt cgggaatttc cgccttccag gcatcgagat cgacgcggag
    25201 caattcctga atatcccgct catcaatttt caggcctttc aaatccagac cgccaagccg
    25261 gggaagcagt ccgatcggag tttgaacggc ttcggccttg ccgtccacgc gctcgcacat
    25321 ccatttcagg acacggctgt tttcaccgaa gcccggccag agccagcggc catccgctgt
    25381 tttcctgaac cagttcacat agaaaatttt cggagccttg tcgccgaggt catcgcccat
    25441 atccagccag tgctggaaat agtcgcccat gttgtagccg caaaacggct tcatcgcgaa
    25501 aggatcgcgc cgcaaattac cgaccgctcc gatgttggct gccgtggttt ccgacgaagc
    25561 cgtcgcgccc agaaagacac cgtgatcgaa actgaaagct tcattcacca gaggcacaac
    25621 gcctgcgcgc cgtccgccga agataaaaat atcaatcggc acgccttccg gtttttccca
    25681 gtccggacaa atcatcgggc actgcgcagc gggggcggta aaccgcgcat tgggatgcgc
    25741 cgccggttca ttcttgtcgt tgggccagtc ccttcctttc cagtcaatgg catgtttggg
    25801 aggcttgccg tccatgcctt cccaccagac gtcgccgttg tccgtcaggg cacagttggt
    25861 aaaaataacg tttttcttga tggtatccat cgccacggga ttggaatcgt aggatgttcc
    25921 cggcgccacg ccgaaga
//
LOCUS       DAOOLB010000026        24485 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_1931_length_24485_cov_42.604380, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000026 DAOOLB010000000
VERSION     DAOOLB010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 24485)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24485)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24485
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1931_length_24485_cov_42.604380"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            161..1291
                     /gene="carA"
                     /locus_tag="PLF77_05085"
     CDS             161..1291
                     /gene="carA"
                     /locus_tag="PLF77_05085"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HPC08263.1"
                     /translation="MFQLGKKRKALLVLEDGSIFAGNAFAGSGETMGEVVFNTGMTGY
                     QEVITDPSYKGQIVTMTYPLIGNYGINDEDRESAGIHLEGFIVKEYQAHPSNWRMTKS
                     LKSFLDDHGKIGIEGIDTRALTRRLRMAGAMKGIISTESANLESLLATLRDYPGLVGR
                     DLVREVTCSTPYQWERNAVRADGIAPDKPQHKLRVAVVDCGVKYNILRLLEKNGCEVV
                     VMPAFSTGDDIMSLKPDGVLFSNGPGDPAALSYIVDTARFIIGKVPVFGICLGHQIIG
                     QAIGGCTEKLKFGHHGINQPVKNAETGRVEITSQNHGFVVVLESMNDQAQKTYHNLND
                     NTSEGMSMPQAFSVQYHPEAAPGPHDTAYLFGRFVEMMKSVK"
     gene            1292..4498
                     /gene="carB"
                     /locus_tag="PLF77_05090"
     CDS             1292..4498
                     /gene="carB"
                     /locus_tag="PLF77_05090"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009455.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HPC08264.1"
                     /translation="MPKRNDIKKILLIGSGPIIISQACEFDYSGTQACKALREEGYEV
                     VLINSNPATIMTDPEMADRTYVEPITPEAVEKIIARELPDALLPTLGGQTGLNTAVTL
                     AESGVLKKYNVEMIGASLDVIHKAEDREKFRHAIEKIGLRMPKSGFARSMQDVLKIAD
                     DIGFPIIVRASFTLGGTGSGVAYNREELMEIAKAGLDASMISEVMIEESVLGWKEYEL
                     EVMRDKADNVVIICSIENFDAMGVHTGESITVAPAQTLTDKEYQNMRDAAIAIMREIG
                     VETGGSNVQFAVHPQTGELIVVEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IPNDITRETMASFEPTIDYCVVKIPRWTFEKFPQAEDFLTTSMKSVGETMAIGRTFKE
                     SLQKAVRSLEIGRFSLEQALPDHIPDKKEFLKSKLIKPNSLRLFYIAAAMQNGLSIEE
                     LYRLTKIDPWFLHQIREIVDAENEIKESTPSVEVLREAKKMGFSDRALASFWKMTEPQ
                     IRQWREKGKIIPVYKLVDTCAAEFEAYTPYYYSTYETENETRLSDKRKVAIIGGGPNR
                     IGQGIEFDYCCVHASFALREEGVESIMINSNPETVSTDYDTSDKLFFEPVTLEDVLHI
                     LQTEKPEGVIVQFGGQTPLNLAKGLETAGINILGTSPDAIDRAENRDRFQEIVTKLKL
                     NQPDNDTARDTQKALQAAERIGYPVLVRPSYVLGGRSMEIVYDEETLKSFMEKALLVS
                     PEHPILIDKFLEDAVEIDVDAVSDGETTVVAGIMEHIEEAGIHSGDSACMLPPLTIPG
                     DVLDLIRKQTTMLAKELNVIGLMNIQYAVKDGKLYVLEVNPRASRTVPFVSKAIGVPL
                     AKLATKVMLGKSLKELGFTESPRPKHISVKEAVFPFNRFPDVDILLGPEMKSTGEVMG
                     IDQSFGLAFAKSQMATGFKIPQQGTVFISVNDQHKDRIVTVAKTFQKIGFKILATKGT
                     ALYLLEHGISAGVVLKLSEGRPNIVDFIKNGDMQIVINTSIGRKSSRDAYHIRHSALI
                     YNILYTTTIAGARALAEATVAIKEKAWQVCSLQEYQKA"
     gene            4654..6075
                     /gene="purF"
                     /locus_tag="PLF77_05095"
     CDS             4654..6075
                     /gene="purF"
                     /locus_tag="PLF77_05095"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HPC08265.1"
                     /translation="MLSEKEQEKRYYPREECGIFATYGHEEASRVAFYGLFALQHRGQ
                     ESAGIVSSDGCNVYEHKGMGLAAEVFKEETLQKLPGKLAIGHVRYSTTGSSNLCNAQP
                     FLVHYGNEYYALAHNGNILNAQKLRRELEDSGSIFQSTMDSEVIIHLMAHYLKQGLEK
                     ALAAALSRLEGAYCLIMLTRDKIIAARDPRGFRPLALGKVSGKWVIASETCAFDLVGA
                     DYVRDVEPGEILIIDATGVKSVNPFSAIRHCHCIFELIYFARPDSQVFRQNVYQYRKR
                     LGHELAREYKPDVDIVMPFPDSGVYAALGYAEESGRPFEMGMIRNHYIGRTFIQPSQA
                     MRDFGVRVKLNPVRALLENKKILIVEDSIIRGTTSRNRVKNLRNIGIKAIHMAVSCPP
                     TVYPCPYGIDFSSKGELVASKMESEKAIADFIGLDSIHYLSVEGMIRATGLKKDCFCL
                     ACFTGHYPIAPPEILDKFCLETK"
     gene            6343..6789
                     /locus_tag="PLF77_05100"
     CDS             6343..6789
                     /locus_tag="PLF77_05100"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPC08266.1"
                     /translation="MGIHTYKAASVLKETGMMVESDARGFKFIADEPRGLGGTNRGMN
                     PMETILCALGACQCMTARFFAMLLKIDLTEYHVDVEGDIDARGFLNADSGIRPGFQEI
                     RLTVRMKANAPEEKIAELLAMVESRCPVGETVLHGAPVVVRHIELL"
     gene            complement(7049..8800)
                     /locus_tag="PLF77_05105"
     CDS             complement(7049..8800)
                     /locus_tag="PLF77_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08267.1"
                     /translation="MAIDYSVIGNKIIMRTGGRLCETPDELLESDLFFRILTECIREL
                     ESRNSLLLNIFGKTTVDQQTIQKMALVLKNLAKIEAELIPKVVEGTEPYFSDINNLYK
                     FVEYLYNFWRSHERYIIIDSFDDSFEKRPYRVFNQRIQGLTELIRDTYRDIQENITGS
                     HPRIYRQLSAGAKISAICTREDIHLSGVYADKLSSVPMIRQVLFYPTLIFDPPMNKRT
                     GQFVRIDTNPMDVVDIRPDEWLCYPAMVGNLLMHVYFHQNFMDLGFSLCNLFELAGDK
                     HLAKKPDAIFLFGVQDGSLDGLAQYPTMFHEDRSNNLLIGACPGSSEFGYFGYLKKMM
                     LTLHNAIMIRQGRLPFHGALYQIHLKNGTEATILMMGDSGAGKSETLEAFRMLGDEHL
                     RGLQIIADDMGSINITEQGDIIGYGTETGAFIRLDDIKPGYAFRNIDRSIIMSPGKTN
                     ARIVLPVSTLEEVNMGVGIEYVLYANNYEEIDDDHPALERFSTPDQAFSVFREGTVMS
                     KGTTTSKGIVHSYFANIFGPPQYREIYDQLARKSFEAFFRKGIFVGQIRTRLGIPGCE
                     MTGPKEAALKLLDMILA"
     gene            9185..10546
                     /locus_tag="PLF77_05110"
     CDS             9185..10546
                     /locus_tag="PLF77_05110"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPC08268.1"
                     /translation="MKEGIVCKFGGTSVANAAQINKVKNIVTMNTKRQYVIVSAPGRD
                     SSDDQKVTDHLLNVATNGRHFRLQNKNITGQQSYEAVLGKFQRLIDELAIDGGDILKN
                     LEKDMKTPLPDVKRLDFMASRGERYNAEVVSRYLKKQGIPSELVLPEEMGLTVTGDFS
                     NARVIPVSYRNIKKKLSSIEGVSVIPGFYGVTEKGDIAVFSRGGSDLTGGEVAFSIEA
                     GLYENWTDTDGIYRADPRLIPEAKVIPRLTYKEIRLLSSKGFDVFHYDAMVNCKKRNI
                     PIQIRNTNNTNSEGTLIVSERVPEEVVVGIARKDNIAFIYIERDGAGETIGFVHDLLS
                     VIKDYGIETYHYPTDRDDMTILINQDDMVGYAEDIKERIEREFKPDTLEFNYNISILS
                     PVGIGMKNHPGVIAEAANALRDKNINIDIIIQGPAQISFHFGFQNFYADSALRAMYNC
                     LIE"
     gene            10893..13481
                     /gene="leuS"
                     /locus_tag="PLF77_05115"
     CDS             10893..13481
                     /gene="leuS"
                     /locus_tag="PLF77_05115"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HPC08269.1"
                     /translation="MKYEPLVIEEKWQKKWEDEKSFKVTEDPQKKKYYLLEMFPYPSG
                     KIHIGHVRNYTIGDVVARYKRMKGFNVLHPIGWDAFGMPAENAAIEHKIHPAKWTNEN
                     IEHMKKQLKRMGFSYDWDREVSTCEPKYYRWEQLFFIWMYEKGLAYKKRSTVNFCTKC
                     DTVLANEQVEGGQCWRCGTEVQEKVLDQWFFKITAYIEELLDYCDKLPGWPERVMTMQ
                     KNWIGKSYGCEVDFPMADGNGAIKVFTTRQDTLFGATFMLVAAEHPLVMELTKGKAIE
                     KDVMQFVEKVKKQDKLMRTSEYYEKEGLFLDSYCLNPLTGGKMPIFAANFVLADYGTG
                     CVMAVPTHDQRDFEFAKKFNLPLIVVISPKDRTLDAATMTEAYVDEGVLVNSGQFNGM
                     ENTKVLEAIADFLEKEGKGKRTIQYRLRDWGISRQRYWGAPIPMINCAKCGIVPVDEK
                     DLPVILPENVKLSTEGGSPLAALKEFVETTCPKCKGPASRETDTMDTFVESSWYFDRY
                     CCPDYDTKPGLDRKKLDYWMPVDQYIGGIEHAILHLLYSRFYTKVLRDFGVIGVDEPF
                     TNLLTQGMVCKETMKCKEHGYLFPDQAADGKCHICGKDVIIGKTEKMSKSLKNVVDPD
                     YLVKKYGADTARIFCLFAAPPEKDLEWSDQGVDGSFRFLGRLWRIVDEYLEDIKGVAP
                     ANGALQLEGDLKSLRRKTHQTIRKVSIDIEDRFHFNTAISAVMELVNVVYQVKRPASS
                     DKVALSVVREALEAAIILLVPIVPHVTEELWQLLGHQNLASDMSWPEFDPAIASEEEM
                     TIVVQINGKLRSRMTVPVDESEEKIKADALADEKIKSMTAGMQIKKVIYVPKKLVNIV
                     VTLSES"
     gene            13670..14203
                     /locus_tag="PLF77_05120"
     CDS             13670..14203
                     /locus_tag="PLF77_05120"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HPC08270.1"
                     /translation="MKNEIGLITKIAIICISCAVLFGCGYSFAPRGESIDTRIQKVYV
                     EPFENNTAQAEIENYVRSAFINQFIQNSRFKLASGTETADAVVRGKIVNLHTSSLTHL
                     KNDLAAEERATMIMEVVFEDKSNGRIIWSAKNITDSVDYVLNEDINMLPASKKQAFMK
                     LADDMAEKAFNLMMSGF"
     assembly_gap    14250..14349
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            14593..16980
                     /locus_tag="PLF77_05125"
     CDS             14593..16980
                     /locus_tag="PLF77_05125"
                     /inference="COORDINATES: protein motif:HMM:NF027594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4922 domain-containing protein"
                     /protein_id="HPC08271.1"
                     /translation="MTPSARQFGTFFLLPFTDVQEALDFISSNKRSVTVIKNLSAYLA
                     RLSCKYLLLVDAEKDISIRESSLRHFLKPFTDQHTGMVYSDFTSKKRNLLKKHPVIDY
                     QAGSIREDFNFGSLLIFSVDAVRTVLQKYGASPSDPDVALYDLRLKTSADYHIRHVRK
                     FLYTVSVKNHKQLAASGGKAETHFAYVAKENFLRQKKLEKIATHHLKRIGAYLPSRTK
                     IVNNEQDGGQWKASIVIPVRNRKKMIADALKSALKQKTDFPFNVIVVDNHSTDGTTDI
                     LRKFASTYPHIHHLIPSRRDLGIGGCWNGAIYSPYCGRYVVQLDSDDLYSSPHTLQKV
                     VNKLRRGRYAMVVGSYTIVDEHLKKIPPGLIAHKEWTQTNGHNNLLRVNGMGAPRAFD
                     RSVICRTGFPNVSYGEDYAVALRITHEYKIGRIYENLYWCRRWKDNTDAGLDAEKKNR
                     NDFYKDQLRTLEIEARKSANKGDACVNSRRVLAEFSGGKALALPFLCQHLYDAQKKTW
                     PKLAEACRNLASVHKRKLPGAYDVYLQYNPARAISSGASVDAQSIRQRPCFLCQENLP
                     VEQKGILYRSQYLILCNPAPIFEKHFTIVSLQHTLQEITSSINGFLQLSADMSGYAVF
                     YNGPACGASAPDHLHFQAVPKKFLPFLREIKKLPVCDTSSLRYVSGETFDRSVVLLES
                     GDQDALSMQFETLLKALQSIMQTKVEPMVNVVCDHAGHRWRLIVFLRQKHRPDAYFEK
                     GEKRIFISPGAVDMAGVVITPLLDNYNRLDYNAVREIYRDVSLSENTTDAVIRKL"
     gene            16995..17549
                     /locus_tag="PLF77_05130"
     CDS             16995..17549
                     /locus_tag="PLF77_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024929.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HPC08272.1"
                     /translation="MFFAEEAIVDSAMSFSEAIEGTTAPDEIILSLSLVDVFYYSFDG
                     RKHQGQMLISKELEDDAYDIFNVIEKIFFPVDKVIPITAYQWNDYRSMADNNTSSFNF
                     RVIEGTTKLSMHSLGKALDINPVQNPVIYPNGVVAPEGAKYQPDERGTFSADHPIVRE
                     FLKRGWHWGGNFNQPKDYHHFEKP"
     gene            complement(17613..18347)
                     /locus_tag="PLF77_05135"
     CDS             complement(17613..18347)
                     /locus_tag="PLF77_05135"
                     /inference="COORDINATES: protein motif:HMM:NF018963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1460 domain-containing protein"
                     /protein_id="HPC08273.1"
                     /translation="MNESTSDQAALGDLIVDIGRLFIGKPYRDGMLENPGKEKLIINV
                     SAFDCTTFVETVLAFFRCAASGRFSSGAFKKYLKLIRYRGGKISGYSSRLHYFTDWMR
                     NNIEKKFLIDITGHLQATPHRKKIDFMSTHPELYPALKNKVQLAKIIDIEKMLSRKAF
                     PVMNKEKLGTRIKKIQSGDVIAFASNQQGLDVAHVGFALWQGNSLRLLHASRQEGKVV
                     ISKKTLLAYLTSNKKFTGIIVARSVF"
     gene            complement(18622..18882)
                     /gene="rpsT"
                     /locus_tag="PLF77_05140"
     CDS             complement(18622..18882)
                     /gene="rpsT"
                     /locus_tag="PLF77_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPC08274.1"
                     /translation="MATHKSAEKRDRQNKKHRQRNIAAKSALKTKIKSVVAGVESKNK
                     ENSAKALKAAIPAIAKAGAKGLIHKKNASRKISRLTKKVNAM"
     gene            19202..20908
                     /locus_tag="PLF77_05145"
     CDS             19202..20908
                     /locus_tag="PLF77_05145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC08275.1"
                     /translation="MTIAVKQQSGKKSINKRKVLQNDLSILARDIIANAGVGMYIVQD
                     GKFVYVSELYQKITGFTEEELIGTYPLDNIHPDDMKTVREKAIKCLKEGNCNPYEYRF
                     ITKSGEIIWVLEAVTSISFKGERAALGSFINITERKLIEKKLHHEEQLFRTLTDQSSD
                     IIAIIDRKGVITYENKAIEPVLGLKVDEQMGGKGFDNLHPDDVPFVSNELKRLFADPD
                     ASASRSEVRLRHKNGQWLVFEFMASGLVNNNVVEAVMINLRDITERKRAEESLRFSEE
                     KYRTILENIQEGYFEVDLAGNLTFYNDPVCEIMGYTREELMSMNYKKYTGNEMAVKVY
                     QAFNKVYLSGNSTKEIDWQIIRKDGARRYIEASVSLQKDFSGHPKGFKGIIRDITERK
                     EMDQKLNHLATHDALTGLPNRILFMDRLQVALVQSRRNRNKLAVMMLDLDHFKDVNDT
                     LGHLAGDKLLQEVGNRLTDILRHNDTVARLGGDEFVVLLSDLERMEYAAGVAKVILKA
                     FGKPFLLADHEITPNVSIGIAFFPDDCDDVESLLQKSDMAMYSVKTHGRNSYKFYSHI
                     AS"
     gene            complement(20922..22532)
                     /locus_tag="PLF77_05150"
     CDS             complement(20922..22532)
                     /locus_tag="PLF77_05150"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPC08276.1"
                     /translation="MKHTKFIFITGGVLSSLGKGLAAASISALLESRGLKVTNQKLDP
                     YINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFCSTCMGKSNNLTTGQVYYAVIT
                     KERRGDYLGKTVQVIPHITNEIKDFIRDTADGFDVAIVEIGGTVGDIESLPFLEAIRQ
                     FRNEVGRENAIFIHLTWVPLIKTAGEVKTKPTQHSVKALREIGIQPDILLCRTENFLS
                     DDIKSKIALFCNVEVHSVFTAKDVSCIYEVPLIFHREGLDAKIVDLLNIWTGQPKLDV
                     WEDVVDKFHNPLHDVTIGIVGKYVNLTDSYKSLNEALVHGGIANNCRVKLKFIDSEKI
                     EAEGLGKHLDDIDAVLVPGGFGIRGIEGMILAIQRAREKKIPFLGICLGMQMAVVEYA
                     RNVCKLEKANSSEFDPETSYPVIDLLPDQRHVKEKGASMRLGGWPCLVEADSFACKAY
                     GQGEISERHRHRYEFNNDYKEALMNQGLRITGISPDGRLAEIVEIKDHPWFLGCQFHP
                     EFKSRPIKPHPLFSKFIEAALNYSRSKT"
     gene            complement(22579..23538)
                     /locus_tag="PLF77_05155"
     CDS             complement(22579..23538)
                     /locus_tag="PLF77_05155"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HPC08277.1"
                     /translation="MGTTIWVTKDFAHMSDVASGIVIKKSIELLKRKKEIALGLATGN
                     SPTGLYKHLAKAVNEGRLDAGRIRSFNLDEYVGLPGNNIQQRVMHPESYAYFMIQELF
                     GHLNKKFIETRVPYGSLIDQKMLIRELKDNKSDWEFKGTDAGKSIAIKAKAQSPYLAW
                     IRKDILDGYVRKIKAAGGIDLQIIGVGGRGHVAFHESGIPFQGSSMLLVKLDDNTIAN
                     AVADGHFKTKADSPQYAISMGAELVYKAKTVVLVANGARKTESVTRSILGNMTPEIPI
                     SYGQAYSQKGGELIYVLDRIAAQGLLENTSALKIRGVILKNLA"
     gene            complement(23557..>24485)
                     /locus_tag="PLF77_05160"
     CDS             complement(23557..>24485)
                     /locus_tag="PLF77_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPC08278.1"
                     /translation="SKGDDIGRAFRLEGVNGRYIVFAKNTFPRNLSLEGMKIVLDTAN
                     GATYRVAPDVFHELGADIDVIHKTPNGLNINDQCGSQHTERLKKTVLENGAAIGLAFD
                     GDGDRLIAVDEKGQEITGDQILVICANVLKREGRLKNDLLVSTVMSNLGLKVACKQYG
                     FKHHASKVGDRYVLEDMLKMGSVIGGEEAGHMIFLDHHTTGDGIIAALQLVAAMIKEN
                     KPLSELARMMDIFPQKLINVDVKSKPDIDQIPRLAEAIKQVEKELGDEGRVLVRYSGT
                     QNMCRVMVEGPTDAVTLKYCRQLADIIKSEIG"
BASE COUNT         6368 a         5637 c         6125 g         6255 t
ORIGIN      
        1 tgcgcgagta tgcggatgcc ctggagatcc tggttgatga cgatatctgg cctgttccga
       61 aattctggga aatgttgttt atctgttgat atggaggcca tcattttttc gccatgtcac
      121 gttttgccgc aaccgatttt atgcaacagg gaagttaacg atgtttcaac tgggtaaaaa
      181 aagaaaagcg ttattggttc tggaagacgg cagtatcttt gcgggaaatg cttttgcagg
      241 gtctggcgaa accatggggg aagtcgtctt caacacgggc atgaccggtt atcaggaagt
      301 gattaccgat ccttcctata aaggccagat tgttaccatg acgtatcccc tgatcgggaa
      361 ctatggcatc aatgatgaag atagggaatc cgccggcatt catctggaag ggtttatcgt
      421 caaggaatat caggctcatc ccagcaactg gcgaatgacc aaaagcctga aatcgtttct
      481 ggacgatcac ggcaaaatcg gtattgaagg tattgatacc cgtgctttga ccagacgtct
      541 gcgcatggcg ggtgccatga agggaatcat ttccacggaa agcgcgaatc tggaatcgct
      601 gctggcgacg ttaagggatt atcccggttt ggttggccgc gatctggtcc gcgaagtgac
      661 ctgttcaacc ccttatcagt gggagcgcaa tgccgtccgg gcagacggca ttgcgcccga
      721 caaaccgcaa cataaactgc gcgtggccgt tgtggattgc ggcgtgaagt ataatatcct
      781 gagacttctg gagaaaaacg gctgtgaggt cgttgtgatg cccgcttttt ccaccggcga
      841 cgacatcatg tcgctcaagc cggacggggt tctgttttcc aacggccccg gcgatccggc
      901 ggctctttct tatattgtgg ataccgcccg tttcatcatc ggcaaggtgc cggttttcgg
      961 catttgtctg gggcatcaga ttatcggtca ggcgatcggc ggctgtacgg aaaaattaaa
     1021 gttcggtcat cacggcatta accagccggt gaaaaatgcc gaaacgggac gggtcgaaat
     1081 cacgtcgcag aatcacggtt ttgtggtggt tttggaatcg atgaacgatc aggctcaaaa
     1141 aacgtatcac aatttgaatg acaacacctc tgaagggatg agcatgccgc aggccttcag
     1201 cgtccagtat catccggagg cggctccggg accgcacgac acggcatatc tgttcgggcg
     1261 gtttgttgaa atgatgaaaa gtgtgaaata agtgccgaaa cgaaacgaca taaagaaaat
     1321 cctgctgatc ggttccggtc ccatcatcat cagccaggcc tgcgagttcg attactccgg
     1381 aacacaggcc tgcaaggcgc tgcgggaaga gggttacgaa gtggtcttaa tcaacagcaa
     1441 tcccgccacc atcatgaccg atccggaaat ggccgatcgc acttacgtcg aacccatcac
     1501 accggaagcc gtggaaaaaa tcattgcccg cgaactgcct gatgcgctgc tgccgacgct
     1561 gggcggacag acaggactca acacagctgt gacgctggct gaaagcggtg tgctcaagaa
     1621 atacaatgtc gagatgatcg gcgcctcgct ggacgtcatt cacaaggcgg aagaccggga
     1681 gaaattccgg cacgccattg aaaaaatcgg tttgcgcatg ccgaaaagcg gttttgcccg
     1741 ttcaatgcaa gatgttctga aaatcgctga cgacatcggg tttccgatta tcgtccgggc
     1801 gtcgtttacc ttgggcggca ccggcagcgg tgtggcctat aaccgggaag aactgatgga
     1861 aattgccaag gcgggacttg atgccagcat gatctccgag gtcatgatcg aagagtcggt
     1921 gctgggctgg aaggaatatg aactggaagt gatgcgcgac aaggctgaca acgtcgtgat
     1981 tatctgctcc attgaaaatt tcgacgcgat gggcgtgcat accggtgaat ctatcacggt
     2041 cgcaccggcg cagacgttga cggacaagga atatcagaac atgcgggacg ccgccattgc
     2101 catcatgcgt gaaatcggcg tagagacggg cgggtccaat gttcagtttg ccgtgcatcc
     2161 gcaaaccgga gaactgattg tcgtggaaat gaatccccgc gtttcacgtt cctcggcgct
     2221 ggcttccaaa gcgacaggat ttcccattgc caagattgcg gcgaaactgg ctgtcggtta
     2281 cacgctggat gaaattccca acgatattac ccgcgagacg atggcgtctt tcgaaccgac
     2341 gattgattac tgtgtcgtaa aaattccccg ctggacattt gaaaaattcc cgcaggccga
     2401 ggattttctg actacatcca tgaaatccgt gggtgagacc atggccattg gccgcacatt
     2461 caaggaatca ctgcaaaagg ccgttcggtc gctggaaatc gggcgttttt ctctcgaaca
     2521 ggctctgcct gaccatatac cggataaaaa agaatttctg aaaagcaaac tgatcaagcc
     2581 caattcgctg cggcttttct acattgccgc ggctatgcaa aacggcctgt cgatcgaaga
     2641 actttatcga ttaaccaaaa tcgatccctg gtttttgcat cagatcaggg aaatcgttga
     2701 tgccgaaaat gaaatcaaag aatcaacccc atcggtggag gtgctgcgcg aggctaaaaa
     2761 aatgggcttt tccgacaggg cgttggcgtc tttttggaaa atgacggagc ctcaaatcag
     2821 acaatggcgc gaaaagggaa aaatcattcc agtgtataaa ctggtcgata cgtgcgcagc
     2881 ggaatttgaa gcctacacgc cttattatta ttccacgtac gagacggaga acgaaacccg
     2941 gctgtcggat aaacgcaaag tagccattat cggcggcgga ccgaatcgaa tcggtcaggg
     3001 cattgaattt gattattgtt gcgttcacgc ctcgtttgcc ttgcgcgagg aaggcgttga
     3061 gagcattatg attaattcca atccggaaac ggtcagcacc gattacgata cgtcggataa
     3121 attgtttttc gaaccggtta cgctggaaga cgttttgcac attttacaga cggaaaaacc
     3181 ggaaggtgtg atcgtgcagt tcggcggcca gacgccgctt aatctggcca aaggcttgga
     3241 aactgccggc ataaatatcc tgggaacatc gcccgatgcc attgaccggg ccgaaaaccg
     3301 cgaccgcttc caggaaattg tgaccaaact caaactgaat cagccggata acgacacggc
     3361 acgagacaca caaaaagcat tgcaggccgc cgagcgtatt ggctatccgg tgctggtgcg
     3421 gccttcctac gtgctgggcg ggcgttccat ggaaatcgtg tacgatgaag aaacgctgaa
     3481 gtccttcatg gaaaaagcgc tgctggtctc gccggaacac cccatcctga tcgacaagtt
     3541 tctggaggac gctgtggaaa ttgacgtgga tgccgtcagc gacggtgaga ccacggtggt
     3601 ggccgggatc atggagcata tcgaggaggc gggcattcat tccggtgaca gcgcctgcat
     3661 gttgcctccc ttgaccattc ccggtgatgt tttggatctg atccggaaac aaaccaccat
     3721 gctggcgaag gaattgaatg tgatcggtct gatgaatatt caatatgccg tcaaagacgg
     3781 gaaactttat gtgttggaag ttaatccccg cgcttcgcga acggtgccgt ttgtcagcaa
     3841 ggccatcggt gtgccgctgg ccaaactggc aacaaaagtc atgctgggca agtctttaaa
     3901 agagctggga tttaccgagt ccccccggcc aaaacatatt tccgttaaag aagctgtctt
     3961 tccgttcaac cggttcccgg atgtggatat tttgctcggg ccggaaatga aatccaccgg
     4021 cgaagtcatg ggcatcgatc aatctttcgg acttgccttt gccaaatcgc aaatggcgac
     4081 cggattcaaa ataccgcagc aaggcactgt cttcatcagc gtcaatgatc aacacaagga
     4141 taggattgta acggtcgcca aaacgtttca gaagatcggt tttaaaatac tggcaacgaa
     4201 aggcactgcc ctgtaccttt tggaacacgg tatcagcgcc ggtgttgttt taaagttaag
     4261 cgagggccga ccgaatatcg tggacttcat caaaaacggg gacatgcaga ttgttattaa
     4321 caccagtata ggacgaaaat cttcgaggga tgcttatcat atcaggcaca gcgcgctgat
     4381 atataatatc ctttatacaa cgaccattgc cggagcgcgc gccctggctg aagcgacagt
     4441 cgcaataaaa gaaaaggcct ggcaggtttg ctccctgcag gaatatcaaa aagcataaaa
     4501 cagcttattt tctcaccttt gaaggttatg gtgtcctata gggtagaaaa gagtgacaat
     4561 taaaaaatag cctctcccct cgatttgtta ccgcggggag aggaaaaaat cagaatgcga
     4621 gagtaatgaa aatccaaaag gttttttgat aaaatgttat cggaaaaaga acaggaaaag
     4681 cgatattatc cccgcgaaga atgcggtatt tttgccactt acggtcatga agaagcatcc
     4741 cgcgtagcgt tttacggtct ttttgccctt cagcatcgcg gccaagaatc cgccggcatc
     4801 gtcagctctg acggctgcaa tgtatacgag cacaaaggca tggggctggc ggccgaggtt
     4861 ttcaaggaag aaacgctcca aaagctgccc ggcaagctgg ccatcggaca tgtccgttac
     4921 tccacgaccg gttcatcgaa tctttgcaat gcccagccgt ttttggtgca ttacggaaac
     4981 gaatattacg ctctggccca taacggcaat attctcaacg cacaaaaatt acgcagggag
     5041 cttgaagaca gcggttccat ctttcaatcc accatggaca gcgaagtgat tatccatctg
     5101 atggcacatt acctgaaaca gggcctcgag aaagcgctgg cggctgcact atcgcgactg
     5161 gaaggcgcct attgcctgat tatgctgacc cgcgacaaaa tcatagccgc ccgtgatccg
     5221 cgcggattta ggccgctggc gctgggcaaa gtgagcggca aatgggtgat cgcctcggaa
     5281 acctgtgcgt tcgacctggt cggcgcagac tatgttcgcg atgtagagcc cggagaaatt
     5341 ttaatcattg atgccacggg cgtcaaaagc gtcaaccctt tttccgcgat caggcactgc
     5401 cattgcattt ttgaactgat ttatttcgcc cgtcccgaca gtcaggtttt tcggcagaat
     5461 gtttatcagt atcgcaagcg gcttggacac gaactggcgc gtgaatacaa accggatgtt
     5521 gatatcgtca tgccgtttcc cgattccggc gtttacgcgg ctttaggtta tgccgaagaa
     5581 agcggccgcc cttttgaaat gggtatgatt cgcaaccact atattggccg cacctttatt
     5641 cagccttcac aggccatgcg cgatttcggc gtccgcgtga aactcaatcc cgtcagagcc
     5701 ttgctggaaa ataaaaaaat cctgatcgta gaggactcca tcatccgcgg cacgaccagc
     5761 cgcaaccggg tcaagaatct gcgcaatatc ggcatcaaag caattcacat ggcggtgagc
     5821 tgtccgccga cggtctatcc ctgtccgtac ggcattgatt tttcatccaa aggagaactt
     5881 gtcgcctcca agatggaaag tgaaaaagcc attgctgatt ttatcggtct ggactccatt
     5941 cattatctgt ccgtcgaagg gatgatcagg gcgaccggcc tgaaaaaaga ctgcttttgc
     6001 ctggcctgct ttaccggtca ctatcccatt gctccgcccg aaatccttga taaattttgt
     6061 ctggaaacaa aatagttttt ccggataaat tcctgcctcg attgtcgtga gcaattgaaa
     6121 ttattgattg ataattggtt gaattgaatt tgaccccaat tccttccaat aaggataatt
     6181 actgattgaa aaaatttccg gagcacatta gattatataa cggtgacgga gcatatcacc
     6241 cgttgaaaga tccggaaaag ttaaccgggc aatcaacgat ttattcatac gtccgttttg
     6301 cagtaatctg tctgaatgga tagttcgtaa acaggagaaa tgatgggaat acatacatat
     6361 aaagcggctt cagttttaaa agaaacggga atgatggtgg aaagcgacgc gaggggattc
     6421 aaatttatcg ccgatgaacc cagggggctc ggcggaacca ataggggaat gaaccccatg
     6481 gaaaccatct tgtgcgccct gggcgcctgc cagtgcatga cggcccgttt ttttgccatg
     6541 ctccttaaaa tcgatcttac cgaataccac gtggatgttg agggagatat cgatgccagg
     6601 ggctttctca atgccgattc gggcatccgt cccggctttc aggaaatccg cttgaccgtc
     6661 cgtatgaagg ccaatgcccc tgaggaaaag atcgctgaac tgctggctat ggtggaaagc
     6721 cgctgccctg tcggtgaaac tgttctccat ggtgcgcctg ttgtcgttcg gcatatcgaa
     6781 ttattatagt tgacgatcaa cgaactgacc tcgggaactt ataaatgaag aatcttgtct
     6841 ttagcatttt cctcaaagaa cgtatccttg tggacgacac cctttagtaa cattttattt
     6901 gagataattc ggtggttgag atttcctcgc atataaagat attttggact gtcccccacg
     6961 gattagacaa aataatggat tgaattgata ttgatggata caaggtattg attggttcaa
     7021 atttcttgtt cttcaggcgt tctgcatact aggccagaat catgtcaaga agtttcagag
     7081 ctgcttcttt cgggccggtc atttcacatc cgggtatacc caggcgtgta cggatttgcc
     7141 cgacgaagat gcccttcctg aagaatgctt cgaaagattt acgcgcgagc tgatcatata
     7201 tttcccgata ctgaggcggg ccgaaaatgt tggcgaaata cgagtggacg atccctttcg
     7261 atgtggtggt ccccttgctc atgaccgtgc cctccctgaa cactgaaaac gcctgatcgg
     7321 gtgttgagaa gcgttcgagt gccggatggt cgtcgtcaat ttcctcatag ttattggcgt
     7381 agagaacgta ttcgatcccg acccccatgt tgacctcttc gagggttgaa accggcaata
     7441 cgatacgtgc gtttgttttc ccggggctca tgatgatcga tcggtcgatg ttcctgaaag
     7501 cgtatccggg cttgatgtcg tcaagcctta taaacgcgcc tgtttcggtc ccatatccga
     7561 ttatatcgcc ttgctccgta atgttgatgg aacccatatc gtctgcgata atctgcagtc
     7621 cacgcaggtg ctcgtccccg agcatcctga acgcctccag tgtttcggat ttgcccgcgc
     7681 ccgaatcgcc catcatgaga atggtcgctt cggtcccgtt cttcaaatga atctgataga
     7741 gcgcgccgtg gaaagggagt ctcccctgcc tgatcatgat cgcgttatgc aatgtaagca
     7801 tcatcttctt gagatagccg aaataaccga actcgctgct tcccggacaa gctccgatca
     7861 acaggttgtt ggaccggtcc tcgtgaaaca tggtcggata ttgtgccagt ccgtcaaggg
     7921 acccatcctg aacgccgaac aggaaaattg catccggttt cttcgcgaga tgcttgtccc
     7981 cggcgagttc gaacaggttg cagagcgaaa accccagatc cataaaattt tggtggaaat
     8041 agacatgcat gagaagattt ccgaccatgg cggggtagca gagccactca tccggccgga
     8101 tatcaactac atccataggg ttcgtgtcga tgcggacgaa ctggccggtc cgcttgttca
     8161 tgggtggatc gaagatcaaa gtcggataga ataatacctg tcgaatcatc ggtaccgatg
     8221 agagcttgtc cgcgtaaacg ccggagagat gaatatcctc tctggtgcag atggcggaaa
     8281 tcttggcccc ggcagacagc tgccggtata tgcggggatg tgatccggta atattttcct
     8341 ggatatcccg atatgtgtct cggattaatt cagtgaggcc ctgaatcctc tggttgaaca
     8401 cccggtaggg tcgtttctcg aaactatcgt caaatgaatc gattataatg tagcgttcat
     8461 gagagcgcca gaaattgtat agatattcca cgaatttgta tagattgttg atgtcggaaa
     8521 aatatggttc ggttccctca actacttttg gaataagttc agcctctatc ttggcaagat
     8581 ttttaagcac cagtgccatt ttttgtatag tctgctgatc aacggtcgtt tttccgaata
     8641 tgttcagaag cagcgaattt cgcgattcga gttccctgat acattctgtt aggattctga
     8701 agaacagatc gctttccagg agttcgtcgg gggtttcgca aagccggccc cctgttcgca
     8761 tgattatttt attgccgatg actgaataat ctatagccat ggttcccctc ctttcgttcg
     8821 tcgtttcgtt gaaaaatatc actgttcccc cgatgaaatc aatccttttc tcaaggggca
     8881 tatgccgcaa gtgatatctt gataacagat tgaacgatgc gtataccgga gtggacgtta
     8941 ttttttcagg cgatattgct atcgaaatta tcatagtcgt ccatctgttg ttccttgcgt
     9001 ttttattgct aaaatttcct ttaaataaag gatatgacaa agagaatgcc ttcccagtgg
     9061 tgttcggctg atagacatcg acgtcaaatt tgtgtataat aaaacacatt ttgagggcgt
     9121 ttgtgcatgt caaaaaaacc caagaaccgg tgtcgctgat aaataaaact tcaaggagaa
     9181 gcgtatgaaa gaagggatcg tatgcaaatt tggcgggaca tctgttgcca atgccgcaca
     9241 gatcaacaag gttaaaaata ttgtcacaat gaacacgaaa cggcaatatg tgattgtttc
     9301 cgcaccgggt agggattctt cggatgatca gaaagtaact gaccacctgt tgaatgttgc
     9361 aacgaatggc agacattttc gcctgcagaa caaaaacatc acgggtcagc aaagttacga
     9421 agctgttctg gggaaatttc agcggctgat tgacgagttg gccattgatg gcggcgatat
     9481 attaaaaaac ctcgaaaaag atatgaaaac acccctcccg gatgttaagc ggcttgactt
     9541 catggcatcc agaggtgaac gctataacgc cgaggttgtg agccgttact tgaagaaaca
     9601 aggcatacct tcagaacttg ttcttcctga ggaaatgggt ttgacggtca cgggcgattt
     9661 ttcaaacgcc cgggtgattc cagtcagtta tcggaatatc aagaaaaaat tgagcagcat
     9721 tgaaggggta tccgtcattc ccggttttta cggggtgacc gaaaaaggcg acattgctgt
     9781 attttcacgc ggcggttccg atctgacagg cggcgaggtc gccttctcca tcgaggcggg
     9841 tctgtacgaa aactggaccg atacggacgg catctatcgg gcggatccgc gcctgattcc
     9901 ggaagcgaag gtgatccccc gccttaccta taaagaaatc cgtctgcttt catcaaaggg
     9961 cttcgatgtg tttcattatg atgccatggt gaattgcaag aaaaggaata ttcctattca
    10021 gatccgaaat accaataaca cgaattctga aggcacgctt attgtatccg aacgggttcc
    10081 tgaagaagtt gtcgtcggta tagcccgtaa agataatatt gcttttattt atatagaacg
    10141 tgacggagca ggagaaacga ttggttttgt gcacgatctt ctgagtgtta tcaaggatta
    10201 tgggattgag acatatcatt acccgacaga ccgggacgat atgaccatct tgatcaatca
    10261 ggatgacatg gtcggttacg cagaggacat caaggaacgg atagaacggg agttcaaacc
    10321 cgataccctt gaattcaatt ataacatctc tattctttct cccgtaggca taggcatgaa
    10381 aaatcatccc ggcgttatcg cggaagcggc gaatgcattg cgtgataaga acatcaacat
    10441 cgatatcatc atccagggac ctgcgcagat aagctttcac ttcggatttc agaacttcta
    10501 tgctgattcc gccctgcgcg ccatgtataa ttgtctgatc gaataaagcg tcagtgatga
    10561 tagctgatga agtgcaagct tttggggccg gcgatgaccg gcgttgccgt ttcagtcgca
    10621 aaagtttcaa agtaatcctc cacgcaatga taataatccg aatcctgcgg tcggcgctgg
    10681 tatgcggcta cgggcaatgg catataatcc tcttccataa aacggataaa tgcattccga
    10741 agccacaaga cttaatagga caaaagtcct attgttaaga agaaaataat gatgtgtcaa
    10801 atcgtacagt gttttcgata tagccattgc gctcatcgtg tctatatgtt aagcaaatca
    10861 gcaacgttca ccatccggag agattttgaa aaatgaagta cgaaccgtta gttatcgaag
    10921 agaaatggca aaagaaatgg gaggatgaaa aatccttcaa ggttaccgaa gatccgcaaa
    10981 agaaaaaata ttatctgctg gagatgttcc cttatccctc cggcaaaatt catatcggtc
    11041 atgtccgcaa ttacaccatc ggcgatgttg ttgcccgcta caaacgcatg aaaggtttca
    11101 atgttctgca tccgatcggc tgggatgcct tcggcatgcc tgcggaaaac gccgccatcg
    11161 agcataagat tcatcccgcg aaatggacca acgaaaatat cgagcacatg aaaaaacagc
    11221 tcaaacgcat gggcttcagc tacgactggg acagggaagt ttccacttgc gagccgaaat
    11281 attaccggtg ggaacagctt ttctttatct ggatgtatga gaaaggcctg gcttataaaa
    11341 aacgttccac cgttaatttc tgcaccaagt gcgatacggt gctggctaac gaacaggtcg
    11401 aaggcgggca gtgctggcgc tgcggcacgg aagtgcagga gaaggtgctg gatcagtggt
    11461 ttttcaaaat caccgcatac attgaggaac ttctggatta ctgtgacaag ctgcccggct
    11521 ggccggaacg cgtcatgacg atgcagaaaa actggattgg caagagctat ggctgcgaag
    11581 tggacttccc gatggccgac ggcaacggcg ccatcaaagt attcacaacc cgccaggaca
    11641 cgctcttcgg cgcgaccttc atgctggtag ccgcggaaca tccgctggtt atggaactga
    11701 ccaaaggcaa ggcaatagaa aaagatgtca tgcagtttgt cgaaaaagtc aaaaagcagg
    11761 acaaactgat gcgcacttct gaatattacg aaaaagaagg tctcttcctt gattcgtact
    11821 gtctcaatcc tctcaccggt gggaaaatgc cgatcttcgc ggctaatttt gttctggccg
    11881 attacggcac cggctgtgtc atggccgtgc ccacgcacga tcagcgcgac ttcgagtttg
    11941 ccaaaaaatt caacctgcct ttgattgtgg tcatttcgcc gaaagacaga acgctcgatg
    12001 cggcgacgat gactgaagcc tacgttgatg aaggcgtttt ggtgaactcc ggccagttca
    12061 acggtatgga aaacaccaag gttctggaag cgattgccga ttttctggaa aaggaaggga
    12121 agggcaaacg caccattcag taccgtctgc gcgactgggg catttcccgc cagcgttact
    12181 ggggcgcgcc gattccgatg attaactgtg ctaaatgcgg catcgttcct gttgatgaaa
    12241 aagacctgcc ggttatactg cctgagaatg ttaagttaag caccgaaggc ggttcgccgc
    12301 tcgcggcgct gaaagaattt gtggagacaa cctgtccgaa atgcaaaggt ccggcctcgc
    12361 gtgagacgga cacaatggat acattcgtgg agtcgtcctg gtatttcgac cgttactgct
    12421 gcccggatta cgacactaaa cccggtcttg accgcaaaaa actggattac tggatgcccg
    12481 tggatcagta catcggaggc atcgagcacg cgattctgca tctgctttac tcgcgctttt
    12541 ataccaaggt attgcgcgat ttcggcgtga tcggtgtgga cgaaccgttc accaatctgc
    12601 tgacgcaggg catggtctgc aaggaaacaa tgaaatgcaa ggagcacggt tatcttttcc
    12661 ccgatcaagc ggcagatggt aaatgccaca tctgcgggaa agacgttatt atcggcaaga
    12721 cggagaaaat gtccaaatcg ttgaaaaacg ttgttgaccc cgattatctg gtcaagaagt
    12781 acggtgccga tacggcgcgg atattctgcc tgtttgccgc gccgccggag aaggatctgg
    12841 aatggagcga tcagggggtg gacggctctt tccgttttct gggacgactg tggcgtattg
    12901 ttgatgaata tctggaagac atcaaaggcg tggctcccgc aaacggcgcg ttgcaactgg
    12961 aaggcgattt aaagtctctg cgtcgaaaaa cccatcagac catccgcaag gtgtccattg
    13021 atattgaaga ccgcttccat tttaacacgg ctatcagcgc cgtgatggaa ctggtgaatg
    13081 ttgtttatca ggtcaaacgt cccgcaagtt ccgataaggt tgctttatct gtggttcgcg
    13141 aggcgttgga ggcggccatt attttgctgg ttccgattgt ccctcatgtt acggaagaac
    13201 tctggcagtt gctcggacat caaaatctgg catcagacat gtcctggccc gagtttgatc
    13261 cggctattgc cagcgaagag gaaatgacga ttgtcgttca gattaatgga aagctcagaa
    13321 gccggatgac cgtgccggtg gatgagagcg aagaaaaaat caaagcggat gcactggcag
    13381 atgaaaaaat caagtctatg accgccggca tgcaaatcaa gaaagtaatt tacgtgccga
    13441 aaaagctggt taatattgta gtgacgctat ctgagagcta gcggagcgcc gctcgtccct
    13501 gccacctgaa ggtggcggga taattcgttt taagactata gtcaaggttc attaaagagt
    13561 aagttaaact attcccgaag cgaagcagga ttaagattta tcgcggtcaa atgaaattag
    13621 caaagatatt tcgacacagt tcaaaagctt tgtaattaaa ggtattagga tgaaaaacga
    13681 aattggttta ataactaaaa tcgcaataat ttgtatttcc tgcgccgttt tattcggctg
    13741 cggttattcc tttgctccgc ggggagaatc catcgatacc cgcattcaaa aagtttacgt
    13801 ggagcccttt gaaaataaca ctgcgcaggc tgaaatagaa aattatgtcc ggtcggcttt
    13861 tattaatcag ttcatacaaa acagccgttt taaactggct tccggcaccg agacggctga
    13921 tgcggtcgtc agaggaaaaa ttgtcaatct tcacacctca tcgcttacgc atttgaaaaa
    13981 cgatttggcg gcagaagagc gggcgactat gatcatggaa gtggtctttg aagacaaatc
    14041 caacggcaga attatctgga gtgcgaaaaa tataacggac tcggttgact acgttttaaa
    14101 tgaagatatt aacatgctgc ctgcgtcaaa aaaacaggct tttatgaaac tggccgacga
    14161 catggcggaa aaagcattca atctgatgat gtccggattt tagtgagcca agccgaagac
    14221 gaacgaagtg aagtatgagg cgtaaggcgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14341 nnnnnnnnna actatccttg aagcgcagcg gagcgggatt taagccaagc cgaagacgaa
    14401 cgaagtgaag tatgaggcgt aaggcgaact atccttgaag cgcagcggag cgggatttaa
    14461 gccaagccga agacgaacga aatgaagtat ggggcgtaag gcgaactatc cccgaagcgc
    14521 agcggcgagg gataatgagc acatttttat tatcccgata gtggagcata tcgggataag
    14581 gtacgattta atatgacacc ttcagccaga caattcggca cgttctttct tctccccttt
    14641 actgacgtgc aagaggctct tgattttatt tcctccaata agcgttccgt cacagtcata
    14701 aaaaaccttt ccgcctatct tgcaaggtta tcctgcaaat atctgctgct ggtcgatgcc
    14761 gaaaaagaca tcagcattcg ggaatcgtct ttgcgacatt ttctaaaacc atttacggat
    14821 cagcacaccg gcatggttta ttctgatttc acctcgaaaa aacgaaatct tctgaaaaaa
    14881 catcccgtaa ttgattacca ggccggcagc atccgtgagg attttaactt cggctctctt
    14941 ttgatttttt ccgttgacgc tgtccgaacg gtattgcaaa aatacggtgc ttcaccgtcc
    15001 gatccggatg tggcgttgta cgacctgcgt cttaaaacgt ctgcagacta tcacatccgt
    15061 catgtgcgta aattcttgta cacggtttcc gttaaaaacc ataaacagct cgcagcctcc
    15121 ggcgggaaag cggaaaccca ctttgcttat gtggcaaaag aaaatttcct gcgccagaag
    15181 aaactggaaa aaattgccac ccatcacctg aaacgcatcg gggcatatct gccttcgcgt
    15241 acaaaaattg tgaataacga acaggatggc ggccagtgga aagccagtat cgttattccc
    15301 gtccgtaacc ggaaaaagat gattgccgat gcgctgaaaa gcgccctgaa acagaaaacc
    15361 gatttcccgt tcaatgtcat tgtagtggat aaccactcca ccgacggaac cacggatatt
    15421 ctgcggaaat ttgcatcaac ttatccccac attcatcatc tgattccttc gcggcgcgat
    15481 ctcggaatcg gcggttgctg gaatggagcc atttattcgc cgtattgcgg ccgttatgtg
    15541 gttcaactgg actcggatga cctttacagc tcgccgcata cgcttcagaa agtcgttaat
    15601 aaattgagac gcggacgata cgcgatggtg gtcggttctt acaccattgt tgacgaacat
    15661 ttgaaaaaga ttccgccggg actgatcgcg cataaagaat ggacgcagac gaacgggcat
    15721 aataatttgc tgcgcgtcaa cggcatggga gcgccccgtg cgtttgaccg gtctgttatt
    15781 tgccggacgg gtttccccaa tgtcagctac ggggaggatt atgcggtggc attgagaatc
    15841 acccatgaat ataaaatcgg caggatctat gaaaatttgt actggtgccg acgatggaag
    15901 gataacacgg atgccggtct tgacgcggag aaaaaaaacc gcaacgattt ttataaggat
    15961 caactgcgaa cactggaaat agaggccaga aagtcggcca acaaaggaga cgcgtgtgta
    16021 aacagccgga gagttttagc tgaattttcg gggggcaagg ctttagcatt gccttttctt
    16081 tgtcaacatc tttatgatgc gcagaagaaa acctggccga aactcgctga agcttgtcgg
    16141 aatttggcgt ccgttcataa aagaaagctt cccggcgcct atgacgttta tctgcaatat
    16201 aatcccgccc gcgcgatcag cagcggcgca tctgttgatg cccagtccat cagacaaagg
    16261 ccctgcttct tatgtcagga aaatcttccg gtggaacaga aagggatttt gtacagaagc
    16321 cagtatctga ttctctgcaa tcctgcgccg atctttgaga agcattttac aatcgtatcc
    16381 ttgcaacata cgttgcagga aattacgtcg tcaataaacg ggtttttgca attgagcgcc
    16441 gatatgtccg gttacgctgt tttttacaac ggtccggcct gcggcgcttc tgctcccgac
    16501 catttgcatt ttcaggcggt tcccaaaaaa tttcttccct tcctccggga gattaaaaaa
    16561 ctgcctgttt gcgatacatc gtctttgcga tatgtgtcgg gcgaaacgtt cgatcgatcc
    16621 gttgttttgc tggaatccgg ggatcaagac gctttgagca tgcaattcga gacgttatta
    16681 aaggcgctgc aaagcatcat gcagacgaaa gtcgagccga tggtcaacgt tgtttgtgat
    16741 catgcgggac atcgctggcg tctgattgtt tttctgcgtc aaaaacatcg gccggatgct
    16801 tattttgaga agggggaaaa gcgaattttt atttcgccgg gagccgtgga catggccggc
    16861 gtcgtgataa cgccactgct ggataattat aaccgtctgg attataatgc tgtccgtgaa
    16921 atttaccgtg acgtatcgct ctctgaaaac acaacggacg cggtcattag aaaattataa
    16981 aaagaaggtt gcgaatgttt tttgccgaag aagcgattgt ggacagcgcg atgagttttt
    17041 ccgaagcgat tgaagggaca accgctcccg acgaaatcat tctttcattg tcgcttgtgg
    17101 atgtgtttta ttattctttt gacgggagaa agcatcaggg acagatgctc atcagcaaag
    17161 aattggaaga cgatgcttat gacatcttca acgtcatcga aaaaattttc ttcccggtag
    17221 acaaggtgat tcccataacg gcctatcaat ggaatgatta ccggtccatg gcggacaaca
    17281 atacgtcctc tttcaatttc cgggtgattg aaggaaccac taaattatcc atgcattctc
    17341 tgggtaaggc tctggatatc aacccggtgc agaatccggt gatttatccc aacggcgtgg
    17401 tggcgccgga aggagcgaaa taccagccgg atgaaagggg aaccttttca gcggatcacc
    17461 cgattgttcg ggaattttta aagcgcggct ggcactgggg tgggaatttt aatcagccga
    17521 aagactatca tcacttcgaa aaaccatgat gccgacgtca aatcaaaata ctatcagctc
    17581 cgttctgatg tcttctttga cttgaaatcg atctagaata ctgatcgggc gacaataatg
    17641 ccggtgaatt ttttgttgga tgtcagataa gccaacagcg tttttttgga aatcaccact
    17701 ttaccttcct gacgggaagc atgaagcaga cgtaaactgt ttccttgcca cagagcaaaa
    17761 ccgacatgcg ccacatcaag cccttgctga tttgaggcaa aggcgatcac atcgccgctt
    17821 tggatttttt ttatccgcgt tccgagcttc tctttattca ttacaggaaa agccttccgg
    17881 gaaagcatct tttcgatatc gattattttg gccagttgta ctttgttttt cagagccgga
    17941 tataattcgg gatgcgtgct catgaaatcg atttttttac ggtgcggggt tgcttgcaga
    18001 tggccggtta tatcgatgag aaatttcttt tcaatattgt tgcgcatcca gtcggtaaaa
    18061 taatgcaggc gggacgaata tccgctgatt tttcccccgc ggtaacgaat caatttaaga
    18121 tattttttaa aagctccgga tgaaaatctg ccggaagccg cgcaccggaa aaaagctaaa
    18181 acggtttcaa caaatgtcgt acaatcgaaa gctgatacgt taatgattaa tttttccttg
    18241 ccgggatttt caagcattcc atcccggtag ggcttgccga taaaaagccg tcctatgtcc
    18301 actattaaat cgcctaacgc cgcttgatcg gatgtacttt cgttcatgga cattcctttt
    18361 atttatttct aaatgattat cgaggacaaa gcaaatataa atatttgaga agaggagaaa
    18421 atgaatgcct gaaaaggaaa ggccggtttt ataaccggcc tttccttaat ttgtattgtt
    18481 gatcatcttc tgctacggaa tttctttcaa cttcgaccca ttcggattga atagttcaac
    18541 ggaccaattt taatgcgcct atagtgaccc catggtgttc ttcaggtgtc catgttatac
    18601 gcttttaaaa ttggagccaa actacatggc gttgactttt ttggtcagac gggaaatttt
    18661 gcgggaggcg tttttcttat gaatcaagcc ctttgctccc gctttggcaa tagcgggaat
    18721 ggcagccttc aaggcttttg cggaattttc cttatttttg ctttcaacgc ccgcaacgac
    18781 cgatttgatc tttgttttta aagccgactt ggcggcaata ttgcgttgac gatgtttttt
    18841 attttgccgg tcgcgttttt ctgctgattt atgtgttgcc aaaatatcct cctgaattca
    18901 tttataggtg cggttatcta gcccaattat tgaagactgt caagtataaa ctgagtcatt
    18961 atagcttggt atcctttctc cctatcacaa aaaacactat cattttcgat gggatggcat
    19021 atttaaggtt taaaaactta taaaaatttt tcttgacata atatcctttc ttcaatatgc
    19081 aactcggtta tacactcaca ggaaactatg gtttcatcta ttttctgcga cgcaatattc
    19141 aaagattagt tgaatggaca atgcattttt aatgtatttg ccgttaattt aatgtggtta
    19201 tgtgactata gccgtgaaac agcagtcggg aaaaaaatcc attaataaga gaaaagttct
    19261 ccagaacgac ctgtctattc tggccagaga tatcattgca aacgccggcg tcggaatgta
    19321 tatcgtccag gacggcaaat ttgtttatgt cagcgaatta tatcagaaaa ttaccggctt
    19381 tacggaagaa gaactaatcg gtacatatcc gctggataat attcatccgg acgatatgaa
    19441 aaccgtcaga gaaaaggcga taaaatgttt gaaggaaggg aactgcaacc cttacgaata
    19501 caggttcatt acaaaaagcg gtgaaataat ctgggtgctg gaagcggtca cctccattag
    19561 ctttaaaggg gagcgggctg ccctgggcag ttttataaac atcaccgaac gcaagctcat
    19621 cgagaaaaaa ttgcaccatg aggagcaact gttcaggaca cttacggatc aatcttcgga
    19681 tatcatcgcg attattgacc ggaaaggtgt tattacttat gaaaataagg ccattgaacc
    19741 ggttctgggt ttgaaagtcg atgaacagat gggcggcaag ggctttgaca atcttcatcc
    19801 ggatgatgtg ccgttcgtct ccaatgaatt aaaaagactg tttgcggatc cggatgcttc
    19861 tgcttcgcgt tcggaagttc gtctccgcca caaaaacggt cagtggctcg tgtttgaatt
    19921 tatggccagc ggtttggtga acaataatgt tgtggaagcg gtgatgatca acctgcggga
    19981 tattaccgaa cggaaaaggg ctgaggagtc gcttcggttc agcgaggaaa aatacagaac
    20041 cattctggaa aacattcagg aaggatattt tgaggtcgat ctggccggta atctgacttt
    20101 ttataacgat ccggtttgcg aaatcatggg ctataccaga gaagaattga tgtccatgaa
    20161 ttataaaaag tacaccggta acgaaatggc ggtgaaggtt tatcaggcat tcaataaagt
    20221 ttacctgagt ggcaattcca ccaaggaaat tgactggcag attatcagaa aagacggagc
    20281 caggcgctat attgaagctt cagtatcgct gcaaaaggat ttttccggcc atccgaaagg
    20341 attcaagggc atcatccgtg atattaccga gcgcaaggaa atggatcaga aattgaatca
    20401 tctggccaca cacgatgcac tgaccggtct gcccaatcga atcctgttta tggatcgtct
    20461 tcaggttgcg ctggtgcaat ccagaagaaa ccggaacaaa ctggccgtca tgatgcttga
    20521 cctggatcat ttcaaggatg tgaatgacac gctgggacat ctggcgggcg acaaactgct
    20581 tcaggaggtg ggcaatcgcc tgacggatat tttacggcat aatgacaccg ttgcgcggct
    20641 gggcggtgac gaatttgtgg ttttgttgtc ggatcttgaa agaatggaat acgcagccgg
    20701 cgtggccaaa gtcattttga aagcttttgg gaaacctttt ttactggccg atcatgaaat
    20761 tacacccaac gtcagtatcg gcattgcctt cttccctgat gattgtgatg atgtcgaatc
    20821 gcttttgcaa aaatcggata tggccatgta ttcggttaag acgcacggac gtaacagcta
    20881 taaattctac agccacatcg cgtcttgaaa accaaacatt gttacgtttt ggagcgggaa
    20941 tagttcagcg cggcttcgat aaatttgctg aaaagcgggt gaggtttgat cggtctggat
    21001 ttgaattcgg gatggaactg gcatcccaga aaccagggat gatctttgat ttccacaatt
    21061 tccgccaaac gtccgtcggg tgaaatccct gttatgcgca atccctgatt catgagcgct
    21121 tctttgtaat cgttattgaa ttcgtaacga tgacggtgcc gctccgaaat ttcaccctga
    21181 ccgtaagctt tgcaggcgaa ggagtccgcc tcgacaaggc agggccaccc gcccaatctc
    21241 atggaggcgc ccttttcttt gacatggcgt tggtcgggga gcagatcaat gaccggatag
    21301 gacgtctccg gatcgaattc ggaactgttt gctttctcca gtttgcaaac gtttctggca
    21361 tattcgacga cagccatttg catgcccaga caaatgccga ggaagggaat ctttttttct
    21421 ctggcgcgct gaatggcgag aatcatgccc tcaataccgc ggataccgaa gccgccggga
    21481 accagaacgg cgtcgatatc gtccagatgt ttgcccaggc cttccgcttc aattttttcc
    21541 gaatcgataa atttcagttt cacgcggcag ttgttggcaa tgcccccatg cacgagcgcc
    21601 tcgttgaggc ttttgtagga atccgtcaga ttcacgtatt tgccgacgat gccgatcgtc
    21661 acgtcatgca aagggttgtg gaacttatcg accacatcct cccaaacgtc gagtttcggc
    21721 tgtcctgtcc agatgttgag aagatcgaca atcttcgcat ccagtccctc ccggtgaaaa
    21781 atcagcggca cttcgtagat gcagctgaca tccttggcgg taaagacaga atggacctcc
    21841 acgttacaga aaagagcaat cttggatttg atatcatcgg aaaggaaatt ttccgtccgg
    21901 caaagcagga tatccggctg gatgcctatt tctctgagcg ccttgacgct gtgctgagtg
    21961 ggcttcgttt ttacttcacc ggctgtcttg ataaggggca cccaggttaa atgaatgaaa
    22021 atggcatttt ctctgccgac ttcattgcgg aactgccgga tggcttccag gaaggggagg
    22081 ctttcaatat ccccgacggt gccgccgatt tccacaatgg ccacatcaaa accgtctgct
    22141 gtgtcgcgga tgaaatcttt tatttcgttg gtaatatggg gaatgacctg aacggttttc
    22201 cccagataat ctccgcgccg ttctttagtg atgacggcgt agtaaacctg tcctgtggtc
    22261 aggttgttgc ttttgcccat gcaggtggaa cagaatcgct cgtaatgtcc cagatcgaga
    22321 tcggtttccg cgccgtcatc ggttacgaac acttcgccgt gctgaaaagg actcatggtt
    22381 ccgggatcga cattaatata agggtccagt ttttgattgg tgactttcag accccggctc
    22441 tccagcagag cggagataga cgcggctgcc agaccttttc ccagtgaaga aagaacgccg
    22501 ccagtgatga agatgaattt tgtatgtttc acaatgcccc ttcctgatgt gtttatgcga
    22561 aggttaaatg tccgatggct aagccaagtt ttttaaaata acccctctta ttttaagggc
    22621 gcttgtattt tccaaaaggc cctgggcagc gatacgatca agaacataga tcaactcgcc
    22681 gcctttttgg gaataagcct gaccgtacga tatggggatt tctggcgtca tgtttcccag
    22741 gattgaccgc gtaacggatt ccgttttgcg tgcgccgttg gcgaccagaa cgacggtttt
    22801 tgccttgtaa accagttcag cccccatgct gattgcatat tgcggcgaat ccgccttggt
    22861 tttaaaatgc ccgtcggcca ccgcgttggc gatggtgttg tcgtccagtt tgaccagcag
    22921 catggaactg ccttgaaaag gaatgcccga ttcgtgaaag gccacatggc cgcgaccgcc
    22981 cacaccgata atctgcagat cgattcctcc ggctgctttt atttttctga cgtatccgtc
    23041 gagaatgtct ttccggatcc aggcaagata aggtgattgc gcttttgcct tgatcgcaat
    23101 ggatttcccg gcgtccgttc ccttgaattc ccagtcgctt ttgttgtcct tcaattcccg
    23161 gatgagcatt ttctggtcga tcagggaacc gtaaggaaca cgggtttcta taaacttttt
    23221 attgagatga ccgaaaagtt cctgaatcat aaagtaggca tagctttccg gatgcataac
    23281 gcgctgctga atattgttcc ccggtaagcc gacatattca tcgagattaa aactgcgaat
    23341 gcggccggca tccaatcggc cttcattgac cgctttggcc aaatgcttat aaaggccggt
    23401 cggtgaattt cccgtggcca atcccagagc gatctctttt ttccttttaa gcagttcgat
    23461 ggatttttta ataacgatgc ctgaagcaac gtcactcatg tgcgcgaagt ctttggttac
    23521 ccaaatcgtt gttcccattc tgtttatcct tcccccttat ccgatttcgc ttttgatgat
    23581 atccgcaagc tgccggcaat atttcagcgt gacggcatcg gtcggacctt cgaccatcac
    23641 gcggcacatg ttctgcgttc cggaatacct gaccaggaca cgcccttcgt cacccagctc
    23701 tttttccacc tgtttgatgg cttccgccag cctgggaatt tgatcgatat ccggcttgct
    23761 tttaacatca acattgatca acttttgcgg aaaaatgtcc atcatgcgcg ccagttcgga
    23821 aagaggcttg ttttctttaa tcatggcggc tactagctgc agggctgcaa taattccgtc
    23881 gcctgtggtg tgatgatcca gaaaaatcat gtgtccggct tcttctccgc cgattacgga
    23941 tcccatcttc agcatatctt caaggacata acggtctccc actttagacg catgatgctt
    24001 gaatccgtat tgcttgcagg cgaccttcag acccagattg ctcatgacgg tgctgaccag
    24061 aagatcgttt tttaatcgtc cttcccgctt taagacgttg gcgcagatga ccagaatctg
    24121 atctcccgtt atttcctggc ctttttcatc cacggcaatc aggcgatcgc cgtccccgtc
    24181 aaacgccaag ccgatggccg ctccgttttc cagcacggtc tttttcagac gttccgtgtg
    24241 ctgggaaccg cactggtcgt tgatattcaa accgtttggc gtcttgtgaa tgacgtcgat
    24301 atccgcgccc agttcgtgga acacatccgg cgcgacccga taggttgctc cgttggcggt
    24361 gtccagaaca attttcatgc cttccaaaga cagattgcgg ggaaatgtgt tttttgcaaa
    24421 aacgatgtac cgtccgttga cgccttccag gcggaaggcc cggccgatat cgtcgccttt
    24481 ggaaa
//
LOCUS       DAOOLB010000027        24065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_1976_length_24065_cov_46.859975, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000027 DAOOLB010000000
VERSION     DAOOLB010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 24065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24065
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1976_length_24065_cov_46.859975"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(9..629)
                     /locus_tag="PLF77_05165"
     CDS             complement(9..629)
                     /locus_tag="PLF77_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPC08279.1"
                     /translation="MKKIVQYAAMITLAVLFAVYPFQPASAQVGLYLGGWGGYTIHPN
                     ATEDDWDYYDDVDLDFDETSAWGAKIGYTNPFFKYLALEAEYLYLNPEVEDDYVLDGD
                     VKMSNLMFNIIGKYPLGIIQPYFGLGLGFSYADTSWDGIRGIDDGDYTSYAWQIMTGL
                     EIFLTSEIAVDVGYRYFATEMEFDHEDSDDVTFSTGMVTLGLKVFF"
     gene            complement(717..1286)
                     /locus_tag="PLF77_05170"
     CDS             complement(717..1286)
                     /locus_tag="PLF77_05170"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HPC08280.1"
                     /translation="MKVLGIMGSPRIGGNSDILLDDALAGAEEAGARVEKIVLVKKKI
                     SGCLDCKKCNDTGLCVVKDDMPAIHKKILEADAIIHSVPVYFWLMTAQMKAYLDRWCA
                     LFDATWSWQKSYYPKMKGKRIGLITVCGDPNVHTADPIVHSFKSTSDMTKMRWMGAVM
                     ASANDKGEIVKDEKARKKAFKLGKKAATP"
     gene            1420..2235
                     /locus_tag="PLF77_05175"
     CDS             1420..2235
                     /locus_tag="PLF77_05175"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HPC08281.1"
                     /translation="MPESFHFFSYHRREQDDFTVIAHRGASAYYPENTMPSFEGAIVM
                     GADMAELDVQLTADQEVVVFHDEKISRCTNGRGRIADYDLAYLKQLDAGKWFDKKFKN
                     TRIPELADVLALCKNRIAVNIEIKTEAVDDIIAGGIEEKCLQLVEQYGMKKHVVFSSF
                     DPRAILHLKQIDKTAPVAVLFEKKLYGSRLPSDIVKSLHADAFNCSASELNKKWLADL
                     HSNNIPVNIYTVNNEKKMRQYLKLGVSGIFTNKPDVLKNVLADGDQKSKKTNL"
     gene            2232..4412
                     /locus_tag="PLF77_05180"
     CDS             2232..4412
                     /locus_tag="PLF77_05180"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPC08282.1"
                     /translation="MNISKDLVNFIMFSLDTLIQTAGIDIRLHGLEKVPDQPVVYVIN
                     HFTRLETVLMPYLIKKHLHKEAVSLAHESFFSGKMKDFMDNVGAVSTADPHKDKIFIH
                     ALLSDRHPVIIFPEGQIIKDKKIVEKGKYIVYNTGIRRPPHSGAARIALLTEFIREKL
                     RTLKARNDMASIARIASRFDFSGNDLEKIIRKETYIVPVNITYYPVRARENALSRLAG
                     KFFKNMSARLQEELEVEGPIVLGKVDIDINFGRPIAIKRYIHSKWKMKRMMADGNVYL
                     NTGDFKRISAFRQVCVRLMYDYMNAIYGMTTVNHDHIMSYILTSYPQGSFSENDFKKR
                     IFLAIEHLRKIALPNCHTSLYKKQFYLLTDDQHEKYESFMNEAVTNNYLRRENGVITK
                     NKERFSVPSDFHTIRRDNIIEVLRNEIKPLRHFTRIMEWQKFIPAFFIKWNVRNTFLK
                     LEKELFHQDYEQFYLPGESKPKNIGEPFFLKRFFSRKGIILVHGYMAAPEEIRPLADY
                     LHQNGYNVYGVRLRGHGTAPEDLAIRNWEKWYDSASRAYIIMKNSVKTFSIAGFSMGG
                     GIALLQAANKPGRFASVISINAPMVLRGMASKFAPAVVAWNTLLKKINIDKWKMEFIK
                     NDPENPDINYLRNPVSGSYELEKLMKRVDHHLKNIADPVLIIQASNDPVVDPCSGRDI
                     FNKIGGGNKQFFMVNANHHGILRGKEATEVHAKVLAFLSESASK"
     gene            4503..>4816
                     /locus_tag="PLF77_05185"
     CDS             4503..>4816
                     /locus_tag="PLF77_05185"
                     /inference="COORDINATES: protein motif:HMM:NF014448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HPC08283.1"
                     /translation="MIEQWMTEIKRTAPADMLGMILSHNGIVRATAKTGESVQAMHLS
                     YDKTKLEKAVREFKGKEGIVDIRVWINEGHLKIGDDIMNVCVAGRFRKDVLPVFQELI
                     SM"
     assembly_gap    4817..4916
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4917..5312)
                     /locus_tag="PLF77_05190"
     CDS             complement(<4917..5312)
                     /locus_tag="PLF77_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08284.1"
                     /translation="MIRHAVVMVSAICFWGAAFLTVPAYADYRIILKNGETISVSNYE
                     MDGDNIVFNKYGGLVSFSRSGIAKIEEDNIEEDIPEENMAEDDANEADDSEQPAKNKV
                     SKKSGQKSEDPKNDREKKIAECQRILKMKK"
     gene            complement(5416..6609)
                     /locus_tag="PLF77_05195"
     CDS             complement(5416..6609)
                     /locus_tag="PLF77_05195"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08285.1"
                     /translation="MLTSDLDTTRLAIIGPGRLGTAFAYKFGRDNKRVAIYYHDAEVC
                     KAINRDHLNPLHLTEDLAGRLGGMEKVPRLADKVYATNDLEHIVENNDFIILTITMNR
                     LPEFLNYLKPMIDKKAGDTCLISPIKGLISDEISKELITPSQLINNSLYNLKHKYQIG
                     CIGGPFFDMDIALGNPVCLTVAGKKRIANIIREDLLKFNRKEINSYYNFDIVGVEACG
                     ALKNVVANIKGLADSLQLGDSIPGTIFARSGVEIRSLSRLMGGSFQAFHSQAGVGDMY
                     VTVSSLASKNYRYGKYFYELYTGNPIETNLRVLEKIDGTPEGPNTVRNVHKYLEKKNM
                     YSPLFSCAYHIFNEAGEKGAIRDLIIQACQYDKRNKEYIDAFSRLMYRILPDFWYRRD
                     KSWFD"
     gene            7121..7786
                     /locus_tag="PLF77_05200"
     CDS             7121..7786
                     /locus_tag="PLF77_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HPC08286.1"
                     /translation="MEKQTLGKEITQEFHDGKIKEKKYVRQGKTEGEYFSFYHSGQIL
                     AHVNFIRGKKNGPAVIYYHDGHIREKATFVNDRMEGEYVSYFENGHIREHEFYRNGKL
                     DGKYEIYYKTGQLKLVETFRNGKFEGEYIAYYKNGQIKEKGFFKNDKREGEYIAYYKS
                     GQIREKATYKNGNCVGKFLAFEEEISGSGETNGSESDTEIWDEKDVANLLEELKHFDK
                     KQK"
     gene            7813..8664
                     /locus_tag="PLF77_05205"
     CDS             7813..8664
                     /locus_tag="PLF77_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPC08287.1"
                     /translation="MISNILIPTDGSANSQTALDYGIYIARKLAASLTGLYVIDVNLI
                     QGPMVTDISGSIGMPPYEGFFESIEKSLNEKADFILKEFEERCRKSGVKAATDRKTGK
                     ISPIIIEEAQKADLILMAKKGEHFHLKEGGLLGSVAESVTRNSGKPVLVTPEKFVEIE
                     SMALAFDGSESATKALGLSLNLSHKTSWPLTVVIVSPDEKKAEALSGKVDKMIQQNPD
                     EASIDCETIILSGKEQDEIVKFIREGSVELMVMGAYGHNRLRELLLGSTTSHVIHKSP
                     IPVLLIR"
     gene            8719..9918
                     /locus_tag="PLF77_05210"
     CDS             8719..9918
                     /locus_tag="PLF77_05210"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPC08288.1"
                     /translation="MEHRYGKNILPLSTFDNENFIGSPPLEILFFYKKDLPILKLKES
                     LLKTIHYYNLFSSRLIMIGDHKFALLYCTDGAVANDLRPVDACFDDSRIAEIRKEMVH
                     VQTLPGEPLFAVTGIPFKNGILAGISCSHAVADGISLVLFLYAWMCITDGKNFLPPSP
                     QRLFQGPLAHFDPTDRALVPPLSALNDQIREKVDIDNVAEIFTATEYFTDDFLNAIKA
                     EAEKENYDISNHQIMTAFLLKKYHRKILPDTDRIVLKNPINLRDIHPDIDPMYIGSSN
                     FNNFIQFTKEEIDRLSISQIASRIKKSIADVRNESYAREIAHLSPYGIEINMDVFRRN
                     RPPYSVGTNVVSSNLTHLNDLESMFVGPETGSLLHIGLAVQTGFTMLKEKSGRIFAQI
                     TGRHPLT"
     gene            complement(9985..10752)
                     /gene="surE"
                     /locus_tag="PLF77_05215"
     CDS             complement(9985..10752)
                     /gene="surE"
                     /locus_tag="PLF77_05215"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPC08289.1"
                     /translation="MRFLLTNDDGIYAKGLSALYKELSKEADCLIVAPETEQSAVGHA
                     ITISRPLMVRRATKNNQFLGYAVCGTPADCVKLGIKELSDHPVDLVVSGINRGGNAGI
                     NVLYSGTVSAATEAAIMGFPSMAVSLDTHKEADFTFAAKFARKMTRMILNHREQLLGS
                     AINVNVPCLPQGKIKGVVVVRQAQTNIVEYFEKRTDPRENTYYWFAGESSSATRQKDT
                     DVGALTSGFITVTPIHFDLTRHDLLDQLKDALEKNRF"
     gene            complement(10816..11400)
                     /locus_tag="PLF77_05220"
     CDS             complement(10816..11400)
                     /locus_tag="PLF77_05220"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HPC08290.1"
                     /translation="MNVLSFRKKYFYLPVLVFLISGFIPGVFAAGKERQANQCKTVIL
                     ANEEFLPALLNAIDGAQDEIIMSIFSFKAGVHKNSYPDRIAGHLAKAVKRGVKVFVVL
                     ENTGDSSDALNIQNRKAGKFLAGKGIRVYFDSPRKTTHTKLTVIDQRLIFLGSHNLTQ
                     SALKYNNEISVLLEGRDLALNARNYILGIIKEGT"
     gene            complement(11509..13431)
                     /gene="dnaK"
                     /locus_tag="PLF77_05225"
     CDS             complement(11509..13431)
                     /gene="dnaK"
                     /locus_tag="PLF77_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPC08291.1"
                     /translation="MGKIIGIDLGTTNSCVAIMEGGDPVVIANQEGNRTTPSIVAISE
                     SGERLVGQVAKRQAITNSENTVYAVKRLIGRKYTSKEVQYDLKIIPYKITEASNGDAQ
                     VTVRGKNYSPAEISAMILTKMKQTAEDYLGEKIKDAVITVPAYFNDSQRQATKDAGKI
                     AGLNVMRIINEPTAAALAYGLDKKKDEKIAVFDLGGGTFDISILELGDGVFEVKSTNG
                     DTHLGGEDFDLRVMDFLVAEFKKDQGIDLRNDKMALQRLKEAAEKAKMELSTTMETDI
                     NLPFITADASGPKHLNIKLTRAKLESLVEELINQVVGPCQTALKDAGMSPKDIDEVIL
                     VGGMTRMPRVQQKVKEIFNKEPHRGVNPDEVVAIGAAIQGGVLSGDVKDVLLLDVTPL
                     SLGIETLGGVMTRLIEKNTTIPTKKSQIFSTAADNQPAVSIHVLQGERQMAADNRTLG
                     RFELVGIPPAPRGVPQIEVAFDIDANGIVHVNAKDLGTGKEQSIKITASSGLSDADIE
                     KLVKDAEMHAEEDKRRKELIETRNQADSMVYSVEKNIKEFGDKIDSAEKTKIEDAIAR
                     TKKALEGDDVEAIKKAQDDLMNVSHKLAEAMYAKSAGSQGGPGADGGAQQQSGDQQAS
                     GKKNDDVVDADFEEVK"
     gene            complement(13442..14047)
                     /gene="grpE"
                     /locus_tag="PLF77_05230"
     CDS             complement(13442..14047)
                     /gene="grpE"
                     /locus_tag="PLF77_05230"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPC08292.1"
                     /translation="MKSKKEQSEHSAKGFQAGEHKDKDAAEDRNKEVEALKQKLEEKE
                     NEATANYDRYLRAVAEFDNYKKRALKDKADTIKYANEEIIKDILPFMDSLDRALEQEA
                     GDVQTFREGIALIQEQLLCCLKKYGVERIDAAGQDFDPNFHEALMQMESDQHEDNKVV
                     SEMEKGYLLNGRLVRPARVCVCKKTKKQEDDMIEIMEDTGE"
     gene            complement(14076..14660)
                     /locus_tag="PLF77_05235"
     CDS             complement(14076..14660)
                     /locus_tag="PLF77_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HPC08293.1"
                     /translation="MEDYIIKIFKESNRVKDNFLNENLGKLANVIEVITSALKAGNKI
                     LLFGNGGSAADAQHLAAEFVNRFIIERPPLPAIALTTDTSVITSIGNDYDFSEIFSKQ
                     VRAIGQPGDIAWGISTSGNSANVLKGLEMAKKMGLVTVAFTGKDGGDIAKIVDFSINV
                     SSNSTARIQETHITAGHAICELVDIKLFQKPDFQ"
     gene            14932..15372
                     /locus_tag="PLF77_05240"
     CDS             14932..15372
                     /locus_tag="PLF77_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPC08294.1"
                     /translation="MFGPNLWRMKRFADSVPELIDLQREINRLFSSVGQAAGADYPAI
                     NVWEKEDSLVVTTELPGMDPDDINISVTGSMLTISGKSKGDPLKAGETYLRQERELGN
                     FQRNVQLPFQVDSPAVEAKYEKGVLNITLPRQKEDLPRKIQITA"
     gene            15390..15782
                     /locus_tag="PLF77_05245"
     CDS             15390..15782
                     /locus_tag="PLF77_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPC08295.1"
                     /translation="MSDKDLQKTENTAATERIRNVKTFVPRVDIYETKDALFLIADMP
                     GIDEQTVDVELEKNILTISGRVENGKVKDYRLVFSEYEVGDYERTFTLSDEIDRDKIK
                     ANVKHGVLRLELPKAEEVKPKKIKINAA"
     assembly_gap    15936..16035
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(16212..17063)
                     /locus_tag="PLF77_05250"
     CDS             complement(16212..17063)
                     /locus_tag="PLF77_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HPC08296.1"
                     /translation="MVGTLRKSLKTIEDYKSANPHYTELLDIMADILILREEYLKNMS
                     RPIFHVDENLIAQKMTGGLPLIDLTDKEFDLKRPRDYFYALIQAAEKRIPELEQSISD
                     IVDDPDFDWEKSVRASFDCKVEEADARTAEADREEENNIDLIDLFLEESLRPELEIIA
                     KKYGETVAKSDWTEGYCPICGKEPKIGEIRKDEEGKRYLFCHQCGFKWYFYRIKCPFC
                     GNDEQQSLAYFEVEGEERYRVDVCNKCWRYIKTVELPKFSDEPNMDVEDIATLHLDMI
                     AYDEGYN"
     gene            17505..17948
                     /locus_tag="PLF77_05255"
     CDS             17505..17948
                     /locus_tag="PLF77_05255"
                     /inference="COORDINATES: protein motif:HMM:NF022072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HPC08297.1"
                     /translation="MFRYEFVTQIEIDATAERVWNVLTDLDSFPQWNPMIRWAKGKVC
                     SGERLTVRFEPKGTRGYTFTPKLLIVEPNRQLRWLGYPRIPAFMDTEHYWLIEQITGE
                     KSRLLHGAIIYGLLTPVSRIIFENTSRGPFEQMNHAHKKRAESRE"
     gene            complement(17962..>18204)
                     /locus_tag="PLF77_05260"
     CDS             complement(17962..>18204)
                     /locus_tag="PLF77_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08298.1"
                     /translation="GCIVTDKISKKDLMQDMKKAVKSGVPRVDVLKTGATLCIACFDC
                     GAACPHGAISIKRNFDPKYFYKRLTQLSDMRLPRKY"
     assembly_gap    18205..18304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18305..18494)
                     /locus_tag="PLF77_05265"
     CDS             complement(<18305..18494)
                     /locus_tag="PLF77_05265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPC08299.1"
                     /translation="MKKFSVIDRWRIPTYDNPNHVKTADLFFNSKKCKQCGTCVKICP
                     GGCIVTDKISKKDLMQDMK"
     gene            complement(18496..19251)
                     /locus_tag="PLF77_05270"
     CDS             complement(18496..19251)
                     /locus_tag="PLF77_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPC08300.1"
                     /translation="MSSKDDVLSPLGKYEEGMAWNPVEKVIMERRSIRAFKKEPLPDR
                     MIERILEAGRFAPSAGNSQPWKFIVVNSPEILAEMEKDALKIIKFFMFFIDYTRGNIL
                     RRLYILSWVKWIIRILKNKLHPVPFGLMSQIAQGRAPVFHGAPTLILLAEDRRGVASP
                     PTDIGICGQNMVLTAHSLGAGSCWIGLIQVLMYLPKWRKKFGIKYPYKLNECIALGWP
                     NPRVDGMVPREVQLVQWYERGMNDAPRITKQGE"
     gene            complement(19305..21020)
                     /locus_tag="PLF77_05275"
     CDS             complement(19305..21020)
                     /locus_tag="PLF77_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPC08301.1"
                     /translation="MYLLANLKIGSSMIKSLLTFFKDEIRRGTFRANFSSLMKTQEIT
                     EDMSWAELSEEKARQYGDRTFLIYEDREFTFRQMDENANRVANFLQSLGAGKAKGVAI
                     IMANCPEYLDVYIGSQKLGMYSIPINTSLRGDSLLYILNHSDAEFIVIDEEFLDAYNK
                     IADRLERKKTVIVNRSVPPGASGLPHGLLNLADAYKQSPRKPNVPYDKNDVCFILYTS
                     GTTGLPKGVMYRYGKTTLKLLSIPAYALYKRSDILYTCLPLFHGNALWLCVTQGMHRG
                     CRVVLGRKFSASRFWDDMRKYKITAFNTIGAMIPILMKQPPRDNDSVNSVRFTLSAAC
                     PVDEWGKFERRFGIKIYEAYGSVDGGGKSIMNLGNAPVGSIGKPAPNTVYRLVDNEGL
                     DVPDGTPGQLIFESKGKKKSVEYFKNEKASNDKLRNGWIYTGDLVKRDKNGYLYFVGR
                     NAEFMRIKGENVSAYEVEHTIQKHPSVLEAAVYAVPSELAEDEIMASVSLVEGQTLKE
                     ADLIAFLKDDLAKFAVPRFVRIVREFPKTETQRIIKKELEKIGVVAGTYDAQKNQYVG
                     NTKIA"
     assembly_gap    21197..21296
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21297..22360)
                     /locus_tag="PLF77_05280"
     CDS             complement(<21297..22360)
                     /locus_tag="PLF77_05280"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPC08302.1"
                     /translation="MLTTVKCELKSFEDLAHNFAAKELAANREANDRYPFGPLFDSVI
                     DKAHDVGLLGATLPEECGGIGQDIGALCIILDHISAADASLAGIIFTNALAQEIVLAA
                     GDKNLLTALMEKAADARAALIAFPSFSNPAENGVSLEAVKSKDTYLLSGAMEYIVLGG
                     LAGQALVPVKIKGEEGFSFFLIRPADKGVTKSEPVFSLGLHACPAVDLTLSAVEGQLI
                     GQAGEGIKYFNQASDRMHAAAAAIQCGIMRGSFQEAFDYSRQRCQGGREIINWSGLRM
                     LLAQMAVQVKIAEMVVSEAAMAVENQEKQWGLCTRAAALHLSELACKLTADGIQCLGG
                     NGYMKDYGQEKRFRDAKHVQA"
     gene            complement(22372..23880)
                     /locus_tag="PLF77_05285"
     CDS             complement(22372..23880)
                     /locus_tag="PLF77_05285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPC08303.1"
                     /translation="MIKPKEVKSLLDREPRFEDSFCSVQAIPKIPKIQLKETRTLVSH
                     ARRFNDEVVRPLALKTDLKTFQDHDYLPWEIIEKANEWGFYTMFIPKIFGGQGWSLPS
                     HAYVIEELSSACVGIANVAFVHYLGFMTLLASWNLPLINRVCREVAEGEKTGKPCLIS
                     LAITEPGAGTDVEEVELVDRGKVTCFAEKVKGGYIVNGSKIFISMGHVSTWHALIAYT
                     DLKKPSANTLMLAVRTGMKGFTFGKHEDKMGQRACCASELIFQDCFIPDEYVLGDSRD
                     FPNSQIRETSDLVNQIIHYVVEVTRASVGAFGTGVARGAYEHALKFAGETEVDGKLLI
                     NHEWAQIMLAEMYKNVIAGRLAYNEANYSNSLKGGIFNFLQVKPLYYYLKYVPQIVID
                     TFIVPFYRFKMTDWAIRQIFVEGQSMEAQKRCSGWSSIAKFFGTDMGLKNSQMALELM
                     GQAGLRHDRGAEKFLRDSKLLQIYEGTNQLNRLNLFNCLIARCVPQARVFEE"
     gene            complement(23840..>24065)
                     /locus_tag="PLF77_05290"
     CDS             complement(23840..>24065)
                     /locus_tag="PLF77_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08304.1"
                     /translation="PCVFIFLKHPHPEKIYVTRDVLQLERKRFSVITVSGSLVCRRGS
                     SLQKGKIVAVNPQREGDYDKTEGGQIFIRP"
BASE COUNT         6114 a         5921 c         5385 g         6245 t
ORIGIN      
        1 aacagatttt agaaaaatac cttcagcccc agcgttacca ttcccgtgga aaacgtgacg
       61 tcgtcggaat cctcatggtc aaattccatc tcggtggcaa aataacgata accgacatcc
      121 acagcaattt cactggttaa gaatatttcc agtccggtca tgatctgcca ggcataagac
      181 gtgtaatccc cgtcatcaat tccccggata ccgtcccagg acgtatccgc ataggaaaat
      241 cccaacccca gcccaaaata gggttgaatg attccgaggg gatatttccc gatgatgttg
      301 aacatcaggt tgctcatctt gacatcgccg tcaagcacat agtcatcttc tacttcaggg
      361 tttaaataga ggtattcggc ttccagggca agatacttaa aaaaaggatt ggtataaccg
      421 atcttggcgc cccaggcgga cgtttcgtca aaatccagat cgacatcatc ataataatcc
      481 cagtcatctt ctgttgcatt agggtgaatt gtatagcctc cccatccacc aagatataat
      541 ccaacctgag ccgaagccgg ttgaaaagga tagacagcaa acaaaacggc cagggtaatc
      601 atggcagcat attgaacaat ttttttcaca gtgattctcc ttttcgcaaa ttattttgtt
      661 gtattctaat gttttatatt ccgcttttca atatgttttt cagaataaaa caggcatcag
      721 ggtgttgcag cttttttgcc cagcttaaaa gctttttttc tggctttttc atctttgacg
      781 atttcccctt tatcgttggc agaggccata actgcaccca tccagcgcat ttttgtcata
      841 tccgaagttg atttaaagct gtggacgatt ggatcagccg tgtgaacatt cggatctccg
      901 cagacggtaa tcaaaccgat gcgctttcct ttcattttcg gataataaga tttctgccag
      961 ctccaggtcg cgtcaaacag cgcgcaccac cggtccagat acgctttcat ctgcgcggtc
     1021 atcaaccaga aataaaccgg cacgctgtga ataatagcat ccgcctccag aatctttttg
     1081 tgaatggccg gcatatcatc cttgacgacg cagaggccgg tgtcgttgca ttttttgcaa
     1141 tccagacatc cggatatttt ctttttgacc agaacaatct tttccacccg agcgcccgcc
     1201 tcttcggcgc cggccagggc gtcgtcgagc agaatatcgc tgttgccgcc gatccgcgga
     1261 cttcccataa tgccgagaac tttcatagag tcatcctcct tgttcataag aattctattc
     1321 aagacgcccg ctcatttatg tatcataaat cctttcggta tggtttgtaa acactaaaat
     1381 aattgaagaa ctctgctctt aaatatggta aaaaaaaaca tgcctgaatc atttcatttt
     1441 ttcagctatc accggcgcga acaggatgat tttaccgtca tcgcccatcg cggagcttcg
     1501 gcttattatc cggaaaacac catgccctcc tttgaaggcg ccatcgtcat gggtgcggac
     1561 atggcggaac tggacgttca gctcaccgcg gatcaggaag tcgttgtgtt tcacgacgaa
     1621 aagatttcac gctgcacaaa cggccgggga agaatcgccg attacgatct tgcgtattta
     1681 aaacagttgg acgccggcaa atggtttgat aaaaagttta aaaacacgcg catccccgaa
     1741 ctggccgacg tgctggcact ctgcaaaaac cgcatcgccg taaatattga aataaaaacg
     1801 gaagcggtgg acgacatcat cgccggcggc attgaagaaa aatgtctgca actggtcgag
     1861 cagtacggca tgaaaaagca cgtcgtattt tccagttttg atccgcgcgc gatcctgcat
     1921 ctaaaacaga tcgacaaaac ggctccggtg gctgttttat ttgaaaagaa gctttacggt
     1981 tcccgcctgc cttcggatat tgtcaaatcg ttgcatgccg atgcatttaa ttgctccgca
     2041 tccgaattga ataaaaaatg gctggcagac ctccactcga acaatattcc ggtaaatatt
     2101 tatacggtca acaacgaaaa aaagatgaga caatatttaa agctgggcgt cagcggaatt
     2161 tttaccaaca aaccggatgt tttaaaaaac gtcctggcgg atggagatca gaaaagcaaa
     2221 aaaacaaatc tatgaacata tcaaaagacc tggtcaattt tatcatgttc tcgctggaca
     2281 ccctgattca gacagcaggg atcgacatcc gtctgcacgg cctggagaaa gtccccgatc
     2341 agccggttgt ctatgtgatc aatcatttta cgcgtctgga gacggtcttg atgccgtatc
     2401 taatcaaaaa gcatctccac aaagaagccg tttccctggc gcatgagtct tttttcagcg
     2461 gtaaaatgaa agattttatg gataacgtgg gcgctgtttc caccgccgac ccccataaag
     2521 ataaaatatt cattcatgcg cttctttccg acagacatcc cgtaatcatt tttccggaag
     2581 gacagatcat taaggataaa aagatcgtcg agaaaggaaa atatattgtt tacaacaccg
     2641 gcatccgccg tcccccccat tccggtgcgg cgcgcatcgc tctgctgacc gagtttatcc
     2701 gcgaaaagtt gcgaacgctg aaagcgcgta acgatatggc atccatcgcc cgcatcgcat
     2761 cgcgctttga tttttcagga aatgatctgg agaaaattat tcgcaaagaa acatatatcg
     2821 ttccggtgaa cattacctat tatcccgtcc gcgcccggga aaacgccctg agccggctgg
     2881 ccggaaaatt tttcaaaaac atgtcagccc gcctgcagga ggaactagag gtggaaggcc
     2941 ccatcgtgct gggcaaggtg gatatcgaca tcaacttcgg caggcccatc gcaatcaaac
     3001 gttatatcca ttcaaaatgg aaaatgaaaa gaatgatggc ggatggaaat gtttatctga
     3061 atacaggtga tttcaaaaga atatccgcat tccgtcaagt atgcgtcaga ttgatgtacg
     3121 attacatgaa tgccatatac ggcatgacca ccgttaatca cgaccacatc atgtcttata
     3181 ttctgacgag ctaccctcag ggcagcttca gcgaaaacga ctttaaaaaa cgtattttcc
     3241 tggccattga acatttgagg aaaatcgccc tgcccaactg ccatacatcg ctttacaaaa
     3301 agcaatttta cctgctgacc gacgatcagc acgaaaagta tgagagtttc atgaacgaag
     3361 ccgtcaccaa caactatctc cgaagagaaa acggcgtcat tacaaaaaac aaagaacggt
     3421 tcagcgtgcc atccgacttt cataccatcc gcagagacaa cattatcgag gtgctccgaa
     3481 atgaaatcaa accgctgcgt catttcacca ggatcatgga atggcagaaa ttcattccgg
     3541 cattttttat taaatggaac gtcagaaaca cgtttctcaa actcgagaag gaactttttc
     3601 atcaggatta tgaacagttc tatttaccgg gtgaaagcaa accgaaaaat atcggcgagc
     3661 cttttttcct gaaacgtttt ttcagccgca aaggaattat tctggtgcac ggttatatgg
     3721 cggcgccgga agaaatcagg ccgctggcgg actatctgca tcagaatggc tataacgttt
     3781 acggcgtgcg cctcaggggc cacggtacgg cgcccgagga tctcgccatc cggaactggg
     3841 agaaatggta cgactccgca agccgcgcat acatcatcat gaaaaatagc gtcaaaacgt
     3901 tttcaatcgc cgggttttcc atgggcggcg gcattgcgct tttacaggcg gccaataaac
     3961 cgggccggtt tgcatcggtc atatcgatca acgccccgat ggtattaaga gggatggcgt
     4021 caaaattcgc gccggccgtc gtcgcctgga atacactgct caaaaaaatc aacattgaca
     4081 agtggaagat ggaattcatt aaaaatgatc cggaaaatcc ggacattaat tacctccgca
     4141 atcccgtcag cggcagttat gaactggaga aactgatgaa aagggttgat catcatttga
     4201 aaaatatagc cgacccggtg ctgatcatcc aggcctcgaa cgatccggtt gtggatccct
     4261 gcagcggccg ggatattttc aataaaatcg gcggcgggaa caaacagttt ttcatggtca
     4321 acgctaacca tcatggtata ctgcgcggca aagaggcgac cgaagtccat gccaaggtcc
     4381 tggctttttt aagtgaatcc gcttcaaaat aatttattta tcccggcgaa ttcacgaaag
     4441 tgaacgtggt actctctaca ggagttgccc gtaaaaacag caaaatcatt aaggggaata
     4501 ttatgattga acaatggatg acagaaataa aaagaacggc tccggccgat atgctgggga
     4561 tgattctttc ccacaacggc attgtccggg cgacagcaaa aaccggcgaa tcggttcagg
     4621 cgatgcacct ttcttatgat aagacaaagc tggaaaaagc ggtgcgcgaa tttaaaggaa
     4681 aggaaggcat cgtcgatatc cgggtttgga tcaatgaagg tcacctgaag atcggcgacg
     4741 acattatgaa tgtttgcgtg gccgggcgct tccgcaaaga cgttttgcct gtttttcagg
     4801 aactgatttc catgatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncttt
     4921 ttcatcttca gaattctctg gcattccgca atctttttct cccgatcatt cttcgggtct
     4981 tcgcttttct gcccggattt ttttgacact ttattctttg ccggctgttc agaatcatcg
     5041 gcctcattcg catcatcctc tgccatgttt tcttccggaa tatcctcttc gatattatcc
     5101 tcttcgattt tagcgattcc cgaacggctg aaactgacca ggcccccata tttgttaaaa
     5161 acgatattat cgccatccat ttcgtagttg ctgacggaaa tggtttctcc gtttttcaat
     5221 ataatccggt agtcggcgta cgccggaaca gtcaaaaaag cggctcccca aaaacaaatt
     5281 gcagaaacca tgacgacggc atgtctgatc attcgcgatg tctcccgtaa aatgaaagga
     5341 atgctgtgct gatttttatc tgtcctcttc ctttattttt aatattattt tgcaaacagt
     5401 gtttaatgat cccttttagt caaaccaact tttgtcgcgg cgataccaga aatccggaag
     5461 aatacggtac attagccggg aaaaagcatc gatgtattct ttgtttctct tgtcatactg
     5521 acaggcctgg atgatcaaat ctcttatcgc gcctttttca ccggcttcat taaatatgtg
     5581 atacgcgcag gagaaaagcg ggctgtacat attcttcttt tccagatatt tgtgcacgtt
     5641 tctgacggta ttggggcctt cgggcgttcc atcgattttc tccagaacgc gcagattggt
     5701 ttcgatggga ttcccggtat aaagttcata aaaatacttt ccataacggt aattcttgct
     5761 cgccagcgag gaaaccgtta catacatgtc ccccacgccc gcctgactgt gaaaagcctg
     5821 aaagcttcct cccatcaggc gggacaacga acgaatctcc acaccggacc gggcaaagat
     5881 ggttccgggg atggagtccc caagttgcag ggaatccgcc agtcccttga tattggccac
     5941 gacgtttttc aaagcaccgc aggcttccac gccgacaatg tcaaagttgt agtaggaatt
     6001 gatttccttg cggttgaatt tcagcaaatc ttctctgatg atattggcga tgcgcttctt
     6061 gccggcaacc gtcagacaca ccggattgcc cagcgcgata tccatgtcaa aaaacggtcc
     6121 gccgatgcat ccgatctgat atttgtgctt caggttatac aagctgttat tgatcagttg
     6181 cgacggcgtg atcagttctt tggaaatttc atcggaaatc aatcctttaa tcggagaaat
     6241 caggcaggta tcgcccgctt ttttatcaat catcggcttg agataattga gaaattccgg
     6301 caagcggttc atcgtgattg tcagaataat aaaatcgtta ttttcaacaa tatgttccag
     6361 atcatttgtg gcatacacct tgtccgcaag acggggaacc ttctccattc cgcccagacg
     6421 gccggccaga tcttccgtca aatgcagagg atttaaatga tcccggttta tcgccttgca
     6481 gacttccgca tcatggtaat aaatggcaac ccgtttatta tcacgaccga atttataggc
     6541 aaaagctgtg cccagacggc cgggaccgat aatagcaagc cttgtggtat ctaaatcaga
     6601 cgtcagcatc aatcaatctc gttgtgaaaa aattcgtctt tatggcaatg caaaacacat
     6661 taccggtttt taatatcttc ttttaaaaaa aaattaaact aaatttatta atgagcactg
     6721 attccctacc catgcgtttc ggggaaatca agagtatttt cgatattctc cgctaatgcg
     6781 ggatagttca actaataaac cttgctgcgc tctgcctgca atgcttgagt ttcactaatt
     6841 cgaccaacgg ctcaaacgtt cttcactatt cattatcccg ctccgctgcg cttcggggat
     6901 aattcgacta acgcttcgaa cgtcgttgca ctcgttctca gcgagtctca ttatttttgt
     6961 tgcattatca taaatgattg tttaaaaaaa gcaatgggtt gatttcagaa gcatggcaga
     7021 ggaccacgaa gcaacacggc aagaatattt tcgtaaaacg atcttcagaa ttttaaactt
     7081 gcattcatcc aattgaaggc aaaaaagaga ttatatttta atggaaaaac aaacacttgg
     7141 taaagaaatc acgcaggaat ttcacgacgg gaaaattaaa gaaaagaaat atgtgcggca
     7201 aggcaaaacc gaaggcgaat atttttcttt ctaccatagt ggccaaattc tggcccatgt
     7261 caatttcatc cgcgggaaaa aaaacggccc cgcggtcatt tattatcacg acgggcatat
     7321 cagagagaag gcaacctttg tcaacgaccg gatggaaggc gaatacgttt cttacttcga
     7381 aaacggccac atccgggagc atgagtttta cagaaacggc aagctggatg gaaaatatga
     7441 gatttattat aaaaccggcc agttaaagct ggtcgaaact tttagaaacg gcaagtttga
     7501 aggtgaatat atcgcctatt acaaaaacgg acaaatcaag gaaaaaggat tttttaaaaa
     7561 cgacaaacgg gaaggtgaat atatcgccta ctacaaaagc ggacaaatcc gggaaaaagc
     7621 cacttacaaa aacggcaact gcgtggggaa atttctggcc tttgaggaag aaatatccgg
     7681 ctccggggaa acaaacggca gcgagagtga caccgaaatc tgggatgaaa aagatgttgc
     7741 caatttgctg gaagagctga aacattttga taaaaaacaa aagtgaatgt tttatcgcca
     7801 ggaggcgtca ttatgatcag taacatttta attcctacgg acggttccgc aaacagccag
     7861 acggctttgg attacggaat ctatattgcc cgtaaacttg ccgcctctct gacaggcctt
     7921 tatgtcatcg atgtcaatct gattcagggg cctatggtga cggatatttc cggttcgatc
     7981 ggcatgccgc cctatgaagg attcttcgaa tccatagaaa aatcgctcaa tgaaaaagcc
     8041 gatttcattc tgaaggaatt tgaggaacgc tgccggaaat ccggtgttaa ggccgccacg
     8101 gacaggaaaa ccggcaaaat cagcccgatt attatcgaag aagctcaaaa ggccgatctg
     8161 attctgatgg caaaaaaggg agaacatttt catctgaagg aaggcggtct gctggggtcg
     8221 gttgccgagt cggtaacacg caattccgga aagccggtat tggttactcc ggaaaaattt
     8281 gtagaaatag aaagcatggc actggctttt gacggcagtg agtcggcaac aaaagccctg
     8341 ggtctttctt tgaatttatc ccacaaaacc tcctggccac tgacggtggt gattgtcagc
     8401 ccagacgaga aaaaagccga ggcgttatcc ggaaaggtcg ataaaatgat tcagcagaat
     8461 ccggatgagg cctctattga ctgcgaaacg atcatcctgt ccgggaaaga acaggatgaa
     8521 atcgtcaaat tcatccggga aggctctgtg gaattaatgg tcatgggggc ttacggtcac
     8581 aatcgtctgc gcgaattgct gctcggcagt accacatcgc acgttatcca caaaagtccg
     8641 attcccgtat tgttgatccg gtaacctgaa gtttgttcaa tgtccgagtc gacaataccg
     8701 gcaattgaat agaaaaaaat ggaacatcgt tacggcaaaa acatccttcc gctgtcgaca
     8761 ttcgataatg aaaatttcat cgggtcaccc cccctggaaa ttttattttt ttataaaaaa
     8821 gatcttccca tattaaagct caaagagagt ctgttaaaga cgatccatta ttacaatctt
     8881 ttttccagtc gactgatcat gatcggcgat cataaatttg cgcttctgta ttgcacggat
     8941 ggagccgttg caaatgatct gcggcctgtc gacgcctgtt ttgatgacag ccgcattgcc
     9001 gagatcagga aagaaatggt gcatgttcaa accttgccgg gcgaacctct ctttgctgta
     9061 accggtattc ctttcaaaaa cgggatactg gccggcatca gttgctctca cgcggtggcg
     9121 gatggtattt ccctggtgct ttttctttac gcctggatgt gcataacgga cggaaaaaat
     9181 tttcttccac cgtcgcccca gagattgttt caaggcccgc tcgcccattt cgatccaaca
     9241 gacagagcgc ttgttccccc cttatccgcg ctgaacgatc aaatccggga aaaagtagac
     9301 atcgataacg tcgcagagat tttcaccgcc acagaatact ttaccgatga ttttctcaat
     9361 gcaattaaag cggaagcaga aaaagaaaat tacgacattt ccaaccatca gatcatgacg
     9421 gcgtttttat taaaaaaata tcaccgtaaa atcctgccgg acaccgaccg gatcgtttta
     9481 aaaaatccca tcaatctcag agacatccat ccggatattg atccgatgta tatcggcagt
     9541 tcaaatttca acaacttcat acagttcacc aaagaagaaa tcgacaggct ttcgatatct
     9601 caaatcgcgt ctcgtattaa aaaatcaatc gctgatgtgc gcaatgaaag ttatgcgcgg
     9661 gaaatagcac atttatcccc gtacggaatt gaaatcaata tggatgtctt cagaagaaac
     9721 cgccctccct acagcgtggg aacgaatgtc gtctccagca acctgacgca tctgaacgat
     9781 cttgaatcca tgtttgtggg accagaaacc ggaagcctgt tgcatatcgg cctggcagtc
     9841 caaaccggat ttaccatgct gaaagaaaag agcgggcgga tctttgctca gataaccggc
     9901 aggcatccat taacctgatg ccgataacct ttacgggcgg tgacgcgatg tccgtcatcg
     9961 ctggctcggc aatcagccaa tgtcttaaaa cctgtttttc tccagggcat cttttaactg
    10021 atcgagcagg tcatgtctgg tcaaatcaaa atggatcggt gtgactgtaa tgaatccgga
    10081 cgtcagcgcg ccgacgtcgg tatctttctg ccgggtggcg gaagacgact ccccggcaaa
    10141 ccagtaatag gtattttccc gcgggtccgt ccgtttttcg aaatactcca cgatattggt
    10201 ctgcgcctga cggacaacca caacgccctt gatctttccc tggggaaggc agggcacgtt
    10261 gacattaatc gcgctgccca gaagttgttc ccggtgattg agaatcatgc gggtcatttt
    10321 acgcgcaaat ttggcggcaa acgtaaaatc cgcttctttg tgcgtgtcca gagaaaccgc
    10381 catggacggg aaacccataa tcgccgcttc ggttgccgcg gaaaccgttc ccgaatacag
    10441 cacgttgatc ccggcattgc cgccgcgatt gattcctgaa acgacgagat cgacggggtg
    10501 atccgataac tctttaatgc ccagtttcac acagtctgcc ggcgttccgc aaacggcata
    10561 ccccaaaaat tgattgtttt tggtcgcccg gcgcaccatc aggggacggg aaatcgtaat
    10621 ggcgtgtcca accgcgcttt gctccgtttc cggtgcgaca atcagacaat cggcttcttt
    10681 ggaaagttcc ttataaagag cgctcaatcc cttggcgtag attccatcat cattggtcaa
    10741 caaaaaacgc attatttttc atcttctccg atcttgtttt tttcggccgg caacggaaac
    10801 agactgcctg cacgttcatg ttccttcttt aataattccc aggatataat tacgggcgtt
    10861 tagcgccaga tcgcgtcctt ccagcaggac ggaaatttca ttattatatt tcagcgcgga
    10921 ttgcgtgaga ttatggctgc ccaggaaaat cagccgctga tcaatcaccg tcagtttcgt
    10981 atgcgtggtt ttgcgcggcg aatcaaaata aacccggatc ccctttcctg ccagaaattt
    11041 tcccgccttt ctgttttgaa tattcagcgc atccgatgaa tctcccgtat tttccagaac
    11101 cacaaaaacc ttcacccccc gttttaccgc tttggccaga tggccggcga tacggtcagg
    11161 ataactgttc ttgtgtactc ccgccttaaa agaaaaaatg gacatgataa tctcatcctg
    11221 cgctccatcg atggcgttca acagtgcggg cagaaattcc tcattggcca gaatgaccgt
    11281 tttgcattga tttgcctgcc gttctttgcc cgcggcaaaa acaccgggga taaatcccga
    11341 aatcaaaaaa accaataccg gcaggtagaa atattttttt ctgaacgaca atacattcat
    11401 gatcctctcc cctttatggt attgtatatt atttcagcat tccgcgcgat ataaaaaagc
    11461 ccgaaacggc agcagccatt ccgggctttg gaaacactat ggacagcact atttcacttc
    11521 ttcgaaatcc gcgtcaacca cgtcgtcgtt tttcttgccc gatgcctgct gatcgccgct
    11581 ctgctgttga gctccgccat ccgctccggg acctccctga gaacctgcag acttcgcgta
    11641 catcgcttcc gcgagtttgt gggatacgtt catcaggtcg tcctgagctt tcttgatggc
    11701 ttcaacatcg tcgccttcca gggctttctt tgtcctggca atggcatctt caatcttggt
    11761 cttttccgcc gaatcgatct tatcgccgaa ttccttgata ttcttctcga cagaataaac
    11821 catcgagtcg gcctgatttc tggtttcaat cagttctttg cggcgtttat cttcttccgc
    11881 gtgcatttcc gcgtccttga ccagtttttc aatatccgcg tcgctcaaac cgctggacgc
    11941 cgtgatctta atactctgct ccttgccggt ccccagatcc ttggcattga catgcactat
    12001 accgttggcg tcaatgtcaa aagccacttc gatctgcggt accccgcgcg gcgcgggcgg
    12061 aatacccacc agttcaaagc gccccagcgt ccggttatcc gccgccatct ggcgttcgcc
    12121 ctgaagaaca tgaatgctca cagccggctg attatccgcc gctgtggaga aaatctggct
    12181 tttctttgtg gggatagtcg tatttttttc gattaatctg gtcatcacac cgccaagcgt
    12241 ttcaatgccc agagaaagcg gggtcacatc cagaagcaga acatccttaa catcgcccga
    12301 caagacgccg ccctgaatgg ccgcgccgat ggcaacaact tcatcggggt taacgcctct
    12361 gtgaggctcc ttgttgaaaa tttccttcac tttctgctga acgcggggca tgcgggtcat
    12421 cccgccgacc agaatgactt cgtcaatgtc tttgggagac atgccggcat ctttcagcgc
    12481 cgtctggcat ggaccgacca cttgattaat cagctcttca accagggatt ccagcttggc
    12541 gcgggtcagt tttatattca aatgtttcgg accggaagca tccgcggtga taaacggcag
    12601 gttgatatcg gtttccatgg tcgtggaaag ctccatcttg gctttttccg ccgcttcttt
    12661 caggcgttgc agggccatct tgtcgtttct taagtcaatg ccctggtctt tcttgaattc
    12721 ggcaaccaga aaatccatca cgcgcagatc aaaatcctca ccgcccaaat gggtatcgcc
    12781 attggtcgat ttgacttcaa acacgccgtc gcccagttcc agaatcgaga tatcaaatgt
    12841 tccgccgccc agatcgaata cggcgatctt ttcgtccttc tttttatcca gaccgtaggc
    12901 cagcgcggca gcggtcggct cattgatgat acgcatgaca ttgagtccgg caatcttgcc
    12961 ggcatctttg gtcgcctgac gctgagaatc gttgaagtaa gccggaacgg taatgactgc
    13021 gtccttgatt ttttcgccca gataatcctc ggcggtctgt ttcattttgg tcagaatcat
    13081 cgccgagatt tccgccggtg aataattctt gccgcgcacg gtgacctgcg catcgccgtt
    13141 ggaagcttcc gttattttgt agggaattat ctttaaatca tactgaacct ctttcgatgt
    13201 atatttccgg ccgatcaatc gcttgacggc atagacggta ttttccgagt tggtgatcgc
    13261 ctgccttttg gcgacttgtc cgaccagacg ctctccgctc tcagaaatgg ccacgatgga
    13321 cggtgtcgta cggttccctt cctgattggc aataaccacg gggtcgccgc cttccataat
    13381 agccacgcat gagtttgtgg ttcctaagtc aattccaata atttttccca ttgtttcctc
    13441 cttattctcc tgtatcctcc atgatttcaa tcatatcatc ttcttgtttt ttagttttct
    13501 tgcacacaca tactctggcg ggtctgacca gccggccgtt taacaaataa cctttttcca
    13561 tttcactgac gactttgttg tcttcatgct ggtcgctttc catctgcatc aacgcttcat
    13621 gaaaattcgg atcaaaatcc tgaccggcgg catcaattct ttccacgcca tattttttca
    13681 aacagcataa aagctgttcc tgtatcaggg cgatgccttc cctgaaggtc tgaacatctc
    13741 cggcttcctg ttccagggcc ctgtccagac tatccataaa aggcaggatg tctttaataa
    13801 tttcttcgtt agcatattta atggtatcgg ctttatcctt gagcgccctt tttttataat
    13861 tatcaaattc cgcaaccgca cgcaggtatc tgtcataatt ggcagtcgct tcattttcct
    13921 tttcttcaag cttttgcttt aatgcttcaa cctctttatt tctgtcttca gcggcatctt
    13981 tatctttatg ctcaccggct tgaaaacctt tagcgctgtg ttccgactgt tcttttttac
    14041 ttttcactgc accctcatga atcatgcatg taatattatt gaaaatcagg tttctgaaat
    14101 aattttatat ccaccagttc acagatcgcg tgacccgctg taatatgcgt ttcctgaatt
    14161 cgggcggtgc tgttcgaaga aacattgatg gagaaatcga ctattttagc gatatctcct
    14221 ccatctttgc cggtaaaagc caccgtgaca agacccattt ttttggccat ttccagaccc
    14281 ttcaaaacat tggcagaatt gccgctggtg ctgatgcccc aagcaatatc accgggctgg
    14341 ccgatcgctc tcacctgttt gctgaatatt tcggagaaat cataatcatt tccgatactc
    14401 gtaatcaccg atgtgtccgt ggtcagagcg attgccggaa gcggaggtct ttcgataata
    14461 aagcggttga cgaattcggc cgcaagatgt tgggcatctg ccgctgaacc gccattaccg
    14521 aacagcaaaa ttttattgcc cgctttcaat gccgacgtta tgacctcgat aacattcgcc
    14581 agttttccca aattttcatt tagaaagtta tctttgacgc gattactttc cttaaaaatt
    14641 tttattatgt aatcttccat atttatatca ttaagccaag tgtcaaattc cgtttcctga
    14701 agcggactta atatatttac ggggtttggg catgtcaagg gaatgacaaa aatttcttat
    14761 ttttattcag tagatatttt gccacgagcg cagatattat cgacaatcgg agatggttgc
    14821 gaatcttgac aaataatttt tcgtaaatat atttaaagtg aaaacagtga aataattaat
    14881 aatagcgaat agttataaaa aaataatgtt ttcatgaaag gaggatggtc tatgttcggt
    14941 ccaaatcttt ggagaatgaa aagattcgcc gacagcgttc cggaattaat tgatttgcaa
    15001 agagaaataa accggctttt ttcaagtgtc ggccaggctg ctggcgcaga ttatcccgca
    15061 atcaatgtct gggagaaaga ggacagtctg gtggtgacaa cagaactgcc gggaatggat
    15121 ccggacgata tcaacatttc cgttaccggt tccatgctga ccatttccgg aaagagtaag
    15181 ggcgatcccc ttaaagcagg ggagacttat ctgcgtcagg aaagagaact gggcaatttc
    15241 cagcgcaatg ttcaattacc cttccaggtg gactctccgg ctgttgaagc aaaatatgaa
    15301 aaaggggttc tgaacattac cctgcccaga caaaaagaag atttgcccag aaaaattcaa
    15361 attactgcat aaaaggaggt cgttgaatta tgtccgataa agatctgcaa aagacagaaa
    15421 acacggcagc cacagaaaga atacgcaatg ttaaaacttt tgtgccgcgt gtggatattt
    15481 atgaaacgaa agacgctctt tttctgattg ccgatatgcc cggaatcgat gaacaaacgg
    15541 ttgatgtgga gctggaaaaa aacatcctca ccatttccgg acgtgtggaa aacggcaagg
    15601 tcaaagacta tcgccttgtc ttttccgaat atgaagtcgg cgattacgaa aggacattca
    15661 cactgtcaga cgaaatcgac agagacaaaa tcaaggcaaa cgtgaaacac ggtgtattaa
    15721 ggctggaact gcccaaagcg gaagaggtaa aaccgaaaaa aattaaaatt aacgcggcgt
    15781 aacaaaacgt gaagagttgc gtcattacat ttttaaagaa aggaggaagt gacggatatg
    15841 atgattaaga atttattacc cgatgtcgtc cgaagaacaa gagctgacct tgatcaacct
    15901 ttttattccc tgcagaaaga aatgaacaat ctgttnnnnn nnnnnnnnnn nnnnnnnnnn
    15961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnngaatc gggcaaagcg gaggcgcgtt ttaaaaacgg aattctggac
    16081 atcaagattc ccaagagcca gagtgccaaa gcgaaaggga ccaaggtttc catcaagagc
    16141 ggctaatctg caaagcccgc ctcttttcca aggggcgggc ttttccagaa tcttaaacgt
    16201 tttcttgggg cctaattgta cccttcgtcg taagcaatca tatccagatg caacgtggca
    16261 atatcttcaa catccatatt gggttcgtcg ctgaatttgg gcaattccac tgttttgatg
    16321 tagcgccagc acttgttgca gacatccacc cggtagcgct cttcaccttc cacttcaaaa
    16381 taggccagtg attgctgttc gtcgttgccg cagaaagggc atttgatgcg gtagaaatac
    16441 catttgaacc cgcactgatg gcagaacaga tatcgtttgc cctcttcatc ttttctgatt
    16501 tcgccgatct tcggttcctt gccgcaaatc ggacagtagc cctccgtcca gtcggatttc
    16561 gcgaccgtct ctccgtattt tttggcgatg atttccagct ccggcctgag actttcttcg
    16621 agaaaaagat cgatcagatc aatattgttt tcttcttccc ggtccgcctc cgccgtccgg
    16681 gcatcggctt cttcaacctt acagtcaaag gacgcgcgaa cacttttctc ccagtcgaaa
    16741 tcgggatcat caacgatatc ggaaatgctt tgctccagtt caggtatacg tttttccgcc
    16801 gcctgaatca gggcgtaaaa atagtcccgg gggcgtttta aatcaaattc cttatccgtc
    16861 agatcaatca agggcagtcc gcctgtcatt ttctgagcaa ttaagttttc atcaacatga
    16921 aaaatgggac gggacatgtt tttgagatac tcttccctta aaatcagaat gtcagccatg
    16981 atgtccagga gttccgtata atgaggattg gctgatttat aatcctcaat cgtttttaaa
    17041 gatttgcgca gtgtaccgac catgctttca tactcctgtg agacgtttat taagatttgt
    17101 tatcctgaat gtcactgaat aatccgattt tcattagaac aaaactgatc gacctatatc
    17161 cactttccca tgtatcttca agtagttttt tcacaggcaa caaaactgcg gcggcgacat
    17221 caactcccgg ataaaagcat ccataagtat ccggatatcc tatggatatc ttttgacaag
    17281 acttctgcaa atcatgacaa aatgctgccg gcggcatccg acggcaaata aaagccgaac
    17341 gatgcgcaaa aacgcgctct gtcggcaaaa aactccagcc atccggccgg gtctatttca
    17401 tctttgggtt taacgtgcca tttttgcctg cggctttaat ccacttgaca cacaggatac
    17461 cgtttcatat agtccagact cgctaaaagg aagttttacc ataaatgttt cggtatgaat
    17521 tcgtaacaca aattgagatt gacgcgacag cggagcgagt atggaacgtc ctcaccgact
    17581 tggattcctt tccccagtgg aatccgatga ttcgctgggc aaaaggaaaa gtatgctcgg
    17641 gagagaggct gaccgtccgg tttgaaccga agggaaccag gggatatact tttacgccaa
    17701 agctgctgat cgttgaaccg aaccggcaac tgcgctggct gggatacccc cggatacctg
    17761 cattcatgga taccgaacat tactggctca tcgaacagat aaccggcgaa aaatcccgtc
    17821 tgttgcatgg ggctatcatt tacggcttgc tcacaccggt gagccggatt atttttgaga
    17881 acacctcccg gggtcccttt gagcagatga accacgctca caaaaaacgc gccgaatcac
    17941 gtgaatgaat agtgatccgg attaatattt tcttggcaac ctcatatcag acaactgagt
    18001 cagcctctta taaaaatatt tcggatcaaa atttcgtttg atggaaatgg cgccgtgggg
    18061 acaggcggcg ccgcagtcga aacaggcgat acaaagcgtg gctcccgtct ttaagacatc
    18121 cacacgaggc accccgcttt ttacggcctt tttcatgtct tgcatgagat ctttcttgga
    18181 aattttgtcg gtaacaatgc atccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18301 nnnnttttca tgtcttgcat gagatctttc ttggaaattt tgtcggtaac aatgcatcca
    18361 cccggacata tcttgacaca tgtgccgcac tgcttgcatt ttttagaatt aaagaataaa
    18421 tccgccgttt tcacatgatt cggattgtca taggtcggta tccgccatcg atcgatcacg
    18481 ctgaattttt tcatatcatt ccccctgctt ggtaattctt ggagcgtcgt tcatgcctct
    18541 ttcgtaccat tgaaccaact gcacctcgcg cggcaccatg ccatcgaccc tcgggttggg
    18601 ccaaccgaga gcgatacatt cgttgagctt ataaggatat ttgatgccga atttttttct
    18661 ccatttaggc agatacatca atacctgaat gagaccgatc cagcagcttc ccgcccccag
    18721 gctgtgcgcg gtcaggacca tgttctgccc gcaaattccg atatcggtgg gcgggctggc
    18781 cacaccccgt ctgtcctccg ccagaaggat caacgtcggc gcgccgtgaa agacaggcgc
    18841 ccggccctgg gcaatctgcg acatcagccc gaacggaaca ggatgaagtt tgtttttcaa
    18901 aatccggatg atccatttga cccagctcag aatatagagt cggcgcagta tattgccccg
    18961 ggtgtaatca atgaaaaaca tgaagaactt tatgattttc agcgcgtctt tttccatttc
    19021 tgcaagaatt tcagggctgt tgacgacaat gaatttccag ggttgtgaat ttccggctga
    19081 cggggcaaac cggccggctt ccagaattct ttcgatcatg cggtccggca acggttcttt
    19141 tttaaaagcc cggatgctgc gccgctccat gatgactttt tcaacgggat tccaggccat
    19201 gccttcttcg tattttccca acggagacaa tacgtcatct ttcgaactca tatttcctcc
    19261 ttacggttga tttactgcaa accttttgag acgtccttga aacgttatgc aatcttcgtg
    19321 ttacccacgt actgattttt ctgggcgtca tacgttcctg ccacaacgcc gatcttctcc
    19381 agttcttttt tgatgattcg ctgtgtttcg gttttgggaa attccctgac gatgcggaca
    19441 aaacgcggaa cggcaaattt ggccaaatcg tctttgagaa aggctatcag atccgcttct
    19501 ttcagcgttt gcccttccac gagagaaacg ctcgccataa tttcatcctc cgccagttcc
    19561 gacggaacgg cgtagacggc cgcttccagc acggaaggat gtttctgaat ggtatgttcg
    19621 acttcgtacg cggaaacatt ttcgcccttg atgcgcatga actccgcgtt gcgtccgaca
    19681 aaatagaggt agccgttttt gtcgcgcttc acgagatcgc cggtgtagat ccagccgttg
    19741 cgcaatttgt cattgctggc tttctcgttt ttaaaatact ccaccgattt tttcttgcct
    19801 ttggattcga aaatgagctg tcccggcgtg ccgtccggaa catccaggcc ttcgttgtcc
    19861 accagccggt ataccgtgtt gggcgcgggt ttcccgatag agccgaccgg ggcgttgccc
    19921 aagttcatga tgcttttgcc gccgccgtcc acggaaccgt acgcttcata aattttgatg
    19981 ccgaatcgtc tttcgaactt cccccactca tccaccgggc aggcagccga caacgtaaag
    20041 cgcacgctgt tgacgctgtc gttgtcgcgg ggcggctgct tcatcagaat cgggatcatg
    20101 gcgccgatgg tattgaaggc ggtaatcttg tatttgcgca tgtcatccca gaaccggctg
    20161 gccgaaaatt ttctgcccag aacgacccgg caaccgcggt gcatgccctg tgtcacgcac
    20221 agccagagcg cgttgccgtg aaacagggga aggcaggtgt aaagaatgtc cgatcttttg
    20281 tacagcgcgt acgcgggaat ggaaagcagc ttcaaagtgg ttttgccgta gcggtacatg
    20341 acgcccttgg gcaggccggt tgttcccgac gtataaagga tgaagcacac gtcgttttta
    20401 tcatagggca cattcggttt ccggggcgat tgtttataag catcggccag gttcaacagg
    20461 ccgtgaggca gaccggaggc acccggcggt acggagcggt tgacaattac cgttttcttc
    20521 cgttcgagcc tgtccgctat tttattgtac gcgtcgagaa attcttcgtc gatcacgatg
    20581 aattccgcgt cgctgtggtt caggatatag agcagactgt cgccgcgtaa agaggtgttg
    20641 atgggaatgg aatacatgcc aagtttttgc gacccgatgt acacatcaag atattcggga
    20701 cagttggcca tgatgatggc gacacccttt gcttttccgg cgccgagcga ctgcagaaaa
    20761 ttggccaccc tgttggcgtt ttcgtccatc tgccggaatg taaattcccg gtcttcgtaa
    20821 atcagaaatg tccggtcgcc gtactggcgg gctttttctt cgctgagttc ggcccacgac
    20881 atgtcttccg taatttcctg cgttttcatc aggctgctga aattcgcccg gaacgtcccg
    20941 cgcctgattt catccttgaa aaatgtcaat aaacttttga tcatggacga accgattttc
    21001 aaatttgcca gcaaatacat cttcactcct catccttctt ttttagtcat ccggaacccg
    21061 tgacagcgcg cgtggagatt ccactatgac aacatgtccc tgatcagaga cagcttccgc
    21121 atgggagcca tccccagcag cgcctggaca tgcttggcgt cgcggaatct tttttcctgt
    21181 ccgtaatctt tcatatnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcct
    21301 ggacatgctt ggcgtcgcgg aatctttttt cctgtccgta atctttcata tagccgttgc
    21361 cgcccagaca ctggatgccg tcggcggtca gtttgcaggc cagttccgac aggtgcagcg
    21421 ccgccgctct ggtgcaaagc ccccactgct tctcctggtt ctccaccgcc atggccgcct
    21481 cggaaaccac catctcggcg attttcacct gcacggccat ctgagccagc agcatgcgca
    21541 atcccgacca gttgatgatt tcgcggccgc cctgacaacg ctgccggctg taatcgaacg
    21601 cttcctgaaa cgatcccctc ataataccgc actgaatggc cgccgccgcc gcatgcatcc
    21661 ggtcggaggc ctgattgaaa tatttgattc cttctcccgc ctgaccgatc agttgtcctt
    21721 ccacagcgga aagcgttaaa tccacggcgg gacaggcatg cagtcccagg ctgaagacgg
    21781 gctcgctttt ggtaacgcct ttatccgccg gacgaatcag gaaaaagctg aagccttcct
    21841 ctcctttaat tttaaccgga acaagcgcct ggccggccag gccgcccagg acaatgtatt
    21901 ccatggcgcc gctcagcaga tatgtgtctt tggatttaac ggcttcgagg gaaacgccgt
    21961 tttccgcagg attggaaaaa gatggaaagg cgatgagcgc cgctctggcg tcggccgctt
    22021 tttccatcag agcggtcagc agatttttat cgcccgccgc gaggacgatt tcctgagcca
    22081 gagcgttcgt aaaaataatt ccggccaggc tggcatccgc tgcgctgatg tggtcgagaa
    22141 tgatgcacaa ggccccgata tcctggccga tgccgccgca ttcttcgggc agcgtcgcac
    22201 cgagcagtcc gacgtcatgc gctttgtcga tcacggaatc aaagagggga ccgaaaggat
    22261 aacggtcgtt tgcttcccgg ttggcggcaa gttccttcgc cgcaaaattg tgtgccagat
    22321 cttcaaaaga cttgagttcg cattttacgg ttgtcagcat tctcatccct cctattcttc
    22381 gaatacacgg gcctgcggca cgcaacgcgc gatcaggcaa ttaaaaagat taaggcggtt
    22441 caactgattg gtgccttcgt aaatctgcag cagcttcgaa tcgcgcagga atttttccgc
    22501 gccgcggtcg tgccgcaatc cggcctgccc catcaattcg agcgccatct ggctgttctt
    22561 caaccccata tcggtgccga agaatttggc gattgacgac cacccggaac agcgtttctg
    22621 cgcctccatc gattgtcctt ccacgaaaat ctgacgaatc gcccagtcgg tcattttaaa
    22681 ccggtagaag ggaacgatga atgtatcgat cacaatctgc ggaacatact tgagatagta
    22741 gtaaagcggt ttcacctgca ggaaattaaa aatgccgccc ttcaggctgt tggaataatt
    22801 ggcctcgtta taagccagcc gtccggcaat cacattttta tacatctcgg ccagcataat
    22861 ctgcgcccac tcgtgattga taaggagttt gccgtccacc tcggtctcgc cggcaaattt
    22921 cagcgcatgt tcataagctc cgcgcgccac gccggttccg aaggcgccta ccgacgcacg
    22981 ggtcacctcg acaacatagt ggatgatctg attgacgaga tcggaggtct cgcgaatctg
    23041 ggagttgggg aaatcgcgtg aatcacccaa aacatattca tccggtatga aacaatcctg
    23101 aaaaatcaat tcgctcgcgc agcaggcgcg ctgccccatc ttgtcttcat gcttgccgaa
    23161 ggtgaatccc ttcatcccgg tcctgacggc aagcattaac gtattggcgg aaggtttttt
    23221 cagatcggta taggcgatca gggcgtgcca ggtggataca tgtcccatgg aaataaaaat
    23281 cttgctgccg ttgacgatgt atccgccctt gaccttttcg gcaaaacagg tcaccttgcc
    23341 gcgatcgacc agttccactt cctccacgtc cgtaccggcg ccgggttcag taatcgccag
    23401 cgagatcaga cagggcttgc ccgttttttc tccttcggcg acttcacgac agactcgatt
    23461 gatgagcggc aggttccagg aagccagcag ggtcataaaa ccgaggtaat gaacaaacgc
    23521 gacgttggca atgccgacgc aggccgaaga caactcctca atgacgtaag cgtggctggg
    23581 gagactccag ccctggccgc cgaaaatttt ggggatgaac atggtataga atccccattc
    23641 gttggccttt tcaataattt cccagggaag atagtcatga tcctgaaagg tttttaaatc
    23701 ggttttcagc gccagcggcc tgacgacttc atcgttgaag cgacgggcgt gactgacgag
    23761 cgtcctggtt tccttgagct ggattttcgg aattttcgga atggcctgca cactgcagaa
    23821 actgtcctcg aaacgcggtt cacggtctaa taaagatttg acctccttcg gttttatcat
    23881 aatccccctc cctttgtgga ttgactgcaa caatcttacc cttttgaaga ctgctccccc
    23941 ttctgcaaac cagggagccg gataccgtga taacgctgaa tctttttctt tcgagctgta
    24001 aaacgtcacg ggtgacgtaa atcttttcag gatgagggtg cttcaagaat atgaatacac
    24061 atggc
//
LOCUS       DAOOLB010000028        23721 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_2025_length_23721_cov_38.451703, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000028 DAOOLB010000000
VERSION     DAOOLB010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 23721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23721
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2025_length_23721_cov_38.451703"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1071..2024
                     /locus_tag="PLF77_05295"
     CDS             1071..2024
                     /locus_tag="PLF77_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08305.1"
                     /translation="MKSMSVSSASEQRGHQSDSTDPLPVNAQDFFIQSHKGFNYYLID
                     LNTAEWNRLGDIIRGCEAVENDCWKAQEEFSTYLRKCDVLSYVEKNGRIVAFDAVSIL
                     NSGPHCVYNNEETMVLKAFRGRDLAQRLVIMTVEWHLTKAASIKNKDYFIFTSISANP
                     AVVNRYFNKSWLRVLFDSSFSPSPALIALKEEYCRKYGIGLVDQSYPFCLKNMFPGSN
                     GFDPNDPKYQFSPHVRANMPADFEHMDRGDAFAFMLMIPMKIVRLVTFVLMIKCFGKE
                     YFSSRGVGLFSSRRPLIPSFTDRRVPGLSGEPFSVKTADHG"
     gene            2110..3483
                     /locus_tag="PLF77_05300"
     CDS             2110..3483
                     /locus_tag="PLF77_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08306.1"
                     /translation="MIKLQKGYLKISCVLMCLLALIFATRTWACDVAVVSGKVTTDGK
                     PLIWKNFDNSSSDRQQVAYFPAKKSGPGGYLMVYRFENGMKLLNNGSAITPSGGVNES
                     GFAIAGTSVYQDYNLMAEPVNINTVLMEEALATCTTLAEFESLLKRWPYYHWGTVISA
                     NFVVIDAKGGAALYECFTGHLNTGLNLMQFRKNDANTGKITNQYGWTLKRAQSNFIGF
                     HIMTNYNYYIPWNVGQDRKYRAEALLTQLATEKRINYRTVMREVAKDVVGKQVQTDLS
                     SETNYSTTYCLSRNATRSSIVVRGVASGDNPRLATMWVNLGEPSVGVFVPFFAAARGT
                     SELARVDTYANGAWRDYDDSCLLNQAISARETYGSLIYSSNEGDVIWGMYDNYINKVE
                     LAKVQQWTFQIEDTIIDNAESFLSSLQSNPAQITAFNLKDFADYCADYAYENFETGSA
                     DAFPWQY"
     gene            3575..4603
                     /locus_tag="PLF77_05305"
     CDS             3575..4603
                     /locus_tag="PLF77_05305"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPC08307.1"
                     /translation="MDKTAKISNSQAVMGDMDFPHEERARPFLMNTKLWKQLLPLAVS
                     GAILFYLFRHIDIRMCFNAMLTANMSLYIPSLIALILITFLLDTQNLAETLKHFHYPL
                     SWKNAFTLRGVTYLIMTIDYSVGMGVLIYYLKNHLGIPVMRSTGLMTFFNGITQKALV
                     YMAIIGLVILSPASGLLRNLLIFFIGFALLDILFIIVLKKLPSRGLALKIKSMNLLKV
                     FHEASWRTYGVLIFWRILYYACFVAFFYVAVRAFNMQIPLVALIAYVPIILLVISLPI
                     APGGFGTAQATMLLLFKDYGTPLNIMAFGLIYTTSILVLRYLIGLLYAKHIKGLPAGE
                     DSTSEQRN"
     gene            4729..5538
                     /locus_tag="PLF77_05310"
     CDS             4729..5538
                     /locus_tag="PLF77_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08308.1"
                     /translation="MSRALHLAVVILVTAFSIQTAVAQEHFSHALGHFETLSTAQGIL
                     IAAPHGTYDIRTDVLAKDVARTLGAGYVIAGGFASPSSRINVNRPSEGAGHTCEKERR
                     TERARDVYDQYARLIDKIRDGKPLRLYVEIHGHTSIPFLNRLEIATTGITTEEARRLK
                     DRFPAFLAQAKAAYPAYPDLELRIEPLDKIYFGARCNKTTGYLSTRNMERALHMEIPR
                     PARTPEALKATAALVSSIVRATMTISESASGPGPKRATSDADKKSSGGPAR"
     gene            5535..>6026
                     /locus_tag="PLF77_05315"
     CDS             5535..>6026
                     /locus_tag="PLF77_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08309.1"
                     /translation="MTKRYTRKMTFNDRAFVVLNQISPPTVNQFIFDGDGTLDPEKWR
                     RAADAASEANPGTRLVLRGHLSWSRWVDSGINMRVREVDGRNWDGRGPENEPLLEESL
                     PYRGNPTCEIVLIQGPTPRVAFRSHHAVCDGRGTLFWAEDVFRTLRGEAVVGSTSNIT
                     DVEL"
     assembly_gap    6027..6126
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6127..6877
                     /locus_tag="PLF77_05320"
     CDS             <6127..6877
                     /locus_tag="PLF77_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08310.1"
                     /translation="LRGEAVVGSTSNITDVELCKSFQNRYRTPFPAEHIAPAGRAKGN
                     DTGVTWRRCSLKGKMPHLLAQCARLAAEEAWRHGEGVVRFGIPVDLRHRRPELRSTAN
                     LSFALYTEVKKETSPDDIARDIALQISRCDECRLTKGDERLEYMPLWMMRYKAKNIIR
                     DRHRRGLYSLSGILSNLGRINLNRFCGGGFTARAFWIIPPAAEYYPFFMVMTGYEGGS
                     DLTISMPKVLASEGRLENTLKRIVHGLEGTN"
     gene            6892..8088
                     /locus_tag="PLF77_05325"
     CDS             6892..8088
                     /locus_tag="PLF77_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08311.1"
                     /translation="MTYSRQLSSTERAYVVYNECCPPFLNQLFWEGTGYFDLPKWREA
                     VKIASEANPGSRVVVKGITAFARLVDTGKTPPVIEVDGSHWDGLGPEGAPAKLSETLN
                     PFEGPTSEVVLIHGNPLRVVFRTMHATMDGRGTVTWVEDIFRGLRGEAPLGSDSRITE
                     FEIAATFQKQGRKGPVPEYIAPTGMPQGDQPGVIWRRRRYPGRYHNVLGQVATLLAGK
                     ARQYQEGKVRLAIPVDMRARVEGLRSTGNLTNLIYVEINPSTTPADVSQDIKRQLEAR
                     HDGMIYWGDRPIRFLPLWLLKKLIKSNIATKNRTGRYPNSGVLTNMGRIPVFSGGGFE
                     GKYTWGIPPGQEYSPIFIGMCNSDDDMTMIYSMPNVLAGGGRLETLMDHIQQGLVASK
                     ADRKKG"
     gene            8098..>8621
                     /locus_tag="PLF77_05330"
     CDS             8098..>8621
                     /locus_tag="PLF77_05330"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPC08312.1"
                     /translation="MWISDTSIKRPVLATMFILALVVLGLVSYPSIGVDLFPKVEFPL
                     VNITTGLAGASPEIMDVDVTDKIEEAVSSINGVKTIHSLSIDGVSSVIVEFELERDID
                     LAVQDVREQISAVRSQLPDDIIEPVIQKVNIDASPVLWLALTGDKSQRELSTYVDESL
                     KEKLQKIQGVGALSL"
     assembly_gap    8622..8721
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8722..10656
                     /locus_tag="PLF77_05335"
     CDS             <8722..10656
                     /locus_tag="PLF77_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPC08313.1"
                     /translation="YRHIEDGMSPREAASFATDEIGPAVMATTLAIVVIFLPVAFMKG
                     IIGRFFMQFSLTVVFSILVSLLVSLTLTPMLSSLLLHPRKKESLDSERPGTVMARIGQ
                     TLERGYKKIEEAYRPILTFSLDHRRAVLMSALILFIFSLWITRFIGKEFKPGEDQSQF
                     MIRMESPIDYSVEHAGAMFSQAEKIVRNRTEVTSVFYGQGAGQGRLSQINQAFIQTSL
                     KPRSDRDKSQSEIMAELRKEFAAIPGLKTTIEEVAVLGGGIRNVPVQYSISGGSIDEL
                     RKYAKQIVGELSKIKGLVDIDTSIEAGKPELSVYIDREKATNMGVSVAAVTEAVNLLM
                     SGEVEVTKFKDTAKGRRYDVRMRLDAKDRMSPSDIGRLYVRAVNGELVRLSDIVTVEE
                     GGGPSSISRKDRQRTIWIFANLQGKPLGEAMREIDAISARILPAGYVAGYSGEAEEMG
                     KSFKYLMFALLLGILLAYMVLASQFESLLHPVTILLSMPLSFIGAFAALLIFGKSLSI
                     VSFIGLVLLMGLVKKNAILLVDFTNTLRERGLSRREAILQAGPVRLRPILMTTFAMVF
                     GMLPVALGIGEGADLRAPMGITLIGGLLTSLFLTLAVVPAAYDIFDDWKENMIRRLGS
                     RGISPLIRPFGKNKKGNNNN"
     gene            10669..11985
                     /locus_tag="PLF77_05340"
     CDS             10669..11985
                     /locus_tag="PLF77_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08314.1"
                     /translation="MMKTAILLYHFFKSLIEIHVSARLPYGLQRKLCERRLIRSIRHA
                     YDHVPFYRQKYDAAGVDITSIRSADDIKRLPFVTKEEIRQNFPDGIVARGVNREACRY
                     SATTGSTGRSLPFIFTQATYAFYVALGVRMYTMIGYRPWHKMAYIKYTDLNYTRLGPF
                     FRAVHIKSTIPVEEQIAQLRKAGADLIIGYASLVYEIARNATPDDLKDIRPKFIGINS
                     ELSTEAQRTFISETFGCPVYDEYSTEETWMIASQCRYNNYHIFTDNVWVEFIDSKGQE
                     VAPGDMGEIVLTTTRSPAMPFIRYRIGDMGRFSNAECPCGMGFPLLQSFEGRADDCFI
                     LPSGKFVSSLKLLNTFTMYIKKYLHLLEEFKVTQVHSDLIVIKIVKGNQYDSAHLEEL
                     LADLRRLLDDSVSIKTEFVDVIEKTDGIKRKAIESLVSQKQTGSPS"
     gene            complement(12368..13708)
                     /locus_tag="PLF77_05345"
     CDS             complement(12368..13708)
                     /locus_tag="PLF77_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HPC08315.1"
                     /translation="MQLIESVLQQISDILWGWPLIVLLVGTHIFLTFRLKFIQRYMFK
                     AIKMSISRKREGEGDISQFGALTTALAATIGTGNIVGVATAIAAGGPGAVLWMWLTGV
                     FGIATKYGEAVLSLKYRITNKEGLMAGGPMYVLERGMNARWLGLIFAALTAVAAFGIG
                     NMVQANSIASLVQETFNISPWITGLTMTVLTAVVILGGIKSIARVCEKLVPFMAVFYV
                     FGCLILLGMHYETIPETFKLIFQSAFSGHAVIGGFLGAGMKEAIRYGVARGLFSNESG
                     MGSAPIVAAAAQTKNAIRQALVSSSATFWDTVVVCAITGLVLVNSGEWIHGYKGAALT
                     KAAFSALPVVGPFVLSIGLLTFVFSTILGWSYYGEKAAEYLLGTKAVRPYRYLWVGAV
                     MLGSVLSLPIVWTFADIANGLMAVPNLISLLVLNKVIVSETKHFLWEGKLDEPR"
     gene            14216..14983
                     /gene="xth"
                     /locus_tag="PLF77_05350"
     CDS             14216..14983
                     /gene="xth"
                     /locus_tag="PLF77_05350"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HPC08316.1"
                     /translation="MKLATYNVNSIRSRMHIIVPWLKAHSPDFFCMQETKVADTVFPA
                     AEFEALGYHVVFKGDKQYKGVAIVSKQKPQKVIYGFDSEPSDPDRMIIAVFNDLTIIN
                     TYVPQGQEMQSSQFTYKLEWLARFKKYLQKNFRPQNELIWCGDLNIAPERIDVHDPKR
                     LLGHVCFNPDVWTALKDIKSWGLTDVFRKHHPGIAGQYTFFDYRIKDSVKRGLGWRVD
                     HILATKALAERSKSSAIDLDSRLAEKPSDHLILYAQW"
     gene            14993..15505
                     /locus_tag="PLF77_05355"
     CDS             14993..15505
                     /locus_tag="PLF77_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08317.1"
                     /translation="MDKKENIERINTNIADRFDKVEKKLSGAGSIAELFEMLLTQIEA
                     EFQVPFIWISVADTIKTGRVIDAFKSSRILKDRLNVIKADQFREIFFSGLQPILVNKN
                     LRPYYRLFPPNQKYFIKSLAMVPFKINGEVAGSWNNGDAIQSRYSPDMETSLLQKLSR
                     RLSARLDELP"
     gene            complement(15584..15859)
                     /locus_tag="PLF77_05360"
     CDS             complement(15584..15859)
                     /locus_tag="PLF77_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08318.1"
                     /translation="MRKLMQTTLLTAFVVLVTTSFSYAQYSVTGDANFPFFHLGCLIV
                     GGLIIISLKRKYTKLYMSEAIGSFALYALLVSIFTAPVVDALKNLIN"
     gene            16195..16815
                     /locus_tag="PLF77_05365"
     CDS             16195..16815
                     /locus_tag="PLF77_05365"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC08319.1"
                     /translation="MERLERKEREFNMRRAEILEKAEKIFSVKGYHNVTMAEIASASG
                     FSTGSLYQFFEGKENLYSSMISEKLNLMYEETKKATDRADNVLDKIEMLVDAQLRFLE
                     ENADFCRLFIKGESTALSEAMTSMREKIIKDYFKYIGFIENLLKEGIQQKLLKNLPAG
                     DMAQSLVYLIRAVSMEWMLSLKNEFPYSKKGFILDIFFNGVKNHDR"
     gene            16805..18148
                     /locus_tag="PLF77_05370"
     CDS             16805..18148
                     /locus_tag="PLF77_05370"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPC08320.1"
                     /translation="MIGKKIRILTIVVMIVFSLRLSFAQEVLSLEASIDLALKNSNIL
                     NIAKEGLNSATAQKREALTGFLPKFSTAYSYSRLNEAPFSRFQGLPPGPMYDLNGMET
                     PVGTKDNYNWIIEARQPLFAGGGILANYQASSIGEEVARLEETARIQDVVRDVKAAYF
                     NILRAQKINEATAQSVEMLKAHRDVAENYFKVGLIPKNDLLFADVELANGRQALVRTQ
                     NAVELAKSRLNTLLKRDISAPVDVMDILAYQPLKQTFEECLETARRLRPEMKISSLKV
                     QQAGKLVRMSQSEYYPALSLVGNYTRFGDEASVHGSDFKDAESWYIMAVANWNFWEWG
                     RTKFRVDASKARENQASESSKELIDQIALEIKNAYLLLLEAQNQISVSETLIKQAEEN
                     FRISEERYKERVATSTEVLDAQTLLTRAKSEYASALGDYNISLAHLQRAMGNIWP"
     gene            18169..19584
                     /locus_tag="PLF77_05375"
     CDS             18169..19584
                     /locus_tag="PLF77_05375"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPC08321.1"
                     /translation="MMKKSLLCMMTVLATALLSAANAVAQEYTLREIYQKALHTSEKM
                     EIARENVYVAQMSKDKALSLLIPRLTAYGTYNRFTEDKYNTANIMIQPEDSAAWGVRA
                     DQTFSLSARELDALRIAGQSISKSEYDLDWAKSDFLLAVASSFYDVLKAQKALDITVS
                     NLERLTKYYHLVETRMKVGELTKTALLRAQGELSGAKSDYLKASNALKLARAALTRLT
                     GVEPDFRLKEETVSLSEACDIQGLRKSASDLRTDLKSYDMQTKMAGQQVKYARGAFWP
                     SVGLFAIYNRADQDPAGSTFNDENIYGGISLTFPFFEGGLRMAEFREARAKERQARLA
                     YDDLKKSVDIEVEAACLDLDTLKGSLKFLEDQQIFARDNYNAVMKQFENGLATSLDVM
                     DANTLLLTADKNVVDALYNYQLASLTVKKSSGTLLQFVRDGDPTVKRSSGALWQLTDV
                     GDLRAKKSGGRLLLFFSIDES"
     gene            19618..20742
                     /locus_tag="PLF77_05380"
     CDS             19618..20742
                     /locus_tag="PLF77_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPC08322.1"
                     /translation="MHLKNLVSFIEKKGSRRNTVKFIFLISLTAIIAFSLMSCKKEKI
                     VEKEVLTNVRVSTSVKKNIRPYIETTGTLTADEEVTVSSEVDGIVRSVRVEEGSAVGK
                     GTLLAEVNDIDYALDEKRAQAALKQAQANLTNVKAEFQRKEALFKEELITQQQFDDIS
                     TRVALAEADLAKAKATLDTARERLARTKIYSPLRAMVKEKRVSVGDYVRTSMPLFQLI
                     KVDRLKLNFTVGEKELADLKTSQEVDFTVDSFPGRKFKGTVHLLYPHLEERTRTLQAE
                     AVVGNAENLLKPGLFARATIYTGALREVVVAPLTSLLYDDATVSLFVVEGNTARSRTV
                     KTGRKYGEDIEIIEGLNEKEQVVVVGQNNLSDGVKVHVAR"
     gene            20729..>23721
                     /locus_tag="PLF77_05385"
     CDS             20729..>23721
                     /locus_tag="PLF77_05385"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPC08323.1"
                     /translation="MWLADTSVKRPVFATMVIMALVVLGIVSYPSIGVDLFPKVDFPI
                     VTITTTLKGASPDVMDVDVADRIEGAVNTINGVKTITSTSTESVSRTIVEFVLERDID
                     QAVQDVREKVSTIRSKLPDDIEEPRIAKVDPDSQPILYMNLTGEKSIRDLSTYVDEVL
                     KEQLQRINEVGDVTFNGLRLRQVRIWLDAAKMRAYEVSPSDVVVALKRENVELPGGRI
                     ETQTKEYTVRIKGEFPSIPDFNDLIVSYYKGAPVKIKDIGRAEDGTEEKRSLARFNGI
                     PAVGIGIQKQSGTNTVAVAARVKKEVERINQTLPPGMKINIAMDQSVFIVRSIDEVQK
                     HLILGSLFAVLAVFLFLKNIRTTLISAVALPVSVISTFALINAFGFTFNNMTMLALSL
                     SVGLLIDDAIIVIENIYRHVEEGMGPVEAAKFATSEIGLAVMATTLAIVAIFLPVAFM
                     KGLIGRFFLQFALTIVFAVLVSLLVSFTLTPMMASIFLKPHNAPPPKGNVLTRWSFWK
                     KAGDFLEKNYKRLETFYGRVLKFSLRHRAAMLFATLMVFLFSIFIMIFSLGKEFLPPE
                     DQGNFIIRLEAPIDYSVEQVEKYFGPAEKMVRDIPEIKSVFYVQGYQGYATRGIMIVG
                     LIPKADREKSQEDIKKIVRMNLKQIPGLKVSAEDVSLVGGGIRQVPIQYSIRGQDLNE
                     LQKYARQITTEFAKRPGIVDVDTSLEAGKPEFKVYIDRDKAADLGVDVATIAEAINLL
                     VGGELDIARYKDEKKGKRYDIRVRLNAEDRESSEAMQKIYVRARDGKLVELSNVVRIA
                     EGTGPSVINRADRQRAITIFASLEGKPLGEARNELDEIAGRILPADYVPKYYGMAEVM
                     QESFIFLLFALILGIIMAYMILASQFESFIHPATVLLSMPLSFIGAFGALFLTGKTLN
                     IFSLIGLILLMGLVKKNAILLVDYTNVLRERGMSRRDAILQAGPVRMRPILMTTFAMI
                     FGMLPIAFAVGEGAETRAPMGIAVIG"
BASE COUNT         6014 a         6015 c         6024 g         5468 t
ORIGIN      
        1 ttttcttctg aagggcaagc gcactcatca actggccttc ggaaacaatg ctgtcctcga
       61 ttaagatttc acccagcttt tttcttttat tcataatgtt tgagatatct gattaagttg
      121 cattatagca cgtctttaaa atattaaaaa tgaataaaat tatacgattt tccggcgcat
      181 ggctttgacg gatatgtcgt ctggaatgat caaaggctgt tttcgaacat cgatatgcaa
      241 aatccgtatg gacagatccc ccgcagcaaa accgcaataa ggcaaattgg tcttcaagac
      301 cggacgcatt gccttccaga aacttcacga tccctgcggg tttcttcgtt tctgaaaaac
      361 agcgggcgtt tgaaatgtac ttaatgtaaa cctattgtaa aaaacccgaa agcgcttctt
      421 tttctgaagc ttttagcgtc gggatagctc aatgaaaatg cctgtttccc ttccgggtat
      481 ttcttatcgt gaatacccct ggactcaaat tcgatgcccg gttatgaaat ttgttccccg
      541 tggaaattgg acagacctgt caggatgtta ttgagggcat ttcatccttc gcttaaatgt
      601 gcgaagatat tgacgaatct agaatttcag aaggttattt ccactccatg ctctcttgat
      661 tatgatattt ttttattcca tgacaaaatt tttattgact ttacccatca atcatactat
      721 tgaccgaaca ttcaattcaa atgattaatt tgaggatatc gcgttttttg tgaaagatta
      781 ttcataaaga gatcgtttgc gtgctcttta tagaatatat ttactaagtt gttatgcaat
      841 aataccaagc agtacgttgt ttcgcgttca tgatcaagcg gtacgttgcc tcctatctga
      901 agagacccgg cattcaacca gagattagta aaaatctaaa ccagattcgt ccatgaaaat
      961 ctgtccggat aacggatcgg atttgacgac gaaaagtgtc agccgatagg tgtcatctat
     1021 tggcattcaa actctttaga ataactgcat cattaagagg aggaatacac atgaaaagca
     1081 tgtctgtttc gtcagccagt gagcagaggg ggcatcaaag cgattcaacg gaccccctac
     1141 cggtaaacgc tcaggatttt ttcattcaga gccacaaggg attcaattac tacctgatcg
     1201 atttgaatac agccgaatgg aatcgtctcg gagatatcat tcggggatgt gaagcggttg
     1261 aaaatgattg ctggaaggcg caggaggaat tctcaaccta tctgcggaaa tgcgatgttc
     1321 ttagctatgt ggaaaaaaac ggccggatcg tcgcctttga tgccgtgtcc attctcaaca
     1381 gcggccccca ttgcgtgtac aataatgaag aaacgatggt gctcaaagca ttccggggaa
     1441 gagaccttgc tcaacgtctg gtcattatga cggtggaatg gcacctcacg aaagccgctt
     1501 caatcaagaa caaagattat ttcattttta cgtccatctc ggctaatccc gcagtggtca
     1561 accggtattt caataaatca tggctgcggg tactgtttga ctcctctttc tccccgtcgc
     1621 ccgcattaat cgctctgaag gaagagtatt gccgtaaata cgggatcgga cttgtggatc
     1681 agagctatcc tttctgcctg aagaacatgt ttcccggctc aaacggtttt gatcccaatg
     1741 atccgaagta tcaattctcg ccccatgtcc gggcaaacat gccggcggat ttcgaacata
     1801 tggacagggg cgacgcattt gcctttatgc tgatgatccc catgaaaatc gtacgactgg
     1861 tcaccttcgt tctgatgatc aaatgcttcg gcaaggaata cttttccagc aggggtgtcg
     1921 gactgttcag cagcagaaga ccccttatcc cgtcttttac cgaccggcgg gtcccgggtc
     1981 tgtccggaga acctttttca gtcaaaactg cggatcacgg ataaaatgat ttgcagaaga
     2041 atatacgata ttcaacaata aacgtaagag aattaacgtt tgaaacaacc agtaaatgag
     2101 gaggctccaa tgattaagtt acagaagggt tatttaaaaa tttcctgcgt cttgatgtgc
     2161 ctattggccc ttatcttcgc gacgcggaca tgggcctgtg atgtggccgt tgtctccggc
     2221 aaggtcacca cagacggcaa accgctgatc tggaaaaact ttgacaactc gtcaagcgac
     2281 cgccagcagg ttgcctactt ccccgccaaa aagtccggtc ccggcggata tttaatggtc
     2341 taccggtttg aaaacgggat gaaactgctg aacaacggct cggcgatcac tccttccggc
     2401 ggcgtcaatg aatcaggttt tgccattgcc ggcacgtccg tgtaccagga ttataatctt
     2461 atggcggaac cggtgaacat caataccgtt cttatggagg aagccctggc gacatgtacc
     2521 acgctcgcgg agtttgaatc actgctcaaa agatggcctt actatcactg gggcaccgtt
     2581 atcagcgcca acttcgttgt catcgacgcc aaaggcggag ccgcactgta tgaatgcttc
     2641 accggccatc taaacaccgg cctcaacctg atgcagttca gaaagaacga tgccaacacg
     2701 ggcaagatca ccaatcagta tggatggacg ctcaaacggg ctcagagcaa cttcatcgga
     2761 ttccacatta tgaccaatta caattactac attccctgga acgtgggaca ggatcgcaag
     2821 tatagagccg aagccctcct gacccagttg gcaacagaga agcggatcaa ttaccgcacc
     2881 gttatgcgag aggtggccaa ggacgttgtc ggcaagcagg tccagacgga tctttccagc
     2941 gagaccaatt acagcacaac ctattgcctc agccgcaatg ccacccgtag ctcgatcgtc
     3001 gtcaggggcg ttgcatccgg agacaacccg cgcctggcca ccatgtgggt caacctcgga
     3061 gagccctctg taggtgtttt cgtcccgttt tttgccgccg ccaggggaac atcggaactg
     3121 gcccgggtgg atacgtatgc caacggtgcc tggagagatt acgatgattc ctgtctgctc
     3181 aatcaggcca tcagcgcgag ggaaacctac ggcagtctca tttattcatc gaatgaagga
     3241 gatgtcatct ggggcatgta cgataactac atcaataagg tcgaactggc caaggttcag
     3301 cagtggacat tccagatcga ggatacgatc attgataatg ccgaatcgtt cctgtcttct
     3361 ctccaaagca acccggcaca gattaccgct ttcaatctaa aggattttgc ggattactgc
     3421 gcggattatg cttatgaaaa cttcgagacc ggctctgcgg atgcctttcc ctggcaatac
     3481 taatatgatg atggacgagg ggcactgcga agatccctcc cgtggtcata tttttgaaag
     3541 agtcccctga agacgtattc gatgacggaa ttagatggac aagacagcca agatatcaaa
     3601 ctctcaagcg gtgatggggg atatggattt tccacatgag gaaagggcac gccctttcct
     3661 catgaatacc aaactatgga aacagctact ccctctggcc gtctccggcg caatcctctt
     3721 ttatttgttc cggcacattg atatccgcat gtgtttcaat gccatgctga ccgcgaatat
     3781 gtccctttat attccctctt tgattgccct cattctgatc acctttcttc tggacacaca
     3841 gaacctggct gagaccctga agcattttca ttaccccctt tcctggaaaa atgccttcac
     3901 tctgcgcggc gtcacctatc tcatcatgac gatcgactac agtgtgggca tgggagttct
     3961 gatctactat ctgaaaaacc atctcggcat cccggtcatg cgatcaacgg gactgatgac
     4021 ctttttcaac ggcatcacac aaaaggctct ggtttacatg gccatcatcg gcttggtgat
     4081 tctttctccg gcctcgggat tgctgcgtaa tttgctgatc ttcttcatcg gctttgccct
     4141 gctggatatt ctttttatta tcgtgttgaa aaaactgccg tcccgcggcc tggccctcaa
     4201 aattaaaagc atgaaccttc tgaaggtctt tcacgaggct tcctggcgaa cctacggtgt
     4261 cctcatcttc tggcgcattc tctattatgc ctgctttgtc gccttctttt atgttgcggt
     4321 cagggcattc aacatgcaaa tcccgctggt tgcgcttatc gcttatgtcc cgatcattct
     4381 cctggtcatc agtctgccca tcgctccggg cggattcggc acggcacagg cgacgatgct
     4441 ccttctcttc aaggattacg ggacgcccct gaatatcatg gctttcggac tgatctacac
     4501 cacctccatc ctggtgttgc gttacctgat cggcctgctc tatgcgaaac atatcaaggg
     4561 tcttcctgcg ggagaggaca gcacaagcga acaaaggaat tgaggatgat cggcaggcct
     4621 gcaccatatc cccttgatca atcagtgccc cgtcttcaaa aggaacctag aggaaacctg
     4681 tatctcatca ccaccctccg taaaagaaag aatggccgcg accgaacgat gtcccgggca
     4741 ttgcatctgg cggttgtgat actggtcacc gccttcagta ttcaaaccgc tgtcgcacag
     4801 gagcattttt cacatgctct cggtcatttt gaaacactat caacggcgca gggcattctc
     4861 attgcggcac cgcatggaac ctatgacatc cgcacggatg tcctggccaa agatgtggcc
     4921 cggacgctcg gtgcaggcta tgtcatcgcc ggaggttttg cgtcgccatc ttcgcgcatc
     4981 aacgtcaacc gcccctcgga gggtgcagga catacgtgcg aaaaagaacg gcgtaccgaa
     5041 agggcgaggg atgtttacga ccaatacgcc cgtttgattg acaagatccg tgacggcaag
     5101 ccgttgcggc tttatgtcga aattcacgga cacaccagca tcccttttct caaccggctt
     5161 gaaatcgcca ccacaggaat cacaacggaa gaagcccggc gcctgaaaga ccgctttccc
     5221 gctttccttg cgcaggcaaa ggcagcctat ccggcctacc ccgatctgga gctgcgcata
     5281 gagcccctgg ataagatcta cttcggagca cgctgcaaca aaacgaccgg atatctctcc
     5341 acacggaaca tggaacgtgc gcttcacatg gagatccccc gcccggcgcg aactcctgag
     5401 gcgttgaaag ccaccgccgc gcttgtcagt tccattgtgc gcgccaccat gaccatttca
     5461 gaatcagcct ccggtccggg acccaagagg gcaacttccg atgcagacaa gaaatcgtca
     5521 gggggaccgg cgcgatgacg aaacggtata ctcggaaaat gacgtttaat gaccgggcat
     5581 ttgtggtgct caatcaaatc agtcccccca cggtcaacca atttattttc gacggtgatg
     5641 ggacgctcga tccggaaaaa tggcgcaggg cggcggatgc ggcttccgag gccaatcccg
     5701 gtacccgtct ggtcctcagg ggtcacctga gctggagccg atgggtcgac tcgggaataa
     5761 acatgcgcgt ccgcgaagtg gacggcagga actgggacgg acggggcccg gagaatgaac
     5821 cattactcga agagtctctc ccctatcggg gaaaccccac ctgcgagatt gttctgatcc
     5881 agggtccgac cccgagagtc gcattccgct cccatcacgc cgtgtgtgac ggacggggca
     5941 cgctgttctg ggccgaggat gttttccgga ccctgcgcgg cgaagctgtc gtcggttcaa
     6001 cctcaaacat cacggatgtg gagcttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnncctg cgcggcgaag ctgtcgtcgg ttcaacctca aacatcacgg atgtggagct
     6181 ttgtaagagt tttcagaatc ggtaccggac gcctttcccc gcagagcata tcgcccccgc
     6241 cggacgggca aaaggcaatg acaccggcgt gacctggaga cgttgttccc tcaagggcaa
     6301 gatgccccat ctgctggcgc aatgcgcccg tctggcagcg gaagaagcct ggcgacatgg
     6361 cgaaggggtt gtccgtttcg gtatccccgt ggatttgcga catcgcagac cggaactgcg
     6421 cagtacagcc aatctatctt ttgccctgta tacggaggtc aaaaaggaaa cgagcccgga
     6481 cgacatcgcc cgggacatcg ctttgcaaat cagtcgatgc gatgaatgca gactgaccaa
     6541 gggagacgag cgtctggaat acatgcccct gtggatgatg cgatacaagg caaaaaatat
     6601 cattcgcgat cggcaccggc gcggattata cagtctttcg ggcatcctgt ccaacctggg
     6661 ccggattaat cttaaccggt tttgcggcgg ggggttcaca gcacgggcat tctggattat
     6721 tccgccggct gctgaatact atccgttttt tatggtcatg acgggctatg aaggggggtc
     6781 agatctgacg atttccatgc ccaaagttct ggccagtgaa ggccggcttg aaaacaccct
     6841 gaagagaatc gttcatggac tggaagggac aaactaaagg gaggatacga tatgacttac
     6901 tcgcggcaac tgagttccac cgagcgggca tatgtggtgt acaatgaatg ctgtccgccg
     6961 tttctcaacc agttattttg ggaaggaacc ggatatttcg acctgcccaa atggcgggaa
     7021 gccgtcaaaa tcgcatccga ggccaatccg ggtagtcgcg tcgttgtcaa gggtatcacg
     7081 gcctttgccc gtctcgtcga taccggaaaa actccgccgg tgatcgaggt ggacggatcc
     7141 cattgggacg gtcttggtcc ggaaggcgct ccggccaagc tctcagaaac cctgaatcct
     7201 tttgaaggcc ctacatccga agttgttctg atccacggca acccgttgcg cgttgtcttt
     7261 cgcacgatgc atgccaccat ggacggccgg ggcaccgtga cctgggtgga agatattttc
     7321 cgcggtctgc gcggcgaagc tcccctggga tcggattcgc gtatcactga atttgagatt
     7381 gcggcaacct ttcagaagca aggacgcaaa gggccggttc cggaatacat cgctcccacc
     7441 ggtatgcctc agggcgatca gcccggcgtg atctggcgcc gacggcgcta ccccggacgc
     7501 taccacaatg tcctgggcca ggtggccact ctcctggccg gaaaagcccg gcagtatcag
     7561 gaaggtaagg tgcgcctggc tattccggtg gatatgcgcg ccagggttga gggcctgcgt
     7621 tcgacaggga atttgaccaa tctgatttac gtggaaatca atccctccac aacaccggct
     7681 gacgtgagtc aggacatcaa gcgtcagctg gaagcgcggc atgacggcat gatctactgg
     7741 ggcgaccggc cgattcgctt tctaccgctc tggttgctca agaaactgat taaaagcaat
     7801 atcgccacca agaaccgcac gggccgctac cccaactccg gggtcctcac caatatggga
     7861 cggattccgg ttttcagcgg agggggcttt gaaggaaaat atacctgggg cattcccccg
     7921 ggccaggaat actcccccat ttttatcggc atgtgcaaca gtgatgatga tatgacgatg
     7981 atctattcca tgccgaacgt tctggccggc ggcggccgtc tggaaacact gatggaccat
     8041 attcaacagg gactggttgc ctcgaaggcg gaccggaaga aaggatgata cgacatcatg
     8101 tggatttctg atacctcaat caagcggcct gttctcgcca ccatgttcat tctcgctctc
     8161 gtcgttctgg gcctggtcag ctacccgtca atcggcgtcg acctctttcc caaggtggaa
     8221 tttcccctcg tcaacatcac gaccggtctg gccggcgcaa gccccgagat catggatgtc
     8281 gatgtgacgg acaaaatcga agaggcggtc agttcgatta acggggtgaa gacgatccac
     8341 tcgctgagta ttgacggggt gtccagtgtc atcgtggagt tcgaattgga gcgggacatc
     8401 gatcttgccg tccaggatgt gcgcgagcag atctccgccg tccgttcgca gcttccggat
     8461 gacatcatcg agccggtcat ccagaaggtc aacatcgatg ctagccctgt cctgtggctg
     8521 gccctgaccg gggacaagtc tcaacgcgaa ctctcaacct atgtggatga aagcctcaag
     8581 gagaagttac aaaaaatcca gggtgtcggc gccctctccc tnnnnnnnnn nnnnnnnnnn
     8641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn ntaccgccac atcgaagacg ggatgtctcc gagagaggcc
     8761 gcgtcctttg ccacggacga gatcggtccc gcggtcatgg ccaccacgct cgccattgtg
     8821 gtcatattcc tgcctgtcgc tttcatgaaa ggcatcatcg gacgtttctt catgcagttt
     8881 tcgctgaccg tcgtcttttc catcctcgtt tccctgctcg tgtcgctcac cctcacgccc
     8941 atgctctcgt cccttcttct ccacccgcgg aaaaaagaat ccttggacag tgaacgcccg
     9001 ggcacggtga tggcgcggat cgggcagacg ctggagcgcg gatataaaaa aattgaagag
     9061 gcctaccgcc ccatcctgac attcagtctc gaccatcgcc gggctgtgct gatgagcgcc
     9121 ctcatcctct ttattttcag cctctggatt acccggttca tcggcaagga attcaaaccg
     9181 ggagaagacc agagccagtt tatgatccgg atggaatcgc ccatcgatta ttccgtggaa
     9241 cacgccgggg cgatgttcag tcaagccgaa aagattgtcc ggaacagaac cgaggtgaca
     9301 tcggtcttct atggtcaggg agcgggtcag gggcgactgt cacaaatcaa tcaggcgttc
     9361 atccaaacca gcctgaaacc gcggtcggat agagataaat cccagtcaga gattatggcg
     9421 gaattgcgga aggaatttgc cgcgatcccc gggctgaaaa ccaccatcga agaggtcgcc
     9481 gttctcggag gtggaattcg gaatgtcccc gttcagtatt ccatcagcgg cggatccatc
     9541 gacgagctca gaaaatatgc caagcaaatc gtcggtgaac tttccaaaat aaaggggctc
     9601 gtggatatcg acacctccat cgaggcaggg aaaccggagt tgagtgtcta cattgaccgg
     9661 gaaaaggcaa cgaatatggg agtcagcgtg gcagccgtca ccgaagcggt gaatctcctc
     9721 atgtccggcg aggtcgaggt cacgaaattc aaagatacag caaaggggag acgatacgat
     9781 gtccggatga ggctggacgc caaagacagg atgagcccgt ccgatatcgg cagactatat
     9841 gtccgggccg tgaatggtga gttggtcaga ttaagtgaca tcgtcaccgt cgaggagggg
     9901 ggcggtccga gcagcatttc gcgcaaagac cggcagcgca cgatctggat attcgccaat
     9961 ctccagggca agccactggg cgaggccatg cgggagattg acgccatatc cgccaggatt
    10021 ctgccggccg gatacgttgc cggttactcc ggagaggccg aagaaatggg caagtccttc
    10081 aagtacctta tgtttgccct gctgctggga attttgctgg cctatatggt gctggcctcg
    10141 cagtttgaaa gtcttcttca tcccgtgacc attctcctgt ccatgcccct gtcgtttatc
    10201 ggcgcttttg cggctctcct gatcttcggt aaatcgctga gcatcgtgag cttcatcggc
    10261 ctggtactcc tcatgggtct tgtcaagaag aatgctatcc tactggtcga ttttacgaac
    10321 accctgcgtg agcggggcct gtcacggcgg gaggccatcc ttcaggcagg accggtccgg
    10381 ctgcgcccca tcctgatgac cacgtttgcc atggtgttcg gtatgctgcc tgtcgccctc
    10441 ggtatcggag aaggcgccga tctgcgcgcg cccatgggca ttacgctgat cggagggctt
    10501 ctcacttctc tgtttttgac cctggccgtt gtcccggcgg cctatgacat attcgacgac
    10561 tggaaagaaa atatgatccg ccggttaggc agccggggga tcagtccctt gatccggcct
    10621 tttggaaaga ataaaaaggg gaataacaat aactaggagg tatccgttat gatgaagacg
    10681 gccatattgc tgtatcactt tttcaaatcc ttaatcgaaa tccacgtcag cgcccgtctc
    10741 ccctatggac tccagaggaa actatgcgaa agacgcttga ttcgctccat ccggcatgct
    10801 tatgaccatg tccccttcta tcgccagaaa tacgatgcgg cgggagtgga cattacatct
    10861 atccggtccg ccgatgacat caaacggctc cctttcgtca ccaaggaaga aatccggcag
    10921 aattttcccg acgggatcgt ggcacgcggc gtcaacagag aggcgtgccg ctattcggcc
    10981 accaccggat ccacaggcag gtccctgccg ttcatcttca ctcaggcgac ctatgccttc
    11041 tacgtcgccc tgggggtccg catgtacaca atgatcggat accgcccatg gcacaaaatg
    11101 gcctacatca aatataccga cctgaactat acccgtctgg gacccttctt ccgggccgtc
    11161 catatcaaat cgacgattcc cgtcgaagaa cagatcgcgc agctcaggaa ggcgggggcc
    11221 gacctcatca tcggttatgc ctctctggtg tacgagatcg cgagaaacgc cacccctgat
    11281 gacctgaagg atatccggcc caagttcatc ggaatcaact cggagctttc gactgaggcc
    11341 cagcggacct tcatctccga aaccttcggc tgtcccgtct acgatgaata ctcgacggaa
    11401 gagacctgga tgatcgcctc ccagtgccgg tacaacaact accatatctt tacggacaat
    11461 gtgtgggtgg aattcatcga ctcgaaaggt caggaggttg ccccgggaga catgggagaa
    11521 atcgtgctga cgactacccg cagccctgcc atgccgttca ttcgctaccg tatcggcgac
    11581 atgggaagat tcagcaacgc tgaatgtccc tgcggcatgg ggttcccgct actccaatcc
    11641 ttcgaaggca gggccgacga ttgctttatt cttccgagcg ggaaattcgt ctcctcgctg
    11701 aaacttctga acaccttcac catgtatatc aaaaagtacc ttcatctgct ggaggagttc
    11761 aaggtgacac aggtgcatag cgatctaatc gtcatcaaaa tcgtcaaagg gaatcagtat
    11821 gacagcgccc atctcgaaga actgctggcc gatttgcgtc gtctacttga cgattccgtc
    11881 tcgatcaaaa ccgaattcgt cgatgttatc gagaaaacgg acggcatcaa gcgcaaagcc
    11941 attgaatccc ttgtcagtca gaagcagacc ggttctcctt cctgacggat cagcgggatc
    12001 gctatccatt ttccgtttaa tcactggcgc catattcgga tcaaatcaag cagaatcgtt
    12061 gccgcctcgg tgacgggtgc cttcttgcgt ggataaggtt ttcgcaccct ggtattattg
    12121 tgaatcgtgt tggaagacaa tgccgtttga gatggacagc attgatatat ggcacaccgg
    12181 gcgtggtcaa atgacaatcc catggcacat ccgggacttc aacagaggaa tcgtcactga
    12241 aacagtttga cgaatccgtt caatgcggcc acaaaccgga tccttccgtt gaggaaacaa
    12301 tccggcggaa agggtttatt gttcgttgga ctgatttcaa acaaaacgtt accggttcgt
    12361 ttttaaatca gcggggttca tccagtttac cctcccagag aaaatgtttt gtttcgctga
    12421 caatcacctt attcagaaca agcagtgaaa tgagattcgg tactgccatc aatccgttgg
    12481 cgatatctgc aaaggtccat acaatgggaa gactcagtac ggaaccgagc atcacagcac
    12541 ctacccacag atacctgtaa ggccgaacgg ctttggttcc cagaagatac tccgcggctt
    12601 tttcaccgta gtaggaccag cccagaatgg ttgaaaaaac aaacgtcagc aggccgatgg
    12661 aaagcacaaa cggcccgaca accggcaaag ccgaaaaggc cgccttggtg agcgcggcgc
    12721 ctttgtagcc gtgaatccat tcgccggaat tgaccagaac cagaccggtt attgcgcata
    12781 caactaccgt gtcccagaag gttgccgatg acgaaaccag cgcctgacga atggcatttt
    12841 tggtctgcgc cgccgccgct acaatcggcg cgctgcccat gccggattca ttggaaaaca
    12901 ggccgcgcgc gacgccgtag cgaattgcct ctttcatgcc cgcaccgaga aacccgccga
    12961 taacggcatg tccggaaaaa gcgctttgaa aaatcagttt gaacgtttcc ggtatcgttt
    13021 cataatgcat gcccaataaa atcaagcagc cgaagacata aaacaccgcc ataaaaggca
    13081 ccagtttttc gcagaccctc gcgatggatt taatgccgcc gaggataaca accgccgtca
    13141 gcaccgtcat cgtcagtccg gttatccacg gtgaaatatt gaaggtttcc tgcaccagcg
    13201 acgcaatcga attggcctgt accatgttgc cgatcccgaa tgccgccact gcggtcagcg
    13261 cagcgaaaat caatcccagc caccgggcgt tcataccgcg ctccagaaca tacatggggc
    13321 ctccggccat taagccctct ttgttggtga tgcggtattt cagcgataaa accgcctcgc
    13381 cgtattttgt ggcaatgccg aagacgccgg tcagccacat ccacagcacg gcgccggggc
    13441 ctccggcggc aatcgccgtc gctacaccga cgatattgcc ggtgccgatc gtggccgcca
    13501 gggccgtggt cagcgcgccg aactggctga tgtccccctc cccttcgcgc tttcgtgaaa
    13561 tggacatttt gatggctttg aacatatagc gttgaatgaa tttgagacgg aacgtcagaa
    13621 agatatgcgt tccgaccaga agcacaatca gaggccagcc ccagaggata tcgcttattt
    13681 gctgcagaac agattctatg agttgcataa tgtctccata aatatttttg aacagtctgt
    13741 tgagtatgaa ccatcaactt ttgattttcg atgcttcatc gccgccatca aacatatccg
    13801 acaagaaatg accaaaagga tgataagggg agatgatgtt tcacgaaacc gcattcggga
    13861 aacctgtttt tttccattgt ttataaaaga agggtgggtg cgctgtcaac ggaaaagaaa
    13921 attattttca tgctaaaacc aaccgcaaat agttgacgga ttccgtcata aagcataatt
    13981 tcaccttgat gaatcgggag aaagaacatc tggcaacgtt gcggggcatc ttctacagga
    14041 aagaacagaa aaaaatttta tgctcgggca tgctgccccg atgagatgga tgtgtttctg
    14101 aacatctttt tttacgacaa atttataaaa aagctacggt agctttaagc aaaaaaactt
    14161 gagtgcaatg cccggtcatg atattaatta aaaaaatcaa caaagggaca ttccattgaa
    14221 gcttgcgact tataatgtca attccattcg ttcccgtatg cacatcattg ttccgtggct
    14281 gaaagctcac agtccggact ttttctgcat gcaggaaacg aaggtcgccg atacggtttt
    14341 ccccgcggca gagtttgaag ccctggggta tcatgttgtt ttcaaaggcg acaagcagta
    14401 caaaggcgtc gcgattgtct caaaacaaaa accgcagaag gtgatttacg gttttgacag
    14461 cgagccgtcc gatcccgacc gtatgatcat tgccgtattc aacgatctga cgatcatcaa
    14521 tacttacgtt ccccagggtc aggaaatgca aagttcccaa tttacataca agctggaatg
    14581 gcttgcccgt tttaaaaaat atctgcaaaa aaatttccgg ccgcaaaatg aactgatctg
    14641 gtgcggcgat ctcaatatcg cgcctgaacg gatcgacgtt catgatccga aaagactgct
    14701 gggccatgtc tgtttcaatc cggacgtctg gacagctctc aaggacatca aatcatgggg
    14761 attgacggat gtgtttcgca aacaccatcc gggaatcgcg ggtcaatata cttttttcga
    14821 ttaccgtata aaggattcgg tcaaacgcgg actcggctgg cgggttgatc acatcctggc
    14881 aacaaaagca ctggccgaaa ggtcaaagag cagcgccatc gatctggact cacgactggc
    14941 ggaaaaacca tccgatcacc tgattcttta cgcgcagtgg taaggggccg caatggataa
    15001 aaaagaaaat attgaacgca tcaacacaaa cattgccgac cgttttgaca aagtcgaaaa
    15061 aaaattgtcc ggagccggaa gcattgccga actttttgaa atgctgctta cgcagatcga
    15121 agcggaattt caggtgccat tcatctggat cagcgtggcc gataccataa aaacgggccg
    15181 ggtcatcgac gccttcaaat catcccgtat tttaaaggac aggctcaatg tcatcaaagc
    15241 cgatcaattt cgtgaaatct tcttttccgg cctgcaaccg attctggtca ataaaaactt
    15301 aaggccctac tataggttat ttccgccaaa tcagaagtat ttcataaaat cgctggcgat
    15361 ggttccgttc aaaatcaacg gagaagttgc cggcagctgg aacaacggcg atgccattca
    15421 gagccgttac tctccggata tggagacaag tcttctgcaa aaactttccc gacggctctc
    15481 cgcccggctg gatgaacttc cttaaaacgc atgatacaaa caggcgaacc ccgccgaact
    15541 gcgcggggtt cctgaagggt atatctttga aggggaaaag gggttagttg atcaagtttt
    15601 taagggcatc aaccaccggc gccgtaaaaa tggacaccag taaagcgtaa agcgcaaaag
    15661 aaccgatggc ttcgctcata tacaacttgg tgtactttct tttcagggaa atgatgatca
    15721 aaccgccaac gattaaacaa ccaaggtgaa agaacggaaa attggcatca ccggtaacgc
    15781 tgtactgagc ataactgaaa gaggtggtta ccagaactac aaaagcggtc aagagggtgg
    15841 tttgcattaa ttttctcata gctctttttc tccttaattc gatgatcttg aaaactcttg
    15901 gtcattttta atgaaataag tatgccaaaa tgcataaaag gcgttattat tttataaact
    15961 attaaaatat aagaataata atttttttcg ctaggggaaa aaaatttcct ggaatagcta
    16021 tgatgtttaa agaaacgtta aatttcagaa aataatagaa agaagttttt aatattttcc
    16081 cgatgaatta attgcattat ccgaaatatt tggcttgaca aatcgtcaat atgctataaa
    16141 aaagtgaacc acaattcaaa atttgaatga ttattcacag catgagtttt tgcgatggaa
    16201 agactggaaa gaaaagaacg cgaattcaac atgcgccggg ctgagattct ggaaaaagcg
    16261 gaaaaaatat tttccgtcaa ggggtaccat aatgtcacca tggcggaaat cgccagcgcc
    16321 tccggatttt ccacgggatc tctctaccaa ttttttgagg gcaaggaaaa tctttactca
    16381 tccatgatca gcgaaaaact caatttgatg tatgaagaaa caaaaaaggc aaccgatcgg
    16441 gcggataacg tactcgataa aatcgagatg ctggttgatg cccagctgcg gtttctggaa
    16501 gagaatgccg atttctgccg tttattcatc aagggcgaga gcaccgctct gtcggaagcc
    16561 atgacatcca tgcgggaaaa aatcatcaag gattatttta aatatatcgg ttttattgaa
    16621 aacttgctga aagaagggat tcagcaaaaa ctgttaaaaa acctgccggc cggcgacatg
    16681 gctcaatccc tggtttattt gatcagggcg gtatccatgg aatggatgtt atcattgaaa
    16741 aacgagttcc cctattccaa aaaggggttc attctggaca ttttttttaa tggggttaaa
    16801 aatcatgatc ggtaaaaaaa tccggatttt gaccatagtc gtgatgattg ttttttcgtt
    16861 acgtctgagc ttcgcccagg aggttctgtc gctggaagcc agtattgacc tggctttaaa
    16921 aaacagcaac atcctgaata tcgcgaaaga aggattgaac agcgccacgg ctcagaaacg
    16981 ggaggccttg accggctttc tacccaagtt cagcacggcc tacagttact cccgtttaaa
    17041 cgaagcgcct ttttccagat tccagggact gcctccggga ccgatgtatg acttaaacgg
    17101 catggaaact cccgtgggaa cgaaagacaa ttacaactgg atcatcgaag ccagacaacc
    17161 gctttttgcc ggcggcggaa ttctggccaa ttatcaggcc agcagcatcg gcgaagaggt
    17221 tgcccgcctg gaggaaacgg cgcgcattca ggacgtggtc cgggacgtta aagcggctta
    17281 ctttaatatt ctgcgcgcgc agaaaatcaa cgaggcaacc gctcaatcgg tggaaatgct
    17341 caaagcgcat cgcgatgtcg ccgaaaacta tttcaaggta ggactgattc ccaaaaatga
    17401 cctgcttttc gccgacgtgg aactggccaa cggaagacag gccctggtcc ggacacaaaa
    17461 tgcggtggag ctggccaaat cgcgtttgaa tacgctgctg aaacgcgata tttccgcccc
    17521 cgtggacgtc atggatattc tggcttacca accgctgaag cagacgtttg aagaatgcct
    17581 cgaaacggcc cgccggctga gaccggaaat gaaaatatcc tcgctcaaag ttcagcaggc
    17641 cggcaaactg gtgcgcatgt cgcaaagcga atattatccc gctttaagtc tggtcggtaa
    17701 ttatacgcga ttcggcgacg aggcgtccgt tcacggcagc gattttaaag atgcggagag
    17761 ctggtacatt atggcggtcg ccaactggaa tttctgggaa tggggcagaa caaaattcag
    17821 ggtcgatgcc agcaaagcca gagaaaacca ggcttcggaa tcctccaaag aattgatcga
    17881 ccagatcgct ctggaaatca aaaacgctta tctgttgctg ctggaggcgc aaaatcagat
    17941 ctccgtctcg gaaacgttga tcaaacaagc tgaggaaaat ttccggatat ctgaagaacg
    18001 atacaaggaa agagtcgcca cgtccacgga agtactcgat gcgcagacgc ttttaacccg
    18061 tgccaaatca gaatacgcca gtgccctggg cgattacaat atcagtttgg cgcatctgca
    18121 acgggcgatg ggcaatatct ggccataatt tttttgcgaa aggaattcat gatgaaaaaa
    18181 agtttgctct gtatgatgac tgtgctggcg acggcgctgc tgtctgccgc aaacgccgta
    18241 gcgcaggaat acactctccg tgaaatttat cagaaggcgc ttcatacgtc cgaaaaaatg
    18301 gaaatcgccc gcgaaaacgt ctatgtcgcg cagatgagca aagacaaggc cctgtcgctt
    18361 ttaatccccc ggctgacggc ctacggaacc tacaaccggt ttaccgaaga caagtacaac
    18421 acggcgaata tcatgatcca gccggaagac tcggcagcgt ggggggtgcg cgccgatcag
    18481 actttctcgt tgagcgcccg cgagctggat gctttaagaa tagcgggcca gtccatttcc
    18541 aagagcgaat atgatctgga ttgggccaaa tcggattttc ttctggcggt cgcctcctct
    18601 ttttacgacg tactcaaagc ccaaaaagct ctggatatta cggtatccaa tctggaaaga
    18661 ctcaccaaat attaccatct ggtcgaaacg cggatgaagg tcggcgaact gaccaaaacc
    18721 gcgcttctgc gtgcccaggg agaattatcc ggcgccaaat cggactatct caaggcctcc
    18781 aatgccctga aactggcgcg cgccgccctg acgcgcctga cgggggtgga accggatttc
    18841 cggctgaagg aagaaaccgt atccctgtcg gaagcgtgtg acatccaggg cctgcgcaaa
    18901 tcggcatcgg atttgcgcac agatctgaaa agttacgata tgcagacgaa aatggccggg
    18961 cagcaggtca aatacgcccg cggcgccttc tggcccagtg tggggttgtt tgctatttat
    19021 aaccgggcgg accaggatcc tgccggctcg acatttaatg acgaaaacat ttacggcggc
    19081 atttcgctta ccttcccgtt ttttgaaggc ggcctgagaa tggctgaatt ccgggaagcc
    19141 agggctaaag agcgtcaggc ccgcctggct tacgacgatt taaagaagag cgtggatatt
    19201 gaagtggagg ccgcctgtct cgacctggac acattgaaag gttccctgaa atttctggaa
    19261 gatcaacaga tttttgcccg ggataattac aacgcggtga tgaaacaatt cgaaaacggt
    19321 ctggcaacca gtctcgatgt catggatgcc aacacactgc ttctgacagc agataagaat
    19381 gtggtcgatg ctttatataa ttaccagctt gcctctctga ccgtgaaaaa atcaagcgga
    19441 acgctactgc agtttgtcag agacggcgat ccgacggtca aaagatccag cggcgcatta
    19501 tggcagttga ccgacgtcgg cgatttaagg gcaaaaaaat cgggcggcag gttattgctg
    19561 tttttcagca tcgatgaatc gtagaaaaaa agacttaggg ccaggaggga aaaggttatg
    19621 catttaaaga atcttgtcag cttcattgaa aaaaaaggat cccgacgcaa cacagtcaag
    19681 tttatattcc tgatttcact tacagcgatc atcgcttttt cattgatgtc ctgcaagaaa
    19741 gaaaagatcg tggagaaaga ggtcttaacc aacgtccgtg tttcgacgtc ggtcaagaaa
    19801 aacatccggc cttatattga gacaacagga accttgacgg cggacgaaga agtcacggtc
    19861 agttcggaag tggacggcat tgtcagaagc gtcagggtgg aagaagggtc tgccgtgggc
    19921 aaaggcactc tgctggctga agtcaacgat attgattatg cgctggatga aaaaagagcg
    19981 caggcggctc tgaaacaggc ccaggccaat ctgaccaatg tcaaagccga atttcagaga
    20041 aaggaagctc tctttaagga agaactcatt acccagcagc aatttgatga catctccacc
    20101 cgggttgcgc tggcggaggc tgacctggcc aaagcaaaag cgactctgga tacggcccgg
    20161 gaaaggctgg cccgaacaaa gatatactcg cctctgcgtg caatggtgaa ggaaaaaaga
    20221 gtctccgtcg gggactatgt gcgtaccagt atgccgttat ttcagttaat caaggtcgac
    20281 cggctgaaat tgaatttcac cgtcggcgag aaagaactgg ccgatttaaa gaccagccag
    20341 gaagttgatt ttaccgttga ttcgtttccc ggcagaaaat tcaagggcac ggtacatctg
    20401 ctttaccccc atctggaaga acgaaccaga acgctgcagg cggaagcggt tgttggcaat
    20461 gccgagaatt tactgaaacc ggggttgttc gccagagcca ccatttacac gggagcgttg
    20521 cgcgaggttg ttgtcgctcc tctgacatcc ttgctgtatg acgacgccac cgtcagtctt
    20581 ttcgtcgtgg aaggcaacac ggcacgatcc cgaacggtta aaacaggccg gaaatacgga
    20641 gaagatattg aaatcatcga aggcctgaat gaaaaagaac aggttgtggt ggtcggtcag
    20701 aacaatttgt cggacggagt aaaggttcat gtggctcgct gatacatccg tcaaacgtcc
    20761 tgtttttgcc accatggtca tcatggctct ggtggtgctg ggcattgtga gttaccccag
    20821 catcggcgtc gatttattcc ccaaagtgga tttccccatt gtcaccatta ccactacgct
    20881 caaaggtgcc agtcccgatg tcatggatgt tgatgtggcc gacagaattg aaggggctgt
    20941 caacaccatc aacggcgtta agaccataac gtccaccagc acggaaagcg tttcgcgcac
    21001 catcgtggaa tttgtgctgg aaagagatat cgatcaggca gtccaggacg tccgtgaaaa
    21061 agtatccacc atccgcagca aattgccgga cgacatcgaa gaaccgcgca ttgccaaagt
    21121 cgatcccgac tcccagccga ttttatacat gaacctgacg ggcgagaagt cgatacgcga
    21181 tctttccacc tacgtcgatg aagtattgaa ggaacagctt cagcggatca atgaggtcgg
    21241 tgacgtgact tttaacgggt tgcggctcag acaggtgcgc atctggctgg atgccgcaaa
    21301 aatgcgggcg tacgaagttt cgccctccga cgtcgttgtc gccctgaaac gtgaaaatgt
    21361 cgaactgccg ggcggccgga tcgagacaca gaccaaggaa tataccgtcc ggatcaaagg
    21421 cgagtttccc agcattccgg actttaacga tctgatcgtc agttattaca aaggcgcgcc
    21481 ggtgaaaata aaagatatcg gcagagcgga agacggtacg gaggaaaaaa gatcgctggc
    21541 gcgttttaac ggcatcccgg ccgtgggaat cggaatccag aaacaatcgg gaaccaatac
    21601 ggtggccgtc gctgcgcggg tcaagaaaga agtggaaagg atcaatcaaa cattgcctcc
    21661 cggcatgaag atcaacattg ccatggatca atccgttttt atcgtccgat ccatcgatga
    21721 agtgcagaag catttaattc tcggcagtct ctttgccgtt ctggccgttt ttctttttct
    21781 caagaacata cgcacaaccc tgatcagcgc cgttgccctg ccggtctccg ttatttccac
    21841 ttttgcgtta atcaacgcat tcggctttac cttcaacaat atgacgatgc tggcgctgtc
    21901 gctttcagtg ggtcttctga tcgatgacgc cattattgtc atcgaaaata tttaccggca
    21961 tgtggaagaa ggaatgggtc ccgtggaagc agccaaattc gccacatcgg aaatcggtct
    22021 ggccgtcatg gccacgaccc tggcgattgt cgccattttc ctgccggtgg ccttcatgaa
    22081 aggcctgatc ggccggtttt tcctgcaatt cgccctgact atcgtctttg ccgttctggt
    22141 atccctgctc gtttccttta ccctgacgcc gatgatggcc tcgatttttc tcaaaccgca
    22201 caatgccccg cccccgaaag gcaacgtcct cacccgctgg tccttctgga aaaaagccgg
    22261 agattttctg gaaaaaaatt ataagcgact ggaaacgttc tacggccggg tgctgaagtt
    22321 ttcgctccgg cacagggcgg caatgctttt tgcaacgctg atggttttcc tgttcagcat
    22381 tttcattatg atattcagcc tcggcaagga atttctgccg cccgaagacc agggcaattt
    22441 tatcattcgc ctggaggcgc ccatcgatta ttccgtggaa caggtggaaa agtacttcgg
    22501 gccggctgaa aaaatggtcc gcgacattcc ggagattaaa tcggtcttct acgttcaggg
    22561 ctatcaggga tacgccaccc gcggcattat gatcgtcggg ttaattccca aggcggaccg
    22621 tgaaaaaagc caggaagata tcaaaaaaat tgtccgcatg aacctcaaac agattcccgg
    22681 actgaaggtg tcggcggaag atgtttcgct ggtcggcggc ggcatcagac aggtgccgat
    22741 tcagtacagc atccgcggac aggatttaaa cgaattgcag aagtacgccc ggcagatcac
    22801 cacggaattc gccaaacgac cgggaattgt ggacgtggat acctccctgg aagcggggaa
    22861 accggaattc aaggtttata tcgaccgcga caaggcggcg gatctgggtg tggatgtggc
    22921 cacgatcgct gaagccatca atttgctggt gggcggagaa ctggacatcg ccaggtataa
    22981 ggacgagaag aagggcaaac gctatgatat ccgggtaagg ctcaacgccg aggatcggga
    23041 aagttccgaa gccatgcaga agatttatgt gcgcgcccgc gacggaaagc tggtggaact
    23101 gagcaatgtg gtgagaatcg ccgaaggcac cggccccagc gtcattaacc gcgcggaccg
    23161 tcagcgggca atcaccattt ttgcctctct ggaaggaaaa ccactgggcg aagcccgaaa
    23221 cgagctggat gaaatcgccg ggcggattct gccggcggat tacgtgccga agtattacgg
    23281 catggccgag gttatgcagg aatctttcat cttcctgctc tttgccctga ttctgggtat
    23341 tatcatggcg tacatgattc tggcttcaca atttgaaagc ttcattcatc cggcaacggt
    23401 tttgctttcc atgccgctgt cattcatcgg agcgttcggc gctttgtttc tcacgggaaa
    23461 aacgctcaat atcttcagcc tcatcggtct gattcttctg atgggactgg tgaagaaaaa
    23521 tgccattttg ctggtggatt acaccaatgt tctgcgggaa cggggcatgt cgcgccgcga
    23581 cgccatcttg caggccggac cggtacgaat gcggccgatt ctcatgacca cctttgccat
    23641 gattttcggc atgctgccga tcgcgtttgc tgttggggaa ggcgcggaaa cccgcgctcc
    23701 gatgggcatc gcggttatcg g
//
LOCUS       DAOOLB010000029        23270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_2108_length_23270_cov_43.771441, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000029 DAOOLB010000000
VERSION     DAOOLB010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 23270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23270
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2108_length_23270_cov_43.771441"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..914
                     /locus_tag="PLF77_05390"
     CDS             195..914
                     /locus_tag="PLF77_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPC08324.1"
                     /translation="MKEQLLLLIKIQECDSELVKLSAKKKTLPEKIETLNKEFNAFED
                     GIKTSKVKYDELKLRHTDSENKIKKINESIVKTKERMLEVKNNKEYQAMLKEIEIAEK
                     SRGEIETSIISILDELDKLSVLVKKDDDVLKERRHQYEQEKKTIEDELNAVDTDAANW
                     EQKRGDLQRNVPEDLLVRYERIKKRNRGVGVISVWKSVCNGCHMNIPPQLYNEVQKSE
                     ELFSCPNCNRIIYFKDMVKPS"
     gene            920..1330
                     /locus_tag="PLF77_05395"
     CDS             920..1330
                     /locus_tag="PLF77_05395"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HPC08325.1"
                     /translation="MSEKYYKLFSDGACRGNPGIGGAGAVITDDADHVIWEGKEYLGH
                     CTNNIAEYKALILGLKGALSQGYRNLQIFLDSELLAKQINGAYRVKNENLQTLMKEVR
                     HLLVSFDRVEVKHVARLYNSHADKLANLAIDEKN"
     gene            complement(1357..1836)
                     /locus_tag="PLF77_05400"
     CDS             complement(1357..1836)
                     /locus_tag="PLF77_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08326.1"
                     /translation="MLKDYQMQLLGFLEQLEMEISNLYKSFAEQFPGHQDLWAEMSRE
                     ELQHAEYVRTLKAFALSGHVTFDEKMTKTYTIRSIIDDIRDKYKKTENHQYSVVNALS
                     FSLSLEQSIIEHKFYNYFSSNDPGAVLLINNIREETSAHESQVKKALDEEKKRITVR"
     gene            complement(1856..3001)
                     /gene="proB"
                     /locus_tag="PLF77_05405"
     CDS             complement(1856..3001)
                     /gene="proB"
                     /locus_tag="PLF77_05405"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HPC08327.1"
                     /translation="MENIRQNIITRAKKILIKVGSAVLTGDDGLDLKIIDSLVLEMAN
                     LAKKGYAVVLVTSGAIVSGKHRLKITENLKSIPEKQAAAAIGQGRLMRVYSKAFEKNG
                     IYVAQILLTLSDLTDRQRYLNIRNTLSTLMAWGTIPIINENDTVAVDEIKFGDNDNLA
                     AMIANIIEADLFINLTSIDGLYTCNPASSKNSKLISVVSEFSEEIEAAATEETSSSGT
                     GGMKSKVQAAKKVTAIGIPCIIAPGKKKNILTDIMTGREIGTLFLPMTDRLNSKKYWI
                     AFTLRPRGKLVIDDGAKKALLEEGKSLLPSGIIDIEGRFDHGDPVICVDNNGAILAKG
                     LVNYNSIEINKIKGLKTKQIGQVLGHKDYDEIIHRDNMAVTGQHYKK"
     gene            complement(3021..4016)
                     /gene="obgE"
                     /locus_tag="PLF77_05410"
     CDS             complement(3021..4016)
                     /gene="obgE"
                     /locus_tag="PLF77_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPC08328.1"
                     /translation="MKFVDEAKIYVKAGHGGSGCVSFRREKFVPKGGPDGGDGGKGGD
                     VIFRTTGSHHTLLDLKYKQHQIAKNGGHGSGNHRTGKSAPDLEISVPVGTIIKNFDTG
                     EELADLSKAGQTYIAARGGIGGKGNSHFATSTHQTPRFAQEGMEGDEFTLKLELKLLA
                     DVGIIGFPNAGKSTFISRVSAAKPKIADYPFTTLTPHLGVVQYADDKNFVIADIPGLI
                     PGAHEGMGMGDKFLKHVERTSLLLHIIDVSMTPHTDAWSNFSTINDELKKYNPALAEK
                     EQIAAINKIDLPFVREKIKKEVALFKKKGIILHAFSAVTGEGVKEILNRIAEKLK"
     gene            complement(4212..4466)
                     /gene="rpmA"
                     /locus_tag="PLF77_05415"
     CDS             complement(4212..4466)
                     /gene="rpmA"
                     /locus_tag="PLF77_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002778821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPC08329.1"
                     /translation="MAHKKGQGSSRNGRDSNSQRRGIKVFGGEQIHAGSIIVRQVGTK
                     IHPGNNVGMGKDFTLFSLINGVVKYERLDKTRKKVSVYAA"
     gene            complement(4479..4790)
                     /gene="rplU"
                     /locus_tag="PLF77_05420"
     CDS             complement(4479..4790)
                     /gene="rplU"
                     /locus_tag="PLF77_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPC08330.1"
                     /translation="MYAVIKTGAKQHKVSEGDILSVEKLDGNKGDEVVFSDVLMVSDD
                     KEIKVGKPFVDGAKVVGEIIAQKKGPKLTVYHMKRRKGFHKKTGHRQNLTSMKIKKIS
                     I"
     gene            5129..5956
                     /locus_tag="PLF77_05425"
     CDS             5129..5956
                     /locus_tag="PLF77_05425"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC08331.1"
                     /translation="MNAGAPVALISGCSTGIGRFLTTELAKRGWQVFATARNHAAIED
                     MKAPNVQTAALDVTDEKSIKTCVESVMANAGKIDMLINNAGLLLIGPLSELEISELRR
                     EFETNVIGLAALTHAVVPHMIKRTSGKIVNISSISGVLNTPFSGAYCSTKAAVTSYSD
                     VLRMELKPFGIDVITVQPGGIRSNLSDNADKGLERFRKTPYAPITDFIVDRAHMSQQG
                     ATPTDVFARQLVDKLVKSKTPRIIRIGKGATMYPFVAKLPRTFTDRLLSRKFGLDKL"
     gene            6094..6963
                     /locus_tag="PLF77_05430"
     CDS             6094..6963
                     /locus_tag="PLF77_05430"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HPC08332.1"
                     /translation="MKYGIIANPVSGKRSVDDKIKILKEVAGILKDATIAGLDTKSPE
                     EFSTAAHDLSQKVARLLVAGGDGTFSLVMNSVSRDIPLGFLPFGSGDVLRHTFNLPRC
                     PKENALLIQKGKEYLLDVICCDGKTKALFASIGIEGSILLERDNYLENNLTGFTAYGL
                     STLKILFDGYIKADLRVLMDKKEIRTFNSLTAVITKLPYYGYGFKVVPHAQFDDGYLH
                     LLILNAGPIGTLYALATSLAGGNRMGEYYKAKEIDITTSTEQLLQRNGDPEKEKRNNF
                     SFRILPKELKLIF"
     gene            7077..7763
                     /locus_tag="PLF77_05435"
     CDS             7077..7763
                     /locus_tag="PLF77_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPC08333.1"
                     /translation="MRQFNSDRRLIVKTAQKLVEKGYLAATGGNISVRIKKGNAFAIT
                     PSNRNYMTMTAADICILDEHLNVLSGPWKPSMEAALHGAVYRTRGDVNAVIHTHQVYA
                     GALTIIKTPLPALFDEQVRFLGKTVAVIPYAPSGSVPLKNNVLRHIKNGNNAFLIQNH
                     GALVLGHDMDRAVHNVEILERYSLAYLLAICSGYRVSKIPMRMRETFFKMLRQEQKKL
                     QKDHCGSRKK"
     gene            complement(7782..8483)
                     /locus_tag="PLF77_05440"
     CDS             complement(7782..8483)
                     /locus_tag="PLF77_05440"
                     /inference="COORDINATES: protein motif:HMM:NF015004.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HPC08334.1"
                     /translation="MRIWDVNPGYLNRQSLLGEHRELHAIVSIIQHNKKGYSRHPETL
                     RWHGFGWALSQRHRLLAAEMNLRGFTDRSPAMIKTQPQKWPHVFVDSPTSQFSILKKK
                     YVNKEQGRIPLPNSVQQLWAQHQYSVMARDEAEYKYIGGWVTSRKSGKGIHEIYPELV
                     SLLRCPPVEANLRNALEQMWNFVCAYAPHPATAMKKESSRHLLKQIQRTAVLHDIVYL
                     KESTALGELPAWQIG"
     gene            complement(8505..10982)
                     /locus_tag="PLF77_05445"
     CDS             complement(8505..10982)
                     /locus_tag="PLF77_05445"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HPC08335.1"
                     /translation="MRHGKIDELLSSAEKPSRYIGTEVNAVRKDKADVRFLLAFPDTY
                     EVGMSHLGLQILYSIINDIPYASAERCFAPWPDREKILRDKKLPLTSLERQIPLQNFD
                     MIGFSLQYELSYTNMLNMLDLGGIPIDRKERRDGHPLIIAGGPCCFNPAPLADFIDAF
                     VIGEGEEIVGDIVAAVREGKIKSISRNGLLGELANIPGMYVPAVHHDNEIIKKRTVSD
                     LNAWRHPLKPVVPLMQTIHDRITLEIARGCTRGCRFCQAGMIWRPYRERNAALLMEMA
                     EKMLAATGHEEISLLSLSSGDYSCLEPLVKGLMNRYCQKRVALALPSLRVESLNTTLM
                     EEIRRTRKTSFTLAPEAGTDKMRLVINKGNTSEDLLSTADKIFAAGWKSIKLYFMLGL
                     PHETQEDWEGIVNLSHETLRAAKNRGQVTISLSTFVPKPHTPFQWEKQISLAETYERQ
                     DFIRTRIKNRNLSVKWHDARMSFLEGIFSRGNEQAGKLLGNAFRKGCRFDGWSDILRF
                     NLWEEAIAESDIHPDDYLRERGTDEKLPWGNIDCGVSREFLLEEKQKAFQQTATADCR
                     FETCRNCGACDFSITKNILAADNENKSVLPSSLAPETSSAQEEKYRVMFNKMESAGFL
                     SHLELATALVRALRRSSINLAYTGGFHPHPKISFATATAVGMESRQEYMDITAQEYSC
                     SLDLLRTEINTLLPTGIKILEIRKLSPEENAIAQATRGFEYELQLPAATDAPRMSAIK
                     ANIDQFLAASNFNIKKISKGKTITKDIRPFVQMLSLDRDKKIIKFIVSFVQEGSARPV
                     DIIAHILKSDPDESRQIRVVKTKTLLG"
     gene            complement(11018..11416)
                     /locus_tag="PLF77_05450"
     CDS             complement(11018..11416)
                     /locus_tag="PLF77_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08336.1"
                     /translation="MKLIEEIKLDNGLDLRIFDLSRSIATDTVKVEVSFQTNVLLKES
                     FFTSTEDYRLVKNIMGDELAYEHTMERTFVSKDNEDSTRNELISTFKHNSLGYLSAAN
                     FAQKMALSKLREIKSNPHKYRSHAQSDKKA"
     gene            11529..12386
                     /gene="purU"
                     /locus_tag="PLF77_05455"
     CDS             11529..12386
                     /gene="purU"
                     /locus_tag="PLF77_05455"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF004684.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HPC08337.1"
                     /translation="MNNVNAVLLLSCADSKGLVARISDFIFRFDGNIVHADQYTAKAE
                     KLFFMRVEWELNGFALDRDDIPVAFAPLAKQYQMKWDLHFTDYVPRAAIFVSRHLHCL
                     HDLILRRDMGELAADIKAVISNHEDARDLAEQYGMQYFYFPVTGKNKKQQEKKELRLL
                     RDLHIDLVVLARYMQILSGNFLKYYPSRIINIHHSFLPAFAGRNPYQQAHDRGVKIIG
                     ATGHYVTAQLDEGPIIAQDVVKISHRDTVTDIQRKSKDLERIVLARALRLHLENRILV
                     YGSKTIVFE"
     gene            12557..13375
                     /locus_tag="PLF77_05460"
     CDS             12557..13375
                     /locus_tag="PLF77_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08338.1"
                     /translation="MSNSNIFVLCQPDKGKSCGACCGLYNYVDSSRSSLARRLRSRTK
                     RFRETVKSPADLKRYATETLVAEDFKKRYDVIHCCEYLGFLDHDEKKVGCLVHPEQNA
                     GCDMRDVSFYGRELCAGHLCPSHYFIPQSQSQILIKIIDDWYLYGLVLTDIDLVVQYF
                     RLIADRVGAELKPDVFDHDELKEIALDYFGWKLNWPFRSQEANRLGKYYFDGSQYMIS
                     HIEYEKFGREVSVLNSILLSLCSSFKSAVQLEEAEKMIWANIEKFVSTYKRHVS"
     gene            13565..14098
                     /gene="hpt"
                     /locus_tag="PLF77_05465"
     CDS             13565..14098
                     /gene="hpt"
                     /locus_tag="PLF77_05465"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPC08339.1"
                     /translation="MDQASRKILFPKETIQKRVREMANQISKDYAGKELILIGVLKGA
                     FIFMADLIREINIPCRVDFARLASYGAGSESSGKVVLTKDIETSIKDKDILIVEDILD
                     TGLTMQYFVEWLKERNPKSLKTCVFLDKRKRRKVPLEADYVGFTIDDGFVVGYGLDFN
                     EMYRFSPDIYTIHSNKL"
     gene            14180..15214
                     /locus_tag="PLF77_05470"
     CDS             14180..15214
                     /locus_tag="PLF77_05470"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HPC08340.1"
                     /translation="MIIQCRQCRTKFRFDDSHMQGSGIWLRCSRCGHVYFQENPFAKR
                     TDSGALPLTEPDLETKPVNEEEKTAPSETVSVPPEEAPSIIRRDEHVLKSIDKIMTAR
                     KRDREGIDLDIATFRDEKVGEADGQTGDIVQEEQEVNVEETDHQKKTLPKGSGRIWKT
                     ALWSVLVIGVIPLLIYFFILPQYGAQLVGSGEYLFKKISELRGAPELPTSGAYINQMV
                     KLQNVRYRRINNYILGQIGVLEGTALNRADFPLSRVQVKGEIVDAYSVVLGERSSYAG
                     NILTDEELINLSEEEILMKLLRPEGLNNANDRIMPNGQIPFMIVFPSEPSGVIKTTVM
                     TIGAERLLEP"
     gene            15285..16163
                     /locus_tag="PLF77_05475"
     CDS             15285..16163
                     /locus_tag="PLF77_05475"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08341.1"
                     /translation="MRYEGNIFRPFSEANSYLLQCTVGCSHNQCTFCGMYKDKKYRER
                     SLDEIMEDIAMAKDHYGDLEKVFLCDGDAIALKTDMLLTILKKLYGTFPSLRHVGSYV
                     GPQSTLSKSLEELQTLRAAGLTKAYIGVETGDDELLKKIKKGVGYEEMLQAGRNLIAA
                     GINLSAMVLLGLAGRGKASRRHALATAKICNEMKPQYLAALTVTPVPGTVLYRQVQRG
                     EFELLDAFETLEEMKQIFENITADNMKFVGTHASNYLPVVGTLQKDKQKMIAMVDEVL
                     KNRDERMLRPDHMRGL"
     gene            16340..17677
                     /locus_tag="PLF77_05480"
     CDS             16340..17677
                     /locus_tag="PLF77_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08342.1"
                     /translation="MMANVMDKYFNMRIILCVALMSMVLLLQACSINQVYLSDSDEED
                     YSAEDITDQEMMRVAKVKLQSTGMVKRAFDIEERHRKLTLRETRQLLKILSQRFKKSG
                     LSVGEFEDLVLERIGRDLEIISAFNERLKNAEAKEKDHGYELCKKNELLEVIKGSEDI
                     VEEYIEDMVNGILPEEFETSLSEITRLERTKEDDIVPGSFKPTCFKGALITMLVALRG
                     DRANVSTLKLSGGTWSNSSIIFHTIPMPGKISKSSIHPDFESFTYYYKGASSSSQGEL
                     GFPHSGYFAVGYGARKSYKGIANPEDCSSWIFHQVLQTQYPVTSYDVMQFFRIKTGTG
                     IRHTGYAATSEYGWLNSVFTPVEKITKKNARAMIKPGYVLCIRRPDEDGKVIRNQNYG
                     VSGHMAIALFATDTHIYAVACTRDVPLMEGIGIQKISFWKERNSDIMILRMNK"
     gene            complement(17824..18462)
                     /locus_tag="PLF77_05485"
     CDS             complement(17824..18462)
                     /locus_tag="PLF77_05485"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPC08343.1"
                     /translation="MPEILKPGTEISEDAMINRAVTILTDGGIIAYPTETFYGLGVDA
                     THEEAIGKIFAVKGRNFKNPVSLIIAKPEDINMLVTDVPETAKKLIDAFWPGALTIVF
                     QASDTVSPLLTAGTGKIGLRVSSHPIALKIVQNMKKPLTATSANLSGAPECSTASEVA
                     GQIGGKIDAILDGGKTPGGASSTLIDVTLNPPVVLREGSISRQSIKEHLHVI"
     gene            complement(18447..18956)
                     /gene="purE"
                     /locus_tag="PLF77_05490"
     CDS             complement(18447..18956)
                     /gene="purE"
                     /locus_tag="PLF77_05490"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPC08344.1"
                     /translation="MVKRNALISVVMGSDSDLPVMTEAAKVLEEFGIAHELILTSAHR
                     TPLRTTQFASAAAGRGIKVIIVGAGAAAHLAGVIASQTPLPVIGVPIDATALNGLDAL
                     LSTVQMPGGIPVATMAIGKAGAKNAALFAVRMLALDDKTLEKKLRDYIRKIAKDVEKK
                     QENLTCRKS"
     gene            19152..19880
                     /locus_tag="PLF77_05495"
     CDS             19152..19880
                     /locus_tag="PLF77_05495"
                     /inference="COORDINATES: protein motif:HMM:NF019443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HPC08345.1"
                     /translation="MKNNLYEFLAGNDYPGRGICIGMTPGGKAVIAYFIMGRSTNSRN
                     RVFDPIEGGIRTKAADPSKMTDPHLIIYNPVLTFDKTTIVTNGDQTNTIYDFMSQNKF
                     PGFPFEAALHTRTFEDDGPNWTPRISGMVDMRTGAYKINILKSCDGDEKSVQRFTFDY
                     VQPLPGEGHFISTYKCNGTPIPSFQGEPMRVTIDEEDPDRYAAKLWKALNEENKVSLF
                     VRAIDLTTQTCKDVIINKYKAVEE"
     gene            19883..21061
                     /locus_tag="PLF77_05500"
     CDS             19883..21061
                     /locus_tag="PLF77_05500"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF005492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HPC08346.1"
                     /translation="MNEIQLKYGCNPNQKPARIFMADGSHLPVEVLNGKPGYINFLDA
                     FNSWQLVKELKENTGMVAAASFKHVSPAGAALGYPLDRVLRKMYHIDSKIKLSPLACA
                     YARARGADRMSSFGDWIALSDVCDVSTAKIIKNEVTDGIIAPGYEPEALEILKSKKSG
                     NYNVVKINPDYVPVSLEHKQVYGITFEQGRNDLKIDSRMLENIVTENKTLTDAQKRDL
                     VLALITLKYTQSNSVCYVRDGQVIGVGAGQQSRIHCTRLAGQKADLWQLRHMPKVLNL
                     PFRDDVAKPNRDNAIDVYLGETPEDVTGDDVWGQTFTKQPKPLTKAQKQKWLSKVTGV
                     CLGSDAFFPFGDNIERARRSGVTAIVEPGGSIRDQQVIDTCNKYGIVMAFCGLRLFHH
                     "
     gene            complement(21208..21957)
                     /locus_tag="PLF77_05505"
     CDS             complement(21208..21957)
                     /locus_tag="PLF77_05505"
                     /inference="COORDINATES: protein motif:HMM:NF017062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF707 domain-containing protein"
                     /protein_id="HPC08347.1"
                     /translation="MMKNKNLVIAAVGDSSLHKQWKSGNPAFDLILIYYGNSDLIFFD
                     YSKDVMICIKQNGQKFPLIKSFIETNLELISQYDYVWLPDDDISISADDLNRLFETAR
                     TYSLQICQPALISLHGDVTHAITKPRVGNTLRFTNFVEVMMPLFDVKTLLSLRNDFDL
                     SQSGWGLDATWSHRLNSPKDKIAIIDDITAIHTKPVGRDYSRFRIKPETELKHLLEKY
                     NIAFVMKNYSSLLNNGSHKIPWYRNFFRLSS"
     gene            complement(22262..>23270)
                     /gene="mutY"
                     /locus_tag="PLF77_05510"
     CDS             complement(22262..>23270)
                     /gene="mutY"
                     /locus_tag="PLF77_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HPC08348.1"
                     /translation="DPYRIWISEIMLQQTQVDTVLPYYHRFLKTFPSVSSLASAPLQD
                     VLKAWENLGYYSRARNLHAAAKVIVEIFGGRIPDNLEEIKTLPGIGQYSAGAILSIAY
                     KQALPAVDGNVRRILCRVFAIRKPLDDAQEQKQLQKLAASLIPVNHPGDFNQALMDLG
                     ATICKSKNPDCSRCPIGNLCRARLRNLQNILPLTKKASKIPHRQAAAAVIRNAKGMLL
                     VVQRSASGLLASLWKLPGGFVEKGENMEKSLKHNVKEELGISIQPGKHLLSVNHTYSH
                     FRLTLHAYECHSLKGTPKPLGCQNWRWTSPADLKKLPLSKIDRLILEEILQCHLYLFR
                     K"
BASE COUNT         6142 a         5278 c         5712 g         6138 t
ORIGIN      
        1 agctcgaagc gcaagcggta gtgaggcaag ctgaacgata gtgaagcgta aaccgaacta
       61 tccaatcccg gtgtatcggg gttggatact attcctgcag cgaagcggaa tggattcttg
      121 ttaagagcaa gacgaaataa aaaaaatgac cctgaacttc atgtcaggga ttgaaaaaaa
      181 gaaggagaag atgattgaaa gagcaattat tattactgat aaaaattcag gaatgtgatt
      241 ccgaactggt gaagttgtcc gcaaagaaaa aaacactgcc cgagaagatc gagacactca
      301 ataaagaatt taatgcattt gaagatggca taaaaaccag caaagttaag tatgatgaac
      361 tcaagttgcg tcatacggat agcgaaaata aaatcaagaa aatcaacgaa agcatagtca
      421 aaaccaaaga aagaatgctg gaagttaaaa ataataagga atatcaggcg atgctcaaag
      481 aaattgagat tgcagaaaaa tcgcgtggcg aaattgaaac ctcgatcatt tccatactgg
      541 atgaattgga taaactttct gtgctggtca aaaaagatga cgacgtattg aaagagagaa
      601 gacatcaata cgaacaggaa aagaaaacga tcgaagatga attgaatgcc gtggacaccg
      661 atgcggcgaa ctgggaacaa aaaagagggg atttacagag aaatgttcct gaagatctgc
      721 tggtccgata cgaaagaatc aaaaaacgaa acagaggcgt tggcgtgata tcggtctgga
      781 aatcggtctg caacgggtgt cacatgaaca tcccccctca actctataac gaagtccaga
      841 agtcggagga gttgttcagt tgcccgaact gcaatcggat tatctatttt aaagatatgg
      901 tcaagccatc ataagggata tgtctgaaaa atattacaaa ctgttcagcg acggcgcctg
      961 ccgggggaat ccgggcatcg ggggcgcggg ggctgtaatc accgatgatg ctgatcatgt
     1021 gatttgggaa ggaaaggaat atctcggtca ctgcaccaat aatattgccg aatacaaagc
     1081 gcttattctg ggactcaaag gtgccctgtc tcagggatat agaaaccttc aaatattttt
     1141 ggattcggag cttctggcca aacaaatcaa cggcgcctac cgtgtcaaga atgaaaatct
     1201 gcagacactc atgaaagaag tccgtcatct tctggtgtct tttgatcgcg ttgaggtgaa
     1261 acatgtcgcg cgtctgtaca acagccatgc agacaaactg gccaacctgg ccattgatga
     1321 aaaaaattag tttttaggga ttgaaacgaa agatcattac cgaacagtta tccgtttttt
     1381 ttcttcatcc agggcttttt ttacctgcga ttcatgcgcc gaggtttctt ctctgatgtt
     1441 attgatcagc aggaccgcac cgggatcatt tgaactgaaa taattataaa atttgtgttc
     1501 gatgatggat tgctccaggc tgaggctgaa cgataaagcg ttgacgacgg agtattgatg
     1561 gttctctgtc tttttatatt tatcgcggat atcatcgata atcgatcgaa tcgtgtaggt
     1621 tttggtcatt ttttcatcaa aggtcacatg gccgctcaac gcgaatgctt ttaaagtgcg
     1681 gacgtattcg gcgtgttgca attcttcccg gctcatctca gcccagagat cctgatgtcc
     1741 gggaaattgc tcggcgaacg atttatagag attggagatt tccatttcca attgctcgag
     1801 aaaacccagc aactgcattt gataatcttt cagcatgtga cgttactttt ccgcgttatt
     1861 ttttgtaatg ttgtccggtg acggccatat tgtcgcgatg aatgatttca tcataatcct
     1921 tgtgtccgag aacctgaccg atttgttttg tttttaatcc tttgatttta ttaatttcta
     1981 tggaattata attaaccagt cctttcgcca ggatcgcacc attgttgtcc acgcagatga
     2041 ccgggtctcc gtggtcaaag cgcccctcaa tgtcaatgat tcctgaagga agaaggctct
     2101 tgccctcttc cagcagtgct tttttcgccc cgtcgtcgat cacgagtttg ccgcggggac
     2161 gcagggtgaa agcgatccag tacttcttgc tgttcaaacg atccgtcatg ggtaaaaata
     2221 aggttccgat ttcgcggccc gtcataatat ccgtcaggat atttttcttt tttccgggcg
     2281 cgatgatgca gggaatgccg atagccgtca ctttcttggc ggcttgaacc ttgcttttca
     2341 ttcctcccgt tcccgaggag gatgtttctt cagtcgccgc ggcttcaatt tcttcagaaa
     2401 attcagagac gacactgatc aatttcgaat tcttggatga ggccggattg caggtgtaga
     2461 ggccgtcaat gctggtcagg ttaataaaga gatcggcttc gataatatta gcaatcatcg
     2521 cggcgagatt gtcattgtcg ccgaatttta tttcatccac cgcaaccgtg tcattttcat
     2581 tgataatggg aatcgttccc cacgccatca gtgtggaaag ggtgttgcgg atattcaggt
     2641 atcgctggcg atcggtcaaa tcggaaagcg tcaaaagaat ctgcgccaca taaataccgt
     2701 ttttctcgaa ggctttggaa tagacgcgca tcaatctgcc ctgaccgatg gctgcggcag
     2761 cctgtttttc aggaatactt ttcagatttt ccgtgatctt caaccggtgt tttcccgaga
     2821 caatcgctcc ggaggttacc agcacgaccg cataaccttt cttggcgaga ttcgccattt
     2881 ccagcaccag agaatcgatg attttcagat cgagaccgtc atctcccgtc agtacggccg
     2941 aaccgacttt gatcagaatt ttttttgcgc gcgtgatgat attttgccga atattttcca
     3001 tgacatcaac tctaaccggt ttattttaac ttttcagcaa tcctgtttaa tatttccttt
     3061 accccttcgc cggtgactgc tgaaaaagcg tgtaatatta tgcccttttt tttgaataga
     3121 gcaacttctt ttttgatttt ttcccggaca aacggaagat caattttgtt gatcgctgcg
     3181 atttgctctt tttcggccag agccgggtta tattttttca gctcgtcgtt aatcgttgag
     3241 aaattggacc aggcgtcggt atggggggtc atggagacat cgataatatg caacagcaat
     3301 gacgttcttt cgacgtgttt cagaaattta tcgcccatgc ccatgccctc atgagcgccc
     3361 ggaatcagtc cgggaatgtc ggcaatgaca aaatttttat cgtctgcata ttgcacgacg
     3421 cccagatgcg gcgtaagcgt ggtgaagggg taatcggcaa tcttgggttt ggctgcggaa
     3481 acacgggaga taaaggtcga tttgcccgca ttggggaaac cgataatgcc cacatcggcc
     3541 agaagtttca gttcgagttt gagcgtgaat tcatcgcctt ccattccttc ctgggcgaag
     3601 cggggcgtct gatgcgttga ggtggcaaag tgagaattgc ccttgccgcc gatgccgcca
     3661 cgtgcggcaa tatacgtctg tcccgctttt gacagatcgg caagctcttc tccggtatca
     3721 aaatttttga tgattgttcc caccggaacg gagatttcca gatccggagc acttttcccg
     3781 gtgcggtgat tgccggaacc gtggccgccg ttcttggcga tttgatgctg cttgtatttt
     3841 aagtccaaca gcgtgtgatg acttcccgtt gtgcgaaaga tgacatcgcc gcctttgccg
     3901 ccgtctcctc cgtccggacc acctttcggg acaaattttt ccctccggaa actcacgcaa
     3961 ccgcttccgc cgtgtccggc cttgacataa atttttgctt catcgacgaa tttcataacc
     4021 ttgttcagcc gtatgttctt ttgagttaag ataaactata tttaatacgt tgatccctta
     4081 ttccgcatac gcgggataat ccggctagcc aattccaatg cacttcgttt ttggaattgg
     4141 agcctcatta aaaacaaaag gcgcttgaag taagcgcctt aaatggttgt aatcgatccg
     4201 atcgaatatt cttatgctgc gtaaacgctt actttcttcc tcgtcttatc aagtctttca
     4261 tattttacaa cgccgttaat cagggaaaaa agagtgaaat ctttgcccat tcccacgttg
     4321 ttgcccgggt gaatttttgt tcctacctgt cgcacaatga tcgagccggc gtgaatttgt
     4381 tctccgccga ataccttaat tccgcgtctc tgggaattgc tgtctctgcc attgcgggaa
     4441 cttccctgac cttttttatg tgccatgact tcctccgctt atatcgaaat tttcttaatt
     4501 ttcatgctgg ttagattttg acgatgaccg gtttttttat ggaaaccttt ccgtcttttc
     4561 atatggtaaa cagtaagttt cggtcctttc ttctgggcga taatttcacc gacaactttg
     4621 gcgccgtcca caaaaggttt gccgactttt atctctttat cgtcggagac catcagcaca
     4681 tcgctgaaaa cgacttcatc gcctttattc ccgtccagtt tctcgacgga taaaatgtcg
     4741 ccttcgctta ccttgtgctg ttttgctcct gttttaatca ctgcgtacat aacgtatcct
     4801 tatgaaaatt gaattaagac tgatctttat agacaaaacg tccccgtttg tcaataaatt
     4861 ttaatccgga caggagaaat atttctattt ctgcgaaaaa tggataagtt gctgatatca
     4921 tatgaaaagt aaaaaaatag aaacatttta tgccgtgata cggtgcactg gcaagaaaca
     4981 atttcaccat gtcggggcaa aaacgaattt gaaaaatact gtttattttt ctgcgcagtt
     5041 atgaaataag aaaaaaatac gaacacaacc atatactata agaaccatgt gccgttaaaa
     5101 tcatacagtt caaccaagga ggtttatcat gaatgctggc gcaccagtgg cgctcatcag
     5161 cggatgttca acgggaatcg gacgtttcct gaccacggaa ctggcgaaac gcggctggca
     5221 ggtgtttgcc actgcccgca accatgcggc cattgaggat atgaaagcgc caaatgtgca
     5281 aaccgccgcc ctcgacgtaa cggatgaaaa atcgattaaa acctgcgtgg aatcggtcat
     5341 ggccaacgcc ggtaaaattg acatgctgat taataacgcc ggtcttctgc tcatcggccc
     5401 cttgtcggaa ctggaaatca gcgagttgcg cagggagttt gaaacaaacg tcatcggact
     5461 ggcggctttg acccatgcgg ttgtgccgca catgatcaaa agaacatcgg gaaaaattgt
     5521 caatatcagc agcatctccg gtgttctgaa tacgcctttt tcgggcgcgt actgttccac
     5581 caaagcggcg gtgacctcct attccgatgt gttgcgcatg gaactgaaac ctttcgggat
     5641 cgacgtgatt accgttcaac cgggcggcat caggtcaaat ttatcggata acgccgacaa
     5701 agggcttgaa cgttttcgaa aaacgcccta tgccccgata accgatttta ttgtcgatcg
     5761 tgcgcacatg tcgcaacagg gtgcgacacc cacggacgtc ttcgcgagac aacttgtcga
     5821 caaactcgtg aaaagtaaaa caccgagaat catccgcatc ggaaaggggg cgacgatgta
     5881 tccctttgtc gccaaattgc cccgtacgtt tacggacaga ctgctttccc ggaagttcgg
     5941 gctggacaaa ctttaggaaa ttcgtcaatt aagaaaatag cgtttgttgg tgcgcgatta
     6001 ttttcttgac ataaaaaatc acggcactta tatttactcc atcaaaactt gctcctcgtt
     6061 cgaaaataca atgtggcagt gagatgattt gacatgaaat acggcattat cgcgaaccct
     6121 gtttccggca aacgctctgt agatgacaaa atcaaaattt taaaagaagt agccggtatt
     6181 ttaaaagacg ccaccattgc cggtctcgat acaaaatctc ccgaagaatt cagcactgca
     6241 gcgcatgacc tttcccaaaa agtggccagg ctgcttgtcg ccggtgggga cggaaccttt
     6301 tcactggtga tgaacagcgt ttccagagac atccctctgg gatttctgcc gttcggttcc
     6361 ggcgatgtcc tgcgccatac cttcaacctt ccccgttgtc ccaaagaaaa tgctctgttg
     6421 attcagaaag gcaaagaata tttgctggat gtcatttgct gcgacgggaa aacaaaagcc
     6481 ttgttcgcga gcatcggcat tgaaggaagt attctgttag aacgcgataa ctatctggaa
     6541 aataatttga ccggttttac cgcctatggt ctgtcgacat taaaaatttt atttgacgga
     6601 tacatcaagg ctgatttgcg tgttttgatg gacaaaaagg aaataagaac cttcaactct
     6661 ctgaccgccg ttattacaaa acttccctac tacggctacg gattcaaggt tgtccctcat
     6721 gctcaatttg atgacggata tttgcatttg ctgatcctga atgccggacc catcggcacg
     6781 ctctatgctt tggcaacttc tcttgcgggg gggaacagaa tgggagagta ttacaaagcg
     6841 aaagaaattg atatcaccac ttcaacggag caattgctgc agcgcaacgg cgatccggaa
     6901 aaagaaaagc gaaacaattt ttccttcagg attttaccca aagaacttaa actgattttt
     6961 tagtgaactg attcccccgt tcagccaagc ctttttgacc tggccgcaaa taaaaataat
     7021 ccgtagagtc caaaaaactc ggctgatctt aatgctattt ggaatggaga aactccatga
     7081 ggcaatttaa ttcagacagg cgtctgatcg tgaaaacagc gcaaaagctt gttgaaaagg
     7141 gttatcttgc ggcaaccggc ggcaatattt ccgtgcggat taaaaaagga aatgcttttg
     7201 ccataacacc gtccaatcgc aattacatga ccatgaccgc ggccgatatt tgtattcttg
     7261 atgaacacct caacgtgctt tcggggccat ggaaaccatc catggaagcg gctttgcacg
     7321 gtgccgttta tagaacacgc ggcgatgtca acgccgttat ccacacacat caggtttatg
     7381 cgggcgcgct gacaattatc aaaacgccgc tgccggcgct gtttgatgag caggtgcgtt
     7441 ttctgggcaa aacggtggcg gtcattcctt atgctccttc gggatccgtg cctctgaaaa
     7501 acaatgttct ccgacacatc aaaaacggca acaatgcctt cctgattcag aatcacggcg
     7561 cgctggtttt aggtcacgac atggatcgcg ccgttcataa tgttgaaatt ctggaaagat
     7621 attctctggc gtatcttctg gcgatttgca gcgggtacag ggtgagcaaa attcccatga
     7681 ggatgcggga gacgtttttc aagatgctgc gtcaagagca aaagaaactg cagaaagacc
     7741 attgcggttc gaggaaaaaa taaaagatac ggatgattgt ttcatccaat ctgccacgcc
     7801 ggcaattcac ccagcgccgt tgattctttc aggtagacaa tatcatgcaa aacggccgtg
     7861 cgctgtatct gcttcaaaag atgtcgtgaa gattcttttt tcatggcggt tgcgggatgc
     7921 ggcgcgtacg cgcaaacgaa attccacatc tgttcaagag cgtttctgag attggcttcg
     7981 acgggcgggc agcgaagcag cgaaaccagt tccggataaa tctcatggat tcccttgccg
     8041 cttttccggg atgtcaccca accgccgatg tatttgtatt cagcttcatc gcgcgccatg
     8101 accgaatatt gatgctgcgc ccatagctgc tgaacactat tgggcaacgg aatgcgtccc
     8161 tgttctttgt tgacgtattt ctttttcagg atggagaatt gagacgtcgg agaatcgaca
     8221 aatacatgcg gccatttttg cggttgcgtt ttgatcatcg ccggcgacct gtccgtgaaa
     8281 ccgcgcagat tcatttccgc cgccagtaat ctgtggcgct ggctcaatgc ccagccgaaa
     8341 ccatgccagc gcagcgtttc cggatgccgc gaatatcctt ttttattgtg ctggatgatg
     8401 gaaacaatcg cgtgcagctc gcggtgttca cccaataagc tttgccggtt taaataaccg
     8461 ggattgacgt cccaaattct cacaagctct ccttattgat aaagctatcc gagcagtgtt
     8521 ttggttttga ccaccctgat ttgacgcgat tcgtcggggt cggatttcag gatgtgggcg
     8581 atgatatcga caggcctggc cgaaccttcc tgaacaaagg atacgataaa ttttattatt
     8641 ttcttgtcac ggtctaggga aagcatttgc acaaaaggac gaatgtcttt ggttatggtt
     8701 ttccctttgg atattttctt gatgttgaaa ttggatgccg ccagaaattg atcgatgttt
     8761 gcttttatgg cgctcattcg gggagcgtcg gtagcggcag gcagttgcag ttcatattcg
     8821 aatccccttg tcgcttgagc gatagcattt tcttccggag atagctttct gatttccaaa
     8881 attttaatac ctgtgggaag cagagtgttg atttcagttc ttaataaatc cagactgcat
     8941 gaatattcct gtgcggtgat gtccatatat tcctgtctgc tttccatgcc tacagcggtt
     9001 gccgtggcaa aggaaatctt gggatgcgga tgaaaaccac cggtataagc aaggttgatg
     9061 ctgctgcggc gcaaggcgcg aaccagagcg gtggctagtt ccagatgaga caggaatccg
     9121 gcgctttcca ttttattaaa cataacccga tatttttctt cttgtgccga agaagtctcg
     9181 ggtgccaaag acgatggcaa cacagatttg ttttcgttgt ctgcggcgag aatatttttc
     9241 gtgatcgaaa aatcgcatgc tccgcaattc cggcaagtct caaaccggca atctgctgtt
     9301 gccgtctgct gaaacgcttt ctgcttttcc tccaaaagga attcacggct cacgccgcag
     9361 tcaatgttgc cccatggcaa tttttcgtca gtcccgcgtt cgcgaaggta gtcgtcggga
     9421 tgaatgtccg actcggcgat ggcttcttcc cagaggttga aacgcagaat atcagaccaa
     9481 ccgtcgaagc ggcaaccttt gcgaaacgca tttcccagaa gttttcccgc ttgctcattg
     9541 ccgcgcgaaa aaatgccttc cagaaaactc atccgcgcgt cgtgccattt gacgctgaga
     9601 ttgcggtttt tgatccgcgt gcggatgaaa tcctgccttt cataagtttc tgccagtgaa
     9661 atctgttttt cccattgaaa gggagtatgc ggtttgggga cgaaggtgga aagactgatg
     9721 gtaacctgac cgcggttttt tgccgcgcgc agggtttcat ggctgagatt gacgatgcct
     9781 tcccagtcct cctgtgtttc atgaggcagg cccagcatga aatagagctt gatggatttc
     9841 cagccggcgg caaatatttt atccgcggtg gaaagtaaat cttcggaggt gttgcccttg
     9901 ttgataacca gacgcatttt gtccgttccc gcttcgggcg cgagggtgaa actggtcttg
     9961 cgcgtgcgcc tgatttcttc cattaaagtc gtgttgaggg attccacacg cagggaaggg
    10021 agggccaacg ccacccgctt ttgacaatac cggttcatca ggccctttac caaaggttct
    10081 agacaactgt agtcgccgga gctgagggat aaaagcgata tttcttcatg gccggttgcc
    10141 gcgagcatct tttcagccat ttccataagc agggcggcgt ttctttcgcg ataaggccgc
    10201 cagatcattc ccgcctggca gaaccggcag ccgcgggtgc aaccgcgcgc gatttccagc
    10261 gtgatgcggt cgtgaatggt ttgcatcagc ggaacaacgg gtttgagggg atgcctccag
    10321 gcattgagat cgctgacggt tcgttttttg atgatttcat tgtcatgatg aacggcggga
    10381 acatacatgc cgggtatgtt cgccagttcg ccaagcagcc cgtttcggga gatggatttt
    10441 attttgcctt cgcggacggc tgcaacaata tcgccgacga tttcttcgcc ttcgccgata
    10501 acgaacgcat cgataaaatc agcaagcggc gcgggattaa aacagcaggg accgcctgca
    10561 ataatcagcg gatgtccgtc ccggcgttct ttacggtcaa tcgggatacc tcccaggtcc
    10621 agcatattga gcatattggt ataggaaagt tcgtattgca gtgaaaaacc gatcatgtca
    10681 aaattctgaa gcgggatttg acgctccaaa gacgtcaacg gcaatttttt gtcacgcagt
    10741 attttttccc ggtccggcca gggcgcaaaa caacgttctg ctgacgcgta agggatgtca
    10801 ttgataatcg aatagagaat ctgcaatccg agatgcgaca tgccgacttc gtacgtgtcc
    10861 ggaaaggcca gcagaaagcg aacatcggct ttatctttac ggacggcgtt tacttccgtg
    10921 ccgatgtaac ggctgggttt ctcggcggaa gataaaagct catcgatttt gccgtggcgc
    10981 ataatgattt tttccttttt gggcaaaggt cccggatcta tgcttttttg tcggattgag
    11041 cgtgggatct gtatttatgc ggattgcttt ttatttccct gagcttggat aatgccattt
    11101 tttgagcaaa attcgcggcg gagagatatc ctaaagaatt atgcttaaat gtgctgatta
    11161 actcattgcg ggtcgaatct tcattgtctt tggacacgaa cgttctttcc atggtgtgtt
    11221 cgtaagccag ttcgtcgccc ataatatttt ttaccagacg ataatcttcc gtgctggtga
    11281 aaaaagattc tttcagtaaa acgtttgttt ggaaggaaac ttccaccttg accgtatctg
    11341 tcgctatgga acgggacaaa tcgaaaattc ttaaatccag cccgttatct aattttattt
    11401 cttcaatcag tttcattttg ctcatctcaa tccatttggt ttacaagacg gttattaaca
    11461 tttttatact tgacctgcaa ctttaatcct tttatacgta gctcgcagag gaaactggtg
    11521 aatgttttat gaataatgtc aatgctgttt tgcttttgtc ctgcgccgac agcaagggac
    11581 tagtggccag aatatccgat tttattttcc gtttcgacgg caatattgtg cacgccgatc
    11641 aatatacggc aaaggcggaa aagcttttct tcatgcgcgt tgaatgggag ctcaacgggt
    11701 ttgctttgga tcgtgacgac attcccgtag cgttcgcgcc gctggccaaa caatatcaga
    11761 tgaaatggga tttgcatttt accgattacg tcccgcgcgc ggcgattttt gtttccaggc
    11821 atctgcattg cctgcacgat ctgattcttc gtcgggacat gggagagctg gcggccgata
    11881 tcaaagcggt catcagtaac catgaggatg cccgcgatct ggcggaacag tacgggatgc
    11941 agtattttta ttttcccgta acgggaaaaa ataaaaagca gcaggaaaag aaagagctga
    12001 ggctgctgcg ggatctccat atcgatctcg tggtgctggc gcgctatatg cagattctca
    12061 gcggcaactt tctcaaatat tatcccagca ggatcattaa cattcaccac tcgtttctgc
    12121 cagcctttgc cggcaggaat ccttaccagc aggcccatga ccgcggtgtc aaaattatcg
    12181 gcgcaaccgg tcattatgtg acggcgcagc tggatgaggg gccgattatt gcgcaggatg
    12241 tcgtgaaaat cagccatcgt gacacggtga ctgatattca gagaaaaagc aaggatctgg
    12301 aaagaattgt cttggcgagg gctttacgcc tgcatctgga aaacagaata ctcgtttacg
    12361 gatcgaagac tatcgtgttc gagtaaggaa gtattgtaat gttgtttgaa gacgtcacac
    12421 cggtgcggac cggtgtccgg aaaatgctgc aaaaaagaga ctccggcctt cgccgaaatt
    12481 atgaaagaga gcaatcaaca agtattttac aggttcatca gatctgataa tcattataca
    12541 gaccggataa ttatttatgt cgaattccaa tattttcgtc ctttgtcagc cggataaggg
    12601 aaaatcctgc ggcgcgtgtt gcggacttta taattacgtg gacagctccc gttcttctct
    12661 tgcacggcgt ctgcgttcgc gcaccaaacg tttccgtgaa acagtaaaaa gtccagccga
    12721 tcttaagcgc tatgcgactg aaacgctggt cgcggaagat ttcaaaaaga gatatgacgt
    12781 gattcactgc tgcgaatatc tggggtttct cgatcacgat gaaaagaaag tcggctgtct
    12841 tgtgcatccg gaacaaaacg cagggtgcga tatgcgcgat gtttcgtttt acggtcgcga
    12901 actgtgcgcg gggcatctct gtcccagtca ttattttatt ccgcagtccc agtctcaaat
    12961 tctgatcaaa atcattgacg actggtatct gtatggtctg gtgctgacgg atattgatct
    13021 ggttgttcag tattttcgtc tcattgccga cagggtcggc gccgaactca aaccggacgt
    13081 tttcgatcat gacgagttaa aagaaattgc cctggattat tttggctgga aactgaactg
    13141 gccgtttcgg tcgcaggaag cgaatcgtct gggcaaatat tactttgacg gctcgcaata
    13201 catgatcagt catatcgagt acgaaaaatt cggcagagag gtttctgtgc tgaattcgat
    13261 tttattgagt ctatgttctt cttttaaaag tgcggtccaa ctggaggaag cggagaagat
    13321 gatctgggcc aatattgaaa aatttgtatc cacatataag cggcatgttt catgaggttt
    13381 gctgagtaca aataacagac ggtatgatgc gttgcccgaa tttttcacgc cgagaaatat
    13441 gcaaagccgc actccggaat ttcgtctgat gctcagagga taatttttat tgacataaca
    13501 caacgacttt ggataatctt aatacccttt tgggtaattt ataagcaatt taaggggatg
    13561 tgcaatggat caggcgtcca gaaagattct ttttcccaaa gaaaccattc aaaaacgggt
    13621 acgggaaatg gcgaatcaaa tatccaaaga ttatgcagga aaagaattga ttttgatcgg
    13681 cgtactgaag ggcgctttta ttttcatggc cgatttgatt cgggaaatca acattccctg
    13741 cagggtggat tttgcaaggc tggccagtta cggcgccgga tctgagagct ccggcaaagt
    13801 cgtcctcacg aaggatattg aaacatccat caaagacaag gatattttga ttgtcgagga
    13861 tattctggac accggactca ctatgcagta ttttgtggag tggctgaagg aaagaaatcc
    13921 taaatctctc aaaacatgtg tttttctgga taaacggaag aggagaaaag tgcctttgga
    13981 ggccgattat gtggggttta ccattgacga cggcttcgtg gtaggttatg gactcgattt
    14041 taatgaaatg taccgctttt ctcctgatat ttatactatt cattcgaata aactgtaaga
    14101 cataatatca gagaaacttt cagaaattca tcatgcaatt tttgaaagca ccgtgtcgca
    14161 agcagtaagg acagacgtta tgattattca atgccggcaa tgccggacaa agtttcgctt
    14221 cgatgattcg catatgcagg gaagcggtat ctggctcaga tgcagccgtt gcggccatgt
    14281 gtattttcag gaaaatcctt ttgcgaaaag aaccgattcc ggtgcgcttc cgctgaccga
    14341 accggacctt gaaacgaaac cggtcaacga ggaagaaaaa acagctcctt ccgaaacggt
    14401 gtcagtgccg ccagaagaag cgccttccat catccgcaga gacgagcacg ttctgaaatc
    14461 catcgataag atcatgacgg ccagaaagcg tgaccgcgaa gggatcgatc tggacatcgc
    14521 cactttccgg gatgaaaaag tcggcgaagc agacggacag acgggtgaca tcgtacaaga
    14581 ggaacaagaa gtcaatgttg aagagaccga ccatcagaag aaaacgctcc cgaaaggttc
    14641 cggcagaatc tggaaaacgg ctctctggtc ggttctggtc ataggggtta taccgctgtt
    14701 gatctatttc ttcattttac cgcaatatgg cgcacagctg gtcggcagcg gtgaatatct
    14761 cttcaaaaag atcagcgaat taagaggcgc tccggaactg ccgacatcag gggcttatat
    14821 caaccagatg gtgaagcttc agaatgtgcg ttaccgcaga atcaacaatt acattctagg
    14881 ccagatcggt gttctcgaag gcacggcgct gaatcgtgcg gatttcccgc tgtcccgggt
    14941 tcaggtgaaa ggtgaaatcg tggatgccta ctcggtcgtt ttgggtgaac gttccagtta
    15001 cgcggggaat atcctgaccg atgaagaact gattaatttg tctgaagaag aaattttaat
    15061 gaaactgctt cgtccggaag gtcttaataa cgccaacgac aggattatgc ccaacggaca
    15121 gatccctttc atgattgttt tccccagcga accctcgggt gtcatcaaga caacggtcat
    15181 gaccatcggc gcggaacgtc tccttgagcc ctgatcggga tattcattga ccgcagtatt
    15241 caaaaaatca gcacgcattc gtccccgatt gaaaggagtc aaaattgaga tacgaaggaa
    15301 atattttccg gccgttcagt gaagccaaca gttatcttct gcagtgcacg gtcggctgct
    15361 cgcataatca atgcaccttc tgcggtatgt acaaggacaa aaagtacagg gaacggtcgc
    15421 ttgacgaaat catggaagac attgccatgg ccaaagacca ttacggcgat ctggaaaaag
    15481 tttttctctg cgacggcgac gcgattgcgc tgaaaacaga catgctgctg accattctga
    15541 aaaagctgta cgggacattt ccgtcgctgc gccatgtcgg aagctatgtc ggaccgcaaa
    15601 gtacgctgtc caaatccctg gaagaattgc aaaccctccg ggctgccggt ttgaccaagg
    15661 catatatcgg tgtggaaaca ggcgatgacg aacttttgaa aaaaattaaa aaaggcgtcg
    15721 gttatgaaga aatgctccag gctggtcgga acctgatcgc ggcgggaatc aatctgtccg
    15781 cgatggtgct tctgggcctg gccgggcgag gaaaggcctc tcgacggcat gctctggcca
    15841 cggcaaaaat ctgcaatgaa atgaaaccgc agtatctggc cgcgcttacc gtgacgcctg
    15901 ttccgggcac ggtgctgtac cgtcaggtgc agcggggcga gtttgaattg ctggacgcct
    15961 ttgaaaccct ggaagaaatg aagcagatat tcgagaatat caccgccgac aatatgaaat
    16021 ttgtcggcac gcatgcctcc aattatcttc cggttgtggg gacgctgcag aaagacaaac
    16081 agaaaatgat cgccatggtc gatgaagttt tgaaaaacag agatgagcgg atgttgcgtc
    16141 cggatcacat gcgcggactg taaatgattt tttctgcaat gaagcagcag gtcgtttaga
    16201 cgaaaattgt gtttatcggg gacatggatg tatgatcatg gtatccggtg ttcaagattt
    16261 tcttgacacg aaagcagggt gtcttttatg cttcggggca atatcagggt aaaaaaatat
    16321 ttttaaatgg cggcggacaa tgatggcaaa tgtgatggat aaatatttca atatgagaat
    16381 catcttatgc gtcgcgctga tgagcatggt gttattgttg caagcctgtt caatcaatca
    16441 ggtttatttg tccgattcag acgaggagga ttacagcgcg gaagatataa cggatcagga
    16501 gatgatgcgc gttgcaaaag tgaaattaca gtcaacgggt atggtcaaga gagcctttga
    16561 tattgaagag cgccacagaa aactgaccct cagggaaacc agacaattgc tcaagatttt
    16621 gagccagcga tttaagaaaa gcggcttgtc cgtcggtgaa tttgaagatc tggtgctgga
    16681 gaggataggt cgcgaccttg aaatcattag cgcctttaat gagcggctga aaaacgcgga
    16741 ggcgaaagaa aaagatcacg ggtatgaatt gtgcaaaaaa aatgaattgc tggaagtgat
    16801 taaaggttct gaagacattg tggaagaata tattgaagac atggtcaacg gtatcctgcc
    16861 ggaggaattt gaaacgtcgc tgtctgaaat caccagactt gaaaggacca aagaggacga
    16921 cattgtgccc ggatcattca aacccacctg ctttaaagga gccctaatta ccatgctggt
    16981 cgctttgaga ggcgaccgtg ccaatgtgtc gacattgaaa ttatccggcg ggacgtggtc
    17041 aaactccagc atcatctttc ataccattcc gatgccgggc aaaatcagca aatcgtcaat
    17101 tcatccggat tttgaatcat ttacctatta ttataaaggc gcttccagtt cctcgcaggg
    17161 ggaactgggg ttcccgcaca gcggatattt tgccgtcggc tacggcgcca gaaaatccta
    17221 taagggcata gccaatcctg aagactgctc atcctggata tttcaccagg tattgcaaac
    17281 gcaatatccg gtgacctcgt atgacgtgat gcagtttttc agaattaaaa ccgggacggg
    17341 aatacgtcac acaggttacg ccgccacgtc tgaatacggc tggcttaact cggtgttcac
    17401 cccggtggag aagataacga aaaaaaatgc gcgggcgatg ataaaacccg gttatgtatt
    17461 gtgtatacgg cgaccggacg aagacggcaa agtcattcga aatcagaatt acggagtttc
    17521 cggtcatatg gcgattgcac tatttgcaac ggatactcac atttatgccg tggcctgcac
    17581 acgtgatgtt ccgctgatgg aaggaatcgg cattcaaaag atcagctttt ggaaagagag
    17641 aaacagcgac atcatgattt tgcgaatgaa taaataacgc gtttgatgaa gctacgctcc
    17701 gattcaatga ctgcatcacc cctctgatgg ccgatgccag tcgccgattt ggttacgact
    17761 cacccaaaaa aattcatatc cgttacgatg aaactttgat gtgaaggggc tttgacttgc
    17821 tgatcaaatg acgtgaagat gttctttaat cgattgcctg ctgatggatc cttcacgaag
    17881 gacgaccggc ggattcaacg taacatcaat cagagtggat gatgccccgc ccggcgtttt
    17941 ccccccatcc agaattgcgt caattttacc gccgatttgt ccggcaactt ctgaagccgt
    18001 cgagcattcc ggcgcgccgg ataaattggc gctggtggcg gtcaggggtt ttttcatgtt
    18061 ttgaacgatt ttcagagcga ttggatgaga cgaaacacgc agaccgatct tgccggttcc
    18121 cgccgtcagc agcggcgaca cggtgtctga cgcctgaaaa acaatggtca gcgcacccgg
    18181 ccagaacgcg tcgatcagtt tttttgccgt ttccggtaca tccgtaacca gcatattgat
    18241 atcttccggc tttgcgatga ttaaagagac gggattttta aaattccggc ctttgacggc
    18301 gaagatttta ccgatggctt cttcatgggt ggcatcgact cccagaccgt aaaacgtttc
    18361 tgtcggatag gcaatgatgc cgccgtccgt caggattgtt acagcgcgat tgatcattgc
    18421 gtcttcggaa atttccgttc ccggcttcag gatttccggc atgtgagatt ttcctgcttt
    18481 ttctccacgt ctttggctat tttccggatg taatcgcgga gtttcttttc caaagtcttg
    18541 tcgtcaagcg caagcatacg aacggcaaac agtgcggcgt ttttggcacc ggcttttccg
    18601 atagccatgg tagccacggg aatgcctccg ggcatttgca cggtggaaag aagcgcatca
    18661 agtccgttta aagcggtagc gtcaatcgga acgccaatga caggcagggg cgtctgagag
    18721 gcaatcacac cggccagatg agccgccgct ccggcgccga caataatcac cttgatgccg
    18781 cgcccggctg cagctgaagc aaattgtgtt gtgcgaagcg gcgtgcgatg tgccgacgtc
    18841 aaaatcagtt catgagcgat gccgaattct tccagaacct ttgcggcttc ggtcataacg
    18901 ggaagatccg aatcgctgcc cattaccaca ctgatcaagg cattcctctt gacgattttt
    18961 gctttcatgc aggctccttt attggctgat attattgctt tttattattt tttatatcta
    19021 acgcatcggg ccttcatttg caatagttat tataaaatcg atctttttcc caaatccagt
    19081 tgacataaca atatgatctt catatactcc cgccaaaatt tcttacccgc actcaaggag
    19141 agatgtaaaa aatgaaaaat aatttatatg aatttctggc cggcaacgat tatccgggac
    19201 gcggtatttg tatcgggatg acgcccggcg gcaaagccgt gattgcctac ttcatcatgg
    19261 gcagaagcac caacagccgt aaccgtgtat ttgatccgat cgaagggggc atccgcacca
    19321 aggctgccga cccgtccaaa atgaccgatc cgcatctgat tatttacaat ccggtactga
    19381 cctttgacaa aacaaccatc gtgaccaacg gtgaccagac caatacaatc tatgatttta
    19441 tgtcgcaaaa caaatttccg ggctttcctt tcgaagctgc tttgcatacc cgcacgtttg
    19501 aagacgacgg tcccaattgg acgccgcgta tttccggtat ggtggatatg agaacaggcg
    19561 cttacaagat caatatttta aaaagttgcg acggggacga gaaaagcgtg cagcgtttca
    19621 ccttcgatta tgttcagccg ctgcccggcg agggacactt tatcagcacc tataaatgca
    19681 acggcacgcc gattcccagc tttcagggcg aaccgatgcg cgtcacgatc gatgaagaag
    19741 atcccgaccg gtatgctgca aaactctgga aagcattaaa tgaagaaaat aaggtcagtc
    19801 tttttgtgcg cgccatcgat ctgacgacgc aaacctgtaa agacgtcatc atcaacaaat
    19861 ataaagcagt ggaggaatga tcatgaacga aatacaactg aaatacggct gcaatcccaa
    19921 tcagaaaccg gcgagaatct tcatggcgga cggcagccat ctgcctgtgg aagtgttgaa
    19981 tggcaaaccc ggctatatca actttttgga cgccttcaac agctggcagt tggtcaaaga
    20041 actcaaggaa aataccggta tggtggcggc agcctccttt aaacatgtat ctcccgcagg
    20101 cgctgctctg ggttatcctt tggacagagt gttgcgcaaa atgtatcaca tcgattccaa
    20161 aataaagctc tccccgctgg cctgtgccta cgcgcgcgcc cgcggcgccg accgcatgag
    20221 cagtttcggc gactggattg cgctttccga tgtgtgcgac gtgtccacgg ccaaaataat
    20281 caaaaacgaa gttactgacg gaatcatcgc gccgggttat gagccggaag ctctggaaat
    20341 cttgaaatcc aaaaagagcg gcaattacaa cgtggtgaag atcaatcccg actacgtgcc
    20401 ggtttccctg gaacacaagc aggtttacgg catcacgttc gagcagggac gaaacgactt
    20461 gaagatcgac agccgcatgc tggaaaacat tgtaaccgaa aacaaaacac tgaccgacgc
    20521 gcagaaacgc gatctcgtac ttgcgttgat taccctgaaa tacacgcagt ccaacagtgt
    20581 ttgctatgta cgggacggcc aggttatcgg tgtcggcgcc ggacagcaga gccgtatcca
    20641 ctgcaccaga ctggccggac agaaagccga cctttggcag ttgcgccata tgccaaaggt
    20701 gctgaatctg cctttccgcg acgatgttgc caaacccaac cgtgacaacg cgattgatgt
    20761 ttatctgggc gaaacaccgg aagatgtcac cggcgatgat gtctggggcc aaacatttac
    20821 gaagcagccc aaacccttaa ccaaagctca gaagcagaaa tggctcagca aggtgaccgg
    20881 cgtgtgcctg ggcagcgacg cgttcttccc cttcggcgac aacattgaac gcgcccgccg
    20941 cagcggcgta accgccattg tcgaacccgg cggctccatc cgcgaccagc aggttattga
    21001 cacctgcaac aaatacggga ttgtgatggc gttttgcggc ctgcgcctgt tccaccatta
    21061 atatttctgt catgctggcg caggaagccg ggaataagaa acatattgac caaaatcagg
    21121 acgggcgggt tcaaaagact cacccgaacg tatacagcag actgaaagat cagaagtcaa
    21181 aaatacgtct gcatgcgcat tataattcta agaagaaagc ctgaaaaaat ttctgtacca
    21241 cgggatttta tgcgatccat tattcaatag ggatgaataa tttttcatta caaaagcgat
    21301 attgtatttt tccagcagat gcttcagctc tgtctccggc tttattctga atcttgaata
    21361 atcgcggccg acaggtttgg tgtgtatggc ggtaatatcg tcaataatgg ctattttatc
    21421 tttcggggaa ttcaaccggt gagaccaggt tgcatcgagg ccccagccgg attgggaaag
    21481 atcgaaatca ttgcggagag acaagagggt cttgacatcg aacagaggca tcatgacttc
    21541 gacaaaattt gtaaatctta acgtgtttcc aaccctcggt ttcgttatcg cgtgcgtcac
    21601 atcgccgtgt aaggatatta aagcgggctg acaaatctgt aaagaatatg tcctggctgt
    21661 ctcaaaaaga cgattcaagt catccgctga tatggaaata tcgtcatcgg gaagccaaac
    21721 atagtcatat tgggatatga gttctaaatt cgtttcaata aaggatttga ttaaaggaaa
    21781 cttctgacca ttttgtttga tgcaaatcat gacatctttt gaatagtcga aaaaaatcaa
    21841 atcactgtta ccgtaataaa tgaggatcaa atcaaaggcg gggttgccgc ttttccattg
    21901 tttgtgcagg gagctgtctc cgacagcggc tataaccaaa tttttgttct tcatcattca
    21961 atcgggccaa gaatattggg cagaataaca gccgtttgcc gtaagcggca acagctcggg
    22021 tgatcttctg atacggcata tggcatttaa tattttcccg ctctcaacat cttatcaata
    22081 tggataagca cgttcttctc gcggggacga atatatgaaa cggtgccctg tatgtcaagg
    22141 cttctgttgg gtggtaaatt cccggtgctg aattcattct gtgaatgctt gagttttaaa
    22201 gacgtgaagt atgggtgata acaggtgtaa agaaagggcg tctggccttt gtttttcgaa
    22261 tttattttct aaataagtaa agatggcatt gtaaaatttc ttccagaatc aatctgtcga
    22321 tcttcgacaa gggcaatttt tttaaatcag cgggcgaggt ccatcgccag ttttggcacc
    22381 caagcggttt gggcgtgcct ttgagcgaat gacactcgta agcatgaagg gttaagcgga
    22441 agtgcgaata ggtgtgattc accgagagca gatgtttccc cggttggatg gaaattccca
    22501 gttcttcttt tacgttgtgc ttcaaacttt tttccatgtt ctcgcctttt tcaacaaaac
    22561 cgccgggtag tttccacaaa gatgccagca aaccggatgc agagcgctga acaaccagta
    22621 acatgccttt tgcattacgg atgacggctg ctgctgcctg tcgatgcggg attttagaag
    22681 ctttctttgt taaaggcaga atgttttgta aattccggag ccgtgcccgg cataggttgc
    22741 ctatgggaca gcgggaacaa tcgggatttt tagatttaca aattgtcgct cccaagtcca
    22801 tcagggcctg gttgaaatca cccggatggt tgacaggaat cagcgaagcg gcaagcttct
    22861 gcaattgctt ttgttcctgc gcgtcgtcca acggtttgcg gatggcaaat acacggcaca
    22921 aaatccggcg gacatttcca tccacggcag gaagtgcctg tttataagcg atgcttaaga
    22981 tggcaccggc tgagtattgg ccgattccgg ggagagtttt tatttcttcc agattatcgg
    23041 gaatccggcc accgaatatt tccacaatta ctttggcggc agcatgcaga tttcgggcac
    23101 gtgaataata ccccagattt tcccatgcct tgagcacatc ctgcaaggga gcactagcca
    23161 gtgaggaaac ggaggggaat gtttttagaa aacgatgata gtagggaagc accgtatcca
    23221 cctgggtttg ctgaagcata atttccgaaa tccagatacg gtaaggatcg
//
LOCUS       DAOOLB010000030        22727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_2175_length_22727_cov_27.332481, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000030 DAOOLB010000000
VERSION     DAOOLB010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 22727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22727
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2175_length_22727_cov_27.332481"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="PLF77_05515"
     CDS             complement(<1..979)
                     /locus_tag="PLF77_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPC08349.1"
                     /translation="MMKTDSTGHASEKPCLEENLVKNILPLSTLDNEIRPGGNPIEIL
                     LFYKREVSVLNIQEGFRKAVEHYNLFSSRLIMMDHDQFALQYCSDGAVINILPALPQT
                     HDNVDIEHVRKMMTHVKTLPGEPLFALTVVPVRDGYLGGFSFSHAIGDGVSLILFLFS
                     LGLIMAGEHFPLPSSQRLFTGSPLSVDQIDKSFILPLHALNKKIQDRIRQANMQTYTT
                     REYFSEEQLKAIKYQAKSAGEHYEISNNQIMTSLLLKKYHHHLLPDADRIRLRSPVNL
                     REIHPDIDPCYIGNALFVSFTEFEKDEIDRMPVYEIACRFKKSIMQMRNE"
     gene            complement(1174..1791)
                     /locus_tag="PLF77_05520"
     CDS             complement(1174..1791)
                     /locus_tag="PLF77_05520"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HPC08350.1"
                     /translation="MALGIFCDIADDFKHGKNFHCGSFVKIEKNVVIGDDVSIGDYVL
                     LKSGVRIGNHVEIKEYVMLMSGSRISDRTSIGNYTKIGENAVIGVRCQFFSFCEIRDA
                     CKIGNNVLMGSRGTLSAHAVVEDDVVMKYSFVVTDTPDLDREEEKKVGVIKKGSKFGT
                     NVCIMPGVTIGEDAEIGACSQVRNDVPANEVWYGNPARFMKKRSP"
     gene            complement(1816..2640)
                     /locus_tag="PLF77_05525"
     CDS             complement(1816..2640)
                     /locus_tag="PLF77_05525"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HPC08351.1"
                     /translation="MKIIDSRVRLRTEQLLKIFNDDHTKYYSDYVHLYKMTSRLSPMP
                     VEELIGIAGGHDIEKMMVYGLSAEDNDHVLNLARRFKEIIPVAGVSSDKGIRHALQDI
                     QKYCDAGAAALFISPFSLNCPLNDRLYYPLYGICELSRKAVIIHGSIHFWRGAYMWRG
                     QPQYIDEIAVDFPELKIIVSHGGNGFGPSILAVAQRHPNVYLEFSALNPSYMAPEFLY
                     AANTYLKNKCIFGTDYPLVDFHVAIGRWKKVLREDVQDLFFYRNVLDALHKEPVSY"
     gene            complement(2627..3895)
                     /locus_tag="PLF77_05530"
     CDS             complement(2627..3895)
                     /locus_tag="PLF77_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPC08352.1"
                     /translation="MESSETKNADYGFRIKGNPVLLYKDRSLAADESTHQMEKALRQM
                     SMEYYVLDRCMQCGICSSACPNALLKKENIFTPRTFIEKTRLGLLDLNSDDLWSCTHC
                     GSCRIACPFDIPLPDVMAQLRHLVVEKGAGYVPVSIKRSISSIASCGNPWKEGAMDRK
                     KWLCATGLPETDGYDERRVCVFLGCLAGYDRRARQTAEAVIRILQTVGVPFKILADEE
                     VCCGDTVRRIGDFSTAEKVEKLNRKNIIKNNVETMYVLSPHCFSAFKDVRRSDAMENI
                     RTDLLIELIYRLFIGGAFHFTARIEKKVTFHDPCFLSKHLGIINQPREILRGIPGIEI
                     LEMKHHGKRSLCCGGGGGGIWRDAVKGERLSEIRLDEAMETGAQTVVTSCPYCLYMLE
                     DARRGDEKYQNLEIVDLFEFIGEGISHENH"
     gene            complement(3898..4959)
                     /locus_tag="PLF77_05535"
     CDS             complement(3898..4959)
                     /locus_tag="PLF77_05535"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPC08353.1"
                     /translation="MMNKKMMKSKVLIIGGGVTGLVAAVSLDELGIDSIIVEKEPELG
                     GKVKKYSRVFPDFRSGKTITQNLIAKIQKSSRVRSVVSTSVASVERVNGWYVVTDSND
                     TKYKVKAIILACGFDVFDARCQDEYGYGVYPNVVNSLELEALLEAQELQGCSLVRPSD
                     GNPANRIAIIFCVGSRSRRLGNPYCSRICCSYSTKQAIEIIEKNSDASVTCFYMDIRT
                     YDRGFEEMYQYAQELGVKYVRGRVSECSALPNGDIQVRAENTLLSKPVQGIFDIVSLS
                     VGMMPCNDAAELAELFGIERSDDGFFTRKDRFFFQHDTVKEGIFLAGSVTGLKPIKDC
                     LLEGTSVSSRVAAYLNRNK"
     gene            complement(4949..5998)
                     /locus_tag="PLF77_05540"
     CDS             complement(4949..5998)
                     /locus_tag="PLF77_05540"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HPC08354.1"
                     /translation="MVNYNQEGRQKLWGKYSKDIPGDHYFLTFSCILGSFYPASETMV
                     PGIYDLLDVNWKTQYESKSGWSCCTAIGYHGDIMPIEATLLTVARLWSIAQDAGMNVI
                     TPTCVTSFGTYCECKELLEQEPDLRKKIKRLLKETCDREFEIPEYLVHVSDIYYHHRE
                     KIKKHFQYSLTEAKTGRPLQVVDHIGCHYSKLFADKYSLGGSEYCEVLSGMVRAWGGE
                     NIDYPERRHCCGMGFRQCMIPPNRGATISNVYKKIKSMAPFNPDLILTNCPGCQVFMD
                     KEQWAIHEVTGEKYFIPVLTYQELAGLLLGWDPYDVVGIDAHTVPVEPFLDKIGIVYD
                     RKKSMHLEKTGSYDE"
     gene            complement(6005..6964)
                     /locus_tag="PLF77_05545"
     CDS             complement(6005..6964)
                     /locus_tag="PLF77_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPC08355.1"
                     /translation="MKDQYQFCNIDEKPGKKNWDDIHRHLEELSSDEIIDLYYRLRDA
                     CTVSGGEVCYPNIQYLHATTFEREKDRREQRQWIQDKKGLSDVVEWYREARDKSCVWW
                     LENHVIAKHSFSSCMSCGACTALCPAAEFYDFSPREMMTLVQEKNEEDIIEFLKSEKL
                     WYCHQCGSCKTKCPRQNSPFGIISSLRQLSQLKGYHIESIRGRQQYYARHLWGGNLWN
                     RACTLYFRNPLPDVHQDFGPRFATYFRHKDEFMKKIGAHPDMDGPYSGRKIAPATLDE
                     LRRLWFAGGALFLWSLIEEAAQKQARDMNVSIDEYHLKVKTEG"
     gene            7199..7978
                     /locus_tag="PLF77_05550"
     CDS             7199..7978
                     /locus_tag="PLF77_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPC08356.1"
                     /translation="MTAEIVCCIITGFAAGLATVLSSISAAIIISPVLITFLDVPAYA
                     AVGIALAADIFSSTASAIMYGKNRHVNFRGSYYLLMTTLVFTALGSYIGAHTPDNAIG
                     YGLIFSTTFLGGKFLVAPVKSPLESIPENLNRKHVWTTLLSGAVIGLISGIFGIGGGL
                     MILIALVFLLQYELKTALGTSLYIMAFTALIGAFSHFIIGGVPDLAILTACICSATSG
                     AWFSSMFANRTSAKILNVTVGILLLGIGLAMLFKQCHGLIK"
     gene            complement(8027..9316)
                     /locus_tag="PLF77_05555"
     CDS             complement(8027..9316)
                     /locus_tag="PLF77_05555"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPC08357.1"
                     /translation="MDARIPVEKAARVFRIARESGIISSVDTAVIFHDLGRMEGRIKR
                     LQKLFPESTLHALAIKANPLVALLKRAVAAGAGMEAASIEEVHLALAAGCPPEKIVFD
                     SPAKTDAELAFALKKRIHINADGLQELKRIEEMIAAGSDHGRVGLRVNPLVGAGSIAM
                     TSVAGKNSHFGVSLDDKHQDVIMKAFASCRWLCGLHSHVGSQGCELSMLTAAADRIWK
                     FRQTVHQEIGREQINVMDIGGGAPALYSDAESRFSLEDYAEALKREVPGLFEKDIRLV
                     TEFGRSVLANCGFAVARVESVKQISDRRIAVVHLGADFLLRPVYQPEFWHHDCSLLDT
                     RGYVTSGDPASWSLAGPLCFSGDCFAGGVMLPPVHSGDHIVIRDTGAYTLSLWSRHCS
                     RAIPLIIGYDEADDRNIRFVTLRRRETVDDIVSFWSP"
     gene            complement(9334..10953)
                     /locus_tag="PLF77_05560"
     CDS             complement(9334..10953)
                     /locus_tag="PLF77_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08358.1"
                     /translation="MLKVIKSFAADPVSLELRTEALLEIGADSRSQTGENGLTPYGTT
                     PGPRNAVPLGSCTASSPLQRVFDAAVTQHAQLRDAHLQGELLQHIRHRYCRIRMAVRR
                     HLRISRQDVRIALTPSGTDAELLVLALALGDRSRPLCNIVVGPQEIGSGSSLAAAGRY
                     FDAVVPHGPRQVKDTPISSAIARLVHVEMIPIRDANGCVREPGDIDAETDALIHEALA
                     KRERVLIHIVAHSKTGIFAPGISAVRKAQRKYPRDISVVVDAAQGRFSRKSLNAFLKN
                     GFHVLFTGSKFYGGPAFSGALMVPGRYDSMIRKLPPLPVEFGHYFSRWDLPEEWSSLS
                     SELPHDYNLGLLLRWFAALEAIKAYYSVPGRNRLAILKRFENGVLKECGAYPCFKCDR
                     VEPVQFPKGKERLLESRTSVFSFFMRVLTASGELRYPENDELKAVCKWINEDISRFAA
                     GESSTLKKVLASTFNIGQPVSIGSEKHPAVLRVALGAALVTDVGLHRSCGAVRGERLA
                     WLDEQIRNVFLKLDWISRNYVLLVQDKEMPR"
     gene            complement(11282..12514)
                     /locus_tag="PLF77_05565"
     CDS             complement(11282..12514)
                     /locus_tag="PLF77_05565"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPC08359.1"
                     /translation="MILKKEQRSYLEENLGKTILPLSLLENKTLIGSNPLEVLLFYKK
                     DWTYEQFGEAFLKAAHYFNLFSSRLIRMDEDRYALHYCADGVEFNFLPPMDATSDSVD
                     IEDIRKKIKKVKTLPGGPLLCLTGIAVKDGKFGGISCSHVIADGFSLILFLHLWGCII
                     EGNELPLPSPQRLFKGEPVKSERIDPLFFPPLSDLNDEIQNRYQSSHARIYQKREYFS
                     DEFLREHKQKAKSTHEKYEISDNQIMVSCLLKKYHRHILPDTDKVRLRHPVNLREIHP
                     DIDPMYIGNAFFFSWTEFTKDEIDQLSIYEIAYRLKESIRSARDQNFIQDISYVSKYG
                     IEFKPDMFNHYNATDMDTDILSTNLAHMNDLASLGIGPDKGRFLYVDLPIQNSFIMLK
                     EKSGEVFAQITSRYPLPS"
     gene            complement(12683..13918)
                     /locus_tag="PLF77_05570"
     CDS             complement(12683..13918)
                     /locus_tag="PLF77_05570"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPC08360.1"
                     /translation="MMKLGEKQMLYLKENLGKTILPLSIFENEIRPGTKPMEIVLFYK
                     KRTSLLKIKESLFRAIEYYNLFSSRLIMMDDNKFALQHCIDGVEYNILPSMNTPLDHI
                     AVDEMKKKMAHVRTLPGEPLFAVTGMPIKDGLLGGISCSEAIADVFSCILFLYTWKCI
                     IEDKSFPLPSTQRLFKGKPVASDAIDKIFTPPLSALSEKIQSRVRASEIKLYSKREYF
                     SDEFFDEHKNKAKLENEKYIISSNQIMTAFLLKKYHDQLLPDTDRIVLRSPVNLRDVH
                     PDIDSMYIGNVFFDNKTEFTRHEINTMSIYEIAYRLKESITKTTNENYVKNISYLSKY
                     GIEFKPDIFKKYRSLDVNTDIVSSNLVLLNDPESLGLSKLFNILHLNSTVQTSFMMYK
                     EKKGRIFTQITSMYPFRLH"
     gene            complement(14621..15787)
                     /locus_tag="PLF77_05575"
     CDS             complement(14621..15787)
                     /locus_tag="PLF77_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HPC08361.1"
                     /translation="MKERRYDLDWLRVIVMLLVFMFHCSRFFGGGTWLLKNAEESIVA
                     LVYVSLIDMWVMPFFFFLSGIGSWYALAYLTNGQYVQERIKRILIPLYTVGFFILLPP
                     QFYFEIYSNAGFTGTFWESIPLYLDREGHFSIGSPPDLLMLSFITHLWFLGYLFLVSL
                     VSLPFMCYLRSGHGLRFIDKLAGWCTRPGGIFIFIIPVLFMRVGLRSFFLGQFTWADF
                     FEYMVYFIIGYLFVADSRFMQSIKKYGWFGLILGLACWGGQGIFVLGLGYNYPDGESF
                     SLTYVLFETIMSVSRWSWIVIVLSLGAKYLNFNHKVLTYSNEAVLPFYLLHQPVILGV
                     GWFVIQLNIGMGLKFLIITLISFVLIMAVYDLFVRRFNVVRFFFGMRPIKKSPA"
     gene            complement(16095..17327)
                     /locus_tag="PLF77_05580"
     CDS             complement(16095..17327)
                     /locus_tag="PLF77_05580"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPC08362.1"
                     /translation="MKLNEEQRAYLENNLGNTILPLSIFENEDWPGNEPIEVLLFYKK
                     EWSNSEIQESFLKVTEHYNLFSSRLIMIDDNKFALHYCTDGVKFTFLPPVDDTYDNIN
                     IDEIRKQMVKVKTLPGEPLLSLTVISAKDGKFGGVSCSHTIGDGISLILFLMTWGCII
                     EGKSFPLPSPQRLFKGKPVASDTIDKIFTPPLSALSEKIQNRVKASEVKLYSKREYFS
                     DEFFDEHKNKAKLENEKYIISSNQIMTAFLLKKYHDQVLPDTDRIRIKIPIDLREIYP
                     GIDSMYIGNAVFDNFTEFTKDEINKKSIYEIAYCLKKSVINARNENYIKEISYLSEYG
                     ITFKEELFKTRPAFANMDTDIVSSNMAHMNDLGALGIGEDKGHFVYIGIPTQTSFVML
                     KEKSGQIWAQITSRYPLE"
     gene            complement(17539..18198)
                     /locus_tag="PLF77_05585"
     CDS             complement(17539..18198)
                     /locus_tag="PLF77_05585"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPC08363.1"
                     /translation="MRNEAVIFDMDGVLIDSEPLHISAAIALLKKFHVDTSEGYHNKY
                     VGCSDPVKWQKIKDEFDIKSSLREILDMSVSTKLELLRQTDCNPIEGIPELLKSLYDR
                     SIPVAVASSSPGRFIEEVIDKIKIEKYIRTWVSGENIEKSKPEPDIFLKTAELLQVNP
                     RGCVVIEDSKNGVIAAKKAGMRCIGFKNMNSGSQDLSKADLVVDKIQDIDLCRLLSME
                     Y"
     gene            18451..19800
                     /locus_tag="PLF77_05590"
     CDS             18451..19800
                     /locus_tag="PLF77_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPC08364.1"
                     /translation="MEKNMLHHKSFTQIHNRPAEILEKLIRFNTTNPPGNEAACINYI
                     RNLLNDAGIQSTIIAKKPERPNLIAHIDGRGKANPILLYGHVDVVGTDNQQWDIPPFD
                     GKIIDGVVWGRGALDMKGGIAMMLAALLRAKAQNFKPAGNVLLAIVSDEEAGGDLGTK
                     FLVEEHPELFKDIRYAIGEFGGFNVNLDNKRFYPITISEKQICWMKATIRGQGGHGSM
                     PVRGGAMMKLSDFLRRLEKNDLPVHVTVPSKMMVEAVADALGGIKGLVFSQLSNPWMT
                     GIILNLLGASGKGFYPLFHNTVSPTILHGSASINVIPSEISVDLDGRLLPGYKPEDMM
                     NELRPLVGDNVRFELIKYDAGPAKPDLGLFGVLSQILHEADPEGITVPFMLSGVTDGR
                     FLSKLGIQTYGFLPMLLPEDFDFSATIHAANERVPVRAIEFGADAMFKVLERFGQDE"
     gene            complement(19832..20737)
                     /locus_tag="PLF77_05595"
     CDS             complement(19832..20737)
                     /locus_tag="PLF77_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08365.1"
                     /translation="MNKKNQKVPFHIKGSKVRVKKFRGTNFIFEEVNRPCDLKDSDFV
                     DKESLFENIRQVARKTLREEVAINIPKYFSESDLMYIYSDMNHKVVAMSVCGSMFDQG
                     DFVMIIWLTMCLEEYRKNGLLRALIPKMFLDYLKKYNREMGYTGIKRIIPFFRTAYMF
                     GRAINPIIYHAVFASPMNISPPIDKNGDINVSHVSEKEINIRKNYLRKLGYTEDKINE
                     NLFVFRHILTDEKENTITHASMPLCPEAHVNKYFKENLGLEKGNALLILIYCRPLPAM
                     VIQFPKVFFKNTWRAIRDRLLALRK"
     gene            complement(20830..20906)
                     /locus_tag="PLF77_05600"
     tRNA            complement(20830..20906)
                     /locus_tag="PLF77_05600"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20870..20872),aa:Arg,seq:ccg)
     gene            complement(21001..22554)
                     /locus_tag="PLF77_05605"
     CDS             complement(21001..22554)
                     /locus_tag="PLF77_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPC08366.1"
                     /translation="MRENLFKDALLNPSVFPVTWELVPGRGARETAQEKALALAKQAS
                     IGGKICALTLTDNPGGTPAMSADFMGAEIVKFGIEPLVHFTCKDKNRNQIESQLYALD
                     RSGVRNLLVMSGDYPVSGFHGRPAPVFDLDPIHVLQLISEMNAGLEYPGIKGVVRHQA
                     SDFFPGAVISPFKATEAEQMTQYFKLKKKIAAGAQFIITQLGYDARKFHEVLLFMRQH
                     GFNIPLVGNIYILPYGAARMMNRNELPGSVVTDKLLAEIDRERNDSDKGEGARILRAA
                     RLYAILKGMGYSGVHIGGHNIKYEQVENIINQGEALVPQWQDLVGYFDYPLSDGFYYY
                     ERDPVTGLNKETPVRRQNRPLDSNVEWTYGFSRFFHKLMFEPGKKLYGLMKTVSEKIS
                     GTGMAKIFHNLEHVAKVVIYDCKDCGDCALLDVAYVCPMSQCPKNQRNGPCGGSFKGW
                     CEVYPGKKHCVYVRAYVRLKKYGEAEHLEHKIVPPCNWDLYQTSSWINFYLGKDHHSA
                     RSHQNDAEK"
BASE COUNT         6239 a         5514 c         4708 g         6266 t
ORIGIN      
        1 tttcatttct catctgcata atcgatttct taaatcgaca agcaatctca tacacgggca
       61 tgcggtctat ttcatctttt tcaaattcgg tgaagctcac aaataacgca ttgccgatat
      121 agcatggatc aatatcagga tgtatctctc tgagattcac cggacttctt aatcgaatcc
      181 tgtccgcatc gggcagcaga tgatgatgat acttcttcaa caagagcgat gtcataatct
      241 gattattaga aatctcgtag tgctcgcctg ctgattttgc ctgatacttg atggccttga
      301 gctgttcctc ggaaaaatat tccctcgttg tgtacgtttg catatttgcc tgcctgattc
      361 tatcttgaat tttcttattc aaggcgtgca acggcagaat aaacgattta tctatctgat
      421 caacactgag cggactgccc gtaaaaagcc tctgactcga cggcagaggg aaatgctcgc
      481 cagccatgat taaccccagt gaaaacagaa aaaggatcaa cgagactccg tcgccgatgg
      541 cgtgtgaaaa actgaagccg cctaaatatc cgtccctgac gggaacaacc gttaaagcaa
      601 aaagcggctc cccgggtaag gttttgacat gcgtcatcat ttttctgaca tgctcaatat
      661 caacgttgtc atgcgtctgg ggcagcgccg gtaaaatgtt aatgaccgcg ccatcggaac
      721 agtactgtaa tgcaaactgg tcgtgatcca tcatgatcag gcggctggaa aaaagattat
      781 aatgctcaac ggccttcctg aaaccttcct gaatgttcag aacagaaacc tctcgtttat
      841 aaaataacag gatttctatg ggattccctc ccggtctgat ttcattgtcc agggtcgaca
      901 acggaagaat atttttcacg agattttctt ccaggcaggg tttttctgat gcgtgaccgg
      961 tgctgtcagt tttcatcatc ctatctcttc tgaaataatt ttcacgttgc atccacgcaa
     1021 acaaaacggt gctgtgggcg gaagcacaga caacatctta tgacgacatc gccgttcggg
     1081 ctacaaacgc aaagacgacg tgtaaacgga tctttaacgg caagtcactt agatcaaccg
     1141 ataaaaacag acgacaacaa acaatgtatt tttctatgga ctacgtttct tcatgaaacg
     1201 cgccggattt ccataccata cttcattggc cggaacatcg tttcgcacct gggaacaggc
     1261 gcctatttcg gcatcttcac cgatggtcac tcccggcatg atacagacat tcgtgccgaa
     1321 tttggacccc ttctttatga cacccacttt cttttcctct tcccgatcaa gatcaggagt
     1381 gtccgttacg acaaaactgt atttcatgac cacgtcatct tcgacaaccg catgggcaga
     1441 aagcgtgccg cggctgccca tcagaacatt attgccgatt ttgcatgcat cgcgaatttc
     1501 gcaaaaggaa aagaattgac aacgaacacc gattacggcg ttttctccga tttttgtata
     1561 attaccgatg ctggtcctgt cgctgatcct gctgccggac atcagcataa catattcctt
     1621 gatttccacg tgatttccta ttctgacgcc ggatttcagc aaaacgtaat ccccgatgga
     1681 cacatcatct ccgatgacca cgtttttttc aattttcaca aacgatccgc aatgaaaatt
     1741 tttaccgtgc ttgaaatcat ccgcaatatc acaaaaaatt cccaatgcca ttttcatatc
     1801 tccggagaaa gcatttcaat aagaaaccgg ttctttatgt aaggcgtcca acacgttccg
     1861 gtaaaagaat aaatcctgca catcttcacg caacactttt ttccagcgac ctatcgccac
     1921 atgaaaatcc actaaaggat aatccgtccc gaaaatgcac ttgtttttca ggtaggtatt
     1981 ggcggcataa agaaattcag gcgccatata cgacggattc agtgcgctga attccagata
     2041 tacattggga tgccgctggg cgacggccag gatggacggc ccgaaaccat taccgccatg
     2101 ggaaacaata attttcaatt cggggaaatc aacggcaatt tcatcaatgt attgaggctg
     2161 cccgcgccac atatacgcac cccgccagaa atgtatagaa ccgtgaatga tcaccgcctt
     2221 tcgggatagt tcgcagatgc cgtaaagagg ataatacaga cggtcattca gcggacaatt
     2281 cagactgaat ggtgatatga atagtgcggc tgcacccgca tcgcaatatt tttgtatatc
     2341 ctgcagcgca tggcgaattc ccttgtcaga acttacgccg gcgacgggaa tgatttcttt
     2401 aaaccggcgc gccaaattca ggacatggtc attatcctcc gccgaaaggc cgtaaaccat
     2461 catcttttcg atgtcatggc ctccggcaat gccgatcagt tcttcaaccg gcatcggact
     2521 gagccgcgat gtcattttgt acaaatgaac atagtcactg taatattttg tgtgatcatc
     2581 gttgaaaatt ttaagaagct gctctgtgcg taatcgcact ctggaatcaa tgattttcat
     2641 ggcttattcc ttccccgatg aattcgaaaa gatccactat ctccaggttt tgatattttt
     2701 catccccccg tctggcatct tccagcatgt acagacaata ggggcaactg gttacaacgg
     2761 tttgagcgcc ggtttccata gcttcatcca gacgaatttc agacaaacgt tcgcccttta
     2821 ctgcatcccg ccagataccg ccgccacctc cgccgcaaca caaacttctt ttcccgtgat
     2881 gtttcatttc caaaatttct attccgggaa tccctctgag aatttcccgc ggctgattaa
     2941 tgatgcccag atgtttgctg agaaagcacg gatcatgaaa tgtaaccttc ttctcaattc
     3001 ttgcggtaaa atggaaagcc cccccgatga acaaccggta tatcagctca ataagcagat
     3061 cagtccggat gttttccatg gcgtcggaac ggcggacatc cttaaacgca ctgaaacaat
     3121 gaggagataa cacgtacatc gtctcaacat tgttttttat aatattcttt ctattcagtt
     3181 tttcaacctt ctccgccgta gaaaaatcac cgatcctgcg cacggtatcc ccgcaacaga
     3241 cttcttcatc agccagtatt ttaaaaggga ccccgaccgt ttgaagaatg cgtatgaccg
     3301 cctcggctgt ttggcgcgcc cgacgatcat atccggcaag acatcccaga aaaacacaaa
     3361 ccctcctctc atcatatccg tcggtttcag gcagtccggt agcgcaaagc catttctttc
     3421 tatccatggc gccctccttc cacggattgc cgcatgacgc gatcgaagaa atcgaacgct
     3481 ttatggacac ggggacataa ccggcaccct tttcaacaac cagatggcgc aactgtgcca
     3541 tcacgtccgg cagaggaatg tcgaaaggac atgcgatccg gcaactgccg caatgtgtac
     3601 acgaccagag atcatcgctg ttgaggtcca gcaatcccaa acgtgttttt tcaataaaag
     3661 tcctcggcgt aaaaatattt tcttttttca gcaaagcatt cgggcaggca ctggaacaga
     3721 ttccacattg catacaacgg tccagaacat aatattccat gctcatctgg cgcagggctt
     3781 tttccatctg atgcgttgat tcatcggccg ccagcgaacg gtctttgtaa agaagaaccg
     3841 gatttccttt tatcctgaaa ccgtagtcgg cgttttttgt ttcactgctt tccatgacta
     3901 tttgttcctg ttcagataag cagcgacccg actgcttacc gatgttcctt ccagcaggca
     3961 atccttgatc ggcttcagtc ctgttacgct gccggcaaga aaaatacctt ccttcaccgt
     4021 gtcatgctgg aagaaaaatc tgtctttcct ggtaaaaaag ccatcgtcgc tgcgttcaat
     4081 gccgaagagt tcggcaagtt cggccgcatc gttgcacggc atcatgccca cggataagga
     4141 tacaatatcg aaaattccct gtaccggttt gctcaacagg gtgttttccg cccgtacctg
     4201 aatatctcca ttgggcagag cgctgcattc cgagacgcgt ccacgaacgt atttcacgcc
     4261 aagttcctga gcgtactgat acatttcttc aaaacctcgg tcataggttc tgatatccat
     4321 ataaaaacag gtgacgctgg cgtcggaatt cttttcgatg atttcgatag cctgttttgt
     4381 actgtatgag cagcagatcc tcgaacaata gggattgccc agccgtctgc tgcgcgatcc
     4441 gacacaaaag atgatggcga tcctgttggc cggatttccg tccgagggcc tcactaagga
     4501 acatccttga agctcctggg cttccagcaa tgcttccagt tccagcgaat tgacaacatt
     4561 aggatagaca ccgtatccat actcgtcctg acagcgcgca tcgaaaacgt caaaaccgca
     4621 ggccaatatg atcgccttga ctttatattt agtgtcattg gaatccgtca caacatacca
     4681 tccgttgact ctttccaccg acgcaacgga tgtggaaaca acagagcgga cacgtgatga
     4741 cttttgaatt tttgcaatca ggttctgagt aatcgtttta ccgctgcgaa aatccggaaa
     4801 gaccctggaa tatttcttaa cttttccacc caattctggt tctttttcaa caatgatcga
     4861 atcaatgccc aattcatcca gagaaacggc cgcaaccagt ccggtcacgc cgccgccaat
     4921 gatcagaacc ttgcttttca tcattttctt attcatcata ggagccggtt ttttccagat
     4981 gcatggattt ttttcggtca tacacaatgc ctattttatc caggaacggt tcaaccggca
     5041 ccgtatgggc atcaataccc acaacatcat acggatccca gcccaacaga agaccggcca
     5101 gttcctggta ggtcagaaca ggaataaaat atttttctcc cgttacttca tggatcgccc
     5161 attgttcctt atccataaac acctgacatc cgggacaatt ggttaaaatc aaatcggggt
     5221 taaaaggagc catgcttttt atctttttat aaacattgga tatggtcgct ccgcgattgg
     5281 gcggtatcat gcactgccgg aatcccatac cgcaacaatg acgacgctcc gggtagtcga
     5341 tattttcgcc tccccaggcg cgcaccattc ccgacagaac ctcgcaatat tcggaaccgc
     5401 ccagggaata tttgtccgca aaaagtttac tgtaatgaca gccgatatga tccacaacct
     5461 gcaagggccg acccgtcttc gcttccgtca gactatattg aaagtgtttt tttatttttt
     5521 cgcgatgatg gtaatagata tcgctgacat ggaccaggta ttcgggaatc tcaaattcac
     5581 gatcacacgt ttccttcaac aatcttttga ttttttttct caaatccggt tcctgttcca
     5641 ggagttcctt gcattcacaa taagtgccga aagacgtcac acacgtcggc gtaatgacgt
     5701 tcatgccggc gtcctgggca atggaccaga gccgggccac tgtcaggagc gtcgcttcaa
     5761 tgggcataat gtcaccgtga taaccgatgg ccgtgcagca ggaccagccc gatttggatt
     5821 cgtactgcgt tttccagttc acatccagaa gatcataaat tccgggcacc atggtttccg
     5881 atgcggggta aaaactgccc aaaatacaac tgaacgtgag gaaataatgg tcacccggta
     5941 tatccttgga atattttccc cagagttttt ggcgtccttc ttgattgtaa ttgaccatga
     6001 atcttcagcc ttcggttttt actttcagat gatattcgtc aatagataca ttcatgtccc
     6061 gcgcctgttt ttgagcggcc tcttcaatta acgaccacaa aaacaacgcg cctccggcga
     6121 accataacct tctcaattca tccagcgtgg cgggagcaat ctttcttccc gagtagggac
     6181 cgtccatatc gggatgcgcc ccaatctttt tcataaattc gtccttgtga cggaagtatg
     6241 ttgcaaaccg gggaccgaaa tcctgatgga cgtcaggaag gggatttcta aaatacagcg
     6301 tgcaggctct gttccagaga ttacctcccc agagatggcg ggcataatac tgttgtctgc
     6361 cccgaatgct ttcaatatga taccctttca gctgactcaa ctgacgcagg gaagaaatga
     6421 ttccaaaagg gctgttctgg cgcggacatt ttgttttgca ggagccgcac tgatgacaat
     6481 accagagctt ttctgatttc aaaaattcga taatatcttc ttcatttttt tcctgaacca
     6541 gtgtcatcat ttcccgcgga ctgaaatcat aaaactccgc cgccggacaa agcgccgtgc
     6601 acgcgccaca gctcatgcaa ctggaaaagg aatgttttgc aatgacgtga ttttccagcc
     6661 accagacaca ggatttatcc ctggcttcac gataccattc cactacgtca gaaaggcctt
     6721 ttttatcttg aatccattgc ctctgctcac ggcgatcctt ctccctctca aaagttgtcg
     6781 catgcagata ttgtatgtta ggatagcaga cttctccgcc ggaaacggtg cacgcatcac
     6841 gaagacgata gtataaatct atgatttcat cagaggacaa ttcctccaaa tgacgatgga
     6901 tgtcatccca attttttttc cccggctttt catcaatatt acaaaattga tactggtctt
     6961 tcatatccgg tttccttccc gacagcttgt ccgacagaat atacccgtct gaactttttt
     7021 ctgaaattta attttaataa tgtattatag aaaaaaccat ataacaagaa gtaattttga
     7081 cgacacaaaa cggttttata aaaaagggtt gaagaatgat gccgcatata agcgcaaccc
     7141 atatgcttat tatcacttac aaattgacca ctataagcaa gcccggcagg ctttatctat
     7201 gacggcagaa atcgtttgct gtatcataac aggttttgcc gcaggtctgg ccacagtcct
     7261 cagcagcatt tcggctgcaa ttattatttc accggtgctc attacttttt tagacgttcc
     7321 cgcttatgca gcagtcggaa tcgcactggc tgccgatatt ttttcttcaa cggcctccgc
     7381 cattatgtac gggaaaaacc gccatgtaaa tttccgggga agttattatt tattgatgac
     7441 cacacttgtc ttcaccgccc tcggcagcta tatcggagcc catacccccg acaatgccat
     7501 tggatatggc ttgattttta gcacaacgtt tctgggcggc aaattcctgg tggcgcctgt
     7561 aaagagtccc ctcgaaagca tacccgaaaa cttaaaccgc aagcacgtat ggacgaccct
     7621 tttgagcgga gctgtcatcg ggctcatcag cggaatattc ggcatcggcg gcggtctgat
     7681 gattttgatt gctctggtgt tcctgcttca atatgaactt aagacggcgc tgggaaccag
     7741 tctttatatt atggccttta cagcattaat cggagctttt tctcatttca tcatcggagg
     7801 cgtccctgac ctcgccatcc tgacagcctg catctgctcg gcgacgtcag gcgcctggtt
     7861 ttcttccatg ttcgccaaca ggacctccgc gaagatttta aatgttaccg tggggattct
     7921 tctgcttgga atcggtctgg ccatgttatt caagcaatgt cacggtttga tcaaataagc
     7981 attgaagggg aaaatcggga ttcagtcatc gtcgatggct tctacgtcac gggctccaga
     8041 acgagacgat atcgtcaacg gtttcacgac ggcgcagcgt cacaaaacgg atattccgat
     8101 catccgcttc atcataaccg atgatcagag gaattgcccg gctgcagtgt cgcgaccaca
     8161 ggctcaatgt gtatgctccg gtatcccgga taacaatatg gtcaccggaa tgaacaggcg
     8221 gcaacatcac gcctccggca aaacagtctc cggaaaagca gagaggaccc gccagcgacc
     8281 atgacgccgg atctcccgac gttacatagc cgcgggtatc cagcagcgag cagtcatgat
     8341 gccagaattc cggttggtaa accggccgca acaggaaatc cgcgccgaga tgcacgacgg
     8401 caatccgtcg gtccgatatt tgtttgacgg actcgactct ggctacggca aaaccgcaat
     8461 tggccagaac agaacggccg aattcggtga caagacgaat atccttctca aacagtccgg
     8521 gaacttcccg tttcagagct tcagcatagt cctcaagcga aaaacgactt tccgcatcag
     8581 agtataacgc cggcgcccct cctccgatat ccatcacgtt gatctgttcc cgtccgattt
     8641 cctgatgaac ggtttgccgg aatttccaaa tacggtccgc tgcggcggtc agcatcgata
     8701 attcgcagcc ttgagacccg acgtgggaat ggagtccaca aagccaccga caggacgcaa
     8761 aagccttcat gatgacatcc tgatgtttat catccagaga aacgccgaaa tgagaatttt
     8821 ttcccgccac gctggtcatg gcaatggacc ctgctccaac taggggattg acacgcaatc
     8881 ctacacgtcc atgatcgctg cccgcggcaa tcatctcttc gatacgcttc agttcctgca
     8941 aaccgtccgc gttaatgtgg attcgcttct tcagagcaaa agcaagttca gcgtctgttt
     9001 tggcgggaga atcaaaaaca atcttttccg gcgggcatcc ggcggccagg gcaagatgaa
     9061 cttcttcgat ggaggcggcc tccataccgg ctccggcggc aactgcgcgc ttcaataacg
     9121 cgaccagcgg attggccttg atagccaggg catgcagggt cgattcgggg aaaagtttct
     9181 gcaaccgctt gatgcggcct tccatccggc cgagatcatg aaaaataaca gccgtgtcca
     9241 ccgatgaaat gatgccggat tcacgggcaa tccggaaaac ccgtgctgcc ttttctacag
     9301 gaattcttgc atccatctca gcctctttaa aattcatcgg ggcatttcct tatcctggac
     9361 gagcagaaca taattccgcg aaatccagtc aagcttgaga aagacattac ggatttgttc
     9421 atcaagccag gccaaccgct ctccccgaac agcgccgcag gaacgatgaa gtccgacgtc
     9481 ggtaaccagc gccgcgccca gggcaactct caaaacagcc ggatgttttt cactcccgat
     9541 tgaaaccggc tgcccgatat tgaacgttga ggcgagcact tttttcagag ttgacgattc
     9601 tccggcggcg aagcgggaaa tatcttcatt gatccatttg caaactgctt taagctcgtc
     9661 gttttccgga taacgcagtt ctccggaagc agtcaatacg cgcataaaaa atgaaaacac
     9721 actcgttctc gactcgagaa gcctttcctt ccctttcgga aattgcacgg gttcaacccg
     9781 gtcacatttg aaacacggat atgcgccgca ttcctttaat acgccatttt caaagcgctt
     9841 taaaatggca agccgatttc gtcccggcac ggaatagtaa gctttgatag cctccaacgc
     9901 ggcaaaccag cgaagcagca ggccgaggtt atagtcgtgt ggaagttccg aagacaatga
     9961 agaccattcc tccggcaaat cccaccggct gaaataatgg ccaaactcga ctggcagtgg
    10021 cggcagcttc ctgatcatgg aatcgtaacg acccggaacc atcagagcgc ccgagaaagc
    10081 ggggcctcca tagaattttg atcccgtaaa gagaacatga aaaccatttt tcaaaaaagc
    10141 gttcaggctt ttacgactga atcgcccctg ggcggcatca acaacaacgg atatgtcgcg
    10201 cggatatttt cgctgcgcct ttcttaccgc ggatattccc ggagcaaaga taccggtctt
    10261 gctgtgtgcg acgatatgaa tgagcacgcg ctcccgtttt gcgagcgctt catggatcaa
    10321 cgcatcggtc tccgcatcga tatcccccgg ctcacgcacg caaccgttgg catcgcgaat
    10381 cggtatcatt tcgacatgga caagcctggc aatagctgaa gaaatgggag tatccttgac
    10441 ctgccgcgga ccgtgaggca ccaccgcgtc aaagtatcga ccggcggcag ccagtgaact
    10501 gccgctgcct atctcctgcg gtccgacgac gatattgcaa agcggacggc ttcgatcacc
    10561 cagcgccagc gccaaaacca gaagctcggc atccgtgccg gacggcgtca acgcgatgcg
    10621 cacatcctgt cggctgattc ttaaatgacg acgaacagcc atcctgatcc ggcaataacg
    10681 atgacggata tgttgcagca attcgccctg cagatgagca tcgcgcagtt gggcatgctg
    10741 ggtaaccgca gcatcgaaaa cccgttgaag aggtgatgat gccgtgcagg agccaagagg
    10801 cacggcattg cgcggtccgg gggtggtgcc gtacggcgtc aggccgtttt caccagtctg
    10861 gcttctgctg tccgctccga tttcgagcaa tgcttcagtc cgcaactcga gactgaccgg
    10921 atcagcggca aaactcttga ttacttttaa cattcaaaat gccttcaata acaaaatgat
    10981 aaaaccttta tgttccggga aaatggaaca ttaccggcag ccgtaatgat caacgtcaaa
    11041 ccacctgtta aagatcgtta tagtttccgc cgggtataac gcttcgtcgt cttttttacc
    11101 tgaatttaat agtcccaggc gcccttatag catctgcaaa atggggcttc atttagaaat
    11161 tataccccta taagtcaaga taaattttta ccataaggca atcctgcttc gggaatgcaa
    11221 agaattccgc gcttgtgcaa tgaatgacaa cataggcatt ttcatggaat gcaatcacgc
    11281 actatgacgg cagaggatat cggctggtga tctgcgcaaa cacttcgccg cttttctctt
    11341 ttaacatgat aaagctgttt tgaatcggca aatcaacata taaaaagcgt cccttatccg
    11401 gcccgatgcc tagagacgcc agatcgttca tatgcgctaa attggttgaa aggatatccg
    11461 tatccatatc ggtcgcattg taatggttga acatatccgg tttgaattcg atcccgtatt
    11521 tcgacacata ggatatgtcc tgaatgaaat tttgatctct cgcgctccgg atggattctt
    11581 tcaggcggta agcaatctca tatatggaca actgatcgat ttcatccttg gtaaactccg
    11641 tccagctgaa gaaaaaggca ttgccgatat acatgggatc aatgtcgggg tgtatctctc
    11701 tcaaattgac gggatgcctt aatcggacct tgtcagtatc cggcaagatg tgacgatgat
    11761 attttttcaa taaacaggac accataatct gattatcgga aatctcatat ttctcatggg
    11821 ttgattttgc cttctgtttg tgttcacgga gaaattcatc cgaaaaatac tctcttttct
    11881 gatagatccg ggcatgactt gattgatagc gattctgtat ttcatcgttt aaatcagaca
    11941 aagggggaaa gaacaatgga tcaatccgtt cggatttaac gggttctcct ttgaataatc
    12001 gttgaggcga cggcagcggg agctcattgc cttcgataat acacccccat aaatgcagga
    12061 aaagaatcaa tgaaaatcca tcagcaatca cgtgcgaaca actgatgccg ccgaattttc
    12121 cgtctttcac ggcaatgccc gtaagacaaa gcagcggccc gccgggcaat gttttaacct
    12181 ttttgatctt cttccggata tcctcaatat cgacgctatc agaggtggca tccatgggcg
    12241 gtaaaaaatt aaactcaacg ccgtccgcgc agtagtgtaa cgcataccga tcctcatcca
    12301 tcctgatcaa gcggctcgaa aagagattga agtaatgcgc cgctttcaga aaagcttctc
    12361 cgaattgttc atacgtccaa tcttttttat aaaacaacaa aacttccaat ggattagatc
    12421 cgatcagtgt tttgttttcg agaagggaca acggcagaat cgttttgcca agattttctt
    12481 ccaaatagct acgttgctct ttcttaagta tcacttaaat atctccaaaa ataaatttat
    12541 tgcaattgtc ttcaaaaacg acaacaaatt ttaagaaata tttttgcaat gattttaaca
    12601 ctttcctaaa ataatgtgac tatttgttgt atgtacctct gcagtcaaaa cagcacaagg
    12661 tcccctacac gtttcgttca gactaatgaa ggcggaatgg atacatgctt gttatctgag
    12721 taaaaatcct gccttttttt tctttataca tcataaaact tgtttgcact gttgaattga
    12781 gatgtaaaat attgaagagt tttgaaagac ctaacgattc aggatcattg agaagcacta
    12841 aattgcttga aacgatatct gtgtttacat caagagaacg atatttttta aatatgtcag
    12901 gtttaaactc aatcccatac tttgataaat acgatatatt tttaacatag ttctcatttg
    12961 tcgtcttcgt gattgattct tttaaacgat aggcaatctc gtatattgac attgtattga
    13021 tttcatgtct ggtaaattcg gttttattat caaaaaacac attaccgata tacatggaat
    13081 caatatccgg atgaacatct cttaaattga caggacttct taatacgatt ctatctgtat
    13141 caggcagaag ttgatcatga tattttttta atagaaatgc agtcataatc tgattgctgg
    13201 agatgatata tttctcattt tccagctttg ctttattttt atgttcatcg aaaaattcat
    13261 ctgaaaaata ttctctcttc gaatataatt ttatttcact tgccctgact cggctttgaa
    13321 tcttttcgct caacgccgac aacggcggag tgaatatctt atcaatggca tcggacgcaa
    13381 caggcttgcc cttaaataat ctctgagttg acggaagggg aaagctcttg tcctctatga
    13441 tacacttcca tgtatacaaa aacaggatgc atgaaaacac atccgctatt gcctccgaac
    13501 agctgattcc acccaataat ccatctttta tcggcattcc tgtaactgca aaaagaggtt
    13561 ctcccggtaa tgttctaaca tgggccatct tttttttcat ctcatcgaca gcaatatgat
    13621 ccagcggagt attcatcgac ggcaagatat tatattcgac gccatcaatg cagtgttgta
    13681 atgcaaattt attgtcatcc atcatgatca atcggcttga aaaaagatta taatactcaa
    13741 tagccctgaa gagactttct tttattttta aaagtgaagt ccttttctta tagaacaata
    13801 caatttccat cggctttgtg cccggtctga tttcattttc aaatattgat aacggcagaa
    13861 tagttttgcc gagattttcc ttcagatata acatttgttt ttcacccagt ttcatcatca
    13921 tgtctccaac agaacatttt acatatttgc tttcgaataa tttgaaggac gactacagat
    13981 tatttctata tcaagatttt aatctcaaga agaataatgc ataaatttat tattatcggc
    14041 tcagtcactt taaaaagaaa gcaaacacct gcgcctactt ctctatcttc tcttttacct
    14101 cctgattatt gttttcttta ttcgtaagtc ttatttaaat atttttcaaa tcattacacc
    14161 atttcattta agagcattac aagcatattg tttaatcaat ttcaacttct ttgaaacctg
    14221 tacagtatgt ttttcttgca agccgtcttg aatattaaat gctgtgatca ttttaccagg
    14281 cagaatcaaa aatacatcaa atatttgata tgatatttac ccgttgctac gatcttcaat
    14341 cttacatgcg aggttatctc caccgaaaag gtaaagggtc gctatcgatt catcctccgc
    14401 ccctataaag aatcgcaaat catttatgat ttttctcgtc ttgctacaag ggctgcttta
    14461 ttttgttatg gaagcatgac ctttttataa tggcatttta caggagaaag ctgatatacc
    14521 aagcagctat gaatcagaaa atgttatgca aaaacgaata attaaaatac ccgcgtggaa
    14581 gactgagttc tgaacctgac ctgtggccag aaagcgctgc tcaggcaggt gattttttta
    14641 tcggtcgcat gccaaagaaa aaacgcacga cgttaaatcg tctcacgaat aaatcataca
    14701 cggccataat cagcacaaag gagattaaag tgatgatcaa aaacttcagc cccatgccga
    14761 tattcaactg tatgacaaac caaccaacgc caagaataac aggttggtgc aacaggtaaa
    14821 aaggaagtac cgcttcgttg ctgtatgtaa gcaccttgtg gttgaaattc agatattttg
    14881 ctcccaggct cagcacaata acgatccagc tccaacggct gacactcatg atggtttcga
    14941 agaggacata ggttaaggag aatgactcac catccggata attgtacccc aagcccagga
    15001 caaaaattcc ctgacctccc caacaggcaa gacctaatat cagaccaaac cagccatatt
    15061 tttttatact ttgcataaag cggctgtctg ccacaaatag gtagcctatg ataaaatata
    15121 ccatatattc aaaaaaatca gcccaggtaa actgtcctaa aaagaaactc ctcaacccga
    15181 cccgcatgaa taaaacggga atgatgaaga taaaaattcc gcctggcctg gtgcaccatc
    15241 cggctaattt atcgataaaa cgcagaccgt gcccggaacg cagatagcac atgaaaggca
    15301 gagaaaccaa cgagaccaaa aacaggtacc cgaggaacca caaatgcgtg ataaatgata
    15361 acattaacaa atcgggcgga ctgccgatag aaaaatgacc ctctcgatcc agatagagtg
    15421 ggatgctttc ccaaaaggta ccggtgaatc cggcattgct gtatatttca aaataaaact
    15481 gtggcggcag caggataaaa aaacctactg tgtataaagg gatgagaatc cgtttgatcc
    15541 gttcctgaac gtactgtccg tttgtgagat aggcaagggc gtaccacgaa ccgataccgg
    15601 aaaggaagaa aaaaaacggc ataacccaca tgtcgatcaa agagacatac accagggcca
    15661 cgatactctc ttcggcattt ttcaaaagcc aggttccacc tccgaaaaac cgggagcagt
    15721 ggaacatgaa tacgagcaac atcacgatga cccgcaacca atctaaatcg tatcttcgtt
    15781 ctttcattat ctttcatccc ttaggcagcg ttcatgcttt ccatcaagaa tgcgctcctg
    15841 tttcatgcca aagattgaaa tcttttatat ttgtcaaaat ggacgatttg tcttaattag
    15901 gaaaagggct tatggagaat aagcctaaaa acaaatattg ttaacgcagt atcatttcat
    15961 atatgattta tgggaaccaa ccattatccc tttaggggaa gtcaagcggt catggtcttt
    16021 ttccattgcc tgcaggcaaa tccattactg accttctcgc tcgagatagc gtcaggtgaa
    16081 atacgccata tcatttactc caatggatat ctacttgtta tctgtgccca tatctgtccg
    16141 cttttttctt tcaacataac aaaacttgtt tgagtgggga tacctatata tacaaaatgt
    16201 cccttatcct ctccaatgcc caaagcccca agatcattca tgtgcgccat attgcttgaa
    16261 acgatatccg tatccatatt cgcaaaggcg ggacgagttt taaacaattc ctctttaaac
    16321 gttatcccat actccgataa atacgatatt tctttaatgt agttttcatt cctcgcattt
    16381 atgactgatt tttttaaaca ataggcaatc tcgtatattg actttttatt gatttcgtcc
    16441 ttggtaaatt cagtgaaatt atcaaaaacg gcattaccga tatacatgga atcaataccg
    16501 ggatatattt cacgcagatc aataggaatc tttattctaa tcctgtctgt atcaggcaga
    16561 acctgatcat gatatttttt taataaaaat gcggtcataa tctgattgct ggagatgata
    16621 tatttctcat tttccagctt tgctttattt ttatgttcat cgaaaaattc atctgaaaaa
    16681 tattctctct tcgaatataa ttttacctcg cttgccttaa ctcggttttg aatcttttca
    16741 ctcaatgccg acaacggcgg ggtaaagatc ttatcaatgg tatcggaagc gactggcttt
    16801 cctttaaata atctttgagg cgatggaagg gggaagctct tgccctctat gatacacccc
    16861 catgtcatta agaaaagaat cagtgaaatt ccgtcaccga ttgtatgcga acagcttact
    16921 ccaccaaatt tcccgtcttt tgccgatatg acagttagag aaagcagagg ttctcccggc
    16981 aatgtcttaa ccttgaccat ttgtttcctt atttcatcaa tattgatatt gtcataagta
    17041 tcatcaaccg gcggtaaaaa agtaaatttc actccgtccg tacagtagtg taatgcaaac
    17101 ttgttgtcat caatcatgat caatcggctt gaaaaaagat tataatgctc cgtaaccttc
    17161 aaaaaagatt cctgaatctc cgagttggac cattctttct tataaaacaa caagacctct
    17221 atgggttcgt tgcccggcca gtcttcattt tcaaagatgg ataacggcag aatcgtgttc
    17281 ccaaggttat tttccaaata ggcgcgctgt tcttcattaa gtttcatttc aacaactcca
    17341 ataaaataat ttgatactga cctttgaata acgtgatggg ttaataaaaa aagttcgagc
    17401 gttaagacgt taatcgcaaa ttgaaaattg gatagctttt atattatcct tatggtgtcc
    17461 attaaatatt acaataaata ttttcttaca atttttttta tatttaaata gtcggtaaaa
    17521 aattaatgat cattggcgtt agtattccat ggataacaaa cggcataagt caatatcttg
    17581 tattttatct acaacaaggt ctgctttact caaatcctgg cttcccgagt tcatattctt
    17641 gaaaccaata catctcattc ccgctttctt tgcggcaatg acgccattct tggaatcctc
    17701 aatcacgacg catcctcgcg ggttcacctg cagtaattcc gccgttttca gaaagatatc
    17761 cggttccggt ttgctttttt ctatattctc accggacacc catgttctga tgtatttttc
    17821 tattttgatt ttatcaatca cttcttcaat aaaccggcct ggtgaagaag acgcaacggc
    17881 aacagggatg ctgcgatcat acaatgattt taataattcc ggtattcctt caatgggatt
    17941 gcaatccgtt tgtcttaata attccaactt cgttgaaacc gacatatcca atatttcccg
    18001 caaactgctt tttatgtcaa attcatcttt tattttctgc cattttaccg gatcggaaca
    18061 accaacatat ttattgtgat atccttcact cgtatcaaca tgaaattttt ttagcagcgc
    18121 tatggctgcc gaaatatgca atggctcgct gtcgatcagt acgccatcca tatcaaaaat
    18181 tacagcttcg tttctcatga attttccgcc tctggaacaa ttaccttttt tatattattg
    18241 aatttataaa atatttgccg acggcgcttt ataggtgact aacccgtggt tgtcaagcag
    18301 agattaacag ctcctgcaat tgattgaaaa tattaacgtt ccgccgttaa gaaaaaaata
    18361 taaacttgtt aagtttagaa atatctagta ctaatccata ttacatagat gatgaacgaa
    18421 aaggatccct cgccaacagc aagatattgc atggagaaga atatgctgca ccacaaatct
    18481 tttacacaga tacataaccg ccccgcagaa atactggaga agctgatccg attcaataca
    18541 accaatccgc cgggcaatga agccgcctgt ataaactata ttcgcaacct gctgaatgat
    18601 gccggaatcc aatccaccat tattgccaaa aaaccggagc ggcctaatct gattgcgcat
    18661 attgacggta ggggaaaggc taatcccatc cttctctacg gtcatgttga tgtggtaggc
    18721 acggataacc agcaatggga tatcccgccg tttgacggta aaattatcga cggcgttgtc
    18781 tggggcagag gcgctctgga tatgaaagga ggcatcgcca tgatgctggc cgcccttctg
    18841 cgtgcaaaag cccaaaactt caaaccggcc ggcaatgttc tactggccat tgtcagcgat
    18901 gaagaagcag gcggcgatct cggaacaaaa tttcttgtgg aggaacatcc ggaactattt
    18961 aaagatatcc gttatgcgat cggcgagttc gggggcttca atgttaatct ggataacaag
    19021 cgcttttatc ccattactat ctcagaaaaa caaatctgct ggatgaaagc gaccattcgg
    19081 gggcagggag gacacgggtc catgccggtt cgcggcggtg cgatgatgaa attatcagat
    19141 tttctcagac gattggaaaa aaatgatttg ccggttcacg tcacggtccc ttcaaaaatg
    19201 atggtggaag ccgtagcgga cgcactggga ggtatcaaag gactggtgtt cagccagctg
    19261 agcaatccgt ggatgaccgg aatcatttta aacctgctcg gcgcaagcgg aaaaggtttt
    19321 taccctttgt ttcataacac ggtcagcccc accattttgc atggaagcgc aagcatcaac
    19381 gtcattccca gcgaaatctc cgttgatttg gacggacgcc tgctgcccgg ctataaaccg
    19441 gaagacatga tgaatgaatt gcgaccgctt gtgggcgata acgtccggtt tgagctgatt
    19501 aaatatgacg ccgggccggc aaaaccggat ttgggattat tcggcgtttt gtcacagatt
    19561 ttacacgaag ccgatcccga aggaatcacc gtcccgttca tgctaagcgg cgtcaccgac
    19621 ggacgctttt tatcgaaact cggcattcag acatacggtt ttcttcccat gttgttgcct
    19681 gaagattttg atttttcagc gaccattcat gccgccaatg aacgggtgcc cgtccgggcg
    19741 attgaatttg gcgcggatgc gatgttcaaa gttctggaaa gatttggaca agacgaataa
    19801 ttctttttcg gcaaacaaag aggctgcccg gctatttcct gagcgccagc aagcggtctc
    19861 taatcgccct ccacgtattc ttgaagaaga ccttgggaaa ctggataacc atcgccggca
    19921 acggacggca gtatatcagt attaaaagcg cgtttccttt ttcgaggccc agattttctt
    19981 taaaatattt attcacgtgc gcctcgggac aaagaggcat actggcatga gttattgtgt
    20041 tttctttttc atcggtaaga atatgccgga aaacaaataa gttttcgttt attttatcct
    20101 ccgtatatcc caacttccgg agataatttt ttctaatgtt tatttccttt tccgagacat
    20161 gagacacatt gatgtctcca tttttgtcta tcggcgggga aatgttcatg ggcgacgcga
    20221 agacagcgtg gtagataatc ggattaatgg ccctgccaaa catatacgcc gtcctgaaga
    20281 acggaatgat acgctttatt cccgtatatc ccatttcccg attgtatttt ttcagataat
    20341 caaggaacat cttgggaatc aacgctctta ataagccatt tttcctgtat tcttccaggc
    20401 acatggtcaa ccatatgatc atgacaaaat ctccctggtc gaacatggaa ccgcataccg
    20461 acatggcgac aaccttatgg ttcatatcgc tgtagatgta cattaagtcg gactcgctaa
    20521 aatactttgg aatattaatg gcgacttcct cgcgcagcgt tttacgggcg acttgcctga
    20581 tattctcgaa taatgattct ttgtccacaa aatcggaatc tttcaaatca cagggccggt
    20641 tcacttcttc aaagatgaaa tttgttcctc tgaatttttt tactctgact ttggatcctt
    20701 taatgtgaaa aggcactttc tgattttttt tattcatgag tcttctcttt tttagaaaat
    20761 gaacggtcct gaattttgca tcacgtttca tgcaacataa cgacaatcca tagcggacat
    20821 tataatcact ggtgggcgat gtgagattcg aactcacgac ccctggcttc ggaggccagt
    20881 actctatcca actgagccaa tcgcccacgc tcggagcttc tactacatca cagcataatc
    20941 ttcaatgaat aaaattcatc acaaatgaaa ggggtatggg gatagaaaca agacgccttt
    21001 tcatttctcg gcatcatttt ggtgtgacct tgccgaatgg tggtctttcc ccagataaaa
    21061 attgatccac gacgacgttt gataaagatc ccagttacag ggaggaacga ttttgtgctc
    21121 caggtgttct gcttcgccat atttttttaa tcggacatac gcccggacat acacacaatg
    21181 ttttttaccg ggatacactt cgcaccatcc tttaaagctt ccgccgcagg gaccgttgcg
    21241 ctgatttttc ggacattgag acatcgggca aacataagcg acatcaagga gagcgcaatc
    21301 cccgcaatcc ttgcagtcgt aaataacgac cttcgcaaca tgttccagat tgtggaatat
    21361 ctttgccatg ccggtgcctg atattttttc actgactgtc ttcatcagcc cgtaaagttt
    21421 cttgcccggt tcaaacatca gcttatgaaa aaaacgggaa aacccgtatg tccattcgac
    21481 gttggaatca agcggacgat tctgtcttcg cacaggcgtt tctttgttca ggcccgtaac
    21541 cggatcgcgt tcataataat aaaaaccatc agagagggga taatcaaaat atccgacaag
    21601 atcctgccac tgcgggacga gcgcttctcc ctggttgata atattttcca cctgctcata
    21661 tttgatattg tgtccgccaa tatgcactcc gctgtatccc atacctttca gtatggcata
    21721 cagccgggca gcacgcaaaa ttctggcacc ttctccctta tcggaatcgt ttctttcgcg
    21781 gtcaatctcc gcaagcagct tgtccgtaac gacggatccc ggcaattcgt tgcggttcat
    21841 cattcgggcc gcgccatacg ggagtatgta aatattgccg acaagaggga tattgaagcc
    21901 gtgttgcctc ataaacaaca gcacctcatg aaacttgcgg gcatcatagc cgagctgtgt
    21961 aataatgaac tgagcgccgg cggcgatttt ctttttaagc ttgaaatact gcgtcatctg
    22021 ttccgcttcg gtcgccttga acggagaaat gaccgcgccg gggaaaaagt cgcttgcctg
    22081 atgacgaaca acccctttga ttcccggata ctctaatccg gcgttcatct ccgatataag
    22141 ctgcagaacg tgaatcggat ccagatcaaa taccggagcc ggtcttccat gaaaaccgga
    22201 gacagggtaa tccccgctca tcaccagaag gtttcttacg cctgaccggt ccagagcata
    22261 gagctgactt tcaatctgat tgcggttctt gtccttgcag gtaaaatgaa cgagcggctc
    22321 gataccgaac ttgacgattt cagcgcccat aaaatcggcg gacatggcgg gcgttccgcc
    22381 gggattgtcc gtcaatgtca gcgcgcagat tttgccgccg atagacgcct gtttcgccag
    22441 ggccagcgcc ttttcctgag ccgtttctct cgcccccctt cccggcacga gctcccaggt
    22501 aacaggaaac acggatggat tcagcagggc gtctttgaag agattttccc gcataaatgc
    22561 tttcaacgtt cgtctaaatt ttatgcggtg aaaatgacat tataataaga gcttgtcaat
    22621 atcttttcta ccaaacagtt gcagttcgcc gtcaaatttg ctatttttta attatgttgt
    22681 gcaaaagaac ttttatcctg aaagcatttt accggttttt ggtaatg
//
LOCUS       DAOOLB010000031        21994 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_2303_length_21994_cov_44.444505, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000031 DAOOLB010000000
VERSION     DAOOLB010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 21994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21994
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2303_length_21994_cov_44.444505"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="PLF77_05610"
     CDS             complement(<1..421)
                     /locus_tag="PLF77_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPC08367.1"
                     /translation="MAGAELIGKEEIKEVMDVLETGILMRYGFDKERKGIYKVREFEQ
                     AFAKYCGTDYALGVTSGSSALQVALTAMDVGPGDDVLVPAFTFLATYEAVMEVGAIPI
                     MVDIDDTLNFDPLEIEKKKTPYTKAVIPVHMCGSAANI"
     gene            complement(439..1596)
                     /locus_tag="PLF77_05615"
     CDS             complement(439..1596)
                     /locus_tag="PLF77_05615"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPC08368.1"
                     /translation="MRKTFSFTGAKKIVFGSGSLLTLASHVKEHHARNPLVVIDKNLA
                     KTDLLEKVANVLIPSGIKFTVYEKVEAEPPIELADEGAKAAMKNKCDIVIGIGGGSAM
                     DVAKAIAVIATNKGAAADYLGLNKVPKPGLPKIMIPTTAGTGSEVTFTAVFLRKNLKK
                     KEGMNSPYLYPELALLDPELTLSLPPVPTAHTGLDALCHAIESYTSINSSPVSEMFSL
                     EAIALIAENLRTCVHDGKNIVARERMLLGSLYAGIGLANAGVTAVHSLSYPLGGKYGV
                     GHGLANTMLLPAVMAFNMPSALEKFTDIAEAMGENVEGLPEREAAYLALEAVEALIED
                     CGIHDTISTFGVKEKDFPALADVAVTVARPLENNPRKLTKDDMIAIYTQAF"
     gene            complement(1732..2472)
                     /locus_tag="PLF77_05620"
     CDS             complement(1732..2472)
                     /locus_tag="PLF77_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPC08369.1"
                     /translation="MLKTQLKNISVVLYKPKYAGNIGAVARAAKNMGIRKMIVVGTDD
                     FDREEMRQRSTHLAADVLDDIQYIENAAEAVSPFNYIVGTTARTGKARGPFVTPRQAA
                     QTIADISQKNKVALLFGPEDTGLANEQLRLCHMVVTIPTSREFTSLNLSHAVMILCYE
                     IFTASCIAVTASEATPKLALSSELEGMYGQIKTLLADIGFLNPENPDYWMMHLRRFFT
                     RAGILSREVKIVRGICRQLQWYAHHKKT"
     gene            2744..3661
                     /locus_tag="PLF77_05625"
     CDS             2744..3661
                     /locus_tag="PLF77_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family murein peptide amidase A"
                     /protein_id="HPC08370.1"
                     /translation="MVKVKISLISVGLVLLFAFFLLLPLQSFAYDCSTREGKQYLRLL
                     ERKFADYRWTDLHPRDISWEYHRTTRNKHPLMFVHFGNSSKNCVLFFGGVHGDELPTA
                     YLMFKLAHYVKEHPALFKDQCIVIAPLLNPDGFLSAQPTRTNASGIDINRNFPTRDWK
                     AKAIRQWIAKGKQVRYYPGTRPASEQETIFQMALIKRFKPQKILSAHSPLNFFDYDGP
                     SSDLDSFEKWMEQICKESNHPLKKFGYYPGSLGNYAGHERNIFTLTLELPSSDPGQSS
                     DYFEKFQPSIVKFIQLPIVGSPPNLKVLR"
     gene            complement(3762..4127)
                     /locus_tag="PLF77_05630"
     CDS             complement(3762..4127)
                     /locus_tag="PLF77_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08371.1"
                     /translation="MIKKNLGQYLAQKKFIRKILDDPVDLSEFKQRPTPRLIGGLVLM
                     ALSYVIGWPIIFVLSILAVSFHEPLIFVIGGPLAYAVSCVVFLIGAWMARAPHYMNTL
                     TRYALQSLLKKLFDPVRTD"
     gene            4298..5014
                     /locus_tag="PLF77_05635"
     CDS             4298..5014
                     /locus_tag="PLF77_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08372.1"
                     /translation="MNSSTNGWDFFDRFYCISLRKREDRRQSARKEFEKVGLANRVEF
                     VIGERLSDDFEREVYEAHMLCLRKGLEAGAKNIVVFEDDVEFDRFDPERLQKCTDFLR
                     RHPGWKVLLLGALIRSSRNTDDPCVQKVRYQSLTHAYALNAPYAKTLAYDPWSGIVND
                     TLFRPLTEDVYAVCPMCAFQKNFTSDNYKYPRLALIRRLFGGLKRIQKANEFYQRYKV
                     GVIAAHLAVVILILFLLWRG"
     gene            5425..5598
                     /locus_tag="PLF77_05640"
     CDS             5425..5598
                     /locus_tag="PLF77_05640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08373.1"
                     /translation="MSKLNLTQEEENELLLILERYLPDIQMEIANTDSKEFRRELKER
                     EVFMNDLIARLKR"
     gene            complement(5737..6411)
                     /locus_tag="PLF77_05645"
     CDS             complement(5737..6411)
                     /locus_tag="PLF77_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPC08374.1"
                     /translation="MKKIIICFFLCGTLMLTGCAQTGTMTKSQTGGLVGATGGAVAGA
                     VLGQVIGRDTKATLIGAAIGAAIGGAGGYGVGTMMDNQERDMRDALATSEAAAVTREG
                     NLLSVTFKGDVTFDTNSAEVRPGLYSEINRVAAILNQYPQTLIQIEGHTDSVGSDDYN
                     MKLSQRRANTVKTLLVQRGVAASRIETIGYGETMPVATNNTEVGRQMNRRVEIKIAPQ
                     TQAQTP"
     gene            6522..7259
                     /gene="rsmG"
                     /locus_tag="PLF77_05650"
     CDS             6522..7259
                     /gene="rsmG"
                     /locus_tag="PLF77_05650"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HPC08375.1"
                     /translation="MTRWTPMTGHEYNLKFAAVDLFCYCTGMNHDIAFLKNQARQLNI
                     ALSNGQLQQFDVYKNELMDWNAKTNLISEKSARDILARHFLDSLTALRYITPPDARII
                     DVGSGAGFPGLPLKIAQPSLDLYLLEANRKKVSFLKHIVRLLNLSSVDIIHDRVENII
                     RENVYQEKFDVLISRASFKLPQLCSFCRYFLTPAGRLIALKGPGVEAEIQKCFEDRNQ
                     SGIYQLIQQPIEAPFLNSTRKIIIGQR"
     gene            7344..8111
                     /locus_tag="PLF77_05655"
     CDS             7344..8111
                     /locus_tag="PLF77_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC08376.1"
                     /translation="MNKIICIANQKGGVGKTTTAINLSASLAAAEKKTLLIDGDSQGN
                     STSGVGVDRAVLETANLYHAMIGDVPLKDVIMHTAIPHLDVVPSTQDLIGIEVEFVGL
                     EDKEKRLKQLLNDLDARYDFIVIDCPPSLGVMTVNALVAADYLIVPLQCEYYAMEGLG
                     YLLNTVKLVKAQLNPHLVLGGILLTMFDQRNLLSHQVSDDVRKHFGDKVFKTVIPRNV
                     RLSESPSHGLPVILYDIKSRGAMAYMEMAKEIIQGGL"
     gene            8112..8969
                     /locus_tag="PLF77_05660"
     CDS             8112..8969
                     /locus_tag="PLF77_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPC08377.1"
                     /translation="MAQKNVLGKGLGAIFPDLINNESDKKKSLYCGIEELHPNRYQPR
                     KTFHDDKQKELIASIKSSGIIQPIIVRKTNSGYEIIAGERRWRAAQAAGLKDVPIVIR
                     EASDREAAELSLIENIQREELNPLEEAGAYVTLMDQFKLSQEDISARVGKDRSTISNT
                     IRLLKLPAKIQSALMANQISAGHARCLLACPSPEEQIAALTLILKKDLNVRETERLIQ
                     RAKKIPLDKKQPVKKDRFLNDLEKAMTVKLQAKVQINGSDKKGLIEIRYTSMDELNRL
                     SGLILDDLQ"
     gene            9004..10377
                     /gene="dnaA"
                     /locus_tag="PLF77_05665"
     CDS             9004..10377
                     /gene="dnaA"
                     /locus_tag="PLF77_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPC08378.1"
                     /translation="MFVDKGFNMDAFWDKTSKIIQEKISKQNFDTWIKPIQIVAMEEQ
                     CVKLAVPNKFFRDWLVDNYSSTIKQSLKDASGLDVNIDFILSKTKETDHEPKAKQTAA
                     AKTAAPAVHSRSKTFSFLNDNYSFERFVVGSCNQFAHAASVAVAKQPAKNYNPLFIYG
                     GSGLGKTHLLNAIGLMANSIHPSLNIMYVSAEEFMNEMINSIRYDRMPKFREKYRNIS
                     CLLIDDIHFLAGKDRTQEEFFHTFNTLHDSGKQIVVTSDKFPKDIPNLEHRLRSRFEW
                     GLIADIQPPEIETKIAIIERKIQENKIELPNNVIHYIASHVESNIRELEGFLVRIGAY
                     SSLTGREIDLDLVKEVLSNLVKHNHKGEISIEEIMKVISGKLNIKISDIKAHNKNKNI
                     VFARQIVMYLARKLTNQSFPDIGQKIGGRDHSTVIYANNKIANNIKSDIKLKNLIQEI
                     EDILIHK"
     gene            10529..11638
                     /gene="dnaN"
                     /locus_tag="PLF77_05670"
     CDS             10529..11638
                     /gene="dnaN"
                     /locus_tag="PLF77_05670"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPC08379.1"
                     /translation="MEFKINREIFLDGIQKTLGIVEKKTTIPILNNILIKAEAGKIKI
                     IATDREIILISDYEAEVMEKGEITISAKKIYEMIREIQGEFINFIQKNNLVKISAQRA
                     FYKIPGLPADDFPSIADDQDIPFSKIQGSIIKDLINKTSFAMATDETRKNLNGVLMEA
                     GLDGSNYLLRMVATDGHRLAMARGFTPEPTLKTVKGIIIPRKGLMEIKKIIDENKEVQ
                     IGLHKNMFLVKTENTLLKVSLVDADYPDYKKVIPTEKGMSVVLEKDYFLHALRRMSVV
                     SSERYGGVILSFSKGKLTLNSTNLDVGEATEEIDIDYGGEAIDSGFNVNYLIDAIAVV
                     NKENIVFEVGLGLKPSIIKQAEDDNYLCIIMPLKI"
     gene            11666..14071
                     /gene="gyrB"
                     /locus_tag="PLF77_05675"
     CDS             11666..14071
                     /gene="gyrB"
                     /locus_tag="PLF77_05675"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPC08380.1"
                     /translation="MTHNYDADSIKVLEGLEAVRKRPAMYIGSTGKEGLHHLVYEVVD
                     NSIDEAVGGFCDTITVKIRVDNSIVVDDNGRGIPIDMHKTEGVSAAEVVMTKLHAGGK
                     FSNDSYKISGGLHGVGVSVVNALSKTCELEVRRDGNVYMQSYKRGIPDAPLEKIGKTR
                     GKGTKVTFLPDDEIFEETEFSYDIIANRLRELAFLNSGVKIILIDERANKQNEFFYKG
                     GIISFVEYINRNKKVLHKNPIYVSGAREDCSVEVALQYNETYLENIFSFANSINTTEG
                     GTHMIGFRSALTRVFNNYATNNKILKDGKESLRGEDLREGLACVISVKVKNPQFEGQT
                     KTKLGNSEVRGLVEGIIYDKIGSYLEENPSVAKQILNKCMEAARAREAARRARELTRR
                     KSALEVGALPGKLADCQEKDPALSEIYLVEGDSAGGSAKQGRDRKNQAILPLRGKVLN
                     VEKARFDKMLQNEEIKTMVTAFGAGIGADDYDISKLRYHKVIIMTDADVDGAHIRTLL
                     LTFFFRQMRELIEKGYLFIAQPPLFKVVEKKQEFYLQNEEQMKNYILENGVGKVKLMM
                     GDGSPFPVNGNKLLTLIKKVMRIKDQMERFEKEGRDGNLIRILAGDPTLTDDSFKSEK
                     ALAAVAKRTGMALGESFADFQIETDEDHGGFKIVFNLKKNGHTVSSCINREVFRTPQF
                     EDIRLLLDQVAIIGEGPYSDKGDESSTPVVLENPKALVDYIVSVGKKGLTVQRYKGLG
                     EMNPEQLWETTMNPEKRTLLQVTVEDAVEADTIFTTLMGDQVEPRRDFIYANAMNVSN
                     LDI"
     gene            14154..16655
                     /gene="gyrA"
                     /locus_tag="PLF77_05680"
     CDS             14154..16655
                     /gene="gyrA"
                     /locus_tag="PLF77_05680"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPC08381.1"
                     /translation="MERDIHHKQTLVNIEDEMKKSYMDYSMSVIIGRAIPDVRDGLKP
                     VHRRILYSMYETGYRHNKPYKKCARIVGDIMGKYHPHGDAAIYDTLVRMAQDFSLRYM
                     LVDGQGNFGSIDGDPPAAMRYTESRLARISDEVMADLEKETIDFVPNYDESLTEPSVL
                     ATRIPLLLLNGTSGIAVGMATNIPPHNIVEIVNGTIACIKNPAITIEQLMRHIPGPDF
                     PTAGFINGREGILSAYKTGRGIIKIRARALIEKNGRTDKESIVITELPYQVNKAMLIE
                     KIAELVRDKKISGIADLRDESDREGIRVVIDLKRDENSAIILNQLYKFTQMEVSLGII
                     MLAISDGRPQVFNLKEVLEKFIAFRQEVVIRRTQFDLARAKERAHILEGLIIALNNID
                     AIIKVIKASKTPAEASVQLCAKFKLSEIQAKAILEMRLQRLTGLERDKIDAEYAEVTK
                     LIAYLQGILDDPQKVLQVIIDELNEIKQKYGDERRTEIVASSEDINIEDMIVEEDVVV
                     TISHAGYIKRNAVSLYKSQHRGGRGKVGMGTKEEDFVEKIFIASTHNYLLIFTSAGKV
                     YWLKVYQIPQAGRAAKGKAIVNLVNLAPGEKVMATLPVREFVDDKFIVMVTKKGTIKK
                     TELTAYANPRAGGIIAISIDEGDELVEVQLTMGNQDVFIATKQGMAIRFKEDDVRDMG
                     RTARGVRGISLDEGDEVIGMDIPAQNTFMLTVSENGFGKCTPVDEYRVQSRGGKGTIN
                     LKTVEKVGNVSGVLQVIGEDNVMLISNAGKVIRLKVQEIPVNHRVTQGVKLIDLEPEE
                     KLVGVARTTSEAESRDDGNGDDEGDANGEDNGAEE"
     gene            17084..17593
                     /locus_tag="PLF77_05685"
     CDS             17084..17593
                     /locus_tag="PLF77_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08382.1"
                     /translation="MELIFQGSPKIVVATNTFINVPVILKYEDTPLLEVIKEQGIGFM
                     TQIPIYHSDGTYLAKVTGNRVYPTEEGKKAKIEIRDNPGKFVCSLDKRVLFELSHGVG
                     DAFKATAELYTPDGCFVKCTDNPKPELINLAGNTMRVGGVTMIGNVFQNLSIGIWLRK
                     DGSCAIGVG"
     gene            17687..18571
                     /locus_tag="PLF77_05690"
     CDS             17687..18571
                     /locus_tag="PLF77_05690"
                     /inference="COORDINATES: protein motif:HMM:NF037417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HPC08383.1"
                     /translation="MNFELEPYHRDITKEQVVNDITAVARQLGQSTITTDDYRRHGKY
                     SPDVARRRCGSWLKALVEAGLSTQRHNTKAAPEQFIPDLKRVATLLGKPSVTTEEYRD
                     HGRFAPSTVSNHFGTWFAALDAAGLNRTRTLHVTDEEYFENLEQMWVHLGRQPHYTDV
                     QKPFSRYSAGAYEKRFGSWRKALEAFVVFVNQESDKTRQSRPPASKKKKEYTMPAVQQ
                     KPSRYISWRLRFLVMRRDDFKCRICGRSPALQPGLVLHVDHVKAWIKDGETVMDNLQT
                     LCEQCNVGKSDLSMKKEG"
     gene            18668..19438
                     /locus_tag="PLF77_05695"
     CDS             18668..19438
                     /locus_tag="PLF77_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08384.1"
                     /translation="MDDKSKNPLEMIKDPYAGDSFILPDTLCTDQRRSFFRRSKEILK
                     FLTISDLALKECKNRYEQLLSQKNLQQDTPLKFGFSDGRSTIMPVSSFLNGCNEGVEL
                     LCRQVFIMIYGAWETYLFEILEQSFPKIGISENILEHSLKIMMKGNWDGKFCKMQNNL
                     GIAYKSGDLVNHFNGFELSYGGTIFENPLLFLDELAKVRHRIIHASSMLDNGKSIFIN
                     AQIFPAYLAFFTLLTEYVDKLFSEKFQYRQVKINPAET"
     gene            19611..20033
                     /locus_tag="PLF77_05700"
     CDS             19611..20033
                     /locus_tag="PLF77_05700"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HPC08385.1"
                     /translation="MSNYKYFDHTADIGVEVTGRTRKELFVNAAEALFDVMIEGKFVR
                     KAAKRQKKITVEGADVADLLINFLRELLYLFNGEQFITGNCEIIEFSNKKIQARLTGG
                     SFNNKKHLIKTEIKAVTYSGAKVEKIESGWKARVIFDV"
     gene            20169..21611
                     /locus_tag="PLF77_05705"
     CDS             20169..21611
                     /locus_tag="PLF77_05705"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HPC08386.1"
                     /translation="MAEIKLEKLDDNRWLLPQAGGMRVPGIIYANEKIYQLLKGDESA
                     KQVANVAHLPGIVNFSLAMPDVHWGYGFPIGGVAAFALDGGIISPGGVGYDINCGCRL
                     MTTNLTLGDIKDRLSDLTTALYQRIPSGVGSTGSVKLSGKDQKKVLEEGSACAIKAGF
                     GLNEDLETTEDGGAISGADPELVSAHALERGKQQLGTLGSGNHFLEIEVVQEIFDEEA
                     AKVFGLHKGQIAVMIHTGSRGLGYQICDDYLALMVKHASETGIALPDRQLACAYLDSG
                     RGKNYLAAMACGANYAWANRQILMHKTREVFEKTLRMSPREVNMRLVYDVCHNIAKIE
                     SFNIGGVLKKLCVHRKGATRAFPAGHEAVPERYRKVGQPVLIPGDMGTGSYVLVGQEK
                     AMAETFGSACHGAGRVMSRSQAIKSSRGRSIARELSEKGVLVMAGSKGTLSEEMPEAY
                     KNVQDVVQVMHEAGIAKKVARLKSISCIKG"
BASE COUNT         6206 a         4998 c         5275 g         5515 t
ORIGIN      
        1 caatgtttgc cgccgaaccg cacatgtgca cggggatgac cgccttggtg taaggggttt
       61 tcttcttttc gatttccagg ggatcaaaat taagggtatc gtcgatatcc accatgatgg
      121 gaatggcgcc cacttccatg accgcttcat atgtcgctaa aaatgtgaaa gccggaacga
      181 gaacatcgtc gcctggaccg acatccatcg ccgtcagcgc tacctggagc gctgaggaac
      241 cggaggttac gcccagtgcg taatcggtac cgcaatattt ggcaaacgcc tgttcaaact
      301 ctctgacctt ataaatgcct tttctttctt tatcaaaacc ataacgcatc agaatgcccg
      361 tttcgagaac atccataact tctttgattt cttcttttcc tattaattct gcacctgcca
      421 tatcgttaac cttcctttct aaaatgcctg cgtgtaaatt gcgatcatat cgtccttggt
      481 cagtttacgg ggattattct ccagcggtct ggctaccgtt actgccacgt cggccagagc
      541 cggaaaatcc ttttctttca caccgaatgt tgaaatggtg tcgtggatgc cgcaatcttc
      601 aattaatgct tccacagcct ccagcgccag ataggcggcc tcgcgctcag gcaatccttc
      661 aacattttcc cccatggcct ccgcaatatc cgtaaacttt tccagagccg agggcatgtt
      721 aaacgccatg accgccggca gcagcatggt attggccagt ccgtggccga caccgtattt
      781 tcctcccagc ggataggaaa gcgaatgcac cgccgtaacg cccgcattgg ccaaaccgat
      841 accggcatat aaactgccca aaagcattct ttcgcgcgcg acgatatttt tgccgtcgtg
      901 cacacacgtg cgaagatttt cggcaatcaa cgcgatcgct tccagggaaa acatctcgct
      961 caccggcgaa gaattaatgg aggtatagga ttcaatagcg tggcacaggg catccaggcc
     1021 ggtatgcgcc gtcggaacag gcggaagact gagcgtcagt tccggatcca aaagtgccag
     1081 ctccggatac agataggggc tgttcatgcc ttccttcttt ttcaaattct tgcgaagaaa
     1141 caccgcggta aaggtcactt cggatccggt tccggcagtt gtcggaatca tgattttggg
     1201 aagtcccggt ttgggaactt tattgagacc gagataatcg gcggctgcgc ccttgtttgt
     1261 cgcgatgacg gcaatggctt tggcgacatc catcgcactg ccgccgccga tgccgatgac
     1321 gatatcgcat ttgtttttca tggcggcttt tgcaccctca tccgccagtt caatgggcgg
     1381 ctccgcctcc accttttcat agactgtaaa tttaatgccg gacggaatca gaacgttggc
     1441 gactttttcc agcaaatccg ttttggccag gttcttatca atcaccacca gcgggttccg
     1501 ggcatgatgc tccttgacat ggctcgccag ggttaataat gatccgctgc cgaaaacaat
     1561 ttttttggcg ccggtaaatg aaaatgtttt tctcataatg tcaaacctcc atcggtatcc
     1621 attgtattgt gacgttgttt ttcatcagat atgcatcttt gatacatgca atcaaacgat
     1681 tagaaaagca atgtttatgg ccgcattcac tatgggaact cccctgcaaa gtcaagtttt
     1741 tttgtggtga gcataccact gcagttgtcg gcagataccg cggacaatct tgacctcccg
     1801 cgacaaaatt ccggcacggg taaagaagcg gcgcaaatgc atcatccagt agtccgggtt
     1861 ttccggattc aaaaatccga tatcggccag caatgttttg atctgaccgt acatgccttc
     1921 cagctccgag gaaagcgcca gcttcggcgt tgcttcagac gccgtcaccg caatacagga
     1981 cgcagtaaat atttcataac agagaatcat caccgcgtgg gaaagattga gagaggtaaa
     2041 ttcccgcgat gtcggaatcg tgaccaccat gtgacagaga cgcaattgct cattggccag
     2101 gccggtatct tccggaccga aaagcagggc taccttgttc ttctgcgaaa tatccgctat
     2161 cgtctgtgca gcctggcgcg gcgtcacaaa cggaccgcgg gctttgcccg tacgtgcggt
     2221 cgtccccaca atataattga acggactcac cgcttcggcg gcattttcaa tgtattgaat
     2281 atcatctaaa acatcggcgg ccagatgcgt ggaccgctgt cgcatttctt ccctgtcaaa
     2341 atcatcggtc cccactacaa tcatcttacg aatgcccatg ttttttgcgg cacgggctac
     2401 tgcgccgata ttccccgcat atttcggttt gtataaaaca acactgatat tttttaattg
     2461 cgttttcaac atggttttgt ttcctgtttc gaaagtattc taaaaaatcg ttattccgtt
     2521 ttgtctgtat aaatcagccc tcaacgattt gcacggagaa ttttcgcctg tcccggccga
     2581 cacccggacg ggtgtataga tattgacaaa cgatttcatt tatcccatga tgcgactcga
     2641 ctctctggaa tcgttcacct ccatcatccg ccctaggacg gtgtggttat gagcttaaat
     2701 ttttgtttta cgcttgcctg aaaagattgg aacgctgacc cgtatggtaa aagtgaaaat
     2761 atcattaatc tctgttggtc tggtcttgct gtttgctttt ttcttattgc tgccgctgca
     2821 aagttttgcc tatgattgtt ccaccagaga gggcaaacaa tatctgcggt tgctggaacg
     2881 taaattcgcc gattaccggt ggacggatct tcatccgcgg gacatttcct gggaatatca
     2941 cagaacaacc cgaaacaaac atccgctcat gtttgtccac ttcggcaata gttccaaaaa
     3001 ctgcgttttg tttttcggcg gcgtgcacgg agacgaatta ccaacggctt acctgatgtt
     3061 taaactcgcc cattatgtaa aagagcatcc cgccctgttt aaggatcaat gtattgtgat
     3121 tgcaccgcta ttgaatcccg acggattctt atccgctcaa ccaacccgta ccaacgccag
     3181 cggcattgac attaaccgga atttcccaac cagagactgg aaagccaaag ccatccgcca
     3241 atggatagcc aagggaaaac aggttcgcta ctaccccgga accagaccgg cttcggaaca
     3301 ggaaacgatt tttcagatgg ctttaatcaa acgattcaaa ccgcagaaaa tactgtctgc
     3361 gcattcccct cttaattttt tcgattacga cggtccctca tcggacttgg attcctttga
     3421 aaaatggatg gagcagatct gcaaagaaag taatcacccg ttgaaaaaat tcggttatta
     3481 tcctgggtcg ctcggcaatt acgcaggaca tgaacgtaat atttttacgc ttacgctgga
     3541 actgccctcc tccgatccgg gacaaagcag cgactacttc gaaaaatttc aaccgtctat
     3601 tgtaaaattt atccagctgc ccattgtcgg atcgccgcct aatcttaagg tgctccgtta
     3661 atatccgccc gccttgcctg acccgttctg cagcgtttcc acttggctgt ccgccggggc
     3721 ttctgaaacg cgcccgctca cacaagcctg gcttgcagtc ctcaatcggt tctgacggga
     3781 tcaaataatt ttttgagcag ggactgcagg gcataacggg tcagtgtatt catgtaatga
     3841 ggcgcccggg ccatccatgc gccgatcaga aagacaacac aggagacggc ataagccagc
     3901 ggcccgccga tcacaaaaat cagcggttcg tgaaacgaaa cggcaagaat acttaagaca
     3961 aatatgatgg gccagccgat tacgtaactc aacgccatga ggactaaacc gccgatcaaa
     4021 cggggtgtcg gacgttgctt aaactcgctt aaatcgacgg ggtcatccag gattttccgg
     4081 atgaattttt tttgggcaag atattgcccc aggttctttt tgatcataac acatcatctc
     4141 cggtcacagg cgatccgatg atcccgcacg gtgtgaacca gacatacatg agacattttt
     4201 tttctgcaac ggcttttctg agacgaaccc ctccttgaca tccctctgcc ggataagata
     4261 aaaatcgtaa agaaaattaa catgacacga tgataaaatg aactcttcaa ccaacggctg
     4321 ggattttttc gatcgcttct attgtatctc gcttcgcaaa cgggaagatc gccgccagtc
     4381 ggcccggaag gaatttgaaa aagtcggcct ggccaatcgc gttgaattcg tcattggaga
     4441 acgtctttcc gatgatttcg aacgcgaagt atacgaggcg cacatgctgt gtctacgcaa
     4501 gggccttgag gcgggagcaa aaaatattgt cgtttttgaa gacgatgtcg aatttgatcg
     4561 ctttgacccg gaacgcctgc aaaaatgcac tgatttttta cgacgacatc ccggttggaa
     4621 agttctgctg ttgggagcat taatccgttc cagccgcaac acagacgacc cttgtgttca
     4681 aaaagtccgc taccagagtc tgacccatgc ctacgctcta aacgctccct atgcaaaaac
     4741 cctggcctac gacccgtgga gcggcattgt caacgatacg cttttccggc cgttgacgga
     4801 agatgtctac gctgtctgtc ccatgtgtgc ttttcaaaaa aattttacct ctgacaatta
     4861 caaatatccc cgactcgccc tgatccgtcg tttattcggc gggctgaaac gtattcaaaa
     4921 agcaaatgaa ttttatcagc ggtataaagt cggcgtaatc gccgcacatc ttgccgttgt
     4981 gatattgatt cttttcctcc tgtggcgggg ctgattttcc atcatgacca taccgtctgc
     5041 ggcaacgcgt caagcccgtg gccgtaaagg atcatgatgt tgcatcttga cgaaaaagca
     5101 cctattgata tacaataata aagcgttaag ttaaaatccc tttcggtttc agacaacatt
     5161 ccaccgcctt gctgacgtgc tgatgcaaaa aaacgcgcgg gtgagcaaac caacgtcaac
     5221 gactttaagt tttttttaaa cctggcagga ggcagcacgg gatgacacaa tcctgtttgc
     5281 ctgcatattc atccatcaaa acactgtcgg acatcaatgg agtatgaaac gggataaaaa
     5341 acgggtattt tgtaaatttc gcatatctgt aaagagttaa cttaccggta acattgcttt
     5401 tatcaaaaga aaggaggaac gatcatgagc aaacttaact tgacgcaaga ggaagaaaac
     5461 gaacttttat taattcttga gcgatattta ccggatattc aaatggaaat tgccaataca
     5521 gacagcaagg aatttcgccg ggaactgaaa gagcgtgagg tttttatgaa tgacctgatt
     5581 gcgcgtttga aacggtaaag gggtttttgt gaaaccccta tggattattc tttttcacat
     5641 catccagtct tagaaaattt ttccgttatc aaaacaaacc gccgggcacg catgacacgg
     5701 gcgtcccggc ggttttcgta tcgatgaaaa atttttttac ggtgtttgcg cctgcgtttg
     5761 aggagcaatt ttgatttcca ctctccggtt catttgtctg ccgacctcgg tattgtttgt
     5821 ggcaacaggc atggtttccc catagccgat cgtttcaata cgactggcgg caacaccccg
     5881 ctgaaccagc aaggttttga cggtattggc gcgtcgctgg gacaatttca tgttatagtc
     5941 atcggatccg acactgtcgg tatgcccttc gatctgaatc aatgtctgag ggtattgatt
     6001 taaaatggcg gcaaccctgt tgatttccga ataaagaccg gggcgaactt ccgccgagtt
     6061 ggtatcgaag gtcacatcgc ccttgaacgt gaccgacaag agattgcctt ctctggtaac
     6121 cgctgcagct tccgacgtcg ccagagcatc gcgcatatcc ctttcctggt tgtccatcat
     6181 tgtgccgaca ccgtagccgc cggcgccgcc gattgcagca ccgattgcag caccgatcag
     6241 cgttgccttg gtatcacggc caatcacctg acccaaaacg gctccggcaa ccgctccgcc
     6301 tgtagcacct accagccccc ccgtctgact ttttgtcatc gtgcctgtct gtgcgcaacc
     6361 ggtcagcatc agagttccgc acagaaaaaa gcaaataatt atctttttca tttttgtctc
     6421 cttcttaaac ttaatttgtt tttttaatcg tgaaacgttt gaagagtata tgacctccgc
     6481 gctggtgcgt caagcgaatc ttatcgggga gagcccttgt aatgacacga tggacgccga
     6541 tgacaggcca tgaatataat ttaaaatttg cggctgttga tcttttttgt tattgcacag
     6601 gaatgaatca tgacatagcg tttttgaaaa accaggcccg gcaattaaat atcgctcttt
     6661 cgaacgggca actgcagcag ttcgacgtct ataaaaacga attgatggac tggaacgcca
     6721 agacaaatct gatttctgaa aaatcggccc gcgatattct tgcccgacac ttcctggatt
     6781 ctcttacggc cttgcggtac atcacaccgc ctgatgcaag aatcattgat gtgggaagcg
     6841 gcgccgggtt tcccggtctt cccttaaaaa tagcgcagcc ttcgctggac ctttatcttc
     6901 tggaagccaa tcgcaagaaa gtgtcctttt taaaacacat cgtgcggctg cttaatcttt
     6961 cttccgtcga cattattcat gaccgcgttg aaaacatcat ccgagaaaat gtttatcagg
     7021 aaaaatttga cgttctgatt tcccgggcct cgttcaaatt gccgcaactg tgttcattct
     7081 gccggtattt tctcaccccc gcaggccggt tgattgcctt aaaaggtccc ggggtggaag
     7141 cggaaattca aaaatgtttt gaggaccgaa atcaatccgg aatttatcaa ttgattcaac
     7201 agcctataga ggctcctttt ttgaattcaa cgcgcaaaat tattatcggg caaagataaa
     7261 aaatccttct aaaaagcatt tttttatgct agtgctatcc acagttcctg ctaaagaaca
     7321 ttgtttgcga tactacataa taaatgaata aaatcatatg catagcgaac cagaagggtg
     7381 gtgtcgggaa aactacgact gcaataaatt tatccgcctc tctggctgcc gcggaaaaaa
     7441 aaacgttgct gattgacggc gactctcagg gcaattccac aagcggtgtc ggggttgaca
     7501 gagcggtgtt ggagacggcc aatttgtatc atgccatgat cggcgatgtg ccgctcaaag
     7561 acgtgatcat gcacacggca attccgcatc tggacgttgt cccgtccaca caggatctga
     7621 tcggcatcga agtggaattt gtcggtctgg aagataaaga aaaacggctg aaacagttat
     7681 tgaacgatct ggacgcgcgt tatgatttta tcgtcatcga ttgcccgccg tccctgggcg
     7741 ttatgacagt caacgctttg gtcgcagctg attatttgat tgttccttta caatgtgaat
     7801 attatgcgat ggaaggtctg gggtatcttc tcaacaccgt taaactggtc aaggcgcaat
     7861 tgaatcccca tctggttttg ggcggcattc tgctcaccat gttcgatcag cgcaatttgc
     7921 tgtcgcatca ggtgtccgat gatgtgcgca aacatttcgg tgacaaagta tttaaaacgg
     7981 tcattccgcg taacgtgcgg ctctcggaaa gtcccagtca cggactgccc gtcattcttt
     8041 acgatattaa atcgcgcggc gcgatggcct atatggaaat ggccaaggaa atcatccaag
     8101 ggggattgta aatggcacaa aaaaatgtgc tgggaaaagg cttgggcgct atatttcccg
     8161 atttaatcaa caatgaatcc gataagaaaa aaagtttgta ctgcggcata gaagaactcc
     8221 accccaatcg ttatcagccc cgtaaaactt ttcatgatga taaacagaag gaattgatcg
     8281 cctcgatcaa aagcagcggg atcattcaac cgattatcgt ccgtaagacc aatagcgggt
     8341 acgaaattat cgccggcgaa cggcgctgga gggcggctca ggctgcgggt ttaaaagatg
     8401 taccgattgt aatccgtgaa gccagcgaca gggaagcggc agaactttcg ctgattgaaa
     8461 acattcagcg ggaagaatta aatccgcttg aagaagccgg cgcttatgtg accctgatgg
     8521 accaattcaa gctttcacag gaggacattt ccgcacgggt tggaaaagac cggtcgacga
     8581 tttccaacac cattcggctg ttaaaattgc cggctaaaat acaaagcgct ctgatggcca
     8641 atcagatttc ggccggtcat gcgcgctgtt tgctggcatg tccttcaccg gaagaacaga
     8701 ttgccgcttt gaccctgatc ttaaaaaaag atttaaatgt ccgggaaacg gaacggctga
     8761 tacaacgggc gaaaaaaatc ccgctagaca aaaaacagcc tgttaaaaaa gaccggtttc
     8821 tcaacgattt ggaaaaagcc atgacggtaa aattacaggc caaagtccaa atcaacggat
     8881 cggacaaaaa agggctcatt gaaatccggt atacgtctat ggacgaattg aaccgcttgt
     8941 ccggtttaat tcttgacgat ttacagtaaa tcaggttaat taggtatatc aacagctgtg
     9001 gatatgtttg ttgataaagg gtttaatatg gacgcgtttt gggacaaaac tagtaaaatc
     9061 attcaggaaa aaataagtaa gcagaatttt gacacgtgga ttaagcccat tcaaattgtg
     9121 gcgatggaag agcagtgtgt caagttggcc gtaccgaata aatttttcag ggactggctg
     9181 gtggataatt attcctcaac cattaaacaa tcgctaaagg acgcttccgg tcttgatgtg
     9241 aatattgatt ttatcctttc caaaaccaaa gaaaccgatc acgagcccaa agcaaaacag
     9301 accgctgccg ccaaaacggc tgctccggca gtccatagcc gcagtaaaac cttttctttt
     9361 ttaaacgata attacagttt tgaacgattc gtggtcgggt cctgtaatca atttgcccat
     9421 gccgcctccg ttgcggttgc caagcagccc gccaaaaatt acaatcctct ttttatttac
     9481 ggcgggtcag gacttggaaa aacacactta ttaaacgcga tcggtctgat ggccaattcc
     9541 attcatccgt cactcaatat catgtatgtt tcggcggaag aatttatgaa tgaaatgatc
     9601 aactccatcc gttacgacag aatgcccaag tttcgggaaa aataccggaa tatcagctgc
     9661 ctgttgattg atgacattca tttcctggcg ggcaaagaca gaacccagga agaatttttc
     9721 catacattca acaccctgca cgactccgga aaacaaattg tcgtcaccag cgacaaattt
     9781 cccaaagata ttcccaacct ggaacataga ctgcgttcac gcttcgaatg gggcctgatt
     9841 gccgatattc aaccgcctga aattgaaacc aaaattgcca tcattgaacg gaaaattcag
     9901 gaaaataaaa ttgaactgcc caataatgtc attcactaca tcgcgtctca tgttgagtcc
     9961 aacattcgcg aactggaagg ttttctggtg cgtatcggcg cttattcctc gctgacaggc
    10021 cgggaaatcg acctggattt agttaaggaa gttctgagta atctcgtgaa acacaatcat
    10081 aaaggggaaa ttagtattga agagattatg aaggtcatct ccggaaaatt aaatattaaa
    10141 atctcggata tcaaagccca taataaaaat aagaatatcg tttttgcacg tcagattgtt
    10201 atgtatctag ccagaaaact aaccaatcaa tcttttcccg acatcgggca gaaaatcggc
    10261 ggtcgtgatc actccacagt gatttatgca aacaataaaa tagcaaacaa tataaaatcg
    10321 gatataaagt tgaaaaacct tattcaggaa atagaggata ttttaatcca caaataatcc
    10381 tcttttaata caaaaattat aaacaggcgg aagaaaaata taatatgaat ttaattaaat
    10441 aaaaaacatt tatcaacata taaacaaact atactattaa tactattttt ttatttataa
    10501 aaagaattac taaaaataag gaggctatat ggaatttaaa atcaatcgag aaatctttct
    10561 tgatgggatc cagaaaactt taggtattgt tgagaaaaaa accacaattc ctattttaaa
    10621 taatatcctt attaaagctg aagccggtaa aatcaaaatt attgccacag atagagagat
    10681 tattttaatt tccgattacg aagcggaagt gatggaaaag ggagaaataa ccatttccgc
    10741 caagaagata tacgaaatga tccgtgaaat acagggagag tttattaatt ttattcagaa
    10801 gaataatctt gtgaagatat ccgcgcaaag agctttttat aaaataccgg gattgcctgc
    10861 tgatgatttc ccgagtattg ccgatgatca ggatataccg ttttccaaga ttcagggaag
    10921 tattatcaaa gatctaatca acaagacgtc ttttgccatg gcgacggatg agacaagaaa
    10981 aaatttaaac ggtgtcttga tggaagcggg actggatggc tcgaattatt tattgcggat
    11041 ggtggcaacg gatgggcatc gtctggccat ggccagggga tttacaccgg agccgacgtt
    11101 aaagacggtc aaagggatta ttattccccg taaagggttg atggaaatca aaaagattat
    11161 tgacgagaat aaagaggttc agatcggcct tcataaaaac atgtttctgg tcaaaacgga
    11221 aaataccctt ctgaaggtca gtcttgtgga cgccgattat cctgattata aaaaagtgat
    11281 tccaaccgaa aaggggatga gtgttgtact ggaaaaagac tattttctgc atgcgttgag
    11341 aagaatgagt gttgtatctt ccgaacggta cggcggagtg attctttctt tttccaaggg
    11401 aaaattaaca ctgaattcga cgaatctgga tgttggagaa gccacggaag aaatcgatat
    11461 cgattatggt ggcgaggcga ttgatagtgg atttaatgtc aactatttaa tcgatgccat
    11521 cgctgtcgtc aataaagaga atattgtttt tgaagtcggt ttgggattga aaccgtccat
    11581 cattaagcag gcggaagatg ataattattt atgcattatt atgccgttga aaatataatt
    11641 tcagagagga agcagaaaat caaacatgac acataattat gatgcggata gtattaaggt
    11701 attagaaggt ctggaagctg ttcgtaagcg gccggcaatg tacataggtt ccaccggaaa
    11761 agaaggtctt caccatcttg tttatgaagt cgtcgataat agtattgatg aagcagtggg
    11821 tggtttttgt gataccatca ccgttaaaat cagggtggat aacagtattg tcgtagatga
    11881 caatggccgc ggtattccga ttgatatgca taaaacggaa ggcgtatctg cggcagaagt
    11941 cgtcatgacc aagctgcacg ccggcgggaa attttccaat gactcctata aaatatccgg
    12001 aggtctccat ggcgtgggcg tgtccgttgt gaacgcgctt tccaaaacct gcgagctgga
    12061 agtgcgtcgg gacggcaatg tttatatgca atcctacaaa cgcggtattc cggatgctcc
    12121 tctggaaaag atcggtaaaa cccgggggaa gggcaccaaa gtcacttttt taccggatga
    12181 tgaaatcttt gaagaaacgg aattcagcta cgatattatt gccaatcgtt tgcgcgaatt
    12241 ggccttttta aattcaggcg ttaaaattat tctcattgat gaacgtgcca acaagcagaa
    12301 tgaatttttc tacaaaggcg gcatcatttc ctttgtggaa tacatcaatc gcaataaaaa
    12361 agttctgcat aaaaatccga tttatgtttc aggcgcacgg gaagactgct ctgtggaagt
    12421 agcgctgcag tacaatgaaa cgtacctgga aaacattttt tcatttgcca acagcatcaa
    12481 caccaccgaa ggcggaacgc acatgatcgg atttcgttcc gctctgaccc gcgtgttcaa
    12541 taattacgcg accaacaata aaattctcaa agacggcaaa gaatcgctgc gcggcgaaga
    12601 tcttcgggaa gggctggcct gtgtgatcag tgtcaaggtg aaaaatccgc agttcgaagg
    12661 tcagacgaaa accaagctgg gcaacagcga agtccgcggc ctggtggaag gcatcatcta
    12721 cgataaaatc ggcagctacc tggaagaaaa cccctccgtg gccaaacaga ttctcaacaa
    12781 gtgtatggaa gcggctcggg cccgtgaagc ggccagacgg gcccgggaac tgacccgccg
    12841 taaaagcgcc ttggaagtcg gcgcgctgcc gggcaaactg gccgattgcc aggaaaagga
    12901 tccggcgctt agcgaaatct atctggtgga gggcgattcc gccggcggtt cggccaagca
    12961 gggccgggac cggaagaatc aggccatcct gccgttgcgc ggcaaggtgt taaacgtgga
    13021 gaaagcgcgt ttcgataaaa tgcttcagaa tgaggaaatc aaaacaatgg tgacggcttt
    13081 cggagccggc atcggcgccg acgattacga catcagtaaa ttgcggtatc ataaagtcat
    13141 catcatgacg gatgcggatg tggacggcgc gcatattcgc acgctgcttc tgaccttttt
    13201 cttccggcag atgcgggagt tgattgaaaa agggtatttg tttattgcgc agccgccgct
    13261 gtttaaagtg gtggaaaaaa agcaggaatt ctatctgcag aatgaagagc agatgaaaaa
    13321 ctatattctg gaaaacggcg tgggcaaggt caagctgatg atgggcgacg gctcgccgtt
    13381 tccggtcaac ggcaacaagc tgctcaccct gattaaaaaa gtcatgcgga ttaaagacca
    13441 gatggaacgg tttgaaaagg aaggccggga cggcaatctg atccgtattc tggccggcga
    13501 cccaaccctg accgatgaca gttttaagag cgaaaaagcg cttgcggccg tcgccaaaag
    13561 aaccggcatg gcgctgggcg agagttttgc cgattttcaa atcgaaaccg atgaagatca
    13621 cggcggcttt aaaatcgttt tcaatctgaa gaagaacgga cacactgttt catcctgcat
    13681 caaccgggag gtcttccgta caccgcagtt cgaggatatc cggttattgc tggatcaggt
    13741 ggccattatc ggagaaggtc cctacagcga caagggcgat gaatcgtcaa cgccggttgt
    13801 gctggaaaac cccaaagcgc tcgtagatta cattgtcagc gtcggcaaga agggcctgac
    13861 ggtgcagcgt tataaaggtt tgggtgaaat gaatcctgaa cagctctggg aaaccaccat
    13921 gaatcccgaa aagcgcacgt tgctgcaggt tacagtggaa gacgcggtcg aggcggatac
    13981 aatttttacc acgctcatgg gcgatcaggt ggagccgcgc cgcgatttta tttatgcaaa
    14041 tgccatgaat gtttccaatt tggatatcta gttagcaatt ttagcatttg ttttgaatat
    14101 ggactgtgcc gtctgtgata aggccggttt tcttatagag aggaagggaa aaaatggaac
    14161 gtgatatcca ccacaagcaa acgctggtga atatagaaga tgaaatgaaa aaatcctaca
    14221 tggattattc catgagcgtt atcatcggcc gggcgattcc cgatgtgcgc gacggactca
    14281 agccggtgca ccgccggatt ttatattcca tgtatgaaac aggctaccgc cacaataaac
    14341 cctacaagaa atgcgcccgt atcgtcggcg atatcatggg taagtaccat ccgcacggcg
    14401 acgcggcgat ttatgacacg ctggtgcgca tggcgcagga tttttctctg cgctacatgc
    14461 tggtggacgg ccagggcaac ttcgggtcga ttgacggcga tcctcccgcg gccatgcgtt
    14521 acacggaaag ccgcctggcg cgcatttccg acgaggtgat ggccgatctg gaaaaagaga
    14581 ccatcgattt cgttcccaac tatgatgaat ctctgacaga gccgtcggtg ctggctacgc
    14641 gcattcccct gctgctgctc aacggcacat cgggcattgc cgtcggcatg gcgacgaata
    14701 ttccgcctca taatatagtg gaaattgtca acggcaccat cgcctgcatc aagaatccag
    14761 cgatcaccat tgaacagttg atgcgtcaca ttccggggcc tgattttccc accgccggat
    14821 ttatcaacgg ccgcgaaggc attctgtccg cgtataaaac cggccgcggc atcatcaaaa
    14881 tccgcgcgcg cgcgctgatt gaaaagaacg gccgtaccga caaggaaagt attgtcatta
    14941 cggaactgcc ttatcaggtc aacaaggcaa tgttgattga aaaaattgcc gaactcgtgc
    15001 gcgacaaaaa aatatccggc attgccgatc tgcgcgacga atccgaccgc gaaggcatcc
    15061 gcgtggtcat tgatctcaag cgtgatgaga attccgcgat cattctcaat cagctctata
    15121 aattcacgca aatggaagtt tccctgggca ttatcatgct ggcgatttcc gacggccgcc
    15181 cgcaggtttt caatctgaaa gaagttctgg agaaattcat tgccttccgg caggaagtgg
    15241 tcatccgccg cacacagttt gatctggcgc gcgccaaaga gagagcacac attttggaag
    15301 gtctgattat cgcgctcaac aatatcgacg cgattatcaa agtcatcaag gcatccaaga
    15361 cccccgcgga agcatctgta caattatgcg ccaaattcaa attgtcggaa atccaggcca
    15421 aggccattct ggaaatgcgg ctccagcgcc tgaccggtct ggaacgggac aagatcgatg
    15481 cagagtacgc cgaagtgacc aagctcattg cttatctgca agggattctg gatgatccgc
    15541 agaaagtgct tcaggtcatc attgacgagc tcaacgaaat taagcaaaaa tacggcgacg
    15601 aacgccgcac ggaaatcgtc gccagctcgg aagacatcaa tatcgaagac atgattgtgg
    15661 aagaagacgt ggttgtcacg atctcgcatg ccggctacat caagcgcaac gcggtcagcc
    15721 tctacaagag ccagcatcgc ggcggccgcg gcaaggtcgg catgggcacc aaggaagagg
    15781 attttgtcga aaaaatcttc atcgcctcca cgcataatta tctgcttatc tttaccagcg
    15841 ccggcaaggt gtactggctc aaagtctatc agattccgca ggccgggcgc gcagccaagg
    15901 gcaaggccat tgtcaatctg gtcaacctgg cccccggtga aaaagtcatg gcaaccctgc
    15961 cggtgcggga atttgtcgat gacaagttta tcgtgatggt gaccaagaaa ggcaccatca
    16021 agaaaacgga acttaccgcc tatgccaatc cgcgcgccgg aggcatcatt gccatttcca
    16081 tcgatgaagg cgatgagctg gtggaagtgc agttgacgat gggcaaccag gatgtcttta
    16141 tcgcgacaaa acagggcatg gccatccgct tcaaggaaga cgacgtgcgc gacatgggca
    16201 ggaccgcccg cggcgtgcgc ggcatcagcc tggatgaagg cgatgaagtg atcggcatgg
    16261 atatccccgc ccagaataca ttcatgctga ccgtttccga aaacggtttc ggcaaatgca
    16321 cgccggtaga tgaataccgc gtacagtcgc gcggcggcaa aggcacgatc aatctgaaga
    16381 ctgtcgaaaa agtcggcaat gtctccggcg ttctgcaggt gatcggcgaa gataacgtca
    16441 tgctcatcag caatgccggc aaagtcatcc ggctcaaggt gcaggaaatt ccggtcaatc
    16501 accgcgtcac gcagggcgtg aaactgattg atctggagcc ggaagaaaaa ctcgtcggcg
    16561 tcgcccgcac cacatcggaa gccgaaagcc gcgacgacgg caacggcgat gacgaaggtg
    16621 atgcgaacgg cgaagacaac ggcgccgaag aatagtaatg agccaagctg ataatgagac
    16681 tcgttgagat cgaatgaagt gaagatcgaa acgttagtcg aattacacca ggtcttgcac
    16741 cgtggtgaag cgaacgtagt gacgctcgaa gcgtaaggcg aactatccca ggagcaacgc
    16801 gacgagggag aatagctccg ccgttacaat agaagtctta atcacgggta caatagtgcc
    16861 ctccggtctg cgggattgtt cgacttacaa atttcaatgc acgacgcttt tgaaatttga
    16921 gtctcacaat aaataagttt ttataaaaga ataatcaggg aacacaagtt tcagcaacgt
    16981 aagggctgaa acttgtgtgt ttcattgttg cgcaaaacgg agattagaag accattgtta
    17041 tattaattca ttaaaaccat gtttgccatt agggagaaaa aatatggaac ttatttttca
    17101 aggatcaccg aaaatagtcg tcgcaacaaa cacttttatc aatgtacccg ttattctgaa
    17161 gtatgaagac accccactat tagaggttat taaggaacag ggcattggtt tcatgacaca
    17221 aataccgatc taccattcag acggaacata tttagcaaag gttaccggta atcgagtgta
    17281 ccctacagaa gagggaaaga aagcaaaaat cgaaataaga gacaatcccg gtaaattcgt
    17341 ctgcagtctt gacaaaagag tgttatttga actttctcat ggtgtcggtg atgcgttcaa
    17401 agcaacagct gaattatata caccggatgg atgttttgtt aaatgcacgg ataaccctaa
    17461 gccagagcta ataaacttag caggtaacac aatgagagtt ggaggcgtta cgatgatcgg
    17521 gaacgttttt caaaatctct ccataggcat ttggttgcgc aaagatggtt catgtgcaat
    17581 aggtgtagga tagaatatct aagggtaaat tgggaacata acatttaatt ggttgctgcc
    17641 ccgtggtagc accgatccaa ataaacaaag ctaaccttgg cgaatcatga attttgagct
    17701 tgagccatat catcgtgaca ttacgaaaga gcaagtcgtt aacgatatta ccgctgttgc
    17761 acgccaactc ggacaatcta caatcacaac agatgattac cgccgccatg gtaaatacag
    17821 tccagatgtc gctcggcggc gttgcgggtc atggctcaaa gctttagtag aggcgggact
    17881 ttctactcaa agacacaata cgaaagccgc acccgaacaa ttcattcccg accttaagcg
    17941 tgtagccaca ctcctcggca agccatcggt cacgaccgag gaatatcgcg accatgggcg
    18001 cttcgcgcct tctactgtct caaaccattt cgggacatgg tttgccgctc ttgatgcagc
    18061 tgggctaaac cggactcgaa ctctacatgt cacagatgag gagtattttg agaatcttga
    18121 gcaaatgtgg gtacatcttg gtcggcaacc gcactacacc gatgtgcaga aaccgttttc
    18181 ccgctattcc gcaggagcat atgagaaacg gttcggatca tggcgtaaag cactcgaagc
    18241 ctttgtagtc ttcgtcaacc aggaatctga taaaaccaga caatctagac cacctgcatc
    18301 gaaaaagaaa aaagagtata ccatgccggc tgttcagcaa aaaccatcgc gctacatttc
    18361 ttggcgttta cgttttcttg tcatgcgtcg agatgatttc aaatgtcgta tttgcggaag
    18421 aagtccagca cttcaacctg gtttggtgct acacgtagat cacgtcaagg catggattaa
    18481 agacggtgag actgttatgg acaaccttca aacgctttgt gagcagtgca atgttggtaa
    18541 gagtgatcta tccatgaaaa aggaaggcta accaatcact aaagccgaca gtgcttcgcg
    18601 cctctgaagt aacttttcga tatgctttta acatcccaaa aaagacctaa gaaaaggaat
    18661 aacatcagtg gatgacaaat ctaaaaatcc tttagaaatg ataaaagacc catatgcagg
    18721 tgatagcttt atccttcctg atacgctatg cacagatcaa agaagatctt ttttccgtcg
    18781 atctaaggaa atattaaagt tccttactat ttcagatctt gcattaaaag aatgtaaaaa
    18841 cagatatgag caacttcttt cacaaaaaaa tcttcaacaa gatactcctc ttaaatttgg
    18901 attctcagat ggccgatcaa ctataatgcc agtgagtagt tttttaaatg ggtgcaatga
    18961 aggtgtcgaa ttgttatgtc gtcaagtatt tattatgatt tatggcgctt gggaaactta
    19021 tttgtttgaa atactcgagc aatcttttcc taaaatcgga atatctgaaa atatattaga
    19081 gcattctctt aaaataatga tgaagggaaa ttgggatgga aagttctgta agatgcaaaa
    19141 caaccttggt attgcttaca aatctggcga tttagtcaat cactttaatg gatttgaatt
    19201 gagttatgga ggtacgatat tcgaaaatcc tttacttttt ttggacgaat tagctaaagt
    19261 ccgtcataga attattcatg cctcaagtat gttagataac ggaaagtcaa tttttattaa
    19321 cgctcaaata tttccagcgt atctagcatt tttcacgctt ctaacagaat acgtcgataa
    19381 attattttca gagaaattcc agtataggca ggttaagatt aatccagcag aaacttagaa
    19441 tcttgatctc cttgttcaat aagcaagggt aaaatgggga cggttctatt taatgataat
    19501 ttaacatacc gataatgccg attgttaaga ttccgtttag cttactatga aagcgaccta
    19561 atcagttagc tccacttgtt caataagtaa tattcagtta tactctcctc atgtcgaact
    19621 acaaatattt tgatcatacg gcggatatcg gcgttgaagt tacaggccgg accagaaaag
    19681 aactgtttgt gaatgctgcc gaggctttgt ttgatgtaat gatcgaaggc aaatttgttc
    19741 ggaaagctgc aaaacggcag aaaaaaataa ccgtagaggg agcggatgtt gcagatttgc
    19801 tgattaattt tttgcgggaa ttgctttatc tgtttaatgg cgagcaattt ataacgggca
    19861 attgtgaaat tatagaattt tcaaataaaa aaatacaggc acggttaacg ggaggatcgt
    19921 ttaacaacaa aaagcatttg attaaaacgg agatcaaagc ggtaacctat tccggtgcaa
    19981 aggtggagaa aatagaatca ggttggaagg cgagagtcat ttttgacgtg taatagcaac
    20041 cttagatgtc atgccggcgc aggccggcat ccagtattat ttattcctgg atttaccctg
    20101 aagggcacta tagccggcct tcgcgggaat gacaaaatag aagtaaaagc cttagaagag
    20161 aaattgttat ggctgaaata aaattggaaa aattggatga caatcgctgg ctgctgcctc
    20221 aggcgggcgg catgagggtg ccggggatta tctatgccaa tgaaaaaata tatcagcttt
    20281 taaagggtga cgaaagcgcg aagcaggtgg ccaacgtggc gcatctgccg ggtatcgtga
    20341 acttttctct ggcgatgccc gatgtccact ggggttacgg ttttccaatc ggcggtgtgg
    20401 cggccttcgc tctggatggc ggcatcattt ctccgggcgg tgtcggctac gatattaatt
    20461 gcggctgccg tctgatgacc acaaatttaa cactgggcga tatcaaagac cggctcagtg
    20521 atttaacaac cgccctttat cagcgcattc cttccggtgt cggttcgacg ggttcagtta
    20581 aattatccgg taaggatcag aaaaaagttc ttgaagaagg ctccgcctgc gcgatcaaag
    20641 ccggcttcgg tttaaacgaa gacttggaaa ctacggaaga cggcggcgcg atatctggag
    20701 ctgatcccga actggtttca gcccatgctc tggaacgcgg caaacagcaa ctgggcacgc
    20761 tgggttccgg gaatcatttt ctggaaattg aagtggtgca ggaaatcttc gatgaagaag
    20821 cggccaaggt ctttggtttg cacaaaggac agattgccgt catgattcat accggttcgc
    20881 gcggcctggg gtatcagatt tgcgatgatt atctggcgtt gatggtaaaa cacgccagcg
    20941 aaaccggtat agcgctgccg gacaggcagc tggcctgcgc ttatcttgat tcgggccggg
    21001 ggaaaaatta tctggcagcc atggcctgcg gagccaacta tgcctgggcc aaccgccaga
    21061 tactgatgca caaaacccgg gaagtttttg agaaaacatt acggatgtcg ccacgcgaag
    21121 tgaatatgcg gctggtttac gatgtctgcc acaacattgc caagatagaa tcgtttaata
    21181 tcggcggtgt gctgaaaaaa ctttgcgttc atcgcaaggg ggcgacccgt gcttttcccg
    21241 ccggacatga ggccgtacct gaacgctaca ggaaagtcgg ccagccggtt ttgattcccg
    21301 gcgatatggg aaccggttct tatgtgctgg tcggtcagga aaaagccatg gcggaaacct
    21361 tcggcagcgc ctgtcacgga gcaggccggg tgatgagccg gtcgcaggcg atcaaatcca
    21421 gccgcgggcg ctccattgct cgcgagcttt cggaaaaagg tgttttggtg atggcgggtt
    21481 ccaagggaac gctcagcgaa gaaatgcccg aagcctataa aaatgtgcag gatgttgtgc
    21541 aggtgatgca tgaggcggga attgcgaaga aagtggcacg gcttaaatcc atatcgtgta
    21601 ttaagggata atgccttacc cctggaaata ttttgcatgc tgatgtaaag gtattattac
    21661 atattgtaaa aaagttcctg attgtcatac cagcgaaggc cggtatccag ggaaaataac
    21721 aacataaaaa agccttgcga actttagtat atgatgttgc agcagcgtac agcagttata
    21781 aaaacactgg attccgtatc gcgcttcgct tgtacggaat gacttcaata cactagggat
    21841 aatgtaagaa ccggagacct tatgacggaa atcatttttg atgaaaatga agattgggaa
    21901 aagcgaatat tgtgcagcga tgagtcctgt atcggcacca tcggtccgga cggcaagtgc
    21961 aaggaatgcg gcaaacctta cgaaggaaaa ttgc
//
LOCUS       DAOOLB010000032        21794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_2331_length_21794_cov_35.645246, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000032 DAOOLB010000000
VERSION     DAOOLB010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 21794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21794
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2331_length_21794_cov_35.645246"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PLF77_05710"
     CDS             <1..931
                     /locus_tag="PLF77_05710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC08387.1"
                     /translation="INSTEDYHKNELNSLGWELTVCNALDPMDSPCRRLIASQTSFGE
                     NLYRFLEEIIPVDTIRTILEIGGGMGYLMRDFLKFNPRLKATMIDISPYLLGCQKDLL
                     HSFEVDFLLADILTVPPTTLSSFDMAVMNENLGDLPTLVTGSKDDIYHGPAAGVIEKI
                     EAFNIKYNLQLGANQNEPVNIGALEMVEKLCLAGIKYIYLSEHSCEAAVPEKMKNYLF
                     FQTPGIPEMISLKGHREFTIKFSYLQKIAEMLNYTSRRGPFADFLPLDFNDRVKTALR
                     MLTPQSDNQEIIRQFVYDLFKYEYLILIRGDHK"
     gene            928..1266
                     /locus_tag="PLF77_05715"
     CDS             928..1266
                     /locus_tag="PLF77_05715"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HPC08388.1"
                     /translation="MKKTVMIIFPALFFLLSVSVSPCHAFRCGNGLVTTGDTKNKVQM
                     TCGKPSAKEKRCLNSWTGKKAKCDKKVEVWYYNCGDNDFVYALIFEGNTLTGENSEGR
                     GHGPSECRGQ"
     gene            1570..3348
                     /locus_tag="PLF77_05720"
     CDS             1570..3348
                     /locus_tag="PLF77_05720"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HPC08389.1"
                     /translation="MTMDMLIVFGIILLAALLFASEKLRVDLTAIVIMVLLILTGIIS
                     PSEGISGFSNVATITVASMFILSAALQKTGSVNFLGTISSKIFRFNFWVGLIGTMIFV
                     GAASAFINNTPVVAVFIPIMLGVAADNQMSPSRLLMPISFASMFGGVCTLIGTSTNII
                     VSSVAVQHGLPALGMFEFTKMGLVFFAAGLVYMAVVGVRLIPLKKTETDLTKKYQMND
                     YLTDIVLLGNAKSIGKRVADSPLIKELDIDILEVIRNGRRLHVPVSDIVLMENDLLRV
                     RCDIKQLQKLKERMGIKMKSDCELNDENFRCEALSLTEVVVAPNSRLEGKTIKSSYFR
                     NVFHATALALRHRGQLFNTGFTDTILRAGDAILVETRKENYEALKNSNDFVIVSDIET
                     QQYRKSKIIPALLVIAGVIVTASLGIIPIVVGAMIGAILLVLSGCITLEEAYDAIDWQ
                     VIFLLGGVLSLGIALEKTGAAMYLSNNLIYVLGDLGPVAIVAALYLLTSILTELMSNN
                     ATAILLAPIAIAAANGIGVSPTPFLMAIAFAASASFMTPVGYQTNTMIYGVGQYRFSD
                     FIKVGTPLNIMFWILATLFIPVFFPF"
     gene            3357..4226
                     /locus_tag="PLF77_05725"
     CDS             3357..4226
                     /locus_tag="PLF77_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPC08390.1"
                     /translation="MDGITALINQILNVFRIPLFHMGEAPFTLWTFIYLVAATMILTL
                     AASVLNKVIVTRLLTKSNIERGVRIAIGSIVKYIVLVVGFIIILQTAGINLSSLTILM
                     GALGVGIGFGLQNITNNFVSGMIILLERPIKVGDRIEVANVSGDVVDISMRATTIVTN
                     DNISIIVPNSQFISETVINWSHTDRSVRFNFPIHVSYREDPQAVRKLLLDIALNNPGV
                     LKDPPPDVLFSEFADSAMSFNLRVWTREYINRPGVLKSQLYYQIADRFREKGIEIPFP
                     QQDIHIKETARQS"
     gene            4452..5069
                     /locus_tag="PLF77_05730"
     CDS             4452..5069
                     /locus_tag="PLF77_05730"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC08391.1"
                     /translation="MVTLWLITTGCSEKLPILYSPLKDDYQLSPGFTDREQGEKQLDE
                     FFEEDQRLYRNLSRLTTVIDDAEDDPGDEAEIHSYPEAHNGILEGLTATEWFTAGRQA
                     ASDRRWDDALNAFNKAIALNPENEEIYFHRGNVYDALGNNKKAVSNFNKAIKLNPLYV
                     DAYLSRAFSYNNLAQANQALADIKKAARLGSLHAQKFLKKSNLAW"
     gene            5347..6294
                     /locus_tag="PLF77_05735"
     CDS             5347..6294
                     /locus_tag="PLF77_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPC08392.1"
                     /translation="MRKTIIATVMMMLSAFFITTAQADIRPGSYTVTPFIGGFIFEGN
                     EYYRGFDNQKNGRNTLDDTYAVGVRAGYNFTENLGLEGFFSYIPTSRDNFICCDRKKD
                     NDLDLYYYGIEGLYHFMPYGRLVPFVAVGLGGIRYDAPSGEDNGNKFAVDYGAGVKYS
                     VTDKIDLRADVRHVLPLNERYNDLLYSFGVTFSFGGAEKGKKLLDIKFDFDKTDIKAA
                     YHKDINAVTKLMQKYPDLNIVTEGHTDNIGTPEYNKNLSNKRADAVKKYMVEKGGVAA
                     GRIDPKGLGEGSPAVSNDTEAGRAENRRVEAAVDYTGKK"
     gene            complement(6440..7141)
                     /locus_tag="PLF77_05740"
     CDS             complement(6440..7141)
                     /locus_tag="PLF77_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC08393.1"
                     /translation="MIKKEMIYRNPLVKLGYDNEDILSTGGFGAVLAYAGVGKTALLV
                     QLALNMMLREHSVLHVSLNDAVSKVDLWYQEIFHDIAEKFGIAEVNEYWDKIQPYRFI
                     MTFKVEGFSAPKLEERLTDLMEQNIFKPHTVIIDGFRFDEAGRGQLVQLKELAGKYSM
                     GLWFTVHTHRHEPPQENGLPLSFLHIADLFEVIVQLATKGDEVYITSVKGQPKNSAQN
                     DLLLDPATMLIKESQ"
     gene            complement(7224..9311)
                     /gene="fusA"
                     /locus_tag="PLF77_05745"
     CDS             complement(7224..9311)
                     /gene="fusA"
                     /locus_tag="PLF77_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPC08394.1"
                     /translation="MKIDDQKIRNIGISAHIDSGKTTLTERILYYTKRIHAIHDVKGK
                     DGVGATMDSMDLEKERGITIASAATYCEWKGHEVNIIDTPGHVDFTIEVERSLRVLDG
                     AILVLCAVGGVQSQSITVDRQMKRYRVPCIAFINKCDRSGANPFRVMSQLKSKLGHNA
                     VAMQIPIGLENDVQGVVDLVSMKALYFDGDNGETVREAEIPSHLLEEAKAKREELIDA
                     ASMFSDELTEAILEEKEVSADLIYSALRKGTLQREITPVYLGSAYKNKGVQPMLDGVT
                     YLLPCPSDIENVAIDMDKNEETVVLNNDTEKPIVALVFKLEDGQYGQLTYIRVYQGTL
                     AKGSTIVNVRTGKKVKVGRLVRMHADQMEDVESLRAGYIGALFGIECSSGDTFTSPDV
                     NLTMISMYVPKPVISLAIVPKDNKSQVAMAKALNRFAKEDPTFKTYVDHETGDTIIEG
                     MGELHLDIYVERMRREYGADVSTGNPRVAYRETITQRADFNYTHKKQTGGAGQFGRVA
                     GYLEPIADTDFVFENKIFGGAIPSQYIAACEKGFKQSMAKGPKLEFPVTGVKVVINDG
                     ASHAVDSSDMAFQAAARGAFKEAYLRAKPVIHEPIMKVVVETPAEFQGSVMGLLNQRR
                     GMIIGSQDEDVMCVIEAQVPLAEMFGFSTVLRSATQGKAQFTMEFTLYRQVPQSIAEK
                     IALEAAQNKKTAA"
     gene            9622..>10071
                     /locus_tag="PLF77_05750"
     CDS             9622..>10071
                     /locus_tag="PLF77_05750"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC08395.1"
                     /translation="MYEQILPNLYRIIIPLPNSPLKDLNSYVIKADNRNLIIDTGFNR
                     GVCYEAMENGLHALEIDLSRTDFMLTHMHSDHTGLVSRLFTETSKVYFSRIDSEVFNE
                     NIDWQAMLDYALHNGFELEELIRAAESHPGFKYRPRKIPDFTMLDDGS"
     assembly_gap    10072..10171
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10172..10754
                     /locus_tag="PLF77_05755"
     CDS             <10172..10754
                     /locus_tag="PLF77_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC08396.1"
                     /translation="FKYRPRKIPDFTMLDDGSMIEIGDYKLQCILTPGHTQGHICLYE
                     EDKQILFSGDHILYDITPHIESWSYEVNSLENYMQSLDKVRNLPVKLVLPGHRNFFTD
                     LKGRIDELKNHHEMRTKEVLDVLGKSTMSAYDIAAGMTWDIDCESWEKFPVAQKWFAV
                     GEAIAHLLYLESKGKVKRHPGEKTDFFSAIRME"
     gene            complement(10862..11251)
                     /locus_tag="PLF77_05760"
     CDS             complement(10862..11251)
                     /locus_tag="PLF77_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC08397.1"
                     /translation="MTNQIKVLAIDDEVDFCYFIKKNLTQSGLFDVVVANNGADGIKL
                     ARKEKPDIILLDLFMPDIPGEDVAAELKDHPATADIPILYITALASNDEIVEGESNKM
                     GDNYIMAKPVRTKKLIETIMKILGQSY"
     gene            complement(11220..12956)
                     /locus_tag="PLF77_05765"
     CDS             complement(11220..12956)
                     /locus_tag="PLF77_05765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08398.1"
                     /translation="MATQIPRKRTIFFIRLMVVITTAYFILLSPVAGKELEIYGYIFI
                     AVYLMTNLIVAYIPEKYFHDNRIFYGFILCDSILLPAGIYFSGYIGSDLYLMFFFIIS
                     LTTMSSRFGYLMINTFLFCLIYLWLLHEKGVLTGPKAGAYLIQIPFIIVIAIFYGYLI
                     TTRLKDKDMRINEVRQRYEQVVQATDVLMSIVDRDGKLKFANDIFARFYGYHEDKRLA
                     GLTIAQVNAEDASEADKLLDYVQSVYQKNDMVRYESYNKKHNLWFANTLGPIRDPSTG
                     DVLDVCVISKDITDRIEKEKKLNNTVELLIKTRDQLIQQDKMAALGRMASGIAHEIRN
                     PLEIIYMGIDYLENNIAHQDPQIDGSIEKIYKAANRADNIINNILSFSRKSEYKITQI
                     PVCQLLDNTLVLANQALHKGHVTVKREYETESLEAAGDYNMLEQVFLNLINNAIDAMK
                     ESTEKRLTIRASGKIISEIGYKTGYRYTDFFRIGEKKIVVEISDTGKGMSKDVQSKIF
                     EPFFTTKSVNEGTGLGLSIVHMIIERLSGTIDVTSEENKGTTFYVNIQPQIKKVVAEE
                     GQYDKSDQSSGY"
     gene            complement(13118..14047)
                     /locus_tag="PLF77_05770"
     CDS             complement(13118..14047)
                     /locus_tag="PLF77_05770"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HPC08399.1"
                     /translation="MKKETLVLKTTDGMQMFVYKWTPDGADAVKGAVQIAHGMAEHAA
                     RYERFGGALTEAGYAVYANDHRGHGRTAGSPDNRGYFADENGWAKVVEDMHLLTGLIK
                     KDYPQKPFFLFGHSMGSFLSRHYAMLYGRELNGLILSGTGGDPGVAGRAGLFIARMDA
                     RLHGKKAKSEIMNKLSFGAFNHAFKPNRTEYDWLSRDHAEVDKYINDPWCGGVFTAGF
                     FCDMLEGLSYINKKDNIAKIPKHLPVYLFSGSKDPVGANTKGVIQIYNALRSAGIDDV
                     TLKFYADGRHEMLNEINRKEVFSDVIAWLDQKR"
     gene            complement(14078..14977)
                     /locus_tag="PLF77_05775"
     CDS             complement(14078..14977)
                     /locus_tag="PLF77_05775"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPC08400.1"
                     /translation="MISQEAKNVMNLMSMMKLPDAGELPIELCRQVLADMMSLSLPPA
                     GCRFEPVNAGGPEALWVKAVSADDTAVILYLHGGGYAMGSPQTHAGFTGLLSELSRLS
                     VLSVDYRLAPENPYPAAIEDSLSAYCWLLRQGVSAESIVVGGDSAGGGLTFATMLKLK
                     ERGEALPAAVFAVSPWVDLAVTGESIEINAARDPMITRSGLDYMAGLYAGHADVRLPF
                     ISPLYADLRGLPPVLIHVGTCEMLLSDSLRIAASLENSGVDCTLRVWEDLFHVFHSVV
                     SLPEAIMATEDIAAFVRNRVGGY"
     gene            complement(15148..20280)
                     /locus_tag="PLF77_05780"
     CDS             complement(15148..20280)
                     /locus_tag="PLF77_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC08401.1"
                     /translation="MKGKNISQPIGLESDFSGPQKLRALMIDDSEDDVLLIIRHMRKG
                     GFAPIYERIETAAAMTQALEEKQWDIIICDYKMPKFSAPSAIALLKQTKLNIPIIVTS
                     GAIGEETAAECMRMGAHDYLMKNNLSRLCPAITRELAEAKIRDRQRVAELQKDAALES
                     LRNSRELYARLVDTMPDVVIRTSLEGTVLFVNDRALRISGYQREEIEGRNMVLFAAPD
                     DHERLMKNAALMLDRKLGPDEYKIVMKDGRIIPFEVNGDVIRDKKGNPFGFVFVCRDV
                     SDRKLAEDKLRESEERYRAIIENIEDGYAELNLKGDFIFVNDALCRIDGYPKEELLKM
                     RYREMMDEETARKVFDVYNKIFKTGGSENNFEYEIFTKERQKKYVDTSVSAIKDSAGQ
                     VIAFRGIVRDRTERKKAEEELRRSEEKYRNILETIREAYFEVDLAGNFTFFNDSVCRM
                     SGFSRDELRGMNYSRLSANDETTRKVYQTFNRVYKTERPSEGYDWPIKRKDGSRISIE
                     ASIMLRKDSSGNIVGFKGVIRDITERKQAEEMLQASEEKYREIFNATSEAIFIHDAET
                     GKIIDVNDSMIRIYGYNSKEEALRCSFADGSGNPEPDTEEKALRLIKKAIQEGPQQFE
                     WLARRKDGTHFWMEMQLMKTDIGGEKLVLAVGRDITERKQAEEAIRENEVKYHSLFDH
                     SMDAILLTRPDGSIIDANPAACRMFGRTKQEILESGRTGLVDTSDPRLEVLLAERART
                     GKFSGRLFHFRKDGTRFTADVSTSVFQDSAGKFKTSMILRDVSDQERMEIALRESEER
                     YRTFINATSAMVYLKDSRFRYILVNQAMEELLGMNLQDIIGKDDFQLMPPETADYFRE
                     NDTRVVQTKSTVVVEERLHDKIYESTKFPVLLENGDVVIGGISRDITERKQAENKLKE
                     TEERYNALFEQSIDLVFVVGFDGQFLDANSAALDLVGYTRDEISSLSIVNLLDDDQIP
                     VALDIIEEIHQYGVQQGLKELRLRHRDGSAVYVETQGATIMSNGEPVAIQGIARDITA
                     RKKAEEELLSLTRRLNDIIEFIPDATLVIDRDGRVIAWNRAIEEMTGITKDAILGKGH
                     YEYAIPFYGRRRPMLIDLLDKRDKKMEALYKYVERGSKIYAESYISGLRDGQGAHLWG
                     VAAPLFDKDGNRFGSIEMIRDVTEIKAKEKSLEESLRGREIAEEATRAKSAFLANMSH
                     EIRTPMNGIIGLVNLALQTGLTEKQKDYLNKIESSAQGLMVIINDILDYSKLEADKMK
                     LEILDFNLEDVMTHISDIMYPNAVQKRLDLLFHIDRDVPLFLKGDPWRLQQVLMNLIG
                     NAVKFTEKGEVVVRVEACARFKRGRKDYIHLNFSVRDTGIGLTKEQMGDLFELFTQAD
                     SSVTRRYGGTGLGLAISRQIVQLMGGTIEATSEAGKGSVFSFMIPLEPGKKKKGGKTS
                     FSGTPFKNKRVLIVDKNPTAQKILADYLEGIGLSVTTAASGSKALKILKEKDNFHLLL
                     VESGTSAQDGIKIVRMIRKNAALAAIPAVMIVSTSFQDDTKTVAEKLGVKYFLKKPFH
                     RSNLLDAMMNIFGQTIKKVSPAVRNPVKAPATEALAGQRILLVEDNFINQQVAREILQ
                     QAGLKVVVAENGKDALGKIRQEQFDLVLMDVQMPEMDGYQATKIIRRNKKFKSLPIIA
                     MTAQAMSGDKEKCLNAGMNDYIAKPISIQNTLSVLSRWLYSK"
     gene            complement(20615..20986)
                     /locus_tag="PLF77_05785"
     CDS             complement(20615..20986)
                     /locus_tag="PLF77_05785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC08402.1"
                     /translation="MDNSKKILVVDDDKVFLKLVENDLTQEGYLVITAQNGKEAISLA
                     KKEKPALLLLDINMPGISGGDVGNILGSDPLTRNIPIIFLTALLTKKEEETRKNMIGH
                     HFFMAKPYDLKALLNEIRKYI"
     gene            complement(20973..21308)
                     /locus_tag="PLF77_05790"
     CDS             complement(20973..21308)
                     /locus_tag="PLF77_05790"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HPC08403.1"
                     /translation="MLIEKKRALREINISEEMFNELLNVFVEQTETALKILKKTVTRK
                     NYEEMRKQAHLISGSAGNLRISGIQKLAKEIELEAMEKREISVIKSHIGKLRTDFEKV
                     KEEVSGGQQ"
     gene            complement(21547..>21794)
                     /locus_tag="PLF77_05795"
     CDS             complement(21547..>21794)
                     /locus_tag="PLF77_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08404.1"
                     /translation="PYELMHPNGAIYVDAKPSGTSIERLRFIARTFPHSVWLNPMPPD
                     EWYYSTTVKAIRQVFTMYELTLDGLEKAVQFLTSSGK"
BASE COUNT         5356 a         5459 c         4885 g         5994 t
ORIGIN      
        1 tattaattct acggaagatt atcacaaaaa tgaactcaac tcgcttgggt gggaattaac
       61 tgtctgcaat gcgcttgatc ctatggacag tccctgccga aggcttattg cctcacagac
      121 atcattcggg gaaaatttat atcgttttct ggaagaaatc atacccgtcg acacaatccg
      181 tacgattctg gaaataggcg gcggcatggg ttatctcatg cgtgatttct tgaaattcaa
      241 tccgcgcctg aaggcaacaa tgattgatat ctctccctac ctgctgggtt gtcagaaaga
      301 cctgttacac tcttttgaag tcgattttct tctggccgat atcctcacgg tgccaccgac
      361 gacattatcc tctttcgaca tggccgtgat gaatgaaaat ctgggcgatc ttccgacact
      421 ggtgaccgga tcaaaagacg acatctacca tggccctgcc gccggagtta tcgaaaaaat
      481 agaagccttc aatataaaat ataacctgca gctgggagcc aatcaaaacg aacccgtcaa
      541 catcggcgcc ctggaaatgg tggaaaaact ctgtctggcc gggatcaaat atatctatct
      601 gagcgagcac agctgtgaag ccgccgttcc ggagaaaatg aaaaattatc tcttttttca
      661 aacacccggc attccggaaa tgatttccct gaaaggacac cgcgaattca ccatcaaatt
      721 ttcctatttg cagaaaattg cggaaatgct aaattacacc tcgcggcgcg gaccgttcgc
      781 cgattttctc cctctggatt tcaatgacag ggtaaaaacg gcattgcgaa tgttgacgcc
      841 gcaaagcgac aaccaggaaa ttatcaggca gtttgtttat gatcttttta aatatgagta
      901 tttaattctt attcgaggag accacaaatg aagaagaccg tcatgattat ttttccggca
      961 ttatttttct tgctgtctgt ttccgtcagt ccctgccacg cttttcggtg cggaaacgga
     1021 ctcgtcacga ccggagacac caaaaacaag gttcagatga cctgcggaaa gccctccgcc
     1081 aaggaaaaac gatgtctgaa tagctggacc ggcaaaaagg ccaaatgcga taaaaaagtc
     1141 gaggtatggt actacaactg cggcgacaac gacttcgttt acgctctgat tttcgaaggc
     1201 aatacattaa ccggagaaaa ctctgaaggc cggggccatg gaccttcaga atgccgggga
     1261 cagtgaacat agcactggtg gctccgatcc tgatagccgg aatttaaaag agctgagcgt
     1321 tgtttcgctt caagatgttt ttcgaatgta cgccgctgtt aagagaaatc cgccgggaaa
     1381 gcgccatgat cgtaacgctt ttataatgaa taatcattga cttgccttca tgatttcttc
     1441 tatactgtct ttgtggaagc aagtccttct ccctgatctc cgaaggataa cgcatgtaca
     1501 aatgcagccg gcgtggcgtc aggtcccgca tacttttgat ccttaaggat atctcaaaag
     1561 gataaaccta tgacaatgga catgttgatc gtattcggga tcatcctgct ggccgccctg
     1621 ctgtttgcct cggaaaaact tcgcgtggat cttacggcaa tcgtcatcat ggttcttctc
     1681 attctaaccg gcatcatttc accctccgaa ggcatatccg gcttcagcaa tgtggccacc
     1741 atcacggttg cctccatgtt cattctcagt gcggcccttc agaaaaccgg ttcggtcaat
     1801 tttctcggaa cgatttcttc caaaatattc agatttaatt tctgggtggg actgatcggc
     1861 acgatgatct ttgtaggcgc cgcatcggcg ttcatcaata atacgccggt tgtagcggtt
     1921 tttattccca ttatgctggg agttgcggca gacaatcaaa tgagcccttc acggcttctg
     1981 atgccgattt ctttcgcgtc catgttcggc ggcgtgtgca ccctgatcgg cacatcgaca
     2041 aatatcattg tgagttccgt cgccgttcag catggattac cagccctggg catgtttgaa
     2101 ttcaccaaga tgggactggt tttttttgcc gccggtctgg tctatatggc ggttgtgggg
     2161 gtccggctga tcccgttgaa gaaaacggaa acggacctga cgaaaaaata tcagatgaat
     2221 gattatctga cggatatcgt gcttctcggc aacgccaaat ccatcggcaa acgcgtcgcc
     2281 gattcaccgc tgatcaaaga gctggacatc gacatccttg aagtcattcg aaacggacgg
     2341 cgtcttcacg tccccgtgtc cgatatcgtt ctgatggaaa acgatctttt gcgtgtgcgt
     2401 tgcgatatca agcagttgca aaagctcaaa gagcggatgg gcattaaaat gaaatcggac
     2461 tgtgaactca acgacgaaaa tttccgctgc gaagccctca gcctgactga agttgtggtg
     2521 gctccgaatt cacgtctcga ggggaagacc atcaaatcct cgtatttccg taatgtcttt
     2581 cacgcaacgg cactggcgct gaggcacagg ggacaactgt tcaacaccgg atttaccgat
     2641 acgattctca gagcgggcga tgccattctg gtggaaacgc gcaaagaaaa ttatgaggcg
     2701 ctgaaaaaca gcaacgattt cgtcatcgtc tcggatattg agacgcagca ataccgtaaa
     2761 agcaaaatca ttccagcact gctcgtcatt gccggcgtga ttgtaacggc cagcctgggc
     2821 attattccca ttgtggtcgg ggcgatgatt ggcgctatcc tgctggttct gtccggctgc
     2881 atcacccttg aggaagctta cgatgcaatc gactggcagg ttatttttct gctcggcgga
     2941 gttctatcgc tgggtatcgc gctggagaaa acaggagcgg ccatgtatct ttccaataat
     3001 ttgatttatg ttttgggaga tctgggacct gtcgccattg tagcagcgct ttatctgctc
     3061 acctcgattt tgacggaatt aatgtcgaac aatgccacag ccatattgct tgcgcccatt
     3121 gccattgctg cggccaacgg gatcggggtt agccccacgc cctttctgat ggcgatcgcc
     3181 ttcgcggcct cggcaagttt catgacgccg gtaggatatc agaccaacac gatgatctac
     3241 ggcgtcgggc agtatcgttt ttccgacttc ataaaggtgg gaacgccgct taacatcatg
     3301 ttctggattc tggcgacgct cttcatccct gtattttttc ctttttagga ggtgacatgg
     3361 acggcatcac ggctttgata aatcagattt tgaatgtatt ccgtattcct ttatttcata
     3421 tgggtgaggc tccgtttacg ttatggacgt ttatttatct cgttgcggct acgatgatat
     3481 taacgctggc ggcgtctgtg ttaaataaag tcattgttac ccgtctcctg acaaaaagca
     3541 acatcgaacg gggcgttcgt atcgccatcg gatcgatcgt taaatatatt gtactggttg
     3601 tcggatttat catcattctg cagaccgccg ggatcaatct cagcagcctc accattctga
     3661 tgggagcttt aggcgtcggt atcggtttcg gtttacagaa catcaccaac aatttcgtca
     3721 gcggcatgat tattctgctg gaacgtccga tcaaggtcgg cgatcgcatt gaagtggcca
     3781 atgtttccgg agatgtggtg gatatttcca tgagggcgac gacgatcgtc accaatgata
     3841 atatttccat catcgttcct aattcccagt ttatttccga aaccgtgatc aactggagtc
     3901 atacggatcg gagtgtccgc tttaattttc cgatccatgt ttcataccgg gaagaccctc
     3961 aggcggtcag aaaactcctt ctggacatcg cattgaataa ccccggcgtg ctcaaagatc
     4021 ccccgccgga tgttcttttt tccgaattcg ccgacagcgc catgagtttc aatctgcgcg
     4081 tctggacaag agagtatatc aaccgccccg gcgtgctcaa aagccagctc tactatcaaa
     4141 tcgccgaccg cttccgggaa aaaggcatcg agattccctt cccgcagcag gatatccaca
     4201 taaaagaaac agcgcggcaa tcctgaaggc ggcacaatca cccaaaaatg atacctgcct
     4261 gtcaagtcct gattgtcacc ggatgcaccg gatcgttatt ttataattga acatcccccc
     4321 gattttgaac gcatccctgt cttgatgaac ggggcaaaca tatgctttac tgtttttcca
     4381 ttttgctttt ttgtgttaca tgataaacag gaattgacga tatgagacct gtccggataa
     4441 ttttattatt gatggtaacg ttatggctga taacaaccgg ctgttctgaa aaattgccca
     4501 ttttatactc tccgttgaaa gatgattatc agctttcccc cggatttacg gatcgtgaac
     4561 agggggaaaa acaactggat gaattttttg aggaggatca aagactttac agaaatcttt
     4621 cccggttgac gaccgtaata gacgatgcag aggacgatcc gggcgatgaa gcagaaattc
     4681 actcctatcc ggaagcgcat aacggaatcc tcgaagggtt gaccgccaca gaatggttta
     4741 ctgcaggccg tcaggctgct tcggacagga gatgggacga cgccttaaac gctttcaaca
     4801 aagccattgc cttaaatccg gaaaatgagg aaatctactt ccatcgcggt aatgtgtatg
     4861 atgccctcgg caataacaaa aaagccgtct ccaattttaa taaagccatt aaactgaacc
     4921 cgctgtatgt cgatgcctat ctaagccgcg ccttttccta taataatctt gctcaggcca
     4981 atcaggcgct tgccgacata aaaaaagccg ccagactggg cagtctgcat gcgcagaaat
     5041 ttttaaaaaa aagcaacctg gcatggtaag atttgtccat gcggacatgt gattttacga
     5101 gttgtccgga ttttgcaaac ggcgtttgaa taacaacggc acggctcatt catccccgca
     5161 cgttattatt gaatttactt gacacacatt ttgaatataa ttatactttc gaccgaaaaa
     5221 gctaaacgcc tgtgaaaaat taaacgaagg gaaatgcttc cgataatttt ttgtgaaatt
     5281 ggaacaggtg atttgttttt caagcaatat atttttataa ataaattcga atcaaaggag
     5341 aacattatga gaaaaaccat tatcgcaacg gtgatgatga tgctatcggc tttttttata
     5401 accacagcac aggccgatat ccgtcccggt tcttataccg ttacaccctt catcggggga
     5461 ttcatcttcg aaggaaatga atactaccgg ggatttgaca atcagaaaaa cgggagaaat
     5521 actttagacg acacctatgc cgtcggggtg cgtgcaggct acaatttcac ggagaatttg
     5581 ggtttggaag ggttcttcag ctacattccc acctcgcgag ataattttat atgctgtgac
     5641 cggaaaaaag acaatgatct cgatctttat tattacggga tcgaggggct ttatcacttc
     5701 atgccttacg gccggttggt gccttttgtg gccgtcggac tcgggggaat tcgctacgat
     5761 gctccgtccg gtgaggataa cggcaataaa tttgccgttg attacggggc cggagtaaaa
     5821 tattctgtaa cggataaaat agacctgcgc gccgatgtcc ggcatgttct tccgctgaat
     5881 gaaagataca acgatcttct ctacagtttt ggcgttacct tctcgttcgg cggagccgaa
     5941 aaaggcaaaa agctgcttga tatcaaattc gatttcgaca aaacagatat aaaggcggct
     6001 tatcataaag acattaatgc cgtgacaaaa ttgatgcaga aatatcccga cctcaatatc
     6061 gtgaccgagg gacatacgga taacatcggc acgcctgaat acaacaaaaa cctttccaat
     6121 aaacgtgccg atgccgttaa aaaatacatg gtcgaaaaag gcggtgttgc tgccggacgg
     6181 attgatccca aggggctggg agaaggatcc cccgccgtat ccaatgatac agaggcagga
     6241 cgcgctgaaa atcgtcgcgt ggaagcggcc gtggactaca ccggcaaaaa gtagatcaat
     6301 ggcttgaacc attatcaaaa agccccttca aatctttgga ggggctttta ttttaggctg
     6361 gagggggaag gcagctccgg aggacatcct gcgaaaaagg gagcgctgtc atcctttttt
     6421 ccggtctgtc agtctttttt tactgactct ctttgatcag cattgttgcg ggatcgagca
     6481 gtaaatcgtt ttgggctgaa tttttcggtt ggcctttgac agacgtgata tacacctcat
     6541 ccccttttgt cgcaagctga acaatgactt caaagagatc cgcgatatgc agaaaggaaa
     6601 gcggcaggcc gttttcctgc ggcggttcat gacgatgggt atgcacggta aaccataagc
     6661 ccatggagta tttaccggcc agttccttca actgcaccag ctgaccgcgc cccgcctcgt
     6721 cgaatctgaa tccgtcgata atgacggtgt gcggcttgaa aatattctgt tccatcaggt
     6781 cggtcagacg ctcttccagt ttcggcgcgc tgaatccttc caccttgaac gtcatgatga
     6841 aacggtaggg ctgaatttta tcccagtatt cattcacctc ggcaatgccg aatttctcgg
     6901 caatgtcatg aaaaatttcc tgataccaca ggtcgacctt gctgacggca tcattcaggc
     6961 tgacgtgcag aaccgaatgt tcccgcagca tcatattcaa ggccaattgc acgagcaatg
     7021 ccgttttgcc gacgccggca tatgccagca cggccccaaa accgcccgtg gacagaatat
     7081 cctcattgtc ataaccgagt ttgacaagag gattccggta aatcatttct tttttgatca
     7141 tcacaatgtc cttatacgtt aaatgaataa tatttttttc ccttttcagg gcaacgctga
     7201 ttgtctgcat gttcggccga ttactatgcg gctgtttttt tgttctgagc cgcttccagg
     7261 gcaatttttt ccgcaatcga ctgcggaacc tgccggtaaa gagtaaattc catcgtaaat
     7321 tgggctttcc cttgtgttgc cgaccgcaaa accgtcgaaa atccaaacat ttccgccaaa
     7381 ggcacctgtg cttcaatcac gcacatcaca tcttcatcct gagaaccgat gatcatgccg
     7441 cggcgctgat tcaaaagccc catgaccgat ccctgaaatt ccgccggcgt ttccacaacg
     7501 actttcatga tcggctcatg aataaccggt ttcgcccgga gatatgcttc cttgaaagca
     7561 ccgcgcgccg ccgcctggaa cgccatatcc gaagaatcca ccgcgtggga agcgccgtca
     7621 ttgatgacca ccttcacgcc ggtcaccgga aattccagtt tcggcccctt ggccatgctc
     7681 tgtttaaacc ctttttcaca ggcagcaata tattgggaag gaatcgcgcc gccgaagatt
     7741 ttattttcaa aaacaaaatc cgtatcggca atcggttcca gatagccggc tacccgtccg
     7801 aactggccgg cgccgccggt ctgtttttta tgcgtataat tgaaatcggc gcgctgcgtg
     7861 atggtttcac ggtacgccac acggggattg cccgtggaaa catcagcgcc gtattcgcgc
     7921 cgcattcttt ccacgtaaat atccaggtgc agttcgccca tgccctcaat aatcgtgtcg
     7981 ccggtttcat gatcgacata cgttttgaat gtcggatctt ccttggcaaa tcggttcagg
     8041 gccttggcca tcgccacctg cgatttgttg tctttgggaa caatcgccag agaaatgacc
     8101 ggtttgggaa catacatcga aatcatggtt aaattgacat ccggcgatgt aaaggtatcg
     8161 ccggaagaac attcgatgcc gaatagtgcg ccgatataac ccgctcttaa agattcgaca
     8221 tcttccatct gatcagcatg catgcgcacc agacgtccta ccttcacctt tttgccggtg
     8281 cggacattca cgatggtcga cccttttgcc aacgttccct gataaacccg gatatacgtc
     8341 aactggccgt attgaccgtc ttccagtttg aagaccagag cgacaatcgg tttttccgta
     8401 tcattgttca ggacgaccgt ttcttcgttt ttatccatat ctatcgctac gttttctatg
     8461 tccgacggac agggcagaag ataggtaacg ccatcgagca tcggctgcac gcctttgttc
     8521 ttgtaggccg atcccaggta aaccggtgtg atctctcttt gcagcgtccc cttgcgtaaa
     8581 gcggaataga tcaagtcggc gctgacttct ttttcttcaa gaatcgcttc cgtcaattcg
     8641 tcggaaaaca tcgaagccgc atcaatgagt tcttctcgtt tcgctttggc ctcttccaga
     8701 agatgagacg gaatttccgc ctcgcgaacc gtttccccgt tatcgccgtc gaaataaagc
     8761 gctttcatac tgaccagatc aacgacgccc tggacatcat tttccaggcc gatgggaatc
     8821 tgcatcgcca cggcattgtg tcccagcttc gatttcaact ggctcatgac acggaaggga
     8881 ttggcgccgc tgcggtcgca tttattaatg aacgcaatac agggaaccct gtaccgtttc
     8941 atctgacgat caacagtaat ggactgcgac tgaacgcctc ccacagcgca caaaaccaga
     9001 atagcgccgt ccagaacgcg cagagaccgc tccacctcga tggtgaaatc gacgtgaccc
     9061 ggcgtatcga tgatattgac ttcgtggcct ttccattcac aatacgttgc tgccgaagca
     9121 atggtaatgc cgcgctcctt ttcaaggtcc atggaatcca ttgtggcgcc gacgccgtct
     9181 ttgcctttga catcgtgaat ggcatggatt cttttcgtgt aataaaggat tctctcggtc
     9241 agcgtcgtct tgcccgaatc gatatgggcg ctgatgccga tattacgaat tttctgatcg
     9301 tcaattttca ttagtttttt ccttctttat cactgttata attccattcg cttcgctcag
     9361 ggattgttcg acttacacgt ttcagtgcat ttcatttctg aaacgtgagt ctcacaaaaa
     9421 aaccccacac ttgtaagtat ctctgcaaga tggggcatcc accataaaca agcatttgtg
     9481 aacttggctt catacatatt gcaacgcaat atgtcaagca gttatttaac atatttagaa
     9541 caataagaat gcttgtgtat tattgttgaa catgataatt atctttcatg tttaattaat
     9601 tgaattataa aggagttttt gatgtacgag cagatattgc caaaccttta tcggattatc
     9661 atacccctgc ccaatagccc gcttaaagat ctgaattctt acgtcattaa agccgataat
     9721 cggaatctga ttatcgatac cggattcaac cgcggtgtct gttatgaggc catggaaaat
     9781 ggcctgcacg ccttggagat tgatttatcc agaacggatt tcatgctcac ccatatgcac
     9841 tccgaccaca cgggactggt gtcccggctg tttacggaga ccagcaaagt ctatttcagc
     9901 aggatcgatt ccgaagtatt caacgaaaat atcgactggc aggccatgct ggattacgca
     9961 ttgcataacg gtttcgaact ggaggaactc atccgggccg cagagagtca tccgggattt
    10021 aaatacagac cgaggaaaat tcccgacttc accatgctgg acgacggcag cnnnnnnnnn
    10081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn natttaaata cagaccgagg aaaattcccg
    10201 acttcaccat gctggacgac ggcagcatga ttgaaatcgg cgattataaa ctccaatgca
    10261 ttcttacacc cggccatacc caaggtcata tctgcctcta cgaagaagac aagcaaattc
    10321 tattttccgg agatcatatt ctttacgata ttactccgca catcgagtct tggtcatacg
    10381 aagtgaattc tctggaaaac tatatgcaga gcctcgacaa ggtgagaaat cttccggtca
    10441 aacttgttct gcccggacac cgcaacttct ttaccgatct caagggacga atagacgaat
    10501 tgaagaacca tcacgaaatg aggactaaag aagtacttga cgttttgggt aaaagtacca
    10561 tgagtgccta tgatattgct gcaggcatga cctgggatat cgattgcgaa agctgggaaa
    10621 agtttccggt ggcccagaaa tggtttgccg tcggggaagc cattgcgcac ctgctctacc
    10681 tggaaagcaa aggcaaagta aaaagacatc cgggtgaaaa aacagatttt ttttcagcaa
    10741 tccggatgga atgatttttt gtccggctta ttgtattttc cggcgcaggc cgctcagagc
    10801 aaaccgccct tcctgtggtt ttttctgttg attttttctg cattctgaat gttttcggct
    10861 gctaatacga ttgccccagt attttcataa tggtttcgat cagctttttg gtcctgacgg
    10921 gtttcgccat gatgtaatta tctcccattt tattgctttc gccttcaaca atttcgtcat
    10981 tggacgcaag agccgtaata tagagaatcg ggatatcggc cgtggcagga tggtctttca
    11041 attcagcggc aacatcttca cccggaatgt ctggcataaa caaatccagc agaatgatgt
    11101 ccggcttttc ttttctggca agttttattc catctgcgcc gttgtttgcc acaaccacat
    11161 cgaacaggcc ggactgcgta agattttttt tgatgaaata acaaaaatcg acttcatcat
    11221 caatagccag aactttgatc tgatttgtca tattgtccct cctcggcaac tacctttttt
    11281 atctgcggct gaatattgac ataaaatgtc gttcccttat tctcctcact ggtaacatca
    11341 atcgttccac tcaaacgctc gatgatcatg tgaacgatac taagccccag accggttcct
    11401 tcattgacgg atttcgtggt aaaaaacggt tcgaatattt tcgactgaac atctttggac
    11461 attccctttc cggtgtccga tatctctaca acaatctttt tctcgccgat tctgaaaaaa
    11521 tccgtatagc gataaccggt tttgtaaccg atttccgata tgattttccc ggaggcgcga
    11581 atggtcaatc tcttttctgt actctccttc atcgcgtcaa tggcgttgtt gataagattc
    11641 agaaaaacct gctccagcat gttataatcg cccgccgcct ccagcgattc ggtttcatac
    11701 tcgcgcttca cggtgacatg tcccttgtga agggcctgat tggccagcac cagcgtgttg
    11761 tcgagcaatt ggcagaccgg aatctgcgta attttgtatt ccgattttct ggaaaaagac
    11821 agaatgttgt ttatgatatt gtcggcgcgg tttgccgctt tatatatttt ttcgatggag
    11881 ccgtcgatct gagggtcttg gtgggcaata ttgttctcca gatagtcgat ccccatgtaa
    11941 ataatttcaa gaggattcct gatttcatgg gcaatgcctg acgccatgcg ccccagagcg
    12001 gccattttat cctgttggat cagctggtcc cgcgttttga tcaacagctc aaccgtatta
    12061 ttcaattttt tttctttttc aatccggtcg gtgatatctt tcgagataac gcagacatcc
    12121 agaacatcac cggttgacgg atccctgatc ggaccgagcg tattggcaaa ccacaaatta
    12181 tgtttcttat tataggattc gtatcgcacc atgtcgtttt tttgatacac cgactgcacg
    12241 taatccagaa gtttatccgc ttcggatgca tcctcggcat tcacctgcgc aatggtcagt
    12301 ccggcaagac gcttgtcttc atgataaccg taaaaccggg caaatatatc attggcaaat
    12361 tttaactttc cgtcgcgatc cacaatactc atcaggacat cggtggcttg aacaacctgt
    12421 tcatacctct gccggacttc atttatccgc atgtccttgt cttttaatcg cgtcgtgatg
    12481 agatagccgt aaaaaattgc gatgacgatg atgaatggaa tctggatcag ataagcgccg
    12541 gctttcggac cggtcaacac ccccttctca tgcagcagcc agagatagat gagacaaaat
    12601 aaaaatgtat tgatcatcag atacccgaaa cgcgagctca tggtggtcaa tgaaataata
    12661 aagaaaaaca tcagatataa gtcggatccg atatatccgg aaaaataaat tccggccgga
    12721 agaagaatac tatcgcataa gataaaaccg taaaaaattc tgttatcgtg aaaatatttt
    12781 tccgggatat acgcgacaat cagattggtc atcaggtaaa cggcgatgaa gatgtacccg
    12841 taaatttcca gttctttgcc cgctaccggt gacagcagaa taaaataggc cgtggtaatc
    12901 acaaccatca gccggataaa aaatatcgtt cttttccttg gaatctgtgt cgccatgagc
    12961 tacccttggg attcaggcgt tgcttgatgt ttaaaaataa tacaggacaa acattttttc
    13021 atcaagaaaa ttatgatttt aaaactatga tttaacgatg atgtttcata cggatcagca
    13081 tcctgacgcc accggttaat cccggcggct ttgatcatca ccgtttctga tccagccacg
    13141 caatgacatc actaaaaact tctttacggt ttatttcgtt aagcatctcg tggcggccgt
    13201 cggcgtagaa ttttaaagtc acatcgtcaa tgcccgcgct tctcagtgca ttataaatct
    13261 gaattacgcc tttggtattc gctcccacgg gatccttcga accggaaaaa agatagaccg
    13321 gcagatgctt cggaattttt gcgatattat cctttttgtt gatataggaa aggccttcca
    13381 gcatatcgca aaaaaatccg gccgtaaaaa ctccgccgca ccatggatcg ttaatgtatt
    13441 tgtccacctc ggcatggtcc cgggaaagcc agtcgtattc cgtgcggttg ggtttaaaag
    13501 catgattgaa agcgccaaac gacaatttgt tcataatttc acttttcgct tttttgccgt
    13561 gaagcctggc atccatcctg gcaatgaaaa gccccgcccg acctgccacg ccgggatcac
    13621 cgcccgttcc cgacagaata aggccgttga gttcccgtcc gtaaagcatg gcataatgcc
    13681 gggaaagaaa agatcccatg ctgtgaccga agagaaagaa cggcttttgc ggatagtcct
    13741 ttttaataag gccggtcagc aggtgcatgt cctctacaac ttttgcccag ccgttttcgt
    13801 cggcaaaata gcctctatta tccggcgatc cggcggttct gccgtgcccc cggtgatcat
    13861 tggcgtaaac ggcatatccc gcttcggtca gggcgccgcc aaaacgttca taacgtgcgg
    13921 catgctcagc catgccgtgt gctatctgca cagccccctt cacggcatcc gcgccgtcag
    13981 gcgtccactt gtaaacgaac atctgcatcc cgtcggttgt tttcaaaaca agggtctctt
    14041 ttttcatttt tcctctcctg cacatcatta ttcaaggtta atatccgccc acacgattcc
    14101 tgacaaaagc ggcaatgtcc tccgtggcca ttattgcctc gggaagactg accaccgaat
    14161 gaaaaacatg aaacagatcc tcccatacgc ggagcgtgca gtccacgcct gaattttcga
    14221 gggaagcggc aatacggagc gagtcactca ggagcatttc gcaggttcct acatgaataa
    14281 gcaccggcgg cagccctctc agatccgcgt atagaggaga aataaacggc aagcgcacat
    14341 cggcatgtcc cgcatacagg cccgccatgt aatcaagacc ggatcgcgtg atcatgggat
    14401 ccctggcggc atttatttca atggactcgc cggtcacggc cagatccacc cacggcgaaa
    14461 cggcaaatac cgcagccggg agagcttctc cccgttcttt caacttcagc atcgtcgcaa
    14521 acgtcagtcc ccctccggcg gaatccccgc cgacaacaat cgattccgca gacacaccct
    14581 gccgcaaaag ccagcaatat gccgagagcg aatcttcaat cgcggcagga tagggatttt
    14641 ccggagcgag acggtagtca acggataata cgctcaggcg ggataactcg ctcaacagcc
    14701 ccgtgaatcc cgcgtgggtc tgcggagagc ccatggcata accgcctccg tgcaaatata
    14761 aaataaccgc cgtatcgtcc gccgatacag cctttaccca taatgcctcc ggtcctccgg
    14821 cattgaccgg ttcgaatctg catccggccg gcggcaaaga caaggacatc atgtccgcca
    14881 gaacctgccg acagagctct atcggcaact cgccggcgtc gggcagtttc atcatcgaca
    14941 tcagattcat gacgtttttt gcttcctgac tgatcatggc acatcctccc ggaccgatgt
    15001 ccgtatcatg aaaaaaaata taacagacgt atcgcctttt tcaatgtcat acgctatggt
    15061 ttatctgtgg tttgttatcc gccaatctca accgatggac agaacctttt cccctccgtc
    15121 agccatcgtt tccgatacca tgaatctcta tttggaatac agccagcggc ttaaaacgct
    15181 taaagtattt tggatactga tcggtttggc gatataatcg ttcattccag cgttcaggca
    15241 tttttccttg tcgccactca tggcctgagc cgtcatggca atgatgggca gcgatttgaa
    15301 tttcttattc cgccggatta ttttcgtcgc ctggtatcca tccatttcgg gcatctggac
    15361 atccataagc accaggtcaa attgctcttg tctgatttta cccagggcgt cctttccgtt
    15421 ttcggcaacg actactttca gccccgcctg ctgaagaatt tcacgggcaa cctgctgatt
    15481 gatgaagttg tcctccacga gcaatatccg ctggccggca agcgcctccg tcgcaggagc
    15541 cttgaccgga ttacggaccg cgggggagac ttttttgatc gtttggccga agatgttcat
    15601 cattgcatcc aaaagattcg accggtgaaa tggttttttg aggaaatatt tcacgcccag
    15661 tttttcagca acggttttgg tatcatcctg aaaggatgtt gaaacgatca tcaccgcagg
    15721 aatcgcggcc aacgcggcat tttttcttat catcctgacg atttttatgc cgtcctgggc
    15781 cgacgttcct gattcgacca acaggagatg aaagttgtct ttctctttca gaattttcaa
    15841 ggctttcgat cccgacgcgg cggtggtaac actcaagccg ataccttcca gataatccgc
    15901 gagaattttt tgagccgtcg gatttttatc aacgatcagc acccttttat ttttaaaggg
    15961 tgtgccggaa aagctcgttt tgcctccttt tttctttttt cccggctcca ggggaatcat
    16021 gaaagaaaaa acactgcctt ttcccgcttc gcttgtcgcc tctatggttc ctcccatgag
    16081 ctgaacgatc tgtctggaaa tggccaatcc gagaccggtc ccgccgtagc gtctcgtgac
    16141 ggaactgtcc gcctgcgtga acagttcgaa caaatcgccc atttgttctt ttgtcagacc
    16201 gatccccgta tctctgacgg aaaaattcaa atggatataa tcctttcgtc ctcttttgaa
    16261 tctggcgcac gcttccacgc ggacaaccac ttcgcctttc tccgtaaact tgacggcatt
    16321 gccgatcaga ttcatcagca cctgttgcaa acgccaggga tcacccttca aaaagagagg
    16381 gacatccctg tcgatgtgga agagcagatc aagccttttt tgcacggcat tcggatacat
    16441 gatatcggaa atatgcgtca tgacatcttc aagattgaaa tcgagaattt ccagtttcat
    16501 tttgtccgct tcgagtttgg aataatcgag aatatcattg atgatcacca tcagtccctg
    16561 tgccgaagat tctattttat tcagatagtc tttctgtttt tccgtcaggc cggtttgaag
    16621 ggcaagattc accagaccga tgatgccgtt catcggtgtc cggatttcat ggctcatgtt
    16681 ggccaggaaa gcgcttttgg ctcttgtcgc ttcctcggca atttccctgc ctctgaggct
    16741 ttcctcaagc gatttttcct tggctttgat ctcggtgaca tcccggatca tctcgatgga
    16801 accgaagcgg tttccgtcct tgtcgaaaag cggagccgcc acaccccaca gatgcgctcc
    16861 ctggccgtca cgcagtccgg aaatatatga ttcggcgtaa atcttgctgc cgcgttccac
    16921 atatttgtat aacgcttcca tttttttgtc tcttttatcc agaagatcga tcagcatggg
    16981 ccgccgccgg ccgtaaaagg gtatcgcata ttcataatgc cccttgccca gtatggcgtc
    17041 ctttgtaata cccgtcattt cttcgatggc gcggttccag gcaatcactc tgccgtcgcg
    17101 gtcaatgacc aatgttgcgt caggaatgaa ttcgatgata tcgttcaacc gccgggtcaa
    17161 cgaaagcaac tcttcttccg ctttcttgcg ggcggtaatg tcccgggcga ttccctgaat
    17221 ggccaccggt tctccgttgg acatgatggt tgctccctga gtttccacat aaacagcgct
    17281 tccgtctcta tgacgcaacc gaagttcctt tagaccctgc tgtaccccgt attgatggat
    17341 ttcttcgata atgtccagcg ccacggggat ctgatcgtca tccagaaggt tcacgatgga
    17401 caacgacgat atttcatctc ttgtataacc gaccagatca agggcggcgg aatttgcatc
    17461 aaggaactgc ccgtcgaaac cgacgacgaa aacaagatcg atcgattgct caaaaagggc
    17521 gttatatctc tcctcagttt cttttaattt attctccgcc tgtttgcgct cggtgatgtc
    17581 gcgggaaatg ccgccgataa cgacatcgcc gttttccagc agaacgggaa atttggttga
    17641 ttcgtatatt ttatcgtgca gcctttcttc tacgacaacc gtcgattttg tttgcacgac
    17701 cctggtgtcg ttttcccgaa agtagtcggc cgtttccggc ggcatcagct ggaaatcgtc
    17761 ctttccgatg atgtcttgca gattcatacc cagaagttct tccatggcct ggtttaccag
    17821 gatgtagcgg aatcggctgt ctttaagata aaccatggca gaagtggcat tgataaatgt
    17881 gcgatagcgt tcttcgcttt cccgaagggc aatttccatg cgctcctgat cgctaacgtc
    17941 gcgcagaatc atgcttgttt tgaatttccc tgcgctgtcc tgaaaaacgg atgtgctcac
    18001 atccgccgta aagcgtgttc cgtctttccg gaaatgaaac agccggccgc tgaatttacc
    18061 ggttcgggct ctctccgcca gcaaaacctc tagacgggga tcggatgtat ccaccagtcc
    18121 tgtacgtccg gattccagga tctcctgctt tgtccggccg aacatcctgc aggccgccgg
    18181 gttggcatca ataatcgaac catccggtcg ggtcagcaaa atggcgtcca tcgagtgatc
    18241 aaacagcgag tgatatttca cttcattttc ccggatggct tcctcggcct gtttgcgttc
    18301 ggtgatatcc ctcccgacgg caagaaccag tttttcgccg ccgatatcgg ttttcatcaa
    18361 ctgcatttcc atccagaaat gtgtcccgtc tttcctgcgg gccagccatt caaattgttg
    18421 cggcccttcc tgaatcgctt ttttgataag tctcagcgct ttttcttccg tatccggttc
    18481 cggattgccg cttccatcag cgaaactaca cctgagtgct tcttcctttg aattgtagcc
    18541 gtaaattctg atcattgaat cattgacatc gataattttt ccggtttctg catcgtgaat
    18601 aaaaatggct tccgatgtgg cgttgaaaat ttcccggtat ttttcctcgc tcgcctgcag
    18661 catttcctcg gcctgtttgc gttcggtaat atcccggatc acacctttga aaccgacgat
    18721 attgccggat gaatccttgc gcagcataat cgaagcttct atggatatcc gggagccgtc
    18781 ttttcttttt atcggccagt catagccttc tgacggccgt tctgttttat atactcggtt
    18841 gaacgtttga taaactttcc gggtggtttc gtcatttgcc gaaaggcggc tgtaattcat
    18901 ccccctcaat tcgtcccggg aaaaccccga catccggcat accgaatcgt tgaagaaggt
    18961 aaaattgccg gccaggtcca cttcaaaata ggcttcgcga atggtttcca gaatattgcg
    19021 gtatttttcc tcgctccggc gaagctcttc ctccgctttc ttgcgctcgg ttctatcccg
    19081 cacgattccg cggaaggcaa taacctgacc tgctgaatct ttaatggccg atacggaagt
    19141 gtctacatac tttttttgcc tctcttttgt gaatatttcg tattcaaaat tattttccga
    19201 cccacctgtt ttgaagattt tgttgtatac atcgaatact ttccgggcgg tctcttcatc
    19261 catcatttcc cggtagcgca ttttcaacaa ttcttctttc ggatatccgt ctatccggca
    19321 gagcgcatca ttgacaaaga tgaaatcgcc cttcagatta agctcggcgt atccgtcttc
    19381 aatattctcg atgatggccc ggtaccgttc ctcgctctct ctcagcttgt cttcagcaag
    19441 tttccggtcg ctgacatccc ggcagacaaa cacgaacccg aagggattgc cctttttgtc
    19501 ccgaatgaca tcgccattca cctcaaaggg aattatcctt ccgtccttca taacgatttt
    19561 gtattcatcg ggacccagct tgcgatcaag catcagtgct gcgtttttca tcagtctttc
    19621 atggtcgtcg ggagccgcaa acaaaaccat gttccggcct tcaatttcct cccgttgata
    19681 accgcttatc cggagagcgc ggtcattgac gaacagcacg gtgccttcca ggctggttcg
    19741 aataacaacg tccggcatgg tatcaaccag tcttgcatat aactccctgc tgttgcgcaa
    19801 cgattcaagc gcggcatctt tctgcaactc cgccacccgc tgccggtctc ttatttttgc
    19861 ctccgccagt tcccgggtga ttgccggaca aagccgggac aggttgtttt tcatcaaata
    19921 atcatgggct cccatgcgca tgcactcggc ggcggtttcc tcgccgatgg ctccggatgt
    19981 gacgatgatg gggatattga gttttgtctg ctttaacaga gcgatggccg aaggcgcact
    20041 gaatttgggc attttataat cgcaaatgat gatgtcccat tgcttttcct cgagggcctg
    20101 cgtcatggcg gcggcggttt cgatgcgttc atagatcggg gcaaatccgc cttttctcat
    20161 atgccgtatg atcagcaaca catcatcttc cgaatcatcg atcatcaatg cccgcagttt
    20221 ctgcggaccg ctgaagtccg attccagccc gattggttgc gatatgtttt ttcccttcat
    20281 gacttatcct ctcctgtgac gtggtttacc ctcgtccatg ggtaagattt ttttgcaaac
    20341 cgatttgaat cattgatttg ccggcgtgtt ccgaaacgtt tatggccatt tttttctcgc
    20401 ggatgaaatg aacaaaaaag atatggaaaa aacaggaaag ggaataaaaa tgatttgatg
    20461 tgtccggtac cgtatgatat cccataatgt ctccggtgaa aaaaaccgtc agacatgaat
    20521 accctttcta ttcattcccg gttatgaaat ttctgttacg tgtcatgttt taaacaaaaa
    20581 gcaaggccaa ccgaatccga tccggcaaaa aggatcatat atacttgcgt atttcgttca
    20641 ataaagcctt caaatcgtaa ggtttggcca tgaaaaaatg atggccgatc atatttttac
    20701 gggtttcttc ttcttttttg gtcaataaag ccgtaaggaa aattatgggg atattcctcg
    20761 tcagaggatc gcttcccaga atatttccga catctcctcc gctgatgccc ggcatgttga
    20821 tatcaagcag caaaagagcc ggtttttctt ttttagccag ggatatggct tctttcccat
    20881 tctgagcggt gatgacgaga taaccttctt gcgtcaaatc attttcaacc agtttgagaa
    20941 acactttgtc gtcatctacc acaagaatct tcttactgtt gtccacctga cacctcctcc
    21001 ttgactttct caaaatcagt tctcaatttt ccaatatggc ttttgataac agaaatttcc
    21061 cgtttctcca ttgcctccaa ttctatttct ttggccagct tctgaattcc gcttattcgc
    21121 aggtttcccg ccgacccgct gatcaagtgc gcttgcttgc gcatttcctc ataattttta
    21181 cgggtgacgg tttttttcag tatttttaaa gcggtttcgg tctgctcaac aaatacgttt
    21241 aacaattcat taaacatttc ttcggaaatg tttatttccc gtagagctct tttcttttct
    21301 atcagcaaga aattcctccg gagaagattt ttcaaccaat atgagacgac ggagttaccg
    21361 ggatgatttc ccgttgatgc atctatcgct gtattataaa ttatggcgta taatacaata
    21421 aaattagcgc agtgtatagt gtttctttgt ttcttttatt tgccggaaga ttaaatggaa
    21481 tctttcaccg aacagcgggt aaagaaaaat ccgttaatca acaaaaatat tttacctgca
    21541 ggcattctat tttccggacg atgtcaaaaa ctggacggcc ttttccagac catcgagcgt
    21601 gagttcgtac atggtaaaaa cctgtcggat cgccttgact gtcgtgctgt aataccattc
    21661 gtcaggaggc atgggattca gccacacgct gtgtggaaag gttcgtgcga tgaagcgcag
    21721 ccgctcaata ctggttcctg acggtttggc gtcaacataa atggcgccgt tgggatgcat
    21781 gagttcgtac ggag
//
LOCUS       DAOOLB010000033        20130 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_2653_length_20130_cov_39.764284, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000033 DAOOLB010000000
VERSION     DAOOLB010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 20130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20130
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2653_length_20130_cov_39.764284"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PLF77_05800"
     CDS             complement(<1..1253)
                     /locus_tag="PLF77_05800"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPC08405.1"
                     /translation="MKKSSLSYLGFILTVLFLLTFASYSSAEPIYATKEPVEIYDPLY
                     VGVFGGFIVPDDLRVDGGPDFQLDNSWAAGAKAGYIFPFKWLAAELEYAHLAKHDIDE
                     PGTGHFKADNLMGNLLLRYPLGMIRPYLGGGIGWSFAELESGLTDDTDNNFGWQALAG
                     INLEIIPSLSVDFGYRYFTSDYGINNLDATIHDHILSLGFNYHFGGKKPEPPPPPPEP
                     VKKCPNPPSCCILDHEGCPVDSDKDGVCDGCDKCPDTPECCIVDENGCPKDSDKDGVC
                     DGCDKCPDTPSCCVVDENGCPKDSDKDGVCDGCDKCPDTPAGVKVDQDGCPIPEIVEK
                     GRTTLEVLFDFDKSAIKGNYSKDIDALADVMKKYEDLNVTIEGHTDNLNRSGNPNYNK
                     NLSRRRADAVKKYLVEKSGIDAKRLT"
     gene            complement(1449..1525)
                     /locus_tag="PLF77_05805"
     tRNA            complement(1449..1525)
                     /locus_tag="PLF77_05805"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1489..1491),aa:Asp,seq:gtc)
     gene            complement(1563..1638)
                     /locus_tag="PLF77_05810"
     tRNA            complement(1563..1638)
                     /locus_tag="PLF77_05810"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1603..1605),aa:Val,seq:tac)
     gene            complement(1717..4122)
                     /gene="lon"
                     /locus_tag="PLF77_05815"
     CDS             complement(1717..4122)
                     /gene="lon"
                     /locus_tag="PLF77_05815"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HPC08406.1"
                     /translation="MFDEKIENEQEKTAIIPLLPLRDVVMFPHMIVPLFVGREKSIAA
                     LESAMKYEKSIFMVAQKNAKDDDPKKEDIYHVGTIGIIIQLLRLPDGTVKVLVEGKTR
                     GVIKEYLPNSDFFLVKVNEVDDIDDGNDVKKQALMRSIKESFELYLKLSKKIHVEMLG
                     TVVAIEDASKLADVVITHLNVKLEDKQKIIEIFDISERLEAIYSLMLSEIEILQVEEK
                     IKRRVKKQMEKTQKDYYLNEQMRAIQKEMGEKDDFRNEINELEKRLKQKKMSEEAVKK
                     VKQELKKLQMMTPMSAEATVVRNYIDWILDMPWSEKTENKYTLKESEEILDADHFGLK
                     NVKERIIEYLAVQSLVKKNKGSILCLVGPPGVGKTSIAKSVARATNRKFVRISLGGVH
                     DEAEIRGHRRTYIGAMPGKIIQLLKKAGSNNPVFCLDEVDKLSSDFRGDPSAALLEVL
                     DPEQNFAFNDNYLDLDYDLSEIMFITTANVLQTIPAPLQDRMEVIRIAGYTEPEKLQI
                     AKKYLIAKQVEANGLVMENIEFTHGALLKIIREYTREAGVRNLDREIASICRKVAKEI
                     VNNDKKGRVVIGSKSVGKYLGVPKFHHGEIEEKNKIGLTVGLAWTEVGGELLSIEASI
                     MEGNGKMIMTGKLGDVMQESVQAALSYIRSRADEFGLTKNFYRKIDIHVHVPEGAIPK
                     DGPSAGVAMTTSIASVLLRKKVRADLAMTGEITLRGRILPVGGIKEKVLAAHRGNIKT
                     VIIPKDNQRDLAEVPQNVQKALKIIFAEHIDDVLKLAFVQDEGEMRPAVDANVPMRSQ
                     SVN"
     gene            complement(4141..5403)
                     /gene="clpX"
                     /locus_tag="PLF77_05820"
     CDS             complement(4141..5403)
                     /gene="clpX"
                     /locus_tag="PLF77_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPC08407.1"
                     /translation="MIKKGKNNRMDDGMLFCSFCGKMQSEVRKLVAGPTAYICDECIE
                     LCRYIVEEEDDKLIEKDAQKALPYPKEIKKFLDQYVIGQEKTKKILSVAVYNHYKRLF
                     SRVESDGIEIQKSNVLLIGPTGSGKTLLAKTLAKFLNVPFTIADATTLTEAGYVGEDV
                     ENIILSLLQNADYDVARASKGIVYIDEIDKIAKKSGNPSITRDVSGEGVQQALLKIME
                     GTMANIPPKGGRKHPQQEFIQVDTTNILFICGGAFNDLESIISKRLGVNAMGFGAEIK
                     SKKEKRIGELLSEVRSEDLLKFGLIPEFIGRLPVIATLDELDEAALIKILVEPKDAII
                     KQYKKLFALENVVLKFTDGALKAVASLSTERKSGARGLRSILENTMLEVMYDIPSQNN
                     IKECVINEDVVLKKEKPILIYESKSESA"
     gene            complement(5404..6003)
                     /locus_tag="PLF77_05825"
     CDS             complement(5404..6003)
                     /locus_tag="PLF77_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HPC08408.1"
                     /translation="MVVEQDGRGERAYDIYSRLLKDRIVFLGTAIDDNVANLIVAQML
                     YLEAENPDKEIFFYLNSPGGIVSAGMAIYDTMRYIKSPISTVCMGQAASMAALLLAAG
                     ERKKRFALPHSRILIHQPLGGFQGQATDIDIQAKEILRLKDELNKILADLTQQPLAKI
                     VNDTERDYFMSSEQAKEYGLIDEIIIRKPDASAVRAKEK"
     gene            complement(6015..7340)
                     /gene="tig"
                     /locus_tag="PLF77_05830"
     CDS             complement(6015..7340)
                     /gene="tig"
                     /locus_tag="PLF77_05830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPC08409.1"
                     /translation="MSQVVINVEDLSPVKKKVSLEIPWDDVKNELDAVYQEVGKKAKI
                     KGFRPGKIPRKILETYFKDQAEEETITNIVNKYYWQALEGKGIVPVSRPEINQDGIKD
                     NTNFSFSASFETEQDFEPRHYKGIDVEKEQIKVTDDDVQKRIDEIRQMFATMEEVTDD
                     RPADKGDFVVMDFEGTLNGEKYKELKADDYFLEIGSQKFVPGFEDQLIGMKKGEARDI
                     HVSFPTDYHESRFAGQEVVFHVTVKSIREKKLPENDDSLIKNFEKYESFEDFKNDVRK
                     TLEDKAREMDKVKLQNKITDILVSENELEVPSALVERQIYYMMMETQQKMLSAGIDEN
                     TAMDFNLKMKDKYKPDAEKIVKSFLVLKKIAEKESITATDGDLESYLRELAQHSGRDY
                     ESFQKMYNIEERKDSLMMELVQKKVFDFIEQNANIKVVEKVGLNMEAKS"
     gene            complement(7390..7476)
                     /locus_tag="PLF77_05835"
     tRNA            complement(7390..7476)
                     /locus_tag="PLF77_05835"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7440..7442),aa:Leu,seq:tag)
     gene            complement(7750..7826)
                     /locus_tag="PLF77_05840"
     tRNA            complement(7750..7826)
                     /locus_tag="PLF77_05840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7790..7792),aa:Pro,seq:ggg)
     gene            8031..8402
                     /locus_tag="PLF77_05845"
     CDS             8031..8402
                     /locus_tag="PLF77_05845"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPC08410.1"
                     /translation="MKLRDVKEILDAHVVVGEEKLDMDVHTAFGADLMSDVMAFAKAG
                     SLLLTGLTNPQVIKTAASRNIAAIIVVRGKEPTTEAIAMAKDLQIPILTTNYILFETA
                     GILYSKGIVGCLKKVESGFSK"
     gene            8643..9017
                     /locus_tag="PLF77_05850"
     CDS             8643..9017
                     /locus_tag="PLF77_05850"
                     /inference="COORDINATES: protein motif:HMM:NF018753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPC08411.1"
                     /translation="MKLREVKDILNADVIVGEDKLDIEVQTAFGADLMSDVLAFAKAG
                     SMLLTGLTNTQVVRIANVLDIAAIILVRGKKLSTEAISLATDLHIPILTTKYILFETA
                     GRLYAKGIVGCLEKVESGSDRI"
     gene            9004..9447
                     /locus_tag="PLF77_05855"
     CDS             9004..9447
                     /locus_tag="PLF77_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08412.1"
                     /translation="MIESDKNQHVFTFDVEGGNFNAAGTVSTRIKSLLKQLQMPDDVV
                     RRSAIVSYEAEINIVSYAKKGVIRITVSPDQVMIEADDTGQGIPDINLAMQQGYSTAN
                     HLIREMGFGAGMGLFNIKSYSDKFDITSEVDKGTFLRIIIKEHVT"
     gene            9458..9799
                     /locus_tag="PLF77_05860"
     CDS             9458..9799
                     /locus_tag="PLF77_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08413.1"
                     /translation="MDIGIIAEKLNLKFMSASDRKVNNVTGGYTGDLLSDVMANSHGG
                     DVWITRQIHQNIVAVATLKEHAGIILVNACEPAKDTLEKAVQENVPILVSDLSAFELT
                     GRIYNLLHSDK"
     gene            9865..10602
                     /locus_tag="PLF77_05865"
     CDS             9865..10602
                     /locus_tag="PLF77_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPC08414.1"
                     /translation="MMKVFNCDLHIHTCLSPCAELDMHPAALVEKTIDAGLDVIAVCD
                     HNASENVPYVIKAAQGTHLKILPGMEITTSEEVHFLALFDSLSDLIALQHFVDLHLPG
                     KNDEDRFGVQAIVNEHGEVEGLSDKLLIGATDVALNDLLTVVHHHHGLAIASHIDRES
                     FSVLGQLGFIDDSIHFDALEISYATGIRQARERYADLKKYAFITSSDAHYLKDIGRAT
                     TKIMINEPTLSELKMAFSRENGRYVVE"
     gene            10804..11352
                     /locus_tag="PLF77_05870"
     CDS             10804..11352
                     /locus_tag="PLF77_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08415.1"
                     /translation="MLELAAHILDIAENSVRAGAKLVNITIIEDDKKNLLSIEINDDG
                     HGMTEEEIVKALDPFYTTKKVRRVGLGLPLLAEAAKRAGGDLRLKSVKGKGTKLKVTF
                     QLDHLDRQPMGNITTTIRNLIAGNSFVDFLYKHRHNDRRFSLDTRTIRKEIDDLPINH
                     PEILKYIRDVIDDGLEDIQSRA"
     gene            11381..11875
                     /locus_tag="PLF77_05875"
     CDS             11381..11875
                     /locus_tag="PLF77_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPC08416.1"
                     /translation="MKSEDNELLKEFTPEQVDQLNKIIKKHKGEPGSLIPVLEEAQVC
                     LEYLPVSVQKKIAKELNLPLSRVYGVVTFYSFFTMTPRGKHTVRVCLGTACYVRGGKA
                     LTETLQKEFGIHEGETTADRMFTLESVRCLGACGLGPVVVVDEDVHGRVKPSKLKEIL
                     AQYK"
     gene            11903..12286
                     /locus_tag="PLF77_05880"
     CDS             11903..12286
                     /locus_tag="PLF77_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPC08417.1"
                     /translation="MAKLTINDLKKIKEKVHKEMSLRDGERRVKVTVHMGTCGIASGA
                     KEVMDTLLQEIETAGANDVVVTTSGCMGLCSREPLVTVEILNQEPIKYEYMNSNKMRQ
                     VFKRHIQGGEIQTPFVLARGAEVIK"
     gene            12352..15408
                     /gene="nuoF"
                     /locus_tag="PLF77_05885"
     CDS             12352..15408
                     /gene="nuoF"
                     /locus_tag="PLF77_05885"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPC08418.1"
                     /translation="MKVLRAHLLICGGTGCQASGSPAVKKALLEELDKKKLTEEIKVV
                     ETGCNGFCALGPIMVVYPEGVIYISLKPEDMAELVEEHLVKGRIVQRLLYREPTTDQI
                     IPTMQDIPFFAHQELRVLKNRGLIDPEKIEEYIARDGYAGMAKALTEMTPEQIVQEIL
                     DSGLRGRGGAGFPTGLKWKFAAQSKGDVKYVLCNADEGDPGAFMDRSVLEADPHAVLE
                     GMVIAAKAIGSKHGYIYCRAEYPLAIHRLNVAIDQAKKAGLLGKDILGTGFDFDLEIY
                     QGAGAFVCGEETALMTSIEGKRGMPRPRPPFPAVAGLWQKPSILNNVETLANIGQIVL
                     RGAKWYASIGTEKSKGTKVFALTGDVANVGLVEVPMGTKLGTIVHDIGGGIPKGKKFK
                     AAQLGGPSGGCIPIQHLNAPVDYEKVAELGAIMGSGGLIVMNEDKCAVDMARFFMDFC
                     QDESCGKCTPCREGTKRMLEILTNITQGKGKEGDIELLEEMAQVIKNAALCGLGQTAP
                     NPVLSTIRYFRNEYEEHIRDHRCRAAVCSAMFKSPCQHTCPIEMDIPAYIALIRAERF
                     DDAYKILLQTNPFPSVCGRVCDHKCQTKCRRGNLDEPVAIKFLKRFITDNAPRPKVDP
                     VLVSRKEKIAVIGAGPAGLTAARDLALRGYKVTVFEELPKPGGMLYWGIPSYRLPRNI
                     LDGEIDDIRALGVDIKLNTRVGKDITFAKLEKEFDYVYLATGAHKSQKMGVKGEDLKN
                     VYGGVEFLRDFNANEEKWLNGKNTLGKKVAVIGGGNSAIDAARVALRLGSEVTILYRR
                     LRQDMPAAEEEIKAAEEEGIKIDFLVAPLNVEGAKGKVTGITCQRMKLGDFDSSGRKK
                     PVPVEGSEFTLKVDAVISAIGQVPDMSFIDAKRGVEINKWDCFAVGKVYKSGTTNPRY
                     FAGGDAVTGPDTAIGAIAAGHQAADDIDAAIRKANGEPAYEKPALEKIDIPLIIDEET
                     VEAPQMAMPELHHETRKTNFVEVELGFKREDAIKEACRCLRCDAAV"
     gene            complement(15519..16439)
                     /locus_tag="PLF77_05890"
     CDS             complement(15519..16439)
                     /locus_tag="PLF77_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08419.1"
                     /translation="MENNRKKKENLGNRQDQNLRHYFETQKELFESWNGNYEKFVTST
                     HNKYKMHYQKEFADFSAFIDYLIKDDEQTVQDTSSSVADDHKKIDSLEAEFDGIFRAW
                     IDQATQERNTDDEDEGKGEWLDYVESQAIATFYSVLINYKEHIENVYHRQLRGKKLGY
                     ARKWMNDDPRVERLIIKYKVCEILNELEKKDSRSAKTKKPFRYLFQLEEPKYQGRYPY
                     ACFITNQDFYNAILQALDIKKITLQKYFKALCQVGAIKKLQGAGRVDIRKNETLYALG
                     YFSGLKGTNYKLNRFLTQKSTNKFLKFKLS"
     gene            16812..17057
                     /locus_tag="PLF77_05895"
     CDS             16812..17057
                     /locus_tag="PLF77_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08420.1"
                     /translation="MPRWTYKTNIIDLDQVIPPDKVQCDLSGDCIVTDLQGGGLPHFK
                     EMLDKEGEGEWELVQCQYHGGKLLCIWKKEIKEAFAS"
     gene            17203..18084
                     /locus_tag="PLF77_05900"
     CDS             17203..18084
                     /locus_tag="PLF77_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HPC08421.1"
                     /translation="MAFNAPAKMVGLVGNAGVYKSKLSVFQFLIRGFMAGAYIAIGGA
                     LCTVCSTGVAPILGPGIGKLIGGAVFPVGLIAIVLTGMELFTGDCMLVPMAAMMKRCT
                     WGSVFRNWFWVYIGNVVGSFFYAYMMAVGPFVDGTADGTLVVNAFGMTAINISVAKIL
                     TYKAVGTAGLWSCFMKAIGCNFLVNIAILLAITADDVISKFFGIWFPIMAFVATGFEH
                     CVANMYFLPTGKFLTDWYPATIEKVGGLLAANGGLSWGDIWFWNIIPVTIGNIVGGFL
                     FIGVAYFLMFRNELPKD"
     gene            18096..18563
                     /locus_tag="PLF77_05905"
     CDS             18096..18563
                     /locus_tag="PLF77_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HPC08422.1"
                     /translation="MSLPAWITFFICAAAVFIHYMQVRNTRFLLFAIPALLALLIIPM
                     LLAWMSRRTLNKAAQENVSKAKLYKIGKVTPAMTGQVVSIKGHVKKISFRWLNRPHFH
                     VEDSTGRIRVIMFTAPDDNIREGDPVDILGIVIKNIFKRGTPAISAVSVKKNE"
     gene            18590..18739
                     /locus_tag="PLF77_05910"
     CDS             18590..18739
                     /locus_tag="PLF77_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08423.1"
                     /translation="MNSREIIFLIGFLILGITPVFAQQLPGGATELIMLAGMLISTST
                     AFVKS"
     gene            19238..>20130
                     /locus_tag="PLF77_05915"
     CDS             19238..>20130
                     /locus_tag="PLF77_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC08424.1"
                     /translation="MANENLESKRWLIAGAAVIIQLCLGTVYAWSVFKNPLMQMHGWE
                     GKSVQYTFMLLMLIIGLAAAFGGTLVDKKGPRFVATIGGILFGIGTLVAGYADQVGNI
                     YLLYLGFGVIAALGNGFGYVTPIATLIRWFPDKRGLVTGLAVMGFGAGAFFMGKIAPV
                     MIKSFQQIDPAGTMIASGVANTWYIWGIIFLILVTGSAQLFKNPPAGWLPKGFTPAAT
                     TVSAADSFTFGEAVKTRQWWMLWAMLCLNVSAGLGLISQHSPLAQDIYKKTYGLTGDL
                     TPEQVAIVAAAGGAVVAYAAI"
BASE COUNT         5161 a         4843 c         4615 g         5511 t
ORIGIN      
        1 gtcaggcgct tggcatcaat acctgatttt tctaccaagt atttctttac agcatcagcg
       61 cgtcttcggg aaagattttt attgtagttc ggattcccgg atctgttaag gttatcggta
      121 tgtccttcaa ttgttacgtt taaatcctca tatttcttca taacatcagc cagagcatca
      181 atatccttgg aatagtttcc cttgatggcg gatttgtcga aatcaaacaa tacttcaagg
      241 gtcgttcttc ccttctccac gatctccggg ataggacaac catcttgatc aaccttcact
      301 ccggcaggcg tatcgggaca cttatcgcaa ccgtcgcaca cgccatcttt gtcggaatcc
      361 ttgggacagc cattctcatc tactacacag cacgacggcg tatcaggaca tttatcgcaa
      421 ccgtcgcaga cgccgtcttt gtcggaatcc ttgggacagc cgttttcatc aacaatacag
      481 cactcgggag tgtcggggca tttatcgcag ccatcgcaga cgccgtcctt atcggaatcg
      541 acgggacaac cttcatgatc aaggatacag catgatggcg gattgggaca tttcttaacg
      601 ggttccggtg gaggaggagg cggttcaggt tttttaccgc caaaatgata attgaaaccg
      661 agtgaaagaa tatgatcgtg tatggtggca tccagattat tgattccgta gtcgctcgta
      721 aaatagcgat atccgaaatc aaccgacaaa ctgggaatga tttccagatt aattccggcc
      781 agtgcctgcc atccgaaatt attatcggta tcatctgtta atcccgactc caattcggca
      841 aacgaccaac cgatcccgcc cccgagatag ggacgaatca ttcccagcgg atacctcaac
      901 agaagattgc ccatgagatt gtctgccttg aaatgtcccg ttcccggttc atcaatatcg
      961 tgttttgcca aatgggcata ttccaactcg gcagccagcc atttgaacgg aaaaatatac
     1021 cctgctttcg ctccggcggc ccagctgttg tccagctgaa aatcgggtcc tccatcaact
     1081 ctcagatcgt cgggcacgat gaagccgcca aatacgccga catataaggg atcataaatt
     1141 tcaaccggtt ccttggtggc gtatatgggt tctgctgacg aatacgatgc aaaagttaaa
     1201 agaaataaaa cggttaagat aaaaccaaga taagacaaag aagatttttt cataagaaat
     1261 cctcctttca cgcaaagggt taatgatact ggcatctact aaacaggata ccttatatat
     1321 aattaaattt taaaagtaaa attatatttt actaaaaaat gatctacgaa tcattacgga
     1381 aagggcatgt ttcccggtca gcaaacaaaa aaaagagcct cgctgacgag actccctttt
     1441 agattttctg gcggggccga tgggacttga acccacgccc tccggcgtga caggccggcg
     1501 ttataaccaa cttaactacg accccaaaaa atcatttcta attatcaatc cttcagcttt
     1561 aatggtaggc gggacaggga tcgaacctgt gacatccacg gtgtaagcgt ggcgctctcc
     1621 cggctgagct atccgcccgc aatgctctca aaacgctgcg aggcttctag caacattgat
     1681 atttcttgtc aagtcataaa agcttaaaaa agctttttaa ttgaccgatt gggaacgcat
     1741 cggaacgttg gcatcgacag ccggtctcat ctctccctcg tcctgaacga aagccagttt
     1801 caaaacatca tcaatatgtt ccgcaaagat aattttcaga gctttttgca cgttctgagg
     1861 gacttccgcc agatcccgct ggttgtcctt cgggataatg accgttttga tgttgccgcg
     1921 atgagccgcc agaacctttt ccttgatacc gccgaccggc agaattctgc cccgcagggt
     1981 gatttccccg gtcatggcca gatctgcacg cacttttttg cgcaacaaaa cggaggcgat
     2041 ggacgtcgtc atggcgacac cggcggaagg accgtctttc ggaatagcac cctccggcac
     2101 atgaacgtga atatcaattt ttctatagaa attcttggtc aaaccgaatt cgtcggcacg
     2161 cgaccggatg tagctcaacg ccgcctgcac ggattcctgc atgacatcgc caagtttgcc
     2221 ggtcataatc atttttccgt ttccttccat gatggaggct tcgatcgaca gaagttctcc
     2281 gccgacctcc gtccaggcca gaccgaccgt cagccctatt ttattctttt cttctatttc
     2341 accgtgatgg aatttgggca cacccagata cttaccgacc gatttggacc cgatgaccac
     2401 ccttcctttt ttatcgttat tgacgatttc cttggctact ttgcggcaga tcgaagcgat
     2461 ttcccgatca agattccgga cgccggcttc gcgtgtatat tcgcgaatga ttttcaacaa
     2521 ggcaccgtgt gtaaattcaa tattctccat caccaggccg ttggcttcca cctgcttggc
     2581 aatcagatat tttttcgcga tctgcagttt ttccggttct gtgtatccgg caatgcggat
     2641 cacttccatt ctgtcctgaa gcggcgcagg aatcgtctgc aaaacattcg ccgtcgtaat
     2701 aaacatgatt tccgacaggt cgtaatccag atccagatag ttgtcgttga acgcaaagtt
     2761 ctgctccgga tcgagcacct ccagaagcgc agccgacgga tcgcctctga aatcggagct
     2821 gagtttatcc acctcatcca gacaaaagac cggattattg gaaccggctt ttttcaaaag
     2881 ctggatgatt tttccgggca tcgctccgat gtaggtccgg cggtgtcctc tgatctcggc
     2941 ctcatcgtga accccaccca gggaaatacg gacaaatttt ctgttcgtcg ccctggccac
     3001 cgatttggca atggatgttt ttcccacacc cggaggaccg acgagacata aaatagaccc
     3061 cttgtttttc ttgaccaggg attgaacggc aaggtattcg atgatgcgct ccttaacatt
     3121 cttcaatccg aaatgatcgg catccagaat ttcttcggat tctttcagcg tgtatttgtt
     3181 ctccgttttt tccgaccagg gcatgtcgag tatccagtcg atgtaattcc ggacaacggt
     3241 ggcttccgcg gacatggggg tcatcatttg cagctttttg agttcctgct tgactttttt
     3301 gaccgcctcc tcggacattt tcttctgctt caggcgtttt tccaactcgt tgatctcatt
     3361 tcggaaatcg tctttttcgc ccatttcctt ctggatggcg cgcatctgct cgttgaggta
     3421 gtaatccttc tgcgtcttct ccatttgttt tttgacgcgg cgcttgatct tttcctcgac
     3481 ctgcagaatt tcaatttccg aaagcatcag ggaataaatc gcttccagtc tctcgctgat
     3541 atcaaatatc tcaatgattt tttgtttgtc ttcgagtttg acattgagat gagtgatgac
     3601 cacatcggca agctttgaag catcctcaat cgccacaacc gtgcccagca tttcgacgtg
     3661 aatttttttg ctgagtttga ggtacagttc gaaagattct ttgatgctgc gcatcagggc
     3721 ctgtttcttg acgtcgttgc cgtcgtcaat atcgtccact tcgttgactt tcaccagaaa
     3781 aaaatcgctg ttcggaaggt attctttgat cacgcctctg gtcttgcctt caaccagcac
     3841 cttcaccgta ccgtccggca gacgcaaaag ctgaataatg atgccgatgg ttccgacatg
     3901 ataaatatct tctttcttcg gatcgtcatc cttggcgttc ttctgcgcga ccatgaaaat
     3961 actcttttcg tatttcatcg ccgactccag tgcggcaatg gatttctcac ggcccacaaa
     4021 cagaggaaca atcatatgag gaaacatgac aacatctctc aacggcaaaa gagggatgat
     4081 ggccgttttt tcctgttcgt tttctatctt ttcatcaaac atactttccc tttactgcat
     4141 ttaagcggat tccgatttgg attcgtaaat taaaattggt ttttcttttt tcagaacgac
     4201 gtcttcattg attacacatt ctttgatatt attttgcgat ggaatgtcat acatcacttc
     4261 caacatggta ttttccagaa tcgaacgcaa cccgcgggcg ccggattttc tctccgtgga
     4321 taagctggcc accgccttca aggcgccgtc ggtaaatttc aaaaccacgt tttccagcgc
     4381 gaataatttc ttgtactgct tgatgatggc atctttcggc tcgaccagaa tcttgatcag
     4441 cgcggcttca tccagttcat ccagagtggc aatgacgggc aaccggccga tgaattccgg
     4501 aatcagaccg aatttcagca aatcttccga tcggacttca ctcaacagtt caccgatgcg
     4561 tttttctttc ttgcttttaa tttccgcgcc gaaccccatg gcgttgacgc ccagcctctt
     4621 ggagatgatg ctttccagat cattgaaagc gccgccgcag ataaacaaaa tattggtcgt
     4681 gtccacctgg ataaattcct gctgaggatg tttgcgaccg cctttgggcg gaatattggc
     4741 catggtccct tccatgatct tcaaaagggc ctgctgcact ccttcgccgg atacgtcgcg
     4801 tgtgatggac ggatttcccg acttcttcgc gattttatcg atctcgtcaa tgtatacgat
     4861 tcccttcgac gcacgggcga catcgtaatc cgcattttgg agcagactta aaataatatt
     4921 ttccacatcc tcgccgacat aacccgcttc ggtcagggta gttgcgtcgg caatggtgaa
     4981 cgggacattc agaaacttgg ccagagtctt ggccagcaag gttttaccgg agccggtggg
     5041 accgatcagc agcacattgc ttttctgaat ttctataccg tcggattcga cgcgcgaaaa
     5101 aagtcgtttg taatggttat agacggcaac cgacaaaatc ttttttgttt tttcctgacc
     5161 gatgacgtac tgatccagaa attttttaat ctctttcgga tacggcaagg ctttttgagc
     5221 gtctttttca atcagcttat cgtcttcttc ttcaacaata taacggcaga gttcaatgca
     5281 ctcgtcgcaa atataggctg tcggccccgc tacgagcttg cgtacctcgc tttgcatttt
     5341 cccgcaaaag gaacaaaaaa gcattccgtc atccattctg ttattcttgc ctttttttat
     5401 cacttatttc tccttggccc tgacggctga agcatccggc tttctgataa tgatttcatc
     5461 aattaaaccg tattctttgg cctgttcact gctcataaaa taatcccgct ccgtatcatt
     5521 gactatttta gccagcggct gttgtgtcaa atcggccagg attttgttca actcgtcctt
     5581 taatcttaat atttcttttg cctgaatgtc aatatccgtt gcctgtccct gaaaaccgcc
     5641 cagaggctga tggattaaaa tccgcgaatg cggcagtgcg aatctctttt ttctttctcc
     5701 cgccgccaga aggagggctg ccatactggc cgcctgtccc atgcataccg tagaaatcgg
     5761 tgatttgatg tagcgcatcg tatcataaat ggccatcccg gcactgacaa tgcctcccgg
     5821 tgaattcaga tagaagaaaa tttccttgtc cggattttcg gcttccagat aaagcatttg
     5881 agcgacaatc agattggcca cattgtcgtc gatcgccgta ccgagaaata cgatacggtc
     5941 tttcaacaaa cgggaataga tatcgtatgc ccgttcgccc cggccatcct gttcaaccac
     6001 cataggaatc aacgtcacga tttcgcctcc atattcagtc cgactttttc cacgacctta
     6061 atatttgcat tctgttcaat aaagtcaaat accttttttt gaaccaattc catcatcagg
     6121 gaatcttttc tttcctcaat attatacatt ttctggaacg actcgtaatc tctcccgctg
     6181 tgttgcgcca gttcccgaag atagctttcc agatcgccgt ccgtcgcggt aatggattct
     6241 ttttccgcaa tcttctttaa caccaggaaa gatttaacga ttttttccgc gtcgggtttg
     6301 tatttgtcct tcattttcag gttgaagtcc atggcggtat tctcatcgat accggcagag
     6361 agcatttttt gctgcgtttc catcatcatg taatagatct gccgttcaac cagcgcggag
     6421 ggaacttcca attcattttc gctgaccaga atgtctgtta ttttgttttg caacttaacc
     6481 ttgtccatct cgcgggcttt gtcttccagg gttttccgga catcattctt gaaatcttca
     6541 aaagattcat atttttcaaa atttttaatc aaactgtcgt cgttttcggg caattttttt
     6601 tctctgatgc tcttaacggt aacgtgaaat accacttcct ggccggcaaa tctgctttca
     6661 tgatagtccg tcggaaacga tacgtgaatg tctcttgctt cacctttttt cattccgatc
     6721 agctgatctt cgaaaccggg gacaaatttc tgggatccga tttccagaaa ataatcgtct
     6781 gctttcagtt ccttgtattt ttcgccgttc aaagttcctt cgaaatccat cacaacgaaa
     6841 tcacccttat ccgccgggcg gtcatcggtc acttcctcca tggtggcaaa catctgcctt
     6901 atttcgtcga ttcttttttg cacgtcatca tcggtcactt taatctgttc tttctccacg
     6961 tctatgcctt tgtaatgacg gggctcaaaa tcctgttccg tttcgaaaga cgctgaaaaa
     7021 gaaaagttag tattatcctt gataccgtcc tgattgattt ccggtctgga aacgggcaca
     7081 atcccctttc cttccaaagc ctgccagtag tatttattga caatattggt gatggtctct
     7141 tcttccgcct gatctttaaa atatgtttcc agaattttac ggggaatttt gccggggcga
     7201 aagcctttaa ttttagcctt tttacccact tcctgataaa cggcatccag ttcattttta
     7261 acgtcatccc agggtatttc cagtgacacc ttctttttga ccgggcttaa atcctcaaca
     7321 tttataacaa cctgactcac tatcccttct cctttatcgt tgaattatgc tttttcgttt
     7381 tttaaaatct ggtgcgagag aggggagttg aacccctatt ccgcatcgcg ggctggatcc
     7441 taagtccagt gcgtctgcca gttccgccac tctcgcatgg acattcttat atattcactt
     7501 agtttaagat tgtcaacgca gattattttg caaaatcaca agaaatttca tctatttaag
     7561 attttacggc cccattattt atttgattgt ctgcttttat gcaagaaaaa ataaattttc
     7621 ttttccggac gctcatcgta cagaaaccag atgaatcgcg aaaggatgcc ttgtcgaatg
     7681 cccatttcat tcaaccggta tttaaacaca aagggcaaac cggataggtg gtttgcccct
     7741 taaattaatt ggtcggggcg agtggatttg aaccaccggc cctctgaacc ccattcagat
     7801 acgctaccag actgcgccac gccccgatga aatacaaatt gacgtgcgtc attaccatta
     7861 cggccaaccc ctgtcaaggg taaataaatt gacgcttatc gcaacgatat ttttatgtcg
     7921 tgagatggat tattcccctt cattcataaa aaaatcttaa taattaattg acactttttg
     7981 tgtttttatg cctataataa aaaagttaac atgacacaaa ggagtttttt ttgaaactaa
     8041 gagacgttaa agaaatattg gacgcccatg tcgttgtcgg tgaagaaaaa ctggacatgg
     8101 atgtgcatac ggctttcggc gccgatttaa tgagtgatgt tatggccttt gccaaagcag
     8161 gcagcctttt gctgaccgga ttgaccaatc cccaggtcat caaaaccgct gccagtcgaa
     8221 atatcgccgc catcatcgtt gtccggggaa aagagcccac aaccgaagcc atcgcaatgg
     8281 ccaaagatct gcaaattccg attttaacca caaactatat tttatttgag acggccggca
     8341 ttctgtacag taaaggaatt gtgggctgtc tgaaaaaagt tgaaagcggt ttttccaaat
     8401 aaaacggttt cccggttttt ctcagaacat tcaaaatatc gaagatccat tttttaagtt
     8461 atttttaaaa gaatcacttg aaaacacgtc ggtgcgtgtc gaaatctctc ttttgctgtt
     8521 tgtttttttt gattttacca aaatgatttc attgacagta aagtctcttc tatgataaaa
     8581 gatttttaat ttaattttag atatttaaaa caaaaataac ctttttcagc aaggagaaaa
     8641 tattgaagct tcgtgaagtc aaggacatac tcaatgccga tgtgattgtt ggagaggaca
     8701 agctggatat cgaggtgcaa acagcttttg gagcggatct gatgagtgac gttctcgctt
     8761 tcgccaaagc gggaagtatg ctgcttacgg gactgaccaa tacacaggtt gtccgaatcg
     8821 ccaatgtcct ggatattgcg gcaatcattt tagtacgcgg caagaaactt tccaccgaag
     8881 cgatttctct ggctacggat cttcatattc cgattttaac gacaaaatat attttatttg
     8941 aaacggccgg tcgtctttac gcgaaaggta tagtcggctg cctggagaaa gttgaatccg
     9001 gcagtgatcg aatctgacaa aaaccagcac gtattcacgt ttgacgttga aggaggtaat
     9061 ttcaatgccg cgggaactgt ttccacccgc attaaatccc ttctgaagca attacaaatg
     9121 cctgatgatg tggtcaggcg gtcagccatt gtttcctatg aagcggaaat caacattgtc
     9181 tcctacgcaa aaaaaggggt aatccggata acggtcagtc ccgatcaggt aatgatcgaa
     9241 gccgacgata cgggtcaggg gatacccgat atcaacctcg ccatgcaaca gggctattcc
     9301 acggccaatc atctgatcag ggaaatgggt ttcggcgccg gcatgggatt gttcaacatc
     9361 aagtcgtact cggacaagtt tgacatcaca tcggaagtgg acaagggaac atttttaaga
     9421 attatcatta aagaacacgt tacataaagg atggattatg gacatcggaa tcatcgcaga
     9481 aaaattaaac ctcaaattca tgtcggcgtc cgacagaaaa gtgaataacg ttacaggagg
     9541 gtacacgggg gatctgttaa gcgatgttat ggccaattca cacggaggag acgtctggat
     9601 caccagacag attcatcaga atatcgttgc cgtggccacg ctgaaagagc atgccggcat
     9661 cattctagtc aacgcctgcg agccggccaa ggacacgctg gaaaaagccg ttcaggagaa
     9721 cgtgccgata ctggtatcag acctttcagc atttgaatta accggcagaa tctataactt
     9781 gctgcattcg gataagtaag agcgaatttt ttatgtctcc ccggcacaaa gctgcctgat
     9841 gttgtactgt cccgtccgga aaccatgatg aaagttttta attgcgacct tcacattcac
     9901 acctgcctgt cgccctgcgc agaactggac atgcatcccg ccgctttagt ggaaaaaacc
     9961 attgacgccg gtctggatgt gattgccgtc tgcgatcaca acgcttcaga aaatgttccc
    10021 tatgtgatca aggcggcaca aggcacgcac ttgaaaattc ttccgggaat ggaaataacg
    10081 accagcgagg aagtgcactt tcttgccctg ttcgattccc tttccgattt gatcgccctg
    10141 cagcatttcg tcgatctgca tctcccggga aaaaatgatg aggaccggtt tggggttcag
    10201 gccatcgtca acgaacacgg cgaggttgaa ggactcagcg ataaattatt gatcggagct
    10261 acggatgttg cgttgaatga tttgctcacc gtcgttcatc atcatcacgg actggcgatt
    10321 gcctcgcaca tcgacaggga aagtttcagc gtcctgggac agctcggttt tatcgacgac
    10381 agcattcatt ttgacgctct ggaaatatcg tatgcaaccg ggattcggca ggcgcgggaa
    10441 agatacgcgg atttgaaaaa atacgctttc atcacctcat ccgacgccca ttatttgaag
    10501 gacatcggca gagccacaac gaaaatcatg ataaacgaac cgacgctgtc cgaattgaaa
    10561 atggcttttt caagggaaaa cggccgttat gttgtggaat agtccatgcg acagcatcct
    10621 ctgtatgcat cgtcgcatcg ttgaccggac ccgacctttt tccgctgatg cggccgagct
    10681 gttcatgccg cgcttttaga cttcatgcct tacgcaatcc tgaaggcatt tttacagatc
    10741 tcgtcgggtt ttgcgatttg cttcggaaac ccggagatcc atgaaacggg agatttataa
    10801 ctcatgctgg aacttgccgc acacattctg gatattgccg aaaactctgt tcgggccggc
    10861 gccaaactgg tgaacatcac catcatcgaa gacgacaaga aaaatctgct ttccattgaa
    10921 atcaatgatg acggacacgg catgaccgaa gaggaaattg tcaaggcgct tgaccctttt
    10981 tacaccacaa aaaaagtcag acgcgtagga ctgggccttc cgcttcttgc ggaagccgca
    11041 aaaagagcgg gcggcgacct tcgtctgaaa tcggtcaaag gcaaaggaac aaaactcaaa
    11101 gtcacctttc agctcgatca cctggacagg caaccgatgg ggaatatcac tacgacaata
    11161 cgcaatctta ttgccggaaa ttcttttgtt gactttcttt ataaacatag gcataatgac
    11221 cgccggttct cactggatac gagaacaatc cgcaaggaaa tcgatgattt accaataaat
    11281 catccggaaa ttttaaagta tattagggat gttatcgatg acggtttaga ggatattcaa
    11341 tcccgcgcgt aacagaccaa taagattgag gaggaaattg atgaagtcgg aagataatga
    11401 actcttgaaa gaattcactc cagaacaggt ggatcagcta aacaaaatta ttaagaagca
    11461 caaaggggaa ccggggagtt taattccggt tttggaagaa gcgcaggtat gtctggaata
    11521 tctgcccgtt tccgtgcaga agaaaattgc caaagaactc aacctgcctt tgagccgcgt
    11581 ttacggtgtc gttacatttt attcattctt caccatgacg ccaaggggta aacataccgt
    11641 ccgagtctgt ctgggaacgg cgtgctatgt gcgcggagga aaggcactga ccgaaaccct
    11701 gcagaaggaa ttcggaattc acgaaggcga aacgacggca gacagaatgt ttacgctgga
    11761 gtccgttcgc tgcctcggcg cctgcggtct cggaccggtt gtcgttgtgg atgaagatgt
    11821 ccatggacgc gtcaagccat ccaagttaaa agaaatacta gcccaataca aataaaataa
    11881 aaaaagccgg gaggaaacgt caatggctaa attgaccata aatgacttaa aaaaaataaa
    11941 ggaaaaagtt cataaggaaa tgtctctccg agacggcgaa cgtcgtgtaa aagttaccgt
    12001 gcatatggga acctgcggca tcgcctcggg agccaaggaa gtgatggaca cgctgcttca
    12061 ggaaattgaa acagccggag ccaatgatgt cgtcgtcacc acatccgggt gcatgggatt
    12121 atgcagccgc gaaccgctgg taacggtaga aatcctgaat caggaaccga tcaaatacga
    12181 atatatgaat tcgaacaaaa tgagacaggt tttcaagaga catattcagg gcggagaaat
    12241 tcagacacct tttgtgctgg cccggggagc tgaagtaatc aaataatatt ttccatcgat
    12301 aatgcttgtt gccacaaagc acgatgattt cttaaggagg atgtaggcta aatgaaagtt
    12361 ttacgcgcac atttactgat ttgcggaggc accggctgtc aagcttccgg gagccctgcg
    12421 gtaaaaaaag ctttattgga agagcttgat aaaaagaaat taacggaaga gattaaagtt
    12481 gttgaaacgg gatgcaacgg cttttgcgcc ctcggcccca ttatggtcgt ttatcctgaa
    12541 ggcgtcattt atatatccct gaaacccgag gatatggcgg aattggtgga ggaacatctg
    12601 gtcaaaggac gaatcgtcca gagacttctt tatcgtgaac cgacaacgga tcagattatt
    12661 cccacgatgc aggacatccc cttcttcgcc catcaggaac tcagagtcct caagaaccgc
    12721 ggtctgattg atccggaaaa aatcgaggaa tacatcgccc gcgatggata cgccggcatg
    12781 gccaaggcgt taaccgaaat gacccccgaa cagatcgtgc aggaaattct ggattcggga
    12841 ttgagaggac gcggaggcgc cgggttcccg acaggtctga aatggaaatt tgcagcacaa
    12901 tccaagggcg acgttaaata cgttctttgc aatgccgacg agggagaccc cggagcattc
    12961 atggacagaa gcgtcctgga agccgatccg catgcggttc tggaaggcat ggtgatcgcc
    13021 gccaaagcga tcggatcaaa acacggatac atctactgcc gcgccgagta tccgctggcc
    13081 attcaccgtt tgaatgtggc catcgatcag gccaagaaag ccggcctttt gggcaaagat
    13141 attctgggca cgggatttga tttcgatctg gaaatttatc agggcgccgg cgcatttgtc
    13201 tgcggcgaag aaacggctct gatgacgtct atcgaaggca aaagaggtat gccgcgcccc
    13261 agacctccct tccctgccgt tgccggtctg tggcagaaac caagtatttt gaacaacgtg
    13321 gaaacgctgg ccaatatcgg acagatcgtc ctgcgcggtg ccaaatggta cgcctctatc
    13381 ggaacggaaa agagcaaggg aacaaaagtc tttgcgctga ccggagatgt ggcgaatgtc
    13441 ggtctggtgg aagttcccat gggaacaaaa ttgggaacta tcgttcacga tatcggcggc
    13501 ggcattccca aaggcaagaa gtttaaagcc gctcagttgg gcggaccgtc cggcggctgt
    13561 attcccattc agcatttgaa cgcaccggtc gattacgaga aagttgctga actaggcgcc
    13621 atcatgggct ccggcggtct gattgtcatg aatgaagaca agtgcgctgt ggacatggcc
    13681 agatttttca tggatttctg tcaggatgaa tcctgcggca aatgtacacc ctgccgcgag
    13741 ggaaccaaac gcatgctgga aatcctaacc aatattaccc agggcaaggg aaaggaaggc
    13801 gacatcgagc ttctggaaga gatggcccag gttatcaaaa acgccgcttt gtgcggtctg
    13861 gggcagacgg cacccaatcc cgtattgagc actattcgtt atttccgcaa tgaatacgag
    13921 gagcatatcc gcgatcatcg ctgccgcgca gccgtatgtt cggcaatgtt caaatcaccc
    13981 tgtcagcata cctgtccgat cgaaatggat atccctgctt acatcgctct gatccgggct
    14041 gaaagatttg acgatgccta caaaattctt cttcagacca accccttccc ctccgtttgt
    14101 ggaagggtct gcgatcataa atgccagaca aaatgccgtc gcggcaatct ggatgaaccg
    14161 gtcgccatta aattcctgaa gcgattcatt accgacaacg cccccagacc caaggtcgac
    14221 cccgttttgg tcagcaggaa agaaaaaatt gccgttatcg gtgccggtcc agccggttta
    14281 acggccgcgc gcgatctggc tttacgcggc tacaaggtga ccgtttttga agaactgccc
    14341 aaaccgggtg gaatgcttta ctggggcatt ccgtcctatc gtctgccgcg caacattctg
    14401 gacggtgaaa tcgacgatat ccgtgcgctc ggcgtggaca tcaaactcaa cacccgggtc
    14461 ggcaaggata ttaccttcgc caaactcgaa aaagaattcg attacgtcta tctggccacc
    14521 ggcgctcaca agagccagaa gatgggtgtg aaaggcgaag atctgaaaaa tgtctacggc
    14581 ggcgttgagt tcctgagaga ttttaatgcc aatgaagaaa aatggctcaa cggcaaaaat
    14641 acgctgggca agaaagtcgc cgtcatcggc ggcggtaatt ccgccatcga cgcagcgcgt
    14701 gtcgcacttc gccttggatc tgaagtaacc attctttatc gtcgcttgag acaggatatg
    14761 cccgctgccg aggaagaaat taaggcggcc gaagaagaag gtatcaagat tgattttctg
    14821 gtcgctcctt tgaacgtcga aggtgcaaaa ggtaaagtga caggcatcac ctgtcaacgc
    14881 atgaaactgg gcgattttga ttccagcggt cgcaagaaac ccgttccggt tgaaggatcc
    14941 gaattcaccc tgaaggtgga tgcggtgatc tcggccatcg gccaggtccc ggatatgagt
    15001 ttcatcgatg ccaagcgagg cgtggaaatc aataaatggg attgctttgc tgtcggcaag
    15061 gtttacaaat ccggcacaac caacccccgt tatttcgccg gcggtgacgc tgtaaccggt
    15121 ccggataccg cgatcggagc cattgctgcg ggacatcagg ctgccgacga tatcgatgcc
    15181 gccattcgca aagcgaacgg tgaaccggct tatgaaaaac cggcacttga aaaaatcgat
    15241 atcccgctca ttatcgatga agaaaccgtt gaagctccgc agatggcaat gcctgaactg
    15301 catcacgaaa cccgtaagac gaatttcgtg gaagtggagc taggcttcaa acgcgaagac
    15361 gctattaaag aggcgtgccg ctgcctgcgc tgcgacgcgg ctgtttaatt tactctaaag
    15421 gtaatgcaaa aaaagaggct tgcccttgtc gggtaggcct cttttctttg gtcgatttct
    15481 aaaaacaaag actgtggaag aagcatccga attgttgatt aagacaattt gaattttaag
    15541 aatttgtttg tgcttttctg cgttaagaat ctgttcaatt tatagtttgt tcctttcaag
    15601 cctgaaaaat accccagtgc atacaacgtt tcattcttcc tgatatcgac tctgccggca
    15661 ccctgcaatt tcttaatggc ccccacctga cacaacgcct taaaatattt ctggagggtg
    15721 atttttttta tatctaacgc ctgaagaatg gcgttgtaga aatcctggtt tgtgataaaa
    15781 caggcatagg gatatctgcc ttgatatttc ggttcctcca gttgaaacaa atagcggaaa
    15841 ggttttttgg ttttggcgct tcggctatct ttcttttcaa gctcattaag aatttcacaa
    15901 accttgtatt ttataatcaa ccgctccacc ctcggatcgt cattcatcca tttccgggca
    15961 tatccgagct tctttccccg taattgccga tgatagacgt tctcgatatg ttctttgtaa
    16021 ttgattaata cactgtaaaa cgtcgcaatc gcctgggact ccacgtaatc aagccattcg
    16081 cccttccctt cgtcctcgtc atccgtattt ctttcctgcg tcgcctggtc gatccatgcc
    16141 cggaatatgc cgtcaaattc agcctccaga gaatctattt ttttatgatc gtccgcaacg
    16201 cttgatgacg tgtcttgcac cgtttgttca tcatccttaa tcaggtaatc gataaatgcg
    16261 gaaaaatcgg caaactcctt ttgatagtgc attttatatt tattgtgtgt cgaggtgaca
    16321 aatttttcat aatttccatt ccagctctcg aataattctt tttgagtttc aaaataatgc
    16381 cggagatttt ggtcctgccg gtttcccaga ttttcttttt ttttgcggtt attttccatg
    16441 cctgcttata aacctccaaa ccagtatccg tataaaatat cgacaacgtt ttttttcaat
    16501 tgtgttgacc ggtttaaaaa aaatattcct cttgcacaat catctataag gaccttccat
    16561 cgggatgtca attcttttga ggtaactata agttcttgaa cttattcatg acttacggtt
    16621 cttcatatat atcacaaatt attacctttt ttttgacaaa tttaacaatt gagcgtataa
    16681 ggaatcgtta aatttctggc aacaactgcc ctgagctctg cgtgtataag caatgattca
    16741 ttcatgcata catgacaaag accggcagta aaataacatt gtttcaaaat attcagaaag
    16801 gagatgagat catgccacga tggacgtaca aaacaaacat tattgatctg gatcaggtga
    16861 tcccgcccga caaagtccaa tgcgacctgt caggagactg cattgtcacg gatcttcagg
    16921 gaggaggcct tccgcatttc aaggaaatgc tcgacaagga aggcgaaggc gaatgggagc
    16981 ttgtgcagtg ccagtatcac ggaggcaaac tgctgtgcat ctggaaaaag gaaattaaag
    17041 aagcctttgc cagttaatca atccggattg gtctgtcatc agcccttccg ttgagcgcgc
    17101 ataacgatag aggctgatca gggatgaagg tgaaaaaaat ccagtcatgc ggctttgatg
    17161 cgtcgtacgc tggttatttt aatgaatgga ggtagtagtg tcatggcttt taatgcacca
    17221 gccaaaatgg taggtcttgt cggcaatgcc ggcgtttaca aatcaaaact ttcagtattt
    17281 caatttctta tccggggctt catggcagga gcctacatcg cgatcggcgg tgcgctgtgc
    17341 accgtgtgtt cgaccggtgt agcgcccatc cttggccccg gcatcggcaa attaatcggg
    17401 ggtgcggtgt tccccgtcgg attgattgcc atcgttctca ccgggatgga actttttacc
    17461 ggagactgta tgctcgtccc catggccgca atgatgaaac ggtgtacatg gggttcggtt
    17521 ttcagaaact ggttctgggt ctatatcgga aacgttgtcg gttctttttt ctatgcctat
    17581 atgatggcgg ttggcccgtt tgtggacggc accgcggacg gcacgctggt cgtcaacgcc
    17641 ttcggaatga cggcgatcaa tatttccgtg gccaagattc tgacgtacaa ggccgtcggg
    17701 accgccggcc tctggtcctg cttcatgaag gccatcggtt gtaatttcct ggtcaacatc
    17761 gcaattttgc tggccattac ggcggatgac gtgatcagca agtttttcgg aatctggttc
    17821 ccgatcatgg ccttcgttgc caccggattt gaacactgtg tcgccaatat gtatttcctt
    17881 cccacaggca agttcctgac ggactggtat ccggccacca tcgaaaaagt cggcggcctt
    17941 ttggcagcca acggcggttt aagctggggg gacatctggt tctggaacat cattccggtt
    18001 accatcggca atattgtcgg cggatttctt ttcatcggtg tggcctattt tctgatgttc
    18061 agaaatgaac ttccgaaaga ctagaggaat cgcaattgtc actaccggca tggataactt
    18121 tttttatctg cgcggcggcc gtattcattc actatatgca ggtgaggaat acccggtttt
    18181 tgctctttgc cattcccgct ttgctcgctc ttctcattat tccgatgctt ttggcatgga
    18241 tgagtcggag aacgttaaat aaagcggctc aagaaaacgt gtccaaggca aagttgtaca
    18301 aaatcggcaa agtgactccg gccatgacgg gacaggttgt cagcatcaaa ggccatgtca
    18361 aaaaaatcag ctttcgatgg ctcaacaggc cgcattttca tgttgaagac tcaacaggcc
    18421 ggatccgggt gattatgttt accgcacccg atgacaatat ccgagaggga gaccccgtag
    18481 acatactggg aattgtaatc aaaaatattt tcaaacgcgg caccccggcc atatcggccg
    18541 tcagcgttaa gaagaacgaa tagataacag acactttcag gaggtcgtca tgaattcaag
    18601 agaaatcatc tttctaatag gatttttaat tctgggcata acgccggttt tcgcgcaaca
    18661 gcttcccggc ggagcaacgg aattgatcat gctcgcaggc atgctgattt ccacatccac
    18721 agcttttgtt aaatcgtaac atccgggcag agcgaagcgg cggccggtcg tcttcgctct
    18781 gcccctccat gatctttcca taaaacagga aattgcttac cggattttgg ctaatttatt
    18841 tttgttagtg ttttcatcaa tcgacacatc cgtacatgcg ccgatctcat tgcatctttc
    18901 catcggccgg gtttgaagcc ggaatcgtgg cttcttatct cttctaaaaa gaacggcttg
    18961 cttttttgct caaaaagaaa gtttcgagcc ttatgaatca atataatttt ctaatattat
    19021 tcataacata aaatcctttc catccctccg agaaaacacg actttgtgtt tgacaaaata
    19081 atgtattgca gctataagcg gcagtcaaac tgctgactga taagttttcg aagtccaaag
    19141 agaaatttgt cccattggaa gcccgttcag tcgtattaca ggcagctcaa tataagaaga
    19201 agttataata aatttactaa agtgaggtaa ggaatttatg gcaaatgaaa atttagaaag
    19261 taagaggtgg ttgattgccg gcgcggctgt tatcattcag ttgtgtctcg gcaccgtgta
    19321 cgcatggtcg gttttcaaga atcctttgat gcagatgcat ggatgggaag gcaaatctgt
    19381 tcagtacacg tttatgctgt tgatgctgat catcggatta gcggctgctt tcggcggtac
    19441 cttggttgat aaaaagggac cgcgttttgt cgccaccatc ggcggcattt tattcggtat
    19501 cggcaccctg gtggccgggt acgcagatca ggttgggaac atttatttgc tttacctggg
    19561 tttcggcgtt atcgccgctc tgggtaacgg tttcggttac gttacgccga ttgccacgct
    19621 cattcgctgg ttcccggaca aacgcggtct ggtaaccggt cttgccgtga tgggtttcgg
    19681 cgccggtgct ttcttcatgg ggaaaattgc tcccgtgatg atcaaatcct tccagcagat
    19741 cgaccccgca ggaaccatga tcgcttccgg cgttgccaac acctggtata tctggggtat
    19801 catattcctg attcttgtta caggttccgc tcagttattt aaaaatcctc cggccggttg
    19861 gctgcccaaa ggattcacac ctgccgccac aaccgtgtcc gcggcggatt cctttacctt
    19921 cggagaagct gtgaaaaccc gtcagtggtg gatgctctgg gccatgctgt gcctcaacgt
    19981 ttccgcaggg cttggcttga tttcccagca ctctcctctg gcacaggata tttacaaaaa
    20041 aacttatggg ttaacgggag acctgacgcc tgaacaagtt gcgatcgttg ccgccgccgg
    20101 tggcgctgtt gtcgcttacg ccgccatctt
//
LOCUS       DAOOLB010000034        19101 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_2928_length_19101_cov_30.095348, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000034 DAOOLB010000000
VERSION     DAOOLB010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 19101)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19101)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19101
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2928_length_19101_cov_30.095348"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="PLF77_05920"
     CDS             complement(<1..391)
                     /locus_tag="PLF77_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08425.1"
                     /translation="MNYLVQPKKYQSLMTDDGSRAIMDKTVAFFEKMGKERLLDDYNK
                     KVWYREFVDFIGREQIFAKLLTPSQYGGGDPDCRWDTARNSEYSELLAFYGLGYWYCF
                     QVSILGLGPIWMSPNEKAKKKAAELLKK"
     gene            complement(643..1113)
                     /locus_tag="PLF77_05925"
     CDS             complement(643..1113)
                     /locus_tag="PLF77_05925"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPC08426.1"
                     /translation="MENIKKILAVSWITQYCQATVRAAIEVASKFDAELSVIHVVDTQ
                     LLQGWNLPFKFHDEENQKELERIKSEINSVVNSEKKEDMKVKTVVKDGNPVEEILKYV
                     EKEKVDLVVLRAHEKSRLEKFMVGSSNDEIILKMPCSILLLKNEPKKKTIRFKA"
     gene            complement(1237..2025)
                     /locus_tag="PLF77_05930"
     CDS             complement(1237..2025)
                     /locus_tag="PLF77_05930"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPC08427.1"
                     /translation="MKDLNAPANPLETLAYIIAQQIHDHSIVYIGTGIPMVAGILAKK
                     THAPNITIVYESGGQDPIEGDMPWSVGCPFTWRKSPTIMEMANSFAQCYNGYVDIGFL
                     GFAQVDMYGNVNTHQIGKDFHKPKVRMTGSGGNNDLSSLTENMVLVGLQTPDKFPDAV
                     DFITSVGHLKGGNSRQEAGLLGSGPVAVISPWGVYDFHPVSKRMRVKSLTPGVTFEIA
                     QSLTGFELLKPDGDIPQTKLITDDALKIIRTEVDPRGVFTTMPT"
     gene            complement(2040..3131)
                     /locus_tag="PLF77_05935"
     CDS             complement(2040..3131)
                     /locus_tag="PLF77_05935"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPC08428.1"
                     /translation="MSKYVYNQEAVENLRLSDTSILDRLIDFESARAAHLKKDHAKKD
                     KRMSLTEAISTFIQDGDIITDAGFSYVRTPHQAFHEIIRQGKKNLQMIGAPNTNQSFQ
                     IIYGCVYYSHNSYIGVEMRGIDKSYDRMLKAERVKILSEWSHGGVAQGFKAAQLGMPG
                     VFSKQMLGSDIVRYNPYLKVMQNPMKDKNDPVVFIPALYPDVAIIHCHAADKYGNAYI
                     YGPAVNDLAVAAATRKLIITAEEIVPENTIRYSKEGQIIPFFYADAVVELPFGAVPGN
                     MPGCYYWSRQWWEKLIRLGGVSDENMKAILDEWILGTKDQFDFIDKLGGSRWIVEARR
                     QTKAAEGDNEDIDFSYDEYTLNHDSGLYY"
     gene            3413..4045
                     /locus_tag="PLF77_05940"
     CDS             3413..4045
                     /locus_tag="PLF77_05940"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPC08429.1"
                     /translation="MEFSLLQNILLGLSLAAPFGPVNVEIVKRGLNSGFKQAFVTGLG
                     AMTADTTYLTIIFFGLVSFLKMPLLKIFFALAGSLILIYLGVTSIKDFLGKTDYDEKQ
                     NRRFFKNPYVAGYVIALSCPITIVWWTGVFGALLASQDSIQTNFSAYLSCLTILLGCF
                     IWIVFLSAALHWGRKIVNEKFIGFVSLIAGMCLAGFGLAFLCRSVHLIIH"
     gene            complement(4080..5519)
                     /gene="gltA"
                     /locus_tag="PLF77_05945"
     CDS             complement(4080..5519)
                     /gene="gltA"
                     /locus_tag="PLF77_05945"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPC08430.1"
                     /translation="MKNISVGAMETKETKPKKEKVPRQTMPEQDPKERIHNFEEVPFG
                     YSEETAIIEAQRCIQCKKPGCVSGCPVDVKIPEFIKAIAEGRFIDAALKLKETNGLPA
                     VCGRVCPQEDQCEKECILGKKGEPVAIGRLERFAADYERNSGKISIPPAVQSNGKKIA
                     VAGAGPAGLTIAGDLVKLGYDVTIFEALHKAGGVLVYGIPEFRLPKAIVEAEVDYLKK
                     MGVKIETNFVIGRTKTIDELFADGFDAVFIGVGAGAPVFMNIPGENLSGIYSANEYLT
                     RSNLMKAYLFPEYDTPIVRGKKVAVIGGGNVAMDSVRTALRLGAERAMIIYRRTEVEM
                     PARKEEVHHAREEGVEFLLLHNPVEYIGNEAGWVTGMKCIKMELGEPDASGRRSPVPI
                     QGSEFVIDVDTVVVAVGTMANPIVPATTTGLETNKKGYILTKDESGLTSREGIYAGGD
                     IVTGSATVILAMGAGRKAANAIHEYLSNK"
     gene            complement(5485..6321)
                     /locus_tag="PLF77_05950"
     CDS             complement(5485..6321)
                     /locus_tag="PLF77_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPC08431.1"
                     /translation="MNKIIEKENLSESVVRMVLEAPVVAQKRKAGQFIVLKIHEKGER
                     IPLTIVDSDAQKGTITIIFQVVGKTTALLAAMAPGDGIQDVQGPLGNPTEIENFGRVV
                     CIGGGVGVGVVYPLIVALKKAGNEVVSIIGSRTKNLLILEEEIKANSDRLVVTTDDGS
                     YGVHGFVSNVLQEMIDKGEKIDRVFAIGPVPMMRVICDVTKPHGIKTIVSLNSIMVDA
                     TGMCGACRVDVGGKTRFTCVDGPEFDGHQVDFKLLADRLRMYCDQEREAYEKHKCGCD
                     GN"
     gene            complement(6473..7312)
                     /locus_tag="PLF77_05955"
     CDS             complement(6473..7312)
                     /locus_tag="PLF77_05955"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPC08432.1"
                     /translation="MQNPYIPYPVVVDKIITEVDTKDIKTFRFKFVNPEDEQKYAYIP
                     GQFGELSIFGKGESPIGIASSPTQKGYVEFTVQKAGVVTSALHEMEEGTVMGIRGPLG
                     NSWPLDFLAGKNIVIVGGGFAFTTLRSLINYMLFEDNRKRFGNITVIYGARNPGLLLY
                     KDELVEWGKRADINLNVTVDKGDATWKGKEGFVPAVCKEVAPSKENAVAVICGPPVMI
                     RFTLPVFFDLGFSKQNILTSLEMRMKCGIGKCGRCNVGSKYVCKDGPVFSLAELDKLT
                     PEY"
     gene            complement(7327..8349)
                     /locus_tag="PLF77_05960"
     CDS             complement(7327..8349)
                     /locus_tag="PLF77_05960"
                     /inference="COORDINATES: protein motif:HMM:NF028488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPC08433.1"
                     /translation="MEKRLIKKDALAGIAKKMTEKFLVWAPVKKEDNVLFEPLQKNAE
                     PLFAYLNSKNAPKNVFFPHTETMMKYTRTPKGMVFSTEENKAVESVLFGVRPCDAHSF
                     VLLDKLFDQEKYKDGYYIAKRQSTTVISLACVKPPYASCFCTSVDGEPVSSDGADILI
                     TDIGSDYLVEFITPKGEKLASYFGDAKADAATETKKNEIAAKAREAITSKIPAHEIKP
                     ILDKNFDNPFWDTIHRKCLACGTCTYMCPTCHCFDISDEVKGSDGVRIRSWDSCMFPL
                     FTKETSGHNPRSSQKERWRQRAMHKFKYYVDMFDCIACVGCGRCVMSCPVNIDIRKIV
                     EDISKL"
     gene            complement(8509..9357)
                     /locus_tag="PLF77_05965"
     CDS             complement(8509..9357)
                     /locus_tag="PLF77_05965"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HPC08434.1"
                     /translation="MKVSYYPGCSLHATGKEYDQSMKAVSRALDIELKEVDDWCCCGA
                     SSAHNTNFDLSIALPARNLIAAEKDAMDVMVPCAACFNRFKTAEHHLKADKDLKAKIE
                     GIVGAKYQGGIAIRNPIDIIFNDIGIDVLAEKVVKPLTGLKPVSYYGCLLLRPPEVCE
                     FENYENPFMLDKMMKAIGADVKNWSYKTDCCGGSLAISKTAIVVEMCDRLMNGAREAD
                     ANCVVTACPLCMANLDMRPSEGKKLPVFYFTELISLAMGLPGSKDTFKSHIFDPRPLL
                     SQLGLI"
     gene            complement(9385..9909)
                     /locus_tag="PLF77_05970"
     CDS             complement(9385..9909)
                     /locus_tag="PLF77_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPC08435.1"
                     /translation="METFLEEVNKKINGVPLQRCYHCRKCTAGCPMTKHMEYNPNKII
                     KMIQNGQRDKVLNSSTIWLCLSCETCITRCPNQVDIASMMDVLRQMAIQQGTVREANV
                     LKFHEAFLAGMKFGGRINEPMMMVQYKLKSGDLFSDALLGLDMFKKGKLALLSPRTKD
                     MKSVRNIFEKTKNR"
     gene            complement(9934..10944)
                     /locus_tag="PLF77_05975"
     CDS             complement(9934..10944)
                     /locus_tag="PLF77_05975"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPC08436.1"
                     /translation="MENIETKLREEANRLLLEKKVDVIVGYEQGTLPLTATPCFITKP
                     EETNKLVWNNLCTQNLAKYVHDLITQHKNSQKRVKPEDRKKKIVGVVARGCTTRSLII
                     HLQERQYGRDEVYIIGVPCDGYVDGKGLKVAIGGADIVDGSIDGGQIKMKTSGGDKTV
                     ALKDVLADNCKTCRFNNPIISDVMIGSPAPAMNPDAEYDDVKEFEGKSIEERWAYFNK
                     EMEKCIRCYACRQACPSCYCPTCFVEQSQPQWVGIGEDASDTQVFQFMRLYHMVGRCV
                     DCGSCISVCPMGVDLRKFLKKLDKDCFEFFGNRAGSSMDDMPPLGKFDEHHDKGDFVY
                     NP"
     gene            complement(10982..11422)
                     /locus_tag="PLF77_05980"
     CDS             complement(10982..11422)
                     /locus_tag="PLF77_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(11204..11206),aa:Sec)
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HPC08437.1"
                     /translation="MAENLATKEWQPKFVAIVCNWCTYAGADLAGISRIQYPTNVRII
                     RVPCTGRINPFYIVKALQAGADGVLVSGUHPGECHYISGNLVARRKFATLKSFLNYIG
                     VEGDRTIFTWVSASEGDRWAQVIKGATEKIKALGPANKLIKKID"
     gene            complement(11460..15428)
                     /locus_tag="PLF77_05985"
     CDS             complement(11460..15428)
                     /locus_tag="PLF77_05985"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPC08438.1"
                     /translation="MGTSPCKAYCPTHISVQGYVALIAEGKFKEALKLIKQDNPFPIV
                     CGRVCNHPCETACMRGKVDDPIDIMHLKQFVADLDLNSDTRYIPEKKESKGKKVAVVG
                     AGPSGLTCAYYLAIEGYDVDVFEALPVAGGWLAVGIPEYRLPKNVLKAEIKIIEDLGV
                     KIHLNTRIGKDIAFDKLKSDYSAIFIGCGTMKSSKLNIPGEDMKGVVHGVDYLLKVNL
                     GEKVSLGDKVAVIGGGNVAMDAVRTAVRTGSKEVFILYRRTRAEMPASPEEIEEAIEE
                     GIEMKFLVAPKRVVGQDGKVTGIECTRMELGEPDASGRRRPVEIKGSEFIVECDAIVP
                     AIGQEADLSFITKESGVSINKWNNVDFDKITYATNVPGIFTGGDVSTGPQTVVKAVFS
                     GKEAAKSIDRYLRGEDMKAGREKDWTKDLADKADLSNFSKVPREKYPLMKPEDRKTNF
                     KEVGLGFSEAQAIAEASRCLSCGVCSECYQCVEACIAKAIDHDMKVEEETIEVGAIIA
                     SAGFEIFDASKRGEYGYGIYKNVVTALQFERILSASGPFFGHVQRISDGKEPKKIAFI
                     QCVGSRDTSCGNSWCSSVCCMYATKEAIIGKEHAKGLEPTIFYMDIRAHGKDFDRFVN
                     RAKDEYGIRYIRSMPSTIKELQQTNNLLITYVNQDGTLTEEEFDMVVLSVGLMPPKEA
                     KQLAASLGVELEEHGFCKTSLENPVQTSRPGVFVCGAFGGPKDIPETVMEASAAAACA
                     EGLLASRRGTMITPVQNPEEKDMRGQGVRTGVFVCHCGINIGGVVDVPAVRDYAATLP
                     SVVYTADNLFTCSQDTAVKMKEVIEEQNLTRVVVASCSPRTHEGLFQENCEKAGLNRY
                     LFEMANIRDQNSWVHMHEPEAATEKAKDLLRMAVAKAQYLKPLKPGQLSVNHQALIIG
                     GGLAGMTAALSLADQGFASFIVEKEDRLGGNYNHLYKTLEGLDTRAHLKGLLDKVYNN
                     PLITVTTGAKIEKIEGFIGNYKTTVKAKDGEKVFEHGVVIVAIGAYENKPKEYLYGQN
                     AAVKTQRELETMIYEKDAKLASVKNVVMIQCVGSRDKERPYCSRYCCSEAIKNALELK
                     EADPSRDITILYRDIRTFAFKEDYYKKAREANIKFICYEEDRKPEVVAAGDKAEVKVF
                     DPILNEQVSLPADLVALSVGVVPNPENAAIGNMLKVPTNQDGFFMEAHVKLRPVDFAT
                     DGVFMCGMSHSPKFSDETVTQANAAVSRACMILTLDYIEAEGKTAFVNKERCSACGLC
                     EINCPYSAISVNVAEGCAEVNAVLCKGCGVCTASCRMNAVDLNGFNNEEVLAQIYNLN
                     EG"
     gene            complement(15450..15908)
                     /locus_tag="PLF77_05990"
                     /pseudo
     CDS             complement(15450..15908)
                     /locus_tag="PLF77_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(16107..16709)
                     /gene="yihA"
                     /locus_tag="PLF77_05995"
     CDS             complement(16107..16709)
                     /gene="yihA"
                     /locus_tag="PLF77_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPC08439.1"
                     /translation="MKITSAQFIKSAVWPPQYPPAIMPEIAFVGRSNVGKSSLMNTLV
                     GRKNLAKTSNTPGRTQLINFFTVNETISFVDLPGYGFAKVPRSVKKDWGDMMEAYLRE
                     RQSLCLVIFILDIRRDPNADDLSLRDWLDHYRIPYLYILTKSDKLSNNQAIARKRAIE
                     KMLRISSEKKTILFSAKTQKGKVEILEFLENHLRALDKHS"
     gene            complement(16748..17641)
                     /locus_tag="PLF77_06000"
     CDS             complement(16748..17641)
                     /locus_tag="PLF77_06000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC08440.1"
                     /translation="MISVIIPTFNRGYTLAQVLDSYYRQQFVAELIIVDDGGSDKTAV
                     VVDEFVRKYPRIKTMYLKNEKRQGAPYSRRRGLLAAHGEFVLFGDDDDFLEENYTTVC
                     LNKLKSLPADIASGRHFFRNAGENVAEAIARFGQGSIDVPPFDFRLFYINTDAKFKSD
                     VELPFTHAIYLVRRELLLSYEMDTFYSRGNGFREENDIQAKIHINGGKIIITNDTHSV
                     HMHSSEVQTGGQRVGALAKLFWSLYYTAYFFKKYYKPIMERQGENGCYLKGIVIYYAL
                     TLLTFPFLVLKEKRILRSMRK"
     gene            complement(17984..>19101)
                     /locus_tag="PLF77_06005"
     CDS             complement(17984..>19101)
                     /locus_tag="PLF77_06005"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC08441.1"
                     /translation="QAMKSGAFDYIHKPVNVDELDLAIKKALKSLEMEKKIDGLLMEP
                     SRSFRVGDIIGTGNEMREIFKIIGTVSQSRTTVLIQGESGTGKELIAKVVHNNTSPDE
                     PFIAVNCSAIVETLLESELFGHEKGSFTGAVARKLGKFELARFGSVFLDEISEMSVNL
                     QAKLLRVLQEMEFDRVGGKEKVKVNARIMAATNKDLKALVKEGKFRDDLYYRLNIVSI
                     TLPPLRARRQDIPPLIDYLLTKINLDLHKKIIGVSDEVMEIFMQYTWPGNVRELENLL
                     VRACVVAKGQVLGVNDFPELGKEEAAGPAGDRDANLFQASEPGKLLTLDEVEERYIRK
                     VIKETDKNKGEICEILGVSRPTLERKLEKYGITFERE"
BASE COUNT         4559 a         4995 c         4373 g         5174 t
ORIGIN      
        1 ctttcttaag cagttcggca gccttcttct tggctttttc attgggactc atccaaatgg
       61 gaccgagtcc caggatgctg acctgaaaac aataccagta gcccaggcca tagaaagcca
      121 gcagttcgct atattcgctg ttgcgggcgg tatcccagcg gcaatccgga tcgcctccgc
      181 cgtactggct gggtgtgagc aatttggcaa aaatctgctc cctcccgata aaatcgacaa
      241 actcccgata ccagactttc ttgttgtaat catcgagcag acgctctttg cccattttct
      301 cgaagaaagc tacggtctta tccatgatgg ctctggaacc gtcatcggtc ataaggctct
      361 gatatttttt cggctgcact aaataattca tctgatcctc ctttttactg gaaacaatta
      421 taatatttga ttatcttgtc tttaagacag cgtcccgaaa tccctttgcg ggtggttatc
      481 ccccgtgtat ggggaaacca ttctgaaaat agaccggtga tagaaacatg aatattgcat
      541 tgtggacatg gcggtccgca tcctgttttt ctaaccgacg gctatctcct gcctgtttaa
      601 cacaggcaga agagctttcc aaggcaaaat tgctctccat cctcaggctt taaaccggat
      661 ggttttcttt ttcggttcgt tcttcaacaa taaaatagaa cagggcatct tcaggataat
      721 ttcatcatta ctgctgccga ccatgaattt ctccagccgg cttttttcat gcgccctcag
      781 aacaaccagg tcgacctttt ccttttcgac atattttaaa atctcttcaa cggggttgcc
      841 gtccttgaca accgttttga ctttcatatc ctctttcttt tcgctgttca ccacagagtt
      901 tatttccgat ttaatgcgct ccaattcctt ctggttttct tcatcatgaa atttgaaggg
      961 cagattccac ccctgaagca attgcgtatc gacgacatga atcacggaca gttcggcatc
     1021 aaatttactg gccacttcga tagccgcgcg aaccgttgcc tgacagtact gggtaatcca
     1081 acttacagca agaatttttt tgatgttttc cataatcgcc ctcctgaatg aatggttaat
     1141 gatatggatt gtgcaaacat gccgttccac caaaaacaat cttcgttttc cggtaatctg
     1201 tccgtatatt tcaaacggaa taaaaacgtg tttccgctat gtcggcatgg tggtaaaaac
     1261 gccgcgcgga tccacctccg tccgtataat tttcagtgcg tcgtccgtaa tcaacttcgt
     1321 ctgcggaatg tctccatcgg gtttcagcag ttcgaatccc gtgagcgatt gcgcgatttc
     1381 aaacgttacg ccgggcgtga gcgatttgac ccgcatgcgc ttgctgaccg gatgaaaatc
     1441 ataaacaccc cagggactga taaccgcaac cggcccgcta ccgagaagac cggcttcctg
     1501 ccgtgaattt ccgcccttca gatgtcccac ggacgtgatg aaatcaaccg cgtccggaaa
     1561 cttatccggc gtctggagcc ccaccagaac catattttcc gtaagcgatg acaaatcgtt
     1621 gtttcctccg cttcctgtca tgcgaacctt gggcttgtga aaatccttac cgatctgatg
     1681 ggtattcacg ttgccgtaca tgtccacctg ggcaaacccc agaaagccga tatcgacata
     1741 accattataa cactgcgcaa aagagttggc catttccata atcgtcggtg atttccgcca
     1801 cgtgaaggga cagccgaccg accagggcat atccccctca atggggtcct gtcctccgga
     1861 ttcgtacaca atcgtgatgt tgggggcatg tgtttttttg gccagaattc ccgccaccat
     1921 gggaattccg gtgccgatat aaacgatcga atgatcgtga atctgctggg caatgatata
     1981 agccagtgtc tcaagaggat tggccggtgc atttaaatct ttcatgggat tctcctttct
     2041 taatagtaga ggcccgaatc atggttcagg gtatattcgt catatgagaa gtcaatgtct
     2101 tcattgtcgc cttcggcggc tttggtctga cggcgggctt cgacgatcca cctggaaccg
     2161 cccagtttat caataaagtc aaactgatct ttggtgccca gaatccattc atcgagaatg
     2221 gccttcatat tctcatcgga cacgccgccc aaacggatca atttttccca ccactgcctc
     2281 gaccagtagt aacagccggg catgttgccg ggaaccgctc cgaaaggcag ttccacaacc
     2341 gcatcggcat aaaagaacgg aatgatctgc ccctccttgc tgtaccggat ggtgttctca
     2401 ggaacgattt cttcagcggt aatgatgagc ttgcgcgtcg cagcggctac ggccaggtca
     2461 ttgaccgcgg gaccgtagat ataggcgttg ccgtatttat ccgccgcatg gcaatgaata
     2521 atggccacat ccggatacag ggcgggaatg aataccaccg gatcgttttt gtctttcatc
     2581 ggattctgca tgaccttaag ataaggatta taccggacaa tatcactgcc cagcatctgc
     2641 ttgctgaaaa cacccggcat ccccagttgg gcagccttga atccctgtgc cacaccgccg
     2701 tgactccatt cagaaagaat tttgacccgt tccgccttga gcatccggtc atagctttta
     2761 tcaattccgc gcatctccac cccgatgtat gaattgtgcg aataataaac acagccatag
     2821 ataatctgaa aactttgatt ggtgttgggc gctccgatca tctgaagatt ttttttgcct
     2881 tgacgaataa tttcatggaa cgcctgatga ggcgtccgga cataggaaaa tcctgcatcc
     2941 gttatgatat ctccatcttg tataaacgtt gaaatcgctt cggttagcga catacgtttg
     3001 tcctttttcg cgtgatcctt tttcaagtgt gcggcccggg ccgattcaaa atcgatcaac
     3061 cggtccagga tggatgtgtc acttaatctg agattttcga ctgcttcctg gttgtacaca
     3121 tatttagaca tctgtttgcc ctcctattca tgcaataaca gttgaatcat cttaccctga
     3181 ccttcagaga gggtcaagta ttatcgtaac aatttgatat aactaaaata tttgttattt
     3241 tttaacggag accaaatgaa gaaaaattcc ccttaaaact gatgtttttg cagcaaaata
     3301 tgaaataata ttttatcaac ctaaaaatgt gaaatatgcc aataaatgga gaatggaaat
     3361 ttacataacg agaaagtgac aagaccgctg cttctgtggt atcaaaaaca gtatggaatt
     3421 ttctttactg caaaatattc ttcttggtct ttcgctggcc gctccgttcg gcccggtgaa
     3481 tgtggaaatc gtcaaacgcg gtttgaactc gggattcaaa caggcttttg ttaccggatt
     3541 gggcgccatg acggccgaca caacgtatct gacaatcatc tttttcggtc tcgtttcgtt
     3601 tttgaagatg cctttgttga aaatattttt tgccctcgcc ggcagtctga ttttgattta
     3661 tctcggtgta accagcataa aagatttcct gggcaaaacg gactatgatg aaaaacagaa
     3721 tcggcggttt tttaaaaatc cctacgtcgc cggttacgtg attgcgcttt cttgtccgat
     3781 caccatcgtc tggtggacgg gtgttttcgg cgccctgctg gcatctcagg acagcataca
     3841 aacaaatttt tccgcatatc tttcgtgtct cactatattg ctcggctgct tcatctggat
     3901 cgtttttctc tccgccgcgc tgcactgggg gagaaaaatc gtcaatgaaa aatttatcgg
     3961 atttgtttcg ttgatcgccg gcatgtgtct ggcgggattc ggcttggctt ttctatgccg
     4021 gtccgtgcat ctgatcatac attgacagcc cgggatcagg cccgggctgc cggattgaac
     4081 tatttattgg aaagatactc gtgaatcgcg ttggccgcct tgcgccctgc gcccatcgcg
     4141 agaataaccg ttgcggaacc ggtgacaata tcgcctccgg cataaatgcc ttcgcggctg
     4201 gtcagccccg attcatcctt ggttagtata tatcctttct tattcgtttc caaaccggtg
     4261 gtggttgcgg ggacaatcgg attggccatg gtgccgacgg caacgacaac ggtatccaca
     4321 tcaatcacga attcggatcc ttgaatggga acggggcttc gtcttccgga cgcatccggc
     4381 tccccaagtt ccatttttat gcacttcatc ccagtgaccc agccggcttc gttgccgata
     4441 tattccaccg gattgtgcag caggaggaat tcaacgccct cttctcgcgc gtggtgaact
     4501 tcttctttac gcgcaggcat ttccacttcg gtacggcggt aaataatcat cgccctttcc
     4561 gcgccgagtc tcaaggccgt acgcacggaa tccatcgcga cattgcctcc gccgatcacg
     4621 gcgaccttct tgccgcgaac aatcggagtg tcgtattcgg gaaacaaata ggccttcatc
     4681 agattggagc gcgtgagata ttcattcgcc gaatagatgc cgctcagatt ctcgccgggg
     4741 atgttcataa agaccggagc gcctgcgccc accccgatga aaacggcatc aaatccatcg
     4801 gcaaaaagtt catcgattgt tttcgtgcgg ccgatgacaa aattcgtttc tatcttaacg
     4861 cccatttttt tgagatagtc cacttccgct tccacaatgg ccttcggcaa acgaaactcc
     4921 ggaatgccgt aaaccagcac gccgccggcc ttatgcagag cttcaaagat ggtgacatcg
     4981 taacccagtt taaccagatc gcccgcaatg gttaatcccg ctggaccggc gccggcaacg
     5041 gcaatttttt tgccgtttga ttgcacggcc ggaggaatgg aaattttgcc gctgttgcgc
     5101 tcatagtccg ccgcgaaacg ctccaaccgc ccgatggcca cgggctcgcc tttcttgccg
     5161 agaatacatt ccttttcgca ctgatcttcc tgcgggcaga cgcggccgca gacagcgggc
     5221 agaccgttgg tttcttttaa tttcaaggcg gcgtcaatga aacgaccttc cgcgatggcc
     5281 ttgataaact cgggaatctt gacatccacg ggacagccgc tgacgcagcc gggtttctta
     5341 cactggatgc agcgctgcgc ctcgataatc gccgtttctt cggaatagcc gaaaggcact
     5401 tcttcaaagt tgtgaatcct ctctttgggg tcctgttccg gcatggtctg ccggggcact
     5461 ttttctttct ttggcttcgt ttctttagtt tccatcgcac ccacacttat gtttttcata
     5521 ggcttcccgt tcctgatcac aatacatcct caggcggtcg gccagtaatt taaaatcaac
     5581 ctggtgacca tcaaattcag gaccgtccac acaggtaaac ctggtttttc cacccacatc
     5641 aacacggcag gctccgcaca ttcccgtagc atcgaccatg atcgaattaa gactgacgat
     5701 ggttttaatg ccgtgcggct ttgtcacatc gcaaatgaca cgcatcatgg gcacggggcc
     5761 aatggcaaac acacgatcga tcttttcgcc cttgtcaatc atctcctgaa gaacattgct
     5821 gacaaaaccg tgaacgccgt aacttccgtc atcggtggtg accaccagac gatcggaatt
     5881 cgctttgatc tcttcttcca gaatcagcag gttcttcgtg cgtgacccga tgatggaaac
     5941 aacctcattg cccgcttttt tcagggcgac aataagcgga taaacgacgc cgacaccgac
     6001 accgccgccg atgcaaacga cacggccaaa attttcaatt tccgtcggat ttcccaaggg
     6061 accctgcaca tcctgaatcc catctcccgg agccattgcc gcaagcaacg ccgttgtctt
     6121 gcccacaact tgaaaaataa tggtgatcgt tcccttctgc gcatcagaat cgacaatggt
     6181 cagaggaata cgttcccctt tttcatgaat cttaagaacg ataaactgtc cggctttccg
     6241 cttttgcgcc acgaccggag cctccagaac cattctgacc acgctctcgg ataaattttc
     6301 cttttcaata attttattca agcttaattt ctcctccatg aaaaaaacag gtgttcgtct
     6361 ttattaaatt ttaacgtgaa aaaatactta aggtaaaaaa aacagcacca cctgccaacc
     6421 attccatgat tgaacagttc ttcgtatcgc agattctttg ctgaaacgtt ttttaatatt
     6481 ccggtgtcag tttgtccagt tccgccagag aaaataccgg accgtctttg caaacatatt
     6541 tactgcccac gttgcagcgt ccgcatttgc cgatgccgca tttcattctc atttccaggg
     6601 acgtgagaat gttttgcttg gaaaagccca gatcgaaaaa taccggaagg gtgaaacgga
     6661 tcatgaccgg aggcccgcag ataacagcga cagcgttttc tttactgggc gcgacctctt
     6721 tgcataccgc agggacaaaa ccttctttgc ccttccaagt ggcgtcgcct ttgtcaaccg
     6781 tgacattcaa attgatatcc gctcttttac cccattcgac cagttcatct ttataaagca
     6841 gcagtccggg atttcgtgcg ccgtaaatca ccgtgatatt tccgaagcgt ttgcggttgt
     6901 cttcaaaaag catgtaattg atgagcgacc gcagagtggt gaaggcgaaa ccgccgccga
     6961 cgataacgat atttttgcct gccagaaaat ccagcggcca ggaattgccc aaaggaccac
     7021 gaatacccat cacggttccc tcttccattt cgtgaagcgc cgaggtgacc acgccggctt
     7081 tctgtaccgt gaattccacg taccctttct gtgttggaga cgaggcaatg ccaatcggcg
     7141 attcgccttt tccgaaaatg gaaagttcgc cgaattgccc gggaatgtaa gcatatttct
     7201 gttcgtcttc cggattcacg aatttaaaac ggaatgtttt aatgtctttg gtgtcgactt
     7261 cggtaatgat tttatccacc acaacgggat acggtatata cgggttctgc attactaact
     7321 ccctgattat aattttgaaa tatcttcaac aattttacga atatcaatat tcaccggaca
     7381 gctcatgacg caacggccgc agccgacaca ggcaatgcaa tcgaacatgt ccacgtagta
     7441 cttaaactta tgcatcgcgc gctgtcgcca tctttccttc tgcgaggagc gcgggttgtg
     7501 tcccgatgtt tccttggtaa acagcgggaa catacaggaa tcccacgacc ggatgcgaac
     7561 gccgtcgctg cccttgactt catcgctgat atcgaagcag tgacaggtgg ggcacatata
     7621 ggtgcaagtt ccgcaggcaa gacattttcg gtgaatcgta tcccagaagg gattgtcgaa
     7681 atttttatcc agaatcggct tgatttcatg agcggggatt ttggacgtaa tcgcttccct
     7741 ggctttcgcc gcaatttcgt ttttcttggt ttccgttgcg gcgtccgcct tggcatcgcc
     7801 gaagtaggaa gccagttttt ctcccttggg cgtgatgaac tcgaccagat aatcggagcc
     7861 gatgtccgta atcagaatgt ccgcgccatc cgaagaaacg ggttcgccgt caacggatgt
     7921 gcaaaaacag ctggcgtagg gtggtttcac gcaggccaga gaaataaccg ttgtgctctg
     7981 tcttttggcg atgtagtagc cgtccttgta tttttcctga tcaaaaagtt tgtccagcag
     8041 gacaaaacta tgggcgtcac agggacgaac gccgaacaaa actgattcga ccgccttatt
     8101 ttcttctgtc gagaaaacca ttcccttggg tgtccgggtg tatttcatca tggtttccgt
     8161 gtgcggaaag aaaacatttt ttggcgcgtt cttggaattc aaataagcaa agaggggttc
     8221 ggcgtttttc tgcaaaggtt caaaaagcac attgtcttct tttttcaccg gcgcccagac
     8281 aagaaacttc tcggtcattt ttttggcaat acccgccagc gcatcttttt taattaaacg
     8341 tttttccata tttttcccta attaagaatt taatgagacc caagcttcag aagtgtagcg
     8401 cactgaagct tgctggtcga attatccaaa cgcatgaagc ttagcgggat aatatcaata
     8461 ttcatccgtt ttcatcacct ataattaaaa tcattcgctt acaatttatt aaatcagccc
     8521 caactgcgac aacagcggcc ggggatcaaa aatatgcgat ttgaacgtat ctttcgatcc
     8581 gggaagcccc attgccaacg atatcaattc cgtgaaataa aatacaggca gtttctttcc
     8641 ttcgctggga cgcatatcca gattggccat acacagcgga cacgccgtga cgacgcagtt
     8701 cgcatccgct tcacgggcgc cgttcatcaa ccggtcacac atttccacca caatcgcggt
     8761 tttactgatc gccaatgaac cgccgcagca atcggtctta tacgaccagt tcttaacatc
     8821 cgcgccgatg gctttcatca ttttgtccag cataaacgga ttttcgtaat tttcgaactc
     8881 acaaacctcg ggcggtctaa gcagcaggca cccgtaatag gaaacaggtt tcaatccggt
     8941 cagaggttta accaccttct cggcaagcac gtcgatacct atatcgttga aaatgatgtc
     9001 gatgggatta cggatggcaa tgccgccctg atacttcgcg ccgacgatac cttcaatttt
     9061 cgccttgaga tccttatcgg cttttaaatg gtgttcggct gttttaaaac ggttgaaaca
     9121 ggcggcacag ggtaccatga catccatcgc atctttttct gcggcaatca ggtttcgcgc
     9181 cggcagagca atggacaaat caaaatttgt attgtgcgcg gacgatgcgc cgcagcaaca
     9241 ccagtcatca acttctttga gttcgatgtc gagggctcga ctgacggcct tcatcgactg
     9301 atcatactct tttcccgtcg cgtgcagcga gcagcccgga taatatgaaa ctttcaagag
     9361 tagcctcctt aaccttaaac ctctttatct gttttttgtt ttttcaaaaa tatttctaac
     9421 cgatttcatg tctttcgtgc ggggtgacaa aagggccagt tttcctttct taaacatatc
     9481 cagacccagc agcgcatcgg agaataaatc tccggacttg agcttgtact gaaccatcat
     9541 catcggttcg ttgatgcgtc cgccgaattt catgccggcc aggaatgcct catggaactt
     9601 caaaacattc gcttcgcgga cagtgccctg ctgtatggcc atctgcctga gcacatccat
     9661 catactggca atatccacct gattggggca gcgggtaata caggtttcac aggacaggca
     9721 aagccagatg gtggaactgt tgagcacctt gtctcgctgg ccgttctgaa tcatcttgat
     9781 gatcttgttg gggttgtact ccatgtgctt tgtcatggga cagccggccg tgcatttgcg
     9841 gcaatgatag cagcgttgca gcggcacccc gtttatcttt ttgtttactt cttccagaaa
     9901 agtttccatg caaactccat tatctcaagc taattatggg ttgtagacaa agtctccctt
     9961 gtcgtgatgt tcatcgaatt tgcccagagg cggcatatcg tccatactgg atcctgcgcg
    10021 gttgccgaag aattcaaagc aatccttgtc gagtttcttg aggaacttcc ttaaatccac
    10081 gcccatcgga cagacggaaa tgcacgaccc gcaatcaaca cagcgtccga ccatgtgata
    10141 caatctcata aactggaaaa cctgtgtatc ggaagcgtct tcaccgatgc ccacccactg
    10201 cggctgactt tgttcgacaa aacaggtcgg acaatagcag gaaggacagg cctgacggca
    10261 ggcataacag cggatgcatt tctccatttc cttgttaaaa tacgcccagc gctcctcaat
    10321 ggatttgcct tcgaattctt tgacgtcgtc atattcggcg tcgggattca tcgctggagc
    10381 gggagaaccg atcatcacat cggaaataat gggattgttg aaccggcagg tcttgcagtt
    10441 atccgccaga acatccttga gcgcaaccgt tttatcaccg ccggaggttt tcatcttgat
    10501 ctggccgccg tcaatcgatc cgtccacgat atccgcgccg ccgatggcga ccttcaatcc
    10561 tttgccgtcc acatatccat cgcagggaac gccgatgata tacacttcat cacgtccgta
    10621 ctgtctttcc tgaagatgaa tgatcagaga acgagtggtg caaccgcgcg ccacgacccc
    10681 gacgattttt ttcttccggt cttccggttt gactcgtttt tgcgaatttt tatgctgcgt
    10741 aatcaggtcg tgcacatatt tggccagatt ctgtgtgcag aggttgttcc agaccagttt
    10801 attggtttct tccggcttgg tgatgaaaca aggcgttgcc gttaacggta aggtgccctg
    10861 ttcataacca acaatgacat caaccttttt ttccagaagc agcctgtttg cttcttcccg
    10921 taattttgtt tcgatgtttt ccacagtaat tgtttccccg ttattttttt attgcaaaaa
    10981 tttaatcaat cttttttatc aatttattcg ccggtcccag cgccttgatt ttctccgtag
    11041 cgcctttgat gacctgcgcc catctgtcac cttcggaggc ggaaacccac gtaaagatgg
    11101 ttctgtcacc ttccacaccg atgtagttca ggaaactttt taacgtcgca aacttgcgcc
    11161 gagccaccag atttcccgag atatagtggc attcaccggg gtgtcaaccc gacaccagaa
    11221 cgccgtctgc gccggcctgc agtgctttca cgatataaaa gggattgatt cgtcccgtgc
    11281 agggtacacg gatgatccgg acatttgtcg gatactgaat tctgctgata cccgccagat
    11341 cggcgcccgc gtaggtgcac cagttgcaga cgatggcaac gaatttcggt tgccattctt
    11401 tggttgctaa attttcagcc atatattctc cttccaacgg cctgacggtt tttagccgtt
    11461 tagccttcgt ttaaattata tatctgcgcc aatacttctt cgttgttgaa accgttgagg
    11521 tccacggcat tcatgcggca tgacgccgtg caaacgccgc accccttaca gagcaccgcg
    11581 ttgacttcgg cgcagccttc cgccacgttc acgctgatcg cgctgtaggg acagttgatt
    11641 tcacacaaac cgcaggcgga acatctttcc ttgttcacga aggcggtctt gccttccgct
    11701 tcgatatagt ccagcgtcag aatcatgcag gcgcgcgata cggcggcgtt ggcctgggtg
    11761 acggtttcgt cgctgaattt cggtgaatga gacataccgc acatgaacac accgtccgtc
    11821 gcgaaatcaa cgggacgaag tttgacgtgc gcttccatga agaaaccgtc ctgattggtc
    11881 ggcactttca gcatattgcc gatggccgcg ttctccggat tcggaacgac acccacactc
    11941 aaggccacca gatcagcagg cagactgacc tgttcattga gaatcggatc aaaaaccttt
    12001 acttctgcct tgtctccggc ggctaccact tccggtttgc ggtcttcttc gtaacagatg
    12061 aacttgatat tcgcttcacg cgccttcttg taataatctt ccttgaacgc gaaggtgcgg
    12121 atatcgcggt acagaatggt aatgtcgcgt gatggatcgg cttctttgag ttcgagggca
    12181 tttttaatgg cttcggaaca gcagtaacgg ctgcaatacg gtctttcctt gtcacggcta
    12241 cccacacact gaatcatgac cacatttttc acagacgcga gcttggcatc tttttcataa
    12301 atcatggtct caagttcacg ctgggttttc actgccgcgt tctggccgta gagatattcc
    12361 ttgggtttgt tttcataggc gccgatggca acaatcacga caccgtgttc aaacactttt
    12421 tctccgtctt tggctttgac cgtcgtctta taattgccga tgaaaccttc aatcttctca
    12481 attttcgcgc cggtggtcac cgtgatcaac ggattgttgt acactttatc gagaaggcct
    12541 ttcagatgag cccgtgtatc gagaccttcg agtgttttgt aaagatgatt gtaattaccg
    12601 cccagacggt cctctttttc cactatgaag gacgcaaatc cctgatcggc cagcgacaga
    12661 gccgcggtca taccggccaa accgccgccg ataatcagcg cctgatgatt gacggatagc
    12721 tgaccgggtt tcaacggctt gagatactgg gccttggcga cagccatgcg cagcagatcc
    12781 ttggcttttt ccgtggccgc ttccggttcg tgcatatgca cccaggagtt ctggtcgcgg
    12841 atgttggcca tctcgaagag ataacggttc aaaccggctt tttcgcagtt ttcctggaac
    12901 aatccttcat gcgttcttgg cgaacaggat gccacaacca cgcgggtcag attctgttcc
    12961 tcgatgacct ctttcatttt caccgccgta tcctgcgagc aggtgaagag attgtcggcc
    13021 gtataaacaa cggagggcag cgtggcggca taatcccgca cagcgggaac gtcaacgaca
    13081 ccgccgatgt tgataccgca atggcagaca aatacaccgg tgcgcacgcc ctgtccgcgc
    13141 atatcctttt cttccggatt ctgcacgggt gtaatcatcg taccgcgacg ggaagcaagc
    13201 aggccttccg cgcaggccgc ggcggcagag gcttccataa cggtttcagg aatatccttg
    13261 ggaccgccga aggcaccgca gacaaacacg ccggggcggg atgtctgaac cggattctcc
    13321 agcgatgttt tgcagaagcc atgctcttcc agttcaacgc cgaggctggc ggccagttgc
    13381 ttggcttcct tgggaggcat gaggccgacc gacagaacga ccatatcgaa ttcttcttcg
    13441 gtcagggttc cgtcctgatt gacataggta atcagcagat tgttggtctg ctgcaattct
    13501 ttgatggtgg acggcatgga acgaatgtag cggatgccat attcgtcttt ggcgcgattg
    13561 acaaaccggt cgaaatcttt gccgtgcgca cggatgtcca tgtaaaagat ggtgggttcc
    13621 agtcctttgg cgtgttcctt tccgataatc gcctctttgg tggcatacat acagcaaacc
    13681 gatgagcacc agctgtttcc acaggacgta tcgcgcgacc ccacgcattg aatgaaggcg
    13741 attttcttgg gctccttgcc gtcggaaatt ctctggacgt gaccgaagaa cggaccggac
    13801 gcagaaagga tacgttcaaa ttgaagcgcg gtaaccacgt ttttgtaaat gccgtaaccg
    13861 tattcaccgc gcttgctggc gtcgaaaatt tcaaaaccgg ccgaggcgat aatggcgccg
    13921 acttcaatgg tttcctcttc cactttcatg tcgtgatcga tggccttcgc aatacaggcc
    13981 tccacgcact gataacattc ggaacagaca ccgcaggaca gacagcggct cgcttcggca
    14041 atcgcttgcg cctcactgaa tcccagaccg acttctttga aattggtttt gcgatcctcc
    14101 ggtttcatca gcggatattt ttcacgggga actttggaga aattggaaag atcggctttg
    14161 tccgccagat ccttggtcca gtctttttca cggccggctt tcatatcctc accgcgcaga
    14221 taacgatcga tggatttggc cgcttcctta ccggaaaaaa cagccttgac gacggtctga
    14281 ggaccggtag aaacgtcacc accggtgaat atgccgggaa cgttggtcgc gtaagtgatt
    14341 ttatcgaaat ctacattatt ccatttattg atcgagacac cgctttcctt agtgatgaac
    14401 gacaaatcgg cttcctgacc gatggcggga acgatggcgt cgcattcgac gataaattcg
    14461 gagcccttga tttccacggg acgacgacgt ccactggcgt cgggttcacc cagttccatg
    14521 cgcgtacatt cgatgccggt cacttttcca tcctggccca caacgcgttt cggagccacg
    14581 aggaatttca tctcgattcc ttcttcaata gcctcctcga tttcttccgg agacgcgggc
    14641 atttccgcac gggtgcgacg atagaggatg aagacttctt tcgagccggt tctcaccgcg
    14701 gtgcgcacgg cgtccatcgc gacgttaccg ccgccgatga cggccacttt gtcgcccagg
    14761 gaaacttttt cgcccaggtt tacttttaac agataatcaa cgccgtgaac cacgcccttc
    14821 atatcctcgc cgggaatgtt gagcttgctg ctcttcatcg taccgcagcc gatgaaaatt
    14881 gcgctgtaat cagacttgag tttatcaaag gcaatatctt tgccgattct ggtattgagg
    14941 tgaatcttca cacccagatc ttcaatgatc ttgatttccg ccttcagaac attcttgggc
    15001 aggcggtatt caggaatacc aacggcgagc cagccgcccg ctacgggcag ggcttcgaaa
    15061 acatccacat cgtagccttc aatggccaga taataagcgc aggttaaacc agagggaccg
    15121 gcgccgacga cagcgacctt cttgcccttg ctttctttct tttccggaat atagcgggta
    15181 tcgctgttga gatccaggtc agcgacaaat tgtttgaggt gcatgatatc gatcggatca
    15241 tcgacttttc cgcgcataca ggccgtctcg cagggatggt tacagacgcg tccgcaaacg
    15301 atcgggaaag gattatcctg tttgatgagc ttgagcgctt ccttgaattt gccttcggca
    15361 atcagcgcca catacccctg aacactgatg tgtgtggggc aataggcctt acagggggat
    15421 gtccccacct tatcgattgc aaaaccgctg ggaatggcct gcgggaagtg acgatagatg
    15481 gcttttcgtt cgctcaaatt gccgttaaat ccggcaggaa ccgatacggg acaaactttg
    15541 gcgcagtcgc cgcagcccgt acatttatcg agattgacat accgcggagc ggatgtcatt
    15601 ttcactttga attttccggg ttcgccctct accgaattca cggtccgccg ggtcttgatt
    15661 tcaacattgg gatggcggcc gacttcgacc agtttgggag acaatataca ggcggaacag
    15721 tcattggtcg ggaaggtctt gtccaactga gccatgacac cgccgatgct gattccgttt
    15781 tcgacgagat agactttcat tccggaattg gccaaatcga gagacgcctg aatgccggcg
    15841 attcccgaac ccactaccat tacagcacca actttttcgt tatctttcaa cacctgatta
    15901 cacctcatta ttctgtatta aagaacttaa aaactttatt ttaatgccgc gccgctaggc
    15961 gttttttcgc taacacaaat tcaatacact gtcaagagca caagctgaat ttatttgatt
    16021 ttttatgcta actcattgat ataaaaagac taaaaaaatg accggcgttc actgaccggc
    16081 attcaaaaat aaatagtatt tcttggtcat gaatgtttgt caagagccct taaatgattt
    16141 tccagaaatt cgaggatctc aactttccct ttttgtgtct ttgccgaaaa caaaattgtc
    16201 tttttttcac ttgatatccg caacattttt tctatcgcgc gtttgcgcgc tatcgcctgg
    16261 ttgttggata atttgtcgga cttggtgaga atatataaat aaggaatgcg gtaatgatcc
    16321 agccagtcgc gcaacgataa atcatcggca ttgggatccc gccggatatc cagaatgaaa
    16381 atcacgagac aaagagattg cctctcccga agataggcct ccatcatatc cccccagtct
    16441 tttttgacgg accggggaac tttggcaaaa ccgtagccgg gcaaatccac aaaagatatt
    16501 gtttcgttga cggtaaaaaa attgattaac tgcgtccgtc ccggcgtgtt actggttttc
    16561 gccagatttt tgcggccgac gagcgtgttc atcagcgacg atttaccgac attggaacgg
    16621 ccgacaaatg cgatctccgg catgattgcc ggaggatact gcggcggcca cacggcactt
    16681 tttataaatt gagcactggt gattttcata attatctgac gcaatacctt gtcttaatct
    16741 atgcgttcta ttttctcatt gaccttaaga ttctcttttc tttcaagact aaaaaaggaa
    16801 aggtcaacag cgttaaagcg taatatataa cgattccctt taaataacat ccattttccc
    16861 cctgtcgttc cataatcggc ttgtaatatt tcttaaaaaa atatgctgtg taatacaacg
    16921 accagaaaag tttagcaaga gctccgacgc gttgcccacc ggtttgaact tccgaagagt
    16981 gcatatgcac actgtgcgta tcattggtta taataatctt tccgccgttt atgtgtattt
    17041 tcgcctgaat gtcgttttcc tcacggaatc cgttgccccg gctgtaaaaa gtatccatct
    17101 cgtacgataa taaaagctct cttctgacca gataaatggc gtgggtaaaa ggaagttcta
    17161 catcgctttt aaacttcgca tcggtattga tatagaaaag ccggaaatca aaaggcggca
    17221 cgtcaatact cccctgtccg aaacgggcaa tcgcttcagc cacattctcg cccgcattgc
    17281 ggaaaaaatg gcgtcccgaa gcaatatccg ccggcagact tttcagttta ttaagacaaa
    17341 ctgtcgtgta attttcttcc agaaaatcgt cgtcatcacc gaaaagaaca aactcgccgt
    17401 gagctgccag aagtccccgc cggcgtgaat agggagcgcc ctgccttttt tcatttttca
    17461 gatacattgt ttttattcga ggatatttcc ggacgaattc atcaaccacg acagccgttt
    17521 tgtccgagcc tccgtcgtca acaatgatca gctctgcaac aaactgctgc cggtaatacg
    17581 aatccagaac ctgagcaagg gtgtaccccc ggttgaaggt gggaatgata acgctgatca
    17641 taaaagtatc agtacccttc ccgttacttg tcatctcatt tcataaatac gtacaggtca
    17701 agacgctcta tcctaaatac ttagacactg cacgttcatt atttattatc caacacagtt
    17761 aaatctcggg agatcgatca tacggatttt atccctactt cgttcagaaa ggaaatccgg
    17821 aggcgaccga tgagttttat gggctgttta aaagcgccgg gatgctaatg ctcaggtatc
    17881 gcggattaag tcctgctttt gtcgcatgtc gctacgatat gagtacagcg caataaagca
    17941 gaccaccgcg ccctttagga ggtatgcatt tttcaacagc ccatcattcc cgttcaaaag
    18001 tgatgccgta tttttccagt ttccgttcca gcgtcggacg ggagaccccc aggatttcgc
    18061 aaatctcacc tttattctta tccgtctctt tgataacttt gcgaatatag cgttcttcca
    18121 cttcgtcgag tgtaagcaac ttgcccggct cggacgcttg aaacaaattc gcgtcacggt
    18181 caccggccgg cccggcagct tcttccttgc ccaactcggg aaaatcgttg actcccagca
    18241 cctgcccctt ggcaacaacg caggcgcgga caagcagatt ttccagttca cggacatttc
    18301 ccggccaggt gtattgcata aatatttcca tcacttcatc ggaaacaccg atgattttct
    18361 tgtggagatc cagattaatt ttggtcagca ggtaatcgat caggggcgga atatcctgac
    18421 ggcgggcgcg cagcggcggc agcgtaatgg aaacaatgtt caaccggtaa tacaaatcat
    18481 cgcggaattt gccttccttg accagcgcct tcaaatcctt gttggtcgcg gccataatcc
    18541 gtgcgttgac cttgactttt tcttttcccc cgactctgtc aaactccatt tcctgcagaa
    18601 cacgcaaaag tttcgcctgc aggttcacgg acatctcgct gatctcatcg agaaagaccg
    18661 aaccaaaacg ggcgagttcg aatttgccca atttgcgggc cacggcgccg gtaaacgatc
    18721 ctttttcatg accgaacaat tccgattcca gaagcgtttc aacaatagcg gaacagttca
    18781 cggcaataaa aggttcgtcg ggcgacgtat tgttatgaac gactttggcg attaattctt
    18841 ttccggtccc gctttccccc tgaatcagta cggtggtgcg gctctgggaa accgtgccga
    18901 tgattttaaa tatttcccgc atctcgttgc cggtgccgat gatatcgccc acacgaaagc
    18961 tgcgggatgg ttccatcaga agcccgtcta tcttcttttc catttccagg gatttcaggg
    19021 cttttttaat cgccagatcc agttcgtcga cgttgacagg tttatgaata taatcgaaag
    19081 cgccgctttt catcgcctgg a
//
LOCUS       DAOOLB010000035        19000 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_2968_length_19000_cov_35.893903, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000035 DAOOLB010000000
VERSION     DAOOLB010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 19000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19000
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2968_length_19000_cov_35.893903"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(13..237)
                     /locus_tag="PLF77_06010"
     CDS             complement(13..237)
                     /locus_tag="PLF77_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08442.1"
                     /translation="MKTNLTDHQAKTAYLEQHYGKDILPLSAFEQTMPGSEPIEILFF
                     YKKDTPVSKLRASLLKTHGRRLGRRSAVSG"
     gene            complement(688..1170)
                     /locus_tag="PLF77_06015"
     CDS             complement(688..1170)
                     /locus_tag="PLF77_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08443.1"
                     /translation="MKLHSLLLLIILAAIAAFSALNWSTFISPTNLSLGFTSVHMPLG
                     LVMLGMLVLIVMLCLMFVVYIQAKALLESHQHAKALKAQRELADQAELSRFTELRNYL
                     EGQLRAQEAKSSELKTEILARIEQQENHLRTAIEEAGNTLAAYIGEMDDRLGKKTDGG
                     "
     gene            1423..1920
                     /locus_tag="PLF77_06020"
     CDS             1423..1920
                     /locus_tag="PLF77_06020"
                     /inference="COORDINATES: protein motif:HMM:NF018232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 family protein"
                     /protein_id="HPC08444.1"
                     /translation="MDKIFTKKGVLFGFLLVAFIIVFEIILASLSLPAWPAFMVMIFF
                     FVVHEDTKQGAPIVIGGLAGIFCAVLSEMFIDVLMPVMGHEAAKLLFIGLFVYAIVLL
                     KDAIPYVFNSYAFMFFLVSAIARPSATVSPFVWMAVELVVGVIFILGILGINRIVSAI
                     LTEEK"
     gene            1933..2355
                     /locus_tag="PLF77_06025"
     CDS             1933..2355
                     /locus_tag="PLF77_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPC08445.1"
                     /translation="MMIVTKTIKLQTQGFNDMVNITPRVLSLLEDSPVADGLICVFCP
                     GSTGSVTTIEYESGVLHDLKKALERIAPSDIPYRHDERWGDGNGFSHVRAALMKPSLT
                     IPLVGGRPTLGTWQQIVFIDFDNRKRHRELVVQIIGDA"
     gene            2556..4094
                     /locus_tag="PLF77_06030"
     CDS             2556..4094
                     /locus_tag="PLF77_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPC08446.1"
                     /translation="MKKVVIAGVVIIVLVFSFIGFLHGNNAKPSSPSGAAVSSGMPSG
                     ADTYMLLKNVKFRFAEDIFINVHHMLSITEKIKPYPYVDMNNDQSFSLKIISGHCSLE
                     PPVMETLFNKHVLNYKGAPLKDLKLSTVEMDIDGQKVPMLKTSGMMKLVVWLPFEMIA
                     RVGVDKPTNRMTLEAYKIKSVGLPFVKNLMDMSGVKLSTLLSIEPGRGLSIEKNTMYV
                     DALNITPPPKLVGKLLDAKANVSTNSVDLTLGEEKPANVKYTLLVPDAKNFIYIFQGS
                     LIFGKLLALDANLQLVDANPADYLDFYLKKYLLQLSRSAIRMKPDASLIVNIADYQSD
                     SSPAPAKPAEPAKPTSLSKAAPAKAVSTKPVAGKPAKTPAAKPAASAPAVKQHASINL
                     HLEFDTAQSAVKEQYHSHIKKLADLMKKSPDSRVVIQGHTDNLDKFNNPDNNIKLSQA
                     RADSVRQYLIDKFGINAARIKAIGYGPNQPIADNATLEGQKKNRRVEAVIQTSPQGMV
                     ASTL"
     gene            4280..4837
                     /locus_tag="PLF77_06035"
     CDS             4280..4837
                     /locus_tag="PLF77_06035"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HPC08447.1"
                     /translation="MIRLEKIISGGQTGADRAGLDFAIDHKMDYGGWVPKGRKTEDGS
                     LPLKYHLQEMPTRDYAMRTLQNVLDSDGTVIVSHGFLTGGSALTREFALKYKKSWLHI
                     DLNALSLPDAAHMLLHWLEEKEIRVLNIAGPKAGKDPKIYKAVLRLLQEALTDSHVLG
                     SGKKSCVKLQCTPSSKPGSSGEKQK"
     gene            4906..5601
                     /locus_tag="PLF77_06040"
     CDS             4906..5601
                     /locus_tag="PLF77_06040"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC08448.1"
                     /translation="MTNPNTLLIRLLTRLRDMRLVWDIMGSLYNGRIYNLIEELYDDI
                     AKDLDTPDQAQILDAGAGRGYLSVALASRNPDSHVTGVDYSRMQVRRAERYRREKKIP
                     NCSFRQNNVLSLDFQNAAFDAAVSVGSIKHWPDSRRGLAELYRVCKPGSCLIISETDR
                     DVPDNELRKFVQRFRLPFVPEDLLFWGLRHVVFGQSFSQDQLADAVRLAGFRNIESHK
                     VKACPYVIVKAWK"
     gene            5729..6640
                     /locus_tag="PLF77_06045"
     CDS             5729..6640
                     /locus_tag="PLF77_06045"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HPC08449.1"
                     /translation="MTLKGILTVVRIPFLILALILGILGAAVAWYESVRYGSPFNLCY
                     AVLATIGLLIAHAAVNIFNDYFDARSGLDYKTNRTPFSGGSGAVPTGLLTVHESLQLG
                     IICFVVLVPIAVFFIMKSGWMLLPLLIAAMLLIVLYTPFILKMGYPEWSPGLGLGILP
                     VMGAYFVHTGQYAPTALVASMPSYFLVHNLLLLNEFPDAEADVTVKRRTLPIVAGKKN
                     AAFFFSGVTLLVYIWVLGAVIWGSMPVFTLVCFLTMPLALQVIRGSFRYDERELLMDT
                     MKKNVLLVLLTQALIAAGYIAAGIFSR"
     gene            complement(6668..7123)
                     /locus_tag="PLF77_06050"
     CDS             complement(6668..7123)
                     /locus_tag="PLF77_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08450.1"
                     /translation="MENIFDKIKSNLKKGMEEGIAVLREGATVVSVRMNELSEEGKRQ
                     YKIFNLNLKIQEQIKELGCMAYAALDKGKRLDDDKKIKAAYSKIKKIEWQLTKTEGQK
                     KVKTPSAGKTAAKPGAKRAKASPKKAKPQSAKAAGKSSAPGTGPKATSG"
     gene            complement(7159..7845)
                     /locus_tag="PLF77_06055"
     CDS             complement(7159..7845)
                     /locus_tag="PLF77_06055"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HPC08451.1"
                     /translation="MERKKLTITIQQGENVSAVLTLPPDKKKHYRAGVIVAHGAGNDM
                     EHEMLVDFSDGLARAGYPSLRFNFPYKEKGLKAPDGQKKLEATWGAVFLHFQTDMPFQ
                     VDHVIASGKSMGGRVASQMAAEKQLPADGLIFLGYPLHPAGDLTKTRDAHLYRLDVPL
                     LFFAGTKDALCDMILFRKVLKNLSAPWQLDVIEGGDHSFHVPKSLGIRQSAIHDRIVK
                     TSGAWLDGQF"
     gene            complement(7879..8457)
                     /locus_tag="PLF77_06060"
     CDS             complement(7879..8457)
                     /locus_tag="PLF77_06060"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPC08452.1"
                     /translation="MMENKKDSIPDLAECEDLMVRYAMLPNIVEHSRQVMRVSLAITD
                     HLQEGLSINRDLIVAAALLHDITKTRSLQTGEKHADSGGVLLRKLGFRRVAEIVEQHV
                     TIPDLNLQGGLEEREIVYYADKRVMHNVIVGLDERVDDLIKRYATDEKIRRRILLNKN
                     QALAVERKISGFMKMDIHQAIGKLVAHDRDKP"
     gene            8815..9699
                     /locus_tag="PLF77_06065"
     CDS             8815..9699
                     /locus_tag="PLF77_06065"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPC08453.1"
                     /translation="MRRTLVGFALSFFSALLLFTFPANTGALSKADVSFSSDTLDRGG
                     VILVTVKTNEQKKPRITWLQKKVPLVYSTSDESWSGFISADLHQETGLYDCMIRETSS
                     GFQERYPIRIRLKDYGVRRLKLPKTKVELDAHSLKRVKNEAAVIHSLWPAGDTSPKWK
                     GHFVMPVDGDIVGTFGKKSIINQLHRSPHTGVDLAGKKGDPVVAINHGRVLLAADHFF
                     SGKSVYVNHGGGIISMYFHLDKILVNKGDDIHKGEVIGLLGATGRVTGPHLHWGVRIN
                     GNRVDPLTLIALSGTLEE"
     gene            9767..11032
                     /locus_tag="PLF77_06070"
     CDS             9767..11032
                     /locus_tag="PLF77_06070"
                     /inference="COORDINATES: protein motif:HMM:NF013661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HPC08454.1"
                     /translation="MIFKKKTSHGFTFLISFIIVIFAVMFLATDVHALDISNMYSPLN
                     KKRPLRSKTQYIILHTTEGALNGSLRKVHQFGETHYFVTPTGKVYRIIDRKKIATHAG
                     RSMWNRQTTLDNVSIGIEVVGFHDQDITRAQYTALRQLLRQLKKIYRIKDKNILTHSM
                     VAYGRPNIFHPRKHRGRKKCGMIFSHPDVRKRLGLLPGPKRDPDVAAGRLVVADPDLH
                     RFLYKPPKVLAALKGTKDSSGSRSMVIAQGNNAWRIARDKYDAPSTIYTYPNGKKLRG
                     NEIRKWDSIPVGTRVSFVDEDREQEFEKFLEIGKDGNTVQDLAGNLYADPTTIYFFPD
                     GLIRTGAEMKKSSSLRKLLKNPPKGTRLLVGYIYGGYVQKGRSATRIAGRKWNYPSTF
                     YRMPDGRILNGDNVEKKSIPSGTLIFIMK"
     gene            complement(11166..11585)
                     /locus_tag="PLF77_06075"
     CDS             complement(11166..11585)
                     /locus_tag="PLF77_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014810270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPC08455.1"
                     /translation="MKSFRKELWFNTKKRREFINITSQVEDCLEESGIHEGIVLVNAM
                     HITASVFINDDESGLHQDYENWLENLAPHEPVAHYRHNRTGEDNGDAHLKRQIMGREV
                     VVAVTNGQLDFGPWEQIFYGEFDGRRRKRVLVKIIGE"
     gene            complement(11598..12020)
                     /locus_tag="PLF77_06080"
     CDS             complement(11598..12020)
                     /locus_tag="PLF77_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HPC08456.1"
                     /translation="MKFLRYIWALPNTTLGLLFVPLALFTRGGMHVVNGVLELHSRLI
                     SLILKKCMPVRGGVSAITLGQNRESLSINRRHEHAHVHVRQYEMLGPIFIPVSEKLIS
                     WLTENQIKVLNVAGPRLRKDPTIYDAVLRLLKVTLSAA"
     gene            complement(12069..13859)
                     /locus_tag="PLF77_06085"
     CDS             complement(12069..13859)
                     /locus_tag="PLF77_06085"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPC08457.1"
                     /translation="MTVYKRRLWLLCLVTGIVFHCVHAHALEPTFLKDSPKVNICRAL
                     EFLEDPAGELSLEQLLHPGTQDRFVPLNDPQLNFGITRSTYWFRFLLKFDQSENSCEY
                     ECLDNNSWLLEIDRAWMPTIELWVPVIRDSHDIRKTAQIMGESWRKLSAVTSDQSNRI
                     NILNRSSIFFIPKDYDESRPMYLKMASWGPPLNFSLHVRRLNEFRNWLTWDFTVFGII
                     YGLIIAMTLYNACMYLSLRDKTYLVYVLYIFSNFLYLSIGLGHFSALTGILSGSHFYL
                     IYVFTGSTGFFAAWFCRTFLLTSKNAPLIDKILMAYMFFAILLILMAVTGFYYQVQIL
                     GAAIGLTFPIMAITAGLVCFIKGMKPAGYFLTAWSVLSVGMLTWAFRSLGILPQTQLI
                     TYSYPIAVAAESILLSLALADRIRTLQQENRELENREHRLLILSNTDGLTKLYNKRYF
                     NEKLKSEISLSGNLGDPLSLLILDLDDFKKINDAYGHPAGDQVLKRLGEILLMSIRDK
                     DYGCRYGGEEFTVILPGAEKEKALDVAERIRRLISDDTFETSDGQKFSVTVSIGLAQL
                     RPGENSADLINRADQSLYSAKRQGKNQTVA"
     gene            complement(14114..14371)
                     /locus_tag="PLF77_06090"
     CDS             complement(14114..14371)
                     /locus_tag="PLF77_06090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08458.1"
                     /translation="MSEAFTRESIREHHRVKIKKIINQGGGVEEIDNYLMNRISTVIS
                     YERRKNERRQLRKVTKNRSVFSNDEALTEILYLAIRDVMKK"
     gene            complement(14858..15004)
                     /locus_tag="PLF77_06095"
     CDS             complement(14858..15004)
                     /locus_tag="PLF77_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08459.1"
                     /translation="MKSYRKELWFNTAHRREFINITGQVEDCLRESGIREGLALINTT
                     KEYF"
     gene            complement(14973..15611)
                     /locus_tag="PLF77_06100"
     CDS             complement(14973..15611)
                     /locus_tag="PLF77_06100"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPC08460.1"
                     /translation="MENKNCKNKRVHVVLFDFGGVLSEEGWKKGLRIIAKANGVHPDS
                     FLQTAADTIYETGYILGKGSQSDFWKSLKTKTGIRGDVASLSGELISRFALNDAMMEA
                     VRKLKSENFIVGILSDQTDWLDGLNARFDFFRHFDYVFNSYHLGKGKRDPSLFDDIAG
                     LLRETPENILFIDDDPGNIQRARQKGWKAILYTDFKSFREEINEIIQERTLV"
     gene            complement(16157..16765)
                     /locus_tag="PLF77_06105"
     CDS             complement(16157..16765)
                     /locus_tag="PLF77_06105"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF015875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016432 - tRNA-uridine
                     aminocarboxypropyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HPC08461.1"
                     /translation="MRSNRLPNHNTHCSRCYQRREICLCPIIPAVETSVEFLILRHIY
                     EAEKPSNTGRLAALALTNSKLISCGGGQRIGLACVDDDLLNAPGTWLLWPDGAGTLPR
                     TSDRQSPARVVVIDATWQQARRLYSSLPAFWRMPRLALEAPVAQRKRLRDQHCPNYMS
                     TLEAVAAAVAMLEGGGKNVPLDNLYDELVRRRRSLRWGSKES"
     gene            complement(17142..18602)
                     /locus_tag="PLF77_06110"
     CDS             complement(17142..18602)
                     /locus_tag="PLF77_06110"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPC08462.1"
                     /translation="MNSNLTTMWNAMQSGMGNHIPALLGALAVLVLGWLLAVLARAGV
                     RRGLGKLNLNTRFAQLTGQSINLENGLAIAIFWIVMLFIITAVLNSLDLAMVSAPFAA
                     LLTQFLAYIPHLLAGLMLALIAWLLASIVRRFVTKALSKTTLDEKLSEHAGMSPISDG
                     LSQALFWLIILLFVPAIMGALQMDGLLDPFRDMITKVLDFLPNAAAAVAIGFVGWILA
                     TVLRNLTTNLLRTAGSDKLSQKVGLAESVKLSNAIGLLVFVAVFLPALIAALDALKIE
                     AISKPAMDMLDMILMAVPHIVAAGLILVITWMVASFASRLLASLLNAMGFDNIPKHIA
                     LEHAFQKTPASVFVGYVVLFFAMLFATVEAANQLGFTHVSDTVDTFIRFGGDVLLGSL
                     ILVIGFWLANLAYEAMDKASGPNTKGLARIGRFAILGLVLALGLRAMGIADDIVNLAF
                     GLTLGAIAVTVALSFGLGGREPAGKLMEHWLSRFRQ"
BASE COUNT         4913 a         4782 c         4288 g         5017 t
ORIGIN      
        1 tcgtcgaatt ttttatccag agacggcaga acgacggcca aggcgccgtc cgtgcgtttt
       61 caaaaggctc gcgcgcagtt ttgatacggg cgtatctttc ttatagaaaa agagaatttc
      121 tatcggctcc gatcccggca tggtttgctc gaatgccgac agcggcagaa tgtctttgcc
      181 gtaatgctgt tccagatagg ccgtttttgc ctgatgatcg gtcaaattgg ttttcaaaag
      241 gttcaccccc gtcgggaaac tctatttttg aatatcgtca tcttaatccg aacctttatg
      301 acttctcggc atgaaggtat tttaaacata aacgaaactg aaatgcacaa caatttttag
      361 tcttgaataa attttttaaa ataattataa taactaaaat tttcacaatt aataaagaag
      421 aactatagaa attttagaaa gcggtccccc tgcaggcgga acgtaaattc acgcacacac
      481 ggagatttaa attccaatct cattcaagat caaaggagaa aagacatgat aattccagaa
      541 tgttgtcccc ctagcggtgg tgaaaccggg ctgtgattaa ttatccgaac tattaattga
      601 tcctttaaca acctcttccc gaaaggaaga ggttgttttt ttatcgtctt acatttccgg
      661 ctgtgtctgc atggcaaaca cccgtcatca gccgccgtct gtttttttcc ccaagcgatc
      721 atccatttct ccgatgtagg ctgcaagcgt atttccggct tcttcgattg cggtgcgcag
      781 atggttttcc tgctgctcga tgcgcgcaag aatttccgtt tttaattccg aacttttggc
      841 ttcctgtgcc cgcagctggc cctccaggta gtttcgcaat tcggtaaaac gcgataattc
      901 cgcctgatcc gcaagctccc gctgagcctt caaggccttg gcgtgctgat gggattccag
      961 aagcgctttg gcctggatat aaacgacaaa catcaggcat agcatgacga tcaaaaccag
     1021 catgcccagc attaccagcc ccagcggcat gtgcaccgag gtgaaaccga gcgataagtt
     1081 tgtcggcgaa ataaaagtgc tccagtttaa ggcggaaaac gctgcaatcg ccgccaggat
     1141 aatcaataac aacaaagaat gtagtttcat atccaatgcc tccactgatt tattttgaaa
     1201 tatgctgaaa atgtttaccg ggattctcta atcttccatt tctctataaa aaaaatgtct
     1261 atttcttcgc tggagattgg taatagcccg ttcatctttc atttagcata aaattacttt
     1321 tttcaaaatc aattctattc cgcatctttc ctctctgctt gacaaattct gttgaattcc
     1381 ttattcttct tcctcaaaac attaaggatt cggtagggat acatggataa aatatttaca
     1441 aaaaaaggcg ttttgttcgg ttttcttctg gtggctttca tcatcgtttt tgaaatcatt
     1501 ctggcctccc tttctcttcc cgcctggccg gccttcatgg tgatgatttt cttttttgtg
     1561 gtacacgaag acacaaaaca gggggctcct atcgtgatcg gcggactggc cggtatcttt
     1621 tgcgcggttc tgagtgaaat gttcattgac gtcctgatgc ccgttatggg acatgaagcc
     1681 gccaaacttc ttttcatcgg cctgttcgtc tatgcgattg tgcttttgaa ggacgccatt
     1741 ccttacgttt tcaacagcta cgccttcatg ttttttctcg tctccgcgat tgcccggccg
     1801 tcggcaacgg tttcgccctt tgtttggatg gccgtcgaac tcgttgtcgg cgttatattt
     1861 attctgggca ttctggggat caaccggatc gtctccgcga ttttgacgga agaaaaataa
     1921 aaagctcgtt gcatgatgat tgttacaaaa acaattaaac ttcaaaccca ggggttcaac
     1981 gatatggtca atatcactcc ccgggttctt tcccttctgg aagactcgcc ggttgccgac
     2041 gggctgattt gcgttttctg ccccggctcc accggttcgg tgacgaccat tgagtatgaa
     2101 tccggcgtac tgcatgattt gaaaaaggca ctcgaaagaa ttgccccgtc ggacatccct
     2161 taccggcacg atgaacgatg gggagacggc aacggctttt cgcatgtccg cgccgcactg
     2221 atgaaaccgt ctctgacgat tccgctggtc ggcggcaggc cgacgctggg aacctggcag
     2281 cagattgtct ttatcgactt tgacaaccga aaacgacacc gggaactggt tgtgcagatt
     2341 atcggggatg cataattcga ttcgacctgc atcgtgcccg atgcgaccgt ttgacagaca
     2401 ttcccgattt gctttcacga gtttctttgt gcagggagcc gcctcgaatt ccctgttttg
     2461 aattttttgt ttttgtggta ggctgcaaac ctcagaaatc aaagtttcac cgttttttga
     2521 ttaatttcct attcttttgc ttttaggggg tgcctatgaa aaaagttgtc atagccggtg
     2581 tcgtcatcat tgttcttgtt ttttcattta tcggtttcct tcatggaaac aatgcaaagc
     2641 cttcatcacc ttccggcgcg gcggtatcgt caggcatgcc gtccggagct gatacctaca
     2701 tgcttctgaa aaatgtcaaa ttcagatttg ccgaagatat tttcatcaac gttcaccaca
     2761 tgctgtcgat caccgaaaag atcaaacctt acccgtacgt ggatatgaat aatgatcaat
     2821 ccttcagcct taaaattatc agcggacatt gctcgctcga gccgccggtg atggaaactc
     2881 tcttcaataa acacgttttg aattataaag gcgcaccgct gaaagatctt aagctgtcga
     2941 cggtggaaat ggatattgac ggtcagaaag tgcccatgct gaaaacaagc ggcatgatga
     3001 aactggtcgt ctggcttccg tttgaaatga ttgccagagt cggcgtggac aagccgacca
     3061 accggatgac actggaagcc tataaaataa aatccgtggg gctgccattt gtcaaaaacc
     3121 tgatggatat gtccggcgtc aaactttcca cccttttatc catcgaaccc ggccggggtc
     3181 tctccattga aaaaaacacc atgtatgtcg acgcgctgaa catcaccccg ccgccgaaac
     3241 tggtcggcaa gcttcttgat gcgaaggcaa acgtcagtac aaactccgtc gatctgacac
     3301 ttggagagga aaaaccggca aacgtcaaat atacccttct ggtcccggac gcaaaaaact
     3361 ttatctatat cttccagggg tcactgatct tcggaaaact gcttgcgctt gatgcaaacc
     3421 tgcaactggt tgatgccaat ccggccgatt atctggattt ctatctgaaa aaatatttat
     3481 tacaattaag cagaagtgcc attcggatga agccggacgc ttcgctgata gtcaatattg
     3541 ccgattatca atccgattca tcgcccgctc cggcaaaacc agcggaaccg gccaagccca
     3601 catcgttatc caaggccgct ccggccaagg cggtttcgac aaaacccgtt gcgggaaaac
     3661 cggccaaaac gcccgctgct aaacccgctg cttcggcgcc cgcggtcaag cagcacgctt
     3721 cgatcaatct ccatctggaa ttcgatacgg ctcaatccgc tgttaaagaa caatatcact
     3781 ctcatatcaa aaaactggcc gatttaatga aaaaatcacc ggattctcgc gtcgtcatac
     3841 aggggcatac cgataatctc gacaaattca ataaccccga caataatata aaattgtctc
     3901 aggcccgggc ggacagtgtt cgtcagtatc tgatcgacaa attcggcatc aatgccgcac
     3961 gcatcaaggc tatcggctac ggaccgaacc aaccgattgc cgataatgcc actctggaag
     4021 gacagaagaa aaaccggagg gtcgaggccg ttattcaaac atcgccgcag gggatggtcg
     4081 catcaaccct ttagccccgt ttcataaaac agcgacaagg ctatccaccg gatacgccct
     4141 gtcgctgttt ttctttaccg gtaatccccg ttcacatgac cgtattttgg tttgttgctc
     4201 gagcaaaata aaccgaacgc ccctttgaca tgtggtaaaa atgatttaaa ttactcaaaa
     4261 agagtcgggt tgaaaatcga tgatcaggtt ggaaaagata atatccggcg ggcaaacggg
     4321 agcggacaga gcgggactcg attttgccat cgatcataaa atggactatg gcggatgggt
     4381 tcccaaaggc agaaaaacgg aagacggttc gcttccgctg aaataccatc ttcaggaaat
     4441 gccgacacgc gattacgcca tgcgaaccct tcagaatgtg ctcgacagcg acggcacggt
     4501 cattgtgtct cacggttttc tcaccggcgg ttcggcatta accagagaat tcgccctaaa
     4561 atacaaaaag tcctggcttc acattgatct gaacgcatta tctctgccgg atgccgccca
     4621 tatgctttta cactggctgg aggaaaagga aatccgcgtg ctcaatatcg ccggtcccaa
     4681 agccggcaaa gacccgaaaa tatataaggc ggttctgcgg ctgcttcagg aggcgctgac
     4741 ggattcccac gttttgggct ccggcaaaaa gtcatgcgtc aagcttcaat gcacgccgtc
     4801 ttcgaagccg ggatcgtccg gggagaaaca aaaataaaca cttgcgaaaa cggaacgaga
     4861 aactgttaac aaggaaaaac ggtttaacgt aaaggacgct atccgatgac caatcccaat
     4921 accctgttga tcagactttt aactcggctg cgcgatatgc gtctcgtctg ggatatcatg
     4981 ggatcccttt acaatggacg catctataat ctgatcgagg aactctacga cgatatcgcc
     5041 aaagatcttg acactccgga tcaggcgcag attctcgatg cgggcgcggg ccggggatac
     5101 ctgtctgtgg cgctggcatc ccggaatccg gatagccatg ttacaggagt cgattactcc
     5161 cggatgcagg ttcgccgggc tgaacgctac cgccgggaga aaaaaatacc gaactgctct
     5221 ttccgccaga acaatgtcct gtctctggat tttcagaacg ccgccttcga tgcggcggtg
     5281 agtgtcggat cgatcaaaca ctggccggat tcccgccggg gtctggcaga actttaccgc
     5341 gtctgcaaac ccggaagctg cctgatcatt tccgaaaccg accgcgacgt cccggacaac
     5401 gagctgcgca aatttgttca acgctttcgc ctgccttttg tgccggaaga tctgcttttc
     5461 tggggactgc gtcacgtggt tttcggacag agcttttctc aggatcaact ggccgacgcc
     5521 gtccgtctgg ccggattccg caatatcgaa agccacaagg taaaagcctg tccctacgtc
     5581 attgtaaaag catggaaata aaaccaataa cccttcctgg ctgattttca catccattcc
     5641 tccggccttc attatatgct tgccagatcg atcaatattt tgttaaacga caatccgaat
     5701 catttcggca aaggatgaaa aatattccat gacgttaaaa ggtattctca cggttgtccg
     5761 tattcccttt ctgattctgg ctctgatact gggcattctc ggcgctgccg tcgcctggta
     5821 tgaatccgtc cggtacggct cgccgttcaa cctctgctat gcggttcttg ccaccatcgg
     5881 gttgctgatt gcccacgcag cggtcaatat tttcaacgat tatttcgatg cccgcagcgg
     5941 cctggattat aaaaccaacc gcacgccttt cagcggcggc agcggcgccg ttccgaccgg
     6001 attactgacg gtacatgaaa gccttcaact ggggattatc tgctttgtcg tccttgtacc
     6061 gattgccgtc ttcttcatca tgaaaagcgg atggatgctt ctcccgctgc tgattgcagc
     6121 catgttgttg attgttttat acacgccgtt tattttaaaa atgggttatc ctgaatggtc
     6181 gcccggattg ggactgggca ttctgcctgt gatgggcgct tattttgtac acaccggaca
     6241 atatgctccg accgcgctgg ttgcctccat gccgtcgtat tttctggtgc acaatctgct
     6301 tctgctcaat gaatttcccg acgccgaagc ggatgtcacg gtgaaaagac ggacactgcc
     6361 catcgttgcc ggaaagaaaa acgccgcctt ctttttctcg ggtgtcactc tgctggtcta
     6421 tatctgggtc ctgggcgcag tcatttgggg gtctatgccc gtctttacac ttgtgtgttt
     6481 tctcacgatg ccgttggccc ttcaggttat tagaggttct tttcgttatg acgaaagaga
     6541 actcctgatg gacaccatga aaaaaaacgt gctgcttgtg ctgctgaccc aggcgctcat
     6601 tgcggcaggg tatattgccg ccggcatctt ctctcgttaa atcggatcaa ccctttaaag
     6661 cacctggtca tccggatgtt gccttcggcc cggtccccgg agccgacgat ttaccggccg
     6721 ccttggcgga ctgcggtttg gcctttttcg gtgacgcttt ggcgcgtttc gcgcctggtt
     6781 tggccgccgt ctttcccgcg gaaggtgttt tcactttttt ctgcccttcg gttttggtca
     6841 gttgccattc gatctttttg attttcgaat acgccgcctt tattttttta tcgtcgtcca
     6901 gacgtttccc tttatcaagc gccgcataag ccatacagcc cagttcctta atttgctcct
     6961 gtatcttcag gttaagattg aaaattttgt attgtctttt tccttcctcc gaaagttcat
     7021 tcattctcac ggacacgacg gttgcgcctt cccgcaagac ggcaatcccc tcttccatgc
     7081 cttttttcaa attggacttg atcttatcaa atatattttc catggtttcc tccttatctc
     7141 cttttaatgt gccgggtttc agaattgtcc atccagccac gctccggatg tttttacaat
     7201 gcggtcatga attgccgatt gacggatgcc cagggatttc ggaacatgaa aagaatgatc
     7261 gccgccttcg atcacatcca gttgccaagg tgcagacaga tttttcagaa ctttcctgaa
     7321 cagaatcata tcgcacaatg catctttcgt ccccgcaaaa aaaagcagcg gcacgtccag
     7381 gcggtacaga tgcgcatctc ttgttttggt caggtcgccg gcaggatgca gaggatatcc
     7441 cagaaaaata agcccgtcgg ccggcaattg tttttctgcg gccatttgag aggccactct
     7501 tccgcccatc gattttccgg acgcaatgac atgatcgacc tgaaaaggca tatcggtctg
     7561 gaaatgcaga aataccgcgc cccaggtcgc ttccagtttt ttttgaccgt ccggcgcctt
     7621 caggcctttt tctttatagg gaaaattgaa acgcagcgac ggataccccg cccgggcaag
     7681 cccgtcactg aaatcgacaa gcatttcatg ttccatatcg tttccggcac cgtgtgcaac
     7741 gatcacgccg gcgcggtagt gtttcttctt atccggcggc agagtcagaa ctgccgaaac
     7801 gttttcacct tgctggatgg tgatggtcag tttttttctt tccatagatt atttccgcgt
     7861 ctgttttccc tgatgatttt acggtttatc ccgatcatgt gcaacaagtt ttccgatggc
     7921 ctgatggata tccattttca taaaaccgga aattttccgt tcgacggcca gcgcctgatt
     7981 tttattgaga agaatccggc ggcggatttt ctcgtcggtg gcatatcgct taatcagatc
     8041 atccacccgc tcatcgaggc cgacaataac gttgtgcatc acccgcttgt cggcataata
     8101 aacgatttcc ctttcttcca gaccgccctg aagattcaga tccggtatcg tgacatgctg
     8161 ctccacaatc tcggcaacac gtcgaaatcc cagttttcgc aaaagcaccc ctccggagtc
     8221 agcgtgtttt tccccggttt gaagagacct tgttttggta atgtcatgca gcagcgctgc
     8281 ggcaacaatc aaatcccgat ttatcgacag tccctcctga agatggtccg taatggcaag
     8341 ggaaacccgc attacctgtc tggaatgctc cacaatgttc ggcaacatgg cataccggac
     8401 catcaggtct tcgcattccg caaggtccgg aatgctatct tttttatttt ccatcatcag
     8461 tttaagattt ctatttttta taaataatgt caatcaaaaa acctgttcgg ttttaaaaga
     8521 ggatgcaagg aagaaaatac accgggcgcc taaacccttc cacacttttt aaacaagtag
     8581 caccgaagaa tatttttgat tttgtcagtg taaacggcta tcgcaacttg catttttatc
     8641 acatacatga taggtaaaaa gaaaaatatc gaatggggtc caaaaagtca tcaggacttg
     8701 aggagatttt tcgatggttg ggtcgccatc tggcataagt cgggtgagcg atttttttta
     8761 tcccgttttg cgctgtctaa cgcacaggaa taactttttg aggaaataac gcaattgaga
     8821 aggacattgg tcggatttgc attatctttt ttttcagcgc ttttgctttt tacatttccg
     8881 gcaaacaccg gggcgttgtc caaagcggac gtgtcctttt catcggacac gttggaccgg
     8941 ggcggagtga ttcttgtcac ggtaaagacg aacgagcaaa aaaagccccg aataacatgg
     9001 ctgcaaaaaa aagtacccct ggtatacagc acgtcggacg aatcatggtc gggatttatc
     9061 agcgcggacc tgcatcagga aaccggatta tatgactgca tgatccggga gacctcttcc
     9121 ggctttcagg aaagatatcc gatccgtatc cgtttaaaag attacggcgt tcgccgattg
     9181 aaacttccca aaaccaaagt ggaactggat gctcattcct taaagagagt aaaaaacgag
     9241 gccgcggtca ttcattcgtt atggccggcc ggcgatacat cgccaaaatg gaaagggcat
     9301 ttcgtgatgc cggttgacgg agatatcgtg ggcacgttcg gcaagaaaag tattattaac
     9361 cagctgcaca gatcgccgca taccggtgtg gacctggccg gcaaaaaagg cgatccggtt
     9421 gtcgccatca atcacggcag ggttcttcta gcggccgacc attttttcag cggcaaatcg
     9481 gtttatgtga accatggcgg aggcatcatc agcatgtatt ttcatcttga caaaatattg
     9541 gtaaacaaag gagatgatat tcacaagggc gaagtcatcg gccttttggg tgcaacggga
     9601 cgcgttacgg gtcctcatct tcactgggga gtgagaatca acggcaaccg ggtcgatccc
     9661 ctgacgttga tcgcattgag cggcactctt gaagaataaa tggatgatac gttttttcgg
     9721 gaaagcacaa agcaatgaat cgataaagat ggccattgga caatcaatga tttttaaaaa
     9781 gaaaacgtcg cacggattta catttttaat atcgttcatc atcgttattt ttgcggttat
     9841 gtttttggcg acggacgttc atgctttgga cattagcaac atgtacagcc ctttgaataa
     9901 aaaacgtccg ttgcgttcga aaacccaata catcattttg catacgacag aaggcgctct
     9961 gaacggttcg cttcgcaagg ttcatcagtt cggggaaacg cactattttg ttacgcccac
    10021 cggaaaagta tatcgaatca ttgaccgcaa aaaaatcgcc acccatgcgg gcagaagcat
    10081 gtggaaccgg caaaccaccc ttgacaatgt ttccatcggc attgaagttg tcgggtttca
    10141 cgatcaagac atcacccggg cgcagtacac ggcgctccgt caactattgc gtcagttgaa
    10201 aaagatttac cggatcaagg ataaaaacat tctgacgcac tccatggtgg cttatggacg
    10261 gccgaacatt tttcatcccc gaaaacatcg gggccgaaaa aaatgcggaa tgattttttc
    10321 tcatcccgat gtcagaaaac gtctggggct tctgcccgga cctaaaagag atccggacgt
    10381 tgccgccggc cggctcgttg ttgccgatcc ggatctgcac cgttttctct acaaaccccc
    10441 gaaggtgttg gcggctttga aaggaacgaa ggattcatcc ggcagtcgat ccatggttat
    10501 tgcccagggg aacaatgcct ggcgcattgc ccgggataag tacgatgccc cctcgacaat
    10561 ttatacctat cccaacggaa agaaattgcg cggcaatgaa atccgcaaat gggacagcat
    10621 ccctgtgggt acccgcgttt catttgtgga tgaagaccgg gagcaggaat tcgaaaaatt
    10681 cctggaaatc ggcaaagatg ggaacacggt tcaggatctg gccggcaatc tttacgccga
    10741 tccgacaaca atctattttt tccccgacgg actgatccga accggcgcag aaatgaagaa
    10801 atcgtcgtct ctgcgcaagc tcctgaaaaa tccgcccaaa ggaacccgtt tgctggtggg
    10861 ttatatttac ggcgggtacg ttcaaaaagg gcgatccgcc acccggatcg ccggcagaaa
    10921 atggaattat ccatcgacat tctatcgaat gcctgacggg cgcattctca acggggacaa
    10981 cgtagagaaa aaatccattc cttccggaac cctgatcttc atcatgaaat aaaccgtttt
    11041 tacccggaag aatgctgcgg ctgaaacgga tcacatgccc ggtgaatcct tgataaggct
    11101 ggaggacgaa ccaagacgcg gtaaccgccg tcacggtcaa ctgtcaacgg aggcaaaaag
    11161 atgcattact cgcctatgat tttcaccagc acccgttttc tgcgtcttcc gtcgaactcg
    11221 ccgtaaaaaa tctgttccca cggtccgaag tccagttgac cgtttgtcac cgccacgaca
    11281 acctcccgtc ccatgatctg tcttttcaaa tgcgcatcgc cgttatcctc gccagtccgg
    11341 ttatgacggt aatgagcaac gggctcatgg ggcgccaggt tttccagcca gttttcgtag
    11401 tcctggtgca gaccggattc gtcatcgttg atgaacaccg acgccgtgat atgcatggcg
    11461 ttgactaaca cgataccttc atgaatgcca ctctcctcta aacaatcttc cacctgtgaa
    11521 gtgatattga taaattccct tcttttcttg gtattgaacc aaagttcttt tctgaacgac
    11581 ttcattcttt gcctccatta agcagctgaa agggttacct ttaacagccg caatacagca
    11641 tcatatatcg ttgggtcttt acgtaatctt ggccccgcaa cattgaggac tttgatttgg
    11701 ttttcagtca gccaggaaat cagtttttca gagaccggaa taaatatcgg ccctaacatt
    11761 tcatattgcc gcacatgcac atgcgcatgc tcatgccgac gattgatgga aagagattct
    11821 ctgttttgtc ccagggttat cgcgctaacg ccgccacgta caggcataca ttttttaagg
    11881 atgagcgata tgagcctgct gtgtagctcg agcacaccgt taaccacatg catcccgcca
    11941 cgggtaaaca aagccagcgg aacaaacaga agtcccagcg tcgtgttggg cagtgcccat
    12001 atatatcgca ggaatttcat tttaacgcct tgtccccaga attcccggta ctcacaaata
    12061 agcggttgtt atgcaactgt ttggtttttg ccctggcgtt tggcactata gagtgactgg
    12121 tcggctctat taattaaatc cgcgctgttt tcaccgggac ggagctgggc aagaccgatg
    12181 ctgacagtca ctgaaaattt ttgtccgtcg cttgtttcaa aagtatcatc actgattaag
    12241 cggcggattc tttccgcaac atccaaggct ttttcctttt cggctcccgg caggatgacg
    12301 gtgaactcct ctccgccgta tctgcaaccg taatccttat cgcgaatcga catcagaagg
    12361 atttcgccca gtcgtttcag cacctgatcc ccggcagggt gtccgtacgc gtcatttatc
    12421 tttttgaaat catccagatc cagtatcaac aatgacaggg gatcgcctaa attaccggaa
    12481 agggagattt cactttttag tttttcatta aaataccgtt tattgtacaa ttttgtaagg
    12541 ccgtcggtat tgcttaaaat caacagacgg tgttccctgt tttccagttc ccggttttcc
    12601 tgctgcaacg ttcttatccg gtccgccaag gccagggaga gtaaaatgga ttccgcggca
    12661 accgcgatcg gataagaata ggtaattaac tgtgtttggg gcaggatgcc caagctgcga
    12721 aacgcccagg tcagcatacc gacggataag accgaccatg cagttaaaaa atatccggcc
    12781 ggcttcatgc ctttgataaa acataccagg ccggcggtga tagccatgat tggaaaggtt
    12841 aagccgatgg cggcgcctaa aatctgcacc tgataataaa aacccgtaac agccattaat
    12901 atcaagagga tagcaaaaaa catatacgcc attaatattt tatcaataag cggcgcattc
    12961 ttggaagtca gcagaaaggt acggcagaac caggcggcga aaaaaccagt agaaccggtg
    13021 aatacgtaaa taagataaaa atgtgatccc gacagtatcc cggtaagcgc actgaaatga
    13081 ccgagcccta tggacaggta gaggaagttt gaaaatatgt ataatacata caccagatat
    13141 gtcttgtccc ggagggacaa atacatgcat gcattataaa gggtcatggc aataatcaat
    13201 ccataaataa tgccaaaaac ggtgaaatcc cacgtaagcc agttgcggaa ttcatttaat
    13261 cgtctaacat gcagcgaaaa attcagcggc ggtccccagg acgccatttt aagatacatg
    13321 ggacgacttt catcataatc tttggggata aaaaatatgg aactccggtt taagatgttt
    13381 atccggtttg attgatcaga tgtaacggca gaaagttttc tccagctttc tcccatgatt
    13441 tgtgccgttt tccgaatatc atggctatcc cggataaccg gaacccacag ctcgattgtc
    13501 ggcatccagg cacggtcaat ttccaggagc catgaattgt tatccaggca ctcatattcg
    13561 catgaatttt cggattgatc gaacttcagc agaaacctga accagtacgt ggatcgggtg
    13621 ataccgaaat ttaactgcgg gtcatttaac gggacaaaac gatcctgcgt gcccgggtga
    13681 agcagttgct ccaacgacag ttctccggca ggatcttcca aaaattcaag agcccggcag
    13741 atgtttactt ttggggaatc ttttagaaat gttggctcca gagcatgggc atgaacgcaa
    13801 tggaacacga tacctgtgac aagacacagc agccatagtc ttcttttata caccgtcatt
    13861 gaattgatta gaacttcctt tttactgtcg gcagtatagg aaacggtttc ttgagagtca
    13921 agaatattct gccacaagat atggggtgca actttattga ttacgaattg aaaggcagca
    13981 caattttcaa aatgagactg taaactaatc tgtgtaaatg gataattttt catattgccg
    14041 ccaaatcaaa acggccctcg tacataacag ccagctgaga aattgtcaaa gcccagttgg
    14101 ccaaaggcat cgttcatttt ttcatgacat cccgaatcgc cagatataag atttcggtca
    14161 gcgcctcatc attggaaaaa acagaccggt tttttgtcac cttgcgcagt tgcctgcgtt
    14221 catttttccg acgttcatag ctaatgactg tcgagattct gttcattaga tagttgtcaa
    14281 tttcttcaac accgccacct tgattgataa tcttttttat ctttacacgg tgatgctccc
    14341 ggattgactc tctcgtaaaa gcttcactca tattcgtgac tcatggctta ataaaggttc
    14401 tgcttcgtga ttaaaatttt tcttcataaa aattcttgcc aatttatcaa cacctccttt
    14461 gcgataaata aaaagtggac aatccgacga ctggcagctt aaaactgctc ccacatgacc
    14521 gttcatgcaa gcgacgcaat atgtgcgaat cgacttttgt ctttctttga cattctgctt
    14581 ccccttactc gtccgatatg aataaagagg gcaagaattc atttgcagcc ggcaacatct
    14641 tttgggctcc atgcagaaca gttaaggtac ttttcgtgaa tggcctttcg tcggttcaat
    14701 gacactattt gaacaccatt tttgcctatt attttatttt tcattctcat ctccaaaaac
    14761 agaaaagcct ttggccatta atagccaaag gcttctttaa tacgaaatga atgcagagcc
    14821 gtgacttcgc ccttaaatgt aaaaggaata acttttgcta aaagtactct ttcgttgtat
    14881 taatcaaggc cagtccttcg cgaatcccgc tctcgcgcag acaatcctcc acctgacctg
    14941 ttatgttgat aaactcgcgt ctgtgcgcgg tattaaacca gagttctttc ctgtatgatt
    15001 tcattaatct cctcccggaa tgatttaaaa tccgtgtaaa gaatcgcctt ccatcctttt
    15061 tgtcgggcac gttgaatatt gcccggatcg tcatcaataa ataaaatgtt ctccggtgtt
    15121 tctctcagta agccggcaat atcatcaaaa agcgacggat ctcttttccc tttgccgaga
    15181 tgataactgt taaagacata atcgaaatgc ctgaagaaat caaaacgtgc attcagtccg
    15241 tccagccagt ccgtctgatc gctcagaatg ccgacaataa aattttccga cttcagtttc
    15301 cggaccgctt ccatcatggc atcattgagc gcaaacctcg aaatcagttc acccgataac
    15361 gaagcgacat ccccccggat gccggttttc gttttgagag atttccagaa atcgctttgc
    15421 gagccttttc ccagaatata accggtttca taaatcgtat cggcggctgt ttgaaggaaa
    15481 ctgtcggggt gaactccgtt cgcttttgcg atgattctaa gccctttttt ccaaccctct
    15541 tccgagagca cgccgccaaa atcaaacaaa acgacatgaa cacgtttatt tttacaattt
    15601 ttattttcca acaatttata actccctgaa ctcggccatc cttcttatca ttacatccat
    15661 tttgaccgtt ttccattttt catttcaaac atcctttgat caccgaaatg acacaaagaa
    15721 ccatcaacca ttaactatca actatcaact aaataatttt gtcggacatc cctccgtttg
    15781 tcatgcccga gtgggcttgt cgggcatcca gtcttctttt ttcctgtata ccggccaggc
    15841 ccgcgccggt atgactcgga ggtgttgtgt tacttcgaac gaatttaaga agtcttaaag
    15901 atttctcgcc actgcgtttc tcgaaattac acggaggggt gctttgcacc cgacgtgacg
    15961 ctccgattta acgtgaaaaa aaatatccaa taacgcaaag catgataaac acggcgatgg
    16021 ctgaataaag tatgatgcga agttttgtta tgtctttcat gattcatttt ccttgacggc
    16081 ttgtattcta ttgtgaattt cgggcaggaa gaccggaacc tttgctgcct gatccggcta
    16141 tatcggcaaa aaaacgtcag gattctttgc tcccccatcg caaggaacgt cttcggcgaa
    16201 ccaattcatc gtataaatta tccagcggca catttttccc gcctccctca agcatcgcta
    16261 ccgcagccgc caccgcctcc agggtggaca tataattcgg acagtgctga tcgcgcaatc
    16321 tctttctttg ggcgacaggc gcctccaggg ccaggcgagg catccgccaa aaagcgggca
    16381 gactgctgta caaccggcgg gcctgttgcc atgttgcatc aatcaccacc acccgagccg
    16441 gggattgcct gtcggatgtc cgcggaagcg tgccggctcc gtccggccac aacagccagg
    16501 ttccgggagc attcagcaga tcgtcatcca cgcacgccag accgatgcgc tgacctcctc
    16561 cgcaggaaat gagttttgaa ttcgtaagcg ccagcgcagc cagacggcct gtgttgctgg
    16621 gcttttccgc ttcgtaaata tgccggagga tcagaaattc cacgctggtt tccaccgcgg
    16681 ggatgatggg acacaggcag atttcccgac gctgataaca gcgtgagcag tgtgtgttat
    16741 gattcggcaa cctgttcgaa cgcattcaaa atactttcac attattttgt tgcccagtat
    16801 acgtaaagcc tttttatctg tcaacggccc cgccgtctaa aattctgagc tgccatccct
    16861 gcacgcactg cgatttattt gcctttgcgt tgctaatcct tttctctaaa cggctgcgaa
    16921 actgtcctca acggcggcac cggtcaggat aaccggccgt aagtgcacgg tccccgcctg
    16981 aatgagcgcc tggcgcacat tccccttccg ctgccattcc atctaaataa aatccaccag
    17041 cagaaggaaa ttacagacaa caacaaatgg tgcagcctcg gcagaggctg caccatttca
    17101 aaaaagccct tgttttatgg tgctcccatc attttccgtc tttattgacg gaacctcgac
    17161 agccagtgtt ccatgagttt ccctgccggt tcacgtccgc ccagaccgaa ggataacgcc
    17221 acggtcaccg caatggcgcc caacgtcaat ccgaacgcga ggttgacaat atcatcggca
    17281 atgcccatag cacgcagacc aagcgccaaa accagaccca aaatggcgaa acgtccgatt
    17341 cgcgccagcc cctttgtgtt gggtccgctg gctttgtcca tggcttcata ggcaaggttg
    17401 gccagccaga atcctatcac aagaataagc gatcccaaaa gcacatcgcc gccgaagcgg
    17461 atgaatgtat ccaccgtatc gctgacgtgc gtgaatccga gctgatttgc ggcttcaacc
    17521 gtggcgaaaa gcatggcaaa aaacaagacc acgtatccca caaaaaccga tgccggtgtt
    17581 ttttgaaacg catgttccaa tgcgatgtgt ttcggaatgt tgtcaaagcc cattgcattg
    17641 aggagactag ccaaaagccg ggaagcaaaa gaagcaacca tccaggtgat caccagaata
    17701 agaccggccg caacgatatg aggcacagcc atcaaaatca tgtccaacat atccattgcc
    17761 ggtttggaaa tcgcttctat ttttaatgcg tccagagcgg cgatcagcgc cggtaaaaac
    17821 accgccacga aaaccagcag gcctatcgcg ttggaaagct taacggattc cgccaacccg
    17881 accttctgac tgagtttgtc acttccggcg gtacgcaaaa gattggttgt cagattacgc
    17941 agaaccgtgg ctaaaatcca acccacgaag ccaatggcaa cggcggctgc cgcgttgggc
    18001 agaaaatcaa gcactttggt aatcatatcc cgaaacggat ccagaagacc gtccatttgc
    18061 agtgcgccca taatggccgg tacgaaaagc aggatgatga gccagaacaa ggcttgactc
    18121 agtccgtcac tgatgggcga catgcccgca tgttcggaaa gtttttcatc cagtgtggtt
    18181 ttggaaagcg ccttggtgac gaacctgcgt acgatgcttg ccagtaacca ggcaatcaga
    18241 gcaagcatca acccggccag cagatgcgga atgtatgcca gaaattgtgt caagagcgcg
    18301 gcaaaggggg cggaaaccat cgccagatca agcgagttta acacggccgt tatgataaaa
    18361 agcataacga tccaaaaaat agctattgcc aagccatttt caaggttgat gctctgacct
    18421 gtaagttgag cgaaacgcgt gttcaaatta agcttgccca gtccccggcg gacgcccgcc
    18481 cgtgccaata cggcaagcaa ccaccccaga accagaacag ccaatgctcc caaaagggcc
    18541 ggaatgtgat ttcccatgcc actctgcatg gcattccaca tcgttgtcaa atttgaattc
    18601 atgtttttcc ctccttttta taatatttgt ttaataatta tatttcgggt tgaaaaaacc
    18661 aattaacgac tatgtattga ttatctgaag cgttggttgt ttcgttgcaa atatgcgttt
    18721 tactatttga tgatattgaa gcttatttgt gaatgaatat attataaatg aaaatacatt
    18781 tttttaccag cgaaagaata ccgcctgcaa gaagcgcata aattgtcctg tttgcattac
    18841 ataaactgtc cgatgggtca gttgccttat aaaaaaagcc ggcccaatca ttcttcaatt
    18901 gaaccagcta aaaaaaatgt caagagtaaa aattttaccg cagatccggc tacggcaacc
    18961 ggtaatacca tttaggcccg cgtttaatga cagccgctga
//
LOCUS       DAOOLB010000036        18714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_3029_length_18714_cov_50.379495, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000036 DAOOLB010000000
VERSION     DAOOLB010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 18714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18714
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3029_length_18714_cov_50.379495"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..594
                     /locus_tag="PLF77_06115"
     CDS             88..594
                     /locus_tag="PLF77_06115"
                     /inference="COORDINATES: protein motif:HMM:NF001664.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPC08463.1"
                     /translation="MTQLKAVQANIVTLKVDAIVNAANQSLLGGGGVDGAIHRAAGPE
                     LLAECRTLGGCKTGEAKLTKGYNLPANFIIHTVGPVWHGGKSGESQLLASCYRSSLIV
                     AAENGIKTVAFPSISTGVYGYPVEPAAQVAVKTVRDTVQKLSTIEEVIFCCFSGKDLE
                     IYEACLKN"
     gene            complement(988..2070)
                     /gene="asd"
                     /locus_tag="PLF77_06120"
     CDS             complement(988..2070)
                     /gene="asd"
                     /locus_tag="PLF77_06120"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF006416.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPC08464.1"
                     /translation="MLKIAIVGATGIVGQQAIVSLLGHPWFKITKLVASERSAGKKYI
                     DALRDANGSIRWWCPEELPGHIANMVVEEAARFDPKSVDIIFSTMDAGPARDLEPKYA
                     KTTPVISTASAFRYEPDTPILVGGVNMDHAALLAVQQKNRGWKGFISPKSNCTIVGLV
                     VSLKPILDNFGVKQVVATSLQAMSGAGRTPGLSAMDMTENVVPYIPGEEPKVETEPLK
                     ILGKLTGKIIVNAAFGITATCTRVPVLDGHMVCAFVQTKKKCSPEDVKKAMMNYGKDF
                     AELHLPSSPAHLIDVTDDPFRPQPRMDRDKGGGMTVTVGRIRKDPVIENGIKYVCLAH
                     NTKIGAAKGALQTAEYLVKNYLKWVK"
     gene            complement(2087..3037)
                     /locus_tag="PLF77_06125"
     CDS             complement(2087..3037)
                     /locus_tag="PLF77_06125"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPC08465.1"
                     /translation="MIIFRSTENITGDFRGSFVSIGNFDGVHLSHQFICRKLASEAKQ
                     AGTKSLVITFDPHPKMILHPNIRPFFLITTLDEKLERLAACGIDGVLVIPFTMEYSKT
                     TAEEFVHNFLGRKLAIGKIIIGHDYTFGQGKKGNSDYLIAAGRDMGFAVEVVDAVKSG
                     NDIISSTLIRKSIIEGNLKKAAVLLGRYYNVAGKVVTGFGRGVTLGFPTANIEPEKEL
                     LPPPGIYAAFVDAEEKRYQAVLNIGAKPTFEDYTFSFEVHLLDFSGDLRGKRINTDFV
                     EKLRDIVKFDSPETLKKQIAADVEKAKTILLENISNRQGT"
     gene            3154..3240
                     /locus_tag="PLF77_06130"
     tRNA            3154..3240
                     /locus_tag="PLF77_06130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3188..3190,aa:Leu,seq:cag)
     gene            3410..4075
                     /locus_tag="PLF77_06135"
     CDS             3410..4075
                     /locus_tag="PLF77_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HPC08466.1"
                     /translation="MLSVLAAYLGGVIISFTPCTYPLIPVTVGLIGIQGPSSKIRGFF
                     LSLFYVAGMAVTYAVLGAAAALSGKIFGQMQTTPWTYFIMANLCLVMGLSMLDVFNLS
                     IPVPQKLMQWTGANGKKGFLNSFLIGAVSGVVIGPCTAPALGVLLGYVAVKTNVFLGM
                     SLLFIFAFGMGTLLIIAGTFAGMIAALPRSGAWMEKIKFISGLILIGAAEYFLYIAGR
                     LSI"
     gene            4085..4594
                     /locus_tag="PLF77_06140"
     CDS             4085..4594
                     /locus_tag="PLF77_06140"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPC08467.1"
                     /translation="MMNKKILLAIALVFVFALVAFSFEQFKPSVFTRRDKVSAPDFSL
                     KDIHGKTFRLSAQRGNPVIMFFGTTWCPACREEMPLYRNLYNKYAGRGLKFLYIDINE
                     SAERVARFAGQNAFPYHVLVDGDGSVAGDYNIIGVPTLILLDREGNIVDVGHRISDLP
                     VDRLFSVKK"
     gene            4618..4830
                     /locus_tag="PLF77_06145"
     CDS             4618..4830
                     /locus_tag="PLF77_06145"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPC08468.1"
                     /translation="MNERNWLQNLTRSNTDKWIGGVCGGLGESSPVPAWAWRLLFVTL
                     VLCFGTGVLLYILLWIFVPDKSTIQE"
     gene            4833..5498
                     /locus_tag="PLF77_06150"
     CDS             4833..5498
                     /locus_tag="PLF77_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC08469.1"
                     /translation="MRLKNDIYLYEWTNPYENNCNSFYVGGGVNALIDPGLTGYVPDL
                     LDRMSADGIQKEDIRYVINTHSHPDHFQGSELFDQEKVKVGLHTREIEFLKGDGGELY
                     SLFGINAPRMHVNLPLEEGDLALGDQIFKVILSPGHSPGSIGLYWPDQKTLFCGDVIF
                     DQSVGRTDFPGGNGDLLKKSILAFAKLDLEWLLPGHMGILHGKGSIQNNFNLIIKGIF
                     PYI"
     gene            5527..7212
                     /gene="ettA"
                     /locus_tag="PLF77_06155"
     CDS             5527..7212
                     /gene="ettA"
                     /locus_tag="PLF77_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPC08470.1"
                     /translation="MANEGNKVIYSMIGVSKIYDKKPVLKDIYLSYFYGAKIGVIGLN
                     GSGKSSLLKILAGVDQEFLGKTILSPGYTVGYLEQEPHLDNDKTVRQIVEQGVQSTMD
                     LIQEYNDINEKFAEPMSDDEMAKLCERQGAVQEKLDALDAWDIDSRLEMAMDALRCPP
                     GDTSVKVLSGGEKRRVALCRLLLQKPDILLLDEPTNHLDAESVAWLEHHLQKYEGTII
                     AVTHDRYFLDNVAGWILELDRGQGIPWQGNYSSWLEQKQNRLKNEEKAESDRIKTLER
                     ELEWIRMSPKGRHAKSKARISAYEELLGKTMEKESGTREIFIAPGPRLGDLVIEAKDV
                     NKGYGDKLLVEGMTFSLPPGGIIGVIGPNGAGKTALFRMITGREKPDAGTIRIGDTVK
                     LAYVDQSRDSLDPNKNIWEMISDGQDIIAIGNRQINSRAYVSRFNFSGTDQQKKVGTL
                     SGGERNRVHLARMLKEGGNVLLLDEPTNDLDVNTLRALEDALENFAGCVVVISHDRWF
                     LDRICTHILAFEGDSKVLFFDGNYSEYEEDYRKRYGNAASQPHRIKYRQLTRR"
     gene            7537..8856
                     /locus_tag="PLF77_06160"
     CDS             7537..8856
                     /locus_tag="PLF77_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPC08471.1"
                     /translation="MNKKRLVLLVILFITIGLFSLQQPSENSFRGDAVVSEPTAPEKG
                     TRPLKSISDKFEKGETLSSIFKKYGMHTDDLFELKSAVASIQSLRDIKPGKPYSFTLN
                     EDNYISSFTCGIDDDTILKIERVDGCFQAQKYNIPYETRILTIGGTIQNTLVSAFGNE
                     REDYLLALQAADILAWDIDFNTDVRKGDTFRIIVEGLYSEGKFKKYGRILSIEFVNGS
                     QTYNAYLFEQDGKTDYYNADGQSLRKAFLKAPLSFRRISSHFSRSRLHPILKVRRPHH
                     GIDYVAATGTPVSTVADGRITFAGYKSGYGKIVIVNHANGYQTYYGHLSRIKSNIRPG
                     KKVQQGDLIGNVGATGLATGPHLHFEMRQGGRAINPLAVKKVAGSPVPAAQMAEFKNT
                     TQTMNNIYTVALFFDTNNTQRKNHSLQPALLYASLASSSPQGKFRKN"
     gene            complement(8907..9242)
                     /locus_tag="PLF77_06165"
     CDS             complement(8907..9242)
                     /locus_tag="PLF77_06165"
                     /inference="COORDINATES: protein motif:HMM:NF025015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HPC08472.1"
                     /translation="MPFKTLITVLICNFIFVMIAVPLALRKVPKNVIYGFRIKATLEN
                     DFVWYEANAYFGKVFIIGNMVCALLILILYFSDVVPAQNFVNIGMAILIIPALVAALL
                     TFRTIKTKH"
     gene            9354..>9941
                     /locus_tag="PLF77_06170"
     CDS             9354..>9941
                     /locus_tag="PLF77_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HPC08473.1"
                     /translation="MNNTKIPTVKPFVHHLRPRGLSRRIPLIETLRNYRREWLMHDIL
                     AGLVLTAILVPVGMAYASVSGLPAIYGLYATIIPLIAYAIFGPSRILILGPDSALAGL
                     IAAAILPLAAGNTDKAIALAGLLAILAGAFCILAGLVKFGFITDLLSKPVRIGYLNGI
                     ALTVLIGQLPKVLGFPVGGNNILQEVNSLIQGIAGG"
     assembly_gap    9942..10041
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10042..11275
                     /locus_tag="PLF77_06175"
     CDS             <10042..11275
                     /locus_tag="PLF77_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HPC08474.1"
                     /translation="GNNILQEVNSLIQGIAGGQINWMSCLIGCSCLMIILGCRQWFSR
                     IPGVFIAVAGATIISSWFHLSASYGIAVVGPLPQGLPQFQIPSVSFAEFKALFAAAFA
                     IALVSFADMSTLSRTFALRAGQEVDSNQEIIALGAANMAAGLFQGFPVTSSASRTPVA
                     ESAGAKTQVTGVVGAVCIALLLIFAPNLLKNLPQAVLGAVVICACISIVEVRALARLY
                     KLRREEFVFSIACFLGVVSLGIIKGIFIAIGLALFSFIWRAWHPYDAVLGHVEGLKSY
                     HDISRHPDARLIPGLVLFRWDAPLFFANAEAFRENVLHAIAEAPTPTKWVVVTAEPIT
                     DVDTTAADMLAELDNVLHESGMALFFAEMKDPVKDMLKRYGLFTRFGEENFFPTIEQA
                     VERYLTLHKIDWPESHKR"
     gene            complement(11539..11859)
                     /locus_tag="PLF77_06180"
     CDS             complement(11539..11859)
                     /locus_tag="PLF77_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08475.1"
                     /translation="MSSELLRYEINRKIRQVLVSHNADMTKISYSFVHRTVYMSGNLV
                     RESQGEFSLPVIEGMIRELMKLPRVQKILFDLENWIISNEPGALNIVKKKGLGQHPAI
                     KDSV"
     gene            complement(11892..12191)
                     /gene="yhbY"
                     /locus_tag="PLF77_06185"
     CDS             complement(11892..12191)
                     /gene="yhbY"
                     /locus_tag="PLF77_06185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HPC08476.1"
                     /translation="MEKLKGSQKKYLRAQAHHLKPVVMIGAKGSTTQLISSVNAALND
                     HELIKIKFGEFKEAKKEISQEIAGKTKSELVGLIGNIAIFYRQNPNPERRKIKLS"
     gene            12445..13407
                     /gene="trxB"
                     /locus_tag="PLF77_06190"
     CDS             12445..13407
                     /gene="trxB"
                     /locus_tag="PLF77_06190"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPC08477.1"
                     /translation="MSKANLPQYDCDTIIIGGGPGGYTAGIYAARSGLKSLLVEGAST
                     VSQITITDLIENYPGIPDGINGFDLMQLFKKQAQNFGLEIIANDVHSIKKAGDNPAIW
                     EIAVGDKSYRTLSVIAATGAQWNKLGVPGEEEFAGKGVSYCATCDGPFYRNRDVVVVG
                     GGDTAIQEALFLTHFAKKVSVIHRRDRLRAAAILQKRAFAEKKIEFIWKAKLAEIAGQ
                     EFVTGVRVADVESGKINHIEAEGVFIFVGLTPNTALFRDVLNLDKGGYIITDDNMRTS
                     APGIFAAGDCRAKQFRQVVTAAGDGANAIYSAELYVDELKGQTY"
     gene            13537..14370
                     /gene="lgt"
                     /locus_tag="PLF77_06195"
     CDS             13537..14370
                     /gene="lgt"
                     /locus_tag="PLF77_06195"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPC08478.1"
                     /translation="MEAFINTWQQLPSHINPALFTIGSFQLRYYSLMYLVGFAIVYLL
                     TTYRISNEHYEYKKEMLQDYLIWGMFAMIVGARLGEVLIYNFSYFSQHPLEIFLPFSF
                     SNGIHFTGISGMSFHGGVVGVMIYSIYFLRKRQINFWRFADLIVPSIPLGYTFGRLGN
                     FLNGELWGRVTTMPWGMYFPFDPTQHLRHPSQLYEAFFEGIFLFVILWLLRKKKFADG
                     FLLGLYIFGYGFVRFMIEFFREPDFMVGSVSIGQVLCVLMMAAGLIIVLWRRQIASPA
                     R"
     gene            14384..15715
                     /locus_tag="PLF77_06200"
     CDS             14384..15715
                     /locus_tag="PLF77_06200"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPC08479.1"
                     /translation="MGKHLIIVGGGAGGASAAAEAKRNDPELNVAILQSGKFIAYASC
                     PTPYYIGDIIKDENRLVARTPEEFRKRGVEVQLDTEVVNINCNANVVETRDNLMLPYD
                     YLVIGTGTNPFVPDIPGRELPGVYVLNNLENAIHIKAWLRERKAKRVVIIGGGFIGLE
                     MSEALHEVGISTTIIHKHSLPANRWSKELSSVMLEELQSHSVEFIGNAQAKAIEKSSG
                     DSLKVITDAGEWQGDMVLLAVGVRPNAALAKSAGLTIGKTGAIAVNFAQQTSVENIYA
                     VGDCCESWNRVSRRWVNIPLGDIANKQGRVAGRNIGAKPLTFDGIVGAQSFRLFDLEC
                     AATGLSEKEALAAGFVPISNITWGSAMAPAMGMKKIGLKLTADKSSGKLLGAQAVGVA
                     GAVGRINALSVALWTGLNIDEVGYLDLAYAPPFSAAWDIIHNAAQALRRMI"
     gene            15734..16672
                     /locus_tag="PLF77_06205"
     CDS             15734..16672
                     /locus_tag="PLF77_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HPC08480.1"
                     /translation="MTLWWTIFGVFILAMLALDLGVFNRKAHVIKMKEALLWVSFWVS
                     LAVAFGVGVYYFYGHSKALEFFTAYIIEYSLSIDNLFVFLLIFRFFNVPQAYEHKALF
                     WGILLALITRAAFIFAGVALINKFHWIMYLFGAFLIYTGIKLAMNKQTEVHPDKNIAI
                     KMLRYVVPVTKEFHEARFFIIKNGKRFATPMLAVLLALETTDILFAVDSIPAVLAISR
                     DPFIVYTSNVFAILGLRSLFFAISGLMKLFHLLHYGLSAILSFVGVKMLIADFYHIPV
                     GISLTVISSILVISIVASIIWPDTKYEDIPNITMKD"
     gene            16677..17456
                     /locus_tag="PLF77_06210"
     CDS             16677..17456
                     /locus_tag="PLF77_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPC08481.1"
                     /translation="MQNGFLTPSQIQLKKWARLDDAKARHEEGVFLAEGVKVVEELLK
                     SDWQIKALLVMPEKIKFWKKLDLSREEEIPVYQLKRTQWEKIGQDREPEGIMALVEMK
                     QAPDFSSWLAQTSGNVLILHEINNPLNLGALARSAQWFGFNSIILSANSVDFTNPKAI
                     RSSMGSLFHLTMIPELDLMETLPEIRKHFFIIGSDVHEGTAPHPVQKNAALTLGNESH
                     GLPATILKMTDEEWSIPGSGKADSLSLPQAAAIMMYECTKK"
     gene            complement(17579..18421)
                     /locus_tag="PLF77_06215"
     CDS             complement(17579..18421)
                     /locus_tag="PLF77_06215"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HPC08482.1"
                     /translation="MKSKIKILVFLCLAISVMFSLVNCRQQNGACDCPPSKFENGIYY
                     SQTYEDYILAHVFQDVANGFYIDVGANDPNIFNVTRFFYERGWHGINIEPNAELYLQL
                     RSFRQRDINYNVGIATTEGRMIFYQQAYKSDALMPLSTFDKEIVDIQKKNGILFKEIV
                     VPVTTLNNLLGKISVREITFITIDVEGFEKQVLESIDLARYKPVVLCIEASRPGTGDL
                     SYMLWEQIVLNNNYVFAMSDGVNRYYLHKSHIELLPRFIDIDRCVKLSKYKRQVKMDG
                     WSSW"
     gene            complement(18517..>18714)
                     /locus_tag="PLF77_06220"
     CDS             complement(18517..>18714)
                     /locus_tag="PLF77_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08483.1"
                     /translation="HIMIAAACGKLSESALDDEQICHYLHWIAYYLFDLTKSRKNKQQ
                     RNCFRNYTDPVSYYSWNREKA"
BASE COUNT         4543 a         4578 c         4622 g         4871 t
ORIGIN      
        1 caggccatga agtaaaactt ctaaatcgag caccactgcc cgctgattcg ggcagcggtg
       61 ctatctccat gccttggagg cggttcgatg actcaattga aagcggttca ggcaaacatc
      121 gtaaccctta aggtcgatgc catcgtcaat gcggccaatc aatcgcttct gggcggcgga
      181 ggtgtggacg gagcaattca ccgggccgcg ggtccggagc ttctggcgga atgccggaca
      241 ctcggcggtt gtaaaaccgg cgaggccaaa cttaccaaag gatacaatct tccagcaaat
      301 tttatcatcc ataccgtagg accggtctgg cacggcggta aaagcggtga atcccaactg
      361 ctggcgtcct gttaccgcag ttctctgatt gtggccgccg aaaacggcat caaaaccgtc
      421 gccttcccga gtatcagcac cggtgtttac ggatatccgg ttgagcctgc ggcgcaggtg
      481 gcggtaaaaa ccgtgcgcga tacggttcag aaattatcaa cgatagaaga agtgattttc
      541 tgctgttttt ccgggaaaga tcttgagatt tacgaagcgt gtttgaaaaa ctgatttttc
      601 cgaggctgca tgcaacgtgc tttgacggat gcatttttaa acaacggacc gcggcatttt
      661 taaatcatgc cgcagtccaa tgaacaaccc cgccccaagg ggcggggcat tagacccgca
      721 tttatcccgc tcggcctata gtgaccttta ggttattcgc ctcggggata tcaaggatat
      781 ctgcgatatc ctccgcctac ggcgggataa ttcaacttac aagtttcaga agcgtagcaa
      841 acttaaactt gagtttcatt aattcgacca tcaaatttca gtgcacttcc accgctttgc
      901 gggataattc gacttatgct tcaatcttcg cttcgctcga ttttagcaag tctcactacg
      961 tttctgaaat ttgggtctca ttaaatttta tttgacccat ttcagataat tcttgaccag
     1021 gtattccgct gtctgcaggg cgcctttggc cgcgccgatt ttggtattgt gcgccagaca
     1081 gacgtatttg atgccgtttt caataaccgg atccttgcgg atgcgtccga cggttacggt
     1141 catgccgccg cccttgtctc tatccatccg cggttgcgga cggaacggat catcggtcac
     1201 atcgatcaga tgcgccggtg aactgggcag atgcagttcg gcaaagtcct tgccgtaatt
     1261 catcatggcc ttcttgacgt cttccggcga gcattttttc ttggtttgaa caaacgcgca
     1321 aaccatatgg ccgtccagta ccggtacgcg ggtacaggtt gcggtaatgc cgaacgcggc
     1381 gttgacgatg atttttccgg tcagcttgcc cagaattttt agcggttcgg tttccacctt
     1441 gggttcctcg ccgggaatat aaggcacgac gttttcggtc atatccatcg ccgacaggcc
     1501 gggtgttcgt cccgcgccgg acatggcctg cagcgaagtc gccaccactt gttttacgcc
     1561 gaagttatcc agaatcggct tcaacgaaac aaccagtccg acaatggtgc aattggattt
     1621 gggtgaaatg aaacctttcc agccgcggtt tttctgctga acggccagca gggccgcgtg
     1681 atccatattc acaccgccca ccagaatggg ggtatccggt tcataccgga atgccgacgc
     1741 ggtgctgatc accggagtgg ttttggcgta cttgggttcc agatccctgg ccggtcccgc
     1801 gtccatggtt gagaaaataa tatccacgga tttcggatcg aaacgggctg cttcttccac
     1861 caccatattg gcaatatgcc ccggcaattc ctccgggcac caccagcgga tcgaaccgtt
     1921 ggcgtcccgt aacgcatcaa tatatttctt gccggccgat cgctccgaag ccaccagttt
     1981 ggtgatttta aaccagggat gtccgagcag actgacaatc gcctgctggc cgacgattcc
     2041 ggttgcgccg acaatagcaa tttttaacat aattcaaaaa cctcctctaa gttccttgac
     2101 ggttgcttat attttcaagc aaaatcgttt tcgctttttc cacatccgcg gcaatctgtt
     2161 tcttcaacgt ttcgggagag tcaaatttga caatgtccct cagcttctcc acaaaatcgg
     2221 tattgatcct ttttcccctc agatcacccg aaaaatcaag caggtgtact tcaaaggaaa
     2281 aggtataatc ttcaaacgtc ggtttcgctc cgatatttaa aacggcttgg taccgttttt
     2341 cttccgcatc aacaaacgcc gcataaatgc ccggcggcgg cagcaattct ttttccggtt
     2401 cgatgttggc cgtcggaaat ccgagcgtga cgccccgtcc gaaaccggtg accacctttc
     2461 ctgcgacatt gtagtatcgt cccagcaaga cggcggcttt tttcagattg ccttcgataa
     2521 tggatttcct gatcagcgtg ctgctgatga tatcgtttcc cgatttaacg gcgtcaacga
     2581 cttcaaccgc aaaccccatg tcccgtcccg cagcaatcag ataatcactg ttgccttttt
     2641 tcccctgacc gaaggtgtaa tcatgtccga taataatttt tccgatggcc agtttccgcc
     2701 ccagaaaatt atgaacgaat tcttccgccg tcgttttgga atactccatg gtaaaaggaa
     2761 tgaccagaac gccgtcgatt ccgcaagccg caagccgctc cagtttttcg tcaagcgtcg
     2821 tgatcagaaa aaacggcctg atattcggat gaagaatcat cttgggatgc ggatcaaacg
     2881 taataaccag tgatttggtc ccggcctgtt tcgcttcgga ggcgagtttc cggcagataa
     2941 actgatggct taaatgcacg ccgtcgaaat tgcctatgga gacaaatgat ccgcgaaaat
     3001 ctccggttat gttttccgta ctcctgaata taatcattgt ttcatactat aaacgtcaac
     3061 cagtagcttg ataacataac atgttggaca ttaaattcaa gctcatttta tcttgacaag
     3121 aacgtgccga tcggtatagt gcactttttg tgtgccgaag tggcggaatt ggtagacgcg
     3181 ctaggttcag ggtctagtag gcgtgcgcct gtgctggttc aaatccagtc ttcggcacca
     3241 ttttttatca ttattgattt gcataatcat aacttgtcac agccgaaaac gatctttttc
     3301 tgttgatttt ttaaactttt ttatccggta acgaacaacc agacacaggc agtatttgat
     3361 gattgaaccc cttgttaata atctgtcatt ttatcttgaa agctcgctga tgttatctgt
     3421 ccttgccgct tatctggggg gcgtgattat cagttttacg ccctgcacgt atccgctgat
     3481 tccggtaacc gtggggttga tcggcatcca gggaccgtca tccaaaatcc gcggtttttt
     3541 cctctcgttg ttttatgtgg ccggaatggc ggttacctat gcggttctcg gtgctgcggc
     3601 ggcgctgtcg ggaaagatat tcggtcagat gcagacgacg ccctggacgt attttatcat
     3661 ggccaattta tgtctggtca tgggattgtc catgctggac gtgttcaacc tctccatccc
     3721 tgttccgcaa aagctgatgc aatggaccgg tgcaaacggc aaaaaaggat ttctgaacag
     3781 ttttttaatc ggcgccgttt ccggcgtggt catcggaccc tgcaccgccc cggccctcgg
     3841 ggttttactc ggttacgtgg cggtcaaaac aaatgtgttt ctggggatga gcctgctgtt
     3901 tattttcgct ttcgggatgg gaacgctgct gattatcgcc ggcacgtttg ccgggatgat
     3961 tgccgctttg ccgcgatcgg gagcatggat ggaaaaaatt aaattcatat ccgggctgat
     4021 attaattggc gctgcggaat atttcctgta tattgccgga agactgtcta tataaaaaga
     4081 ggtgatgatg aacaaaaaaa ttttactggc aatcgctctg gtgtttgttt ttgcgttggt
     4141 tgctttctct tttgaacagt ttaagccgag tgtttttaca agacgcgaca aagtttcggc
     4201 tcctgatttt tcattgaaag acatccatgg gaaaacattc cggttgagcg cccagcgggg
     4261 aaatccggtc attatgtttt tcggcacgac ttggtgtccc gcctgccgcg aggaaatgcc
     4321 gctttacagg aacctttaca ataaatatgc cggacgcgga ttaaagttcc tgtatattga
     4381 tatcaacgaa tccgccgaac gggtggcacg gtttgccgga caaaatgctt ttccgtacca
     4441 cgtgctggtg gacggagacg gcagtgtagc cggcgattat aatattatcg gcgttccgac
     4501 cctgattctt ctggacagag agggcaatat cgtcgatgtc ggtcaccgaa tatctgactt
     4561 gccggtggac aggctttttt cggtaaaaaa ataaacagaa ttccgggggg aatgaaaatg
     4621 aacgagcgca actggctgca gaatctcaca agatcaaaca ccgataaatg gatcggcggg
     4681 gtgtgcggcg gtctggggga aagttctccc gttccggcgt gggcgtggcg tctgttgttt
     4741 gtgacgcttg tgttatgttt cggcacaggg gttctattgt atatcctgct gtggattttt
     4801 gttcccgaca aatcaacgat acaggagtaa gcatgagatt aaaaaacgat atttatcttt
     4861 acgagtggac caatccttat gaaaacaact gcaacagttt ttacgtcggc ggcggcgtca
     4921 atgcgctgat cgatccgggt ctgaccggct atgtccccga tttgctcgac cggatgtccg
     4981 ctgacggcat tcagaaagaa gatatccgct acgtcatcaa tacccattcg catcccgacc
     5041 attttcaggg gtcggaattg ttcgatcagg aaaaagtcaa ggtcggtctg cacaccaggg
     5101 agattgaatt tttaaaaggg gatggaggag agctctacag cctctttggg atcaacgctc
     5161 cccggatgca cgtcaattta ccgctggaag aaggagatct ggcgctgggc gatcagattt
     5221 tcaaggttat tctttccccc ggtcattctc ccggctccat cggtctgtac tggcccgacc
     5281 agaaaacgct gttctgcggc gatgtgatct ttgatcaaag cgtggggcgc acggattttc
     5341 ccggcggcaa cggcgatctt ttgaaaaaaa gcattctcgc cttcgccaag ctggatctgg
     5401 aatggctcct gcccggccac atgggcattc tgcacgggaa aggcagcatt cagaacaatt
     5461 tcaatctgat tatcaagggc atcttccctt acatataaat cctctatcac ggagaacgac
     5521 ctttttatgg ccaacgaagg aaacaaagtc atttactcca tgatcggggt aagcaagatc
     5581 tacgataaaa aaccggtgct caaggacatc tacctgtctt acttttacgg ggcgaagatc
     5641 ggcgtcatcg gtttgaacgg ctcggggaaa agctctcttc tgaaaattct ggccggtgtg
     5701 gaccaggagt ttttgggaaa aaccattctt tcgcccggct acacggtggg ctatctggag
     5761 caagaaccgc atctggacaa cgataaaacc gtgcggcaga ttgtggaaca gggcgtgcaa
     5821 agtacgatgg atctgattca ggaatacaac gacatcaacg aaaaatttgc cgaaccgatg
     5881 tccgatgacg agatggccaa actttgcgaa agacagggcg ccgtgcagga aaaactcgat
     5941 gccctggatg cctgggacat cgattcccgc ctggaaatgg cgatggatgc ccttcgctgc
     6001 ccgcccggcg acacatccgt gaaagtgctc tccggcggtg aaaaacgccg cgtcgcgctg
     6061 tgccgcctgc ttttgcagaa accggacatt ctgctcttgg atgaaccgac caatcatctg
     6121 gatgccgaat ccgtcgcctg gctggaacat catctgcaaa aatacgaagg cacgatcatt
     6181 gccgttaccc acgaccgtta ttttctggat aatgtggccg gctggattct ggaacttgac
     6241 cgcggtcagg gcatcccctg gcagggcaat tattcctcct ggctggaaca gaaacagaac
     6301 cgtctgaaaa atgaagaaaa ggctgaaagt gacagaatca aaactctcga gcgcgaactg
     6361 gaatggattc gcatgtcgcc aaaaggaaga cacgccaaat ccaaggctcg tatcagcgct
     6421 tacgaagaac tgctgggcaa aaccatggaa aaagaatccg gcacgcgcga aatcttcatc
     6481 gcgcccggac cgcgtctggg agatctggtg attgaagcca aggatgtgaa caagggctac
     6541 ggcgataaac tgctggtgga gggcatgacg ttttcattgc cgcccggagg cattatcggt
     6601 gtcattggcc ccaacggggc aggcaaaaca gctcttttca gaatgatcac cggccgggaa
     6661 aagccggatg ccggcacgat tcgcatcggc gatacggtga aactggccta tgtcgaccag
     6721 agccgcgatt ccttagatcc caataaaaat atctgggaaa tgatttccga cggacaggat
     6781 attatcgcta tcggtaatcg tcagatcaat tcccgcgcct atgtttcacg ctttaatttc
     6841 tccggaacgg atcagcagaa aaaagtggga acgctctccg gcggcgagcg caaccgcgtg
     6901 cacctggccc gcatgctcaa ggaaggcggc aatgtcctgt tgttggacga accgaccaac
     6961 gatctggatg tcaacacctt gcgcgcgctg gaagacgcgc tggaaaactt tgccggatgc
     7021 gtcgtcgtca tcagccacga ccgctggttt ctggacagaa tctgcacgca cattctggcg
     7081 tttgaaggcg acagcaaggt gctgttcttc gacggcaact attccgaata cgaggaagat
     7141 tacaggaagc gatacggcaa tgccgccagt cagccccatc gtatcaagta ccgccagctt
     7201 accagaaggt aagcgggaag gaccattcga tttgcatcaa tccgttgcag atgccccgat
     7261 ggttatcctg tagcaatctt ccggcttccg gttatttctt ctgaggtttt gattttatta
     7321 caagtttcac ctgacttcac agacggaaaa acccgattta cagatcttgc ggcacttaac
     7381 ttgaaatatc tgaatctcat taaattcgcg ttgcagccgc ctgcagtcaa taatacaata
     7441 cccaatcatt tcaagtacat ggaaattatt tcttgacata atcgtcataa taagtcaata
     7501 gcacaaacaa ttgttcaaag gtcaggagtg aacttattga ataaaaagcg tttagtttta
     7561 ctggttattc tctttattac gatcgggtta ttttctttgc agcagccttc ggagaattct
     7621 tttcgggggg atgccgttgt atcggaacca accgctccgg aaaagggaac caggccgtta
     7681 aaatccataa gcgacaaatt cgaaaaggga gaaacacttt cctcgatttt taaaaaatac
     7741 gggatgcaca ccgatgatct tttcgaactg aaaagtgcgg tggccagcat tcagtcgctg
     7801 cgggatatta agcccggcaa gccttacagt tttacgctca atgaagataa ttacatcagc
     7861 tcttttacgt gcggcatcga tgacgacacc attttaaaaa ttgaacgggt ggacggatgt
     7921 ttccaggcgc aaaaatataa cattccttat gagacccgta ttctgaccat cggcggcacg
     7981 attcaaaaca cgctggtttc cgcgttcggc aacgagcggg aagattacct gctggccctg
     8041 caggcggcgg acattctggc ctgggatatc gatttcaaca ccgatgtgcg caaaggagat
     8101 acattcagaa tcattgttga aggcctgtat tcggaaggga aattcaaaaa atacggcaga
     8161 attctgtcca tcgaatttgt caacggcagc cagacctaca acgcgtatct tttcgaacag
     8221 gacggcaaga cggattatta caatgccgac gggcaatcgc tgcgcaaggc ctttttaaag
     8281 gcgccgctga gtttccggag aatcagttct catttcagca gaagtcgcct gcatcccatc
     8341 ctgaaggtgc gccgtcccca tcacggcatt gactatgttg ccgccacggg aacgccggtc
     8401 agcacggtgg cggacggacg gatcacgttt gcgggttata aatccggtta cggtaaaatc
     8461 gttatcgtta accacgccaa cggctatcag acgtattacg gtcatttgtc ccgcatcaaa
     8521 agcaatattc gcccagggaa aaaagttcag cagggtgacc tgatcggaaa tgtgggagcc
     8581 accggtttgg ccacgggacc gcacctgcat tttgaaatga gacagggagg cagggcgatt
     8641 aatccgctgg cggttaaaaa agttgccggc agtcctgtcc cagcggcaca aatggccgaa
     8701 ttcaaaaaca caactcaaac catgaataat atttataccg tcgccctctt cttcgatacg
     8761 aataacacgc aacggaaaaa tcacagcctc cagccggcgc tcttgtatgc ttccctggcg
     8821 tcctcgtctc cgcagggcaa gtttcgtaaa aactgacgcg ggatcccttc agaaaccttc
     8881 cgcctgcttt atgaaatacc tgccatttag tgttttgttt tgatggtgcg aaacgtcaat
     8941 aatgccgcca ccagagcagg aataatcaga atcgccatgc cgatattgac aaagttctgt
     9001 gcgggaacaa catcagaaaa atagagaatc aggatcaaca gcgcgcaaac catattgccg
     9061 atgataaata cctttccgaa ataagcgttg gcttcatacc agacaaaatc attttccagc
     9121 gtggctttaa tgcgaaaacc gtaaatcaca tttttcggca ctttgcgtag cgccagagga
     9181 acggcaatca tcacgaaaat aaaattgcag atcaggacgg ttatcagtgt tttaaacggc
     9241 atgtcatatc tccttcatct gagaatgtcg ttgctcatcc gctttgaata attctttatt
     9301 ttttaaaaca catcggctag ataatcaagc gacgaaatac atgaggtctg tttttgaata
     9361 atacaaaaat tcccacggtc aagccgtttg ttcatcatct gcgtccgcgc ggtttatcca
     9421 ggcggattcc cctgatcgaa acattgcgaa actacaggcg tgaatggctg atgcacgaca
     9481 tccttgccgg tctggtgctt acggccatcc tggtcccggt cggcatggct tacgcatcgg
     9541 tctcgggatt gccggcgata tacggattgt atgccacgat cattccgctg attgcttacg
     9601 cgattttcgg acccagccgg attctgattc tgggacccga ctcggcgctg gccggcctca
     9661 tcgcggcggc catcctgccg ctggccgccg gcaataccga taaggccatc gcgcttgccg
     9721 gcctcttggc aatcctcgcg ggagcttttt gcattctggc cggtcttgtg aagttcggtt
     9781 tcattactga tctgctttcc aaaccggtcc gcatcggata cctcaacgga atcgcgctta
     9841 cggttctgat cggccagttg ccgaaagttt tgggttttcc cgtcggcggc aataatattt
     9901 tgcaggaagt taacagcctg atacagggca tcgccggcgg tnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnnnnn ncggcaataa tattttgcag gaagttaaca gcctgataca
    10081 gggcatcgcc ggcggtcaga tcaactggat gtcgtgcctc atcggatgtt cctgcctgat
    10141 gattattctg ggttgccggc aatggttctc ccgcattccg ggggttttca tcgccgtggc
    10201 cggcgcaacg atcatctctt cctggtttca tttatccgca agttacggca ttgccgtcgt
    10261 cgggccactg ccgcagggac tgccgcaatt ccagattccc tcggtgagct ttgctgaatt
    10321 taaagcgtta tttgctgccg cgttcgccat cgcgctggtt tcctttgccg atatgagcac
    10381 actctcgcga acctttgcac tgcgtgccgg tcaggaagtg gacagcaatc aggaaatcat
    10441 cgcgctgggc gcagccaata tggccgccgg attatttcag ggatttcccg tgaccagcag
    10501 cgcctcacgc acaccggtgg ccgaatcggc gggagccaaa actcaagtga ccggagtagt
    10561 cggcgcggta tgcattgccc tgcttctgat tttcgcgccg aatctgctca agaatctgcc
    10621 ccaggcggtt ctgggcgccg tcgtcatctg cgcttgcatc agcatcgttg aagttcgcgc
    10681 cctggcgcgt ttgtataaat tgcggcggga agaattcgtt ttctctatcg cgtgttttct
    10741 gggcgtggtt tccctgggga tcatcaaggg tatttttatc gccatcggcc tggcgctgtt
    10801 ttccttcatc tggcgggcct ggcaccctta cgatgccgta ttgggacatg tcgaaggttt
    10861 aaaaagttat cacgacatat cccgtcatcc cgatgccaga ttgattcccg gcctggtcct
    10921 ttttcgctgg gacgcgccgc tgtttttcgc caatgccgaa gccttccgcg agaatgtgct
    10981 tcatgccatt gccgaagcgc cgacacccac caagtgggtt gttgtaacgg ccgaaccgat
    11041 taccgatgtg gacaccactg ccgcagacat gctggccgaa ctggataatg ttctgcacga
    11101 atcgggcatg gcattatttt ttgccgaaat gaaagacccg gtcaaggaca tgctcaagcg
    11161 ctacggactt tttacacgat tcggcgagga gaatttcttt ccgaccattg aacaagctgt
    11221 ggaacgctat ctgacactgc acaaaatcga ctggccggaa tcgcataaac gatagcgaaa
    11281 acattatatt gattgcttac ttcaaacagg aaatattgac cggcatatga atctttgccg
    11341 ctgtgtttta tcctcagtaa atctgacatg agcaaggtaa aggattgccc cttttctttg
    11401 tcattccgtc gtaggccgga atccagtttt tttctggatg ccgttatgca acggcatgat
    11461 agcatctgtc ttttcgtatc cccgaatgaa cttgtcgggc tatggtgacc tctgggtaga
    11521 tccagcttta tttccaagtt aaaccgagtc ttttatcgcg ggatgttgtc ccaatccttt
    11581 tttcttgaca atgtttaatg ctcccggctc attggaaata atccagtttt ccagatcaaa
    11641 tagaattttt tggacgcggg gcagtttcat gagttcccgg atcatgcctt cgataacggg
    11701 aaggctgaac tcgccctggg actcccggac gagattgccg gacatataga ccgttctatg
    11761 gacaaaggaa tagctgattt tcgtcatgtc ggcattgtgg ctgacgagaa cctgcctgat
    11821 ttttcggttg atctcatatc gaagcaattc agacgacatg ggcactttca gcgatgttgt
    11881 gttttgtatt gctaagacag cttaattttt cttctttccg gattcgggtt ttgtcgatag
    11941 aaaatggcaa tgttgccgat caggccgaca agttcgcttt tggttttacc ggcgatttcc
    12001 tgcgagattt cttttttagc ttccttgaat tcgccgaatt ttatcttgat gagttcgtgg
    12061 tcgtttagag cagcgttaac cgagctgatc agttgcgttg tcgagccttt ggcgccgatc
    12121 atgacgaccg gctttaagtg atgcgcctgt gcgcgcaaat attttttttg cgatcctttg
    12181 agtttctcca tgatttttcc tttcaggatg gaacatcatt accacaaata accctttatt
    12241 gtaagactca aatttcaaaa acgcagtgta ttgaaatttg taagtcgaac aatctcgcta
    12301 taagcggagg gtatcatacc ccataccttg gggcggaatc atggttcatg gtttgtattg
    12361 gggttcatac ccgtgattaa aagactttac aaaattcctg tatcttgatt aaagtacaga
    12421 cactttattc agggggtttg ggacgtgagc aaagcaaatt taccgcaata cgattgcgat
    12481 acgataatta tcggcggcgg gccgggcggt tataccgcgg gcatttatgc cgcacgcagc
    12541 ggtctgaaat cgctgctggt ggaaggtgcg tcaacggtca gtcagataac cattaccgat
    12601 ctgattgaaa actatcctgg cattcccgac ggcatcaacg gtttcgacct gatgcagcta
    12661 ttcaagaaac aggcgcagaa tttcggcctg gaaatcatcg ccaatgatgt tcactccatc
    12721 aaaaaagccg gcgacaatcc ggcgatctgg gaaattgccg tgggcgataa atcctacagg
    12781 acgttaagcg tgattgccgc caccggcgcg cagtggaata aattgggcgt tcccggcgaa
    12841 gaagaatttg ccggcaaggg cgtatcctat tgtgccacct gtgacgggcc tttttaccgc
    12901 aacagggatg tcgtggtcgt gggcggcggg gataccgcca ttcaggaagc cctgttcctg
    12961 actcactttg ccaaaaaagt ttccgtcatt caccgccgcg accgccttcg cgccgcggcc
    13021 atcctgcaaa aaagagcttt tgcggaaaag aaaattgaat ttatctggaa ggcaaaactt
    13081 gccgagatcg ccggtcagga gtttgtcacc ggtgtcaggg tggccgatgt ggaatcaggc
    13141 aaaattaatc atatcgaagc ggaaggtgtt tttatttttg tcggattgac tcccaacacc
    13201 gctctcttcc gcgacgtttt gaatctggat aaaggcggtt acattatcac ggatgacaac
    13261 atgcgcacat ccgcacccgg catatttgcc gcgggcgact gccgtgccaa acagttccgt
    13321 caggtggtca cagccgcggg cgacggtgcc aatgcgattt acagcgccga actatatgtt
    13381 gatgaactca agggccagac atactaaact gtcaccatat cgaacgcaga gtgtagtccg
    13441 gctttgccgg gaagatatct aaaacacaag gcttctcctc ggcatgcgcc gggtcgaagt
    13501 gacaaaatct atgaattagg cagacagaaa cacaacatgg aagctttcat caatacctgg
    13561 cagcaattac cctcgcatat taatcccgct cttttcacca tcggttcttt tcaattgcgc
    13621 tactacagcc tgatgtatct ggttggattc gccatcgttt atctgctgac cacttatcgc
    13681 ataagcaatg aacattacga atacaagaag gagatgcttc aggactatct catctggggt
    13741 atgttcgcga tgatcgtcgg cgcgcggctg ggcgaagtgt taatttacaa tttttcctat
    13801 ttttctcagc atccgctgga gatttttctg cctttcagct tttccaacgg aattcatttc
    13861 accggtatca gcggcatgtc ttttcacggc ggcgtggtcg gcgtaatgat atattcgatc
    13921 tattttctgc gcaaacgaca gatcaacttc tggcggttcg ccgatttgat tgttccttcg
    13981 attccgctgg gctacacgtt cgggcgtctc ggcaattttc ttaacggcga attgtgggga
    14041 cgcgtaacaa cgatgccctg gggaatgtat tttccttttg atccgacgca gcatctgcgc
    14101 catccgtccc agctctatga agccttcttt gaaggaattt ttctgttcgt cattctgtgg
    14161 ctgttaagaa agaaaaagtt tgccgacggt tttcttctgg ggctgtacat tttcggctac
    14221 ggttttgtcc gtttcatgat tgaatttttc cgcgaacccg actttatggt cggttcggtc
    14281 agtatcgggc aggttctttg tgtgttgatg atggcggccg gactcattat tgttttatgg
    14341 cgcagacaaa ttgcgtcacc agccaggtaa cattggaggt cgtatgggaa aacatttaat
    14401 tattgtcggc ggaggagccg gcggagcgtc cgcggcggct gaagccaaac gaaatgatcc
    14461 agaactgaat gttgcgattc tgcaatcggg aaaattcatt gcctacgcgt cctgccccac
    14521 accttactat atcggcgaca ttatcaaaga tgaaaataga ctagtggcgc gcaccccgga
    14581 agaattccgt aaacggggcg tggaggtgca gcttgacacg gaagtcgtca atattaattg
    14641 taatgcaaat gttgtcgaaa caagggacaa ccttatgctg ccgtatgatt atctggtcat
    14701 cggtaccggc acgaacccct ttgttccgga cattcccggc cgggaactgc caggtgttta
    14761 tgtactgaac aatctggaaa atgccattca cattaaagcc tggctgcgtg aaagaaaagc
    14821 caagcgcgtg gttattatcg gcggcggttt cattggcctg gaaatgagcg aagcgctgca
    14881 cgaagtggga atttccacaa ccatcattca caaacattct ttacctgcaa atcgctggag
    14941 taaagaacta tcgtcggtga tgctcgaaga acttcagtcc cacagcgttg aattcatcgg
    15001 caacgcccag gcaaaagcca ttgaaaaatc atccggcgat tctttgaaag tgattaccga
    15061 cgcgggcgag tggcagggcg atatggtgct gctggccgtt ggcgtccggc ccaatgccgc
    15121 gctggccaaa tctgccggtc ttaccatcgg caaaaccggc gcgattgccg ttaatttcgc
    15181 ccagcagaca tccgttgaaa acatttacgc cgtcggcgac tgctgcgaat cttggaaccg
    15241 cgtcagccgc cgctgggtca atattcctct gggcgacatc gccaataaac aggggcgtgt
    15301 tgccggacgc aacatcggtg cgaaaccgtt gacgttcgac ggcattgtcg gcgcacaatc
    15361 tttccggctg tttgatctgg aatgcgcggc cacgggtctc tcggaaaaag aagcgcttgc
    15421 cgcaggcttt gtgcccatca gcaacatcac atggggcagc gccatggcac cggcaatggg
    15481 catgaaaaaa atagggctga agctgactgc cgataaatcc tcgggaaaac tgctgggcgc
    15541 gcaggcggtg ggtgttgccg gcgcggtggg acggatcaat gcgctgtccg tcgctctctg
    15601 gaccggatta aatattgacg aagttggtta tctggatctg gcttacgcgc cgcctttcag
    15661 cgcggcgtgg gatattattc acaacgctgc tcaggctttg cgccggatga tctgaaagga
    15721 aaaggactta tcaatgaccc tctggtggac gatcttcggt gtctttatcc ttgcgatgct
    15781 ggcgttggat ctgggcgttt ttaaccgcaa ggctcacgtc atcaaaatga aggaagcgct
    15841 cctatgggtt tccttctggg tatcccttgc tgttgcgttc ggtgtgggtg tttattattt
    15901 ttacggccac tccaaggcgc tggaattttt taccgcttat atcattgaat actctctgag
    15961 catcgataac ctttttgtgt tcctgctgat ctttcggttt ttcaacgtgc cgcaagccta
    16021 tgaacacaag gcccttttct ggggcatcct gctggcgctg attacccgcg ccgcgttcat
    16081 ttttgcaggt gtcgcgctca tcaacaaatt ccactggatc atgtatcttt tcggcgcttt
    16141 cctgatttat accggcatca agctggcgat gaacaaacaa acggaagtcc atcctgacaa
    16201 aaacatcgcg ataaaaatgc tgcgctatgt tgttccggtg accaaagaat ttcatgaagc
    16261 caggttcttt atcattaaaa acggaaagcg tttcgccacg cccatgctgg ccgttttgct
    16321 ggcgctggaa accaccgata ttttgttcgc ggttgattcc attccggcgg tactggcgat
    16381 ttccagagat ccctttatcg tttacacctc caatgtcttt gccatcctgg gactgcgttc
    16441 gcttttcttc gccatttccg ggctgatgaa actctttcat ctcctgcact atggcctttc
    16501 agccattttg agttttgtcg gcgtaaagat gctcattgcg gatttttacc atataccggt
    16561 gggaatctct ctgacggtca tatcgtccat tctcgtcata tccattgtcg cgtccatcat
    16621 ctggccggat acaaaatatg aagacattcc gaacatcacc atgaaggatt aacgccatgc
    16681 aaaacggatt tttgacgcct tcacagattc agttgaaaaa atgggccaga ctggacgacg
    16741 cgaaggcgcg ccatgaagaa ggggtgtttc tggccgaagg cgtcaaggtc gtcgaagaac
    16801 tgctgaaaag cgactggcaa attaaagcat tgctggtcat gccggaaaaa ataaaattct
    16861 ggaagaagct tgatctttcc cgggaggaag aaattcccgt ctatcagttg aagcgaacac
    16921 agtgggaaaa aatcggacag gaccgggaac cggaaggcat catggcgctg gtagaaatga
    16981 aacaggcgcc ggatttttcc tcgtggcttg cacaaacttc cggcaatgtt ttgatcctgc
    17041 atgaaatcaa caatccgctc aatctcgggg cgctggcccg cagcgcgcag tggtttggat
    17101 ttaacagtat catactgagc gccaactccg tggacttcac caatccgaaa gccatccgtt
    17161 cctcgatggg cagccttttt catttgacga tgattccgga gctggattta atggaaacgc
    17221 tgccggaaat cagaaaacat ttttttatca tcggttccga cgttcatgaa ggaacagcgc
    17281 cgcatccggt gcagaaaaat gccgcgctga ctttaggcaa tgagagtcac ggcctgccgg
    17341 cgacgatttt aaaaatgacc gatgaagaat ggagcatccc cggcagcggc aaagccgatt
    17401 cgctgagttt gccgcaggca gcagcgatca tgatgtacga atgcacaaaa aaataaaccg
    17461 aagcaaacaa tattgttatg cctgggaatg ccggaatccg gttttgaagt atattataga
    17521 tttccgaggc attcgccggg caggcttcct gcccgtaaag aactatcttg acatgtcatt
    17581 accatgaact ccagccgtcc atcttgacct ggcgcttgta tttgctcaat ttcacacatc
    17641 tgtctatatc aatgaagcgg ggcagaagct ctatatgact tttatgaaga taatagcggt
    17701 taactccatc ggacatcgca aacacataat tattgttcag aacgatctgt tcccaaagca
    17761 tataggaaag atctcccgtg cccggacgcg acgcttcaat acacaacacc acgggcttat
    17821 atcgcgcaag atcaatactc tcgagaacct gtttctcaaa gccttcaaca tcaatggtga
    17881 tgaatgtaat ttcacgaacg gatatctttc caagcaaatt attaagtgtc gtgacaggaa
    17941 caacaatttc cttgaaaaga attccgtttt tcttttgaat atccacgatt tccttatcga
    18001 aggttgaaag cggcatcaat gcatccgatt tgtatgcctg ctgataaaaa atcatacgtc
    18061 cttcagttgt ggcaataccg acattataat tgatatcgcg ctgccgaaaa gagcgaagtt
    18121 gtaaatacaa ctcggcattg ggctcaatat tgatgccatg ccagcccctt tcatagaaga
    18181 atcgagttac attaaagata ttgggatcgt tagctccgac atcgatatag aaaccattgg
    18241 cgacatcctg aaaaacatgg gcaagtatat aatcctcata agtctggctg taataaatcc
    18301 cgttttcaaa tttagaagga ggacaatcgc acgccccgtt ttgttgccgg caattgacca
    18361 gagaaaacat aacggaaatc gcaaggcaca aaaaaacaag gattttaatt tttgatttca
    18421 ttgttttccg cctcgcgtaa tttgtttttt atgatttcgg atggaaatat cttgcgacaa
    18481 atcatcaaaa aataccatct tttatttttc tgaaattcat gccttttcac gattccatga
    18541 ataatatgaa accggatcgg tgtaattgcg aaaacaattt ctttgttgtt tgttttttct
    18601 tgatttcgta aggtcaaaca aataatatgc tatccaatgc aaataatgac agatttgttc
    18661 atcgtccaaa gccgattcgc tgagtttgcc gcaggctgcg gcgatcatga tgtg
//
LOCUS       DAOOLB010000037        18239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_3155_length_18239_cov_41.741806, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000037 DAOOLB010000000
VERSION     DAOOLB010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 18239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18239
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3155_length_18239_cov_41.741806"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLF77_06225"
     CDS             complement(<1..590)
                     /locus_tag="PLF77_06225"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPC08484.1"
                     /translation="MKKQRKHCLYCGGKTVKKYEDNVRRDFCPVCNSYFYENPLPVVS
                     SILEDSRQILLVKRGKRPSKGLWCLPTGFAEAGESIEEAALRELEEESGIKGKIIKLL
                     DVDSYKSRFYGDLLFLTFVVQQTGGKLCAGDDCSQARFWPVHKLPPLAFRSNKRALDA
                     YIKSRKDYWAIFDSIEHADKKSLPPAVAKNSLTRRLV"
     gene            complement(587..1447)
                     /locus_tag="PLF77_06230"
     CDS             complement(587..1447)
                     /locus_tag="PLF77_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC08485.1"
                     /translation="MSDILDKKKIRKVFRNVQKHLSIAQLIRRFSTNKEDIRKAALNG
                     VNLSRCRSVLELGCAFGAFTEALRNKLHDGATITGVDIISEYKPFFLDACRRAGYEGT
                     FLSSGIEKIKKIPSGFFDLVICSYALYFFPEIIPEIARIMKPSGFFITITHSSNDMRE
                     IVDIVKRILGRHHASGTNRSLPIEIIFDQFSAENGESLLQPFFKTIEAIDFKNTLVFQ
                     SQEIRYFMDYFQFKKSFFLSEQDANNQTIIRQLFKELQETLSKNQIVSMCKDDRIFIC
                     SQPSTGKDSP"
     gene            complement(1480..1920)
                     /locus_tag="PLF77_06235"
     CDS             complement(1480..1920)
                     /locus_tag="PLF77_06235"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HPC08486.1"
                     /translation="MKKIVIASDHGAVALKSEIIAFLKTKQCELKDLGVDSDDPVDYP
                     DIAVLTCTEFKRGGYDFGILLCGTGIGVSMTANKIKGIRCALIHDLFTAEMAKAHNDA
                     NFIAFGGRVKYNVPVTKMIEKFMETEFAGERHCRRVGKIMDAEK"
     gene            2037..2927
                     /gene="xerD"
                     /locus_tag="PLF77_06240"
     CDS             2037..2927
                     /gene="xerD"
                     /locus_tag="PLF77_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPC08487.1"
                     /translation="MRALLMKYLDYLFIERGMAQNTLESYSRDLHRYLAFVQTNRKIS
                     DIDNVTPEIVVDYLTKLREEGLAANSMNRALAALRGFYKYLLREKVIAQNPLAHIELA
                     KVWMKLPETISKEEMKTILAQPGNRNNAAIRNTAMLELLYATGIRVSELINLTMNSIN
                     WQVGFLIVMGKGSKERIVPIGRIAYDCVRRYVDEIRPKFMQKKSTDVLFLSRFGKKFT
                     RQGFWKIVVACARQAGLQKKVYPHTFRHSFASHLLEGGADMRTVQVMLGHSDIATTQI
                     YTHITRERLKEVHRKFHPRA"
     gene            2983..5001
                     /locus_tag="PLF77_06245"
     CDS             2983..5001
                     /locus_tag="PLF77_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC08488.1"
                     /translation="MIHQDRQDRRPKLNGRLPVKTLSIAAAVVVCIAVVALLFYQGFF
                     LSLAFQMSRIKNTFSYYVLKEKPQFYYLDVEKNGQDMRIYRNDVLEITYRDEVVVKAV
                     ASDDLSGKYTYVRFEGLSSRSSDLGVLMKGIDFVNKLIRTGGMSGRSGTVNNYQILVH
                     YLNDTIASVPLKIVITPQDWFRFAKESSNVEQQIDYLKKAIELNQNDVRVRKILAGIY
                     AHHGRTDDAKKLYQQVLTIQSDDTTAMLELVKIHIKQKEFGAAAKLAGDLIKINPKET
                     EGHLALGLALEEKKAWSKAAQHYREAVRLEPHNDAARFRLADAYKNGNMMSNAIDEYQ
                     YIVKHSREPEEALFALGDIYLKLKKTDEAIKCYREILRRQPKNPVAHANLAVAYAGSG
                     KAQEELQSLKKAAALNPNEPTIRFNLGAVYERKKMYGDAAREYRHVLKINPSDTDALE
                     RLADLSLKNKQYSQAVNYYERLKTKSSRKASVYANLGFAYGELKKYQAAASNYEKAIQ
                     LGAKEPVLRANLADTYEKMGMKKKAIAVYEKMPASSKDASTALADLYLKEKNYQKAIA
                     IYQKLAKQEPKKAGSYANLGFAYAAAGNYDQAIKHYNTALKFDSDDADIYDNLGEAYE
                     KKGLYQKALETYKKAHALNPESPKAAQRIPRLNIQLLQERKRQNTGSE"
     gene            5004..5843
                     /gene="kdsA"
                     /locus_tag="PLF77_06250"
     CDS             5004..5843
                     /gene="kdsA"
                     /locus_tag="PLF77_06250"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HPC08489.1"
                     /translation="MKKIKMGNVTIGDNKKFILIAGPCVLEDEKKAMAIALYLKKLTE
                     RLKMPFIFKASYDKANRTSIKSFRGPGLKKGLAILQRIREKLGVPVLSDVHRFEEIDP
                     ASKVLDVMQVPAFLCRQTDFVTEMAKKARVINVKKGQFLAPWDVANIIEKARAAGNDN
                     IMITERGATFGYNNLVFDVRSLPIMRKMGYPVIFDATHSVQLPGGRGASSGGQRDMVT
                     YLARAAVAAGVDGVFFEVYPDPEKALCDGANSLYLDSLEDLLVTLMKIDALVKTPSNG
                     KNQ"
     gene            5866..6399
                     /locus_tag="PLF77_06255"
     CDS             5866..6399
                     /locus_tag="PLF77_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HPC08490.1"
                     /translation="MKKNLKEKLKKIKLLMLDVDGIMTDGRIIMNDEGCETKHFNVRD
                     GHGLKILQRFNIKVAIITGRQSKVVSHRARDLDITDVYQKVYNKKEVFEKILKKYKLS
                     PDETAFMGDDIVDIPVLNRVGFSATVADASDVVKKQVDYITGHKGGKGAVREVCEMIL
                     QAQGKWAEIAARYEFLK"
     gene            6525..7082
                     /gene="lptC"
                     /locus_tag="PLF77_06260"
     CDS             6525..7082
                     /gene="lptC"
                     /locus_tag="PLF77_06260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPC08491.1"
                     /translation="MIKNRKKTIVFFSVVTVFLAVFLAAFLIFRDSINKPKTLLKALQ
                     DNVDLQIKGFVYTEVGENKGKWEVKAASASYDKKQNLAVLEQVRVTVITADGKKYEMK
                     ADKGRIFTDKKNMNISGNVVITSDDGDNFFTEYVQYSDEERKFFTDAPVRMESRRIRI
                     TGQGLAIFLNSGELSIPSMVKANIH"
     gene            7090..7653
                     /gene="lptA"
                     /locus_tag="PLF77_06265"
     CDS             7090..7653
                     /gene="lptA"
                     /locus_tag="PLF77_06265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HPC08492.1"
                     /translation="MIRKHTYIFLMLFFFFCYVEICPGKNTLTARTDKPIQITAARME
                     AFKNRSQIVFSGNATVVQGDSVLKAEKLILHYRQQPKTEKAQKARDNQAGDLEKIEAR
                     GNVSVVQGTRKATADEAVYVQAENKIIMTGNAELKEGKSSIRGERVIILLDEDKGIVE
                     GGERMRVKAVIYPQERRQTENQSTMKN"
     gene            7663..8385
                     /gene="lptB"
                     /locus_tag="PLF77_06270"
     CDS             7663..8385
                     /gene="lptB"
                     /locus_tag="PLF77_06270"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPC08493.1"
                     /translation="MSDLSAKGLTKIYNKRKVVNQIDLLIAPGEVVGLLGPNGAGKTT
                     TFYMIVGLVKPDAGEIYLDGENITGNPMYRRARKGLNYLPQEPSIFRKLTVEENILAI
                     LETLDLSEAERQERLRELLKELDLTALAKNYAYSLSGGERRRVEITRALVTSPKYILL
                     DEPFAGIDPLAVADIQKIIEKLKAKGIGIIISDHNVRETLSCCDRAYIVNNGEILVEG
                     PPDTIAQSELARKFYFGDSFNL"
     gene            8409..9893
                     /gene="rpoN"
                     /locus_tag="PLF77_06275"
     CDS             8409..9893
                     /gene="rpoN"
                     /locus_tag="PLF77_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPC08494.1"
                     /translation="MAFELKQHLKLSQQLIMTPQLQQAIKLLQLSRQELVEAINQEME
                     ENPLLEEISTDENSDEALIGEFENDGLPVERDTIKAVEHTEELNVEKGAGTDEFDWAS
                     YLEDYGPVGMTYGGKDGEETSWDNVLTEGQTLSKHLTWQMKLSSFSEDEERVGSQIIG
                     NLDPNGYLCATAQEIAQLENVSEELVERVLQKVQEFDPPGIAARNLQECLLIQARMLG
                     VKNRIIEVIIRDFLKELELKNYAQIAHKLKVPLKEVEMAVLLISEMNPKPGSIYSEDK
                     AQPIIPDVYVVKTGDEYKIILNDDGLPRLRISNFYREVMAGISSHSHEEAENGKKYIK
                     DKVQSATWLIKSIQQRQNTIYKVAESIVKFQKEFFDKGIDYLRPLILRDVANDIEMHE
                     STISRVVNNKYMHSPQGIFELKYFFGSSIRTTTQGTIASKSVQEEIRQLISSEPPRKP
                     YSDCEMVQLLKAKGIHIARRTVAKYREMMGILPSSKRKKYFKAI"
     gene            10154..10687
                     /gene="raiA"
                     /locus_tag="PLF77_06280"
     CDS             10154..10687
                     /gene="raiA"
                     /locus_tag="PLF77_06280"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HPC08495.1"
                     /translation="MMKISVTFRNGEGENWQKNYAEEKMQKLKKYLDAPAEAHVVVSM
                     EKFRNVTEINLNSNGWNINAKEEAKDMRVALDRCVEKIEKQLKKQREKIRQHKPKTIR
                     RAQEKPLNEEEIEEPTASKIMETRKVILKPMSFDEAIMEIEETNDRFVLYRDTSSENV
                     SMIYRGDDGRYTLIETK"
     gene            10779..11222
                     /locus_tag="PLF77_06285"
     CDS             10779..11222
                     /locus_tag="PLF77_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HPC08496.1"
                     /translation="MEFINAGLEAKNKDEVLAELVNVIIEGGLKLNSSSIIDILKQRE
                     NLGSTGIGEGVAIPHGKISNLNDIVVAFGRSEKGIAYDSLDGKPVHLFFLLLAPENSA
                     GQHLKILAKISKMLKDVYFRNKLIAAKSRDELYQIIIDQERTCIV"
     gene            11347..12213
                     /gene="rapZ"
                     /locus_tag="PLF77_06290"
     CDS             11347..12213
                     /gene="rapZ"
                     /locus_tag="PLF77_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPC08497.1"
                     /translation="MKNLRVVIITGISGSGKSTALRALEDVGFFCVDNLPVILLPKFL
                     AITLASSPEIKRVALVMDLRQRSFLDKYSRIFASLKKKGYKIEILFLESSDESLVHRF
                     SETRRVHPLLERGIIMEGISLEREKLSSLKKMADKVIDTTSFNVHQLKDVVQRSFSVF
                     SKNKRMVINVQSFGYRYGLPVDADLVFDVRFLPNPYFIEKLKNFDGNNRNVQNYVLRN
                     RESKQFLEKIKDLMNLLTPLYEKEGKVLLNIAIGCTGGKHRSVVMANKMSAFFTSLKY
                     HVNVNHRDVNKH"
     gene            complement(12241..13554)
                     /locus_tag="PLF77_06295"
     CDS             complement(12241..13554)
                     /locus_tag="PLF77_06295"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPC08498.1"
                     /translation="MKWFRFDIRILIALVVVAALGWFIFGNYIEIGNPVIKLDSDVKA
                     IGRQKTVGITFSDSKSGLSRITAEIIQDNKGHVLADKKIPQKGVAHESLSLNIASTQL
                     ALHDGPAVLKISATDHSLLKNQTVLSQSVTIDTIPPMINLLKNINYINQGGTGFVAYQ
                     PSKPLSSTGVYINDYLSPAHAVLIDNKPSYITYFALPMTADKAATSIMIFARDEAGNE
                     TRVSVPCVIKEKKFRSDKMHLSEAFLQRKMPEFQAMVPSLQGKTLLDVFTYVNSQMRK
                     ENFSAIQKICMKSAPKELWEGEFLRMSNAAPMALFGDKRSYAYEGKVVGESVHEGVDL
                     AANARSPIEASNKGIVVFTGALGIYGNTVIIDHGLGLFSLYAHLSSIDTTVGKTVAKG
                     EKIGLSGMSGLAGGDHLHFSIMVGGKFVDPKEWWDPHWIKDNITR"
     gene            13884..15095
                     /gene="coaBC"
                     /locus_tag="PLF77_06300"
     CDS             13884..15095
                     /gene="coaBC"
                     /locus_tag="PLF77_06300"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPC08499.1"
                     /translation="MLENKKIVLGITGGIAAYKAAELTRRLVKAGAHVKIIMTKSALE
                     FITPLTMQTLSQNRVYTDMFASDDEYDMAHIALAEFADAFVVAPATANIIGKIASGIG
                     DDLLSTTIMAENKPTLICPAMNDKMLANPIVQDNIRRLKKNGYAVMDSAVGELACKTE
                     GKGRLPEIDAILAEIENIMTPQDLAGVRILITAGPTEEPLDPVRFITNLSSGKMGYAL
                     ASAAQRRGARVTLVSGPTSLPLPPLEKIVTVRTAREMHKAVLQHYKSARVIIKAAAVA
                     DYRPKIVANEKIKKEGKTLFIELERNPDIIAEIGKKKGNRILVGFAMETQNLLTNARG
                     KLVKKNMDMIVANNLRQEGAGFRTDTNIITVIDRAGKTESLEKMTKIDAAGAILNRIK
                     KLISEKSKGKK"
     gene            15106..15825
                     /locus_tag="PLF77_06305"
     CDS             15106..15825
                     /locus_tag="PLF77_06305"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPC08500.1"
                     /translation="MSNITRNELQAELLHAVRGLKGQVIHNESLAVGVDWSRSSRTVV
                     AKAKTGGQSRGEMLEAIHQEMADCQLCPLGRTRKNLVFGTGNPGARIVFVGEAPGADE
                     DEQGIPFVGRAGQLLTEIIVKGMKLQRKDVYICNILKCRPPGNRNPQPDEISRCEPFL
                     KKQLQLIAPEVICALGTFAAKTLLKTDVPISVLRGRFHSYEGIKLMPTYHPAYLLRNP
                     AAKKQVWEDVKIIMKELNLPY"
     gene            15847..17154
                     /locus_tag="PLF77_06310"
     CDS             15847..17154
                     /locus_tag="PLF77_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HPC08501.1"
                     /translation="MKQIRYALMAVFIFAGLLAWAEEKNPIFNVITVDGVITSPTAKY
                     IASGIEEAVRDNAGGLIILLDTPGGMDTAMRDIAKSILNSPVPVIVYVSPSGARAASA
                     GVIITQAAHIAAMAPGTNIGAAHPVAIGLGNSGGEKDKTMTEKIENDAAAYARSIAKT
                     RGRSEEWVEKAVRKSESITAEEALKLNVIDYVAADVNMLLAAIHQKEIQLIGGKKIIS
                     TKNAVIRDKKMGTRQGILSAISDPNISYLLLLIGLAGLYFELSTPGAILPGVIGGISL
                     LLAFFGLSTLPVSYTGILLIIFGVILFIAEIKVMSHGMLTVGGIISLVMGSLLLFDTP
                     EPALRLSLQVLIPAVVVVSIFFIVIIMLAVKAQMRKHFTGAEAMIDLEAEVTSDIEKE
                     GDVFVKGEYWKAVSDASIKKGAKVRIVKVDGMKLVVEEIKNQK"
     gene            17184..17939
                     /locus_tag="PLF77_06315"
     CDS             17184..17939
                     /locus_tag="PLF77_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HPC08502.1"
                     /translation="MLGTLSMPTLVVIVLVVMFLVSAIKILSEYERAVIFRLGRIRDA
                     KGPGLIIIIPGIDKMVRVDMRTITMDVPPQDVITKDNVSIKVSAVVYFRVMDANSAVT
                     NVENYLYATSQLAQTTLRSVCGQMELDEILSEREKINMNLQEILDRSTEPWGIKVSLV
                     EVKHIDLPEEMKRSMAKQAEAERERRAKVINAEGEFQAAQKLIEAAALMETQPMSLQL
                     RYLQTLNQIASENNSTTVFPIPIDLFKPFLKDK"
     gene            17998..>18239
                     /locus_tag="PLF77_06320"
     CDS             17998..>18239
                     /locus_tag="PLF77_06320"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPC08503.1"
                     /translation="MSDKKQNVITGGTVLITLGILIYLSKSGSFPFGQTWPVLLIVIG
                     ICTLIQKFRDLGGWIITLAGTGFLFYEFYGFDVSQYS"
BASE COUNT         5289 a         3828 c         4695 g         4427 t
ORIGIN      
        1 accaggcgtc gcgtcagaga attttttgcc acggcgggtg gcagggattt tttgtccgcg
       61 tgctctatgg aatcaaaaat agcccagtag tcttttctgc ttttaatgta ggcatccaga
      121 gcgcgtttgt tggagcggaa ggctaaggga ggtaatttat ggacaggcca gaatcgggcc
      181 tgtgagcaat catcgccggc gcaaagcttt ccgcccgttt gttgaacgac aaatgtcaga
      241 aacagcaaat caccgtaaaa gcgactttta taggaatcga cgtcgagtaa ttttataatt
      301 tttcctttta tgccgctttc ttcttccaat tcgcgcaggg ccgcttcctc gatactttct
      361 ccggcttcgg caaatcccgt cggcaggcac cacaatcctt ttgacgggcg cttgcctctt
      421 ttaaccagaa gaatctgacg ggaatcttcc agaattgagg aaacaaccgg cagcggattt
      481 tcataaaaat aagaattaca aacgggacag aaatcgcgcc ggacgttatc ttcatatttt
      541 ttgacggttt tgccgccgca ataaaggcaa tgcttgcgct gttttttcat ggggagtcct
      601 tgccggttga aggttgcgaa cagatgaaaa ttctgtcgtc tttgcacata ctgacaattt
      661 gattcttcga tagtgtttcc tgcaattcct tgaagagctg acggatgatc gtttgattgt
      721 tggcgtcctg ctccgagagg aaaaaagatt tcttgaattg aaaatagtcc atgaaatatc
      781 tgatttcctg agactgaaat accagcgtat ttttaaagtc gatggcctct atcgtcttga
      841 agaaaggctg aagcaaggac tcgccgtttt cagccgaaaa ctgatcgaaa ataatttcta
      901 tcggcagaga ccggttggtt ccggaagcat gatgtcgccc gagaattctt ttgacgatgt
      961 caacgatttc ccgcatgtcg ttgctggaat gcgtaatcgt aataaaaaaa ccgcttggtt
     1021 tcatgattcg ggcaatttcc ggaatgatct caggaaaaaa gtataaagcg taactgcaga
     1081 tcaccagatc aaagaatccg gatgggattt tttttatttt ttcgattccg gaagatagaa
     1141 aggttccttc gtaaccggcc cgtcggcagg catcgagaaa aaaaggcttg tattcggaga
     1201 tgatatcaac gcctgtaatg gttgcgccgt catgcagttt gtttctcagc gcctcggtaa
     1261 aagcaccgaa cgcgcagccc agttccaaaa cactcctgca tcgggacagg ttgaccccgt
     1321 tcagggctgc ttttcggata tcttctttgt tggtggaaaa acggcggata agctgggcaa
     1381 tgctcaggtg tttctgaacg ttgcgaaaca ccttgcgaat ctttttttta tcaagaatat
     1441 ccgacatttg gattcctgaa tgaaaaagct gtctgaatat tatttttcag cgtccatgat
     1501 tttaccgact ctccggcagt gcctttcccc ggcaaattcg gtttccatga atttttcaat
     1561 cattttcgtg acgggcacat tatattttac ccggccgccg aaagcgataa agttggcgtc
     1621 gttatgggct ttggccattt ctgcggtaaa gaggtcgtga atcagagcgc aacggattcc
     1681 tttgatttta ttcgcggtca tggatacgcc gattcccgtc ccgcacaaaa gaatgccgaa
     1741 atcatatccg cctcgtttga attccgtaca ggtcagcacg gcgatatcgg gataatcgac
     1801 cggatcatcg ctgtcgactc cgagatcctt caactcgcac tgttttgttt tcaaaaaagc
     1861 gatgatttcg gattttaaag ctacggcgcc gtgatcggaa gcgataacaa tttttttcat
     1921 gagcggttcc ttgtcagtaa cgttttagag aggtttctgt tcctattttt atgatattca
     1981 gaaataagtc aataaaaaat ctttgcttca gaaagataat taagttaaaa agtcatatgc
     2041 gggcattatt aatgaaatat ctggattatc tgttcataga acgcggaatg gctcagaaca
     2101 cgctggaatc ctacagccgg gatctgcacc ggtatctggc ctttgtgcag accaaccgga
     2161 aaataagcga tatagacaac gtaacgccgg aaatcgtcgt ggattatctg acaaagctta
     2221 gagaagaagg tttggcggcg aattccatga acagagcgct ggccgctttg aggggatttt
     2281 acaaatatct gctgagggag aaggttattg cccaaaatcc gctggcccat atcgagctgg
     2341 cgaaagtctg gatgaagctt ccggaaacga tcagcaagga agaaatgaaa accattctgg
     2401 ctcagcccgg aaaccggaat aatgctgcca ttcgcaatac cgccatgctg gaacttttat
     2461 acgcgacggg catccgtgtg tcggaactca ttaatttgac gatgaacagc atcaactggc
     2521 aggtgggatt tctgattgtc atggggaaag gcagtaaaga aagaattgtc ccgatcggca
     2581 gaatagctta tgattgtgtc aggcgttatg tggatgaaat ccggccgaaa ttcatgcaga
     2641 aaaaaagcac ggatgttctg tttttgagcc ggttcggaaa aaaattcacc aggcaggggt
     2701 tttggaagat cgtggttgcc tgtgcgcgtc aggcgggatt gcagaagaag gtttatccgc
     2761 atacgttccg gcattccttt gcgtcgcatc ttctggaagg cggcgccgat atgcgtaccg
     2821 tgcaggttat gctgggacat tcggatatcg caaccacgca aatctatacc cacattacga
     2881 gggaacggtt gaaggaagtt caccgcaaat ttcatccgcg ggcttgaaga acaacaggaa
     2941 agccgttatc aaaagcatcg tcatgcagaa acgagaaatg cgatgatcca tcaggataga
     3001 caggatagaa gaccaaaatt aaatggaagg ttgccggtaa aaacgctatc cattgcagcc
     3061 gccgttgttg tttgtatcgc cgttgtggcg cttctttttt atcagggatt ttttttgtcg
     3121 ctggcttttc agatgtccag gatcaaaaat accttttcgt attatgttct gaaggagaag
     3181 ccgcaattct attatttgga cgtggaaaaa aacggtcagg acatgcgcat ataccggaat
     3241 gacgtgctgg aaattacata ccgcgatgag gttgtcgtca aagcggtggc cagtgatgat
     3301 ttatccggca agtataccta cgtccggttt gaagggctca gcagcaggag cagcgacctc
     3361 ggtgttttga tgaagggtat tgattttgtc aataagctga tcaggacggg aggaatgagc
     3421 ggtcgttccg gtacggtgaa caattatcaa atccttgtgc attatctgaa tgacacgatc
     3481 gcgtccgtgc cgctgaaaat tgtcattaca cctcaagact ggtttaggtt tgcgaaagaa
     3541 tcttccaacg tcgaacagca gattgactat ttaaagaaag ccatcgaact caatcaaaac
     3601 gatgtccgtg tgcgcaagat tctggccggt atttatgcgc atcacggaag aacggatgac
     3661 gcgaaaaaac tgtatcagca ggtcctgacg atccaatccg atgacacaac cgccatgctg
     3721 gagctggtga aaattcatat caaacaaaag gaattcggcg ctgcagcgaa gctcgccggc
     3781 gacctcatta aaatcaatcc caaagaaacg gagggccatc tggcgctggg acttgctctc
     3841 gaggaaaaaa aagcatggtc caaagcggcg caacattacc gggaagccgt ccggctggaa
     3901 ccgcataacg acgcggcgag attcagactg gcggacgcat acaaaaacgg caatatgatg
     3961 agcaacgcca ttgacgaata ccaatacatc gtcaaacatt cccgggagcc ggaggaggcg
     4021 ctgtttgcgc tcggtgatat ttatctgaaa ctcaaaaaaa ccgatgaggc gattaaatgt
     4081 tatcgggaaa ttttgagacg acagcctaaa aatccggtag cccatgcgaa tctggcggtg
     4141 gcatatgccg gaagcggcaa ggcgcaggag gaactgcaaa gtctgaaaaa ggcggccgcg
     4201 cttaatccca acgagcctac catccggttc aatctcggtg ccgtttacga aagaaaaaaa
     4261 atgtatggcg atgcggcgag agaatatcgc catgttctga agatcaatcc cagcgatacc
     4321 gatgccctgg agaggctggc ggatctgtca ctaaaaaaca aacagtacag ccaggcggtc
     4381 aactattatg agaggctgaa aacaaaatca tcgcgcaagg cctcggttta tgcgaatctc
     4441 ggttttgcct atggagaatt aaaaaaatac caggctgcgg cttcgaatta cgaaaaggcc
     4501 attcaactgg gcgcgaagga accggttttg cgtgccaatc tcgcggatac ctacgaaaag
     4561 atgggcatga aaaagaaggc gattgccgtg tacgagaaga tgcctgcgtc gtcaaaagac
     4621 gcgtcgaccg cgctggcaga tctttacctg aaggaaaaaa attatcagaa ggcgatcgca
     4681 atctatcaga agctggccaa acaggaacct aaaaaggcgg gatcttacgc caatctcggg
     4741 ttcgcctatg cggctgccgg taattacgat caggccatca aacattataa tacggccttg
     4801 aaattcgata gcgacgatgc cgatatttat gataatctcg gagaagcgta tgagaaaaaa
     4861 gggctatatc agaaagccct ggaaacctat aaaaaagctc atgcactcaa tccggaatcg
     4921 ccaaaagccg ctcaacggat cccccgattg aatattcaat tattacagga gagaaagcgg
     4981 caaaatacag ggagtgaata aaaatgaaaa aaataaaaat gggtaatgtc acaatcggag
     5041 ataataaaaa atttatattg attgccggac cctgcgttct ggaagatgag aaaaaggcga
     5101 tggcgattgc gctttatttg aaaaaactca cggagaggct gaaaatgcct tttatcttca
     5161 aagcgtctta cgacaaggcc aaccgcacgt cgattaaatc cttccggggc cccggattga
     5221 aaaaaggtct ggcgatattg cagcgcatca gggaaaaact gggtgtgccg gtactgtccg
     5281 acgttcatcg tttcgaagaa atcgatccgg cctcgaaagt tctggacgtc atgcaggtgc
     5341 cggcattttt atgccgtcaa acggattttg tcaccgaaat ggcaaaaaag gcgcgtgtga
     5401 tcaatgtcaa aaaaggacag tttctggcac cgtgggatgt ggccaatatc atcgaaaagg
     5461 ctcgtgcggc aggcaatgac aacatcatga ttacggaaag gggcgcaacg ttcggttata
     5521 ataatcttgt ttttgatgtg cgttccctgc cgatcatgcg taagatgggg tatccggtca
     5581 tttttgatgc tacgcattcg gtgcagttgc ccggaggccg gggggcgtct tcgggaggac
     5641 agagagacat ggtgacgtat cttgcgcgcg ctgccgtggc tgccggtgtt gatggtgtct
     5701 tttttgaagt ctatcccgat cccgagaaag cgctgtgcga cggtgcaaat tcactctatc
     5761 tggattcgct ggaagacctt cttgtcactc tgatgaaaat cgacgcttta gtcaaaacgc
     5821 cgtcaaacgg gaaaaatcaa taaaagaaaa aaggctgaat aaaaaatgaa aaagaatctc
     5881 aaggaaaaac tcaaaaaaat aaagctgctg atgctggatg tggatggcat catgacggat
     5941 gggcggatta ttatgaatga tgaagggtgt gaaacgaaac atttcaatgt ccgcgatggc
     6001 catggattga aaatcctgca gcgctttaat atcaaagtgg ctattattac cggtcgtcaa
     6061 tccaaagttg tctcgcatcg ggcaagagat ctggacatta cagatgtcta tcagaaagta
     6121 tacaacaaga aagaagtctt tgagaaaatt ctaaaaaaat acaagctctc gccagacgaa
     6181 acggctttta tgggggacga cattgtggat atcccggtgt tgaacagggt cggtttttca
     6241 gcgaccgttg ccgacgcatc cgacgtggtc aaaaagcagg ttgattatat aaccggacat
     6301 aaaggcggaa agggcgccgt acgggaagtc tgtgagatga ttctgcaggc tcagggaaag
     6361 tgggcggaga ttgccgcgcg atatgaattt ttaaaataac atttcgtcat ggcttttttt
     6421 aaacggtttt tataacgcag tacacccctt atagctttac accctgcatt ctgagtgata
     6481 taataaaaac aaataaaaaa agtttctgcg ggatgtttcg gattgtgata aaaaacagaa
     6541 aaaaaacaat agttttcttc tccgttgtaa cggtttttct ggcggttttt ctggcagctt
     6601 ttttaatctt cagggatagc atcaataagc cgaaaacgtt attgaaggcg ctgcaggata
     6661 acgttgatct gcaaattaaa gggtttgtct atacggaagt tggagagaat aagggaaaat
     6721 gggaggtaaa agcggcatcg gcgtcatacg ataaaaaaca aaatcttgct gttttggagc
     6781 aggtccgggt aacggtgata acggcggatg gtaaaaagta tgaaatgaag gcggataagg
     6841 gccggatttt tacggataag aagaacatga acatcagcgg caatgtcgtg atcacgtcgg
     6901 atgacggcga taattttttc acggagtatg tccaatacag cgatgaggaa agaaaatttt
     6961 tcacggacgc gccggtcaga atggaaagca gacgcatcag aataaccggg cagggattgg
     7021 ccattttttt gaacagcggg gaattgagta ttccgtcgat ggtcaaggca aacattcatt
     7081 gagaaaagaa tgatccgtaa acacacatac atttttttaa tgctgttttt tttcttttgc
     7141 tacgttgaaa tctgcccggg taaaaataca ttgaccgccc gcacggacaa gccgattcag
     7201 attacagccg caagaatgga ggcgtttaag aatcggagcc agatcgtttt ttcgggaaat
     7261 gccacggtcg ttcagggcga cagtgtatta aaggcggaaa aacttatttt gcattatcgg
     7321 caacagccga aaacagagaa ggcgcaaaag gcgcgcgaca atcaagccgg ggatctggaa
     7381 aaaatcgagg cccggggaaa tgtttccgtg gtgcagggaa cgcggaaggc aacggccgat
     7441 gaggccgtat atgttcaggc ggaaaataaa attatcatga cggggaatgc ggaactgaag
     7501 gaaggaaaaa gttcgatcag aggcgagcgg gtgattattc tgcttgatga agataaaggt
     7561 attgtggaag gcggagagcg catgcgcgtc aaggcggtta tctatccgca ggagcgcaga
     7621 cagacagaaa accagtcaac catgaaaaac tgatgattta aaatgagcga tttatcggct
     7681 aaaggtttaa cgaaaattta taacaagaga aaagtggtca atcagatcga cttattgatt
     7741 gcaccgggag aagtggtcgg tctgctgggg cccaatggcg ccggaaaaac gacgacgttt
     7801 tacatgattg tgggattggt caagccggat gcgggagaga tttatctcga cggggaaaac
     7861 attacgggaa atcccatgta ccggcgagca cgcaaaggcc tgaattatct gcctcaagaa
     7921 ccgtcgattt tccggaagct gactgtggag gaaaacatcc tggcgattct ggaaactctt
     7981 gatttgagtg aagcggaaag acaggagcgg cttcgcgagc ttttaaagga gctggacctg
     8041 accgcactgg caaaaaatta tgcctattct ctttccggtg gagaaagacg gcgggtcgaa
     8101 attacacggg ctctggtgac ctctcccaaa tatattcttc tggatgagcc gtttgcgggc
     8161 attgatccgc tggccgttgc cgatattcag aaaattattg aaaaactgaa agccaagggc
     8221 atcggcatca ttatctccga tcataatgtg cgggaaacgc tttcctgctg tgaccgggcg
     8281 tatatcgtca acaatggaga gatcctggtg gaaggaccgc cggacaccat tgcgcagagc
     8341 gaactggccc gaaaatttta ttttggagat tcatttaatt tataacaggc ttgtactgaa
     8401 ttccgattat ggcatttgag ttaaaacagc atctaaaact ttcccagcag ctgatcatga
     8461 cccctcagct ccagcaggcc atcaagctgc tgcagctgtc ccgtcaggaa cttgtggaag
     8521 cgatcaatca ggagatggaa gaaaatcccc tgctggagga aatttccact gatgaaaatt
     8581 cggacgaggc attgatcggg gaatttgaaa atgatggcct gcccgtcgaa cgcgatacga
     8641 taaaagcggt cgaacatacg gaagaattga atgttgaaaa aggagccgga acggacgaat
     8701 tcgactgggc gagctacctg gaagattacg gcccggtggg catgacgtac ggcggcaagg
     8761 atggggagga aacatcctgg gataatgtgc tgacagaagg tcagacgtta tcaaagcatc
     8821 tgacctggca gatgaaactg tcgtcctttt cggaagacga agaacgggtc ggcagccaga
     8881 ttatcggcaa tctggatccc aatggatatt tatgcgcaac ggctcaggaa attgcacaac
     8941 tggaaaatgt cagtgaagag ctggtggaaa gagtgcttca aaaagttcag gaatttgatc
     9001 ctccgggaat tgccgcgcgt aatcttcagg aatgcctgtt gatacaggcc aggatgctgg
     9061 gcgtaaaaaa ccggattatt gaagtgataa tcagagattt tctcaaagag ctggaactga
     9121 aaaactatgc tcaaatcgcc cataaattaa aagttccgct caaggaagtg gaaatggcgg
     9181 tattgctgat cagcgaaatg aatccgaagc cggggagcat ttattcggaa gacaaagcgc
     9241 agccgatcat tcccgatgtc tatgttgtca aaacaggaga cgaatataaa attattttga
     9301 acgatgatgg cttgccgcga ctgcgcatca gtaattttta ccgggaagta atggccggca
     9361 tttcgagtca tagtcatgaa gaggccgaaa acgggaaaaa atacatcaag gacaaggtac
     9421 aatcggcaac ctggttgatc aagagcattc aacagcgtca aaacaccata tataaggtgg
     9481 cggaaagtat tgtaaaattt caaaaagaat ttttcgataa aggcatcgat tatctcagac
     9541 ctttgatttt acgggatgtt gccaatgata tcgagatgca tgaatccacc atcagccgcg
     9601 tggttaataa taaatacatg cattcgccgc agggaatttt cgaattgaag tatttcttcg
     9661 gaagttcgat cagaacgaca acacagggaa cgattgcatc gaaaagcgtg caggaagaaa
     9721 ttcggcaact gatcagctcg gaaccgccgc gtaaacccta cagcgactgc gaaatggttc
     9781 aattactgaa ggcaaaggga attcatatcg cccgccgaac ggtggccaag taccgggaaa
     9841 tgatggggat tcttccttca tcgaagcgaa agaaatattt taaagcgatt tgacggacaa
     9901 agacacaacc ggtgtaagat tttggttgac ttttagtttt gagcccaata tatgacgggt
     9961 ccctttatgg tgaaactctg gtgccgaagc gaagcatcgg gattggcagg ttgaaaaaca
    10021 cggcgcagcc gaggcttcaa ttctatgcag cggtttggca aagaggttcg cacctttgat
    10081 tgccggcgca aagaaagtca atcggtcgat gaaggttatc aggacgatga atttaattat
    10141 gtaaggagga gcaatgatga agatttcggt aacgttcagg aacggggaag gggagaactg
    10201 gcaaaaaaat tacgccgaag aaaaaatgca gaaactgaaa aaatatctgg atgcgcccgc
    10261 cgaagctcat gtagttgtct ctatggaaaa gttcagaaat gtgacggaaa ttaatttgaa
    10321 ctccaacgga tggaacatca acgcgaaaga agaagccaag gatatgcgcg ttgctctgga
    10381 ccggtgtgtt gaaaaaatcg aaaaacagct caagaaacag agagagaaaa taagacaaca
    10441 caaaccgaag acgatcagac gtgctcagga aaaacctctc aacgaggaag aaatcgagga
    10501 accgacggca agtaaaatca tggaaacccg aaaagttatc ttaaagccca tgtcgtttga
    10561 tgaagccatc atggaaatcg aggaaacaaa tgaccgtttt gtgctgtatc gcgatacgtc
    10621 gtctgaaaat gtcagtatga tctacagagg ggatgacggc cgttacactc ttatcgaaac
    10681 gaaatgaaag cgtcggagct tttctagagg tgatcatcaa gcaagtcaat agacatggaa
    10741 gatatggttg aacaatgcag ctgaatcaga tttttaaaat ggaatttatt aatgccggtt
    10801 tagaggcgaa aaacaaggat gaagtccttg cggaactcgt gaatgttatc attgaaggcg
    10861 gtttaaaact gaacagttct tcaatcattg atattttaaa gcagcgtgaa aatctcggtt
    10921 ccacgggcat aggagagggg gttgccattc ctcacggaaa gatatccaat ctgaatgaca
    10981 ttgttgttgc tttcggacgc agcgaaaagg gcattgctta tgattcgctc gacggcaaac
    11041 cggtgcatct cttttttctt ttgcttgcgc cggaaaactc tgccggccag cacctgaaaa
    11101 ttctggccaa aatatccaaa atgctcaaag acgtttactt ccgtaacaaa ctcatcgcgg
    11161 caaaatcccg ggatgaattg tatcagatta tcatcgatca ggaacgcacc tgtattgtct
    11221 gaacgggaca agacatcaaa acgttttttc ttttgccgca ggaatccgtt gggggcctgc
    11281 ggctttttat tgttgtaaaa tttataaaat gtgtgctaaa aattcggcac atccagggga
    11341 aataaaatga aaaatttgcg ggttgttatt ataacgggca tttccgggtc gggtaaaagt
    11401 acggcattgc gcgcgctgga ggatgtcgga ttcttctgcg tcgacaacct tcccgttatt
    11461 ttactgccga aatttcttgc catcacgctg gcttcttcac cggaaatcaa gcgcgtggcg
    11521 ctggttatgg atttaaggca acgaagcttt ctggataaat acagccggat ttttgcgagc
    11581 ctgaagaaaa aaggatacaa aatcgagatt ctttttctcg agtccagcga cgagtctctt
    11641 gtccaccgct tcagcgagac cagacgtgtc caccctctcc tggaacgggg cattattatg
    11701 gaaggaattt cgctggaacg cgaaaagtta tcttccctga agaaaatggc ggataaggtg
    11761 atcgatacaa cgtcctttaa tgtgcatcaa ttaaaggacg ttgttcagag gtctttttcg
    11821 gtattttcaa aaaacaagag aatggtaatc aatgtccagt cgttcggtta ccgttatgga
    11881 ttgccggtgg atgccgacct tgttttcgat gtccgatttt tacccaaccc ttattttata
    11941 gaaaagttga aaaattttga cggtaataac aggaacgtgc aaaactacgt cttgcgcaac
    12001 agggaaagca aacagtttct ggaaaaaatc aaagatttaa tgaatctctt gaccccgctt
    12061 tatgaaaaag aagggaaggt gctcctcaat attgccatcg gttgtacggg cgggaagcat
    12121 cgatcagttg tgatggccaa taaaatgagc gcttttttta cctcgctgaa gtaccatgtc
    12181 aatgtcaatc accgggatgt caataaacat taaaagccga tggccggttg aaacgctgct
    12241 ttaccgggtg atgttgtctt tgatccagtg gggatcccac cattctttgg ggtcgacaaa
    12301 ttttccgccg accataatac tgaagtgcag atgatctccc cccgccagtc cggacatgcc
    12361 ggaaagacca attttttcgc cttttgccac ggttttgccc acggtcgtgt cgatggatga
    12421 gagatgggcg taaagactga ataatcccag accgtggtcg ataataaccg tattgccgta
    12481 aatgcccaga gctccggtga agacaacaat ccctttattg gatgcctcaa tggggctgcg
    12541 cgcgttggca gcaagatcca cgccttcatg aacggattca cccaccactt ttccttcata
    12601 cgcataactg cgcttatcgc cgaacagggc cataggagcg gcattgctca tgcgcaggaa
    12661 ttcgccttcc cagagttctt tgggagccga tttcatgcag attttctgta tggcggaaaa
    12721 gttttctttt cgcatctggg aattgacata ggtgaataca tccagaagcg ttttgccctg
    12781 aagcgaagga accatggcct ggaattccgg cattttacgc tggaggaacg cttccgaaag
    12841 atgcattttg tcggaacgga atttcttttc cttaatcacg cagggaacgg acacacgggt
    12901 ttcattgccg gcttcgtccc gggcaaagat cataatgctt gtagctgctt tatcggcagt
    12961 catcggcagc gcgaaatacg tgatgtacga aggtttattg tcgatcaaga cggcatgcgc
    13021 cggcgacaag taatcattga tataaacgcc cgtcgatgag aggggttttg aaggctgata
    13081 cgcgacaaag cccgtgcctc cctgattgat gtaattgata ttctttagaa ggttgatcat
    13141 cggtggaatc gtatcgattg tgacggactg cgacagcacc gtctgattct tcaaaagcga
    13201 atgatccgtc gccgatattt tcaaaaccgc ggggccgtca tggagcgcca gttgagtgct
    13261 ggcgatattc agactcaggg attcatgggc cacgcccttt tgcgggattt ttttatcggc
    13321 cagaacgtga cctttgttgt cctgaatgat ctccgccgtg atccgggaaa gaccgctttt
    13381 ggaatcggaa aacgtaatac cgacagtttt ctgtctgccg atggctttca catcactgtc
    13441 taatttaatc acaggatttc ctatttcgat ataattaccg aagataaacc agcccagggc
    13501 ggcgacgacc acgagcgcga tgagaattct gatatcaaaa cggaaccatt tcataataat
    13561 ctcctaacgt tgtgatgtaa atagaattgc tcggtttata atagcattcc ggaggatttg
    13621 caagggaaat taatgccacg tgctaagagc gaatgtaatg aagctcgaag cgtaagtgga
    13681 attatccccg cagcgaagcg gagcggcgca agcacagtga agaattaagc tacatgggac
    13741 catttcctta taaaattttc aagatatcaa taataattat accttgatat tgtgaaaaag
    13801 cattgttata ttcatagaca acggccaagc gccacgagct gttttcaaaa aaattaatca
    13861 aaaatttctg gaagtcgaac catatgctgg aaaataagaa aattgttctt ggaatcaccg
    13921 gaggcattgc ggcttacaaa gcggcggaat tgacccgccg gcttgtgaaa gcaggtgccc
    13981 atgtaaaaat catcatgaca aaaagcgctc tggaattcat tacgcctctt accatgcaga
    14041 cgctttcgca aaacagagtt tacacggata tgtttgcctc tgatgacgaa tatgacatgg
    14101 cacatatcgc gctggccgaa tttgccgacg cgtttgtggt tgctccggca acggcaaaca
    14161 ttatcggaaa aattgcttcc ggcatcggcg acgatttgct ttccaccacg atcatggccg
    14221 agaacaaacc gacgctgatt tgtccggcga tgaacgacaa aatgctggcc aatccgattg
    14281 ttcaggacaa tatccggaga ctgaaaaaaa acggttacgc agtcatggac agcgccgtag
    14341 gcgaactggc ctgtaaaaca gagggcaagg gacgcttgcc ggagattgac gcgatccttg
    14401 ctgaaatcga aaacattatg acgccgcagg acttggcggg cgtcagaatt ctgatcactg
    14461 ccggtcctac agaagagccg ctcgacccgg tgcgtttcat cacgaatttg tcgtcgggaa
    14521 aaatgggtta cgctctggcc tccgctgcgc aaagacgggg agcccgcgtg acgctggtca
    14581 gcggccccac gtcactgccg ctgccgccgt tggaaaaaat cgtcacggtg cgtacggcca
    14641 gagaaatgca caaagctgtt ctgcagcatt ataaaagtgc aagggtgatt atcaaggccg
    14701 ccgccgtagc cgattatcgt ccgaaaatcg tggccaatga aaaaataaaa aaggaaggga
    14761 aaacgctatt tatagaactg gaaagaaatc cagatatcat cgcggaaatc ggcaaaaaaa
    14821 agggaaaccg gattctggtc ggttttgcca tggaaacgca gaatctttta accaatgccc
    14881 ggggaaaact ggtcaagaaa aacatggaca tgatcgttgc caataatctg cgtcaggagg
    14941 gtgccggttt ccgcaccgac accaacatta ttaccgtcat cgaccgcgcc ggtaagacgg
    15001 agtcgctgga gaaaatgaca aaaatcgatg cggccggtgc gattctgaat cgtattaaaa
    15061 aattgatttc tgaaaaaagc aaaggcaaga aataacaggc gaacgatgtc gaacataacc
    15121 agaaacgaat tacaagccga attactccat gcggtcaggg ggctgaaagg acaggtcatc
    15181 cataacgaga gtctggcggt tggcgtggat tggagcagat catcacggac cgttgttgct
    15241 aaagccaaga ccggagggca atccagaggg gaaatgctgg aagccataca ccaagagatg
    15301 gccgattgtc agctctgtcc gctgggcaga acccggaaaa atcttgtttt tggaacaggc
    15361 aatcccggag cgcggatcgt ttttgtcggt gaagctcccg gcgctgatga agatgagcag
    15421 ggcatccctt ttgtgggcag agcaggccag cttttgaccg aaattattgt taaaggcatg
    15481 aagcttcaga gaaaggatgt ttatatctgc aatatcctca aatgccgtcc tccgggaaac
    15541 cgcaacccgc agccggatga gatcagcagg tgcgaacctt ttctcaagaa acaactccag
    15601 ttgatagcac cggaagtgat ttgtgcattg gggacgtttg ccgccaagac gctgctaaaa
    15661 acagatgttc ccatttcagt gctgcgtgga aggtttcatt cctatgaagg aataaaatta
    15721 atgccgacct atcatccggc ctatcttctg cgcaatccgg cggcgaagaa gcaggtatgg
    15781 gaagatgtaa aaatcatcat gaaggaatta aaccttccgt attgagccat caaacgaggt
    15841 aaggcaatga agcaaatccg atatgcatta atggctgttt tcattttcgc aggacttctg
    15901 gcgtgggcag aggaaaaaaa tcctattttc aatgttatca cggtggatgg tgtcattacg
    15961 tcaccgaccg cgaaatacat tgcatcaggc atcgaggaag ccgttcggga taatgccggg
    16021 gggctcatca ttctgctgga tacgccgggg ggcatggata cggcgatgcg cgatatcgcc
    16081 aagagcattc tgaattcacc ggtacccgtg atcgtttatg tatcgccgtc cggagcgcgg
    16141 gccgcttctg cgggcgtcat catcacccag gcggctcaca ttgcagcgat ggcgccggga
    16201 accaatatcg gcgccgcgca tcctgtggca atcggtctcg ggaattcggg cggagaaaaa
    16261 gataaaacca tgacggaaaa aattgaaaat gatgccgccg cctatgccag aagcattgcc
    16321 aaaacaagag gccggtcgga agaatgggtc gaaaaagcgg tgcgtaaaag cgaatcgatc
    16381 acggcggagg aagccttgaa gctgaatgtc atcgattatg ttgcggcgga tgttaacatg
    16441 ttactggctg ccatccacca gaaagagatt cagttgatcg gcggcaaaaa aataatatca
    16501 acaaaaaacg cggtgattcg cgataaaaaa atggggacgc gtcagggtat tctttccgcc
    16561 atttcggatc ccaatatatc atatcttcta ttactgatcg gactggcggg gctttacttc
    16621 gaactgtcca ctcccggcgc gattctgccg ggcgtgatcg gcggcatttc gcttttgctt
    16681 gcgtttttcg gcttgtccac gctgccggta agttatacgg gaattttgct gataatattc
    16741 ggcgtcattt tatttatcgc ggaaattaaa gtcatgagtc acggcatgct gacggtagga
    16801 gggattattt cattggttat gggatcgtta ttgctgttcg atacgccgga gccggccttg
    16861 cgtctttcct tgcaggtatt gataccggcc gttgtggttg tctctatttt ctttatcgtc
    16921 atcattatgc tggcagtgaa ggcgcagatg agaaaacatt ttaccggcgc ggaagccatg
    16981 attgatctgg aagccgaagt aaccagcgac atcgaaaaag agggcgatgt ttttgtcaaa
    17041 ggagaatact ggaaagccgt cagtgacgcg tccatcaaaa aaggcgccaa ggtgagaatc
    17101 gtcaaggttg acggcatgaa actggtcgtt gaagaaataa aaaatcaaaa ataaggatta
    17161 gaataaaaag aggaggaata catatgttgg gaactttatc tatgccgacg ctggtcgtga
    17221 tcgttctggt cgtaatgttt ttggtctccg caatcaaaat attgagcgaa tacgaaaggg
    17281 cggttatttt ccgactcggt cgaatccggg atgccaaggg gccgggcttg attatcatta
    17341 ttcccggcat tgacaaaatg gtccgggtgg atatgcggac cattaccatg gatgttcctc
    17401 cgcaggacgt tattacgaag gacaacgttt ccattaaagt cagtgcggtt gtttatttcc
    17461 gggtgatgga tgccaattcc gccgtgacga atgtggaaaa ttatctttac gccacatcgc
    17521 agcttgctca aaccaccttg agaagtgtct gcggccagat ggaactcgat gaaatacttt
    17581 ccgaacggga aaaaattaat atgaatcttc aggaaattct cgaccgcagc actgaaccct
    17641 ggggcattaa ggtgtcgctg gtagaagtca aacatattga cctcccggaa gagatgaaac
    17701 ggtctatggc aaagcaggcg gaagcggaaa gagaacgaag agccaaggtc atcaatgccg
    17761 aaggcgaatt tcaagcagcg cagaagctga tcgaagcggc tgctttgatg gaaacccagc
    17821 caatgtcctt acagcttcgc tatttgcaga cattgaatca gatagcttcg gaaaataatt
    17881 ccacaacggt atttccgata ccgattgatt tgtttaaacc ttttttgaaa gataaataat
    17941 ttccggaaaa aagcagagac gctttaaata accaatacac gaaaaggaga ttcttgcatg
    18001 agcgataaga aacagaatgt gattacgggc ggaacggttt taattacgtt gggaattttg
    18061 atttatctga gcaagtccgg aagtttcccg ttcggccaga cctggccggt tttgttgatt
    18121 gtcatcggta tctgcacgct aatacaaaag ttcagagatc tcggcggatg gattataacg
    18181 cttgccggga cggggtttct cttttatgag ttctacggat tcgatgtttc ccagtattc
//
LOCUS       DAOOLB010000038        17579 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_3351_length_17579_cov_50.428384, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000038 DAOOLB010000000
VERSION     DAOOLB010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 17579)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17579)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17579
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3351_length_17579_cov_50.428384"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PLF77_06325"
     CDS             complement(<1..208)
                     /locus_tag="PLF77_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08504.1"
                     /translation="MPDFEQLSLSFRNVVKRLPASPFIGFLQNSGRKPVKVTQTALII
                     TFIIEDIKHGYYTGQIARYRTALPG"
     gene            complement(329..562)
                     /gene="rpmE"
                     /locus_tag="PLF77_06330"
     CDS             complement(329..562)
                     /gene="rpmE"
                     /locus_tag="PLF77_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HPC08505.1"
                     /translation="MKEGIHPDYQETTITCVCGNVIHTRSTKKDIKTEICSNCHPFMT
                     GKQKIVDTAGRVERFKKKYETKVDATTSKKKKS"
     gene            complement(699..1946)
                     /gene="rho"
                     /locus_tag="PLF77_06335"
     CDS             complement(699..1946)
                     /gene="rho"
                     /locus_tag="PLF77_06335"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPC08506.1"
                     /translation="MNIEDIKRLPISELNELGKKLDVPGASGMRRQELIFAILQTQAE
                     QNGVISGSGVLEILPDGFGFLRAIDYNYLPSPDDIYISPSQIRRFNLKTGDTVSGEVR
                     PPKEGEKYFALLKVDTVNFESPEQARDKILFDNLTPLYPQEKLNLEYDPKNYSTRTLD
                     LFAPIGKGQRGLIVSPPRTGKTVLLQDIAHSIAANHPEVVLMVLLIDERPEEVTDMQR
                     SVSGEVISSTFDEPATCHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVVP
                     PSGKVLSGGVDSNALHKPKRFFGAARNIENGGSLTIISTALIDTGSRMDEVIFEEFKG
                     TGNMELHLDRRISDRRIFPAFDLIRSGTRKEELLIPKNNLNRVWILRRLLQEMNPVDA
                     MEFIVGKIKKTENNQEFLDSMNS"
     gene            complement(2238..2408)
                     /locus_tag="PLF77_06340"
     CDS             complement(2238..2408)
                     /locus_tag="PLF77_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HPC08507.1"
                     /translation="MALNKKLLEILICPKCRGKVRFDEKANGIICDKCKLLYEIKDDI
                     PVMLVEEAKNID"
     gene            complement(2395..3459)
                     /gene="waaF"
                     /locus_tag="PLF77_06345"
     CDS             complement(2395..3459)
                     /gene="waaF"
                     /locus_tag="PLF77_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HPC08508.1"
                     /translation="MDIEGQNVNILIVKLSAIGDVIHTLPSLAELRRLYPDAHITWVV
                     EEAAADLVKHHPCLDEVLISRRKTWIQNVRAGKIRQSLNEGISFIKGLRKRHYDLVID
                     FHGLFKSAIIVFLSRGRRKLGYDSWQELSGLFLNEKIPEDMDKHAVDRYLDFLRHLGA
                     KTPEARFVLPSNDEEEKKAQLLMQKHRLEDKKFIAVNPVALWDTKLWSNEKFARLADL
                     IQNRLKLNVVFTGSDKKPLEAITALMTTAGLHLGGETTLICLAGIYKKARAVVTTDSG
                     PMHLAAAVGTPVIAVFGPTDPARTGPYGSRHTVIRSGEDCSPCFLKTCPEKKCMDHIT
                     PEQVFAAVEKTVMEEKNGVK"
     gene            complement(3435..4022)
                     /locus_tag="PLF77_06350"
     CDS             complement(3435..4022)
                     /locus_tag="PLF77_06350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPC08509.1"
                     /translation="MKKNTAVFLDRDGVINEEVGYLDGLEKLKVIPNACKAIQMINQS
                     GMKAIVISNQSGVARGLFTEEFVQTANSHLQKILKTHGASIDDFYYCPHHPSEGKAPY
                     RQICDCRKPAPGMLLRAARDLNINLTLSYLVGDRYIDMIAARRAGVKGILVKTGYGND
                     LLQDDGPDRPTPESEPDFIAADILEAVQWILKDKT"
     gene            complement(4019..5086)
                     /gene="waaF"
                     /locus_tag="PLF77_06355"
     CDS             complement(4019..5086)
                     /gene="waaF"
                     /locus_tag="PLF77_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HPC08510.1"
                     /translation="MKDEKIKKLPQKGIHKILIRGTNWIGDAILTLPAVASIRATYPQ
                     ARIAMLVKPWVADVYDIFSNLDEVIVYENKYDTATGVFGLARMLREKQFDAAILLQNA
                     IEAAIIALFARIPVRAGYNSDARGLLLTHSVPLTRDIKKVHQVNYYLEMVKALGCVSA
                     NRDMNLETKISREEAQNVLRRYVAPSQKMLIGIAPGATYGAAKKWFPDRFAETLNRLD
                     EQFSVQGIILGGKADRITAEEVRTSARADMINLAGETSLREAIYLISQCRLFISNDSG
                     LMHVAGALNVPTIAIFGSTNPTTTSPVGKQSLIVRKEVSCSPCLKKTCPTDFRCMNLI
                     TVEDVLSVAQTLLKDNRDKDQ"
     gene            complement(5097..6209)
                     /gene="lpxK"
                     /locus_tag="PLF77_06360"
     CDS             complement(5097..6209)
                     /gene="lpxK"
                     /locus_tag="PLF77_06360"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPC08511.1"
                     /translation="MKTITDWPRIWDNDGSASGYGTIKFIAHLLSFFYLSIVNLRNWL
                     YDHNLLKATRLGCPVISVGNITVGGTGKTPCVVMLARMLQHRGFKPAVISRGYGGRGK
                     GAVNIVSDGQRILLDSEIAGDEPLLMAHALTGIPVITGPRRTATGKTAIDHFGADVLI
                     CDDAMQHRQIFRDVNLVLIDGQDLKDNIHVLPRGRLREPVKALRRASAVVFTRVDGQI
                     AVHQTIENLITKENLPVFQSLHRINDMISADKKICKPFTELEGKRVHAFCGIANPESF
                     KKTLLSTKAVLVAFNIFPDHHRFQEHELEKIKNDFKNSAADYLITTEKDAMRLKNHPE
                     MSKMLFVLRITMEIKDNPQSFENFILHKIRAGTKKG"
     gene            complement(6172..7935)
                     /gene="msbA"
                     /locus_tag="PLF77_06365"
     CDS             complement(6172..7935)
                     /gene="msbA"
                     /locus_tag="PLF77_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HPC08512.1"
                     /translation="MKIFKRLLSLGRPYSVRFLISMLCMVIAGALTSSQAVLVKPVLD
                     DIFLAKNPASLKWLPLAVVMIFLVKGICNYVQTVLMNFIGLRIVTDLRNQLYEQIQKQ
                     SLSFFTHHPTGLLMSRITNDVASIQNAVSDVVTSIVKDSFTIVALICVIFYTDWKLAF
                     IAMIIFPLTIYPISAFGKKIRKVTTSTQITMANLSSLLQETIAGTRIVKAFGMETHEI
                     KRFAGENERFFKLNMKAVSVKALSHPLMEFLGGLGIAAVIFYGGYNVLAGNSTPGTFF
                     SFLTAVLMLYEPVKRLTNVNNTINQGIAGAERVFSIIDLVPDIQDKPHAAELPPIDRG
                     IEIDNVTFRYEKTPVLKNITLSIRAGEVVAFVGMSGGGKTSLVNLIPRFYDVSEGRVL
                     IDGLDIRDVSLPSLRRQIAIVTQQTILFNDTVRNNIAYGSFEKSDDEIICAAKAANAH
                     DFIMRMPKGYDTNIGELGTKISGGEKQRLSIARAILKNAPILILDEATSSLDTEAEME
                     VQEALDNLMKGKTTLIIAHRLSTIRNADRIIVLVNGEIVEEGNHETLMQKQGEYFRLY
                     NLQFKDEGSHENNNRLAKDLG"
     gene            complement(7952..9100)
                     /gene="lpxB"
                     /locus_tag="PLF77_06370"
     CDS             complement(7952..9100)
                     /gene="lpxB"
                     /locus_tag="PLF77_06370"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPC08513.1"
                     /translation="MKDSNHKNKTVLIIAGEASGDMHGANLVREMLNRDPALKFYGIG
                     GKKMLQEGVQLLADASTMAVVGLTEVVFKLKDILKIMRTIKKSLDERRPDLVVLIDYP
                     DFNLPVAKAARRHNIKVLYYISPQVWAWRQSRIEQIKKTVTRMAVILPFEVETYRRHG
                     FAADYVGHPLLDMIKTTYSKKESRKKFDLHEDKITIGILPGSRMSEIKKLLPELLQAA
                     TIMKDSIPELQFVVPLADTLEEKNLTGIIAGFPIDVNVISGQTYDVVSCADLALVASG
                     TATLETALLKVPMIIVYKISPLSYFIGKLFVRVKNIGLVNIIAEKTIVPELIQNDASG
                     ARIAREALSILGDETKKQAMIRELEAIRSRLGEPGAAIKTAKLALDML"
     gene            complement(9121..10215)
                     /locus_tag="PLF77_06375"
     CDS             complement(9121..10215)
                     /locus_tag="PLF77_06375"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPC08514.1"
                     /translation="MIPMVDLKRQYHSLKRDIDAAISNVLEDTRFILGPNVSALEEEV
                     AAYHGPSQAVGVANGTDALLLALRASGIEAGDEVITTPFTFIATAEVVALLKARPVFV
                     DIEPVTFNMDWRQIEGKITSRTKAIIPVHLFGHPADMDPIMAIAKKHNLRVIEDCAQA
                     FGAVYHGRKVGAIGDIGCFSFFPSKNLAGYGDGGMIVTRSDETAAKIKMLRNHGSARR
                     YYHDEVGYNSRLDEIQAAIIRIKLKKIDEFNAARRRNAALYCAAIKRKDIVLPSASGD
                     CEHVYHQFTIRSDERDLLADALQKKEVASAVYYPVPLHRQNAFLNLYDGPETMPQSET
                     CAREVLSLPMFPELDQKEIEFIADIINKAS"
     gene            complement(10212..11165)
                     /locus_tag="PLF77_06380"
     CDS             complement(10212..11165)
                     /locus_tag="PLF77_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPC08515.1"
                     /translation="MKHKKIKVGVIGIGHLGNYHLQKYRKLEPCEIVAVADTSSERAQ
                     KAAQIYQCAAFSDYREMIGSVDAVSIAVPTSDHYNVARDFLAAGVDVLIEKPLCATLA
                     EADDLIGLARKKKLILQVGFVERFNPAIMALENVIRKPLFIESHRLHPFFERGTDVDI
                     ILDLMIHDLDILVKFVDAPAISVEAMGVPILSDKIDIANARITFSTGCTANITASRIS
                     AKTMQKIRFFSPEGYHSVDCQKREVLSFSKGTTGSGQMQIVQNHLEIGSHDPLEEEIR
                     SFVDAVITRGCPPVSGEEARKSLALAVDIITKMKAAEDLLT"
     gene            complement(11176..11949)
                     /gene="lpxA"
                     /locus_tag="PLF77_06385"
     CDS             complement(11176..11949)
                     /gene="lpxA"
                     /locus_tag="PLF77_06385"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPC08516.1"
                     /translation="MKIHETAIIAPGAQLEEGVEIGPYSIIGADVRIGKNTVIGPHTV
                     IDDYTQIGESCRIFQFCSIGAPPQDLKFSGEKTRVVIGNFNTIREFVTIHRSTTADIG
                     VTIMGNHNLIMAYCHVAHNCKLGNHVIMSNAATLAGHVHVEDYAIISGLTGVHQFCRV
                     GAHCMIGGCSAAVKDIPPYTIAQGNHAKLYGLNLIGLKRRNFPEKTIKAISDAYRIVF
                     RSKLLLEDAIKKAESEVEDLPEVRHFIKFIKESERGICR"
     gene            complement(11973..12989)
                     /gene="lpxD"
                     /locus_tag="PLF77_06390"
     CDS             complement(11973..12989)
                     /gene="lpxD"
                     /locus_tag="PLF77_06390"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPC08517.1"
                     /translation="MTTGEIAAFLGGTVMGADDVVIGHIRAIDEAGPGDLTFIANKKY
                     IKKLKTTKASAILVSPQTTAEGKNLIVVSDPYVALGKILSHFYPAQHGRSGVSAEACI
                     EDGADVSPDAVIFPRAFISAGAKIGAGTVIYPGVFIGRNASIGEHSIIHANVTVYHSC
                     IIGNRVILHSGAVIGADGFGFAAPGRDNVKIPQVGFVQIDDDVEVGANTTIDRATLGR
                     TWIQRNVKIDNLVQIAHNVVIGENSVITAQVGISGSTRLGKGVMVGGQTGMVGHITIG
                     DHVMIAAGAGIHKDIKSGDMVGGAPQLPYKEWLQVEACRAKLPEMRATLNDLVKQVEE
                     LKKK"
     gene            complement(13014..13532)
                     /locus_tag="PLF77_06395"
     CDS             complement(13014..13532)
                     /locus_tag="PLF77_06395"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPC08518.1"
                     /translation="MKKFFYLTAIMTMLVFAWSANVLAADKIGFINLQEIMQTSNAGK
                     KAADEFKRFYEKETQEIKSMENQLKKMKDELEKQGSVLTQSSRSEKEAAYQKKMRDYQ
                     LLVNDTNEELKKRDQEMTQKLMPGIIKIVRSIAEREKYTLVIDVATMPIPYYAKENDF
                     SNKVIEEFNKTK"
     gene            complement(13581..16226)
                     /gene="bamA"
                     /locus_tag="PLF77_06400"
     CDS             complement(13581..16226)
                     /gene="bamA"
                     /locus_tag="PLF77_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HPC08519.1"
                     /translation="MNTSKKSFKISLALLLFVFLFVPPAYSEDLKKIVVMPFEVFSNQ
                     DKTVIRESFYKNLQAELQKEKSIQIIAADDFLQREGQIDENTALDHGKAKGADFIVTG
                     TLTQFGKSMNIDCKIFDVEKQSSLPAVSTHGRGMASTTALAEQLKVEILDRIGIVQKI
                     ARIEIQGNRKINASAITTKLKSRKGGIFSEADIAADIKTIFKMGYFLDVTAETDATPE
                     GKVITFFVQEKGLVSEIVINGNKAVSKDDIQEVLTIKTRQSLNREQIKADIEKIKALY
                     DSKGYYNAEIKDRVEQDGETDFRVVLEIRENDRLYIRSIAFEGNEAFPAKELKKLMST
                     SEKGFFSFITDSGLLKRDQLKQDIGKINAYYFNNGFINAQIGEPETTADQKGIHVKIK
                     IQEGKRFKVDNVAISGDVLEKPREALLQSLKVKKGDNYNREAIIRDLDLLTQACNDEG
                     YANAEINPKINTRENEQLVDVDFQIAKGEPVYISNINISGNSITRDKVIRRQLDVVEG
                     DLYSSSKLRSSYANLSRLRYFEEVDFQTEKGTDKNKMDINVRVKEKSTGMFMVGAGYS
                     ASDKLVMMAQITQQNFLGYGQVLSLKASLGSETSNYDLSFTEPWLFDLPLWCKADIWK
                     YEKEYDYYTIDTQGAGLTLGYPLIERIVGYLGYKYYIDDIREVSPIATDYVKAQEGET
                     SSSAVTLSLIRDTTNDYIFPSKGTKATVSVTHAGGPLEGDANYTQYGASLFAYFPLPL
                     DVVFAAKGRIGYIQDHEGVEIPVSNRYVLGGINSLRGFRYVGPTNGGTSDVIGGTNMM
                     VFNVELVLPFIKESGMKLVAFYDAGNSWDNKYDMGDLRQSVGAGIRWYSPIGPLRLEY
                     GHVLDRGELNDDSKGRWEFTIGMPM"
     gene            complement(16213..16884)
                     /locus_tag="PLF77_06405"
     CDS             complement(16213..16884)
                     /locus_tag="PLF77_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC08520.1"
                     /translation="MIVLENLSKTFFKAGNKIEVLRGLNFKIEKGQSLAVLGASGAGK
                     STLIHIMGMLDHPTGGLLLLDGRNVFAWEEKKRAAFRNSHIGFVFQFNNLLPEFNALE
                     NTMMPALIGGFNKKQAGEKACKLLQEVGLESRITHKPGELSGGEQQRVAIARALIMEP
                     DILLVDEPTGNLDTETGKKIEDILVNLNVTKKITLVTVTHNKLLAERMSERILLRDGE
                     IYEYQ"
     gene            complement(16909..>17579)
                     /locus_tag="PLF77_06410"
     CDS             complement(16909..>17579)
                     /locus_tag="PLF77_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPC08521.1"
                     /translation="ESGFYEYDSTLAYLSLPSCQKFLQMGDTVTGIDIKVNDIYKADV
                     IARKIQSALGFPYWTQNWMQMNKNLFSALKLEKRVMFIILSLIVLVAAFNIISALIMI
                     VMEKNKDIAILKSMGATSASIMKIFVYQGLIIGVIGTFLGCAAGLAVALNLQKVSLFV
                     EKIFNFKFLPGDVYYLSELPSKVNYSDVAIIVAGTLLICFLSTIYPSLRASKTDPAEA
                     LRYE"
BASE COUNT         4003 a         4402 c         4287 g         4887 t
ORIGIN      
        1 ttcccggtag cgcagttcga tatcgtgcaa tttgtcccgt ataatatcca tgttttatgt
       61 cttcaataat aaatgtaatg attaaggcgg tttgggttac tttgacaggc ttccttcctg
      121 agttttgaag gaaacctata aatggcgatg cagggaggcg tttaacaacg tttctgaaag
      181 ataaggaaag ttgttcaaaa tcaggcaaac atgtttcttg gcatgatcaa gacaaaattc
      241 cggatcgagc agcgtgcagg aaaaatgctt tttcttcatt cgaggatgtt gcgacatgaa
      301 ggttcttctc tgataagatg ttattttatc aagatttttt cttcttggac gtcgttgcgt
      361 caactttggt ttcgtatttt ttcttgaatc tttccacacg gccggctgtg tcgacaatct
      421 tctgcttgcc ggtcataaag ggatggcaat tggaacagat ttccgttttg atgtcctttt
      481 tggtggatct tgtatggata acattaccgc aaacacaggt aattgttgtt tcctgatagt
      541 caggatgaat gccctctttc atgatactta ttttttcctc cctgattttt tatctataat
      601 taatcattag tgttttttgt cttcttggcc aaacgttgcc ctgccttaca atatatggaa
      661 caaaaattca agtaaaaata gaaaacattt ttatgcggct acgaattcat tgaatccaga
      721 aattcctgat tgttttccgt tttcttgatt ttgccgacaa tgaattccat ggcgtcgacg
      781 ggattcattt cctgcaacag ccggcgcaga atccatacgc gattgagatt gtttttgggg
      841 atcagcaatt cttcttttct ggttccggaa cggatcaggt cgaaggcggg gaagatcctg
      901 cggtcggaaa tacggcgatc caaatgaagt tccatgttgc ccgtgccctt gaattcttca
      961 aaaatgactt catccatccg gcttccggta tcaatcaatg ccgtggaaat aatggtcagg
     1021 ctgccgccgt tttcaatatt ccgggctgcg ccgaagaagc gtttgggttt atgcaaggca
     1081 ttggaatcca cgccaccgga aagaaccttg ccgctgggcg gaacaaccgt gttataggcg
     1141 cgcgccagac gggtaatact gtcgagcaat atcaccacgt cttttttatg ttcgaccaga
     1201 cgtttggctt tttcgataac catttccgcc acctgcacgt gacaggtcgc cggctcatca
     1261 aaggtggaag agatgacttc gccggaaacg ctgcgctgca tatccgtaac ttcttccgga
     1321 cgttcatcga tcaggagaac cattagaacg acttccggat gattggcggc aatactgtgg
     1381 gcaatatcct gaagcagaac ggttttaccg gttcgcggtg gagaaacaat caatcctcgc
     1441 tgtcctttgc caatgggcgc gaacagatcc agtgttctgg ttgagtaatt cttcggatca
     1501 tattccagat ttaatttttc ctgcggatac agaggggtaa ggttatcaaa aaggatttta
     1561 tcacgggcct gttccggact ttcaaaattt accgtatcga cttttaaaag ggcgaaatat
     1621 ttctcgcctt ctttcggagg acggacttcg cccgaaaccg tatctccggt ttttaaatta
     1681 aatctcctga tttgtgacgg agaaatatag atatcgtcag gactgggcag gtagttgtaa
     1741 tcgatggctc gtaaaaagcc gaatccatcc ggcaaaattt ccagaacgcc ggagcctgaa
     1801 ataacgccgt tttgttccgc ctgcgtctgc aggatggcaa aaatcagctc ctgacgacgc
     1861 atcccgctcg ctcccggcac gtccagtttt ttgcccagtt cattcaactc gctgatgggc
     1921 aatcgcttaa tgtcttcgat gttcataata gttccttgcc ttaatttgga atcgggttaa
     1981 ataaagaaac cctgatattg gatatttgtc tcggctttac tgcattgtgt ttaaagaaac
     2041 aaaataaaga tagctaagat ttttagtaat tacggattta gtttcggctt gtgtatttga
     2101 atacttgaaa cgctccaaag ggtagggcta aatagccttt tctcgttgcc aataaactta
     2161 tatgattata atttcgctgt caagaaaatt tattttaggc cttcaatatc acaaccgtca
     2221 aggaaaaaac atccgcctca gtcaatgttt tttgcttctt ccacaagcat gaccggaata
     2281 tcgtccttga tttcatacag caatttgcac ttatcgcaga taatgccatt ggctttttcg
     2341 tcaaagcgaa ccttgccgcg gcatttgggg cagataagaa tttccagcag ttttttattt
     2401 aacgccattt ttttcctcca tgaccgtttt ttctacggct gcaaaaacct gttcaggtgt
     2461 tatatggtcc atacattttt tttccggaca tgtcttcaaa aaacacgggc tgcaatcttc
     2521 tccggatcgg atgaccgtgt gtcttgatcc atagggcccc gtccgggcag ggtccgtcgg
     2581 tccgaaaaca gcaattaccg gtgttccgac tgcggcggcc aggtgcatag ggccggaatc
     2641 tgtggtgacg accgctcgcg ctttcttata aataccggca agacaaatca gggttgtttc
     2701 cccacccagg tgcaggcctg cggtggtcat taaagcggtt attgcttcca gcggcttttt
     2761 gtcgctgccg gtaaacacca cgttcaattt cagccggttt tgaatcaggt cggccaaccg
     2821 ggcgaatttt tcattgctcc agagttttgt atcccatagg gcaaccgggt tgaccgcgat
     2881 gaattttttg tcttccaggc ggtgtttctg catgagcagt tgcgcttttt tttcttcctc
     2941 gtcgtttgac ggaagaacga acctggcctc cggcgttttt gcgccaaggt gccgcaaaaa
     3001 atccagatag cggtcaacag cgtgtttatc catgtcttcg ggaatctttt cattaagaaa
     3061 caaaccgctg agttcctgcc aggaatcgta tcccagtttc cttctgcctc tgctgagaaa
     3121 gacaatgatt gcgcttttaa aaagcccgtg aaaatcaatg accagatcat aatggcgctt
     3181 tcgcaggcct ttgataaagg agatgccttc gttgagggac tgtctgattt tcccggcccg
     3241 gacattttgt atccaagtct tgcggcgtga aatcaacacc tcgtcgagac aggggtgatg
     3301 tttgaccaga tcggcggccg cttcttccac cacccaggtg atatgggcgt cgggatagag
     3361 ccggcgcagc tcggccagag acggaagcgt gtgaatcaca tcgcctattg cgcttaattt
     3421 tacaatcagg atgttcacgt tttgtccttc aatatccact gtaccgcctc gagaatgtcg
     3481 gcggcaataa aatcaggttc gctctccggg gttggcctgt ccggtccgtc gtcctggagc
     3541 aagtcgtttc cgtagcctgt tttcaccaga atacctttca ccccggctcg tctggcggcg
     3601 atcatatcga tgtatcggtc gcccaccaga taagaaaggg taagattgat atttaaatcc
     3661 cgcgccgcgc gcagcagcat tcccggagcc ggtttgcggc aatcgcaaat ttggcggtaa
     3721 ggcgctttgc cttcggaggg atgatgggga cagtaataaa aatcatcaat agaggctccg
     3781 tgtgttttga gaattttctg taaatggctg ttggcggttt gcacgaattc ttccgtgaac
     3841 aatcctcggg cgacgccgga ttgatttgaa ataacgatgg ctttcatgcc gctttgatta
     3901 atcatctgaa ttgccttaca ggcgttcgga atcactttga gtttttcaag gccgtccagg
     3961 taaccgacct cttcattgat aaccccgtct ctgtccagaa aaaccgccgt attctttttc
     4021 attgatcttt atcccggtta tccttcagca atgtttgagc gaccgacagg acatcctcca
     4081 cggtaatcaa gttcatgcag cggaaatccg tcggacaggt ttttttcagg caggggctgc
     4141 aggaaacttc cttgcggacg atcaaggatt gctttcccac aggcgacgtg gttgtcggat
     4201 tggttgaccc gaatatcgcg atcgtgggaa cattgagcgc gcctgccaca tgcattaatc
     4261 ccgaatcatt gctgataaaa agacggcatt gagaaatcag ataaatcgcc tcccgcaaag
     4321 aggtttcgcc ggccagattg atcatatcgg cgcgggccga tgtccggacc tcctcggccg
     4381 ttattcggtc cgctttccct cccagaataa ttccctgcac agaaaattgt tcatccagcc
     4441 ggttgagcgt ttccgcaaaa cggtccggga accatttttt ggcagcgcca taggtggcgc
     4501 ccggtgctat tccgatcagc attttctgcg acggtgcgac atagcggcgc aaaacgtttt
     4561 gtgcctcctc gcggctgatt tttgtttcca gattcatgtc ccggttcgcg gaaacgcagc
     4621 ccaatgcctt aaccatttcc agataatagt tcacctggtg gacctttttt atgtcgcggg
     4681 taaggggcac gctgtgcgtc agcaaaaggc cgcgggcatc cgaattgtaa cctgcccgga
     4741 cgggaatcct tgcaaaaagc gcgatgatgg cagcctctat ggcattctgc aggaggatgg
     4801 ctgcgtcaaa ttgcttttcc cggagcatcc gggcgagccc gaatacgccg gtggccgtgt
     4861 catatttgtt ttcatagacg atgacttcat cgaggttgga aaaaatgtca taaacatccg
     4921 ccacccacgg cttgacgagc atggcaatcc gggcctgcgg ataagtggcg cgtatggagg
     4981 ctacggcagg caaggtcagt attgcatctc cgatccagtt ggtgccgcga atcagaattt
     5041 tatggatgcc tttttggggc agttttttga ttttttcgtc tttcaaagaa cccccttcat
     5101 ccctttttcg tcccggcccg gatcttgtgt aagataaaat tttcaaacga ctgcggattg
     5161 tctttgatct ccattgttat gcgcaggacg aaaagcattt ttgacatttc cggatgattt
     5221 tttaaacgca tcgcatcctt ttctgtcgtg atcagatagt ccgccgcgct gtttttaaaa
     5281 tcgtttttga ttttttccag ttcgtgttcc tgaaaacggt gatgatccgg aaaaatatta
     5341 aacgcaacca aaacggcttt ggttgacagc agcgtctttt taaacgactc cggattggct
     5401 attccgcaaa acgcgtggac cctttttcct tccagctcag taaacggttt acaaattttt
     5461 ttatccgcgc tgatcatatc gtttatccgg tgaagacttt gaaaaacagg aaggttttcc
     5521 tttgtgatca ggttttctat cgtttgatgg accgcgatct gcccgtcaac gcgtgtaaaa
     5581 acaacggcac tggcgcgccg gagtgctttt accggttccc gcaatctgcc ccgcggcaga
     5641 acatggatgt tgtctttcag atcctgaccg tctattaata ccagattgac atcgcgaaaa
     5701 atctgccggt gctgcatggc atcgtcgcag atcagcacat ccgcgccgaa gtggtcgatg
     5761 gcggttttgc cggttgctgt tcttcggggt ccggtaatga ccggaatgcc cgtcagagca
     5821 tgagccatca gcaacggttc atctccggct atttcgctgt ccagaaggat tcgctgtcca
     5881 tccgatacga tgttcacggc gccttttccg cgtccgccgt agcctcggct gatcacggcc
     5941 ggtttaaagc cccggtgctg aagcatgcgc gccagcatga ccacgcaggg tgttttcccc
     6001 gtgccgccga ccgtgatatt gcccacactg atgaccggac agcccagccg ggtcgctttt
     6061 aacaggttgt gatcgtagag ccagttgcgt aaattgacga tggaaagata gaagaaagat
     6121 aaaagatgcg caataaattt tatcgtgccg taaccgcttg cgcttccgtc gttatcccaa
     6181 atccttggcc agtcggttat tgttttcatg acttccttca tccttgaact gtaaattgta
     6241 cagtcggaaa tattcaccct gtttctgcat cagcgtttcg tgattgccct cttcaacaat
     6301 ttcaccgttc accagcacaa taattctgtc ggcgttgcgg atggtggaaa gcctgtgcgc
     6361 gataatcagc gtggttttgc ctttcatgag gttatccagg gcttcctgaa cttccatttc
     6421 cgcttccgtg tccagcgagg aggtggcctc gtccagaatc agaatgggag cgtttttcaa
     6481 aatggcacgg gcgatggaaa gacgttgttt ctctccgccg gaaattttcg tgccgagttc
     6541 tccaatgttg gtgtcgtacc ctttgggcat tctcatgatg aaatcatggg cattggcggc
     6601 tttcgcggcg cagataatct cgtcgtcgct tttttcgaaa ctgccgtagg cgatattgtt
     6661 tctcaccgta tcattgaaaa gaatggtctg ctgcgtgacg atggcaatct gccggcgcag
     6721 cgacggcagg gaaacgtcgc gaatgtccag accgtcgata agcacgcggc cttcgctgac
     6781 atcataaaat cgcgggatca aattgaccag cgacgttttt cctcccccgc tcatgccgac
     6841 gaacgccaca acttcgcctg cccgtataga caacgttatg tttttcaaaa ccggcgtttt
     6901 ttcataacgg aatgtcacgt tatcgatctc gatgccgcgg tcaatcggcg gcaattcggc
     6961 ggcgtggggt ttgtcctgga tatccggaac caggtcaatg atgctgaata ctctttccgc
     7021 accggcaatg ccctgattga tcgtgttgtt gacgttggta agtcttttca ccggttcgta
     7081 cagcatcagc acagcggtca aaaaggaaaa aaacgtgccg ggtgtggagt tgccggccag
     7141 aacgttgtag ccgccgtaga agatgacggc cgcaataccc agtccgccca gaaactccat
     7201 caggggatgg gagagggctt tcacggaaac ggccttcatg ttcaatttaa aaaagcgctc
     7261 gttctcgccg gcgaatcttt tgatttcgtg cgtttccatg ccgaaggcct tgacgatgcg
     7321 cgttccggca atggtttcct gcaaaagcga gctgagattg gccatggtga tctgcgtcga
     7381 cgtggtgacc ttgcgtattt tcttcccgaa tgcggaaata ggataaatcg tgagaggaaa
     7441 aataatcatg gcaatgaacg ccagcttcca gtccgtataa aaaatgacac agatcagggc
     7501 gacaattgta aaggaatcct tcacgatact ggtgaccaca tcggacaccg cgttttgaat
     7561 ggacgcgaca tcattggtaa tgcgcgacat gagcagcccc gtcggatgat gcgtaaaaaa
     7621 ggacagcgac tgtttctgta tctgttcgta gagctgattg cgcagatcgg tgacaatgcg
     7681 caagccgata aaattcatta aaaccgtttg aacgtaatta cagattcctt tcaccagaaa
     7741 aatcatgacc acggcgagag ggagccattt cagcgacgcg gggttttttg ccagaaagat
     7801 gtcgtccagc acaggtttca ccagcaccgc ctgtgacgat gtcagtgcac cggctatgac
     7861 catacagagc atggaaatca gaaaacgcac gctgtatgga cgaccaagac ttaaaagacg
     7921 tttaaatatt ttcatgatgt ttccggtgtt cctataacat atctaatgcc agtttagcag
     7981 ttttaattgc cgccccgggc tcgccgagcc gggaccggat agcctccagt tcccggatca
     8041 tggcctgctt ttttgtttca tcgcctaaaa tggaaagggc ctccctggcg atccgggcgc
     8101 cgctggcatc gttctgaatc agttccggca caatggtttt ctcggcaatg atgttgacga
     8161 ggccgatatt tttgacgcgg acgaataatt ttccgataaa ataagacagc ggcgaaattt
     8221 tgtaaacgat aatcatgggc actttcagta acgctgtttc cagcgtggcc gttccggagg
     8281 cgaccagcgc caggtctgca caggagacga catcgtaggt ctggccggaa atgacgttga
     8341 catcgatcgg aaatccggca atgattccgg ttagattttt ctcttcaagc gtgtcggcca
     8401 atgggacgac aaattgcagc tccgggatgc tgtctttcat gattgtggcg gcctgaagca
     8461 gttccggcag caattttttt atttccgaca tgcggctgcc cggcagaatg cctatcgtga
     8521 tcttatcttc gtgcagatcg aacttttttc tcgattcttt ttttgaatag gttgtcttaa
     8581 tcatatccag caggggatgc cccacataat ctgccgcaaa accgtgccgc cggtatgttt
     8641 ccacttcaaa aggcagaatg accgccatcc gggtgacggt ctttttgatc tgttcgattc
     8701 tgctctgacg ccaggcccag acctgcgggc tgatgtaata taaaaccttg atgttgtgcc
     8761 gacgggctgc tttggccacg ggcagattaa aatccggata atcaatcagg acgacaagat
     8821 ccggacgccg ttcatcgagg gattttttta tcgtcctcat gattttcaga atgtctttta
     8881 atttgaaaac gacctcggtc aggccgacaa ccgccatcgt ggaggcgtcg gcaagaagct
     8941 gaaccccttc ctgcagcatc tttttcccgc cgatgccgta aaatttcagc gccggatcgc
     9001 gattcagcat ttcccgcaca agattggcgc cgtgcatgtc gccggaggct tcgccggcga
     9061 tgatcaacac ggttttattt ttatgatttg aatctttcat gatctcagcc ggatttttct
     9121 tcaggatgcc ttgttaatga tatcggcaat gaattcgatt tctttctgat ccaattcagg
     9181 gaacatcggc agggaaagaa cttcccgggc gcatgtttcg gattgcggca tcgtttccgg
     9241 tccgtcgtac aggttgagaa atgcgttctg ccggtgaagc ggcacaggat aatacacggc
     9301 ggaagcgacc tcttttttct gcagcgcgtc ggccagaaga tcccgctcgt ccgagcgaat
     9361 ggtaaactga tgatagacat gttcgcaatc gccggaagcg gacggcaaaa cgatgtcctt
     9421 gcgtttgatg gctgcgcaat aaagcgccgc attgcgccgg cgggccgcgt tgaattcgtc
     9481 gatttttttc agtttgatcc ggatgatggc ggcctggatc tcgtccagac ggctgttgta
     9541 gccgacctca tcatggtagt aacgtctggc gctgccgtga ttccgcagca tttttatctt
     9601 tgcagccgtc tcgtcgcttc gggtgacgat catgccgccg tcgccgtaac cggcgagatt
     9661 cttgctgggg aaaaagctga aacaaccgat atcgccgatc gcccccactt tccggccgtg
     9721 atagaccgct ccaaatgcct gagcacagtc ttcgatgacc cggagattat gttttttagc
     9781 gatggccata atgggatcca tatccgcggg atggccgaac agatgaaccg ggataatggc
     9841 tttcgtccgc gaggttatct tgccttcgat ttgacgccaa tccatgttga acgtgacggg
     9901 ctcaatatcc acaaaaaccg gccgggcttt caaaagagcg acgacctctg ccgttgcgat
     9961 aaaggtaaag ggcgtggtaa tgacctcgtc accggcttca atgccgctgg cccgcaaagc
    10021 cagaagcagg gcatccgttc cgttggccac gcccaccgcc tgggacggac cgtgataggc
    10081 ggcgacttct tcttccagcg cggacacgtt gggcccgaga ataaagcggg tatcttccag
    10141 aacattgctg atggcggcat caatgtccct tttgaggctg tggtactgtc tttttaagtc
    10201 gaccatgggt atcatgtcag taaatcctct gctgctttca tttttgtgat gatgtccacg
    10261 gcgagcgcca gggatttgcg cgcctcttcc ccggaaaccg gcgggcagcc gcgggtgatg
    10321 acggcgtcaa caaaagagcg gatttcctct tccagcggat cgtggctgcc tatttcaaga
    10381 tggttttgga caatctgcat ctggcccgag ccggttgtcc ctttgctgaa agacaggact
    10441 tcccgtttct ggcaatcgac cgaatgatag ccttctggac tgaaaaaccg tattttttgc
    10501 atggtcttgg cgctgatccg gctggcggta atattggcgg tgcagccggt ggaaaaagta
    10561 atccgggcgt tggcaatgtc aattttatcc gagagaatgg gaacccccat ggcctccacg
    10621 ctgatcgccg gagcatccac gaattttacg aggatatcca gatcgtgaat catcagatcc
    10681 aggatgatgt ccacatccgt gccgcgttcg aagaacgggt gtaatctatg ggattcgata
    10741 aaaagaggtt tccggatgac gttttccagc gccatgatgg cgggattgaa tcgttcaaca
    10801 aacccgacct gcagaatgag tttctttttc cgcgccagac cgatcagatc atccgcttcc
    10861 gcaagcgttg cacaaagcgg tttttcgatg aggacatcca cgcccgccgc cagaaaatcc
    10921 ctggcgacgt tgtaatggtc gcttgtcggc acggcaatgc tgacggcatc cacgctgccg
    10981 atcatttccc ggtagtcgct gaaggccgcg cactgataaa tctgggctgc tttttgagcg
    11041 cgctccgaag acgtgtcggc aacggcgacg atttcgcagg gttccagttt ccggtatttt
    11101 tgcaaatgat aattgcccag gtggccgatt ccgatgacac cgactttaat ttttttatgc
    11161 ttcatattct atcaatcaac ggcaaatgcc tctttccgac tctttaataa attttatgaa
    11221 atgccttact tcgggcagat cctcaacttc agattccgct tttttgatcg cgtcttccag
    11281 aagcagcttg gaacgaaaaa caatgcggta ggcatcactg atggccttga tggttttttc
    11341 cggaaaattt cttcttttca atccgataag gttcaatccg taaagtttgg catgattgcc
    11401 ctgagcaatc gtatacgggg gaatatcctt tacggcggcc gagcatccgc cgatcatgca
    11461 gtgggctccg acgcggcaga actggtgcac gccggtcagt ccgctgataa tggcgtaatc
    11521 ttcgacatga acatggccgg ccaacgttgc cgcattggac ataatcacgt gattgccgag
    11581 cttgcaattg tgcgccacgt ggcaataggc cataatcaga ttatgattcc ccatgatggt
    11641 gacgccgatg tcggctgttg tcgagcgatg aatggtcaca aattcgcgga tcgtgttgaa
    11701 attgccgatc accacccggg ttttctctcc gctgaatttc agatcctgcg gaggcgctcc
    11761 gatggagcaa aactgaaaga tccggcagct ttcgccgatt tgggtataat cgtcgatgac
    11821 cgtatgcgga ccgatcaccg tatttttccc tatcctgaca tcggcgccga tgatcgagta
    11881 ggggccgatt tccacgcctt cttccagttg agcgccgggt gcaataatag ctgtttcgtg
    11941 aattttcatc agttaacctt ctttaggaga atttactttt tcttgagctc ttccacctgc
    12001 ttcacaagat cgttcagcgt cgctctcatt tcaggcagtt tggcgcggca ggcttccacc
    12061 tgcagccatt ccttatacgg caactgcggt gcgccgccca ccatgtcgcc ggatttgatg
    12121 tctttgtgaa tccccgcccc ggccgcaatc atgacatggt cgccgatggt tatatggccg
    12181 accataccgg tctgtccgcc gaccatcacg cctttcccca gccgcgtgct tccggaaatg
    12241 ccgacctggg ctgtgatgac cgaattttca ccgatgacca cattatgggc aatttggacc
    12301 agattgtcga ttttgacgtt gcgttgaatc caggtcctgc ccagagtcgc tctgtcaatg
    12361 gtcgtgttgg ctcccacctc cacatcgtca tcgatttgca caaagccaac ctgcgggatt
    12421 ttaacgttgt cgcgtcccgg cgctgcaaag ccgaagccgt cggcgccaat caccgccccg
    12481 gaatggagaa tgacccggtt gccgatgatg caggaatggt aaacggtgac gttggcatgg
    12541 atgatggaat gttcgccgat ggatgcgttt ctgccgataa agacgccggg gtaaataacc
    12601 gttcccgcac cgattttggc tccggctgaa ataaaagcac ggggaaaaat aacggcgtcc
    12661 ggagaaacat cggcgccgtc ttcaatgcag gcctctgcgc tgaccccgga ccggccgtgt
    12721 tgcgccggat aaaaatggct cagtattttt cccagggcca cataaggatc ggaaacgaca
    12781 atcaggtttt tcccctccgc cgttgtctgc ggagaaacga gaatcgcgga cgcttttgtc
    12841 gttttcagtt tttttatgta tttcttattg gcaataaacg tcaggtcgcc cggaccggcc
    12901 tcatcgatag cccggatatg gccgatgaca acgtcatccg cgcccataac agtgccgcca
    12961 agaaacgcgg ctatttctcc agtcgtcatt tgcatcggtt gaacgtgccg gtgttatttg
    13021 gtcttgttga attcttcgat cactttattg ctgaaatcgt tttccttggc atagtaagga
    13081 atcggcatgg tcgccacgtc aatcaccagc gtgtactttt cccgttcggc aatggagcgg
    13141 acgattttaa taataccggg catcagcttc tgggtcattt cctgatcgcg tttttttaat
    13201 tcctcattag tgtcattgac cagcaactga taatcccgca tcttcttctg gtaagcggct
    13261 tccttttcgc tgcgtgagct ctgggtcaga acggaaccct gtttttccag ctcatctttc
    13321 atttttttca gctggttttc catgcttttg atttcctgtg tttctttctc gtaaaatcgc
    13381 ttgaattcgt cggcggcttt tttgcccgca ttggacgtct gcatgatttc ctgcagattg
    13441 atgaatccga ttttatcagc agctaaaacg tttgcactcc atgcaaaaac cagcattgtc
    13501 ataatcgccg tcaggtaaaa aaattttttc atttgtggtc tcctcattta aattatatgt
    13561 ttaatttttt atctatgctt ttacatcggc atgccgatgg taaattccca gcgtcctttg
    13621 gaatcgtcgt ttaactctcc ccggtcaaga acgtgaccgt attcgagccg cagcgggccg
    13681 atcggtgagt accagcggat tccggcgccc acggattgcc ttaaatctcc catatcgtac
    13741 ttattgtccc acgagttgcc ggcatcgtaa aaggcgacca gcttcatgcc ggattccttg
    13801 ataaaaggca ataccagttc aacattaaaa accatcatat tggtaccgcc gatgacatcg
    13861 gacgtcccgc cgttggtcgg tccgacatag cggaagccgc gcagagaatt gattccgccc
    13921 aaaacgtatc tgttggaaac gggaatttca accccttcat gatcctgaat ataaccgatc
    13981 cgtcccttgg ctgcgaaaac aacgtccagg ggcagaggga aataagcgaa gagacttgct
    14041 ccgtactggg tgtagttggc atctccctct agggggccgc ccgcgtgggt gaccgaaacc
    14101 gtggccttcg ttcccttgga ggggaaaatg taatcgtttg tcgtatcgcg aatcaaagac
    14161 aaggtgacgg cactggaaga tgtttcgcct tcctgtgctt tgacgtagtc tgttgcgatc
    14221 ggcgaaacct cccggatatc gtcgatataa tacttgtagc ccagatagcc gacaatccgt
    14281 tcaatcaggg gataacccag cgtcagaccc gcgccctgcg tgtcgatggt gtaataatcg
    14341 tattccttct cgtatttcca gatatcggcc ttgcaccaca gcggcaggtc gaaaagccat
    14401 ggttccgtaa aggataaatc gtaattgctg gtttcggagc ccagcgaagc tttcaaactc
    14461 aatacctgcc catagccgag aaaattctgt tgcgtgatct gtgccatcat gacgagttta
    14521 tccgatgcgc tgtaacccgc tcccaccatg aacatgcccg tgcttttttc cttgacccgg
    14581 acattgatat ccattttgtt tttatccgtt cccttttccg tctggaaatc cacctcttca
    14641 aaatatctca gcctgctcag attggcatag ctgcttctca gtttactgct ggaatagaga
    14701 tccccttcca ccacgtccag ctgacgacgg atgaccttgt cccgggtgat gctgttcccg
    14761 gaaatgttga tgttgctgat ataaaccggt tcccccttgg caatctggaa atcaacgtca
    14821 accaattgct cgttttcccg cgtattgatc ttgggattga tttccgcgtt ggcgtagcct
    14881 tcatcgttgc aggcttgcgt cagaagatcc aggtccctga tgatggcttc ccggttgtaa
    14941 ttatcgcctt tcttcacttt gagggactgc agcagcgcct cgcgcggctt ttccagaaca
    15001 tcgccggaga tggccacatt atcgacttta aacctcttgc cttcctgaat tttaatttta
    15061 acgtggatgc ctttctgatc ggctgtggtt tccggttcgc cgatttgagc attaataaat
    15121 ccgttattaa aataataggc attgattttt ccgatatcct gcttgagctg gtcacgcttg
    15181 agcaacccgg aatcggtgat gaacgaaaag aatcctttct ccgaggtgct catcaacttt
    15241 ttcaattcct tggccggaaa ggcttcattc ccttcaaacg cgatcgatcg gatgtagagc
    15301 ctgtcgtttt ccctgatctc cagaacgaca cggaaatctg tttccccgtc ctgttccacc
    15361 cggtctttga tctccgcatt ataatacccc ttgctgtcgt agagcgcttt gatcttttca
    15421 atgtctgcct tgatttgttc ccgattcagg ctctgccggg ttttgatggt taagacttcc
    15481 tgaatgtcgt ctttgctgac ggccttgttg ccgttgatga cgatttcgga caccagccct
    15541 ttttcctgca caaaaaacgt gatgactttg ccttccggcg tggcgtccgt ttcagcggtg
    15601 acgtccagaa aatatcccat tttgaaaatg gttttaatat ccgccgcaat gtcggcttcg
    15661 gaaaaaatac cgcctttcct gcttttaagt tttgtcgtga tcgcggaggc attgattttc
    15721 cggttgcctt gaatttcaat gcgggcaatc ttctgcacaa tgccgatgcg gtccaggatt
    15781 tcgactttta attgttccgc aagagcggtt gtgctggcca tccctcggcc atgcgtggac
    15841 acggcgggca gtgaactttg cttttccaca tcgaatattt tgcagtcgat attcatggat
    15901 ttgccgaatt gagtcagggt gccggtgacg ataaaatcgg cgccttttgc cttgccgtga
    15961 tccagggccg tattttcgtc aatctgcccc tcgcgttgca gaaagtcgtc ggcggcaata
    16021 atctgaatgg atttttcttt ctgcagttct gcctgcaggt ttttgtaaaa agattcccgg
    16081 atgaccgttt tatcctgatt gctgaatact tcaaagggca tcacaacaat ctttttgaga
    16141 tcttccgaat aagccggcgg aacaaaaaga aagacaaaca ataaaagagc caaagatatt
    16201 ttaaagcttt ttttactggt attcataaat ttctccatcg cgcagaagaa tcctttcgga
    16261 cattctttcc gccagtaatt tgttgtgtgt taccgtaaca agcgttattt tttttgtcac
    16321 attcaggttg accagaatgt cttctatctt ttttcctgtt tccgtgtcaa gatttccggt
    16381 aggttcgtca acgagcagga tgtccggctc catgatcagg gcgcgggcaa tggccaccct
    16441 ttgttgttcg ccccccgaaa gttctcccgg cttgtgagtg attctgctct ctaatccaac
    16501 ttcttgaagc aatttgcaag ctttttctcc ggcctgtttc ttgttgaatc ctccgatcag
    16561 ggccggcatc atcgtgtttt ccagcgcgtt gaattccggt agaagattat tgaactgaaa
    16621 cacaaatccg atgtgcgaat tacggaaggc cgcccttttt ttttcctccc aggcaaaaac
    16681 attcctgccg tcaagaagca gcagcccgcc cgtggggtga tcgagcatgc ccatgatatg
    16741 gatcagggtg cttttcccgg cgcccgaggc gcccagaacg gcaagggatt ggcccttttc
    16801 aattttgaaa ttcagaccgc gcagaacctc gattttgttt ccggctttaa aaaaggtttt
    16861 tgataaattt tccagtacta tcatcgtttt gcgtcgtctg tcgggttatt attcatatct
    16921 taaagcttcc gccgggtcgg ttttggacgc ccgcaacgac ggataaatgg tcgataaaaa
    16981 gcaaatcagc aacgtgccgg ccacgataat ggcaacatcg ctgtagttca ctttggacgg
    17041 aagttcgctc aaataataaa cgtctcccgg caaaaattta aagttaaaaa tcttttccac
    17101 aaacaacgaa accttctgca ggtttaatgc gacggctaat cccgctgcac atcccagaaa
    17161 agtaccgatc acaccgatga tcaatccctg ataaacgaat attttcatga tgcttgcgga
    17221 ggtcgcgccc atcgatttca atatggcgat atccttgttt ttttccatga cgatcatgat
    17281 caaagcgctg ataatattaa aggcggccac cagcacaatc aggctcagaa tgatgaacat
    17341 cacccgtttt tccagcttca gcgccgaaaa aagattcttg ttcatctgca tccagttttg
    17401 cgtccagtag gggaatccca gagcgctttg aattttcctg gcaatgacat ccgccttgta
    17461 aatgtcgttc actttaatat caattcccgt taccgtatct cccatttgca gaaatttctg
    17521 gcagctcggc aaagacagat acgccagggt tgaatcgtat tcgtaaaatc cggattcaa
//
LOCUS       DAOOLB010000039        16948 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_3543_length_16948_cov_44.138045, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000039 DAOOLB010000000
VERSION     DAOOLB010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 16948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16948
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3543_length_16948_cov_44.138045"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..801
                     /locus_tag="PLF77_06415"
     CDS             211..801
                     /locus_tag="PLF77_06415"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPC08522.1"
                     /translation="MAITLPELPYAKDALAPVISANTLDFHYGKHHKAYVDNLNKLIA
                     GTDLDQKSLEEIINIAAKDSAKAGIFNNAAQVWNHTFYWKCLKKTGGGRPSGAIAAKI
                     NEMWGNYDKFVEELKNAGATQFGSGWVWLVSDKTRLQITKTANADTPLAHGQKALLTI
                     DVWEHAYYLDYQNRRPDYLSAVIQNLINWDFVNANL"
     gene            complement(886..1167)
                     /locus_tag="PLF77_06420"
     CDS             complement(886..1167)
                     /locus_tag="PLF77_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPC08523.1"
                     /translation="MNKSDLIEALSTKLNLTEKKAIDVVNLIFKGFTDELKKNGRIEI
                     RGFGSFVVKKYGSYTGRNPKTGSKIQVEPKKLPFFKVGKELKVRVDKKK"
     gene            complement(1279..2007)
                     /locus_tag="PLF77_06425"
     CDS             complement(1279..2007)
                     /locus_tag="PLF77_06425"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HPC08524.1"
                     /translation="MKERLQKVIAAAGITSRRHAEELITRGRVSVNGVVVTKLGEKAD
                     AQKDIIRVDGKIISTEKVKYYIVLNKPAGFVTTLHDPQNRPTVVDLLGSVPERVYPVG
                     RLDYDSKGLLLLTNDGDFAQQIQHPRFQKPKIYKVKIQGHLSRQDVKQLSRGINVDDD
                     FFKPENLKVDKMNDRSCWLRLTLKEGKNRIIRRGFEKIGYRVALLVREAIGDLALGAL
                     KEGEWRHLTRKEISQLLDSAVKSK"
     assembly_gap    2090..2189
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2190..2824)
                     /gene="scpB"
                     /locus_tag="PLF77_06430"
     CDS             complement(<2190..2824)
                     /gene="scpB"
                     /locus_tag="PLF77_06430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HPC08525.1"
                     /translation="MEKIKAVIEALIFASDTPLSPERIRAVFPEVEKTEIREIIDQLV
                     SEYHDRQGGILLQEVAGGFQFRTNPDLSLWIKKLKTAKPQSLSPQALETLAIIAYKQP
                     IVKSEIEDIRGVDVGGPLKGLLEKKLIRIVGRKDVPGKPIIYGTTRKFLEVFNLKDIM
                     DLPNIRELKELHHQQELLDPDQESSESEQETIIEEEAVAKQHDDENAEEKI"
     gene            complement(2825..3601)
                     /locus_tag="PLF77_06435"
     CDS             complement(2825..3601)
                     /locus_tag="PLF77_06435"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HPC08526.1"
                     /translation="MDNNNLGHPMDPMDNEQRLNYAIKLDIFEGPLDLLLYLIKKNEI
                     DIYNIPIALITEQYFQYLKMIKALNLDLAGEYLVMASTLIHIKSRTLLPPPEEPEEEE
                     EDPRAELVRQLLEHKTFKEIAVSLGNRPLLERDVFTRAAALPEVEIKSTDEEEEFIEV
                     SVFELIEAFHRIISQMDKKELMEIDLEKLSLTDIINEIMEQLTVTKNITFEDLLKGKK
                     DRRRIIYTFLAILELIKLRMIKAYQTSMFGVIRIFPAVES"
     gene            complement(3611..4600)
                     /gene="trpS"
                     /locus_tag="PLF77_06440"
     CDS             complement(3611..4600)
                     /gene="trpS"
                     /locus_tag="PLF77_06440"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPC08527.1"
                     /translation="MERKRILSGMRPTGKLHLGNLHGALRNWIALQESGQYDCFYFVA
                     DWHAITSEYSSTEGIQDNVINMVIDWLSVGLDPQKSTLFLQSAVKEHAELFLLLSMIT
                     PLAWLERNPTYKEMKTELANKDLSTFGFLGYPVLQAADIIMYKAYGVPVGVDQLPHVE
                     LTREIARRFNFLYREIFPVPEPILTNVPKLLGTDGRKMSKSYDNSIYISDRGKNLQHK
                     ISSMFTDPQRMRKKDPGNPDICNVFTFHGLYSSPEKVKEIDVECRRAGIGCTDCKKML
                     AQRVAENLQPVHERMDYYVSHMDEINGVIEEGNRKAAKIARQTMSEVRAAMKI"
     gene            complement(4884..5156)
                     /locus_tag="PLF77_06445"
     CDS             complement(4884..5156)
                     /locus_tag="PLF77_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPC08528.1"
                     /translation="MTKAELIGVIAKSAGITKDKAGKALDTFQDAVTKELKKSGKLAL
                     VGFGTFSVVKRKAREGRNPQTGKTIKIPAKKVVKFKAGKELAGKVK"
     gene            5568..6488
                     /locus_tag="PLF77_06450"
     CDS             5568..6488
                     /locus_tag="PLF77_06450"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HPC08529.1"
                     /translation="MQSVHFEKIKSWFSLSRPAFHTVGILPFILGTILAYKINLVFDP
                     AVFALGVSAVILIMLSTYHMGEYFDYREDEISQRLYKSRFAGGSGVVPAKTVKRSVAL
                     RTGLISFIIAGIIGLTLQFILKTGPYTLLLGCLGAFPGFFYSTQPVRLVQKGVGEIFI
                     GFCYGWLPVASAYYIQTGFVHSVIHWITVPIGLTIFNVIFLNEYPDYEADKATGKKNL
                     LYRLGKRKGKVLFFVFSLLASAAVFASPVFGVPFKAVYFYLPVAIVSLFIAVMMLRDQ
                     YENRKTMELLCGLNIAVNLGTSLVYILAYI"
     gene            6494..7720
                     /locus_tag="PLF77_06455"
     CDS             6494..7720
                     /locus_tag="PLF77_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08530.1"
                     /translation="MTNVKKKVKAFYNPPSLLLKQLGSSLLMAYLNNWRNYPRWGFQY
                     GFRSIPVANGALGMGCIGYPGHPVWEVTGACNLKCIHCHAVSDKAGQDELSTDEGKRL
                     IDSIASVSAFRTLVYTGGEPLVRPDIFELLRHSQQAGLANIIATNGTLIDEEMAFKLR
                     DHGVVCHAISLDAATPAIHDMVRNKPGAFDRTLRAIKATKKAGILLQINTTAMEYNMP
                     HLSELIDFADRCGAGIMLMYQLVAVGRGEKIEKATLKKSANKHLSEMIAEKQKNATTI
                     IEPVAGPQYWPYLLEKKGFTEGLRLKLAGKVFHGCAAGRGFVYVKANGDVWPCPFVEV
                     NAGNIRDRNFKAIYEESPVFQNLRRREELLKGACGECHYKTVCGGCRGRAHAYSGDYL
                     QEDPRCYIRETTQNHS"
     gene            complement(7839..8996)
                     /locus_tag="PLF77_06460"
     CDS             complement(7839..8996)
                     /locus_tag="PLF77_06460"
                     /inference="COORDINATES: protein motif:HMM:NF015523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HPC08531.1"
                     /translation="MRYSLKILLLILMAFFLGGCMRAVSQPSFKTLDSLIPVDATQVA
                     FSDDLEPASLETAIDRSIRFYEGRGRNNVYQIEDRQIDAQQFKETLLAFRALLGDAKD
                     PETLGKRMAEAFDVYQVTGTDDESRVLFTGYYEPLLEGSLQKTEKYKYPLYRVPPDLV
                     RKGTRIGRMQNDKFVPYYSRREIDVDGVLRGKNLELLWVSDPVDLFSLHIQGSGKIKL
                     ENGDLLTVGFANTNGRSFRSITKFLLDKGTIQPHEVTYRHDFLRGKRDEEIYEALSHN
                     ERYTFFRFLEKDPVGSLGEAVTPDRTIATDPEYFPEGALAFIRLRKPVFDTDGHVKER
                     VEFSRFVLSQDKGAAIKGPGRVDLFCGFGEKAEKTAGTLKERGELYLLLKK"
     gene            complement(8993..9451)
                     /locus_tag="PLF77_06465"
     CDS             complement(8993..9451)
                     /locus_tag="PLF77_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HPC08532.1"
                     /translation="MLRKARIDDVKIIHRLINLSSGKGEMLPRSLMDIYGSLRDFFVY
                     YDEDESRIIGICAMNIIWANLAEIRSLYVEESHRGKGVGRVLVEACISEAITLGLFKV
                     FTLTYKKDFFLKLGFKEIDRNLLPEKIWSDCFRCSKYPDYCDEVAMIVEL"
     gene            complement(9445..11157)
                     /gene="recN"
                     /locus_tag="PLF77_06470"
     CDS             complement(9445..11157)
                     /gene="recN"
                     /locus_tag="PLF77_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HPC08533.1"
                     /translation="MLNELSIKNFAIIDELHVTFGEGLNVLSGETGAGKSIIIGAVSL
                     LLGDRATSEMIRTQADTATVEAVFDISADKPLRDKIDAMGFPVSDELIIRRVISHTGK
                     NKVFINGQAATLANLAAVSERLINICGQHEHQIILAAENHIDILDEFGGCLSERAVYT
                     EHFNQYQQIRSGIEKLEELSKSADARTELYEFQLKEIQDINPQPNEDTVLSDEKKVLV
                     NIQRLSEWAGNAFDLLYGDKNSVIVMLKEVQRQIKEIKKIDAGLKISEAEMENNFVAL
                     QEAALLLRDYGKGLFFDAERLAAIEERLDLIHRLKRKYGGSLEAVFQKKQDMEEALRK
                     VSSLDEELQKMIREEEAVKSRLRQSARNLSESRKKAAKTLEDKVGKEIRALNMPHASF
                     FVHFKNRADDNTDFFGPRGADDIEFDLSANKGEDARPLNKVASGGELSRIILALKNVL
                     SGTGSVLSMVFDEVDNGIGGAVAEIVGRKLRAVSANHQVICITHLPQIACFGDKHMFV
                     SKKVVKGRTLTFVNELDSEQKIEEISRMLGGVDVTPTTREHAREMLQNAGSHNLKVSG
                     GDTC"
     gene            complement(11150..12040)
                     /locus_tag="PLF77_06475"
     CDS             complement(11150..12040)
                     /locus_tag="PLF77_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HPC08534.1"
                     /translation="MKIKKIGIAANIEKEKIADHVRSLKKWLEERGAKVFLETEISGK
                     ILSDGGYSWSELARKAEVAVVLGGDGTILRSARYLAVHRVPILGINMGGLGYLTEVNL
                     NEIHSTMELILKGKFVTEKRMMLDVSVQHGKTVAGIGSVLNDVVMNRGDLSRMNELEV
                     EVDGEYLTTYKGDGLIVSTPTGSTAYSLSAGGPIVFPGKDLILINPICPHTLTNRPII
                     FSEDAHLRITLWSKDKGAMLSLDGQETHKIKSGDVVIVKKSRHATMLILSPYRSYGEI
                     LRSKLGWGDLPSGAHKRKNA"
     gene            12217..13296
                     /gene="rdgC"
                     /locus_tag="PLF77_06480"
     CDS             12217..13296
                     /gene="rdgC"
                     /locus_tag="PLF77_06480"
                     /inference="COORDINATES: protein motif:HMM:NF016281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HPC08535.1"
                     /translation="MGLLKGGFTFSRFSVDGQLPQAFLNFVNNRIRANSFKNSFKSTE
                     EKTMGWVSLTDILDTDFKNANYSLGAYLIFSLRIDRKLIPPKLLKIKLMEEERRFLAE
                     SGKNKIFRQQSTALKDKVKLDLLAKLDAIPFFYDVCWAVEQNTLYFSSLSDKVIDDFA
                     DLFKKTFALNLKRFSPQENHLIRKDADPAESTSLIGREFLTWLWFKSEERNGRIVLPN
                     KEECEIHFLKRVSLEAGEGEYSQGVVCHGIHAELKEGKEAIRQGKKVKEAGIKLLHNN
                     NEWDFMFKADSFDFQSLKMPQTDWQDTPEDPSGSLLERIYLIENAVKTIDTIYESFLH
                     IRQSPQWKEKELKLLHKWLEKNPKD"
     gene            complement(13301..14353)
                     /locus_tag="PLF77_06485"
     CDS             complement(13301..14353)
                     /locus_tag="PLF77_06485"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF009206.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HPC08536.1"
                     /translation="MIKVLVVDDSALVRKVLTEELSRHKDIEVVGGAIDPYVARDKIV
                     RLKPDVITLDIEMPRMDGLSFLSKLMKFYPMPVVVVSSLTPRDSQNAIRALELGAVEV
                     ICKPGSSLSTADLSVRLVEAIRAAARAKIAKPAVELVERESVPGYKIQLKTTHKVIAI
                     GASTGGTIAIEKILSRLPMNIPGIVVVQHMPAYFTASFAERLNALCKIEVREAKDGDT
                     AAPGLALIAPGGYHMLLERSGAFYQVRIKGGPMVHHQKPSVDVLFHSVAKHAGANAMG
                     VLLTGMGADGADGLLAMRQNKAYTIAQDEATSVVYGMPGEAVKLGAACDELPLDEIPG
                     RIITRLSGEDKKREDK"
     gene            complement(14350..14829)
                     /locus_tag="PLF77_06490"
     CDS             complement(14350..14829)
                     /locus_tag="PLF77_06490"
                     /inference="COORDINATES: protein motif:HMM:NF025070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HPC08537.1"
                     /translation="MKTKIEKILEEATIKTFEDICFMYLEPELKDSQAALEPDAAAEV
                     EFHGAYNGRLVIASRGGLFSAIASNILSSDHPSLQEKKDALGEIANIICGNIVPSLGR
                     RKAGGYKIQSPRHLGRDELLKEEKSKPSAVVILNFNQGRADIKLFVDDYISDQEIND"
     gene            complement(14813..15205)
                     /locus_tag="PLF77_06495"
     CDS             complement(14813..15205)
                     /locus_tag="PLF77_06495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC08538.1"
                     /translation="MALNTLVVDDSSVMRTIIIKTLKLAGLPLGDIWEAKNGEEGLHV
                     LQDHWVDLAMIDIHMPVMDGEEMLIKVRENDQYKNLPVIVISSESDPGKIEKMLELGA
                     TFIHKPFTPEVLKEVIVEVTGVSHENED"
     gene            complement(15261..15743)
                     /locus_tag="PLF77_06500"
     CDS             complement(15261..15743)
                     /locus_tag="PLF77_06500"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HPC08539.1"
                     /translation="MVYTVGVADLKISDESADMLITYALGSCLGVTVYDFRKKTGGLL
                     HCMMPDSGIDQNKAAGNPYLYVDSGMKAMMDKFYQKGSRKSDLIIRVAGGSSSKLNEN
                     EDFFQIGRRNFVSLRKYLWNEGLMLKAYDVGGYGSRTVTLELESGKMLIKSQTFLKQL
                     "
     gene            complement(15745..16644)
                     /locus_tag="PLF77_06505"
     CDS             complement(15745..16644)
                     /locus_tag="PLF77_06505"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HPC08540.1"
                     /translation="MHSEWNNLSTDYYSLKLTSEQFDKISRLVYQISGIDLHEGKEEL
                     VKARLIKRLRHLKFHDFNQYLKHIANDKSGTEIGAMVDILTTNKTNFFRESEHLDFLK
                     HEIIPGWVKGPVRIWSAGCSSGEEPYTIAIVLLESIPDTATLDIKILATDISDRMMEK
                     ARQGLYDEESLAIMSRHLKHKYFQKTDTGSGYKYKVIPKIQSMVSFAKLNLMEHWPMK
                     GPFDVIFCRNVMIYFDKSTQEKLVGRFWHLLKEDGYLMVGHSESLSFLQHEYRYMRPA
                     VYQKIRKAHSVVKKTGKVRTDVK"
     gene            complement(16713..>16948)
                     /locus_tag="PLF77_06510"
     CDS             complement(16713..>16948)
                     /locus_tag="PLF77_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08541.1"
                     /translation="AEEMSSQSEEMRAMVAGFRLTKTVALTAGPDAIKKTEYRLPDVA
                     PGKKGDKASAGLHPDPRKVIPLDDKDHDILQNF"
BASE COUNT         4145 a         4302 c         3710 g         4691 t
ORIGIN      
        1 gcaagacgca tagaagacga ctgcggcgat gtgtgaaaaa cctcaaatat caatttgaca
       61 aatttaaaac acgacctatt ataaaatacg aacacgtgtt cagtgtattc cgatgctctt
      121 tgtaatcgga ataagtctgt taagtatttt tcaacttctc ttgaatgcag caagaaggta
      181 aaaatcaact ctcaaaaaag gagaataatt atggccatta cattgccgga attgccctac
      241 gcaaaagatg cgttggcgcc cgtgatcagc gccaataccc tggattttca ttatggtaaa
      301 caccataagg cttacgtgga caacttaaac aagctcattg cgggaacgga tcttgatcaa
      361 aaatcgctgg aagaaatcat caacattgcc gctaaagatt ccgcaaaggc cggcattttc
      421 aataatgccg ctcaggtctg gaatcacacc ttctactgga aatgtctgaa gaaaacgggg
      481 ggcggccgtc ccagcggcgc catcgcggct aaaattaatg aaatgtgggg gaattacgat
      541 aaatttgttg aagagctgaa aaatgcaggc gccacacagt tcggcagcgg atgggtctgg
      601 cttgtttcgg acaaaacccg gttgcagata acgaaaaccg ccaatgccga cacgcccctt
      661 gcgcatggtc agaaagctct tttgaccatc gatgtctggg aacatgctta ctatctggat
      721 taccagaata ggcggccgga ttatctttcc gccgtgattc agaatctgat taactgggat
      781 ttcgttaatg ccaacctata ggcagggcgt cttcaatcgt taaaacaata aaaggaggct
      841 ttaaaaatgc ctcctttttt ataggataaa aacagacagg aatgattatt tctttttatc
      901 gactctcact ttaagctctt tcccgacttt aaagaagggc aattttttag gttcgacctg
      961 aattttcgat ccggttttcg gatttcttcc ggtatatgat ccgtattttt tcacaacaaa
     1021 actgccaaat cctctgattt caatgcggcc gtttttcttt aactcatcgg taaaaccctt
     1081 aaaaatgaga ttcacgacat caattgcctt tttttccgtc agatttaact tcgtgcttaa
     1141 agcttcaata agatcggatt tgttcatatc tcctccttaa actgatggca actcatacta
     1201 gccagatgac actgtatttt gatattctct atgcgtgtgt ttattattta ttttttggcg
     1261 aaagtcaagg aaattttgct atttggactt gactgcactg tccagcaatt gactgatttc
     1321 cttccgggtc agatggcgcc actcgccctc tttcaaggca cccaacgcaa gatcaccgat
     1381 cgcttcgcgc acaagcaggg cgactctgta accgattttt tcaaaccctc tccggataat
     1441 tctgtttttc ccttctttga gcgtaagacg caaccagcag ctcctgtcgt tcattttatc
     1501 cactttgaga ttttccggtt tgaaaaaatc atcgtcaacg ttaatgcccc tgctcagttg
     1561 cttgacgtct tgtctggata aatgtccctg gattttgact ttgtaaattt tgggtttctg
     1621 aaaacggggg tgctgaattt gctgggcaaa atcgccgtca ttcgtcagaa gcaacagacc
     1681 tttggaatcg taatccaacc ttcccaccgg ataaaccctc tccggcacac tgcctagcaa
     1741 atccacaacc gtgggcctgt tctgaggatc atgaagggtt gttacaaatc cggccggttt
     1801 atttaaaaca atataatatt ttactttttc cgtggagatg atcttgccgt caacgcgaat
     1861 aatatctttc tgagcatccg ctttttctcc cagtttcgtt acgacaacac cgttgacgct
     1921 gacccgtccc cgggtgatta actcttcagc gtgacgccgc gaggtaatgc cggccgctgc
     1981 aataactttc tgcaagcgct ctttcataaa tcagggattt cttctatctt ttcgtctatc
     2041 ttttcttctg cattttcatc gtcatgctgt tttgcgacag cctcttcttn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt ctatcttttc ttctgcattt tcatcgtcat
     2221 gctgttttgc gacagcctct tcttctatga tggtttcctg ttcggactcg ctgctttcct
     2281 gatccggatc aagaagttcc tgctgatgat gaagttcctt taattcgcga atattgggca
     2341 aatccataat gtctttgagg ttgaagacct ccagaaattt ccgggttgtc ccgtaaataa
     2401 tcggtttgcc gggcacatct ttacggccca caatacgaat aagtttcttc tccagtagac
     2461 ctttaagcgg accgcccaca tccactccgc gtatgtcttc aatttccgat tttacaatcg
     2521 gttgcttata agcgatgatg gccagggtct ctaacgcctg cggggacaga gactgcggtt
     2581 tggcggtttt gagcttcttg atccacaggc tgagatcggg attggtgcgg aattgaaaac
     2641 cgcccgcaac ttcctgcagg agaattcccc cctgacggtc atggtattcc gagaccagtt
     2701 gatcaatgat ttcccgtatc tctgtttttt ccacctccgg aaaaacggcg cgaattcttt
     2761 cgggtgacaa cggggtgtcg gaagcaaaaa tcagagcttc gatgacggct ttaatctttt
     2821 ccatttacga ctccacagcc ggaaatatcc ggataacacc aaacattgaa gtttgatagg
     2881 ctttaatcat tctgagtttg atcaattcca ggatggctaa aaatgtatag ataatgcgac
     2941 ggcggtcttt tttcccctta agcagatctt caaacgtgat gtttttggtc accgtcaact
     3001 gttccatgat ttcgttgatg atatcggtca gcgacagttt ctccagatca atttccatca
     3061 attctttctt atccatctga gagataatac gatggaaggc ctcgatcagt tcaaaaacgc
     3121 tgacctcaat aaactcttcc tcttcatccg ttgatttgat ctccacttca gggagagctg
     3181 ccgcacgggt gaagacatct ctttccagaa gaggccggtt tcccagactg accgcaatct
     3241 ccttgaaggt tttatgttcc aggagctgtc tgaccagttc ggcacgcgga tcttcctctt
     3301 cctcttcggg ttcttcaggc ggcggtaaaa gcgttctgga tttgatatga ataagcgtgg
     3361 aggccatcac cagatattct ccggccaagt ccagattcag cgctttgatc attttcaggt
     3421 actggaaata ctgctcggta atcagcgcaa tggggatgtt gtaaatgtca atttcatttt
     3481 ttttgatcag ataaagcaac agatccagcg gaccttcaaa gatatccagc ttgatggcat
     3541 aattcaaacg ttgctcattg tccataggat ccatgggatg acctaaatta ttattatcca
     3601 tgacggaaat tcaaatcttc attgcggccc ggacttcact catcgtctga cgggcgattt
     3661 ttgccgcttt tcgattgcct tcttcaataa ctccgttgat ttcatccatg tgacttacat
     3721 aataatccat gcgctcgtga acgggttgca gattttcggc aacgcgctgg gccagcattt
     3781 tcttgcagtc cgtacaccct atccccgctc tgcggcattc gacatcgatc tctttcacct
     3841 tctccggtga agaatataat ccatggaaag taaaaacatt gcatatgtca ggattacccg
     3901 gatctttttt tctcatgcgc tgaggatcgg taaacatgga gctgatttta tgctgcagat
     3961 ttttaccgcg atcgctgatg taaatggagt tgtcgtagga tttgctcatc tttctgccgt
     4021 cggtccccag cagcttcgga acattcgtta aaatcggttc cgggacagga aatatttccc
     4081 ggtaaaggaa attaaaccgg cgtgctattt cccgggtcaa ttcgacatgg ggaagctgat
     4141 caacgcctac cggaacgcca taggctttgt acatgataat gtcggcagcc tgcagcaccg
     4201 gataacccag gaaaccgaac gtcgataaat ccttattggc cagctcggtt ttcatttcct
     4261 tgtaggtggg attgcgttcc agccaggcca gcggtgtaat catagaaagc agcagaaaaa
     4321 gctccgcgtg ttctttgacc gcggactgca gaaacagagt gctcttttga ggatcaaggc
     4381 cgacactgag ccagtcgatg accatgttga tgacgttgtc ttgaatgcct tccgtggagc
     4441 tgtattcgct ggtgatcgca tgccagtcgg ccacgaaata aaaacagtcg tattggccgc
     4501 tttcctgcaa tgcaatccaa ttgcgcaatg cgccgtgaag gtttcccaga tgcagctttc
     4561 cggtgggcct catcccgctg agtattctct tacgctccaa aaacaactcc tttttcatat
     4621 gttactcggc atcacaggcc gtcgattcct gtttcgcagt actgtcccgc cgagcgtcgc
     4681 tcgcgggata gttttccacg gcttatggct gtggaatcat tcgaccagca agcttccgtt
     4741 aacgacgttt gtgaaggttg ggtctcatgg caactgacaa atttcaatgc ttcgttttta
     4801 aaaattcggg tctcactaac gaactaaggc ctccggctat gacagaatac ggaggcctta
     4861 gttttaaaga accaattgat ttgttattta actttaccgg ccaattcttt gcctgccttg
     4921 aacttaacga ctttcttcgc gggaatcttg atcgttttgc cggtctgagg attcctgccc
     4981 tctctggctt ttcttttaac caccgagaac gtgccaaagc cgaccagagc cagtttgccg
     5041 cttttcttga gctctttcgt taccgcatcc tggaaagtat ccagagcctt gcctgcttta
     5101 tccttggtaa tgcctgctga tttcgctata accccgatta attctgcctt cgtcatcttc
     5161 taaaatcccc ctttcgtaaa atcaaatttt tgaattttat tatcaaccgc tttcgcggcc
     5221 ataaccaaaa ccatttgaaa cagatcagtc gctctggcaa acatcacgaa aaatgcttcg
     5281 ttattcttta ttctgcaaac taacgttact caaaccggct tgttctctgg tttagaaagg
     5341 agccatcccc ctcaagctcg cgctacgact agcacaataa agattaacaa tcaagaaaaa
     5401 aatgtttttt taatgctttt tcccgtccgt gtgtgctgcg gcgatggcat cctgatcgtc
     5461 gtgctgcctg caccgccagt gtcccatgga agtcggcaat cggctgccat gctttacaaa
     5521 aggtacctgt tatgttaaga cctgcacctt attttttgcg ggggtctatg cagagcgtcc
     5581 atttcgaaaa aataaagtcc tggttttcac tctcccgtcc ggcgtttcac accgtcggta
     5641 tcctcccgtt tattctggga acgattctgg catataagat caaccttgtc ttcgacccgg
     5701 ctgtgtttgc gctgggtgtt tcggcggtta tcctgatcat gctttctacc tatcacatgg
     5761 gagaatactt tgattaccgg gaagatgaaa tatctcagcg gttgtataaa agcaggtttg
     5821 caggcggatc aggcgtcgtt ccggcaaaaa cagtgaaacg ttcggtggct ttacggaccg
     5881 gattgatctc ctttattatc gccgggatca tcggactgac tcttcaattt atcctgaaga
     5941 caggccccta taccctgctt ttaggctgtc tgggcgcttt tccagggttc ttctattcca
     6001 cccaaccggt caggctggtg cagaaaggcg ttggggaaat ttttatcggt ttctgttacg
     6061 gatggcttcc ggttgcctct gcctattata tacaaaccgg cttcgttcat tcggtcattc
     6121 actggatcac cgtccccatc ggcctgacga tcttcaacgt gatcttcctc aacgagtacc
     6181 cggattacga agcggacaag gccaccggca agaaaaacct tctttatcgc ctggggaaac
     6241 gcaaggggaa agtgctcttc ttcgtttttt ctctcctggc atcggcggcc gtgtttgcct
     6301 ctcccgtttt cggagtgccg ttcaaagcgg tttatttcta tctgcctgtg gcgattgtat
     6361 ctttatttat tgccgtaatg atgttacgtg atcaatacga aaacagaaaa accatggagc
     6421 tattgtgcgg tctgaatata gcggttaacc tgggaacttc tctggtttat attctggcat
     6481 atatttaagc gaaatgacga acgttaaaaa gaaggtcaaa gcattttata atcctccctc
     6541 gttactgctc aagcaactgg gcagttctct gttgatggct tatctcaata attggagaaa
     6601 ctatcctcgc tggggctttc aatacggctt ccggtccatc ccggtggcca acggcgcctt
     6661 ggggatgggc tgcatcggtt atccgggaca tcccgtctgg gaagtcaccg gcgcgtgtaa
     6721 tctgaaatgt attcattgtc atgcggtaag cgataaggcc ggccaagatg aattgtccac
     6781 cgacgaaggc aaaagactga ttgacagcat tgcctctgta agtgcattcc gcaccctggt
     6841 ttatacaggc ggcgaaccgc tggtgagacc cgatattttc gagctgctcc ggcactcgca
     6901 gcaggccggt ctcgccaaca tcatcgccac gaacggcacg ctgatcgacg aagaaatggc
     6961 ttttaaactc agagatcacg gtgtggtctg tcatgcgatc agcctggatg ctgctacccc
     7021 tgccattcac gacatggtcc gcaataaacc cggtgccttc gaccggacgc ttcgcgctat
     7081 taaagccaca aagaaggccg gcatcctttt gcagatcaac acgacggcta tggaatacaa
     7141 tatgccgcac ctttccgaac tgatcgattt tgcagaccgt tgcggtgccg gcatcatgct
     7201 gatgtaccaa ctggtcgccg tgggccgggg tgaaaaaatc gaaaaagcca cgttaaagaa
     7261 aagtgccaac aaacatttaa gtgaaatgat cgctgaaaaa cagaaaaacg caacaaccat
     7321 tattgaaccg gttgccggtc ctcagtattg gccgtacctg ctggaaaaaa agggattcac
     7381 cgaaggactc cggctgaaac ttgccggtaa agtgtttcac ggttgcgcag ccggacgcgg
     7441 ttttgtgtac gtcaaagcca acggcgatgt ctggccctgt cctttcgtag aggttaacgc
     7501 cggcaatatc cgtgacagga attttaaggc catttacgaa gagtcgcccg tatttcagaa
     7561 tttgcggcgc cgggaagagc ttttaaaagg cgcctgcggc gaatgccatt acaaaaccgt
     7621 ctgcggcggc tgccgaggac gggcgcatgc ctacagcgga gattatctgc aagaagatcc
     7681 ccgatgttat atccgggaga caacccagaa tcattcgtag cttttctcaa ggatcgccat
     7741 gaatccggac gaaacaagaa gcttccgcct ttgcatcgtg caagcccctc cctgacgtgg
     7801 aacagctcta gcggtcatgc gctggaaaaa cggagttatt atttcttcag cagcaagtag
     7861 agttcgcctc tttccttcag cgtgccggcc gtcttttccg ctttttcacc gaatccgcaa
     7921 aacaaatcca ccctgccggg tcccttgatg gcggcgcctt tgtcctggct gagcacaaag
     7981 cgggaaaatt cgaccctctc cttgacatgt ccatcggtat cgaaaacagg cttgcgcaaa
     8041 cggatgaagg ccagggctcc ttccgggaaa tattccggat cggtcgcaat cgttcggtcc
     8101 ggcgttaccg cttcgcccag agatccaacg ggatcttttt ccagaaagcg aaaaaatgta
     8161 taccgttcgt tatggcttaa agcctcataa atttcttcgt cacgtttccc ccgtaaaaaa
     8221 tcatgacggt acgtgacttc atgaggctgt atcgtgcctt tgtccagcag gaacttcgta
     8281 atgctgcgga aagaccggcc gttggtattg gcaaaaccga ccgtcagaag gtccccgttt
     8341 tccagtttga ttttgccgga cccctggatg tgcagagaaa ataaatccac cggatccgaa
     8401 acccagagga gttcaaggtt tttgccccgg agaacaccgt cgacatcaat ttcccggcgg
     8461 ctgtaatagg gaacaaattt gtcgttctgc atccgtccga tccgggttcc ctttctcacc
     8521 agatcaggcg gtacccgata cagcggatat ttgtatttct ctgttttttg cagagaccct
     8581 tccaggagcg gttcgtaata accggtaaaa agcacacggc tttcatcatc cgtcccggtg
     8641 acctgataaa catcgaatgc ttcggccatc ctttttccca atgtctccgg atccttcgca
     8701 tcgcctaaca gggcgcgaaa agcgagaagc gtttccttga actgctgcgc gtcaatctgc
     8761 ctgtcttcga tctgataaac gttattgcgt ccgcggccct cataaaaacg gatgctgcgg
     8821 tcaatcgctg tttctagcga tgcaggctcc aaatcatcac tgaaagcaac ctgcgtggca
     8881 tccacaggaa tcagcgagtc cagcgttttg aaagacggtt gggaaacggc acgcatgcaa
     8941 ccgccgagaa agaaagccat caagattagc agcaggattt tcagtgaata tctcatagtt
     9001 ccacaatcat cgccacttca tcacagtaat ccgggtattt ggaacagcgg aagcaatccg
     9061 accagatttt ctccggcagc aggttccggt cgatttcttt aaatccaagc ttgagaaaaa
     9121 aatctttttt atacgtcagc gtaaagacct tgaagagccc cagcgtaatg gcctcggaga
     9181 tgcaggcctc cacaagcacc cggccgacgc cttttccccg gtgggactcc tcaacataca
     9241 gcgaccggat ttccgccagg tttgcccaga taatattcat cgcgcatatg ccgataattc
     9301 gggattcatc ctcatcataa tagacgaaaa aatcccgcag agaaccatag atgtccatca
     9361 gagaacgcgg cagcatctcc cctttgcccg acgataaatt gatcaggcgg tgaatgattt
     9421 tgacgtcatc gatgcgcgct tttctcagca tgtgtctcct cccgatactt tgaggttatg
     9481 tgagccggcg ttttggagca tttcgcgggc atgctcccgc gttgtcggtg tcacatcgac
     9541 gccgcccagc attctgctga tttcttctat tttctgctcg ctgtcaagct cgtttacaaa
     9601 agtgagcgtt cggcccttca ccactttttt ggaaacgaac atgtgtttat ctccaaagca
     9661 ggcgatctgc ggcaaatgcg taatgcagat gacctgatga ttggcggaca cagctctcaa
     9721 ttttcttccg acaatttccg ccaccgcacc gccaatgccg ttgtccactt cgtcgaaaac
     9781 catggacaat accgacccgg tgccggagag aacatttttc agggccagaa taattctgga
     9841 caattcccct cccgaagcca ccttatttag cggtctggcg tcttcgcctt tgtttgccga
     9901 aagatcaaat tcgatatcgt cggctcccct aggcccgaag aaatccgtgt tgtcgtcggc
     9961 acggttttta aaatgaacaa aaaaagaggc gtgcggcata ttcaaagcgc gtatttcttt
    10021 cccgactttg tcctccagcg tcttcgccgc tttctttctg ctttcggaca gattccgcgc
    10081 gctctgccgg agtctgcttt tcaccgcttc ttcttcccgg atcattttct gcaactcttc
    10141 atccagcgag gagaccttcc ggagggcttc ctccatgtcc tgtttttttt gaaaaacggc
    10201 ttccagggaa ccgccgtatt ttcttttcaa acgatggatc agatcaagcc gctcctcgat
    10261 ggcggcaagc cgttcggcat cgaaaaaaag gcccttgccg taatcgcgca gaagcagagc
    10321 cgcttcctgc agcgcgacaa aattattttc catctcggct tcggagattt ttagaccggc
    10381 gtctattttt tttatttctt taatctgacg ctgaacttct tttaacatga cgatgacgga
    10441 gtttttgtcg ccgtaaagca gatcaaaggc attacccgcc cattcggaaa gcctctggat
    10501 gttgaccaga accttttttt catccgacag taccgtatcc tcgttcggtt gcggattaat
    10561 atcctgaatt tccttcaact gaaattcata caactccgtt cttgcgtctg cgcttttgct
    10621 caactcctct agtttttcaa ttccagaccg gatctgctga tactgattga aatgttccgt
    10681 gtagaccgcg cgctcagaca ggcagccgcc aaactcatcc agaatatcaa tatgattttc
    10741 agcagccaga atgatctgat gttcatgctg cccgcaaata ttgatcaacc gttcgctgac
    10801 ggctgccaaa ttggccaggg ttgccgcctg tccgttgata aaaactttat ttttgcccgt
    10861 atgggagatt acccggcgga taatcaactc atccgaaaca ggaaaaccca tggcgtcgat
    10921 tttgtcccgc aggggcttat cggctgaaat atcaaaaacc gcttcgacgg ttgcggtatc
    10981 cgcctgcgtt ctgatcatct ccgacgttgc tctgtctccc agaagcagac tcaccgcgcc
    11041 gatgatgatg gattttcccg cgcccgtttc tccggagaga acgttcagtc cttcaccgaa
    11101 cgtaacatgc agttcgtcga taatggcaaa atttttaatg ctcaactcgt taagcatttt
    11161 ttcttttatg cgctcccgaa ggtaaatccc cccagccgag cttggaacgc aggatctctc
    11221 cgtagctgcg gtatggggaa agaatcagca tcgtggcatg ccttgatttt ttcacgatca
    11281 cgacatcgcc ggattttatt ttatgcgttt cctgaccgtc cagcgacagc attgcgcctt
    11341 tgtctttgga ccagagggta atccgaagat gagcatcctc tgaaaaaata atcggtcggt
    11401 ttgtcagggt atgggggcaa atgggattga tcaggatcaa atcttttccg ggaaatacga
    11461 tgggacctcc ggccgacaac gaataagcgg ttgacccggt aggcgtggaa acgatcaagc
    11521 cgtctccctt gtaagttgtc agatattccc cgtccacttc cacttccagt tcattcattc
    11581 tggataaatc gccgcggttc atgacaacat catttaaaac actgccaatg ccggccacgg
    11641 ttttcccgtg ttgtacactg acatcaagca tcattctctt ttcggtcaca aattttccct
    11701 tgagaatcaa ttccatggta gaatggattt catttaagtt cacttccgta aggtagccga
    11761 ggccgcccat attgatgccc agaataggaa cccggtgaac agccagataa cgcgccgagc
    11821 gcagtatcgt accgtcgccg cccagaacca ccgccacttc cgctttcctt gccagttcac
    11881 tccagctgta tccgccgtcc gaaagaattt tcccggaaat ctccgtctcc agaaacactt
    11941 ttgcgcctcg ttcttccagc cactttttca aagatcggac gtggtctgca attttttctt
    12001 tttcaatatt ggcggcaatg ccgatttttt tgattttcat tgatttcacc cttgaaatat
    12061 gaaggtccct aacacaaaaa aaaacctctg tccacggata aatagtctgc ggatgcattt
    12121 ataaactgat tgatatttaa gagaaatccg gagtcattac ttcttgacac atccggcccg
    12181 gataatatag gctgcggcaa aaatggggat tgaaaaatgg gtttactcaa gggtggtttt
    12241 accttctcgc gtttcagcgt cgatggccag ttaccgcagg cctttttaaa ttttgtcaac
    12301 aaccggatcc gggccaattc ttttaaaaat tcattcaagt cgacggaaga aaaaaccatg
    12361 ggatgggttt ctttaaccga catactggat accgatttta agaatgccaa ttattcactg
    12421 ggcgcctacc tgattttttc cttgcgcatt gaccgcaagc tcatcccgcc gaagctcttg
    12481 aagatcaaac tgatggaaga ggaaaggcgt tttcttgccg aaagcggcaa gaataaaata
    12541 ttccggcaac aatcaacggc tctgaaagac aaggttaaac ttgatctgct ggccaaactc
    12601 gacgccatcc ctttttttta cgatgtttgc tgggcggtgg aacaaaatac actttatttt
    12661 tcttcacttt ccgacaaggt tatcgatgat ttcgcggatt tatttaaaaa aacgtttgca
    12721 ttgaatctga aacgcttttc cccgcaggaa aatcacctga tcagaaagga tgcggatcca
    12781 gccgaatcga cgtccttgat cggccgcgag tttctcacct ggctgtggtt taaatcggaa
    12841 gaacgtaacg gcagaatcgt gctgccgaac aaagaggaat gtgaaattca ttttctgaaa
    12901 agagtttcgc tggaagccgg cgaaggcgaa tactctcagg gtgtcgtctg tcacggcatt
    12961 cacgcagaac tcaaggaagg caaggaagca atccgccagg ggaagaaagt caaggaagcg
    13021 ggaataaaat tgcttcataa caataacgaa tgggatttta tgttcaaagc cgattcgttt
    13081 gatttccagt cgctgaaaat gccgcaaacc gactggcagg atacgccgga agacccgtca
    13141 ggcagcctgc tggagagaat ctatctgatc gaaaatgccg taaaaaccat cgataccatc
    13201 tatgaatctt ttcttcacat caggcaatcg ccgcagtgga aggaaaaaga actcaaactg
    13261 cttcacaagt ggctggaaaa aaatcccaaa gattaaatcg ttatttatct tctctttttt
    13321 tatcctcgcc ggataaacgc gtgatgatcc gaccgggaat ttcatccagc ggtaactcat
    13381 cacaggccgc tcccagttta accgcctctc cgggcatccc gtaaacgaca gacgtggctt
    13441 catcctgggc aatggtatat gctttatttt gccgcatggc cagcaggccg tccgcgccat
    13501 cggctcccat acccgtcaaa agaacaccca tcgcattggc tccagcgtgt ttggccaccg
    13561 aatgaaaaag aacatcgacg cttggtttct gatgatgaac catcgggcct cccttgattc
    13621 tgacctgata aaatgctccg ctgcgctcca gaagcatatg atatccgccg ggagcaatca
    13681 gagccagtcc gggagccgcc gtatcgccgt ctttagcttc gcgcacttcg attttacaca
    13741 acgcattcag tcgctcggca aaactggccg tgaaatacgc cggcatgtgc tggaccacga
    13801 cgattccggg aatattcatg ggcagccggc tgaggatttt ttcaatggca atggtaccgc
    13861 ctgtggatgc ccctatggca atgaccttat gggtcgtctt gagttgaatt ttatatcccg
    13921 gaacgctttc tctttccacc aattcaacag ccggtttggc aattttggcc cgtgctgccg
    13981 cgcgtatagc ttccaccagg cgtacggaaa gatctgcagt ggacaaactc gatcccggtt
    14041 tacagatgac ttccacagct ccgagttcca gggctcgtat cgcgttctga ctgtcgcggg
    14101 gggtcagaga actgaccacc acaaccggca tcggatagaa tttcatcagc ttggacagaa
    14161 aggaaaggcc gtccattctc ggcatttcaa tatccagggt aatcacgtcg ggcttaagac
    14221 ggacaatctt gtcacgcgca acataaggat caatggcgcc tcccaccact tcgatgtctt
    14281 tatggcggga caactcttcc gtcaatactt tgcgaacaag cgccgaatca tcaacaacca
    14341 gaactttgat caatcgttta tctcctggtc cgaaatgtag tcatccacaa ataatttgat
    14401 atctgcccgc ccctgattaa aatttaaaat cacgacggcc gaaggtttac tcttttcctc
    14461 tttcagcaat tcatccctgc cgagatgtct ggggctttga atcttgtatc ctcccgcttt
    14521 tcttcgaccg agcgacggca caatgtttcc gcagatgata ttggcaatct cgccgagggc
    14581 atcttttttc tcctggaggc ttggatgatc gctacttaaa atattcgaag caatggctga
    14641 aaacaagccc cctctgcttg cgatcaccaa acgtccgttg taagcgccat gaaattcaac
    14701 ctcagcagca gcatccggct ccagagcagc ctggctgtct ttgagttccg gttcaaggta
    14761 cataaaacag atatcttcaa acgttttaat cgtggcttct tccagaatct tttcaatctt
    14821 cgttttcatg gctaaccccc gtaacttcaa cgataacttc tttaagaacc tccggtgtaa
    14881 acggtttatg gataaatgta gcgcccaatt ccagcatttt ttctatttta cccggatcgc
    14941 tttccgaact gatgacgatg accggaaggt tcttatactg atcattttct ctgactttta
    15001 tcagcatttc ctcaccgtcc attaccggca tgtgaatatc gatcatcgcc aaatccaccc
    15061 agtgatcctg taaaacgtgc agtccttctt ctccgttttt cgcttcccag atatcaccca
    15121 aaggcagccc cgccagtttc aatgttttta tgatgatagt tcgcataaca gagctgtcat
    15181 caaccaccag agtatttaag gccatttcac tctctcctgt ctaatctctt tagcatttca
    15241 tttctcattc caagaaatcc ttaaagttgt ttgaggaaag tttgagactt aatcagcatc
    15301 tttccgcttt ccaattccag cgtgacggta cgcgaaccgt aaccgcccac gtcataagct
    15361 ttcagcatga gaccttcatt ccataaatat ttccgtaaac tgacaaaatt gcggcggcca
    15421 atctggaaaa aatcctcatt ttcgttgagt tttgaacttg atcccccggc tacccggatg
    15481 atcagatcgc tttttcgtga acctttctga taaaatttat ccatcatggc tttcattccg
    15541 ctgtccacgt ataaataagg attgcctgct gccttgtttt ggtcgatccc ggaatccggc
    15601 atcatacaat gcaacaaacc gcccgtcttt tttctgaaat cgtaaaccgt cacccccaga
    15661 cagctgccca gcgcatatgt aatcaacata tcggcactct cgtcggatat cttcaggtcg
    15721 gcaactccaa ctgtatagac catattactt cacgtccgtt ctaacctttc cggttttttt
    15781 gacgaccgaa tgcgccttcc tgatcttttg ataaacggcc ggcctcatgt aacgatactc
    15841 gtgttggagg aagctcaggc tttccgaatg tccgaccatg agatacccat cttcttttaa
    15901 aagatgccag aacctgccca cgagtttttc ctgtgtcgat ttgtcgaagt aaatcatcac
    15961 gttgcggcag aaaataacgt caaaaggccc cttcatcggc cagtgttcca tcagattgag
    16021 cttggcaaaa ctgaccatcg actgtatttt gggaatcact ttgtatttat aacctgatcc
    16081 cgtatctgtt ttttgaaaat acttgtgttt caagtgacgc gacattattg cgagactttc
    16141 ctcatcataa agaccctgcc gggctttttc catcatccta tccgagatat ccgtcgccag
    16201 tattttaata tcgagtgtgg cggtatcagg aatggattcc aataaaacga tggcaatcgt
    16261 gtacggttct tcacccgaag aacatccggc actccagatt ctgaccggac ccttaaccca
    16321 accgggaata atttcgtgtt tcaaaaaatc aaggtgctct gattccctga aaaaattcgt
    16381 cttgttggtg gtgaggatgt ccaccatcgc cccgatttcc gttccgctct tatcgttggc
    16441 aatatgcttc aggtactgat taaaatcatg aaatttcaag tgcctgagcc gtttgataag
    16501 ccgggccttg accagttctt ccttgccttc gtgaagatcg attccggaaa tctgataaac
    16561 cagtctgctg attttatcaa attgctcgga agtaagtttt agagaataat aatccgtcga
    16621 aagattattc cattccgaat gcataatatc ttttcttttg tgttggcaga tttctacttg
    16681 tccgaaaaaa atttcataac gaccggtacg aactagaaat tctgcaatat atcatgatcc
    16741 ttatcatcca gcggtatgac cttgcgggga tcaggatgaa gaccggctga cgctttatca
    16801 ccttttttcc ctggagcgac atccggcaga cgatattccg tttttttaat ggcatccgga
    16861 ccggccgtta atgcaacggt cttcgtcagt ctgaatccgg cgaccatggc ccgcatttcc
    16921 tcggactgcg aagacatctc ctcggcgg
//
LOCUS       DAOOLB010000040        16916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_3555_length_16916_cov_42.377558, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000040 DAOOLB010000000
VERSION     DAOOLB010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 16916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16916
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3555_length_16916_cov_42.377558"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="PLF77_06515"
     CDS             complement(<1..878)
                     /locus_tag="PLF77_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08542.1"
                     /translation="MKKIVVCVVVMMFLVTLPFAALAKKVSLTEEELSSTTAQEGVTI
                     QFGKNTYGGDYFFDTNFYVAGKFLPTIQSWGDGDGCTDCGGYTEKGWIGVRNVSMDPF
                     VSLGLPNPAFPPTGSFISLYRTMKIDVGTSGGATKLFIGMPSIMVHPSGISQTLSSGT
                     TETLGQDFGTAYMSEFALIANSAGTGYLRIGNHSSTGQQGVEIQFSSTSPVAAGPLYG
                     VVFSIPGKPIVQSWSDIDGEQTGSGYTNAGYFGAKNFTMSDGTTYAWPGSPSTFNMIV
                     TGTLSIDVGTNGSGQTA"
     gene            complement(1206..2099)
                     /gene="htpX"
                     /locus_tag="PLF77_06520"
     CDS             complement(1206..2099)
                     /gene="htpX"
                     /locus_tag="PLF77_06520"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF003965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HPC08543.1"
                     /translation="MKRILLFLATNIAVLVVLSIVLRLLGVDRILDESGMNLNMVNLL
                     VFAAVFGFGGAFISLAMSKWMAKRMMGAQVITNPQSATETWLVDTVKKHAQMANIGMP
                     EVAIFNSPSPNAFATGMNKNKALVAVSAGLLHAMNKEEVEAVIGHEISHVANGDMITL
                     SLIQGVVNTFVIFLSRIVGHFVDRVILKNEEEGHGIGFFVTSLVAQIVFGILASVIVM
                     WFSRKREFIADAGGAKLAGTQNMINALEALKRGTAEPLPDQMAAFGINGKPSGHGLKL
                     LFMSHPPLEDRIEALKKLTYR"
     gene            2425..4260
                     /locus_tag="PLF77_06525"
     CDS             2425..4260
                     /locus_tag="PLF77_06525"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HPC08544.1"
                     /translation="MKKNDVVQYPQTISGFEFFVKFMAFVGRIPFVVYHGIKGIKSIS
                     SNRNASWGSLLQKSALKYPNNPAVKSPDGTLTYAELNEKANQFAHFLLSKNVKKGDTV
                     AVCLENRPELLFLYCGCAKVGAVCSLINTNQRGDSLVHSFNLNKGKVMVVGEECYSFF
                     KDVKDRIDLQGSELCLLRDPAMNQLKAPEDITDQLAVLPKTNLSETGGISLKDTMAYV
                     YTSGTTGGLPKAAVITHKRAMSATFWFGRVVTHVKPKHTIYIPLPFFHTNAITVGWPP
                     ALYSGAAVAMRRKFSASGFLDDVKKFQATHFVYVGEVCRYLMAQPEKAGENKTTLKYI
                     IGNGLRPDIWMAFKRRFGISKIYEFYGAAEGVGVFTNLMNFDYTVGTSLTPFAIVQYD
                     IENDCPARDAGGFLIKVAKGDPGLLIMEISEKTPFPGYTDKKKTEEKIIRNAFRKGDL
                     WFNTGDMLRNMGFRHAQFVDRLGDTFRWKGENVATMEVEKALDSFSGVSASAVYGVVM
                     PKGDGRAGMAAVIKQEGKDVDFAALAEHLRSVLPKYAVPIFIRFVNDFQWTATHKIKK
                     NDLKNDGYNLSAIKDGLYVLLPGTETYVPLNDAIYQDIMAGKYKF"
     gene            4507..7296
                     /gene="ileS"
                     /locus_tag="PLF77_06530"
     CDS             4507..7296
                     /gene="ileS"
                     /locus_tag="PLF77_06530"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPC08545.1"
                     /translation="MDYKKTLNLPQTDFPMKANLAQREPEMLKKWEDMNIYKKIRQMA
                     KGKTPYILHDGPPYANGNIHLGTALNKIIKDIVIKSKNMTGFDGVYVPGWDCHGLPIE
                     HQVDKELGAKKGAMSQVEKRRACRTYAEKYVDIQREQFKRFGVFGEWENPYLTMAYPY
                     EAVTVEEFGKLYLNGSVYKGKKPVYWCATCRTALAEAEVEYADHHTPSIYVKFNFVSD
                     IAQVLPKLAAQKVAMVIWTTTPWTIPANLAIAVKDDFIYVAVKINDDVMIVAKDMLDY
                     CLDAFGYQGKSYEIIDEFKGAVLEGQKCVHPLIKRNSLLILAPFVTLDAGTGAVHIAP
                     GHGQEDYEIGLEYGLDNYAPVDDAGKFTEDVEHFAGQFVFDANDSVIKKLDEVGALLG
                     HVPMQHSYPHCWRCKKPIIFRSTEQWFISMEKNDLRKKALECINEVTWIPSWGKDRIY
                     GMVENRPDWCISRQRLWGVPITVFYCAKCKNEVMTREMMDHLVALVEKNGADVWFEKD
                     PKDLMPKHTTCPHCKGTEFTKEVNILDVWFDSGVSHAAVLEKRPDLSSPCDMYLEGND
                     QHRGWFHSSLLESVGTRGRAPYKNVLTHGFVVDGEGKKMSKSVGNVIGAEEAINKYGA
                     EILRLWVAAEDYTDDIRISEEILKRLMEAYRRIRNTSRFILGNLYDFNMDKDAVAYEQ
                     MSELDRWALHRLQEVIKRVTEAYERYQFHVVFYTLYNYCTVDLSALYLDVLKDRLYTN
                     KAASAVRRSGQTAMFIILNAMTRLLAPILTFTSEEVWAAMPDWTNKEDSVHLAQFPEV
                     SGTFFNADLGERWKAMIDAKSEIAKAVEQARKEKIVGHSLDARITIAAPEKLRALFAA
                     HLEDLKALLIVSQLQLADEQDIAAPFRSAEIEGLMVGVEKARGAKCERCWIYDESVGA
                     DAQHPTICARCLPNL"
     gene            7312..7800
                     /gene="lspA"
                     /locus_tag="PLF77_06535"
     CDS             7312..7800
                     /gene="lspA"
                     /locus_tag="PLF77_06535"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HPC08546.1"
                     /translation="MKKNIIIFVLGAAVIVVLDQITKAAIVKKFFLHETRSVIDGFFN
                     LVYVMNPGAAFGFLAGMSEMFRSLFFIGMTLLVMALIIYYIAKSKSENMFFVVSLTMI
                     FAGAVGNLIDRIRFGAVVDFLDFYIKTAHWPAFNVADSAITIGAVLMIWGMVAGRKRE
                     AV"
     gene            complement(7879..8172)
                     /locus_tag="PLF77_06540"
     CDS             complement(7879..8172)
                     /locus_tag="PLF77_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08547.1"
                     /translation="MSLLIGGIISAIFGLVSLIFFWPDFMTIFKGSLPIFLLLGGALA
                     VYVGIDEIQEKNRQERHLQEEKLEHAQKEIETIRAQAESYREELQKLKDSKQP"
     gene            8336..8710
                     /gene="hisI"
                     /locus_tag="PLF77_06545"
     CDS             8336..8710
                     /gene="hisI"
                     /locus_tag="PLF77_06545"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HPC08548.1"
                     /translation="MIEPDFAKGDGLIPAIVQDAETKDVLMMAYMNRESWDATLKTGK
                     ATYWSRSRKKLWLKGESSGNVQIVKAVFIDCDEDTILLHVQQIGDAACHTGHRSCFYR
                     KLEGNDFVAVGEKIFNPEDVYK"
     gene            8707..9579
                     /gene="hisG"
                     /locus_tag="PLF77_06550"
     CDS             8707..9579
                     /gene="hisG"
                     /locus_tag="PLF77_06550"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HPC08549.1"
                     /translation="MKNLKLGIPKGSLEANTIDLFKKAGWRITSDARSYFPDIDDPEI
                     SCKLVRAQEMSRYVEDGTLDLGLTGIDWIMENDSDVVVVQDLIYSKVSTQQARWVLVV
                     RDDSPYKKIEDMEGKRISTELVNFTKKYFAERNINIHVEFSWGATEAKVVEGLVDAVV
                     EVTETGSTIKANKLRIIHELMKTNTQLIANRAAWKNAWKRKKIEQISTMLTGSLQAMG
                     KVGLKLNVSKENLKGVIGLIPSLKSPTISGLSSDKWFAIESIVDAKVMRDLIPKLLDA
                     GAQGIIEYPLNKVI"
     gene            9594..10181
                     /gene="hisB"
                     /locus_tag="PLF77_06555"
     CDS             9594..10181
                     /gene="hisB"
                     /locus_tag="PLF77_06555"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HPC08550.1"
                     /translation="MARKAKIVRKTTETDIRLEIDLDKTAGSSIETTIPFFNHMLELF
                     ARHGFLKLIVRSKGDTHIDDHHLVEDLGICLGQAVAKALGDKKNINRYGSARVPMDEC
                     LCQVDMDICGRPYLIYHVKSGRRKIGKFDPALVKEFFKAFTDQSGITLHINFLYGENI
                     HHMIESIFKAFGRALRNAVSLNENIKGVLSTKGKL"
     gene            10288..10974
                     /locus_tag="PLF77_06560"
     CDS             10288..10974
                     /locus_tag="PLF77_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08551.1"
                     /translation="MVAILRKYFCFIVRFVFVALLAFPVMSQALDKKAQSDESENLIA
                     VIAFQPLRPEGGTGNTVLCPICGKASAGGIIQEGAEKIVEEIFVEQLKARDDIVLVPA
                     EKLQKATQKYSTDSMKDFQATGLLKIGRELKADFLVVGYVYRFAERVGYRYSSEHPAS
                     VVFEIHLIRTADGKSVWRGYFDKTQRSLMENVLDISSFFKGGAKWMTARELTELGMEN
                     VFQTFPDFQP"
     gene            11127..11864
                     /gene="hisA"
                     /locus_tag="PLF77_06565"
     CDS             11127..11864
                     /gene="hisA"
                     /locus_tag="PLF77_06565"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HPC08552.1"
                     /translation="MIIIPAIDIKDGKCVRLAQGKFDRVTTYADNPLDMALLWARKGA
                     ELIHIVDLDGSVAGRPRNADIILNIVENINVPVQIGGGIRDMETIRFYLDQGVSSVIL
                     GTTALKNQQIVREACTVFPGRIILGIDALNGKVAVRGWTENTEKNAVDLARQYEPYGV
                     KAIVYTDIERDGMGTGINMKATRELADSVSIPVIASGGVASIADVDNLMVVEGGSIFG
                     VIIGRALYTGAVDLEEAISKTKKVTRT"
     gene            11883..12227
                     /locus_tag="PLF77_06570"
     CDS             11883..12227
                     /locus_tag="PLF77_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HPC08553.1"
                     /translation="MSDCVFCKIVRGEIPCARVFEDEHVLAFDDIHPMAPVHVVIIPK
                     KHIATLMDVDVDNSDVIHRLTAAAQKVARIKKIDQSGFRTAINCNAEGGQVVFHLHVH
                     VLGGRKLQDELG"
     gene            12231..12692
                     /locus_tag="PLF77_06575"
     CDS             12231..12692
                     /locus_tag="PLF77_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPC08554.1"
                     /translation="MEGEKMDINAKLSEEMVAAAKAKDKIRLSAARMLKTALHNKEID
                     LMRPLHESEMLQVLSSMIKQRKDSIEQFAKGGRTDLVEKEEAELKFLQEFMPAQMSDE
                     EVDALIKKTIEDAGAVSVKDMGKVMKILMPKLTGVADGKAVGEKVKAFLSQ"
     gene            12740..14512
                     /gene="dnaG"
                     /locus_tag="PLF77_06580"
     CDS             12740..14512
                     /gene="dnaG"
                     /locus_tag="PLF77_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPC08555.1"
                     /translation="MYVDQSKIEEIKNRLDIVDLVSEYLTLKKAGRNFIGLCPFHQEK
                     TPSFTVNRDKQIFYCFGCGEGGNVITFLMKIADKTFPEAIRDLAVKTGVVLPPVSSGK
                     EARQKDLLKENIVSLNSKAAQQFSRNLFSASGTPARQYLQQRGIAEDVVKQFRLGYAP
                     DTWRSLTDFMEKSGLSLKLAEQAGLIIPGKEGGFYDRFRGRLIFPIENVFGEIVAFGG
                     RILGKGEPKYLNSPESPVYIKGKNLYGLNKAKDDIRKKDFALIVEGYFDLISLWNAGI
                     RNVVATLGTALTRDHLDLLRRYCVDVVALFDPDEAGKKALDRSLELFLSTEMRARALI
                     LPDGYDPDDYVRKFGQDKMEDLIALAQPLSDYYIENVIGGGRTFEDKRDMVKTAMTFL
                     SKIGDQKEKNLFIKRIAEKTGLNEELLIREAYSRVYSGRTETNRNNPAVNVHHDTVEL
                     HLIQLMMEYPQKIVQVENENVLDYFLQPDIRKLGEKIIDAYKLLGFVDVGVILSTEEN
                     QALRERIFQLMIKALPAEDDVLGKILTDNIRQIKKRWFKEQHRQIKIKLSRAQDSGNE
                     EMVHQLLYEKEQMLIKEKELQSTG"
     gene            14618..16198
                     /gene="rpoD"
                     /locus_tag="PLF77_06585"
                     /pseudo
     CDS             14618..16198
                     /gene="rpoD"
                     /locus_tag="PLF77_06585"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            16423..16498
                     /locus_tag="PLF77_06590"
     tRNA            16423..16498
                     /locus_tag="PLF77_06590"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16456..16458,aa:Ile,seq:cat)
BASE COUNT         4609 a         3783 c         4470 g         4054 t
ORIGIN      
        1 gccgtttgac cgctgccgtt ggtgccgaca tcaatagaca acgtaccggt aacgatcatg
       61 ttgaaagttg aggggcttcc aggccatgcg tatgtcgttc catccgacat cgtaaagttt
      121 tttgcaccga agtaacccgc gttcgtgtag cccgaacctg tttgctcacc gtcgatatcg
      181 ctccaggact gaacaatggg tttgcccgga attgagaaaa caaccccata cagaggaccg
      241 gcggcaacag gagaagtgct gctgaattga atctccaccc cctgctgtcc tgtggaggag
      301 tggtttccga ttcgcagata gccggtacct gcagaatttg cgatgagggc gaattcactc
      361 atgtaggctg tgccgaaatc ctgtccaagc gtttctgttg tgccggaact taatgtctga
      421 gaaatgcctg acggatggac cataatactt ggcatgccga tgaataattt tgttgcgccg
      481 ccgctggtgc cgacgtcaat cttcattgtc ctgtaaagag aaataaaaga acctgtggga
      541 ggaaaagcag gattaggaag tcctaagctt acgaaaggat ccatgcttac atttctgacg
      601 ccgatccagc ctttttctgt gtagccgccg caatcggtac aaccgtcacc gtctccccat
      661 gattgaatgg tgggaagaaa tttgcccgca acgtagaaat tggtgtcaaa gaaataatct
      721 cctccataag tgtttttccc gaactgaata gtcacacctt cctgagctgt cgtggatgaa
      781 agttcttctt cagtcagaga cactttcttg gccagagcag caaaaggcaa cgttacaagg
      841 aacatcatga cgacaacgca aaccacaatc ttcttcatat tcttctcctt aaaaaatttt
      901 taagagttga cacctaatat tttttaattt ttttctgtaa gatttaaggc tctgaatgtc
      961 taaaacaagc cttgtcggtt ttttacgtta tttgcaagga gttgtcaaga atattttata
     1021 atttatttat aattaaaaca gtaattttca aagaacaatc attgttgttt gaagaagtca
     1081 taggtcaaaa aatttttaca gatcgggttc gtccgcccgg ccggattcaa acaaaaaagc
     1141 cggtgcgttt ttacatgttt ttttacacga aacggaccgg ccttttttga ggatatttta
     1201 ataatttatc ggtatgtcag tttttttaag gcttctatgc gatcctcaag cggcggatgg
     1261 ctcatgaaaa gaagtttgag cccgtggccg gaaggcttgc cgttgatgcc gaaagccgcc
     1321 atctgatcgg gcagcggttc agcggttcct cttttgagcg cttcgagcgc gttgatcatg
     1381 ttttgcgttc cggccagttt ggcgccgccc gcatccgcta taaattcccg ttttctgctg
     1441 aaccacatca cgatcacact ggccaggatg ccgaagacga tttgcgcgac gagggacgtg
     1501 acgaaaaatc cgatgccgtg gccttcttct tcatttttca gaatgacccg gtccacaaaa
     1561 tgacccacaa tgcgggacag gaaaatgacg aaggtgttca ccaccccctg aatcagggac
     1621 agggtgatca tgtcgccgtt ggccacatgg cttatttcat ggccgatgac cgcttcgact
     1681 tcttccttgt tcatcgcgtg aagcagtccc gcgctgacgg ccaccagcgc cttattcttg
     1741 ttcattccgg tggcgaaagc gttcggggac ggagagttga aaatggcgac ttccggcatg
     1801 ccgatattcg ccatctgagc atgttttttc acggtgtcca ccagccaggt ttcagtcgca
     1861 ctttggggat tggtgatgac ttgcgcgccc atcatgcgtt tggccatcca cttggacatg
     1921 gccagcgata taaaagcgcc tccgaaaccg aataccgccg cgaagacgag caggttaacc
     1981 atattaagat tcatgccgct ttcgtctaga atacggtcga caccgagcag acgcaaaaca
     2041 atgctcaaaa cgacaagcac agcgatattg gtcgcgagaa acaacaaaat tcttttcatt
     2101 tttaacctcc tgagttaaaa actgccccgg gcagccattg acggtacgtc ttgacaattc
     2161 tttttgttat tttaatggca agaaccggga tgtcaagtct ttatcgtgaa aaagtcccgg
     2221 attgtttttc tccggaagat gtttttgcgg gattgggcgg aaggaatcgg attatgggcg
     2281 ctttgattgt cgctggtatt gttttttccc caaggggacg gactcaaccg gcaccccgaa
     2341 tttgcttgac acagacaact atcttgtttc aagatatctg cacattcaat cattattcaa
     2401 agcggccaac caggaggaat caaaatgaaa aaaaacgatg tcgtacagta tcctcaaact
     2461 atcagcggtt ttgaattttt tgtcaagttc atggcgttcg ttggacggat tccatttgtc
     2521 gtctatcacg ggatcaaggg gatcaaatcg attagcagca atcgcaatgc ctcctgggga
     2581 tcgcttctgc aaaaatccgc actcaagtat ccgaacaacc cggcggtgaa atcacccgac
     2641 ggcacattga cctatgccga acttaatgag aaggccaacc agtttgccca ttttctgctg
     2701 tcgaaaaacg ttaaaaaagg cgacacggtt gcagtatgtc ttgaaaacag gccggaactg
     2761 ctttttcttt attgcggatg cgcaaaagtg ggggctgtat gctctctcat caatacaaat
     2821 cagagagggg attctctggt acacagtttc aatctcaaca aggggaaggt gatggtggtc
     2881 ggagaggagt gctacagttt cttcaaggac gtgaaggacc gaatcgacct tcagggatcg
     2941 gagctttgtt tgctcaggga tccggcaatg aatcaattaa aggcgcccga agacattacg
     3001 gatcagcttg ccgtcctgcc caaaaccaat ctttccgaaa cgggcgggat cagcctgaaa
     3061 gatacgatgg cctacgtcta tacctcggga acaaccggcg ggttgcccaa agcggcggtc
     3121 atcacccata agcgggctat gtccgcgaca ttctggttcg gaagggtcgt aacgcatgtc
     3181 aagccgaaac acaccatcta cattcccctg ccttttttcc acaccaatgc catcaccgtg
     3241 ggatggccgc cggccctgta cagcggagcg gcagttgcca tgaggcggaa attcagcgcg
     3301 agcggctttc tcgacgacgt gaagaaattc caggccacgc attttgtcta cgtgggagaa
     3361 gtgtgccgct acctgatggc acagcccgaa aaagccggcg aaaacaagac caccctgaaa
     3421 tacattatcg gcaacggcct gagaccggat atctggatgg ctttcaagag gcgtttcggc
     3481 atcagcaaaa tatacgaatt ttacggagcg gccgaagggg tcggcgtgtt taccaatctt
     3541 atgaattttg attataccgt gggaaccagt ctcacgccct ttgccattgt ccaatatgat
     3601 atcgaaaacg attgtccggc gcgcgatgcc gggggcttcc ttatcaaagt cgccaaaggc
     3661 gaccccggcc tcctgattat ggaaatatct gaaaaaaccc cgttccccgg ttataccgac
     3721 aaaaagaaaa ccgaagagaa aattatccgc aatgccttca ggaaaggcga tctctggttc
     3781 aataccggcg acatgcttcg caatatggga tttcgtcacg cgcagtttgt cgatcgcctc
     3841 ggcgatacct tccgctggaa gggcgaaaac gtcgcaacca tggaagtgga aaaagcgctg
     3901 gattcttttt ccggcgtgtc cgcttcggcg gtttatggcg tggttatgcc gaaaggcgac
     3961 ggcagagccg gtatggccgc cgtgataaaa caggaaggga aagatgttga ttttgccgcg
     4021 ctggcagagc acctccggtc cgttctgccc aaatacgcgg tgccgatttt tatccgtttt
     4081 gtcaatgatt tccagtggac ggcaacgcat aagatcaaga agaacgatct gaaaaatgac
     4141 ggatataatc tgtcggcgat caaagacggc ctgtatgttc tcctgcccgg aaccgaaaca
     4201 tacgttcccc tgaatgacgc catctatcag gacatcatgg ccggaaaata taaattttaa
     4261 gagacttgtc tgtagccgtt tccatggcag gtgggttcgc ctgaccatat ggagacattg
     4321 agattgttcg gcttatcggg ttattcaatc tgtctgtgac gcctggatct catgaagttt
     4381 gcttgcaagt gtcggaataa tatggtagtt cacttcaact taaccagggc gcctgcagag
     4441 gttgtcctgg ttttagattg taaaaagatt attcgtgacg tgatcactta aatacaggag
     4501 caaacaatgg attacaaaaa gaccctgaat cttccccaga cggattttcc gatgaaagcg
     4561 aatctggcgc agcgcgagcc ggaaatgctg aaaaaatggg aagacatgaa catctataaa
     4621 aagatcagac aaatggccaa ggggaaaacg ccttacattt tgcatgacgg tcctccttat
     4681 gcaaacggca atattcatct gggcaccgct ctcaataaaa tcataaagga cattgtcatt
     4741 aaatcaaaaa acatgaccgg atttgacggt gtctatgtgc cgggctggga ttgtcacggc
     4801 ctgccgattg agcatcaggt ggataaggaa ctgggcgcca aaaagggggc gatgtcgcag
     4861 gtggaaaagc gccgcgcctg caggacctat gcggaaaaat atgtcgacat tcagcgtgaa
     4921 cagttcaagc gtttcggtgt tttcggcgag tgggagaatc cttatctgac gatggcctat
     4981 ccttacgaag cggtcacggt tgaggaattt gggaagcttt atctcaacgg cagtgtttat
     5041 aaaggaaaaa aaccggttta ctggtgcgcc acctgcagaa ccgcgctggc ggaagccgaa
     5101 gtggaatatg ccgatcatca cacgccgtcc atttacgtga aatttaattt tgtctccgac
     5161 attgcccagg tgctgcccaa actggccgcc cagaaagtgg cgatggtcat ctggaccacg
     5221 acgccctgga cgatcccggc caatctggcg attgccgtca aggatgattt tatttacgtg
     5281 gcggtgaaaa tcaatgacga cgtaatgatt gtcgctaaag acatgttgga ttattgtctg
     5341 gacgcctttg gatatcaggg caagtcctat gaaattatcg atgagtttaa gggcgcggtt
     5401 ctggaaggtc agaaatgcgt tcatccgctg attaagagaa acagtctgtt gatcctggcg
     5461 ccgtttgtca cgctggatgc cggaaccggc gccgtgcata ttgctcccgg ccacggtcag
     5521 gaagactatg aaatcggcct cgagtacggc ctggataatt acgcgccggt ggacgacgcg
     5581 ggaaaattca cggaagatgt cgaacatttt gccggacagt ttgttttcga cgccaacgac
     5641 agcgtcatca agaaactcga tgaagtcggc gcgcttctgg ggcatgtgcc gatgcagcat
     5701 tcctatccgc actgctggcg ctgcaagaaa cccatcattt ttcgttccac ggaacagtgg
     5761 ttcatttcga tggagaaaaa cgatctgcgc aagaaagcgc tggaatgcat taacgaagtc
     5821 acatggattc cctcgtgggg gaaagaccgc atctacggca tggtggaaaa ccgcccggac
     5881 tggtgtatct cgcgccagag attgtggggc gtgccgatta ccgtgttcta ttgcgccaaa
     5941 tgcaaaaatg aagtgatgac cagggaaatg atggatcatc tggtggcgct ggtggaaaaa
     6001 aacggagcgg atgtctggtt tgaaaaagat ccaaaagacc tgatgccgaa gcatacgacc
     6061 tgtccgcact gcaaagggac ggagtttacc aaggaagtga atattctgga tgtctggttc
     6121 gattcgggag tcagccacgc ggccgttctg gaaaaacgtc cggatctgag ttcgccctgc
     6181 gacatgtatc tggaaggcaa cgatcagcat cgcggctggt ttcattcgtc cctgctggaa
     6241 agcgtgggta cgcgcggacg cgcgccgtat aagaatgttc tgacacacgg ctttgtcgtg
     6301 gacggggaag gcaaaaaaat gtccaaatcc gtcggcaacg tcatcggcgc ggaagaggcg
     6361 atcaataaat acggcgcgga aatcctgcgg ctgtgggtgg cggcggaaga ctacaccgac
     6421 gacatccgca tttccgaaga aatcctcaaa cgcctgatgg aagcctatcg ccggatccgc
     6481 aacaccagcc gtttcattct gggcaacctc tacgatttca acatggataa ggatgcggtg
     6541 gcgtacgagc agatgtccga gctggaccgg tgggccctgc atcgtttgca ggaagtcatc
     6601 aaacgcgtga cggaggctta tgaacggtat cagttccacg tggttttcta cacgctttac
     6661 aattattgca ccgttgatct gagcgcgctt tatctcgatg ttttgaagga ccggctctac
     6721 accaacaagg ccgcttccgc ggtgcgacgc tccggtcaaa cggcgatgtt tatcattctt
     6781 aacgcgatga ccagattgct tgcgccgatt ctgaccttta cgtccgagga agtctgggcg
     6841 gcgatgccgg actggaccaa caaggaagat agcgttcatc tggcgcagtt cccggaagtc
     6901 agcggcacct ttttcaatgc ggacctgggc gagcgctgga aagcgatgat cgacgcgaag
     6961 agtgaaattg ccaaggccgt tgaacaggcg cgaaaagaga aaattgtcgg ccattcgctg
     7021 gatgcgcgca tcacgattgc agcgccggaa aaactgcggg cgttgtttgc cgcgcatctc
     7081 gaggacttaa aagcgctgct gattgtgtcg cagctgcagc tggccgatga gcaggatatt
     7141 gccgcaccct tccggagtgc agaaattgaa gggctcatgg tcggtgttga gaaagcccgc
     7201 ggcgccaagt gcgaacgctg ctggatttat gacgagtccg tcggtgccga cgcgcagcat
     7261 ccgaccatct gtgcccgctg tctgccgaat ctgtaaaaaa aggagttctc tttgaaaaag
     7321 aatattatca tttttgttct gggagcggct gtcatcgtcg tgctggatca gatcacgaaa
     7381 gcggctatcg taaaaaaatt ctttctgcat gaaacccgtt cggtgattga cggttttttt
     7441 aatcttgttt acgtaatgaa tcccggcgcc gccttcggct ttctggccgg catgtccgag
     7501 atgtttcgct ctctgttttt catcggcatg accctgctgg tcatggctct gatcatttat
     7561 tacatcgcga aaagcaaatc ggaaaacatg ttcttcgtcg tttccctgac catgatcttc
     7621 gcgggcgccg tcggaaacct gatcgaccgg attcgctttg gagcggttgt ggattttctt
     7681 gatttttaca tcaaaacagc ccactggccg gcatttaatg tggctgattc agccatcacc
     7741 atcggtgctg tgttaatgat ctggggtatg gtcgcgggac gaaaacgaga ggctgtctga
     7801 tcatcacgtt ttaaacgttg agaagggtac ggccttgcac tgtggattgc aggtcacagc
     7861 attttatgtg gcggaaattt acggttgttt tgagtctttg agtttctgca attcttccct
     7921 gtagctttcc gcctgagccc ggatggtttc gatttccttt tgcgcgtgtt ccagtttttc
     7981 ttcctgcagg tgtctttcct gcctgttttt ttcctggatt tcatcgatgc ccacgtaaac
     8041 ggccaatgct cctccgagca gcaggaaaat cggaagggat cctttaaaaa tcgtcataaa
     8101 atccggccag aagaaaatta aactgaccaa tccgaagata gcggaaatga ttcccccgat
     8161 gagcaacgac atagtgttcc ctccggtcaa gatgatgatt tgaagacatt ttttttctat
     8221 cataaaaaga ccattccggc aagatgcaaa cagatgctgt gaaatgtgaa aatattgctt
     8281 gatttaaagc tgttcatcat ttaaacgtat aaaatcataa agatatgagg tgaacatgat
     8341 agagcctgat tttgccaaag gagacggtct gattcccgcg attgtccaag atgccgaaac
     8401 gaaagacgtt ctgatgatgg cttacatgaa tcgcgaatcg tgggacgcga ctttaaaaac
     8461 aggaaaggcg acctattgga gccggtcgcg gaaaaaattg tggttgaaag gcgagtcctc
     8521 gggcaatgtt cagatcgtca aagcggtttt tatcgactgc gatgaagata ccattctgtt
     8581 acatgtccaa cagataggag atgccgcctg ccataccggt caccggtcct gtttttaccg
     8641 caagttggaa ggaaatgatt ttgttgccgt cggagaaaag atttttaatc cagaggatgt
     8701 gtataaatga aaaatttaaa actgggaatt cccaagggca gtctggaagc caataccatc
     8761 gatttattta aaaaggcggg atggcgcatc accagcgacg cccgaagcta ttttcccgat
     8821 atcgatgatc cggagatttc ttgcaagctt gtccgggcgc aggaaatgtc acgttacgtt
     8881 gaagacggaa cgctggatct gggcctgacg ggtatcgact ggatcatgga aaatgattcg
     8941 gatgtcgttg tggtgcagga tcttatttat tccaaggtgt ccacgcagca ggcccgctgg
     9001 gttctggttg tccgtgacga ttctccctac aaaaagatcg aggatatgga gggaaagaga
     9061 atatccacgg aattggtcaa ctttacgaag aaatattttg cggaaagaaa catcaatatt
     9121 catgttgagt tttcctgggg agccaccgaa gccaaggttg tcgaggggct ggtggatgcc
     9181 gttgtggaag tgacggaaac gggctcgaca atcaaggcca acaaattgcg catcattcat
     9241 gaactgatga aaaccaacac tcagttaatc gccaatcgcg ctgcgtggaa aaatgcatgg
     9301 aaacgcaaaa agattgagca gatcagcacc atgctgaccg gttctttgca ggccatgggg
     9361 aaggtgggtc tgaaacttaa cgtgtccaaa gaaaatctga aaggcgttat cggcctgatc
     9421 ccctcactga aatctccgac gatttccggt ctgtcttcag acaaatggtt tgccattgaa
     9481 tcgattgttg acgcgaaagt catgcgggat ttgattccga aacttctgga tgcaggcgct
     9541 cagggtatta ttgaatatcc tttaaacaag gttatttagc ggagacaacg attatggcga
     9601 gaaaagcgaa gattgtccgt aagacaacag agacggatat tcgtcttgaa atcgatctgg
     9661 ataaaaccgc cggcagcagc attgagacca ccattccgtt ttttaatcac atgctggagc
     9721 tgtttgcgcg tcacgggttt ttgaaactga tcgtcagaag caagggggac acgcatattg
     9781 acgatcatca tctggtggaa gatctgggca tctgtctggg ccaagcggtg gctaaagcgc
     9841 tcggagacaa aaaaaacatc aatcgctacg gatcggcccg tgtgccgatg gatgaatgtc
     9901 tgtgccaggt ggatatggat atctgcggaa ggccttatct gatctatcac gtgaaaagcg
     9961 gccggagaaa aatcggcaaa tttgatccgg cgctggtcaa agaatttttc aaggcgttta
    10021 cggatcaaag cggcatcacg ctgcatatca attttctgta tggcgaaaac attcaccaca
    10081 tgattgaatc gatattcaag gctttcggcc gtgctctcag aaatgccgtg tccctaaacg
    10141 aaaacatcaa gggcgttctt tccaccaaag gaaagcttta gccggttgga ttctgctttt
    10201 ctgtggaaag cagcgtcatt catacaccga tcccttatcg gcgggacctg tcccgttgat
    10261 cgggagtcaa gacatcgagg tgagatcatg gttgccattc tccgcaaata tttttgtttt
    10321 attgtgagat ttgtttttgt cgctttgctg gcttttcctg tcatgtcgca agcgctggat
    10381 aaaaaagcac agagcgatga aagcgaaaat ctgatagcgg tcatcgcctt tcagccgtta
    10441 aggccggaag gcggcaccgg aaataccgtc ctctgtccga tttgcgggaa ggcttcggcc
    10501 ggcggcatca tacaggaggg ggcggaaaaa atcgttgaag aaatttttgt agaacaactg
    10561 aaggcaagag acgatatcgt gttggtaccc gcggaaaaat tacagaaagc gactcaaaaa
    10621 tattctaccg acagcatgaa agattttcaa gcaaccggcc tgctgaagat cggcagggaa
    10681 ttgaaggctg atttcctggt tgtcggttat gtttaccgtt ttgcggaaag ggtgggctac
    10741 cggtattcat cggaacatcc ggcgtctgtt gtttttgaaa ttcatttgat taggactgcc
    10801 gacggcaaat ccgtctggcg gggatatttt gataaaacgc aacgatctct gatggaaaat
    10861 gttctggaca tctcttcgtt tttcaagggc ggcgcaaagt ggatgacggc ccgcgaactg
    10921 acggaactgg gcatggagaa cgttttccag acgtttccgg attttcagcc ttaatccatc
    10981 atacgcggat gcggcggatc gtgaaacccc tccgtggcgg gttttagaca atgtgacgaa
    11041 aaaattaagc cttgctgttt ttgtgacatg ccgtgatttc cgactgaacc gttcagggct
    11101 cttcttattt tgaaggagtg aagcaattga ttattattcc ggcgattgat atcaaagatg
    11161 ggaaatgtgt gaggctggcg cagggcaaat ttgatcgtgt gacaacgtat gcggacaatc
    11221 ccctggatat ggcgctgctc tgggcccgaa agggtgcgga attgatccac atcgttgatc
    11281 tggacggttc ggttgccgga agaccgcgca acgcggatat cattctgaat atcgtggaga
    11341 acatcaatgt tcccgttcag atcggcggcg gcatccggga tatggagacg atccgatttt
    11401 acctggatca gggtgtctcc agtgtcattc tcggcacaac cgccctgaag aatcaacaga
    11461 ttgttcgcga agcctgtacg gtttttcccg gcagaattat tttgggcatt gacgccctca
    11521 atggaaaagt tgccgtccgg ggctggacgg aaaacacgga gaaaaatgcg gttgacctcg
    11581 cccggcagta tgaaccctat ggcgtcaagg ccatcgttta cacggatatc gagcgcgacg
    11641 gcatgggaac cggaatcaac atgaaagcga caagagaatt ggccgattcc gtcagcattc
    11701 cggtgattgc ctcaggaggc gtggcatcga ttgccgatgt ggacaacctg atggtcgtcg
    11761 aaggcggcag cattttcgga gtgattatcg gtcgcgcgct gtataccggc gccgtcgacc
    11821 tggaagaggc aataagcaaa acaaaaaaag tgacccgcac gtaacaagag gaggtggcgt
    11881 tcatgtcgga ttgtgttttc tgtaaaattg tccggggaga aatcccctgt gccagggttt
    11941 ttgaagacga gcatgtcctg gcgtttgatg atattcatcc gatggccccg gtgcatgttg
    12001 taatcattcc caaaaagcat atcgcaacgt tgatggatgt cgatgttgat aactcggatg
    12061 tgatccatcg actgacggct gccgcgcaga aagtcgcgcg aattaaaaaa atcgaccaaa
    12121 gcggtttccg cacggcgatt aactgcaatg ccgagggcgg acaggtggtt tttcatttgc
    12181 acgttcatgt gctcggcggc agaaaattgc aggacgaatt gggataacga atggaaggtg
    12241 aaaaaatgga tatcaatgcg aaattaagtg aagaaatggt tgccgctgcc aaagccaaag
    12301 acaagatcag gctttccgcg gcgcggatgc tgaaaacggc tctgcacaac aaggaaatcg
    12361 atctgatgcg tccgctccat gaaagcgaga tgctgcaggt tctctcctcg atgatcaagc
    12421 agcgaaaaga ttccatcgaa cagtttgcca aaggaggccg gactgatttg gtggaaaaag
    12481 aggaagcaga actgaagttt ttacaggaat tcatgcctgc ccagatgtcc gatgaagaag
    12541 tcgatgcgtt gatcaaaaaa acgatagagg atgccggcgc cgtttccgtg aaggatatgg
    12601 gcaaggtgat gaaaatcctg atgcccaaac tgaccggtgt ggctgacggc aaggcggttg
    12661 gtgaaaaagt gaaggctttt ttatcccaat aacatctccg ttcttcaggc tgataaaaag
    12721 cggacaaaca ggatgaatcg tgtacgttga tcaaagcaag attgaagaaa tcaaaaacag
    12781 attggatatc gtcgatcttg tttccgaata tctcaccttg aaaaaggcag gccggaattt
    12841 tatcggcctt tgcccttttc atcaggagaa gacgccctcc tttacggtga accgcgataa
    12901 gcagatcttc tactgtttcg gttgcggcga agggggcaat gtcattacgt tcctgatgaa
    12961 aattgccgac aagacttttc cggaagctat cagagacctg gcggtgaaaa cgggcgttgt
    13021 tctgcctccg gtttcctccg gaaaggaagc cagacaaaag gacttgctca aggaaaatat
    13081 cgtcagctta aattcgaaag cggcgcagca attttcgcgc aatctttttt ctgcttccgg
    13141 tactccggcc cgccaatatc ttcagcagcg gggtattgca gaggatgtcg tcaaacagtt
    13201 ccgtctcgga tacgcgccgg acacatggcg ctctctgacc gattttatgg aaaaaagcgg
    13261 cctttcgctg aaactggccg aacaggccgg cctgatcata cccggtaagg aagggggttt
    13321 ttatgaccgg tttcgggggc ggctgatctt tcccatagaa aacgttttcg gtgagatcgt
    13381 ggctttcggc gggagaattt tggggaaggg cgaacccaaa tatctgaatt caccggaatc
    13441 gccggtttac atcaagggca aaaatcttta cggattgaat aaagcgaaag acgacatacg
    13501 aaagaaggat tttgcgctga ttgtcgaggg atatttcgat ttgatttcat tgtggaatgc
    13561 cggaatccgt aatgttgtcg ccactctggg tacggcgctg accagggatc atctggatct
    13621 tctgcgacgc tattgcgtgg acgtggttgc cctgtttgat ccggatgaag cggggaaaaa
    13681 agcgcttgac cgcagtctgg aattattttt aagtacggaa atgagggccc gggctttaat
    13741 cttgcccgac ggatatgatc ctgatgatta tgtccggaaa ttcggccagg ataagatgga
    13801 agatctgatt gctcttgccc agccgctcag cgattattat attgaaaatg tcatcggggg
    13861 cggcaggacc tttgaagata aaagagatat ggtcaagacg gcgatgacgt ttctcagcaa
    13921 aatcggcgat caaaaagaaa agaacctgtt tatcaaacgt atagcggaaa aaacgggttt
    13981 gaatgaagaa ttgctgatca gagaagccta cagccgtgtt tattcgggga ggaccgaaac
    14041 aaaccggaat aacccggctg tgaatgttca tcacgatacg gtggagttgc atttgattca
    14101 actgatgatg gaatatcctc agaaaatcgt tcaggtggaa aacgagaatg tgctggatta
    14161 ttttctacag cccgatatcc gaaaactggg ggaaaaaatc atcgatgcct acaaactcct
    14221 cggcttcgtg gatgtcggtg tcattttatc gaccgaagaa aatcaggcat tgcgggaaag
    14281 aatatttcag cttatgatca aagccttgcc ggccgaggat gatgtgctgg gaaaaatatt
    14341 gaccgataac atccggcaga ttaaaaaacg gtggtttaag gagcagcatc gtcagatcaa
    14401 aataaaatta agccgggcgc aggatagcgg caacgaagag atggttcatc agctgcttta
    14461 tgaaaaagag caaatgctca taaaggaaaa ggaattgcaa tcaacaggtt gaatgaacga
    14521 cgggaggatg gctttcgcga cgcggaaggg ttcaacggat ttgttccgtc gtgtttatgg
    14581 aaccggggtt aaaaaaaaca tttgaaggag atgccagatg gcaaaagaag ttcagtccga
    14641 cgaagtcaat aagttaattt cactgggaga agaaaaaggt tttttaactt acgatgatgt
    14701 caatgacgcc ctgttgcccg atgtgacatc cgcggaacag ctggatgata ttattacact
    14761 cttcggtgaa aagaacatcg atatcattga cacggaacag ggcgaaaagc tgaccctgaa
    14821 gaagtcgatg aaggaaatta ccgacgtgaa tcttaaggaa gccctggcca ttttacccag
    14881 tgctgaaaaa acgggcgatc ccgtgaagat gtacctgcgc gaaatggggt cggtatccct
    14941 tctaagccgt gaaggggaag tggaaattgc caaaaggatc gaagagggcg cgcgcgaaac
    15001 catggcggcg atcttccgcc tgtcgatttc catcgatgag gtgctgacta tcggagagaa
    15061 actgatatcc ggcgaactca aaagcaagaa tgttgtcgac aacgtcgaag atgaagaagg
    15121 cttcattgag gaagatgagc ataggaaccg tattttgaag caaatcaata aaatccggga
    15181 acattacgat attatcaaag cgacgcgtgt taaactcaaa tcaaaaaatc ttacccaaaa
    15241 gaaaaaagat acactgcatg ccgaattgaa aagacgcata gaattcattg tcaggctctg
    15301 tcgcaagatc cgttttagca agaaacaaat ccagcgtttt attcaacgct tacggaatta
    15361 tgcagacgag attgatgctg cggagaagat tatttcaccg tacaggaaag aaacaaaaac
    15421 atccgcccgc tcgaaaaaag ataaaaaagg gacctcgcgg aaggaagcgg ttcaggcaaa
    15481 ggcacccgga aaaaatgacg cggcagtcaa ggaagcgcag aagaagataa aacaaatcga
    15541 aaaagaagtc ggcatatcgg caacgacgct taaaacaatc atcaggcaga ttgaagacgg
    15601 cgaaagaaaa gcggagagag cccagcggac gctggtggag gccaatctgc gtctggtcgt
    15661 gagcattgcc aaaaaataca ccaaccgcgg attgcagttt cttgacctga ttcaggaagg
    15721 caatatcggg ctgatgaaag cggtggataa attcgagtat cagcgcggct acaagttttc
    15781 cacttacgct acctggtgga tcaggcaggc gatcacccgt gccattgccg atcaggcccg
    15841 gacaatcaga attcccgtcc atatgattga aacgatcaat aaactgatac gcacatcccg
    15901 ttatctggtg caggaactgg gacgcgaacc cactccggag gaaattgcca ataagatgga
    15961 gtatccgctg gagaaggtga gaaaggtttt aaaaatcgcc aaggaaccca tttctctgga
    16021 aacgcccatc ggcgaggagg aagacagtca tcttggtgat tttattgagg ataaaagagt
    16081 catgtctcct tcggaagcga cgattaacat cgatctggcc gagcagacac ggaaaattct
    16141 gacaacattg acatcccgcg aggaaaaagt tttacgcatg cgcttcggaa tcagcgatcg
    16201 atcaaatatc ctcacccggg aggatatgat tgtgacagat actcttgaaa agatgccgga
    16261 acttccggaa acgcctgaga tgcccgaagt tgcagatgct ttgtcgatga gcggcacgaa
    16321 gaataaaacg cgaaaacgtt acacaaaata ataaaatgtt attgacaata tgagtatttg
    16381 tgtatatcaa ctcactcttt agaagatact cggaaaagga gcgggcctat agctcaactg
    16441 gtagagccac cggctcataa ccggtaggtc cctggttcga ctccaggtgg gcccaccaga
    16501 cccaacaaga aagatgacgg aagaaaaaaa aatatatcag gaagttcagg agatttccaa
    16561 aataagcttc ctgactgccg taatgagacg cacggagtca tcattatacg gcacagacaa
    16621 gaaacccgtg aagcagtctg ctcaaaaaat gcacccctaa aaaggtgctt tttttttaat
    16681 gacactcgct gagagcgaac gaagtgaagc tcgaagcgca agtggaatta tccccgtagt
    16741 gaggcgaatg gggattgaca ctcgctgata gtgagactcg ctgagagcaa acgaagtaaa
    16801 gctcaaagcg taaatcgaac tatccccgaa acaaagtgaa gcgggagagc aagtgaaacg
    16861 cagctcgaag cgcaagcggt agtgaggcaa gctgaacgat agtgaagcgt aaaccg
//
LOCUS       DAOOLB010000041        15693 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_3964_length_15693_cov_50.232063, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000041 DAOOLB010000000
VERSION     DAOOLB010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 15693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15693
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3964_length_15693_cov_50.232063"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="PLF77_06595"
     CDS             <1..186
                     /locus_tag="PLF77_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08556.1"
                     /translation="VANKVATDVKSAGQEVKEAAKETVVDVKSAGTEAKEAVKEKAVE
                     VKSAVNDAATDVKNAGQ"
     gene            complement(264..2006)
                     /locus_tag="PLF77_06600"
     CDS             complement(264..2006)
                     /locus_tag="PLF77_06600"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPC08557.1"
                     /translation="MDVRRTINALKDVNIAKMFTTIARLLHFRLTWTRRDLDYLPAGL
                     GPKFVSARRAAAMIPDGATLTIGGFAATGRASIFYWALRDAFDRSGHPRNLTVIGACP
                     QGGRGKTPGMIEELDAPGIITRYIVGHGETAKALLQLADDGQLELHTMSQGALAFLIE
                     AQARGLASIQTSVGLDTFLDPAVGCGSAVSPGAPNNFITAKNGKLEYRIPPIDRALFL
                     APYADKDGNIYFKDAALILDYKDAAAAARLNGGKVIVSVSKIIPRDNSQIAIPADNVN
                     AIVVNPRNEQVGGILQTQFMPHFTAGSSELDHEAVKLMRLINTFLDITPYRGKVVDIL
                     ARLCADTFVKTTGPGAIVNIGIGIGEEVARMLYESGLYRDITFTSEGGAYGGLPASGV
                     YFGAAISPQRILSTAEIFHIYEERLDTCVLGFLQVDSAGNVNVSHRGPKIIDFVGPGG
                     FTDIVEYARTIIFVGNWMDEARYGLKNGLVRVKKPGVPKFVDKVDQITFNARKALKAG
                     RKVLYVTTVGVFKLTEEGLELVQIMPGINIEKDILQNSTARFIIPGSPIPEVAPEIVS
                     GKRFELKFDKKDLA"
     gene            complement(2073..2360)
                     /locus_tag="PLF77_06605"
     CDS             complement(2073..2360)
                     /locus_tag="PLF77_06605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPC08558.1"
                     /translation="MITLIATLKVKDGNMDEAIQVLKEIVPQVKASEPGCLDYIPHTV
                     RGDDKTIIFYERYSDKDALKLHSANLMKNMEKLFPLLEPGMDVKTCYEIIR"
     gene            complement(2510..2980)
                     /locus_tag="PLF77_06610"
     CDS             complement(2510..2980)
                     /locus_tag="PLF77_06610"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPC08559.1"
                     /translation="MAEKTIIILGKSVKNFTLADQFGQDFMLSRMTGRRVLLSFHPLA
                     WTPVCTRQMQSLEKNKKVFDKLNTIAVGLSVDSVPSKAAWAKAIKVKHTKLLADFWPH
                     GGVAKSLGLFRDQNGFSQRANVILDEKGKVCFVTVYPVADLPDINEIIAFLKNA"
     gene            complement(3042..4427)
                     /locus_tag="PLF77_06615"
     CDS             complement(3042..4427)
                     /locus_tag="PLF77_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HPC08560.1"
                     /translation="MSPESISQTLTGLRAFFDAGKTQDVDARIARLKKLKEGIRRYEP
                     EIMASLYDDLRKPLFESYAGEIGILYTEIDHVIRHLPSWSRSRRAATPLIHFLSRSRI
                     DQKPYGVVLIIGPWNYPFQLVMAPLIGAIAAGNCALIKPSEFSPATSATIEKMISELF
                     DAEDVTVFQGDAQATQKLLEEKFDYIFFTGSTAVGRNIMTAAARHLTPVTLELGGKSP
                     CIVHEDAALQYGIKRIAWGKFFNAGQTCIAPDYVLIHRKIKADFTEKMIRTVFSFYGD
                     DPMRSPDYARIINERHFNRLLELLKSGRILFGGRYARKDLYISPTIIDRVSPEEPLMQ
                     EEIFGPLLPLIEYGDIDEAIAFINQRPQPLALYCFSEDKEIQEEVLQKTSSGGGCIND
                     TISHIGSQELPFGGIGASGMGAYHGKAGFDTFTHRRGILFRSNLVDIPLRYPPYRDRV
                     SILQLLFRLIR"
     gene            4683..5207
                     /locus_tag="PLF77_06620"
     CDS             4683..5207
                     /locus_tag="PLF77_06620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC08561.1"
                     /translation="MRRIFHDPVTILGSYVRRGDTAVDIGPGMGFFTIPLAKLVGPAG
                     KVIAVDIQPRMLSALAARAQKHGVAAIIRTHLAEPDSLALREKADFILAFWMAHEVPD
                     LKKFFSEIRKLMKPQGWFFLVEPLAHVSRKDFLQTIDLAKQSGLLMKECPKIRMSQSA
                     LFTPDKIERHEQPL"
     gene            complement(5449..8703)
                     /locus_tag="PLF77_06625"
     CDS             complement(5449..8703)
                     /locus_tag="PLF77_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC08562.1"
                     /translation="MIEIDMRIIFAEYGLSVLLCLIVIVSLWWQNRKRSPEIILWLID
                     YILQFAGIMLIALRGILPDFMTIVVANLFIVGGTVILYEGLCRYVEQESRQKHNYIML
                     TLFTSVHIFFTYIAPHMGLRTINFSVALFYICIQGAWLMLYRVASPLRQATKITGIVF
                     AAFCIVNIIQIIINLIKTPSDNAFVSGAAGVIAVLIYETLFFALTFSLFLLISRSLAI
                     ELENELTRRMRTAEELRQNQEKFAKAFQTSPYAISITQMKDGKFLDINDAFVNITGYS
                     REEIMKESSMGLNLWVDKEDREEMVSILRQGLPVVRKEYRFRKKNGEILTGLYSSQII
                     NITEEPCLLSSINDITEQKQAEWTLKERIKELNCLYGISAVLDEPGISLNDIMNKAVK
                     LLPAAMRFPDITGARIILEETVFQTGLFKETPWQLTEKITVNGKPEGQVNVCYPAERF
                     QTDEKPFLDSERQLLKAVAEQLGHFIEQKRAEEQIQRNATRLTGLLNIMQHRTQTTQE
                     FLDYALDEVIRLTESKVGYIYLYNENSRQFVLNSWSKDVMKECTIPNPQTRYELAKTG
                     IWGEAVRQRKPIIVNDFPADHPLKKGYPEGHAPLFKFLTVPVFDQDQIVAVVGIANKA
                     RDYDETDVLQLTLFMDAVWKSVDLKMGEAKLRKSEERFKQLAEVFPETIFEADAKGNL
                     TYANEHGLKHFGLTEQDIAQGVNIFDFVSPNDRKAAWEKIQNRIQGTQKGYLEYEALK
                     KDGSPFHALALSVPIAVDGVPVGIRGFILDITKRKQAEEALRKSEENYHFIADHTADH
                     IWTMDMFMRFTYSSPSVIKTLGYTADEFMARKIDEFFTPESLVDAQKLLAEELEREND
                     PTADPNRSRTLPSHLRHKNGHWVWLESSLTFIRDASMKPIGVLGVTRDITERKKSEEQ
                     IKHLATHDLLTDLPSLRLARDRMVSAMNMARRYKKSAAIMFLDLDGFKNVNDTLGHDA
                     GDYVLKQVAQRLLSCVRETDTVARVGGDEFLIIATEIHHPDNAAHIAEKVIQTVSQPI
                     MINGEQAVVGTSIGIALYPEHSEEMDRLIKLADEAMYKVKNAGKNNFRFVNDVN"
     gene            complement(9039..10583)
                     /locus_tag="PLF77_06630"
     CDS             complement(9039..10583)
                     /locus_tag="PLF77_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HPC08563.1"
                     /translation="MTAPLIIAMSQGKEITLLPQMANRHGLITGATGTGKTVTLQKMA
                     ESFASIGVPVFMADVKGDLSGIGAAGVASEKLLKRLEGIGVKDYQPRANTTVFWDVYG
                     KSGHPVRATISDMGPLLLARLLNLNDTQSGVLQIVFKIADDNGLLLIDLKDLRAMVQF
                     VGENAKKFKTEYGNVSAASIGAIQRGLLTIEEQGGDKFFGEPMLDISDLIQTDSEGRG
                     VMNILAADQLYNNPKLYSTFLLWMLAELFEQMPEIGDPEKPKLVFFFDEAHLLFSDAP
                     KALLEKIEQVVRLIRSKGVGVYFVTQNPLDIPDTVLAQLGNRVQHALRAFTPRDQKAV
                     QTAASTMRQNPAFDAEQAITELGVGEALISFLDEKGRPNIVERCNVIVPFSRMGAMTP
                     DERLAAIKASTIYGHYEKTVDRESAYEKLREAQQEEETPEEKPAIKVERSAAPAKSEE
                     EKDSGGMGDALSKMIFGSKGPRGGQREGMVQAAAKSVARSIGSSLGRQIIRGVLGSIL
                     GGSRGR"
     gene            10938..12161
                     /locus_tag="PLF77_06635"
     CDS             10938..12161
                     /locus_tag="PLF77_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HPC08564.1"
                     /translation="MDLQISAGDTGWILVCCSLVLLMTPALAFFYGGMVRRKNVLSTL
                     TLSYIFMSLIGVQWVLFGYSLSFGPDTGGLIGGLDYIGFQGVGAEPNAAYSRTIPHEL
                     FAAFQMMFAVITPALITGAFVERVKFKSFLMFSLLWATLVYNPLCHWVWGQGGWLKQM
                     GVLDFAGGTVVHIAAGFSALAFALVIGPRKGYGSVPMEPNNIPLTVLGTGLLWVGWFG
                     FNAGSALAANGVAVNALVTTNTSAAAAGLVWMLLSWLDGRPSTLGIATGMVVGLAAVT
                     PASGFVTPLAAMVIGAVSAPLAYYAIRFRERRKLDESLDVWACHGMGSTWGTVATGLF
                     ATVAVNADGANGMFFGNAGQLAIQITAVLVTILFSFGMTYILAKVLHVSIGLRVSPME
                     EEVGLDISSHGERSY"
     gene            12222..12599
                     /locus_tag="PLF77_06640"
     CDS             12222..12599
                     /locus_tag="PLF77_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HPC08565.1"
                     /translation="MLKKIEAIIREDKVNDVMCALKEIGIVGMSMFEVRGHGRQGGVQ
                     LVGRAGTYQVNLLPKIQLNIVLSERNLEAALETILKAAYTGETGDGIIFVTPVEDVIR
                     VRTRERGEGAMIYPGDIDERRKK"
     gene            complement(12841..13401)
                     /locus_tag="PLF77_06645"
     CDS             complement(12841..13401)
                     /locus_tag="PLF77_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08566.1"
                     /translation="MNIKGSSFVTTKAIITEAFGEERWNAFHAGLTQKDGFFNNMIMS
                     VTLIPVEKMIVFFNELCREFFNNDKTQYAMFGKSGAKFVLSPTGPYKSYMLAKDARQF
                     VELNMPKIWSTYFDGGTFTSNFDNNTAHITITGIPVKNNYFEELVMGFNQQAFKIFGE
                     KTTAVKVSSIAAGDDDFRFQLKLKDS"
     gene            13688..14107
                     /locus_tag="PLF77_06650"
     CDS             13688..14107
                     /locus_tag="PLF77_06650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPC08567.1"
                     /translation="MKISTRTRYGVRLMTALARNYGKDPVFLKNIAKEENISEKYLSL
                     IIIPLRGGGLVNSVRGAYGGYNLNKDPSEITLKEVVDVLEGDRFLVDCVNNPAACPRV
                     TTCASHDVWAVVGEKISETLSSITLDRLAKMNQKKEK"
     gene            14180..15241
                     /gene="mnmA"
                     /locus_tag="PLF77_06655"
     CDS             14180..15241
                     /gene="mnmA"
                     /locus_tag="PLF77_06655"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPC08568.1"
                     /translation="MGDKVLVAMSGGIDSSVAAWMLLNEGYNVAGVTMHFGALTEGDG
                     KRSCTSAAIDDAKKVCACLNIPHYVFDYASRLGNEVIADFISEYQKGRTPNPCVRCNR
                     HLKFGSLLDKAQELGFNYLATGHYAAIEKNEGGYCLTRPKDKKKDQTYFLYAIPYERL
                     KNILFPLAQLTKDEVRNLAMKNSLPVTAKQESQDICFVTQKNSQTFLLERVKDIAPGP
                     IVDVRGNVLGRHRGIIFHTIGQRGGLGISHPTPLYVLSIHPGNNRIVVGDKKDLMGKA
                     LIASGINRLAKSWPQQMHAKIRYRKKEALCEVREEKEKLKVIFAEEQEAITPGQSVVF
                     YDHDCVLGGGIIEKVIPFG"
     gene            15269..>15693
                     /locus_tag="PLF77_06660"
     CDS             15269..>15693
                     /locus_tag="PLF77_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HPC08569.1"
                     /translation="MKILAVNISQKKGGKKNNIGCGLFLENLGLESDAHAQADIIRQV
                     SLLAKESIEKIRAKGLDVQYGDFAENLTTEGIDLPSLPVGTRLQAGANVLLEVSQIGK
                     VCHNRCNIFYTVGDCVMPREGIFAKVLIGGEIKINDEIQ"
BASE COUNT         3958 a         3697 c         3936 g         4102 t
ORIGIN      
        1 gtggccaaca aagttgccac ggatgtcaaa agcgccggcc aagaagtgaa ggaagctgca
       61 aaagagacgg tggttgacgt caagagcgcc ggtacggaag cgaaggaagc cgtaaaagag
      121 aaagcggttg aagtcaaatc cgccgtaaac gatgcggcaa ccgatgtcaa aaatgccggc
      181 cagtaagcgc tctaagcgag atgatcaaac agacgaatcg gacattttta aagaaaatgt
      241 ccgatttttt tgcgtccgca ggttcaggcg aggtcctttt tatcgaactt aagttcaaat
      301 cttttgccgc tgacgatttc cggcgccacc tcggggatgg gggaacccgg aataataaaa
      361 cgagcggtgc tgttttgcag aatatctttt tcgatattaa ttccaggcat aatttggacg
      421 agctcaagac cttcctccgt cagtttaaag acgccaaccg ttgttacata cagtactttc
      481 cggcctgctt taagagcttt ccgggcattg aaggtaatct gatccacttt gtcgacaaat
      541 ttcggaacgc ccggtttttt aacacggaca agtccattct ttaatccgta ccgggcttca
      601 tccatccagt tccccacaaa gatgatggtc cgggcatatt cgacgatatc cgtgaaaccg
      661 cccggtccca cgaaatcaat aattttcggt ccacgatggg agacattgac attcccggct
      721 gaatccacct gtagaaaacc gagtacgcag gtatcaagtc tttcttcata gatatgaaaa
      781 atttccgcag tgctcaggat gcgctgcggg ctgatggctg cgccgaagta cacgcctgat
      841 gccggaaggc cgccataggc gccgccttcc gaggtgaagg tgatgtccct gtacagaccg
      901 gattcataaa gcattcgggc aacttcctct ccgatgccga tcccgatgtt cacgatggcc
      961 ccgggaccgg tcgtcttcac gaacgtgtcg gcgcacaggc gggcgaggat atcgacgacc
     1021 ttgccgcgat acggtgtaat atcgagaaag gtattaatta accgcatgag tttgacggct
     1081 tcgtgatcga gttcggacga gccggctgta aaatgcggca tgaactgagt ttgcagaatt
     1141 ccgccgacct gttcattgcg cgggttgaca acgattgcat tgacgttgtc cgccgggatg
     1201 gcgatctgtg aattgtcccg gggaataatt ttggatacgg agacaatcac ttttccgccg
     1261 ttgaggcgtg cggcggcggc cgcatctttg taatccagaa tcagagcagc atctttaaaa
     1321 tagatgttgc cgtccttgtc ggcgtaaggc gccagaaaaa gcgcccggtc gatgggtgga
     1381 atacgatatt ccagttttcc gttttttgcc gtgatgaaat tgtttggagc tccgggagac
     1441 accgcggaac cgcacccgac agcgggatcg agaaaggtgt ccagcccgac cgaggtctga
     1501 atggaggcaa gtccccgggc ctgcgcctcg atcaaaaacg ccagggctcc ctgtgacatg
     1561 gtgtgcagtt cgagttggcc gtcatcggcc agttgcagaa gcgccttggc ggtttctcca
     1621 tgcccgacga tatagcgggt gatgattccc ggcgcatcaa gttcctcgat cattcccggt
     1681 gttttgccgc ggccgccctg cgggcaggcg ccaatcacgg tcagattgcg gggatgtccg
     1741 ctgcgatcaa atgcgtcgcg caaagcccag taaaagatgg aggcgcgtcc tgttgcggca
     1801 aaaccgccta tggtcagcgt tgctccgtca gggatcatgg ccgccgcccg gcgggcgctg
     1861 acaaacttcg gccccagacc ggcgggtaga taatccagat cgcgtctggt ccaggtcagg
     1921 cggaaatgca gaaggcgggc gatggttgta aacattttgg caatgtttac gtccttgagt
     1981 gcgttgatcg ttcgtcttac atccattaat gctccaaaat gcctgctcta tggttacggg
     2041 gatacaaaaa ctgtcgtgac agaaaaacag gatcagcgga tgatctcata acaggttttg
     2101 acgtccatac ccggttccag cagggggaaa agtttctcca tatttttcat caaattggcc
     2161 gaatgcagtt tcaacgcatc tttatcgctg tatctttcgt aaaaaataat agtcttgtca
     2221 tcgccgcgga ccgtatgcgg aatatagtcc aggcatccgg gctcgctcgc cttcacctgc
     2281 gggacgatct ctttcaggac ttgaatagcc tcgtccatgt tgccgtcttt gactttcaac
     2341 gtcgcaatga gtgtaatcat tcattttctc ctttttttat atttcacaat ctgtggcggc
     2401 agtatatgag taaggccggt gtgtgtcaaa gaaatattta ttgaaaaata tcaaatgaat
     2461 agataacctc cccattctgt cttgccggaa aacggggagg ttgagttgat tatgcgtttt
     2521 tcagaaaagc aatgatttcg ttgatgtccg gtaagtcggc gacaggatag actgtgacaa
     2581 aacacacctt gcctttttca tcgagaatga cattggcgcg ctgcgaaaaa ccattttggt
     2641 cgcgaaacaa tcccagactt ttggccacgc cgccgtgggg ccagaaatca gccagaagtt
     2701 tggtatgttt taccttgatg gctttcgccc aggccgcttt ggacgggacg ctgtcaacgc
     2761 tcaaaccgac ggcaatggta ttgagcttat cgaatacttt tttgtttttt tcgagcgact
     2821 gcatctgccg ggtgcagacc ggcgtccacg ccagcggatg aaaagataaa agcacgcgtc
     2881 gccctgtcat tcgggaaagc ataaaatctt gtccgaattg atccgccagg gtaaaatttt
     2941 taacggactt gcccagtata atgattgttt tttcagccat gagtcacctc ccggaaaaag
     3001 ctgccccata aagcgcagcg gtttttcaga caaagaacat tctatcggat cagcctgaac
     3061 aataattgca gtattgatac tctgtcacga tacggcgggt aacgcaaggg gatatccacc
     3121 agattcgagc gaaacagaat gccgcgccgg tgcgtgaagg tatcgaaacc ggccttgccg
     3181 tgataggcgc ccataccgct ggctccgata ccgccgaacg gaagttcctg gcttccgata
     3241 tgcgaaatgg tatcgttgat gcagccgcct ccggacgatg ttttctgcag aacctcttcc
     3301 tgaatttctt tgtcctcgga aaaacaataa agcgccagcg gctgcggtct ctgattgata
     3361 aaggcaatcg cctcgtcgat atcgccatat tcaatcaagg gaagcagcgg tccgaaaatt
     3421 tcctcctgca tcagaggctc ttccggagaa acccggtcaa tgatggtggg cgatatatac
     3481 aaatctttac gggcatatct tccgccgaac agaatccgcc cgcttttcaa aagctcgagc
     3541 agacggttga agtgacgttc gttgataatc cgggcgtaat ccggactcct catcggatcg
     3601 tcgccgtaaa atgaaaaaac cgtcctgatc attttctcgg taaagtcggc tttaattttc
     3661 cgatgaatta aaacataatc cggagcgata caggtttgac cggcattgaa aaatttcccc
     3721 caggctattc gtttaattcc gtactgaaga gcggcatctt cgtgaacaat gcacgggctc
     3781 ttccctccca attccagggt taccggagtg agatggcggg cggccgcggt cataatgttc
     3841 cggccgacgg ctgtgcttcc cgtaaaaaag atataatcga atttttcctc cagaagtttt
     3901 tgcgtggcct gagcatctcc ctgaaaaacc gtgacatcct ccgcatcaaa caactcgctg
     3961 atcatttttt caatcgttgc cgatgttgcc ggtgaaaatt ccgaaggctt aatcagtgca
     4021 caattgcccg cggcaatggc accgattaac ggagccatga ccaactgaaa tggataattc
     4081 cacggaccga taatcaggac aaccccgtaa ggtttctgat caatacggct tcgcgacaag
     4141 aaatgaatga ggggtgtcgc cgcacgccgg gagcgtgacc atgacggaag gtgacggatg
     4201 acatgatcga tttccgtata aagaatgccg atttcgccgg cataggattc aaaaagaggt
     4261 ttgcgcagat cgtcgtacaa cgaggccatg atctccggtt cgtaccggcg gatgccttcc
     4321 ttgagctttt tgagccgggc gatgcgagca tcaacgtcct gtgttttccc cgcgtcaaaa
     4381 aatgcccgca gtccggtcag cgtttgcgat atggattccg gcgacatcaa catcctccat
     4441 tcaccatatg tccttcctgt gataaaagta taaggagaag agcgcagagt gtcaaaggaa
     4501 tatttccaga cgaaccattg ttttcatgga tcgcagcaat ccctgttgaa tttcactaaa
     4561 aataagcgta aagaataaaa aaatcttctc tgaaaattgt cttttgaaaa accatgactg
     4621 taaaaccgtt caacaaaaag catgtttgcc cctggtggtt gtgttttacg tttgataatc
     4681 ctatgcgaag aatttttcat gaccccgtta cgattctcgg ttcttacgtc cgccggggcg
     4741 atacggcagt ggatatcggt ccgggtatgg gcttttttac gattcccctg gccaaactcg
     4801 tcggccctgc gggtaaagtt atcgccgttg atattcaacc cagaatgctt tccgcgcttg
     4861 ccgcgcgcgc tcaaaagcac ggcgttgccg caatcatcag aacgcatctg gccgagccgg
     4921 attctcttgc ccttcgtgaa aaagccgatt ttatcctggc attctggatg gcgcacgagg
     4981 ttcccgacct gaaaaaattt ttttcagaaa tccgcaagct gatgaaaccg cagggatggt
     5041 tttttctggt cgagcctttg gcgcatgtgt caaggaaaga ttttttacag acgatcgatc
     5101 tggcaaaaca gtcgggcttg ctgatgaagg aatgtccgaa aatccgcatg agtcagagtg
     5161 ctctttttac gcctgataaa atagaaaggc acgaacagcc tctttaaaat tgatttttgg
     5221 aaatcaccgg cttcaggcac gttgccttgc ttccctgccg gcgggaattt tgtcgacaat
     5281 ccatctcggc ggcatgcatg ccggcacatt cgggagaatc aacatccggc atcgccgatg
     5341 attcaggcgg cgctgatcca tcatctctat atccgaattc actaggcagc cgtcgagctg
     5401 ccggctagga aacgccttta cgtcacggtg cggctcatgg aaggaaaact aatttacgtc
     5461 atttacaaaa cggaagttgt ttttgccggc gtttttgact ttatacatgg cttcgtcagc
     5521 cagtttaatc agccggtcca tttcttcact gtgttcggga taaagcgcaa tgccgatact
     5581 ggtgccgacc acggcttgtt caccgttgat cataatcggc tgggaaacgg tttgaatcac
     5641 tttctcagca atgtgcgctg cgttatcggg atgatgaatt tccgttgcaa tgattaaaaa
     5701 ttcatcgcct ccgacgcggg cgactgtatc ggtttcgcgc acgcaggaaa gcagacgctg
     5761 agcaacctgc ttgagcacgt aatctccggc atcatggccg agcgtgtcat tgacgttctt
     5821 aaaaccatcc aggtcgagga acatgatcgc cgctgacttt ttgtaccggc gcgccatgtt
     5881 cattgccgag accatgcgat ctctggccaa tcgtaaactg ggcagatccg tcaatagatc
     5941 gtgcgttgcc agatgtttga tctgctcttc ggattttttg cgttcggtga tatcccgggt
     6001 gacacctaaa acaccgatgg gcttcatgga cgcatcgcga ataaacgtca gagaactctc
     6061 cagccagacc caatgtccgt tcttatggcg gagatgggat ggaagtgttc gactgcggtt
     6121 cggatcggcg gtcggatcat tttctcgttc gagttcctct gcgagcaatt tctgagcgtc
     6181 aaccagcgat tcgggcgtga aaaattcgtc gatctttcgc gccatgaatt cgtcagccgt
     6241 atagcccagt gttttgatta ccgaggggct ggagtaagta aagcgcatga acatgtccat
     6301 cgtccagatg tgatcggcgg tgtgatcggc aataaaatga taattttcct cgcttttacg
     6361 cagagcctct tccgcctgct tgcgtttggt aatgtccaga ataaagccgc gaataccgac
     6421 cggtacgccg tccaccgcga tcggtaccga taacgccaga gcatgaaaag gactgccgtc
     6481 ttttttcaga gcttcatatt ccaggtatcc cttttgggtg ccctgtattc tgttctgtat
     6541 tttctcccaa gctgctttac gatcattcgg cgatacaaaa tcaaaaatgt ttacaccttg
     6601 ggcgatgtcc tgttcggtaa gaccgaaatg tttgaggccg tgttcattgg cgtacgtcag
     6661 attgcctttt gcgtccgcct cgaaaatcgt ttcgggaaac acttcggcaa gttgcttgaa
     6721 cctttcttcg cttttgcgca atttcgcctc gcccatttta aggtccaccg atttccagac
     6781 ggcatccata aacagggtga gttgcagcac atcggtttcg tcgtagtcgc gggccttgtt
     6841 tgcgatgccg accacagcga cgatttgatc ctgatcgaaa accggcacgg ttaaaaactt
     6901 gaaaaggggc gcatggcctt caggatatcc tttcttgaga ggatgatcgg ccggaaagtc
     6961 attcacaatg atcggtttgc gctggcgtac cgcttctccc cagattcccg ttttggccag
     7021 ttcataacgc gtctgcggat tcgggatggt gcattctttc atgacgtcct ttgaccagga
     7081 gttcaggaca aattgccggc tgttttcatt gtataaataa atataaccga ccttgctctc
     7141 cgtgagtctg atgacttcat cgagcgcgta atcaagaaac tcctgtgttg tctgagtacg
     7201 atgctgcatg atatttaaga ggccggtcaa gcgcgttgcg ttgcgttgga tttgttcttc
     7261 ggcgcgtttt tgctcgatga aatggccaag ctgttcggct acggctttaa gcaattgccg
     7321 ctcggaatct aaaaaaggtt tttcatccgt ctgaaaccgt tctgccggat aacagacatt
     7381 aacctgacct tccggtttgc cgtttacggt aattttttca gtgagctgcc aaggcgtttc
     7441 tttgaaaagg cctgtttgaa aaacggtttc ttccagaata atcctggccc ctgttatatc
     7501 ggggaaccgc atagccgcag gcagaagctt gaccgcctta ttcatgatgt cgtttaatga
     7561 tatgccaggc tcatccagca cagccgaaat accatagaga cagtttaatt ctttaattcg
     7621 ttctttaagc gtccactccg cctgcttttg ctcggtgata tcgttgatgc tggataaaag
     7681 acagggttcc tcggtaatgt tgatgatctg cgacgaatac aaaccggtca atatctcccc
     7741 gttcttcttg cggaacctgt actcctttcg tacgaccggc aatccttgcc ggagaatgga
     7801 aaccatttct tcacgatctt ctttatccac ccagagattc aatcccatgg atgattcctt
     7861 catgatttct tcacgcgagt aacctgtaat attaacaaac gcgtcgttta tatccaggaa
     7921 tttgccgtct ttcatttgcg taatggagat ggcataaggc gatgtctgaa acgctttggc
     7981 gaatttttcc tgattttgac gcaattcctc tgcggtccgc atccgccggg taagttcgtt
     8041 ctcaagttct atggcaagac tccggctgat cagcagaaaa agagaaaatg tcaaggcgaa
     8101 aaataatgtt tcgtaaatca ggaccgcaat gacgcccgca gcgccggata caaaggcgtt
     8161 gtcggacggt gttttaatca aatttatgat gatttggatg atattgacaa tgcaaaacgc
     8221 cgcaaaaaca attcccgtta tttttgtggc ctgacgcaac ggggaggcca ccctgtaaag
     8281 catcagccag gcgccctgga tacagatata aaaaagcgcg actgaaaaat ttatcgtgcg
     8341 caaacccata tgaggggcaa tatatgtgaa aaaaatgtga accgaggtga aaagggtaag
     8401 catgatataa ttatgtttct ggcgactttc ctgctccacg taacggcaaa gaccctcgta
     8461 aaggatcacc gttccgccga caataaagag attggcgacg acgattgtca taaagtccgg
     8521 caggatgcct cgcaatgcga tgagcataat gcccgcaaat tgaagaatat aatcgataag
     8581 ccaaagaatg atttccggag aacgtttgcg gttttgccac cagagagaaa caataacgat
     8641 caggcaaagc aatacagaca agccatattc ggcaaagatg atcctcatat caatttcaat
     8701 catggtaaca ttcctttgat ggcaacatca cgtctgttct tgaaaaatat tttatgcgtc
     8761 gtcatgtttt aaggtggtgg aaaataaggg gtcctatcct tcaattttcc cggataattc
     8821 gttggtaaag aatcaagaag tgacaaaatt acataacatg tgtgaaatct aatgattaca
     8881 gcattgcctg attgcaaatg caaaggatga aaaacaattg cttttgcggt ttttcaaaca
     8941 ttgctattga cttatattat gaattctaaa tcaatatctt gtgaaaagca attgtttctt
     9001 caccaaagcg gtgtgccgtt aaaatcggga aattttcatt agcgaccgcg ggaaccgccc
     9061 agaattgatc ccagcactcc ccggataatc tggcgtccca aagaagaacc gatgctgcgc
     9121 gccacgcttt tggccgcggc ctgcaccatg ccttctctct gtcctccgcg ggggcctttg
     9181 ctgccgaaaa tcattttgct gagagcatcc cccatgcctc cgctgtcctt ttcttcttca
     9241 ctttttgccg gagcagcgga acgctccact ttaatggcgg gtttttcttc aggcgtttcc
     9301 tcttcctgtt gtgcttcccg cagtttttca taggcggatt cgcgatcgac ggttttttca
     9361 taatgcccgt aaatcgtgga agccttgata gcggctaggc gctcatcggg cgtcatggcg
     9421 cccatgcgtg agaaaggaac aattacattg catcgttcga cgatgttcgg tcgccctttt
     9481 tcatcaagga aggaaatcag cgcttctccg acacccagtt cggtaatggc ctgttcggca
     9541 tcaaaggccg gattctggcg catcgtggaa gccgctgtct gaactgcttt ctgatcacgc
     9601 ggcgtgaagg ctctcagagc gtgctgtaca cggttgccca attgtgccag aaccgtatcc
     9661 ggaatatcca gcggattctg ggtgacaaaa taaacgccca cacccttgga acgaatcaga
     9721 cggacaacct gctcgatttt ttccagaagc gccttgggcg cgtcgctgaa caaaagatgt
     9781 gcttcatcaa aaaagaagac cagcttgggc ttttcgggat cgccgatttc cggcatctgc
     9841 tcaaacaact cagccagcat ccagagcaga aacgtcgaat aaagtttggg attattgtag
     9901 agctgatcag cggccagaat gttcatgacc ccgcgtccct cgctgtccgt ctggatcaga
     9961 tccgaaatgt ccagcatcgg ttcgccgaag aatttgtcgc cgccctgctc ttcaatggtg
    10021 agcagtccgc gttgtatcgc gccgatggag gcggcggaga catttccgta ttccgtcttg
    10081 aattttttgg cattttctcc cacaaactga accatggccc gcagatcttt caaatcgatc
    10141 agcagcaatc cgttgtcgtc ggcgattttg aagacaatct gcaaaacgcc ggattgcgtg
    10201 tcgttgagat tcagcagacg cgccagaagc aatggtccca tatcggatat ggttgcgcgc
    10261 accggatgac cggattttcc gtatacatcc cagaaaaccg tcgtgttggc ccgaggctga
    10321 taatctttca cgccgatgcc ttccagacgt ttgagcagtt tttccgaggc aacgccggcc
    10381 gcgccgatgc cggaaagatc gcccttgacg tcggccatga aaactggaac accgatggac
    10441 gcaaaggatt cagccatttt ctgcaacgtt acagtcttac cggttccggt tgctccggtt
    10501 atcaaaccgt ggcggttggc catttgcggc agtaatgtaa tttccttccc ctgcgacatg
    10561 gcaataatga gcggtgcggt catggttttc tccttggggc atatttattt aagacttcga
    10621 aagtataggg ataatgatat agcatgtcaa tgatgaaatc gtgattgtcc aggcgggaat
    10681 ttatttcaat tattctgatg gatgtttcat aaagacgtta aactgtttta cagaattgtt
    10741 acattttgta taaaaattct gatcagcgaa aggttgtcgg ctttaaactg ttacaattgt
    10801 gttctgtctt taggcgaaga gcacaaagat gtagaaattt cctcccgtgc caaattcata
    10861 accttacgta ttttataggg aatgtgtgat ggcaggatta ttgctaatca ttaattattt
    10921 tcatcgggag gaacattatg gatctacaga taagcgcggg cgatacagga tggatactgg
    10981 tttgctgttc tcttgtgctc ttgatgaccc ccgccctggc gtttttttac ggcggcatgg
    11041 tcagaagaaa aaatgttcta tccaccttaa cgttaagtta tatctttatg tcgctgatcg
    11101 gcgtgcagtg ggtgcttttc ggatattctc tttctttcgg cccggatacc ggcggactga
    11161 tcggcggatt ggattacatc ggctttcagg gcgtcggcgc cgaaccgaac gccgcttatt
    11221 ctagaaccat tcctcatgag cttttcgccg ctttccagat gatgtttgcc gtcattacgc
    11281 cggcgctgat tacaggggct tttgtggagc gagtaaaatt caagagcttc ctgatgttca
    11341 gcctgctctg ggcaacattg gtttacaatc ctctttgtca ctgggtctgg gggcagggcg
    11401 gatggctgaa acagatgggc gttctggatt ttgccggagg aacggtcgtt catattgccg
    11461 ccggtttttc cgctctggcg tttgcactgg ttatcggtcc gcgcaagggc tacggaagcg
    11521 ttccgatgga gccgaataat attccgctta cggttttagg aaccggtttg ctttgggtcg
    11581 gctggttcgg gtttaatgcg ggcagcgctc tcgcggccaa cggcgtggcg gtgaacgctc
    11641 tggtgacgac caatacgtcg gcggcggccg cggggctggt ctggatgctg ctttcctggc
    11701 tggacggaag accgagcacg ctgggcatcg ccaccggcat ggttgtagga ctcgcagccg
    11761 tgacgccggc cagcggtttt gtaacgcctc tggccgcgat ggtgatcgga gccgtgtctg
    11821 ctcctcttgc ttattatgcc atccgtttcc gggaacggcg caaactggac gagtctctgg
    11881 atgtctgggc ctgtcacggc atgggcagca cctggggcac cgtcgctaca ggattatttg
    11941 ctaccgtggc cgttaatgcc gacggcgcca acggcatgtt tttcgggaat gccggccaac
    12001 tcgccattca gattaccgcc gttttggtca ccatactctt ttcgttcggc atgacctata
    12061 ttctggccaa ggttctgcac gtgagcatcg gattgcgcgt cagtccgatg gaagaagaag
    12121 tgggactgga tatcagttca cacggagaac ggtcctacta acagaacatg cttgaatgga
    12181 aacattgatc agttttttat ttttaaaccg gagaggttac catgctgaaa aaaatcgaag
    12241 ccattattcg cgaagataaa gtgaacgatg tcatgtgcgc cctgaaagag attggcattg
    12301 tgggtatgag tatgtttgaa gtgaggggac acggccgcca gggcggcgtc cagctggtcg
    12361 gacgcgccgg tacctatcag gtcaatctgc ttcccaagat acaactcaat atcgttttaa
    12421 gcgaacgcaa cctggaagcg gctctggaga cgattcttaa agcggcctac accggcgaga
    12481 cgggcgacgg aatcattttt gtcacaccgg tagaggatgt cattcgcgtc cgcacgcgtg
    12541 agcgcggaga gggtgcgatg atatatccgg gtgatattga cgaaagaaga aaaaaataaa
    12601 cagagagatt tgattttctg ctgattagga aacctgagaa aaaaataaag tgccgattca
    12661 ttgaagtatc ggtgcagata aaacatcctt attttttcat tatccttttc atcgataaca
    12721 aaaaaatccg gaccacccga tcccggaaga ctaatgaatg gtttagccac aaattcatgc
    12781 ctggtttaat atagaaagct tttctcgcaa tcccgcgaaa gcaaccattt atgtttgtgg
    12841 tcatgaatcc ttcagtttta gctgaaaccg aaaatcatca tcacctgctg cgatgctact
    12901 gactttgacc gcggttgttt tttcaccgaa tattttgaac gcctgctggt tgaatcccat
    12961 aacgagctcc tcgaaatagt tattcttcac tggaattccg gtgatagtga tatgagcggt
    13021 gttgttatcg aaatttgacg taaacgtacc gccatcaaaa taagttgacc agatttttgg
    13081 catgttcagt tcgacaaact gcctggcatc cttggcaagc atataagatt tgtagggacc
    13141 tgtcggagag agaacaaatt tcgcgcccga ttttccaaac atggcgtatt gagttttatc
    13201 attgttgaaa aattccctgc atagttcatt aaaaaaaaca atcatcttct ctaccggaat
    13261 aagggtaacg gacataatca tattgttgaa aaaaccatct ttttgggtaa gaccggcgtg
    13321 aaatgcattc catcgctctt cgccgaacgc ttctgttatg atagccttgg tcgtgacaaa
    13381 ggaacttccc ttaatattca tattaagcgc cctccataaa tttttttgta aatttaatac
    13441 tcaaggtatg aggttgcaag taaaaatatt aattcgttga tcaggtacga aatgtttttg
    13501 cgtatattga atgcatggga ggtgcgggta taatcttcat gaattaagtt tttagaaaga
    13561 taaatcgaag aggccgacga tattgccgtt tcataaaaca atcattttct cttgacacga
    13621 catatctgtt actttaatta ctagtataat gatagtatca gtaagtatga tgtaaaagga
    13681 cagcgtaatg aaaatttcaa cacgaacgcg ctacggcgtc cgactgatga cggccctggc
    13741 ccgcaattac ggcaaagacc ccgttttcct caaaaatatt gccaaagaag aaaacatatc
    13801 cgaaaaatac ctgagtctga ttattatccc tttgcgaggg ggtggtctgg tcaattcggt
    13861 ccgaggagct tacggcggat ataacctgaa caaagatccc tcggagataa ctttaaagga
    13921 ggttgtcgat gttttggaag gagaccgctt tctggttgat tgcgttaata atccggccgc
    13981 ctgtccccgc gtgacgacat gtgccagcca tgatgtctgg gcggttgttg gagaaaaaat
    14041 ttcagaaacc ttgagctcca taacccttga tcggcttgcg aagatgaatc agaaaaaaga
    14101 aaaataatta tgcagaatat ttgaaagaaa agataactgc tgcaaaacat gaatttgata
    14161 tttgatcctg aggataagaa tgggtgataa agttttggtg gccatgagcg gcgggattga
    14221 ttcatcggtg gccgcatgga tgctgcttaa tgaaggttac aatgttgccg gcgtgaccat
    14281 gcattttggc gccttgaccg agggagacgg gaaaagatcc tgcacgtccg ccgcgattga
    14341 cgacgcaaaa aaagtttgcg cctgtcttaa cattccccat tatgtttttg attacgcctc
    14401 ccggctggga aatgaggtca ttgccgattt tatcagcgaa tatcagaaag ggagaacgcc
    14461 taatccctgt gttcgctgca accgccatct gaaattcggt tcactgctgg acaaggcgca
    14521 ggagctgggt ttcaattatc tggcgaccgg ccattacgcg gccattgaaa aaaacgaagg
    14581 cggttattgt cttacaaggc cgaaagataa aaaaaaagat cagacgtatt ttctctatgc
    14641 cattccctat gagagattaa aaaatatatt attccccctt gctcaactca cgaaggatga
    14701 agtgagaaat ttggcgatga aaaattctct gcccgtgacg gcaaagcaag agagtcagga
    14761 tatttgtttt gtgactcaga aaaattctca gacatttctt ttggaaagag taaaagacat
    14821 cgcgccgggt ccgattgttg atgtgcgggg caatgtgctg ggcaggcatc gcgggattat
    14881 ctttcatact atcggccagc ggggcggtct gggtatcagc catcccacgc cgttgtatgt
    14941 attgtccatt catcccggga acaaccgcat cgttgtcggt gacaagaaag acttgatggg
    15001 caaagcgctt atcgcgtccg gcatcaatag gctggcgaaa tcctggccgc agcagatgca
    15061 tgcgaagatc agataccgga aaaaagaagc gctttgcgaa gtccgggaag aaaaagaaaa
    15121 attgaaagtt atctttgccg aagaacagga ggcaattaca ccgggtcaat cggtagtctt
    15181 ttacgatcac gattgcgttc tgggcggcgg gatcattgag aaagtcatcc ctttcggata
    15241 aggcaaaata caggtaaaag aaacaaaaat gaaaattctg gcggtgaaca tctctcagaa
    15301 aaaaggggga aaaaagaata atatcggctg cggtcttttt ttagaaaatc tggggctgga
    15361 aagcgatgcc catgcccagg ccgatatcat ccggcaggtc agtcttctgg ccaaagaaag
    15421 cattgaaaaa atcagagcca aaggccttga cgttcaatac ggcgattttg ccgaaaatct
    15481 gacgaccgaa ggaattgatc tgccgtcgct tcctgtcggt acaaggttgc aagccggcgc
    15541 caatgttctg ctggaggttt cgcaaatcgg caaggtctgc cataatcgat gcaatatttt
    15601 ttatactgtg ggcgattgcg tcatgccccg ggaaggtata ttcgctaaag tgctcatcgg
    15661 cggagaaatt aaaatcaatg atgaaatcca aat
//
LOCUS       DAOOLB010000042        15635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_3987_length_15635_cov_40.262773, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000042 DAOOLB010000000
VERSION     DAOOLB010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 15635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15635
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3987_length_15635_cov_40.262773"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="PLF77_06665"
     CDS             complement(<1..1663)
                     /locus_tag="PLF77_06665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HPC08570.1"
                     /translation="MEQQNRKSSENEEQYGSLLEAIEEGYYEVDLAGNFTFFNKAMCR
                     ILGYAPDEMLGMNNRQFTDKENAKKLFETFNRVYKTGESTKAFDWQLIRKDGNKKYIE
                     TSVSLIKDASGKPTGFKGIVHDITERKQAEEALRESEALYRLLADNISEHVWVMDLNL
                     NLIYISPSVEKIYGYALDEIRQIKMRQLFTADSYRKVVETFMKELEKAMNSKPPPTGT
                     NYIMELEAYHKDGHKIWIENRLHFIRDKNGKPISLLGETRDITDRKLAQEALKASEEK
                     YRLLADHMKDQVWMMDMNMQITYVSPSVERITGYSFEEIKILPLDQLLTPDSVRKATE
                     FITNRMPKAFNLVSLDSQYRTLELEFRTKEGKTIWGECSFNFIRNENNKPISILGEAR
                     NITDRKIAEDKLRQSEEKYRTILEDIKEGYFETDLFGNFTFFNDTVCRVLGYSRNELT
                     GMSNKIYTDAEERKKVYRAFHQVYMTGEPYRDLEWQIRGKDGTSKYIEGFISLLRDSS
                     GNPKGFRGIAHDITERKIADEKLQKTLESLKKAVGTTIQVLISALE"
     gene            complement(1950..2150)
                     /locus_tag="PLF77_06670"
     CDS             complement(1950..2150)
                     /locus_tag="PLF77_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08571.1"
                     /translation="MTKNEISREEIIKKLVDSDLDGLSQEKSIKFLVRILHNGWKGYN
                     EMSDAELLEAYNHREFRKTVRT"
     gene            complement(2315..3547)
                     /locus_tag="PLF77_06675"
     CDS             complement(2315..3547)
                     /locus_tag="PLF77_06675"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPC08572.1"
                     /translation="MEDIKITPADVQETLCFTGNIIEESGARLAGSESCKKAAVLIMN
                     EMNKHCDSVSMEEFDIHPKAFLGFFKVVVVIYILSSFLLYFDYVVAGAAGYLLGAFIM
                     LGESIFYWEMLDPFYRKMKGYNVIGTIEPEGEVKQQIILSGHHDSAHEFRFLAHHQKL
                     YAVRIMMAVITIFAALVLSWIWVYYRSYSGVDPAFVPYLRHGVFAGLIFVGPMYFFIS
                     EKGTPGAGDNMIAVAAIIKLAEIFNRAKQNGAGLLKNTRLMFLSADAEEAGLRGARAF
                     VKRHRGALLKMPTYHFNIDSIYNLGELQFLTRDINSVVRLSKNMARECKAVADQLGYR
                     TRLFAITPGGGATDAAEFAKAGVEATTLIGMPTNLIRDGMVYHTLQDNVEHIEPEAVE
                     ATLKIAFVYILNKERDNI"
     gene            complement(3773..4354)
                     /locus_tag="PLF77_06680"
     CDS             complement(3773..4354)
                     /locus_tag="PLF77_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08573.1"
                     /translation="MKNKVYKALLLTLIVLMPVSAMADVRVHVNIPLPLPIVFPSPPH
                     LVVIPDTDVYAVADIGEDIFFHAGWWWRPWNNRWYRSRYYDRDWAYHPYQPSFQRYVP
                     ADWRRNYRHKTWNGYRWEPQRRSYQDIQRNWYVWEKNRHWDKQSYGVKKITKRAPAPQ
                     YRSNTRPAMTKPAMQHNVKSRGDFRTSERRDRR"
     gene            complement(4433..4918)
                     /locus_tag="PLF77_06685"
     CDS             complement(4433..4918)
                     /locus_tag="PLF77_06685"
                     /inference="COORDINATES: protein motif:HMM:NF025173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HPC08574.1"
                     /translation="MKKITFTMIAVFLGLLLTSQAFAWGPGPGKKSGYCRGVVLEELN
                     VTEDQKTKLDALQSQHFKAVHPLREKIRDKSAELRKLWLQVDPDKNKIMAKQKEVRVL
                     RDQLEDNKTAYRFEVNKILTPKQKEKLAKCGWNKKSGFGPRGGKRGEQWRGHGNCLYL
                     P"
     assembly_gap    4971..5070
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5298..5837)
                     /locus_tag="PLF77_06690"
     CDS             complement(5298..5837)
                     /locus_tag="PLF77_06690"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC08575.1"
                     /translation="MFRNIVYVLLMIIFSGSLAMGETSTAADWFNRADGLWDGKKYVE
                     VKEAMAYLDKAIEQQPNYANAYISRGMAYNQTGDYQRAIQDYNLALLFTNDENSLTKI
                     FDKMGVAYANLGQYQRAIDNFNHAISLKPDYADAYNNRGIVYFLQGDKKQCCNDAQKA
                     CELKNCELLSWARERGACP"
     gene            complement(6078..6971)
                     /locus_tag="PLF77_06695"
     CDS             complement(6078..6971)
                     /locus_tag="PLF77_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08576.1"
                     /translation="MAMKLLNNRNFIFILAMILGLFLPQPAEWTKVLMIPALALVMTL
                     ATVNVPNNYFLNPRAILKPSLAGMFMSYIILAGVLLCLSALFIEDKNLWIGFVLIASV
                     PPAVAVIPFTAILEGNVDYTLSGTVASYIAALLIMPVVFWIFIGTVFEDPYKLLQIML
                     LLIVLPLILSRLILYFNWQEKLSSVRGFMTDWGFFIVLYSMIGVNRDLILSNPFTVFS
                     VAVISFCTTFVLGSVIQRICAFCKIDRKNMVSLVLLGTLKNQGIAGGLAISLFEKEAA
                     IPSAIYSIFMIIYIMWLDWRK"
     gene            complement(7357..7572)
                     /locus_tag="PLF77_06700"
     CDS             complement(7357..7572)
                     /locus_tag="PLF77_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08577.1"
                     /translation="MNEEIDDDLIDIELEGDALEKGMRKLESEAERLLHYTNDLLQEL
                     NKERAKREAMKQEDEKTQSTEEGSAST"
     gene            complement(7754..8188)
                     /locus_tag="PLF77_06705"
     CDS             complement(7754..8188)
                     /locus_tag="PLF77_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08578.1"
                     /translation="MQKRNDRQPYSLESMVELYPRLYSQKRLDEWMDLFDKRAIMVRV
                     QKGKPVSFVSITEGITEQREYAAENALFDEKWHHVKINRHGNIAVIKANYILTAGREI
                     RKGIDVLTLCCRDGGWRIVCLAYEQKGMVRRSIPRKDSVKTS"
     gene            complement(8254..8754)
                     /locus_tag="PLF77_06710"
     CDS             complement(8254..8754)
                     /locus_tag="PLF77_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08579.1"
                     /translation="MVKRKYLAMGLILVAAFAVYLLFFVDWEARAVKKHLQALAKELE
                     WSTGENELAVAHRIRRIQEGMAENCQVDIPSYGISRAVVRHDVPVYLMTARGYYKNLS
                     VELEDLEIETLALPAAQAVATAYVKAIQSDGVRNDEVLVIRFRLQKVNKKWQVNAAGE
                     VHVLEK"
     gene            complement(8748..9245)
                     /gene="lepB"
                     /locus_tag="PLF77_06715"
     CDS             complement(8748..9245)
                     /gene="lepB"
                     /locus_tag="PLF77_06715"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPC08580.1"
                     /translation="MNKTAIRRFFFPDITARYVLRLAVVAAVAFILFKYVFIPFRIQG
                     ESMAPTYVTGSFNFCFALRYMFASPAPPDVVTVRMAGQRIMLLKRVVAVSGQRVEFRK
                     GILFVDDKKINEPYVKKKSNWNLEPIIVKPGHVYVVGDNRAMPAETHTFGQAPVSRIV
                     GVPLW"
     gene            complement(9283..9453)
                     /locus_tag="PLF77_06720"
     CDS             complement(9283..9453)
                     /locus_tag="PLF77_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08581.1"
                     /translation="MDKVANEIGHYIIRAFDAMGAEIIGDVFRWSGVTETGYGAIFIV
                     LVILVVIYIVKK"
     gene            complement(9547..10875)
                     /locus_tag="PLF77_06725"
     CDS             complement(9547..10875)
                     /locus_tag="PLF77_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPC08582.1"
                     /translation="MQIKNYKILIIGSGPAGLFAALRLEKLGMDDIAVIDRNPYPAGG
                     LLNDGKLNFDYRVGLDIDELQIDRVTAEGMMLEIKRIFTGFPRCRQVTFTDNRNNLRA
                     LADMAAEHGAQFIAPEQWHWGTDNGRALVDYLRGQLKKTDFLLSTEVESVEKKREGFF
                     RLFCRINKKRISCHAPVILAAPGRSGAYWFRDIARALEIRNNFGPIDVGIRLELNRKF
                     YDFVTGIVYDPKFIFTTGRHGDKVRTFCTNPGGRVRVENYGDFKLVNGDALSGKKTKN
                     TNFALINTVALTKPYSDTTEFGLSIAKQFFLLGGGKPIVQRIGDFREGRRSSLSTFNS
                     TARHFEVCRATCSATPGDITLAMPARIMDNLWESLKSLDKIIPGILHPSTLMYAPEIK
                     FFDTHFPTDRYLETNVKGIFVAGDGTGKSRGIVGAAMSGMIAAEGIMNGR"
     gene            11229..11675
                     /locus_tag="PLF77_06730"
     CDS             11229..11675
                     /locus_tag="PLF77_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HPC08583.1"
                     /translation="MFAPKRILVPTDFSDYSDEALKQALELAKQYHSKVYLLHVANPV
                     TQCTADYCLDVDTFKKAEESTMIKARDMMQQELQKFPESREIEIISDIREGEPVAEIL
                     KEQEEKGVDLIVMPSHGRSGLLKRLMGQIAEKVMEATKTPVLLVHH"
     gene            complement(11741..13438)
                     /locus_tag="PLF77_06735"
     CDS             complement(11741..13438)
                     /locus_tag="PLF77_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HPC08584.1"
                     /translation="MLSIKKIGVLGRTYRNFSRYRQILSILFRYGFDDLVERLKIDQY
                     IEIGLQMISRHQSENVERLSRPERVRLLFEELGPTFIKFAQILSTRPDLVPADVIREF
                     EKLQDNVPPFPFSEAKAIIESELGGDLDNFFSSFDEKPLASASIAQVHKAVLNDGEVV
                     AVKIQRPGLKKIIEVDLEIMLHLASLMEKNIEEISFQKPTRVVEEFARTLERELDFTT
                     EASSMERVAGQFLSDRTIYIPKVFSELSGRRVLTMEYVDGIKVSDMEKLENAGMDLKK
                     ITSRGAHFVIKQVFEFGFFHADPHPGNVFVLPNNVICVLDFGMTGSVDKRQRALFVDI
                     METLGRKDAEKCARLMLELGEYDDEPDIRLLEREIDDFMGRHLFKSLKDIDLARLIQD
                     LLDIATSNKIRIPPVIFLMMKAFAAMEGIARLLDPEFDMIAYAEPFIKRAKLARYAPE
                     KIAADLLTVVSESMHMIQTLPREVLQVTRLIRQNKMTINMEMRWIDTFLRDLDQASNR
                     LSFSIIIAALIIGSAYLLAYSTPPLVYGISLIGLIGFSAAAVLGGWLLIAILKKGML"
     gene            complement(13506..13805)
                     /locus_tag="PLF77_06740"
     CDS             complement(13506..13805)
                     /locus_tag="PLF77_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08585.1"
                     /translation="MPFEMMKKVMLTGIGLALKTRSEMEAVAKDIIKKSKMSEAEGRK
                     FVADLTKKYEQSRRDMEKTIQKGIADYMAGADIASRKELNALKKEIGNLKKARKK"
     gene            complement(14016..15035)
                     /locus_tag="PLF77_06745"
     CDS             complement(14016..15035)
                     /locus_tag="PLF77_06745"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HPC08586.1"
                     /translation="MGCFERRETHTIMLDDVYHGYLDWNMMFAAERLGRLDAQKTGIA
                     AYLARDSYYNDPRYAETLLTLGFFDVQLFYHNHLQALIGCRDIQTPEGAKALVVIVFR
                     GSDQVQDWLQNLDIRMTRFLNKEKFPHLSRLKVHNGIFERVHDFEDWSRRVIFKDGPL
                     KGNLLQMLGDELKRKKCFFWIIGHSLGGAMATLYAARLCDCYNVSRNRILSHTFGAPP
                     IGNRYFAARYGHESCLRTKKSKRKSDSLALFRFVNTEDPIPAPRFADVRVEKAQGGPI
                     AQPPYRLLGFKHVGKEVRFSPRHLPGFYEKYQQFTGRRYNSSHFMKVHFMEAYITGID
                     VLNTH"
     gene            complement(15099..15335)
                     /locus_tag="PLF77_06750"
     CDS             complement(15099..15335)
                     /locus_tag="PLF77_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amphi-Trp domain-containing protein"
                     /protein_id="HPC08587.1"
                     /translation="MPQKQKKKKRDIEKDYTAGQFVMKLRRLADCIETGRPFRIQIAG
                     EKITIPTDAVINIEHERGTSEEEVEFQIKWKLNR"
     gene            complement(15414..>15635)
                     /locus_tag="PLF77_06755"
     CDS             complement(15414..>15635)
                     /locus_tag="PLF77_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08588.1"
                     /translation="GNPQHVKTNEFNASQPVVGRKIKVVFKDGEVLIGTTTGYQPGRP
                     GFFLVPADAGSNNERCYVVTASTKEISFL"
BASE COUNT         3990 a         3888 c         3276 g         4381 t
ORIGIN      
        1 cttccagggc ggaaatgagc acctgaatgg tggtgccgac ggcctttttc aggctctcca
       61 gtgttttttg cagcttttca tcggctattt ttctctccgt tatatcgtgg gcaatgcctc
      121 tgaatccttt cgggttaccc gaagaatctc tcaaaaggga tataaatcct tcaatatact
      181 tgctcgtgcc gtcttttcca cggatttgcc actccagatc cctgtaaggt tctccggtca
      241 tataaacctg atggaaggcg cgatatactt ttttccgttc ttccgcgtcc gtgtagattt
      301 tattactcat acccgtcaat tcgtttctgg agtacccaag gacccggcac acagtatcat
      361 taaagaacgt aaagtttccg aagaggtcgg tttcaaaata gccttcctta atgtcctcca
      421 gaatcgtcct gtacttctct tcactctgac gcagtttatc ttcggctatt ttgcgatccg
      481 tgatatttct cgcttcaccc aaaatggaaa tcggtttatt attttcattg cgtataaaat
      541 taaatgaaca ttccccccat atcgttttac cttcttttgt ccggaactcc agttccagtg
      601 ttctatattg agaatccaga gaaaccagat taaaggcttt cggcatcctg ttggtaataa
      661 actccgtggc ctttctgacc gaatcgggag taagcagttg atccaaaggc aatattttta
      721 tttcttcaaa cgaatatccc gtgattcttt ccacggacgg gctgacatag gtaatctgca
      781 tgttcatatc catcatccag acctgatctt tcatgtgatc ggccagaaga cgatattttt
      841 cctcactcgc tttcagagct tcctgagcaa gtttgcggtc ggtgatatcc cgggtttctc
      901 ccaaaagaga tatcggcttt ccatttttat cgcggataaa atgcaggcgg ttctcgatcc
      961 atattttatg gccgtccttg tgataggctt ccagttccat gatatagttc gtaccggtag
     1021 gaggaggctt gctgttcatc gctttctcca attctttcat gaatgtttcc acgactttcc
     1081 tgtaggagtc tgccgtaaaa agttgcctca ttttgatctg tctgatttca tccagggcat
     1141 atccgtatat tttctccacg gaaggactga tgtagataag gttgaggttc aaatccataa
     1201 cccagacatg ttccgagatg ttgtccgcca acagccggta aagcgcttca ctttctctca
     1261 gtgcctcttc cgcctgctta cgttcggtga tatcatgaac aatgcctttg aaacctgttg
     1321 gtttccccga agcatccttg atcaaggata cggaggtttc aatatatttt ttatttccgt
     1381 ctttcctgat gagctgccaa tcgaacgctt tggtcgattc tcccgtttta tagaccctgt
     1441 tgaaggtctc aaaaagtttt tttgcatttt ctttgtcggt aaattgacgg ttattcattc
     1501 ccagcatttc atccggagca tatcccagaa tccggcacat cgccttgttg aagaatgtaa
     1561 agttgccggc aagatccact tcatagtatc cctcttcaat ggcttccaga agggatccgt
     1621 attgttcttc gttttcactt gactttctgt tttgctgttc cattttttcc tgtcttcctt
     1681 acatgtggaa atggcgccga tcaatgtcgg gttcagacct ttattcattc atcatgaaaa
     1741 cggtatataa ataacggttt tgtgtcaaag aataaaattc cggatacttt cagttttgga
     1801 tattgaaacg tatcatccga ttgttattca gaaagacccc gatgacgcta tttccccaat
     1861 accgtcagaa cggtgaaaca atataccttg catcaacatc ggcctgttca cacaaatgat
     1921 caaatttata tatactgttt tgatatccat cacgttctga ctgttttcct gaattctctg
     1981 tgattatacg cctccagcag ttccgcatca gacatttcat tatacccttt ccagccgtta
     2041 tgaagaatac gcaccagaaa tttgatgctc ttttcctgcg acaagccatc caaatcagaa
     2101 tctaccagtt ttttaatgat ctcctccctg ctgatttcat tttttgtcat aaacctgttc
     2161 ctccctgata aaaaaaataa accggacatc acaacaggat ttattaatca atgcgcgaca
     2221 acttcacgga aggttgaatg gttaaataat ttgcatctat gatactaatt gtattaagtt
     2281 tagcacggct tttctgaaaa atccagcatc tgatctaaat attatcccgc tccttgttca
     2341 gaatatagac gaacgcgatt ttaagtgtgg cttcaacagc ttccggctcg atatgctcaa
     2401 cgttgtcctg aagagtgtga tagaccattc cgtcccggat taaattcgtc ggcataccga
     2461 tgagcgttgt cgcttctacg cctgctttgg caaattccgc cgcatccgtg gcgccgcctc
     2521 cgggcgttat tgcaaagagc cgcgtccggt atccgagttg atcagccact gccttgcact
     2581 cccgggccat gtttttagac aaacgaacga cgctgttgat atctctggtg agaaactgga
     2641 gttcaccgag gttgtagatg ctgtcgatat tgaaatgata cgtcggcatc ttcagcaagg
     2701 cgccccgatg cctttttaca aaagcccgcg cgcctcgaag accggcctct tccgcatcgg
     2761 cgcttagaaa catcagacgg gtatttttca gaagccccgc accgttttgc tttgccctgt
     2821 tgaaaatttc cgcaagttta ataatcgccg cgactgcaat catgttatca ccggctcccg
     2881 gagtgccctt ctcacttata aagaaataca tcgggccaac aaaaatcaga ccggcaaaaa
     2941 caccgtggcg taaataggga acaaaagcgg gatcgacgcc gctataggac cggtaataca
     3001 cccaaatcca ggagagaacc agtgcggcaa aaatggttat cacggccatc atgatccgga
     3061 cggcatataa tttctgatga tgtgcgagaa acctgaattc atgcgcgctg tcgtgatgcc
     3121 cgctcaggat gatctgttgt ttgacctcgc cttccggttc tatggttccg ataacgttat
     3181 accccttcat tttcctgtaa aaaggatcca gcatttccca gtaaaatatt gattcgccca
     3241 acatgatgaa agcacccaaa aggtagccgg ccgcaccggc aacgacataa tcaaaataca
     3301 gcaaaaacga ggacaggatg tagatcacga cgacaacctt gaaaaacccc agaaacgctt
     3361 tggggtgaat atcaaactcc tccatactta cggaatcgca gtgtttattc atttcattca
     3421 taatgagaac agccgctttt ttacaggatt cactccccgc gagacgcgca ccggattcct
     3481 ctataatgtt cccggtaaaa caaagcgttt cctgaacatc agccggtgta atttttatgt
     3541 cttccacagc atgcctcctg gttcccgaaa attaaaaccg atctgcccat aaatgctgaa
     3601 gctttcccga actattttct attatgatgc tttacgtaac ataaaagacg taaagaaaga
     3661 agtgatgagt tcggttggcg acaatccggg aagtgcttat tgaaaaaacg accagccttg
     3721 atcttttaaa atcaaggctg gtcgtatatc ccgcgtttta gcggttgact cgttaacgtc
     3781 tgtctcttct ttcggacgtc ctgaaatcac cccggctttt gacgttatgc tgcatagcgg
     3841 gcttcgtcat ggccggtcgc gtgttgcttc tgtattgcgg cgctggggct ctctttgtta
     3901 tttttttaac gccatagctt tgtttatccc agtgccggtt tttttcccag acataccaat
     3961 tgcgctggat atcctgatat gacctccgct ggggttccca gcgatatcca ttccacgttt
     4021 tatgcctgta attccgtcgc caatcggcag gaacatatct ctggaaagac ggctgatagg
     4081 gatgataagc ccaatctctg tcgtagtatc ttgatcggta ccaacggtta ttccaaggac
     4141 gccaccacca gcccgcgtga aaaaatatgt cttctccaat gtcggctacg gcataaacat
     4201 cagtgtccgg tatgaccacc agatgcggcg gcgacggaaa gacaatcggc agaggcaaag
     4261 gaatattcac atggaccctt acatcggcca ttgccgaaac cggcatcaat acaatcagcg
     4321 tcagcaacag agctttgtaa accttatttt tcattttatg ttctcctttt tgtttatcct
     4381 aataacatgt tgcatcatcc atatttccgg aatgaaaaaa acatccggtt cattacggta
     4441 agtagaggca attcccatga cccctccatt gctcacccct tttcccgccg cgcggaccga
     4501 atccgctttt tttgttccat ccgcatttgg ccagtttttc cttctgtttg ggtgtcaaaa
     4561 ttttgttcac ctcaaagcgg taagcggttt tattatcctc cagttgatcc cgcagtaccc
     4621 ttacttcttt ttgtttggcc ataatcttgt ttttatccgg atcgacctgc agccataact
     4681 tacgcaattc agcagatttg tcgcgtatct tttcccgcag ggggtgcacc gccttaaaat
     4741 gctgcgactg caaggcatca agttttgttt tctgatcttc agtcacattc aattcttcca
     4801 aaacgactcc ccgacaatat ccggactttt tccccggacc cggcccccac gcaaatgcct
     4861 gagacgtcaa aagcaacccc agaaatacgg cgatcattgt aaatgttatt ttcttcatca
     4921 cttgtctcct ttttttaaag ttaccctctt gaacagcaaa gcccgtgcca nnnnnnnnnn
     4981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaaaaaggt atcctcgcca ctatgcatac
     5101 aggatgggat gcatagctta aacaccttgg ggggattcgg cgaggatgat ttcttataat
     5161 acaaatataa taatttatca acctatataa aataaaattt tccgctaccc tgttattatc
     5221 acataacatc acagaaaccg gagtgaaaaa aaagaccctg tgcataagaa ggcgattccg
     5281 gatatcggcc attttcatca gggacaggcg ccccgctccc tggcccatga caataattcg
     5341 cagtttttca gttcgcatgc tttttgcgca tcattacagc actgcttttt gtcgccctga
     5401 agaaaataaa caatgccccg gttattgtac gcatcggcat aatcaggctt cagactgatg
     5461 gcatggttaa agttatcgat ggctcgctga tattgcccaa gattggcgta cgccacgccc
     5521 atcttgtcaa atattttcgt caaagaattt tcatcgtttg taaaaagcaa cgccagatta
     5581 taatcctgta ttgcacgttg atagtcgccc gtctgattgt aagccatgcc tctgctgatg
     5641 taagcgtttg cataattcgg ttgttgttca atggccttgt cgagatatgc catggcttct
     5701 ttcacttcaa catatttttt cccgtcccat aaaccatccg ctctgttaaa ccagtcagca
     5761 gctgtagacg tttcacccat ggcgagagag ccggaaaata ttatcatcag taaaacgtag
     5821 acgatatttc tgaacatttc agtctgcctc acggtaatct tgcggttttt gtttttcagg
     5881 aaaagaatca aacattgtgc gacgtaagcg ttcctcctct attgtgttac aggcaaatct
     5941 cattttcata tttatgtgcc aaatgattct tacaattgca caccgacccg tgtcaaattc
     6001 aaatcctcct tggccacaaa aaagaatcga ccggtagaat atctgatcgg ccaccttcta
     6061 aatttgtgga acgtttttca ttttcgccaa tccagccaca taatataaat aatcatgaaa
     6121 atgctgtata ttgcggaagg aatggccgct tccttctcaa aaagcgagat ggctaatcct
     6181 cccgcaatgc cctgattttt cagcgttccc aaaagaacaa ggctgaccat attttttctg
     6241 tcgatcttgc aaaaagcaca aattctttga atgaccgagc ccaaaacaaa cgttgtacaa
     6301 aaagaaatga ccgctacgga aaagaccgta aaaggattac tcaaaatcaa atcacggttg
     6361 acaccgatca tggaatatag aacaataaaa aaaccccagt cggtcataaa accacgaaca
     6421 gaagagagtt tctcctgcca attgaaatag agaatcagcc gggacagtat cagcggtaaa
     6481 acgatgagca gaagcataat ttgcagaagc ttatagggat cttcaaacac ggtcccgata
     6541 aatatccaga aaacgaccgg catgatcagc aaagcggcaa tgtaagatgc aacggtgccg
     6601 gacagtgtgt aatcaacatt gccctccaga atggccgtga acggaataac cgccaccgca
     6661 ggcggcacgg acgcaatgag gacaaagcca atccacagat ttttatcttc aatgaacaaa
     6721 gccgataaac acaacaggac accggccaga atgatataac tcataaacat tccggcaagg
     6781 gaaggtttca gtatggcacg cggattcaga aagtagttat tgggaacatt gaccgttgcc
     6841 agcgtcatca ccaatgccag ggcgggaatc atcaaaactt tggtccattc cgccggctgc
     6901 ggcaaaaaaa gacccaggat catcgcaaga ataaaaataa aattacggtt attcagaagt
     6961 ttcatcgcca ttagtatatg atgtcctctc cggattcgac aagatacata aggctgtcgc
     7021 ctgttctgtg caaaagggaa agtttcatag cattgccggg taaaaagacc cttccatgca
     7081 atagtccgct tgacgtctgt gtcctataaa taatttcgga aatatgtccc ggaaaattga
     7141 acaaaacgga aaagatatgg ggcggttcaa cgcgccactg tcaaacaaga caacgccccc
     7201 cctcttgccg tttgtttttt cagctgttca aattaagttc ccagactcac ttcatccgtg
     7261 ccatcgacgg tccacttccg gaaaatattt ttcaaccatt tctttatccc ccacgccggt
     7321 tttccaccga aagaatagaa tcggcataca ttatctttat gtcgaggcag aaccttcttc
     7381 ggtactttgc gttttctcat cttcctgttt catggcttcc cgctttgctc gttccttatt
     7441 aagctcctgc agcaggtcat tggtataatg cagcaatcgt tccgcttcgc tttccagttt
     7501 tcgcattccc ttttccaggg catcgccttc cagttcaatg tcgattaagt catcatcaat
     7561 ttcttcattc attatgacta ctccctttca caatccggtg gatcatcatt cgctctcatc
     7621 agccgttcca cgtggcccga aaatgatatt ctttccataa aatatttccg attctgaaga
     7681 atttttttat gcacatttca tcctcttaaa acgttaatgc cctcatggaa acactgtatc
     7741 ccataccagt tttctacgat gttttcaccg aatcttttct ggggatcgaa cgcctaacca
     7801 tccctttttg ctcataggcc agacaaacaa tccgccaacc gccgtcacga cagcataatg
     7861 tcagaacatc aatacctttg cgaatttctc ttcctgccgt caggatgtaa ttggctttga
     7921 tgacggcaat attgccgtgt ctgttaattt ttacatgatg ccatttttca tcaaataatg
     7981 cattttcagc ggcatattcg cgttgctccg ttataccttc ggttatgctg acaaatgaaa
     8041 cgggttttcc cttttgcacc cgcaccatga tcgcccgctt atcaaacaaa tccatccatt
     8101 catccaaacg tttttgcgaa tacagccggg gataaagttc aaccatgctt tcaagactgt
     8161 agggctgccg atcatttcgt ttttgcataa ttccctttct ttcgcgatga cctgttgctc
     8221 tgattttttt gagtgaaaaa tgaaataccc ttattatttt tccagaacat gaacttctcc
     8281 ggctgcgttg acctgccatt ttttattaac cttctgaaga cggaatcgga tcaccagcac
     8341 ttcgtcattc cggactccat cgctctgaat cgcttttaca tatgctgtgg caacggcttg
     8401 cgctgccgga agcgcaagcg tttcaatttc cagatcctcg agttcgaccg aaagattttt
     8461 gtaatagcct cgggccgtca tcaggtatac cggaacatca tgtcggacaa cagctctgga
     8521 aattccgtag ctcggaatat caacctgaca gttttcggcc atcccttcct gaatgcgtct
     8581 gatgcgatgc gccaccgcca gttcgttttc ccctgttgac cattccagtt ctttcgccag
     8641 tgcctgcaaa tgttttttaa ccgctcgggc ctcccaatca acaaaaaata ataaatacac
     8701 cgcaaaagcc gcaacaagaa tcaatcccat tgccagatat tttcttttta ccataacggc
     8761 actcccacga tacggctgac cggcgcctgt ccgaaggtat gcgtttcagc aggcattgcg
     8821 cgattgtctc ccacgacata cacgtgaccc ggtttgacga taataggttc aagattccag
     8881 ttactttttt tcttgacgta gggttcatta atttttttgt cgtccacgaa aagaatccct
     8941 tttctgaatt caacgcgctg ccccgaaacc gccacaacgc gcttcaaaag catgatccgt
     9001 tgtcccgcca tccgcaccgt cacaacatcc ggcggtgccg gtgaggcaaa catataacgc
     9061 agggcaaaac agaaattgaa cgatccggta acataggtcg gcgccatgct ctcgccctga
     9121 atacgaaaag gaatgaaaac atatttaaat agaataaaag ctaccgcagc cacgacggca
     9181 aggcgcagca catagcgagc ggttatgtcc gggaagaaga atcttctgat ggccgttttg
     9241 ttcatgaatc cttgacgctt ttttggaaat ctttcctctt gtttatttct tgacaatata
     9301 aatcacgacg agaatgacca gcacgataaa aattgcgcca tagcctgttt ccgtaacgcc
     9361 gctccatcta aacacatctc cgataatttc cgctcccatg gcatcgaacg cccggataat
     9421 ataatggccg atttcatttg cgactttatc catgttttat cctcccgatt tcctgcaaat
     9481 ccgctcaacc ggcactcgta tcataatcat aaaacgtcgc ctcgcgtatt ttcatcgtta
     9541 tccccgtcat cggccattca taatgccctc ggcggcgatc atgccggaca ttgccgctcc
     9601 cacaataccg cggcttttgc cggtaccgtc ccctgcgacg aagatgcctt tgacgtttgt
     9661 ttccagataa cggtcggtcg ggaaatgcgt atcgaaaaat tttatttccg gcgcgtacat
     9721 tagcgttgac ggatgcaaaa tgcccgggat gattttgtcc agcgatttga gcgattccca
     9781 cagattgtcc atgatgcgtg cgggcatggc cagggtgatg tcgccggggg ttgcgctgca
     9841 ggtcgccctg cagacttcaa aatgccgcgc cgtgctgttg aacgtggaca gcgaacttct
     9901 cctgccttcg cggaaatccc cgatgcgctg gacgatcggc ttgccgcctc cgagcaggaa
     9961 aaattgtttg gcaatactca atccgaattc cgtagtatcc gaataaggtt tggtcagggc
    10021 aacggtgttg atcagggcaa aattggtatt ctttgttttc ttccctgaca gggcatcgcc
    10081 attgaccagt ttaaaatcac cgtagttttc cacccggact cgcccgccgg gattggtaca
    10141 aaacgtcctg accttatcgc cgtggcgtcc cgtcgtaaaa ataaacttag gatcgtaaac
    10201 gataccggtc acaaagtcat agaattttct gttgagctcc aggcggatcc ccacatcgat
    10261 cggaccgaaa ttattccgga tttccagtgc tctggcgata tcgcggaacc agtaggcgcc
    10321 gctgcggccc ggagcggcca aaatgaccgg cgcgtgacag cttattcgtt ttttattgat
    10381 gcggcagaac agtcggaaga agccctcccg ttttttttca acggactcga cctctgtact
    10441 cagtagaaaa tcggtttttt tcagctgtcc ccgcagataa tccaccagag ccctgccgtt
    10501 atccgttccc cagtgccatt gttcgggcgc aataaattgt gcaccgtgtt cggcggccat
    10561 atccgccaga gcccgaagat tatttctgtt atcggtaaag gtaacctgac ggcagcgcgg
    10621 aaatcccgta aatatcctct tgatctccag catcatgccc tccgccgtca cacggtcgat
    10681 ttgcagttca tcgatgtcca gaccgacacg gtaatcaaag ttcagtttgc cgtcgttgag
    10741 aagtccgccg gcggggtatg gattgcgatc aatgacggct atgtcatcca ttccgagttt
    10801 ttccagccga agcgcagcga acagccctgc cggaccggaa ccgataatca agattttata
    10861 gtttttaatt tgcatcacat taaagacgaa aagcgtttac accggttata agaatgacgg
    10921 ccttttgacc ccaatacagg catgaccaat cttcgcggac gcttttttct tattttacat
    10981 aattagcgcc ggttatcaag tcatgttatt ttatcaatga taccgggtat tttctccatc
    11041 tacaaatcgg catgtccgct gtttgccaca tgacacagca agagagatct attgtttttt
    11101 tcatgcggga tgcctataat ataattataa tattcagtca aaggaggtga taagatcagc
    11161 acaaaaagaa gcagttggtg tgagtcactt atctgttttg gtgatcatat gtgaaaggag
    11221 ggatggtcat gtttgcacca aaaagaattc tcgtaccgac agatttctcc gattactccg
    11281 atgaagcctt gaaacaggcg ttggaactcg caaaacaata tcactcgaaa gtttatctgc
    11341 ttcacgtcgc aaatccggta acacaatgca cagccgatta ttgtctggat gtggatacgt
    11401 tcaagaaagc cgaagaaagc acaatgatta aagcaaggga tatgatgcaa caagagctgc
    11461 agaaattccc tgaatcccgg gagatcgaaa ttatcagtga tatcagagag ggagaacctg
    11521 tggcagagat tctgaaagaa caggaagaaa aaggggtgga cttaatcgtg atgccgtcac
    11581 acggcagatc aggactcttg aagaggctca tgggtcagat tgccgagaag gtcatggagg
    11641 caaccaagac ccccgttctg cttgtgcatc actgatgggc tcgaaaaatc aagaagcaat
    11701 gacacaaggc ggtgttgatt caacaccgcc ttgatcttgt tcagagcatc ccttttttca
    11761 ggatggcgat cagaagccag ccgcccagaa cagcggcggc ggaaaacccg atcaaaccga
    11821 tgagggatat tccatacacc agcggcgggg tgctgtaggc gagaaggtac gcggaaccga
    11881 tgatcagggc ggcaataata atggaaaagg acaggcggtt gctggcctga tcaagatcgc
    11941 gcaaaaaggt atcgatccat cgcatttcca tattgatggt cattttgttc tgacgaatca
    12001 gtctggtcac ctgcaacact tcccggggaa gggtctgaat catatgcatg gattcggaca
    12061 caacggtcaa taaatccgcg gcgatttttt ccggcgcata tcgggccagt ttcgcccgtt
    12121 taataaaagg ctcggcgtag gcaatcatat cgaattccgg atcgagcagg cgggcaatgc
    12181 cctccatcgc ggcgaaggct ttcatcatta aaaatataac cggaggaatt ctgattttat
    12241 tgctggtggc aatatcgagc aaatcctgga tcaaacgggc cagatcaatg tctttgagcg
    12301 atttgaaaag atgcctgccc ataaaatcat caatctctct ttccagcagg cggatatccg
    12361 gttcatcatc gtattctcca agctccagca tcagccgcgc acatttttcc gcatcttttc
    12421 tgccgagcgt ttccataata tccacgaaga gtgcgcgttg ccgtttgtcc acggaaccgg
    12481 tcatgccgaa atcgagaaca caaatgacgt tgttcggcag gacaaagaca ttacccggat
    12541 gcggatcggc atggaaaaac ccgaattcaa aaacctgttt tataacgaaa tgagcgccgc
    12601 gggaggtgat ttttttaaga tccatgccgg cgttttcaag tttttccata tccgacacct
    12661 tgatgccgtc cacatattcc atggtgagaa cccgcctgcc ggacagttct gaaaagactt
    12721 tgggaatgta gatggttctg tcgctgagga actgtccggc aacacgttcc atgctggaag
    12781 cttcagtggt aaaatccagc tctctttcca atgttcgggc aaattcttcg acgacacggg
    12841 tgggcttctg aaatgatatt tcttcgatgt ttttctccat cagcgacgcc agatggagca
    12901 tgatttccag atcgacttcg atgatttttt tgagtcccgg ccgctggatt ttaacggcaa
    12961 caacctcgcc gtcattgagc acggccttat gcacctgcgc gatggaggcg gaagccaaag
    13021 gcttctcatc aaacgacgag aaaaaattat ccagatcacc gccgagctcg gattcgataa
    13081 ttgctttcgc ctcggaaaag gggaaaggcg gcacgttatc ctggagtttt tcgaattccc
    13141 ggataacatc cgcaggaaca aggtccggac gcgtggaaag aatctgggcg aatttgataa
    13201 atgtcggacc gagttcttca aaaagcagcc gcacccgttc cggacggctt aatctttcaa
    13261 cattttcgct ctgatgacgg gaaatcatct gaagaccgat ctcgatatac tgatcgattt
    13321 tcagtctttc caccagatca tcaaatccat atctgaaaag aatggagagg atttgtcgat
    13381 agcggctgaa attccggtac gtccgaccca gcacacctat ttttttgatg cttaacattc
    13441 cgctgaaatc ctcttgcacg ttctgaaacc gtttgtgccg ggcaacagat ccaggatacc
    13501 cggacttatt tcttgcgggc ttttttcaaa ttgccgatct ctttcttaag ggcattgagt
    13561 tctttcctgc tggcaatatc cgcgccggcc atgtagtcgg cgatgccctt ttgaatggtt
    13621 ttttccatgt cacgtctgga ctgctcgtat ttctttgtca aatcggccac aaattttctg
    13681 ccttctgctt cgctcatctt gctttttttg atgatgtctt tggccacggc ttccatttcc
    13741 gagcgggttt tcagagcaag gccgattcct gtcaacataa cttttttcat catttcaaac
    13801 ggcataatgt cctccttgtt gaatggttga tggtcatttt actttaaacc gcttacgatg
    13861 attcacatgt gtcccgttga tcattgatta acgtcttttt gaatataagg aagttacttt
    13921 tacgtgtcaa ttgatttctg aataatgatc gcctcttcaa acggtaaaac actcttcaac
    13981 tcactccggc acattttccg tcatgcgcaa ttgactcagt gcgtattcaa cacatcgatt
    14041 ccggtgatgt aagcttccat aaaatgaact ttcataaagt gggaactgtt ataccgcctt
    14101 cccgtgaact gctgatattt ttcataaaaa cccggcaggt gacgcggtga aaatctcact
    14161 tccttgccga catgtttgaa ccccagcaat cgatacggcg gttgtgcaat cggtccgcct
    14221 tgagccttct caactctgac atcggcaaat ctcggtgccg gtatgggatc ttcggtattg
    14281 acaaaacgga ataacgcgag agaatcgctc ttccttttgc ttttttttgt ccgaagacaa
    14341 gattcatgtc catagcgtgc cgcaaaatac cggttgccaa tgggcggagc gccaaatgta
    14401 tgggaaagaa ttctgttccg ggaaacattg taacagtcgc ataatcgcgc ggcataaagc
    14461 gttgccatgg ctccgcccag gctatgtccg atgatccaga agaaacattt ctttctttta
    14521 agttcatctc ccagcatctg aagcaggttg cctttcagcg gaccgtcctt gaaaataacg
    14581 cgtctgctcc agtcttcaaa atcgtgaacc ctttcaaaaa ttccattgtg aaccttcagg
    14641 cggctgagat gaggaaattt ttctttattc agaaaacgcg tcattcgaat atccagattc
    14701 tggagccagt cctggacctg gtccgaccct ctgaacacga tgacaaccag cgctttggct
    14761 ccttccgggg tctgaatatc gcggcacccg atcagcgcct gtaaatggtt gtggtaaaat
    14821 aactggacgt cgaagaaacc aagtgtcagc agcgtttccg cataacgcgg atcgttgtaa
    14881 taactgtccc gggcaagata agccgcaatc cctgtttttt gcgcatcaag acggccgagt
    14941 ctttccgcag caaacatcat gttccagtcc aggtatccat gatagacatc gtccagcatg
    15001 atggtgtgtg tctcccgtct ttcaaaacat cccattaaat tcttttcatc gaagccgttt
    15061 ttttatcaag atcatgatgg gaaagatatt tttgatgctt accggttcag cttccattta
    15121 atctgaaatt ccacttcctc ttctgaggtg ccgcgttcat gttcgatgtt aatgacggca
    15181 tcggttggaa ttgtaatttt ttctcctgca atctgtatac ggaagggacg accggtttcg
    15241 atacagtcag ccagccgtct cagtttcatg acaaactgcc cggcggtata atctttttcg
    15301 atatctcttt tcttcttttt ctgtttctgc ggcatctgat ttttaccttt ccattgaata
    15361 attttgatcc tttgagaccc tcctcaaacg gcccggccct tcagcggctt atcttacaga
    15421 aaactgattt ccttggtcga cgccgtcacg acataacaac gttcattatt ggatccggca
    15481 tccgccggca caaggaagaa tcccggacgt ccgggttgat agcctgtcgt ggtgccgatc
    15541 aggacttccc cgtccttgaa aaccactttg atcttgcgcc cgacaaccgg ttgcgatgca
    15601 ttaaactcgt tggttttgac gtgctgcgga ttgcc
//
LOCUS       DAOOLB010000043        15582 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4010_length_15582_cov_37.222129, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000043 DAOOLB010000000
VERSION     DAOOLB010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4010_length_15582_cov_37.222129"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4408)
                     /locus_tag="PLF77_06760"
     CDS             complement(<1..4408)
                     /locus_tag="PLF77_06760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC08589.1"
                     /translation="MKKKSKTSQKSILINKKTSSKTKSSSKSSPALKKEASNSSKCKP
                     VKIESKKSFSLDRDIHEVIAREWQTAFDAVTDAICLLDKNQRILRSNRAMQDLFAVSG
                     KKIVGKYCWEIIHGTAKPVPGCPIVKSSRSRKPEKMELKTKGRTFNVTAYPVFDEKNT
                     AIIGFVHIINDVTELKQAWEIVNASEMRLKAQYQGSPLAIFIWQKQDKDFVLIDFNKM
                     AFALSQGLVKKFINKTARQLYKKRPDILKDIQDCFAKKTTIKKELFSERFIPGTYIKA
                     SYSFVPPDLVMVHIEDISERKQAEAALRESESLYRMLTEKMSDIVWTMDMSMRTTYVS
                     PSVKKVLGFTPKERLRQTVEEQLTPESLAAAVNIMDRELSDEKQGKSNPEKNVTLTLE
                     YYHKNGSTRWLETTVSGLRNEQGKLTGLHGLSRDITQRKRMEDELIESEKKYHSIVDN
                     STDAILLTIPDGDVLSANAAACKMFDRTEEDIRRAGRSGLMDLSDPRLPAALEERKQT
                     GRFRGELTFVRKDGSKFEGEISSVIFTDRKGTPLTSMIIRDISERKQAEEALKQRDLL
                     FKKLSAHVPGMIYQFVKKPDGTYCLPFSTDAIRTIFGCFPEDVAHDYSPILHAVHPED
                     VDDFLNSIEYSTQHMTDWKHEYRVQLPDGTVKWMLGQSTPEKLPDGTIVWHGFNADIT
                     DQKNIQESLKATQDNYRRLFEDHSAVKLIMDPETGAILDANFAAANYYGWTREEMKQM
                     KISQINILSPDEIKHEMDKVMQEHKVHFEFRHRRADGSIRDVEVYSSRVRRNGSYVLH
                     SIIHDVTERKQAERELKQAQAYNEAILKSIPGSLYLIDENGRLVHWNKQFEILTGYDA
                     HEIQDSFVLDWFKDRQNDLLNIKAGIDTIMTRGSASNVEANLQTKDGRLIPMLYTGVR
                     FDIGGKHHILGVGTDITERKVAEEKLLREEKRFRSFAEQSSDIIALVDKNGTMLYENP
                     AVEKLLGYKQEERIGQDTFLNVHPDDLEDLMRCFQILFTDKNVPVQKTETRIRHKDGT
                     WLTFEISGSNVVNNDVIEFITVSLHDITERKRIQQELLESRNYAEKLLRTANALIVVL
                     DTEGNVVSLNEAGEKITGYRAGDIAGLNWFDLIVPKNRYPYVWEAFKKWKFSNDLPES
                     MENPIVTKSGEERLISWRNSTIIEKGGFAGILAFGMDVTERKKMEESLQSWMRRYELI
                     VAASGQVAYEYTLSTGRIIWGSSIANVLGYDMEEIGGNFEQWESLLHPDDRDATIQSL
                     EAAEKACGFWESQYRMKHKKGHYVWIRDRGFFVPDAEGKAYYQLGMMEDITSLKQAEE
                     ELKRTLLMNKAILESVPGILYLYNDTGHLVQWNRQFEVLTGYSGDELQGKYVMDWFGG
                     LEPDTSNIKKGISDVMTKGYSSAEARMITKNGDHLFMFFTGVKLTVSGQDYLLGIGLD
                     ISERKKAEMEIVRLNESLEEKVQERTRELEAFS"
     gene            complement(4586..7096)
                     /locus_tag="PLF77_06765"
     CDS             complement(4586..7096)
                     /locus_tag="PLF77_06765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HPC08590.1"
                     /translation="MRRPLILLLIAMIAGILAGSYWIIPFYSLCAAAVVTLILILVSL
                     RKKWLPAGFVFIFLVVFLLGFFNIQKAFHFPEHRPHILSYANQGKITVEGFVSESPVA
                     YADKNVLIVQPLRILKDKTYRPVFGAVRLSIPSGLTFYLGDFIRFQSSLRKIENFNNP
                     GALNYKRLMNLQGIYISGYIHDASDIILLRKQPSFGVHRMIESFRLYLKRIIGNHAPM
                     PEKSIIEAMTLGNQNEIPSVIRDNFSKTGTSHILSISGLHVGMVAASFFVLIFLILKS
                     SEYLMLKFNIVKIAAGFSFVIVLLYAVIAGMGVTVMRATFMAFVFLLALIFGRQKELY
                     NTLAIAGLMILAVAPEALFDISFQLSFVSVLAIIYITPRLNGLIQHKNLPLPSWLQSP
                     FRYLYMTIMVCIAATLGTLPLTVFYFDRVSLVTIAANLIAVPLLGTLTLALAMFFILS
                     AFFSEVVAGFFVQLAAFATRISVNIINHLADFTWSSVSLSKPSLMEIALFYLLIFFAV
                     QWIDEIRKKKNRLHFSSSRFAFINYALIFIVVMIGANAAYLTLRERISNDLTVTVIDV
                     GQGNSALVQFPKGTNMMIDGGGFSKSSFDVGKGIVAPFLHKQRIRRIETVVLSHPHPD
                     HLLGLIYILNHFNVSQIWKTALPVNPDIYPEWESAVKTNRIKTLLLGVTAGEKNVNGV
                     RIKILWPPDHVDPGGKALSHNDENDLSMVMKITYGHVSFLFPGDISSKVENILVRSGA
                     DLRSDVLVVPHHGSNHSSSSDFIRAVGCRFAVVSAGRSNVFRHPHPSVLQRYRQAGVY
                     IFRTDCDGAVTFKTDGHHLRTETFFQKTADIPEKQQIR"
     gene            7195..8880
                     /locus_tag="PLF77_06770"
     CDS             7195..8880
                     /locus_tag="PLF77_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08591.1"
                     /translation="MTTSNRDESANGKNKRRILSLIISRIAITTLLLAITVFIDTRRQ
                     LFGIPYAAINVFYFIVVVIYLFSSLYIVLYKLKINYLYNLYLQIAIDIVTVTFLIFMF
                     GNTQIDYSLFYTLIIIYSALFLGRKGGLIVASISSIFYGIFLNLEFYKLMPSQSLIKL
                     DYDMNAADALTNLVVRITSFYVLAFLASFIIEQEKKTSSLLEEKESEFNQLDLLFRSI
                     VESVYSGVMTVNLNNIIKTFNTAAEEITGFPRTRVQGLNIQNIFPEFLPYLNKETIDE
                     QAKARIEIIIQGRKKEKINLGVSVSPLIGKSEKQIGNILIFQNITQIKQMEKALEKNK
                     NMALIGEMAAGWAHEVRNPLAAITGSIELLKQGLNPDGTNKRLMEIVLRGKDQLENFV
                     RNFLMLARTVPAAREMVDINRIIDEIQEALQIDKDWNDTIKIKKILSGDVSVYANKEQ
                     IRQVLNNIINNAVEAMEEGGLLSIGTRVVQTSGKKKYAEIVVSDTGCGIKEEDLNKIF
                     EPFFTKKTRGTGLGLAIVNHLVEGYKGKIEIESTEGKGTRCRLLLPVEKDENL"
     gene            8922..10280
                     /locus_tag="PLF77_06775"
     CDS             8922..10280
                     /locus_tag="PLF77_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC08592.1"
                     /translation="MAKILIVDDDQGMREMLEIMLQKEGYDVTSIGDPVKAVDLCRKK
                     NFDMVITDLKMPKIDGIEFLKSVKDQRPETIVILITAYASGETAINAMKEGAYDYVEK
                     GRSLDELKKVVRAALMNKGLISDDPAEQKCTGATESNHAFCGMIGTSREMLKIFNTIK
                     KVSDTPANILILGESGTGKELVARAIHENSSRSKMPFVAINSGGIPENLLESELFGYM
                     KGSFTGAYADRQGYFEMARGGTIFLDEIGELSPSLQVKLLRVVQEKTFRRIGGAEDIK
                     VDVRIISATNKNLQERVKKGDFREDLYYRLNVIPILIPPLRERREDIPLLIDTFIEKY
                     SKMFGKEIRTISSYAVELLLNYDFPGNIRELQNIIERGVAMESSNIILPESLTFGAPK
                     FDVDISDQGIDLNAELDKIEKTIIEKALQKTKGSKKKAAELLNVTFDSLRHRIEKLGV
                     ES"
     gene            10570..11772
                     /locus_tag="PLF77_06780"
     CDS             10570..11772
                     /locus_tag="PLF77_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPC08593.1"
                     /translation="MKSRILIAILSAVFLIAGTGSLQAKTIKVGAAINLTGPASTWGQ
                     FHEKGQRDYFRYVNEVKGGVYGNKIEMITVDTAYKVPEAQAAVNKFVLQDKVDMIATW
                     GAGEGLAAKPIIQRYKVPAINYSTSWEILEKPVDYMYLPFGSYKMDCHAVFEYIKSIH
                     KGKDAPKVGLLTYNNAYGRSIHAPSKAYAKQLGINLVAVEEFPPKTVDLNTELLRLQK
                     AGAQYIFMQMLPSAIITAFKSADRINYNPVFLGTWTSTDPDFFKMGKGLIRDRLVIQF
                     PGGLPSDKSKGMAVMQELWKRYKTVSKFDASYWEGVVVGMIMERAFMRAYEKSKNINP
                     ATINAAMESFRNEDFGGLVPAITYTKDNHGASFTARMVKVKEDGTYIPLTNFYVPGRD
                     KIKLLGSK"
     gene            11842..12669
                     /locus_tag="PLF77_06785"
     CDS             11842..12669
                     /locus_tag="PLF77_06785"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC08594.1"
                     /translation="MKAFRSRPSIMINRREENKKAELAIQNLRLSFGGVVAVKDVDLQ
                     VHTGELMAIIGPNGAGKTSLLNCITGFYRAQQGKIVFNGKEITRLHTHELVGVGIGRT
                     FQNIELFPGMTVLANMTLARHIHCRYSFIKSFLFSKSVRDEEIRHRQVLEEIIDFLEM
                     QSIRKQAVGSLPYGMQKRVELGRALALEPKLLVLDEPFAGMNMEEKEDMVRFLLELNQ
                     QWGLTMILVEHDMSIVMSISQRIMVLNFGEKLGEGNAEEISANPEVIKAYLGESEAM"
     gene            12691..14616
                     /locus_tag="PLF77_06790"
     CDS             12691..14616
                     /locus_tag="PLF77_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPC08595.1"
                     /translation="MSKGTSHITKRNLTLPQVLMENAAVYGDAKTAIREKAYGIWQKY
                     TWADYGRYVRMTAAGLAALNLRRGENLCMVVHNHPEWLFSELAAHVLGATTLNLFTSS
                     IADELAFSIKRINSPVVVVQDQEQVDKLLEIKEKLPQTQKVVYIDPTGMASYQDDPWL
                     ISYAGLLKLGEQYLEEHPGFIDSEINRGRPDDIAVMIQTSGTTGVPKLAMLSHRNLLT
                     MARQWIEETRIKPEENWLSMSPPAWIVDQMWGMGVALDGAMCMNFPETAETVLEDFRE
                     IGPSMIITSSRFWEDMASRIRVKISDAGWLKKTLFYLGEKIGKSVVEKQSHRQPVGWP
                     LQVLHSVMSMIVYRPLLDRLGCSHFRSAFTGGHPISPDVIHFFRAIGLNLKQCYGLTE
                     SCGIFQIQPDHEVKLETVGKPMVLTEVKIAGDQEVLVKSEANFSGYHNDYESTQNAFE
                     NGWLKTGDAGYIDHDGHLLIIGRKEDIIRNKSGEAFSPDFIETRLKFSPYIKEAVTFG
                     EARPYIAAMINIDMGNVGNWAEERMIPYTTYMDLSQQSAVEELILKEVIAVNEQLPEV
                     MKIKKFVLLYKLLDADDEELTRTGKVRRRFVYGLYLKIIEAMYNNLKNVQVTGKVKYR
                     DGRIGEIETTINVINVN"
     gene            14631..15509
                     /locus_tag="PLF77_06795"
     CDS             14631..15509
                     /locus_tag="PLF77_06795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPC08596.1"
                     /translation="MEFFLQLLVNGVCIGLLYGISAMGFVMIFKSSSVLNFAHGELLA
                     LGAFFFLSLVTWAQLPVYVAFILTMIGCFILGFLIERFFLRPLIGEKLIFVIMLTVGL
                     AAMFKGFILLVWGGNLHTYPEFLPPSMAMQFGSIYVAPAYSATLMISVVFLVLFGLFF
                     KKSSQGIYMRSVADNQKAALSLGVHVRRVFALSWAIAALVACMSGIVLGIINGVNVHD
                     LSSIGLKVFPVVILGGLDSIGGAIIGGIIIGLLETFTGGYLSPSLRDVVPYIVLVFIL
                     MVKPYGLFGLVEIERV"
BASE COUNT         4067 a         3479 c         3906 g         4130 t
ORIGIN      
        1 aagaaaacgc ctccagctct ctggtgcgct cctgaacctt ttcttccagg gattcgttca
       61 gcctgacaat ttccatctct gcttttttgc gttcgctgat atctaagcca atgccgagaa
      121 gataatcctg gcccgagacg gtaagtttga cgccggtaaa aaacataaaa agatgatccc
      181 cgtttttagt gatcatacgt gcctcggcgg aagaatatcc cttggtcata acgtcggata
      241 tgcctttttt aatgtttgat gtatcgggtt ccaggccgcc gaaccaatcc ataacatatt
      301 ttccctgtag ttcatctccg gaatagcctg ttaacacttc aaactgcctg ttccactgca
      361 ccagatgtcc ggtgtcgtta tagagataaa gaatgccggg aacgctttcg agaatggctt
      421 tgttcattaa cagggtccgt ttcaattctt cttcggcctg tttaagggag gtgatgtctt
      481 ccatcatacc gagttggtag tacgccttgc cttcggcatc cgggacaaag aaaccgcgat
      541 ctctgatcca cacataatgt ccctttttgt gtttcatgcg gtattgcgac tcccagaagc
      601 cgcaggcttt ttcggctgcc tctaaagatt gaatcgttgc atccctgtcg tcaggatgca
      661 ggaggctttc ccattgttcg aaattgccgc cgatttcttc catatcgtat ccgaggacgt
      721 ttgcgatact ggagccccag ataatccggc cggtggataa agtatattca taagccactt
      781 gtccggaggc cgccacaatc agttcatacc gccgcatcca tgattgcagg gattcctcca
      841 tcttcttgcg ttccgttaca tccatcccga acgctaagat gccggcaaac ccgccttttt
      901 caataatcgt gctgtttctc catgaaatca ggcgttcttc gcccgatttt gtcacaatgg
      961 gattttccat ggattccggc aaatcgttgc tgaatttcca ttttttgaat gcctcccaga
     1021 catagggata acggtttttc ggaacgatca gatcaaacca gtttaaaccg gcaatgtctc
     1081 cggcccggta accggtaatc ttttctccgg cttcgttcaa tgaaacgacg ttgccttcgg
     1141 tatcgagaac cacaatcaat gcattggccg ttctgagcag tttttcagcg taatttctcg
     1201 actccagtaa ctcctgttga attctttttc gctcggtgat gtcatgaagg ctgacggtaa
     1261 taaattcgat aacatcattg ttgacgacat tacttcccga tatttcaaag gtgagccagg
     1321 ttccgtcttt atggcggatg cgagtttccg ttttttgaac gggcacattt ttatccgtaa
     1381 acagaatctg gaagcatctc attaaatcct ccagatcatc agggtgtacg ttaagaaacg
     1441 tatcctggcc gattcgctcc tcctgtttgt accccaggag tttttccacg gcgggatttt
     1501 cataaagcat tgtgccgttt ttatccacca gcgcaataat gtccgaggat tgctcggcaa
     1561 atgaacggaa acgcttttct tcccggagca gtttttcttc tgcaactttt ctttcggtga
     1621 tgtcggtacc cacacccagg atatgatgtt taccgccgat atcgaatctg acccctgtgt
     1681 aaagcatggg aatcaatcgt ccgtctttcg tttgaaggtt ggcctccacg ttggaagccg
     1741 atccacgggt catgatggta tcgatgcccg ccttgatgtt taataaatcg ttttgccggt
     1801 ccttaaacca gtccagaaca aaactgtctt ggatttcatg ggcgtcatag ccggtcagga
     1861 tttcgaattg tttgttccag tgtaccagac gtccgttttc atcgatcaga taaagactcc
     1921 ccggaatgct tttgagaatg gcttcgttat aggcttgagc ctgttttaat tctctttccg
     1981 cctgtttgcg ttcggttacg tcgtgaataa tagaatgcaa aacgtaagag ccattccttc
     2041 tgaccctgct ggaatatacc tcgacgtctc tgatggaccc gtcggcgcgg cgatgacgga
     2101 attcaaaatg gaccttatgt tcctgcataa ccttatccat ctcgtgcttg atttcgtcag
     2161 gggaaagaat attgatttgc gagattttca tctgcttcat ttcttcccgc gtccagccgt
     2221 aatagttggc tgcagcgaaa tttgcatcca gaatcgctcc tgtttcggga tccataatga
     2281 gtttgaccgc cgaatggtct tcaaagagtc tgcgataatt atcctgcgtt gccttgagac
     2341 tttcctgaat gtttttttga tcggtgatat ccgcattgaa cccgtgccag acaatggtgc
     2401 cgtcgggcag cttttcaggt gttgattggc ccagcatcca tttaacagtg ccgtcgggca
     2461 actgcacccg gtattcgtgc ttccagtcgg tcatatgttg agtggaatat tcgatggagt
     2521 ttaaaaaatc gtcaacgtct tccggatgta cggcatgtaa gatcggagaa taatcatgag
     2581 cgacatcttc cggaaaacag ccgaaaatag ttctaattgc atcggtggaa aacggcaggc
     2641 aatacgtccc gtccggtttt tttacaaact ggtagatcat tccgggcaca tgggcggata
     2701 attttttgaa taaaagatcg cgctgtttca gggcctcttc cgcctgcttg cgctcggaaa
     2761 tatcgcggat gatcatactc gtcaaggggg tgcctttgcg atccgtgaaa atcacggagg
     2821 aaatttctcc ctcgaatttc gatccgtctt tgcgcacaaa ggtcaattca cccctaaacc
     2881 ggccggtttg cttgcgttct tcaagagcgg ccggcaaacg cggatcactc agatccatca
     2941 ggccgcttct gccggctcgg cggatatctt cttcggtgcg gtcgaacatt ttgcaggcag
     3001 cggcattggc cgaaaggaca tctccgtcgg ggatggtcag caaaatggca tccgtgctgt
     3061 tgtcgacaat ggaatggtat ttcttttcgc tttctattaa ctcatcctcc atgcgtttgc
     3121 gttgcgtgat gtctctcgac aaaccatgaa gaccggtcag tttcccctgc tcatttcgaa
     3181 gcccgctgac ggttgtttcc agccagcggg tcgagccgtt tttgtgataa tattcaagag
     3241 tcagcgtgac gtttttttcc ggattacttt tcccctgttt ttcatcagaa agttcacgat
     3301 ccatgatgtt tacggctgcc gctaaggatt caggcgtcag ttgctcttca accgtctggc
     3361 gcaatctttc tttcggggtg aagccgagca ctttcttgac ggacgggctg acatacgttg
     3421 ttctcatgct catatccatc gtccagacaa tgtcgctcat tttttccgtg agcattctgt
     3481 acagactttc gctttcgcgc aatgcagctt ctgcctgttt tcgctcggag atgtcttcaa
     3541 tatggaccat caccagatcg gggggcacaa aactgtaaga ggccttgata taggttccgg
     3601 gaataaagcg ttccgagaat aattcttttt tgatggttgt ttttttcgca aagcagtcct
     3661 gtatgtcctt taaaatatcc ggtcgttttt tgtataactg gcgcgctgtc ttgttgataa
     3721 attttttaac cagaccctgg gagagggcga aggccatttt attgaaatcg atcaggacga
     3781 agtctttatc ctgtttctgc cagatgaata ttgccagggg gcttccctga tattgcgctt
     3841 taagccgcat ttcactggca ttgacaattt cccaggcctg tttgagttcg gtaacgtcgt
     3901 taataatatg gacaaagccg atgatggcgg tatttttttc atcgaaaaca ggataagcgg
     3961 tgacgttaaa ggttcgtcct ttggtcttaa gctccatctt ttccggtttc cgggacctcg
     4021 acgatttgac gatcgggcat cccggaaccg gttttgccgt cccgtgaata atttcccagc
     4081 agtatttccc gacgattttt tttccggata cggcaaacag gtcctgcatg gcccgattgg
     4141 aacgaaggat acgctggttt ttgtcaagca gacagatggc atcggtgacc gcatcaaagg
     4201 ccgtttgcca ttcacgggcg atgacttcat gaatatcccg gtcgagagaa aaagattttt
     4261 tcgattctat ttttaccggt ttgcattttg aggagtttga tgcctccttt tttaaagccg
     4321 gcgaagattt ggatgaggat tttgttttgc tgctcgtttt tttgtttata aggatgcttt
     4381 tttgtgaggt ctttgatttt tttttcatgg cgtaatatcc cttttgtcga ttgtgtaatg
     4441 tgaaggtttt tcagcaaaga acgccgccgg cacaatgtga tcaaagaacg ggtttcctca
     4501 aggtcctaaa aaagaaagca ctaacaaaaa acgggataag tacatcattt acttgaaaat
     4561 aattataggc tgataattta taaagtcaac ggatttgttg tttttcggga atgtcggcag
     4621 tcttttgaaa aaaggtttcc gtgcgcagat gatgtccgtc agttttaaag gttaccgcac
     4681 cgtcgcaatc ggtgcgaaaa atatacaccc cggcttgccg gtatctctgc aaaacggaag
     4741 gatgaggatg cctgaagacg ttcgatcgtc ccgcgctgac aacggcaaaa cggcagccga
     4801 cggcccggat gaaatcgctg ctgctggaat gattggatcc gtgatgcggc acaaccagga
     4861 catcgcttct taaatcggcg ccggagcgga caagaatatt ttccaccttc gaagatatgt
     4921 cgccggggaa caaaaaactg acatggccgt aagttatttt catcaccatc gataaatcgt
     4981 tttcgtcgtt atgggaaagc gccttcccgc ctggatcgac atgatcgggg ggccagagaa
     5041 ttttaatccg gacgccgttt acgtttttct cgccggcggt tactccaagc aaaagggttt
     5101 tgatccggtt tgttttaacg gcgctctccc attccgggta gatgtccgga tttacgggta
     5161 atgccgtttt ccagatttgt gagacgttga agtggttcag aatataaatc agtcccagca
     5221 ggtgatcggg atgcggatgg ctcagaacga ccgtttcaat gcgccgtatt ctttgcttgt
     5281 gcagaaaagg tgcgacaatg ccttttccaa cgtcgaaaga acttttggaa aaaccgccgc
     5341 cgtcaatcat catgtttgta cctttcggaa actgcacaag tgcggagttg ccctgtccga
     5401 cgtcgatgac cgttacggtc aggtcattgg agatcctctc gcggagtgtc agataagctg
     5461 cgttggcgcc gatcatgaca acaatgaaaa tcaaggcata attgatgaag gcaaagcggc
     5521 tggatgaaaa gtgtaaacga ttttttttct tcctgatttc gtcaatccat tgcaccgcaa
     5581 aaaaaatcaa taagtaaaat aaggcgattt ccatcagcga cggtttagac agggaaaccg
     5641 acgaccaggt aaaatcggca agatgattga tgatgttgac tgaaatccgg gtggcgaagg
     5701 ccgccagttg gacaaagaat ccggcaacga cctcggaaaa aaaggcgctc agaataaaaa
     5761 acatggccaa ggcgagggtc agggtgccca gaagaggaac ggcaataaga ttagccgcaa
     5821 tcgtcaccag cgacacacgg tcgaaataaa atacggtcag aggaagcgtg cccagtgtgg
     5881 cggcaataca gaccataatg gtcatgtaga gataccggaa gggactttgc agccatgacg
     5941 gaagcgggag gtttttatgc tgaatcaatc cgttcagtcg cggcgtgata tagatgatgg
     6001 ccagaacgga aacgaaggaa agctgaaatg aaatatcaaa aagcgcttcg ggcgcaaccg
     6061 ccagaatcat cagaccggca atcgccaagg tattgtaaag ctctttttgc cttccgaaga
     6121 tcagcgcgag cagaaaaacg aatgccatga acgtcgcccg cattaccgta acccccatgc
     6181 ccgcgataac ggcatacagc aggacgatca cgaaagaaaa tcctgccgca attttgacga
     6241 tgttgaattt gagcatcagg tattccgatg attttaaaat cagaaaaatc agcacaaaaa
     6301 aagaagcggc caccatgccg acgtgcaggc cggaaatgga aagaatatgc gacgttccgg
     6361 tcttgctgaa attgtccctg ataacggacg ggatttcatt ttgattgccg agcgtcatcg
     6421 cttcgataat gcttttttcc ggcatcgggg catggttgcc gatgatgcgt ttgagataaa
     6481 gccggaagga ttcgatcatt ctatgaacgc cgaacgaagg ttgttttctc aacagaatga
     6541 tatcagaggc gtcgtggata tatccggaaa tataaatgcc ctgcaggttc atcaggcgct
     6601 tgtagttcaa cgctcccgga ttgttgaaat tctctatttt ccttaaagac gactgaaaac
     6661 ggatgaagtc tcctaagtaa aaggtcaggc cggagggaat ggacagacgg acagccccga
     6721 agaccggacg atatgtttta tcttttagaa tgcgcagagg ttgcacgatg agaacatttt
     6781 tatcggcata ggcgacggga ctttcagaaa caaagccttc aacggtgatt tttccctggt
     6841 tcgcataaga aagaatgtgg ggccggtgtt cgggaaagtg aaacgccttt tggatgttga
     6901 aaaagcccaa aagaaagacg accagaaaga taaaaacaaa tccggccggc agccattttt
     6961 tccggagact gaccaggata aggattaggg tgacaacggc agcggcgcac aagctgtaaa
     7021 acggaatgat ccaataactg ccggcaagga tgcccgcaat catggcgatc aagagaagaa
     7081 tcaacggtcg gcgcataatc gtttttgact ttcaaccgca tgcatgaaat atattagcga
     7141 aaagtgctaa aatattataa tatatttcgc aaatgttttt tttgaggaat ctgcatgacg
     7201 acatcgaata gggacgagtc cgcaaacggg aagaataaac gcaggatttt atcgttaatc
     7261 atctccagga tagcgattac cacgcttctg cttgcaatta ctgtttttat cgatacccgt
     7321 aggcagttgt tcggcatccc ctatgccgcg attaacgttt tttacttcat cgttgttgtt
     7381 atttatttat tttcctctct gtacatcgtt ttgtacaaac tcaagatcaa ttacctctat
     7441 aatttatacc tgcagatagc gatcgatatt gttaccgtta cgtttctgat tttcatgttc
     7501 ggcaacaccc agattgatta ctcccttttt tatacgctga tcattattta ttcagcgctc
     7561 tttctgggac ggaaaggagg cctgattgtc gcctccattt ccagtatatt ttacgggata
     7621 tttctgaacc tcgagtttta taaattgatg ccgagccagt ctctcattaa actggattac
     7681 gacatgaacg cggccgatgc gctgaccaac cttgtcgtgc gcatcacttc tttttatgtt
     7741 ctggcttttc tggccagctt cattatcgaa caggaaaaaa agacctccag cctgctggag
     7801 gaaaaggaat cggaattcaa tcaactggat ttgcttttcc gaagcattgt ggaatcggtt
     7861 tacagcgggg tcatgacggt gaatctgaat aacatcatca aaacattcaa tacggcggct
     7921 gaagaaatca ccgggtttcc ccgcaccagg gttcaggggc tcaatattca aaacatcttt
     7981 cccgaatttc tgccctatct gaacaaggaa accattgacg aacaagccaa ggcgagaatt
     8041 gaaatcatta ttcaaggcag aaaaaaagaa aaaatcaatc tgggtgtgtc cgtatccccg
     8101 ctgatcggaa aatccgaaaa acagataggc aatatcctga ttttccaaaa tattacgcaa
     8161 atcaagcaaa tggaaaaggc tctggagaaa aacaaaaata tggctttaat cggggagatg
     8221 gccgcaggct gggcgcatga ggtcagaaat ccgctggcgg cgattaccgg ttcaattgag
     8281 ctgttgaagc agggactgaa tccggacgga accaataagc gcctcatgga aattgtcctg
     8341 cggggcaagg atcaactgga aaattttgtg cggaattttt tgatgctggc caggaccgtt
     8401 ccggcagccc gggaaatggt ggatatcaac cggatcatcg atgagattca ggaagctctc
     8461 cagattgaca aggactggaa cgacacaata aaaatcaaaa aaatattatc cggagacgtc
     8521 agcgtttatg ccaacaaaga gcaaataaga caagttttga ataacatcat caacaatgca
     8581 gtcgaggcca tggaagaggg cggtttgctg tccattggaa cccgagtggt ccagacgtcg
     8641 ggtaagaaaa aatatgcgga aattgtcgta tccgataccg gatgcggtat caaagaagag
     8701 gatttaaata aaatattcga gcctttcttt accaaaaaaa caagggggac cggtttaggg
     8761 ctggccatcg tcaatcatct ggttgaaggc tataagggaa aaattgaaat agaaagcacg
     8821 gaaggcaaag gaacccggtg ccgtttactg ttgccggttg aaaaggatga aaatttgtga
     8881 tttagaattt gacaatgcgt tgttgagaca atgaggtcga tatggcaaag attttgattg
     8941 ttgatgatga tcagggcatg agggaaatgc ttgaaatcat gctgcagaaa gaagggtatg
     9001 atgtcaccag catcggcgat cccgtgaaag cagtcgattt atgccggaaa aaaaatttcg
     9061 atatggtgat caccgattta aagatgccta agatcgacgg aattgaattt ctcaaaagcg
     9121 tcaaggatca gaggccggag accatcgtta ttctcatcac cgcctatgct tccggagaga
     9181 cggcgatcaa cgcgatgaaa gaaggcgcgt acgattatgt ggaaaaggga cgcagtctcg
     9241 acgaactcaa aaaggttgtg cgtgccgcgt tgatgaataa aggcctgatc agcgacgatc
     9301 ccgcggagca aaaatgcacc ggcgccacgg aatcaaacca tgccttttgc ggcatgatcg
     9361 gcacaagccg ggaaatgctc aaaatattca acacgattaa aaaggtttcc gacacgccgg
     9421 cgaacattct gattctcggg gaaagcggga cgggcaagga actggttgcc cgcgccattc
     9481 atgaaaattc ttcccggtct aaaatgccgt ttgtcgcgat caacagcggc ggcataccgg
     9541 aaaatcttct ggaaagcgag ctcttcggat acatgaaagg gtcttttacg ggagcttatg
     9601 ccgaccggca ggggtatttt gaaatggccc gcggcggaac gatttttctc gatgaaatcg
     9661 gagaactatc gccgtctctg caggtcaaat tgctgcgggt ggttcaggaa aagacattcc
     9721 gtcgcatcgg cggtgcggaa gacattaaag tggatgtgcg cattatttcc gcgaccaata
     9781 aaaatcttca ggaaagggtg aagaaagggg actttcgcga agacctttat tatcgcctga
     9841 acgttatacc gatcttgatc cctccgctga gggaaagacg ggaggatatc ccgctgctga
     9901 ttgatacctt catcgagaaa tactcaaaaa tgttcgggaa agaaatcaga accatttctt
     9961 cgtacgcggt ggagctttta ttgaattacg actttccggg caatatccgc gaactgcaga
    10021 atatcatcga acgcggcgtt gccatggaaa gctccaacat tattctgccg gaaagcctga
    10081 ctttcggagc cccaaaattc gatgtggata tttcagacca gggcattgat ttgaacgcgg
    10141 aactggacaa aatcgagaaa acaatcattg aaaaggcatt gcagaaaaca aagggatcga
    10201 agaagaaagc cgccgagctt ttaaatgtta cgtttgattc cctgcggcat cgtatcgaaa
    10261 aactgggtgt ggaatcgtaa atggtggtat taagaaaaat tataccgaaa ccttcttttt
    10321 gcttgacttt tcaatagcgt ttgctctata tgactccaca ttgggagttt aatgaatatg
    10381 ttaaggattt atttttttaa aaaggcagtc ttttttttct tttttagctt tatacgtaaa
    10441 gtctgcctga tagtatcatg accatccgta agtggtaatg aaccgtgggc agataaaaag
    10501 cccacggttt ttttttgccg tcatcaggac ggccgtttta aaaaaagaaa gaaggggagg
    10561 ggaataaaaa tgaaaagcag aattttaata gcgattttat cagcagtttt tttgatcgcc
    10621 ggtaccggaa gcttgcaggc caaaaccatc aaggtcggag cggcgatcaa tttgaccggt
    10681 ccggcttcaa cctgggggca gtttcacgaa aagggacagc gcgattattt ccgttacgtc
    10741 aacgaggtga aaggcggcgt ttatggaaac aaaattgaaa tgatcacggt ggataccgct
    10801 tacaaagtac cggaagcgca ggccgcggta aataaattcg ttctgcagga caaagtggat
    10861 atgattgcca catggggagc cggcgaaggc ctggcggcta agccgatcat ccagagatac
    10921 aaggttccag ccatcaatta ttccaccagt tgggagattt tggagaaacc cgttgattat
    10981 atgtacctgc cgttcggcag ttacaagatg gattgccatg cggtttttga gtatattaag
    11041 tccattcata aagggaaaga tgctcccaaa gtcggtcttc taacctacaa caacgcctac
    11101 ggccgttcca tccatgcgcc gagcaaagca tacgcgaaac aactgggcat caatctggtc
    11161 gcggttgagg aatttcctcc gaaaacggtt gatctgaata cggaacttct ccgtctgcag
    11221 aaggcgggcg cgcagtatat ttttatgcaa atgcttccgt ccgcaattat cacggcgttc
    11281 aaaagcgccg accgcatcaa ttacaatccg gtattcctgg gcacctggac atcgacggat
    11341 cccgactttt tcaaaatggg caagggactg attcgcgacc ggctggtcat tcagtttccc
    11401 ggaggattgc cttccgacaa gtccaaggga atggccgtca tgcaagaact gtggaaacgg
    11461 tataaaaccg tcagcaaatt tgatgcgtcc tactgggaag gcgttgttgt cggcatgatt
    11521 atggaacgtg cctttatgcg tgcttatgag aaaagtaaaa acattaatcc ggcaacaatc
    11581 aatgcggcga tggaatcatt cagaaacgaa gatttcggcg gtttggttcc ggccattacc
    11641 tatacaaaag acaatcatgg cgcttcgttt actgcgcgga tggttaaagt gaaggaagac
    11701 ggaacgtata ttccgctgac gaatttctac gttcccggca gagataaaat caagctgctg
    11761 ggcagtaaat aaatcatgat ggcaccaggc gtgatcagaa ccttgattct gatcacgccg
    11821 ttttctaaag aagaggtttg aatgaaagcg tttcgcagcc ggccgtcgat tatgatcaac
    11881 cggcgggaag agaacaagaa agccgaactg gctattcaga acctgcgcct gagtttcggc
    11941 ggtgttgttg ccgtcaaaga tgttgatctg caggttcata ccggtgaatt gatggcgatt
    12001 atcggtccca acggagcggg gaaaacgagc ctgctcaact gcatcaccgg tttttaccgc
    12061 gctcagcagg gtaaaatcgt cttcaacggg aaagagatca cccgtctgca tacgcacgaa
    12121 cttgtcggcg tgggcatcgg cagaactttt cagaatatcg agctttttcc gggcatgacc
    12181 gttctggcca atatgacact ggcgaggcat attcattgcc ggtacagttt cataaaatca
    12241 tttctctttt ccaaatcagt ccgggatgaa gaaatccgtc accggcaggt ccttgaagag
    12301 atcatcgatt ttctggaaat gcaatccatc cggaagcagg cggtcggttc tctgccttac
    12361 ggcatgcaga agagagttga actgggacgc gcgctggctt tggagccgaa gctgctggtc
    12421 ctggatgagc cgtttgccgg catgaacatg gaagagaagg aggatatggt gcgcttcctt
    12481 ctggagctga atcagcaatg gggcttgacg atgatcctgg ttgaacatga tatgtccatc
    12541 gtcatgagta tttcccagcg gatcatggtt ttgaatttcg gagagaagct gggtgaagga
    12601 aacgctgaag aaatcagcgc aaatcctgaa gttataaagg cttatctcgg cgagtccgaa
    12661 gcaatgtaaa tattttaagg cgttttaatt ttgagcaagg gaacatcaca tatcacgaaa
    12721 cgcaatctga cattgccgca ggtgttaatg gaaaatgccg ccgtgtacgg tgatgcaaaa
    12781 acggctatcc gggaaaaggc atacggtatc tggcagaaat acacctgggc ggattacggt
    12841 cgctatgttc ggatgacggc ggccggattg gcagcactga atctcaggcg cggtgaaaat
    12901 ctgtgcatgg tcgtccataa tcatccggaa tggctctttt cggaactggc cgcgcatgtc
    12961 ctgggggcaa caacgctgaa tttgtttaca tcatccattg ccgacgaact ggcgttttca
    13021 atcaaaagga tcaactcacc cgttgtggtg gttcaggatc aggaacaggt ggataaactt
    13081 ctggagatca aagaaaaact tcctcagacc caaaaggttg tttatatcga tcccacgggc
    13141 atggcttctt atcaggacga tccctggctg atcagttacg ccggactctt aaaactgggc
    13201 gaacaatatc ttgaagaaca tcccggcttt attgactccg aaatcaatcg gggacgcccc
    13261 gatgatattg ccgttatgat ccagacatcg gggaccacag gcgttcccaa gctggccatg
    13321 ctgtcccatc gcaacctcct gaccatggcc aggcaatgga ttgaagaaac caggatcaaa
    13381 ccggaggaaa actggttgtc catgtcgccg ccggcctgga tcgtcgatca gatgtggggc
    13441 atgggtgtgg cgcttgacgg cgccatgtgc atgaacttcc cggaaacggc ggaaaccgtt
    13501 ctggaagatt tcagagaaat cggaccttcg atgatcatta catcgtcgcg gttctgggaa
    13561 gacatggctt cgcgcatccg cgtaaaaatt tccgatgccg gttggctgaa gaagacatta
    13621 ttttatctgg gcgaaaaaat cggcaaatcg gtggtggaaa aacagtcgca ccgacaaccg
    13681 gtcggatggc cgttgcaggt tttgcacagc gtcatgtcca tgattgttta caggccgctt
    13741 ctggatcgtc tgggatgttc gcatttccgc agcgccttta ccggcggaca tccgatcagt
    13801 cccgatgtca ttcatttctt ccgggccatc ggtctgaatt taaaacaatg ctacggtttg
    13861 acggaatcgt gcggcatttt ccagatccag ccggatcatg aagtcaaact ggaaaccgtc
    13921 ggcaaaccca tggtgctgac cgaggtgaaa atcgccgggg atcaggaagt gctggtcaaa
    13981 tccgaagcga atttttcagg ctatcacaat gattatgaat caacgcaaaa cgcgtttgaa
    14041 aacggctggc tcaaaaccgg cgatgccgga tatatcgacc atgacggtca tctgttgatt
    14101 atcggccgca aggaagatat tatccgcaat aaaagcggcg aggctttctc gcctgatttt
    14161 atcgagacgc gtcttaaatt cagtccatat atcaaggaag ccgtgacatt cggcgaagcc
    14221 cgtccttaca ttgcggcgat gatcaacatt gatatgggca atgtcggcaa ctgggcggag
    14281 gaaagaatga ttccttatac gacgtacatg gatctctccc agcagtcggc ggtggaagaa
    14341 ttgattttga aggaagtcat tgcggtgaat gagcagcttc ccgaggtcat gaagattaaa
    14401 aagtttgttc tgctttacaa actgctggat gcggatgatg aggaactgac ccgcaccggc
    14461 aaagttcgcc gccgcttcgt ttacggtttg tatctgaaga tcatagaggc gatgtataac
    14521 aatttgaaaa atgttcaggt tacgggaaaa gtgaagtacc gcgatggaag gatcggcgag
    14581 atagaaacaa cgattaatgt tattaatgta aattaaggga cttattagag atggaatttt
    14641 ttctgcaatt attggtcaac ggcgtttgca tcggactgct ctacggcatt tccgcgatgg
    14701 gctttgtcat gatttttaaa tcgtcaagtg tgttgaattt tgcccacggt gaacttctgg
    14761 cgctgggagc gtttttcttt ctgtcgctgg tcacgtgggc gcagttgccg gtttacgtag
    14821 cttttattct gaccatgatc ggctgtttta ttcttggttt tctcatcgaa agattcttcc
    14881 tgcggccgtt aatcggcgag aaactcattt tcgtgattat gctgactgtg ggactggcgg
    14941 cgatgtttaa aggctttatt ctgctcgtct ggggcggcaa tttgcacacc tatccggaat
    15001 tcctgccgcc atccatggcg atgcagttcg gttccattta tgtcgcgccg gcctattctg
    15061 cgacgctgat gatcagcgtt gtttttctgg tattattcgg actgtttttt aaaaaatcat
    15121 cccagggaat ttatatgcgc tccgtggccg ataatcagaa agccgcgctt tctttgggcg
    15181 ttcatgtccg ccgggtgttt gcgctttcct gggcgattgc cgcgctggtg gcctgtatga
    15241 gcggcattgt tctgggcatc atcaacggcg tcaatgtgca tgatctgagc tccatcggac
    15301 tcaaggtttt cccggttgtt attctgggcg gcctggacag catcggcggc gccattatcg
    15361 gcggaatcat catcggcctt ctggaaacct ttaccggcgg ctacctgtcg ccgtctttgc
    15421 gcgatgttgt tccttatatc gtgctggtgt ttattttaat ggtcaagcct tacggattgt
    15481 tcggcctggt ggaaattgaa agagtgtagg acgtacgaaa aatgaaaaaa ttatcttttc
    15541 atccctgcgg caattaccgg gaaacctacg agcaggaact ga
//
LOCUS       DAOOLB010000044        15490 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4048_length_15490_cov_41.263103, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000044 DAOOLB010000000
VERSION     DAOOLB010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 15490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15490
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4048_length_15490_cov_41.263103"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..364
                     /locus_tag="PLF77_06800"
     CDS             134..364
                     /locus_tag="PLF77_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HPC08597.1"
                     /translation="MEKIIKIHVKKLPEGVYLATSKDVPGLVAQGRTATETLEIARDV
                     AKKLLEARKERRGKIKISEIKDSFDYPMIVSA"
     gene            961..2454
                     /locus_tag="PLF77_06805"
     CDS             961..2454
                     /locus_tag="PLF77_06805"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HPC08598.1"
                     /translation="MNVFVKKGMLAETKSQAVILTLFEDGKKLSGIALEIDKKSGGLI
                     SKLMADGDFEGKASQVAVAYPCGNLPSKRIALVGLGKQKEFNLEKLRAAFAKVMRHLR
                     GMNVKEASTSVNLNLIPGKKDQIVQAAVEGALLGLYQYTPYKTVGREEFKEMDEFNII
                     ADAKEYAVIEPSVKKAKIIADAVCFVRDMISAPSNEMTPSIMAQKAQEVAKRKNVTCT
                     VLDRKKMQELGMNALLAVASGSQEEPKFIILEYSGGRKKDAPVVLVGKGLTFDSGGIS
                     LKPSDKMEEMKTDMSGGAAVMGAIMAAADMQIPLNVVALIPASDNMPSGSAFKPGDIL
                     KSYSGKTIEILSTDAEGRLLLADALAYASKFKPEAVIDLATLTGACVIALGDDVIGML
                     GTGEKLKKEINRAAQDTGELVWELPLWEQYHELIKSDIADYKNAGNRAAGTITAAAFL
                     SKFVGDYPWAHLDIAGPAWADKDKPYIPKGASGIGVRLLVEFLRNRS"
     gene            2464..3453
                     /locus_tag="PLF77_06810"
     CDS             2464..3453
                     /locus_tag="PLF77_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC08599.1"
                     /translation="MPPLLQIKDLRTVFDTVHGRIKAVDGVSLDIASGETLGVVGESG
                     CGKTMLSLSIMRLIPPNGKIINGEILFSGVDLLKLSEEDMRARRGKDIAMIFQEPMTS
                     LNPVFRVGDQIAEAIRLHQQLPAKQAMALSVELLREVGIAEPQKRARDYPHNLSGGMR
                     QRVMIAIAMSCHPQLLLADEPTTALDVTIQAQILDLISNLKQKNNMAVLFITHDLGVV
                     AQAAEKVAVMYAGKIVEYSTVENLFAKPLHPYTQGLLESIPARCSDAKEKQAYLKTIP
                     GSVPDLYRMARSCRFHDRCPFAFAECELQDPPLLAVEDGHLVSCWKYGSEKNN"
     gene            3469..4437
                     /locus_tag="PLF77_06815"
     CDS             3469..4437
                     /locus_tag="PLF77_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC08600.1"
                     /translation="MSSVLVDIQKVDKKYTLGGNVFGAQKRIIHAVKNVDLQIIRGET
                     LGLVGESGCGKSTLARLITRLENPTSGVIAYKGENIFTFTGESLKTYRRSVQIIFQDT
                     YSSLNPRKSALSMIREPLTIHRLGSKKEREETALSIMAKVGLKKEQAHRYPHEFSGGQ
                     RQRIGIARALVLNPELIIADEPVSSLDVSIQAQILNLLRDLKNDFHLTYLFVSHDLNV
                     IRYISDRVAVMYLGKIVELAGGEDIYNRPLHPYTRMLLSAVPSCDPGKRKTSIAIKGE
                     ARETDNEGCAFRNRCPYRIDVCDKTDPALDAISGQSFCACHRAGAI"
     gene            4513..5127
                     /locus_tag="PLF77_06820"
     CDS             4513..5127
                     /locus_tag="PLF77_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08601.1"
                     /translation="MREEKILIKNNALVIEALLHRASGERGAVICHPHSLMGGSMYNN
                     VVEALQEAFASEGISTLRFNFRGVGSSTGSYSEGVGEKEDIWAAGNYLKTSGLREIFF
                     AGYSFGAWVGAGVIAQPDHPFCSSVFVAPPIDYFEFDWPPLKNKIDLMICGDADSFCP
                     WEPLKDASKKINALLEVIGGADHFFMGKEKKLVILLRDYLAKKT"
     gene            5379..5579
                     /locus_tag="PLF77_06825"
     CDS             5379..5579
                     /locus_tag="PLF77_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPC08602.1"
                     /translation="MAEGKVKWFNEKKGFGFIENDEGGDVFVHYSAITGTGFKTLYEG
                     QKIKFDIEQGNKGPSATNVTPL"
     gene            complement(5721..7064)
                     /locus_tag="PLF77_06830"
     CDS             complement(5721..7064)
                     /locus_tag="PLF77_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPC08603.1"
                     /translation="MNKRLDLGKEAILPLILKMSWPSIIAMTAMSLYNFIDTFWLARF
                     SSQALAALTVCFPIQLLAAAIGVGTGIGAGSFSARMFGAEKNLEAQKTAGQTFFLSML
                     FGFGSILFVLLFGDSMLVFFGASDEILPLSHDYLFLAVYSSPFLFFSMMTAIMLRVEG
                     RPLLSMNVVLIALISSAILDPFLIFGIGPFPRMGIRGAALAGIIAHLIMFILSLYFLQ
                     LKSSKYDFRLKYTRPDLQIIKSIYSTGFPSMVTNFVISIVLVIYNHVLGNFGFQAIAA
                     FGICFRVTGLATMVLFGVGAGLMPIVGYNLGARLFQRLRESVSVALKVSVVFSAVSVI
                     ILELFSSQIITFFSSDPHLTAIAIPALRIHVAALIFSAPIIIYVNMFIGLGKGTLAMI
                     ILFIRDILVFVPLLIILPLFWGITGAWMALPLAGFIELFLVIYWGEKELRKLETR"
     gene            7132..8043
                     /gene="miaA"
                     /locus_tag="PLF77_06835"
     CDS             7132..8043
                     /gene="miaA"
                     /locus_tag="PLF77_06835"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPC08604.1"
                     /translation="MAKNLIVILGPTASGKTRLAAQLACDFQGEVISADSRQVYRTMN
                     IGTGKDLKEYVIDGRQIPYHLIDIEDPENEFNVFEFQKRFYAVFSAIRQRKKLPVLTG
                     GTGLYLEAVLCAYDMPEAPINEEIRRELSEKSIEELQDLLCRLKPHLHNRTDLDDKKR
                     LLRAVEIEKARIRNNQDLRDRPEITAAVFGVRWERSVLRNRITKRLEERLENGMIEEV
                     ADLHAAGLSWLRLESFGLEYRFISEYLQQRLTFDEMKNRLNIAIHQFAKRQETWFRRM
                     ERKGVQINWIDNGDYLLLAEKVAKHLK"
     gene            8040..9395
                     /locus_tag="PLF77_06840"
     CDS             8040..9395
                     /locus_tag="PLF77_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08605.1"
                     /translation="MKKRSAFDDYLHTYSVGEKWKLCAVPDQNFSQVVVIPAYAEKDL
                     LFRTLASLAVNVPDALENSFILCVVNHKEDSPASVMENNKITMAYLNALIRRKSLKTF
                     QLTKDVTALLQCISDSKMKLGYIDAASRGYEIPRNTGGVGMARKIGMDMALRLLEKNA
                     PQNGAIVSLDADTLVEPNYLSVVRDYFRKNVKTAIMAYAHQMPENPAEQAAICCYEIF
                     LRYWVLGLRYAKSPWAFHSIGSTMAVAPQSYLAVRGMNKRAAGEDFYFLNKLAKVGRI
                     DYIRETCVHPSARVSTRVPFGTGKRIERFLAGDAAKEYYLYDPQIFSILSGWLSLVHR
                     MIDRDVDDILMEAKRIHPALKAFLEEARFPLRWSAIRRNTKDQETLLNHFHVWFDGFR
                     TLKMINYFSRVAYPPVDMFSALKRIFNLLDLPALSPEVAEDIPSLGEQMEILRFLRTI
                     T"
     gene            9552..9974
                     /locus_tag="PLF77_06845"
                     /pseudo
     CDS             9552..9974
                     /locus_tag="PLF77_06845"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
     gene            10044..10553
                     /locus_tag="PLF77_06850"
     CDS             10044..10553
                     /locus_tag="PLF77_06850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPC08606.1"
                     /translation="MKPTEKDINKFYNDNREKLFVEPESVHVRHILVSVGKDDSEKVK
                     AEKKAKIDDLRKQLVGGANFAEIAGQHSDCPSKQAGGDLNFIRRGQMVKAFEDAAFAQ
                     EKNAIGPVIKTEYGYHIVQVLDRRPAKKIALSEVKSKIADYLERQKKMEAFNNLMAKL
                     KAQAKITVY"
     gene            complement(10578..11396)
                     /gene="panB"
                     /locus_tag="PLF77_06855"
     CDS             complement(10578..11396)
                     /gene="panB"
                     /locus_tag="PLF77_06855"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPC08607.1"
                     /translation="MSTHSKITRKTTADIRKMKTTGEKIAMLTAYDYAMASILDESDI
                     DMLLVGDSLGNVMLGYDSTLPVTMDDMLHHTKAVARAVKKAMVVADMPFLSYQVSPEA
                     ALANAGRFLKEADAQAVKLEGGREFADIVHKMTYAGIPVMAHLGLTPQSVHQLGGYKV
                     QGKKDDAAETIMQDARILEESGAFAVVLECVPEKLAAEITSALSIPTIGIGAGVYCDG
                     QVLVVHDMLGMQEKFTPKFVKKYANLNLEIKKAVKTYIGEVKNSNFPDTEHSFK"
     gene            complement(11383..>12324)
                     /locus_tag="PLF77_06860"
     CDS             complement(11383..>12324)
                     /locus_tag="PLF77_06860"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPC08608.1"
                     /translation="GIWFQLVRCTAMRDHMKKKELNNFAIIGTGMVGTAIGSLLKKSG
                     YNVVSICDKSSTALKRAVSYTGGKPCKTIPDAVGHADCILITTPDDHIASACREIASA
                     SSIAGKYVFHMSGAGGLDLLESSKKAGAFVASIHPLQSFSSIDQAIKNIPGSYFGVTA
                     DRYSLIPAKHIIHKLGGIPMVISNDQKPLYHAAACFASNYLVSLMNIVESIYRSIGIR
                     EKDAQRAYLPLVYGSLKNIEKSGSISSLTGPIARGDYGTIRKHLRTIDKNLPRYSSLY
                     ASLGLITVKIAQQKGTLNAGQARKINALLKGGKHEHS"
     assembly_gap    12325..12424
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12701..13348
                     /locus_tag="PLF77_06865"
     CDS             12701..13348
                     /locus_tag="PLF77_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HPC08609.1"
                     /translation="MMKKIDLMIFDLDGTIASTGDDLVLCINHTLKTLNLRERTKKDI
                     IGFVGDGISKLMERALGSRYREYQEEAMRIFSAYYAEHLLDNTKLYPHVEEVLRNFEN
                     KPKVVLTNKRYPYALMIVRGLKIEKYFTEVIGADSTPFLKPDRRIIDSVLEKHPAERH
                     RALIVGDGVNDIAVARNSGIMSCAFLNGLGRRDDLLKAKADYYCEDIREVNRLFR"
     gene            13351..14766
                     /gene="tilS"
                     /locus_tag="PLF77_06870"
     CDS             13351..14766
                     /gene="tilS"
                     /locus_tag="PLF77_06870"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HPC08610.1"
                     /translation="MIKKVKQTIEKFDLLKKGERVVVAVSGGPDSTACLTVLASIAGD
                     MDLELIVAHFNHGLRGKESDRDEQFARRLAKKLGLVFCAGKLNKKNRAKGISPEDFYR
                     RQRYAFLEKTAAKHRAQKIALGHHLNDQAETVLLNLLRGSGLEGLKGFLPIREGRIIR
                     PLIEVSAQEIISFLEQSGMAYCRDSSNESSQYLRNQIRAELIPFLKGKYNPRIEENLA
                     QMAEILRREDDFIRQHAAAAAKKTMQQKKAGSVALNIHAVKKIPEAIRWRLLKMLLED
                     LTPSGDGISFLHVNAVNDLTQKNASGKKITLPAGIEARREYDQLILEKKRTRPKKNQY
                     AYVMSVPGTVHVKERGLTIKATLVKKIKTDLSRMKNTACLDWSKIAPPLVVRNRRDGD
                     WIQPLGMRGRQKIKKYFIDHKIPVAQRDDLLLLVDRLSVVYIEHRHISDRVKISPATK
                     SVLKLAITPDRPVGKGSKPPG"
     gene            14844..>15490
                     /locus_tag="PLF77_06875"
     CDS             14844..>15490
                     /locus_tag="PLF77_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HPC08611.1"
                     /translation="MNQFQKNIALVLTVLLVFLLVWQLYNQPKTGTKEIIYSEMLTLL
                     DKGEISEVTIQGDHINGKLKNNSLFKSFAPKDDQLVEKFSGKGVKITAKPVEESAWLT
                     ILISWAPMILLIGIWIFFMRQMQAGGGKAMAFGKSKARLITDKSKRVTFADVAGIDEA
                     KSELEEVIDFLKDPKKYTKLGGRIPKGLLLVGPPGTGKTLLARAIAGEAEVPFLT"
BASE COUNT         4268 a         3382 c         4017 g         3723 t
ORIGIN      
        1 aaaatgaaag agatttttaa tataaattca aaggatattg aaaagagagt ataaatccgg
       61 tctgtgcttg acatttactg tattttattt aagaatacag gcatacaaag attaatagaa
      121 tgtaaggaaa atcatggaaa aaatcattaa aattcatgtc aagaaattgc cggaaggggt
      181 ttatttggca acgtccaaag atgttcccgg tttggttgct cagggacgaa ccgccacgga
      241 aacactggag attgcccgtg atgttgccaa aaaattactg gaagccagaa aagaacgccg
      301 tggcaagata aaaatatccg agattaaaga ctcatttgat tatccgatga ttgtgagcgc
      361 gtaacgtggg acgattggcg ggtttccggt atcgtgaaat cgtaaagcgt cttaaatcgt
      421 ttggctttgc ttttgacagg caagcggcgg gaagtcatga aatctggtac aattcattga
      481 acaatcttta tacaaccata cccaatcatc ccggagatat accggaagga acgctgagag
      541 ccatattaaa gcaagctggt attaccccga atgatttctt aaagaacaaa taaggcacat
      601 ttcccctttt gatgcagggc gagatctaac ctaaataccc taaagggcac tatagggtca
      661 tattgtccga tagcgccgac ttgccatatc ggactgatcg ggggatcagt ccctaccttt
      721 attgtgagac tcaaatttca aaaacgtagt gcattgaaat ttgtaagtcg aacaatcccg
      781 cgcagcggag ggtttaatac cccgcagctt gctgcggaat agtttccaaa gcttgctttg
      841 gggttcatac ccgtgattcc taaacatgaa ttctgcttaa tatttgactt tttcagttta
      901 tatgctatcg tccgtcacac attgttacaa cgaagcaatt cttagcagga gggtcttgaa
      961 ttgaacgttt ttgtcaaaaa aggaatgctt gcggaaacca aaagccaggc agtgattctg
     1021 acgttgtttg aagacggcaa gaaattatcg ggcattgcgc tggagattga taaaaaaagc
     1081 ggcggtctta tcagcaaatt aatggccgac ggtgattttg aaggcaaggc gtcgcaggta
     1141 gccgttgctt atccctgcgg gaatcttccg tcgaagagaa tcgctctggt cggtttggga
     1201 aagcaaaagg aattcaatct cgaaaaattg cgcgcggcat ttgccaaagt gatgcgtcat
     1261 cttcgcggga tgaatgtcaa ggaagcgtcg acgtccgtga atttgaatct tattcccggc
     1321 aagaaagatc agatcgtcca ggcagcggtg gagggggctt tgctcgggct gtatcaatat
     1381 accccgtata aaaccgtcgg acgggaagaa ttcaaggaga tggatgaatt caatatcatc
     1441 gccgatgcga aagagtatgc cgtgattgag ccgtctgtga aaaaagccaa aattattgcc
     1501 gacgcggttt gctttgtgcg cgatatgatt tctgcgccct ccaacgaaat gacgccttcg
     1561 atcatggcac aaaaagcaca ggaagtggcc aagagaaaaa atgtgacgtg tacggttctg
     1621 gatagaaaga agatgcagga attgggtatg aatgctctgc tggcggtggc atccggaagc
     1681 caagaagaac cgaagtttat tattctggaa tattccggcg gcagaaaaaa agacgctccc
     1741 gttgtgctgg tgggcaaagg actgacgttc gacagcgggg gaatctccct caaaccgtca
     1801 gataaaatgg aggagatgaa gacggatatg tcgggcggcg ccgcggtgat gggagcgatt
     1861 atggcggctg cggatatgca gattcctctc aatgttgttg cattgattcc tgcgtcggac
     1921 aatatgccga gcggatccgc gttcaagccg ggcgatattt taaaatcgta ttccggcaag
     1981 accatagaaa ttctcagtac ggacgccgag ggacggctgc ttctggccga tgcgctggcc
     2041 tatgcttcta agttcaaacc ggaagccgtc atagacctcg ccacgctgac gggcgcctgt
     2101 gtgatcgcgc tgggcgacga tgtcatcgga atgctgggaa cgggtgaaaa gttgaaaaaa
     2161 gaaatcaatc gcgccgcgca agacaccgga gagcttgttt gggaactgcc gctgtgggag
     2221 caataccatg agctgatcaa gagcgatatc gccgattaca agaatgccgg caaccgtgcc
     2281 gcgggcacca ttacggccgc cgcatttttg agcaaatttg tcggcgatta tccctgggcg
     2341 catctggaca ttgccgggcc ggcctgggcg gacaaggata aaccgtatat tcccaaaggc
     2401 gcgtcaggga tcggtgtccg gttactggta gaatttctgc gcaaccgttc ctgatatcaa
     2461 tctatgccgc cactgctgca aataaaagat ctcagaaccg tgtttgatac cgttcatggc
     2521 cgcataaaag ccgtggacgg ggtatctctg gacatcgcgt ccggggaaac cctgggcgtg
     2581 gtcggcgaat ccggctgcgg caaaacaatg ctttcccttt ccattatgcg tctcattcct
     2641 cccaacggca aaattatcaa cggtgaaatt ctattttcgg gcgtggattt gctgaaatta
     2701 tccgaagaag acatgcgcgc ccgccgcggc aaggatattg ccatgatctt tcaggaaccg
     2761 atgacgtcgc tgaatccggt tttccgcgtc ggcgatcaga tagccgaggc gatccgtctg
     2821 catcagcagc tgccggcgaa acaggccatg gcgttgagcg tggagctttt gcgagaagtg
     2881 ggaattgcag agccgcagaa acgggcgcgg gattatccgc acaatctcag cggcggcatg
     2941 cgtcagcgcg tcatgatcgc catagcgatg tcctgccatc cgcaactgct tctggcggac
     3001 gagccgacca cggcgctgga tgtcaccatt caggcgcaga ttctggatct gatttccaat
     3061 ctgaaacaga aaaataatat ggcggtgctt ttcattacgc acgatctggg cgtcgtcgct
     3121 caggcggccg agaaagtggc tgtcatgtac gcgggaaaaa ttgtcgaata ttccaccgtg
     3181 gaaaatctct ttgcaaagcc cttgcatccc tatacgcagg ggctgctgga atcgatcccg
     3241 gcgagatgtt ccgatgcaaa ggaaaaacag gcgtatctaa aaacgattcc cggctccgtt
     3301 cccgatctct atcgtatggc ccggagttgc cgtttccatg accggtgccc ctttgctttt
     3361 gctgaatgcg aactgcagga tcccccattg ctggccgtgg aagacggtca tctggtttca
     3421 tgctggaaat acggatcgga aaaaaataat taatgaaaag agcaaataat gtccagcgtt
     3481 cttgtcgaca ttcagaaggt agataaaaaa tacacgttag gcggcaatgt gtttggcgct
     3541 caaaagcgaa tcattcacgc cgtcaagaac gtcgatttgc agattattcg gggggaaacg
     3601 ctggggttgg tcggcgaatc gggttgcggt aaatcaacgc tggcccgtct gatcaccagg
     3661 ctggagaatc ccacatcggg cgtcatcgca tataaaggtg aaaatatttt tacgttcacg
     3721 ggcgagtctt taaagaccta tcgccgcagc gtgcagatca tttttcagga tacgtattcc
     3781 tcactgaatc cgcgaaagtc ggctttaagc atgatccggg agcctttgac cattcatcgc
     3841 ctgggaagca aaaaagagcg cgaggaaacg gcgctgtcga ttatggcgaa agtcgggttg
     3901 aaaaaggaac aggcccatcg ctacccgcat gaattcagcg gcgggcagcg gcagcgtatc
     3961 ggcattgccc gtgcgcttgt attgaatccg gaattgatta ttgccgatga accggtttcc
     4021 tcgctggatg tttccattca ggcgcagatt ctgaatctgc ttcgggattt gaaaaacgat
     4081 tttcatttga cgtacctttt tgtttcccat gatctcaacg tcatccggta tatttccgac
     4141 cgggtggccg tgatgtacct ggggaagatt gtcgaactcg cgggcggcga agatatttac
     4201 aaccgtccgc tgcatcctta tacacgcatg ctgctttcgg ccgtgccgtc ctgcgacccg
     4261 ggtaaaagaa aaacgtccat tgccataaag ggcgaagcga gggagacgga taacgaaggg
     4321 tgcgctttcc ggaaccgctg tccctacaga atagacgtct gcgataaaac ggatcctgct
     4381 ctggacgcca taagcggcca aagtttttgt gcctgccaca gggccggtgc aatttaaaat
     4441 agcgtttgtt tcctttctgt catgtcgcgt atcatgacgg aaagttaaat ttcgaaaagg
     4501 tctccgtgtg ttatgcggga agaaaagatc ctgataaaga ataatgctct ggtgattgaa
     4561 gccttgctgc atcgggcatc gggcgaacga ggtgcggtga tctgccaccc gcattcgctg
     4621 atgggcggtt cgatgtataa taatgtcgtt gaagcgctgc aggaggcgtt tgcatcggaa
     4681 gggatttcca ccttgagatt caactttcgg ggcgtgggct ccagcaccgg gtcgtatagc
     4741 gaaggcgtgg gagagaagga agatatttgg gctgccggca actatctgaa aacgtcaggt
     4801 ctgcgcgaga tattttttgc ggggtattcg ttcggagcct gggtcggtgc cggggtgatt
     4861 gcgcaaccgg atcatccttt ttgcagcagc gtctttgtag cgcctccgat agattatttt
     4921 gaatttgact ggccgccttt aaaaaataaa attgatttaa tgatctgcgg cgatgccgat
     4981 tctttctgtc cgtgggagcc tttgaaggat gcgtcaaaaa aaataaatgc gttgctggaa
     5041 gtcattggcg gcgcagatca tttttttatg gggaaagaaa aaaaacttgt tattcttttg
     5101 cgggattatc tggctaaaaa aacataaatt aaataaaaaa acttgatttc aaaaaaaaaa
     5161 gatgataata tttcatacaa cgcataaaaa accgaatata agaaacacgt caaagatagg
     5221 atgagaacgg ttttttttag gaagttgtta gtgttaagtt gtataataaa taagacaacc
     5281 gcggaggatt cttaaaaaat atagaattct tgcggttttt tttgtttcct gtacaaacat
     5341 taaaacagga aatcattttt taaaggaggg ttttagcttt ggcagaagga aaagtaaagt
     5401 ggttcaacga gaagaagggg tttggtttta ttgaaaatga tgaaggtggt gacgtgtttg
     5461 tacattatag tgcgattacc ggaacgggat ttaagaccct ttatgaaggt cagaaaatca
     5521 agttcgatat tgaacagggg aataaaggtc ccagcgcaac aaatgttacg ccactgtaat
     5581 actcgcaatt gatttattct aactgaaaaa atgccccgtg atgaacgggg tatttttttt
     5641 caacgattcc ggatcttaag cagccgcatt ggatcacagg accgggcgtg aagtatgccg
     5701 tcttgtatat caaaaaatat ttaacgggtt tcaagtttcc gaagctcttt ttctccccag
     5761 taaataacaa gaaataattc aatgaagccg gccaggggca atgccatcca ggctccggtg
     5821 attccccaga ataaaggcag gataatcagt aacggaacaa agaccagaat atcccggatg
     5881 aaaagaataa tcatggccag cgtccctttg ccaagtccaa tgaacatgtt gacgtaaatg
     5941 atgatgggag cggaaaagat cagggctgcg acatggatgc gtaacgccgg tatggcaatg
     6001 gcggttaaat ggggatcgct ggaaaaaaaa gtgatgatct gggaagaaaa caattccagg
     6061 atgatcacgg aaaccgcgga aaaaacaaca gagactttca aggcgacgga gaccgattcc
     6121 cgtaatcgtt gaaagagcct cgcgcccagg ttatatccga caatcggcat taatcccgcg
     6181 ccgacgccga acagtaccat ggtggccagg ccggtaaccc ggaaacaaat gccgaaggcc
     6241 gcgatggcct gaaaaccgaa attgccgaga acatgattgt agatcaccag aacaatgctg
     6301 atgacaaaat tggtgaccat cgaggggaaa ccggttgaat aaatggactt gatgatctgc
     6361 agatcgggac gggtgtattt cagtctgaaa tcatacttgg atgatttcaa ttgcaaaaaa
     6421 taaagggaca agataaacat gatcaagtgc gcgatgatac cggccagcgc agcgcctcgg
     6481 attcccatgc gtggaaaggg gccgatgccg aaaatcagaa aaggatcgag gatcgcgctg
     6541 gaaatcaggg caatcaggac aacgttcatg gataaaagag gacggccttc gacacgcagc
     6601 atgatggccg tcatcatgga aaaaaagaga aaaggcgagc tgtaaacggc cagaaaaaga
     6661 taatcatgac tcagaggtaa tatttcgtcg gacgcgccga aaaagacgag catggaatcc
     6721 ccgaaaagaa gcacgaaaag aatgcttccg aagccgaaca gcatggaaag aaaaaatgtc
     6781 tgcccggcgg ttttctgtgc ttccagattt ttttcggcgc caaacatcct tgcggaaaat
     6841 gaaccggctc cgatgccggt tccgacgcct atcgcggcgg caagcagttg aatgggaaag
     6901 cacacggtca gtgcggcaag tgcctgagag ctgaaacggg caagccagaa ggtgtcgatg
     6961 aaattgtaaa gagacatggc tgtcatcgca atgatcgacg gccagctcat ttttaaaatc
     7021 agcgggagga ttgcctcttt tcctaaatcc agtctcttat tcattatggg tcatttcatt
     7081 tgaatgcgtt tagttgctag catataaaga attagaatat caagataaaa tatggcaaaa
     7141 aatcttattg ttatcctcgg tccaacggca tccggcaaaa cccgcctggc ggcacaactg
     7201 gcctgtgatt ttcagggaga agtgatttcc gcggattccc gccaggtgta cagaaccatg
     7261 aacatcggca ccggaaaaga tttaaaggaa tatgtgattg acggccggca gattccttat
     7321 catctgattg atattgaaga cccggaaaac gaatttaatg tattcgagtt tcagaaacga
     7381 ttttatgcgg tgttcagcgc tatccgccaa agaaagaaac tgccggtcct tacgggcgga
     7441 acagggctgt atctggaagc ggttctttgc gcatatgata tgcccgaagc gccgatcaat
     7501 gaagaaatca ggcgtgaatt atcagagaaa tcgatcgaag agcttcagga tcttctgtgc
     7561 cgtttgaagc cgcatctcca taacagaacg gatcttgatg ataaaaaacg actgctccgg
     7621 gctgtcgaaa ttgaaaaagc gcggattcgc aataatcagg atttgaggga caggcctgaa
     7681 attacagcgg ctgtcttcgg ggtgcgatgg gaacggtcgg tcctcagaaa ccgcatcaca
     7741 aaacggcttg aagagcgact ggaaaacggg atgattgaag aagttgccga tttgcatgcg
     7801 gccggtttga gctggcttag gctggaaagt ttcggcctgg aataccgttt tatttcggaa
     7861 tatctgcagc agcggcttac gtttgatgaa atgaaaaatc ggttaaacat cgcgattcat
     7921 cagtttgcca aacgacagga gacatggttc cggcgcatgg agagaaaagg tgttcaaatt
     7981 aactggatcg ataacggcga ctacttgctt ctggcagaaa aggttgccaa gcatttaaaa
     8041 tgaagaagcg ttccgcattc gatgattacc tgcacacgta ctccgtcggc gaaaaatgga
     8101 agttatgtgc cgtcccggat caaaatttct ctcaggttgt cgtcattccg gcgtacgccg
     8161 agaaagacct gcttttccgc acgctggctt ctttagccgt caatgtgcct gatgcgctgg
     8221 agaattcttt tatcctgtgc gttgtcaatc acaaagaaga ttcccccgct tccgtcatgg
     8281 aaaacaataa aataaccatg gcgtatttga acgctctgat cagacggaag tctctgaaaa
     8341 cgtttcagct taccaaagac gtcacggctc ttttgcagtg catatcggat tcaaaaatga
     8401 agctgggata tattgacgct gcctcgcggg gatatgaaat accgcgcaat acaggcgggg
     8461 tcggcatggc caggaaaata ggcatggaca tggctttgcg ccttttggag aaaaatgcgc
     8521 cgcaaaacgg cgcgatcgtc agcctggatg ccgataccct ggttgaaccg aattatcttt
     8581 ccgtcgtccg ggattatttc agaaagaatg ttaaaaccgc gattatggcc tatgcacacc
     8641 agatgccgga aaatcccgcg gaacaggcgg ccatttgctg ctatgaaatt ttcttgcgtt
     8701 actgggttct ggggctgcgg tatgcaaaat ctccctgggc tttccattcc atcgggtcga
     8761 ccatggcggt tgccccgcaa tcgtatctgg ccgtaagggg gatgaacaaa agggcggcgg
     8821 gagaggattt ctactttctc aacaagctgg caaaagtcgg ccgcatcgac tacatcaggg
     8881 aaacgtgtgt tcatccgtcg gcgcgcgtat caacgcgggt tcccttcgga acaggcaaac
     8941 gcatcgagcg ctttcttgcg ggagacgccg caaaagaata ttacctgtac gatccgcaga
     9001 tattttcaat attgtccggc tggctcagtc ttgtacatcg gatgattgac cgggacgtgg
     9061 atgacatctt gatggaggct aaacggattc atcccgcctt aaaggccttt ctggaagagg
     9121 cccggtttcc tctgcgctgg tccgccatac ggcgcaacac aaaagatcaa gagactcttt
     9181 tgaaccattt tcatgtctgg ttcgacgggt tcagaacgtt aaaaatgatc aattattttt
     9241 cccgggtggc ttatcctccg gtggatatgt tttcagcatt gaagcggatt ttcaacctgt
     9301 tagatctgcc tgctttgagt ccggaagtcg cagaagatat tccttctttg ggcgaacaaa
     9361 tggaaattct gcgctttctg agaacaatca cctgaacgtc atttagtacc ggaaaattct
     9421 cttgacggat acggataatt tacatataac acgtgaaaat ctttaaaatt tgcaaaattt
     9481 tcgggaggaa attcatgaaa acttggatcg gctcaaaatg gcccataatt tttgttttgt
     9541 ttgtttttgt catggctgcc ggttgttcgg atgccaaaaa aggtgcggag aacgcagttg
     9601 gtgcaaacag tgttgaaagt ttggatgcag gacagccctc gacggttact gatgcggctg
     9661 cccaaaacat tccggcaggc gatctcgccg tcacggttga cggaaaacat atgaccaagg
     9721 ccgaactgga taaggcggtc aaagaaaaat tcgagcttat caaaaaacag gtgccggccg
     9781 acaaacaaaa agcattcagg gaaaatctca gaaagcagtt ggtggacgtc tttgtcatga
     9841 agacacttct ggccaacgaa gtggagaaaa gaaaaatcgt agctactgat caggatgtca
     9901 aagcggccat gaatcaaatc aaagccagcc ttccgccgaa taaaaatgtc gacgactttc
     9961 ttaaggaaaa taaaatttcc aaagaagaca ttattctggc cgtcaagatc gataaattca
    10021 gaaatgcgga agggggcaag gcgctgaaac ccacggaaaa agacatcaac aagttttata
    10081 atgacaatcg tgaaaaacta tttgtcgaac cggaaagcgt gcatgttcgc cacattctgg
    10141 tatctgtcgg caaggacgac agcgagaaag tcaaggcgga gaaaaaagca aaaattgacg
    10201 atctgcgcaa acaattggtc ggcggtgcaa attttgcgga aatcgccggt cagcattccg
    10261 attgccccag caagcaagcc gggggcgatt tgaattttat caggagaggc caaatggtga
    10321 aagcgttcga ggatgccgct tttgcgcagg agaaaaatgc catcggtccg gtcatcaaga
    10381 cggaatacgg ttaccacatc gttcaggtgc tggaccgcag accggcgaag aaaattgcct
    10441 taagcgaagt caaaagcaaa attgccgatt atctggaaag acagaaaaaa atggaagcct
    10501 tcaacaacct gatggccaag ctgaaggcgc aggccaaaat aaccgtttat taaacatcat
    10561 tacaacgttt tacagagtta cttgaaactg tgttccgtat cggggaagtt gctgttcttg
    10621 acttccccga tatatgtttt gaccgctttc ttgatttcca gattgaggtt ggcgtatttt
    10681 ttgacaaatt tcggcgtgaa tttttcctgc atgcccagca tatcgtgaac gaccagcacc
    10741 tgtccgtcgc aataaacacc ggcgccgatg ccgattgtcg gaatagacag ggcgctggtg
    10801 atttcagcag ccagtttttc cggcacgcat tccagcacaa cggcaaacgc gcccgattct
    10861 tccagaatcc tggcatcctg catgattgtt tcagcggcgt cgtctttttt gccctggact
    10921 ttatatcctc ccaactggtg aaccgattgc ggtgtcagac ccagatgcgc catgacggga
    10981 attcccgcat acgtcatttt atgcacgatg tccgcaaatt cccggccgcc ttccagtttc
    11041 acagcctggg catcggcttc cttcaggaac cgtcccgcat tggcgagcgc cgcctcagga
    11101 gatacctgat acgataaaaa aggcatatcc gcgacgacca tcgccttctt aaccgcacgc
    11161 gcaacagctt tggtatggtg aagcatgtcg tccatcgtga ccggcaaggt tgaatcgtag
    11221 ccgagcatga cgttccccag agaatcgccg accagaagca tgtcaatatc gctttcatcc
    11281 agaatagagg ccatggcgta atcgtaagcc gtcagcatcg caattttttc tcccgttgtt
    11341 ttcatctttc tgatatcagc ggttgtttta cgcgttattt tactatgagt gctcatgctt
    11401 tcctcctttt aaaagggcgt tgatttttct tgcctgtccg gcgttgaggg ttcctttctg
    11461 ttgcgcaatc ttgacggtta tcaatcccag agaagcatat aacgaagaat accggggaag
    11521 atttttatca atggtccgca gatgtttccg gatggtgccg taatctccgc gtgcgatggg
    11581 accggtcagg gaggatatgg aaccggattt ctcgatgttt ttcaaactgc cgtataccag
    11641 gggcaagtaa gctctttgtg cgtctttttc cctgatgcct atggatcggt aaatcgattc
    11701 cacaatgttc atcagcgata caagataatt ggaagcgaaa caggcggccg cgtgatagag
    11761 cggtttctga tcgtttgaaa tgaccatggg gatgccgccc agtttatgaa tgatatgttt
    11821 ggccggaatt aaggaatatc tgtcggcggt aacgccgaag tagcttccgg gaatgttttt
    11881 gatggcctgg tcgatggatg aaaagctttg taacggatga atgcttgcca caaaagcacc
    11941 ggcttttttt gacgattcca gaaggtcaag gccgccggcg ccgctcatgt gaaacacata
    12001 ttttcccgcg atggatgacg cggaggcgat ttcccggcag gccgaggcaa tatgatcatc
    12061 cggggttgta atcagaatac aatcggcgtg tccaacggca tccggaatcg ttttgcacgg
    12121 ttttcctccg gtataggaaa cggctctttt aagggcggtc gaagatttgt cgcagatcga
    12181 aacgacgttg taaccggatt ttttcaacag agagcctatt gcggtgccga ccatacccgt
    12241 gccgatgatg gcaaagttat ttaactcttt tttcttcatg tgatcacgca tcgctgtgca
    12301 tctgactaac tgaaaccata tcccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12421 nnnngtctca aatcccacgt cgctacgctc ctgggataat tcgctctaag attttccgtg
    12481 cactacgttt cggaaaatca aggctcatta aaaaaggcct tccgcaaggg aagaccttta
    12541 tccgacattt cagttcaata ccgtctcagt cgataagaga gatccaagcg gttagaactt
    12601 atctaacacc gacccaaagc ttagtcaaac actttttcat aaagaatctt gtgtttacgg
    12661 acgcacttgg ttatataact taacaaatgc gaagaggttc atgatgaaga aaatagatct
    12721 gatgatattc gatttggacg gtaccattgc ctcgacgggc gatgacctgg ttttgtgcat
    12781 caaccatacc ttaaaaaccc tgaacctcag ggaaagaacg aaaaaggaca ttatcggctt
    12841 tgtcggcgac ggaatcagca aattaatgga gcgggctttg ggaagccgct atcgcgaata
    12901 ccaggaagag gccatgcgga tattttccgc gtattacgcg gagcatcttc tggataatac
    12961 gaaactgtat ccccatgtgg aagaggtttt acggaatttt gagaacaaac cgaaagtcgt
    13021 tctgaccaac aaacggtatc cttatgcgtt gatgattgtc agagggctga agatagaaaa
    13081 atatttcacg gaagtcatcg gcgccgattc aactcctttt ttaaaaccgg atcgtcgtat
    13141 tatcgatagc gttctggaaa aacatccggc cgaacggcac agagccctca tcgttggcga
    13201 cggcgtcaac gatattgcgg tcgccagaaa ttccggaata atgagttgcg cttttttaaa
    13261 cggattgggc cgtcgcgacg atttgctcaa agcgaaggcc gattattact gtgaagacat
    13321 tcgggaagtg aatcggcttt ttagatagtt atgatcaaaa aagtgaaaca gaccatcgag
    13381 aagtttgatc tgctgaaaaa aggagagcgg gttgtcgttg ccgtttccgg cggacctgat
    13441 tcgaccgctt gcctgaccgt tctggcttcg atcgccgggg acatggacct ggaactcatc
    13501 gttgcgcatt tcaatcacgg cttgagagga aaggaatccg accgggatga acagtttgcc
    13561 cgccgcctgg ctaaaaagct ggggcttgtt ttctgtgcgg gaaaactgaa taaaaaaaat
    13621 cgtgccaaag gcatttcgcc ggaagatttt taccggcgtc agcgatatgc ctttctagag
    13681 aaaacagcgg caaagcaccg ggcgcagaaa attgcgctgg ggcatcattt aaatgatcag
    13741 gcggaaacgg ttttgctgaa tttattgcgg ggcagcggtt tggaagggct caaagggttt
    13801 ctgcccatca gggaggggcg gatcattcgt ccgctgattg aagtatccgc acaggaaatc
    13861 atttcttttc tggagcagtc gggcatggcc tattgccggg atagttccaa tgaaagcagt
    13921 caatatctac gcaatcagat ccgggcagag ctgattcctt ttttaaaagg gaaatacaat
    13981 ccgcgaatcg aagagaatct ggcgcaaatg gctgaaattc ttcgccggga agatgatttt
    14041 atccgtcagc atgcggccgc agccgcaaaa aaaaccatgc agcagaaaaa ggccggttct
    14101 gtggcgttga acatccatgc ggttaaaaaa attcccgaag ccatccggtg gcgattgctg
    14161 aagatgcttc tggaggatct gacgccttca ggcgacggca tatcgtttct tcatgtgaat
    14221 gcggtgaatg atctgacgca gaaaaacgca tccggaaaaa agatcacgct tccggccgga
    14281 atcgaagcga gacgcgaata cgatcagtta atcctggaga agaaaaggac gcgtccaaaa
    14341 aaaaatcaat acgcctatgt gatgagcgtt ccgggaacgg tccatgtgaa agagaggggg
    14401 ctgacgatta aggcgacact ggttaaaaaa attaagacgg acttatcccg catgaaaaat
    14461 acggcgtgtc tggattggag taaaatcgct cccccgctgg tggtcagaaa cagacgcgac
    14521 ggagactgga tccagccttt ggggatgcgc ggccggcaga aaattaaaaa atatttcatc
    14581 gatcataaga ttcccgttgc tcaaagagac gacctcctgc tgctggttga ccgtctttcc
    14641 gtcgtctata ttgagcatag gcatatcagt gaccgtgtca agatttcacc ggcaacaaaa
    14701 tcggtcctga aactggcgat cacgcctgac cggcctgtcg ggaaaggttc aaagccgccc
    14761 ggataaaatc catagaaatt atttgagata aattcaatat tattataaga gtaaaataac
    14821 tgttctaaag gagattaaat atattgaatc agtttcagaa aaatatagcg cttgtcctga
    14881 ccgtcctgct ggttttcctg cttgtctggc agctttacaa tcagcctaaa acgggaacca
    14941 aggaaatcat atacagtgag atgttaaccc tgttggacaa gggagagatc agcgaagtca
    15001 ccattcaggg cgatcatatc aacggaaaat tgaaaaacaa cagtcttttt aagtcgtttg
    15061 cgcccaaaga cgaccaactg gttgaaaagt tcagcgggaa aggcgtcaag ataactgcca
    15121 aaccggtgga ggaatccgcc tggctgacca ttttgatttc atgggcgccg atgattctgc
    15181 tgatcggtat ctggatcttt tttatgcgtc agatgcaggc cgggggcgga aaggccatgg
    15241 ctttcggtaa aagcaaggcg cgtctgatca ccgataaatc caagcgggtc acgtttgccg
    15301 atgtggccgg aattgatgag gcgaaatccg aattggaaga agtgattgat ttcctgaagg
    15361 atcccaaaaa atacaccaag ctcggcggca ggattcccaa gggattgttg ctggtcggcc
    15421 ctcccggaac gggaaaaacg cttctggccc gggccattgc cggagaggcg gaagtgccgt
    15481 ttttgaccat
//
LOCUS       DAOOLB010000045        14728 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4353_length_14728_cov_49.084168, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000045 DAOOLB010000000
VERSION     DAOOLB010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14728)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14728)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14728
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4353_length_14728_cov_49.084168"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="PLF77_06880"
     CDS             <1..720
                     /locus_tag="PLF77_06880"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08612.1"
                     /translation="ADSAALLLAQDKQRVKALLDQCAIPTPAWKIFDAPDPQGWKIFP
                     AIVKPQNEHCSAGITPESVVLQPTDLQKRITFVLDHYAQPALVEDFIDGREFHVAVWG
                     NETVNVLPAAEMDFSRFGDLNDRLCTYEAKFTPGSLHYEEIKTLLPAPLTTGEKKSLE
                     TVCRNAYRASGCRDYARMDIRLRDNIFYVLDVNPNADISSDASMACAAEADGISYAEM
                     GSRIVEMAAKRHPVLGKLFRS"
     gene            777..1793
                     /locus_tag="PLF77_06885"
     CDS             777..1793
                     /locus_tag="PLF77_06885"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF002699.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HPC08613.1"
                     /translation="MNKKDFLKTKIEHIDIKKINAVDIVSAMKKMSFSARDLANAADI
                     YDRMLKEKNCAVILTLAGSTSAGGCMQVYVDLVKNNMVDAIVATGASIVDMDFFEALG
                     FRHYQGTPFVDDKLLRKLYIDRIYDTFISENDLQACDKTIKTIADGLAPRPYSSREFI
                     AEMGRYLTKNAKKNNSLVQAAYENNVPVFCPAFSDSSAGFGLVLHQHEHPGNHMSIDS
                     VRDFRELTDIKIKAGTTGLLMIGGGVPKNFAQDTVVCAEILGYDVPMHKYAIQITVAD
                     VRDGACSSSTLKEANSWGKVDSSCEQMVYAEATMALPLLASYAYHKGNWKKRKKWNLS
                     RIFK"
     gene            2128..4617
                     /locus_tag="PLF77_06890"
     CDS             2128..4617
                     /locus_tag="PLF77_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC08614.1"
                     /translation="MEDKNERTKLTERLFSSEDNNLPLLAKDIISNAGVAIYIVHNSQ
                     FVYVSELYQKLTGYAEKELIGEYSLKNIHPDDRDKVRKQAIKCLKKESFEPYEYRYIR
                     KDNEIRYILETVAPIIHKGERATLGSFMDITERKIIEEKYRNILENIQEGYFEVDLEG
                     NFTFFNNAVSRVLGYSREELMGMNNRQYTPDKDSARKVFQAYHHVYETGEPLNAVGWH
                     IITKQGAKRYIEGSISLLKDSQGKPIGFRGITHDVTERKVAEEALRQSEEKYRNILEN
                     IQEAYFEVDLAGNFIFFNDSLCRITGGTREELMGANYTRFSDPATSKNVFLAFNKIFK
                     TGEATEGFDWLIIRKDGSKKYIEASASLNKDASGKPIGFRGVIRDITERKRIEQELNH
                     MATHDALTGLPNRLMFSQLLDHAIQSAQRNKKHLAVFFIDLDRFKIINDSLGHEAGDT
                     LLKEMALRFKQSLRTVDIIGRLGGDEFTILVEDFNDLKQVEFLAKKILNTVMQPIALM
                     GEECRVTASIGISVFPKDGLDEQSLMKNADIAMYFAKSEGKNNFQFYSENIRSMSNER
                     LTIETNLRHAMERNEFYLDYQAKLDLQTRAITGVEALLRWNNPYLGLVAPTQFIPIAE
                     ETGMIIPIGRWVIKTACMQNVAWQKQGLPPVCMSVNLSLRQLMYENFAEDVKTILNET
                     RMASELLELEITESTVMHNPKRLVPILSKLKKMGIRMAIDDFGTGYSSLAQIKNFPIN
                     TLKVDRSFIRNLPQDTDNKAITEAVIFMGKSLKLKVVAEGVETREQAKFLKDQICDEM
                     QGFYFSKPIGPDGFADLLRENSVSGTDPGRQ"
     gene            complement(4663..5187)
                     /locus_tag="PLF77_06895"
     CDS             complement(4663..5187)
                     /locus_tag="PLF77_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HPC08615.1"
                     /translation="MALGIFEDEKPPRGIGGLIDWRLNGMLSREIKNGRIGGGFKEKV
                     VIPFPERIGSEILLLFGLGRVPDITYDRIYDVAYTVTQIIDSMAIKSFAFDLYGEGRA
                     NLVTSDIVEAAVTGIFDFLASDIEKLSEMNACAVTSPSNLHSVAQGVRQFKNNVNDKG
                     SVDISPLEECLAKN"
     gene            complement(5192..5647)
                     /gene="nusB"
                     /locus_tag="PLF77_06900"
     CDS             complement(5192..5647)
                     /gene="nusB"
                     /locus_tag="PLF77_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPC08616.1"
                     /translation="MCGRRKAREIALQVLYSLNFVDIPAQEALDLFWGNFVAPKSAKD
                     FAAFLVMGTCDHRKELDQLIAGCSDNWSLGRMSRVDINILRLAVFELLYCDDIPPKVT
                     MNEAIDLGKIFGSENSGSFINGILDALNLKLNKKNAAKSLDRKPRSSKT"
     gene            complement(5724..6188)
                     /gene="ribE"
                     /locus_tag="PLF77_06905"
     CDS             complement(5724..6188)
                     /gene="ribE"
                     /locus_tag="PLF77_06905"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HPC08617.1"
                     /translation="MLKIIEGKTVAKGMKFGIVASRFNDFICGRLIEGTIDALIRAGA
                     EEKDIVIYKVPGSFELPLTAKKLAKSGRFDAVICLGAIIRGATPHFEYISAEVSKGIA
                     VVGLETEVPVVFGVLTTDTIEQAIERAGTKSGNKGADAAMTAIEMVDLFKKI"
     gene            complement(6236..7438)
                     /locus_tag="PLF77_06910"
     CDS             complement(6236..7438)
                     /locus_tag="PLF77_06910"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HPC08618.1"
                     /translation="MAISTIQEAIEDIKNGKMIILVDDEDRENEGDLCMAAQFATAKT
                     INFMARYGRGLICLTLNEDMADKLHLKQMVQDNQCRFGTAFTISIEARHGVTTGISAA
                     DRATTIQAAVNPEAKPDDLVSPGHVFPIRAKKGGVLVRTGQTEGSVDLCRLAGLTPAG
                     VICEVMKDDGTMARMPDLEIFAKEHKLKIVTIADLIDYRMQNESLIKRMAEATLPTSF
                     GGDFKMIVYENEVDDWQHIALVKGDIKEDDEVLVRVHSECLTGDLFGSLRCDCGDQLH
                     RAMNMVEKEGKGIIVYMRQEGRGIGLVNKIKAYALQEQGKDTVEANIELGFKPDLRDY
                     GIGAQILADLGVRKMRLLTNNPKKIVGLEGYGIEVVKRVPIEIDPNENNIHYMKTKKK
                     KMGHLLKI"
     gene            complement(7457..8086)
                     /locus_tag="PLF77_06915"
     CDS             complement(7457..8086)
                     /locus_tag="PLF77_06915"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HPC08619.1"
                     /translation="MFTGIVEGLGKVRRLTMKGADAVLDIDAGISLEDVSLGDSIAVN
                     GACLTVTSLQQNNFQADVSAETLSRTTLKLLQAGHKVNLEKSLRVGGFVGGHFVLGHV
                     DATARILSRMQKSGSLIIAVEMNDTISRYIVEKGSVAIDGISLTVNKLEKSRFYVNII
                     PHTAVNTTLVTRKEGEWVNIETDILGRYVENLLQSNKGIDKDFLVKHGF"
     gene            complement(8089..8550)
                     /gene="nrdR"
                     /locus_tag="PLF77_06920"
     CDS             complement(8089..8550)
                     /gene="nrdR"
                     /locus_tag="PLF77_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003762439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HPC08620.1"
                     /translation="MKCPFCGHTENKVIDSRISKDGKAVRRRRECLGCTKRFTTYEYV
                     EDVLPMVVKKDGRREQFDRQKILTGIKKACEKRPISMEAIDKLVENIEQACQEMQAEE
                     ISSTVIGEKIMSELKNFDGVAYVRFASVYRQFRDVGEFMSELKDLLSKGKK"
     gene            complement(8571..9056)
                     /locus_tag="PLF77_06925"
     CDS             complement(8571..9056)
                     /locus_tag="PLF77_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HPC08621.1"
                     /translation="MMEIKSSAAVLREKKKKRPDWDSYFLDIVDLVSRRSTCCRRSVG
                     AGLVRDSRILATGYNGAPSKLQHCLDIGCLREQLKVPSGERHELCRGLHAEQNAIIQA
                     ALHGVSTKGSTLYCTNHPCVICAKMIINAGIIRVVIRDGYHDQLAAEMLKEAGIRVEQ
                     L"
     gene            complement(9090..10340)
                     /gene="glyA"
                     /locus_tag="PLF77_06930"
     CDS             complement(9090..10340)
                     /gene="glyA"
                     /locus_tag="PLF77_06930"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPC08622.1"
                     /translation="MSFLEKVDPEVMKAIDLETRRQAGKIELIASENFVSEAVLEAQG
                     SVMTNKYAEGYPEKRYYGGCEYVDIAESLAIERCKKLFGADHVNVQPHSGTQANMAVY
                     FAVAQPGDTILGMNLSHGGHLSHGSPANFSGKFYKIVPYGVNRETETIDFDEVEKLAK
                     EHKPKMIIVGASAYPRTIDFDKFRKIADEVGAVIMADIAHIAGLVCTGLHPSPVPVCE
                     FVTTTTHKTLRGPRGGVVMCKEAYAKTLNSRVFPGMQGGPLMHVIAAKAVAFKEALQP
                     AFKEYQAQIIKNAQAMADELRNQGFRLVSGGTDNHLMLVDLTDKNVTGKDVQDALDRA
                     SITVNKNGIPFDTKGPQVTSGIRIGTPAVTTRGMKEDEMRLIASCIADVIKNISSEQK
                     ITSVAQKIKVLCDRFPLYQGRLKN"
     gene            complement(10467..11726)
                     /gene="fabF"
                     /locus_tag="PLF77_06935"
     CDS             complement(10467..11726)
                     /gene="fabF"
                     /locus_tag="PLF77_06935"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPC08623.1"
                     /translation="MKRRVVVTGLGALTPLGNSLHESWGAAIAGKSGIGLITKFDSTA
                     FKTKIAGEIKNFDPLQYVSKQEARRYDNFLLYSIAASEMALADARLTIGSDISERTGV
                     IIGSAIGGLATLEEEFKNLLDSGPRKISPFAVPAILANLASGQVSIRFGAKGPINCAV
                     TACASGTSAIGDAYKTIAYSDADVMIAGGVEAAVTQLAVGGFGSMRALSVRNDDPQKA
                     SRPFDKDRDGFIIGEGSGIVILEELSFALNRGARIYAELAGYGCTSDAFHMAAPPPGH
                     EGAARCMRVAIQDAGLNPEDIDYINAHGTSTPLNDLYETQAIKSLFGDHAKTLLVSST
                     KSMTGHMLGAAGGVEAIFAVKSLQESVIPPTINLDNPGEGCDLNYVPNTACRQAIHTA
                     MSNTFGFGGVNAVLLFKKFNESQFHKA"
     gene            complement(11750..11986)
                     /gene="acpP"
                     /locus_tag="PLF77_06940"
     CDS             complement(11750..11986)
                     /gene="acpP"
                     /locus_tag="PLF77_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPC08624.1"
                     /translation="MTLEEKVINIIVEQLDVTKEECVLEASFIDDLGADSLDIVELLM
                     EMEEAFGIEIADEELEKIATIQDVVDFLRQKGVQ"
     gene            complement(12153..12338)
                     /gene="rpmF"
                     /locus_tag="PLF77_06945"
     CDS             complement(12153..12338)
                     /gene="rpmF"
                     /locus_tag="PLF77_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPC08625.1"
                     /translation="MPNPVKRHSKTRRNQRRANDFLKSPSMSVCPQCNEPKMPHRICP
                     TCETYKGREYRKAEKND"
     gene            complement(12358..12900)
                     /locus_tag="PLF77_06950"
     CDS             complement(12358..12900)
                     /locus_tag="PLF77_06950"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPC08626.1"
                     /translation="MSNSLKINVLLIPEEGQRFVFSEGDAWFKTCFHKEERLDFALET
                     IDVDCMITKTSGTVFIKGNFSALIDADCSRCLERTRLTIGSNFTYTLIPVKAEQKEEI
                     ELKPEELEISSYQGDFIDLAPIICEQIILQIPIKILCREECKGLCQRCGTNLNAASCQ
                     CEKDVRDTRMAVLKNFKIKN"
     gene            13132..14532
                     /gene="gltX"
                     /locus_tag="PLF77_06955"
     CDS             13132..14532
                     /gene="gltX"
                     /locus_tag="PLF77_06955"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPC08627.1"
                     /translation="MQKIRTRFAPSPTGFLHIGGARTALFNWLYARHLGGQFILRIED
                     TDRQRSTEESTKAILDAMSWLGLNWDEGPHFQAQRVQLHRDMVKKLIDEGKAYYCTCS
                     PEELEIKRKQALANGKKPKYDGTCRNRSLAKSANSVVRFRSRDDGATVVEDMIKGNII
                     FNNEELDDLIIERSDGYPTYNFAVVIDDALMNITHVIRGDDHVNNTPRQIQLYEALGF
                     DVPKFAHVPMILGSDKTRLSKRHGATSVMAYKELGFLPEALVNYLVRLGWAHGDQEIF
                     STAELIERFSMDAIGKSPAVFNPEKLLWLNAHYIKEASVERLMEEMKPLWPSVIDTGN
                     TDFIRKVIADFQPRVKTLMELVQSSDFYFADEIHYDKEAAQKFLTRDIVDHMKAIANT
                     ISSVQQFSKESLEEFFKTFTQTQNIKFKLIAQPLRVALTGKTVSPGIDEVMVTLGKER
                     VIKKINKAIAYIESLA"
BASE COUNT         3827 a         3739 c         3346 g         3816 t
ORIGIN      
        1 gccgattcgg ccgcgctgtt gctggcgcag gacaaacagc gggtgaaggc cctgctggat
       61 cagtgcgcca ttcccacgcc tgcatggaaa atcttcgatg cgcctgaccc tcagggctgg
      121 aaaatttttc cggcaatcgt caaacctcaa aacgaacatt gttccgcagg catcacgccc
      181 gagtctgttg ttctgcaacc gacggatttg caaaaacgca tcacctttgt gctcgatcat
      241 tatgcgcaac cggcgctggt ggaagacttt attgacggac gggagtttca tgttgccgta
      301 tggggaaacg aaaccgtcaa cgtcctgccc gccgcagaga tggacttttc ccgcttcgga
      361 gacctcaatg accggctctg cacctacgag gcaaaattta ctcccgggtc gctgcattat
      421 gaagagatca aaacccttct gcccgcccca ctgaccacgg gtgaaaaaaa gtcgctggaa
      481 acggtttgcc gcaacgccta tcgggcatcc ggatgccggg attatgcccg catggatata
      541 cgcttgcggg acaatatttt ttacgttctg gatgtcaatc ccaatgcaga tatcagttcc
      601 gatgccagca tggcctgcgc tgcggaagcg gatggaatca gttatgccga aatgggcagc
      661 cgcatcgttg agatggccgc aaaaaggcat ccggtccttg ggaaactctt ccggagttga
      721 cttttaacag gcgatggttt agacatacgc atattcatta agggagtcgc cttgtcatga
      781 acaaaaaaga ttttctcaaa accaagatcg aacacatcga tattaaaaaa ataaacgcgg
      841 tggacatcgt cagcgcgatg aaaaaaatgt ccttttccgc ccgcgatctg gccaatgccg
      901 ccgatatata cgaccgtatg ttgaaagaaa aaaactgcgc ggtgatcctg acactggccg
      961 gttcgacatc cgccggcggc tgcatgcagg tttatgtcga tctggtgaaa aacaatatgg
     1021 tggatgccat tgtggccacc ggcgcatcca ttgtcgacat ggattttttt gaagcactgg
     1081 gattcagaca ttatcagggc acaccgtttg tggacgacaa actgttgcgc aagctctaca
     1141 tcgaccggat ttacgacacg tttattagcg aaaacgattt gcaggcctgc gacaagacca
     1201 ttaaaacaat cgcggatggc cttgcgccgc ggccttattc ttcccgcgaa ttcattgccg
     1261 agatgggcag gtacctcaca aaaaacgcca aaaagaacaa ttctctggtc caggcggctt
     1321 atgaaaacaa cgtgccggtc ttctgcccgg cattttcgga cagttccgcc ggtttcggcc
     1381 tggtcctgca tcagcatgaa catccgggaa accatatgtc gattgattcc gtcagggact
     1441 tccgggaact gaccgatatt aaaatcaaag cgggaacaac cggcttattg atgatcggcg
     1501 gcggtgtccc gaagaacttt gctcaggata ccgtggtttg cgcggaaata ctgggctacg
     1561 atgtccccat gcacaaatat gccattcaga ttaccgtggc ggatgtgcgc gatggtgcct
     1621 gttcaagctc aacgctcaag gaagccaatt catggggcaa ggtcgattcg tcgtgcgaac
     1681 agatggttta cgccgaagcg acaatggctc tgcccttgct ggccagttat gcgtaccaca
     1741 aaggcaactg gaagaaacgg aaaaaatgga atttatccag gattttcaaa taaatatctg
     1801 cctgctgtat tcaatagttt tttaatgcat tggtaacttt tacgggcgga ataaccgtca
     1861 ttatggggaa aataaccgtt ttttctcaaa acgatttagg gccccgtacg gtcagacagt
     1921 ctccgttaag ccattagtta ttaattttat taaatattat cttaaaactt gccgcgctgc
     1981 cctatcactc atttaaggcg acattgggca cataaattgc ttaaagtcat agaatatgca
     2041 tcagcttttc ttcatgaata cgcgattggc gtgatcaatt gcattggtca gctcatcacc
     2101 gaaaccggca acgtaacttg aataacgatg gaagacaaaa acgaacgaac aaaattaaca
     2161 gaaagattgt tttcttctga agacaacaat cttccattac tggcaaaaga tattatatcg
     2221 aatgccggcg tagcaattta tatcgttcac aacagtcaat ttgtttacgt cagcgaatta
     2281 taccagaaac tcaccggcta cgcggaaaaa gaacttatcg gggaatattc cctcaaaaat
     2341 attcatcctg atgacaggga taaagtcagg aaacaggcca ttaaatgcct gaaaaaagaa
     2401 agttttgagc cctacgaata cagatatatc cgcaaagata atgaaatcag atacattctg
     2461 gaaacagtgg cgccgattat ccataaaggc gagcgggcga cactgggcag ttttatggat
     2521 atcacggaac gcaagatcat cgaagaaaaa tacagaaaca ttctggaaaa tattcaggaa
     2581 ggttattttg aagttgatct tgaggggaat tttacttttt ttaataatgc cgtctcccgt
     2641 gttcttggat attcccggga agaattgatg ggcatgaata accggcagta cacacccgac
     2701 aaggactccg cgcgaaaagt ttttcaggct tatcatcatg tttacgaaac aggtgagccg
     2761 ctgaatgcag tcggctggca catcattacc aagcaaggcg ccaaaagata tattgaagga
     2821 tccatttcgt tactcaagga ttctcaaggc aaaccaatcg gatttcgcgg tatcacccac
     2881 gatgtcaccg aacgtaaagt ggccgaagaa gcgctgcgtc aaagcgaaga aaagtacaga
     2941 aacattctgg aaaacattca ggaggcctat tttgaagttg atcttgcagg caattttatc
     3001 ttctttaacg attcgctgtg tcgcatcaca ggaggaacaa gagaagaatt aatgggtgcc
     3061 aattacacgc ggttttccga tccggcaacg tcaaaaaatg tgtttctggc attcaataaa
     3121 attttcaaaa caggagaggc cacggaggga ttcgactggc tgattatcag aaaagatggt
     3181 tccaaaaaat atattgaagc gtccgcatcc ttaaataaag atgcttcggg aaagcccata
     3241 gggttccggg gagtcatccg cgatattacc gaacgcaagc gcattgaaca ggaactcaat
     3301 cacatggcca cgcacgacgc gctcaccggc ctgccgaacc gcctgatgtt cagccagttg
     3361 ctggatcacg ccatccaatc cgcgcaacgc aacaaaaaac acctcgctgt tttcttcatc
     3421 gatctggatc gcttcaaaat cattaatgat tccctcggac acgaagccgg agacactctg
     3481 ctcaaagaga tggctttgcg gttcaaacag tctctgcgta ccgtcgatat tatcggacgt
     3541 ctcggaggcg acgaattcac gattctggtt gaagatttta acgatctgaa gcaggtggaa
     3601 tttctggcca agaagatttt gaacaccgtc atgcaaccga ttgcgctcat gggggaagaa
     3661 tgtcgtgtca ccgccagtat cggtatcagc gtcttcccga aggacggact ggatgaacag
     3721 tccttaatga aaaatgcgga cattgccatg tactttgcca aatcggaagg aaaaaacaat
     3781 ttccagtttt actccgaaaa tatccgctcc atgtccaacg aacggctgac catcgaaacc
     3841 aacctgcgtc atgcaatgga acgcaatgaa ttttacctcg attaccaggc aaaactggat
     3901 ctgcagacca gggcaatcac cggcgtcgag gcgcttctgc gctggaataa tccgtatctg
     3961 ggactggtcg cgccgacgca gtttattccg attgcggagg agacaggcat gattataccc
     4021 atcggccgat gggtgatcaa aaccgcctgc atgcagaacg tcgcctggca gaagcaggga
     4081 ctgccgcccg tctgcatgtc ggtcaacctg tcgcttcgtc aactcatgta tgaaaacttt
     4141 gcagaagatg tcaaaaccat tctgaatgaa acgcgcatgg cttcggaact actggagctg
     4201 gaaatcaccg aaagcacggt catgcacaac cccaaacgtc ttgtcccgat cctgtcaaaa
     4261 ttaaagaaaa tgggaatacg gatggcaatt gatgatttcg gcacgggtta ctcttcgctt
     4321 gcacagatca aaaacttccc gatcaacacg ctgaaagtag accgatcatt tatccgcaat
     4381 cttccgcagg ataccgataa taaagccatc acggaagcgg tgatttttat gggcaaatcg
     4441 ttgaaactca aagttgtcgc cgaaggcgtg gaaaccaggg aacaggcgaa atttcttaaa
     4501 gatcagatct gcgacgaaat gcagggcttc tatttcagca aacccatcgg gcctgacggc
     4561 tttgccgatc ttcttcgcga aaattctgtt tcgggaacag atcccggccg tcaataattt
     4621 ttttgtaaaa aacaaccggc attattgtgt catctgcaaa aatcagtttt tcgccagaca
     4681 ttcttccagc ggggaaatat ccacggatcc cttgtcgttg acattgtttt tgaattgtct
     4741 gacgccttga gcgaccgaat gaagattcga aggtgaggtg actgcacagg cgttcatttc
     4801 cgagagtttt tcgatatcgg atgccagaaa atcaaaaatt ccggtcaccg ccgcctccac
     4861 aatatcggac gtgacgagat ttgcgcgtcc ttctccgtac agatcaaacg caaagctttt
     4921 tatggccatg ctgtcgataa tctgagttac ggtatatgcg acgtcataaa ttctatcata
     4981 cgtgatatcg ggaacacgac caagtccgaa taataacagg atttccgaac cgatcctttc
     5041 cggaaaagga ataacaactt tttccttaaa accgccgccg attcttccat ttttgatttc
     5101 ccgggaaagc atgccattga gacgccagtc gatcaaaccg ccgatgccgc gcggcggctt
     5161 ttcgtcttca aagatgccga gcgccaaaca tttatgtttt ggaagatcgg ggttttctat
     5221 cgagagactt agctgcattt tttttattta atttcagatt gagagcgtcg agtatgccgt
     5281 tgatgaacga tccggagttt tccgacccga agatttttcc cagatcaatg gcctcattca
     5341 tcgtgacctt cggaggaata tcatcacagt agagcaattc aaatacggca agacgtaata
     5401 tgttgatatc cacgcgggac attcgcccca acgaccagtt atccgaacat ccggcaatga
     5461 gttgatccag ttctttcctg tggtcgcatg ttcccatcac gagaaaggcg gcaaaatcct
     5521 tggcagattt tggtgcaaca aaattacccc agaaaagatc cagggcttcc tgcgccggaa
     5581 tatcgacaaa attaagactg taaagaacct gtaaggcaat ttctcttgcc tttcgccgcc
     5641 cgcacatcaa ttttcaacct caaagaacca gaacatccga tcatttctgt actgttgccg
     5701 tcagaaaaaa cggcattgca acatcaaatt tttttgaaca aatccaccat ctcaatggct
     5761 gtcatcgctg cgtcggcccc tttattgccc gacttcgttc ccgcacgttc gatagcctgt
     5821 tcaatggtgt ccgttgtcaa cacaccgaaa acaacgggca cttccgtttc aaggccgaca
     5881 acggcaatcc ccttggagac ttcggcactg atatattcga aatgcggcgt tgcaccgcgg
     5941 ataatggccc ccagacagat gacggcatca aatcgaccgc tcttggccag tttctttgcc
     6001 gtcagcggca gttcaaaaga gccgggaact ttataaatga cgatatcctt ttcttctgca
     6061 ccggccctga tcagcgcgtc gatagtccct tcgattaatc tgccgcatat aaaatcattg
     6121 aaacggctgg ccacgatgcc aaacttcatt ccctttgcaa cggtctttcc ttcaattatt
     6181 ttcagcatag ttcttaacca tccttacgtt ttattttagc cttgcggtgt aaaatctata
     6241 ttttcaaaag gtgccccatc ttttttttct ttgttttcat gtagtgaatg ttgttttcat
     6301 tgggatcgat ttcgatggga acgcgtttga ccacttcgat accgtatcct tccagaccga
     6361 caatcttctt cggattgttt gtcaggagac gcattttgcg cacgcccaga tcagccaaaa
     6421 tctgagcacc gatgccataa tcgcgtaaat ccggtttgaa tccgagttcg atattggcct
     6481 ccacggtatc cttgccctgt tcctgcagag cataagcttt gatcttgttg accagtccga
     6541 tgccgcggcc ttcctggcgc atataaacga taatgccctt gccttctttt tccaccatgt
     6601 tcatcgcacg atgaagctgg tcgccgcagt cgcagcgcaa cgacccgaaa agatctccgg
     6661 tcaggcattc ggaatgaacc cggaccagga cttcgtcatc ttctttaata tcgcctttca
     6721 ccagggcgat atgctgccag tcgtcaactt cattttcata aacgatcatt ttaaaatcgc
     6781 cgccgaaact ggtcggcaag gtcgcttccg ccatacgttt gataagcgat tcattctgca
     6841 tccggtaatc gataaggtcg gcaattgtca ctatttttaa cttgtgttcc ttggcaaata
     6901 tttccaaatc cggcatccta gccatggtgc cgtcatcctt cataacttca cagatcacgc
     6961 cggccggtgt caaaccggca agtcggcata aatccaccga cccttccgtc tgccccgtcc
     7021 ggaccagaac cccccctttt ttggcacgga taggaaagac atgtccggga ctgaccaaat
     7081 catccggttt ggcctccgga ttcacagccg cctgaatcgt tgttgcccga tccgctgcgg
     7141 atataccggt ggtgacgcca tggcgcgctt cgatggaaat ggtaaaagcg gtgccgaaac
     7201 ggcactgatt atcctgaacc atttgtttca gatgaagctt atccgccata tcttcattaa
     7261 gggtcagaca aatcaaaccg cggccgtagc gggccataaa gttgatggtt ttagccgtgg
     7321 caaattgcgc tgccatgcaa agatcccctt cgttttcacg atcttcatca tcaacaagaa
     7381 tgatcatttt cccgtttttg atatcttcta ttgcttcctg tattgtgctg attgccattc
     7441 gtctttcctt tattccttaa aaaccgtgtt tcacaaggaa atccttatcg atccccttat
     7501 ttgattgcaa taagttttcc acgtaccggc ctaaaatatc cgtttcaata ttcacccact
     7561 ccccttcttt gcgggttacc agggtggtat tgaccgccgt gtgcgggata atattaacat
     7621 aaaaccggct tttttcaagt ttgttcaccg ttagactgat gccgtcaatc gccaccgaac
     7681 ctttttccac aatgtagcgg gaaatcgtat cattcatctc gacagcgata atcagtgaac
     7741 ccgatttttg catccgggac aggattcttg ccgtggcatc gacatgcccc agaacaaaat
     7801 gtccgccgac aaaaccgcca acacgcagcg atttttccag attcacctta tgccccgcct
     7861 gtaacaattt aagcgtcgtt cgggacagcg tctccgcgga aacatccgcc tgaaaattat
     7921 tttgctgcag ggaggtgacg gtgaggcagg ctccgttcac ggcaatactg tcgccaagcg
     7981 aaacgtcttc cagactgatg ccggcgtcaa tgtccagaac cgcatctgcg cccttcatgg
     8041 tcaaacgcct caccttgccc agtccttcta cgattccggt aaacatgatt actttttacc
     8101 tttgcttaaa agatccttca gctccgacat gaactcaccg acatcgcgaa actgccggta
     8161 aacggacgca aatcggacat aggcgacgcc gtcgaaattt ttcaactcgc tcatgatctt
     8221 ttctccaatg accgtggacg aaatttcttc ggcctgcatt tcctgacagg cctgttcaat
     8281 gttttcgaca agtttgtcaa tcgcctccat gctgatcggt cgtttctcgc aggctttttt
     8341 gatgccggtc agaattttct gccggtcaaa ctgctcgcgg cgaccgtctt tcttcaccac
     8401 catgggcaga acgtcttcca cgtattcata ggtagtgaaa cgcttggtgc accccaggca
     8461 ttcccggcgc cggcgaacgg ctttcccgtc cttgctgatt cgagaatcaa tcaccttatt
     8521 ttccgtgtga ccgcaaaaag ggcatttcat gatgatgtca ccttatgtgg ttaaagttgc
     8581 tctacgcgga tccccgcttc tttgagcatc tccgcagcca gttgatcatg ataaccgtca
     8641 cggatgacga cacggatgat cccggcatta atgatcattt tggcacaaat gacacagggg
     8701 tgattcgtgc aatacagcgt cgatcctttc gtgctgacgc cgtgcagcgc cgcctgaata
     8761 atcgcattct gctccgcatg cagcccgcgg cataattcgt gacgttcgcc ggaaggaacc
     8821 ttcaactgtt cccgaagaca gccgatatcg agacagtgct gaagcttgga gggagcgccg
     8881 ttgtatccgg tggccagaat tcggctgtcc cgaaccagtc cggcccctac cgatctccgg
     8941 caacaggtac tgcgcctgga aacaaggtcg acaatatcca gaaaataact gtcccagtcc
     9001 ggtcgttttt ttttcttctc ccgcaaaacg gctgctgacg attttatttc catcataatc
     9061 gctttcgatc aaaaaaaatg gtccgtgttt taatttttca gccgcccctg atacagcgga
     9121 aaacgatcgc aaaggacttt gatcttctgt gcaaccgatg tgattttctg ctcgctactg
     9181 atgtttttaa tcacgtcagc aatacacgac gcaattaaac gcatttcatc ttccttcatg
     9241 cctcttgtgg tcacggcggg cgtgccgatt ctgataccgc tggtcacctg aggccccttc
     9301 gtatcaaagg gaatgccgtt cttattgacg gtgatggagg cgcgatccag agcgtcctgc
     9361 acgtcctttc cggtgacatt cttgtcggtc aagtcaacca gcatgagatg attgtcggtg
     9421 ccgccggaca ccagccggaa tccctgattc ctcagttcat ccgccatcgc ctgagcgttt
     9481 ttaatgatct gtgcctgata ttccttgaat gccggctgaa gcgcttcctt gaaggcaacc
     9541 gccttggcgg caataacatg catcaacggg ccgccctgca tgcccgggaa aactctgctg
     9601 ttaagtgttt ttgcgtaagc ttccttgcac atgaccacac cgccgcgcgg tccgcgcaac
     9661 gttttatgcg ttgttgtagt gacaaattcg caaaccggaa ccggagaggg atgcaatccg
     9721 gtgcaaacca gaccggcgat atgcgcaata tccgccataa tcacggcgcc gacttcatcg
     9781 gcgattttgc ggaatttatc aaaatcgatg gttcgcgggt aagcactggc gccgacgata
     9841 atcattttgg gcttgtgttc cttggccagt ttttccactt cgtcaaaatc aatggtttct
     9901 gtttcccggt tcacaccgta ggggacaatc ttataaaact tgccggaaaa gttggccgga
     9961 ctgccgtgag aaagatgccc gccgtgagac aggttcattc ccagaatggt gtcgcccggc
    10021 tgtgcgacgg caaaatacac cgccatgttc gcctgtgttc ccgaatgcgg ctgaacattc
    10081 acatgatcgg ctccgaaaag ttttttgcag cgctcaatcg ccagactttc cgcaatatcc
    10141 acgtattcac agccgccgta gtaacgtttt tccggatacc cttccgcgta tttgttggtc
    10201 atcacggagc cctgcgcttc cagcaccgct tcactgacaa aattttcgga ggcaatcaat
    10261 tcgatttttc ccgcctgacg gcgtgtttcc agatcaatgg ccttcattac ttccggatca
    10321 actttttcta aaaaagacat gaaaattttt ctctccttcg ttctaaattc tgcttattgc
    10381 accatccccg tttcgcttca caccagggat aatttccaat cccgatatat cggaatggga
    10441 tcattcgtcc aacaaatttc agcgagctac gctttatgaa attgggactc attaaatttc
    10501 ttaaataaca aaaccgcatt gacgccgccg aaaccgaacg tattggacat ggccgtatgg
    10561 attgcctgac gacacgccgt gttgggtaca taattcagat cacacccttc accgggattg
    10621 tcgaggttta tcgtcggcgg aataacgctt tcctgcaggg atttcactgc aaaaatcgct
    10681 tccacgccgc ccgcggcacc cagcatatga ccggtcatgg atttggtaga gctgaccaac
    10741 aatgttttcg cgtgatcccc aaatagagat ttaatcgcct gcgtttcgta taaatcatta
    10801 agaggcgttg atgttccgtg cgcattaata taatcgatgt cttcgggatt cagaccggca
    10861 tcctgtatcg caacccgcat acagcgggca gcaccttcat gaccgggtgg cggtgcagcc
    10921 atatgaaaag cgtcagaggt acaaccgtat ccggccagtt ccgcataaat tcgcgcccct
    10981 ctgttgagtg caaaggataa ctcctccagg atgacaatgc cgctgccttc gccgatgatg
    11041 aaaccgtcgc ggtctttgtc aaacggcctg ctggctttct gaggatcgtc gtttcttacc
    11101 gacagcgccc gcatggatcc gaaaccgccg acggccagtt gcgtcaccgc ggcttcaact
    11161 cctccggcaa tcatgacgtc ggcatcgctg taggcaatgg ttttgtaggc atcaccgatg
    11221 gccgatgttc ccgacgcaca ggctgtcaca gcacaattga tgggaccttt ggccccgaat
    11281 ctgatggaca cctggccgga tgcgagatta gcaagaatgg caggaacggc aaagggagaa
    11341 attttgcgcg gaccggaatc aaggagattt ttaaattctt cttccagagt cgccagcccc
    11401 ccgatggcgg aaccgatgat cacacccgtt cgttcggaaa tatcggaacc aatcgtcagg
    11461 cgggcatccg ccagcgccat ctcggaagcg gcgatggaat aaagaaggaa gttatcataa
    11521 cgacgggctt cctgtttgct gacatattgc agaggatcga aattttttat ttcaccggca
    11581 atttttgttt taaacgcggt gctgtcaaat tttgtgatca ggccgatgcc cgacttaccc
    11641 gcaatcgcag ccccccatga ttcatgtaat gaatttccca agggggtcaa cgcaccaaga
    11701 cctgttacga ccacacgtct cttcatgggt ttaagtcctg cacggctttt tattgaacgc
    11761 ctttctgtct cagaaaatca accacgtctt gaatcgtggc aattttttcc aactcctcgt
    11821 cagctatttc tataccaaaa gcctcttcca tttccataag gagctcaaca atatccagag
    11881 aatccgctcc caaatcgtca ataaaagatg cttccaaaac gcattcttcc tttgtgacgt
    11941 caagctgctc cacaataata ttaatgactt tttcttctaa cgtcatgcac catcctcctc
    12001 acgtgtatga ttgcttacca atttggatcc atgtgcttct tttatcatcg ccctccggga
    12061 atcattctgg ttccggttta aaagaagact atttgctcac caatggatta cagacacatt
    12121 ctgctctgtt tgaagttttt caaaatgact ttttagtcat tcttttccgc ttttctgtat
    12181 tctctgcctt tgtacgtttc gcaggtcgga caaatgcggt gaggcatctt gggttcattg
    12241 cactgtgggc atacggacat ggacggcgat ttcaaaaaat cgttcgccct tcgctggttt
    12301 cgtctggttt tggaatgtcg ctttactgga tttggcataa ttcacctttt tctataatta
    12361 gttttttatt ttaaaatttt ttaataccgc catacgggtg tccctgacat ctttttcgca
    12421 ctggcaggac gcggcattca aattggtgcc gcaacgctga cacagccctt tgcactcttc
    12481 gcggcaaaga attttaatcg gtatctgcaa aatgatttgt tcacagataa tgggcgccag
    12541 gtcaataaaa tctccctggt aactgctgat ttccaattct tccggtttaa gttctatctc
    12601 ctctttttgt tccgctttca ctggaatcaa ggtataggta aagttactgc cgatggtcag
    12661 cctggttctt tccagacaac ggctgcagtc ggcatcaatc agcgctgaaa agtttccctt
    12721 gatgaaaaca gtgcctgacg tttttgtgat catacaatcc acatcaatgg tttccaaagc
    12781 aaaatcaagg cgttcttcct tgtgaaagca cgttttaaac caggcatcgc cttcggaaaa
    12841 aacaaagcgc tgtccttctt ccggaatcag caaaacgtta attttcaaag aattagacat
    12901 agatagcccg ttgtctgaaa aatatcttaa atttaacctt gcgagtaaat tataaaatct
    12961 tttcattgtc aagtataatt tattgactcc ggttatgcaa taagcattta attttatatt
    13021 tgatttcaat tctaaacaat cgcgcggccg gatgaaatca taaatatttt catggtgctt
    13081 gacaaaagca tgctctcaac tctataaggc tataaaaaac aggacataaa catgcaaaaa
    13141 atcagaactc gcttcgcccc ttctcccacc ggttttttac atatcggcgg ggcaagaacc
    13201 gctcttttca actggcttta tgcccgccat cttggcggcc agtttattct gcgcattgaa
    13261 gatacggacc ggcagcgctc cacggaagaa tcaacaaaag caattctcga tgccatgtcc
    13321 tggctggggc tgaactggga cgaaggaccg cattttcagg cccaacgggt acaactgcac
    13381 cgggacatgg tgaaaaaatt aatcgatgaa ggcaaagcct attactgcac ctgttcgccg
    13441 gaagaactcg agattaagcg caaacaggcg ctggctaacg gtaagaaacc gaaatacgac
    13501 ggcacatgcc gtaatagaag cctggcgaaa tcggcaaaca gtgtggtgcg cttccgctcc
    13561 cgagacgacg gcgcaaccgt tgtggaagat atgatcaaag gaaacatcat ttttaataac
    13621 gaagagcttg atgatttgat tatcgagcgc agcgacggct atcccacgta caattttgcc
    13681 gttgtcattg atgatgccct gatgaacatc acccatgtca tccgcggaga tgatcatgtc
    13741 aacaacacgc cgcggcagat ccagctttat gaggctctcg gttttgatgt cccaaaattt
    13801 gcccacgtcc cgatgatact gggaagcgat aaaacgcgtc tgagcaaacg tcacggcgcc
    13861 acgtcggtca tggcctataa ggagctgggt ttcctgccgg aagcgttggt taattatctt
    13921 gtacgtctgg gttgggctca tggcgatcag gaaatttttt cgacggcgga gctcatcgaa
    13981 cgcttcagca tggacgccat cggcaagtcg ccggctgtct tcaaccccga gaaactgctc
    14041 tggctcaacg ctcattacat aaaagaagct tctgttgaaa gactgatgga agagatgaag
    14101 cccttgtggc cgtcggttat cgataccgga aacacggatt tcatcagaaa agtgattgcc
    14161 gattttcagc cgcgggtcaa aaccctgatg gaactggtcc agtcgtccga tttttacttc
    14221 gccgatgaaa ttcattatga caaagaagcg gcgcaaaaat ttctgacgcg ggacattgtc
    14281 gatcatatga aggcgattgc gaatacgatt tcatccgtgc aacagttcag caaggaaagt
    14341 ctggaagagt tttttaaaac atttactcag acccaaaata taaaattcaa attgatcgcc
    14401 cagccgctga gagtggcctt gacgggaaaa acggtcagtc cgggtattga cgaagtgatg
    14461 gtaacgttgg gtaaggaacg tgtcattaaa aaaattaata aagcgatagc gtatattgaa
    14521 tcgcttgcct gatccttatc gccgactcag agcggtccaa ttttaacttg acttttacag
    14581 gctgattcaa tagatagtca agcctcaacg aggcaggaat accgcgaaaa acgagcgttg
    14641 agtgcatccg ctcagaaaca taaaaacatg ccgccatggt cccatcgtct agtggttagg
    14701 acgctggcct ctcacgccgg aaaccggg
//
LOCUS       DAOOLB010000046        14463 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4492_length_14463_cov_41.818226, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000046 DAOOLB010000000
VERSION     DAOOLB010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14463
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4492_length_14463_cov_41.818226"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="PLF77_06960"
     CDS             complement(<1..248)
                     /locus_tag="PLF77_06960"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPC08628.1"
                     /translation="MTQVYQYKLTVPESAVDINGHVNNVEYLQWMQWAAVLHSDIRGC
                     TKATLDVGATWVVRMHHIEYLRPAFAGDTVVILTWVSD"
     gene            409..2241
                     /locus_tag="PLF77_06965"
     CDS             409..2241
                     /locus_tag="PLF77_06965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPC08629.1"
                     /translation="MVKEKESNVAGNLESYYTKPNNLVDMFEDSAAKFASRNLFGTKN
                     KETNQYEWVTFAQVAERVNNLRGALKKIGFEKGEKVGVIVGNSAEWFVCANATHGLGG
                     VFVPMYEKELQKVWQYIIKDSAIKYLFVSNQKIYEIIKNFQNDIPTLKGVFMLYGSGE
                     NSLAELEKMGKANPVLSYKPHWSETAVIIYTSGTTGDPKGVLLSHGNLTHNAKESHWT
                     FDVGEEEVGLSILPWAHSFGLTTDLHVFVYGGWQLGLAESADKLLLNFGEIKPTHMNA
                     VPRVFNIIYDKIQQSVSADPEKKKIFDAAVAEAIKNRDLKEKTDEFKFYDAVAFSTVR
                     NIFGGRLKQVVIGGALMKPEIAMFFSDVGVVPYVGYGLSETSPVLTNNSPKRGNKFGT
                     IGKPYKDTKVVIDKSRVGEDSPDGEIVVYGAQVMQGYHNKPETTKEVMMPDTWNGFPG
                     VRTGDRGWIDEDGYLNITGRFKDEYKLENGKYVHPESIENEIKLLRYVSNVIVYGEGR
                     TFNVALVVPDFEAVKTDSQTSRWAQGTPDETIANKECTDFISKQITDHLRKSFGGYEI
                     PQKFLYITEDFSVDNGMLTQTLKLKRRNVMEKYGERLRALYDNV"
     gene            2311..3522
                     /locus_tag="PLF77_06970"
     CDS             2311..3522
                     /locus_tag="PLF77_06970"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPC08630.1"
                     /translation="MNNQNDVVIVSAVRTPFSKFGGALKEIHSTDLGVIVINESLKRA
                     GMTGEDLDEVYYGMCDQAEAALYDNVNARQAILRAGLPSTLVSLTLDRACCSSLCAVQ
                     LGRRAILLNEADACMAVGAENMSNTPLLINGHRWGSGMKPLLVQDYLNPITYREYTPL
                     GRDAGEIAIEHGISREEQDAWALGSHQKWDAADKAGLFYDELVSVELPAKKGTPVIFG
                     KDELPRPDTTLEALARLKTIYGSPTVTAGNAPGLDAGATAVIIMRRSKAEKLGIRPLA
                     TIVAVASGAGEPRRMAELPAKVIAQALKRASLCIDDMSVIEINEAFAAVTLVSTKMLA
                     GGDEAKWRELLKKTNPNGGAIAIGHPVGASGGRILMTMMYELKRRGGGYGICGICGGL
                     AQGDAVVIRVD"
     gene            3615..>3828
                     /locus_tag="PLF77_06975"
     CDS             3615..>3828
                     /locus_tag="PLF77_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08631.1"
                     /translation="MRDEKGIASWVVIVIIVLLIAAGFLWSKYFAAPTATVMMQGADQ
                     AKPAIEKAHQASDAADRANKIAEEAAK"
     assembly_gap    3829..3928
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3929..4913
                     /locus_tag="PLF77_06980"
     CDS             <3929..4913
                     /locus_tag="PLF77_06980"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPC08632.1"
                     /translation="VMKKALSFVWILMLLAFATVAQAEVKAGSFSVTPFIGGYVFEGN
                     EYDNNEDTYAAGLRAGYNFTKNIGVEAFFNYVPTNNKDWNDDNEMELFGYGLEGLYHF
                     MPESRFVPFFAVGVGGIHYDALPEDPDNRDKFALDYGAGFKYFVTDNIALRADVRHII
                     PFNERYNNLLYSVGLTFAFGGEKKIVETKVEEPLPPPPPPAPVAKPVIIEKGRQTLDV
                     KFDFDKATIQDGYYKDIDALVEVMKDYPELNVVIEGHTDNVGKAEYNKKLSQKRAETV
                     KKYMVEKGGVDAARIEAKGFGDERPVDTNDTADGRAKNRRVEAAVDYIIEK"
     gene            5348..6358
                     /gene="aroF"
                     /locus_tag="PLF77_06985"
     CDS             5348..6358
                     /gene="aroF"
                     /locus_tag="PLF77_06985"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HPC08633.1"
                     /translation="MIIVMKSHATEKQIEDVIHWIESVGYKAHPSIGVERAIIGAVGD
                     NRGKDILKFAESLPGVEKTMPILKPYKLASRESHDGNTEVTVGEVTVGGPEFVVMAGP
                     CAIESEEQLLESAYVVKKGGAQILRGGAYKPRTSPYSFQGMEEEGLKVLNKVGTQAGL
                     PTVTEVVNPADVDLVESYADMLQIGARNVQNFALLKKVGHSRKPVLLKRGMMTTIEEL
                     LMSAEYILSEGNPNVILCERGIRTFETATRNTLDISAIPVLKGLTHLPVIIDPSHAAG
                     NWKYVIPLSRAAVAVGADGIIVEVHPEPEKAVSDGAQSLKPEKFYQMMDEIRAITAIM
                     KH"
     gene            6389..7483
                     /gene="aroB"
                     /locus_tag="PLF77_06990"
     CDS             6389..7483
                     /gene="aroB"
                     /locus_tag="PLF77_06990"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPC08634.1"
                     /translation="MRSIKVNLDRKSSSSYEIRIGKDISDRLSLLIAKNHKAARYAVI
                     TDNIVGELYGKKFLTALKEIGLNASLIDFPAGESSKTIKTVVDIAETLAVSGADRETC
                     LIALGGGVVGDIAGFVASIYMRCVPYIQIPTTLVAQVDSSIGGKTGVDLPCGKNLAGT
                     FYQPQAVMTSLHFLNTLPPKEFNNGLAEIIKYGVIDDEKMFASLEDIMTAVKSKDPKI
                     LLGVVEKCCQIKKSIVEIDEKEGGVRRILNFGHTIGHSLEAVSGYAITHGEGVAIGMV
                     AAARLSARMGYLKNNEAGRIETLIDQAGLPTRIPKTLSGGKIVAQLKMDKKRKGAAIH
                     FVLLKKIGLPFIHGNIEQKMIADVIEDMKS"
     gene            7480..8562
                     /gene="pheA"
                     /locus_tag="PLF77_06995"
     CDS             7480..8562
                     /gene="pheA"
                     /locus_tag="PLF77_06995"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HPC08635.1"
                     /translation="MKPHLLENLRKKIKDKDKEIVRLLNERAQISVQIGKMKGQGGMQ
                     VYDPAQEAKVHGYLRDLNAGPLASGAVSAIFREIISASRDLQKPMTIAFLGPEASFSH
                     LASRLHFGESSLFFPQTGIFRVFDEVEKGAIDWGVVPVENSLEGSVNVTLDRLMITPM
                     KIRAEIYLRIRQCLMSSAKNMKEVKRIYSHPQALAQCQVWVRTNLPNCVVCETESTAA
                     AVQMIRGKKHEAAIGSSLAAKIYGVNLLAEGIEDNPSNMTRFLVIGQGANDPTGNDKT
                     SLIFATPHSPGALHAALSAFARRKINLAKIESHPVKDKLWEYSFFVDMIGHADDRRVQ
                     GCLRELQNKTSFMKILGSYPKSEDAL"
     gene            8559..10133
                     /locus_tag="PLF77_07000"
     CDS             8559..10133
                     /locus_tag="PLF77_07000"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001319.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPC08636.1"
                     /translation="MICIPITSDNPKEALHAIERSCQLADFLELRMDLIGNIGLAELI
                     SAVRAESAAVKIIVTSRKKEEAAFVGRWPRRRNACSKTRYKIALLKEAVERGADYIDI
                     ELAEGTQAIRQLQALCAEKGSQTKMIISYHHLKDTPDLATLKKIFHRCAAYKPAVVKI
                     VTTARSEEDNLIILNLIAYARRKSQKIISLCMGEKGGISRAVAPLMGNFLSFAALDRT
                     GRSAPGQFTVHEMNRIQKWFKGKNRAPQSSASSPHASLPRHYVLLGNPVEQSLSPVMH
                     DAALKKTGLAEQYVAFCVQDIGGAMDGLRAMNIHGASVTIPFKVAVMEYLDDIDADAL
                     EIGAVNTIVNENGRLIGHNTDWAGLILTLKKAMTIRHKTIVIVGAGGTARAAAYGILK
                     EGGCPVIVNRTPKKGKMLAEKMQCAFYPLSEIGRIRGDVLINTTPVGMYPRGNQSPVS
                     AAVLSDYDCVMDVIYNPIKTKLLRDAEKQGCRIVTGVEMFVNQGAEQFRLWTGKEPPH
                     AVMKKIILERLSKGGR"
     gene            10123..>10469
                     /locus_tag="PLF77_07005"
     CDS             10123..>10469
                     /locus_tag="PLF77_07005"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08637.1"
                     /translation="MDDNPIRCPDPAVTVRVPGSKSLSQRALVAATLAQGDSMISNLL
                     VSEDTLYMIGALRELGAKIVDAPDGFQVTGTAGKIENSGRDLFLGNNGTALRFLTALV
                     CLGKGQYVLTGEK"
     assembly_gap    10470..10569
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10570..11208
                     /locus_tag="PLF77_07010"
     CDS             <10570..11208
                     /locus_tag="PLF77_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08638.1"
                     /translation="GVTISKPERFAYRVNAGEPYRGRRYVVEGDASSASYFFLAAALL
                     KTTIRVEGISRESRQGDIRLLDVLEKLGCRFRSTTNGVDVTGGTLLKGDRVFDFGDMP
                     DMVPTLAVLSAFREGQTVINNVAHLRIKESNRLAAVATELRRAGIDAREIPDGLVIKG
                     GRMRPAKIETYNDHRMAMSFAIAGLVVPGIDIRDKKCVDKSFPSFWEELRRL"
     gene            11205..11777
                     /locus_tag="PLF77_07015"
     CDS             11205..11777
                     /locus_tag="PLF77_07015"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPC08639.1"
                     /translation="MKVVLIGYRATGKSTVGKMLAARLNVDFRDTDAMIEESMDMPVK
                     EIVALHGWEYFRSKERDIIKSLVKKEDCVIATGGGVVLFRENVDLLKQNVDLLKQASV
                     MVWLNAPLRDIVERLKQDAQNEATRPPLTSGNIVQETIDTMRKRFPLYERSADYVVDT
                     LSKTPEQITEEIYQYLLKSGNLAKITVIKK"
     assembly_gap    11851..11950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11951..12693
                     /gene="aroC"
                     /locus_tag="PLF77_07020"
     CDS             <11951..12693
                     /gene="aroC"
                     /locus_tag="PLF77_07020"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPC08640.1"
                     /translation="QKYGIRDWRGGGRASGRETAARVAAGAIAAKILDRVGIDVIAYT
                     KAIGSIAIDRGRMAAEKKLRTAVQENALFCPDLQAAARMERKITAVRKKGDSLGGIVE
                     IIVRGCPGGLGEPVFDKMDADLAKALMSIGSVKAVEVGDGFSMAGLQGSQTNDEIMPG
                     GFKTNHAGGILAGITNGEHIVLRAYCKPIPSIAKPQRTIDSKGKPRFIEIQGRHDICV
                     LPRIVPVCEAMVNIVVADHFLRQRAIQS"
     gene            12690..13475
                     /locus_tag="PLF77_07025"
     CDS             12690..13475
                     /locus_tag="PLF77_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HPC08641.1"
                     /translation="MKKINVGIIGGTNGMGKWFADLLKKEGCRVYVCGRKTRLHINDL
                     AGLCDVVVVAVPISATPEVIGQVGPQLTKDQLLMDLTSLKKEPVTRMLKTSQAEVIGC
                     HPLFGPQLKDVSGQNVILCPARGKKWLGWLKGVFKRSKMAVRESTPEKHDRMMAVVQA
                     LNHLDTMVLGLAIARTGMAFADIDKFSTPIFRIKMEIIRKVFTESPELYLDIIRGNSQ
                     TIKMLNIYEKALKDIRAVIRTRGKKESTEFVTKVAAKVHGRKN"
     gene            13459..14226
                     /locus_tag="PLF77_07030"
     CDS             13459..14226
                     /locus_tag="PLF77_07030"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPC08642.1"
                     /translation="MEEKINLVAAMIAASKKTVIFTGAGISTESGIPDFRGPDGLWTK
                     VDPEDFTIDRYLRSGETRKKVWYYLVEGGLMTTSQPNRAHLAIAELERMNKLSSIITQ
                     NIDNLHQRAGNDPKKVHELHGNMQWLVCLDCAERYDLEMMRQKHPSPDHFPICEKCAG
                     LLKPGVVFFGEMLPPDTLRMAEQESEKCDLFMVIGSSLVVYPAAYMPLYAKQSGAKIV
                     IVNMGQTGQNDIADVFINAPAGDTLSRIVARLKEIMT"
     gene            14249..>14463
                     /locus_tag="PLF77_07035"
     CDS             14249..>14463
                     /locus_tag="PLF77_07035"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HPC08643.1"
                     /translation="MCLIVFAYNVHPSYRLVLAANRDEFYERPSSPAGFWKDHPRMLA
                     GRDLKSGGTWLGITKDGRFAAVTNYRDP"
BASE COUNT         3745 a         3235 c         3973 g         3210 t
ORIGIN      
        1 aaatcagaaa cccaggtcag aattacgacg gtgtctcccg cgaaggcagg acgcagatat
       61 tcgatatgat gcatgcgcac cacccaggtg gctccgacat ccagggtggc tttggtgcat
      121 ccccggatgt cggaatgaag cacggcggcc cattgcatcc attgcaaata ttcaacgttg
      181 ttgacatgac catttatatc cacggcgctt tccggaaccg tgagtttata ttggtagact
      241 tgcgtcatga tgtaatttcc tgtttgataa aggggaataa taattgacat caattcatgt
      301 tttatgaata tagtaaatat ggtgattttg caacaggctt ctgaacataa cggcattgct
      361 tgccgatgaa gaataaaaat ttcaattatt gaggaaagga agtgcacgat ggtaaaagaa
      421 aaagaaagta atgttgcagg gaatctcgaa tcttattaca ccaaaccgaa taatctggtc
      481 gatatgtttg aagacagtgc ggcgaaattt gcgtcccgca atttgttcgg cacaaaaaac
      541 aaagagacca atcaatacga gtgggtaacc tttgcgcagg ttgccgagcg ggtgaacaat
      601 ctgcgcggcg cgttgaaaaa aatcggtttt gaaaaaggcg aaaaggtcgg tgtcattgtc
      661 ggcaactcgg cggaatggtt tgtttgcgcc aacgccactc acggtctggg cggtgttttt
      721 gttcccatgt acgaaaagga gctgcagaag gtgtggcagt acattatcaa ggattccgcc
      781 attaaatatc tttttgtcag caatcaaaaa atctacgaga tcattaaaaa ttttcaaaat
      841 gatattccaa cattaaaagg cgtgtttatg ctgtatggtt cgggagaaaa ttccctggcg
      901 gaactggaaa aaatgggcaa ggcaaatccc gttttgtcgt acaagccgca ctggtcggaa
      961 acggcggtga ttatttatac gtccggtacc accggcgatc cgaaaggcgt tctgctgagt
     1021 cacggcaatc tgacgcacaa cgccaaggaa agtcattgga cattcgatgt gggggaggaa
     1081 gaggtcgggc tgagcattct tccctgggcg cacagtttcg gtctgacgac cgacctgcat
     1141 gttttcgtct atggcggctg gcagctcgga ctggctgaat ccgccgacaa acttctgttg
     1201 aatttcggcg agatcaagcc cacgcatatg aatgccgtac cgcgggtgtt caatattatt
     1261 tacgataaaa ttcagcagag cgtgtcagcg gatccggaaa agaagaaaat cttcgatgcg
     1321 gcggtagccg aggcgatcaa aaaccgggat ctgaaagaaa aaacggacga atttaaattc
     1381 tatgacgcgg tcgccttttc cacggttcgg aacattttcg gcggacggtt aaaacaggtc
     1441 gtcattggcg gagcattgat gaaaccggaa atcgcgatgt tcttcagcga tgtcggggtc
     1501 gttccctacg tcggctatgg tttgtcggaa acatcgcctg ttcttaccaa caatagtccc
     1561 aagcggggca ataaattcgg cacgatcggc aaaccgtaca aagatacgaa agttgttatc
     1621 gataagtccc gcgtcggaga agacagtccg gacggcgaga tcgtggtgta tggcgcgcag
     1681 gtcatgcagg gatatcacaa caagccggaa acaaccaaag aggtgatgat gcccgatacc
     1741 tggaacggat tccccggcgt caggaccggc gaccgaggct ggattgatga agacggttat
     1801 ctgaatatca ccggacgttt caaggatgaa tacaaactgg aaaacggcaa gtacgttcat
     1861 cccgaatcca ttgaaaacga gatcaaactt ctgcgttacg tttccaatgt tattgtttat
     1921 ggcgagggcc ggacgtttaa cgtggcgctg gttgttccgg attttgaggc ggttaaaacc
     1981 gattcgcaaa cgtcccggtg ggcgcagggc acaccggacg aaaccattgc caataaggag
     2041 tgcaccgatt ttatctccaa acagatcacc gaccatttgc gcaagtcttt cggcggatat
     2101 gaaattcctc agaagttcct ttatatcacg gaggactttt ccgtggataa cggcatgctg
     2161 acgcaaaccc tgaaactcaa acgccgcaat gtcatggaaa agtacggtga gagactccgc
     2221 gctctttatg acaacgtgta aatcgaaaaa catgatatca tgttcaccat gagaacatga
     2281 tcatacagct cggaatactc tggaggtttt atgaacaatc aaaacgacgt ggtcattgtc
     2341 agtgcggtgc gcacgccttt cagtaaattc ggcggcgcgc tcaaagaaat tcacagcacc
     2401 gatctgggtg tgattgtcat caacgaaagc ctcaagcgcg cggggatgac cggtgaggat
     2461 ttggacgagg tttattacgg catgtgcgat caggccgagg ctgccctcta cgacaacgtc
     2521 aatgcgcggc aagccattct gcgcgcgggc ctgcccagca cgctcgtatc gcttaccttg
     2581 gatcgggcct gttgttcgtc cctttgcgcg gtgcagttgg gccgccgcgc cattttatta
     2641 aatgaggcgg acgcctgcat ggctgtgggc gctgagaaca tgagcaatac acctcttttg
     2701 atcaacggcc atcgctgggg ttcggggatg aagccgcttc tggtacagga ttacctgaat
     2761 cccattacct accgcgaata cacgccgctg ggacgcgatg cgggcgagat tgccatcgaa
     2821 cacggtattt cgcgggagga acaggatgcc tgggcgctgg ggtcgcatca gaaatgggat
     2881 gcggcggaca aggcggggct tttctatgat gaactggtca gcgtggagtt gccggcaaaa
     2941 aaaggaacgc cggtaatttt cggtaaagac gaacttcccc gtcccgacac caccctggaa
     3001 gcattggcca ggttaaaaac catttacggc agccccacgg tgacggcggg caatgctccg
     3061 gggttggatg ccggagcgac ggcggtcatt atcatgcgtc gatcaaaagc ggaaaagctc
     3121 ggtatcagac cgctggcaac gattgtggct gtggccagcg gtgcgggcga gcccagacgg
     3181 atggcggaac ttccggcaaa agtcatcgct caggcgctca agcgcgcatc gctttgcatc
     3241 gacgacatga gcgtcatcga aattaatgaa gcctttgccg ccgttaccct ggtttcaaca
     3301 aaaatgctgg ccggtggtga tgaagccaag tggcgcgagt tgctgaagaa aaccaatccc
     3361 aacggcggag ccattgccat cggtcatccg gtgggtgcca gcggcggaag aattttgatg
     3421 accatgatgt atgaactcaa gcgccgtggc ggcggttacg gcatctgcgg catatgcggc
     3481 ggactggctc agggcgacgc ggtcgtgatt cgtgtagatt gaattcggca cgggttttga
     3541 ggaatgtgtt gtgtcgccgt ttgttgaggt tagacaatat tccgttcgtt aacatgtaga
     3601 aaaggaggct caacatgaga gatgaaaaag gcattgcgtc ctgggttgtt atcgtaatca
     3661 ttgtgttgct gattgccgcc ggttttctat ggtcaaaata tttcgccgcc ccgacggcaa
     3721 cggtgatgat gcaaggcgcc gatcaggcga agccggccat tgaaaaagcg catcaggctt
     3781 ccgacgcggc cgaccgggcc aacaagattg ccgaagaggc cgcgaaaann nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg ttatgaaaaa agcactatcg tttgtttgga
     3961 tcttgatgct gttggctttt gccacagtgg cgcaggctga agttaaggca ggttcttttt
     4021 cagtaacccc tttcatcgga ggctatgtct ttgaaggtaa tgaatatgat aataatgaag
     4081 acacctacgc tgccggtttg cgcgccggtt acaattttac aaaaaatatc ggtgtggaag
     4141 cattttttaa ttatgttccg accaataaca aagactggaa tgacgacaat gaaatggaac
     4201 tcttcggata cggtctggaa gggctttatc attttatgcc cgaaagccgc tttgttccct
     4261 tttttgctgt aggggtaggt ggtattcatt atgatgcgct tcccgaggat ccggataacc
     4321 gggataaatt tgctctcgat tacggagccg gcttcaaata ttttgttacg gacaatattg
     4381 cattgagagc cgatgtccgt catatcattc catttaacga gcgatacaac aacctgcttt
     4441 actcggtcgg cctcaccttc gcctttggcg gcgagaaaaa gattgtagaa acaaaggttg
     4501 aagagcccct gcctcctcct ccgccacctg ctccggttgc caagccggtt atcattgaga
     4561 agggaagaca gaccctggat gtcaaatttg attttgataa agcaaccatt caggacggtt
     4621 attataaaga catcgatgcg ctggttgaag tgatgaaaga ttatccggaa ttgaatgtgg
     4681 tcatcgaagg acatacggat aatgtgggaa aggctgaata taataagaag ctctctcaaa
     4741 aacgcgccga aactgttaaa aaatacatgg tcgaaaaagg cggtgtcgat gccgcccgga
     4801 tcgaggcaaa aggctttggc gatgagagac ccgtcgatac aaacgatacg gccgatgggc
     4861 gtgcgaaaaa ccgtcgcgtg gaagctgcgg ttgattatat catcgaaaag taaagaagga
     4921 ttataaatcg tttcaacata aaagagcccc ggggatcact tttcggggct cctttatatt
     4981 tcttcttcat gacaatgcag gcttgaaacc tgatcggaag gatggcctgc cgcagtaaga
     5041 cgcccctccc ttttttcatc acgacgatca tgcaataaaa aaaggcttga caaatagtgt
     5101 caggttcatt agaaagcgta caaacaaaga caggaatctc acgatggaca gaaaacagaa
     5161 tgtaaatatg aatgtttgcg gttactggtt ttatacctat tttatatcgg tctgcccgtc
     5221 gctgaggagg actcgttaag gttcagtaaa ccaaaacagc catgggcgga tcgaaagcgc
     5281 tcatggcttt ttttttagaa agccagggcg tgcagacccc tggcttttta atttcaaagg
     5341 aggcaatatg attatcgtca tgaaaagtca tgcgaccgaa aaacagattg aagatgtgat
     5401 tcactggatt gaatccgtcg gatacaaggc ccatccttcc atcggggtgg aaagggcgat
     5461 catcggagcg gtgggtgaca atcggggtaa ggatattctg aaatttgcgg aatccctgcc
     5521 tggtgtggaa aagaccatgc ccattttgaa accctacaaa ctggccagtc gggaatcgca
     5581 tgatggtaat acggaggtga cggtaggaga ggtgaccgtc ggtggcccgg aatttgtggt
     5641 catggccgga ccctgcgcga ttgaaagtga agaacagttg ctggagtcgg cctatgtcgt
     5701 caaaaaaggc ggtgcgcaga ttctgagagg cggtgcctat aagcctcgca cgtcgcctta
     5761 cagctttcag gggatggagg aagagggcct gaaggtcttg aacaaggtcg gcactcaggc
     5821 cggtttgccg acggtgacag aggtcgtcaa tccggcggat gtcgatctgg tggaatctta
     5881 cgccgatatg ctgcagatcg gcgcccgcaa tgtgcagaat ttcgccctgt taaaaaaagt
     5941 cggtcattcg cgaaaaccgg tgctgttgaa gcggggtatg atgaccacga ttgaagagtt
     6001 gctgatgtcg gcggaataca tactctccga aggcaatccc aatgtgattc tgtgtgaacg
     6061 cggtatccgg acgtttgaaa cggccacgag aaatacgctg gatatcagtg ccatacctgt
     6121 tttgaaagga ttaacccatc tgccggtcat catcgacccc agtcatgccg ccgggaactg
     6181 gaaatacgtc attcccctgt cgcgggcagc ggttgctgtg ggtgcggacg gcattatcgt
     6241 tgaagttcat ccggagcccg agaaagcagt ttccgacggc gctcagtcac ttaaaccgga
     6301 aaagttctat cagatgatgg acgaaatacg ggccattacc gcaatcatga aacactagaa
     6361 gaacatttta tagtgaaggg aagacatcat gagatcgata aaagttaacc tggacagaaa
     6421 atcatcctcc tcttacgaaa tccgtatcgg aaaagacata tcggatcgtc tgtcgctgct
     6481 gatcgccaaa aaccataagg cggcacgtta tgcggtcatt acggacaata ttgtcgggga
     6541 actttacggt aaaaaatttc tgacggcgct aaaagagatc ggcctcaacg cgtcgttgat
     6601 tgattttcct gcgggagagt cctcaaaaac catcaaaacg gttgttgaca ttgccgaaac
     6661 attagcggtc tccggcgctg accgggagac atgtctgatt gctctgggcg gcggcgttgt
     6721 cggagatatt gccggtttcg ttgcctcgat ctatatgcgt tgcgtaccgt atatccagat
     6781 tccgacaacg ctggttgcgc aggtggacag cagtataggc ggtaaaaccg gcgtggacct
     6841 tccttgcgga aaaaatctgg cggggacttt ttatcagccg caggcggtca tgacgagcct
     6901 gcattttctc aatacgctgc ctccaaaaga attcaacaac gggctggcgg aaattataaa
     6961 atacggggtg attgatgatg aaaaaatgtt cgccagcctg gaagacatca tgacggcggt
     7021 caaatccaag gaccccaaaa tactgctcgg tgtcgtcgaa aaatgttgtc agatcaaaaa
     7081 atcaattgtg gaaattgatg aaaaagaagg gggcgtgcgt cgtattttaa atttcggcca
     7141 tacgatcggt cattcgctgg aagccgtttc cgggtatgcg attacccatg gagaaggcgt
     7201 ggcgattggg atggtggcgg ccgcgcgact ttccgccagg atgggttatc tgaaaaacaa
     7261 cgaggccggc cgtatcgaaa ccctcattga tcaggcgggc cttcccacga gaataccgaa
     7321 aacgctgtcc ggcggaaaaa ttgtggcaca attgaagatg gataaaaaga gaaaaggagc
     7381 cgcgattcat tttgtgctgc tgaaaaaaat agggttgccc tttattcatg gcaatatcga
     7441 gcagaaaatg attgccgacg tgatcgagga tatgaaatca tgaaaccgca cctgctggaa
     7501 aacttgcgaa aaaaaataaa agacaaggat aaggagattg tccgactttt gaacgagcgg
     7561 gcgcagatat ccgttcagat cggcaaaatg aaggggcagg gggggatgca ggtgtacgat
     7621 ccggcccagg aggcgaaggt ccacggatat ttacgggacc tcaatgccgg accgcttgcg
     7681 tccggggcgg tgtcggcgat tttcagggaa attatatcgg cctcgcgtga tttgcagaag
     7741 ccgatgacga ttgcctttct gggacccgag gcgtcctttt cccatctggc gtcgcgcctg
     7801 catttcggtg aatcgagtct cttctttcca cagacgggaa tattccgcgt ttttgacgag
     7861 gtggaaaagg gtgccattga ctggggtgtc gtgcccgtgg aaaattcgct ggaaggatcg
     7921 gtcaacgtga ccctggacag gctgatgata acgccgatga aaatcagggc ggagatatat
     7981 ctgcgaatcc gtcagtgtct tatgtcgtcg gccaaaaaca tgaaagaggt caaaagaatt
     8041 tattcacatc cgcaggcgtt ggcccagtgc caggtgtggg ttagaacaaa tcttcccaac
     8101 tgcgtggtat gcgaaacgga aagcacggca gccgctgtac agatgatccg gggaaagaag
     8161 catgaagccg ctatcgggag ttcgcttgcc gcaaagatat acggcgtgaa tctactggcc
     8221 gaaggcatcg aggataatcc ctccaacatg acgcgttttc tcgtaatcgg tcagggtgcg
     8281 aacgatccga ccggcaatga taaaacatcc ctgatttttg ccacaccgca ttctccgggg
     8341 gcgctgcatg ccgcgctgtc cgcctttgcc cgtcggaaaa tcaatctggc gaagattgaa
     8401 tcgcatcccg tgaaggataa attatgggag tacagtttct ttgtggatat gatcggccat
     8461 gcagatgaca ggcgtgtgca gggttgtctt cgagagctgc agaacaaaac gtcttttatg
     8521 aagattctgg ggtcctatcc caaaagcgag gatgcgctat gatttgtatt cccattacct
     8581 ccgacaaccc caaagaggct ttgcatgcca tagaacgaag ctgccagctg gccgactttc
     8641 tggaactgag aatggatctg atcggcaata tcggccttgc cgaattgatt tcagccgttc
     8701 gtgccgagtc tgctgcagta aagattattg tcaccagccg gaaaaaagaa gaggcggctt
     8761 ttgtcggaag atggccgcgg cgtcgaaacg cctgcagcaa aacgaggtat aaaatagcgc
     8821 tgctgaagga ggccgtcgaa cggggtgcgg attatattga cattgagctg gctgaaggaa
     8881 cacaggctat ccggcaactg caagcgttgt gtgcggaaaa aggttcgcaa accaagatga
     8941 taatttccta tcatcatctg aaagacaccc cggatcttgc aacattgaaa aaaatatttc
     9001 atcggtgtgc cgcgtataag ccggcagttg tgaaaattgt gacaacggcg agatccgaag
     9061 aggataatct tatcatatta aatcttattg cctatgcgcg caggaaatct caaaaaatca
     9121 tttcgctgtg catgggcgag aaaggcggca tcagccgcgc ggtggcgcct ctgatgggta
     9181 actttctgag ctttgctgca ctggaccgaa ccgggcgctc ggcccccggc cagtttaccg
     9241 ttcacgagat gaaccggatt caaaaatggt ttaaaggcaa gaacagagcc cctcagtcct
     9301 cagcgtcatc tccgcatgcg tctttacccc gccattacgt tttactgggc aatccggtcg
     9361 agcagagcct gtctcccgtg atgcatgatg cagccctgaa aaaaacaggt cttgcggaac
     9421 aatatgtcgc tttttgcgtt caggatatcg gcggtgcaat ggacgggttg agagcgatga
     9481 atattcacgg cgccagtgtg accattcctt tcaaggttgc cgtgatggaa tatttggatg
     9541 atattgatgc ggatgcgttg gagatcggag cggtgaatac gattgtcaat gaaaacggcc
     9601 gtctgatcgg acataacacg gattgggccg gattgatttt gacgcttaaa aaggcaatga
     9661 cgatcagaca taaaaccatt gtaatcgtcg gcgcaggcgg aaccgctcgg gcggcggctt
     9721 acggcatcct gaaagagggt ggatgtccgg ttattgtgaa tcgtaccccc aaaaaaggga
     9781 aaatgcttgc cgaaaaaatg cagtgtgctt tttatccgct ttccgagatc ggccgcatca
     9841 ggggggatgt actgatcaac acaacgcccg tgggaatgta tccccgggga aatcaatcgc
     9901 cggtcagcgc cgccgtattg tcggattacg actgcgtcat ggatgtgatc tataatccga
     9961 ttaaaacaaa attgctgagg gatgccgaaa agcagggttg ccgcattgta acgggtgtgg
    10021 aaatgtttgt taatcaaggc gctgagcaat tccggttatg gaccggcaag gaaccgccgc
    10081 atgcggttat gaaaaaaatc attctggaaa ggctgagcaa aggtggacga taatcctatc
    10141 cgttgcccgg acccggctgt gacggtccgc gttccgggat caaaaagctt gagccagcgg
    10201 gcgctggttg cggcaacgct ggcgcagggg gattccatga tcagcaatct gcttgtttct
    10261 gaggataccc tttacatgat cggtgcgttg cgggaactgg gcgcaaaaat tgtcgacgcg
    10321 ccggatggtt ttcaggtgac cggcacggcc ggcaaaattg aaaacagcgg cagggacctg
    10381 tttctgggca acaacggcac tgccctgagg ttcctgacgg ccctggtctg tctcggaaaa
    10441 ggccaatatg ttctgaccgg cgaaaaaagn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnng gcgtgaccat cagcaaaccg gagcgatttg cctatcgtgt caatgccgga
    10621 gagccttatc ggggacgcag atatgttgtc gaaggagatg cgtcgagcgc gtcgtatttt
    10681 tttctggcgg cggccctgct caagacaaca atccgggttg agggaatcag ccgggaaagc
    10741 aggcagggag acatccggct gctggatgtt ctggaaaaac tgggctgccg gttccggtcg
    10801 accacaaacg gagtggatgt caccggggga acactgttga aaggcgatag ggtcttcgac
    10861 ttcggcgata tgccggacat ggttccgacc ctggcggtct tgtccgcctt tcgcgaggga
    10921 cagaccgtta tcaacaatgt cgcgcatctg agaatcaaag agagcaatcg cctggcggct
    10981 gtcgcaaccg agcttaggcg ggccggtatt gatgcccgcg aaattccgga cggccttgtc
    11041 ataaagggcg gacgcatgcg gccggcaaaa atcgaaacct ataacgatca tcgcatggcc
    11101 atgagctttg cgatagccgg tcttgttgtt cccgggattg acatccgcga taaaaagtgc
    11161 gttgataaat cattcccctc gttctgggag gagctgcgtc gtttatgaag gttgttttaa
    11221 tcggttacag ggcgaccggc aagtccaccg tcggcaagat gctggctgcc cggcttaatg
    11281 ttgatttccg ggataccgat gccatgatcg aagaaagcat ggatatgccg gttaaggaaa
    11341 tcgtcgctct tcacggatgg gagtatttcc ggtcaaaaga aagagacatc atcaaatcgc
    11401 ttgtcaagaa agaagattgt gttattgcaa ccggaggcgg cgtggttctc ttcagagaaa
    11461 atgttgatct attgaaacag aacgttgatc tgctgaaaca ggccagtgtc atggtctggc
    11521 tcaacgctcc gctgcgcgac attgtggaac ggttaaaaca ggatgcgcaa aacgaagcaa
    11581 cgcgtccgcc gcttacatcc ggcaacattg ttcaggagac gatcgacacc atgagaaaaa
    11641 gatttcccct gtatgagcga tccgcggatt atgttgtgga tacgctgagc aaaacccccg
    11701 agcagattac ggaagaaatc tatcagtatt tgcttaaatc gggaaatttg gctaaaataa
    11761 ccgtcattaa aaaataaaaa ggaaaaaaat gtccggcaat acgacaggca gcgtttttcg
    11821 cgtcacaacc tggggagaat cacacggcag nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn tgcaaaaata cggaatccgc gactggcgag gcggcggacg ggcttccggc
    12001 agagaaacag ccgcccgtgt tgccgccgga gccattgcag caaagatact tgatcgggtc
    12061 ggaatcgacg tcatcgccta taccaaggca atcggttcca tcgcgattga ccgcggcaga
    12121 atggccgcag aaaaaaagct gagaacggcc gtgcaggaaa acgctctttt ttgtccggat
    12181 ctacaggcgg cagccaggat ggaaagaaaa attacggccg tccgtaaaaa aggagattcc
    12241 ctgggcggga tagtggaaat tatcgtccgc ggttgtcccg gagggctggg cgaaccggtt
    12301 ttcgacaaaa tggatgccga tctggccaag gccctgatga gcatcggatc ggtgaaagcg
    12361 gtcgaagtgg gtgacggatt ctccatggcc ggtttgcagg gctctcaaac caacgatgaa
    12421 ataatgcccg gaggatttaa aaccaatcat gcgggaggaa ttcttgcggg catcacgaac
    12481 ggagaacata tcgtcctgcg ggcttattgt aaacctattc cgtccattgc gaaaccgcag
    12541 cgcaccattg acagcaaagg caaaccccgg ttcattgaaa ttcagggaag gcacgatatc
    12601 tgcgttcttc cccgtattgt gccggtgtgc gaagccatgg tgaatattgt cgtggccgat
    12661 cacttcctca gacagagagc gatccaatca tgaaaaaaat taacgtgggc attatcggcg
    12721 gcaccaacgg catgggaaag tggtttgccg acctgctgaa aaaagaaggc tgccgggttt
    12781 atgtctgcgg cagaaaaacc cggttgcaca taaacgattt agccgggctc tgcgatgtgg
    12841 ttgttgtcgc tgtgcccatt tccgccacgc ctgaagtcat cggacaggtc ggcccgcaac
    12901 tgacaaagga tcagcttctg atggatttga catcgctgaa aaaagaaccg gtgacgcgga
    12961 tgctgaaaac gtcgcaggcg gaagtcatcg gctgtcatcc gcttttcggt ccgcagttaa
    13021 aagacgtatc cggacaaaac gtaatcctgt gtccggcgcg cggaaaaaaa tggctcggct
    13081 ggctgaaagg cgtttttaaa agaagcaaga tggccgtccg ggaatcaacg cccgaaaaac
    13141 atgaccgcat gatggccgtt gttcaggcgc tcaaccatct cgataccatg gtattggggc
    13201 tggctattgc caggacggga atggcttttg cggacatcga caaattttcc acgccgattt
    13261 ttcgcatcaa aatggaaatt atccgaaaag tatttacgga aagtcccgaa ctctatctgg
    13321 atattatccg gggaaattct caaaccatta aaatgctgaa catctatgaa aaagcattga
    13381 aagatattcg tgcggttatc cggacgcgcg gtaaaaaaga atcaacagaa tttgtaacaa
    13441 aggtggcagc aaaagttcat ggaagaaaaa attaatctgg tcgcggcaat gatcgcggca
    13501 tcgaaaaaaa ccgtcatttt taccggagcg ggcatcagca cggaatcggg tattccggat
    13561 ttcagagggc ccgacggtct ctggacgaag gttgatcccg aagatttcac gatcgaccgg
    13621 tacttgcgtt ccggcgagac gcgcaaaaag gtctggtact acctggtgga aggcggattg
    13681 atgaccacgt cccaacccaa cagggcgcat ctggcgattg ccgaattgga aaggatgaac
    13741 aagttaagtt caatcattac gcagaatatt gacaatctgc accagcgggc ggggaatgac
    13801 ccgaaaaagg ttcatgaact gcacggcaat atgcagtggc tggtctgcct ggactgcgct
    13861 gaacgctacg atctggaaat gatgaggcag aagcatccct caccggatca ttttccgatc
    13921 tgcgaaaaat gtgcggggtt gctcaaaccg ggagttgttt ttttcggtga aatgctgccc
    13981 ccggatacgt tgaggatggc cgagcaggaa tccgaaaaat gcgatttgtt catggtgatc
    14041 ggctcttccc tggtggttta tccggcggcg tacatgccgc tgtatgccaa acaatccggg
    14101 gcgaagatcg tcattgtgaa tatgggacag acgggacaga acgatatcgc cgacgtattc
    14161 atcaatgcac cggcgggaga tacgctgagc agaatcgtcg cccgattaaa agaaatcatg
    14221 acatgacttc cggatggtat gaaaccccat gtgtctgatc gtttttgcct ataatgtgca
    14281 tccgtcatac aggctggttc tggccgccaa ccgcgatgaa ttttatgaaa ggccgtcatc
    14341 gccggcgggc ttctggaaag atcatccgcg gatgctggcc ggccgcgatc tgaaaagcgg
    14401 cggaacgtgg ctgggcataa cgaaagacgg gcgattcgct gccgtcacga attatcgcga
    14461 ccc
//
LOCUS       DAOOLB010000047        14456 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4496_length_14456_cov_42.307895, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000047 DAOOLB010000000
VERSION     DAOOLB010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14456
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4496_length_14456_cov_42.307895"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..660)
                     /gene="thiE"
                     /locus_tag="PLF77_07040"
     CDS             complement(37..660)
                     /gene="thiE"
                     /locus_tag="PLF77_07040"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HPC08644.1"
                     /translation="MRGLYLVTDRRLCKDKSVEEIVLQAVKGGVSYVQLREKEMSTHD
                     FVEEALRIKKSLTPHKVPLIINDRVDVALACGADGVHIGQEDMPYDLARRLLGPKAII
                     GLSVETWADFIASQALDVSYIGVSPVFATPTKTDTKGVWGLEGLSRIKAFSRHPLVAI
                     GGIHEANIREVIEAGAPCIAVVSAICSSPDPETAAGRLARLINAALK"
     gene            complement(663..1460)
                     /gene="thiM"
                     /locus_tag="PLF77_07045"
     CDS             complement(663..1460)
                     /gene="thiM"
                     /locus_tag="PLF77_07045"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HPC08645.1"
                     /translation="MTAKEIYRSIELIRSRAPVIHNITNYVVMNNTANALLAIGASPV
                     MAHAQEEVEDMVDIASALVINIGTLSESWIRSMFKAARQARKKGIPVILDPVGAGATN
                     YRTNTARALMAQEPPSIIRGNASEIMALYDAQSKTKGVDSASSSDQAIDTAQKLSEMH
                     ECVVCVSGATDYIICPQTILKIKNGNPMMSRVTGLGCTASALCGAFAAVGESTFASTA
                     QAMALMGVAGETAAEVAAGPGSLQVHFLDALYRISEDDIARRLKVED"
     regulatory      complement(1641..1742)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1793..2803)
                     /gene="cydB"
                     /locus_tag="PLF77_07050"
     CDS             complement(1793..2803)
                     /gene="cydB"
                     /locus_tag="PLF77_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPC08646.1"
                     /translation="MDNIQCYQELWFVLILVLLMGYSLLDGFDLGIGALLPFLGKTKE
                     EKDILINSIGPVWDGNEVWLITGGGALFAAFPHAYATAFSGFYLAMMLVLFALIFRAV
                     SMEFRAHDEKRSLLWEKAFIAGSALAALLFGVALGNVVYGVPLDDQMEFTGNFFTLLR
                     PVPLMFGVTGFAAVLLQGAAYAVMKTEGELRERSFKAVSFLTVINLITAMIYFGTIAF
                     TFDDIVTNWLFYLAFLCTVLWLSSIFFSVRHKNDSAPFWESSFAFIGLWLVVAAVHFP
                     NLIRASNDQSMNLTIYNSSTSLLTLKVMSVIALIGMPIVIAYTIFVYRIFKGKTKSSS
                     HY"
     gene            complement(2848..4155)
                     /locus_tag="PLF77_07055"
     CDS             complement(2848..4155)
                     /locus_tag="PLF77_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPC08647.1"
                     /translation="MSAVILARLQFALTICFHFLFPAMTLGTTLIILISETLYLVKKD
                     ETYKKITDFLVRLLALIFVVGAATGIVMEFSFGTNWSGYSRAVGDIFGPILAAEGVLA
                     FFLESVFLGVLLFARNRVSPKVYWLSALLVFIGGHLSAFWIVVANSWMQTPAGYTINE
                     AGKIILGNFGDAVFNPSTVVRYVHTVIASWITSSVMVAGIAGYYVRKGLHGQTARTML
                     KIGIILFALTPLLQLGAGHAHAVNVIDLQPEKAAAMEGHFETAQGAKIYALGYVDEKN
                     RKTYGIYIPKGLSFLYNFDWNSEIKGLNDFPKEDWPPVNFVFQVYHIMVGAGVVMIGL
                     GLLGAYLLWKGRLYDNKWYLFMLPLLIPLPHIAHETGWIAAEVGRQPWIIYRLMKTSD
                     AASVVVSAGEITFSLIMFSLIYLLLGVMFIKLAITVVRNGPAA"
     gene            4324..4716
                     /locus_tag="PLF77_07060"
     CDS             4324..4716
                     /locus_tag="PLF77_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08648.1"
                     /translation="MKRKIVISAFALSFLFLVSMICFSTAEVCYADAPKDVKIEYDSG
                     AQTLTVTITHKSAFPGFHHIKNVDVKKNAVSVSQNAYSSQPKEVPFSYVYKVAAAPGD
                     KLDVTVVCNMSGSKTATLTVPAAGNAGK"
     gene            4703..6040
                     /locus_tag="PLF77_07065"
     CDS             4703..6040
                     /locus_tag="PLF77_07065"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPC08649.1"
                     /translation="MPENNFDIIISGGTLLTMSAGMDIIEDCFVGIHNGIITDIRSLK
                     KTRSTSIRAHETINADGCIVMPGLVNTHTHLPMVCFRGLADDLPLMEWLTGHIWPAEQ
                     RFVSKKMVYDGATLAMAEMILSGTTTFCDGYFFEDSLAEAVHVAGMRAVVAQGFADFV
                     MPDSAAVKKMMAAANRFVKKWYPHAPLLTPAFFCHSPYTCSPATLSAVKQSAREHGIL
                     YLVHLLENKDEVHTIRQRYGRTPVQHLLNLGVLDEQTVAVHCNWLTSEDMKIFADLGV
                     KVSHNPESSMKLASGVAPVPDMLKEGISVGIGTDGCASNNDLDMFREMDTTAKIHKVT
                     SLDPTVMNAESVCRMATIGGAHVLGLDKRIGSIETGKQADIILVDMNQPHLTPVYHYY
                     SQLVYAARGADVKTSIVNGKVIMKDRRLLTIDVPTAMKNVRTVAGGIIRKDSH"
     gene            6122..7045
                     /locus_tag="PLF77_07070"
     CDS             6122..7045
                     /locus_tag="PLF77_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HPC08650.1"
                     /translation="MNIVISGSMAYDRIMDFPGYFSDHILPDKIHMLNVCFQVNGMKE
                     KFGGTAGNIAYALRMLGEHPIICATIGHDYQKYFKWLQQNGISTESIKVIDEEFTAGA
                     YITTDRSDNQITGFNPGAMKYQCKIDFDKFDSRETIVIVSPGNFNDMVEYPRACKAKG
                     IDYILDPGQSLPMWDKNDLIQAIEGCRILIVNDYELSLIMSKTGLNKNALLKMAGTVI
                     TTLGELGSQVSTLTSDIAIPPVKLKKVVDPTGAGDAYRGGLLSGLVQGKDIVTSAKMG
                     SVSASYAVETYGTQEYSYTSEEYHQRLNDLL"
     gene            complement(7166..8542)
                     /locus_tag="PLF77_07075"
     CDS             complement(7166..8542)
                     /locus_tag="PLF77_07075"
                     /inference="COORDINATES: protein motif:HMM:NF010635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HPC08651.1"
                     /translation="MKINEAECLEKWSSMAASSSLVDSSLYAKYDVKRGLRDIKGKGV
                     LAGLTQIGEVQSHTQIGDDESELGPGCLIYRGIDITEIVSGFVADKRSGFEESCYLLL
                     FGELPDKEELERFQKLLISYRSLPDDFIHDSMLKLSSRDIMNAISQSILSMYVLDDRA
                     EDIEIPNVLRQCIKLIAVFPLLLVYCYQAYAYRYGNQSLVIHNPGRQLSTAANFLHML
                     RHDSKFTALEQKLLDLALVLHAEHGGGNNSSFTVHVVTSSGTDTYSAIAAAMGSLKGP
                     RHGGANIKVVRMFEDLKNNISNWNDDRQIEDYLIKLLNKQAFDRAGLIYGIGHAVYSV
                     SDPRTLILREYVGKLAQEKGLSEEMKLYERVESIAPEIIGHERKMYKGVSANVDFYSG
                     FLYRLLDIPPELYTPLFAMARIAGWSAHRIEELSNGGKIIRPAYKSVTPRRHYVPMKD
                     RNSNEQNR"
     gene            complement(8853..9446)
                     /locus_tag="PLF77_07080"
     CDS             complement(8853..9446)
                     /locus_tag="PLF77_07080"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: protein motif:HMM:NF014690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HPC08652.1"
                     /translation="MGKTFSIFLLAYLAGSVNFAIIFFKLTGRKDPRLGFSGNAGTTN
                     VYRQAGMVWAAVIFLLDIGRAMLVAVLAMYYLEPAWLPWAGYFLVLGNSYPCFHEFRG
                     GKGVANYLGFTILVSPWAAMLSCAVWLLVYGLVRMPFIASFFMVFTLACGQAYHSHWN
                     WGAIAGSLMTWILILYNHRINIMAHRTREKDVPHQNK"
     gene            complement(9549..10412)
                     /locus_tag="PLF77_07085"
     CDS             complement(9549..10412)
                     /locus_tag="PLF77_07085"
                     /inference="COORDINATES: protein motif:HMM:NF026115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HPC08653.1"
                     /translation="MENISGLNERQRYPFDLEIYPYLRDHHLEKRAILPAVESLIILA
                     GACRAVSLQRGIKCIRKAAFSRFLYLAPDNRRQPAVIDIIKTEGGNMVASLLTSVQSK
                     TGTISRHVEHARAECVISGFEETGPPSFRSVDKLKGSCISIPSATVYRDLVPFGIAYQ
                     NIIGDLSVSSEGALGYVSGGNFEADDELLGSPFPLDAVMHAACVWGQRFAGKVCFPVG
                     FEKRLIYQKTEKGQEYLGRVVPVSITRESLIFDVWIYKDDVLYESVSGIIMKDVTNGR
                     MNPPDWIKVNS"
     gene            complement(10391..10870)
                     /locus_tag="PLF77_07090"
     CDS             complement(10391..10870)
                     /locus_tag="PLF77_07090"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPC08654.1"
                     /translation="MKPKPFKPEIYHNDERYVRDLTTGLVWHRSVYRVLYIDTDRSQV
                     VYHSNYLRYFEFGRTSLMRDLAYPYKEIEDGGYVYPIFDLGISFHQPLYYDDVMYIHT
                     RPVNLERVRLQFDYLITHQEKGNIICAGFTKHCALNLSGTPVAVDAKTVHLWKTFPV"
     gene            complement(10872..12137)
                     /locus_tag="PLF77_07095"
     CDS             complement(10872..12137)
                     /locus_tag="PLF77_07095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPC08655.1"
                     /translation="MRAPKNRRVFVVGYGAATPLGSTFEKTWKRAVKGEAGFRKVTRC
                     KVESACDVVGEIPDWYPMELDFTDAKEVYNWNAAFVILTMAVCKEALENAGIVVDAQI
                     APRMACLIGSALNGSDAFRIAVHDLDHRGPLRVSPYLLPNLCANLPSGKAGMLLKFTG
                     PIFSPQGACASGNHAIGIGARMIRDGDCDFVLAGGADTPILPELIHGFANMNATIKVN
                     PKDRAYDNPALASRPFSADRKGFVLSEGAGVVVLAADEAIKTYGLKPKAEVLGIGWTS
                     DAHHYTSPNMPTIIRAIRETIDDAELEPTDIQYINAHGTSTPKGDTTEIKCLREVFGK
                     KIEKIPVSSNKSQLGHTLGATAAIEAALTIEGMKKGIILPTINHVPDPEFSDIDVVPN
                     EARKNKCDIALSNAFGFGGTNCCVIFRGV"
     gene            complement(12229..13479)
                     /locus_tag="PLF77_07100"
     CDS             complement(12229..13479)
                     /locus_tag="PLF77_07100"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPC08656.1"
                     /translation="MGLKKTVIVGYDAVSSLGAQLDAQWRQAVAGKSGIGRLTRFPLS
                     ENFPVRVAGEVEEIDPGPYPFLQPREMAHWTSPIFKYALLVVHRALQNGGIEITRDIA
                     PRVAITFSSAVGGLDAVLQADRLMVKDGKLPHPFTNPNSCINMVGGKVSILTGATGPI
                     CSTITACATGITSMIIGEMLLQRNMADVVIAGAVDFPLVEPIVAGFATMNGAYRPKEG
                     QPEEPPEKTSRPFSANRRGFVVSEGAGCIILATGEFAKVHGLPSGMEMAGWAMTSDAS
                     HFVSPNLATVRKCIEETIANAGLKPEDIDAVNAHATSTKIGDKVEADALKNIFGGHVP
                     PVSANKSQMGHAMGASSAIEAILAMEGMKKEIVLPTINYRPDQDIAIDCVAEGARNLK
                     QEFVLKNAFGFGGCNSCIILHRTE"
     gene            complement(13569..>14456)
                     /locus_tag="PLF77_07105"
     CDS             complement(13569..>14456)
                     /locus_tag="PLF77_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08657.1"
                     /translation="RCKGCFFFSSGEHQAAVEEMDIGKWEAFIDREKERGVNLAILIG
                     GEPTLCLDRVEAFYKRLPTYCATNGLIKVPRDRFPDMMVGISLWGSAEDEKILRGRDT
                     FAVSSKNYEGDAYTYYLYTITPRQVGKIEPVIKRIADVGLKVHLQLLSNDEGVDGFSW
                     QEGELKDLCHEMDEMLDTYPQTVISCKYYHEIITTGKMMGRRFGWNECPSVTEALDNR
                     ANRPRRLIHFARWASDLKTVHRCCTSATRDCSTCKDGAAHMSWVMVNKREHLKSTKDL
                     QNWIEVYEMFAKLYHFIPW"
BASE COUNT         3751 a         3844 c         3370 g         3491 t
ORIGIN      
        1 tccgtcggag gcgcatccgt ctttggataa ctcaatttat ttcaaggcgg cattgattaa
       61 gcgcgccaac cgaccggcag cggtctccgg atcgggcgag gaacatatcg cagaaacaac
      121 cgcgatgcac ggtgctccgg cttcaatgac ctccctgata ttggcttcat gaataccgcc
      181 gatcgccacc agcggatgac gactgaatgc cttgatgcgg gaaagacctt ccaaacccca
      241 tacacctttc gtatccgttt tggtcggtgt ggcaaaaacc ggactgacgc caatataact
      301 gacatcaagc gcctgactgg caataaaatc cgcccacgtt tctacggaaa gaccgatgat
      361 cgctttgggt cccagcagtc tgcgcgccag gtcataaggc atatcctcct gtccgatatg
      421 aacgccgtcg gcgccgcagg ccaaagcaac atccacacgg tcattgatga tcaggggaac
      481 tttatggggc gttaaactct tcttaatacg cagggcttct tcaacaaaat catgggtcga
      541 catttctttt tcgcgaagct gaacatatga gactccgcct ttgaccgcct gaagaacgat
      601 ttcctctacc gatttgtctt tgcacaaacg gcggtcggta accagatata atccccgcat
      661 gtctaatcct caacctttaa acgtctcgcg atatcatctt ccgatatacg gtaaagagca
      721 tccaaaaaat gaacctgaag gcttcccggt ccggcggcaa cttctgccgc cgtttccccg
      781 gcaaccccca tgagcgccat cgcctgagcg gtggaagcaa aagtcgattc tcccaccgcg
      841 gcaaaagctc cgcataacgc cgatgccgta cagcccaatc ctgtgaccct ggacatcatg
      901 gggttaccat tttttatttt taagattgtt tgcggacaga taatgtaatc tgttgctccg
      961 ctcacgcata cgacacactc atgcatttcg cttaattttt gcgctgtatc tatggcctga
     1021 tcggaagacg aggcgctgtc cacccctttt gttttggatt gcgcatcgta aagagccatg
     1081 atttccgacg cattacccct gatgatcgaa ggcggttctt gcgccattaa tgctcgcgcc
     1141 gtattcgtac ggtagttggt cgctccggcg ccaaccggat ccagaattac gggaattcct
     1201 ttttttcggg cctgacgcgc ggctttgaac atcgagcgga tccagctttc acttaaggtt
     1261 ccgatgttga tcaccagagc cgacgcgatg tccaccatgt cttcaacttc ctcttgcgca
     1321 tgcgccatga ccggagatgc gccgattgcc aaaagcgcgt tggccgtatt gttcatgacc
     1381 acgtaattgg ttatgttatg gatcaccggc gctctggaac gaatcagctc tatcgatctg
     1441 taaatttctt tcgccgtcat tctcatgatc ttccagctcc ttgatggttt gcaaaaccca
     1501 aagcaatatt taaagggata cttccccaca aaaacaagtc gccccgaggg gatcattcga
     1561 tcagcagtct tcggtgcact ccgtttccgg agtttggcct tcatttaaaa cccttcccga
     1621 cgggccatac cgggaagggt ttacatgcaa caaacattct cccttcgctg gcattatcca
     1681 gatcaggttc aacgggtatc atctcagatc cgttatcttc cggaccaccc ctatgcaaaa
     1741 tatctgacca atgtgattga taggcgtcaa tcacattcat tgtcgctgcc tttcaataat
     1801 gacttgaaga tttcgttttg cctttgaaga tacgataaac aaaaatggta taggcaatta
     1861 caatcggcat cccgatcaga gcaataacgc tcatgacttt gagggttaac aaactggtgg
     1921 aactgttata aattgtcaag ttcatacttt gatcattgct ggccctaatc agattgggaa
     1981 aatgaacggc ggccacgacc agccacaggc cgataaaggc gaatgacgat tcccaaaacg
     2041 gagcagagtc gtttttatgc ctgacggaaa agaaaatcga tgacagccaa agcacggtgc
     2101 acagaaaggc cagatagaat aaccaattcg taacgatgtc atcgaaggta aaggcaatcg
     2161 ttccaaaata gatcatggct gttatgagat ttattaccgt tagaaaactc accgctttaa
     2221 aggaccgttc tcgcaactca ccttccgttt tcatcactgc gtatgctgct ccctgcaaca
     2281 aaacagcggc aaacccggtt accccgaaca tcagcggcac cggacgcagt agtgtaaaga
     2341 agtttcctgt aaattccatt tgatcatcca gcggcacccc gtaaacgacg ttgcccagtg
     2401 cgacgccgaa caacaaagcc gccagcgcgc tgccggcaat gaacgccttt tcccaaagga
     2461 gggatctttt ttcgtcatgt gcacgaaatt ccatggaaac agcccggaaa atcagagcga
     2521 acaacaccag catcatcgcc agataaaaac cggaaaaagc cgtggcatag gcatgcggga
     2581 aagcggcaaa taaagcgccg ccgccggtaa tcagccagac ttcattgccg tcccaaaccg
     2641 gaccgatgga attgattaag atatccttct cttctttggt cttgcccaaa aaaggaagca
     2701 gcgctccgat gcccagatca aatccgtcca gaagagaata ccccatgaga agcactaaga
     2761 tcagaacaaa ccagagctcc tgatagcatt gaatattgtc catggatgtt gcctccagaa
     2821 taatttccag tttttagaat tttttcatta agcggccggt ccattccgaa cgacggtgat
     2881 ggccagtttg ataaacatca ctcccaagag gagataaata agagaaaaca tgatcaggct
     2941 gaacgttatt tctcccgcgg aaacaaccac cgatgcggca tccgaggtct tcataagcct
     3001 gtagataatc caaggctggc gtcctacttc cgctgcgatc cagccggtct catgggcaat
     3061 atgcggcagc ggtatcagga ggggcaacat aaaaagatac catttattgt cataaagtcg
     3121 acccttccat aacagatacg cccccagcaa tcccaggccg atcatgacga ctccggcacc
     3181 caccatgata tgatacacct gaaacacaaa gtttaccggc ggccagtctt ctttcggaaa
     3241 gtcattgagg ccttttattt ccgagttcca gtcaaaattg taaagaaaac tcagcccctt
     3301 gggaatgtag atgccatacg tcttgcggtt cttctcatca acataaccca gggcatatat
     3361 cttggcgcct tgcgctgttt caaaatgccc ttccatcgcg gctgcttttt ccggctgcag
     3421 atcgatgaca ttcaccgcgt gggcatgccc cgcccccaat tgcagaagcg gtgtcagagc
     3481 aaaaaggatg atcccgattt taagcatggt tctggccgtc tgaccgtgga gaccttttct
     3541 cacataatac ccggcaatac ccgccaccat caccgaactg gtgatccatg aagcgatgac
     3601 cgtatggaca taccggacga ccgttgacgg attaaaaacc gcgtcgccaa aatttcctaa
     3661 aatgatttta cccgcttcat taatggtata acctgccggt gtctgcatcc aggaattggc
     3721 caccacaatc cagaaggcgg aaagatggcc gccgataaaa accagcagag cggataacca
     3781 gtatactttc ggggacactc tgttgcgggc aaaaagcagt acgccaagga aaaccgattc
     3841 cagaaaaaac gccaatacac cctctgcagc caggatcggc ccgaaaatgt ctccgaccgc
     3901 gcgggaatat cccgaccagt tggtgccgaa tgaaaattcc atgacaatac ctgttgcagc
     3961 cccgacaaca aaaatcaacg ccagaagcct gaccagaaaa tccgttattt ttttatacgt
     4021 ctcgtccttc ttcaccagat aaagcgtttc ggatatcagg ataatcagag ttgtgcccag
     4081 cgtcatggcg gggaaaagaa aatgaaaaca tatcgtcagg gcaaactgta accgggcaag
     4141 gatgactgcg ctcataagca cctcatttcg gcagttgaag ttggcttgta ctgttccatt
     4201 tttttcaaca ctagccaatt atccgcaact tttcaactca taatttttcg ctgttatttg
     4261 cgcagtgaat atattatcat ttagcatgaa cagcacagaa cattaaagat caggaggtct
     4321 gccatgaaaa gaaaaattgt catctctgct tttgctttga gttttttgtt tcttgtatca
     4381 atgatttgct tttcaaccgc tgaggtctgt tacgccgacg cgcccaagga tgtaaaaatc
     4441 gaatacgatt ccggagcgca aaccctgacg gtcacgatca cgcataaatc cgcgttcccc
     4501 ggatttcatc acattaaaaa tgtcgacgtg aaaaagaatg cggtttccgt cagtcaaaac
     4561 gcctactcat cgcaacccaa agaggtgccg ttcagctacg tatataaggt ggctgccgct
     4621 cccggcgata agctggatgt aacggttgtt tgcaacatgt ccggaagcaa gacggcaaca
     4681 ttgacggtgc ccgcagcagg aaatgccgga aaataatttc gacatcatca tttccggcgg
     4741 cacactgtta accatgtccg ccggcatgga catcatcgag gattgttttg tcggcattca
     4801 caacggcatc atcaccgata tccgttccct gaaaaagacc cggtctacct ccatccgggc
     4861 gcacgaaacc atcaacgcgg acggctgtat cgtgatgccc ggcctggtga atacacacac
     4921 acatctgcca atggtctgtt tccggggact ggccgacgat ctgcctctca tggaatggct
     4981 gaccggtcat atctggccgg cggaacagcg tttcgtcagc aaaaaaatgg tgtatgacgg
     5041 cgcaacgctg gccatggccg aaatgattct gtcgggaacg acaaccttct gcgacggata
     5101 tttctttgaa gacagcctcg ccgaggccgt ccatgtcgcc gggatgcgcg ccgtcgtcgc
     5161 ccagggcttt gccgattttg tcatgccgga tagcgcggcc gtaaaaaaga tgatggcggc
     5221 agccaaccgt tttgtaaaga aatggtatcc ccatgcgccg ctcttgactc cagctttctt
     5281 ctgtcattcg ccctatacct gttctcccgc aacactctcc gcagtcaagc aatccgcccg
     5341 cgaacacggt attttatatt tggttcatct actggaaaac aaggacgaag tccacaccat
     5401 ccggcagcgc tacggccgga caccggtcca gcatctgctg aacctgggcg tgctggacga
     5461 acaaaccgta gccgttcact gcaactggct aacatcagaa gatatgaaaa ttttcgccga
     5521 tctgggagta aaggtttcac acaatccgga aagttccatg aaactcgcct ccggcgttgc
     5581 gccggttccc gacatgttga aggaaggaat atccgtcggc attggaacgg acggctgcgc
     5641 cagcaacaat gacctggata tgttccggga aatggacaca acggcaaaaa ttcataaagt
     5701 aacttctctc gacccgacgg tcatgaatgc tgaatccgtc tgccggatgg ccaccattgg
     5761 cggggcacac gttctgggac tggacaaacg aatcggttcc atcgaaacgg gaaagcaagc
     5821 ggacatcatt ttagtggata tgaaccagcc tcatctgacg cctgtctatc actattactc
     5881 tcaacttgtc tatgccgcac gcggcgcgga tgtcaaaacc agcattgtca acggcaaggt
     5941 gatcatgaaa gaccggcgtt tgctcacgat cgatgttccg accgccatga agaatgtgcg
     6001 gacggtagcc ggcggcatca tcagaaaaga ttcgcactga atattgtcca acaatgtcat
     6061 tgacacaaaa atcaatattt cctatatgtt ttcaaaataa tctgaaggac tgatttaaat
     6121 tatgaatatc gttatttccg gttcaatggc gtatgacaga atcatggatt ttcccggcta
     6181 tttttccgac catattctgc cggataaaat ccacatgctc aatgtctgct ttcaggtaaa
     6241 cggcatgaag gaaaaattcg gcggcacggc cggcaacatt gcttacgctc tgagaatgct
     6301 cggcgaacat cccatcatct gtgccaccat cggccacgat taccagaaat acttcaagtg
     6361 gcttcagcaa aacggcatct ccacggaaag catcaaagtc atcgatgagg agtttactgc
     6421 cggtgcctac attaccaccg accggtccga taatcagatt accgggttca atcccggcgc
     6481 aatgaaatac caatgtaaga tcgattttga caaattcgac tcccgggaaa caatcgtcat
     6541 cgtctcgccc ggcaatttca acgacatggt agagtatccg cgtgcctgca aagccaaagg
     6601 catcgattac attctggatc ccgggcagtc tctgccgatg tgggacaaaa acgatctgat
     6661 tcaggccata gaaggctgcc ggattctgat tgtcaacgat tacgaactca gcctcatcat
     6721 gagcaaaacc ggcctgaata aaaacgcgct gctgaaaatg gccggaaccg tcatcaccac
     6781 gctgggagaa ctgggttcgc aagtctccac gctgaccagc gacatcgcca tccctccggt
     6841 caaattaaaa aaagtggtcg atccgacagg cgccggagac gcctaccgtg gcggtctgtt
     6901 gagcggtctg gttcagggga aggacattgt gacgagcgca aaaatgggca gcgtttcggc
     6961 gtcctacgcc gtggaaacct acggcacgca ggaatacagc tacacgtccg aagaatatca
     7021 tcaaagacta aacgatttat tataaccgca gacaacactc ctgacattcc ggaggcgtgc
     7081 tcaataaaaa atcattctcc aatttttttt tgcggcgtca ttagaaaaaa tccgctgatg
     7141 atcaacggct gttttcacgg ccgggctatc tgttctgctc gttgctgttt cgatccttca
     7201 taggcacata gtgtcggcgc ggcgttacgc ttttataggc gggcctgatg atttttcctc
     7261 cgttggacag ctcttctatc cggtgcgcac tccagccggc aattctggcc atcgcgaaaa
     7321 gcggcgtgta caattcaggc ggaatatcca ggagacgata aagaaacccc gaataaaaat
     7381 cgacgttggc actaacgccc ttgtacattt ttcgctcatg tccgatgatt tcaggggcta
     7441 tactctcgac tctctcatag agtttcattt cttcggagag tcccttctcc tgcgcgagtt
     7501 ttccgacata ttcccttaaa atcaacgtgc gcggatcgga aaccgaataa acagcatgac
     7561 cgataccgta aatcagaccg gcccggtcaa aagcctgctt attgagaagt ttgatcagat
     7621 aatcttcgat ctggcggtca tcattccagt tggaaatatt atttttcagg tcctcaaaca
     7681 ttcgcacaac tttgatattg gccccgccgt gacgcggacc cttgagcgac cccatggctg
     7741 cggcaatggc ggaataggta tccgtaccgc tggacgtaac cacatgcacc gtgaaagagg
     7801 aattattacc gccgccgtgt tccgcgtgaa gcaccagtgc caaatccagc agcttttgct
     7861 ccagagccgt gaatttgctg tcatgcctga gcatatgcag gaaatttgcg gcggttgata
     7921 actgccgtcc gggattgtga atcaccaggc tttgattgcc gtaacgatag gcgtatgcct
     7981 gataacaata caccaggagc aacgggaaca ccgcaatcag cttaatgcat tgccggagga
     8041 cattcggaat ctcgatatcc tcggcccgat cgtccaaaac atacatggac aagatactct
     8101 gggaaatggc attcatgata tcgcggctgg aaagtttgag catggagtcg tggataaaat
     8161 catccggtaa agaacgataa gaaatcagca gtttttgaaa ccgttccagt tcctccttat
     8221 cgggcagttc accgaaaagc agcagataac aggattcctc aaagccactg cgtttgtccg
     8281 cgacaaaccc ggatacaatt tcggttatgt cgatccctcg gtaaataagg caccccggtc
     8341 cgagttcaga ttcatcatcg ccgatctgcg tatgagactg cacctcaccg atttgcgtca
     8401 gcccggccag cacccccttg cctttgatgt cgcgcaatcc tcttttaaca tcatatttgg
     8461 catacaagga agaatccacc aggctgctgg atgccgccat tgagctccat ttctcaaggc
     8521 attccgcttc atttattttc atatgtttct ccttccctgt tttgtgtatt tcgccgaata
     8581 ttcagacaaa ataaaatagt attctgcaga tcggtatttt acaatacctt ataattcaag
     8641 gacaaaagtg cttttttgac gggcaacatt gaatgaaaga atgtagcctc tgaagtgtgc
     8701 tttctaatat tgcgctgtgc ccaaatgaca tttttaatat agtcaatgac agcgggtacg
     8761 tcaattattt ttattttgaa cattgtcagc ggataaaaac ggttggttct taaagtataa
     8821 ctttaagtct taaatactat ttcatttcac cgttatttat tttgatgtgg cacatctttt
     8881 tctcgcgtgc gatgcgccat gatatttatt ctatgattgt acagaatgag tatccatgtc
     8941 attaaagaac ctgcgatcgc gccccaattc caatgggaat gataagcctg tccgcaggcg
     9001 agagtaaaaa ccataaaaaa agaagcaata aaaggcatcc ggactagtcc gtaaacaagc
     9061 agccagacag cgcaggaaag catcgctgcc cacggtgaaa caagaatcgt aaatcccaga
     9121 tagttggcca ccccttttcc gccgcgaaat tcatgaaaac acggatagct gttgccgagg
     9181 acaagaaaat aaccggccca ggggagccat gcgggttcca aataatacat ggctagcacc
     9241 gcgaccagca tcgcccggcc gatgtcgagc agaaaaataa cggccgccca caccatgcct
     9301 gcctgacggt aaacgtttgt ggttcccgca ttgccgctga acccgagacg gggatccttt
     9361 ctgcctgtca gtttaaaaaa aataatagca aaattaactg atccagccag atacgctaaa
     9421 agaaatattg aaaatgtttt acccataatg agactcgctg agaacaagta aaacgaagtt
     9481 tgaagcgcta gtcaacttat ctcaggcacg tcgtgccgcg ggatacccgt caattttacc
     9541 cttcgctctc aactattaac cttgatccaa tccggcgggt tcatccttcc attcgttaca
     9601 tctttcatta taatgccgct gacgctctca tagagaacgt cgtccttgta aatccagaca
     9661 tcaaaaatca gcgattccct ggttatgctg accggaacaa cacggccgag atattcctgc
     9721 cctttttccg ttttttgata aatgagtctt ttttcaaatc caaccgggaa gcaaactttg
     9781 ccggcaaagc gctgccccca gacgcaggca gcgtgcatga cagcatccag aggaaaaggg
     9841 gatccgagca attcatcgtc agcctcaaaa tttccacctg aaacgtatcc cagagcgcct
     9901 tcggaagaga ccgacaaatc gccgatgatg ttttgatagg caatgccgaa aggaaccaga
     9961 tcgcgataga cggtagcgga cggaatgctg atacagctgc ccttcagttt atccaccgag
    10021 cgaaaagaag gcggacctgt ttcttcaaaa cccgagatca cacattccgc tcttgcgtgt
    10081 tcgacatgac ggctgattgt gcctgttttt gactggacgg acgtcagaag cgacgcaacc
    10141 atgttgccgc cctccgtttt tattatatcg ataaccgccg gctggcgacg gttatccggc
    10201 gccaggtaaa gaaatcttga aaaagcggct ttccgaatgc attttatccc gcgctgcaaa
    10261 gaaacagccc ggcacgctcc ggccaaaata atcagcgatt ccactgcagg gaggatcgct
    10321 cttttttcca ggtgatgatc ccgcagatat ggatatatct cgagatcaaa cggataccgc
    10381 tggcgttcat ttaaaccgga aatgttttcc atagatgaac cgttttcgca tccacagcca
    10441 cgggcgtgcc tgacagattc aaggcgcaat gtttagtaaa accggcgcag atgatgtttc
    10501 ctttttcctg atgcgtaatc agatagtcga actgcagacg cacccgctcc agattgaccg
    10561 gccgtgtatg gatatacatg acgtcatcat aataaagagg ctgatgaaag ctgatgccaa
    10621 gatcgaaaat gggataaaca tagccgccgt cttcgatctc cttgtacgga taggcaagat
    10681 cgcgcatcag cgacgtgcgg ccgaattcaa aataacgcag atagttggaa tgataaacca
    10741 cctgcgagcg atccgtatcg atatacaaaa ccctgtacac tgaacgatgc cagacaaggc
    10801 cggttgtcag atcgcgcacg tagcgttcat cgttatggta tatttcaggc ttgaaaggtt
    10861 tgggtttcat cttagactcc tctgaaaatg acacagcagt ttgttccgcc aaatccgaat
    10921 gcgttggaaa gagcgatgtc acatttattt tttcgggcct cattgggtac gacatcaatg
    10981 tcggaaaatt ccggatcggg cacgtgattg atggtcggta aaataattcc ttttttcatt
    11041 ccttcaatcg tcagggccgc ttcaatggcc gcggttgctc ccagcgtatg ccccagttgc
    11101 gatttatttg acgatacggg aattttttcg atttttttgc cgaacacctc gcgcaggcat
    11161 tttatttctg tggtatcccc cttcggtgtc gaggttccgt gggcattgat atattgaata
    11221 tctgtcggct ccagttccgc atcgtcaatc gtttcccgga tggcacggat gattgtcggc
    11281 atgttggggc tggtgtaatg atgagcatcc gacgtccagc cgatgcccag aacttcggct
    11341 ttcggtttga ggccataggt tttgatcgcc tcatccgccg ccagaacaac aacgccggct
    11401 ccctccgata gaacaaatcc cttacgatca gcactgaatg gcctcgaagc cagtgcggga
    11461 ttatcatatg cccgatcttt aggattaact tttatggtgg cattcatatt agcaaaaccg
    11521 tgaatcagct ccggcagaat gggcgtatcg gcgccgccgg ccagaacaaa atcgcaatcg
    11581 ccgtcccgga tcattctcgc cccgatgccg atggcatggt tgccggaggc gcaggcgcct
    11641 tgcggcgaaa aaatcggtcc ggtaaatttc aacagcatgc ctgccttccc ggagggaaga
    11701 ttcgcgcaca aattgggaag cagatacggg ctgacccgca gtggtccgcg gtgatccaga
    11761 tcatgcacgg caatgcgaaa agcatccgag ccgttgagtg ccgaaccgat cagacaggcc
    11821 atccgcggag caatctgtgc gtcgacaacg ataccggcat tttccagtgc ctccttgcaa
    11881 acggccatcg tcaggatcac aaacgccgcg ttccagttat aaacttcttt ggcgtcggta
    11941 aaatcaagtt ccatcggata ccagtcgggg atttctccga ccacgtcgca ggcggattcc
    12001 actttacagc gggtaacctt gcggaaaccg gcctcgcctt tcaccgccct cttccacgtt
    12061 ttttcaaacg tgctgcccag cggtgtggct gcaccgtaac cgacgacaaa aactcttctg
    12121 tttttgggcg ctctcataat ttgccttttt tatgtcattt cgggtgtctt gagcaatctt
    12181 ttctttaaat tgccatatcg accagggatt gtctcgccat gacaatcatc attctgttct
    12241 gtgcaagatg atgcaggaat tgcatccgcc gaagccgaac gcatttttca agacaaattc
    12301 ctgtttaagg ttcctcgcac cttccgcgac gcagtctatt gcaatgtcct gatcaggacg
    12361 gtaattgatc gtcggcaaaa caatctcttt tttcatgcct tccatcgcga ggatggcttc
    12421 aatggcgctg gaggcgccca tcgcatggcc catttgagat ttattcgccg ataccggcgg
    12481 cacatggccg ccgaaaatat ttttcaaagc atcggcctcc actttatcgc cgatctttgt
    12541 tgacgtcgca tgcgcattaa ccgcatcgat gtcctccggc tttaatccgg cattggctat
    12601 tgtttcttcg atgcacttcc gcacagttgc caaattcggg gacacaaagt gactggcgtc
    12661 cgatgtcatg gcccatccgg ccatttccat gccggacggg agcccgtgga cttttgcaaa
    12721 ttcgcccgtt gccagaataa tgcaaccggc tccttcggaa acgacaaaac cgcgccggtt
    12781 tgcagaaaac ggtctgctgg ttttttccgg cggttcttca ggctgtccct ctttgggccg
    12841 gtatgcgccg ttcatcgtgg caaagcccgc aacaatcggc tccaccagcg gaaaatccac
    12901 cgcaccggca atcaccacat ccgccatatt tcgctgcaac agcatctcgc caatgatcat
    12961 cgatgtaatg ccggtagcac aggccgtgat ggttgaacaa atcggtcccg ttgcacccgt
    13021 gagaatggag accttgccgc cgaccatatt gatgcaggaa ttgggattgg taaacggatg
    13081 aggcagcttg ccgtccttca ccatcaggcg atcggcctgc aaaacggcat ccagcccccc
    13141 gaccgccgag ctgaatgtga tggcgacccg gggcgcgatg tcgcgcgtaa tttcaatccc
    13201 gccgttctgc aaagcgcgat gcacaaccag cagcgcgtat ttgaaaatgg gtgacgtcca
    13261 gtgcgccatt tctctgggct gcaaaaacgg atacggaccc ggatcgattt cctccacctc
    13321 gccggccacg cgtaccggaa aattttcact gagcgggaat ctggtcaacc ggccgatgcc
    13381 gctttttccg gcaaccgctt gccgccattg tgcatccagc tgcgccccca gtgacgatac
    13441 ggcatcataa cccacgatga cggttttttt caaccccatg ctccgacctt aaaaataaat
    13501 cgatatcccc tgaaaataaa ttaaaggggg attttgatag agggacctat atcaaaattt
    13561 tcgggggatt accacggaat aaagtgatag agcttggcga acatctcgta aacttcgatc
    13621 cagttttgca gatctttggt tgatttcaga tgttcacgct tgttgaccat gacccagctc
    13681 atatgcgccg cgccgtcttt gcaggtggag caatcgcgtg tcgcggacgt acagcagcga
    13741 tgaacggttt tcagatcgga tgcccagcgg gcaaaatgaa tcagccggcg gggacggttt
    13801 gctctgttat ccagggcttc ggtaacggac ggacattcat tccagccgaa acgtcgtccc
    13861 atcattttcc cggtggtaat gatttcatga tagtatttgc aggaaatgac cgtctgcgga
    13921 taggtgtcga gcatctcatc catttcgtga caaagatctt tgagctcgcc ctcctgccag
    13981 gaaaaaccgt cgacaccctc gtcattggat aaaagttgca agtggacttt caatccgaca
    14041 tcggcaatgc gtttaatgac cggttcaatt ttgccgacct gccgcggcgt aatggtataa
    14101 agataatagg tataggcatc cccttcgtaa tttttgctgg agacggcaaa cgtgtcgcgt
    14161 ccgcgcagaa ttttttcatc ttcagcgctg ccccaaagcg aaatgcccac catcatatcg
    14221 gggaatctgt cacgcggaac tttaatcaac ccgttggtgg cacaatacgt gggcagccgt
    14281 ttataaaacg cctcgacgcg gtccagacaa agcgtcggct caccgccgat cagaatcgcc
    14341 aggttcacgc ccctttcttt ttcgcgatcg ataaatgctt cccattttcc gatatccatt
    14401 tcttcgaccg ccgcctgatg ctcgcccgaa gaaaagaaaa aacaaccctt gcagcg
//
LOCUS       DAOOLB010000048        14330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4550_length_14330_cov_39.847356, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000048 DAOOLB010000000
VERSION     DAOOLB010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14330
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4550_length_14330_cov_39.847356"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="PLF77_07110"
     CDS             complement(<1..923)
                     /locus_tag="PLF77_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPC08658.1"
                     /translation="MFKEKIHLGLDLQGGMHLVLEIDTDKALDAMVERLSNDLKESLM
                     DSKVRFRNLEKMQPAKISMELAGADEEKGLENVLREHYPDLEIESASPREDGKFVTLR
                     INDKRAAELKKLTVEHSLETIRNRVDQFGISEPEIIPEGDDRILVQLPGIKDPERAKN
                     LIGKTALLEFKLVDDENSLDEALRGNIPEGDVIAYGLRADQSTGVRGSSPYLLKDKSL
                     MTGASLETAKVQIADRFGDVTVGLKFNSQGAVDFERITGENIRKRLAIVLDGVVHSAP
                     VIQERISGGQAQITGNFTMEEARDLAIVLRAG"
     gene            complement(1060..1419)
                     /gene="yajC"
                     /locus_tag="PLF77_07115"
     CDS             complement(1060..1419)
                     /gene="yajC"
                     /locus_tag="PLF77_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPC08659.1"
                     /translation="MTLTSLAYAMGGAPGGPAAGGGGDWGFIITMVIIFVIFYFLLIR
                     PQQKKQKELKAMLDNLAYGDMVMTTGGIHGKITGLTDAIVTLEIADKVRIKVARSAIG
                     AIVQKSGSAAPAAPTKS"
     gene            complement(1439..2563)
                     /gene="tgt"
                     /locus_tag="PLF77_07120"
     CDS             complement(1439..2563)
                     /gene="tgt"
                     /locus_tag="PLF77_07120"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPC08660.1"
                     /translation="MFRFNVIKKDDKTAARMGEINTARGIVQTPVFMPVGTQGTVKSM
                     LPEEIKNCGAQMLLSNTYHLYLRPGHETIKNLGGLHRFMNWPYPILTDSGGFQVYSLG
                     TLRKITPEGVMFRSHIDGSRHFLTPQKAIEIQEALGSDIMMCLDECTPYPATPSQTEK
                     SLNLTAKWALSCREAKTDNGQALFGIIQGGSYPDLRRQSLEKTVLLNFDGYALGGVSV
                     GEPKEIMYTITDVMAPLLPENKPRYLMGVGTPEDIVFGVSCGIDMFDCVIPTRCARHG
                     LLFTNSENVVIKNARWREDADPIDETCDCYTCRNYSRAYLRHLYFAGEILAMVLNTIH
                     NVRYYMRLMENIRLAIQENRFEAFKNAFLKDRTESKKHLT"
     gene            complement(2634..3671)
                     /gene="queA"
                     /locus_tag="PLF77_07125"
     CDS             complement(2634..3671)
                     /gene="queA"
                     /locus_tag="PLF77_07125"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPC08661.1"
                     /translation="MQLKDFSYDLPDELIAQHPSAKRDQSRLLLMDRHKDALADMSFF
                     QLPDLLKKGDVLVVNDSRVIPARLFGRKQTGGQLEILLLNVKKKEPTSQIWEVLIRPA
                     KRLHEGDDIRLAGHCEAKVLARISEKKWLVEFCAPDGFESYVNTFGRAPLPPYIKRKK
                     NARPDAADRDRYQTIYARQPGSIAAPTAGLHFSDEVIQQLKDRGIQIAHVTLHVGYGT
                     FLPITTDEVEKHVMEPEYYEISPESARIINNAERVIAVGTTSTRTLESVSNEDGYISA
                     RSGVTGLFIYPGYKFKRINGLLTNFHLPQSSLFLLVCAFAGTDLIKKAYAHAVKNRYM
                     FYSYGDCMLIL"
     gene            complement(3740..3925)
                     /locus_tag="PLF77_07130"
     CDS             complement(3740..3925)
                     /locus_tag="PLF77_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HPC08662.1"
                     /translation="MKDLLCVLGLVFIIEGLPYFVFPEKLKVYLAKIMTLPDSTLRFI
                     GISSMILGLILLYFGRR"
     gene            complement(3928..4704)
                     /gene="mazG"
                     /locus_tag="PLF77_07135"
     CDS             complement(3928..4704)
                     /gene="mazG"
                     /locus_tag="PLF77_07135"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPC08663.1"
                     /translation="MESLNRLIDLIRKLRAPDGCPWDRQQKKEDIGKYMLEEAYEAID
                     ALEKKNPEALKEELGDVLFQILFICEICAESGLFSLEDVMDGVNEKMIRRHPHVFGTV
                     KVTSVQDVKDNWQQIKKKERKETGREKSLFSSIPRSLPALKRAQKITALASQSGFDWK
                     DIRSVRDKLEEEWQEFEAAVRAENRIKTEEELGDILFTMVNLSRFLRVDAETALSKTT
                     EKFLRRFAHVTEQLSSRGLKPEEATLQQMDALWDEAKKKD"
     assembly_gap    5320..5419
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5645..6028
                     /locus_tag="PLF77_07140"
     CDS             5645..6028
                     /locus_tag="PLF77_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPC08664.1"
                     /translation="MTEKNGDVLKGLFIGGLIGIALGILFAPKSGKETRDDILLKTDE
                     MLARVKEEYEKALEKSRLAYEDSLNRLKGVNLSVKAKAGEAEGKISELAQQGSEALQS
                     NKNKLKKAIDAGLKAYKEELGKEKI"
     gene            6092..6505
                     /locus_tag="PLF77_07145"
     CDS             6092..6505
                     /locus_tag="PLF77_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HPC08665.1"
                     /translation="MTLEIALLILVGILIVLAVGCIPLLVQIWRISKDLEMTLQTFNQ
                     SLPLLLKNLEEITTNVNSSSSVINKKIQNFAHPSNMSGLLLGDIISNIHHFAPLALKL
                     PIFRIMRNVVSIAKGLRVFVEVLLNKEKDETLKQG"
     gene            6616..6975
                     /locus_tag="PLF77_07150"
     CDS             6616..6975
                     /locus_tag="PLF77_07150"
                     /inference="COORDINATES: protein motif:HMM:NF008448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HPC08666.1"
                     /translation="MKKTQQSSSGEDYREKSLAMNGLVCARCGREFPESKRHLLTVHH
                     KDGNHHNNPADGSNWENLCVYCHEDVHSREVLAEYLGGEAHGKEVSLVYNDAQGAVGS
                     GLLAEKLKTALKKKSDK"
     gene            complement(6978..7175)
                     /locus_tag="PLF77_07155"
     CDS             complement(6978..7175)
                     /locus_tag="PLF77_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HPC08667.1"
                     /translation="MRATYKDFDATELFCPRCKKAVAVRKRLLLILQEGEKYDYTCVH
                     CGTSIGDKLVKDSAPSGLVIN"
     gene            complement(7172..>8857)
                     /locus_tag="PLF77_07160"
     CDS             complement(7172..>8857)
                     /locus_tag="PLF77_07160"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPC08668.1"
                     /translation="NYREFYEARQFTPADAAFSSKIKLAGQNDIPFGADIIFKIANIK
                     HFSFFIEICEDLWVPVPPSSFAAMAGATVIGNLSASNITIGKSEYRQQLAANQSARCV
                     SAYLYAAAGPGESTTDLAWDGHAMIHENGNLLAESPRFSQQAQIIYSDIDLDRLAQDR
                     MRLTSFSKNAAAHKEKILSFRKTEFDVDVPTGKILLTREYPRFPYIPADPSKRDQRCY
                     EAYNIQVQGLAKRLKASGIPNIVIGVSGGLDSTHALIVAARTMDTLKLPRKNVKAYTL
                     PGFATSRKTYNNALKLMTALGVESNEIDIKPACRQMLKDIKHPYANGKQKFDITFENV
                     QAGQRSAHLFRLANMRNALVVGTGDLSELALGWSTYGIGDHMSHYNVNASVPKTLIQH
                     LIRWVAQTGQFSQDVSDILIDVLKTEISPELIPGKNKDHLVQKTEDTIGPYELQDFNN
                     FYISRFGYLPSKVAFLAYHAWKDRNKGLWPDNIAREKKNAYSLKEIKKWLHVYLYRFF
                     KTSQYKRSCIPNGPKVGSGGSLSPRGDYRAPSDSEAEIWLQNWATIPEEDIRS"
     assembly_gap    8858..8957
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9123..9890)
                     /gene="folE2"
                     /locus_tag="PLF77_07165"
     CDS             complement(9123..9890)
                     /gene="folE2"
                     /locus_tag="PLF77_07165"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HPC08669.1"
                     /translation="MIDIQSQKDSRNIDIKKVGVKNIKYPVIVLDRAKGTQLVNATIN
                     MYVNLPHHFKGTHMSRFVEALNEFKGQINIKTFRTILEKMRQKLHAHSAHMEIEFAYF
                     LEKTAPVSKAKSLMEYRCIFAGESNAETSDFLVGVIVPVTTVCPCSREISQYGAHNQR
                     SVVTTKVRFKKFFWIEDLIRIVEDSASGEVYSLLKRVDEKYVTEKAFENPKFVEDVVR
                     SVAVKLNKDDNFTWYCIEAENFESIHNHSAYALVEKG"
     gene            complement(9921..10289)
                     /gene="queD"
                     /locus_tag="PLF77_07170"
     CDS             complement(9921..10289)
                     /gene="queD"
                     /locus_tag="PLF77_07170"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPC08670.1"
                     /translation="MYEVTIIKSFSAAHLLADIGGKCETLHGHNFKVEVSVAADQLDA
                     NGLLIDFRFLKKILGEILDDIDHKHLNALAHFKGINPSAENIAKYIYDQMDEKVKTAG
                     VHMVRVKIWESENAAVSYTR"
     gene            complement(10431..11822)
                     /locus_tag="PLF77_07175"
     CDS             complement(10431..11822)
                     /locus_tag="PLF77_07175"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08671.1"
                     /translation="MKILLVRPPSFKMPIIIPNLGLGYLASVLRHNGHTVSLLDCAKT
                     KTTHRQYRDCLMDEQPDIVGIQMHTCDFSSARQCIEIAKTVNGKTVTIVGGPHPSGDP
                     EGTMEIPGLDYAFVGEAESGLPRLLEYTRSAGQVAIDSIDGLVHRQNGKIIKNKKGAI
                     QNPDDIPFPGWDLIDPQTYPAAPHGTFSKSLPVAPVITSRGCPFQCTYCGANVNTGRH
                     FRKRSVENIVEEIKYLQKTFGIREFHIEDDNFTLDKARVMAFCHKLKEENIKLHWACT
                     NGVRLDTLDKELLQNMESAGCYSLAAGIESGSPRIINDMKRNETLDIMIEKINLIARE
                     TKIRITGFFMIGYPEETRNDIEKTIELAMKLPLHRAQFSNFLPLPGTEIYDRLIQRKE
                     IAPDTLPWDLYQNNRVVYTPKNISPREMRRLMRKAFAAFYFRPRILWGLLKEIHSPGQ
                     FRTVVRRFFDVFR"
     assembly_gap    12069..12168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12169..13418)
                     /locus_tag="PLF77_07180"
     CDS             complement(<12169..13418)
                     /locus_tag="PLF77_07180"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPC08672.1"
                     /translation="MPPILLLCFVSICFFSQFSNSVTVDEFVHFPAGIYILKTMDWRM
                     DHPAPPLVKCFPAVSAIFTHPEIETKLFQKYPDLYGLGYRFMEQNKDRYRSIFTYGRS
                     MIIFLGFLLGITIYFWGKELYGYKGALFALILYAFNPNILAHASLITMDIGVTLTIFL
                     SVYGLWKYFKKPNAFSIVIAGVALGLAQLSKFTALLLYPVYFFIFGTLCIISLCKRKD
                     EGIKSSVMTISKYGKDFLMIIFISIFMINLGYLFSGSFMPLSEYNLSSRLFNNLASLL
                     WSGLPVPLPDHYISGFDSQLMQSVGGNPFYIGYLMGEHSMSGWWYYYIVAFLVKNPVS
                     WLIILCLTVIAWLEIKIARNDIITFLCISMPVIFYCIYFSFFTNIPIGIRYLLPIFPF
                     LFLASGFLFHQAIIKFKRIQYLLM"
     gene            complement(13506..>14330)
                     /locus_tag="PLF77_07185"
     CDS             complement(13506..>14330)
                     /locus_tag="PLF77_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC08673.1"
                     /translation="IAVMIAWGLSVLMTNDYRSKRILSVAGVAFMVIMTLLTWKQCGY
                     WKDNPALFTHALKVTKNNYIAHDYVALSLFAEGKYEKAKDHFDKALIINPDYVETYNN
                     RGMLYAETGHYEKALVDFNRAIRMKSDYADAYYNRGLTYNRLGHHQQAFDDYSRAILL
                     KPEHTKAYNNRGIFFFKLGDYQSAIQDFGQAIKLKTDYAEAYFNRGITYAKIGKGHRA
                     LEDFSNAIRIEENFTHAYGNRAAVHFKQGNHKLGCADARKACALGNCTILQSADCR"
BASE COUNT         3539 a         3214 c         3400 g         3877 t
ORIGIN      
        1 ccggcccgca aaacaatggc gagatcgcgc gcttcttcca tggtaaagtt cccggtaatc
       61 tgcgcctgac cgccggatat tctctcctga atgacgggcg cggaatgaac aacgccgtcc
      121 agaacaatgg ccagcctttt gcggatattt tccccggtga ttctttcaaa atcgacagcg
      181 ccctgagaat tgaatttcag gccgacggtc acatcaccga aacggtcggc aatctgaacc
      241 ttggccgttt cgagggaagc gccggtcatc agggacttgt ctttgagcag atagggagaa
      301 gagcctctga ctccggtcga ttgatcggcg cgcagaccgt aggcgatcac atcgccttcg
      361 gggatgttgc cgcgcagggc ttcatccagc gagttttcgt catcaaccag tttgaattcc
      421 agcagggcgg ttttcccgat cagatttttt gccctttcgg ggtctttaat gcccgggagc
      481 tgaacaagga tccggtcgtc tccttccggg atgatttccg gctccgagat gccgaactga
      541 tccactctgt tccggatcgt ttccagagaa tgttccacgg ttaatttttt caattcggcg
      601 gcgcgtttgt cgttgattcg taacgtgaca aactttccgt cttcccgcgg actggcggat
      661 tctatttcaa gatcggggta atgttcgcgc aggacgtttt ccagcccctt ttcttcatcg
      721 gcaccggcca gctccatgga aatttttgcc ggttgcattt tttccagatt gcggaagcgc
      781 accttgctgt ccatcagaga ttcttttaaa tcgttggaga gtcgctcgac catggcgtcc
      841 agtgctttat ccgtgtcgat ttcaagaaca agatgcatgc cgccctgcaa gtccagcccc
      901 agatgaattt tttctttaaa catgtactta tcccaggggg aggggatatt ggaaaaaaat
      961 gtcggaatca gaaaataaag acagccgaga cacacgatta aagttatagc tcctctgagc
     1021 tttaaggatt tgaacatgaa aatgcccctt tcttaaaaat tatgacttgg ttggcgctgc
     1081 cggcgctgcc gaaccggatt tctgaacaat ggccccgatg gcgctgcggg cgactttgat
     1141 tctgaccttg tcggcgattt ccagggtaac gattgcatcc gtcaatccgg tgattttgcc
     1201 gtggatgcct ccggtcgtca tgaccatatc gccgtaggcc agattatcca gcatggcctt
     1261 gagttccttt tgtttttttt gctgcgggcg aatcagcaga aagtaaaaaa ttacaaaaat
     1321 gatcaccatg gtgatgatga atccccagtc tccgcctcct ccggccgccg ggccgccggg
     1381 cgctccgccc attgcatatg ctaacgatgt caatgtcata tcctccttgc gatttgctct
     1441 atgttagatg ttttttcgat tcggtcctgt ccttcagaaa agcgttttta aatgcttcaa
     1501 aacggttttc ttgtatggcc agccttatat tttccatcag tctcatataa taacggacat
     1561 tatgaatggt gttcaaaacc attgccagta tttcaccggc gaaataaaga tgtcttaaat
     1621 atgcccggga ataattccgg caggtatagc aatcacacgt ttcatcaatc ggatcagcgt
     1681 cttcccgcca gcgggcgttt tttataacaa cattttccga atttgtaaac aataatccgt
     1741 gacgggcgca ccgggtggga ataacgcaat cgaacatgtc aattccgcag gatacaccga
     1801 aaacaatgtc ctccggcgtg ccgacgccca tcagataacg gggtttattc tccggcagca
     1861 ggggagccat gacatcggtt atggtataca tgatttcctt gggctcgccg acgctgacgc
     1921 ctcccagagc gtagccgtcg aagttcagca aaacggtttt ttccagggat tgcctgcgca
     1981 gatcaggata cgagcctccc tgaatgatcc cgaaaagcgc ctgtccgttg tctgtcttcg
     2041 cctcccggca tgacagggcc cattttgcgg tcagattcag cgatttttcg gtttgggacg
     2101 gtgttgcggg atacggcgtg cattcgtcga ggcacatcat gatatccgat cccagcgctt
     2161 cctggatttc gatcgctttc tgcggtgtga gaaaatgtct ggatccgtca atatgcgaac
     2221 gaaacatgac gccttcgggg gtgatttttc gcagggttcc gagactgtat acctgaaaac
     2281 cgccgctgtc ggtcaggatc ggataaggcc agttcataaa acgatgcagt ccccccaggt
     2341 tttttattgt ttcatgaccg ggacgtaaat ataaatgata cgtgttgctc agaagcattt
     2401 gcgcgccgca gttttttatc tcttccggca gcatggattt gaccgttccc tgcgtgccga
     2461 ccggcataaa aaccggcgtc tgcactatcc cgcgggcggt gttgatctct cccatgcggg
     2521 cggccgtctt gtcgtctttt ttgattacgt taaagcgaaa catttttatg tcctggctcc
     2581 ggtaaacggg attaacgcgt gcacgcattt gcttcccgta aaaaatggcg gtattaaagt
     2641 atcagcatgc aatcgccgta gctgtaaaac atataacggt tttttacggc atgcgcatac
     2701 gcttttttga tcaaatcagt gccggcaaaa gcacagacca gcagaaacag cgaggattgc
     2761 ggcaaatgaa aattggtcag caggccgttg attcttttaa atttataacc gggataaata
     2821 aataaaccgg tgaccccgga tcgggcgctt atatatccgt cctcgtttga aacgctttcc
     2881 agcgtgcggg tggatgtggt gcccaccgca ataacgcgct cggcgttgtt gatgatcctg
     2941 gcgctttccg ggcttatttc ataatattcc ggttccatga catgtttttc aacctcgtcc
     3001 gttgtaatgg ggagaaacgt gccgtaaccc acatgcaagg tgacgtgagc aatttggatg
     3061 ccccggtctt tcaattgctg tatgacctcg tctgaaaaat gcagaccggc tgtcggagcc
     3121 gcgatggagc cgggttgccg tgcgtaaatg gtctgataac gatcccgatc ggcagcatca
     3181 ggccgggcat ttttcttcct ttttatatag gggggaagcg gggccctgcc gaaggtgttc
     3241 acataggatt caaagccatc cggagcacaa aattccacca gccatttttt ttcggaaatc
     3301 ctggctaaaa cttttgcctc gcaatgaccc gccagacgga tatcgtcccc ttcatgcagc
     3361 cttttggccg gtctgatcag cacttcccat atctgggagg tcggctcctt ttttttcacg
     3421 ttcaaaagca gaatttccag ctgtcccccg gtttgctttc tgccgaacag tctggccggg
     3481 atgacccggg aatcgttcac gacgaggacg tcgccttttt tcagcaagtc cggcagctga
     3541 aaaaaggaca tatccgccag ggcatctttg tgcctgtcca tcaaaagcag gcgggattgg
     3601 tcgcgcttgg cagagggatg ctgcgcgatc aattcatccg gcagatcata tgaaaaatct
     3661 tttaattgca tagggctgtc gtcatgaaaa agtgctggca ggtaaccaga ttgtgatttt
     3721 aatgtcaatg ccattgtttt cagcgtctgc cgaagtacaa aagaatcaaa ccgagaatca
     3781 tggacgatat tccgatgaac cgcaacgtcg aatccggcaa ggtcattatt ttggcgaggt
     3841 atacttttaa cttttccggg aaaacaaaat acggaagtcc ttcaataata aagacgagtc
     3901 ccagcacgca aagcaaatcc ttcattttca atccttcttt ttcgcttcat cccataaagc
     3961 gtccatctgt tgcagggttg cttcttccgg ctttaagccg cgggatgata gctgttctgt
     4021 gacatgagcg aaacgccgca gaaatttttc tgtcgttttg gatagggcgg tttccgcgtc
     4081 aacacgcaga aaccggctta aattgaccat cgtgaataat atgtcgccca gttcttcctc
     4141 cgttttgatg cggttttctg cccttacggc agcctcaaac tcctgccatt cttcttccag
     4201 cttatcccgg acactccgga tgtctttcca gtcgaagccg gattgcgatg ccagggccgt
     4261 aattttctgc gcccttttca gagccggaag cgagcgcggt atgctggaga acagactttt
     4321 ctcccggccg gtttccttgc gctccttttt tttgatctgc tgccagttgt ctttgacatc
     4381 ctgcacggag gttactttga cagtgccgaa tacatgggga tgccgccgga tcattttttc
     4441 gttgacgccg tccatgacat cctcaaggga gaacaagcct gactcagcgc atatttcgca
     4501 aataaataat atctgaaaaa gcacatcgcc cagttcttcc ttcagggcct ccggattttt
     4561 cttttcaaga gcatcaatgg cttcatatgc ttcttccagc atgtatttgc cgatgtcttc
     4621 ttttttctgt tgacggtccc aggggcagcc gtcgggtgcg cggagctttc tgatgagatc
     4681 aattaatcgg ttaaggcttt ccatgttcct cgttcctctt gctttttcat gaatgcacag
     4741 cttgtcttta tgacgtcaac gtttccaagc ccgttttata tatcatattc gatcagccgg
     4801 taatatacac aaaaatggtt ttctgaattt tacagaatta aaggaaggaa tgattgtctc
     4861 ctttaagaag gagccattgt tttgcggcgg acggcggctc atttctattg catagacgaa
     4921 agttaatatg aataacaata atatcaagaa tatgcatgcg tggctgctct ttttctgagt
     4981 tgcaggaaag gagcatttct tcgcataaaa tattgaaaaa aagcgtaaaa aatggtgaaa
     5041 attattcttg acaaggcaaa taaaaagcgt attcttcccc ttcaatacag acaacaggct
     5101 tttaaaaaag ccgtttatta tgaaatcttt gaaaggaggt tagccgttaa tgaaaaaaat
     5161 atttgcgatt tttgttgcta tgctttttgt tgcttctttg tctcttgcag tcgtaggctg
     5221 caaaaaagct gaagaacctg cagtcgaagc tcctgcagta gaagcaccgg ttgctccggc
     5281 acctgaagca gcgcccgcgc ccgctcctga agcagcgccn nnnnnnnnnn nnnnnnnnnn
     5341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnng cacctgctcc cgaagcagca cctgcacctg cacctgcaaa
     5461 ataattgcat cgcatccaaa agcaggatag attgatcgga cattccccga aaggaatgtc
     5521 cgattttttt tctgaagaag tttaattttt aaaaatatgt tatactcagt aaaagcaaag
     5581 tttataatca ttatcctgtc aatcgagctt tggggtattt acttttttat aaggaaggtg
     5641 ctttatgacg gaaaaaaacg gtgacgtatt aaaaggtctg tttatcgggg gcctgattgg
     5701 aatcgctctg ggtattttgt ttgctcccaa aagcggcaaa gaaacacgtg atgatatttt
     5761 actcaaaacc gacgaaatgc tggctcgtgt gaaagaggaa tatgagaagg cgctggaaaa
     5821 gagcaggctt gcttacgagg attctctgaa ccgtttgaag ggcgtcaact tatccgtgaa
     5881 agcgaaggcg ggggaggcgg aagggaaaat cagcgaactc gctcagcagg ggtctgaggc
     5941 cctgcagagc aataaaaaca agctcaaaaa agcgatcgat gcgggcctga aagcatataa
     6001 agaagaactc ggtaaagaaa aaatctgacc ctgtctggct tataaacatc ataacaggat
     6061 tcgaaaaaac gatctttaaa ggagaaaatc aatgactctg gaaatcgcct tgttaatttt
     6121 ggttggcatt cttatcgttt tggccgtcgg ttgtattccg cttcttgtac aaatctggcg
     6181 catatcgaaa gatctggaga tgacactgca aacgttcaac caaagcctgc cgctgttatt
     6241 gaaaaatctg gaagaaatta ccacgaacgt caacagctcc tcctcggtca tcaacaaaaa
     6301 gattcaaaac tttgcccatc cctcgaatat gtcaggtctg cttctggggg acattatcag
     6361 taatatccat cattttgcgc cgctggcgct gaaattgccg attttccgta ttatgagaaa
     6421 tgttgtgtcc attgccaaag gcctgcgtgt ttttgtggaa gtccttttga ataaagagaa
     6481 agatgagaca ttgaagcaag gttaacgatg ggcaaggtaa cgttttccag taagtggggc
     6541 agtgtaaagg ttcagaaaaa gccgactgcg tcttcgtccg aatcggctaa accgatggaa
     6601 gcactggtcc gggaaatgaa gaagactcaa caatcttcat cgggagaaga ttaccgggaa
     6661 aagtccctgg cgatgaacgg ccttgtttgc gcgcggtgcg gccgggaatt tcccgaatca
     6721 aaaagacatc ttttaaccgt ccatcataaa gacggtaatc atcacaacaa tcccgccgat
     6781 ggaagcaact gggaaaatct ttgtgtctat tgccacgaag atgttcacag ccgggaggtt
     6841 ctcgcggaat atctgggtgg agaggcacac ggcaaggaag tgagcctggt ctataatgat
     6901 gctcaggggg ctgttgggtc cggattgctg gcggaaaaac tcaaaaccgc cctgaaaaaa
     6961 aaatcggata aataatttca gttgatgacc agaccggaag gggcagaatc tttgaccagt
     7021 ttgtcgccga tggatgttcc gcaatgcaca caggtgtaat cgtacttttc accttcctga
     7081 agaatcagca gcaatctttt tctgacggct accgctttct tgcagcgggg acaaaacaat
     7141 tcggtggcgt caaaatcttt ataggttgct ctcatgatcg aatgtcctcc tccggtatgg
     7201 ttgcccagtt ttgcagccag atttcggcct cgctgtcgct tggcgcccgg tagtcgcctc
     7261 gcggcgaaag cgagccgccg gatccgacct tggggccgtt ggggatgcag gagcgtttgt
     7321 actgcgatgt tttaaagaat cgatacaaat aaacgtgaag ccattttttg atttctttca
     7381 acgaataggc gtttttcttt tcccgggcaa tattatcggg ccagagacct ttgtttctgt
     7441 ccttccaggc gtgatacgcc agaaaagcca ccttggaagg cagataaccg aatcgggaaa
     7501 tatagaaatt attaaagtcc tggagttcat acgggccgat ggtgtcttcc gttttctgga
     7561 cgagatgatc tttgtttttc cccggaatca gttcggggga tatttccgtt ttcagcacat
     7621 cgattaaaat atcggaaaca tcctgtgaaa attgcccggt ctgggccacc cagcggatca
     7681 gatgctgaat cagtgttttc ggaacgctgg cgtttacgtt gtaatgagac atgtgatcgc
     7741 cgatcccata cgtgctccag cccagcgcca gttcgcttaa atcgcccgtg cccaccacca
     7801 gtgcgttgcg catgttggcc agacggaaaa gatgcgctga acgctgtccg gcctgaacat
     7861 tttcaaaggt gatatcgaat ttttgcttgc cgttggcata cggatgtttg atgtccttga
     7921 gcatttgacg gcaggcaggt ttgatatcga tctcattgga ctcgacgccg agggccgtca
     7981 tgagtttcag ggcattgttg taggtttttc gcgatgtggc gaatcccggc aaggtatacg
     8041 ccttgacgtt tttccggggc aatttcaggg tatccatggt ccttgcggcg acaatgaggg
     8101 cgtgcgtgga atccagaccg ccggaaacac cgatgacgat attgggaatg ccggacgctt
     8161 tcagccgttt ggccaacccc tgtacctgta tattataggc ttcgtagcat cgctgatccc
     8221 gctttgaagg atcggccgga atatacggaa agcggggata ttcgcgggtc agcagaattt
     8281 tacctgtcgg cacatcgaca tcgaattcgg tcttacggaa ggataaaatt ttctctttat
     8341 gcgcagcggc gtttttactg aaggacgtca ggcgcattct gtcctgagcg agacggtcga
     8401 gatcgatgtc gctgtagatg atctgcgctt gttgtgaaaa acgcggtgac tcggcgagca
     8461 ggttgccgtt ttcgtgaatc atggcgtggc cgtcccaggc caggtccgtt gtcgattcgc
     8521 cgggacctgc cgcggcgtaa agataagcgg aaacgcaacg tgccgactga ttcgccgcca
     8581 gttgttgacg gtattcggat ttgccgatcg tgatgttgga ggccgataga tttccgataa
     8641 cggttgctcc ggccatagcc gcaaaactcg agggtggaac cggaacccac aagtcttcgc
     8701 agatttcgat aaaaaaactg aaatgtttaa tattggcgat tttaaaaatg atatcggcgc
     8761 caaaagggat atcgttctgc ccggccagtt taatttttga agagaaagcg gcgtccgccg
     8821 gtgtgaactg gcgggcttca taaaattcgc gataattnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnngcc agaatggctt tctgttgcgc cgcctggcgg gccagttcga
     9001 tggttttctt cgcgttgaaa acgggatcgg ctactttgac ttccggtatg cagaccgccg
     9061 cgcgaataaa gccatgctga tacaggttga aaaaattttt cgatttgttc gccggcatgc
     9121 cattacccct tttctacgag agcgtacgcg ctgtgattat gaatgctttc aaaattttcc
     9181 gcttcaatac aataccaggt gaagttgtca tccttgttca atttcacggc gacactgcgg
     9241 acaacatctt ccacgaattt cggattttca aaggcctttt ccgtaacgta tttttcgtca
     9301 acgcgtttca gaagagaata cacttcgccg ctcgcggaat cttccactat tctgattaaa
     9361 tcttcaatcc agaaaaattt tttgaagcgg acttttgtcg tcacgacact tctttgattg
     9421 tgggctccgt attggctgat ttcgcgggag caggggcaga cggttgtgac cgggacaatc
     9481 actcccacga gaaaatccga tgtttcggcg ttgctttctc cggcaaagat acaacggtat
     9541 tccatcaggc tctttgcctt ggaaacgggc gccgtttttt ccaggaaata agcaaattca
     9601 atttccatgt gggcggaatg ggcatgcagc ttctgccgca ttttctccag aatagtccgg
     9661 aacgttttaa tattgatctg gcctttgaat tcattgagcg cttccacaaa gcggctcatg
     9721 tgcgttccct tgaagtggtg cggcaggttc acatacatgt tgatcgttgc gttgaccaac
     9781 tgcgttccct tggcccggtc aaggacaatc accgggtatt tgatattttt tacgccgacc
     9841 tttttaatat caatattgcg agaatctttc tggctttgaa tatcaatcat ggcactatgc
     9901 tcttttcatc attggcgttc ttaccgggtg tacgacacgg cggcgttttc cgattcccag
     9961 attttgaccc gaaccatgtg cacaccggcc gttttgactt tttcatccat ctgatcgtaa
    10021 atatattttg ctatgttttc cgccgaggga ttgatgcctt taaaatgggc cagcgcgttg
    10081 aggtgtttgt ggtcgatatc gtcaagaatt tcccccagga ttttttttaa aaaccggaaa
    10141 tcgatcaaaa gaccgttggc gtcgagttga tcggctgcca cggatacttc aaccttgaaa
    10201 ttgtgaccgt gcaatgtttc gcatttgccg ccgatgtcgg ccagcagatg tgcggcggaa
    10261 aatgatttta ttatcgtaac ttcatacatt ggtcatgtgc tccgtttctt aaagtagaat
    10321 tattatcata ttttcaagag atgttgaatc attaattatt gacaaccgat aatatgaagc
    10381 atcgttgcgt cctctgaaat gaagggtgca gatttcatca gggggaatat ctatctgaaa
    10441 acatcaaaaa aacggcgaac gactgtacga aactggcccg gggaatggat ctccttgagt
    10501 aatccccaaa gaatacgggg acgaaaatag aacgcggcga aggcttttct catcagtcgt
    10561 cgcatttccc ggggcgaaat atttttcggc gtatagacga cgcgattatt ctggtagaga
    10621 tcccagggca gggtatcggg agcaatttct ttcctttgga tgagccgatc atatatttct
    10681 gttcccggaa ggggaagaaa attgctgaat tgggctctgt gcaaagggag tttcatagca
    10741 agttcgatgg ttttctcaat atcatttctc gtttcctcag ggtagccgat cataaaaaaa
    10801 cccgttattc tgatctttgt ttcccgggca ataagattga ttttttcaat catgatgtcg
    10861 agtgtttcat ttctcttcat gtcgttgatg attctgggag aaccggattc gattcctgcc
    10921 gctaaagaat aacacccggc gctttccatg ttctgcagca attctttgtc cagggtatcc
    10981 agtctgacgc cgttggtgca ggcccagtgc agcttaatat tttcttcctt gagtttatgg
    11041 cagaatgcca tcacacgcgc tttgtccagc gtgaaattat catcctcgat gtgaaactcc
    11101 ctgatgccga atgtcttttg cagatacttt atttcttcaa caatgttttc gacacttctt
    11161 ttccggaagt gtctgcctgt gtttacgttc gcaccgcaat aggtgcattg aaaaggacac
    11221 cctctgctgg ttatgacggg cgctacaggc agactttttg aaaacgtgcc gtgcggcgcc
    11281 gccggatagg tttgcgggtc gatcaaatcc caaccgggaa aggggatgtc atccgggttt
    11341 tggatggccc cttttttgtt ttttatgatt tttccgtttt ggcgatgaac caatccgtcg
    11401 atggaatcga ttgcaacctg accggcagac cgggtatatt ccagcaatcg gggaaggccc
    11461 gattcggctt ctcccacgaa agcataatcc agaccgggaa tctccatggt tccttccgga
    11521 tctcccgacg gatgcggacc gccgacgatg gtaacggttt tgccgtttac agtcttcgca
    11581 atttcaatac attgtctggc cgaggagaaa tcgcaggtgt gcatctggat gccgacgatg
    11641 tccggttgtt catccatgag acaatcccga tattgtcggt gggttgtctt tgtcttcgcg
    11701 caatcgagaa gactgaccgt gtgcccgtta tgcctgagaa ccgaagcgag gtatcccagt
    11761 ccgagattgg gaatgatgat cggcatttta aaactcggcg gacgtacaag cagaattttc
    11821 atatcattga actctgtaat ggattcattg cttcatgctc atcgttatgt tgacggtgtt
    11881 catctccgtt tcgaatgaat gatgactatt catcgatcgc gttgctcccc tgaagatcgt
    11941 gtggaaatat tttttctcag atcgaaaatg taaattgaat gaccgacaac ggcagacggc
    12001 tcgagatacc tgtattgatc atagtagttg atgtcgatgt acagcaattc gtgcgtgttt
    12061 tcctttaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa catcagaaga
    12181 tattgtatgc gtttaaattt tatgatggcc tgatgaaaaa gaaagccgga tgccagaaac
    12241 agaaagggga atattggcag caaatatctg atgccgatgg gaatgtttgt aaaaaaagag
    12301 aaataaatac aatagaagat caccggcatc gatatgcaga gaaacgtaat gatatcattg
    12361 cgtgctattt taatttccag ccaggcgatg acggtaaggc ataaaatgat gagccatgag
    12421 accggattct tgacgagaaa agcaacaatg taataatacc accagcccga catcgagtgc
    12481 tctcccatca gataaccgat ataaaaaggg tttccaccga cagattgcat taactgggaa
    12541 tcaaaccctg atatataatg atcaggcagc ggaaccggaa gtccgctcca tagtaatgag
    12601 gcgagattat tgaaaagccg gctggaaaga ttatattcag ataaaggcat aaaagaaccg
    12661 gagaacaaat agccgaggtt aatcataaaa atgcttatga aaatgatcat gagaaaatct
    12721 ttgccgtatt tgctgatggt catcactgaa gattttatcc cctcgtcttt tcttttacag
    12781 aggctgatga tgcataaggt tccaaagatg aaaaagtaaa ccgggtaaag cagcaacgcc
    12841 gtaaacttac tgagttgcgc cagacccaag gcaacaccgg caatgacaat cgaaaaggca
    12901 ttcggttttt taaaatattt ccacaggcca taaacgctga ggaatattgt cagtgttaca
    12961 cctatatcca tggtaatgag agaggcgtga gccaggatat tgggattaaa agcatataaa
    13021 attagagcaa acagggcgcc tttgtatcca taaagttctt tcccccaaaa ataaattgtg
    13081 atccccagta gaaaacccag aaaaataatc atactgcggc cgtaggtaaa gattgaccgg
    13141 tatctgtctt tgttctgttc cataaagcgg tatcccagac cgtataaatc cggatatttt
    13201 tggaataatt ttgtttctat ttcgggatgc gtgaagattg cgctgacagc agggaaacat
    13261 tttaccagcg ggggcgccgg atgatccatc cgccagtcca tcgtttttaa aatatatatg
    13321 ccggctggaa aatgaacaaa ttcatccacc gttaccgagt tagaaaactg gctgaaaaaa
    13381 cagatagaaa caaaacataa aagcagaatg ggcggcatta tttttcttaa gatgttcttg
    13441 gcagcggagg atgataagat gttattcatt acgcttgtca atttcatact ccttctgacc
    13501 ggaaatcaac gacaatcagc ggattgcagg atggtgcaat ttcccagagc gcatgctttc
    13561 cgcgcatcag cacagcccag tttatggttg ccctgcttga agtgaaccgc ggctctgttg
    13621 ccgtatgcat gggtaaagtt ttcttcaata cgaatcgcgt tgctgaagtc ttcaagagcg
    13681 cgatgccctt tgccgatttt tgcgtaagtg atgcccctgt tgaaataggc ttcggcataa
    13741 tctgtcttca atttaatcgc ctgaccaaaa tcttgaattg ccgactggta atcgccaagt
    13801 ttaaagaaaa aaattcctct gttgttgtag gctttggtat gttcaggctt cagaaggatt
    13861 gctctgctgt aatcatcaaa tgcctgttga tgatgtccga gacgattgta agtcaatccc
    13921 ctgttgtaat aagcatcggc gtaatccgat ttcattctga tagcccggtt aaaatccacc
    13981 agagcttttt cgtagtggcc tgtttctgca tacaacattc ctctgttatt gtacgtttca
    14041 acataatccg ggttgataat gagcgctttg tcaaagtgat cctttgcttt ttcgtatttg
    14101 ccttcagcaa aaagagataa agcgacataa tcgtgagcga tataattgtt ctttgtaacc
    14161 ttcaatgcat gagtgaataa agcgggatta tctttccagt aaccgcactg cttccatgtt
    14221 aataatgtca tgatgaccat aaaggcgaca ccggcaacgg acaggattct tttgcttctg
    14281 taatcattcg tcattaaaac agacaatccc catgcgatca tgacagcaat
//
LOCUS       DAOOLB010000049        14205 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4611_length_14205_cov_24.884382, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000049 DAOOLB010000000
VERSION     DAOOLB010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14205)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14205)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14205
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4611_length_14205_cov_24.884382"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PLF77_07190"
     CDS             <1..215
                     /locus_tag="PLF77_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08674.1"
                     /translation="EGTVTTKYENNIAHLKITGLNFRHNYFEYLIMGFFQKSLKMFGK
                     KNVPKRIRSRASGDDDVYFQFELKDS"
     gene            complement(393..1238)
                     /gene="ccsB"
                     /locus_tag="PLF77_07195"
     CDS             complement(393..1238)
                     /gene="ccsB"
                     /locus_tag="PLF77_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HPC08675.1"
                     /translation="MNHTLILSYVTFIYFASFVFYLFRMILKKEFWGYAASLLAWLGL
                     FAHTLAMMLRWRASYDLGFGHVPITNLYESLVFFAWSIIMLYLAIEWRIKSRILGTFV
                     VPVAFFVMAYASIAPGVDHSIEPLIPALQSNWLTTHVLTCFMGYAAFTVAFGCGLLYL
                     WTEKKETRETNPASHPKENSSVLNTLDVLIYQSTSLGFIFLAMGIITGSVWAHYAWGS
                     YWSWDPKEIWSLITWLIYAIMLHARFVRGWRGRRMAMMTIVGFVSVLFTYLGVNFLTS
                     LHNYI"
     gene            complement(1235..2572)
                     /locus_tag="PLF77_07200"
     CDS             complement(1235..2572)
                     /locus_tag="PLF77_07200"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HPC08676.1"
                     /translation="MKKANFIWSFLASVKLAIVLLFFMVVIFIVATLVPPQRMDSEWL
                     WLNDLYHSKMFYVLMGLFSLNLMVCSIQRIPFAIKQYKTPSFPPPSGLFENLAPNRII
                     VTDKKMETVSRAVESLLKSKLFSVKETKLEKGKMLYRESGRLSLFGVYVVHLGVLMII
                     AGMVIGSVFGLEADLRLSEGEETNIIQLRNGEGEHPLDFSVRCDKFVVEFYDTGVPKT
                     YRSELSFIKNGQVVRQGSVLVNHPLSFDGLRFYQASYGVSEKSRARLTYSNAGAESPE
                     ISAGQGDTFDLPVQKAKATVLRVEENMMQFGPAVKLNIETDKRNIQFWVFQHIQDIAD
                     VNPGLFSAVPLFNPGLFKPLVFSLRGIEGKYYTGLQVVRDPGVPFVLAGGLILIAGMM
                     VIFFIAYRRIWFLLEQDPAGVRIRVAGRSHRYNEALQRRLDDLCAWMEKELAA"
     gene            2764..4032
                     /gene="nrfD"
                     /locus_tag="PLF77_07205"
     CDS             2764..4032
                     /gene="nrfD"
                     /locus_tag="PLF77_07205"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HPC08677.1"
                     /translation="MADSGKKRFLPHIKSAFERVLSGGWIYLLFVGLFGIFLLIGIGA
                     GIHALFIAGSRHAYATSREIPVAILIATYVFFVVASTGLCLVSSLGHVFGVENFLPLA
                     KRCVYLAIITILSGFLVIGLEIENPFRMAIYNVISPNFSSNIWWMGTLYGFAVVFLTI
                     EFILLFNEAHHHKAVIAGFLGVITEVAANSNLGGVFAMMHGRPYWYGPYFPVYFILSA
                     LITGCAFILFFFFLAHKAVNKKPFMESTQTALKAVSKLTLLLLAVMMIFTAWKVLTTS
                     VGSPAAAAAALSVLTEGPYSFNFWTIEILVGMVIPFVLILASRGRNLTVMFIAAVMMI
                     FSLFFTRLDMVVVGQIVPQYFEVGVVEYSKLHTYSPSIHEILVVLGGISFCLLAFLLG
                     EKVFSGFADSPQTDIMKEKTTDVAGVEELT"
     gene            4029..4766
                     /locus_tag="PLF77_07210"
     CDS             4029..4766
                     /locus_tag="PLF77_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPC08678.1"
                     /translation="MTDKASHKNIPESPLDAERRAFLKIGLKITGVLAGGSILSLVSN
                     INKAYSLDLFREKYPYKPHYAMVLRQDRCIDCEKCMKACIETNHVPDYGWRTKILKRD
                     LSSALDQKWEFIPILCNQCNNPPCVRTCPTTATYKDKTNGIVQMNNKKCIGCKSCIMA
                     CPYDARYYNEEKGAIDKCDFCFESRLSKGINTPACVEACPSDVRIFGDLSDPSSRVFQ
                     LVHQLQRPVWVLRQEIGAKPNIFYMKG"
     gene            4777..5529
                     /locus_tag="PLF77_07215"
     CDS             4777..5529
                     /locus_tag="PLF77_07215"
                     /inference="COORDINATES: protein motif:HMM:TIGR04257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPC08679.1"
                     /translation="MKRNLVLIILLALFCIANVGSTGKSSFSDGGGILYTQPVKSVIF
                     RHQHHVDVKKISCEKCHSGLFEMQALLAQEKKDFIMDSLYKGRYCGACHNGKDAFASD
                     KQCARCHVRISEKDVGHVKGKAKPYKAPVYSTVATLGKDQRKVPFNHEKHASLNCMDC
                     HSKLFQIRKGSTKIALETHQSQKYCFNCHNGKKEFSWNNCTKCHQNWKDIKPYGILTA
                     RADRGSCVACHTNPDEMKSLVKPPEIGGEAEG"
     gene            5620..6588
                     /locus_tag="PLF77_07220"
     CDS             5620..6588
                     /locus_tag="PLF77_07220"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HPC08680.1"
                     /translation="MDCIACHQGNKLAKTKEKAHQGRIARPSDNLKTCGICHITAGQN
                     YAKALHYTSLGQREGVKHRFSAAELKTFDQKVFEKSCRSCHASCGSCHVKSPPVGGIT
                     TGLLQGHKFVKKNEGKTCASCHGGRVYPEFTGEYGGTPDVHYQKGMMCNDCHNKQEMH
                     GDGKLYKSKNDVKDRPKCLSCHQDKGNQVAHTIHKDKVSCQGCHSSGQYRQCYSCHAV
                     HGSKAEPGFILGLNPRDRKTLTTLRVIPTIRSTFEPNGIKMENFDALPNYWNSPVHNI
                     KKRTNRTRSCDACHVNKEGFLKPEGLIKDGSKENEKLIYDIKPISK"
     gene            6605..7531
                     /locus_tag="PLF77_07225"
     CDS             6605..7531
                     /locus_tag="PLF77_07225"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPC08681.1"
                     /translation="MKRMRFLKVLLVSLLILLPAAVLAQGLSPIVSTDDLAKIVKDPN
                     VIVIDIRKVEDYKAGHIPGAIGVFYNNWAPGMGGLKNEMPADDDLMDLLTDNGIQPDS
                     TVVVYGTTGTPPDRVDVTRVAWALKYAGVQKVSVLSGGFEKWAVREKKEVSTEITKPK
                     TKDYKGKINKAILVNKDYVKNHVGKAMIVDAREPAFFKGEQKLPFVAKAGRVKNSVNL
                     PTIQVFDKYPPGEHMEPCCWTYKDVATLKNIASGVVGSDIDKEIIVYCDTGKVASAWW
                     FILSDVMGFKNVKIYDGSMEEWMNDASAPTEP"
     gene            complement(7610..8533)
                     /locus_tag="PLF77_07230"
     CDS             complement(7610..8533)
                     /locus_tag="PLF77_07230"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HPC08682.1"
                     /translation="MKNHALSKLLTILLFILLLPIAGQTRDIEPIVSTDWLLANLKNP
                     KLIILDVRRVEDYREGHIPGALNAFYRSWAYQKDGLFTEIPETDDLDDTIGYFGIGFD
                     SRIVVTGCMDTPRLSYQSARVACTLQYAGIKNVAVLNGGMNKWITEKKPLSQEIMHAK
                     AKPFKGLYSRARFADKDYVKERLGKAIMLDVREREFFTGEKKMDCIPMMGRIPGAFNM
                     PTSCAFNPDKTFKTTEELAAISEAAIGRDKEKEIITYCDIGQCCPIWSYILVEVLGYK
                     NVRLYDGAMQEWMQDPQAPITKNADANVTPK"
     gene            complement(8667..9212)
                     /locus_tag="PLF77_07235"
     CDS             complement(8667..9212)
                     /locus_tag="PLF77_07235"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(8994..8996),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPC08683.1"
                     /translation="MRITKFVFFVLVFFILSACASTGAKNASPVSGQVAPDFTLSDQK
                     GNIWKLSNAVKNHRAVVLAFYPKDDTKLUIRQFVEFQQNIKKYKAKKIKILGLSRDSA
                     ESHRKFIAKHSLKNMTLLVDEKGKIAKLYDADHWLLPLSKRVYVIVDKKMNIIYRNDM
                     GFALLPDQTQTLMGEIDRHIR"
     gene            complement(9281..10006)
                     /gene="prxU"
                     /locus_tag="PLF77_07240"
     CDS             complement(9281..10006)
                     /gene="prxU"
                     /locus_tag="PLF77_07240"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084511687.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(9752..9754),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HPC08684.1"
                     /translation="MAEEIKAGCARPTGGPVGEKPDAKKETSQPTPVKEVKSMVTVGK
                     KAPDFTAPAYHKGKFINLKLSDYLGKWVVLCFYPGDFTFVUATEISSVGEKYSEFKKL
                     GVEVLSMSVDSVFVHKMWDDHELSKMVKGGIPFPMLSDGGGKVGDIFGVYLQDAGVEA
                     RGRFIIDPDGIIQGFEVLTPPVGRNVMESIRQIQAFQHVRKTKGAEATPSGWKPGKPT
                     LKPGPDLVGKVWEVWKTKMAFDD"
     gene            complement(10027..10341)
                     /locus_tag="PLF77_07245"
     CDS             complement(10027..10341)
                     /locus_tag="PLF77_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPC08685.1"
                     /translation="MPEFGTPFSGLKKDRKLTKEELIRAIRFMVAAEYEAIQLYMQLA
                     ESTDNKLAIEVLGDIADEERVHAGEFLRLLHELAPDEGKFYAEGAEEVEEEIAKMKKS
                     KK"
     gene            complement(10451..12151)
                     /locus_tag="PLF77_07250"
     CDS             complement(10451..12151)
                     /locus_tag="PLF77_07250"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPC08686.1"
                     /translation="MAQRKRIIVIGGSAAGPKAAAKARRIDNDAEVIIIQKEADLSMA
                     SCGYPYYVGGFFNDRNALLCTPAGVVRNPMFYLNAKDIEAKVNTEVTNIDTKNRSVSF
                     KNTVSGETGTLTYDKLVIATGAVPRMPPVPGTDLQGITTLQSMKDADFLRNVRDEGKI
                     KKAVVIGGGLIGIETCEALQLAGIEITVIELLPQLLTFLDWELAKLVENHVRSKGANI
                     ITENGIAAFLGENGKLTAVKLQNGTELPCELAVIAIGVRPNVTLAKEAGLKIGELGGV
                     EVNEYMQTSDPDIYAVGDCVETLNRITGKKVHAPYGDLANLQGRIAGENAASGNSVTF
                     PGTIQTGICKVFDYGAGSTGLSETMAKRLGYSDIITVINASPDKPGFMEGKLLVTKLV
                     AEQKTGRILGAQCIGPGNVAKQIAQWAMAIQGKMTVEDLINADLPYAPPFSLAIDHFI
                     ATAHIMQNKMKGRMKGISCKEVYDKIRAGEKPFLIDTRGPEEFEEMRLGIGETLIPLG
                     AIRKRLKELPEDKNREIILYCKISLRGYEAALALEGNGWKNVKVMEGGIMAWPFPREK
                     "
     gene            complement(12401..13594)
                     /locus_tag="PLF77_07255"
     CDS             complement(12401..13594)
                     /locus_tag="PLF77_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPC08687.1"
                     /translation="MSYAKLSDWNKCTTCGECLVQCPVMHMDKPEAVAEMTGLLKTGY
                     SEKIFRECTFCFSCNNYCPEGLRLHEFILEKFMENRKKKGKISAVYPYLFNGMKMSMW
                     KDIYAMLSREEQSILKKWSEIPPPSKEILWVGCIGRISCFDIENSAVLQKLPKFGPPD
                     LCCGELAYRLGSWKAYTDTIEKTLKAFEKLNIERMVCYCGSCYNYLSNILKNVYGREL
                     PFKLISLYEWLNEKRESGKITVKQPLKLKTTLHESCYVSELGDGFARDLRAVYKSAGA
                     DIVELPHHGECNLSCGAVSVLRTLNLPKSLFKEQWKKYKEVKETGMKDMAVNCPGCFI
                     TLSFTNRIAGIKLHYMPDELLKAYGDTITKPLSRRIPLFIRAFIKHPTLPLRHVDAEI
                     SPASS"
     gene            complement(13613..>14205)
                     /locus_tag="PLF77_07260"
     CDS             complement(13613..>14205)
                     /locus_tag="PLF77_07260"
                     /inference="COORDINATES: protein motif:HMM:NF023646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HPC08688.1"
                     /translation="LLKNHKQIKLDKVILAACPEEIMRQALRANKKALEKCDALIVLS
                     CAAGIKSANSCRPGLPVINVLDSVGSAAVSSSDPVIAHSLCRSCGHCVLTCTAGICPL
                     SECPAKSKYGPCKSYSESNLKCALDPQINCVWHIIHERSNNLHLLPLLKELHSHKEDR
                     NTHSTHEIKSAGLWQKFFAWHAARIQKLEWVARQFR"
BASE COUNT         3591 a         3448 c         3389 g         3777 t
ORIGIN      
        1 acgaaggcac ggtgaccacg aaatatgaga acaatatcgc ccacctcaaa atcacaggtc
       61 tcaatttcag acataattat ttcgaatacc tgatcatggg attttttcag aaatcgctca
      121 aaatgttcgg gaagaaaaac gttcccaaac ggatcagaag ccgcgcatcg ggcgatgacg
      181 atgtttattt ccagtttgaa ttaaaagatt cttaacgggt tgttttgaaa cttcgatcaa
      241 cgcaaaaagc ggtgcatccc gcattgtaga gtatccatca tttgccgttc tgatcggacc
      301 gcgtgaaatt acaagcggtc tgaccggaaa cccgagggac gttttttgat gctgttgatg
      361 tgtcgtataa agcaaacaca acaaattgct ctttagatat agttgtgcag gctggttaga
      421 aaattcacac ccagataagt aaataaaaca gacacgaacc cgacaatcgt catcatggcc
      481 atgcgccggc cgcgccagcc gcggacaaag cgggcatgca gcataatggc gtagataagc
      541 cacgtgatca gggaccatat ctctttggga tcccagctcc agtatgagcc ccaggcatag
      601 tgcgcccaga cggaaccggt gataatcccc atggccagaa aaataaatcc gagtgatgtg
      661 ctctgataga tcagaacatc cagtgtgttc agcacggaag aattttcctt cggatgagac
      721 gcagggttgg tttctcttgt ttcttttttt tccgtccaga gataaaggag tccgcagccg
      781 aaagcaaccg tgaaggcggc ataacccata aagcaggtta aaacatgagt cgtcagccag
      841 ttgctttgca gcgcgggaat cagcggttcg atgctgtgat cgacccccgg tgcgatggaa
      901 gcgtaagcca tgacaaaaaa agccaccggg acgacaaacg tgccgagaat tctgcttttg
      961 atccgccact caatggccag atagagcatt atgatggacc aggcgaagaa aaccagagac
     1021 tcatacaaat tggtaatcgg cacgtgtccg aatcccaggt cgtaggaagc tctccaccgc
     1081 aacatcatgg ccagcgtgtg cgcgaaaagt cccagccacg ccagcaagga tgccgcgtat
     1141 ccccagaatt cttttttcaa aatcattctg aacagataaa aaacaaagga agcaaaataa
     1201 ataaacgtca cgtaacttaa aatcagcgtg tggttcatgc cgccagctcc ttttccatcc
     1261 atgcgcacaa atcatcaagc cgcctctgca acgcttcgtt ataacgatga ctgcgcccgg
     1321 ccacgcggat tctgacgcct gccggatcct gttccagcag aaaccagata cgccgatacg
     1381 caatgaaaaa gatgaccatc atgccggcga tcagaatcaa tccgccggcc agaacaaacg
     1441 ggacacccgg atcgcggaca acctgtaacc cggtgtaata ttttccttca atcccccgga
     1501 gagaaaaaac caggggtttg aaaaggcccg gattaaaaag agggacggcg gaaaataagc
     1561 cggggtttac atccgcgata tcctgaatat gctgaaacac ccagaactgg atatttctct
     1621 tgtccgtttc aatatttagt tttacggccg gaccgaactg catcatattt tcttccacgc
     1681 gcaaaacggt ggcctttgcc ttctgaacgg gaagatcaaa cgtgtctccc tgtcctgctg
     1741 agatttccgg gctctccgct ccggcgttgc tgtatgtcag acgggctctg cttttttcag
     1801 agacaccgta gcttgcctga taaaaacgca ggccgtcaaa cgatagcgga tgattgacca
     1861 gcacagaacc ctgacgcacg acctgtccat ttttgatgaa acttaactcc gatcgatagg
     1921 tttttgggac tcctgtgtca taaaattcca caacaaattt gtcgcatcga acggaaaaat
     1981 caagcgggtg ttcaccctcg ccattccgca gctggataat gtttgtttct tcgccttcgg
     2041 aaagtctgag atccgcctca agaccgaaca ccgagccgat caccatgccc gcaataatca
     2101 tcaaaacgcc aagatggaca acgtaaacgc cgaacaggga taggcgcccc gattcgcgat
     2161 aaagcatttt ccccttttcc agctttgttt ctttaaccga aaatagtttc gatttgagaa
     2221 gagattcgac cgcgcggctg acggtttcca tctttttatc cgtcacgatt atccggtttg
     2281 gagcaagatt ttcaaacagc ccggaaggtg gcgggaaaga gggcgtcttg tattgtttta
     2341 tggcgaaagg aattcgttgg atggagcaaa ccatcagatt cagtgagaaa agccccatca
     2401 acacataaaa cattttggaa tgataaaggt cattgagcca gagccattcg gaatccattc
     2461 tctgcggcgg caccagcgtg gccacaataa agatcaccac catgaaaaaa agcaaaacga
     2521 tggcaagttt gacggaagca agaaaagacc agatgaaatt cgcttttttc aatggtttct
     2581 gcccttaaag aaaagaaaat ttttccagaa aacttaacat ctaatgtgct ttctgtcaaa
     2641 gagtaccttg ctcagacagc ccggacgaac ttcttgacaa aacaaccggt ttaccttatc
     2701 gtttaattgt ctttcagtgt tttattgatg tgattgtcgc agcaattcaa caaggaaaat
     2761 gatatggccg attccggcaa aaagcggttc ctcccacata tcaaatctgc ctttgaacgc
     2821 gtcttatccg gtggatggat ttatctcttg tttgtcggcc tgttcggtat ttttcttctc
     2881 ataggaattg gtgccgggat tcatgcgttg tttatcgccg gcagccgtca tgcctacgcg
     2941 acatcacggg aaattcccgt tgccattctg attgccacgt atgttttttt tgttgtcgct
     3001 tcaacggggc tttgtctggt gtcgtcactg ggacatgtct tcggcgtgga aaacttcctg
     3061 cccctggcga aacgttgtgt ttacctggcg atcattacca ttctttccgg attcctggtg
     3121 atcggcctgg aaattgaaaa tcctttccgc atggcgatat acaacgttat ttcgcccaac
     3181 ttctcttcca atatctggtg gatgggaacc ctgtacgggt ttgccgtcgt ttttctgacg
     3241 attgaattca ttcttctgtt taatgaagcg caccaccata aagctgtcat tgccggtttc
     3301 ctcggcgtca ttacggaagt cgccgccaac agcaacctgg gcggtgtttt cgcgatgatg
     3361 catgggcgcc cttactggta cggcccttat ttccccgttt attttatttt atccgccctt
     3421 attacgggct gcgcttttat tctgtttttc ttttttctcg cccacaaagc ggtcaacaaa
     3481 aaaccattca tggaatcgac gcaaaccgct ctgaaagccg tatcgaaact gacgctgctt
     3541 ttgctggccg tcatgatgat cttcaccgcg tggaaggttt tgacgacctc cgttggcagt
     3601 cctgccgccg ccgccgccgc gttgagcgtg ttaactgaag gaccctattc attcaacttc
     3661 tggaccattg aaatccttgt cggaatggtc attccttttg ttttaattct ggcatcaaga
     3721 ggcaggaatc tgacggtcat gttcatcgcc gccgtcatga tgattttcag ccttttcttc
     3781 acccgtctgg atatggtggt tgtcggacag attgtcccgc aatattttga agtgggtgtt
     3841 gtggaatatt ccaaattgca tacctattct ccgagcattc acgaaatcct tgtggtgctg
     3901 ggagggataa gtttctgcct gctggcattt ttgctcggtg aaaaagtatt ttccggtttc
     3961 gcagattcac cgcagaccga catcatgaaa gaaaaaacaa cggacgttgc cggagtggaa
     4021 gaactgacat gacggataag gcgagccata aaaatattcc ggaatctccg cttgatgctg
     4081 aacggcgggc atttctgaaa atcggtctga aaattaccgg cgtgctcgcc ggcggctcaa
     4141 tattgtcttt agtctccaac atcaacaagg cttattctct tgatcttttt cgggagaaat
     4201 acccttataa acctcattac gcgatggtgt taagacagga ccgctgcatt gattgcgaaa
     4261 aatgcatgaa agcctgtatt gaaaccaatc atgttccgga ttacggctgg cggacaaaga
     4321 ttttaaaacg cgacctttcg tcggcgcttg atcagaaatg ggaatttatt cccatcctct
     4381 gcaatcaatg caacaatcct ccgtgcgtgc gcacctgccc gacgacggcg acgtataagg
     4441 ataagaccaa cggcatcgtg cagatgaaca acaaaaaatg catcgggtgc aaatcctgta
     4501 ttatggcctg cccctacgat gcccgctatt ataatgaaga aaaaggtgcc attgataaat
     4561 gcgatttctg ttttgagtcc aggctgtcca aaggcattaa taccccggcc tgcgttgaag
     4621 cctgtccctc ggatgtccgt attttcggag atttgtcgga tccgtccagc agagtgtttc
     4681 aacttgttca ccagttgcaa agacctgtgt gggtgctgcg gcaggagatc ggtgcgaaac
     4741 cgaatatttt ttatatgaag ggttgatgat tcagcaatga aaagaaatct tgttcttata
     4801 attcttctcg ctcttttctg catcgccaat gtcggttcta ccggaaaaag cagtttttcc
     4861 gacggcggtg gcattctgta tacgcagccg gtgaaatccg ttattttccg ccatcagcat
     4921 catgtggatg taaaaaaaat aagttgtgag aaatgccaca gcggcttgtt tgaaatgcag
     4981 gctttgcttg cccaggagaa aaaggatttt attatggatt ctctctacaa gggcaggtac
     5041 tgcggcgcgt gtcataacgg caaagacgct tttgcatccg ataaacagtg cgccagatgc
     5101 catgtccgca tcagcgaaaa agatgtgggg cacgtgaaag gtaaagccaa gccatacaaa
     5161 gcgcctgttt acagtacggt ggccactttg ggaaaagacc agcggaaggt cccgttcaac
     5221 cacgaaaagc atgcgtcgct gaattgcatg gattgtcatt cgaaactttt tcaaatcaga
     5281 aaaggatcaa ccaagattgc cctggaaacg catcagtcgc aaaaatattg ctttaactgt
     5341 cacaacggga aaaaagagtt ttcctggaac aattgtacaa aatgccatca gaactggaag
     5401 gatattaaac cgtacggtat tctgacggca agagccgaca ggggaagctg tgttgcctgc
     5461 cacaccaatc cggatgaaat gaaatcgctc gtcaagccgc ccgaaatagg cggcgaggcc
     5521 gaggggtgag ctggcgctcc tcccccggtc cgggcggaag aatactacaa aggttatctg
     5581 gtcgataaag gaatgatcgg caaggagttc catctttcga tggattgcat tgcctgtcat
     5641 caaggcaaca agctcgcaaa aacaaaggag aaagctcatc aaggacgtat cgccagaccg
     5701 tccgacaatc ttaaaacctg cggcatctgt catataacag ccggacaaaa ttacgccaag
     5761 gcccttcatt atacgagcct gggccagcgc gaaggcgtca aacaccggtt ttctgcggca
     5821 gaattgaaaa cgtttgatca gaaagttttt gaaaaatcat gccgatcctg tcacgcttcc
     5881 tgcggaagct gccacgtcaa atcgccgcct gtcggcggca tcaccaccgg ccttctccag
     5941 ggccataaat ttgtgaagaa aaacgaaggc aaaacctgtg cttcatgtca cggtggaaga
     6001 gtatatcccg aatttaccgg tgaatacggc ggcacccctg atgtccacta ccagaagggc
     6061 atgatgtgca atgactgcca caacaaacag gaaatgcacg gcgacggcaa attatataaa
     6121 tcaaaaaatg atgtcaaaga ccgcccgaaa tgcctgtcct gccatcagga taaaggcaat
     6181 caggtcgcgc acaccattca taaggataaa gtcagctgtc agggctgcca ttcttccggc
     6241 caatatcgcc agtgttatag ctgtcatgcc gtgcatgggt caaaggccga gcccggcttt
     6301 atcctggggc tgaatccgcg tgaccgcaag accttgacaa ccctcagggt catacctact
     6361 ataagaagta catttgagcc taacggaata aaaatggaga attttgatgc tctgcccaat
     6421 tactggaatt cgccggtgca caacattaaa aagcgaacaa acaggacacg ctcctgcgat
     6481 gcatgccatg tgaataaaga aggtttcctg aaacctgaag ggctgattaa agacggatct
     6541 aaagaaaatg agaaactaat ttatgacatt aaaccaataa gcaaataaaa acaggaggat
     6601 ctttatgaaa agaatgaggt ttttgaaagt gttgttggta tccctgttga ttttactgcc
     6661 ggcagccgtg ctggcgcagg gactcagccc tattgtttcg acagatgatt tagccaaaat
     6721 tgtaaaggat cccaacgtca ttgtcattga tatccgcaag gttgaagatt ataaagcggg
     6781 gcatattccc ggcgcgatag gggtgtttta taataattgg gcaccgggca tgggcggctt
     6841 aaaaaatgaa atgccggcag atgatgattt aatggacctt cttaccgata acggcattca
     6901 acccgactca acggtcgtcg tttacggcac tacaggaact ccgcctgatc gtgtggatgt
     6961 gacccgcgtg gcctgggctt tgaaatacgc aggcgtccag aaggtatctg tactgagcgg
     7021 cggatttgag aaatgggcgg tgcgcgagaa aaaagaagtt tctacagaga ttaccaagcc
     7081 caagaccaaa gattacaaag gtaaaatcaa taaagccatt ctggttaaca aggattacgt
     7141 gaaaaatcac gtcggaaaag cgatgatcgt tgacgcacgg gaaccggcat tctttaaagg
     7201 cgaacagaaa cttcctttcg tggccaaagc cggtcgagtc aagaattcgg tcaatctgcc
     7261 caccattcag gttttcgaca aatatccccc cggcgagcat atggagccct gctgctggac
     7321 atataaagat gtcgccacgc tcaaaaacat tgcttcaggt gtcgttggca gtgacatcga
     7381 caaggaaatc attgtctatt gcgacaccgg gaaagttgcc agtgcctggt ggttcatttt
     7441 aagtgatgtg atgggtttca aaaatgtgaa aatttatgac ggttccatgg aagaatggat
     7501 gaatgatgca agcgctccga cggaaccgta aagcataaag aacatcatga aaaaaagcag
     7561 ccccatcgtt ttatggggct gcttttctat tgaacgttaa acagactttc tatttaggcg
     7621 tgacgttggc gtcggcgttt tttgtaatcg gtgcctgagg atcctgcatc cattcctgca
     7681 tggcgccatc gtatagacgg acgtttttat agccgaggac ttccaccaga atataagacc
     7741 agatcgggca gcactgaccg atgtcgcaat aggttataat ttctttttcc ttatccctgc
     7801 cgatagcggc ttcggaaatc gccgcgagtt cttccgtggt tttaaatgtt ttgtcaggat
     7861 taaaggcgca ggaagtcggc atgttaaaag ctcccgggat tcgtcccatc atgggaatac
     7921 aatccatctt tttctcgccg gtaaagaact ctcgttctct cacatccagc ataatagcct
     7981 tgcccagccg ttctttaacg taatctttgt cggcaaatct cgctcttgag tagaggcctt
     8041 tgaatggttt tgcttttgcg tgcatgatct cctgcgaaag cggtttcttt tcagtaatcc
     8101 atttattcat cccgccgtta agaacggcta cattttttat tcctgcgtat tgcagggtgc
     8161 aggcgacacg ggcgctttga taactcagcc gcggcgtatc catgcaaccg gtgacgacaa
     8221 tccgcgagtc aaacccgatt ccgaagtagc ctatggtatc atccaggtca tccgtttcgg
     8281 ggatctccgt aaaaagaccg tctttctggt atgcccatga tctgtaaaac gcgtttagcg
     8341 cgccgggaat gtgtccctct cggtaatcct caactcggcg aacatccagg ataatcaatt
     8401 tcggattctt caaatttgcc aaaagccagt cggtggatac aatcggctcg atgtcacggg
     8461 tttgaccggc tatcggcaat aaaaggataa aaagaaggat cgtaaggagc ttggacaaag
     8521 catgattttt catatggcaa tctcctttga atgttgtatt acacatctta aaacggatgt
     8581 atacatagta taccactaat tctttattga gacagccgaa tatttcagac cgcagaatac
     8641 aagccctatt tcatactatt tttatcttac cgaatatgac ggtctatttc ccccatcagc
     8701 gtctgcgtct gatcgggcaa cagcgcgaat cccatgtcat tcctgtaaat gatgttcatt
     8761 tttttatcga caatcacata cacgcgtttg gaaaggggca aaagccagtg gtcggcgtcg
     8821 tagagcttgg cgattttgcc tttttcatcc accagcagag tcatgttttt caggctgtgc
     8881 ttggctataa acttgcggtg cgattcggcc gaatcccgcg acaggccgag aattttgatt
     8941 tttttggctt tgtatttttt aatattttgt tgaaactcaa caaactggcg gattcaaagc
     9001 ttggtgtcgt ctttgggata gaaagccagt accacggcgc gatgattttt gacagcattg
     9061 ctgagtttcc atatgttgcc cttttgatcg gacaatgtaa aatcaggggc tacctggccg
     9121 gaaaccgggg aggcgttttt tgcccctgtt gaagcacagg ctgacaatat aaagaacacc
     9181 agcacaaaaa aaacgaattt tgttattcgc atatagccct ccgataaagg tatggacggc
     9241 gggcggttca aaaataaccg cccgcccatc ttagtaaaga ttaatcgtca aacgccattt
     9301 tggttttcca aacttcccag accttgccta ccagatcggg gccgggtttg agcgtcggct
     9361 tgccgggttt ccaaccggag ggcgtggctt cggcgccttt ggttttgcgc acgtgttgga
     9421 aggcctgaat ttgacggatg ctttccatga cgttgcgtcc gaccggcggc gtcaacactt
     9481 caaagccctg aatgatgcca tcggggtcga tgatgaaccg gccgcgggct tccacgccgg
     9541 catcctgaag gtaaacgcca aagatgtctc cgaccttgcc gccgccgtcg gaaagcatgg
     9601 ggaaggggat gccgcctttg accattttgg aaagttcatg gtcatcccac attttgtgga
     9661 caaagacgct gtccacactc atggataaaa cttcaacgcc gagttttttg aattcggaat
     9721 atttctcgcc gaccgacgag atttcggttg ctcagacgaa ggtgaagtcg cccggataaa
     9781 aacacaggac aacccatttc cccagataat cggagagctt cagattgatg aatttcccct
     9841 tgtgatacgc gggcgccgtg aagtccggcg cttttttccc tacagtgacc atgcttttga
     9901 cctccttgac aggtgttggt tgagacgttt ctttcttcgc atccggtttt tcgccgaccg
     9961 gtccgccggt gggtctggca cagccggcct tgatttcttc agccatagat gattccctcc
    10021 tttgttttat ttttttgatt tcttcatttt cgcgatttct tcctccactt cctcagcgcc
    10081 ttccgcatag aattttcctt catcgggggc cagttcatga agcaatctta aaaactctcc
    10141 ggcgtgaacg cgttcctcgt cggcaatgtc cccgaggacc tcgatggcca gcttgttgtc
    10201 ggttgattcc gcgagctgca tataaagttg gatggcttca tattctgccg ccaccataaa
    10261 gcgaatggct ctgatcagtt cttctttggt cagtttcctg tccttcttca accctgaaaa
    10321 aggtgttcca aactccggca tggtatcatc cccctttcct gttattatta taggttaatt
    10381 tcctgatgca gtaacgataa tttatgacat cattttgtga tgtcaatttc tgcccttatt
    10441 tttttgatat ttacttctcc ctcgggaacg gccaagccat aatgccgcct tccatcactt
    10501 tgacgttttt ccagccgttg ccttccagtg ccagcgccgc ctcataaccg cgcagtgaaa
    10561 tcttgcagta taagatgatt tcgcgatttt tatcctccgg cagctccttg aggcgcttac
    10621 ggatcgcgcc gagcggtatc aacgtctcac cgatgcctaa acgcatctct tcgaattcct
    10681 ccggtccgcg tgtatcaatc aggaatggtt tctctccggc ccgtatttta tcataaacct
    10741 ctttacaact gatacctttc attctgcctt tcattttatt ctgcatgatg tgcgccgtgg
    10801 cgataaaatg atcaatggcc agagagaagg gcggcgcgta gggaagatcg gcgttaatca
    10861 ggtcttccac ggtcattttc ccctgaatgg ccatggccca ctgggcaatt tgcttggcga
    10921 cgtttcccgg accgatgcat tgtgcgccga ggatacggcc ggttttttgc tccgccacca
    10981 gtttagtgac cagaagcttg ccttccataa agcccggttt gtcgggactg gcgttgatga
    11041 ccgttatgat gtcggaatag cccaggcgtt tggccattgt ttccgaaagg ccggtggagc
    11101 ccgccccgta atcgaacact ttgcagatgc cggtttgaat cgttcccggg aaagtgacgc
    11161 tgttgccgga agcggcgttt tctcccgcta ttcttccctg gagattggcc agatcgccgt
    11221 aaggcgcgtg gacttttttg ccggtgatgc ggttgagggt ttccacgcag tcgcccacgg
    11281 cgtaaatgtc cgggtcggag gtctgcatgt attcattgac ttcaacgccg ccaagctcgc
    11341 cgattttcaa cccggcttct ttggccagtg tgacgttggg acggacaccg atggctataa
    11401 cggccagctc gcagggaagc tccgttccgt tttgcagttt caccgctgtg agttttccgt
    11461 tttcacccag gaaagcggct atgccgtttt cggtgatgat gttggcgcct ttgctccgca
    11521 catggttttc caccagcttg gccagttccc agtccaaaaa cgtcagcagt tgaggaagca
    11581 gttcaatgac ggtgatttca atacccgcca gttgcagcgc ttcgcaggtt tcgataccga
    11641 tgagtccgcc gccgatgacc acggcctttt tgattttgcc ttcatcgcgg acgttacgca
    11701 gaaaatcggc gtccttcatg gattgtaatg tggtaatgcc ctggagatct gtgcccggaa
    11761 ccggcggcat tcggggaacg gcgccggtgg cgataacgag tttgtcatac gtcagggtgc
    11821 cggtttctcc ggaaaccgta tttttgaagg aaacggaacg gtttttcgtg tcgatgttag
    11881 tgacttcggt gttgaccttc gcttcgatat ctttggcgtt gaggtaaaac atcggattgc
    11941 gcacgacgcc cgcgggcgtg caaagtagcg cgttgcggtc gttaaaaaaa cctccgacgt
    12001 aatacgggta gccgcaggac gccatggaaa ggtcggcttc cttctggata atgatcactt
    12061 ccgcgtcgtt gtcaatgcgc cgggctttcg ccgctgcttt gggaccggca gccgaacctc
    12121 cgataacaat gattcgtttt ctctgagcca tagttttatc ctccgatttt taaaagtgac
    12181 ctgaacataa aaggtcgctt ccataaattt gattctctca gtgaaaaaat agcaattatc
    12241 ggcagccgtt gcattgactt aaatcaatgc ggataattat tttcagagtc cgtatatttt
    12301 gagccggccg gcatcagacc ttatggaaca tacatggcaa cgacctcatc cgcagcataa
    12361 attaccccgc cggcgattag ctgtgattta ataataactt ttacgatgat gcgggcgata
    12421 tttcagcatc aacgtgcctc aacggaagcg tgggatgttt tatgaaagcc ctgatgaaaa
    12481 ggggaatcct tcgggataac ggtttggtga tcgtatcccc gtaggctttc agcaactcat
    12541 ccggcatgta gtgaagcttt attcccgcaa ttctgttggt gaaggagagc gtgataaaac
    12601 atccggggca gttgacggcc atatctttca ttccggtttc cttcacttct ttgtattttt
    12661 tccactgttc cttgaagaga cttttcggaa gattaagggt ccggagaacg ctcacggccc
    12721 cgcaggaaag gttgcactct ccatgatggg ggagttccac tatatcggcg cccgctgatt
    12781 tgtaaacggc gcgaaggtcg cgggcaaagc cgtcacccag ttcgctgaca taacatgatt
    12841 catggagggt cgtctttagt ttcaacggtt gttttactgt aatcttgccg gattctcttt
    12901 tttcattcag ccattcatac agggaaatca atttgaatgg caattccctg ccgtatacat
    12961 ttttcagtat attggataga tagttgtagc aggatccgca gtagcaaacc attctctcga
    13021 tattcaattt ctcaaaagct ttcagggttt tttcaatcgt atcggtgtag gctttccatg
    13081 aaccgagacg gtatgcgagt tcaccacaac agaggtccgg cggcccgaat ttcggcagtt
    13141 tctgtaaaac agcggagttt tcgatatcaa aacaactgat tctgccgatg catcccaccc
    13201 acaaaatttc cttggatggc ggtggaattt ccgaccattt tttcaatatg gattgttcct
    13261 ccctggaaag catggcataa atatccttcc acattgacat tttcatgccg ttgaagagat
    13321 aggggtagac agcggatatc tttcctttct tcttcctgtt ctccatgaac ttctccagaa
    13381 taaattcatg aagtcttaac ccctcggggc aataattatt gcaactgaag cagaacgtgc
    13441 attccctgaa aattttttcc gagtaaccgg ttttcaataa tccggtcatt tcagcaacgg
    13501 cctcgggctt gtccatgtgc atcacgggac attggacaag acattcaccg cacgtcgtac
    13561 atttattcca atccgataat ttggcgtatg acataataca tgttccccct ttttatctaa
    13621 attgacgagc aacccattca agtttctgga tacgcgccgc atgccatgca aaaaattttt
    13681 gccagagccc ggcgcttttt atctcatgcg tcgaatgcgt gtttcgatct tctttgtgtg
    13741 agtgtaattc ttttaataac ggcagtaaat gcagattatt gctccgttca tgaatgatat
    13801 gccatacgca atttatttgc ggatcgagcg cgcatttcaa gtttgattct gaataggatt
    13861 tgcaggggcc gtatttgctt tttgcgggac attcgctcaa cggacagatt ccggcggtgc
    13921 aggttagcac gcaatgtccg cagcttctgc agaggctatg tgcaataacc ggatcgctgc
    13981 tagaaactgc ggcgcttccc acggaatcca gcacattgat tacaggcaaa cccggccgac
    14041 agctgttcgc tgattttatt cccgccgcgc aactcaaaac gatcaatgca tcacactttt
    14101 cgagggcctt tttgttggcc ctcaacgcct gacgcattat ttcttcggga caagcggcaa
    14161 gaatgacttt gtccagtttg atctgtttgt gatttttcag cagat
//
LOCUS       DAOOLB010000050        14195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4620_length_14195_cov_40.595262, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000050 DAOOLB010000000
VERSION     DAOOLB010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14195
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4620_length_14195_cov_40.595262"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..2176
                     /gene="fusA"
                     /locus_tag="PLF77_07265"
     CDS             92..2176
                     /gene="fusA"
                     /locus_tag="PLF77_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPC08689.1"
                     /translation="MAKYETKSIRNLAVVGHGGTGKTSLCESILYVSGKSERLGRVDD
                     ATSCMDYEPEEQKRRGSISSAANFAEWQKHKINFIDTPGDSNFAYDIKCSMSVVENAV
                     VVVDAVGGVEFQTQKVWELADEFKLPRIIFINRLDRERANFNAALDSIKNKLKRKATP
                     VCLPIGSEDQFNGIVDLIGMKAYLFSDNKGIGKAVDVPADMKDDVKLLHDSMVEDIAE
                     SDDELMNKYLESGELSDEELKAGLKKGVASGSFIPVICGSAVKGIGISMLLDLCVDAF
                     ASPVDRGAIKGTKPGTDNVEERQPNEDTPFSAIVFKTIADPYAGRLTLFRVYSGKLHS
                     DSPVYNSTRKMTEKFGHIFFLEGKNQKQTEELIPGDIAGVAKLKETTTGDTLCNEKSP
                     IIFEKVPVPPPIMSFAIEPKSRGDEDKIASSIHRLTEEDPTIQFTRNVETKEMILSGM
                     GQVHIEVTIEKMKRKFGVEVNLNTPKIPYKETIKGKTNVQGKYKKQSGGRGQFGDCWI
                     DIEPLPRGGGFEFHDKIVGGVIPGQYRPAVEKGIVEAAAKGVIAGYPVVDFKINLTFG
                     SYHTVDSSEMAFKIAGSMAFQKGVMDCQPILLEPIVNIEIEVPDEYMGDVIGDLNSRR
                     GRVQGMDAKGSNQIIKGQVPLAEILKYAPDLRSMTSGRGNFTYTHSHYEEVPAFIAEK
                     IIAAYKKDKEEE"
     gene            2193..2684
                     /gene="mog"
                     /locus_tag="PLF77_07270"
     CDS             2193..2684
                     /gene="mog"
                     /locus_tag="PLF77_07270"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HPC08690.1"
                     /translation="MISAGIITVSDKGSQGQRDDLSGPAIAEMLAGAAIEIRHTIIIP
                     DEKDQIRKTIIDCSDLKQLDLILTTGGTGVSPRDVTPDATLDVIDKQIPGMAEAMRHE
                     SMRVTPHAMISRAVAGIRGKSLILNLPGSPKSVRENLAVVLPALKHAIEKIKGDDSEC
                     AVP"
     gene            complement(2650..3165)
                     /locus_tag="PLF77_07275"
     CDS             complement(2650..3165)
                     /locus_tag="PLF77_07275"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HPC08691.1"
                     /translation="MMSKHPSHNKGPAETEVRFITDASLAGLAKWLRVLGYDTIVFHQ
                     TAGRSMMRCADKENRILLTRRADMVERQFSGSLFLIKDLEVGKQLKAVIERFALKIDR
                     DKMFGICLICNEKLLPVSREKVRDLVPPYVFENCSRYNECPRCKKIYWMGTHSRNAFR
                     FMEQHIPNHLP"
     gene            complement(3140..3448)
                     /locus_tag="PLF77_07280"
     CDS             complement(3140..3448)
                     /locus_tag="PLF77_07280"
                     /inference="COORDINATES: protein motif:HMM:NF018058.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HPC08692.1"
                     /translation="MRVVYLILVILFSFLIVTFSLQNSARVQLQYYHYLNIDVPTYMI
                     IFASLLIGVIITAFWGIVERFRLNRTINRLNKLVRDLRREIQATEKPLMINDVKTPQP
                     "
     gene            complement(3450..3935)
                     /locus_tag="PLF77_07285"
     CDS             complement(3450..3935)
                     /locus_tag="PLF77_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HPC08693.1"
                     /translation="METIFAPWRMEYIRGEKVNGCVFCKCSVRCDEYILFEGKTAYVM
                     MNKYPYVNGHLMIIPQRHVGQIEDLTQEEKKEIFDLLDASVKVLKEAMNPNGFNIGIN
                     IGKAAGAGVEEHFHVHVIPRWEGDTNFMSVVTNVRVIPEALDETVASLTPLFKKYCRE
                     A"
     gene            complement(3962..4453)
                     /locus_tag="PLF77_07290"
     CDS             complement(3962..4453)
                     /locus_tag="PLF77_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPC08694.1"
                     /translation="MEGKKIKIGVISDTHLDDYDPKMQKRIAGYFDDADMILHAGDMV
                     DLNVLKMFGDKDVKAVCGNMDSSSVRKTYPDHLLFEIKGFKILLIHGWGSPWGLEEKI
                     AARFKNVHCIVYGHTHRPSNHLENNVLFFNPGSAADSSFNSSKTIGILEIDKQITGRI
                     IKI"
     gene            4667..4948
                     /locus_tag="PLF77_07295"
     CDS             4667..4948
                     /locus_tag="PLF77_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08695.1"
                     /translation="MNNLEKTLNKIAENILHFDEASLTSLWAQYKNKVENFSPSAEWE
                     KAVIIFSIINAVRAKNAIFNDNLLHKKSSAKAEKPQKKIKTKSYLTLVK"
     gene            4948..6957
                     /gene="ligA"
                     /locus_tag="PLF77_07300"
     CDS             4948..6957
                     /gene="ligA"
                     /locus_tag="PLF77_07300"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPC08696.1"
                     /translation="MDKSSDLKRIIDLRKTIEYHNRRYYQQDSPEISDAEYDRLMREL
                     QDLENRYPEAFSPSSPSQRVGAAPLSKFAPFHHPSPMFSLANAFSEGEIIEFDQRLKR
                     LAGVNDIGYIAEPKLDGLAVNLIYENGVFKQGATRGDGTTGEDVSQNLKTISSLPLFM
                     KKTSEYQLPSFIEIRGEVYIEKDPFEKLNQRRTQDGEEPFANPRNAAAGSLRQLDSRI
                     TARRPLNIFLYGIGEAKGIDFSTHWEALQILDGWGFPVNRVIEKASNIEACIHYFERI
                     AAQREKLPYEIDGVVLKVDDLSLQKILGNVSRNPRWALACKFPAAQAMTVVKDIIVGV
                     GRMGTLTPVAIMEPVNVGGVMVSRATMHNEDEVLKKDVRVGDTVVIQRAGDVIPEVVR
                     IVPEKRSSDTKQFKMPAHCPECGSEVVRYEGEVAHRCVNISCPAQLKEHIRHFASRGA
                     MDIEGLGEKVSAQLFDAKLIADPADLYFLTKEQLVDLDRQAEKSAQNLIEAIAGSKNP
                     SLDKFIYALGIRHVGERTAKLLANHYGSIKNLISTTQDELTAIHEIGPEIAESIVDFF
                     HEQKNINVLNKFFKAGINPQKKEVQTSAPLQGKSFVFTGTMENMGRNEAQTIVENLGG
                     TIHSSVTKKTTYVVAGSDPGSKLDKARSSGIQIISENEFLKLIGR"
     gene            6960..7232
                     /locus_tag="PLF77_07305"
     CDS             6960..7232
                     /locus_tag="PLF77_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPC08697.1"
                     /translation="MKQIHVHVRGRVQGVFFRAATQETARGLNLTGWVRNRSDGSVEA
                     VFEGEDDHVDIMLAWCRQGPPNARVEEVLIQEEPCSGLFLDFDIKY"
     gene            complement(7336..>10937)
                     /locus_tag="PLF77_07310"
     CDS             complement(7336..>10937)
                     /locus_tag="PLF77_07310"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HPC08698.1"
                     /translation="FLIGAYRDNETNDAHPLTLALDHIRKKGKKINSIVLGPLNLTGI
                     NMIIMNVLLCDAEKSMPLAELINRKTAGNPFFVIQFLKNIYDNRLLQLNPSTGWEWDI
                     DKIRQMQVTENVIEFMAEKISHFPKNTREILKICACIGNRFDLELLSVVSGKSIDETL
                     FDLTSAIQEDMISLHGNMYKFMHDRIQEAAYSMISEEEKSAMHYRIGGNVLKKTDEKN
                     LHEKIFYIVDQLNRGIRCVSGTDEEIMLARLNLQAAQKSKKSTAYSSASFYLKTAMGL
                     LPEYAWKSHYELTYAIYKEAIECEYLNLNFSEAEKIFHAVVNNARSNIDKANVYTLMI
                     ILYTTQGNYDAALHVGMEGMKMLGIHLPDNVSDVRVGWELLKLRLKFGRRKIEDLIDL
                     QYIPMPNTLDEAKQMAAEYLRVRPGKSLASPEINLWEMLSYAYLAIHTGTVAFYANPN
                     LFAFIAINGVYRLLDYEINFEYSPFAYIALGSIVGSSLGFYQHGYRFGVAALKTNEKI
                     ADTKNRCRIEFAFPMFIQHWKKHARKDLDYFRNAYKNGIENGDLIFSGHNVNLIGMTR
                     IMLGDNLDDILEEYGKYRDFQLGGKDPFIARNYMENTRMCLCLKGLTESRGSLNGDGF
                     DAKEQAGYYKSENNLLGTFYFSLVRLRINYLFGNYSACRDLLPEMRRIVQKKTALGNL
                     HIPEFYLYSSLTMTASYPEADPMTKARYKMSLRANQMKMLIWAKACPENFQHKYDLVA
                     AEMMKNKGRFREAQKLYHKAIEGARENGYRQEEAIACERLALLYLDHRCRDEAGFFMQ
                     KAHKGYLGWGASEKARDLEEKYPSLVPLEQKQWSTGTVTVSGPSGSMTISGATETTGS
                     TKMLDLSTAMRVSQIIASEIMLDRLLQKIMHESITNAGAQRGYLLLESDGDLTIEASQ
                     DVDKGESQVMQSLPLKDCPDICHSIVNYVYHSGEDIVLGNAVKEGLFTNDPYVIRVRL
                     KSILCTPIMSKGKISGILYMENNISENAFTPERLEVLRSFSVQAAISIENARLFELAT
                     TDGMTKLYVHRYFQLLLDQEIKRSRRQDRKFSLVMMDIDNFKSFNDTYGHQLGDRVLK
                     DVAAAARKASRSEDVAARYGGEEFVMILPDTDSKQAVILAERIRAGVAAIEIPHDHQK
                     LHVTISLGVSTYPDHSEIKEELIRAADAALYESKHRGKNCVSVFEKKNEINLQ"
     assembly_gap    10938..11037
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11038..12201)
                     /locus_tag="PLF77_07315"
     CDS             complement(<11038..12201)
                     /locus_tag="PLF77_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HPC08699.1"
                     /translation="MEKFANYILSEKIHETRNSVIFRGYKETEGLPLIIKLLKTINPT
                     SSEIARFKQEYNLVKSLDVDNIVKIFDLVDYDNQYAIIEEDFGGISLKDSLKDKELDL
                     QSLLRISSKVSGTLGLIHKNNIIHLDIKPDNILINPERDEVKVADFGISAVLTHANDE
                     LYNPDVIEGTLSYMSPEQTGRMNRGVDYRTDIYSLGVTFYEMLTGEVPFKSKDPMELI
                     HSHIARQPLPPDHLNPSIPPVLSSIILRMLSKNPEERYQNCLGIMADLDECLRQLNQK
                     GQVDDFPIATKDISIRFNIPRTLVDRDHERIELMRCFERTCKGLSEFMLVTGQPGIGK
                     SALVNEIYKPIIARKGYFIFGKYDQFRKDVPYSAIIQSFQGLIRQIISESEEKI"
     gene            complement(12314..13411)
                     /locus_tag="PLF77_07320"
     CDS             complement(12314..13411)
                     /locus_tag="PLF77_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPC08700.1"
                     /translation="MKKCSACGEVQEANAIFCTNCGQRLAEDSNVKKCPSCGTSNKPE
                     DAFCSDCGSRLEGPKAQASESSPVATQLLALNNEFLSVRESSAGQFEFSSETGARSAV
                     QHVKIKYTALAILDPLTKQITFWEKMVESSAGMTAGVFAEKTVQKGIDVGKKIHGQLL
                     FGGKYGFEYGKLKDVVKDIAAKHGWNFKLNIFKPKSKTETTGLINKIPFIKVLGPIFA
                     ILLIMAIGLIAYFTLSGDSSKTTSQAGLESHDSDEVGKAGSSIFGKSKTEADSSKQII
                     QTDRDTYRSGEKIQVHYYNAPGHSRDWICIVPAGSRNTDAGDYQYIPRRGQGVLTFRS
                     PGPGRYEARAFYRYSPGRYRITARYTFTVEG"
     gene            complement(13507..>14195)
                     /locus_tag="PLF77_07325"
     CDS             complement(13507..>14195)
                     /locus_tag="PLF77_07325"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HPC08701.1"
                     /translation="IGGYFIGKGGTRFKSDTRTVTVKGLVEKVVKADQSIWVLRFRRA
                     SEDIKDAQTKISADRDAGIAFLKKQGFKDEEISRQPTRTIDKLAREYDQSQATERYRY
                     VVTSSLVVTTSNIDLVIKALGETEELLKSGVVLDGQDEGTANPRYVITKFNELRPPLL
                     AEATKNARMTAQQFASDSGARVGKIRSANQGNIQIFGSDGNDESAPYSPTSTPVKKIR
                     VVSTFEFELI"
BASE COUNT         3731 a         3395 c         3187 g         3782 t
ORIGIN      
        1 tatcaaattt gtcttgattt tacttccctt atacgttaaa cagcccaaat tcaatttgaa
       61 ttgtcttttt aaagcattga aaggagtttt catggcaaaa tacgaaacaa aatcaatcag
      121 aaatttagct gtggtcggtc acggcggaac cggaaagaca tctctttgtg aatccatatt
      181 gtatgtttca gggaaaagtg aacgattagg gcgcgttgat gatgcaacct cctgcatgga
      241 ctatgagcct gaagaacaaa aacggcgggg ctccatcagt tccgcggcaa attttgccga
      301 atggcaaaag cacaaaatca attttattga tacgccgggt gactccaatt tcgcctatga
      361 tataaaatgc tccatgagcg ttgtggaaaa tgctgtcgtc gtggttgacg ctgtcggcgg
      421 tgttgagttc cagacacaaa aagtttggga acttgccgat gagttcaaac taccccgtat
      481 tattttcatt aacagactgg atcgggaacg cgctaatttc aacgccgcgc tcgacagcat
      541 caagaataaa ttaaagagaa aagcaacccc cgtctgtctt cccatcggtt ccgaagatca
      601 atttaacggc attgttgatt taatcggcat gaaagcttac ctgttcagcg acaataaggg
      661 catcggcaaa gccgtcgatg ttcccgcgga catgaaagac gacgtaaagc tcctgcacga
      721 ttccatggtg gaagacattg ccgaatccga tgatgaactg atgaataaat atctggaaag
      781 cggtgaactt tccgatgaag agttaaaagc cggattgaaa aaaggcgttg cgtcgggaag
      841 tttcattccg gtcatttgcg gctccgcggt taaaggcatc ggcatctcca tgcttctgga
      901 cctgtgcgtg gatgctttcg catcgcctgt cgatcgcggt gcaatcaagg gaacgaaacc
      961 gggaacggac aacgttgaag aacgccagcc caatgaagat acgcccttct cggctatcgt
     1021 tttcaagacg attgccgatc cttacgccgg gaggctgact ctcttccgtg tttattccgg
     1081 aaagcttcat tccgattcgc cggtttataa ttcaacccgg aaaatgacgg aaaagttcgg
     1141 gcatatcttt ttcctcgaag ggaaaaacca gaaacaaacg gaagaattga ttcccggcga
     1201 tatcgccggc gtggccaaat taaaggaaac aacaacaggc gataccctct gcaatgaaaa
     1261 atctcccatc attttcgaga aagtccctgt tcctccgccg atcatgtcgt ttgcgatcga
     1321 accgaaatcg cgcggcgatg aagacaagat tgcttcctcc attcaccggc tgacggaaga
     1381 agacccgacc attcagttca cccggaatgt tgaaaccaag gaaatgattc tctccggcat
     1441 gggacaggtg catattgaag tcaccatcga aaaaatgaaa agaaaattcg gcgttgaagt
     1501 caatctgaac acgcctaaga tcccctataa agaaacgatt aagggaaaaa ccaacgtaca
     1561 gggaaaatac aagaagcaat ccggcggacg cggacagttc ggcgattgct ggatcgatat
     1621 cgaacccctg ccccgcggag gcggttttga gtttcatgac aaaatagtcg gtggcgttat
     1681 tccgggacaa tatcgtccgg ccgttgaaaa aggcattgtg gaagcggcgg ccaaaggcgt
     1741 gatcgccggt tatccggtag tggattttaa aataaatctt acattcggtt cttatcacac
     1801 agtagactcg tcggaaatgg cctttaaaat cgccggttcc atggcttttc aaaagggggt
     1861 catggattgt caaccgattc ttctggaacc catcgtcaat atcgaaatcg aagttcccga
     1921 cgaatacatg ggggatgtga tcggagacct caacagccga cgcggccgcg ttcagggcat
     1981 ggatgccaaa ggttccaacc agattatcaa aggccaggtt ccgctcgctg aaattttaaa
     2041 gtacgcaccg gatctcagat ccatgaccag cggcagaggc aatttcacgt acacgcattc
     2101 tcattacgag gaagttcccg ctttcatcgc tgaaaaaatc attgccgcgt ataagaaaga
     2161 taaggaagaa gaataatcac aggaatcagc tcatgatcag tgccggaatt ataacagtca
     2221 gtgacaaagg ctctcaaggc caacgtgacg atttaagcgg ccctgccata gcagaaatgc
     2281 tggcaggcgc cgccattgaa atcaggcaca ccatcatcat tccggatgaa aaagaccaga
     2341 tcagaaaaac aatcatcgat tgttcagatc tcaaacagtt ggatttgatt ctcaccaccg
     2401 gaggcaccgg tgtcagcccc cgcgatgtga cgcccgatgc gacactggac gtcattgaca
     2461 aacaaatccc cggcatggcg gaagccatgc gccatgagag catgcgcgtt acgccccatg
     2521 ccatgatttc ccgcgccgtg gcgggaattc gcggaaaaag cctgatatta aatcttcccg
     2581 gaagtcccaa aagcgtccgt gagaatcttg cggttgtcct gccggcgctg aaacacgcca
     2641 tcgaaaaaat caagggagat gattcggaat gtgctgttcc atgaagcgga aggcgtttcg
     2701 ggaatgcgtt cccatccagt agattttttt gcaacgggga cattcattgt accgggaaca
     2761 gttttcaaag acataaggcg gaaccagatc ccgtaccttt tcccgcgaaa ccggaagcag
     2821 tttttcattg cagatcagac aaatcccgaa cattttatcc ctgtcgattt tcaaggcgaa
     2881 tcgctcgatg accgccttga gttgcttgcc gacctcaaga tctttaatta aaaaaagact
     2941 gccggagaac tgccgctcaa ccatgtccgc tcttctggtc aacagaattc tgttttcctt
     3001 gtccgcacag cgcatcatgg aacgtccggc ggtctggtga aagacaatcg tgtcataccc
     3061 cagaaccctt agccatttgg ccagaccagc cagactggca tccgtgataa accggacctc
     3121 cgtttcagct ggacctttgt tatggctggg gtgttttgac atcattgatc atcagcggtt
     3181 tttcggtggc ctgaatctcc ctgcgcaaat cgcggaccag tttatttagg cggttgattg
     3241 tgcggttgag gcgaaaacgt tccacgatgc cccaaaacgc cgtgataata acgccgatca
     3301 gaagagatgc gaagatgatc atataggtag gtacgtcgat gttgagatag tgatagtact
     3361 gcaattgaac tctggccgaa ttctgcagag aaaacgtaac aattagaaaa gaaaaaagaa
     3421 tgactaggat cagataaacg actctcattc tatgcctccc tgcaatattt tttaaaaaga
     3481 ggcgtaaggc tggcaacggt ttcatcgagt gcttccggaa tgacccggac attggtaacg
     3541 acgctcatga aatttgtatc gccctcccat cgcggaatga catgcacgtg aaaatgttct
     3601 tcgacgcctg ccccggcggc tttgccgata ttgatgccga tattgaatcc gttggggttc
     3661 attgcctctt tgagcacttt aacggaagcg tccagaagat caaagatctc ttttttctcc
     3721 tcctgcgtca aatcttcaat ttgcccgaca tgccgctggg ggatgatcat cagatgtccg
     3781 ttcacataag ggtatttgtt catcatcacg taagcggttt tgccttcaaa caaaatatac
     3841 tcgtcacatc ggacggaaca cttgcagaag acgcaaccgt tgactttttc ccctctgata
     3901 tattccatac gccaaggcgc aaaaattgtt tccatatttg aaactcgccg tttttttaaa
     3961 tttatatttt tattatccta cccgttatct gtttgtcaat ttctaatatg ccgatggttt
     4021 ttgaagaatt gaaagagctg tccgccgccg aaccgggatt aaagaaaagt acgttgtttt
     4081 ctagatgatt ggacggcctg tgcgtgtgcc cgtaaacgat acaatggaca ttcttgaacc
     4141 gggcggctat cttttcttcc agcccccagg gtgatcccca gccatgaatc agtaaaattt
     4201 taaatccctt aatttcaaaa agcaaatgat ccggatatgt tttcctcacg gaagaactat
     4261 ccatgttacc gcagacggct ttgacgtcct tatctccaaa catctttaaa acattcaaat
     4321 ccaccatatc gccggcatgc aaaatcatgt ccgcatcgtc aaaatagccg gcaatacgtt
     4381 tttgcatctt cggatcataa tcatcaagat gcgtatcgga tatgactcct atttttattt
     4441 ttttgccttc cataacgtaa aaaccatggc gtcatttaac tgcttgcggc aaaaaaaaca
     4501 agctttaaaa ttagtgaaac acgccaagaa tgatattcaa tattcaggaa tgaagcgaac
     4561 tgaaacactt tggttgaatt atcctgtgtg ctatccaaat atgatttcat taaaggaata
     4621 aagctttaat ttgacattac cggcaagtaa agttaatatt tcgatcatga acaatctgga
     4681 aaaaactctg aacaaaatag ctgaaaatat cctgcatttt gacgaggctt cgctgacctc
     4741 gctctgggct caatataaaa ataaggtcga aaacttttcg ccttccgccg aatgggaaaa
     4801 agcggttatc atcttctcga ttatcaatgc cgtgagagct aaaaacgcaa tttttaacga
     4861 taatttattg cataaaaaat cttctgcaaa agcggaaaaa ccgcaaaaaa agataaaaac
     4921 aaaatcttat ctgacacttg ttaaataatg gataaatcat cggatttaaa aagaattatc
     4981 gatctgagaa aaacgatcga ataccataac aggcgttatt atcaacagga ttcgcctgaa
     5041 atttccgatg ccgaatacga tcggttgatg cgtgagcttc aggacctgga aaatcgttac
     5101 cctgaggcat tttcaccgtc ttctccctct caaagagtcg gcgcagcccc tttgagtaaa
     5161 tttgccccct tccatcatcc atcgccgatg ttcagtctgg ccaacgcttt ttccgaaggc
     5221 gaaattatcg aatttgacca gcgccttaaa agactggccg gagttaacga tataggctat
     5281 atcgccgaac cgaagctgga tggcctcgcg gttaatttga tctacgaaaa cggcgttttc
     5341 aagcagggag cgacacgcgg tgacggtacg accggcgaag atgtttcgca aaatctcaaa
     5401 acgatctctt ccctgccctt gtttatgaaa aaaacatcgg aatatcagct tccttctttc
     5461 attgaaattc gcggcgaagt ttatattgaa aaagacccct ttgaaaaact caatcagcgc
     5521 cgtacccaag acggtgagga accctttgcc aatccccgca atgcggccgc cggatcgctg
     5581 cgtcagcttg attcaagaat tacggcacgc cgccccctca atatcttcct gtatggcatc
     5641 ggcgaggcaa agggcattga tttttcaacg cactgggaag cactgcaaat ccttgacggc
     5701 tgggggttcc ctgtgaatcg cgtgattgaa aaagcatcga atatcgaagc ctgcattcat
     5761 tattttgagc gcattgccgc tcaacgggaa aaactgccct atgagatcga cggcgttgtt
     5821 ctcaaggttg atgacctatc tttacaaaag atactcggca atgtctcgcg caatccgcgc
     5881 tgggcgctcg cctgcaaatt tccggcggca caggctatga cggtggtcaa agacatcatc
     5941 gtcggtgtcg gacgcatggg aactctgaca cccgtcgcca ttatggagcc ggtcaatgtc
     6001 ggcggtgtca tggtcagccg cgccactatg cataatgaag atgaggtcct taaaaaagac
     6061 gtgcgcgtcg gagataccgt ggttattcaa cgcgccggag atgtcattcc cgaagttgtc
     6121 cggattgttc cggagaaaag atcctccgat acgaaacaat ttaagatgcc cgcccactgc
     6181 cccgaatgcg gctccgaagt ggtccggtat gaaggagaag tggcccaccg ttgcgtgaac
     6241 atatcctgcc cggcccagtt aaaagaacat atccgccatt tcgcgtctcg cggcgcgatg
     6301 gatattgaag ggctcgggga aaaagtttct gcgcagcttt ttgacgccaa gctgattgcc
     6361 gatcctgcgg atctttattt tctgactaaa gaacagctcg tcgatcttga ccggcaggcg
     6421 gaaaaatcag ctcaaaattt aatagaagcc atcgccggat cgaaaaatcc ttctctggat
     6481 aaattcatct atgcattggg tatccgccac gtcggagaac gcaccgccaa gcttctggca
     6541 aatcattacg gcagcataaa gaatcttatt tcgacaaccc aggacgaatt aacggccatc
     6601 catgaaatcg gcccggaaat agccgaaagc atcgttgatt tttttcatga acaaaaaaat
     6661 atcaatgtcc tgaataaatt tttcaaggcc ggcataaatc cgcagaaaaa agaagttcag
     6721 acgagcgcac ctttgcaggg gaaatcgttt gtttttaccg gtaccatgga gaacatgggc
     6781 agaaacgaag cccaaacgat tgttgaaaat ctgggaggca caattcattc cagtgtaacg
     6841 aagaaaacga cctatgtcgt cgccgggagc gatcccggtt caaaactgga caaggcaaga
     6901 tcttccggaa ttcaaatcat cagcgaaaat gaatttttaa aactgatagg caggtaacat
     6961 tgaaacaaat tcatgttcat gtcagaggca gagttcaggg agtttttttc cgggcggcca
     7021 cccaggaaac ggccaggggg ttgaatctca ccggctgggt gcgcaaccgg tcagacggaa
     7081 gcgtggaagc cgtctttgaa ggtgaagacg atcacgtgga cataatgctg gcgtggtgcc
     7141 gccaggggcc gccgaatgcg cgcgtggaag aagttcttat tcaggaagaa ccctgctccg
     7201 gcctgttttt ggatttcgac attaaatact gaacgcatcc ttggacaaac ttgctcccgg
     7261 cgtatcaatc acaattcgat ccggtcgtgc agaaccgcgg attgatgaca caagattttc
     7321 cttattgcgc aggagctatt gaagattgat ttcgtttttc ttctcgaaca cactgacaca
     7381 gttttttccc ctgtgctttg actcataaag ggcggcatcc gccgcgcgaa tcagttcttc
     7441 cttgatttca gaatgatcgg gatacgtcga cacgcccaga ctgatcgtga catgcaattt
     7501 ctgatggtca tgagggattt ctattgccgc aacgccggcc cggattctct cggccagaat
     7561 cacggcctgt tttgaatcgg tatccggcag aatcatgaca aactcttccc cgccgtaacg
     7621 ggccgccaca tcttcggaac gcgatgcttt ccttgccgcc gcagcaacat ctttcaaaac
     7681 cctgtcaccc agctgatgcc cgtaggtgtc gttgaaagat ttgaaattgt caatatccat
     7741 catcacgagt gaaaacttcc tgtcctgacg gcgcgaccgt tttatttcct gatcgagcag
     7801 aagctgaaaa tagcgatgga catacaactt ggtcataccg tcggtcgtgg ccagttcgaa
     7861 aagcctcgcg ttttcgatgg atatggcggc ctgaacggaa aaacttcgga gcacttccag
     7921 tcgttccggc gtaaaggcgt tttcactaat attgttttcc atgtaaagga tgccggatat
     7981 tttgcctttg ctcataatcg gcgtgcaaag aatggatttt aaacgcacgc ggataacgta
     8041 cgggtcgttg gtgaaaagac cttcctttac ggcattgccc agaacaatat cttcgccgct
     8101 gtgatagaca taattgacaa tggagtgaca gatatcgggg caatctttca ggggcaggga
     8161 ttgcatcacc tggctctcac ctttgtctac atcctgactg gcttcaatgg tcagatcgcc
     8221 atccgattcc aaaagcaaat aaccgcgttg cgctccggca ttcgtaatgg actcatgcat
     8281 gattttctgc agcagacggt cgagcatgat ttcactggca atgatctggg agactctcat
     8341 ggctgttgac agatcaagca tttttgtgct gccggtggtt tcggttgcac cgctgatggt
     8401 catcgatcct gaaggaccgc tcaccgtgac ggttcccgtg ctccattgct tctgttccag
     8461 tgggacaagc gacggatatt tttcctccag atctctggct ttctccgaag cgccccagcc
     8521 caggtagcct ttatgggctt tctgcatgaa aaatcccgct tcatccctgc atctgtgatc
     8581 aagatacaaa agcgccagac gttcgcaggc aatggcctct tcctgcctgt atccattttc
     8641 ccgggcgcct tcaatcgctt tatgatagag tttctgcgcc tcccggaagc gccctttgtt
     8701 tttcatcatc tccgccgcca caagatcata tttatgctga aaattttccg gacaagcttt
     8761 ggcccatatg agcattttca tctgattagc cctcaacgac attttatacc gggcttttgt
     8821 catcggatcc gcctcgggat aagatgccgt catagtcaat gacgaataaa gatagaattc
     8881 cgggatgtga agattcccca gcgccgtctt tttttgaaca atccttcgca tctcgggaag
     8941 aagatccctg cacgccgaat aattgccgaa cagataattg attctcaacc ggaccaacga
     9001 gaagtaaaaa gtgcccagca aattattttc agacttgtaa taaccggcct gctcctttgc
     9061 atcaaagccg tccccgttca aacttcctct gctttcggtc aggcctttca gacacaggca
     9121 catgcgcgta ttttccatgt agttgcgcgc gataaaagga tctttgccgc cgagttgaaa
     9181 atccctgtat tttccgtatt cttccagaat atcgtcgagg ttgtccccca gcatgattcg
     9241 ggtcatgccg atcagattaa cattatgacc gctgaaaata agatcgccgt tttcaatgcc
     9301 gtttttataa gcgtttctga aataatcaag gtctttgcgg gcgtgtttct tccagtgctg
     9361 gataaacatc ggaaatgcga attctatcct gcatcgattc tttgtatcgg cgattttttc
     9421 gttggttttt aatgccgcta ccccgaaccg gtatccgtgc tgatagaagc ccagagagga
     9481 tcccacaata gaccccaggg cgatataggc aaacggcgaa tattcaaaat tgatctcgta
     9541 gtccagcagc ctgtaaacac cgttgatggc gataaaagca aacagattgg gattagcgta
     9601 aaaggccaca gttccggtat gaatggccag atacgcgtac gaaagcattt cccagaggtt
     9661 gatttccgga gatgccagtg attttccggg acgcaccctc agatactccg cggccatctg
     9721 ttttgcttca tccaacgtat tgggcatggg aatgtattgc aaatcaatca aatcttctat
     9781 tttccgacga ccgaacttta agcggagttt cagcagttcc cagcccaccc ggacatcgct
     9841 gacgttatcc ggcagatgga tgccgagcat tttcattcct tccatgccga catgcagtgc
     9901 cgcatcgtag tttccctgtg ttgtatatag aatgatcatc agcgtgtaaa cattggcctt
     9961 gtcaatattc gaccgggcat tattcacaac cgcatgaaat attttttcgg cttcgctgaa
    10021 attgaggttg agatattcac attcaatggc ttccttgtat atggcatagg tcagttcgta
    10081 atggcttttc caggcgtatt cgggcaaaag ccccatggcc gttttcaaat aaaatgaagc
    10141 agaactgtac gctgtggatt ttttcgattt ctgcgccgcc tgcagattca acctggccag
    10201 cataatttct tcatctgttc cgctaacgca ccggatgcct ctgttgagct gatcgacaat
    10261 atagaatatt ttttcatgaa gatttttttc atcggttttt ttgagaacat tgccgccgat
    10321 gcgatagtgc atcgcgcttt tttcctcctc ggaaatcatg gaataggcgg cttcctgaat
    10381 ccggtcatgc atgaatttat acatatttcc gtgaagactg atcatatctt cctggattgc
    10441 cgaagtcaga tcaaacagtg tttcgtctat ggattttccg gaaacaaccg acaacaactc
    10501 cagatcaaat cggttgccga tacaggcgca gatttttaat atttcccggg tgttcttcgg
    10561 aaaatgcgat attttttccg ccatgaattc aatcacgttt tcggtaacct gcatctggcg
    10621 tattttgtcg atatcccatt cccatccggt ggaaggattc agttggagca ggcgattatc
    10681 gtaaatattt tttagaaact gaatgacgaa aaagggattg ccggcagttt tcctgttgat
    10741 gagttcggca agcggcattg atttttccgc atcgcacaac agaacattca taatgatcat
    10801 gttgatgccg gtcagattca gaggtcccag gacaatgctg tttattttct tgcctttttt
    10861 gcggatatga tccagcgcga gcgtcagcgg atgcgcatcg ttcgtttcat tatcgcgata
    10921 cgccccgatt agaaacannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat
    11041 tttttcttcg ctttcagaaa tgatttgacg gatgagtccc tggaatgact ggatgatggc
    11101 gctgtacgga acatccttgc gaaactggtc atatttgccg aaaataaaat acccttttct
    11161 ggcaataatc ggtttgtaaa tctcattgac cagcgccgat tttccgatgc ccggctggcc
    11221 ggtcaccagc atgaactcgc ttaatccctt gcaggttctt tcaaagcacc tcatcagttc
    11281 aatccgttca tggtcgcgat ccacaagggt gcgcggaata ttaaaccgga tggaaatatc
    11341 tttggtggca atcggaaaat cgtcaacctg tcctttctgg ttgagttgcc tgaggcattc
    11401 atccagatcc gccatgattc ccagacaatt ctgatatctc tcctcgggat tcttcgacaa
    11461 cattcttaat ataatggaag acagcaccgg cggtatggac gggtttagat ggtccggcgg
    11521 caacggctgt ctcgcgatat gcgaatgaat caattccata ggatctttgg atttaaacgg
    11581 aacctcgccg gtcagcattt cataaaaggt gacacccagc gaatagatgt ccgttcgata
    11641 atccacgccc cggttcatcc tgcccgtctg ctccggcgac atgtaggaca gggttccttc
    11701 gatcacgtcc ggattgtaca gttcgtcatt ggcatgagtc agaacagcgg aaataccgaa
    11761 gtccgcaact ttgacctcat ccctttccgg attaattaaa atattatcgg gcttgatatc
    11821 cagatgaata atattgtttt tatgaatgag ccccagggtc ccggagacct tggaggaaat
    11881 ccgcagtaat gattgcaggt cgagttcttt atcttttaaa gaatccttca gggaaatgcc
    11941 cccgaaatct tcttcaataa tggcatactg gttatcatag tccaccagat caaatatttt
    12001 aacaatgtta tcgacatcaa gagatttgac gagattatat tcctgtttga aacgtgcaat
    12061 ttctgatgac gtcggattga tggtttttag aagctttatg attaagggca aaccttctgt
    12121 ctctttatag cctcgaaaaa tgacagaatt tctggtttcg tgtatctttt cactcaggat
    12181 ataattggca aatttctcca taaagttcag acgaatttaa taaaatggtt tgtattcagg
    12241 aaataattgt ttaatttata acacttttct gtcttttgat caagcattac ctatcattat
    12301 attagaagga tcttcagccc tccacggtaa atgtatagcg cgcagtaatc ctgtaacgac
    12361 cgggactgta cctgtaaaac gcccgcgctt cgtatcgtcc cggtcccgga gaacgaaacg
    12421 tcaagacacc ctgtccacgc cggggaatgt attggtaatc tcccgcatcg gtgtttcttg
    12481 agccggcagg cacgatgcat atccaatccc tggaatgtcc gggcgcgtta taataatgta
    12541 cctgaatttt ctcgccgctt cgatacgtgt ccctgtcagt ctggataatt tgcttagatg
    12601 agtctgcctc tgttttgctt tttccaaaaa tactactgcc agcctttccc acttcgtctg
    12661 aatcatgact ttccagaccg gcttgtgatg ttgtctttga agaatcgccg gataaagtga
    12721 aataagcgat cagtccaata gccatgatca gcaaaatagc aaagattggc cccaggactt
    12781 tgatgaaagg aattttattt atcaaacccg tcgtttctgt ttttgatttg ggcttaaaaa
    12841 tattcaattt aaaattccag ccgtgtttgg cggcaatatc tttcacaaca tccttgagct
    12901 ttccgtattc aaaaccatac ttaccgccga acaataattg tccatgaatt ttctttccga
    12961 catcgattcc cttttgaacg gtcttttctg cgaaaacgcc tgccgtcatt ccggcggaac
    13021 tttctaccat cttttcccag aacgttattt gtttcgttag cgggtcaagt atcgctaaag
    13081 cggtgtattt tatcttcacg tgttgaaccg cagatcgagc tcccgtttcg gaagaaaatt
    13141 caaactgccc cgcagaggat tcccggacag acaaaaattc gttgtttaac gccaaaagct
    13201 gtgttgccac gggtgaggat tccgacgctt gggctttcgg cccttccaga cggcttccac
    13261 agtcgctgca gaaggcgtct tcaggtttat tggatgttcc acaggaaggg cattttttta
    13321 cgttggaatc ttctgcaaga cgttgtccgc aattcgtgca aaaaatggca tttgcttcct
    13381 gaacctctcc gcaggcggaa cattttttca ttttctcaac tcctcatcat cttaataata
    13441 ttgctctcaa tggtattttc ctttatgaaa ctgtttcata atacattttt ttaatactac
    13501 atccggctat atcaactcaa attcaaaagt gctgacgaca cgtatctttt tgacaggcgt
    13561 gcttgtcgga ctgtatggag cggactcatc attgccgtca ctgccgaaaa tctggatatt
    13621 cccctgattg gccgaacgga ttttaccgac acgcgctccc gaatcggagg caaattgctg
    13681 cgctgtcata cgcgcatttt ttgtcgcctc ggcaagaagc ggcggcctca attcattgaa
    13741 tttcgtgatt acgtagcggg gattagccgt tccttcgtcc tgaccatcca gaacaacgcc
    13801 ggatttcagc aattcctcgg tttcacccaa agctttaatg acgagatcga tattcgaggt
    13861 tgttacaacc agagagctgg ttacaacgta acgatatcgt tcggttgcct gactttgatc
    13921 atactcccgc gccaatttat cgatggtgcg ggtcggttga cggctaattt cctcatcctt
    13981 aaatccctgt tttttgagaa aggcaattcc ggcatccctg tcggcgctga ttttggtttg
    14041 cgcatctttg atatcctcgc tcgcccttcg gaaacgcaat acccagatcg actgatcggc
    14101 ctttaccact ttttccacaa gtcctttcac cgtgacggtc cgcgtatccg atttaaaacg
    14161 cgtgccgcct ttgccgatga agtatcctcc gatcg
//
LOCUS       DAOOLB010000051        14178 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4627_length_14178_cov_40.200666, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000051 DAOOLB010000000
VERSION     DAOOLB010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14178
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4627_length_14178_cov_40.200666"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLF77_07330"
     CDS             complement(<1..230)
                     /locus_tag="PLF77_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08702.1"
                     /translation="MKKLMAVLAAMAFVVVMAGGAWADVTGDVDINATVSGKCNAITA
                     NDLTLTIDPATESAVTSTGSATTVQCSKSKAFT"
     gene            complement(312..737)
                     /locus_tag="PLF77_07335"
     CDS             complement(312..737)
                     /locus_tag="PLF77_07335"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPC08703.1"
                     /translation="MNKLQTDILEKGFKVTPQRLAILRYLKENRVHPTAEKIYSEITK
                     EYPAISLTTVYKTLASFVEAGMVKELDIDPHKMRFDICTEDHNHFHCRVCDNVYDVDC
                     DGSTITGDLHSSTKIEGHKVDTVNIHLKGVCRYCEAVSS"
     gene            complement(931..2529)
                     /locus_tag="PLF77_07340"
     CDS             complement(931..2529)
                     /locus_tag="PLF77_07340"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPC08704.1"
                     /translation="MKKVKAESSLKNENKQQFFELDRLILRLSKQFAGSSATEIEKKI
                     GLALQQIVECLDIGRCTFWEVSEDETELRCIRQYVVPKLEKVYGVTPQQSPWISKDQW
                     NGNCSCNVRNEDSVSGAKANKDALLLLGGKSFIISPFHIAGKAVAGMTFSTLEEDREF
                     SPELVSRLNLIGEIITGAFYRKKIEEKYQQSIGDMRQQSGPARAEDFPLREDTKKEFR
                     YQGIIGNSEAMQNVFIKMEQVAPTDSVVLILGETGTGKGVLARTIHELSNRNKKRIVT
                     VNCAALTPNLIESELFGREKGAYTGSTEMQIGRFELADKGTLVLDEFTELPLDLQAKL
                     LRAIQEGEFERLGSPRTIRVDVRILALTSRNLKEEVSKGRFRQDLYYRLDVFPITMPP
                     LRERFGDIPLLVEHFVEQFNRKMQKEIKNIPGGIMAQLMKYPWPGNVRELEHIIERSV
                     IVTHGTSLQLAEKLEDHSLGLMEENAVTNLAEVEKRHIIKILEKTNWRIDGSKGAAVL
                     LGLHPNTLRGRMQKLGIRLQKTPS"
     gene            complement(2619..3740)
                     /locus_tag="PLF77_07345"
     CDS             complement(2619..3740)
                     /locus_tag="PLF77_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08705.1"
                     /translation="MTGKNKNRDQAPDQEQKNPVRYALPVKRPLGTILVDGKFIKPET
                     LDAALDQQKQTRDLLGNILVNMGMLDPADLNAALMVQRDFSTIEDAINAAAGVRMMLG
                     ELLLASSRITRDQIEKALEEQKRTGEKIGEILVRLGLFSSTEMDIALSFQSRQNAGYA
                     SCLSLGELLIAAGYITHDDLEDALERQKGNNRKLGEILVDAGRCKPHHVEHGLRMQKQ
                     LVAAALIAALSLAPVKEAVSSVNPSSTAMTTNIKVTATVLARASMHVLRQPNEIVVTD
                     ADIKRGYLDISAGSLVEIKNNSRAGVDLTFETHGLPFSEVLVSGFGREVALGPNGGII
                     THQLLGANIIAFSYRFVFDRNSRAGTYAWPLVLSVSPVE"
     gene            complement(4015..5496)
                     /locus_tag="PLF77_07350"
     CDS             complement(4015..5496)
                     /locus_tag="PLF77_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08706.1"
                     /translation="MKKKHNTIIRGAGIFLIILFCSWLPSAVLAAEDPFASFLQSNVK
                     QYTATDAEYADRPYLRKLQGAFNADFRILTYGVLQDPAKSTQNPNNDPVGFPNYIGNI
                     DVRPDFRFNTKYLDLVFKPRAMFDYRYWKEGQREGEDDWNDEWYVNEWLVRLKAAERV
                     FLSYGRENLQWGPSFLYSPSNPFFFDNGRSNPQMEVAGMDFARVVFVPHMLWTVSFMV
                     NTDQGRNTLISTDPFEETYAAKIDYTGNQNYASLILSHKDDDTTLGYFGGWTVSDALL
                     LYSEGSMRQGSDALYPNLKRRSLGQSFLEALFAPNSELRPILDRYTSLRSAYMDKTRE
                     DDSKIYPTILVGGAYTFEDIGTLTLEYMYHAQGYDEDEADLYYSLRGRAAYAYLDMGA
                     YPGLLGQFTLYQTGNTGLRFLRKNYTMLQYYKGNIFNRMETTLRWTQNVDDGSAQFLG
                     LLSYSLGNHVELFSSGVLNTGKEDSEFGTFINYQVMFGLKYTL"
     gene            complement(5506..6279)
                     /locus_tag="PLF77_07355"
     CDS             complement(5506..6279)
                     /locus_tag="PLF77_07355"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPC08707.1"
                     /translation="MMSRFLKKNYFTLWSFVVLFFLNVAPLQAMTNQEMLEQADKARG
                     NEEGIQWEIKMHSVEKGRVQDRTLLVIAKGNSASGYNSLIDTLEPGNLKGQKLLMQDR
                     NMWFAKPGLSKPVPISPRQKLMGDASNGDIAATNYAGDYKIIGSEDGQFNNEDCVVLN
                     LQAVDKRATYDRIKYWISKKRVLGVKAEFYTISGKMFKTAIFEYKNRITVEGKSQEFI
                     SQMTITNAIMKDDITVVKYSRPVLKKVSDATFNLNLLTK"
     gene            complement(6301..7539)
                     /locus_tag="PLF77_07360"
     CDS             complement(6301..7539)
                     /locus_tag="PLF77_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPC08708.1"
                     /translation="MMNWMKLAFRNILRNKRRSFVTMVAIGVGFAAIGLYHGYIHNAY
                     AGLRVIAIRGEGLGHLRINKAGWNAKGKLEPEKYMFTREETEKIMKLVGEEKDVVLST
                     PQMQVVGTVTNGATSTVFIAQGVVPKDEVIIKGDWTTFKPRQGHKISDDVVYGVEMAK
                     DLAAYLNMNAGSNGIVMAQTLAGQVNALDMQVSGVYDTGNDFSNDKFMRFNFKFAQSL
                     LDTQSAERVVVLLKEWEDTEMMRDRLLKKLKAAGIDCEIRTWKELSLGYLKMKSYLDM
                     IFIFIASIVLGIVIMTTINTMGMAILERTKEIGTLRALGLKVKGVSLLFTLEGALLGF
                     LGSIVGILLHIVVWVIIRLFPPHYVPPGFSEPVPMFIDMVPWMLLLLVFCFIILSMIA
                     AIIPARGAARKNIVDALGHV"
     gene            complement(7599..8861)
                     /locus_tag="PLF77_07365"
     CDS             complement(7599..8861)
                     /locus_tag="PLF77_07365"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPC08709.1"
                     /translation="MLQWIKLAYRNILRNKRRSVVTMIAIAVGFTSISIYHGYINNAY
                     EGLRWLAICGERLGHLRINKAGWEEQGKLEPHNYMFSKEQTDKIIQLVKEEKDVVLVT
                     PQIQVMGTVTNGLTSIIFIAQGVIPKDEEFITQFWFDVVGYVDEGYDAVIGEKLNESK
                     TYGVLMAKDMASYLKMKPGSDGIVMAQTLSGQMNAMDMQISGVYDTGNDFSNDKFMRF
                     NFNFAQALLDTQSAERVVVMLKHWKDTEQMHDVIAKKLKNAGIDAEIRTWQDLSIAYN
                     KMKSYLDTIFIFLFFIVVTIVLVTIINTMSNTIVERTKEIGTLRALGMKRVGVAKLFA
                     LEGALLGVFGSMAGVVLHTIEWSLIRLFPLHYTPPGLSTPVAMRVNMAPWALMLLIVC
                     FIIISTIAAVIPAKSAAKANIVDSLGHP"
     gene            complement(8871..9545)
                     /locus_tag="PLF77_07370"
     CDS             complement(8871..9545)
                     /locus_tag="PLF77_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC08710.1"
                     /translation="MALIEMKSITKDYHLGETVVHALRGIDLQIDKKEFVAVWGPSGS
                     GKTTLLNLIGAIDDPTSGELAIAGKNVQKLTDDQKSEHRNETIGFVFQSFNLVPVLSA
                     LENVMLPMQIRGASVAEAKKQALMRLEEVGLKDLIHNRPAKMSGGQQQRVSIARALVN
                     NPSLVIADEPTANLDSETAHMVIDIMRELNERDHITFIFSTHDQRLLDKVKRLVRLED
                     GKIIQE"
     gene            complement(9778..10686)
                     /locus_tag="PLF77_07375"
     CDS             complement(9778..10686)
                     /locus_tag="PLF77_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPC08711.1"
                     /translation="MASKFSKNYFVVILCAVFLLFSFNDVFAKSSKKSSHHRKHSTSQ
                     SVQKKKGGLVLRSAAVIVKDQQTGKLLIQKQAKAVMPIASITKLMTAMVVLDAGLDPD
                     ETITIEEEDATAVRGTSFRLAVGTDLTREEALLLALMSSDNRCAHALGRTYPGGYWNC
                     VRAMNAKAAALGLANTRFRDTVGLSSNNVSSAKDLARLVDTAYQYEMIRDFTTRKNAT
                     IFTGWQMLKFRNTNQLVRDGCGWKIGLSKTGFTNEAGRCLVMQAKVAKRPLLIVLLDC
                     RNNKARINDANRIKSWLESKSHSQHT"
     gene            complement(10866..11705)
                     /locus_tag="PLF77_07380"
     CDS             complement(10866..11705)
                     /locus_tag="PLF77_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPC08712.1"
                     /translation="MRVPENWASVIRLASFTIADEARQKSFVAILIICALFILMVRGC
                     YHGDYMMNGQVVDSAKIIIMVSKITFHIIAGGVLFLAALLSMRIFRRDRGDGTQSCIL
                     SKPVSRRQYVSGKILGLWVLLTAVMIVLHGIVFLITSVSANMVMPEFFPASIWCSLNV
                     LFVVMTVLLLSMMMPDIAALLCVIGIGMIGVAADGVFAVRHSPIGQAMMQHDAPSDFS
                     VWQIIYTVWPKLFGVQSFAASRIGAEGFGGPVFAVPILNVGIYCMIIGALLFWRFGKE
                     DID"
     gene            complement(11707..12414)
                     /locus_tag="PLF77_07385"
     CDS             complement(11707..12414)
                     /locus_tag="PLF77_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC08713.1"
                     /translation="MIELDAVTKKYGSLVAVDNVSFSVGAGECFALLGPNGAGKTTIV
                     KLLLDFTRPTAGHLSLNGLSTTNAKSRAFVGYLPENHRIPTHLSGWQYLKRCAALLNL
                     KGTDADKQCQWAVTLTGMQGREHDKSGTYSKGMIQRFGLGAALIGRPKLLILDEPAAG
                     LDPIGIRDIRRLLESLKKDGMTVFLNSHLLSEVEKICDNAAVIRRGRLMVKDKISSLV
                     RDGDTLEDVFIRFVKEG"
     gene            12562..14097
                     /locus_tag="PLF77_07390"
     CDS             12562..14097
                     /locus_tag="PLF77_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HPC08714.1"
                     /translation="MKTFPTLIRILLFFFLTASSCFGAEQVQFRILHLNDFHGFAEPQ
                     RLSGSAQTAGGLSFLAGSINQFRKHQPTLLLAAGDFIQGHPWTNLFEGKSTIEAMNVM
                     GFSAMVLGNHEFDFGQDVLKKRIAEAAFPVLAANVRGFPSVKPYVIKDISGLKVGIIG
                     LITEETSTTTHPKNVEGLIFSPVIDTAQQLVRELRNKTDLIIILSHLGLPADVALAGA
                     VEGIDIIVGGHSHTRVEKPMIVRNTLIVQAWEHGKVLGVLDVTVRNKKISGHEGRLIV
                     IDPVQNKPDPPVAQIVDRYQTQAAGILNHVIGEARVDLPGKESRCRETSLGNLVADIL
                     RQQTGADIALINGGGIRADIWKGPVRMNDILSVLPFSNRAIVLKVSGQEIKDIFEHGI
                     SDPEGKAGRYPQVSGMQLTYNPANPAGQRITGFTINGKPLQTDQRYTLATSDFLAAGG
                     DGYAAFTRILKTMNDGGQNTASENRALFFASGQDIRDMVIDFIRDNKMISAAVENRIR
                     KED"
BASE COUNT         3585 a         3639 c         3227 g         3727 t
ORIGIN      
        1 gtaaaagctt tgctcttcga acattgtacc gtggtagcgc taccggtgga agtgacagcg
       61 ctttctgtcg ccggatcaat tgtaagggtt aaatcgtttg cggtaatcgc attacactta
      121 cctgacacgg tagcgttgat atcaacatca cctgtaacat ctgcccatgc tccgccagcc
      181 attacaacaa caaacgccat tgccgccaaa actgccatta attttttcat aactacctcc
      241 tgatttgtgt taaattgttt ttatgatcct taagtttctc aacttttatt catcttttcg
      301 tcaaagatcc ctcaggaact caccgcctca caatatctgc aaacaccttt caaatggatg
      361 tttaccgtgt ccaccttatg tccttctatt tttgtgctgc tgtgcagatc gcctgttatc
      421 gttgatccgt cgcaatccac atcgtaaaca ttgtcgcaaa cccggcagtg gaaatgattg
      481 tgatcctcgg tgcaaatatc gaatctcatt ttatgggggt cgatatccag ttccttcacc
      541 attcccgcct ccacgaaact tgccagtgtc ttgtagaccg tcgtaagaga gatggccgga
      601 tattccttgg taatctcgct ataaattttt tcagccgtcg gatgcaccct gttctccttt
      661 aagtatctca aaattgccaa acgctgcggc gtcactttaa atcctttttc caaaatatct
      721 gtctgcaact tattcatttc aaatcctatt aagaaacaat cattgttatg ttgtagcata
      781 agcaataggc aggccatatt atatttataa cgtaaaataa ttatatatgc ttgatattac
      841 tgaattataa tcgatgtttc gtttttcaga aaggaggcgg ctgtctttta aataaagtat
      901 ttctacgaaa tacggtatat ttgatgatct ttaggatggg gttttctgca gccggatgcc
      961 cagtttctgc attcgtccgc gcagcgtatt gggatgcaaa ccaagcagaa cggcggcgcc
     1021 tttggaaccg tcgatccgcc agttggtctt ttccaggatt ttaatgatat gccgtttttc
     1081 aacttcggca agattcgtga ctgcattttc ttccatgaga cccagggagt gatcctccag
     1141 cttttccgca agctgaaggc tggtgccgtg cgtaacaata acggaacgct caatgatatg
     1201 ctccagttcc cggacattgc ccggccacgg atacttcatc agttgcgcca tgattcctcc
     1261 gggaatgttt ttaatttctt tctgcatttt ccggttaaac tgttccacaa aatgctccac
     1321 cagcaacgga atatcgccga atctttcccg gagaggcggc atggtgatgg gaaaaacgtc
     1381 caaacgatag taaaggtcct ggcgaaaccg tcctttggaa acttcttcct tcagattcct
     1441 gctggtcaga gcaagaatgc gcacatcaac ccggatggtc cggggagaac caagccgttc
     1501 gaattctcct tcctggatgg cccggagaag tttggcctgc aaatcaagag gaagttccgt
     1561 aaactcatcg agaaccagcg ttcccttgtc ggccagctca aagcggccga tctgcatttc
     1621 ggtggatccg gtataggcgc ccttttcccg gccgaatagt tcgctttcga tcagattggg
     1681 cgtgagagcg gcacaattaa cggttacgat tctttttttg tttctgttgc tgagttcatg
     1741 gatggtacgg gccaaaacgc cttttcccgt tcccgtttcg ccgaggatga gaacaacgga
     1801 atcggtcggc gcaacctgct ccattttgat aaaaacattt tgcattgctt cggaattacc
     1861 gatgatgccc tgatatctga attctttttt cgtgtcttcc cgcagcggga aatcctctgc
     1921 ccgggccgga ccgctctgct gacgcatgtc gccaatcgac tgctgatact tttcctcgat
     1981 ttttttccgg taaaaagcgc cggtgatgat ttccccgatc aaattcagcc gggaaacaag
     2041 ctccggtgaa aattccctgt cctcttccag ggtggaaaac gtcatgccgg caacggcttt
     2101 gccggcgata tgaaaggggc tgataatgaa agacttgccc cccaacagca acaaggcgtc
     2161 cttgttggct ttcgctcccg agactgaatc ttcgttacgg acattgcacg aacaattgcc
     2221 gttccattga tctttggaaa tccatgggga ttgctgaggg gtaacgccgt acaccttctc
     2281 cagttttgga acaacatact ggcggataca gcgcaattcg gtttcatctt ccgatacttc
     2341 ccaaaatgtg cagcggccga tatccagaca ttcgacaatt tgctgcaatg ccagccctat
     2401 ttttttctcg atttcagttg cggaagatcc ggcaaactgc ttggaaagtc tcagaatcag
     2461 tctgtcgagt tcgaagaact gttgcttatt ttcattcttt aaggaagatt ccgctttcac
     2521 ttttttcatt ttatgattgc aaaaaggctg tcatgttaaa atcggctttt tccccgtctg
     2581 aatgatacgt ccaagccgct gcaattcaac cctatctttt attcgacagg actcaccgag
     2641 aggaccagag gccaggcata cgttccggcc cgcgagtttc tgtcgaaaac aaaacgatag
     2701 ctgaacgcaa tgatgttggc gccgaggagc tggtgggtta tgattccccc gttgggtccc
     2761 agtgccactt cccggccaaa gccgctgacc agaacttctg aaaacggcag gccatgagtt
     2821 tcaaaagtca gatcgacgcc cgctcgggaa ttgtttttta tttcgaccag agagccggcg
     2881 ctgatgtcga ggtatccccg tttaatatcg gcatccgtca ccacaatttc gttcggttga
     2941 cgaagaacat gcatgctcgc acgcgccagc acggttgcag tcactttgat attggtggtc
     3001 atggccgtgg aagacggatt taccgaggat acggcttcct tgaccggcgc cagggataaa
     3061 gcggcaatga gcgcagcagc aaccagttgc ttttgcattc gcagaccgtg ctcgacgtga
     3121 tgcggtttac atcggccggc atcaacaaga atttcaccca gtttcctgtt gtttcccttt
     3181 tgacgctcca gagcgtcttc aagatcatcg tgcgtgatgt atcctgcagc gatcaggagt
     3241 tcgcccaggg acaggcagga cgcatacccc gcgttctgac gggattgaaa cgacagcgcg
     3301 atatccatct ccgttgacga gaaaagcccg agccttacaa gaatttctcc tattttttcg
     3361 cctgttcttt tttgctcttc aagcgctttc tcgatctgat cgcgggtaat gcgcgatgag
     3421 gccagaagca gttctcccag catcatccgg acgcctgcgg cagcgtttat cgcatcttca
     3481 atggtcgaaa aatcacgctg aaccatcaga gcggcattca ggtcggcagg atccagcatg
     3541 cccatgttca cgagaatgtt tcccagaagg tcccgggttt gtttctgttg atcaagagcg
     3601 gcatccaggg tttccggctt gatgaatttg ccgtccacca gaatggtccc gagcggcctt
     3661 ttcacgggaa gagcataccg gacaggattt ttttgttcct ggtccggagc ctgatcccgg
     3721 tttttatttt ttccagtcat cttgtatcct ttgggcgggt taaagatcag aatagcttac
     3781 cacggacaat cagtgaaagg ttgaacgcat cgtctctatt tcacactatt agaatactca
     3841 caattgctgc ggttgtcaag aatttggagt ggcggcctga cgttaaattg atttgcatgg
     3901 gaaaggtaat gatgcgcgaa gcggtcgaaa caggcctttt ctgaactata aaacgtaagc
     3961 atcacgaggg ggatgaaacg acggagctat tccatgctcc gtcgtatatt gacgctagag
     4021 cgtatatttc aacccgaaca taacctgata attgatgaag gtgccgaatt ccgaatcctc
     4081 ttttccggta ttcagcacac cggatgaaaa caattcgaca tgattgccga gggaatagga
     4141 taaaagtccc agaaactgag ccgacccgtc atccacattc tgtgtccagc gcagcgtagt
     4201 ttccattcta ttgaaaatat ttcccttgta atactgaagc attgtgtaat ttttacgcaa
     4261 aaatcttaag cccgtattgc cggtctgata cagcgtaaac tggcccagca gtcccggata
     4321 ggctcccatg tcaagatacg cataggcggc ccggcctctg agcgagtaat agagatccgc
     4381 ttcatcttca tcataacctt gcgcatgata catgtattcc agggtgaggg taccgatatc
     4441 ttcaaacgta taggctccgc cgaccagaat tgtcggataa atcttagagt catcctcccg
     4501 tgttttatcc atatacgccg accgaagcga ggtgtatctg tccagaatcg gcctcaattc
     4561 ggaattcggc gcaaacaaag cttccaggaa cgactgaccg agtgatcttc tcttaagatt
     4621 cggatacaaa gcatcgcttc cctgacgcat gctgccttca ctgtaaagca acagggcgtc
     4681 ggaaaccgtc cagccgccga aataccccaa ggtcgtatca tcatccttgt gagataaaat
     4741 caaagaagcg taattctggt ttcccgtgta atctatttta gcggcatatg tttcctcaaa
     4801 aggatcggtg ctgataagag tattacgtcc ctgatccgta ttgaccatga aagagaccgt
     4861 ccagagcatg tggggaacaa acacgacacg ggcaaaatcc attccggcaa cttccatctg
     4921 ggggttgctg cggccgttat caaagaaaaa cggattcgag ggagaataaa ggaaagaagg
     4981 tccccattgc aggttttccc gtccgtagga caagaagact ctctctgcgg ccttcaatct
     5041 gaccagccat tcattgacat accattcatc gttccagtca tcctcacctt cgcgctggcc
     5101 ttctttccag tagcgatagt caaacatcgc ccgcggcttg aaaaccagat caaggtattt
     5161 ggtattgaaa cggaaatccg gtctgacatc aatattcccg atgtaattgg gaaatccaac
     5221 cggatcgttg ttcgggtttt gcgttgattt ggcgggatcc tgaagaaccc cgtacgttaa
     5281 aatccggaaa tccgcattaa aagcgccctg taatttcctc aggtaaggcc ggtccgcata
     5341 ctcggcatcg gtcgccgtgt actgtttgac gtttgactgc agaaaagagg cgaaaggatc
     5401 ttcggcggcc agcaccgctg aaggcaacca tgaacaaaag aggatgatga gaaaaatacc
     5461 cgctcccctg attatggtat tgtgcttttt tttcataaat aacctctatt ttgtcagtaa
     5521 atttaaatta aatgttgcat cggaaacttt tttcagcacc ggccggctgt atttcacgac
     5581 cgtgatatca tccttcatga tcgcattggt gatggtcatc tgggaaatga attcctgaga
     5641 cttaccctcc accgtaattc tgtttttata ctcgaatatc gccgtcttaa acatctttcc
     5701 ggaaatcgtg tagaattcag ctttcacgcc caaaaccctt tttttggata tccagtactt
     5761 gatccggtca tacgtggctc tcttgtccac tgcctgtaaa ttcaaaacga cgcaatcttc
     5821 gttgttaaac tgcccgtctt ccgatccgat aattttataa tcacccgcat agtttgtggc
     5881 cgcaatatcg ccgttggacg catcgcccat cagtttctgc cgcggtgaaa taggaacggg
     5941 tttggacaga cccggttttg caaaccacat attgcgatcc tgcatcagca gcttttgccc
     6001 cttcagattg ccgggttcca gcgtatcaat gagggaatta taaccgctgg cgctgtttcc
     6061 tttggcaata accagcaagg ttctatcctg cacacgtcct ttttccacgg aatgcatttt
     6121 aatttcccac tgaatgcctt cctcgttgcc ccgcgccttg tcggcctgtt ccagcatttc
     6181 ctgattggtc atcgcctgca acggcgcaac attgagaaag aataatacaa caaaactcca
     6241 tagggtaaaa tagtttttct ttaaaaatcg actcatcatt ttctccctga tctataattt
     6301 ttatacatgt cccagcgcgt ccacaatatt cttcctggcc gcacctcttg caggaatgat
     6361 cgctgcaatc atggacaata ttatgaaaca aaataccaac agcagcaaca tccacggcac
     6421 catatcaata aacatgggca ccggctccga aaagccggga ggaacataat gaggcggaaa
     6481 cagccggata atgacccaca caacaatgtg cagcaaaata cccacgatac tgcccagaaa
     6541 tccgagcaac gctccctcca gagtgaaaag caaggaaaca cccttgactt tgagtcccag
     6601 cgcccgcagg gttccgatct ctttggtcct ctccagaatg gccatcccca tggtattgat
     6661 ggttgtcatt atcactatcc ccagcacaat ggaagcaata aaaataaata tcatgtccag
     6721 ataggacttc atttttaaat aacccaaaga gagttctttc caggttctga tttcacaatc
     6781 aatgccggcg gccttcagct tcttgagcag acgatcgcgc atcatttcag tgtcttccca
     6841 ttctttcagc agaaccacca cgcgttccgc tgattgcgta tccagcagag actgcgcaaa
     6901 tttaaaattg aagcgcataa atttgtcatt ggaaaaatca ttgccggtat cgtagacacc
     6961 gctgacctgc atatccaaag cgttcacctg accggccagc gtctgtgcca tgacaatccc
     7021 gttgctgccc gcattcatat tcaaatacgc agccaggtct ttggccattt caactccgta
     7081 gactacgtca tcgcttattt tgtgaccttg acgcggttta aaggtcgtcc aatcgccttt
     7141 gatgatgact tcgtctttgg gcacaacccc ctgagcgatg aaaaccgtag acgttgcccc
     7201 gtttgtcacc gttccgacga cctgcatttg cggtgtggac aagacgacat ctttttcctc
     7261 tccgacaagt ttcatgatct tttcggtttc ttcccgggta aacatgtatt tttcaggttc
     7321 cagcttgccc ttggcgttcc acccggcctt gttaattctc aaatggccaa gtccttcgcc
     7381 gcgaatcgca ataacccgca gtccggcata agcattgtga atgtagccgt ggtaaaggcc
     7441 gatcgcggca aaccctactc cgatggccac catggtcaca aaggatcgcc gcttgtttct
     7501 taaaatattc cggaatgcca gtttcatcca gttcatcata ggtttaatcc agccgccttt
     7561 cagaaatgtt cagtttatct ttttaatcca aaaatatttc agggatgtcc cagtgaatct
     7621 acaatattcg cttttgccgc tgattttgcg ggaatgacgg ctgcaatggt cgatatgatg
     7681 atgaaacaga ctatcaagag catcaacgcc cagggcgcca tattgactct catggccacg
     7741 ggcgttgaca atcccggagg tgtatagtgc agcgggaaga gcctgatcag cgaccattca
     7801 atcgtatgca agacaacgcc tgccatactg ccgaaaacgc ccagcaaagc gccttccaga
     7861 gcaaaaagct tagccacgcc aacgcgtttc atccccaaag cccgcagggt tccgatttct
     7921 ttcgttcttt caacaatggt attgctcatg gtattgataa tcgtcaccag cacgatcgtc
     7981 accacgatga aaaaaagaaa aataaagatc gtatccagat acgatttcat tttattataa
     8041 gcgatggaaa gatcctgcca ggttctgatt tcagcatcaa taccggcgtt tttaagtttt
     8101 tttgcgatga catcatgcat ttgttccgta tctttccagt gtttgagcat gaccacaacc
     8161 ctctccgccg attgcgtatc gagcagggcc tgggcgaaat taaaattaaa ccgcataaat
     8221 ttgtcattgg agaaatcgtt tccggtatca tagacgccgc tgatttgcat atccatggca
     8281 ttcatctgac cgctcaaggt ttgcgccatg acgattccgt cgctgcccgg tttcatcttt
     8341 agataactgg ccatgtcttt ggccatcagc acaccgtaag ttttactttc gttcagtttt
     8401 tcaccgatga ccgcatcata cccctcgtcc acataaccga cgacatcaaa ccagaactgt
     8461 gtaataaatt cctcatcttt cgggataacg ccctgggcaa tgaaaataat ggaggtcaga
     8521 ccgttggtta ccgtgcccat cacctgaatc tgcggtgtaa ccagcacgac atctttttct
     8581 tccttgacca gttgaataat tttatccgtc tgctctttcg aaaacatata gttgtgcggt
     8641 tctagcttgc cctgttcctc ccatcccgct ttattgattc ttaaatggcc caaccgttcg
     8701 ccgcatatcg caagccaccg gagtccctca taagcgttat taatatagcc gtgataaata
     8761 ctgatggagg taaaccccac ggcaatggca atcatcgtca caacagagcg gcgtttgttt
     8821 cttaaaatat tccggtaggc cagcttaatc cactgcagca tcagacgtta tcactcctga
     8881 ataattttgc cgtcttccag acgtaccagc cgtttgactt tatccagaag cctctggtcg
     8941 tgtgtggaga aaatgaacgt gatgtggtct ctttcattca gttcacgcat aatatcgatt
     9001 accatatggg ccgtttccga atcgagattg gccgtcggtt catcggcaat aaccagtgag
     9061 ggattattga ccagcgcccg ggcaattgaa actctctgct gctgaccgcc cgacattttc
     9121 gccggacgat tatgaatgag atccttcagg ccgacctctt ccagacgcat cagcgcctgt
     9181 tttttggctt ccgccaccga cgcgcctctg atctgcatgg gcagcatgac attttccagt
     9241 gcggagagaa caggcaccag attaaagctc tgaaaaacaa aaccgatggt ctcattccga
     9301 tgctcgcttt tctgatcgtc cgtcaacttc tggacatttt tccctgcaat ggccaattct
     9361 ccggatgtgg gatcatcaat ggcaccgatg agattcagga gggttgtttt gcccgatccg
     9421 gacggtcccc aaacggccac aaattctttc ttgtcgatct ggagatcaat gcctctcagc
     9481 gcatggacaa ccgtctcgcc aagatgatag tccttggtga tactcttcat ttcaattaaa
     9541 gccattttat accccctgga aaaactcaac ggcaaaccgt catggattgc ggccgacact
     9601 ttgcaaagca atcatttttt tggcttgcgg agcatataaa gaaatgtcct atatgtcaat
     9661 taaatatcaa aaaaaaatag ataaaaaaat aaaggcggtg gttaggatgt tattaatttt
     9721 caattgaaaa ccgcttcaca ccggctctca atttcaaccg atacgatata cccattatta
     9781 tgtatgttga gaatgtgatt ttgattccaa ccaggatttt atccggttgg catcgttaat
     9841 gcgtgcctta ttgtttcggc aatcaagcag aacgatcagc agcggacgtt tcgccacttt
     9901 ggcctgcatg accagacacc ttccggcttc attggtaaat cccgtttttg acagaccgat
     9961 tttccagccg caaccgtccc ggacaagctg gttggtgttt cggaatttca gcatttgcca
    10021 gccggtaaag atcgtggcat ttttacgggt cgtaaaatca cggatcatct cgtactgata
    10081 ggcggtatca accagtcgcg ccaggtcttt tgcggaagaa acgttattgc tcgacaggcc
    10141 aaccgtatcc ctgaaacgcg tattggctaa tcccaacgca gcggcttttg cgttcatcgc
    10201 ccggacacag ttccagtaac cgcccggata cgttctgccc agtgcatgag cgcaccggtt
    10261 atccgaagac atcagcgcca gcaataacgc ttcctcgcgg gtgaggtcgg tgcccacggc
    10321 aagccgaaag cttgtcccgc gaaccgctgt cgcgtcttcc tcctcaatgg tgatcgtttc
    10381 atcaggatcc agtccggcgt caagaaccac catggcggtc atcagtttgg tgatagaggc
    10441 aatgggcatg acggctttgg cctgtttctg aatcaacaac ttaccggttt gctgatcctt
    10501 cacaatcact gccgccgaac gcaaaaccag cccgcccttt ttcttctgga cggattgtga
    10561 tgtggaatgt tttcgatgat gggacgattt tttggaggat tttgcaaata cgtcgttaaa
    10621 ggaaaataat aaaaaaactg cacataaaat tactacaaaa taatttttac tgaatttact
    10681 tgccatgcat tctcccgatc ttcaagatac gtctttagtt ttgcaacaaa gacctgagtg
    10741 tcaatttaat gccggcattt gctctgccaa cttgtgtttc aaagttaatt ttttggaatc
    10801 atacagaatt ttttcgaatt agtcaatttt gaatttgatt tttttgcaaa aaaatcccag
    10861 ggatgtcaat caatgtcttc ttttccgaaa cgccagaaca gcagtgcgcc gataatcatg
    10921 cagtagatgc cgacattcag gatcgggacg gcaaaaaccg gtccgccgaa tccttcggcg
    10981 ccgatccggg aagcggcaaa ggactgcacg ccgaaaagtt tcggccacac cgtataaatt
    11041 atttgccaaa cggaaaagtc agatggcgca tcgtgctgca tcatcgcctg tccgatcgga
    11101 ctatggcgaa ccgcaaagac gccgtcggct gcaacaccga tcatcccgat gccgataacg
    11161 caaagcaacg cggcaatatc aggcatcatc atcgacaaaa gaagcacggt catcaccacg
    11221 aaaagcacat tcagcgagca ccaaatcgac gccggaaaaa attccggcat gaccatgttg
    11281 gcgctgacgg atgtgatcaa aaaaacgatg ccatgcagaa caatcatgac ggccgtcagc
    11341 aaaacccaca ggcctagaat ttttccggag acatactgcc ggcggctgac gggttttgaa
    11401 aggatgcagg attgcgtgcc gtcaccgcgg tcgcggcgga aaatgcgcat ggacagcaga
    11461 gccgccagaa acaaaacgcc tccggcaatg atatggaatg tgattttcga aaccatgatg
    11521 atgatttttg ctgaatcgac gacctgtccg ttcatcatgt aatcgccgtg ataacagccg
    11581 cgcaccatca aaataaacaa agcgcagatg atcagtatcg cgacaaaact tttttgacgg
    11641 gcttcatccg caatggtgaa tgaggccagg cggatgacgg aagcccagtt ttccggaact
    11701 ctcatgtcaa ccttccttca caaaacggat aaaaacatct tccagggtat cgccgtcccg
    11761 gacgagggaa gatattttat ccttgaccat cagccttccg cgacggatca ctgcggcgtt
    11821 atcgcatatt ttttccactt ccgacaaaag atgggaattc agaaaaaccg tcataccgtc
    11881 ctttttcagc gattccagta accggcgtat gtcccggata ccgatgggat caagtcctgc
    11941 ggcaggttca tccaggatca gcagtttcgg ccggccgatc agagcggcgc ccagaccaaa
    12001 tcgctgaatc atacccttgg aataggttcc ggatttgtca tgttcccttc cctgcatgcc
    12061 ggtcagtgtg accgcccact ggcactgctt atccgcatcg gtacctttga gattgagcag
    12121 tgccgcgcag cgcttcagat actgccaccc ggaaagatgc gtcgggatac ggtgattttc
    12181 cggcagatag ccgacaaaag cgcggctctt cgcgtttgtt gtggaaagac cgttcaacga
    12241 caaatgtcct gcagtcgggc gggtaaaatc aagcagcagt ttaacgattg tcgttttccc
    12301 cgctccgttc ggtccgagaa gagcaaaaca ctcgcctgcg ccgacggaaa atgaaacatt
    12361 atccacggca accagggagc catacttttt tgtcacggcg tcgagttcga tcatcacgaa
    12421 tgaacatatg gtccgaagga cattctgtca acaaaattat cataaaaacg cctgccgctc
    12481 tttccgtccc gtaaacagct ctggaattct tccggccatt gggctatatt aaaaaaagtt
    12541 tggaaaagaa ggaatttttc catgaagaca tttccgacat tgatccgcat tcttttgttt
    12601 ttctttctga ccgcttcatc ctgtttcggt gcggaacagg ttcagtttcg gattctgcat
    12661 ctgaatgact ttcatggttt tgccgagccg caacgcctgt ccggatcggc gcagaccgcc
    12721 ggcggattat cttttcttgc cggatcaatc aatcagttca gaaaacatca gccgacactg
    12781 cttctggctg ccggagactt tattcagggg catccgtgga caaacctttt tgaaggcaaa
    12841 tccaccatcg aggcgatgaa tgtcatgggg ttttcggcaa tggtcctcgg aaatcacgaa
    12901 ttcgatttcg gacaggatgt tctcaaaaaa aggattgccg aagcagcttt tccggttctg
    12961 gccgccaatg tccgcggttt cccgtccgtc aaaccgtatg tgatcaaaga catctccggc
    13021 ctgaaagtgg ggatcatcgg attgatcacg gaagaaacat ccaccacaac gcatccgaaa
    13081 aatgttgaag gcctgatttt ttctccggtc attgataccg cgcaacaact tgtccgcgag
    13141 ttgagaaata agaccgattt gatcatcatt ctttcccatc ttggcctgcc ggccgatgtg
    13201 gccctggccg gggccgttga aggaattgac atcattgtcg gcggacattc gcatacccgc
    13261 gttgaaaaac cgatgatcgt tcgtaacaca ctgattgttc aggcgtggga acacggcaaa
    13321 gtgctggggg ttctggatgt aacggtccgc aataaaaaaa tcagcggaca tgaaggacgg
    13381 ctgatcgtca tcgatccggt ccaaaacaag ccggatccgc cggtggcaca gattgttgat
    13441 cgctatcaga cacaggcggc cggcattctc aaccatgtga tcggcgaagc ccgggtcgat
    13501 ctgccgggga aggagtcccg ctgccgggaa acttctctgg gcaatctggt cgccgatatt
    13561 ttaagacagc aaaccggcgc cgatatcgct ttgatcaacg gcggcggcat ccgggcggat
    13621 atctggaagg gtccggttcg tatgaacgat attctttccg tcctgccttt ctccaatcgc
    13681 gctattgtcc tgaaggtcag cggacaagaa atcaaagata tcttcgaaca cggcatttcc
    13741 gatccggaag ggaaggcggg acgttatcct caggtttccg gaatgcaact gacctacaat
    13801 cccgccaacc ccgccggtca gagaattacc ggctttacga tcaacgggaa accgttgcag
    13861 accgaccaac ggtacacact ggcgaccagt gattttctgg ccgccggagg cgacggctat
    13921 gccgcgttca cccgaattct taagaccatg aatgacgggg gacaaaacac cgcatcggaa
    13981 aatcgcgcgc tgttttttgc atccggacag gacattcgcg acatggtcat tgattttatc
    14041 agagacaata aaatgatttc cgccgccgtg gaaaacagga tcagaaagga ggattgattg
    14101 atgcgtgtct ggttaaataa tgaaccggtc gatgtggttc ccggaatgaa agttcgcgaa
    14161 gccctgattc aagcaggc
//
LOCUS       DAOOLB010000052        14039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4699_length_14039_cov_38.903390, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000052 DAOOLB010000000
VERSION     DAOOLB010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 14039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14039
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4699_length_14039_cov_38.903390"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..644)
                     /locus_tag="PLF77_07395"
     CDS             complement(126..644)
                     /locus_tag="PLF77_07395"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC08715.1"
                     /translation="MNTNEDIERFRKLAETGDADAQYHLGLIYQNEKGVSLDYAQAVK
                     WLVMAAEQGHADAQYNLACIYNNGKGVSLDYPKAIKWFTRAAEQGHAHAQYMLGLMYS
                     SGLQDYVEAARWFLKAAEQGIADAQFKIGVILEEGRGVRKDPASAEVWYGEAAEQGHA
                     DAKKRLDKMQNK"
     gene            885..1310
                     /locus_tag="PLF77_07400"
     CDS             885..1310
                     /locus_tag="PLF77_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPC08716.1"
                     /translation="MNTKIGLSQILLYFLMLMFMMVIGCGDGADDFKLPKYHDADADK
                     CTISGRVTYKGVGRAGITVLLSGYSNATVATDENGEYRFDDVDGDQAYVVSPSSGTLI
                     FTPESIGVEPDGSNVDGVDFDAMDCCGDDTSEEEETEEI"
     gene            complement(1456..>1958)
                     /locus_tag="PLF77_07405"
     CDS             complement(1456..>1958)
                     /locus_tag="PLF77_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08717.1"
                     /translation="IMPGTDQIRLRIPINLRYIHPDVDPLYIGNAVFSTVIGFSRAEI
                     NRMSVYRIACRLKEAIAKMRNEHYVRSIACLSKFGIRYRGDIFKTLPPYDPDTDILAA
                     NLTHVNDLESLGLGDDVGRILYMGSTMQTSFTMLKEKHNRLFAEIRSRYPLESPKTIH
                     GTAEVP"
     assembly_gap    1959..2058
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2059..2590)
                     /locus_tag="PLF77_07410"
     CDS             complement(<2059..2590)
                     /locus_tag="PLF77_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPC08718.1"
                     /translation="MIRYISSTTFSPKDHYGIDILPLSFFENADYPGSPPIECLVLYK
                     KKTSLPAARESFLETIRHYNLFSSRLIMIDENRFALLYCTDGFQYMVLPRLDADAAHI
                     SIDDVRHMMRHVKTLPGEPLFAVTIIPVRDGLLGGISCSHAIGDAVSLMLFLYAWGCI
                     HYGNSFSKPSTQRLFSG"
     gene            complement(2638..4890)
                     /locus_tag="PLF77_07415"
     CDS             complement(2638..4890)
                     /locus_tag="PLF77_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC08719.1"
                     /translation="MLKLFPPDDPLQSVRIKRFLMAFASYSVWLTIALITYLLGIAPV
                     SFHVLFICFLGILLCNILIYAAIRSGFNKRFDDPSLTLFQMIIATFWAMVILYYTDEA
                     RGAVLIVYLVVFVFGLFKLNLRQFFYLSVFAVLNYALVLFLLYKNHPESLNIENEILG
                     LIVLALVLPWFSFMGGYITRLKKKVTHSLAQTKEMELKFRTIFDSASDGITMLDTRQG
                     TFVAANATLCQMLGYTQEEFLKLKISDLHPKESQKDILNIYKKINHHELSIARNIPLL
                     KKNQTILYADISGSLISLEGREYSVGVIRDITERKFAEELLIASEQRYRLLADNVTDI
                     IFTMDMNLRYTYLSPSGYRLSGYNEDEIARMNISNMVEPETYFRFAEVLKEELVLDRQ
                     SGRDLKRSRTLEYQHIFKDRSKKWMEATLTFLRDKNGEAVGIIGIARDISERKAMEEK
                     LQFEERRFRNFVEHSADIIALVNLEGLITYVNPAIERVLGYKTEERIGRRGFELVHPD
                     DLEFLIQSFTTLATQPDSPPIHGEMHLRHKNGTYRTLEAVGSNLVKDHVVEAIIINYR
                     DITERKLAEEALQRSEQRYLELSILDDLTQVFNSRHFHAELGKEMERSTRYGHPLSLL
                     LLDLDHFKTFNDTYGHVEGDNVLSRLGQVVKKCLRDSDSAYRYGGEEFTIILPMTTKE
                     DAVSTAQRIQNEFRKEVFTPVPGKEIFVSMSIGASQYIPKEEMKSFVHRVDQFMYQAK
                     QNGRDRICSD"
     gene            5100..5486
                     /locus_tag="PLF77_07420"
     CDS             5100..5486
                     /locus_tag="PLF77_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08720.1"
                     /translation="MSHKLKKESDDAGIVITFSGMVEADEVDNIQEQINSGADFPQLR
                     YQIWDFSKAEEINISIDQIQDIAMHTALASSKNPKLRIAIIPRKTSHNVLGKTFHTFE
                     KVWGSYESKSFRDIHAAREWGQSGKT"
     gene            5726..6025
                     /locus_tag="PLF77_07425"
     CDS             5726..6025
                     /locus_tag="PLF77_07425"
                     /inference="COORDINATES: protein motif:HMM:NF015893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HPC08721.1"
                     /translation="MQKKVGINERDYFKEPFTRDEIKALLQGKPASDMFSFKSPSFKA
                     LGLNPAGLHDKDLMNLMLREPRLIRRPVVKIGRHVYFGADIKVLNEIIRQSIENG"
     gene            6045..6518
                     /locus_tag="PLF77_07430"
     CDS             6045..6518
                     /locus_tag="PLF77_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPC08722.1"
                     /translation="MPKKTSAGIVLYRFVEEDMEVFLVHPGGPFWAKKDKGAWSIPKG
                     LFEPGEDPLEAARREFYEETGHAVSGEFRALMPLKQPSGKVVHAWVVEGHIDEKSIKS
                     NMFSMEWPPRSGKEQSFPEVDRGGWFTLRIACEKILPGQRGFLDELQTKIKNRPA"
     gene            6654..7115
                     /locus_tag="PLF77_07435"
     CDS             6654..7115
                     /locus_tag="PLF77_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HPC08723.1"
                     /translation="MDQKIICLSAGLSQDEIEKGKALYFAASEGQSTLEVTAMSRSML
                     NMKIIEILACLMEELEKDPAEKQNDSGDVIPADHCKTGVLVMLAQDRELVLQVMRIFK
                     AVLPDPRNVIFAIITETALKWTLAEYLEHLSREHEYMQTRRPENNPDMKKL"
     gene            7140..8447
                     /locus_tag="PLF77_07440"
     CDS             7140..8447
                     /locus_tag="PLF77_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPC08724.1"
                     /translation="MIYNEEYETLPREALEALQLKRLQQVVNRVYHHVGFYKKSFDKA
                     GVHPNDIRTLDDLKRFPLTTKQDLRDNYPFGMFATPMSNIVRLHASSGTTGSSTVVAY
                     TKRDIESWAELVARCFAAADLTRGDIIHNAYGYGLFTGGLGVHYGAEKLGASVIPMSG
                     GNTKWQLMILQDFGPTAICCTPSYALNLAEQGRTMGIDMPSLKLRVGIFGAEPWSEQM
                     RAQIEEAFAIKAMNIYGLSEIMGPGVSMECSEAQAGMHVFEDYFLVETIKPDTGEVLP
                     PGEQGELVFTTLTKEAFPLIRYRTRDISRLITTPCRCGRTFHRMERVMGRSDDMLIIR
                     GVNVYPSQIESVLINIEGLEPHYQLIIERSGTLDTLEVQVEVTEGVFAEAGEVKGLQQ
                     LEKRLIKDMKDYLGITAKVKLVEPKSIMRSEGKASRVIDKRKI"
     gene            8505..9266
                     /gene="hisF"
                     /locus_tag="PLF77_07445"
     CDS             8505..9266
                     /gene="hisF"
                     /locus_tag="PLF77_07445"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HPC08725.1"
                     /translation="MEPIKIMPCLDMKNGRVVKGVHFIDIREAGDPVENAEFYQAEGA
                     DELAMLDIAATLENRKTRLEWVKNVSKVIRIPLTVGGGIASLEDIDMVLGAGASKVSM
                     NSAAVKNPELIRQAAEKFGKEKITVAIDAKRNAQMPSGFELVVSGGTMPIARDAVAWA
                     KICQEKGAGVILPTSMDGDGTKAGYDIPFTRAIADAVTLPVVASGGAGKLEDFYEAVV
                     QGGATVLLAASVFHFRLLKIRDVKTYLREKGLAIA"
     gene            9278..9889
                     /locus_tag="PLF77_07450"
     CDS             9278..9889
                     /locus_tag="PLF77_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08726.1"
                     /translation="MSKKNNAGFMYLALLGAAITTLCDGVHVYTQTLSYTTPFLFGQA
                     WWVFPGFVLAFLFMGGVYLGVVNRLPLFFAVKKSISGGSLHDVVETATAFALIYVMSG
                     LGSSEPLLLSAVFYGTFVLRLAFSYDRLFLLLLAVLMALGGMFVESLYAAAGLMAYRY
                     PDVFYVPFWLGGLYVHGAFALREGMRFFVYGRKPGTLISVSPL"
     gene            10036..11235
                     /locus_tag="PLF77_07455"
     CDS             10036..11235
                     /locus_tag="PLF77_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPC08727.1"
                     /translation="MMNEDKKTSWRNKKSDLRRLVLLMNRQNNRFWPPLDPILRTVDL
                     VIEPEELNLLLKMGTDLYTYDQVALLSGMNSEKFDAVFDSLKKKAFIGIKNPETSGER
                     YTLHPFVVGWFEGQVSYLIGKPEEKEFAKRYMGFLDSLRKYNFFPLRNIMNVIGKHAT
                     VSNQSVGTVRESRNGQGKSKIVVDENIVVPDSGIYPTSSVNDLIMEFGTRSIIGQFKV
                     CMCRQMTANVDQPCRLKMPDDVACMGFGEGIKTYMKYAYMRQISKEQAFDVIQKARDH
                     GAVHTVFHEMDDTKLPQIGLCNCCWDCCGIFRTYNMGVVPLRYRCYYIARIANSDRCT
                     GCGRCEKFCPTSAINVVAKSVVLDTKKCIGCGQCVHQCVHAVVELVEDRRTAFLPMLK
                     TSEARLL"
     gene            11280..11897
                     /locus_tag="PLF77_07460"
     CDS             11280..11897
                     /locus_tag="PLF77_07460"
                     /inference="COORDINATES: protein motif:HMM:NF014693.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HPC08728.1"
                     /translation="MPDLKKKQHLPDDLKKCVEFHGHICPGLIYGYRVAQEAIRLMHI
                     GRSVDEEVVAVCENDSCAVDALQVMLGTVAGKGNLLIKNYGKNAFTVMSRADRKAYRF
                     SRKKQYAYGGSSGRRFEKLERAVASGDASEEEKQELKRLKIDDLLARPFSEIFTTEEV
                     VFDGPGYAPLAPSEPCAVCGEMTMATKMIPLRDGRQVCIPCSEKS"
     gene            11967..12266
                     /locus_tag="PLF77_07465"
     CDS             11967..12266
                     /locus_tag="PLF77_07465"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPC08729.1"
                     /translation="MKKIGITILVVLGVLITSIACAANLYGIVSDKNGKPVEVKVTLK
                     DDKGAAVGQPVTTDKKGAYAFKDIKPGSYVVVVGAKDEWKIFVGPGETRRDFSLK"
     gene            12291..13319
                     /locus_tag="PLF77_07470"
     CDS             12291..13319
                     /locus_tag="PLF77_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08730.1"
                     /translation="MAERDIQKQEEPTEAPRKKDLWDILQALGPALIAAAVAVIGSVY
                     SYQQAQMTKASEMREVYTKIMTERENSDNNIRASMFQMLFNAMFDKDINSADTPDNVN
                     QIKKRVMFLDVLSRNFDTVDIKPLFEDLDGELTRKMYNDRSYSDGAQEDYFALRSELR
                     RIGRNLAVKQLNALASLPGTMVKTIVLIEDKDKNIMVMQDETTANGNGDTLPVEIKAQ
                     NLDDGKVEIAIDYQKAKASDKGFKAPSFIITFYDMPYIDNSVLDKDMRVGVVLTKYAT
                     TREIDLFRDRIDRRLLKDYEDLKAGGIAKYAELRLIKFPAKYTGHRDRPYLQEILQNL
                     VENEKSVR"
     gene            13416..>14039
                     /locus_tag="PLF77_07475"
     CDS             13416..>14039
                     /locus_tag="PLF77_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HPC08731.1"
                     /translation="MKKYVPVLLLLIFVFACWACVPVPVPVGGTTTVYDESYGYEEPY
                     GYDGYVDVPVVYGEPMYYAPPVSVTFAFDYFTYEAIGGFVDVVFWRGGNRYHREPWYE
                     QGRRITDRDIRSGKRYHKVRGKEFYNHREKLRSKHNIVHPDSYYKLQRSKQKEQQRRL
                     EEQKKRELEMRKQREKQRLEEDRNRYNREKRELQEKQRRLEEQKKREL"
BASE COUNT         3674 a         3114 c         3695 g         3456 t
ORIGIN      
        1 gacgcctgtc tgaaactctt tagagagaag ggctacaaac ttatataaaa ggaaaaaact
       61 gaaatttcgg gtgtgatcct tctcgaggtt tcatccggct tttttatgga tgaacatcgc
      121 cgccgctatt tgttttgcat tttgtccagt cgttttttgg catccgcgtg gccctgctct
      181 gcggcttcgc cgtaccacac ttcagccgaa gcagggtctt ttctgacgcc tctgccttct
      241 tccagaatca cgccgatttt gaattgcgcg tcggcaatgc cctgttccgc tgcctttagg
      301 aaccatcgcg cggcttcaac ataatcctgt agcccgctgc tgtacatcaa tcccagcata
      361 tactgggcat gggcatgacc ctgctcagca gcccgggtaa accatttgat cgccttcggg
      421 taatccaacg agacgccttt gccgttgttg taaatacagg ccagattata ctgggcatcg
      481 gcatgtccct gctcggcggc catcacaagc catttaacag cctgagcata atccagagat
      541 accccttttt cattctgata tatcaagccc agatgatact gggcatcagc atcaccggtc
      601 tcagctaatt ttctgaaacg ctcaatatct tcattcgtat tcattttgaa ataccacacc
      661 ttttcatttg agagaaacat tatcataatt gatagcggaa atctatacgg atctgcttga
      721 tgatgcatgt gaaaatcccc ggggcttcca tgatggatga aaacggtctc tgtcgaaatt
      781 atacgcataa ccattccttg acattcaaac ggtaattcca tataaataaa tatatcaaca
      841 aaagtttcag caaattcgat tagcgtcatc tttgggggat ctttatgaat acaaaaattg
      901 gtctatctca gattttattg tattttctta tgctgatgtt catgatggtc ataggttgcg
      961 gcgacggggc ggatgatttt aaattaccga aatatcatga tgccgatgcg gacaaatgca
     1021 ccatttcagg cagagttacc tataaaggtg tcgggcgtgc gggtattacc gttctcctgt
     1081 cggggtacag taacgctact gtagctacag atgaaaatgg agaataccga tttgacgacg
     1141 tcgatggcga ccaggcctat gttgtcagcc cgagcagcgg aacgctgatt tttacacccg
     1201 aaagcatagg cgtggaaccg gacggatcaa atgttgacgg tgtggatttc gatgcgatgg
     1261 attgctgcgg tgacgataca tccgaggaag aggaaacgga agaaatctga tcaaccacgg
     1321 caattggtgg atcatgccct cgcttagtat cggcggtttg caggacatcg gaatgtcttt
     1381 acttaaaatc ataaatatca cggtataagt ctcgatgcgc tgccgtaaat gaccgatgtt
     1441 gtcgcaggcg tgctgttatg gaacttcagc agttccgtga atcgtttttg gactttccag
     1501 cgggtatctg cttcttattt ctgcaaataa tctgttgtgt ttttccttga gcatggtaaa
     1561 acttgtctgc atcgttgaac ccatgtacag aattcttccg acatcgtctc ccagtcccag
     1621 cgattccaga tcgttcacat gcgtcagatt ggccgcaaga atatccgtgt cgggatcata
     1681 gggcggcagc gttttaaaaa tgtcgccccg ataccttatc ccgaatttcg acagacaggc
     1741 gatgctcctg acataatgtt cattcctcat ttttgctatg gcctccttaa gacggcaggc
     1801 aatccgatag acggacatcc ggttgatctc cgctcgggaa aagccgataa cggtggagaa
     1861 aaccgcgttg ccgatgtaca gcggatcaac atcgggatga atatatcgca gattgatggg
     1921 gattctcaat ctgatctgat ccgtgccggg catgatgcnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnntc cccgaaaata atcgttgcgt cgacggtttg gaaaaactgt
     2101 tgccgtagtg aatgcacccc caggcataaa gaaaaagcat cagtgaaaca gcatcgccaa
     2161 tggcgtgaga acaactgatc ccgcccagca atccgtctct taccgggata atggtgacgg
     2221 caaacaaagg ttctcccggt aatgttttga catgtcgcat catgtgtcgg acatcatcga
     2281 tcgaaatgtg agccgcatcc gcatccagcc gcggtaaaac catatattga aaaccgtccg
     2341 tacagtacag aagcgcaaac ctgttctcat caatcatgat caggcggctg gaaaacagat
     2401 tgtaatgtct gatggtttca agaaaacttt ctcttgccgc cggaagagat gtttttttct
     2461 tgtaaagaac cagacattcg attgggggac tgcccggata atctgcattt tcgaaaaaag
     2521 acaagggcag tatatcgata ccgtaatggt ctttcgggga aaacgttgtg gatgaaatat
     2581 atcttatcat gacttggatg ccggtgaaaa gccttggatg acgggtaacg aatgctgtca
     2641 atcgctgcag attcggtccc tgccgttttg tttggcctga tacatgaact ggtcgacacg
     2701 atgaacgaaa cttttcatct cttctttcgg tatatattgc gaggcaccga tactcatcga
     2761 cacgaaaatt tcttttcccg gcacgggggt aaagacctct ttcctgaatt cattttggat
     2821 cctctgggcc gtactcacgg catcctcttt tgttgtcatg ggcagaataa ttgtaaattc
     2881 ctcgccgccg taacggtacg ccgaatccga atcgcgcaag cattttttga cgacctgacc
     2941 gagtcgggat agaacattat caccctccac atgaccgtag gtgtcgttaa atgtcttgaa
     3001 gtgatcaaga tccagaagca ggagcgacaa tggatgaccg tagcgggttg accgttccat
     3061 ttcctttccc agttcggcgt gaaaatgtct ggaattaaaa acctgtgtca ggtcgtcaag
     3121 gatgctcaac tccagatatc gctgttcgct tctctgcaga gcttcctctg ccagtttgcg
     3181 ttcggtgata tcccggtaat tgataataat cgcttccacg acatgatctt tgacgagatt
     3241 gcttcccacg gcttccaatg tgcgatacgt gccgtttttg tgacgcagat gcatttcgcc
     3301 gtgaatcggc ggggaatcgg gttgggtggc caaggtcgta aacgattgga ttaaaaattc
     3361 caggtcatcc ggatgaacaa gttcgaatcc tctgcgtccg attctttctt ctgtcttata
     3421 tcccagtacc cgttcaatgg ccgggttgac atacgtgatc agtccttcca gattgaccag
     3481 cgcaatgatg tcggcagagt gctccacaaa attccggaat cgccgttctt caaactgtaa
     3541 tttttcctcc attgccttgc gttcggaaat atcccgggcg atgccgatga ttccaacggc
     3601 ctcgccgttt ttatccctta gaaaggtcag ggtggcttcc atccatttct ttgatcgatc
     3661 cttgaagata tgctgatatt ccagcgttcg cgaccgtttt aaatcccgtc ccgactgcct
     3721 gtccagcacc agctcttcct tcagaacttc ggcaaaacgg aaataagttt ccggttcgac
     3781 catgttggaa atgttcatcc gggcgatttc gtcctcgtta taccctgaaa gacggtagcc
     3841 ggaaggactg aggtaggtgt atctcaggtt catatccatg gtaaaaatga tgtcggttac
     3901 gttgtcggca agcaggcgat aacgttgctc gcttgcgatc agcaattcct ccgcaaactt
     3961 gcgctctgta atatctctga tgacacccac ggagtattct ctgccttcga gggagataag
     4021 cgatccgctg atatcggcat ataagatggt ttgatttttt ttcaggagag ggatgtttct
     4081 ggcaatcgat aattcatgat gattgatctt tttatagatg tttaaaatat ctttttgcga
     4141 ttctttcgga tgcagatcgg atattttcag cttaagaaat tcttcctgag tatagcccag
     4201 catctggcaa agtgttgcat tggcggcaac gaaagtcccc tgtcgggtat ccaacatggt
     4261 aatcccgtca gaagcagaat caaagatggt tctgaatttc agttccattt ctttcgtctg
     4321 ggcaagggaa tgcgttacct tctttttcag tcgggtgatg tatccgccca taaaggaaaa
     4381 ccagggaaga accagtgcca gaacgatcag ccctaaaatt tcgttttcga tgttcagaga
     4441 ttcggggtgg tttttgtaga gaagaaacag aaccaaagcg taattcaaca cggcaaagac
     4501 ggataaataa aaaaactggc gcaaattcaa cttgaaaagt ccgaaaacaa aaacgactag
     4561 atagacaata agaacagcgc cccgggcttc atcggtataa tacaaaataa ccattgccca
     4621 gaacgtggcg atgatcatct gaaacagtgt caggctcggg tcgtcaaatc gtttattgaa
     4681 gccggatcgg attgcggcat aaatcagaat attgcataaa agaataccca ggaaacaaat
     4741 aaaaagaaca tgaaacgata ccggtgcaat gcccagcaga taggtgatca gcgcaatcgt
     4801 caaccacacg gaataagaag caaaggccat aagaaatcgt ttgattctta cagactggag
     4861 aggatcgtcg ggcggaaaca gtttcaacat gaccaatcgc ctcaaagtgt cgaattttgt
     4921 aattgatttt ttttaccaca aaataaactc aagttcaata tatcgctcga tgtatttaga
     4981 agatcgatgg aggcgcctga cgccttcagg taaaatatac ggtaaaattt tcgtatctgt
     5041 gctattaaaa aagggtaaca gttcgtacaa cacttgacat caactcgggg gtcgtcatca
     5101 tgtctcataa attgaaaaaa gaaagtgatg atgccggtat cgtcattact ttttccggca
     5161 tggtcgaggc ggacgaagtc gacaacattc aggagcagat caattccggt gcagattttc
     5221 cacagttgcg ttatcagata tgggattttt ccaaggccga agagatcaat atttccatcg
     5281 atcagattca ggatatcgcc atgcacaccg cgctggcatc cagcaaaaat ccgaaactga
     5341 gaatcgccat cattccccgc aagacatcgc ataacgttct gggaaaaacc tttcacacgt
     5401 ttgaaaaggt gtggggatcc tatgaatcga aaagtttccg ggatattcac gctgctagag
     5461 aatggggcca aagcggcaag acgtaaccgc ttgtccggtc cggtggtcct ctgcctgtgt
     5521 ccggaaatag atttgcagat ggtcgatatc gcgggcatcg ggggatagac acggatatga
     5581 gccttcccgg gatattattt cgacaggaac ggatcgttca caggtcatat gtcccgtggg
     5641 gaatggataa ggagagctta aaatgaaggt cgaattttac tgctggagca aatgaactac
     5701 ctgccggaaa gcgaaagagt ttcttttgca gaaaaaggtg ggaatcaacg aaagggatta
     5761 tttcaaagag ccgttcaccc gcgatgaaat caaggcactg ctgcagggga aaccggcttc
     5821 cgacatgttc agttttaaat ccccgagttt taaggcgctg ggattgaatc ctgccgggct
     5881 ccacgacaag gatctgatga atctgatgtt gagggagccg cgactgatcc gccgtcccgt
     5941 cgtgaaaata ggaaggcatg tttattttgg cgcggacatc aaggtgctaa acgaaataat
     6001 cagacagtcc atagaaaacg gttgatcatt cctggtgatt gcaagtgccg aagaagacga
     6061 gcgcggggat agttttatat cgatttgtgg aagaggatat ggaagttttt cttgttcatc
     6121 ccggaggtcc tttctgggcg aaaaaagaca agggggcgtg gtctattccc aaagggctgt
     6181 ttgaaccggg ggaagatccg ctggaggccg cccggcggga attttatgaa gaaaccgggc
     6241 atgcggtgtc cggcgagttt agggcgttga tgcctttgaa acagcccagc ggaaaagtgg
     6301 ttcatgcctg ggtggtcgaa ggacatatcg atgaaaaatc cataaaaagc aatatgtttt
     6361 cgatggaatg gccgccccgt tcgggaaaag agcagtcctt cccggaagtg gaccgtggcg
     6421 gatggttcac actacgcatt gcctgtgaaa aaatattgcc gggtcagcgc ggtttccttg
     6481 acgagcttca aacaaagatt aaaaatcgtc ccgcctgagt gatgtctggc agcgaccatg
     6541 tggcggaaaa tgaattgact catgaataaa atatctgtac ctacacaaac gaaaaagacg
     6601 tcatcttctg tctgggtgca tcagaccggc gctttgttgt aaaggaaatt attatggatc
     6661 agaagataat ttgtctcagc gcggggttat cgcaggacga gattgaaaaa ggcaaggcgt
     6721 tgtattttgc cgccagtgaa ggccaatcga cgctggaggt gaccgccatg tcccggtcca
     6781 tgctgaatat gaaaatcatt gaaatactgg cgtgtctgat ggaggagctg gaaaaggatc
     6841 ccgcagagaa acaaaacgat tccggggacg ttattccggc ggatcattgt aagaccggag
     6901 tgcttgtcat gcttgcgcag gatcgtgagc tggtgttgca ggtgatgagg attttcaaag
     6961 cggtattgcc tgatccccgg aatgtgatct ttgcgatcat aacggagacg gcgctgaaat
     7021 ggacgctggc ggaatatctt gaacatctca gccgggaaca tgaatatatg cagacccgca
     7081 ggcccgaaaa taatccggat atgaaaaagt tgtaaagtgt tttgcaggag aaggaaacga
     7141 tgatttacaa tgaagaatac gagacgctgc cgcgcgaagc gctggaggca ttgcaattaa
     7201 agcggttaca gcaggttgtg aaccgggttt atcatcatgt cggtttttac aaaaaatcgt
     7261 ttgacaaggc aggcgtgcac cccaatgata tccgcacgct tgatgatctg aaaagatttc
     7321 ctctgaccac caaacaggat ctgcgcgaca attatccttt cggcatgttt gcgacgccga
     7381 tgagcaatat cgtccgtctg catgcttcat cgggaaccac gggcagttcc accgttgtcg
     7441 cctacacgaa gcgcgatatc gaaagctggg cggagctggt ggcgcgctgt tttgcggccg
     7501 ctgatctgac ccggggcgat atcattcata acgcttacgg ctacggtctt ttcacaggcg
     7561 gactcggcgt tcactacggt gcggaaaaac tcggcgccag cgtgattccc atgtctggcg
     7621 gcaataccaa gtggcagctg atgattctgc aggatttcgg tccgacggcg atctgctgta
     7681 caccttccta cgcgctgaat ctggcggagc agggccggac gatgggaatc gatatgcctt
     7741 cgctgaaatt gcgtgtgggc attttcggcg cagaaccgtg gagcgaacag atgcgtgccc
     7801 agatcgagga ggcgtttgcc atcaaggcca tgaatattta cgggctatcc gaaatcatgg
     7861 ggcccggcgt atcgatggaa tgcagcgagg cgcaggccgg tatgcatgtt tttgaagatt
     7921 atttcctggt ggaaaccatc aaaccggata cgggggaggt tctgcctccg ggtgaacagg
     7981 gagaactggt ttttaccaca ctgaccaagg aggcgttccc gctgatccgt taccggacgc
     8041 gcgatatttc ccgcctgatc acgacgccct gccgttgcgg acggacgttt caccgcatgg
     8101 aacgcgtcat ggggagaagc gatgacatgc tcatcatccg cggcgtcaat gtttatccgt
     8161 ctcagatcga atccgtgctg atcaacattg aagggttgga gccccactat cagctgatta
     8221 ttgaacgttc aggcacgctc gatacgcttg aagtgcaggt ggaagtgacg gaaggggtgt
     8281 ttgccgaggc cggcgaagtg aagggattgc agcaactgga gaaacgtctg atcaaggata
     8341 tgaaggacta tctgggcatc acggcaaagg tcaagctggt ggaaccgaaa agtatcatgc
     8401 gttcggaggg caaggccagc cgggtgattg ataaacggaa aatttaaaac agtcgtagga
     8461 catttcatta tattatataa tgctgttaat ggagggaaac gtttatggag ccgatcaaaa
     8521 tcatgccgtg tctggatatg aaaaacgggc gggttgtcaa aggagttcat tttatcgata
     8581 tcagggaagc gggcgatccg gttgaaaatg ccgaatttta tcaggctgaa ggcgccgatg
     8641 aactggccat gctggatatt gcagcgactc tggaaaaccg taaaacacgt ctggaatggg
     8701 tgaaaaatgt ttcgaaagtg atcaggattc ctctgaccgt cggcggcggc attgcttcgc
     8761 tggaggatat cgacatggtg ttgggtgcgg gcgccagcaa ggtttcgatg aacagcgcgg
     8821 cggtaaaaaa tccggaattg atccgtcagg cggcggaaaa attcggcaag gaaaaaatta
     8881 cagtagcgat tgacgcgaaa agaaacgctc agatgccgtc cggctttgaa ctggtggttt
     8941 ccggcggcac gatgccgatc gcccgcgatg ccgtggcctg ggcgaaaatc tgtcaggaaa
     9001 aaggcgccgg cgtgattctg cccaccagca tggacggcga cggcaccaaa gccggttacg
     9061 acatcccttt caccagagcg attgccgatg cggtgacgtt gcccgtggtg gcttccggcg
     9121 gggcgggcaa actggaagat ttctacgaag cggtggtcca gggcggtgcg acggtcctgc
     9181 tggcggcctc ggtgttccat ttccgtctgc tcaagattcg ggatgtgaaa acgtatctgc
     9241 gggaaaaggg tctggccatc gcctgatgag aaatgtcatg agcaaaaaaa ataacgccgg
     9301 gttcatgtat cttgctttgc ttggggcagc cataacaacg ctgtgtgacg gggtgcatgt
     9361 ttatacgcaa acgctttctt atacgacccc ctttcttttc ggtcaggcct ggtgggtttt
     9421 tccgggattt gtcctggcct ttctttttat gggcggggtc tatttggggg tagtgaatcg
     9481 tctcccgtta ttttttgctg tgaagaaaag catatccggg gggagtcttc atgatgtcgt
     9541 tgaaacggcc acggcctttg ccctgattta tgtgatgagc gggctgggca gcagtgaacc
     9601 gcttcttttg tcggcggttt tctacggaac ctttgtgttg cggctggcgt tcagttatga
     9661 ccggctattt ctgcttttac tcgcggttct gatggccctc ggcgggatgt ttgtcgaaag
     9721 cttgtatgcc gctgccggat tgatggcgta tcgttatccg gatgtgtttt atgtgccgtt
     9781 ctggctgggc ggactgtatg tacacggtgc ttttgccctg cgggagggca tgcgattttt
     9841 tgtctatggc agaaagccgg ggacgttaat ttccgtctcc cctttatgat gacagcgttt
     9901 catgcagtgt ccttcggtgc atccggccgg tggcatccga tgatggtaca tcatgatgtt
     9961 gttaaatccg gcggtgacga tcagctgaca atgagataaa atatagtggt gaagacgagt
    10021 gctggttgga aaacgatgat gaacgaagat aaaaaaacgt cttggcgaaa taagaaaagc
    10081 gatctgcgaa ggctcgttct tctgatgaac agacagaata acagattctg gccgccgctg
    10141 gatccgattc tcagaacggt tgatctggta attgagccgg aagaattgaa tctcctgctg
    10201 aaaatgggga ccgatttata cacttatgat caggttgcct tgctcagcgg catgaacagt
    10261 gaaaaattcg acgccgtctt cgactccctg aaaaagaaag cgtttatcgg aataaaaaat
    10321 cctgaaacgt ctggcgagcg gtataccctg catccgttcg tggtcggctg gtttgaaggc
    10381 caggtgtctt acctgatcgg caaacccgaa gaaaaagaat ttgccaaacg gtacatgggc
    10441 tttctggatt ctctgcgcaa atacaatttt ttccccctcc gcaatatcat gaatgtcatc
    10501 ggcaaacacg cgaccgtatc caatcaaagc gtcggaacag tgcgcgagtc cagaaacggt
    10561 cagggaaaat ccaaaattgt cgtggatgaa aacattgtcg taccggattc cggaatttat
    10621 ccgacaagct cggtaaatga tctgatcatg gaattcggaa ccagaagtat tatcggtcag
    10681 tttaaggttt gcatgtgccg gcagatgacc gcgaatgtcg atcaaccctg ccgcctgaaa
    10741 atgcccgatg atgttgcctg tatgggattt ggcgagggca tcaaaaccta catgaaatac
    10801 gcatatatgc gccagatcag taaagagcag gcttttgatg tcatccagaa agccagggat
    10861 cacggcgccg tccataccgt gtttcatgaa atggatgata cgaaactgcc gcagattggt
    10921 ttatgtaact gctgctggga ctgctgcggt attttccgta cgtataatat gggcgttgtc
    10981 cctttgcgtt accggtgtta ttatatagca agaatcgcaa acagcgaccg atgtacaggc
    11041 tgcggccgat gtgaaaagtt ctgtccgaca tccgcaatca acgtggtggc aaaaagcgtg
    11101 gtcctcgata caaaaaaatg catcggatgc ggccagtgcg ttcatcaatg cgttcatgcg
    11161 gtggttgaac tcgttgagga cagacgtacg gcctttttac ccatgctgaa aacatcggaa
    11221 gccaggctgc tgtaggaaaa gttgcgattc aagctcggaa acttcaggaa ggatgcgtta
    11281 tgccggattt aaagaaaaag caacacctgc cggatgattt gaaaaaatgc gtggaatttc
    11341 acgggcacat ctgtcccggc ctgatctacg gttatcgggt ggcccaagaa gcgatacgac
    11401 tgatgcatat cggacgctcg gtggatgagg aagttgtggc cgtctgtgaa aacgactcct
    11461 gtgccgtgga tgcccttcag gtgatgctgg gaacggtggc gggaaagggc aatctgttga
    11521 tcaaaaatta cgggaagaac gcctttacgg tgatgagccg agctgatcgc aaggcttacc
    11581 ggttttcccg caaaaaacaa tatgcttatg gcggctcttc cggacgccgg tttgaaaagc
    11641 tggaaagggc tgttgcatcc ggagacgcat cggaagagga gaaacaggaa ctgaaacgtc
    11701 tgaaaatcga cgatctgctg gcgcgtccct tttcggaaat ctttaccacg gaagaagtcg
    11761 ttttcgacgg acccggttat gcgccgctgg ctccttcgga gccctgtgct gtttgcggtg
    11821 aaatgaccat ggcaaccaaa atgattccgt tgcgcgacgg aaggcaagtt tgcattccct
    11881 gttcggagaa aagttaacat tgacatattc gggaagtatc gctaccgtcc tttgaaacag
    11941 ataaatcaat tcaaggagat tttctcatga aaaaaatagg cattacaatt ctggttgtgc
    12001 tgggagtctt aataacgtcg attgcttgcg cggccaatct ttatggcatt gtgtccgata
    12061 aaaacgggaa acctgttgag gtaaaagtga cgctcaagga cgacaaaggc gctgccgtgg
    12121 gtcagcctgt gacgacggat aaaaaaggag cctatgcgtt taaggatatc aagcccggtt
    12181 cgtatgtcgt ggtcgtcggt gcaaaggacg aatggaaaat attcgtggga cccggcgaaa
    12241 cgcggcgtga tttcagtttg aaataatttt caccggtgag gttttggaga atggctgaac
    12301 gcgacatcca gaaacaagaa gaaccgacgg aggctcccag gaagaaagac ctctgggata
    12361 tcctgcaggc actgggaccg gcgctgattg cggctgccgt agctgtcatc ggctcggtgt
    12421 acagctatca gcaggcccag atgaccaagg cttccgaaat gcgggaggtc tataccaaga
    12481 tcatgacgga gcgcgaaaat tcggacaata atattcgcgc cagtatgttc cagatgcttt
    12541 tcaatgcgat gttcgacaag gatatcaatt ccgcggacac gccggataat gtcaaccaga
    12601 tcaaaaaacg agtgatgttt ctggatgtgc tgtcacgcaa tttcgatact gtggacatca
    12661 aacctctttt cgaagatctg gatggagagt tgaccagaaa aatgtataat gaccgatcgt
    12721 attccgacgg cgcccaagaa gactattttg ccctgcggtc cgaattgcgg cgcatcggta
    12781 gaaatcttgc cgtcaaacag ctcaatgctc tggcatcctt acccggcacg atggtcaaaa
    12841 ccatcgttct gattgaagat aaggataaaa atatcatggt gatgcaggat gaaactacgg
    12901 cgaacggaaa cggtgatacg ctccccgtgg aaatcaaagc gcaaaatctt gatgacggca
    12961 aggtggaaat cgccatagat tatcagaaag ccaaagcttc cgataagggg ttcaaggcgc
    13021 cttcctttat tattaccttc tatgacatgc cttatatcga caacagcgtg ctcgacaagg
    13081 atatgcgcgt gggcgtggtg ctgaccaaat atgccacgac gagggaaatc gatttgttcc
    13141 gcgaccggat cgaccggcgt ctcctcaaag attacgaaga cctcaaagcc ggcggaattg
    13201 ccaaatatgc cgaactgcgg ttgatcaaat ttccggcgaa atacaccggc caccgcgatc
    13261 gaccttatct gcaggagatt ctccagaatc tcgtcgaaaa tgagaaatcc gtcagataaa
    13321 ccgggcgatc aatgatgtcg gatgaaagaa agaaacgata tacaacaaac aagtgtttcc
    13381 agatcccggg atctgatgaa aggaggataa tgtttatgaa aaaatatgtg ccagtcttgt
    13441 tgttgctgat ttttgttttt gcctgctggg cctgtgttcc cgtgcccgtt ccggtaggcg
    13501 gaacgacaac agtttatgac gaatcgtacg gatatgaaga gccgtatggg tatgatgggt
    13561 atgtagacgt tccggtcgtt tatggagaac cgatgtacta tgcgcctccg gtatccgtca
    13621 cgttcgcctt cgattacttt acgtatgaag ccatcggcgg atttgtggat gttgtgtttt
    13681 ggagaggcgg caaccgctat caccgggagc cgtggtatga acaaggaaga agaattacag
    13741 acagagatat acgctccggc aaacgatatc acaaagttcg cggaaaagaa ttttacaatc
    13801 atcgcgagaa actgcgcagc aagcacaaca ttgtccatcc cgattcgtat tacaaactgc
    13861 agcgctccaa acaaaaagag cagcagcgcc gtctggagga gcagaagaag cgggagctcg
    13921 aaatgcgcaa gcagagagaa aaacagcggc tggaagagga tcgcaacagg tataacaggg
    13981 aaaaacgcga gttgcaggaa aagcagcgcc gcctggagga acaaaagaag cgggaactg
//
LOCUS       DAOOLB010000053        13876 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4798_length_13876_cov_67.765791, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000053 DAOOLB010000000
VERSION     DAOOLB010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13876
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4798_length_13876_cov_67.765791"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PLF77_07480"
     CDS             <1..1268
                     /locus_tag="PLF77_07480"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC08732.1"
                     /translation="DRIAKLLSSLIEDGSTLHISPGFSEELVLEALGEKQNLGIHSLM
                     ILDVMKKLAEKGVITNRKKGFNDGKMVASGAIGGEELYRYLNGNPAVEFRPCDYVSNP
                     VMIARHHKMTAVNRVTSIDLKGQVAADGSAQNHFADVAGLVDFSRGAAMAPGGKSIVV
                     VPAASEDGLSSNIVLEQAAGAVAIPAADVTYVVTEYGAVNLFGKNVQERAMAMISVAH
                     PDFRERLFEEARREGLIGQERKLYEARYGVYPAWLEEALTIAGQKVTLRPVKTVDDRL
                     IQEHFYEMEEKDVAKRFFGRKHHFYWDEMKDMFIVDYAKNCSIVAYLGEEGYGRIIGI
                     GGYFLEGSGSGEVAYSVAKDWQGKGIAVRLQQKVVDAALANGLTGLDAVVLRENLSML
                     ALFKKLPYQIRTSFEEGILTLKCRFDEPV"
     gene            complement(1356..4580)
                     /locus_tag="PLF77_07485"
     CDS             complement(1356..4580)
                     /locus_tag="PLF77_07485"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HPC08733.1"
                     /translation="MPNFISEDQIEKAAVALLTNTYGYRTINCFTQEMEDLADRSNRA
                     SKQEVVLLDVLKAYAVKLNRDIPEDVIEEALDKLTSRRYAMSPLLANKEVYVLMRDGI
                     PVQYKDQNGKTEHGTVRVIDFAKPQNNDFCAVTQLWIKGERYPRRPDILIYVNGLPLV
                     FIELKNSNVKVQNAYDDNLTNYKKDIPLLFQYNAFCVLSNARETRVGSFTVGYEFFFS
                     WLRADDETEKINRKTIEQEASSLERVLSGLLPKDRLLDYIENFILYHRDTQKIVAQNH
                     QFLGVNKAIASFERRAGKNGKLGVFWHTQGSGKSFSMIFLARKVFHKYPGNYTFVIVT
                     DRDDLDGQIYRNFLDTNTVSKNEAARPGNSKEMREFLGRNKRLVFTLIQKFRFEKGKE
                     YPLLSDRNDIIVIVDEAHRTQYKDLAENMRKGIPNAQYFAFTGTPLLGRERKTNAWFG
                     DYVSEYNFIQSVDDGATVPLFYQKRVPEVLIQNDNLSDEFYSILEDENLDDKAQQKLE
                     REFSTELEVIKRDDRLETIARDIAAHFPQRGYLGKGMVISVDKFTCVKMYDKVQIHWK
                     VEIKRLVGVIGKIADGPYKERCKKQLDFMRSVEMAVVVSEDAGEEERFAKQKLDIKPH
                     RKKMNAIDENGHDIEYRFKAPDDKLQLVFVCAMWLTGFDAPTVSTLYLDKPMKDHTLM
                     QTIARANRVTSHTINGISKANGEVVDYYNVFRNMKKALSDYAMGDEQDETPVQDKTQL
                     FHLLGDALQQGLEFCQTLEVDLNKVLDAQDTFKRVNLFTAFADKLLEKDDHWKEFKVY
                     ENTISALYEACKPEILKKRFNPAIAAFQYLRGVVESVIGQADIEAVKQKIGELLDQSI
                     VTSNPELFAQSAQPQYAVIKKGKTLDLSTINFEKLKEEFHEKEFKHIEIANLRDFIEK
                     KLEVMLKENSTRTDFAQKLQEIINRYNAGGTATENYYDDLLKYAESLKEEDERHIREG
                     LTQDELELYDILKKDKMTKAEEIKVKNAARHLLQRLIKGRPKVLIQDWYKDGQSQHRV
                     KSAIEEVLDKDLPDTYEKDLFKEKSGKLYDLVYEYASKGQKWAA"
     gene            complement(4582..5460)
                     /locus_tag="PLF77_07490"
     CDS             complement(4582..5460)
                     /locus_tag="PLF77_07490"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPC08734.1"
                     /translation="MHSAVADMLKAYDCRTADDYRHALNEIVQELALLGLYRGGFFNH
                     AAFYGGTALRIFYGLDRFSEDLDFSLTKSNKKFDLSSFTQVVQDELGAYGLEMTVKEK
                     IKQNDSPVKSAFIKGGTQIHIVKIASIEQPVAGINPHEQLRIKLEVDTQPPPCATFEM
                     KYQLRPVPYSVRLYAPSSLFAGKIHALLCRSWKTRVKGRDFYDYVWFLSRSIPVNLAH
                     LAERMKQTGHLSAAAALTEKDLQGLLHKRFASVDFEQARKDVLPFIKNRDTVKLWSAD
                     FFSTITDDKLKIEKAK"
     gene            complement(5453..6055)
                     /locus_tag="PLF77_07495"
     CDS             complement(5453..6055)
                     /locus_tag="PLF77_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08735.1"
                     /translation="MPKNHLTLMHELQDYASPKARLTRMIKSGDVIQVRRGVFLDKRE
                     MSAYSLKSLAAIIYGPSYISFEYALSHYGLIPERVQAVTSAAYHKNKNRRFSTPLGDF
                     YYYYLPPAVFPYGIVLGSENEQGYLIATPEKALCDALYKTRGIESIKALEALLFEDWR
                     MEREAVLALDRPDIIFLAPRYRKKIFRLLLKWLGGEANHA"
     gene            complement(6104..7378)
                     /locus_tag="PLF77_07500"
     CDS             complement(6104..7378)
                     /locus_tag="PLF77_07500"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HPC08736.1"
                     /translation="MKKWNEYKLNDLVEFPPSVKLTRGEVYPFIEMEDVCPGKRYVSN
                     IQIRKYENSSCSKFTNNDVLLARITPCLENGKIAQVKLQSERSGFGSTEFFVFRGKKN
                     YIDQSFLYYLTKCDLIWKSAVNSMVGASGRQRADADFLKRLILNVPIMPIQRKIAAVL
                     SAYDDLIENNNRRIAILEKIAEELYREWFVRLRFPGHEKAKIVKGVPEGWKIKTVGKA
                     FEFCGGGTPSTSVNGYWNDGNINWYSPTDLTAANAVFSFSSKLQITEQGLKESSARLF
                     PAYSIMMTSRATIGQISINTTPACTNQGFITCIPNIQVPLYFLYYWLKLNKDYFIQIS
                     NGATFLELTKGKFKNVRIIIPCAQIICYYSKSQKLLFEQIELLLKQKDILTTTRDRLL
                     TRLMSGKIDVEHLDIQFPENMTEDAQHRDDET"
     gene            complement(7375..9408)
                     /locus_tag="PLF77_07505"
     CDS             complement(7375..9408)
                     /locus_tag="PLF77_07505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HPC08737.1"
                     /translation="MTPEQLKKLEATLWQSADTLRANSDLKSSEYSTPVLGLIFLKFA
                     DNKYGLYEQEIQAEYKKLKGTRRERSIDEIAIDKCGFYLPPEARYDYLLNLPEKMDIA
                     KALKNAMSLIEQFKPELKDTLPQDEYFRLVRKPEYKTIPKQLLKNFADIPKDVSGDMF
                     GQIYEYFLGEFAKTEGQKGGEFFTPRSVVRLMVQIIEPHNGTVFDPACGSGGMFVQSA
                     QFVEARKTPGEDASLFVYGQEKTLETVKLAKMNIAVNGLRGDIKQANTYYEDAHTSFG
                     KFDYVLANPPFNVDDVNLAKVEADRRFNTFGIPRNKTKAKDKGSVTVPNANYLWINLF
                     ATSLKPKGRAGLVMANSASDARHSEADIRKTLIEHNLIYAMLTLPSNMFYTVTLPATL
                     WFFDKDKPDDKILFIDARNIFTQIDRAHREFSEEQIQNIAVISRLRRGNRQAFVELVD
                     TYFQEGFTRLTENRPVLQDVSQKVVSFLKENNGKDLPDFVPVLKDTDKLLKAYAEYQK
                     AINVSLIDKANKAQRKLAETIAPFFTRLHELLKEVDKKVRHVEKNVGNGLKPFPTKAL
                     KTELDELHQDVKATEYFFDHIFWLQERFPEARYEDVTGLCKLASPKDVEEQEYSLNPG
                     RYVGVVIEEDGKTEEEFIEEMLSLNAELERLNREAGELEKVISHNIRQIAGEE"
     gene            9626..10291
                     /gene="radC"
                     /locus_tag="PLF77_07510"
     CDS             9626..10291
                     /gene="radC"
                     /locus_tag="PLF77_07510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPC08738.1"
                     /translation="MKTIKDLPEHSRPREKLQEKGATALTDEELVAAILGMGTAGRDV
                     RAIARQVALLIRDKQENLTLDDLMDVPGMGLAKSAQILSAFELARRHLLKETVKISRA
                     QDVLPLVSDLIDKRQEHFVCISLNGANEVIEKRIVTVGLLNMSPVHPREVFADVIVDR
                     AAAVIFVHNHPSGDLQPSDADMKMHEQLTEAGNILGLRVLDHIIISRRGYLSFQEVGI
                     IKN"
     gene            10374..11507
                     /locus_tag="PLF77_07515"
     CDS             10374..11507
                     /locus_tag="PLF77_07515"
                     /inference="COORDINATES: protein motif:HMM:NF017885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HPC08739.1"
                     /translation="MENKLNIKNIEQKVQELGLNQTTLAAKMQVSREAVSKWLKNEKY
                     PRPDKLLKLARLLNLSFSELVCKIPAANEPVIAFRKKGVHKITSDYIDQAKDMGMILQ
                     ELVPYLPFDDLSQPAVLKSPSVEYEYIRKVVGRVREKIGVGASEEIRFDHLIGFFNEL
                     QAVIIPVLWGNKENHENALHIYLPESMTTWIYLNLDCRIHDFKFWMAHELGHIHAPTL
                     QGEIGEDFAEAFAGALLVPHELAEREYKELQNLADIWSQIKRIKSVAEKLVVSPLTVY
                     YQINKYAAFCGKSEIDLETSKAIFQATTRFNQQFKPVNECLFNTKTPAPSRYVACAKE
                     LFGSPFFDVLKKYLTDYHKSAGFIQSLLNIPLLDAQNVYEELC"
     gene            11508..12071
                     /locus_tag="PLF77_07520"
     CDS             11508..12071
                     /locus_tag="PLF77_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08740.1"
                     /translation="MTQSRILVDSNAYFRLAQSIHPLLGVEFGAKRYCLYCLKELQQE
                     YNRSTRLQNTFSWVNDPEYVKNRSHLLKVTREEKLEISRANEFIYDYARNVYPGVSKV
                     DVLCLAHAEQLHIPVVTDDGDMSSVADAYGIKRIKTLELIRSMLDCRYISIGKIREIA
                     SYWIYMNDRPRDFAADYKKLFGEAAPK"
     gene            complement(12359..>13876)
                     /locus_tag="PLF77_07525"
     CDS             complement(12359..>13876)
                     /locus_tag="PLF77_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HPC08741.1"
                     /translation="PENEESGSTGVVDGLARIRGKWCVLIGFNNKWMAGAWIAGQPDN
                     NLRVTDIAKLLHLPLVWLVNCSGVKLPEQEKMYANRRGQGTCFFRHAELEQMGIPVIA
                     GIYGTNPAGGGYQSISPTILLAHKKANMAVGGGGIVSGMSPKGFFDEEGAEELINATR
                     HFKSVPPGSVKIHHDVTGFFRAVFDEETQVLDAIKDYMDDLPAYHPRFFRVAAPAEPA
                     YPPSEIASIIPVNKKRVYDFEQVLARLVDSSEHLEFRPGFGPEMYCGLVKVDGYLLGV
                     VGNRQGAFPNYPEYTNQYMGVGGKLYRQGLIKMSEFVTLCSRDKVPMAWFQDTSGIDV
                     GDIAEKAELLGLGQSLIYSIENSHLPMMLVVLRKGTAAAHYVLGGPTANNNNAFSLGT
                     ATTEINVMHGETAAAASYARRLVKEKDAGRPLGPVIDKMNEMVKHYDDTSGPLFCAKK
                     GFVDEIVRYDELRNYLVGFANCCYQNPRSICPQHQMILPRIIRSQMVKGLERPKA"
BASE COUNT         3226 a         3384 c         3600 g         3666 t
ORIGIN      
        1 cggaccggat cgccaagctc ttgtccagcc tgattgaaga cggctcgacg ctgcacatca
       61 gccccggttt ttccgaggag ctggttctgg aggccctggg ggaaaagcag aatctgggca
      121 tccactcgct gatgattctg gacgtcatga aaaagctcgc ggaaaaaggc gtcattacca
      181 accggaaaaa aggattcaac gacggcaaaa tggtggcttc gggcgccatc ggcggcgagg
      241 agctttaccg gtatctgaat gggaatcccg ctgtggagtt ccggccctgc gattacgtga
      301 gcaatcccgt catgatcgcc cgccaccaca agatgacggc cgtcaatcgc gtgacgtcga
      361 ttgatctcaa gggccaggtc gcggccgacg gatccgcgca gaaccatttt gccgatgtgg
      421 cggggctggt ggatttcagc cggggcgcgg cgatggcgcc gggcggcaag tccattgtcg
      481 ttgtcccggc cgcaagcgaa gacgggcttt ccagcaacat cgttctggag caggcggcgg
      541 gcgccgtcgc cattccggcg gcggacgtga cgtacgtggt caccgaatac ggcgccgtca
      601 acctgttcgg gaaaaacgtc caggagcgcg ccatggccat gatcagtgtg gcgcatccgg
      661 attttcgcga gcgtcttttt gaagaggccc gccgcgaggg gctgatcggc caggaacgca
      721 aattgtatga agcccggtat ggcgtgtatc cggcctggct ggaagaagcg ctgacgatcg
      781 ccggccagaa agtcaccctg cgtcccgtca aaaccgtgga cgaccggctc attcaggagc
      841 atttttacga aatggaagag aaggacgtgg ccaaacgctt cttcggtcgc aagcatcact
      901 tttactggga tgaaatgaaa gacatgttca tcgtcgatta cgcgaagaac tgttccatcg
      961 tcgcctatct gggcgaagag ggctacggcc ggatcatcgg catcggcgga tattttctgg
     1021 aaggaagcgg ctcgggcgaa gtggcctatt ccgtggcgaa agactggcag gggaagggca
     1081 tcgccgtgag gcttcagcaa aaagtggtgg acgccgctct ggccaacggc ctcacgggcc
     1141 tggacgccgt ggtgctcagg gaaaaccttt ccatgctggc gctgtttaaa aaactcccct
     1201 atcagatccg cacctctttc gaggaaggca tcttaacgct gaaatgccgg tttgacgagc
     1261 cggtgtgaac cctgatcatt gaacttcttg aatccgatcc tctttgggtg cgacgataca
     1321 gcatttccat agctacgttg agaggtcgtg gttttctaag cagcccactt ctggcctttg
     1381 gatgcatact catacacaag gtcataaagt ttgccgctct tttccttgaa taaatccttc
     1441 tcgtaggtat cgggcaaatc tttatccaga acctcttcaa tggcgctttt gacccggtgc
     1501 tggctctggc cgtctttata ccagtcctga atcaggactt tgggcctgcc tttgatcagg
     1561 cgctgcaaga ggtggcgggc ggcgttttta actttgattt cttccgcctt ggtcatcttg
     1621 tcttttttca aaatatcgta aagttccagt tcgtcctggg tgagcccttc ccggatatgg
     1681 cgttcatctt cctctttcag actttccgca tatttcaaga ggtcgtcata gtaattttcc
     1741 gtggccgtgc cgccggcgtt gtaacggttg atgatttcct gaagtttttg ggcaaagtcg
     1801 gtgcgggtgc tgttttcctt caacatcact tccagcttct tttcgatgaa gtcccgcaga
     1861 ttggcgattt caatatgctt gaattccttc tcatgaaatt cttccttaag cttctcgaaa
     1921 ttgatcgtgc ttaaatccag cgtttttcct tttttgatca ccgcatactg gggctgtgca
     1981 gattgcgcga acagctccgg attggaggtc acgatgctct ggtcgagcaa ctcgccgatt
     2041 ttttgcttga cggcttcaat atcggcctgt ccgatgacgc tttccacaac gccgcgcaga
     2101 tattgaaagg ccgcaatagc cgggttgaaa cgctttttga gaatttccgg tttgcaggct
     2161 tcgtacaggg cggagatcgt gttttcgtaa accttgaatt ctttccagtg atcgtctttt
     2221 tccagaagct tgtccgcaaa cgcggtgaag agattgactc ttttgaacgt atcttgagcg
     2281 tctaacactt tgttcaggtc aacttccagc gtttggcaaa attccaaacc ctgttgaagc
     2341 gcgtcaccca gcaagtggaa aagctgtgtc ttgtcctgaa ccggcgtttc gtcctgctcg
     2401 tcgcccatgg cgtagtccga caacgctttt ttcatgttgc ggaagacatt gtaataatcc
     2461 accacctcac cgttggcttt ggaaataccg ttgatcgtat gcgacgtcac acggttggcg
     2521 cgcgcgatcg tctgcatcag cgtgtggtct ttcatcggct tgtcgagata cagcgtggaa
     2581 accgtcggcg cgtcaaagcc cgtcagccac atggcgcaga caaacaccag ttgcagcttg
     2641 tcgtccggag ctttgaaacg gtactcgatg tcatggccgt tttcgtcaat ggcgttcatc
     2701 tttttgcggt gcggcttaat gtcgagtttt tgtttggcaa aacgctcttc ttcgcccgcg
     2761 tcttcgctca ccaccaccgc catttccacc gagcgcatga aatccagttg ctttttgcag
     2821 cgctctttgt aggggccatc ggctattttg ccgatcacgc cgaccagcct tttgatttcc
     2881 accttccagt ggatttgcac cttatcgtac attttcacgc aggtgaattt atccacggag
     2941 atgaccatgc ccttgcccag atagccgcgc tgcgggaaat gggccgcaat atccctggca
     3001 atggtttcca gccggtcgtc gcgcttgatt acttccagtt ccgtgctgaa ttcccgctcc
     3061 agcttttgct gtgctttgtc gtccagattt tcatcttcca gaatcgaata aaattcatcg
     3121 ctcaggttgt cgttttgaat caaaacctcc gggacgcgtt tctggtagaa aagcggcact
     3181 gttgcgccgt cgtcgacgga ctggatgaag ttgtattcgc tgacgtaatc gccgaaccag
     3241 gcgttggttt tgcgctcgcg tcccagaagc ggcgtgccgg tgaaggcgaa gtactgggcg
     3301 ttgggtatcc ctttgcgcat gttttccgcc aggtctttgt actgcgtccg atgggcttca
     3361 tcgacaataa cgatgatgtc gttgcgatcc gaaagcaggg ggtactcttt tcctttttca
     3421 aagcggaatt tctggatgag cgtgaagacc agccttttgt tgcgtcccag aaactcccgc
     3481 atttctttgg aatttccggg gcgcgccgcc tcgtttttgc tcacggtgtt cgtatccaga
     3541 aagttgcggt aaatctggcc gtcgaggtcg tcgcgatccg ttacgatgac aaaggtataa
     3601 ttgcccgggt atttgtgaaa caccttgcgg gccagaaaga tcatcgaaaa gcttttgccc
     3661 gatccctgcg tgtgccagaa aacgcccagc ttgccgtttt ttcctgcgcg gcgctcgaag
     3721 gacgcgatgg ccttattcac accgagaaac tgatgattct gggcgacaat tttctgtgtg
     3781 tcccgatgat aaagaataaa gttctcaatg taatccaaaa gccggtcttt gggcaaaagc
     3841 ccggagagca cacgttcgag actggaggct tcctgctcga tggttttgcg attgatcttt
     3901 tccgtttcat catctgcgcg cagccaactg aaaaagaact cgtatccgac ggtgaaactg
     3961 cccactcggg tttcccgcgc attggagagc acgcaaaacg cattgtactg gaacagcagg
     4021 gggatatctt ttttgtagtt ggtcagattg tcgtcatagg cgttttgcac tttgacattg
     4081 gagtttttca actcaataaa caccagcggc agaccgttga cataaattag aatgtccggc
     4141 cgccgggggt agcgttcgcc cttaatccag agctgcgtga cggcgcaaaa gtcgttgttt
     4201 tgcggtttgg caaaatcaat cactcgcacg gtgccgtgtt cggttttgcc gttttgatct
     4261 ttgtattgca ccgggatacc gtcgcgcatc agaacataaa cttctttgtt cgccaaaagc
     4321 ggcgacatgg catagcggcg ggacgtgagc ttatcaagcg cctcctcgat gacatcctcg
     4381 gggatatccc gattgagttt gacggcataa gctttgagca catcgagcaa aacgacttcc
     4441 tgtttggatg cgcggttgga ccggtcggcc aggtcttcca tctcctgtgt gaagcagttg
     4501 attgtgcgat agccataggt atttgtaaga agcgccaccg ccgccttttc aatctggtct
     4561 tcggagatga agttcggcat cctatttggc cttttcaatt ttcagtttgt cgtccgtaat
     4621 cgtagagaaa aaatcggcag accagagctt taccgtatcg cgatttttaa taaacggcag
     4681 cacgtctttt ctggcctgct caaaatccac cgaggcgaag cgtttgtgca agaggccttg
     4741 caaatctttt tcggttaacg ccgcggcagc agaaagatga ccggtctgct tcatacgttc
     4801 ggccagatgc gcgagattga cggggatgga gcgggataaa aaccagacat agtcgtaaaa
     4861 atctcgcccc ttgacgcgcg ttttccatga ccggcacagc agggcgtgaa ttttacccgc
     4921 aaaaagtgag gagggcgcat aaaggcgcac tgaatagggc acagggcgta actgatattt
     4981 catctcgaac gttgcgcaag gcggcggctg ggtatccact tccagtttga tccgcagttg
     5041 ttcatgagga ttgataccgg ccaccggctg ctcaatagaa gcgattttca caatatgaat
     5101 ttgtgtgccg cccttgataa aagccgattt gaccggggaa tcattttgct tgatcttttc
     5161 cttaaccgtc atctccagac catacgcacc cagctcatcc tgcacaacct gcgtaaagga
     5221 tgaaagatca aatttcttgt tgcttttcgt gagagaaaaa tccaggtctt cggaaaaacg
     5281 atccaaaccg taaaagatgc gcagcgcggt tccgccgtaa aaagcggcat gattaaaaaa
     5341 tccaccccga taaagcccca gcaacgcaag ttcctgaacg atttcgttga gcgcgtggcg
     5401 gtaatcgtcc gccgtgcggc aatcataagc tttgagcatg tcggcaacgg cgctatgcat
     5461 gattggcctc ccctcccagc cattttaaga gcagccggaa aatcttcttc cggtaacgtg
     5521 gcgccagaaa aataatatcc ggcctgtcca gagcaagcac ggcttcgcgc tccatccgcc
     5581 agtcctcaaa aagcaacgct tccagggctt tgatgctttc gattcccctg gtcttgtaaa
     5641 gcgcgtcgca aagcgccttt tccggcgtgg cgatcaggta gccctgctcg ttttccgaac
     5701 ccagcacaat gccataggga aagaccgccg gcggcagata gtagtaatag aaatcgccca
     5761 gcggcgttga aaagcggcgg ttcttgtttt tatggtaagc cgccgatgtg acggcctgca
     5821 cccgctccgg aatcagtccg taatgagaca gggcgtattc aaaggatata taggacggtc
     5881 cgtaaatgat cgccgcaaga cttttgaggg aataagcgga catttcgcgc ttgtccagaa
     5941 agactccccg gcgcacctga atcacgtcgc cggacttgat catccgggtc aaacgggcct
     6001 tgggcgaggc atagtcttgc agttcgtgca tcaatgtcag atgattcttt ggcatattgt
     6061 ctccagtaag tatcaattta ttgtactttt tggagaaagt caatcatgtt tcatcatccc
     6121 ggtgttgcgc gtcctccgtc atattctcgg gaaattgtat atccagatgt tccacatcga
     6181 tcttgccgga catcaggcgt gtcaggagac ggtcgcgggt agttgtaagt atatcttttt
     6241 gttttaaaag tagttctatt tgctcaaaga gaagtttttg acttttggaa taataacaga
     6301 taatttgcgc gcacggaatg attattctta cgttcttaaa ttttcccttt gtcaattcta
     6361 aaaaagtagc tccatttgaa atttgaataa aataatcctt gttaagtttg agccagtaat
     6421 agagaaaata taaaggaact tgaatattag gaatacaagt aatgaaaccc tgattagtac
     6481 aagcaggtgt tgtgtttatg cttatctgcc caattgtcgc cctgcttgtc atcatgatag
     6541 aataagccgg aaatagacgg gcagaacttt cttttaagcc ttgttcagtt atttgtaatt
     6601 ttgatgagaa tgaaaatact gcattggctg ccgtcaaatc tgtaggtgag taccagttaa
     6661 tatttccgtc attccaataa ccatttactg atgttgacgg tgtacctcct ccacaaaatt
     6721 caaaggcttt tcccactgtt ttaatcttcc atccttccgg tacacctttg acgattttgg
     6781 ccttttcatg gccggggaag cgcaggcgga cgaaccattc acggtagagt tcttcggcaa
     6841 tcttttccag aatggcaatg cggcggttgt tgttttcaat caaatcgtca taagccgaaa
     6901 gcaccgccgc aatcttacgc tgaatgggca tgatgggaac attaagaatt agtcttttaa
     6961 ggaagtcggc atcagctctt tgtcgtccac ttgcgcctac catactgttg acagcacttt
     7021 tccagatcaa atcacatttt gttaaataat ataagaaact ctgatcaatg taatttttct
     7081 ttcctctaaa aacgaaaaat tcagttgatc caaagcccga gcgttcgctc tgtaatttaa
     7141 cttgtgcaat tttaccattt tcaagacaag gtgttatcct agctaagaga acatcattat
     7201 ttgtaaattt tgagcaacta ctgttttcat attttctaat ttgaatatta gaaacatatc
     7261 ttttaccagg gcaaacatct tccatttcaa taaatggata aacttcacct cgggtcagtt
     7321 taacagaagg tggaaattca actagatcat taagtttata ttcattccat ttcttcattc
     7381 ttcccccgca atctgacgga tattgtgtga gataactttt tccaactcac ccgcttcgcg
     7441 attaagcctt tccagttccg cgtttagcga aagcatctcc tcaatgaatt cttcctctgt
     7501 cttaccgtct tcttcaatga cgacgccgac ataacggccg gggttgaggg aatattcctg
     7561 ttcttcaacg tctttcggag atgcaagctt gcaaaggccg gtgacgtctt cataccgcgc
     7621 ttcggggaaa cgttcctgta gccagaagat gtgatcaaag aaatactcgg tggccttgac
     7681 gtcttgatga agctcatcca gctcggtctt gagcgctttc gtagggaacg gtttcaaacc
     7741 gttccctaca tttttttcaa catggcggac ttttttgtcc acttctttca aaagctcatg
     7801 cagtctggtg aaaaaaggcg ctatcgtttc ggccagcttg cgctgggctt tgttggcttt
     7861 gtcgatgagt gaaacattta ttgctttctg gtattcagca taggctttca gcagtttatc
     7921 ggtgtctttg agtacgggta caaaatccgg caaatccttg ccgttgtttt ctttcaaaaa
     7981 gctgaccacc ttttgcgaaa cgtcctggag gacagggcgg ttctcggtaa gcctggtaaa
     8041 cccttcctgg aaataggtgt ccaccagttc cacaaaggcc tgacgattgc cccggcgcag
     8101 gcgactgatg acggcaatgt tttgaatctg ctcttcggaa aactcccgat gcgcgcggtc
     8161 aatctgcgtg aagatatttc gggcgtcgat gaagagaatc ttgtcgtccg gtttgtcttt
     8221 gtcaaaaaac cacagcgtgg ccggaagcgt cacggtgtaa aacatgttcg acggcagcgt
     8281 gagcatggcg tagatgaggt tgtgttcaat gagcgttttg cggatgtccg cttcgctgtg
     8341 acgggcgtcg gacgcggaat tggccatgac cagcccggcc cggccttttg gcttgagcga
     8401 cgtggcaaaa aggttgatcc agaggtaatt ggcattgggg acggtgacag agcctttgtc
     8461 tttggctttg gtcttgtttc ggggaatgcc gaatgtgtta aaacgccggt cggcttccac
     8521 tttggcaagg ttgacgtcat ccacattgaa aggaggattg gcaagcacgt aatcgaattt
     8581 tccgaagctt gtgtgagcgt cctcgtaata ggtgttggcc tgcttgatat cgccgcgcaa
     8641 accattgacg gcaatgttca ttttggcaag tttgacagtt tcgagcgttt tttcctgccc
     8701 gtaaacaaag aggctcgcat cctcgccggg cgttttacgc gcttcgacaa actgtgcgga
     8761 ctgcacgaac atgccgccag agccgcaggc gggatcaaag acagtgccgt tgtgcggttc
     8821 gattatttgc accatcaggc ggaccaccga acgcggcgta aaaaattctc cgcctttctg
     8881 gccttccgtc ttggcaaatt cgccgagaaa gtattcgtaa atctggccga acatgtcgcc
     8941 tgagacgtcc ttggggatgt cagcaaagtt tttgagcagt tgcttgggga tggttttgta
     9001 ctccggtttg cgcaccagac gaaagtattc atcctgtggc agagtgtctt tgagttccgg
     9061 cttgaattgc tcgatcagag acatggcgtt ttttaaagct ttggcgatgt ccattttttc
     9121 aggcaaattc aacaggtaat cgtaacgcgc ctccggcggc agatagaagc cgcacttgtc
     9181 gatggcgatt tcatcgatgg agcgctccct gcgagtgcct ttgagctttt tgtattccgc
     9241 ttgaatttcc tgctcgtaaa gtccgtattt gttgtcggca aatttcagaa agatcagtcc
     9301 aagcaccggt gtggagtatt cggatgattt gagatcggag ttggcacgaa gagtatcggc
     9361 ggactgccag agcgttgctt ccagtttttt gagttgttca ggcgtcatga atgtatccag
     9421 tattcagttt ttgattttga aaatgaacgc aaaagatctg ttgtttgttg cggtgaagta
     9481 taggttcatg ctccggccgt gtcaagaaaa atgatacctt tatcatgaat atctaattga
     9541 cattactggg ggaataagct tttatggatt cgtcttcgat tccatttttg attattgcat
     9601 catcgttcgt catgggaggg aacctatgaa aacgatcaaa gacctgccgg agcacagccg
     9661 tcctcgcgaa aagttgcagg aaaagggcgc cacggcgctg actgacgagg aactggtggc
     9721 ggcgatttta ggcatgggga cggccgggcg tgatgtacgc gcaatcgcca ggcaagtggc
     9781 attgctgatc cgtgacaaac aagaaaatct tacccttgat gatttaatgg atgtgccggg
     9841 catggggctt gccaaatcgg cacagattct ttccgctttt gaactggcgc gcagacatct
     9901 gcttaaggaa acggtcaaaa tcagccgcgc ccaggatgtc ttgccgctgg tttccgactt
     9961 aattgataaa cgacaggagc attttgtctg tatctcgctc aacggcgcga atgaagtgat
    10021 tgaaaaaagg atcgtcaccg tcggcctttt gaacatgagc ccggtgcatc cgcgcgaggt
    10081 ctttgctgat gtcattgtgg accgggcagc ggcggtgatc tttgtgcata atcatccttc
    10141 gggcgactta cagcccagcg atgcggacat gaagatgcat gagcagttaa ccgaagccgg
    10201 aaacattttg ggcctgcgtg ttcttgatca tatcattatt tcccgccggg gttatctgag
    10261 ctttcaggaa gtggggataa tcaagaatta aggaaacaag agtacaatca acaacttctc
    10321 tcgttatgtc aataaaagtt gacaaaagtt gactaagtgt gcaatgtaga accatggaaa
    10381 acaaactgaa tatcaaaaat atcgagcaaa aagttcagga gctaggcctg aatcaaacaa
    10441 cgcttgcggc aaaaatgcag gtgtctaggg aagctgtatc caaatggctg aaaaatgaaa
    10501 aatatccccg cccggacaag cttttaaaac tggcgcggct gctgaatctc agtttcagcg
    10561 aactggtatg caaaattcct gcggcgaatg aaccggtgat tgccttccgt aagaaaggcg
    10621 tccataaaat tacatcggat tacattgatc aggctaaaga catgggcatg atactacaag
    10681 aattagtgcc ctatctccca tttgatgatc tttcccagcc tgccgttctg aagtcgcctt
    10741 ctgttgagta tgaatacatc cgcaaagtag tcggccgggt ccgggagaaa atcggtgttg
    10801 gcgcaagcga agaaatccgc tttgaccatt tgatcggctt cttcaatgaa ttgcaggccg
    10861 tgattattcc cgttctctgg ggcaacaagg aaaatcatga aaatgcgctg catatttatc
    10921 tgcccgaatc tatgaccact tggatttacc tgaatcttga ttgccgtatt catgatttta
    10981 aattctggat ggcccatgaa ttggggcaca ttcatgcccc aacgctgcag ggagagatcg
    11041 gcgaagactt tgccgaagct ttcgcggggg ctctgctggt tccgcatgag ttggcagagc
    11101 gcgagtacaa ggagttacag aatcttgccg atatttggtc acagatcaag aggatcaaat
    11161 cagttgcgga aaaactggtt gtttcaccgt tgacggttta ttatcaaatc aataaatatg
    11221 ctgcattttg cgggaaatcc gaaattgatt tggaaacgag caaggcgatt tttcaggcta
    11281 cgaccagatt caatcagcaa tttaagccgg tcaatgagtg tttgtttaac acaaagacgc
    11341 ctgcgccatc ccgatatgtg gcttgtgcga aagagctgtt cggaagccct ttctttgatg
    11401 ttttgaagaa atatctgaca gactatcaca aatcggcagg ctttattcag agcctcctca
    11461 acataccgct ccttgatgca caaaacgtgt atgaggagct ctgttaaatg acacaaagcc
    11521 gaattcttgt agattccaat gcctatttcc ggttggctca aagcattcac ccgcttttag
    11581 gtgtggagtt cggcgctaag cgatattgcc tttactgtct caaagaactg caacaagaat
    11641 ataatcggag cacacgcctg caaaacactt tttcatgggt gaatgatccg gaatacgtta
    11701 aaaatagatc tcatttactg aaggtgacca gagaagaaaa gctggaaatc agtcgagcga
    11761 atgaattcat atacgactat gcacgcaatg tgtaccccgg tgtatccaaa gtggatgttc
    11821 tttgtcttgc ccatgcggag caattgcaca ttcctgttgt cacggatgat ggcgatatga
    11881 gcagcgtggc agatgcgtat ggaattaaaa gaatcaagac gcttgaactt ataagatcga
    11941 tgctggactg tcgatatatt agtattggca aaatcagaga aattgcctcc tactggattt
    12001 acatgaatga ccggcctaga gattttgctg ctgattataa aaagcttttt ggcgaagcag
    12061 cgccgaaata atcattttgc tttgcaggcg gcagatttgt tttctaaggc aagaccattc
    12121 atattccatg acaaatacca tatggtatct gaataagtca ttgatattga gtagatggaa
    12181 ggtatttttt gattcaaata ctgcgacatt taagcggctt atttgaaatt gtaagtggtc
    12241 gatccttgca ttggattcaa taaaaaggcg gggtgtttcc cgccttctat cctgaatatg
    12301 gggtacacag agtgtaagga gcggtcgcgc cacacgaaag ggcggttcaa accgttcctt
    12361 aggctttggg cctttccagc ccctttacca tctgcgagcg gatgatgcgc gggaggatca
    12421 tctggtgctg cgggcagatg gaccggggat tctgatagca gcagttggca aagcccacca
    12481 ggtagttgcg cagctcgtcg tagcgcacga tttcatcgac aaagcctttc ttcgcgcaga
    12541 acagcggccc cgacgtatcg tcatagtgct tcaccatctc gttcatcttg tcgatgacgg
    12601 gcccaagcgg ccggcccgcg tccttttcct tgacgagcct tctggcgtaa ctggccgccg
    12661 cggcggtttc cccgtgcatg acgttaatct cggtcgtggc cgtgccgagt gaaaaggcat
    12721 tgttattgtt ggccgtggga ccgcccagaa cgtaatgcgc cgccgctgtt cccttgcgca
    12781 ggacaaccag catcatcggc aggtgcgagt tttcaatcga atagatgagc gactggccca
    12841 ggcccagaag ctccgccttc tccgcgatgt cgcccacgtc gatgccggac gtgtcctgaa
    12901 accaggccat cggcaccttg tcgcgcgagc acagcgtgac gaactcactc atcttgatca
    12961 gtccctggcg gtagagcttg ccgcccacgc ccatgtactg attggtgtat tccgggtaat
    13021 tgggaaaggc gccctggcgg ttcccgacga cgcccagcag ataaccgtcc accttgacca
    13081 ggccgcagta catttccggg ccgaagccgg ggcggaattc cagatgctcc gagctgtcca
    13141 cgagcctcgc cagaacctgc tcgaaatcgt aaacgcgctt tttgttgacc ggaatgatcg
    13201 aggcgatttc cgagggaggg taagcaggtt cagcgggggc tgccacgcga aagaagcgtg
    13261 gatggtaggc gggcagatcg tccatgtaat ctttaattgc gtccaagacc tgggtttcct
    13321 cgtcgaagac cgcacggaaa aatcccgtga cgtcgtggtg gattttgacg ctgccgggcg
    13381 gaacggactt gaagtgccgc gtggcgttga tgagctcttc cgcgccttct tcatcgaaaa
    13441 atcccttggg ggacatgccg ctcacgatgc cgccgccgcc cacggccatg ttggccttct
    13501 tgtgcgcgag cagaatcgtc gggctgatgg actggtagcc gccgcccgcg ggattggtgc
    13561 cgtaaatgcc ggcgatgacc gggatgccca tctgttccag ttcggcatgg cggaagaagc
    13621 acgtgccctg gccccggcgg ttggcgtaca tcttttcctg ctcgggcagc ttcaccccgg
    13681 aacagttcac cagccacacc agcggcaggt gcaggagctt ggcgatgtcg gttacacgca
    13741 ggttgttgtc cggctggccg gcgatccacg cgcccgccat ccatttgttg ttgaagccga
    13801 tgaggacgca ccacttgcct cggatgcgcg ccagaccatc gaccacgccg gtgctccccg
    13861 attcctcgtt ttccgg
//
LOCUS       DAOOLB010000054        13825 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4814_length_13825_cov_52.661583, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000054 DAOOLB010000000
VERSION     DAOOLB010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13825
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4814_length_13825_cov_52.661583"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..1417
                     /locus_tag="PLF77_07530"
     CDS             380..1417
                     /locus_tag="PLF77_07530"
                     /inference="COORDINATES: protein motif:HMM:NF027495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HPC08742.1"
                     /translation="MTHTELTSGGKPFVHHHAAHCETGVTAALFRDKGLEISEPMIFG
                     IGSGIFFGHLPFVKFVGLPISTYRAQPGAVFRKAAKRLDGKFVTKKYTNAKKGMDELL
                     RVLRSGQIVGLTTNIYWLSYFPDRFRFQFNGHNIVILHEIENGFRVSDPVLDKPVDCR
                     TEDLIRARFARGPLEPHGFMYYPVSVNLHPDLRGACIKGMLDTCNMMLRIPVPIFGIR
                     GIRFLAKRIIKFEKTQGFEEACRQLGMIVRMQEEVGTGGAGFRYMYAAFLQEAADLFG
                     SAELNDLSQDMTAIGDTWREFAVTAARIIKKRGKQDETFAKAGGLMLICAGREENLFK
                     NLHEIAGKLKS"
     gene            1414..2160
                     /locus_tag="PLF77_07535"
     CDS             1414..2160
                     /locus_tag="PLF77_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC08743.1"
                     /translation="MKALDIAQVTFSYNPKEGKIKTAGAEYALRDFSMSVDSGQMHAL
                     LGPNGAGKTTLMRLITGQLRGFQGSISIYGTRNDHAQSLSSVGYAPQPISVYAGLTAM
                     ENMRVFGVMAGLSRELLQERSRAVLEETGLLPYAQKRASTYSGGMLRRLNLAVALLHA
                     PRLLLLDEPTVGVDPQSRNHIYETLKKMNASGIAIVLCTHIMDEAQRMCSKVTMLDRG
                     AVIFSGAMNEIDHLETFFLAQTGHRLRDED"
     gene            2176..3297
                     /locus_tag="PLF77_07540"
     CDS             2176..3297
                     /locus_tag="PLF77_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC08744.1"
                     /translation="MTFRKLTALIRKEMIILLRERQTIPLLFIMPMALIFFLSLALQG
                     VYMDKLTGRQILLIIENESHQPTANLLEQRISANKMIQRVQRPPGFDNDKVFETSRAH
                     AVIFIPKGFDEGLKPVEMEFDPVLDAGYKSAVRVLVTGLTVEVVMGIDDIEGMSADFI
                     VEKTKTNRTLPGPLQNSVPGWSIFAMFFIAIPMSIGFLREKNDGTLQRIFTYPVSPAI
                     VILGKLIPYYLINLMQFALMLIVGIYILPLIIAFPFTLGEHPWHVIPVTLVVAAASTG
                     FGVMVAALARAPEQSSALAATGAVLMAVFGGIMVPHAVMPLIMKKLAMLSPMYWAHQA
                     YLDIFSRDAGFVSIWPKLAVLTVFALVCFYIAGRKVKWM"
     gene            3288..3599
                     /locus_tag="PLF77_07545"
     CDS             3288..3599
                     /locus_tag="PLF77_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08745.1"
                     /translation="MDVTQKLKDLNVSSREELIYKLKELVIRACEITDVRSEDVPTDV
                     PFIGGPGPLLLDSLDAMEIAMEIRFQFGVELKNASTAAKAMQNFDTLADFIIEAPRVK
                     K"
     gene            3596..4792
                     /locus_tag="PLF77_07550"
     CDS             3596..4792
                     /locus_tag="PLF77_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPC08746.1"
                     /translation="MNSGDIYVRGWGAVTTLGWNATDAARNLIEAQAPPAKPGRSSHL
                     INRDFNVFEVSHPGNPLEKAMAMLEGAIDEAFERAGLSPDEIAESSVLIGTTGGLFIR
                     NEHEFIEAVRKNPDGETPPISCRNRGAGEVADFITKKYGIRGMALTFSIACVSSSHAL
                     VVAADLLQSRKIRRAIVIGFEPLLNMTLHGFSSLLLFDYERCRPFCATRAGMQIGEAA
                     SAVILDSGQNLSSRYTFSGGYLYNDIKSMTSAGIDGVTVSEVVRHTLDSARLSADDII
                     AIKAHGTGTKDNDLSEGRGLVRLFGEKLPPVVSLKGAIGHTLGAAGTAETALWLSCLD
                     EGIIPRSFGFSKMDPEIGFTPSQKNIDARDGAYLFTFFGFGGTCTSYLIIRNSTENSS
                     ARAGSS"
     gene            4835..5824
                     /locus_tag="PLF77_07555"
     CDS             4835..5824
                     /locus_tag="PLF77_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08747.1"
                     /translation="MTETTTKHTAPASPIPVIAASYIGPPLMPNRDALWEENELPLNA
                     DALVNEVMGQPMRRAGRFIKMVSCGGLACLQQVSPDYLKGKKTGIFLATGLGNVDEIL
                     PFITQVFKHGGGFPSPNQFANSVSNAAAFVMARICEVTGTVLTVSQEELSFESALWLA
                     QTYLEHGRIDIALVGGCDIFTPTAEEYRERVNVSCGNARRLPMGESSSWLLLGKNEEQ
                     NIGHILAVDFYDTVGAPDQRFDKQTILSDLCDARGKGRIVLPGFRIRQTDLNGWKDGG
                     WETEDYLPCCGIHSTASAFGIARTLREKTNSVFVHYNCSASGRHAFVSGSTKS"
     gene            5821..7278
                     /locus_tag="PLF77_07560"
     CDS             5821..7278
                     /locus_tag="PLF77_07560"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08748.1"
                     /translation="MMVSSRQKKRIVLVHPRGFNWFPGKRDITDIANRMVPQGVLSIA
                     AYLKQKGHEVFVYDCLGPGAPLDLQKQAGAILAYQPQMVGFSATTSSFPDAADLARLI
                     KNHSSAIATVCGGVHVSALEGKLLNAYPAFDFLIAGEGEETIAELADEIPAADIRGLI
                     WRNQDEITVNDVRPKISDLDSLPFPAYEQLKGFPRDYHLPLFSYIHTPGATMITSRGC
                     LYQCSYCDRSVFKKGFRYNSAGYIYDHMKYLRETFGVRHINIYDDLFTANRPRIVKLC
                     EKLSRRPLGLHFNCAVRIGYTDDDLLDMLKDAGCLMVSLGIESADPAMLARHKSGINL
                     DDVRDTVKRIQKAGLRAKGLFMMGLPGETEASVKRTSDFILSLDLDDMNMAKFTPFPG
                     APLWTAIREEGTFHEDWRLMNCLNFVFVPRGIASKERLDQLYNEYVKRFYSDKKWRKK
                     FRNRIWQHRNSLLYLIRHLPSFWSAKNQFEPQKGK"
     gene            complement(7529..>8074)
                     /locus_tag="PLF77_07565"
     CDS             complement(7529..>8074)
                     /locus_tag="PLF77_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPC08749.1"
                     /translation="IVGLLLNKGADVNLKTKFIGATALILTAAKGHADVARLLIDKGA
                     DMNAKNKDGITVLMVASLNGHRAVVQLLLDKGVPVNGKSNENITALLMASERGHKDII
                     QILLDKGADVNAKDSDGLPPVLLAAQKGHKDVVTLLLDKGADGNVRAKIKDTEYTLLT
                     AAEINKWTDIVERLKKSGVKE"
     assembly_gap    8075..8174
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8175..9009)
                     /locus_tag="PLF77_07570"
     CDS             complement(<8175..9009)
                     /locus_tag="PLF77_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HPC08750.1"
                     /translation="MFWKKIKNAVCPVKHILRVPVGLFIVALVCADAGAAQNNDRLIQ
                     AAEKGLLQDVQAALAQGADTNAKSVKKYYNIFGKEITPLIAASENGHAEIVKLLLQKG
                     ADPNFQRLPERTTALMLAAENGHADVMKILLEGKADINAINNTGITALMLASKNGHPD
                     AVGILMQGKVDVNTRNNDGVTALMLAAQYGHHDIVQRLVQHQADVKIKNNDGVTALML
                     ASKNGHAKVVERLLETDAGVNVQRASDNATALMMAAENNHREIVGLLLNKGADVNLKT
                     KF"
     gene            9192..10523
                     /gene="xseA"
                     /locus_tag="PLF77_07575"
     CDS             9192..10523
                     /gene="xseA"
                     /locus_tag="PLF77_07575"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPC08751.1"
                     /translation="MEQILTVSELNNTIKSLLEESFDSLLVEGEVADLRRPQSGHTYF
                     TLKDDKSQIRAVFFRPFGRTGGRVNFELEEGLKILCRARLTLYPPRGEYQLVVESVEP
                     LGVGALQKAFEQLKAKLSAEGLFDKQYKKALPFLPERIGVITSPTGAVIRDILNVSRR
                     RFPATDILIAPVRVQGNDAANDMIQALRRFHAFGQVDVIILARGGGSLEDLASFNDER
                     LAREIFRSDIPVVSAVGHETDFTICDFAADLRAPTPSAAAELVLPDRTELIFKVNMLA
                     RRMTAGCLRHLKAEKEHLEGLQERFKDPRLAVVTQKIRIDDLKESLSAAFGHRRQILL
                     HKLNHLELRLKHQSPEQQINEKQIVLKNLRKELHSSLSCVLTALKERFQKDAAVLESL
                     SPLAVLERGYSLTRTVPEGTLIRQTRDLHEGDEVNVLLAKGNFNAKIQKIF"
     gene            10646..10885
                     /locus_tag="PLF77_07580"
     CDS             10646..10885
                     /locus_tag="PLF77_07580"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPC08752.1"
                     /translation="MMAKEKFEDALEKLEDIVKKMEAGDMPLDETLKSFEEGIKLIRF
                     CSAKLEETERRVELLLEKENTLQVKRFQGDDHDAG"
     gene            10872..11774
                     /locus_tag="PLF77_07585"
     CDS             10872..11774
                     /locus_tag="PLF77_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPC08753.1"
                     /translation="MMQDDDLFLKAYLEDRQNIVEEALQRYLPEDGFPSDIYKAVHYS
                     VFNGGKRIRPILCLAAAEAVGGDLVPAIPVACALELIHSYSLIHDDLPSMDNDDFRRG
                     KPTCHKVFGENMAILAGDALLTEAFVLLSRAEKVMLSAERRLAIIQEIANAAGINGMV
                     GGQALDVISEKTGADETILQDIHRRKTGALIVAAVKSGAVMFNAGKDKIQALTEYGMH
                     VGLAFQIADDILNVEGDSRLMGKGTGSDAARNKMTYPSLMGLQQAKEKLNQHVEAAVT
                     SLADFDERAMPLIVIARYIRDRKS"
     gene            11793..13715
                     /gene="dxs"
                     /locus_tag="PLF77_07590"
     CDS             11793..13715
                     /gene="dxs"
                     /locus_tag="PLF77_07590"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HPC08754.1"
                     /translation="MVKQPQTVHYSVLKDVNFPADIRGLTLAQLNTLAQEIRDLITLT
                     VASCGGHLAASLGTVELTLALHYAFNTAQDKIIWDVGHQSYAHKIITGRKEDFKNLRK
                     QNGISGFPRREESPYDVFNVGHSGTSISAAAGMTEAACLKGASYKIIAVIGDGSMTSG
                     MAFEGLNWVGDRKKNMIIVLNDNEMSISENVGAMSSYLNRVMTGHKITTLKADIKRFL
                     KSIPGVGGDMIKFAQKIEEILKTLMVPGALFEEMGFTYVGPLEGHRLDHLIKTFENIR
                     EMTGPVLVHVITKKGKGYSFAEQNPPSYHGVAPFDVQTGQVIASSSGIPTYTNVFGRT
                     LVKLARTDSRIVAITAAMREGTGLNLFSQEFPNRFYDVGIAEQHAVTFAAGLATEGFI
                     PVVAIYSTFLQRAYDQIVHDVCLQKLPIIFAVDRGGLVGEDGATHQGLLDYSYLRSIP
                     HIVVMAPKDENELQHMLKTAVDCGQPVSLRYPRGSGAGRPLDEVLTALDIGKGEVLRG
                     GTDLAVMAIGSTVLPALAAANRLAEEGRDISVINARFVKPLDEELILKTAAAVGTIIT
                     VEENTLQGGFGSAVLELLAEKSITGIRVKRLGIPDEFVRHATQAQQRRRYGIDEEGIM
                     AAINEILTSESFRRPS"
BASE COUNT         3230 a         3679 c         3624 g         3192 t
ORIGIN      
        1 gaccgccggc attgccgtgt ttcgcgtcaa gcggcatctg cggccggaaa ttttttccag
       61 gctgaaaaat ccggtattgg aacgctatgc gaaagctctt gcaatcacga tggaagaatt
      121 aaaaaccgtg cctgaggaat cgcgggaata aatcctgtag catgcggcac ggtgcctcta
      181 agcgtcgtta gtgtgataat ttccaatccc gattcatggg gattggataa ttcggctggc
      241 aaatttcaat acgctttgct tttgaaattt gaaactcatt acaattacca attccattgc
      301 gcctatagcg gcctttaggt tattcgtttt cggaattggc gtttcatgaa aacctgggac
      361 tcattagaag gacttattat tgactcatac agaactgaca tccggcggga aaccgtttgt
      421 tcaccatcat gccgcccact gtgaaaccgg cgtgacggcg gcgctattcc gggataaggg
      481 attggaaatc agcgagccga tgattttcgg catcggcagc ggcatctttt tcggccatct
      541 gccgtttgtt aaatttgtcg gcctgccgat cagcacatat cgggcgcaac cgggtgcggt
      601 gtttcgcaaa gccgccaaaa ggctcgatgg aaaatttgtc acaaaaaaat acaccaacgc
      661 aaaaaaaggc atggacgaac tgctccgtgt gctgcgcagc ggccagattg tcggactgac
      721 caccaatatt tactggcttt cttattttcc cgatcgtttc cggtttcaat tcaacgggca
      781 caacatcgtt attcttcacg aaattgaaaa cggcttccgc gtgagcgatc ccgttttgga
      841 taaaccggtg gattgccgga ccgaagacct gatccgggcg cggtttgccc gcggaccgct
      901 ggaaccgcac gggttcatgt attacccggt ctcggttaat cttcatcccg acttacgcgg
      961 cgcctgcatc aagggcatgc tggatacctg caacatgatg ctcagaattc cggtaccgat
     1021 tttcggcatc cgcggcatcc gcttcctggc gaaacgaatc atcaaattcg agaagacgca
     1081 ggggttcgaa gaagcctgcc ggcagttggg catgatcgtc cgcatgcagg aggaagtcgg
     1141 cacgggcggc gctggattcc gctatatgta cgcagccttt ctgcaggaag ccgcggacct
     1201 tttcggctcg gcggaactca acgatctatc gcaggacatg acggccatcg gcgatacgtg
     1261 gcgcgagttt gccgtcaccg ccgcgcgcat catcaaaaaa agaggaaaac aggatgagac
     1321 gtttgccaaa gcgggcggat taatgctgat ctgcgccggc cgtgaagaaa atctttttaa
     1381 aaatctgcat gagattgccg gaaaactgaa atcatgaaag ctctggacat tgcgcaagtc
     1441 actttttcct ataaccccaa agagggaaaa atcaaaaccg ccggggctga atatgcgctg
     1501 cgcgattttt ccatgtcggt ggacagcggc cagatgcacg cacttttggg acctaatgga
     1561 gcgggaaaga ccacgttgat gcgcctgatc accgggcagc tgcgtggatt tcagggcagc
     1621 atatcgattt acggcacccg caacgatcat gcacaatcgc tgagttccgt cggttacgcg
     1681 ccccagccca tctccgtata tgcgggactg accgccatgg aaaacatgcg cgttttcggc
     1741 gtcatggccg ggttgtcgcg cgagctgctg caagaacggt cccgggcggt tctggaagaa
     1801 accggccttt tgccttacgc gcagaaacgg gcgtcaacct attccggcgg gatgctgcgc
     1861 cgtctgaatc tggccgtcgc ccttctccac gcaccgcgcc tgctgctcct ggatgaaccg
     1921 accgttggcg tggacccgca gagccgcaac catatttatg aaactctgaa aaaaatgaat
     1981 gcctccggaa ttgcgatcgt tctgtgcacg catatcatgg acgaagcgca gcggatgtgt
     2041 tcaaaggtga ccatgctgga ccggggagcg gtgatttttt ccggcgccat gaacgaaatc
     2101 gaccatctgg aaactttttt tctggcacaa accggacaca gattgcgcga tgaggattaa
     2161 agaggggaaa cgaaagtgac gttccggaaa ctgacagcgt tgatccgcaa ggaaatgatc
     2221 attttgctgc gcgaacgcca gaccattccg cttttattca tcatgcccat ggcgctgatt
     2281 ttttttctat cgctggcgct gcagggcgtt tatatggata aattgacggg acggcagatt
     2341 ctgctgatta ttgaaaacga atcgcatcag ccgacggcga atttgcttga acaacggatc
     2401 agcgccaaca aaatgattca acgcgtccag agaccgccgg gattcgacaa cgacaaggtt
     2461 ttcgaaacca gccgggcgca tgcggtgatt ttcataccga agggatttga tgaaggattg
     2521 aaaccggtgg aaatggagtt cgatcccgtt ctcgacgccg gatacaaatc cgccgtgcgt
     2581 gttctggtca cgggcctgac cgtggaggtg gtcatgggca tcgacgacat cgaagggatg
     2641 tcggccgatt ttatcgttga aaagacaaaa acgaaccgca ccttgcccgg cccgctgcaa
     2701 aacagcgttc ccggctggtc gatttttgcg atgtttttca tagcgattcc catgtcgatc
     2761 ggttttctgc gggaaaaaaa cgacggcacg ctgcagcgga tttttaccta tccggtcagc
     2821 cccgctattg tcattctcgg caagctgatt ccttattatt tgattaatct gatgcaattt
     2881 gctttaatgc tgatcgtcgg tatttatatt ttgccgctga tcattgcatt cccttttacg
     2941 ctgggagagc atccctggca tgtgataccg gtcacgctgg tcgtcgcggc cgcatctacc
     3001 gggttcggcg tcatggtcgc ggcgctggca cgggcgccgg aacaatcttc ggcgctggcg
     3061 gccaccggag cggtactgat ggccgtcttc ggcggtatta tggtcccgca cgcggtcatg
     3121 ccgctcatca tgaaaaaact ggcgatgctc tcaccgatgt actgggcgca tcaggcttat
     3181 ttggatattt tttcgcgcga cgccgggttc gtttccatat ggccgaagct tgctgtttta
     3241 acggtattcg cgttggtttg tttttatata gcggggagga aagttaaatg gatgtaaccc
     3301 agaaattgaa agacttaaac gtgtcatcaa gagaagagtt gatttacaag ctcaaggagc
     3361 tggtcatccg ggcttgtgaa atcacggatg tgcggtcgga agacgtgccc accgatgttc
     3421 ccttcatcgg cggtcccgga ccgctgctgc tggacagtct cgacgccatg gaaatcgcca
     3481 tggaaatcag atttcagttc ggtgtcgaat taaaaaacgc ttcaacggcc gccaaagcaa
     3541 tgcagaattt cgacacgctg gccgatttta tcatcgaggc tccaagggtt aaaaaatgaa
     3601 cagcggcgat atttatgtcc gcggctgggg cgcggtaacc accctgggat ggaatgccac
     3661 cgatgcggca cgcaacctga ttgaggctca ggccccaccg gcaaaaccgg ggcggtcttc
     3721 ccatctgatt aaccgcgact tcaatgtttt cgaagtgtcc catccgggca accctctgga
     3781 aaaagccatg gccatgctgg aaggcgcgat cgatgaggcg tttgagcgcg ccggcctatc
     3841 cccggatgaa atcgccgaat ccagcgtcct gatcggcacg accggcggcc tttttatccg
     3901 caacgaacat gaatttatcg aagccgtccg caaaaatccg gacggcgaaa cgccgccgat
     3961 ttcctgccgc aaccgcggtg ccggggaagt ggccgatttc atcaccaaaa aatacggtat
     4021 ccggggaatg gcgctgacgt tcagcatcgc ctgcgtttcc agttcgcatg cgctggtagt
     4081 agccgccgat cttcttcaga gcaggaaaat ccgccgggcc atcgtcatcg gttttgaacc
     4141 gcttctgaat atgacgctcc acggtttttc cagcctgctg ctctttgatt acgagcgatg
     4201 ccggcctttc tgcgccaccc gcgccggcat gcagatcggc gaagccgcgt ccgcggtcat
     4261 tctggattcc ggtcaaaatt tgtcctcccg ctatacattc agcggcgggt atctctacaa
     4321 cgatatcaaa agtatgacat cggcgggcat cgacggcgtg accgtaagcg aggtcgtgcg
     4381 ccataccctg gactcggcca ggctatcggc cgacgatatc atcgccatca aagcgcacgg
     4441 cacaggtaca aaagacaatg atctttccga aggccgggga ctggtcagac tcttcgggga
     4501 aaaacttcct ccggtggttt cccttaaggg cgccatcggc cacacgctgg gcgcggccgg
     4561 cacggcggaa acggctctct ggctttcctg cctggacgag ggaatcatcc cgcggagttt
     4621 cggtttcagt aaaatggatc cggaaatcgg ctttacccct tcgcaaaaaa atattgacgc
     4681 ccgcgacggc gcttacctgt ttacgttttt cggcttcggc ggaacatgca caagttattt
     4741 gattatcagg aactccaccg aaaactcctc cgctcgtgcc ggctcatcat aacaggtcct
     4801 gaaagataca accctatcgg ataagggaat gcatatgaca gaaacgacga ccaaacacac
     4861 ggccccggcc tcacccatac cggtgatcgc ggcaagttat atcggtcccc cgctgatgcc
     4921 caaccgcgat gcgctctggg aagagaacga attgcccttg aatgccgatg ctctggtcaa
     4981 cgaggttatg ggacagccga tgcgccgcgc gggaagattc atcaagatgg tttcctgcgg
     5041 cggcctggcc tgcctccagc aggtctcacc cgattatctg aaggggaaga aaacgggaat
     5101 ctttctggcc accggtctgg gcaatgttga cgaaatcctg cctttcatca cacaggtgtt
     5161 caaacatggc ggcgggtttc ccagtcccaa ccagtttgcc aattcggtca gcaatgccgc
     5221 cgctttcgtg atggcgcgga tttgcgaggt taccggaacc gttctgaccg tctcacagga
     5281 agaattatca ttcgagtcgg cgctctggct ggcacaaacc tatctggaac acggccggat
     5341 agacatcgcg ctggtcggcg gctgcgatat ctttacaccc acggctgaag aataccgcga
     5401 acgggtgaat gtcagctgcg gtaatgcccg cagactgccg atgggcgaaa gtagttcctg
     5461 gctcctgctg ggcaagaatg aagaacaaaa tatagggcat attctagccg tggattttta
     5521 cgacactgtc ggcgcgccgg accaacgttt cgataagcaa acgattctgt cagatctttg
     5581 tgatgcaaga ggaaaaggcc ggattgttct tcccggattc cgcatcaggc aaacggacct
     5641 gaacggctgg aaagacggcg gctgggaaac ggaagattat ctgccctgct gcggaataca
     5701 ttccacggca tccgctttcg gcatcgcgcg aacgctgcgc gaaaaaacca attccgtttt
     5761 tgtgcattat aactgcagtg cctcaggcag gcatgctttt gtttcaggct caacaaaatc
     5821 atgatggttt cttcccggca gaaaaaacgc attgttcttg tccatccccg gggattcaac
     5881 tggtttcccg gcaaacgcga tatcaccgat atcgccaatc ggatggtgcc gcagggcgtg
     5941 ctgtccatcg ccgcctatct gaaacaaaaa ggacatgagg ttttcgtcta cgactgtctg
     6001 ggaccgggag cgccgctcga tctgcaaaaa caggccggcg ccattctggc ctatcagccg
     6061 cagatggttg gcttttcggc cacaacatcc tcattccccg atgccgctga tctggcccgg
     6121 ctcatcaaaa atcattcttc agccatcgcc accgtctgcg gcggcgttca cgtatccgct
     6181 ttggaaggaa aacttctgaa cgcttacccg gcatttgatt ttctcattgc cggagaaggc
     6241 gaggagacca ttgccgaact cgcggacgag attcccgccg ccgacatccg gggcttaatc
     6301 tggcgcaacc aggacgaaat cacggtcaac gatgtgcgcc cgaagatatc cgatctggac
     6361 agtctgccct ttcccgctta tgaacagctc aaaggtttcc cccgcgatta ccacctgccc
     6421 ctgttcagct acatccatac gccgggcgcg accatgatta cgtcgcgggg ttgtctgtat
     6481 cagtgctcct actgcgaccg ctccgttttt aaaaaaggct tccgctacaa ttccgccggc
     6541 tacatttacg accacatgaa atatctgcgg gagacattcg gtgtacggca catcaacatt
     6601 tacgacgatt tattcaccgc caaccgtccg cggattgtca agctctgcga aaaactcagc
     6661 cgccgtcctc tgggtctgca tttcaactgc gccgtacgca tcggttacac ggatgatgat
     6721 ttgcttgaca tgctcaaaga cgccggatgc ctgatggtgt cgctgggaat cgaatcggcc
     6781 gaccccgcca tgcttgcccg gcacaaatcg ggcatcaacc tggatgacgt gcgcgacaca
     6841 gtaaaacgaa ttcaaaaagc cggcctgcgc gccaagggtc tttttatgat ggggctgccc
     6901 ggcgaaacgg aggcatccgt caaacgcaca tcggatttta ttctgtcgct ggacctcgac
     6961 gatatgaaca tggccaagtt tacgcccttt cccggcgccc cgctgtggac ggccatcaga
     7021 gaggagggga cttttcacga agactggcgg ctgatgaact gtctgaattt tgtttttgta
     7081 cccaggggca ttgcgtcgaa ggaacggctg gatcagctct ataacgaata cgtgaagcgt
     7141 ttttattctg ataaaaaatg gcgtaaaaaa ttccgcaatc gcatctggca gcatcgcaat
     7201 agccttttgt acttaatacg tcatctgccg tctttctggt cggcgaaaaa tcagttcgaa
     7261 ccgcaaaaag gaaaataacg taaaaaacca catcgggcgg cacaacggag tttgtgcaca
     7321 gactgcatgc gatgagccgc tttgcatcaa cggcgccgct ggttgtcgtc gaaaaaacct
     7381 tcttcttttc acggcggctt tttatctggt aaacgattcc gacggccccg gccggtccgt
     7441 attgactgat tttggaaaaa agtttgcaac ggccgcaagg caggtgcagt tgcctctgct
     7501 cggagttttc agcagacgga agcaggcatt attctttcac cccggatttc ttaagccttt
     7561 ccacaatgtc ggtccattta ttgatctccg cagccgtcag taacgtatat tccgtatctt
     7621 tgatcttcgc tctgacgttg ccatccgctc ctttatccag cagaagagtc accacgtcct
     7681 tatggccttt ctgtgcggca agaagcacgg ggggcaaacc atcgctgtct tttgcattta
     7741 catccgcccc cttgtccaga agaatctgaa taatgtcctt gtgtcctctt tccgacgcca
     7801 tcagcagagc cgttatgttt tcattacttt tgccattcac gggaactcct ttatccagca
     7861 gcagttgcac aacggcccgg tggccgttca gggacgccac catcagcaca gtaatgccgt
     7921 ctttgttctt cgcattcata tccgcccctt tatcgatcag cagacgggcg acatccgcat
     7981 ggccttttgc cgccgtcaaa atcagggcgg tcgcaccgat gaattttgtt ttcaaattga
     8041 catcggcccc tttattcaga aggagtccga cgatnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnnnn nnnntgaatt ttgttttcaa attgacatcg gcccctttat tcagaaggag
     8221 tccgacgatt tccctgtgat tgttttcggc ggccatcatc agcgccgtcg cattgtcgga
     8281 agctctctga acattcacgc cggcatctgt ttccaaaaga cgctcaacaa ccttcgcgtg
     8341 accgtttttc gacgccagca tcaacgccgt cacgccgtcg ttgtttttga tcttgacgtc
     8401 cgcttggtgc tgcacaagac gctggacgat gtcgtgatga ccgtactgcg ctgccagcat
     8461 cagcgccgtt acgccgtcat tgtttcgggt gttgacatcg actttcccct gcatcagaat
     8521 accgaccgca tccggatggc cgtttttcga ggccagcatc agtgccgtta tcccggtgtt
     8581 gtttattgca ttaatatccg ctttgccttc cagcagtatt ttcatgacat ccgcatgacc
     8641 gttttccgcg gcaagcatca gggcggtggt tctttccgga agcctctgga agttgggatc
     8701 ggcgcctttc tgcagaagca gtttgacgat ttccgcatgg ccgttttccg acgcggcaat
     8761 caacggcgtt atctccttgc cgaaaatgtt ataatacttt ttaaccgatt tggcgttcgt
     8821 atcggcaccc tgggcaagcg ccgcctgaac gtcctgcaaa agaccttttt ccgctgcctg
     8881 aatcaaccgg tcattatttt gggccgcacc ggcatccgcg caaaccaatg caacaataaa
     8941 aagccccacg ggcacacgca gaatatgctt taccggacaa actgcgttct ttatcttttt
     9001 ccaaaacatt tttctttcca tgcgtaaacg atataaggaa gttttttgcc tgtcaataaa
     9061 atcatggaca tcatccaatt ttgtgcagag ccttcgctgg tttgaaattt tgtttattac
     9121 agaaccaggc cttttaattc aaccttgttt taccttccaa tatctgataa ttcagcttca
     9181 ggattagcca tatggaacaa attctgaccg tttcagaatt aaataatacc attaaatctc
     9241 ttctggaaga atcgtttgac tcccttctgg ttgaaggtga agtagccgat ttgcggcgac
     9301 cgcaatccgg tcatacctac ttcacactca aagacgacaa aagtcagatc cgggcggttt
     9361 tttttcgtcc gttcggaaga accgggggcc gtgtcaattt tgaactggag gaagggctga
     9421 agattttatg ccgggcgcgc ctgactcttt atccgccgcg cggcgaatac cagcttgttg
     9481 tggaatcggt ggagccactc ggtgtcggcg ccctgcaaaa ggctttcgaa cagctcaaag
     9541 ccaaactttc cgcagagggc ttatttgaca aacaatataa aaaagctctc ccttttctgc
     9601 cggaaagaat cggcgtcatc acctctccca ccggcgcggt gatcagagat attctgaatg
     9661 tcagccggcg gcggtttccc gcaacggaca ttttaattgc cccggtgcgc gtccagggca
     9721 atgacgcggc aaacgacatg attcaggcgt tgcgccgttt ccacgccttc gggcaggtgg
     9781 acgtcattat ccttgcgcgg ggcggcggat cgctggaaga tctggcttct ttcaatgatg
     9841 aaaggctggc tcgcgaaatt ttccgttcgg acattcccgt tgtatcggcc gtggggcatg
     9901 aaaccgattt taccatttgc gatttcgctg ccgatttgcg cgcacccaca ccctcggctg
     9961 ccgcggagct cgtcctgccg gaccgaacgg aactgatatt taaggttaat atgctggccc
    10021 gtagaatgac tgccggctgc ttgcgtcatc tgaaggctga aaaagaacat ctggaggggt
    10081 tgcaggaaag atttaaagac ccgcgcctgg cggtcgtcac tcagaaaatc agaattgacg
    10141 atctcaaaga gagcctttcc gccgctttcg gtcatcgccg gcaaatcctg ctccataagc
    10201 tgaaccacct ggagctgcgt ctgaaacacc agagtccgga gcagcagatc aacgaaaaac
    10261 aaatcgtatt aaaaaatctc cggaaagaac ttcacagcag cctgtcctgt gttctgacgg
    10321 cattgaagga acgatttcaa aaggatgctg cggtcctgga atcgttgagc cctcttgcgg
    10381 ttctggaaag aggctacagc ctcacgcgca cggtccccga aggcacgctc atccggcaga
    10441 cccgggacct gcatgaaggt gatgaggtca atgtcctgtt ggcaaaaggc aattttaacg
    10501 ctaaaataca aaaaatattt tagtgagtcg agatgtgttt tctgttagac agcatatcct
    10561 tgaatccgtc aaaaagtttg aacgggtttg aggcagtgga acgaattcat gaaagaacgt
    10621 ctcccgttta ccggggtcag gagcaatgat ggcaaaagaa aaatttgaag acgcactgga
    10681 aaaactggaa gatattgtca agaaaatgga ggcgggcgat atgcctctgg atgagacctt
    10741 gaagtccttt gaagaaggca tcaagctgat tcgcttctgt tccgccaagc ttgaagaaac
    10801 ggaacgccgc gttgaactgc ttctggagaa ggaaaacacc ttgcaggtaa aacgttttca
    10861 gggagacgac catgatgcag gatgatgatc tgttcctcaa ggcgtatctg gaggacagac
    10921 agaacattgt tgaagaagct ctgcagcgtt atcttcccga ggacggattt ccttccgaca
    10981 tttacaaggc tgtgcattac agtgttttca acggcgggaa acgcatccgt ccgattctct
    11041 gtcttgcggc cgcggaagcc gtcggcggcg atctggtccc ggccatcccc gttgcgtgcg
    11101 ccctggaatt aatccactcc tactcgctga ttcacgacga tttgccgtcg atggataacg
    11161 acgatttccg gcgcgggaaa cccacctgcc ataaagtttt cggggaaaac atggcgattc
    11221 tggccgggga tgcactgctc acggaggcgt tcgtgctttt gtcccgtgcg gaaaaggtca
    11281 tgctctccgc ggaacggcga ctggccatta ttcaggaaat tgcaaatgcc gccgggatca
    11341 acggcatggt gggcggtcag gcgcttgacg tgatatcgga aaaaaccgga gcggatgaaa
    11401 caattctgca ggacattcac cggcgcaaaa ccggcgcatt gatcgtcgcc gccgtaaaat
    11461 ccggcgcggt gatgttcaat gcaggcaaag ataaaattca agcgctgacc gaatacggca
    11521 tgcatgtggg actggctttt cagatagcag atgacattct gaatgttgaa ggagacagcc
    11581 ggttgatggg caagggaacc ggcagcgatg ccgcccgcaa caagatgacc tatccttcgc
    11641 tgatgggact gcaacaggca aaagaaaagc tgaaccagca cgtggaagcg gccgtcacca
    11701 gtctggccga ttttgatgaa agagccatgc cgctgattgt catcgcccgt tacatcaggg
    11761 acaggaagtc ataaaaaacg aaaggatttt ttttggtcaa gcaaccgcaa accgttcatt
    11821 acagtgttct gaaggatgtc aatttcccgg ccgatattcg cggactgacc cttgcccagc
    11881 tcaatacgct ggctcaggaa atcagggatt tgattaccct cacggttgca tcgtgcggcg
    11941 gccatctggc cgcgtcgctg ggaaccgtcg aactgaccct cgctcttcac tacgctttca
    12001 acacagcgca ggataaaatt atctgggatg tcggccatca gtcgtacgcg cataaaatca
    12061 tcaccggacg gaaagaggat tttaaaaacc tgcgcaaaca aaacggcatc agcgggtttc
    12121 cccggcggga ggaaagcccc tacgatgtgt ttaatgtcgg ccacagcgga acctccattt
    12181 ccgcggccgc gggcatgacc gaagccgcct gcctgaaggg cgccagctat aaaatcattg
    12241 ctgtgatcgg cgacggatca atgacctcag gcatggcctt tgagggcctg aactgggtgg
    12301 gagaccgcaa gaaaaacatg atcattgtgc tcaacgacaa tgaaatgtcc atctcggaaa
    12361 acgtcggcgc catgtcctcc tatctgaacc gggtgatgac cggtcataag atcaccaccc
    12421 tgaaagcgga tatcaaaaga tttctcaaaa gcattcccgg cgtgggcgga gatatgatca
    12481 aattcgccca gaaaatcgaa gaaattctga aaaccctgat ggtgcccgga gcgctctttg
    12541 aagaaatggg ttttacctac gtgggaccgc tggagggaca tcgcctcgat catctgatta
    12601 aaacgttcga aaacatccgg gagatgaccg gtcccgtgct tgtccacgtc attaccaaaa
    12661 aaggcaaggg ctacagtttt gccgagcaga atccgccgag ttatcacggc gtcgcccctt
    12721 ttgatgtgca aacaggccag gtcattgctt cctcgtccgg catccccact tacacaaacg
    12781 tattcggcag gacgctggtg aagctcgcgc gcaccgattc ccggattgtg gcgattaccg
    12841 ccgccatgcg cgaaggcacg ggcctcaatc tcttcagtca ggaattcccg aatcgttttt
    12901 atgacgtggg cattgcggaa cagcatgcgg tcacctttgc cgccggcctc gccacggaag
    12961 ggttcattcc cgttgtggcc atctactcaa ctttcctgca gcgcgcctat gatcaaatcg
    13021 tccacgatgt gtgcctgcaa aaacttccca ttatctttgc cgtggatcgc ggcggactgg
    13081 tcggtgagga cggcgccacg catcaggggc ttctggatta ctcctatctg cgttccattc
    13141 cccacatcgt ggtcatggcc cccaaagatg aaaacgaact ccagcatatg ctcaaaaccg
    13201 ccgtcgattg cggacagccg gtttctctgc gttacccgcg cgggagcgga gcgggcagac
    13261 ccctggatga ggttctgacg gctctcgaca tcggaaaggg agaagtgctc aggggaggaa
    13321 ccgatctggc cgtcatggca atcggttcaa cggtgctgcc tgctctggcg gcggcgaacc
    13381 gcctcgccga agagggacgg gatatcagcg tcatcaacgc ccgctttgtg aaacccctgg
    13441 atgaagaact gattctgaaa acggcggccg ctgtcgggac catcatcacg gttgaggaaa
    13501 acacactgca gggcgggttt ggaagcgctg ttctggaact gctggctgaa aaaagcatca
    13561 ccggcatccg ggtaaaacgt ctgggcattc cggacgaatt cgtcagacat gcaacgcagg
    13621 cgcagcaacg ccgacggtat ggaatcgatg aagaagggat tatggccgcc atcaacgaaa
    13681 ttctgacgtc tgaatcattc cgacgaccgt cttgagcctg ctcgacttct tgacttgaag
    13741 gacatcaaca atcctctatg gctgccgaga aaattcgttt ggacaaacta cttgttgaac
    13801 gcggaatcgc gcctaccctg gaaaa
//
LOCUS       DAOOLB010000055        13544 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_4979_length_13544_cov_51.056713, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000055 DAOOLB010000000
VERSION     DAOOLB010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13544
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4979_length_13544_cov_51.056713"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..510
                     /gene="rplM"
                     /locus_tag="PLF77_07595"
     CDS             79..510
                     /gene="rplM"
                     /locus_tag="PLF77_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPC08755.1"
                     /translation="MMKTYMAKAQTADRKWYIADASGKVLGRFASEIAHRLRGKHKAT
                     YTPHVDTGDFIVVVNAEKIVLTGKKMTDKIYYSYSGYPGGLRETTAGKMLAEKPEDLI
                     RIAVQGMLPKTNLGRRMLKKLKVYSGNAHIHEAQCPKTLSI"
     gene            552..944
                     /gene="rpsI"
                     /locus_tag="PLF77_07600"
     CDS             552..944
                     /gene="rpsI"
                     /locus_tag="PLF77_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPC08756.1"
                     /translation="MAEQRFYATGKRKSAIARVYMKAGNGNIVVNKRSYEEYFPRPSL
                     KMLIKQPLDITGKKDQFDLYINVTGGGVAGQAGAVKHGISKALLEYDAELRSVLKKAG
                     FLTRDARVKERKKYGQPGARKRFQFSKR"
     gene            992..2029
                     /gene="argC"
                     /locus_tag="PLF77_07605"
     CDS             992..2029
                     /gene="argC"
                     /locus_tag="PLF77_07605"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HPC08757.1"
                     /translation="MIKAAIFGASGYTGQELIRILSGHPDVKLVAATSRRFAGQEVST
                     VFPALQGLTALKYQNATPCEICRLCDVVFLALPHGVSMDMAPEFINAGKTVIDLSADY
                     RLRDLKIYEVWYGKHASAKFIKNAVYGIPELYRQKIKKTKFVANPGCYPTGIILGLAP
                     ALKKQLLDVSTIIADSASGVSGAGREPLAGSLFCEVDGGFKAYKVGKHRHLPEIEQEL
                     NALAGKKFAVSFTPHLLPVKRGILSTIYAKLKKDIALGDLHALYSSFYAGETFVRMCP
                     EGVYPNISSVCGSNYCDIGLAVDPRTKRVIIMAAIDNLVKGAAGQAVQNMNIMCGFKE
                     DTGLQIAPLFP"
     gene            2048..3529
                     /gene="cysS"
                     /locus_tag="PLF77_07610"
     CDS             2048..3529
                     /gene="cysS"
                     /locus_tag="PLF77_07610"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPC08758.1"
                     /translation="MPQKIFNTATRKKEVFQPIQEGAVGIYVCGITAYDVCHIGHARA
                     AVVFDVVVRYLRAQGYNVTYVKNFTDIDDKIIDRANREGVSIADIAERYIKAHDEDMA
                     ALGVRTPTITPRATENMQEMIALIRTLEDKGYAYCVEGDVYFSISDYKQYGGLSGRNL
                     EEMMAGARVDVSDKKKNPLDFALWKNSKEGEPFWESPWGKGRPGWHIECSAMSRRYLG
                     ETFDIHGGGEDLIFPHHENEIAQSAAATGKPLARYWMHNGFVKINSEKMSKSLGNIFP
                     VKEILKHYHPEVLRLFMLQSHYRSPVDYSDDSLSEARAALFRCYRTLQQMKELQGQLA
                     AGSKPVATQPSGRVDDYVNAFQMLKDKFNEAMDDDFNTAQALGYVFDMVRQMNNFMMK
                     EKSMPVADKTAVLAAAIRVFGYFGSVLGVINSDADQFFVLYRETELRRRELNADEIEE
                     LIRQRQSAREAKDWARADDIRKKLAGMNVVLKDSADGTSWTIE"
     gene            3651..4973
                     /locus_tag="PLF77_07615"
     CDS             3651..4973
                     /locus_tag="PLF77_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPC08759.1"
                     /translation="MMKKLSIKFYLLAVIGVGALILLGLSSDNSAAAKDKNFYKDIKT
                     FSEVLDMVRKNYVDDVDTTNLMQGAINGMIRSLDPHSSFMTPDLYKELEVETQGQFGG
                     VGIEIMLIKDILTVVSPIEDTPAFRAGLKPGDQILKIDGKSTKDITIMEAVKQLRGAK
                     DTKVTITIMREGMAAPKDITLTRAIIQVKSVKHKKLEDNVSYIRIAAFQDRTADDLRK
                     ALRELDDKNKPMKGLILDLRNDPGGLLTQAIDVSDIFLKSGTIVSTRGRVKAMETKSV
                     AKNDNNEISCPIVVLVNEGTASAAEIVAGALQDNGRALIVGTQTFGKASVQTVIPLED
                     GSALKLTTARYYTPNGRSIQAEGITPDIIVPFIKGAEEAESVTDKEERIREKDLKGHI
                     KPDKDDGTKVDGGQSKDTKDPLDLEKDNQLQTAVNILKSWNIMKSIKK"
     gene            5010..5876
                     /locus_tag="PLF77_07620"
     CDS             5010..5876
                     /locus_tag="PLF77_07620"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HPC08760.1"
                     /translation="MKKIFTATLVVLILLSVAGIIYVVRFQEAVPEKAKEKEVAVKPA
                     APKIDSVKKRTSPPARLREVAIIIDDIGYDLNVAKALLKIDADLTFAILPFQRHSREA
                     AEMIYQANREVLVHLPMEPVSYPRERPGEGALFTDMSQEELVLQLRKNIDAVPHAVGV
                     NNHMGSKFMMDDEKLSVIFKELQRKKLFFIDSRTSPDTRAYAIADDMGFRIAERKIFI
                     DHHRDYHAIYSQLMHVAQSNDPAPKIIIGHPYPETVRALQAATKVMREKGVSVVPASR
                     IIKKTNKSPEKS"
     gene            5926..7614
                     /locus_tag="PLF77_07625"
     CDS             5926..7614
                     /locus_tag="PLF77_07625"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC08761.1"
                     /translation="MKRNFICFVVIMFVSLSGCVTLPNVQHAPSGDKFQQQASYHYLL
                     SVFLRNDGNLDEAIRELEQAYAADPDSAYLVIELMSLYAQKGDVKSAVAHGEEALARD
                     PDNVDVHLIMGGLYLNMREYSKAVREHKKVIELDPKNLVAHLYLGIIYAEEKRFDRAE
                     ETFKKMLKIDPDNIMGVYYYGKVLEKMKRLDEAEKMFKKAMSLNPYFEMALFDLAALY
                     EKQEKLDKAIEIYRNYLAIDPTRLNLRVRTGELLIKKKKYEEAEKEFQEVLKIDPDNR
                     DIKVALGLLYFDMKKFDLAAAEFSSILQKNVLDDKVRYLLATTYDQQKNNVRAVEEFT
                     GIASSSELYVSAQLRAAMILKEEGKVTEAVAVIKKSIKKKNDQVPFYLLLSSFYDETG
                     DNASSEAILQDGLKVLPRSTDLHYALGVLYDKTNRFEESIMSMKKILDIDPKNAEALN
                     FIGYSYAVRDMHLDEAEAMILRALKIKPGNGYLLDSLGWVYFKKNDLKNAEKYLKEAW
                     TLLPDEIEIIEHLGDLYVRQNRLKDAADMYERGMKIDPGNDVLKKKREDIRGTR"
     gene            7690..8427
                     /locus_tag="PLF77_07630"
     CDS             7690..8427
                     /locus_tag="PLF77_07630"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HPC08762.1"
                     /translation="MLCLCSCALQQASGVARDVYPEKRILSTSGGLAEEDVFQALAKI
                     DFKTVDASYHVRAALVLRRPGYVRLEVLPLIGNPDLLLTATPDTMTVFVPSKKELYRG
                     RPTRSNLEKFLPWSMDIEDMVLVLTGTYPFCTTERLAEDVSSEGRLWRMTCKKSSSES
                     QIIWVQEGSQKLMRLLCTDETGSEKYNVQYFYGGQSALPEKLVIRLANGSTALTVTYS
                     DVRVEKTKDLSVFNLTAPSDVEQNILE"
     gene            complement(8452..9264)
                     /gene="ybgF"
                     /locus_tag="PLF77_07635"
     CDS             complement(8452..9264)
                     /gene="ybgF"
                     /locus_tag="PLF77_07635"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HPC08763.1"
                     /translation="MERFRYFILFLVVLFLAGCASSGDLRALRSEMSQRTDEKISSLE
                     AEVNALKENATALESMRKGQANTMADLAELRENLQQLRGQVETLKKDASQSSRKDEQL
                     LNNMLLRINFIENFLEIGSKNTSDASDKTGKVNGGSVAKDTGSKQDKDKAYSAAYQIF
                     QEGNYAKARNEFQTFLTTYPNSEYSDNAQFWIGECYFFEKKYESAILEYEKVTKNYPK
                     GNKVPYALLKQGLSFLKLGDKTSAKLLLQEVIKNYPNTSQARIARSNLQDIK"
     gene            complement(9286..9855)
                     /gene="pal"
                     /locus_tag="PLF77_07640"
     CDS             complement(9286..9855)
                     /gene="pal"
                     /locus_tag="PLF77_07640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HPC08764.1"
                     /translation="MNMQIHLRLVLVVFIAGLMMIAGCAEKKSVITSDAAQEQQTTPA
                     QTTPSQEDMSSKDITGSQIASEEKSVDLEKMARAQSPVNDIHFDFDSSSIRPDARETL
                     RENADYFMNNRISSIIIEGHCDEKGTNEYNMALGERRAQETKKYLVNLGVKESLMQTV
                     SFGEENPLDPASNEDAWAKNRRAHFAVNP"
     gene            complement(10055..11377)
                     /gene="tolB"
                     /locus_tag="PLF77_07645"
     CDS             complement(10055..11377)
                     /gene="tolB"
                     /locus_tag="PLF77_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HPC08765.1"
                     /translation="MTKRVLILTLIFFFCAWPICSNRADSKVYVDIDSPAFRLIPIAI
                     CDFHPASGPVGHVASETTVSERVKKDLALTGLFNILNKQSFLESPDAVSTTAVGKIRF
                     ADWLAVGADYLVKGRIFQKDREMTVEAYLYDVARGELIFGKKYQAPAEKTKAVARAIA
                     SDIMLALINDAGDFNTEIAFVSKTGLRSDIYAVSYDGADVRKVTHHQSILMAPRWSPD
                     GNGMAFTSFKRGRPEIYFLNLKNRSEKRLASFGGLNLCGSFSPDGRKLLMTLSKDGNE
                     EIYALDVQTLQLQRLTRHSAIDVSPSWSPDGKQIVFVSNRGGSPQIYTMDADGNNVKR
                     ITYKGSYNSSPSWSPRGDRIIYEGLTADRYQIFSVDTEGNNPAQLTSDNANNESPFWS
                     PSGRQIVYVSRKHSGSQILIMNANGTNPRLLYEQSNRLAMPAWSGRLR"
     gene            complement(11429..12148)
                     /locus_tag="PLF77_07650"
     CDS             complement(11429..12148)
                     /locus_tag="PLF77_07650"
                     /inference="COORDINATES: protein motif:HMM:NF024503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPC08766.1"
                     /translation="MTPRMLGKGYRSGNGNFQNMIFLSLTLHVVVVALILVSVPTTSR
                     HLTFGAAYSVQLVGSEAVMPSQETSSLSDILESKKTSDAIIIKRKISSIYSSPLKTPA
                     TQKLDVEKAVGALRQKERAPGKTKENAPASGRTAMTDAEVNAQANEYIETVWSRVKQN
                     WSIPQSLLPDNNITTIVDVRIARNGALLYAGFEKRSGNRYFDDSALRAVKKSGPFPPL
                     PHWIRDNHIEIGIRFHSSELR"
     gene            complement(12145..12570)
                     /gene="tolR"
                     /locus_tag="PLF77_07655"
     CDS             complement(12145..12570)
                     /gene="tolR"
                     /locus_tag="PLF77_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HPC08767.1"
                     /translation="MVRGSRRKNNASKPLAEINVTPLVDVMLVLLIIFMVTAPMLSMG
                     IDVNLPRVKAKSVDVAEEKLVLTVNETREIFLNKTKMTLGELKPKLEAIFSNRIDREI
                     FLRADKNVPYGFVVEVMSEVRKAGVDKLGMITEPPEETK"
     gene            complement(12588..13313)
                     /gene="tolQ"
                     /locus_tag="PLF77_07660"
     CDS             complement(12588..13313)
                     /gene="tolQ"
                     /locus_tag="PLF77_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HPC08768.1"
                     /translation="MKDFGALDLSGSVNGSLLSMILDAGLMVKFVLLVLFIFSVVSWA
                     IIFIKYRYYRKIQKENEAFNDDYLKSSKLSDVLPAAKKYSYSTTAEVFRVGYKELTQT
                     NRPAAGESAQGRDDISLSSLDNLERSMNKASATEMTKLESSLGFLATTGSASPFIGLF
                     GTVWGIMDTFKGIGARGSATLAVVAPGISEALIATAAGLAAAIPAVIFYNYFLNQSKN
                     MVQEMENFSAEFLNIVERYLVRK"
BASE COUNT         3611 a         3053 c         3432 g         3448 t
ORIGIN      
        1 gttcaaatat ttttcttgac aagcaatttg aaattgcata aggtgcatct ttaaattttt
       61 cattaaagtg gtgattttat tatgaagacg tacatggcta aggcgcagac agccgacaga
      121 aaatggtaca ttgcagatgc ttccggtaaa gtgctgggaa gatttgccag tgaaatagcg
      181 caccgtttaa gaggcaagca taaggcaaca tacacaccgc atgttgatac cggtgatttt
      241 attgttgtcg tcaatgccga aaaaatcgtt ctgaccggca agaaaatgac ggataaaatt
      301 tattattctt attccggcta tcccgggggg ttgagagaaa cgaccgcagg gaaaatgctt
      361 gccgaaaaac cggaagattt gattcgtatc gccgtgcagg gaatgcttcc caaaacgaat
      421 ctcggacgca ggatgcttaa aaaattgaaa gtttattccg gtaatgctca tattcacgaa
      481 gcacagtgtc cgaaaacatt atcgatttaa cggacaatca actataaact agatttaata
      541 ttggagaaat catggcggaa caaaggtttt atgcaacagg aaagagaaaa agtgccatcg
      601 cccgcgttta tatgaaagca ggcaacggca atattgtggt taataaaaga agctacgagg
      661 aatatttccc ccgtcccagc ctgaaaatgc ttatcaagca gcctctcgat atcacgggga
      721 agaaggatca gtttgattta tatatcaacg tcaccggcgg aggcgttgcc gggcaggcgg
      781 gagccgtgaa gcacggcatt tccaaggcgc ttctggagta cgatgccgag ttgcgatcgg
      841 tcctgaaaaa ggccggtttt ttaaccagag atgcccgggt taaagaacgc aagaaatacg
      901 ggcagcccgg agcccggaag agattccaat tctccaaacg ttaaattccg acaggggggc
      961 ttccagcctc cctttttttt gggagcgttt tatgattaag gcagcaatat ttggcgccag
     1021 cggttatacg ggtcaggaac tgatcagaat tctgtcagga catcctgacg ttaaattagt
     1081 ggcagccacg tcaaggcggt ttgccggtca ggaagtatcg accgtgtttc cggctttaca
     1141 gggtttgacc gcgttaaaat atcagaacgc aacaccgtgt gaaatatgca ggctttgcga
     1201 tgtggtgttt ctggctctgc ctcacggtgt gtccatggac atggctccgg aatttatcaa
     1261 cgccggaaag acggtgattg atttgagcgc tgattaccgt ttaagagatt tgaaaattta
     1321 tgaagtctgg tacggcaagc acgccagcgc caaatttatt aaaaatgcgg tgtatggcat
     1381 tccggagctt tacaggcaga aaataaagaa aacgaaattt gtggctaatc ccggctgtta
     1441 tccgaccggc attattctgg ggctggctcc ggcgctgaaa aagcaattat tggatgtttc
     1501 cacgattatc gcggattccg cctccggcgt cagcggcgcg ggacgggaac cgcttgccgg
     1561 ttcgctgttt tgtgaagttg acggcggctt caaagcttat aaagtcggaa agcatcgcca
     1621 tctgccggaa attgagcagg aactcaacgc gctggccggc aaaaaatttg ccgtttcctt
     1681 tacgccgcat ttactgccgg taaaacgggg cattctcagc acgatttacg ccaaactgaa
     1741 aaaagatatt gctttgggcg atctgcatgc tctttacagc agcttttatg ccggggagac
     1801 gtttgtccgg atgtgtcccg aaggcgttta cccgaatatt tcgtccgtat gcggctcaaa
     1861 ttactgcgac attgggttgg ctgttgatcc gcggacaaaa cgggtcatta tcatggcggc
     1921 catagacaat ctggtgaaag gggcggccgg acaggccgtg cagaatatga acatcatgtg
     1981 tggatttaag gaagataccg gcctgcaaat cgcgccgctc tttccctgat gcgtaaaggt
     2041 gaaaaaaatg cctcagaaga tattcaatac agcgaccaga aaaaaggaag tttttcagcc
     2101 cattcaggag ggcgccgtcg gcatttatgt gtgcggcatt accgcctatg acgtctgtca
     2161 catcgggcat gcccgtgcgg cggttgtttt cgacgtggtc gtcagatatc tgcgggccca
     2221 gggctataac gtcacctatg tgaaaaactt cacggatatt gacgataaaa ttatcgaccg
     2281 ggccaacagg gaaggcgtga gtattgcgga tatagccgaa cgatacatca aagcacacga
     2341 tgaagacatg gcggcgctgg gtgtccggac acccaccatt actcccagag caaccgaaaa
     2401 catgcaggaa atgattgctc tgattagaac cctagaggac aaaggttatg cgtattgcgt
     2461 tgagggagac gtgtattttt ctatcagtga ttataaacag tacggcggtt tatccggtcg
     2521 caatctggaa gaaatgatgg ccggcgcccg tgttgatgtc agcgacaaga aaaaaaatcc
     2581 cctggatttt gcactctgga aaaacagcaa ggaaggcgaa cccttttggg agagcccgtg
     2641 gggaaaaggc cgccccggct ggcatattga atgttcggcc atgagccggc gttatctggg
     2701 agagacgttt gatattcacg gcggcggtga ggacttgatt tttccgcatc atgaaaatga
     2761 aatcgctcaa tcggcggcgg cgaccggcaa gccgctggcg cgttactgga tgcacaacgg
     2821 ttttgtaaaa atcaattcgg aaaagatgtc caaatcgctg ggcaatatat ttccggtcaa
     2881 ggaaatcctg aaacattatc atccggaagt gttgcgtttg ttcatgctgc aaagccatta
     2941 ccgcagtccg gtcgattatt cggatgattc gctgtccgaa gccagagccg ctctgttcag
     3001 atgttacaga acgctccagc agatgaaaga acttcaggga caattagccg ccggatccaa
     3061 accggtggca acgcagccgt ccgggcgcgt cgatgactat gtcaatgcct tccagatgct
     3121 gaaagataag tttaatgaag cgatggatga tgatttcaac acggcccagg cattgggcta
     3181 tgtttttgat atggtccggc agatgaataa ttttatgatg aaagaaaaaa gcatgccggt
     3241 ggccgacaaa accgccgtcc tggccgcagc catccgggtt tttggttatt tcggttcggt
     3301 gctgggcgtc atcaacagcg atgcagatca attctttgtg ttgtaccggg aaaccgagtt
     3361 gcgccgacgc gagttgaacg ccgatgagat tgaggagttg atcagacaaa gacaatccgc
     3421 ccgcgaagca aaggattggg cccgggccga tgacatccgg aaaaaactgg ccgggatgaa
     3481 tgttgttctg aaggattctg ctgacggcac cagctggacg atcgagtaat atcaaaacgt
     3541 ttccgtcttc gagagcactc aacgctttca gatattttcc aacaaataca tgataaatat
     3601 tgaagaaagt gatataagta atgcggtata tattcaattt tgtgaggaat atgatgaaga
     3661 agctatcgat aaagttttat ctgctggccg tcatcggtgt gggagccctg atcctcttgg
     3721 gactgagtag cgacaatagt gccgccgcga aggataaaaa tttttataag gacataaaaa
     3781 ctttcagcga agttctggac atggtcagaa aaaactatgt tgatgatgtt gatacgacta
     3841 atttaatgca gggagccatc aacggcatga tccggtcgct ggatccgcac agttccttta
     3901 tgacaccgga tttatacaag gaactggagg tggaaacaca gggacagttc ggcggtgtcg
     3961 gcatagagat tatgcttatc aaggacattc tgacggttgt ctcgcccatc gaagatacgc
     4021 ccgcgttcag ggccggcctc aaaccgggcg atcagattct gaaaattgac ggcaaatcga
     4081 caaaagacat tacgattatg gaagcggtca aacagctccg cggcgccaaa gacaccaagg
     4141 tgaccattac cattatgaga gagggtatgg ccgcgccgaa agacattacg ttaacacggg
     4201 ccatcattca ggtaaaaagc gtcaaacata aaaaacttga agacaacgtc agctatattc
     4261 gcattgcggc ttttcaggat cgaacggcgg atgatttacg caaggccctg agagaactgg
     4321 acgataaaaa caagcccatg aagggattga ttcttgattt gagaaacgat ccgggcggct
     4381 tgctgactca ggcgatagat gtatcggata tttttctgaa atcggggacc attgtttcca
     4441 cccggggaag agtgaaggca atggaaacaa aatcagtcgc taaaaacgat aataatgaaa
     4501 ttagttgtcc gatcgttgtt ttagtcaacg agggaacggc cagtgctgca gaaatcgttg
     4561 ccggcgcgct acaggataat ggaagagctt taattgtcgg aacacaaaca tttggcaaag
     4621 cgtccgttca gacggtgatt cccctggaag atggttcggc cctcaaactg acgacggccc
     4681 gttattacac acccaatggc cgttctattc aggcggaagg cattaccccc gacattatcg
     4741 tgccttttat caagggggcg gaagaggcgg aaagcgttac ggataaggaa gaacggatcc
     4801 gggaaaaaga tctcaagggt catattaaac cggataagga cgatggtaca aaggtcgatg
     4861 ggggccagag caaagacaca aaagacccat tggatttgga aaaagacaat cagctgcaga
     4921 cggccgtgaa tattcttaag agctggaata tcatgaaatc cataaagaaa tagcagccgt
     4981 tctgcactac ttgagcattc atgaatttaa tgaaaaaaat ttttacggca actctggtcg
     5041 tactcatcct attgtcagta gccggcatca tttatgtcgt gcggtttcag gaagcggttc
     5101 ctgaaaaagc aaaggagaaa gaggttgccg tcaaacccgc cgcgcccaaa atcgattcgg
     5161 ttaagaaacg gacatctcct ccggcacggt tacgtgaagt ggccatcatt atcgatgaca
     5221 tcggttatga cctgaacgtg gccaaagcgt tgctgaaaat cgatgccgat ctgacgtttg
     5281 ccattttacc ttttcaacga cattcccgcg aagcggcgga aatgatctat caggcgaaca
     5341 gagaggtcct tgtgcacttg cctatggaac cggtttctta tcccagggag cgaccgggag
     5401 aaggcgcctt gttcacggac atgagtcagg aagagcttgt gttgcaatta cggaagaaca
     5461 ttgatgcggt tccccatgcc gtcggcgtga ataatcatat gggttccaag ttcatgatgg
     5521 acgacgaaaa actgtccgtc atttttaagg aactccagcg caagaaactt ttttttatcg
     5581 attcgcgcac atcgccggat acccgggcct atgccattgc ggacgacatg ggtttccgta
     5641 tcgctgaacg aaaaattttt atcgatcatc accgtgatta tcacgctatt tacagtcaat
     5701 tgatgcatgt tgcccagagc aatgatcctg ctcctaaaat tatcatcggg catccgtatc
     5761 ctgaaacggt ccgggccctt caagcagcaa ccaaagtcat gcgggaaaag ggggtgtcgg
     5821 ttgttccggc atcccgaatc attaaaaaaa caaacaagtc cccggagaaa tcataatgcc
     5881 ggcgcatcat cgcgaaagcg ccgttaaaac ggattcgaag accttatgaa aagaaatttc
     5941 atttgtttcg ttgtcattat gtttgtcagt ctttccggct gcgtgacgtt gccgaacgtc
     6001 cagcatgctc cgtcgggaga taaatttcaa cagcaggcct cttatcatta tctgctgagt
     6061 gtttttctgc gcaatgatgg aaatctggat gaggccatcc gcgagctgga gcaggcctat
     6121 gcggcggatc ccgactccgc ctatcttgtc attgaactga tgtctcttta cgcccagaag
     6181 ggcgacgtca aaagcgccgt ggcccatggc gaagaggcgc tggcccggga tccggacaat
     6241 gtagacgtgc atttgattat gggcggcttg tacctgaata tgcgggagta ttcgaaagca
     6301 gtcagagagc ataaaaaagt catcgagctg gatccgaaga acctcgttgc gcatctgtat
     6361 ctggggatta tttatgcgga agaaaaaaga ttcgacagag cggaagagac atttaaaaaa
     6421 atgctgaaaa tcgatccgga taacatcatg ggcgtctatt attacgggaa agttctggag
     6481 aagatgaaac ggctggacga agcggagaag atgtttaaaa aagccatgtc cctgaatccg
     6541 tattttgaaa tggccctgtt cgatctggcg gctttgtacg agaaacagga aaaactggat
     6601 aaggccattg aaatttaccg caattatctg gcgattgatc cgacacggct taatttacgc
     6661 gtgcggaccg gcgagctttt aatcaagaag aaaaaatatg aagaagcgga aaaggaattt
     6721 caggaagttt tgaaaatcga tccggataac cgggatatca aagtggcgct gggtctgctg
     6781 tattttgaca tgaaaaaatt tgatctggcc gccgccgagt tttcttcgat tttacagaaa
     6841 aatgtcctgg acgacaaagt gcgttatttg ctggcgacaa cgtacgatca gcaaaaaaat
     6901 aacgtccggg ccgttgagga atttacgggc attgcatcat cgtccgagct gtatgtcagt
     6961 gcgcaactcc gcgcggcgat gatcttgaaa gaggagggga aagtcacgga ggccgtggcg
     7021 gtcataaaaa aatccattaa gaaaaagaac gatcaggtcc cgttttatct gttgttatcg
     7081 tccttttatg atgaaaccgg tgataacgcc tcgtcggaag ccattctgca ggacggtttg
     7141 aaagtgctgc ctcgaagcac ggatttgcat tatgccctgg gcgtgcttta cgataagacc
     7201 aaccgttttg aagagagcat tatgtcgatg aaaaaaatcc tggacattga tccgaaaaat
     7261 gccgaagcgc tgaatttcat cggatacagt tatgcggtaa gggacatgca tcttgatgaa
     7321 gcggaggcca tgatcttgcg ggcgctcaaa atcaaacccg gcaatggtta tttactggac
     7381 agcctgggat gggtatattt caaaaaaaat gatttgaaaa acgcggaaaa atatctgaaa
     7441 gaagcctgga cactgctgcc tgatgagatt gaaatcattg aacacctcgg ggatttgtat
     7501 gtccggcaga acaggctcaa ggacgcggcc gatatgtatg agcgggggat gaaaatcgat
     7561 cccggaaacg atgtcctgaa aaagaagcgc gaggatatcc gcgggacacg ataatcaaac
     7621 cacaaggtct ttccggataa gaatcaactc atgatccaaa aatatttccg cttgatatgg
     7681 atgtttctga tgctttgcct ctgttcctgt gcgttacagc aggcatcagg tgttgcgcgc
     7741 gatgtttatc cggaaaaaag gatcttgtcg acatccggcg ggcttgccga ggaggatgtt
     7801 ttccaagccc tggcgaaaat agattttaaa acggtcgacg catcgtatca tgtccgggcc
     7861 gccctggttc tgagacgtcc gggatacgtg cgcctggaag tgctgcccct catcggcaat
     7921 cccgacttgt tgctgacggc gacacccgat accatgactg ttttcgttcc gtcgaaaaaa
     7981 gagctttacc ggggacgacc gacgcgttcc aatctggaaa aatttctacc ctggtcgatg
     8041 gacatcgaag atatggtcct ggtgttgacg gggacctatc cgttttgcac gacagaacgt
     8101 cttgccgagg atgtctcatc cgaaggccgt ctttggcgga tgacatgcaa gaaatcctcg
     8161 agtgagtcgc aaatcatctg ggtacaggaa ggcagtcaaa aactgatgcg gctgctttgc
     8221 accgatgaaa ccggttcgga aaaatataat gtgcaatatt tttatggcgg tcaaagcgct
     8281 ttgcctgaaa aacttgtgat ccgattggcg aacggctcaa ccgcgctgac agtcacctac
     8341 tccgatgtcc gggttgaaaa gacgaaagat ttatcggttt ttaatctgac cgctccatcc
     8401 gatgtggaac aaaatatttt agaatagata cttgcagagg gtgaaaaacg actatttgat
     8461 gtcctgcaaa ttggagcgcg cgatacgcgc ctggctggtg ttcggataat tttttatgac
     8521 ttcctgcagg agtaatttgg cgctggtttt atcccccagt ttcagaaagg aaagaccctg
     8581 tttcaaaaga gcatagggga ccttgtttcc cttcggataa tttttggtga ctttttcata
     8641 ttccagaatg gccgattcat attttttttc aaaaaaataa cattcaccaa tccagaactg
     8701 tgcgttgtcc gaatattcgc tgttcggata ggtagtcaaa aaagtctgaa attcgtttcg
     8761 cgctttcgca tagtttcctt cctgaaaaat ctgataggcg gctgagtacg ctttatcctt
     8821 atcctgcttg cttccggtat cctttgcgac cgagccgcca ttgactttgc ccgttttatc
     8881 cgaggcgtcc gacgtgtttt tgctgccgat ttccagaaaa ttttcgatga aattgattcg
     8941 cagcaacatg ttattcagaa gctgttcgtc tttcctggac gactgcgacg cgtctttttt
     9001 gagcgtttcc acctgacccc gcagttgctg gagattttcc cttaattcgg caagatccgc
     9061 catggtattg gcctgccctt tgcgcatgga ttccagtgcc gtggcgtttt ccttcaacgc
     9121 gttcacctct gcttccagag aggatatttt ttcatccgtt cgctggctca tctccgaacg
     9181 cagcgccctt aaatctccgg aggaggcgca tccggccaga aaaaggacca ccagaaataa
     9241 aatgaaataa cgaaatcttt ccaaaggctt cacctcgctt tatcgttagg ggttgaccgc
     9301 aaaatgtgcc cgtctgtttt tggcccaggc gtcttcattg gaggccggat ccagaggatt
     9361 ttcttcgcca aaactgacgg tttgcatcag ggattctttc acccccagat tcaccagata
     9421 tttttttgtt tcctgagcgc gtctttctcc cagcgccata ttgtattcgt tggtgccttt
     9481 ttcatcacaa tgcccttcaa tgatgatgga cgaaattcta ttgttcatga aatagtcggc
     9541 attttcccgg agagtctcac gagcatccgg gcgaatactg gaactgtcga aatcaaaatg
     9601 gatatcgttg acaggggatt gtgcccgcgc cattttttcc aggtccaccg acttttcctc
     9661 cgatgcaatc tgggacccgg tgatatcctt cgatgacata tcctcctgcg agggggtcgt
     9721 ctgtgccggg gtggtttgct gttcctgtgc ggcatcgctg gtgatgacgg attttttttc
     9781 ggcgcaaccc gcgatcatca tcaaaccggc tataaatacg actaaaacaa gtcttaaatg
     9841 gatttgcatg ttcattgctt catctccttt tttaaaggtg ttcaaaaatt atattgatca
     9901 gaaaatgacg accaagaatc gttcacgtta aaaccatgca acaaaacgct gtttgtctgt
     9961 ttttgactcc cgtttcaccg aaatacattc taaatatgcg tgttttatac atcaaagaaa
    10021 agaaaataac aacccctttg agatttaaat ttgcctatcg gagtcgtccc gaccatgccg
    10081 gcatggcgag tctgttgctt tgctcataca gcagcctcgg attggtcccg ttggcattca
    10141 tgatgaggat ttgactgccg gaatgtttgc gggacacata cacaatctga cgccccgacg
    10201 gcgaccagaa cggcgactca ttgttggcat tgtcagaggt cagttgcgcc ggattgttgc
    10261 cttccgtgtc aacggaaaag atttgatatc ggtcagcggt taatccttcg tagatgatac
    10321 ggtcgccgcg cggtgaccag gagggcgagg aattataact gcctttgtaa gtaattcttt
    10381 tcacgttgtt cccatcggca tccatagtgt aaatttgcgg cgaccctcct ctattggaaa
    10441 caaaaacaat ctgcttaccg tcaggcgacc atgacggcga aacatcgatg gcggaatggc
    10501 gggtcaggcg ctgaagctgt aacgtctgaa catccagggc atatatttct tcgttgccgt
    10561 ccttgctcag ggtcattaat agttttcgac catcggggga aaaggaaccg cataaattca
    10621 atcctccaaa agatgccagt cttttttccg acctgttttt taaattgagg aaataaattt
    10681 caggacgtcc tcttttgaat gaggtaaagg ccattccgtt tccgtcaggc gaccagcgcg
    10741 gcgccattaa gatggactga tgatgggtga ctttcctgac atcggcgccg tcataactga
    10801 cggcataaat atcagaccgg aggcctgtct tggatacaaa ggctatttcg gtgttgaagt
    10861 ctcccgcgtc attgatcagg gcaagcatga tgtcggatgc tatcgcgcgg gcgaccgcct
    10921 tggttttttc ggccggcgcc tggtattttt tgccgaaaat caactcaccg cgggcgacat
    10981 cataaagata ggcctcgacg gtcatttccc gatccttttg aaagatgcgt cctttgacca
    11041 gatagtcggc gcctacggcc agccagtcgg caaaacgaat ttttcccacg gctgttgtcg
    11101 atacggcatc agggctttcc agaaaacttt gtttgttcaa aatattgaac agtcctgtca
    11161 gggcgagatc ttttttaacg cgttcggaaa ccgttgtctc ggaagcgaca tggccgaccg
    11221 gacccgacgc aggatgaaaa tcgcagatgg cgatgggaat cagtctgaaa gcgggagaat
    11281 cgatgtccac atacactttg gaatctgcac ggttgctgca gatcggccag gcgcagaaaa
    11341 aaaagatcaa cgttaaaatc aaaactcttt ttgtcacgtg atacccctac ctttttcttt
    11401 taaaaaacgt tctcctgcac acacgagatc agcgcagttc cgaggaatga aaccggatgc
    11461 cgatttcaat atggttatcc ctgatccaat ggggcaacgg aggaaacgga ccggattttt
    11521 tgaccgctct gagcgcggag tcatcgaaat aacggttgcc ggagcgtttt tcaaaaccgg
    11581 catacaacag ggcgccgttg cgggcaatcc tgacatccac aatcgtcgtg atattgttat
    11641 ccggcagcag ggattgcggt atggaccagt tctgctttac cctcgaccac acggtttcga
    11701 tatattcatt ggcctgcgca ttgacttcgg cgtccgtcat cgccgttcgg ccggatgccg
    11761 gggcattctc ctttgttttt cccggggcgc gttctttctg acggagcgca ccaacggctt
    11821 tttcgacatc gagtttttgt gtggccggcg ttttcagcgg ggacgaataa atgctgctga
    11881 tcttccgttt aatgatgatg gcgtcggagg tttttttcga ttccaggatg tcactgagtg
    11941 aagatgtttc ctgagaaggc ataaccgctt cagagcccac aagttgaacg gaataggctg
    12001 ctccgaaggt caggtgccgc gatgttgtcg ggaccgacac gagaatgagg gcaacaacaa
    12061 ccacatgaag ggtcagggaa aggaagatca tgttttgaaa gttgccgtta ccgctgcggt
    12121 agcccttgcc aagcatccgg ggtgtcattt tgtttcctcg ggaggttccg tgatcattcc
    12181 cagtttatca acccctgcct tacgaacttc cgacatgact tcaaccacaa agccataagg
    12241 cacatttttg tcggcgcgca gaaaaatttc ccggtcgatt ctgttggaaa agatggcttc
    12301 cagtttgggt tttaattccc ccagcgtcat tttggtttta ttcagaaaga tttcccgggt
    12361 ttcattgacg gtcagaacca gtttttcttc ggccacatcg acgcttttgg ctttaacccg
    12421 cggcagattg acatcgatgc ccatcgacag catgggcgcc gtcaccatga agataatcag
    12481 cagcaccagc atgacatcga ccagaggcgt aacgttgatt tccgccagcg gtttactggc
    12541 attatttttt ctgcgtgacc cgcgcaccat tgttttgtct ccgggattta tttacggaca
    12601 agatatcgtt ccacaatgtt taagaattca gcggaaaagt tttccatttc ctgaaccatg
    12661 tttttcgact gatttaaaaa ataattatag aagataaccg cgggtatcgc tgcagccagt
    12721 ccggctgcgg tggcgattaa tgcttcggaa attccgggag cgacaacagc cagcgtggcc
    12781 gacccgcgcg cgccgatgcc tttaaacgta tccataattc cccagacggt gccgaacaga
    12841 ccgataaaag ggctggccga cccggttgta gccagaaatc ccagcgaact ttccagcttg
    12901 gtcatttctg tggcactggc tttattcatc gagcgttcga gattatccag tgacgaaaga
    12961 ctgatgtcgt cgcgtccctg ggccgattca cctgcggccg gtctgttggt ctgggtcagt
    13021 tccttgtacc ccacgcgaaa aacttccgcc gtggtggagt atgaatattt tttcgctgcc
    13081 ggcaagacat ccgaaagttt cgaactcttc agatagtcgt cattaaacgc ttcattttct
    13141 ttttgaattt tgcgataata gcgatacttg atgaaaatga tcgcccagga aaccacagaa
    13201 aaaatgaaaa gcaccagcaa aacaaactta accatcagcc cggcatcgag aatcatgctc
    13261 aaaaggctgc cgttcacact gccgcttaag tctaaagctc caaagtcttt cacttttaat
    13321 ctccaatggg aaatgaattt gttgcgtacg ctaaaggaaa aaaaagtcct tgtcaatata
    13381 tcttttaacg taaaaaactt cttgtttttt tttagaaatg atagtagatg taccgataac
    13441 ttaaaaacta tttgattaaa acgtaataat aataaggggg acctatgaaa agaaaaagaa
    13501 ttttaactca atttttgtta tattttctga tgttgatgtt tttg
//
LOCUS       DAOOLB010000056        13462 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_5028_length_13462_cov_45.575744, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000056 DAOOLB010000000
VERSION     DAOOLB010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13462
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5028_length_13462_cov_45.575744"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2213)
                     /locus_tag="PLF77_07665"
     CDS             complement(<1..2213)
                     /locus_tag="PLF77_07665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC08769.1"
                     /translation="MKNNSCPRGAATPKHPQTPGKKDLKIIRYDEQLFRTLIEQSLDI
                     ILLINRQGIITYANTRAEKVMDFKKEDIIGNEIYTFIHGDDRAPTARVFNELLNNPDA
                     ELHRTEIRTIAKDKTVRTLEAVGTRVVQDGMQDVVLVNLRDITDRRKAEEALANSEAL
                     YRLLADNITEHIWIMDLNLKFTYISPSVEKIYGYPLEKIKTLKLTNLFTKESYQKLRD
                     TFATELPKAMAASPSPDKPRFVLELQAIHADGHLLWIENHLSFIRDENGKPVSIMGET
                     VDITDRKNVQDKLLEEEQRFRALADQSSDIIIMVNREGIVTYENPSVSILGFSVEERL
                     GNHAFEFLHPDDLETISRAFNLLFSDTNAPVQRSEIRLRHRDGSWRTFEAIGSNLIRN
                     NIIEAAIVNLRDITDRKQSEEALKKSEEKYRLLADHMKDQVWLMDLNMHITYVSPSVE
                     RITGYSADEIINMPWKRILTKESFDRVSDFIAAELPKALKSTSGNRLFKTIELEFILK
                     NGQTVWGECEFSLIRDEQGNPLSVLGESRNITERKLAEDKLQQTLESLKKAVGTTIQV
                     LISALEARDPYTAGHQSRTANLACAIAAEMGLDKDIIEGLNMAGIIHDIGKLSIPAEI
                     LSKPTGLTNIEFSMIKTHPESGYEMLKDVESPWPLSEIVYQHHERMDGSGYPRNLKGN
                     DILIEARIMAVADVVEAIASHRPYRPSLGLEFALKEIEKNRGKLYDEKVVDACLKL"
     gene            complement(2287..2694)
                     /locus_tag="PLF77_07670"
     CDS             complement(2287..2694)
                     /locus_tag="PLF77_07670"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPC08770.1"
                     /translation="MKEKRKSKRLHEFNEITVSVIPGDPNLPEKDLVYKYSEDISATG
                     AKIRGNILLPVDTLLKIDFTLKTLQEKITAQGKVKWIKVVFEDNWYEAGVEFVNTPKE
                     AIKKIEDYISWKQNSSRFMPIWISAKFENAKSK"
     gene            complement(2942..3018)
                     /locus_tag="PLF77_07675"
     tRNA            complement(2942..3018)
                     /locus_tag="PLF77_07675"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2982..2984),aa:Met,seq:cat)
     gene            complement(3090..4784)
                     /locus_tag="PLF77_07680"
     CDS             complement(3090..4784)
                     /locus_tag="PLF77_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPC08771.1"
                     /translation="MRKALVYIICLTLISLGTGTLWAKDKYTVAILPFAIHSAENIDY
                     IKQGVEEMLTARISAAEKITVIKKADVRQELQNAQIKDINSQNVLEIGKKFTADYVVW
                     GSITKIGNSISLDGKLTNIEQAKSDLGISSQSSSLDDVIPKINDFSENIISHILGTAP
                     PAQTAVSETSPGPSAASRESQIIAGMRSGGRKAGTLTSVINAEFINAPSPFKRQGFWM
                     SQKIPTELKGMDIGDVNNDGANEIVLIDKNAVYIYQKTQDDLKLLETIKGPSYETYRA
                     VDIADINRNGINEIFVTVTQDTFVKSFVLEFKDGKYEKIASGIRLFLRVIDTPSGMPL
                     LLGQELGFGKPFDTPIYEVIWKDKAYVTDQKMQIPQGLSVYGLNIDNVGTGGGGEKIL
                     ALDDLDYLYILDKTTKSLPSLTSFGFASEKIIWRSEDVYGGSNNFIENLDKNNPDDPR
                     AKSTYINLRILTADTNNDGKKEIIIVKNLSSTARLLKQLKLFTASEIYNLEWDGLGMS
                     ENWRTKKINGYVADYAIKDVDNDGRHEIVLALVMSVGVSLRDRSVLVVYELDAPQQ"
     gene            complement(4871..5533)
                     /locus_tag="PLF77_07685"
     CDS             complement(4871..5533)
                     /locus_tag="PLF77_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPC08772.1"
                     /translation="MTETQTAENYTPLEEIFNSLTHGIGILISLAGMVLMIVFASRYG
                     NVNHIVSCTIFGLTLVMLYTASTLYHSFRKPTLKHVFKICDHSCIYLLIAGTYTPFLM
                     VTLKGMLGWSLFAVVWTLTAAGILFKIFFVYRFNILSTIAYILMGWIIIFAVKPLLHS
                     LPEGGIAFLVAGGLAYTLGTIFYAWKKLPFNHAIWHLFVLGGSICHFLAVILYVIPGP
                     SI"
     gene            5716..7017
                     /gene="purB"
                     /locus_tag="PLF77_07690"
     CDS             5716..7017
                     /gene="purB"
                     /locus_tag="PLF77_07690"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPC08773.1"
                     /translation="MIPRYSRSVMSEVWSQENKYRKWLDVEMLACEAMARLGKIPQKS
                     LRNIKNKVAIRTERIDEIEKTTRHDVIAFLTSLTEAVGEDGRFIHMGMTSSDVLDTAF
                     AVQLKEAAELLLEDIDVLLLVLKKRAMEHKNTLMIGRSHGIHAEPITFGLKMALWYQE
                     MQRNRKRLLQAKESVSYGKVSGAVGTFSFIDPSVEDYVCNKLGLKPAPVSSQIVQRDR
                     HAEFFTALAIIASSLDKFAQEIRLLQRTEVREAEEYFSPGQKGSSAMPHKRNPVLSEN
                     ISGLSRLMRSYAVAALEDVALWHERDISHSSVERVIGPDATIALDFMLSRFTNMMDKL
                     VVYPERMMANLNMTHGIIFSQMVLLALVEKGLTREASYTIVQKHAMRSWTEGLEFRVL
                     LEKDAAVKKYLSKKDLAAIFNLKNFLNHLDFIFERVFKAKF"
     gene            complement(7030..8619)
                     /gene="nadB"
                     /locus_tag="PLF77_07695"
     CDS             complement(7030..8619)
                     /gene="nadB"
                     /locus_tag="PLF77_07695"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HPC08774.1"
                     /translation="MEFKTDFLIIGSGIAGLSLAIKAAQLGSVTVVTKKEKFESNTNY
                     AQGGIAAVTDKTDSFAEHIQDTLTCGAGLCNRDVVEFIVREAPPRIRELIDWGVHFTR
                     SATEPALYDLGREGGHNRRRVLHARDFTGQEIERALNEQVSSLPNVLIHENHIGIDLI
                     IGKKEAGQPERCLGAYVLDINQGEVHTYRAKYTILCTGGAGKVYLITTNPDIATGDGL
                     AMAYRAGARIANMEFIQFHPTCLYHPSAKAFLISEAVRGEGGILRLKNGSTFMEKYHP
                     MNSLAPRDIVAKAIDSEIKKSGDEYVLLDVTHLKKDFIVNRFPNIYQRCLEYGIDMAT
                     DPIPIVPAAHYICGGVAVDHYGRTSLPRLFACGEVSCTGLHGANRLASNSLLEAVVYA
                     HRVFTKIAETFKDANHDTSPIALWDSSGTTESDDSVVVTNNWDEIRRCMWNYVGIVRS
                     NKRLERAARRIKLIQNEIHDYYWNFKVTKDLIELRNITTVAQLIVESASSRKESRGLH
                     YNIDYPETRDDFRHDTLLIKS"
     gene            8727..9722
                     /gene="tsaD"
                     /locus_tag="PLF77_07700"
     CDS             8727..9722
                     /gene="tsaD"
                     /locus_tag="PLF77_07700"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPC08775.1"
                     /translation="MVVLGIESSCDETAAAVVKNGRLLSNVIASQIDIHSPYGGVVPE
                     IASRKHLEVIHPVIRQALNDAGIALQEIEGIAVTCGPGLIGSLLIGLSAAKAMAMALD
                     VPMIGVNHLEGHIAAVFVSGDAPPFPFIALVVSGGHTSVYRVENFRDFRLIGQTRDDA
                     AGEAFDKAAKLLNLGYPGGVVIDRMAKKGNPQAVAFPRAMKDSPDFSFSGLKTSLLTM
                     LKKRGRDFDQDELPDIAASYQEAIVDTLVEKTVRAAEENAIDQIVVCGGVAANSRLRE
                     KFNQAAAEKKMTLFVPPIVLCTDNAAMVAAIGEIMLNNGLRDSFDLNAVSRWPLS"
     gene            9694..10602
                     /gene="rsmA"
                     /locus_tag="PLF77_07705"
     CDS             9694..10602
                     /gene="rsmA"
                     /locus_tag="PLF77_07705"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPC08776.1"
                     /translation="MPFRAGPYRDSGALKDKTIMLLPRDILHRYSIKPRKSLGQCFLV
                     DQDRLAQIVQLADAGKDDVVVEIGAGIGVLTQSLAERAARVIAVELDDELVKVLEDRL
                     HSFPNIDIHHGNILRFDFRSLFVRTHRKIKVVGNIPYNITSPLLFYLLSCRDVIHDFV
                     LMMQKEMVERLTASPGEKAYGVPSVILQMFADMENVLTIPPSCFYPRPKVESAVLKGA
                     FGDQPLFAIQDEDFFIRLVRDAFGQRRKTLMNNLKQASWMRKDEALLKDILKEAAIDG
                     QRRGETLSVKEFAVLSNVIKDTLKIT"
     gene            complement(10645..11274)
                     /locus_tag="PLF77_07710"
     CDS             complement(10645..11274)
                     /locus_tag="PLF77_07710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08777.1"
                     /translation="MKKILQIFLITAIALVSFNVFAATSSISDNELDTICAQAGAITV
                     RIGTFTVESQSLKNIATDGWNYWDPDHDSANLFKDPHPNNPPGFFDGSSTTNPQKNPG
                     AAGYNQVGYFGYDEAYLFGGTVTNTGYMMLEVVSTSDPRVNDGCKLEVIMRNQSVDAQ
                     IGFQMVMKLSDSPDLSGDQSLGRVYTQGIKMTNNGHLTVFAHNTPGPSF"
     gene            11572..12903
                     /gene="miaB"
                     /locus_tag="PLF77_07715"
     CDS             11572..12903
                     /gene="miaB"
                     /locus_tag="PLF77_07715"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPC08778.1"
                     /translation="MKNKLLYIQTFGCQMNVHDSEQIVSLLKASGYQMTENEKLADLI
                     MLNTCSIREKAAQKVYSQLGRLRHLKKQNPDLIIGVAGCLAQHLGNKFHKRMDYLDFV
                     CGTHNIHQLPEMIDTVVRDRRKITRIDFHPSLSSMSIFVPPRDGQISAFVTIMQGCNN
                     FCAYCVVPYLRGREISRTPADVLDEIGRLADAGVMEVTLLGQNVNSYGKTLHDGSNFA
                     SLIKKIGDIEGIKRIRFTTSHPRDLSGEMIDCFRTEEKLCAHIHLPVQSGSNHVLALM
                     NRGYTVEDYMQKVDALRRTRPDISITSDMIVGFPGETEKDYQETIDMMEKIRFDSTFS
                     FKYSEREGTAAAKLKDSVPEGEKLRRLAALQDLQNRHTLEKNTQLLGTRQQVLVEGRS
                     KNTANDLTGRTGSWKIVNFAGPSELKGQTVVVGITEAYVHSLRGRIEKTEQ"
     gene            12916..13416
                     /locus_tag="PLF77_07720"
     CDS             12916..13416
                     /locus_tag="PLF77_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HPC08779.1"
                     /translation="MLVEMKVLGLTIDPVTNTPIVILKDMDNNKAIPIWIGLFEASAI
                     ATEMKKIAFPRPLTHDLFVEFLRKFDIAVNRIEIVDIRNNTFFANIYLMKEGLDYAID
                     ARPSDAIAIALRTNAPIFVDETVIEKSRNVDFGVKLGDLDKMKDEKLKEFLENLSMED
                     FGKYKM"
BASE COUNT         3144 a         3097 c         3773 g         3448 t
ORIGIN      
        1 agtttcagac aggcgtccac cactttttca tcgtaaagtt tccccctgtt tttttcaatt
       61 tccttcaatg caaattccag ccccagggaa ggacggtagg gacgatgcga tgcgatcgct
      121 tccacaacat cggccacagc catgatgcgc gcttcgatca gaatgtcgtt ccctttcagg
      181 ttgcggggat aaccggagcc gtccatgcgt tcatgatgct ggtagacgat ttctgaaagc
      241 ggccaggggg attccacatc tttgagcatc tcgtatccgc tttccggatg tgttttgatc
      301 atggagaatt caatattggt caatcctgtc ggtttgctca gtatttctgc cgggatggat
      361 aattttccga tatcgtgaat gatgccggcc atattcaatc cttcgatgat atccttgtcc
      421 agccccattt ctgcggcgat ggcacaggcc agatttgccg tgcgggactg atggcctgcc
      481 gtgtaggggt cccgcgcttc cagggcggaa atgagcacct gaatggtggt gccgacggcc
      541 tttttcaggc tttccagggt ctgttgcagc ttgtcttcgg ccagtttgcg ctcggtaatg
      601 tttctggatt ctcccagaac cgacaagggg tttccctgtt cgtcccggat taaactgaat
      661 tcgcattctc cccagacggt ctggccgttt ttaaggatga attccagttc aatggttttg
      721 aaaaggcgat tgccggatgt ggatttgagc gccttgggca attccgcggc aatgaaatcc
      781 gaaacccgat caaacgattc tttcgtcagg atccgcttcc acggcatgtt gatgatttca
      841 tcggcggaat atccggtaat gcgttccacg gaagggctga cgtaggtgat gtgcatattc
      901 aaatccatga gccagacctg atctttcatg tggtcggcga ggagacggta cttctcttcg
      961 ctttttttca atgcttcttc cgattgcttg cggtcggtga tgtcgcggag gttgacgatg
     1021 gcggcttcaa taatattatt acgaatcaga ttgctgccaa tcgcttcaaa cgtgcgccag
     1081 cttccatccc ggtggcgaag ccggatttca cttctttgga caggcgcgtt tgtatcgcta
     1141 aacagcaaat tgaatgcccg gcttatggtt tcaagatcat ccggatggag aaactcaaaa
     1201 gcatgattgc ccagcctttc ttcaacgcta aatcccagaa tgctgacgga cggattttca
     1261 tacgttacga ttccctcccg gttgaccatg ataatgatgt ccgaggactg atccgccagg
     1321 gcccggaacc gctgttcttc ttccagcagt ttatcctgta catttttgcg gtctgtaata
     1381 tccacggttt ctcccatgat ggatacgggt ttgccgtttt cgtcgcggat gaaactcagg
     1441 tgattttcaa tccacaggag gtggccgtct gcgtggatgg cctgaagttc cagaacaaaa
     1501 cggggtttgt cgggagaagg ggaggcggcc attgctttgg gtaattccgt ggcaaaagta
     1561 tcgcgcagct tttgataaga ctcttttgtg aaaagattcg ttaatttgag cgttttgatt
     1621 ttctcgagag ggtatccgta tattttttcc accgaggggc tgatataggt gaattttaag
     1681 ttaaggtcca tgatccagat gtgttcggtg atgttgtcgg ccagcagacg gtataacgcc
     1741 tcgctgttgg ccagggcttc ttccgctttc ctgcggtcgg taatgtcgcg gagattgaca
     1801 agcacgacgt cctgcatgcc gtcctgcacc acccgggtgc cgacagcttc cagtgtccgg
     1861 acggttttat ctttggcgat ggttcgtatt tctgtacgat ggagttcggc atcgggatta
     1921 ttcaagagct cattaaatac ccgggccgtg ggggcccggt catcgccatg gatgaatgtg
     1981 taaatttcat tgccgatgat gtcctctttt ttaaaatcca tcactttttc cgcccgggta
     2041 ttggcgtagg tgatgattcc ctgacggttg atcagcaaaa tgatgtccag agactgttca
     2101 atcaacgtgc ggaaaagctg ttcgtcgtag cgaatgattt tgaggtcttt cttccccggg
     2161 gtttgcggat gtttcggcgt cgcggcgcct cgggggcagg aattgttttt catgttcaaa
     2221 cctcaaccgg agaagatcga tccgaccatc gatcgatacc gtaaaaagcg gtcggacggt
     2281 atccgcttat ttggattttg cgttttcaaa ttttgcagaa atccatatgg gcatgaagcg
     2341 tgaagaattt tgtttccagg agatatagtc ctcgattttt ttgatcgctt ccttcggggt
     2401 gttgacaaat tcgacccccg cttcatacca gttatcttca aaaaccacct tgatccattt
     2461 gactttcccc tgggcggtga ttttttcctg caatgtcttt aaggtgaaat cgattttgag
     2521 aagggtatcg acgggcagga gaatgttgcc tctgattttt gcgccggtgg ccgatatgtc
     2581 ttcgctgtac ttgtagacga ggtctttctc cggaagatta gggtcgccgg ggatgaccga
     2641 tacggtgatt tcattgaatt catgaagccg ttttgatttt cttttttctt tcatggcaat
     2701 ctcattcgta aaaaactctt tgatgaaaac aggttacccc tgtgccttat cgtataaaat
     2761 ccggcataaa aatgtcaagg tattattgct ttttaaaaat cagtgcgcgg tcgtttacgg
     2821 cagctttgcg ggatctcaaa tgtaaggagt tgccggaggg ttggaaaagg acgcatcatt
     2881 caggacgacg caatatttcg ggaggacaaa gaagacaaaa aaatgaagaa ctactttaaa
     2941 atggtggcgg tgcctggatt tgaaccaggg acactacgga tatgagccgt atgctctgac
     3001 caactgagct acaccgccat gattttcgaa agagcctgac ctcaaatcag gtggcacgat
     3061 atatcgaaga gcgaatcatt tgtcaatcat tattgttgcg gcgcgtccag ctcgtataca
     3121 accaaaacgc ttctgtcgcg cagcgaaacg ccgaccgaca tgaccagcgc cagaacgatc
     3181 tcatggcggc cgtcgttatc cacgtctttg atcgcgtagt cggctacgta tccgttgatt
     3241 tttttcgtgc gccagttttc cgacatgccc agtccgtccc attccagatt gtatatttcg
     3301 ctggcggtga aaagctttaa ttgttttaaa agccgcgcgg tcgaggagag gtttttcacg
     3361 atgatgattt ctttttttcc gtcattgttg gtgtccgcag tgagaatccg caggttgatg
     3421 taggtgcttt ttgcccgggg atcgtcaggg ttgtttttgt cgagattttc aatgaagttg
     3481 ttgctgccgc cgtaaacgtc ttcgctgcgc cagatgattt tttcgctggc aaagccgaag
     3541 gaggtcagcg acggcagaga tttggttgtc ttgtcgagaa tgtacagata gtcgaggtcg
     3601 tccagcgcca ggattttttc gccgccgccg ccggtgccga cattgtcgat gttcagcccg
     3661 taaaccgata agccctgggg aatctgcatt ttttgatcgg tgacataggc tttgtctttc
     3721 cagattactt catagatcgg ggtgtcgaac ggcttgccga atcccagttc ctgaccgagc
     3781 aggaggggca ttccggaagg cgtatcgatg acccgcaaaa acaggcggat gccggaggca
     3841 atcttttcat atttgccgtc tttgaattcc aggacaaagg actttacgaa tgtgtcctgt
     3901 gtgacggtga cgaatatttc gttgatgccg tttcggttga tgtcggcaat atccacggcc
     3961 cgataagttt cataggaagg tcccttgatg gtttccagca gtttcagatc gtcctgggtt
     4021 ttctggtaga tgtaaacggc gtttttatcg atgagcacaa tttcattggc gccgtcgttg
     4081 ttgacgtctc cgatatccat tcccttgagt tcggtcggaa ttttctggct catccagaag
     4141 ccctgacgct tgaacggcga gggtgcattg atgaattcgg cgttgataac ggaagtcagg
     4201 gtgccggctt ttcgtccgcc ggatcgcatg ccggcgataa tctgggattc ccgggaagcg
     4261 gccgaggggc cgggcgatgt ttcagagacg gcggtttgcg ccggcggcgc cgtgcccaga
     4321 atgtgactga taatgttttc ggaaaaatcg ttgatcttcg gaatgacatc atccagcgat
     4381 gacgactggg atgaaatgcc caggtcggac ttcgcctgtt cgatgttcgt cagtttcccg
     4441 tccaggctga tgctgtttcc gattttggtg atgctgcccc agacgacata gtcggcggtg
     4501 aattttttgc cgatctccag gacgttctgg ctgttgatat ccttgatttg ggcgttttga
     4561 agttcctgcc ggacatccgc ttttttgatc acggtgattt tttcggcggc ggagatgcgg
     4621 gcggtcagca tttcttcgac gccctgtttg atgtaatcga tgttttccgc gctgtgaatg
     4681 gcaaagggaa gaatggcgac ggtgtattta tctttggccc agagcgtgcc ggttccgagg
     4741 gaaatcagcg tcaggcaaat aatatatact aaagcttttc gcatggttat ctcctttggg
     4801 atgttatgga aatttacagc agggatgttc tagcatttca gacgctttaa ttcaaggctg
     4861 atttagggga ttaaatcgac ggcccgggaa tcacataaag aataacggcc agaaaatggc
     4921 agatgcttcc gcccaaaacg aacaggtgcc agatggcgtg gttgaaaggg agtttcttcc
     4981 aggcgtaaaa aatggtgccc agcgtatagg ccagtccgcc ggcaaccaga aaagcgatgc
     5041 cgccttccgg cagggagtgt aatagaggtt tgacggcgaa gatgatgatc cagcccatta
     5101 aaatataggc gatggtggaa agaatattga aacgatagac gaaaaatatc ttgaataaga
     5161 taccggctgc ggtcagcgtc cagaccaccg caaacaggct ccagcccagc atgcctttga
     5221 gcgtgaccat cagaaacggt gtgtaggttc cggcaatcag aagatagatg caggaatgat
     5281 cgcatatttt aaagacatgc tttaacgtcg gtttccggaa gctgtgataa agggtggagg
     5341 ctgtgtagag catgaccagc gtcaggccga aaatggtgca actgacaata tggttcacgt
     5401 ttccgtaacg gctggcaaaa acgatcatca acaccatgcc ggccaggctg atgagaatcc
     5461 cgatgccgtg ggtgaggctg ttgaagattt cttccagcgg ggtgtaattt tcggctgtct
     5521 gtgtttcggt cataaatatt gattcccgtt tacgtggatt gtctcatatg ctcatccggg
     5581 ggggggttgt caaccggtaa tacccgatgc gtgcaagaat agtcttgacg gatgggcaaa
     5641 agtcgtaata ttactgcccc aaagaaatga aaatgctgtc tttaaaataa atcaagccat
     5701 ccaaaggaga cggacgtgat accaagatat tcccgaagtg taatgagcga ggtgtggagt
     5761 caggaaaaca aataccggaa atggctggat gtggaaatgc tggcctgcga agctatggcc
     5821 aggctgggaa agattcctca aaaatcactc cggaatatca agaataaggt tgccatccgc
     5881 acggaacgta tcgatgaaat cgaaaagacc acacggcacg atgtgatcgc ctttctgacc
     5941 tccctgacgg aagccgtcgg cgaggacgga cgtttcattc atatgggcat gacctcgtcg
     6001 gatgtgctcg ataccgcgtt tgccgttcag ttgaaagagg ccgcggagct tctgctggaa
     6061 gatatcgatg tgctgctgct ggtgctcaag aagcgggcga tggaacataa gaacacgctg
     6121 atgatcggcc ggtcgcacgg aattcacgcg gaaccgatta cgttcgggct gaaaatggcg
     6181 ctgtggtatc aggaaatgca gcgcaaccgc aaacgcctgc tgcaggcgaa agaatcggtc
     6241 agctacggca aggtctcggg cgcggtcggc acgttttcgt ttatcgatcc ttccgtggag
     6301 gactacgtct gcaacaagct cggtctgaaa ccggcgcccg tgtcgtcgca gatcgtgcag
     6361 cgggaccgtc atgcggaatt tttcacggcg ctggcgatta tcgcgtcgtc cctggacaag
     6421 ttcgcgcagg agatccgtct gctgcagcgg acggaggtgc gggaggccga agagtatttt
     6481 tctcccggac agaagggatc ttccgccatg ccgcataagc gcaatccggt tctttcggaa
     6541 aacatatcgg gtctgtccag gctgatgagg tcttacgcgg tggcggcgct ggaggatgtc
     6601 gcgctgtggc acgaacggga tatcagccat tcgtccgtgg agcgggtcat cggaccggac
     6661 gcgacgatcg cgctggattt tatgctcagc cgcttcacca atatgatgga caaactggtg
     6721 gtttatcccg aacggatgat ggccaatctg aatatgacgc acggcattat cttttcgcag
     6781 atggtgctgt tggcgctggt ggaaaagggg ctgacgcgcg aagcctctta tacgatcgtg
     6841 cagaagcacg ccatgcggtc ctggacggaa gggctggaat tcagagtgct gctggaaaaa
     6901 gacgccgcgg taaaaaaata tttgagcaaa aaagatctgg cggccatttt caatctgaag
     6961 aattttttaa accatctgga ttttatcttc gagagggtct tcaaagcaaa attctaggct
     7021 gttgtctttt cagcttttga tcagaagggt gtcgtgccgg aagtcgtcgc gtgtttcggg
     7081 ataatcgata ttatagtgca gtccgcggct ttcttttctt gacgatgcgc tttccacgat
     7141 cagttgcgcc acggtggtga tgttgcgcag ttcgatcagg tctttggtga ctttgaaatt
     7201 ccagtaataa tcgtgaattt cattctgaat gagtttgatt ctccgcgccg cccgctccag
     7261 ccgtttattc gagcggacga tcccgacgta attccacatg caccgcctga tttcatccca
     7321 gttgttggtg acgacgacgc tgtcgtcgct ttcggtggtg ccgctggaat cccacaacgc
     7381 gatgggcgag gtgtcgtggt tggcgtcctt gaaggtttcg gcgatctttg tgaagacgcg
     7441 atgcgcataa accacggcct ccagcaggga attgctggcc agccggttcg cgccgtgcag
     7501 tccggtgcag gacacttcgc cgcaggcgaa aagacggggg agggaggtcc ggccgtaatg
     7561 atcgactgcc acgccgccgc agatgtaatg cgccgccgga acaatgggga tgggatcggt
     7621 cgccatgtcg atgccgtact ccaggcagcg ctgataaata ttggggaagc ggttgacgat
     7681 gaaatctttt ttcagatgcg tcacatccag cagcacgtac tcgtcgccgg attttttgat
     7741 ttccgagtcg atggctttgg ccacgatgtc ccgcggggcc aggctgttca tgggatgata
     7801 tttttccatg aacgtcgatc cgtttttcaa ccgtaaaatg ccgccttcgc cacggacggc
     7861 ctcgctgatg aggaaggcct tggccgaggg atggtaaaga caggtgggat gaaactggat
     7921 gaattccatg ttggcaattc tggcgccggc ccggtaggcc atggccaggc cgtctccggt
     7981 ggcgatatcg gggttggtgg tgatgaggta caccttgccc gcgcctcccg tgcacaggat
     8041 ggtgtatttg gcgcgatagg tatgcacttc gccctggttg atatccagaa cgtatgcgcc
     8101 gaggcagcgt tcgggctggc cggcttcttt tttgccgatg atcagatcga tgccgatgtg
     8161 gttttcatga atcaggacgt tgggcagact ggagacctgt tcgttgagcg cgcgttcgat
     8221 ttcctgaccg gtgaaatccc tggcatgcag cacacgccgg cgattgtggc cgccttcccg
     8281 tccgagatcg tacagggcgg gctcggttgc cgagcgggtg aagtgcacgc cccagtcgat
     8341 gagttcccgg attcgcggcg gcgcttcccg gacgatgaac tccacgacgt cccggttgca
     8401 aagccccgcg ccgcaggtca gcgtgtcctg aatgtgttcg gcaaagctgt cggttttatc
     8461 ggtcacggcg gcaatgccgc cctgggcgta attggtgttg gattcgaact tttctttttt
     8521 ggtgacgacg gtgacggaac ccagctgcgc cgccttgatg gcgaggctca ggccggcaat
     8581 accgctgccg atgattaaaa agtccgtttt gaattccatt gctgttttcc gctacaaaac
     8641 catcgccaaa ttttgtttac aaaagctgtt tgcttctata taggtaattg cggattttgg
     8701 caaagagaaa atacaagagg ttttacatgg tcgtgctggg aatcgaatcc tcctgcgatg
     8761 aaaccgcggc ggcggttgtg aaaaacggca ggctcctgtc caatgtgatt gcatcgcaga
     8821 tcgacattca ttcgccgtac ggcggcgttg tgccggagat tgcctcgcgc aagcatctgg
     8881 aggttatcca tccggttatc cgccaggcct taaacgacgc cggaatcgcg ttgcaggaga
     8941 tcgaggggat tgcggtcacc tgcgggccgg ggctgatcgg atcgctgctg atcggcctgt
     9001 ccgcggccaa agcgatggcg atggcgctgg atgttcccat gatcggcgtc aatcatctgg
     9061 agggacatat tgccgcggtt tttgtttccg gagacgctcc gccgtttccg tttattgccc
     9121 tggtggtttc gggagggcac accagcgttt accgggtcga aaatttccgc gatttccggc
     9181 tgatcggaca gacacgcgac gacgccgcag gcgaagcctt cgacaaagcg gccaagctgc
     9241 tcaacctggg atatccgggc ggcgttgtga tcgaccggat ggccaagaag ggaaatccgc
     9301 aggcggtggc tttcccgcgg gcgatgaagg attccccgga tttcagtttc agcggtttga
     9361 aaacgtcgct tctgaccatg ctgaaaaagc gcggacgcga ttttgatcaa gacgaactgc
     9421 cggatattgc ggcaagttat caggaagcga ttgtcgatac gctggtggag aaaaccgtga
     9481 gagccgcgga ggaaaacgca attgaccaaa ttgtggtgtg cggcggcgtg gcggccaaca
     9541 gccgcctgcg cgaaaagttc aatcaagctg cagcggaaaa aaagatgacg ctgtttgtgc
     9601 cgccgattgt cttgtgtacg gacaacgcgg ccatggtggc ggcaatcggc gaaatcatgc
     9661 tgaacaacgg attacgggat tcgtttgatc tgaatgccgt ttcgcgctgg cccttatcgt
     9721 gattccggcg ccctgaaaga caaaaccatc atgcttttac cgagagacat tcttcatcgt
     9781 tattccatca aaccgagaaa aagcctggga caatgctttc ttgtcgatca ggaccgtctg
     9841 gcgcagattg tacaactggc cgatgcgggg aaggatgatg tggtcgtgga aatcggcgcg
     9901 ggcatcggtg ttctgacgca gtcgctggcc gaaagagcgg cccgcgttat tgccgtggag
     9961 ctcgacgatg aactggtgaa ggtactggaa gaccggctgc attctttccc gaacatcgac
    10021 attcatcacg gcaatatcct gcgttttgat ttccgttccc ttttcgtccg gacgcaccgg
    10081 aaaatcaagg ttgtcggcaa tattccttat aatattacca gtccgctttt gttctatctg
    10141 ttgagttgcc gggatgtcat tcatgatttt gtactcatga tgcagaagga aatggttgaa
    10201 cgtctgacgg cgagccccgg cgaaaaagct tacggtgtcc cttccgtcat tctgcaaatg
    10261 tttgcggaca tggaaaacgt tctgacgatt ccgccctcct gtttctatcc gcgtcccaaa
    10321 gtggaatccg cagtgctcaa gggggcgttt ggcgatcaac cgctcttcgc aattcaggat
    10381 gaagattttt tcatcaggct tgttcgggat gcgttcggac aacggcgaaa aacgctgatg
    10441 aataatctga aacaggcatc gtggatgcgg aaggatgaag ccttgttgaa agacattctg
    10501 aaggaagccg ccattgacgg tcagcgaaga ggggagacgc tatccgtgaa agaatttgcc
    10561 gtcctgagca atgtgataaa agatacactc aaaataacct gaaggatcag gtgctgaaat
    10621 tcgatacgaa tcgtcggaaa cgttttagaa gctggggccc ggcgtgttat gagcgaaaac
    10681 ggtcagatgg ccgttgttgg tcatcttgat gccttgcgtg taaacacggc ccagggactg
    10741 atcgcccgag aggtcggggg aatcgctgag tttcataacc atctggaatc cgatttgcgc
    10801 atccacggac tggtttctca tgatgacttc aagcttgcat ccgtcgttga ccctggggtc
    10861 gctggtggat acgacttcaa gcatcatgta gccggtgttg gtgaccgtgc cgccaaagag
    10921 ataagcctca tcatatccga agtaaccgac ctgattgtag cctgccgcac cgggattttt
    10981 ctgaggattg gtggtggatg atccgtcaaa aaatccggga gggttgttcg gatgcggatc
    11041 tttgaataga ttagccgaat cgtgatccgg atcccagtaa ttccatccgt cggtggcaat
    11101 gtttttcagg ctctggcttt caacggtaaa ggtgcctatg cgcacggtga tggctccggc
    11161 ctgcgcgcag atggtgtcaa gttcgttgtc ggaaatggaa gacgtagcag caaaaacatt
    11221 gaaagagacc agggcaatag ctgtaatcag gaaaatttgt aaaatttttt tcatgtattc
    11281 tctctgaatc aatacattaa tttttttact gctatttcaa agattatttt gagttgctga
    11341 ttttctagca caactttacc ggtctgtaaa gagatttttt taattatttt aaaatttttt
    11401 taattcttca atatgtgtag aatcgtgtag acgaaagggc tgaaacgttc aggaaagaaa
    11461 aaaagagacg acggaagaaa aattagccat tgccggtata aatagtggaa aaacattgac
    11521 taatttgcgg tgaaataata atggtacggt cgaaaacggt aaacgggaac aatgaaaaat
    11581 aaactccttt atattcaaac atttggatgc cagatgaatg ttcacgattc ggaacagatc
    11641 gtgtcattgc tcaaagcgtc gggctatcaa atgacggaaa acgaaaaact tgccgatctg
    11701 attatgctca acacctgctc catccgggaa aaggccgcgc agaaggtcta cagccaactg
    11761 ggacggttaa ggcatttaaa gaagcagaat ccggatctga tcatcggcgt ggcgggttgc
    11821 ctggcgcagc atctcggaaa caaatttcat aagagaatgg attatctgga ttttgtatgc
    11881 gggacgcata atatccatca gcttccggaa atgatcgata cggttgtccg ggacagacga
    11941 aaaatcaccc ggatcgattt tcatccgtcg ttgagctcga tgagcatttt tgttccgccc
    12001 cgggacggcc agatcagcgc ctttgtgacg attatgcagg gatgcaataa tttctgcgcg
    12061 tactgcgttg tcccttatct gcgcggacgg gagatcagcc ggacgcccgc cgacgtcctc
    12121 gatgagatcg gcagacttgc cgatgcgggg gtcatggaag tgacgctgct cggacaaaat
    12181 gtgaactctt acggcaaaac gctgcatgac ggatcgaatt ttgcgtcgct gattaaaaag
    12241 ataggcgaca tcgaaggcat caagcggata cggtttacga cgtcgcatcc gcgcgatctg
    12301 tccggcgaaa tgatcgattg tttcaggacg gaagaaaaac tctgcgcaca cattcatctt
    12361 ccggttcagt ccggttccaa tcacgttctg gcgctgatga accggggata tacggttgag
    12421 gattatatgc agaaagtgga tgctttgcgc aggaccaggc ccgacatcag cattacctcg
    12481 gatatgatcg tcggatttcc gggagaaacc gaaaaagatt accaagaaac aattgacatg
    12541 atggaaaaaa tcaggtttga ttcaactttc tctttcaagt attctgaaag ggaggggaca
    12601 gcggcggcaa aattaaaaga ctcggttccg gaaggcgaaa agttaagacg cctggcggcg
    12661 ctgcaggatt tgcagaatcg gcacacgctg gaaaaaaata cgcaactgct gggaacgcgg
    12721 cagcaggtgc tggtggaagg ccgaagcaaa aataccgcca atgatctgac gggacgaacg
    12781 ggatcatgga aaattgtcaa ttttgcgggc ccttcagagt tgaaaggcca aacggttgtc
    12841 gtcggcataa cggaagcgta tgtgcattct ttaagaggcc ggatagaaaa aacagaacaa
    12901 taggagagat tgtccgtgtt agttgaaatg aaggttctgg gtttgaccat cgatcccgta
    12961 accaatacgc cgattgtgat attaaaggat atggataaca acaaggccat cccgatctgg
    13021 atcggtttgt ttgaggccag cgcgattgcc acggaaatga aaaagattgc gtttcctcga
    13081 ccgctgaccc acgatctgtt tgtcgagttt ttgcgcaagt tcgatattgc cgtaaaccgg
    13141 attgaaattg tcgatatccg caacaacact tttttcgcaa atatttattt gatgaaagag
    13201 gggctggatt atgccattga cgcacgtccc agcgatgcga tcgccatcgc cctgaggacc
    13261 aacgccccga tattcgtgga tgagacggtc atcgaaaaat cgcgcaatgt cgatttcggc
    13321 gtcaagctgg gcgatctgga taaaatgaaa gatgaaaaac tgaaagaatt tctggaaaat
    13381 ctttccatgg aggatttcgg caaatataag atgtaatgag gctcgctaaa gacgagcgca
    13441 gcgaagtctg aagcgcaagc cg
//
LOCUS       DAOOLB010000057        13137 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_5223_length_13137_cov_30.161749, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000057 DAOOLB010000000
VERSION     DAOOLB010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 13137)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13137)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13137
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5223_length_13137_cov_30.161749"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="PLF77_07725"
     CDS             complement(<1..197)
                     /locus_tag="PLF77_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08780.1"
                     /translation="MNKTIWKPEPEINLILKNYNTFAILSCGICANLSYTGGKTGVEV
                     LKAYLLKNHKQIKLDKVILAA"
     gene            complement(251..733)
                     /locus_tag="PLF77_07730"
     CDS             complement(251..733)
                     /locus_tag="PLF77_07730"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPC08781.1"
                     /translation="MKQKAFQDYYPDDLSHCYGCGRLNEHGLQIKSYWDGEESVCVYQ
                     PDPRYMAIPGMAYGGLIASLIDCHSTGTAAAAKYCSENREMDTLPPIRFLTASLHVNY
                     LLPTPLGGPIEIRGKVKEIKRRKVVIESRLIAGGQICATGEVVAIQVPEALMPDHLKK
                     "
     gene            complement(767..943)
                     /locus_tag="PLF77_07735"
     CDS             complement(767..943)
                     /locus_tag="PLF77_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08782.1"
                     /translation="MKTKFETILDVKIYTIDAVEALPYNFRSSTNVIFDNEHVHVDIA
                     TDAQKMHAFLSSRL"
     gene            complement(1038..1988)
                     /locus_tag="PLF77_07740"
     CDS             complement(1038..1988)
                     /locus_tag="PLF77_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC08783.1"
                     /translation="MTTALKAVDRVEILTLQDNYIEITAMDSNAVVTRANPLKEGEIR
                     ASVLAEHGFSALVQTTLGNKTHTLLLDFGFSENGAAQNAETLNVDMTQVEAAALSHGH
                     SDHTGGMAKLVTMTGKKNIPFAVHPSAFKSPRYLKFGEELKINFPELTREMVKAAGLT
                     VVETEKPYLLLEDTILFLGEIPRSTDFETGWPLPHCQQDGKEVWDAIEDDTSIVMNLK
                     NKGLIILSGCAHSGIINTVHHAMKVTGINKIHAVMGGFHLSGPFFEPIIDRTTQEFKK
                     INPAYIIPTHCTGRKAIMEMEKQMPEQFILNMAGTKLTFN"
     gene            complement(2009..2347)
                     /locus_tag="PLF77_07745"
     CDS             complement(2009..2347)
                     /locus_tag="PLF77_07745"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPC08784.1"
                     /translation="MKIAIPLAEGKLTAHFGHCASFAIIEIEDQTKKIIKREDIAAPP
                     HEPGLLPPWLAKQGANMIIAGGMGQRAQQLFAEQGIQVVVGAPVETPEKLITDFFAGS
                     LQVGDNLCDH"
     gene            complement(2360..3256)
                     /locus_tag="PLF77_07750"
     CDS             complement(2360..3256)
                     /locus_tag="PLF77_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPC08785.1"
                     /translation="MEEKKTCDTCGDSDCAAKKQKKDESPEEYADRRKLQATLCRIQK
                     KIVVLSGKGGVGKSTVAVNLATALALNGLRVGLLDVDIHGPSVPTMLGLENETLKGGP
                     DGLFPVEFGGLKIMSLGFLLQNPDDAVIWRGPMKMGVIKQFLTDVHWGDLDYLIVDAP
                     PGTGDEPLSLCQLIQPLDGAIIVTTPQKVAAVDVRKSISFCKRLDVPVVGVIENMSGF
                     ACPKCGEITQILPTGGGKKISTDMNVPFLGAIPMDPAIAQSGDSGKVFIYHSSKTPTG
                     EIMQKIIDEITKDLDKTKILNS"
     gene            complement(3277..3660)
                     /locus_tag="PLF77_07755"
     CDS             complement(3277..3660)
                     /locus_tag="PLF77_07755"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HPC08786.1"
                     /translation="MSELVNLDETYATAKKHADSPHNMGPMDSFSGHARITGPCGDTM
                     EFWILVEEGKIRKVSFITDGCDPSRACGSMTTCMAEGKSIEEAQAISQKDVLNVLDGL
                     PEDHEHCALLATNTLKAACEDYLNQ"
     gene            complement(3672..4553)
                     /locus_tag="PLF77_07760"
     CDS             complement(3672..4553)
                     /locus_tag="PLF77_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08787.1"
                     /translation="MKELVIISGKGGTGKTSVTASLVALAKRSVICDCDVDAADLHLV
                     LTPQIKEEHEFISGHEAVIRQTDCISCGICKDVCRFNAIHSKQNFQGELLFAVDPVAC
                     EGCKVCVEMCPEKAIDFPDCNCGKWFVSDTRLGPMVHARLGVAAENSGKLVSTVRREA
                     RAIAEKENCELIIVDGPPGIGCPVIASVTGASQVLVVTEPTVSGEHDLERVLGLTRHF
                     QIPAAVCVNKWDINPDMTERIEENAAGLGARIAGRIRYDNAVTQAQIQKKSVVEIDAP
                     SGDDIQQLWKNLNLREV"
     gene            complement(4559..5188)
                     /locus_tag="PLF77_07765"
     CDS             complement(4559..5188)
                     /locus_tag="PLF77_07765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC08788.1"
                     /translation="MQKDIIENHIKYQKRVALYRTFGYNIDLERSFIIEKSLPVAGNI
                     LEAGTGKGYFTLALAQAGYKFFSLDISVAEQRFALLNLMYYGFQSQVTFLIADVESIP
                     CNDGFFDVIFAVNMIHHLSSIRKVCDEFTRLLSPDGKIVLSDFNEKGLAILDRIHERE
                     GRYHDVSGGTLAEMKTILVERGFTVREHQSDLQDMLVAGRTNLSPDIEE"
     gene            complement(5204..6154)
                     /locus_tag="PLF77_07770"
     CDS             complement(5204..6154)
                     /locus_tag="PLF77_07770"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08789.1"
                     /translation="MSERVYQYVYGPVPSRRLGKSLGVDIVPYKTCTYDCIYCQLGKT
                     TNKTTVRLDYVPLADVLSEIEEKLLTKFECNYITVAGSGEPTLHASIGEIIGKIKERT
                     NIPVAVLTNGSLLYLPEVRQSLMKADIVIPSLDAGNEQLFQYVNRPHKDISFERVIQG
                     LIDFSKQFTGHIWLEILLLSGINGMAEEVKEMAAWVNKIHIEKVQINTVSRPAYEDFA
                     HPVDVRQLQKLARLFLCPVDILQNATSSDLSGINGVESSDKDILDLLARRPCTLEGIS
                     TGMGLHPHDVAKKLKSLLDKRLIIAMRAEQCLFYKIAERK"
     gene            complement(6156..6413)
                     /locus_tag="PLF77_07775"
     CDS             complement(6156..6413)
                     /locus_tag="PLF77_07775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPC08790.1"
                     /translation="MPTYEYECSKCGLRFERQQSMKDAPIRICPECRADVQRIVSGGS
                     GFIMKSAGVSMRDTAKSCSFEETGTTCCGAKQRCGQAPCGD"
     gene            complement(6420..7262)
                     /locus_tag="PLF77_07780"
     CDS             complement(6420..7262)
                     /locus_tag="PLF77_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08791.1"
                     /translation="MKLAVASGKGGTGKTTVSVNLARVFKRDVCLADCDVEEPNAHLF
                     LKTSGHSEKTIGLPVPDVNEELCNGCGECAKICQFSAIVTFGTTPLVFPEMCHGCGGC
                     TLVCPTKAISEKNRRIGVVETSRTENITLVTGKLNVGVALAPPLIRAVKNTLPVDAPA
                     ILDAPPGTSCPVIAAIRDSDFVLLVTEPTPFGLHDLSLAVDMVRELNLPFGVLVNRMG
                     SGDNRVHEFCRKENVPILMEIPDDRQIAENYSRGILMVDALPQYRERFENLLEKIANS
                     KKKG"
     gene            complement(7259..7873)
                     /locus_tag="PLF77_07785"
     CDS             complement(7259..7873)
                     /locus_tag="PLF77_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08792.1"
                     /translation="MKAGMIAQELKLHAPFTMFGTVTGIVIMAAFIQFQVPREFSSGL
                     FWTMHPLHVLLSALVTTAMYRMHSRGNILQIIIIGYIGSIGIATFSDSFIPYIGEWLL
                     ELPHRGVHLGFIEKWWLVNPLALAGIALGFVISHTRIPHAGHVLLSTWASLFHVLMAL
                     GTSVDVVTTILIAFFLFLAVWVPCCTSDIVFPLLWINPKESKIL"
     gene            complement(8013..8504)
                     /gene="tsaA"
                     /locus_tag="PLF77_07790"
     CDS             complement(8013..8504)
                     /gene="tsaA"
                     /locus_tag="PLF77_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HPC08793.1"
                     /translation="MINRQIIYHPIGIIHSEHTVAEKTPIQPVYACGCKGRIEVFEKY
                     ISGLSDLDGFSHVYLIYHFHQAPLVQLKVKPFLQDVERGIFSTRAPGRPNPLGLSVVE
                     LIRIEDNILYVDGIDILDGTPLLDIKPYTAKFDLHDIRKNGWQDEVDESTAAKRGKRG
                     YRS"
     gene            complement(8501..8863)
                     /locus_tag="PLF77_07795"
     CDS             complement(8501..8863)
                     /locus_tag="PLF77_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPC08794.1"
                     /translation="MKIALTTSGVDLNAPLDSRFGRAPKFLIYDQAANTFEIVDNEQN
                     LNAAQGAGIQSAQNIARLGVKALITGHCGPKAFRTLQAAGIRIYNTSAATVSEALEQY
                     REGTLTEAKSADVDGHWV"
     gene            complement(9033..9218)
                     /locus_tag="PLF77_07800"
     CDS             complement(9033..9218)
                     /locus_tag="PLF77_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08795.1"
                     /translation="MAPVLAAEVAVAVDSALATVFMPLQYRDGDEPMNTPPRLSLLIP
                     MRNNRLSERRLNFCRGN"
     gene            complement(9377..9616)
                     /locus_tag="PLF77_07805"
     CDS             complement(9377..9616)
                     /locus_tag="PLF77_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08796.1"
                     /translation="MNTNNRNPRGTGGGMSQGSGSGMGRGRGGGGRGRMGGMKAGGAL
                     GNCLCPKCGNRQPHDRGIPCTQVKCPQCGSTMIRE"
     gene            complement(9646..10194)
                     /locus_tag="PLF77_07810"
     CDS             complement(9646..10194)
                     /locus_tag="PLF77_07810"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HPC08797.1"
                     /translation="MNQYIPKEIFFTKGVGTHREQLHSFELALRDAGIEKCNLVQVSS
                     ILPPGCRIISRTEGLKKLRPGAITFCVMSRCCSNEPRRLISASVGCAIPMDKKTYGYI
                     SEHHSFGYNEKQTGDYAEDLAAAMLASTLGIDFNIDESWDEKKELFKISGKIVGTRNT
                     TQSTVLKTKDYTTVLAAAVFVF"
     gene            complement(10234..10626)
                     /locus_tag="PLF77_07815"
     CDS             complement(10234..10626)
                     /locus_tag="PLF77_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPC08798.1"
                     /translation="MKLALSVFKDCISTVFDSAEQLLIVEADTANPDKRTLQKFVATD
                     PAGRAAELKNRGVDVLICGAISRPMQALIMAQGIIVHPFVRGEVHAIITAYRNNTLGL
                     PVFSLPGCRRRAMGAGFCKQRGMRYRSR"
     gene            complement(10800..12170)
                     /locus_tag="PLF77_07820"
     CDS             complement(10800..12170)
                     /locus_tag="PLF77_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPC08799.1"
                     /translation="MKKKMPLKQGSPDIGTKQDIILDSIADGVFTVDQDWLITSFNRA
                     AEKITGVPREEAIGQRCKDVLKADICEKDCCLRKTMKTGEQIINKKVAIINAEGHKLP
                     VSISTALLRDQKGKLLGAVETFRDISVEEDLRKAVLQKYNFADILSKNHKMLQLFDIL
                     PDIAAGGSTVLIEGESGTGKELIARAIHNLSPRRKTSFVAVNCSALPDTLLESELFGY
                     KAGAFTDAKKDKPGRFKLAENGTLLLDEIGEITPAMQVKLLRVLQEKTYEPLGATQSV
                     EHNVRVIAATNKNLEDLVRQGRFREDLFYRINVFKITLPPLRERMEDIPLLIDHFISR
                     FDLLQKKEIEGVSQEALSVLMAYTYPGNIRELANIIERAFIFCKEGRIEKKHLPESLF
                     ASPPQKIRTDASSLRDVEAAWLINALQKNNWNCRETARQLGIHKSTLYRKIKALGITL
                     PGQFPL"
     gene            complement(12199..12555)
                     /locus_tag="PLF77_07825"
     CDS             complement(12199..12555)
                     /locus_tag="PLF77_07825"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPC08800.1"
                     /translation="MKIALPSYQNQIDEHFGHCEYFTVFTIDQEKKIVEEEKVSSPAG
                     CGCKSNIAEVLAAKGVKLMLAGNMGQGAVNVLNSHGIDVLRGCSGNVKTVTESWLAGT
                     LKDSGISCTQHEHGCH"
     gene            complement(12616..12909)
                     /locus_tag="PLF77_07830"
     CDS             complement(12616..12909)
                     /locus_tag="PLF77_07830"
                     /inference="COORDINATES: protein motif:HMM:NF014101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPC08801.1"
                     /translation="MPRPKCHRHIMGAPDKKYFKPRGIPSMELEEIVLNMDEFEAVRL
                     ADYEHLYQKQAAEKMNISRQTFGRIIDVAHKKIADVLINGKALRIGGDFDFDE"
     gene            complement(12950..>13137)
                     /locus_tag="PLF77_07835"
     CDS             complement(12950..>13137)
                     /locus_tag="PLF77_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08802.1"
                     /translation="RIAQEVTTAVFFSQQSNVLLRSPEEAKWYRAVGIPPTIIFSKTR
                     VNDFKYGYEEILTLDKF"
BASE COUNT         3132 a         3324 c         3038 g         3643 t
ORIGIN      
        1 caagcggcaa gaatgacttt gtccagtttg atctgtttgt gatttttcag cagatacgct
       61 ttcagaactt ccacgccggt cttgccaccc gtataggaaa gattggcaca tattccacag
      121 ctgaggatag cgaaggtgtt ataatttttt agtatgagat taatttccgg ctccggtttc
      181 caaatggttt tgttcattgt ttaccgtatc ctgactgtga aaattgtatt atataccgga
      241 ttgggatccg ctattttttt aaatggtctg gcatcagggc ttccggcacc tgaatggcaa
      301 cgacttcgcc ggtggcgcat atctggccgc cggcaatcag cctcgattcg ataacgactt
      361 tgcgcctttt gatctccttg actttccccc gtatttctat cggtccgcca agcggtgtgg
      421 gcaaaagata gtttacgtgc aaagaggcgg tcagaaaacg aatcggcggc agggtatcca
      481 tctcgcggtt ttcagagcag tatttggccg ccgccgctgt gccagtggaa tggcaatcga
      541 tcagcgacgc aatcagaccg ccgtaagcca ttccgggaat agccatgtag cggggatccg
      601 gttgataaac acagacggat tcttctccgt cccagtagct tttgatttgc agaccgtgtt
      661 cgttgaggcg accgcagccg taacagtggc ttaaatcgtc gggatagtag tcctgaaacg
      721 ctttttgctt cataaaaacc ccttttttat acaagaaatt ttatatttac aatcgagagg
      781 acagaaaagc atgcattttt tgcgcgtctg ttgcaatatc aacatgcacg tgttcattgt
      841 caaagatgac attggttgaa cttctgaaat tatacggcaa tgcctcgacg gcatcgattg
      901 tgtaaatttt tacatccaga atggtctcaa attttgtttt catctcttca gctacaccta
      961 tagccttctt gcagcttatt cagcccggat ttccgctatg aaaaaccagt aatgtcttca
     1021 tgatagtccc ctttatgtta attaaatgtc aattttgttc cggccatatt caaaataaat
     1081 tgttcgggca tttgcttttc catctccata atggctttgc gtcccgtgca gtgtgtcgga
     1141 atgatataag ccgggttgat tttcttgaat tcctgcgtgg tgcgatcaat aatcggttca
     1201 aaaaacggcc cggatagatg gaatccgccc atcactgcgt gaattttatt aataccggta
     1261 actttcatcg cgtgatgaac cgtattaatg attccggaat gcgcacaacc ggaaaggata
     1321 atcaaccctt tgtttttcag attcatgaca atagaggtgt cgtcttcgat ggcatcccaa
     1381 acttcctttc cgtcttgctg gcaatgagga agcggccatc ccgtttcgaa atccgtgcta
     1441 cggggaattt cgcccagaaa aagaatcgta tcttccagta aaagatatgg tttttcagtt
     1501 tccaccacgg tgagacccgc ggctttaacc atttcccgcg tcaattccgg aaaattgatt
     1561 tttaattcct ctccgaattt gagataacgc ggcgatttaa acgcggacgg atgcacggcg
     1621 aaagggatat tctttttacc ggtcatggtg acgagttttg ccatgccacc cgtatggtca
     1681 ctatgtccgt gggaaagagc ggctgcctcg acctgtgtca tatcaacgtt aagcgtttcc
     1741 gcattttgcg ccgcaccgtt ttccgaaaaa ccgaagtcaa gcagcagcgt atgcgttttg
     1801 tttccgagag ttgtttggac gagtgcggaa aaaccgtgct cagccaacac ggaagcccgg
     1861 atttctcctt cttttaaagg attggctcgc gtgacaacag cgttgctgtc catcgcggta
     1921 atttcaatgt agttatcctg taatgtcagg atttcaaccc tatcaactgc ttttaaagct
     1981 gttgtcatga taccaatcct cctttgattt agtgatcgca gagattgtct ccgacttgca
     2041 atgaaccggc aaaaaaatcc gtaatcagtt tttccggtgt ttcgaccgga gcaccgacta
     2101 cgacctgaat gccctgctcg gcaaacaact gttgggctcg ttgacccatg ccgccggcaa
     2161 taatcatatt agcaccttgt ttggccagcc atggcggcaa aagtcccggt tcatgaggcg
     2221 gcgcggcaat gtcttcacgt ttgatgattt ttttagtttg gtcttcgatt tcgatgatag
     2281 caaaactcgc gcagtgaccg aaatgcgctg taagttttcc ttcggctaac ggtatggcta
     2341 ttttcatgat tcattctcct tatgagttta atatttttgt cttatccaag tcttttgtaa
     2401 tttcatcaat gattttttgc atgatttccc ctgtgggcgt tttcgatgaa tgataaataa
     2461 aaaccttacc cgagtcgccg gactgtgcga ttgccggatc catgggaata gcgccaagaa
     2521 aaggcacatt catgtccgtt gagattttct tgccgccgcc ggtgggcaga atctgtgtaa
     2581 tttcaccgca tttcggacac gcaaaaccgc tcatgttttc gatgacgccg accaccggta
     2641 cgtcaagacg cttgcaaaag ctgatagatt tgcgcacgtc cactgcggcg actttctgcg
     2701 gtgtggtgac aataattgcg ccgtccagcg gctgaatcaa ctggcacaac gacaggggtt
     2761 catcacctgt gcccggagga gcgtcaacaa ttaagtaatc cagatcgccc caatgcacat
     2821 ccgtcaggaa ttgtttgata acacccattt tcatcggacc gcgccagatg acagcgtcat
     2881 cgggattctg caacaaaaac cccagagaca ttattttcag gccaccgaat tcaaccggaa
     2941 acaatccgtc ggggccgcct ttgagagttt cattttccag tcccagcatg gtgggtacgc
     3001 tgggaccgtg aatatccacg tccagcaaac cgactcgcag accgtttaat gccagcgcgg
     3061 tggccagatt gaccgcaacg gtgcttttac cgacgccgcc cttgccggaa agcacgacga
     3121 tttttttctg aatgcggcag agcgtcgcct gcaatttgcg gcgatcagcg tattcttcag
     3181 gactttcatc ctttttctgt tttttagccg cgcagtcgga atcgccgcac gtatcacatg
     3241 tttttttttc ttccactatt atatcctcca tttattttat tgattaagat aatcttcaca
     3301 agccgctttt aacgtattgg tcgccagaag cgcgcagtgt tcgtgatctt cgggaagtcc
     3361 gtcaagaaca ttgagaacat ccttttgact tattgcttgg gcttcctcaa tcgattttcc
     3421 ctcggccata caggttgtca tgctgccgca ggcacgcgaa ggatcacagc catcggtgat
     3481 aaaagagact ttcctgattt tgccttcttc aaccagaatc cagaactcca tggtgtcgcc
     3541 gcagggtccg gtaattctcg cgtgtccgct aaaagaatcc atcggtccca tattatgagg
     3601 cgaatcggca tgtttttttg ccgtggcata agtttcgtcc agatttacta actcactcat
     3661 tatttacctt cttatacctc ccgaagatta agatttttcc agagttgttg aatatcatcg
     3721 ccgcttgggg catcgatttc aacaaccgat tttttctgta tttgcgcctg tgtgacggca
     3781 ttgtcgtagc gaatccgtcc ggcaattcgc gcgcctaatc ccgcagcatt ttcttcaata
     3841 cgctcagtca tgtcgggatt gatatcccat ttgttgacgc acacagctgc tggaatttga
     3901 aaatgacggg taaggcccag caccctttcc agatcgtgtt cgcccgaaac tgttggttcc
     3961 gtgaccacca gaacctgcga agcaccggtc accgaggcaa taaccggaca gccgatgccg
     4021 ggaggaccat ccacaataat caattcacaa ttttcttttt ccgcgatggc gcgggcttcc
     4081 cgccgcacgg tggaaaccaa tttgccggag ttttcagcgg ccacccccag acgcgcgtga
     4141 accatcggcc ccagacgcgt atcggagaca aaccacttgc cgcagttgca atccgggaaa
     4201 tcaatcgctt tttccgggca catttccacg caaactttgc agccctcaca ggcaacggga
     4261 tccacggcaa ataaaagctc tccctgaaaa ttctgcttac tgtgaatggc attaaagcgg
     4321 caaacgtctt tacaaatacc gcagctgata cagtcggttt gacggataac agcttcatga
     4381 ccgctgataa attcgtgttc ttccttgatt tgcggtgtca ggactaaatg caagtcggcc
     4441 gcatccacat cgcaatcaca gatcaccgaa cgctttgcca gcgctaccag tgatgccgta
     4501 acgcttgttt tccccgtacc tccttttccg ctgattatta ccaattcctt catggccatc
     4561 attcctcaat atccggcgat aggtttgttc tgccggccac aagcatgtcc tgcaagtcgc
     4621 tttgatgttc tcttacagtg aatccgcgtt cgaccaggat ggtcttcatc tccgccaggg
     4681 tgccgccgct gacgtcgtgg tatcttcctt cgcgctcgtg aatcctgtcc aatatcgcga
     4741 gtcccttttc gttaaaatcg ctcaacacga ttttaccatc cggcgataga agtcgtgtga
     4801 attcatcaca gaccttgcgg atagaggaaa ggtgatgaat catgttcacg gcgaaaatca
     4861 catcaaaaaa accgtcgtta caggggatgg attcaacatc ggcgatgagg aaagtcacct
     4921 gcgactgaaa tccataatac atcaggttga gcagggcgaa tctctgttcc gctacggaaa
     4981 tatccaaaga gaaaaattta tatcctgcct gcgccagagc aagcgtgaaa tagcccttgc
     5041 ccgtaccggc ttccagaatg ttgcccgcta caggcaacga tttttcaatg ataaaagaac
     5101 gctccagatc gatattgtat ccgaatgtcc ggtaaagcgc gacacgtttc tgatatttta
     5161 tgtgattttc gataatgtcc ttttgcatcg tcaatccttc atcttatttt ctttccgcta
     5221 tcttgtaaaa caagcattgt tcggcgcgca ttgcaatgat gagccgctta tctaaaagac
     5281 ttttcagctt tttggccacg tcgtgcggat gcaatcccat cccggtactg attccctcga
     5341 gtgtgcatgg tcttctggcc agaagatcca gaatgtcttt gtctgaggat tccaccccat
     5401 ttattccgga aagatcagag gatgttgcgt tttggagaat gtcgaccgga cataaaaaca
     5461 aacgggcaag tttctgcaat tgtctgacgt caacgggatg ggcaaaatct tcataggctg
     5521 gacggctcac ggtattaatt tgaacttttt cgatatgaat tttattgacc catgccgcca
     5581 tttcttttac ttcttccgcc ataccgttaa ttcccgaaag caggagaatt tccagccaga
     5641 tgtgtcctgt aaattgtttt gaaaaatcaa tcagcccctg aatcactcgt tcgaaagaaa
     5701 tatctttgtg cggacgattg acgtattgga aaagttgctc attgccggca tccagcgaag
     5761 gaatgacgat atccgccttc atcaatgatt ggcggacctc cggcagataa agcagagacc
     5821 cgttggtcag aacggctacc ggaatgttcg tcctttcttt tatcttaccg atgatttcac
     5881 cgatggatgc gtgcagggtt ggttctcccg aaccggcaac ggtgatgtaa ttgcattcaa
     5941 acttcgttaa taacttttct tcgatttccg acaacacatc agccaaagga acataatcga
     6001 gccttacggt cgttttattt gtcgtttttc ccagttgaca gtagatacaa tcatacgtgc
     6061 aggttttata cggcacgatg tccactccca gcgactttcc taacctgcgg gacgggacag
     6121 gaccatagac atattgatag actctttctg acattttaat ctccacaagg ggcttgaccg
     6181 catcgttgct ttgcaccgca acaggttgtt cccgtttctt caaaggaaca gctttttgca
     6241 gtgtcgcgca tactgacacc ggctgacttc ataataaaac cactgccgcc gctaacgatt
     6301 ctttgtacgt cagctctaca ttcgggacat atcctgatag gcgcatcttt catgctttgt
     6361 tgcctttcaa aacgtagacc gcacttgctg cattcatatt cataagtcgg catttgttct
     6421 tatcctttct ttttcgaatt ggcaattttc tctaaaagat tttcaaaacg ttcccggtat
     6481 tgaggcaggg catccaccat cagaattccc cgtgaataat tttccgcaat ttgccggtca
     6541 tcgggaattt ccatcagaat gggaacgttt tcctttcgac agaattcatg aacgcggtta
     6601 tcaccgctcc ccattcgatt gacaagaacg ccaaagggca gattcaactc tctgaccata
     6661 tccaccgcca gcgataaatc gtgcaaaccg aaaggcgtcg gttccgttac cagtagaaca
     6721 aaatccgaat ccctgatcgc ggcaataacc ggacaggatg tgccgggcgg ggcatctaaa
     6781 atagccggag catcaaccgg caacgtattt tttacggcgc ggataagcgg tggagccaac
     6841 gccacgccaa catttagttt tcccgtaacc agcgtgatat tctccgtgcg cgacgtttca
     6901 acaacaccga tacgcctgtt tttttcactg atggccttag tgggacagac cagcgtgcac
     6961 ccgccgcagc cgtggcacat ttccggaaac acaagcggcg ttgttccaaa agtgacaatg
     7021 gcggagaact ggcagatttt cgcacactca ccgcagccgt tgcagagttc ttcattgaca
     7081 tccggcacgg ggaggccgat ggttttttca gaatgccccg atgtttttaa aaataagtgg
     7141 gcattgggtt cttccacgtc gcagtccgca agacacacat ctctcttaaa gacacgcgcc
     7201 agattgacgc tgaccgtggt ttttccggtc cctccttttc ctgatgcaac ggccagcttc
     7261 ataggatttt actctccttt gggttgatcc agagcagagg gaaaacgata tcgctggtgc
     7321 agcagggaac ccataccgcc agaaataaaa aaaacgcgat cagtatggtg gtaactacat
     7381 caacggacgt acccaacgcc attaaaacgt gaaataagga agcccatgta ctcagaagca
     7441 catgtcccgc atggggaatt ctggtatgcg atatgacaaa ccctaaagcg atgccggcca
     7501 gcgccagagg gttcaccagc caccattttt caatgaagcc cagatgaacg ccccgatgcg
     7561 ggagttccag cagccattcc ccgatgtagg gaataaacga atcgctgaat gtggcaatgc
     7621 cgattgagcc gatataaccg attatgatga tttgcaagat attgccgcgc gaatgcattc
     7681 tatacatggc agtggtaacc agggcgctca gcaaaacgtg taacggatgc attgtccaga
     7741 aaagcccgga ggaaaactcc cgcggcacct gaaactggat gaatgcagcc ataatgacga
     7801 tcccggttac tgtaccgaac attgtgaacg gtgcatgcaa tttaagctct tgcgcaatca
     7861 tgcccgcttt catagggcaa ctcctgaaga gaaaccagat atgacaaaac agtttgtggc
     7921 cgcgttcaat ccaataaaaa atcctgcatg acggcttttg gcaaaatatt cggtctgacg
     7981 aacaaaacag gttatgcctt ccgacggcat tttcatgaac ggtaccctcg ttttcccctt
     8041 tttgctgctg tggattcatc cacctcatcc tgccagccgt ttttccggat atcatgcaga
     8101 tcgaattttg ccgtataggg cttgatgtcc aaaagcggag ttccgtcgag aatgtcaatt
     8161 ccatccacat aaaggatatt gtcctctata cggatgagtt ctacaacgct aagcccaaga
     8221 ggattgggac gacccggcgc gcgtgtggaa aatattcccc gctccacgtc ctgcagaaac
     8281 ggtttgactt tcagttgaac aagcggcgcc tgatgaaaat gatagatcag gtaaacgtgg
     8341 gagaagccgt ccagatcgct taatccagat atatattttt caaacacttc aatgcgtccc
     8401 ttgcaaccgc aagcgtaaac cggctgaatc ggtgtttttt cggcaaccgt gtgttcactg
     8461 tgaatgattc caatcggatg atatatgatt tgtcggttta tcatacccag tgtccatcca
     8521 catcggccga tttggcctcg gttaacgtgc cttcacgata ttgttccaga gcctcgctga
     8581 ccgtagcggc ggacgtattg tatatcctga tgcctgccgc ctgaagcgtc cggaaggctt
     8641 tgggcccgca gtgtcctgta atcagcgctt ttacgccaag gcgggcgatg ttttgtgccg
     8701 attgaatgcc ggctccctgc gccgcgttaa gattctgttc attatcaaca atttcgaagg
     8761 tattcgcagc ctgatcatag attagaaatt tcggcgcccg tccgaatctg ctgtccagag
     8821 gagcattcaa gtctacgccc gatgttgtca atgcaatttt cattaaaaaa attctccttt
     8881 gataactttt tcatagagag cagtgacggc gctaagcggt gacttgcgcg ccatcactgc
     8941 aatgcataga taagctctat gcgggcttcg caaaagaggc taattatttt gcgtatctgt
     9001 ctttagctcg tccaaccgtt ttttaatggc gtctaattcc ctctgcagaa attcagcctg
     9061 cgttctgaga gcctgttgtt cctcatgggg atcaggaggg acaaccgcgg cggagtattc
     9121 atcggttcgt cgccatcccg gtattgcagc ggcataaaaa cggttgccaa agccgaatcc
     9181 accgccacgg ccacctcggc cgccaaaacc ggcgccacgt ccgaaacctg ctccaaaacg
     9241 tcttgccgga atatgattca tataaccggg cgctccgaaa cctgcgcaat atccggctcc
     9301 tctacctgtc atcggtccca ttccaatggg gcctgttgca tctcctcttg gcataatgaa
     9361 gacctccttt ttttagttat tcccgaatca tggttgaacc gcactgcggg cacttgactt
     9421 gcgtgcaggg aattccccta tcatgcggtt gacggttacc gcatttcggg catagacagt
     9481 tgcccaaagc tccgcccgcc ttcatccctc ccatgcgtcc tcggccaccg ccgccgcgtc
     9541 ctcggcccat accggaaccg gaaccttggc tcataccgcc gcccgttcct cttggatttc
     9601 tattatttgt attcatgatg ttttctccct tttggcgtct cttttttaga aaacgaaaac
     9661 tgccgccgcc agtaccgtgg tatagtcttt tgttttcaaa accgtcgatt gcgttgtatt
     9721 tctcgttccg acgattttac cgctgatttt aaacagttcc tttttctcgt cccagctttc
     9781 atcaatgttg aaatcaatcc ccagcgtgga tgccagcatc gctgcggcca aatcttccgc
     9841 atagtctccg gtctgttttt cgttataacc gaacgaatga tgctcgctga tataaccgta
     9901 cgttttctta tccatgggaa tcgcacagcc gacggacgcc gaaatcaaac ggcgcggctc
     9961 gttgctgcag cagcggctca taacgcagaa ggtgatggcg ccgggtctga gctttttcag
    10021 tccttcggtg cgtgaaataa tgcggcagcc cggcggaaga atgctcgaaa cctgaacaag
    10081 gttgcatttt tcaatgcccg cgtcacgcag cgccagctca aaagaatgaa gctgttcgcg
    10141 atgcgtgccc acgcctttgg taaagaatat ttctttgggg atgtactggt tcactgtctt
    10201 gtctcctttt ctgcaatgat tattttcggg tctttatctg ctgcggtaac gcataccgcg
    10261 ctgtttgcaa aatcctgcgc ccatcgctct tctccggcag ccgggcaaag agaacacagg
    10321 caatcccagc gtgttgttcc ggtaggcggt tataatggca tggacctctc ccctgacaaa
    10381 gggatgaacg atgattccct gggccataat caacgcctgc ataggacggg aaatggcgcc
    10441 gcaaattaga acatcgacgc cccgattttt tagttcagcc gcacgcccgg caggatccgt
    10501 cgcgacgaat ttctgcaatg ttcttttatc aggattcgcc gtgtctgctt caacaattaa
    10561 aagttgctcc gcggaatcga aaaccgtgga aatgcaatcc ttaaataccg acagtgccag
    10621 cttcattaat gctccttagg atgaaatacc tatagaacat tcatagcaaa atcaatgcca
    10681 gaaggtgttt ttaaaattta gtgtttgatt acaggttatt ggaaatgacg gttattcgct
    10741 tccatgaaaa ataagtagcg ttaatgcaac gatagttgca aaaagtcgca ggaatgcgac
    10801 tacagaggga attgccccgg aagggtaatg ccgagtgctt tgatttttct gtaaagggtg
    10861 cttttatgaa tgcccagttg ccttgccgtt tcccggcaat tccagttgtt tttttgaaga
    10921 gcgttgatga gccaggccgc ttccacgtca cgaagagagg acgcatcggt tctgattttc
    10981 tggggcggag acgcaaaaag agattccggt agatgctttt tttcaatccg gccttctttg
    11041 cagaagatga aggcgcgttc gataatgttg gccagttccc ggatattgcc cgggtaagtg
    11101 taagccatca gaacggacag tgcttcctga ctgacgcctt caatctcttt tttttgcaga
    11161 agatcaaagc ggctgataaa gtgatcaatc aacagtggaa tatcttccat gcgttccctc
    11221 agcgggggaa gggtgatttt gaagacattg atgcggtaaa acaaatcttc ccggaaacgg
    11281 ccctgtctga ccagatcctc gagattttta ttggttgccg cgatcacacg gacattgtgc
    11341 tcaacgcttt gtgttgcgcc cagcggttca taggtttttt cctgcaggac gcgcaggagc
    11401 ttaacttgca tggcgggggt gatttctccg atttcgtcga gcaataacgt tccgttttct
    11461 gccagtttaa aacggccggg cttgtctttt ttcgcgtcgg taaacgcccc cgctttatag
    11521 ccgaacagtt ccgactccag aagtgtatcc ggcagggcgc tgcaatttac cgcgacaaag
    11581 gacgttttgc ggcgcggact caggttgtgg atggcccggg cgatcagctc tttgccggtt
    11641 ccgctttcgc cttcgatcag caccgtactg ccgcctgccg caatatcggg caggatatcg
    11701 aacaactgca gcattttatg attcttggaa agaatatcgg caaagttgta tttctgtaaa
    11761 accgccttgc gtaaatcttc ttcgacgctg atgtcccgga atgtttccac cgctcccagc
    11821 aattttccct tttgatcgcg caggagcgcc gtggaaatac tgacgggaag tttgtgccct
    11881 tccgcgttga tgatggctac ttttttattg atgatctgct cgcccgtttt cattgtcttg
    11941 cgcaggcagc agtctttttc gcaaatatcc gcttttaaaa catccttgca gcgttggccg
    12001 atggcttcct cccggggaac cccggttatt ttttcagcgg cacggttgaa agacgtgatc
    12061 agccagtcct ggtctaccgt gaaaacgccg tcggcgatcg aatccagaat aatatcctgt
    12121 ttggttccga tatccggcga tccctgtttt aaaggcatct tttttttcat gtctgttttc
    12181 cgatgacggg tgtcggattt agtgacagcc gtgttcgtgc tgggtgcagg aaattcccga
    12241 atctttcagt gtgccggcaa gccagctttc cgtaactgtc ttcacgtttc cggaacagcc
    12301 gcgcagaaca tcaatgccat ggctgttcaa cacattgacg gcgccctgtc ccatattgcc
    12361 ggccagcatc agcttgacgc cctttgcagc cagcacttcg gcgatgttgg atttacaccc
    12421 gcagccggcg ggtgaagaaa ccttttcctc ctcgacaatt ttcttttcct ggtcaatggt
    12481 gaaaaccgta aaatactcac agtgtccaaa gtgttcatca atctgattct gataagaggg
    12541 taaagcaatt ttcatacata tctccttgca aagcattttt catttattcc tgcgttttga
    12601 atgttcgcgg gttttctatt catcaaaatc gaagtcgccg ccgattctta atgcttttcc
    12661 gttgatcagc acatcggcga tctttttgtg tgcgacgtct atgattctgc cgaaggtttg
    12721 acgtgaaatg ttcatctttt cggcggcttg tttctgatac aaatgttcgt agtcggcaag
    12781 acgaaccgcc tcgaattcgt ccatattcag aacaatttct tcgagctcca tggagggtat
    12841 tcccctgggc ttgaagtatt tcttgtcggg cgcacccatg atgtgacggt ggcatttcgg
    12901 ccggggcatt gtttgtatga actccttttg ttagaatata agcatatgct caaaacttgt
    12961 caagggttaa aatttcctcg taaccgtatt taaagtcgtt aacgcgagtt ttagaaaaga
    13021 taatcgttgg aggaattcct actgcccgat accatttcgc ttcttccggt gaccgtaaga
    13081 ggacatttga ttgttgtgaa aaaaaaacgg ctgtggtaac ttcttgcgca attcgat
//
LOCUS       DAOOLB010000058        12886 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_5341_length_12886_cov_37.466371, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000058 DAOOLB010000000
VERSION     DAOOLB010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 12886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12886
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5341_length_12886_cov_37.466371"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..711
                     /locus_tag="PLF77_07840"
     CDS             <1..711
                     /locus_tag="PLF77_07840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08803.1"
                     /translation="ESLEEKVQERTRELEAFSYSVSHDLRAPLRTIEGFSLALVEDYS
                     DKLDNQAKDYISRIRRATEIMSELIDDMLKLSRITRTEIDKADINLSNIALTVVDELR
                     KAHPDRPVKVTIPDSLTDYADSRLIKIVLENLLGNAWKFTGKKTDAEIEFGSTQQNGK
                     KVYFVRDNGAGFDMEYVDKIFAPFQRLHNIEEFPGTGIGLATVRRIISRHGGTVWAES
                     VPHQGATFYFTLGYPPPA"
     gene            789..2546
                     /locus_tag="PLF77_07845"
     CDS             789..2546
                     /locus_tag="PLF77_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC08804.1"
                     /translation="MNEVLRVLIVDDSDDDTNLVLRKLRSGGYQPEWERVDTHDAMKN
                     ALEKKTWDVILCDYKMPGFSAPAALKLAQEINPNIPFIVVSGAIGEETAVEVMKAGAH
                     DYVMKDKLAKLSVAVKREIAEAKIRRDKKSMEDMLKRSENNFRNSLDESPLGVRIVTS
                     DGELLYANREILNMFGYDSIDELKSISHLQRYTPETFVEYEKRIEMRKHGEYVPPNYE
                     VSIVRKDGTIRHLEVFRKQVLWNGQMQFQALYNDITERKQAEKNLRESEKRYRVVVEN
                     ANEAILITQNMKILFANTVAINNTGYPMEELTSMNFSDLIHPDDIGMVRGYHDRRIKG
                     DAIPSVYSFRIVCRDKSVKWVELNTTVIEWEKKRAALNFLKDITVSKQLEEERSQSFI
                     KIKKTLDATVSAIASMVETRDPYTTGHQKRVSILARAIAEEMGLCMDVVEFIETAAII
                     HDIGKLSVPSEILSKPTKLTNLEFELIKTHSQSGYNILKDIEFPWPVADAILQHHERM
                     NGTGYPQKLQGHDILLESRILAVADVVEAISSHRPYRPGLGIDFALEEITKNKGVLYD
                     ENVVDACIKLFREKHFVLS"
     gene            2724..3986
                     /gene="hisS"
                     /locus_tag="PLF77_07850"
     CDS             2724..3986
                     /gene="hisS"
                     /locus_tag="PLF77_07850"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HPC08805.1"
                     /translation="MEKITAIRGFKDILPDDALAFCRIESLARTIFDSFGFREIRVPI
                     LEKTELFKRSIGETTDIVEKEMYTFTDRDNDQITLRPEATASVIRAYIEHNMSANESV
                     TKLFTFGPMFRRERPQKGRFRQFNQIDVEVFGDDKPQTDADVIFMLMHFLNSAGLRNL
                     QLEINSLGCSACRPEFSKAVIDFLKDRQSRLCPDCQRRIHTNPLRVFDCKVPSCAETL
                     AGAPQIIDFLCSDCTQHFAQVQSFLADLQTPFVINARMVRGLDYYTRTAFEVTTGILG
                     AQNAVAGGGRYNGLVSFLGGPDVTGIGFAVGFERLVACLPENRKDTYTTDLFIAALGA
                     NAQHIAFGLTNRLRVCGLSAEMDYSDKSLKSQMKRADKLNSAYALIFGDKEIEEKQVV
                     LRNMQTKQQQDIPMDGLHESIIKIIKER"
     gene            3993..5762
                     /gene="aspS"
                     /locus_tag="PLF77_07855"
     CDS             3993..5762
                     /gene="aspS"
                     /locus_tag="PLF77_07855"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPC08806.1"
                     /translation="MAEFLTTDKRTHYCGNLDASLIGQDVVLMGWTHRRRDHGGVIFV
                     DLRDREGMVQVVFNPDNAKAHKEAHRIRSEFVLAVKGKVRRRPDGMENPSLKSGEVEV
                     LVSDLDIMNESKTPPFSFDDEEISENIRLKYRYLDLRRPAIQKNLFLRSRMAAAARRY
                     FEENGFIEVETPFLGKSTPEGARDYLVPSRIYKGMFYALPQSPQIFKQLLMISGFDRY
                     YQIVKCFRDEDLRADRQPEFTQIDVEMSFITEDDIMQMMEGLMKAIFKSCLDIDLALP
                     LPRLTYAAAVSRYGKDNPDVRFGMEIVDLTSIVKDSGFKLFAEVASSGGVVKAIKAKQ
                     ASVLSRKDLDGLKDVVAVYGANGLAWAKINAADWTSPVFKYLKPDEVQAIGNALDARE
                     GDIIFFIADKPKVASDALGNLRLHLGKKLGLIDPKALAFTWITEFPLMEYSETEKRFV
                     STHHPFTSPFLEDLPLLTADPGKVRARAYDLVLNGSEIGGGSIRIHHKDVQSQVFSAL
                     KLSDEDARLKFGFLLDALEYGAPPHGGLAFGLDRLTMIMTGAESIRDVIAFPKTQKAA
                     CLMTDAPSKVSIEQLMELSIKIV"
     gene            5863..7755
                     /locus_tag="PLF77_07860"
     CDS             5863..7755
                     /locus_tag="PLF77_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone-lysine N-methyltransferase"
                     /protein_id="HPC08807.1"
                     /translation="MAKIKKENKPTAAQKSKIKKAALVDVDQPNLYKEIFPYSEVSKI
                     VFDHQTVPMEPAQEIFITDTTFRDGQQSRPPYTAEQIASIFQLLSRLGGPNGVIRQSE
                     FFLYSKKDKDAVEKCMALNLRYPEITSWIRASKDDVPLVKQFGLKETGILTSVSDYHI
                     FLKLNKKRSQAMNDYLGIVKTILDLGIIPRCHFEDVTRADIYGFCIPFAQQLMKLREE
                     SGIDIKIRLCDTMGYGITYPGAALPRSVDKLVHAFIHDAGVPGHLLEWHGHNDFHKAM
                     INATTAWLFGCSAANSTLLGLGERTGNPPIEGLIIEYIALKGRNGIDTTVITDIADYF
                     EKEIGVKIPHNYPFVGSGFNVTSAGVHADGLIKCEEIYNIFDTRKILKRPITTTITDK
                     SGNAGIAFWINQRFNLEGNKTVDKRHPGVSKIHKWVMEQYESGRITSISPEEMEKKVR
                     LYLPERFMSELEKIKYIAEKAAVAAVTHIIEQPLMKTMDTAKQEPLMQKFVDEYPSVQ
                     FAYVVDMNGRKTTRNITDIADRAKYENYGVGTDQSDRQWFINPLRTGEVYVSDFFISK
                     MTGVLCFTVSAPIFNTDDEMVGIFGVDIKFEDWVKRAEDMEDMDHIALHEEYKEMKSK
                     AKHGHH"
     gene            complement(7875..9323)
                     /locus_tag="PLF77_07865"
     CDS             complement(7875..9323)
                     /locus_tag="PLF77_07865"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HPC08808.1"
                     /translation="MKTGLIVYMIALLVFYLGVSMFLPLFVALVYQDGSAFAFFVSIL
                     IACCLGGVVYLFTRGQKDFHLTHRDGLAIVTFGWIAAGFIGTLPYLLSGSIPDFTMAY
                     FESVSGFTTTGASILKDVQHLPPAVLFWRAQTQWMGGMGIIVLSIAILPFLGIGGMEL
                     YKAETPSPVVDKLTPRISSTAKLLWKIYIFLTLFLIILLLLGGMPLYDSICHSFAAMP
                     TGGFSTKNVSVGYYQSSYIDYVILLFMFIAGVNFSLHYKLVSGHPKQFFRDPEFRVYL
                     YITVIAILVVSANIYGTVYPRIQDALRYASFQVVSIMTTTGFVTADFEQWPVFSQLIL
                     FVFMFLGGMAGSTAGGIKIMRIILLVKHCYLEIFRIIHPHATTMVKLGRTPVSQTIMR
                     SIWGFFILYLVIFMIGAIALAALGLDIISALTAMATCLGNVGPGLGSVGPMDNYSVIP
                     VTGQWILIVSMLLGRLEIYTIIVLLMPAFWRK"
     gene            complement(9323..10681)
                     /gene="trkA"
                     /locus_tag="PLF77_07870"
     CDS             complement(9323..10681)
                     /gene="trkA"
                     /locus_tag="PLF77_07870"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPC08809.1"
                     /translation="MRVIIVGAGEVGYHIAKKLSDEKRDVVLIDKDPGKIRRINENLD
                     VQAFLGSGTSPKMLRQAGIQDAQLLVAATDMDEVNLIACLLARNLNRYAVKIARVRNQ
                     EYLQEKSLFSKDLLGVDLIINTQSMMVETILNLMDIPGASEVIDFLGGAVKLVSFLIF
                     EDSPLAGKKLSELSNQEGKLLVGAIVRGHKILIPNGNDTIQPMDLVYFVIKKSELDQT
                     LSRLHMPAKPMRNVIIVGAGGTGMAVAKALDQRRLNVKLIDQDGAKCKEANRLLEKVV
                     VIKGDGGDRDLLIEENARAMDMLIAVTDDEESNVLISLLAKELGTARTITRVSKLSYM
                     HVVSNVGLDTVINPRMAAVRAILQYIRRGKIISVAPLREEQAEAIEAEALETSDIVNV
                     PLSKVDFPPGAIIGAILRGDAIVIPTGSTVVLPHDHLIIFAVQKVINKVEKLLTVKLE
                     YF"
     gene            complement(10866..12836)
                     /locus_tag="PLF77_07875"
     CDS             complement(10866..12836)
                     /locus_tag="PLF77_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPC08810.1"
                     /translation="MIDYAKELNPEQYRVVMEEGGPLLVLAGAGSGKTRTLTYRVARL
                     LESGVAQENILLATFTNKASHAMLNRVESLIHAETRNIMGGTFHHVAHLILRRHAHYL
                     GYKTNFSIVDSEDARQIISTCMAELKIDPKLSKFPKSNIVGDIISLSVNTETTVENVL
                     MSRYSFFLHLMDEIIQVASLYEQKKRELAVMDFDDLLSNCRMLLQENADVLKNLSGRF
                     QYVLVDEYQDTNLIQADLIDLLASTHKNLMVVGDDSQSIYSFRGADFNNIINFPHRYP
                     DCKIFKLETNYRSTPEILHLANLSITNNEKQFPKELKAVRKNGMRPVIVSAQNVVKQA
                     DFVAQRVTELMRGGVPLREIAVLYRAHYHSMEMQMEFVRRDIPFDMRSGIRFFEQAHI
                     KDVTSYMRVLVNPRDELAWRRLLIMYPKVGKATFNKIWHYLKKEDNPLNAVLSEDFIK
                     AMSKSAKPGLEECRKTIRQLLDLPENERIPERIIDVLLNRGGYRVYLQDNYSDASSRE
                     EDLIQLGNFSAQFLQMEDFLNELALLTNMAKEENIGEEDDEDKVALSTIHQAKGLEWS
                     YVFLIWCADGMIPLQKALKEQDGEEEERRLFYVAVTRAKDQLYLCYPALDYTRSSGVL
                     NMAPSRFIREIAPLSEYDADRPFEQWMLCDDY"
BASE COUNT         3464 a         3262 c         3115 g         3045 t
ORIGIN      
        1 gaatccctgg aagaaaaggt tcaggagcgc accagagagc tggaggcgtt ttcttattcc
       61 gtatcgcatg acctgcgggc gccgttaaga acaatcgagg gattcagcct tgcgctggtt
      121 gaggactaca gcgataagct ggataatcag gccaaagatt atatcagccg aatcaggaga
      181 gcgaccgaaa tcatgagtga gctgatcgac gacatgctga aactctctag aataacgcgc
      241 acggaaattg ataaagccga tatcaacctc tccaatattg cgttgacggt cgttgatgaa
      301 cttcgcaaag cccatcccga ccgtcccgta aaggtgacca taccggattc cctcaccgat
      361 tacgccgatt cgagattaat aaaaattgta cttgaaaatc tcctgggcaa cgcctggaaa
      421 tttacgggca agaagacgga cgctgaaatt gaattcggct caacgcagca gaacgggaaa
      481 aaagtatatt tcgttcggga taacggcgcc ggtttcgaca tggagtatgt ggataaaatt
      541 ttcgctccgt tccaacgctt gcacaacatc gaagaatttc ccggaacggg aatcgggctg
      601 gccacggtca ggcgaatcat cagccgtcac ggaggaaccg tctgggcgga aagcgttccc
      661 catcaaggcg ccacttttta ttttacgctg ggctatcccc cgcctgcata aaacacattt
      721 ggaagaaggc cgttggatga cggaagacaa aagatgattg cattgcataa attaagggag
      781 aaacgaatat gaatgaagtg cttcgcgtcc tgattgtgga tgattccgat gacgatacca
      841 atctggtttt acggaaactg cgcagcggag gttatcagcc tgaatgggaa cgagtggata
      901 cgcatgacgc tatgaaaaac gctctggaga aaaaaacatg ggacgtgatc ctgtgcgatt
      961 acaagatgcc cggcttcagt gcgccggccg ccctgaaact ggctcaggaa atcaatccga
     1021 atattccctt tattgtcgtt tccggcgcca tcggcgagga aacggccgtc gaggtcatga
     1081 aggccggcgc tcacgactat gtcatgaaag acaagctcgc caagctttct gtcgccgtga
     1141 aacgggaaat cgccgaagca aaaatcaggc gggataaaaa aagcatggaa gacatgctca
     1201 aaagaagcga aaataatttc cgcaattcgc tcgatgaatc tccgctgggc gtacgtattg
     1261 tcacgtccga cggtgagtta ttgtatgcca accgggaaat tttaaacatg ttcggctacg
     1321 atagcatcga tgagttgaaa tccatttccc atctgcagag atatacgccg gaaacctttg
     1381 tcgaatacga aaaacggatt gaaatgagaa aacacggcga atatgttccg ccgaattatg
     1441 aagtcagcat tgtccgcaag gacgggacca ttcgccacct ggaggttttc cgcaagcagg
     1501 ttttgtggaa cggccaaatg caatttcaag ccctttacaa cgatatcacc gaacgcaagc
     1561 aggccgaaaa aaacctgcgg gaaagcgaga agcgctatcg cgtcgttgtg gaaaacgcca
     1621 atgaagccat tttgatcacg cagaacatga aaatcctttt tgccaatacc gtggccataa
     1681 acaatacagg ctatcccatg gaagaactga cctccatgaa tttcagcgat cttatccatc
     1741 cggacgatat cggcatggtc aggggttatc acgacagaag aatcaaaggc gatgccatac
     1801 cgtctgttta ctctttcagg atcgtttgcc gcgacaaatc cgtgaaatgg gtggagctaa
     1861 acaccaccgt aatcgaatgg gagaaaaagc gtgccgcgct gaatttttta aaggacataa
     1921 cggtcagcaa acaactcgaa gaggaacgct cccaaagttt tataaaaatc aagaaaacgc
     1981 tcgatgcaac cgtaagcgct attgcctcca tggtggaaac cagggatccg tatacaacgg
     2041 gtcatcaaaa acgggtgtcg attctggccc gggccattgc tgaggaaatg ggattatgca
     2101 tggacgttgt ggaattcatc gagacggccg caattattca cgacatcggg aaattatccg
     2161 tgccttcgga gatcctcagt aaaccgacca agctcaccaa tcttgagttt gaactgataa
     2221 aaacacattc ccaatccggt tacaacattc tgaaggatat tgaatttccc tggcctgttg
     2281 ccgatgccat tttgcagcac catgaaagaa tgaatggaac cggttatccc caaaaactgc
     2341 aaggccatga tattcttttg gaatcgcgaa tactggccgt tgccgacgtc gtcgaagcca
     2401 tttcgtccca caggccttac cggcccggcc tgggcattga ttttgctctg gaagaaatca
     2461 ccaaaaacaa aggcgtgctt tatgacgaaa acgttgtgga tgcctgcata aagctcttcc
     2521 gcgaaaagca ctttgttttg agttaagaag cgcctcccgt cacacaaatg tatgcgtcca
     2581 ggatgcaatc gtttgtgcgg tgcgaaagcg ttgatttctt tctcattgca tccattgccc
     2641 cttcccctgc ttcaacctat tttctcgttg ccaagaagac atgaatgtgt tagtaatgca
     2701 cccttaaaaa tttggttggg attatggaaa aaattacagc aattcgaggt tttaaagata
     2761 tcctgccgga cgatgcgctt gctttctgca gaatcgaatc cctggcccga acaatttttg
     2821 attctttcgg tttccgggaa atacgcgtcc ccattctgga aaaaacagaa cttttcaagc
     2881 gaagcatcgg cgagaccacc gatattgtcg aaaaggaaat gtacaccttc accgaccggg
     2941 acaatgacca gatcaccctg cgaccggaag ccaccgcgtc ggtcatccgg gcttacatcg
     3001 aacacaacat gtccgcaaac gaatccgtta caaagttatt taccttcggt ccgatgttcc
     3061 gccgcgaaag gccgcaaaaa ggtcgattcc gccagtttaa tcaaatcgat gtggaagttt
     3121 tcggcgatga caaaccccaa accgacgcgg acgtgatttt catgttgatg catttcctga
     3181 acagcgcggg tttgcgcaat ctgcaactgg aaatcaattc cctgggctgc agcgcctgcc
     3241 gtcccgaatt ttccaaggcg gtgatcgatt tcctgaagga caggcaaagc cgtctgtgcc
     3301 cggattgtca gcggcgcatt cacaccaatc ccctgcgggt cttcgactgc aaggttccct
     3361 cctgcgccga aacccttgcc ggtgcgcctc agatcattga tttcttgtgt tcggactgca
     3421 cgcagcattt cgcacaggtt caatcgtttc ttgccgattt gcagacaccg tttgtcatca
     3481 acgccagaat ggtccgcggg ctggattact acacccggac ggcctttgaa gtcaccaccg
     3541 gcatcctggg cgcccagaac gccgttgccg gcggagggcg ttacaacggc ctggtcagct
     3601 ttctgggggg gccggacgta accggaatag gctttgccgt cggttttgag cgtctggtcg
     3661 cctgcctgcc ggaaaaccgg aaggatacct atacaaccga tcttttcatt gcggcgctgg
     3721 gcgcgaatgc acagcatatc gccttcggtt tgaccaaccg gctcagagtt tgcggcttat
     3781 ccgcggagat ggattactcg gacaagagtc tcaagtccca gatgaaacga gcggataagc
     3841 tcaacagcgc ctatgctctc attttcggcg acaaggaaat tgaagaaaaa caggttgtct
     3901 tgcgcaacat gcagacaaaa caacagcagg atattcccat ggatggcctg catgaatcca
     3961 taataaaaat catcaaggag aggtaaaaag ttttggctga atttttaacg actgataaac
     4021 ggacacacta ctgcggaaat cttgatgctt cactgatcgg ccaggacgtc gttttgatgg
     4081 gctggacgca tcgccggcgc gatcacggcg gcgttatttt cgtcgattta cgggatcgcg
     4141 aaggcatggt gcaggtggtt tttaatcccg ataacgcaaa agcccataag gaagcccatc
     4201 gaatccgttc tgaatttgtt ctggccgtca aaggtaaggt gcgcaggcga ccggacggca
     4261 tggaaaatcc gtccctgaaa agcggggaag tcgaagtcct cgtctccgat ttggacatca
     4321 tgaacgagtc caaaacgccg cccttctcct tcgatgatga agaaatttct gaaaatatcc
     4381 gtttgaaata ccgttatctg gatttgcgcc gtccggcgat acaaaaaaat ctttttctgc
     4441 gcagcagaat ggcggcggcg gcgcggcgct atttcgaaga aaacggtttt attgaagtgg
     4501 agactccgtt tctgggaaaa agcactccgg aaggagcgcg cgattatctg gtgccgagcc
     4561 gaatttacaa gggcatgttt tacgcgctgc cgcagtcgcc gcaaatcttc aagcagttgc
     4621 tgatgatttc cggtttcgac cgttattacc agatcgtcaa atgtttccgg gatgaggatt
     4681 tgcgcgccga ccggcagccg gaattcacgc agattgacgt ggaaatgtct ttcattaccg
     4741 aagacgacat catgcagatg atggaagggc tgatgaaagc tattttcaag agctgcctgg
     4801 atatcgacct cgcccttccc ctgccccgtc tgacgtatgc cgctgccgtc agccgctacg
     4861 gaaaagacaa tcccgacgtg cggttcggca tggaaattgt ggatttgacg tccatcgtca
     4921 aagattccgg ctttaagctt tttgcggaag tcgcttcttc cggcggcgtc gtcaaagcca
     4981 tcaaagcgaa acaggcctcc gtactttccc ggaaagacct tgacggcttg aaagatgttg
     5041 tggccgttta cggcgccaac ggtttggcct gggccaagat caacgccgcc gactggacat
     5101 cgcctgtttt taaatatctt aagcccgacg aagtgcaggc catcggtaat gccctggacg
     5161 ccagggaggg cgatattatc ttcttcatcg cagacaaacc caaagttgct tccgatgccc
     5221 tggggaattt gcggcttcat ctgggcaaaa aactgggact gattgatccc aaagcgctgg
     5281 ccttcacctg gattacggaa tttcccctga tggaatattc ggaaacggaa aaacgttttg
     5341 tctccacaca tcatccgttc acgtcgccct ttctggagga tctgccgctt ttaaccgccg
     5401 atccggggaa ggttcgcgcc agggcttacg accttgtgct caacggttcg gaaatcggcg
     5461 ggggcagcat tcgtatccac cacaaagatg ttcaatctca ggtcttcagc gcactgaaac
     5521 tcagcgacga ggacgcccgg ctgaaattcg gtttcctgct ggatgccctc gaatacggag
     5581 caccgcctca cggcggtctg gcgttcggtc tggacaggct gaccatgatc atgaccgggg
     5641 cggaatcgat ccgcgatgtc attgccttcc cgaaaaccca gaaagccgcc tgtttgatga
     5701 ccgatgcgcc gtcaaaagtc agcattgaac aattaatgga gttatcgata aaaatcgttt
     5761 aagcaaaatg cccatacgca gaatcagcgc gtctgtaccg ttcgtatgag ccgcgctgac
     5821 ttacaaaacc aggaatgaac acattcaata ccggagatat acatggctaa aataaagaag
     5881 gagaacaaac cgacggctgc gcaaaaaagt aaaatcaaaa aagcggcatt ggtcgatgtg
     5941 gaccaaccca atttgtacaa agagatattc ccttattcgg aagtcagtaa aattgttttc
     6001 gaccatcaaa ccgttcccat ggaaccggcg caggaaattt tcattacgga tacgaccttc
     6061 cgcgacgggc agcagtcccg tcctccctat accgccgaac aaattgcctc catattccag
     6121 ttattgagca ggctgggcgg gcccaacggg gtcatccgtc aatcggaatt ttttctctac
     6181 agcaagaagg acaaagatgc cgtggaaaaa tgcatggccc tcaatctgcg ttatccggaa
     6241 atcaccagct ggatcagggc atccaaggac gatgttcccc tggtcaagca gttcggcctg
     6301 aaggaaacgg gcattctgac ttccgtctcg gattaccaca tctttctgaa actcaacaaa
     6361 aaacgaagcc aggccatgaa tgattatctg ggaattgtca aaaccattct ggaccttggc
     6421 attattcccc gttgccactt tgaagatgtc acccgcgccg acatttacgg tttttgcatt
     6481 ccttttgccc agcagttgat gaagcttcgg gaggaaagcg gcatcgacat caaaatcagg
     6541 ctctgcgaca cgatgggtta cggtatcacc tatcccggcg ccgcccttcc ccgcagcgtc
     6601 gacaagcttg tccatgcatt tattcatgac gccggcgttc ccggccatct gctggaatgg
     6661 cacggacaca acgactttca caaggccatg atcaatgcaa ccaccgcctg gcttttcggt
     6721 tgcagcgccg ccaattccac gctgctgggt cttggcgaaa gaaccggtaa tcccccgatt
     6781 gaagggttaa ttatagaata tatcgccctg aaaggaagaa acggtatcga caccaccgtc
     6841 atcaccgata ttgcggacta tttcgaaaag gaaatcggcg tgaagatacc ccataattat
     6901 ccgtttgtcg ggtcgggctt caatgtcacc agcgccggtg tccatgccga cggtctcatc
     6961 aaatgcgaag aaatttacaa tatctttgat acgcgaaaaa ttttaaaacg accgatcacc
     7021 acgaccatca cggacaaatc cggcaacgcc ggtattgcgt tctggatcaa ccagcgtttt
     7081 aatctggaag gcaataaaac ggtggacaag aggcatcccg gtgtttccaa aattcacaag
     7141 tgggttatgg aacagtatga atccggacgc attacttcca tatcgcctga agaaatggaa
     7201 aagaaagtcc gtctttacct gccggaacgc ttcatgtccg agctggaaaa gatcaaatac
     7261 attgcagaaa aagccgctgt tgccgccgtt acgcacatca ttgaacaacc cttgatgaaa
     7321 accatggata cggcaaaaca ggaacctctg atgcagaaat tcgtcgacga atatccttcc
     7381 gttcaatttg cctacgttgt ggatatgaac ggccggaaaa cgacacgaaa tattaccgat
     7441 atcgccgatc gcgccaaata tgaaaattac ggcgtgggaa ccgaccagtc cgacaggcag
     7501 tggtttatca atcccctgcg caccggcgag gtctacgtct ccgatttctt catttccaaa
     7561 atgaccggtg ttttatgctt cacggtttcc gcccctattt ttaatactga cgatgagatg
     7621 gtgggtattt tcggtgtgga tattaaattc gaagactggg tcaagagagc ggaagatatg
     7681 gaggacatgg atcacatcgc gctgcatgaa gaatacaaag aaatgaaatc caaagccaag
     7741 catggccacc attgatcata accgcgtctt gtaaagacgt gaaacattgc tccggcgcgg
     7801 ctccgtcaga tagagatgtt tatctttcaa acaaaacgca acggcctcgc gaaccgaacg
     7861 ctttcagccg ggcattattt gcgccagaag gcaggcatca gcagaacaat aatggtgtaa
     7921 atttccagac gtcccagcag catcgacaca atcagaatcc actggcctgt cacgggaata
     7981 accgaataat tgtccatggg gccgacactg cccaaccccg gcccgacgtt acctaaacag
     8041 gttgccatag cggtcagcgc tgaaatgata tccaggccga gagccgccag tgcaatagct
     8101 ccgatcatga aaatgaccag atagagaata aagaagcccc aaatgcttct catgattgtc
     8161 tgggaaaccg gcgtcctgcc gagtttgacc atcgtcgtgg catgaggatg aataattctg
     8221 aatatttcca gataacaatg cttgacaagc aggatgatcc tcatgatctt gataccgccc
     8281 gcggttgacc cggccatccc tcccagaaac ataaagacaa agagtatcaa ttgcgaaaag
     8341 acaggccatt gttcaaagtc tgcggttaca aatccggtgg ttgtcatgat ggaaacaacc
     8401 tggaatgatg catagcgtaa agcatcctgt atccggggat aaaccgtccc gtaaatattg
     8461 gcagacacga cgagaatggc aatcacggtg atgtaaaggt aaacgcgaaa ttccggatcc
     8521 cggaaaaatt gtttcggatg cccgctgacc agtttataat gcagcgagaa gttcacgcct
     8581 gcaatgaaca tgaacaggag aatgacataa tcgatataac tgctctggta atatccgaca
     8641 ctgacgtttt tcgtcgaaaa tccacccgtc ggcatcgcag caaaactgtg gcagatggaa
     8701 tcataaagcg gcataccgcc cagcaacagt aaaataatca gaaaaagcgt gagaaaaatg
     8761 taaattttcc agagcaattt ggctgtgctg gaaatccgcg gcgttaattt gtccacaacg
     8821 ggactcggcg tctcagcctt atagagttcc atgccgccga tccccaggaa cggaagaatg
     8881 gcgatggata acacgataat ccccatgccg cccatccact gcgtttgagc gcgccagaaa
     8941 agtactgcgg gcggcaaatg ttgcacgtcc ttgagaatgc tcgccccggt cgttgtaaac
     9001 ccggagacgg attcgaagta agccattgta aagtccggaa tcgatcccga aaggagatac
     9061 ggcagcgtcc cgataaaccc cgcggcgatc catccaaagg tgacaatggc caagccgtcc
     9121 cgatgcgtca aatgaaaatc tttttgaccc cttgtaaaaa gatagacaac gcctcccagg
     9181 caacatgcaa tcaggatgga aacaaaaaaa gcgaaggcgc tgccgtcttg atacacaagc
     9241 gctacaaaca gcggcagaaa catggaaacg ccgagataga ataccagcag tgcaatcatg
     9301 tagacgatta aaccggtttt catcaaaagt actccagctt gacggtaaga agtttctcca
     9361 ctttgttgat gactttttgc acggcaaaga taatcaggtg gtcatgagga agcaccacgg
     9421 tactgccggt ggggataaca attgcatccc cgcgtaaaat agcgccgatg attgcgccgg
     9481 gaggaaagtc aaccttcgat aagggaacat tgacaatatc ggatgtttct aaagcttccg
     9541 cttcaatggc ctcagcctgc tcttccctta aaggagccac ggaaatgatt ttgccccgcc
     9601 ggatgtactg cagaatggcg cggacggcag ccatgcgggg attgatgacc gtatccaatc
     9661 cgacgttgga gacgacatgc atgtaactga gtttgctgac cctggtgatg gtgcgtgctg
     9721 ttcccaattc ctttgccaaa agcgagatca ggacattgct ttcttcatcg tcggttacgg
     9781 caatcagcat gtccattgcg cgggcattct cttcgataag caagtcccgg tcaccgccgt
     9841 ctcccttgat cacaacgact ttttcaagaa gcctgttggc ttccttgcac ttggccccgt
     9901 cctgatcaat caatttgaca ttcaaacgtc tttgatcgag cgctttggcc acggccatgc
     9961 ccgttcctcc cgctccgaca atgatcacat ttctcatcgg cttggcaggc atgtgaagac
    10021 gggagagtgt ctggtctaat tccgattttt tgatgacaaa ataaaccagg tccatgggtt
    10081 gaatggtatc attgccattg ggaataagga ttttatgtcc cctgacgatt gcgccaacca
    10141 gcaattttcc ttcctgattg ctcagctcgc tgagtttttt acccgccagc ggggaatcct
    10201 cgaaaatcag aaagctgacc aacttgacgg cgccccccag aaaatcaatc acctccgatg
    10261 cgcccggaat atccataagg ttgagaatgg tctcgaccat catggactgg gtattgatga
    10321 tcagatccac gcccagaaga tctttgctga ataatgattt ttcctgaagg tattcctgat
    10381 ttctgacacg cgctattttt accgcatatc tgttcaggtt gcgcgccagc aggcacgcaa
    10441 tgagattgac ttcgtccata tcggttgcgg ccacaagaag ttgcgcatcc tgaatgcctg
    10501 cctgccgcaa cattttggga cttgtgcccg atccaagaaa agcctggaca tcaaggtttt
    10561 cgttgatccg tctgatcttg cccggatcct tgtcaattaa gacaacgtct cttttttcat
    10621 cggaaagttt tttggcaatg tggtatccga cctctccggc gcccactata ataacgcgca
    10681 tatttccccc ctccacgtta caacggtatc aacttatata atggacaccc cgggaattac
    10741 aacataaaaa gcttgataac gccgccccag atcattcact ttacggacgg ctttttcgga
    10801 ttccagagtg accaggatga taggattttt aaaaacatat tgctcttttt tgaatgtgca
    10861 atttcttaat aatcatcaca taacatccac tgttcaaaag gccggtccgc atcgtattct
    10921 gaaagaggcg cgatttcccg gataaaccgg gagggagcca tattgagaac tcctgaggaa
    10981 cgcgtataat ccagggcagg atagcataaa tagagttgat ctttggcccg ggtcaccgcc
    11041 acatagaaga gccttctttc ctcttcctcg ccgtcctgct ctttcaacgc cttctgcaac
    11101 ggaatcatgc catccgcgca ccagatcaga aaaacatacg accactccag ccccttggcc
    11161 tgatgaatgg tgcttaaggc aaccttgtct tcatcgtcct cttccccgat gttctcttct
    11221 ttggccatat tggtcagaag cgccagttcg ttgaggaagt cctccatctg cagaaactgg
    11281 gcggaaaaat ttccgagctg aattaaatct tcctctctgg atgatgcatc ggaatagtta
    11341 tcctgaagat aaacccggta gccgcctctg tttaacaaca catcgataat cctttccgga
    11401 atccgttcat tttcaggaag atcgagaagc tgccggatcg ttttccggca ttcttccaga
    11461 ccgggttttg cggatttgga catcgccttg atgaagtcct cgctcaagac agcattcagc
    11521 ggattgtctt ccttcttcag ataatgccat attttattga aggtggcctt gcccaccttg
    11581 ggatacatga tcagcaacct acgccaggcc agttcgtcgc ggggattgac cagaaccctc
    11641 atgtaggacg ttacgtcttt aatatgcgcc tgttcaaaaa aacgaatgcc cgaacgcata
    11701 tcgaaaggaa tgtcgcgccg gacaaattcc atctgcattt ccatcgaatg ataatgcgcc
    11761 cggtaaagca cggcgatttc ccggagcggc acgccgcccc gcatcagttc cgtaacccgc
    11821 tgggccacaa aatccgcctg tttaacaaca ttctgcgcgg aaacaatcac cggcctcatg
    11881 ccgtttttgc gcacagcctt gagttccttg ggaaattgtt tttcgttatt ggtgatgctt
    11941 aaatttgcca gatgcagtat ttccggcgta ctgcgatagt ttgtttccag cttgaatatt
    12001 ttgcagtccg gataacgatg ggggaaatta atgatgttgt tgaaatcggc accacgaaag
    12061 gagtaaatac tctgggaatc gtctccgacc accatcaggt ttttgtgcgt agaggccagc
    12121 agatcgatca gatccgcctg aataagattg gtatcctgat attcatccac caggacatac
    12181 tgaaaccggc cggaaagatt tttcagaaca tcagcgtttt cctgaaggag catgcgacaa
    12241 ttgcttaata aatcgtcaaa atccatgacg gccagctccc tcttcttttg ttcataaaga
    12301 gaagcaactt gaataatttc atccatcaga tggagaaaaa acgagtatcg tgacatcaga
    12361 acattttcca cggtagtttc cgtgttgaca gacaggctga tgatgtcccc tacgatatta
    12421 ctcttgggga acttgcttaa tttcgggtct atttttaatt cggccataca ggtggaaatg
    12481 atctgtcggg catcctcact gtccacaatc gaaaaatttg ttttatagcc aaggtaatgc
    12541 gcatgccttc tcaaaatcag atgggcaaca tggtgaaatg tgccgcccat gatatttctc
    12601 gtctcggcgt gaatcaggct ctccacccgg ttgagcatgg catgagaggc cttattggtg
    12661 aaagtggcca gtaaaatgtt ttcctgcgca acgccgcttt ccagcaggcg ggcgactcga
    12721 taagtaagcg ttcgggtttt tccgcttccg gccccggcca gaaccagcag cggaccgccc
    12781 tcttccatga ccacacgata ctgttccgga ttcagttctt ttgcataatc aatcatcaac
    12841 gcgtctttat agtaaaaggt tcagatattc aagcgaataa cggcaa
//
LOCUS       DAOOLB010000059        12793 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_5405_length_12793_cov_40.023552, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000059 DAOOLB010000000
VERSION     DAOOLB010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 12793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12793
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5405_length_12793_cov_40.023552"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..710
                     /locus_tag="PLF77_07880"
     CDS             54..710
                     /locus_tag="PLF77_07880"
                     /inference="COORDINATES: protein motif:HMM:NF004981.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase
                     domain-containing protein"
                     /protein_id="HPC08811.1"
                     /translation="MIDLHTHSIFSDGELIPAELAQRAWAAGYKFLAITDHADHSNID
                     FIIPRIIRACFKINQAGKINVFPGVEITHVAPAQIAGLANEARKLGAKVVVVHGETIV
                     EPVPAGTNLAALKADIDILAHPGLLTEKEARLAAERGIYLEITTRRGHSFTNGHVAQT
                     ARKCKAKLMLNNDAHAPADFAGRERAQRIASGAGLNAREVSAMFENSRRMVKKLQYER
                     "
     gene            700..1959
                     /locus_tag="PLF77_07885"
     CDS             700..1959
                     /locus_tag="PLF77_07885"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPC08812.1"
                     /translation="MKGKSKSQPLKICLLTYRGNPNCGGQGVYIKYLSKALSDLGHEV
                     DVLSGPPYPHLDPPVRLHRLPSLDLYNVEHPFRVRNARALMNPLNMYEFLTMCTGRFP
                     EPYTFGVRAYNYLQRHRKKYDIVHDNQCLAYGIGKIAQNVVPTIATIHHPITVDRQEE
                     FKSATSKRKINKIKKWYSFIRMQKEVARQLSHIVTVSECSKIDIAEEFSIPSSKFRVV
                     PNGINKEFFYPVQNGSRPENSIIVTNSADTPLKGLRYLLEAVYQIRKKQPVKLTVIGE
                     PKKNGVVKKLVAELGIEDIVHFTGRIANEEFADYYSKATIAVVPSLYEGFGLPAAEAM
                     ACGVPLVSTSGGALPEVVGDAGIIVPPADADALAREILFLFNHPDQRKKMAQAGIARV
                     DAIFNWSKAAGDMVEVYREAIHDYRGN"
     gene            1940..2641
                     /locus_tag="PLF77_07890"
     CDS             1940..2641
                     /locus_tag="PLF77_07890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC08813.1"
                     /translation="MITVEIDKLDLKPDSVVLDAGCGLGRHLRHLARIPDLKIFGIDK
                     NTWALQETAKSMDTQPDARSKDYLVSIADINRLPFANASFDCIICSEVLEHIPDHQKA
                     IQELDRILKPNGTLVVSVPRYFAERICWFISRDYHNEEGGHIRIYRKKQLHRMLTGQG
                     FECWKINYKHALHSPYWWLKCLVGLKNEDHPLVRSYRKFLEWDIMKKPRPVRMLEELL
                     NPLMGKSIVYYLRKG"
     gene            2647..3681
                     /locus_tag="PLF77_07895"
     CDS             2647..3681
                     /locus_tag="PLF77_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08814.1"
                     /translation="MELELSVSEASAPVSVEKIGSFIISLQKENGEIPWSAGGKTDPW
                     DHVESAMGLSAAGFYTEAEKAYEWLASTQLEDGSWWSEIQDGKIINSCKESNFAAYVA
                     VGVFHHYLVTRDEKFLKKMWHTIAQGIQFAINLQAPDGEIYWARNKHGAADKMALLTG
                     SSSIYMSIKCALAVADRLGKKRAGWRKAMDALGAAIKNRPDLFNMIKSRYSMDWYYPI
                     LCGAVTGAEAQKRIAHLWEKFVVPDWGVRCVSDRPWVTMAETAEFILTLASIGDLARA
                     KTIFSWLHDKRFSDGSFWMGVTFPDGVIWPEEKTGWTAAAVILASDALNEMTPAGKLF
                     NHQFWASISK"
     gene            3776..4498
                     /locus_tag="PLF77_07900"
     CDS             3776..4498
                     /locus_tag="PLF77_07900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPC08815.1"
                     /translation="MRKDHRPYYIKRFYNRFQKWYARYYIAPQCEYFGRGLVFMKPWH
                     VEIFGGPVSLGDYTTVIATSDRKVRFTVWPVWMGQGKIEIGQCCLICPGTRIMSATSV
                     TMGDGCMTAQSVSIGDADWHDIYDRSMPVGNTQAVTIGNNVWIGDSAMVSKGVTIGDN
                     AVIGAGSVVVKDIPANAIAAGNPARVIKYLDPDRPIRTRKDWLSDPAGLARDFEVIDR
                     AIMKGNTMAGWFRSVLFPRQGD"
     gene            4502..6076
                     /locus_tag="PLF77_07905"
     CDS             4502..6076
                     /locus_tag="PLF77_07905"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08816.1"
                     /translation="MRIAVIAPPYPLEEMPSPPLGVTYVAAAFAGAGADVRIFDYIVS
                     GYSREKIENQLASFSPDAVGATSVTLNFYDAQKILRDVKQYNHEIITIMGGPHVSFTV
                     EETLKHYPEIDLIVIGEGEATIAELTPVLRQKEKWREVRGIAYREGDEIVRTPKRDFV
                     TDIDNIPLPARHLLPISRYRALGFPVSLITGRGCPYSCIFCLGRKMVGSKVRKRNSRL
                     VLDEIEQIIGYGFDRINIADDLFTTDKERVREICAGIKERSLKFPWSAFARVDTVSQE
                     VFDLMAEAGCDSVSFGVETGNPDMLKRIKKGIRLEQVYSAVKMCQQAGMIAHASFIIG
                     LPGETKETLHQTDQFAKSTKAIYGYHFLAPFPGTTVREKIGDYDLQILTDDWSRYDAN
                     DAIVRTSFLNPEEMKAFGDGYEKEMTDDWNKIVRRYHEGRGSVEDKMQVEGHWRMKLT
                     YRLLKEDIIEQCGLIEPECCRGSKENALRELCNRIGKITQADPGVIDNTLYDFAGRGY
                     LKAEMSDQGCRWSWSK"
     gene            complement(6079..6699)
                     /locus_tag="PLF77_07910"
     CDS             complement(6079..6699)
                     /locus_tag="PLF77_07910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC08817.1"
                     /translation="MNISKQKIDQVKGFLDEREAACLYRLALEAGKKGPCLEIGSYCG
                     KSSVYLGTACKENGTVLFSLDHHTGSEEQQPGEEYFDPDLLDRESGKIDTFKFFRKTI
                     FDFDLDDVVIPVVGRSATIGRVWKTPLSLIFIDGSHAYDSVLSDYEIWEKNLMPGGYL
                     LFHDIFSDPAKGGQAPYRVYQQAVDSGRYTEMPMCESLGILIRKQE"
     gene            complement(6815..8155)
                     /locus_tag="PLF77_07915"
     CDS             complement(6815..8155)
                     /locus_tag="PLF77_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC08818.1"
                     /translation="MKKILFTLLMVFLFFTPSIFAEMVQDIPAGTAEDYYNRGNKYRE
                     QGNLDQAIYDYSKAIKANSKYSKAYYNRANSYVKQGKLAEAIEDYTKAVEINPLYTEA
                     YYNMGNTYEKQGDLAQAVASYTKALETNPRYSAAYCNRGNVYQAQGNFQQALDDYNKA
                     VELNPNFAGVYSNRGNVYQAQGNTDKAIADYNKAIELSPNFAGFYANRGNIYQSQGNL
                     QQAIADYNTAIEKNPDDTSSFYNRGLAYYGLEEYAKSLADYSTALNQNPDKEAYDDFL
                     KYFPEKRASDNQDIRGAIVQVFEDKLNLPKKIAAPVAAAPADAIPPAAAAAVVADTKV
                     SSTPEEDIKGSVNTWLNSWQSGDMATYRSCYDERFESRKMNLDAWIDYKNNVRNRSKN
                     ISIQIDDLKISVDGDRAKAVFTQSYSSSLLKDKGTKTLELKKVGDNWKIYREIM"
     gene            8602..>10307
                     /locus_tag="PLF77_07920"
     CDS             8602..>10307
                     /locus_tag="PLF77_07920"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPC08819.1"
                     /translation="MANCESGYAKKLLEINLTEAKIQKAPLDESLLRNYLGGGGLAAH
                     LFLNRFTPDSDALSADNPLIIMTGPLVGTLLPGTSRFAVCARSPQTGFWGIGTCGGTF
                     GPELKFAGYDGIFIEGKSEKPVYLLIEDDNVEIRDAADLWGEPVYKVTDILKERHGGK
                     RKPKVLVIGPGGENLVRFAAIANDKAHYIGRTGMGAVMGSKMLKAVVVSGTGKVLPAL
                     PEEYKALRESLLEKIKENQFGQVLRSMGTAASMDPGMMMGDVPIRNWTKGEFDGSVNI
                     GGPTMTEKYLTKVHACYACPIACKRVVRVEGGPFQTEEGPGPEYETCCTFGSFMENDN
                     LEGIIKANETCNQYGVDTISCGATIAFAMQCFDSGILSTEDTDGIELNWGNIESALKM
                     VEKIAKREGIGNVLAEGSAKAAQIIGKGSEQYVVAVKGLELPAHDPRGFHGMGLEYAV
                     GYRGACHLQHMSHTIEQGMTDQADAGFKADYEGPSSDGKAEMVVLSQNIGVPPTSACL
                     CLFVYDCIAVKDFADMIHVVTGWDYSIDDLIKTGEKIWLLMRGITNLLGSRADDDTLP
                     PNV"
     assembly_gap    10308..10407
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10457..11620
                     /locus_tag="PLF77_07925"
     CDS             10457..11620
                     /locus_tag="PLF77_07925"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPC08820.1"
                     /translation="MAYDRDLNFIYRNPTRLIYGENSINEVGLEADALKCSRAFLVTD
                     KGIVNAGLTERVEKALGRKLVGTFDGCIQDSDLRIINDAAEIARGKGADILVSVGGGS
                     VIDTTKGIAIVLKEGGKIEDYKGQQCLTRPQTPHIVIPTTAGTGSEVTYFAVIKDQVN
                     HQKHPYCDDNIIPNVGILDPTMTAGLPPMLTATTGMDAFSHALEAIHATPCSPVADGM
                     ALHAIRLIMEYLPLCVENGKDLFARGQQLMASTIAGIAFTNAQVGLVHAIAHTLGGLF
                     QVPHGLANSLMLPHVVRYNMDTCADKYVMIAQAMNIYAKGMSDEEASEAVAEAITALT
                     KRMGVPQRLRDVGVPEDGLVQLAEGTLSDGAIVYNPKPVFEAEEVLGIIKQAW"
     gene            11664..12086
                     /locus_tag="PLF77_07930"
     CDS             11664..12086
                     /locus_tag="PLF77_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08821.1"
                     /translation="MAQINFAPGVEDAGLPVMIADIIKGNLEAKPQRQGDFNALSGNI
                     YMQAHDAEVDMTLAFEKGNLTVHSGKVGDPKISISTDSDTLLALANIDVKFGLPYYFD
                     KVGFEVLKKLFLTRELKLAGLLTHPIMLTRFTKLMSCR"
     gene            12438..>12793
                     /locus_tag="PLF77_07935"
     CDS             12438..>12793
                     /locus_tag="PLF77_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08822.1"
                     /translation="MREIHAGGKLLKKIYVAIFLWFTGRAIQAAAKVDKEVQKEFDAL
                     PDEFTFALGCLPNGPWMVVGKQKGKVKYFGGDLKCRPVHLRLVIKSIDAAFLILSFQE
                     STALATTRNRLMTDGEV"
BASE COUNT         3199 a         2936 c         3432 g         3126 t
ORIGIN      
        1 catcagatta agcattctaa atctgtcgta tatttagaat caggattttt taagtgattg
       61 atctgcatac tcattccatt ttcagcgatg gcgagttgat acccgcagaa ctggcgcagc
      121 gagcctgggc cgcgggttat aagtttctgg ccattaccga tcacgcggat cattccaata
      181 tcgatttcat cattccccgg atcatcaggg cctgtttcaa aattaaccag gcgggaaaga
      241 tcaacgtttt ccccggagtc gaaatcacgc atgttgcgcc cgctcagatc gccggtttgg
      301 cgaatgaagc cagaaaactg ggggcgaagg tggtggttgt acacggggaa acgattgtgg
      361 agccggtgcc tgccggaaca aatctggctg ccctgaaagc agacattgat atcctggcgc
      421 atcccggact cctgacggaa aaagaagcgc ggcttgccgc cgaacgggga atttatctcg
      481 aaatcacaac gcggagaggt cacagtttca ccaacgggca tgtggcgcag acggcaagga
      541 aatgcaaagc gaagctgatg ctcaataatg atgcccacgc gccggctgat tttgccggcc
      601 gggaaagagc gcaacgcatt gcaagcggcg ccggactgaa tgcaagagaa gtatccgcta
      661 tgtttgaaaa ttcgcgaagg atggtaaaaa agttgcagta tgaaaggtaa atccaaatca
      721 cagccgttga aaatttgtct gctgacctac cggggcaatc ccaattgtgg cgggcaggga
      781 gtatacatca aatatttgag taaggcactc agtgatctgg ggcatgaagt cgatgtgttg
      841 tcgggaccgc cttatccgca tctggatcca ccggtgcgtc tgcatagact tcccagcctg
      901 gatctttata atgtcgaaca tccctttcgg gtgcgcaacg cgcgcgccct gatgaatccg
      961 ctgaatatgt acgaatttct gaccatgtgt acgggccggt ttcccgaacc gtatactttc
     1021 ggcgtccggg cttataatta tctgcaacga caccggaaga agtatgatat cgtccacgat
     1081 aaccagtgtc ttgcttacgg gatcggtaaa atagcgcaga acgtcgttcc cacgatagcg
     1141 acgattcatc atcccatcac cgtcgaccgt caggaagagt tcaagtcggc gaccagcaaa
     1201 aggaaaatta ataagatcaa aaaatggtat tccttcatcc ggatgcagaa agaagtcgcc
     1261 cgccagcttt cgcatatcgt gacggtttcg gaatgttcca aaatagacat tgctgaggaa
     1321 ttttccattc cgtcgtccaa gtttcgcgtg gtgcccaacg ggatcaataa agagtttttt
     1381 tatccggttc agaacggatc gcgtcctgaa aactccatta ttgttactaa cagcgcggat
     1441 acgcctttga aagggctgcg ctatctgctg gaagccgttt accagatcag aaaaaaacag
     1501 ccggtgaaac tgacggtaat cggagagccg aagaaaaacg gtgtcgttaa gaaactcgtc
     1561 gccgaactgg gtatcgaaga tatcgtccat tttaccgggc gcatcgccaa tgaagaattt
     1621 gccgattatt attccaaagc cacgattgcc gttgttccgt ctttatatga aggattcggc
     1681 ctgcccgcgg ccgaggcgat ggcgtgcggc gtgcctctcg ttagcaccag cggcggcgcc
     1741 ctgccggaag ttgtgggaga tgccggaatt attgtacctc ccgcggatgc agatgctctg
     1801 gccagagaaa tcctgttctt gttcaatcat cccgatcaaa gaaaaaaaat ggcccaggcc
     1861 ggtatcgcaa gggtcgatgc cattttcaac tggtcaaaag ctgccggcga catggtggaa
     1921 gtgtaccgag aggcaataca tgattaccgt ggaaattgat aaacttgatt taaagcccga
     1981 cagcgtcgtt ctcgatgccg gctgcggact gggcagacat ctgcgtcatc tggcgagaat
     2041 tccggatctg aaaatattcg gcatcgataa aaacacctgg gcgcttcagg aaaccgcgaa
     2101 atccatggat acgcagcccg atgcccggtc gaaggattat ctggtgtcca ttgccgatat
     2161 caaccggctg ccttttgcaa acgcttcttt tgactgcatc atctgttcgg aagttctgga
     2221 gcatattccc gatcatcaaa aagccatcca ggagctggac cgaatattga aaccgaacgg
     2281 gacgctcgtg gtcagtgtgc cgcggtattt cgccgaaaga atctgctggt ttatttcccg
     2341 cgattatcat aatgaagaag gcggccatat ccggatttac agaaaaaaac agctgcacag
     2401 aatgctgacg ggccagggat tcgagtgctg gaaaatcaat tacaagcatg cgttgcattc
     2461 accgtactgg tggctcaagt gcctggtggg cctcaaaaat gaagatcacc cgctggtcag
     2521 aagttatcgg aaatttctgg agtgggacat tatgaaaaaa ccgcggccgg tgcggatgct
     2581 cgaagaattg ctgaatccgt tgatgggcaa gagcattgtc tattatctta gaaaaggttg
     2641 attggaatgg aacttgaact ctccgtcagt gaagcgagcg ctccggtctc tgtggaaaaa
     2701 atcggttctt ttattatttc gcttcaaaaa gagaatggcg agatcccctg gtcagccggc
     2761 ggaaagaccg atccgtggga tcatgtagaa agcgccatgg ggctttccgc ggccggtttc
     2821 tacacggagg cggaaaaggc ctatgaatgg ctggcgtcga ctcagcttga agacggcagt
     2881 tggtggtcgg aaattcagga cggaaaaatt atcaattcct gcaaagaatc gaattttgcc
     2941 gcctatgttg cggttggcgt cttccatcac tatctggtga cgcgcgatga gaagttcctg
     3001 aaaaagatgt ggcataccat tgcgcagggg attcagtttg cgatcaatct gcaggcgccc
     3061 gacggcgaaa tttactgggc gagaaacaaa cacggcgcgg cggataaaat ggcgttgctg
     3121 accggctcca gttccatata catgagtatt aaatgcgcgc tggcggttgc cgaccggctg
     3181 gggaaaaaac gcgcgggctg gcgcaaggct atggacgcgc tgggcgcggc aattaaaaac
     3241 aggccggatc tcttcaacat gatcaagtcg cgctattcga tggactggta ttatccgatt
     3301 ttatgcggag ccgtcaccgg tgcggaagcg cagaaacgaa tcgcccattt atgggaaaaa
     3361 tttgttgttc ccgactgggg cgtgcgctgt gtcagcgacc gtccgtgggt gaccatggcc
     3421 gagacggcgg aatttattct gacgctggcg tcgatcggcg atcttgcccg ggcgaaaacc
     3481 atattcagct ggcttcatga taaacgattt tccgacggct cgttctggat gggggtgacg
     3541 tttccggatg gcgttatctg gccggaggaa aaaacgggct ggacggcggc agccgtcatt
     3601 ctggcttcgg acgctctgaa tgaaatgacg ccggcaggaa agcttttcaa tcatcaattc
     3661 tgggcgtcga taagcaagtg aaaatgcttt ttgcaaagtg gctgtttccg atgatgtccc
     3721 cgcccggttt ttaaaaaata tctgctacaa taatccaata tcaaaaggtt cagcagtgag
     3781 aaaagatcac cgtccatatt acattaaacg cttctataac aggtttcaga agtggtacgc
     3841 gcgttattat atcgctccgc aatgcgaata tttcggacgc gggctggtat ttatgaagcc
     3901 gtggcatgtg gaaatattcg gcgggccggt ttccctgggg gattacacga cggtgattgc
     3961 cacctccgac cggaaggtgc ggtttacggt gtggccggtc tggatggggc agggcaagat
     4021 tgaaatcggc caatgctgcc tgatctgtcc cgggacacgc atcatgtccg cgacatcggt
     4081 taccatgggt gacggttgca tgacggccca aagtgtcagt atcggcgatg cggattggca
     4141 tgatatttac gatcgcagca tgccggtcgg caacacgcag gcggtgacga tcggcaacaa
     4201 tgtctggatc ggcgacagtg ccatggtttc caaaggggtg acgatcggcg ataacgccgt
     4261 catcggcgcc ggatcggtgg tggtgaagga tattcccgcc aacgccatcg cggcaggcaa
     4321 tccggccagg gtcatcaaat atcttgatcc ggacaggccg atcagaacga gaaaagactg
     4381 gctgtccgat cccgccgggc tggcccggga ttttgaagtg attgatcgtg caattatgaa
     4441 gggaaatacc atggccggct ggttccggtc cgtgctgttt cctcgtcagg gagattgaaa
     4501 aatgcgtata gctgttattg ctccgccata tccgcttgaa gaaatgccgt cgccgccgct
     4561 gggcgtgacg tatgtggccg cggccttcgc cggtgccggt gcggatgtcc ggatctttga
     4621 ttatattgtg tccggatatt cccgtgaaaa aattgaaaac caactggcgt cgttttcacc
     4681 cgatgccgtc ggggcgacat cggtaacctt gaatttttac gatgcgcaga agattctgcg
     4741 tgatgttaaa cagtataatc atgagatcat caccatcatg ggcgggccgc acgtatcgtt
     4801 cacggtcgag gaaacgctga agcattatcc ggaaatcgat ctgatcgtca tcggcgaagg
     4861 cgaggcaacc attgccgaac tcacgcccgt gctcaggcag aaagaaaaat ggcgcgaggt
     4921 gaggggaatc gcctatcggg aaggcgatga gatcgtccgc acgcctaaaa gagatttcgt
     4981 aacggacatt gataatattc ctctgccggc aaggcattta ttgcccattt cacggtatcg
     5041 cgcattggga tttcccgtca gtctgatcac cggtcgcgga tgtccttatt cgtgcatttt
     5101 ttgtctggga cgcaaaatgg tcggctccaa agttcgcaaa cgaaactccc gcctggttct
     5161 ggatgagatc gaacagatca tcggttacgg tttcgaccgc attaatattg ccgatgatct
     5221 ctttaccacc gacaaggaaa gggtccggga aatctgtgcg ggaatcaagg aacgatcgtt
     5281 gaaatttccc tggagcgctt ttgcccgcgt ggatacggtc agccaggagg tctttgacct
     5341 gatggccgaa gccggatgcg acagcgtcag tttcggcgtg gaaacgggaa atccggacat
     5401 gctcaaacgg atcaagaaag ggatccggct ggagcaggtc tattcggcgg tgaaaatgtg
     5461 tcagcaggcg ggcatgattg cccatgcttc ctttattatc ggactgccgg gagagacgaa
     5521 ggaaaccctt catcaaaccg atcagtttgc caagagtacc aaagcgattt acggttacca
     5581 tttcctggcg ccttttccgg gaacgacggt gcgggagaag attggcgact atgatctgca
     5641 gatcctcacg gacgactggt cgcgctacga cgcaaacgac gcgattgtca gaacatcctt
     5701 cctgaatccc gaagagatga aggcgttcgg agacggctat gaaaaagaaa tgacagacga
     5761 ctggaacaaa atcgtccgga gatatcacga aggccggggc tccgttgaag ataaaatgca
     5821 ggtagaagga cactggcgga tgaaactgac ctatcggctt ctgaaagagg acatcatcga
     5881 gcaatgcggc ttgatcgagc cggaatgctg tcggggctcg aaagaaaacg cgttgcggga
     5941 actttgtaac agaatcggaa aaatcaccca ggcggacccc ggcgtgattg acaataccct
     6001 ttacgatttt gccggacgcg gatatttgaa agctgaaatg tccgatcagg gatgccgctg
     6061 gtcctggtcc aaatgaaatt attcctgctt gcggatcagg attcccagtg attcgcacat
     6121 gggcatttcc gtgtaacgtc cggaatctac cgcttgctga taaacccggt aaggcgcctg
     6181 tccgcctttc gccggatcgg aaaagatatc atgaaataaa agatatccgc cgggcatcag
     6241 atttttttcc cagatttcgt aatcgctgag cacggagtca taggcgtggc tgccgtcaat
     6301 gaatatcagg ctcaggggcg ttttccacac gcggccgatg gttgccgaac ggccgactac
     6361 gggaatgaca acatcgtcca ggtcaaaatc gaaaatggtt ttccggaaga atttaaacgt
     6421 atcgatcttt ccgctttccc tgtccagcaa atcgggatcg aaatattctt cgcccggctg
     6481 ctgttcttct gaaccggtgt ggtgatcaag ggagaacaaa acggttccgt tttctttaca
     6541 ggccgtgccc agatagacgg aagacttgcc gcagtagctt ccgatttcga gacaaggtcc
     6601 cttcttgccg gcttctaagg ccagacggta cagacaggcc gcttcgcgtt cgtccagaaa
     6661 tcctttgacc tgatcgatct tctgtttgct tatgttcatt gctgacgttg ccggatcaat
     6721 gaaaatggca aatgcttttt aaaaagcatt tgccatttgg ttttatgtta caatggactt
     6781 ttatgtcaaa acgcgcttca tcccaccgtc cgatttacat gatttcccga tagatcttcc
     6841 agttgtcgcc cacttttttg agctcaaggg ttttggtccc tttgtctttg aggagcgagg
     6901 aactgtagga ttgcgtaaag acggcctttg cgcgatctcc gtccacggat attttcaaat
     6961 catcaatttg aatgcttata ttcttacttc tgttgcgtac attattttta taatcgatcc
     7021 aggcatccag attcattttc ctggattcaa aacgttcatc gtaacaactg cggtaagtcg
     7081 ccatgtctcc cgattgccag ctgttaagcc acgtgttgac ggaacccttg atatcctctt
     7141 caggcgttga agacaccttt gtgtcggcaa cgactgctgc cgcggcagcc ggcggtatcg
     7201 catcggcggg agcggcggcg acaggagcgg ctattttctt cggcaaattt aatttatctt
     7261 cgaagacctg aacaatggcc ccccggatat cctggttgtc ggacgctctt ttttccggga
     7321 aatatttaag aaagtcatca taggcttcct tgtccggatt ctgattaagc gccgttgagt
     7381 aatcggctag cgattttgca tattcttcca ggccgtaata agccagccct ctgttgtaga
     7441 aggaggacgt atcatcggga tttttttcga tggcggtatt atagtcggcg atggcctgct
     7501 gaagatttcc ctgactctga taaatattgc cccggttggc gtaaaatccg gcaaaattcg
     7561 ggctgagttc gatggctttg ttgtagtcgg caatggcctt gtcggtgttg ccctgtgcct
     7621 gatacacatt gcctctgttg gaatacacgc ccgcaaaatt cgggttaagc tctacggctt
     7681 tgttgtaatc gtcaagcgcc tgttggaaat taccctgtgc ctgatacaca ttgcctctgt
     7741 tgcagtatgc ggccgagtat ctgggattgg tttccagagc ctttgtataa ctggcgactg
     7801 cctgagccag gtcgccctgc ttttcgtaag tattgcccat gttgtagtag gcttccgtgt
     7861 acaggggatt gatttccaca gcctttgtgt agtcttcaat ggcttcagcg agtttgccct
     7921 gtttgacata ggaattggcg cggttgtaat aagccttgga gtatttggag ttcgccttga
     7981 tcgctttgga ataatcgtag atagcctggt cgagattacc ctgttctctg tatttatttc
     8041 cccgattgta gtaatcttcc gcggttcccg cggggatatc ctggaccatt tcggcaaaaa
     8101 ttgatggcgt gaaaaataaa aataccatta acaaagtaaa taatatcttt ttcataatta
     8161 ttgccttcct ttttttaaat taattttggt tgatatcctt gaaaagtgca gaaaattaaa
     8221 caaaaaatgt tggtgttgtc aagctaattc ttgccgggaa cgtgattcat caggtttgtt
     8281 gcgagccttg aaacagcaaa agacggctga tacatcaaat gttagaaaaa gcataaagga
     8341 ccgttgaaaa aaattaatct gtttgaattt catcttgtgt aacaaatcga attgtgttag
     8401 aagcggcaca ttctgtaaaa aattgtcatt cagtgtagtt cataaatctg gggggatttt
     8461 atgaaaacac cgctgtatta ttgaagatgt tgaagcatct gcaaggctgt tgaatgtctt
     8521 cttcaataca cgctcttatt attcaggaaa agttcccata attacaagca ggaaactgaa
     8581 ttattgtttt caggagacgg tatggcaaat tgtgaatcag gatatgcaaa aaaactgctg
     8641 gaaatcaatc ttaccgaggc aaaaattcaa aaggcgcctc tggatgaatc tcttttaaga
     8701 aattatttgg gaggaggcgg tctggcagcg cacctctttc tcaaccgctt tacccctgac
     8761 tccgatgccc tctccgctga caatccgtta atcatcatga ccggaccgct tgtcggaaca
     8821 ttgcttccgg gcacatcccg ttttgccgtg tgtgcccgat cgccgcaaac cggtttctgg
     8881 ggaatcggca catgcggcgg tacgttcgga ccggaactca agtttgccgg atacgatgga
     8941 atttttatcg aagggaaatc agaaaaaccg gtttatcttt tgatcgaaga tgacaacgtg
     9001 gaaatccgcg acgctgcgga tctgtgggga gaaccggttt ataaggtgac cgacatatta
     9061 aaggaacggc atggcggcaa gcgcaaaccg aaagtgctgg tgatcggacc cggtggagaa
     9121 aatctggtgc gctttgcggc cattgccaat gacaaagcgc attacatcgg cagaaccggt
     9181 atgggagccg tcatgggctc taaaatgctt aaagccgttg tggtcagcgg tacaggcaag
     9241 gttttgccgg ctttaccgga agagtacaaa gcccttcggg aaagccttct tgagaaaatc
     9301 aaagaaaacc agttcggcca ggttttgcgc agcatgggaa cggcggcttc gatggacccg
     9361 ggaatgatga tgggagacgt gccgatccgt aactggacca agggtgagtt tgatggctcc
     9421 gtgaacatcg gcggaccgac gatgaccgag aaatacctga ccaaggtgca tgcctgctat
     9481 gcctgtccga ttgcctgcaa gcgcgtcgtc agagtggaag gcggaccgtt tcaaaccgaa
     9541 gaggggcccg gtccggagta cgagacctgc tgcacatttg gctcctttat ggaaaatgat
     9601 aatctggagg ggattattaa ggccaatgag acctgcaacc agtacggtgt agataccatt
     9661 tcctgcgggg ccacgattgc ctttgcgatg cagtgctttg atagcggtat tctttccacg
     9721 gaggataccg atggaatcga actgaactgg ggaaatatcg aatccgctct gaaaatggtc
     9781 gaaaagattg ccaagcgcga gggcatcgga aatgtcctgg cggaaggttc cgctaaggcg
     9841 gcgcaaatta tcggcaaggg ttccgaacag tatgtggtgg cggtcaaggg actggaactg
     9901 ccggcgcatg atccccgcgg gtttcacggt atgggactgg aatacgctgt gggatatcgc
     9961 ggcgcctgcc accttcaaca catgagtcat accattgaac agggaatgac ggatcaggcg
    10021 gatgccgggt tcaaagccga ttatgaaggt ccctccagcg atggaaaggc ggagatggtt
    10081 gtcctttctc agaatatcgg cgtgccgccc acgtcggcct gtttatgtct ttttgtgtat
    10141 gactgtattg ccgttaaaga ttttgcggac atgattcatg tcgtgacagg ctgggattat
    10201 tccatagacg atttgatcaa aaccggagaa aagatctggc ttttgatgcg cgggataacg
    10261 aatctgctcg gctcacgggc ggatgacgac acgctgccgc cgaatgtnnn nnnnnnnnnn
    10321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnnnnn nnnnnnnnnn nnnnnnntca atatgctcat aaattaatga ctcaatcatt
    10441 caaggagagg tgaactatgg cgtacgacag ggatctcaac ttcatctatc gcaatccgac
    10501 aagattaatt tacggggaga attcgatcaa cgaagtggga ctggaagcag atgcgctgaa
    10561 atgcagcagg gcctttttgg tgacggataa gggcatcgtg aatgccggac tgaccgaacg
    10621 ggtggaaaaa gcgctgggca gaaaactggt gggaactttt gacgggtgca ttcaggattc
    10681 cgatttgcgc attattaatg atgcagccga aattgcccgg ggcaagggag ccgatattct
    10741 ggtcagtgtc ggcggcggca gcgtgattga tacaaccaaa gggattgcta ttgttttaaa
    10801 ggaaggcggt aaaattgagg attataaggg gcagcagtgt cttacgcgtc cgcaaacgcc
    10861 ccatattgtc attcccacaa cggcaggcac cggcagtgag gtgacgtact ttgcggtgat
    10921 caaggatcag gtcaaccacc agaaacaccc ctattgcgat gataatatca ttcccaatgt
    10981 cgggattctc gatccgacca tgaccgcggg attgccgccg atgctgacgg ccaccacagg
    11041 catggacgct tttagtcacg cactggaggc cattcatgcg acaccctgct cgccggttgc
    11101 ggacggcatg gcgctgcacg ccatccgatt gattatggaa tatctgcccc tgtgcgtaga
    11161 gaacggcaaa gacctgtttg cgcgaggtca gcagttaatg gcgtctacca tcgccggaat
    11221 cgcctttacc aatgcccagg tcggtcttgt ccacgccatc gcccatacgc tgggtgggct
    11281 tttccaggtg cctcacggat tagccaacag cctgatgctt ccgcatgtcg tccgttacaa
    11341 catggacacc tgcgcggata aatatgtcat gatcgcccag gccatgaata tttacgctaa
    11401 ggggatgagt gatgaagagg cgagcgaagc ggtggccgaa gccataaccg cattaaccaa
    11461 aagaatgggc gtaccgcaac ggttgaggga tgtcggtgtt cctgaagacg gtctggttca
    11521 gttggctgaa gggacgttat ccgatggcgc cattgtttat aatcccaaac ctgtttttga
    11581 agcggaagag gttctcggca tcatcaagca ggcatggtag tttggattca ttaatattca
    11641 ttcaaagata tgaaggagga gctatggctc agatcaattt tgcccccggc gtggaggatg
    11701 ccggtctgcc cgtcatgatt gctgatatca tcaagggcaa tttggaggcc aaaccgcagc
    11761 ggcagggcga ctttaatgcc ctcagcggca atatctatat gcaggcgcat gacgcggaag
    11821 tcgacatgac cttggccttt gaaaaaggca atctgaccgt gcacagcggc aaggtgggag
    11881 atcccaagat atccatcagc accgattcgg atacactgct ggcactggcc aatattgatg
    11941 tcaaattcgg cctgccctat tatttcgata aagtcggttt cgaagtgttg aagaagctgt
    12001 ttttaacccg ggagcttaaa ctcgcaggac tcttaactca cccgatcatg ctgaccagat
    12061 ttacaaagct catgtcctgt cgttgaagcc tgccaaacgc catcgataga tcattttaaa
    12121 aattttaaca acagcgcgcg cattcaggcg cgttgttgtt aaaaaccgtc ttattgcgtc
    12181 tgacgacccc aaaaaaatta ctttgacttc aaatcgaaaa ttgtttatat cgtaaaaaaa
    12241 aatggttcta atctaaatcg catcacagca aaaaagaaga ctcatgtccg ttataattcg
    12301 cattagattc tttggtttct tatcaatgag tgtcatgccc gttgtaatga atgttagatt
    12361 caataatctc tagatatgtt aaagcatatg cagcatcatg ctgttttcca agagaagata
    12421 agggggagga tttgattatg cgggaaattc atgcgggagg gaaacttctg aaaaaaattt
    12481 acgtggctat attcctgtgg tttaccggca gagccattca ggcggcggca aaagtcgaca
    12541 aggaagttca aaaagaattt gatgctcttc ctgacgaatt cacctttgcg ctgggttgtc
    12601 tgcccaacgg tccgtggatg gtggtcggca agcaaaaggg caaggtaaaa tatttcggcg
    12661 gcgatctcaa atgccggcct gttcatcttc gtctcgttat caaaagtatt gatgccgcat
    12721 ttttaatatt gtcttttcag gaaagcacgg ctctggcgac aacccgtaat cgtctgatga
    12781 cggacggcga agt
//
LOCUS       DAOOLB010000060        12307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_5688_length_12307_cov_53.592067, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000060 DAOOLB010000000
VERSION     DAOOLB010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 12307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12307
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5688_length_12307_cov_53.592067"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="PLF77_07940"
     CDS             complement(<1..533)
                     /locus_tag="PLF77_07940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HPC08823.1"
                     /translation="MTVPRIYSPESLENKNSCALGEDNVKYLKKVMRLKEGDRINIFD
                     GFGREYEATIKNFSAKSVLIDLGKAIPSVNRDISITLAQAIPKGKKMDTIVKSAAELG
                     ADMIIPFHAVRSVSHIAEEKNPLKQTRWQKIAHEAARASHSVYITEVTKILSFPDMLS
                     RCSPKALKLIFWEEESR"
     gene            complement(589..1530)
                     /gene="prmA"
                     /locus_tag="PLF77_07945"
     CDS             complement(589..1530)
                     /gene="prmA"
                     /locus_tag="PLF77_07945"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPC08824.1"
                     /translation="MTEKETGKWLKIEILAPPELMEALGNFLTESGVQGVFQESQSSQ
                     AVGSDFPEPVAEDILKAYFPLDNRSDKKLAALQKYIDSLAEIFPDLGKPSFTTETICD
                     PDWGEQWKKYFKPIRVSNNIVVKPTWERYTPSSRDIVIEIDPGMAFGTGQHASTRMCI
                     EAVEDIIMNDRSIKEWKVLDVGCGTGILGITAAKMGAQDVLCADIDRKAVEIAGENAA
                     INDVQHAVRTINKNLAAIDKPRNLIIANLTSNLLLTHRQHLTQLLMPAGYMILSGIIE
                     QDAGKVAQEYAATPLELQRTITEKEWVCYVLKKTSAG"
     gene            1747..2850
                     /locus_tag="PLF77_07950"
     CDS             1747..2850
                     /locus_tag="PLF77_07950"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPC08825.1"
                     /translation="MKMKIAVLTGGGDCPGLNGAIKWVTKSALDPRLAAKRSVSFDVV
                     GIVEGWKGLIDVDPEDEKSRARHLKHLDEEIVRTWDRYGGTNLGTSRTNPFNPKNDRS
                     ARVIENIKKLGIDTIVAIGGEDTLGVAYKLHKMGIKTVGIPKTIDMDLAGTEYSLGFD
                     TAINIITEEIDRLRTTAGSHGRIFVVETMGRHAGWLALRGGECSGAYIILIPEHPFDM
                     DKVCSLLRERKGREINYSIIVVAEGAKLAGKQEFLKDSHIDDFGHVSVGGAGSFVAAE
                     IEKRTGLEARHISLSHLQRGGTPSAHDRMMARWFGIAAVDMIVKEDFGRMVTLERGDI
                     GTAPLAGVVNKLKLVDVEKYYDTERYNGRRTIF"
     assembly_gap    3139..3238
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3659..4819
                     /locus_tag="PLF77_07955"
     CDS             3659..4819
                     /locus_tag="PLF77_07955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPC08826.1"
                     /translation="MNINGISRKFSKLAYSLSELAENFSLIPNNDPKQALRIKRFFIS
                     IFVYLLNMPLSYLAYTGGLMEFKELVWGWSITTILMIILYIVFRSGLNKRMKDPSLTF
                     AQIFVASLVVMYGLFFVPEAKGILFAVYVLILLFGTFRLDTRQFFKASAFILGTYAVD
                     ICLLQIYRPEAIHLKLEVLYWFALGVVLASVSFIGGNISALRRDLAVSHKQLQASVEK
                     IQAMAIHDDLTGFYNRRHLMELLESEHNRSVRTGAVFSLAMIDLDKFKNINDKFGHQA
                     GDDVLMTFSNVIRNVLRKTDFCGRYGGEEFLIVLTQTDLEEARIFAERIRACIEDCLV
                     FDLEPDRQLKVTVSIGLAEHLKNEGIDRTIFRADEAMYKAKKNGRNRVEYSE"
     gene            5103..6779
                     /locus_tag="PLF77_07960"
     CDS             5103..6779
                     /locus_tag="PLF77_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08827.1"
                     /translation="MKKIVVFSAVCVLVFFMAVSGMAAVWPPAGFTESVADQNTVFPQ
                     TKATAVTVFAPLPASCPSHPAACDKLNFLRIRHILGPDNPSNADRILIAQPGILEGAS
                     AFYNVAANLVTRAYDEKGKFVEFWAIDRRSNCLEDMNGLRLARETGNLHDMVDYYYRN
                     KPYHGQYFQGYFKPLSDDVKWLVEMGMEQTLKDWNEVITRGIPDPTVRQQKVYIGGHS
                     LGGFLTGAFACADFDGDLTTLADAGYNQAAGYFALDSLITSDPLLSVQGGDSATLRYL
                     MGKIPDSAVYLMRKGLFERFIFLSGVITPEIMNLLTGVGYAAMVAPNAETDLISYLPA
                     STSVKNCYRFYHSKNIWDYALVTPSIYKFRYTNQALMALFMDDNAMPINIIQASMGFF
                     AGGTVAEKQFPFGSGGTLAIANNSGTALKKGPLYRWINYDEIEGSAIPNNKDGLPFTT
                     AAKEVTDAKDLARSLGALPMDFVEKYFPMRLAVESMMGSDVIVHPDGISQRPVINIVA
                     GDGAKLGLDQLSPDSPIIPGYNHLDVLTAAALQNDGEPEKVTTHLLDFIF"
     gene            6937..7578
                     /locus_tag="PLF77_07965"
     CDS             6937..7578
                     /locus_tag="PLF77_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HPC08828.1"
                     /translation="MKYYPVFLDIKDKICVIVGGGEVAFRKAERLLECGAKVSIISPK
                     LVRELAALKEKNLISHIAAEYSGDLIDRAVLIIGATDDEKINALIAQDARAKGIPVNI
                     VDDPQKCDFILPAIAQRGDLVMAIGTGGKSPALARHLREELEEQYGREYAILLNILGT
                     LRTKMEKNAGIGRDWFDALMAAGILEFIKAKDSKKVQDIVKKITGEEVECEFR"
     gene            7565..8395
                     /gene="ccsA"
                     /locus_tag="PLF77_07970"
     CDS             7565..8395
                     /gene="ccsA"
                     /locus_tag="PLF77_07970"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPC08829.1"
                     /translation="MSFVNLEMLSFNIALVLCGASTIGYLSSLLVKKVKLAKMSTWVL
                     AAAFAFLTVNFFFVMVNSSWFVNPDSKDFLSLCAWAVCAVYLVLQFKTKTRVLGAFIS
                     PLILLFLIAAAGEEAGKSIRPQNLQSGLTAVHLFLTIFGEVLFVIASCAGLMFIMQNR
                     LLKSKKLSGMSRLLPSLNDLDRINHMCLLWGFPILSVGIIAGVIFAELSWETGWLTDP
                     KIIWTFAAWMIYGFLLHQRLAIGWKGYRMALISGVTFILLIVSYVGVKICCPTLHNFI
                     "
     gene            8398..9657
                     /gene="hemA"
                     /locus_tag="PLF77_07975"
     CDS             8398..9657
                     /gene="hemA"
                     /locus_tag="PLF77_07975"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HPC08830.1"
                     /translation="MIVLTGLNHKTAPLAVREKIFAGCQEKKDLLPALRSIGGVEEVL
                     YLSTCNRVEILASVAEDGNALKELSGFLARNGGLNQAEAANSFYEYRGEDAVRHIFRV
                     ASSLDSMVMGEAQILGQVKDAYREALAKYATGVVINRLMHCSFRAAKRVRSETGIAVN
                     PVSVSHAAVELAKKIFGTLTGKIILLIGAGEMAELTGTQLIEKGAQSIIVANRSLAQA
                     ELLAEKFHGEAVALDALEKKLIEADIVISSTGAPDFIVTAGMLKKIHHRRKNRLLFLI
                     DIAVPRDIDPDASELENVYLYNIDNLQDIVDENINARRKEAIKAEKIIDEELERFINW
                     QKELESVPTIVSLRNKADDIVKAEIEKASGWMNDLKKEDQEKIEILINSIVNKVLHSP
                     VSVMKEESSEFSSRDIVAAVRRLFRLD"
     gene            9692..10609
                     /gene="hemC"
                     /locus_tag="PLF77_07980"
     CDS             9692..10609
                     /gene="hemC"
                     /locus_tag="PLF77_07980"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HPC08831.1"
                     /translation="MTNIKVGTRGSKLALMQTNFVAEKLKKIMPNANIEICVIKTSGD
                     IMQDVSLLTIGGQGVFVKEIEEALLSEKIDLAVHSLKDVPGENPEGLIFAAILPREDV
                     RDVLVSRNNIKLEFMPKGAKIGTGSQRRGAQIKALLPDVSIVPLRGNIETRIKKIETE
                     NINGVILAAAGMKRNGLAEKITQFLPVETMLPAVGQGALGLQIRKDDTELAKACAALN
                     DTTTAAEVAAERAFLRALGGGCRLPIAALGKLDGRMLTLEGMVAAPNGSTMIREKLSG
                     TINDVEAMGKKLAEIVLDKGGRRLMDLMC"
     gene            10706..12259
                     /gene="cobA"
                     /locus_tag="PLF77_07985"
     CDS             10706..12259
                     /gene="cobA"
                     /locus_tag="PLF77_07985"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HPC08832.1"
                     /translation="MMKKNKKGKVYIVGAGPGDAGLMTLKGIDCLREADVVIYDYLVS
                     KDLLKYAKSDARFIYVGKQGGAHTLSQWQINDLLVKEAKAGNVVARLKGGDPFIFGRG
                     GEEAEKLVANKISFEIVPGVTSAIAVPAYAGIPLTHRGLTSTVAFVTGHEDPTKEKSD
                     INWSALSRIGTLVFLMGMKNIEKIVRELKDNGRSPKTPAALIRWGTTPRQKILEGTLA
                     DIVNLAKDRKFVPPAILIVGEVVALRDRLQWFDSKPLFGKGVVITRPEKQADDLAQLL
                     IKEGATPIHFPTIKIVPPLSWRELDKAIKKLDDYDWLIFTSANGVAYFFERLSAKGKD
                     IRDLKGIKICCIGPATARQVQDKGIRVDLIPGEFISEGILKSFARKKLKGKKILLARA
                     AEARDVLPEGLKKLGARVDVATAYVTVTSGRKKNELEDLFNENQVDVITFTSSSTVNN
                     FRKIMGSGFRLPEGVKIASIGPVTAATARKAGFPVDIHQEEYTMEGLVNALNAYFGKK
                     SSGKRGKNK"
BASE COUNT         3080 a         2792 c         3240 g         3095 t
ORIGIN      
        1 ttccgggatt cttcttccca gaaaatcagt ttcaaagcct tgggagaaca ccgggaaagc
       61 atgtccggaa aagataaaat ttttgtgacc tccgtgatgt aaacgctgtg actcgcccgg
      121 gcggcttcgt gtgctatttt ctgccagcgg gtctgtttca ggggattctt ttcctcggca
      181 atatggctga ccgaccgcac ggcatgaaaa ggaataatca tgtccgctcc cagttcggcg
      241 gcgcttttaa cgatcgtgtc catttttttc cctttgggga tggcctgcgc cagggtgatg
      301 ctgatgtccc tgttaacgga gggaatcgct ttccccaagt cgatgagcac cgattttgcc
      361 gaaaaatttt tgatggtcgc ttcgtattcc cgcccgaagc cgtcaaaaat gtttatccgg
      421 tcgccctctt tgaggcgcat gacctttttc agatatttta cattgtcttc tcccagcgcg
      481 cagctgtttt tattttccag agattccgga ctgtatatgc gggggacggt caaggcctgt
      541 tttcctttat agagggttta tctgaaaatt gccgtttgca gggtgctgtc agccggctga
      601 tgtcttttta agcacgtaac agacccactc cttttccgtg attgtccgtt gcagttcaag
      661 aggtgttgcg gcatattcct gcgcgacttt tccggcatct tgttcaatga tgccggaaag
      721 gatcatgtag ccggccggca tcagcaattg cgtcagatgc tgccggtggg tcagcagtag
      781 attcgacgtg aggttggcga taatcagatt gcggggtttg tcaatggcag ccagattttt
      841 attgatggtg cggacggcgt gctgcacatc attaatggcc gcgttttcac ccgcaatttc
      901 gacggctttc cggtcgatat ccgcacagag aacatcctgc gctcccattt tggcggcggt
      961 aatgccgagg atgccggtgc cgcaaccgac atccagcact ttccattctt taatggaacg
     1021 gtcgttcatg atgatatctt caaccgcttc aatgcacatt ctggtggacg catgctggcc
     1081 ggtgccgaat gccatgccgg gatcgatttc aataacaatg tctcgactgg aaggcgtgta
     1141 tcgttcccat gtcggtttga caacgatgtt gttgctgacc cggatgggtt taaaatattt
     1201 tttccattgt tctccccaat cgggatcgca gatggtttcg gttgtaaatg acggctttcc
     1261 cagatcgggg aatatttcgg ccaggctgtc gatgtatttt tgcagtgccg ccagtttttt
     1321 atcgctgcgg ttgtcgagcg ggaaataggc tttcaggatg tcttccgcaa ccggttcggg
     1381 aaagtctgaa ccgacggcct gagaggactg cgattcctga aacacgccct gaacaccgga
     1441 ttccgtgaga aaatttccca gcgcctccat cagttccggg ggagccaata tttcgatttt
     1501 cagccattta ccggtttctt tttctgtcat gaaagcccgt cctgtttttt gtaaaatata
     1561 tattttttta agtaatattt caagcgtcct gacgaaaaga tgaaaagaat atcctccgtc
     1621 caaggggaat ataaggttga cagaagaggc ggtttttctt ataaaatgaa catcgcgatt
     1681 tttacatcag gatccgtcaa ttattaacga gtgttttatt aactttcaat attgcggaga
     1741 aaaaatatga agatgaaaat agcagtactg accggcggcg gagattgtcc cggtttgaac
     1801 ggggcaatta aatgggttac aaaaagtgcg ctcgatccgc ggctggcggc caagcggtcg
     1861 gtttcctttg acgttgtcgg tatcgtggaa ggctggaaag ggcttatcga cgttgatccg
     1921 gaggatgaaa aaagccgcgc ccgtcacctg aaacatttgg acgaagagat cgtccggaca
     1981 tgggatcgct acggcggaac gaatctgggg acatcgcgga ccaatccctt taatccgaaa
     2041 aatgaccggt ccgcccgggt cattgagaat attaaaaagc tcggcatcga caccatcgtc
     2101 gcaattggcg gggaagacac gctgggtgtt gcgtataaac tacacaaaat gggcatcaaa
     2161 acggtgggca ttcccaaaac catcgatatg gatctggccg ggacggaata ttcgctcggt
     2221 tttgacacgg cgattaacat tatcacggag gaaattgacc gtctccggac aacggcggga
     2281 tcccacgggc gcattttcgt ggtggaaacc atgggacggc atgccggttg gcttgcgctg
     2341 cgcggcggcg aatgcagcgg cgcctacatc attctgattc cggaacatcc gttcgatatg
     2401 gataaagtct gctcgctgtt gcgcgaaaga aaaggccggg aaataaatta ttccatcatt
     2461 gtggtggccg aaggcgccaa acttgcgggg aaacaggaat ttttaaaaga ttctcatatt
     2521 gatgatttcg gtcatgtctc cgtcggcgga gcgggcagtt ttgttgccgc agaaattgaa
     2581 aagaggaccg gactggaagc ccgtcatatc agcctgagcc atcttcagcg cggaggaacg
     2641 ccttcagcgc atgaccgtat gatggcccgg tggtttggca ttgcggcggt ggacatgatt
     2701 gtcaaggaag atttcggacg catggttacc ctggaacgcg gcgatatcgg cacagcgcct
     2761 ttggccggag tggtcaataa actgaaactt gttgacgtgg aaaaatacta cgacaccgaa
     2821 agatacaacg gaagacgaac tatattttag tgagccaagc tgaaaacgag cgcagcgaag
     2881 tttgaagcgt aaggcgaact atcccctgaa gcgaggcggc gcgggatgtg agccaagctg
     2941 aaaacgagcg cagcgaagtt tgaagcgtaa ggcgaactat cccctgaagc gaggcggcgc
     3001 gggatgtgag ccaagctgaa aacgagcgca gcgaagtttg aagcgtaagg cgaactatcc
     3061 cctgaagcga ggcggcgcgg gatgtgagcc aagctgaaaa cgagcgcagc gaagtttgaa
     3121 gcgtaaggcg aactatccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnga
     3241 gccaagctga aaacgagcgc agcgaagttt gaagcgtaag gcgaactatc ccctgaagcg
     3301 aggcgaatgg ggattcgaag gcagcaccgt gggattcgag gtaaatcact gaagcttgct
     3361 ggccgtaatg agactcaaat ttcagaaaca tagtgtgatg aaatttgtta gtcgaattat
     3421 ccaactccga ggtatcggaa ttaggaccca ttcgcggatc gcaaccggat aaaatgaaat
     3481 cgcggcattg catccgcaac aaaatcattg ctgatttacg gcttcgtcct tttaaaaatt
     3541 atattaagtt tgtgtggcgt tcccgcttgt tttgtgctta aggaaacgca tagtcgccta
     3601 ttcttctaat ctttttgaaa aacagctttt tgcatatatg gcttataaaa ggatatcctt
     3661 gaatataaac ggtatttcga gaaagttttc caaactggcc tattctcttt ccgaattagc
     3721 ggaaaacttt tccctcattc ctaataacga tcccaagcag gctttgcgta ttaaacgttt
     3781 tttcatttca atatttgtct atcttttgaa catgccgctt tcttatctgg catacaccgg
     3841 cggcctgatg gagtttaaag agctcgtatg gggttggagt atcacgacga ttttgatgat
     3901 tattttgtat atcgtttttc gcagcggcct taataaaaga atgaaggatc ccagcctgac
     3961 ctttgcccaa atattcgtcg ccagtctggt tgtcatgtat ggcctgtttt ttgttccgga
     4021 agccaagggg attctttttg cggtgtatgt tcttattctg ttgttcggaa cctttcgcct
     4081 ggacacacgt cagtttttta aagccagtgc gtttattctg gggacatatg cggtggatat
     4141 ctgtctgctt cagatttacc ggccggaggc tattcatctc aaattggagg ttctttactg
     4201 gttcgccctg ggtgttgttc ttgcttccgt ctctttcatc ggcggcaaca tcagcgcttt
     4261 gcgccgtgat ctggctgtga gccacaagca gcttcaggcc tctgtggaaa aaatccaggc
     4321 catggcgata catgatgacc tgacggggtt ttataatcgt cgtcatctga tggagcttct
     4381 tgaatccgag cataaccgct cggtccgtac cggcgcggtt ttttctctgg cgatgattga
     4441 tcttgataaa ttcaaaaata ttaatgacaa gttcggacac caggccggag atgatgtttt
     4501 gatgacgttt tccaatgtga tccgcaatgt tttgcgaaaa accgatttct gcggccgcta
     4561 cgggggagag gaatttttga ttgttctgac gcaaacagat ctggaagagg ccagaatttt
     4621 tgccgaacgt atccgtgcct gcattgaaga ttgtctcgtt tttgatctgg agcccgaccg
     4681 tcaattgaag gtgacggttt ccatcggttt ggctgagcat ttgaaaaatg aaggtattga
     4741 ccggaccatt ttccgcgccg atgaggccat gtacaaggct aaaaagaacg gacgcaaccg
     4801 cgtggagtat tcggaatagc agtaggcccg ccgtcttcaa ctgcgatcca ggataatcct
     4861 taaattattt tcagaaaata tctgtgttcg tccgactaat tttttttcgt ctgttttccc
     4921 gcttgacaaa cgttttcaat ttattttaag aagaaaacaa attgtacgtt cttcatctca
     4981 cactgtacgt ttcttccccc ggacaaatcc atataatata aattttttac attaaaataa
     5041 gtatagttag ctgtgcagga ttttcttcgt tcttaattct aaatcagggg aggaatgggt
     5101 ttatgaaaaa aatagttgta ttttcagctg tttgtgtatt ggtttttttt atggctgtaa
     5161 gcggcatggc tgccgtctgg cctccggcag gcttcacgga aagcgtggcg gatcaaaaca
     5221 ccgtgtttcc gcaaacgaag gcaacagcgg tgactgtttt tgcacccttg ccggcttcgt
     5281 gtccatccca cccggcagcc tgcgacaaac tcaattttct cagaatcagg catattctcg
     5341 gaccggacaa tccctccaac gcggacagaa ttctgatcgc tcaaccgggt attctggaag
     5401 gcgcatcggc attctacaat gtcgcggcca atctggtgac gcgagcctac gatgagaaag
     5461 gcaagtttgt ggaattttgg gcgattgaca gacgatcaaa ttgtctcgaa gacatgaacg
     5521 gcctcaggct ggcaagggaa accggcaatc tgcacgacat ggtcgattat tattaccgga
     5581 acaaaccgta ccatgggcag tatttccagg ggtattttaa gcccctgagc gacgacgtga
     5641 aatggctcgt ggaaatgggc atggaacaga ccctgaagga ctggaacgag gtcatcacgc
     5701 gcggcattcc cgatccgacg gttcgtcagc agaaagtcta catcggcggt cactctcttg
     5761 gcggattcct caccggcgct tttgcctgcg ccgattttga cggcgatctg acaacacttg
     5821 ctgacgcggg atacaatcag gctgccggct atttcgctct ggattccctg attacatccg
     5881 atccgttgct ttccgtgcag ggaggggact cggccactct gcgctatctg atgggcaaga
     5941 taccggacag cgctgtctat ttaatgcgga agggtttgtt tgaacgattt atcttccttt
     6001 ccggcgtcat tactcctgag atcatgaacc tgctgaccgg tgtcggctat gctgccatgg
     6061 tcgcaccgaa tgcagaaaca gatttaatat cgtatttgcc ggcctcaacc agtgtcaaaa
     6121 actgctaccg gttttaccat tcaaaaaata tatgggatta cgcattggtc actccttcca
     6181 tctacaagtt ccggtacacc aatcaggctt tgatggccct gtttatggat gacaacgcaa
     6241 tgccgattaa tatcattcag gcaagcatgg gtttttttgc ggggggaacg gttgcagaga
     6301 aacagtttcc cttcggaagc gggggaacgc tcgccattgc aaacaacagc ggcacggctc
     6361 tgaagaaggg accgctctat cgatggatca attacgatga aatcgaaggg tctgcgattc
     6421 cgaacaacaa agacggcctg ccttttacga ctgccgccaa ggaagtcacc gatgcaaagg
     6481 atctggcgcg gtctttaggc gcgctgccga tggattttgt ggaaaaatat tttccgatgc
     6541 ggctggcggt cgagtccatg atgggcagcg atgtgatcgt gcatccggac ggtatttccc
     6601 aacggcccgt tattaatatt gttgcgggag acggcgcgaa gctcggcttg gaccagttgt
     6661 cgccggattc gcccattatc cccggatata atcatctgga cgtcctgacg gctgcagctt
     6721 tacaaaatga cggcgaaccg gaaaaagtga ccacacatct gctcgatttc atcttttaac
     6781 agtttctttg tagagaaaac ctgaacaaat cgcggcgtcc gtaaatggac gccgcgatgc
     6841 tttcatctta tacgttcttg aattttaccc cccttcgtga tagctattcc gaaaatgctt
     6901 ttggccgcgg cccgtcgggc gaaaggacaa aaactattga aatattaccc tgttttttta
     6961 gacatcaagg acaaaatttg cgtcatcgtc ggcggcggcg aagtcgcctt cagaaaagct
     7021 gaaagacttc tggagtgtgg cgcgaaagtc tccattatca gtccgaaact tgtccgggaa
     7081 cttgccgcgc tcaaagagaa aaatctgatt tcccacattg ccgccgaata ttccggcgat
     7141 ttgattgacc gggccgtcct gattatcggg gcgaccgatg acgaaaagat caacgctctc
     7201 atagcgcagg atgcgcgggc caagggcata cctgtaaata ttgtcgacga tccgcaaaaa
     7261 tgcgatttta ttctgcccgc catcgcccag agaggcgatc tggtcatggc tatcggcacc
     7321 ggcggtaaaa gcccggccct ggcgcgtcat ttacgggaag agcttgaaga acaatacggc
     7381 agggaatacg caatattgct gaatatttta ggaaccctgc gcaccaagat ggaaaaaaac
     7441 gccggaatcg gcagagactg gtttgatgcc ttgatggcgg caggcatcct ggaattcatc
     7501 aaagccaaag acagcaagaa agtgcaggat atcgtcaaga aaataaccgg tgaggaggtg
     7561 gaatgtgagt ttcgttaatc ttgaaatgtt atcgtttaac attgcccttg tcctctgcgg
     7621 cgcaagcacc atcggttatc tgtcttcgct tcttgtcaaa aaagtaaaac tggctaaaat
     7681 gtccacttgg gttcttgccg ctgcttttgc atttctgacc gttaatttct tttttgtcat
     7741 ggttaattct tcttggtttg taaatcccga ctccaaggat tttctttccc tttgtgcctg
     7801 ggcggtgtgc gcagtttatc tggtattgca atttaaaacg aaaaccagag tgctcggggc
     7861 ttttatttcg ccgttgatcc tgcttttttt gattgcggcc gccggagagg aagcgggcaa
     7921 atctattcgt ccgcagaatc tgcaaagcgg gctgaccgcc gtgcatttat ttttgaccat
     7981 tttcggggaa gtgctttttg tcatcgcttc ctgcgccgga ttgatgttca tcatgcaaaa
     8041 tcgtctgctg aagagcaaga agttaagcgg catgagcaga cttctgcctt ctttaaacga
     8101 tctcgataga atcaaccata tgtgtctttt atggggattc ccgattttga gtgtgggcat
     8161 tattgccgga gtgattttcg cggagttaag ctgggaaacc ggctggctga ccgatccgaa
     8221 aatcatctgg acgtttgccg cctggatgat ttacggtttt ttgctgcatc agcgtctggc
     8281 tatcggctgg aagggttacc ggatggctct catttccggg gtcaccttta ttcttcttat
     8341 cgtgtcttac gttggcgtga aaatttgttg tccgacgttg cataacttta tttaaatatg
     8401 attgtactga ctggcctgaa tcataaaaca gcccccctag ctgtccggga aaaaattttc
     8461 gccggctgtc aggagaaaaa agatttgctc ccggcgctgc gttccatcgg cggcgtggag
     8521 gaggtgctgt atctttccac ctgcaatcgg gtggagattc tcgccagtgt tgcagaagac
     8581 ggtaacgctc tcaaagaact cagcggattc ctggcacgaa acggcggttt gaaccaggcc
     8641 gaagctgcca atagctttta tgaatatcga ggcgaagacg cggtgcgcca cattttccga
     8701 gtggcgtcta gccttgattc gatggtcatg ggcgaagcgc agattctcgg gcaggtgaag
     8761 gatgcctatc gcgaagcgct ggccaaatac gctaccggcg tcgttataaa ccgtctgatg
     8821 cactgctctt tccgcgcggc caaacgcgtc cgttccgaaa ccggcattgc cgtcaatccc
     8881 gtttcggtga gccacgccgc ggtggagctg gccaagaaaa tctttggaac gcttaccggc
     8941 aaaataatcc tgctgatcgg cgccggagaa atggcggagc tcaccggcac acaactcatt
     9001 gaaaaaggtg ctcagtcgat tatcgtggcc aaccggtcgc tggcacaggc cgaactgctg
     9061 gcggaaaaat tccacggcga agcggtggcg ctcgatgcct tggagaagaa gctcattgag
     9121 gctgatatcg tcatcagttc cacgggcgct cctgatttta tcgttaccgc cggtatgctt
     9181 aaaaaaattc atcaccggcg taaaaaccgc ctgctgtttc tgattgatat tgccgttccg
     9241 cgcgacattg atccggacgc cagtgagctg gagaatgttt atctgtacaa catcgacaac
     9301 cttcaggaca tcgttgacga aaacatcaac gcccgccgga aagaggcgat caaggcggaa
     9361 aaaattatag acgaggaatt ggagaggttc atcaactggc agaaggaact cgaatccgtg
     9421 ccgacaatcg tctccctgcg caacaaggca gatgatatcg tcaaggccga aatagaaaaa
     9481 gcttccggct ggatgaatga tttgaaaaaa gaagatcagg agaaaatcga gattctgatc
     9541 aactccatcg tgaacaaggt gctgcattct ccggtatcgg tgatgaagga agaaagctcc
     9601 gaattcagct cccgcgacat cgtcgcggcg gtgagaagat tgtttcggtt ggattgatga
     9661 aaagcagtaa aacgtaaagg gtgaatggtg aatgacaaat attaaagtag gtacacgcgg
     9721 cagcaagctt gctctgatgc aaaccaattt tgttgctgag aaattaaaaa aaataatgcc
     9781 gaacgcgaac attgaaattt gtgttatcaa aaccagcggc gacattatgc aggatgtgtc
     9841 gctgctgacc atcggaggcc agggcgtttt tgtcaaggaa attgaagagg ctctgctttc
     9901 ggaaaaaatc gatctggcgg tacacagcct gaaagatgtg ccgggagaaa atccggaagg
     9961 attgatattc gcggcgattc tgccgcgcga agatgtgcgc gatgttctgg tctcgcgaaa
    10021 caacatcaaa ttggaattca tgccgaaagg agcaaaaatc ggcaccggat cgcagcggcg
    10081 cggcgcgcag atcaaagcgc tgctgcctga tgtgagtatt gtgccgctca ggggcaatat
    10141 cgaaaccaga attaaaaaaa ttgaaacgga aaatatcaac ggtgtaattt tagcggcagc
    10201 ggggatgaaa aggaacggac tggctgaaaa gataacgcag tttctgccgg tggaaacgat
    10261 gctcccggca gtcggtcagg gagcgttggg tttgcagatt cgcaaggacg ataccgaact
    10321 ggcgaaggcc tgcgccgcat tgaatgacac aacaacggcg gcggaagttg ccgccgaacg
    10381 cgcgtttctg cgggcgctgg gcggtggatg ccgcctgccg atcgcagcac tgggaaaact
    10441 ggacggccgg atgctgacgc tcgaaggcat ggtcgcggca ccgaacggtt ccacgatgat
    10501 tcgtgaaaaa ttaagcggta cgataaatga cgtggaagca atgggcaaga aactggcgga
    10561 aatcgttctg gacaagggcg gccgaagact gatggatttg atgtgctgat attaatatgt
    10621 catttcgaag cccagcaaga aatcttgttt ttataagatt ggttttttaa gattcctcac
    10681 atacgttcgg aatgacaaat ggacaatgat gaaaaagaat aaaaaaggaa aagtttatat
    10741 cgttggcgcc ggaccgggcg atgcagggct gatgacgctc aagggtattg attgcctgcg
    10801 ggaagcggat gtcgtcattt acgattatct ggtcagcaag gatttgttaa aatacgcaaa
    10861 aagcgatgcc cgctttattt acgtcggcaa acagggcggc gcccataccc tgtcgcaatg
    10921 gcagatcaac gatctgctgg tgaaggaagc taaagcgggt aatgttgtcg cacgcctcaa
    10981 aggcggcgat ccttttatct tcggccgcgg cggagaagag gcggaaaagc tagtcgccaa
    11041 caaaatatcc tttgaaatag ttcccggcgt cacctcggcg attgccgttc ccgcttatgc
    11101 cggaataccg ctgacgcacc gaggtctgac atccaccgta gcgtttgtta ccggccacga
    11161 agacccgacc aaagaaaaaa gcgatatcaa ctggtctgct ctgtcccgca tcgggacgct
    11221 ggtgtttctt atgggcatga agaatatcga aaaaatcgtc cgggaattga aagacaacgg
    11281 ccgatcaccc aaaaccccgg ccgcgctgat tcgctggggc acaacgccca ggcagaaaat
    11341 tctcgagggc actctggccg atatcgtcaa tctggcgaaa gatcgaaaat ttgtaccccc
    11401 ggcgattctt atcgtgggcg aggtggttgc tctgcgcgat agattacagt ggtttgattc
    11461 aaagccgctt ttcggcaaag gtgttgtgat tacgaggccc gaaaagcagg ccgatgatct
    11521 ggcgcaactg ctgatcaaag aaggagcaac cccgattcat tttccgacca tcaagattgt
    11581 tcctccgctt tcctggcgcg aactggataa agccataaaa aaactggatg attacgactg
    11641 gctgatattc accagcgcga atggtgtcgc ctatttcttt gaaaggcttt cggccaaagg
    11701 caaagacatc cgcgacttga agggtatcaa aatttgttgt atcggtccgg ctacagccag
    11761 acaggttcaa gacaagggaa tccgggttga tcttattccc ggagaattta tttcggaagg
    11821 aattttgaag tcctttgccc gcaaaaaact gaaaggaaag aaaatacttc ttgccagagc
    11881 tgcagaggcg cgcgatgttc ttcccgaagg actcaaaaag cttggcgcgc gcgttgatgt
    11941 cgcgacagcc tacgtgacgg taacctcggg aagaaagaaa aatgaactgg aagatctctt
    12001 taatgaaaat caggtggatg tgattacctt tacaagttct tcaacagtga ataactttag
    12061 gaaaataatg ggaagcggtt ttcggctgcc cgaaggagtg aaaattgcca gtatcgggcc
    12121 ggtgaccgct gcaaccgcca ggaaagcagg attccccgtt gatattcatc aggaagaata
    12181 taccatggag ggactggtga acgccttaaa tgcgtacttc ggaaaaaaat cttcaggcaa
    12241 aagaggcaaa aataaatgat aggaatctcc aaattatatt gcggggctgt ggaaccgtcc
    12301 gatgttt
//
LOCUS       DAOOLB010000061        12170 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_5793_length_12170_cov_47.266777, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000061 DAOOLB010000000
VERSION     DAOOLB010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 12170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12170
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5793_length_12170_cov_47.266777"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1414
                     /locus_tag="PLF77_07990"
     CDS             <1..1414
                     /locus_tag="PLF77_07990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC08833.1"
                     /translation="GIETMNDITMERRHEDRRHDDRRRGERRKYERREDVRKMNQELI
                     REQMEKYRVLLKDIKDAYFEVDLLGNFTVVNSAMCSALGYGPEELIGSKYRKLVDKDG
                     AKKLQQAVERLHKNDEPVNLLDMEAAKKDGTKIIYEAMVSVIRETDGVVVGFRGVGRD
                     VTRRRQMEAELKRNQHDLIRKNQEIDQSRKDIQQALVSLEKAHEELKASQLKILQQEK
                     MASIGQLAAGVAHELNNPMSFISSNLSTLEKYINRLIQFIRVQSEAVASLQDAAVVRR
                     VDEKRAEVKLDYMVEDVRGLLKESLEGAQRVKKIVYELNSFSRMDEEEYKKADIHECI
                     ESAITIVWNELKYKSKLEKHYGDLPATMCYPRQMNQVFVNLLINAVNAIVDKGTITIK
                     TWYEDHAIRIDVSDTGAGIPRENLIRIFEPFFTTKAVGKGTGLGLSITYEIIQRHRGE
                     ITVASEEGRGTTFSIKIPVV"
     gene            1655..2797
                     /locus_tag="PLF77_07995"
     CDS             1655..2797
                     /locus_tag="PLF77_07995"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPC08834.1"
                     /translation="MSKNIFRIVFVLIFSFLTAGVVLAAPPVKIGALFAVTGPASPLG
                     EPERNTAVMMVDEINKAGGIKGRKLELIVYDTQGDATKAVQAVNKLIKDDKVVAIIGP
                     STTGDSMAVIPVIEKSQVPLISCAAGIKITDPVKKWVFKTAQNDVLAVMRIYKNLQKQ
                     KISKVAILTVSDGFGSSGREQLRLQAKEFDISIISDETYGPKDTDMTVQLTKIRNSAA
                     QAVICWGTNPGPAIVARNARQLGIKIPLYMSHGVSSKKFIELAGEAAEGIMLPSGKVI
                     VADQLPASDPQKKTLLNYVSKYKERYKVEGDHFGGHAYDAVMLLKRAMEQGGFTPAAI
                     RDQLEKTKKFAGIGGTFSFSSRDHAGLTSDAFVLVRVEKGDWKLIN"
     gene            3007..3891
                     /locus_tag="PLF77_08000"
     CDS             3007..3891
                     /locus_tag="PLF77_08000"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPC08835.1"
                     /translation="MDNFQQLFQYVLTGLSNGAIYALIGFGFAVIYNSTGIINFAQGE
                     FVMLGGMLTVFLLTVLHVSLVPAIALAIVVSTLVGILFERFAIRPLKDAQPLSLIIIT
                     IGASIFIRGVAMLVWGKDTHALPAFSSHDPLSIAGATILPQHLWILGVTILIIAASRL
                     FFNHSIIGKAMRACSYNPRAAGLVGINVKSMVLLSFSLSAAIGSLAGIIIAPLTMTSF
                     DVGVMLGLKGFCAVIIGGMSSGMGTVLGGLLLGLLEALGAGFISASYKDAIAFIILLL
                     ILFIRPEGLFRKKETERF"
     gene            3941..4912
                     /locus_tag="PLF77_08005"
     CDS             3941..4912
                     /locus_tag="PLF77_08005"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HPC08836.1"
                     /translation="MLKEKKHQIFIFVIFALAVLSVPFFLQGNYLLNVLVFVGINTML
                     AVGLNLLLGYAGQISLGHAAFFGMGAYISGIVTSRYPVEPFLIIFLAAVCVGLIAFVI
                     GFPILKLKGHYLAMATLGFGIIMYIIFNETVDWTGGPSGLSGIPSLKIGPVVFDNDQS
                     NYYLVWVFTLVVMMLSINLSNSRIGRALRAIHDSEVAARVMGVNARFLKVQIFTVSAV
                     ISAVAGSLYAHVMTFIAPASFGFNFSVELLTMIVIGGLGSVYGSFLGAVILTMLPEFL
                     RVFQDLDIVIYGLMLILMTMYMPGGLISGIEMAAEFFSSRLRKGKGE"
     gene            4909..5664
                     /locus_tag="PLF77_08010"
     CDS             4909..5664
                     /locus_tag="PLF77_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC08837.1"
                     /translation="MMLRLSNITQIFGGLNALEDVSFSVREGSITGIIGPNGAGKTTL
                     FNIVTGLYTQTGGDVFLGGENISFLETEELAGRGLVRTFQNVELFRKMTVLENVMVGL
                     HTKSSSGIFSCAFKLPRQIREEKAIREKSMVWLKYTGIAELAEMTAGNLPFGKGRLLE
                     ISRALAAGPKIILMDEPAAGLNSRETAELALLIRKIRQSGVTVVLVEHDMDLVMDVCD
                     AIVVLNLGKKLAEGTPREIQENPAVISAYLGEG"
     gene            5778..6521
                     /locus_tag="PLF77_08015"
     CDS             5778..6521
                     /locus_tag="PLF77_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC08838.1"
                     /translation="MLKIKNINTYYGQAQALKNVSLHLAEGEIVTLIGANGAGKTTLL
                     NSLSGVVPTRSGQIIFNDAPINNLPVHQIVRLGISQVPEGRQVFKPVSVEDNLELGFY
                     LHHRIFGGARDVKKNKEMVYDLFPILRERRKQLAGTLSGGEQQMLAIGRAFMTRPKLM
                     LLDEPSMGLAPMVVQEIFRVIASLSREQKTTVLLVEQNARAALKMAGRGYVLETGHMI
                     LEGTAAELLNNKDVQRAYLGRDKKEIWER"
     gene            6601..7902
                     /locus_tag="PLF77_08020"
     CDS             6601..7902
                     /locus_tag="PLF77_08020"
                     /inference="COORDINATES: protein motif:HMM:NF025888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPC08839.1"
                     /translation="MNIWDQTHECMARDELEQLQLERLQAVVNRVYKNVTHYRKIFNE
                     TGIIAEDIRSLADLSRLPFTTRDDLQVNYPYGMFAVPLREVVRIHSSSGTTSKPVVMG
                     YTKNDIKIWSNLAARFMTAAGVTPDDVVQITFRYGLFTGAFGVHYGAEAIGASVIPMG
                     TGNTDKQIMIMQDYKSTVLVSTSSYAIALADRLDQLGIDPKSLSLKTGLFGGEPWSEK
                     MREEIENRLLLKATDNYVISELIGPGVAGECLCKKGMHIYEDAFIPEIIDPETGAVLP
                     AGAEGELVLTTLTREAFPMIRYRTGDMTSLDYAPCACGRTLVRMKKIMRRSDDMIIVR
                     GVNVFPAQIEDALFSVAQGETPYQIVVEREGAMDNLEVVVEVTDKIFSLELQKQRSFL
                     ETIKKRLASVTGINVVVRLVESKSIPRQQGRIQRVLDKRQI"
     gene            complement(7915..8979)
                     /gene="bioB"
                     /locus_tag="PLF77_08025"
     CDS             complement(7915..8979)
                     /gene="bioB"
                     /locus_tag="PLF77_08025"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HPC08840.1"
                     /translation="MNFVDHLKNKIIRQSRFITPAEAMELFIEASGFCWSVLAAASEI
                     REHFKGKKIIFCGITNAKSGRCPEDCMFCAQSSHYKTTAHSYPLKKSAQIIADAEKAV
                     RNGAECFGIVTSGKQLKSKKERLEIIRAIQGVRETGLFPCASLGMIDKATAMELKAAG
                     LFRYHHNLETSRSFFKTVCTTHDYEESVKTLCAAKEAGLSVCSGALIGMGENISHRIE
                     LAQTLRKLDVDSVPLNILHPVKGTPLSHMRALPPMEILMTLAVFRFMLPDRDIRLCGG
                     KEKNLRQLLPLGIVAGANALMTGNDLTIPGRDSSLDHEMIADLGLTATRERITCTCNI
                     EKHSRPETGPKRRPVQKHPS"
     gene            complement(8976..9680)
                     /locus_tag="PLF77_08030"
     CDS             complement(8976..9680)
                     /locus_tag="PLF77_08030"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HPC08841.1"
                     /translation="MDKRLAYRIFFLFLFIALSAIIFIHYDLHHLFRDRDRLVNFILS
                     YPYDQFIFILVQIIQVVAAPIPGELTGIIGGYLYGPLWGTLYSTIGLTIGSWIAFMLA
                     RFFGEPLLENVVKKEVFDKFDHFMEHKGLLVSFLLFLIPGFPKDYLCYIMGVSRIPVG
                     TFIIISTAGRIFGTIMLSITGNVARNGQYILLTVILILGVTVFIAVYYYHDQLINILK
                     KEGAKLGHVKKERKSK"
     gene            9923..11281
                     /locus_tag="PLF77_08035"
     CDS             9923..11281
                     /locus_tag="PLF77_08035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPC08842.1"
                     /translation="MAKIPQKLERKKSDIYKDAPIAKFGERKPDFSTMGRKMKNPHAR
                     FREVVCVEACRTPYGRSGGALKNFSAMELGAMAIKEVLRRTGGKVAPSDVDYIFMGQV
                     VPAGCGQIPGRQATILAGVPEFVPSITVNKVCSSGIKTVDLAFQMILLGRAEICIAGG
                     QESMSNCPFVLPDMRWGAKMALPNGRVVDSMVYDGLWDAFYNRHMAIHGSEVADEFGF
                     SRQEQDEWALNSQMNAVRAMQEGRLDDEVFPVEVRQGKTILIMDKDEGPRPETTLEGL
                     AKLPPVFNHTSTVTGRPGSVTAGNAPGVNDGGDVCLLMSRAKADELGLKPIFTLVDYA
                     EVSQPTKDIATVPGLAIKKILDQNDMALDQMEVIEINEAFAAVALVSCRSILGMSRET
                     MFKKVNVNGGAIAYGHPIGATGARILMTLGYELRRRGGGWGVCGICSGHAQGDAMLIR
                     VD"
     gene            11503..>12170
                     /locus_tag="PLF77_08040"
     CDS             11503..>12170
                     /locus_tag="PLF77_08040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPC08843.1"
                     /translation="MAGNPDMSIVVPVYGSDITLMPLYCRTVAAVSPISENFEFILVD
                     DCGPGKPWEIIGAMAKKDSRVIGLKLAKNVGQHNAIKAGVDVAHGQWVVVMDCDLQDR
                     PEEIPRFWGKAQEGYNVVIGRRVERRDETIKRIFSCIFHGLLRWVTRQHSDAAQSNFG
                     IYSRQVIDKIKKLPEQSFYLPLLVRRAGFDVIPLDVEHDKRPYGKSGYTFSRRLSLAV
                     DILAS"
BASE COUNT         3007 a         2771 c         3370 g         3022 t
ORIGIN      
        1 ggggatcgaa accatgaatg atataacgat ggaacgccgt catgaagatc gccggcatga
       61 cgaccgtcga cgcggtgagc gccggaaata tgagcgccgc gaagacgtgc gcaaaatgaa
      121 tcaggaactt attcgcgagc aaatggaaaa ataccgcgtg ctgctcaagg atatcaagga
      181 tgcgtatttt gaggtcgatc ttctcggtaa tttcaccgtc gtcaacagcg cgatgtgcag
      241 tgctctggga tacggaccgg aggaattgat cggctcaaaa taccgtaaac tggtggacaa
      301 ggacggtgca aaaaaacttc agcaggccgt ggaacggctt cacaaaaacg acgaaccggt
      361 caatctgctg gacatggagg cggccaaaaa agacggaacg aaaattattt acgaggcgat
      421 ggtttccgtg atcagagaaa cggacggcgt ggtcgtggga ttcagagggg tcggccggga
      481 tgtgacccgc cgccggcaga tggaagctga attgaaaaga aatcagcacg atctgatcag
      541 aaagaatcag gaaatcgatc agagccggaa agacattcaa caggcgttgg tcagccttga
      601 aaaggctcat gaggaattga aggcgtcgca gttaaaaatc cttcagcagg agaagatggc
      661 atcgatcggg cagcttgccg ccggcgtagc gcatgaactg aacaatccca tgtcctttat
      721 ttccagcaat ctcagtacgc tggagaaata catcaaccgt ctgattcagt ttatccgcgt
      781 acagtccgag gccgtcgcat cgcttcagga tgccgccgtt gtgcgccgtg tcgatgagaa
      841 acgcgcagag gttaaactcg attacatggt cgaagatgtc cggggactgc tgaaagaatc
      901 tctggagggg gcgcaacggg tcaaaaaaat tgtctatgaa ctcaactcct tttcccgcat
      961 ggatgaagag gagtataaga aggcggatat tcatgagtgc atcgagagcg ccatcaccat
     1021 tgtctggaat gaattgaaat acaaatcaaa actcgaaaag cattacggcg acctgccggc
     1081 caccatgtgt tatccccggc agatgaatca ggtgttcgta aatctcctga tcaatgccgt
     1141 caacgcgatt gtcgataaag gaaccattac gattaagacg tggtacgaag accatgctat
     1201 ccgtattgat gtttcggata cgggtgccgg cataccgcgt gaaaatctga tccggatctt
     1261 cgaacctttt tttaccacca aagcagtggg gaaggggacc ggtctgggac tgagtattac
     1321 ctatgaaatt attcagcggc ataggggaga gataacggtt gccagtgagg aaggcagggg
     1381 cacaacgttt tcgataaaaa ttcctgttgt gtgaagaccg gaagattttt taaaaatatt
     1441 ccggtctgcc ggcggcgggg atgaatttcc gtttgacaca gaaagtgttc tgtggtttac
     1501 aagtcgttaa aaatattaag ctattggaaa gacagaccgg gcgaattttt aatcaaaggg
     1561 gttagtcgtt tcaccggcgt attgccggca ttggagcttt taagtttttt tattcgtttg
     1621 aatggttgtg gaataaactt aagtggaggt gtgtatgtcg aagaatattt tcagaattgt
     1681 ttttgtattg attttcagct ttttaacggc gggggtggtg ttggcggctc ctcccgtaaa
     1741 aatcggcgcg ctgtttgccg taaccggtcc ggcatcgccg ctgggcgagc cggaacgcaa
     1801 tacggcggtg atgatggttg atgaaatcaa caaagccggc ggaatcaagg gccgcaagct
     1861 ggaactgatt gtctatgata cgcagggaga tgccaccaaa gccgtccagg cggttaataa
     1921 actgatcaag gatgacaaag tcgtggcaat catcggtccg agcacgacag gcgacagcat
     1981 ggccgttatt ccggttattg aaaaatctca ggttccgctg atctcctgcg cggcgggcat
     2041 taaaatcacc gatccggtaa aaaaatgggt tttcaaaaca gcgcagaacg atgttctggc
     2101 ggtcatgcgg atttataaaa atttgcaaaa gcagaaaatt tccaaagtgg ctattctcac
     2161 ggtctccgac ggattcgggt cttccggacg cgaacagctg agattgcagg cgaaagaatt
     2221 cgatatcagc attatttccg atgaaacgta cggtccgaaa gacacggata tgaccgtgca
     2281 attgacaaaa atacgcaaca gcgcggcgca ggccgtcatc tgctggggga cgaaccccgg
     2341 gccggccatt gttgcccgaa acgccagaca gctggggatc aagattcccc tgtacatgag
     2401 tcacggtgtt tcttccaaga aattcattga actggccgga gaagccgcgg aaggcattat
     2461 gctgccttcc ggcaaggtga ttgtcgctga tcagttgccg gcttcagacc cgcagaaaaa
     2521 aaccctgttg aactatgtga gcaaatacaa ggagcgctat aaggtggaag gtgatcattt
     2581 cggcggccac gcatatgacg cagtcatgct gctgaagaga gccatggaac agggcggatt
     2641 cactcccgcg gccattcgcg accagttgga gaaaaccaaa aaatttgccg gtatcggcgg
     2701 aacgttcagt ttctcttccc gggatcatgc gggtctgacc agcgatgcct ttgttctggt
     2761 cagggtcgaa aagggcgact ggaaattaat caattaaccg atccggcatt cggattcatt
     2821 tttttataaa cggcgcggaa ccaaaaccgc gccgttttct gttcgtccag tgactcaaac
     2881 ataaattgat accatttcta aattaaaaat gatattgaat ccacgattgc ctgcgtttta
     2941 cagacggcgg aatcggcggc cacccggcct gtgtgacgcg tattcgatgc agagacggaa
     3001 tgggatatgg acaattttca acagctattt caatatgttc tgaccggttt atccaacggt
     3061 gccatttatg ccctgatcgg gttcggattt gccgtgattt ataattccac cggaatcatc
     3121 aatttcgccc aaggggaatt cgtgatgctc ggcggcatgc tgactgtttt cctgctgacc
     3181 gttttgcatg tgtcgctcgt gccggccatc gcactggcca ttgtcgtgtc gacccttgtc
     3241 ggaattttat tcgaacgttt cgccatccgg cctttaaaag acgctcaacc gctgagtctg
     3301 atcattatca cgatcggcgc cagtattttt atccgcggag tggcgatgct ggtttgggga
     3361 aaagatacac acgccctgcc cgctttttcc agtcacgatc ctctctcaat cgccggcgcc
     3421 acgattctgc ctcaacatct ctggatcctg ggcgtgacga ttttgatcat cgcggccagc
     3481 aggctatttt ttaatcacag catcatcggc aaagccatgc gcgcttgttc ctataatccg
     3541 cgcgcggccg ggcttgtcgg cattaatgtg aagagcatgg tgctgctttc tttttccctg
     3601 agcgccgcca tcggttctct ggccggtatt attatcgcgc ccctgacgat gacgtccttc
     3661 gatgtcgggg tcatgctcgg cctgaaggga ttttgcgcgg tcattatcgg cggcatgagc
     3721 agcggcatgg gaacggtgct gggcggtctt ttgctggggc tgctggaggc tctgggcgcc
     3781 ggttttattt ccgccagtta caaagatgcc atcgcgttta tcattctgct gttgattcta
     3841 tttattcgtc cggagggatt gttcagaaaa aaggaaacgg agcggtttta ggcgccggcc
     3901 gcggataaac cgtttacgga ttggtgcatt atatttgatc atgctaaaag aaaaaaaaca
     3961 tcagatattc atcttcgtca ttttcgcact ggccgtgttg tcggtcccgt ttttcctgca
     4021 ggggaattat cttttaaacg tgctcgtgtt tgtcggcatc aacaccatgc tggcggtggg
     4081 actgaatctg ctcttgggat atgccgggca gatttctctg gggcacgccg cctttttcgg
     4141 catgggcgct tacatctccg gcatcgtgac gtcccggtac cctgtcgagc cgtttctgat
     4201 catttttctg gcggcggtct gtgtcggcct gattgctttt gtgatcggat ttcccatttt
     4261 gaaactcaag gggcattatc tggccatggc caccctgggc ttcggcatta ttatgtacat
     4321 tattttcaat gaaaccgtgg actggacagg aggaccttcg ggcttgtccg gcattcccag
     4381 tttgaaaatc ggtccggttg ttttcgataa tgatcagagc aattattacc tggtgtgggt
     4441 ttttacgctg gtcgtgatga tgctctcgat taatttgtcc aactcgagga tcggccgcgc
     4501 acttcgcgcg atccatgatt cggaagtggc cgcacgcgtg atgggcgtca atgccaggtt
     4561 cctgaaggtg cagattttta cggtttctgc cgtgatttcg gccgttgccg gcagtcttta
     4621 cgctcacgtc atgacgttta tagcgcccgc gtcgttcggt tttaattttt ccgtggagct
     4681 gctgacgatg attgttatcg gtggtctggg cagcgtttac gggtcgtttc tgggggcggt
     4741 catcttgacc atgctgccgg aattcctgcg cgttttccag gatctggaca tcgttattta
     4801 cggattgatg ctgattctga tgacgatgta tatgcccggc ggtctgatca gcggaattga
     4861 gatggcggca gaattttttt cgtcccgcct gagaaaaggc aaaggtgaat gatgcttcga
     4921 ctcagcaaca tcacgcagat tttcggcgga ctgaacgcct tggaggatgt gtcgttttcg
     4981 gtcagggaag gatccattac cggcattatc ggtcccaacg gggccgggaa aaccacgctt
     5041 ttcaatatcg tgaccggcct ttacacccag accggagggg atgttttcct cggcggagaa
     5101 aatatttctt ttctggaaac cgaagaactt gccggccggg ggctggtgcg cacatttcaa
     5161 aatgtggaac tgttccggaa aatgaccgtt ctggaaaacg tgatggtcgg tttacacacg
     5221 aagagttcaa gcggcatttt ctcgtgcgct tttaaactgc cgcgtcagat cagggaagaa
     5281 aaagctatcc gggaaaaaag catggtatgg cttaaatata ccggcattgc cgagctggcg
     5341 gaaatgacgg ccggaaatct gcctttcggc aagggacggc tgctggaaat ctcccgggcg
     5401 ctggctgccg ggccgaagat cattctgatg gatgaaccgg cggcaggatt gaacagcagg
     5461 gagaccgccg aactggcgtt gcttattcgc aaaataagac aatccggcgt aacggtcgtt
     5521 ctggtagagc acgatatgga cctggtgatg gatgtctgtg atgctattgt ggtcttgaac
     5581 ctgggtaaaa aactggctga ggggacaccg cgggaaattc aggaaaaccc cgcagtcatc
     5641 tccgcgtatc tcggtgaagg ataatgaggt ttttaaaaaa ttgtccgatg ctgcagcgtg
     5701 caacaaaaat gcgtatcaac ccttgtctcg accgcgtcta atcgccggta tttttaggga
     5761 gccgttgggt aaacaccatg ttaaagataa agaatatcaa tacatattac ggacaggcgc
     5821 aggcgctgaa gaacgtttcg ctgcatcttg ccgagggcga aattgtcaca ctgatcggcg
     5881 ccaacggggc cggcaaaacc accctcctga attctttgtc aggggttgtg cccacgagaa
     5941 gcggtcagat tatcttcaac gacgcgccga tcaataatct tccggttcat cagattgtgc
     6001 ggcttggtat ttctcaggtt ccggaaggcc gtcaggtgtt taagcctgtc tccgtggaag
     6061 acaatctgga actggggttc tacctgcatc acaggatatt cggaggcgcc cgtgatgtta
     6121 aaaaaaataa agagatggtt tatgatctgt ttcccatcct ccgggagaga cgaaaacagc
     6181 tggccggaac cttgtccggc ggagagcagc agatgctggc tatcggccgc gctttcatga
     6241 cccggccgaa gctgatgctg ctggatgaac cttccatggg gctggcgccg atggtggtgc
     6301 aggagatttt ccgggtgatc gcctcgttgt cgagagaaca gaagaccacc gttcttctgg
     6361 tggaacaaaa tgcgcgcgcg gcgctgaaaa tggccggccg cggttatgtt ttggaaacag
     6421 gacacatgat cctggaaggg acagctgctg aactgctcaa caacaaagat gttcagcgag
     6481 cgtatctcgg cagggataaa aaagaaatat gggaaaggta agcagccgaa gtttggagaa
     6541 gaacggtccg tcaacgtcat caatattttt caacaacttt ccttttacgg aggttatttt
     6601 atgaatatat gggatcagac tcacgagtgt atggcgcgcg atgagctcga gcagctgcag
     6661 ctggaaagac ttcaggccgt ggtcaaccgg gtttacaaaa atgtcacgca ctatagaaaa
     6721 atatttaacg agacaggcat tatcgcggaa gatatccgtt ccctggccga tttgagccgg
     6781 ctgccgttta ccaccagaga cgatttacag gtcaattatc cctatgggat gtttgccgtt
     6841 ccgctcagag aggtggtgcg cattcattct tcctccggca ccaccagcaa gccggtcgtg
     6901 atggggtaca cgaaaaacga tatcaagata tggtcgaatc tggcggcgcg ttttatgacg
     6961 gctgccggtg tgactcccga tgatgtggtg cagattactt tccggtacgg acttttcacc
     7021 ggcgcgttcg gcgtgcatta cggggccgag gcgatcggtg cctcggtcat cccgatggga
     7081 accggaaata cggacaagca gattatgatc atgcaggatt ataaatcaac ggttctggtc
     7141 agcacatcca gttatgccat tgccctggcg gatcgtcttg atcaactggg tatcgatccc
     7201 aaatcccttt ccctgaagac gggactcttc ggtggcgagc cctggtcgga gaagatgcgc
     7261 gaagaaattg aaaacaggct tttgttgaaa gcgaccgaca actacgttat ttccgagctg
     7321 atcggtcccg gcgttgccgg tgaatgtttg tgcaaaaaag gcatgcatat ttatgaagat
     7381 gcctttattc cggagattat tgatcctgaa acgggggcgg tattgcctgc gggcgccgag
     7441 ggcgaactgg tgctcacgac gctgacccgg gaagcttttc cgatgatccg ttaccgcacg
     7501 ggggacatga cctcgttgga ctatgcgccg tgcgcgtgcg gccggacact ggtccgcatg
     7561 aaaaaaatca tgcgccgttc agacgatatg atcattgtcc gcggcgtcaa tgtttttccg
     7621 gcgcagatcg aagatgccct gttcagcgtg gctcaggggg aaacacccta tcagattgtt
     7681 gtggaaaggg aaggggccat ggacaatctg gaagtcgtgg tggaggtgac ggataaaata
     7741 ttttctctgg agttgcagaa acaaagatcg tttctggaaa cgattaaaaa acgtctggct
     7801 tcggtcacgg ggattaatgt cgttgtccgc ctggtggaat caaaaagcat tccgagacag
     7861 caaggccgga tccaacgggt gctggataaa cgacagatat aaacgacccc gggattatga
     7921 agggtgcttt tgaaccggtc gtcttttcgg gccggtctcc ggccggctgt gcttttcgat
     7981 attgcatgtg caggtaatcc gttcccgcgt cgcagtaagt cccagatcag caatcatctc
     8041 gtggtccagc gaactgtccc gtcccgggat tgtcagatca ttaccggtca tcagagcatt
     8101 ggcgcccgcc acaataccca ggggaagcaa ttgacgcaga tttttttcct ttccgccgca
     8161 gagccggatg tccctgtccg gaagcatgaa acggaaaacg gcgagggtca tcaggatttc
     8221 catgggcggt aacgctcgca tatgactcaa aggtgtgccc ttgaccggat gaaggatatt
     8281 cagaggaacg gagtcgacat caagttttct caaggtttga gccagttcaa tgcgatgaga
     8341 aatgttttcc cccatgccga tcagcgcgcc cgaacaaacc gacaaaccgg cttctttagc
     8401 cgcacagagg gttttgacgc tttcctcgta atcatgcgtg gtgcagacgg ttttgaaaaa
     8461 actccgcgat gtctccaggt tatgatgata acggaacagc ccggccgctt tgagctccat
     8521 agccgttgct ttgtcgatca ttcccagtga ggcacagggg aaaagacctg tttcgcggac
     8581 gccctgaatg gctctgatga tttccagcct ttcttttttc gactttaact gtttgccgct
     8641 ggttacgatg ccgaaacatt ccgctccgtt tctgaccgcc ttttcagcat cggcaatgat
     8701 ttgtgcggat tttttcagag gatacgagtg tgccgtcgtt ttgtaatgcg aggattgcgc
     8761 gcagaacatg cagtcttccg ggcatcgtcc ggatttggcg ttggtgattc cgcagaaaat
     8821 aattttcttt cccttgaaat gttcccggat ttcggaagcg gcggccagaa ccgaccaaca
     8881 gaagccggac gcttcaatga acagctccat agcctcggcc ggggtgatga aacgcgattg
     8941 ccggataatt ttgtttttta agtgatcgac gaaattcatt ttgactttct ctcttttttt
     9001 acatgaccga gtttggcccc ttcttttttc agtatgttaa tgagctggtc atgataataa
     9061 taaaccgcaa taaaaaccgt tacacccaga atcagaataa ccgtcagaag gatgtactgc
     9121 ccgttgcggg cgacgttgcc ggtaatcgaa agcatgatgg tgccgaaaat gcggccggct
     9181 gtggagatga taatgaacgt tccgaccggt atgcggctga ctcccatgat gtagcataga
     9241 taatccttgg gaaagccggg aatgagaaaa agcaggaagg agaccagcag acccttgtgt
     9301 tccataaagt gatcaaattt gtcaaaaacc tctttcttaa ccacattttc cagaagcggt
     9361 tccccaaaaa accgggccag catgaaggca atccatgacc caattgttag accgatggtt
     9421 gaataaagag ttccccacag cggtccgtaa agataaccgc cgatgatgcc ggtcagttcg
     9481 ccaggaatcg gggcggcaac gacctgaatg atctgcacaa gaataaaaat aaactggtcg
     9541 taaggatagg aaagaataaa attgaccagc ctgtcccggt ctcggaatag atggtgtaaa
     9601 tcataatgaa taaagatgat ggccgacaag gcaatgaaca aaaataaaaa gaaaattctg
     9661 taagccaggc gtttgtccat ggatggtttt gatccttaca attgcttgat ttgaaccggt
     9721 tggcgatttt ttatagcatt attctgcttt cgtcaatccc attattggat ccatccggcg
     9781 gtttttttgt tcatcttccg gaaaaatccg ttaaacccgt tgattttaaa aatgccctgt
     9841 gcaaaaacaa gtgaaaaatt tttcatcctg gtttataggg tgttttatga aaagcgaaac
     9901 acaaaaaggg gaggaaaatt gtatggctaa aatcccgcag aaactggaaa gaaaaaaatc
     9961 ggatatctat aaagacgcgc cgatagcgaa attcggagag agaaaaccgg acttttcaac
    10021 aatgggccgg aaaatgaaga atcctcatgc ccggtttcga gaggtggtct gtgtcgaggc
    10081 ctgtcgaacg ccttacggcc gttcgggcgg tgcgttaaag aatttttcgg ctatggagct
    10141 cggcgccatg gccattaaag aagtcttgcg gcgcaccggg ggcaaagtgg ccccgtcgga
    10201 tgttgactat atttttatgg gacaggtagt tcccgccggc tgcggccaga taccgggtcg
    10261 acaagctacg attcttgcgg gtgtgccgga atttgttccg tctataacgg tcaacaaggt
    10321 ctgttcgtcc ggcatcaaaa cagttgatct ggcttttcag atgattcttt tggggcgcgc
    10381 ggagatctgt attgcaggcg ggcaggaaag catgagcaac tgtccttttg ttctgccgga
    10441 catgcgctgg ggagcgaaaa tggctctgcc caacggacgg gttgtcgatt caatggttta
    10501 cgacggcctc tgggatgcgt tctataaccg gcatatggcc attcatggct cggaagttgc
    10561 ggatgaattc ggattttcca gacaggagca ggatgaatgg gcgcttaatt ctcagatgaa
    10621 cgcggtacgt gccatgcagg aaggcagact ggatgacgaa gtttttccgg ttgaagtccg
    10681 gcagggtaaa acaatattga tcatggacaa agacgagggg ccgcgtccgg aaacgacgct
    10741 cgagggtctg gccaaacttc ctcccgtatt taatcatacg agtaccgtta ccggccggcc
    10801 cggcagtgta accgccggga atgctcccgg cgtcaatgac ggcggagatg tttgtctgtt
    10861 aatgagcagg gcgaaagctg atgagctggg attgaagcct atttttaccc tcgtggatta
    10921 cgctgaagtt tcccaaccga ccaaggatat cgccaccgtt ccgggattgg ccatcaaaaa
    10981 gattctggat cagaatgaca tggcgcttga tcagatggag gtcattgaaa ttaatgaagc
    11041 ctttgccgca gtcgcactgg taagctgccg ttcgatactg ggcatgtcca gggaaacaat
    11101 gtttaaaaaa gtcaacgtca acggcggggc gattgcttac ggtcatccca tcggagcgac
    11161 cggtgcgcgg attttaatga cgttgggtta cgaattgaga agacgaggcg ggggctgggg
    11221 cgtctgcggg atttgttccg ggcacgcgca gggcgacgcc atgctgatca gggtggactg
    11281 aaatctgttc cgaaagactc ttgatggaaa tctcaggcgt tatgaaaaac caattcattt
    11341 ttgattgctt tttgcaaaag cggctttttc ttacagtccg gttttgaaaa aaaatcaaca
    11401 tacaaaagag ttgaaaatga tggtaagtta tgtaaaggtt catgtccaaa accttaaaat
    11461 atcagtaaat tttgttggtc aatcatttgc aaggggttct tgatggctgg aaatccggat
    11521 atgtccattg ttgtgccggt ctatggaagc gatatcaccc tgatgcctct ttattgtcgg
    11581 actgttgctg cggtcagccc gatttcggaa aatttcgaat ttattctggt cgatgactgc
    11641 ggaccgggaa aaccatggga gatcatcggc gccatggcga agaaggattc ccgggtaatc
    11701 ggtctgaaac ttgcgaagaa cgttggccag cacaatgcga ttaaagccgg ggtggatgtg
    11761 gcgcacggac aatgggtagt cgtcatggat tgtgatctgc aggaccgtcc ggaagagatt
    11821 ccccgcttct ggggaaaagc gcaggaagga tataatgttg tgatcggccg tcgggtggaa
    11881 aggcgcgatg aaaccatcaa gcgcatattt tcatgtatct tccacggtct tttgagatgg
    11941 gtaacccgcc agcacagcga tgcggcgcag tccaatttcg gcatttattc ccgacaggtt
    12001 attgataaaa taaaaaaatt gcctgaacaa tctttctatc tgccgttgct ggtcagacgg
    12061 gcgggcttcg acgtgatacc ccttgacgtg gaacacgata aacggcctta tggaaaatcg
    12121 ggctatacat tcagccgaag attatctctg gctgtggata ttcttgcttc
//
LOCUS       DAOOLB010000062        12057 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_5874_length_12057_cov_45.569072, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000062 DAOOLB010000000
VERSION     DAOOLB010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 12057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12057
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5874_length_12057_cov_45.569072"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="PLF77_08045"
     CDS             complement(<1..191)
                     /locus_tag="PLF77_08045"
                     /inference="COORDINATES: protein motif:HMM:NF014873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0F1 subunit beta"
                     /protein_id="HPC08844.1"
                     /translation="MESEQINQGKVLSVRSSVVDVYFSKVPPIRNVLKAGEKEDVIIE
                     VFTQLDNNTVRGVALTPTQG"
     gene            complement(222..1058)
                     /locus_tag="PLF77_08050"
     CDS             complement(222..1058)
                     /locus_tag="PLF77_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08845.1"
                     /translation="MEAYAAKLIDLIESKADDIARQWAEDVMKHNRTPSYHNLSKPMV
                     IEQGVDFYKLFRRMSMAEHPYEEAKTFSWKYAQKFYDEKIPLQEATYALILLRRNLWL
                     YAEFQGMFFTALEKQQAVESLNRTILMFDYVAYQVIQKYQERIHQDIDRRLFAVETLM
                     KKGCIGGEKKTYQYALMALFVVIAFVITFYYHSVVQTGVIFTHLFYIPIVLASICWGR
                     KGVWVSVFLAVMLLCSHAVFLEGVSFTEDIIRAVMFVVVGGVVGWLMEDVKKVRELFN
                     KR"
     gene            complement(1126..1596)
                     /locus_tag="PLF77_08055"
     CDS             complement(1126..1596)
                     /locus_tag="PLF77_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08846.1"
                     /translation="MKAFTAKLVDLTQTNADEIARQWARDVKKNAKTPSYHQAPEEKI
                     IRQAKEFYNNFQMMFFNESPYEQAKDFFDKYAEERYKEDIPLHEALYALILMRRHMWL
                     YAEFQSLFNVEVDHRQAVESLSRSMLMFDYIMYVVSMKFSKMMQQEVLSRSMKR"
     gene            complement(1946..3313)
                     /locus_tag="PLF77_08060"
     CDS             complement(1946..3313)
                     /locus_tag="PLF77_08060"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPC08847.1"
                     /translation="MRILIVDDEEVALNSIKRLLKWRGIRNVDTCANGKDAIAKIKET
                     DFDIVLLDLLMPEVDGLQVLEAAKPYRPHTEFIILTAIDDIPTTVKAIRLGAYDYLVK
                     PVDNDLLFLAIRRAYEHRGLLIGLSTTAGYDKSSIPAAFSEIVTQCPRMCSLLSYAQI
                     MAGGGNPIMITGESGTGKELMARGIHRAGPMPGGPFIAVNVSAIPASMFESQFFGHAK
                     GAFTGADTPHRGYFEQADGGTLFLDEIGELPVGLQSKLLRVLEEKSFYPLGASKPVWV
                     DVRVVSASNRDVEKACQDGSFRLDLFYRLKSVHIHLPPLREREGDIPLLALYFLRKSR
                     ENYKKTITGFNPEAMELLTARNYPGNIRELAQVVENAVLLADSDFIQPCHLGMVSTAP
                     LPLSRQLGTLKENEESHLVYVLKNVQGDRGRAAQILGITVRQLQRKIAAMKKNPQWET
                     ILNDI"
     gene            complement(3310..5451)
                     /locus_tag="PLF77_08065"
     CDS             complement(3310..5451)
                     /locus_tag="PLF77_08065"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC08848.1"
                     /translation="MKTGSSPSRKNKSPRRHESVWTGEVFSNGPQLLQSIIGSSPIPA
                     FIIGKDHLIIYWNRALEELSGIKSKEVMGTGQQWRAFYKTARPCMADLLVDKYQKKIP
                     QWYSGKFIKSKLIDGAYEATDFFPELGKRGKWLRFTSAVIRNSKGSVIGAIETLEDIT
                     QAKRAEEALLKAHEDLEIKVRERTRKLTKANKALKEAMDHLSLILEFLPIVSYTCNAD
                     ESMAITFVSNRVKEITGYTPAQFTANHPFWRNHIHPEDQSNVLKKLKPTARKKTYQLE
                     YRLQIADGSYRWISDHRRLIKPGKHSRSYMVGAWQDITEDKRIRHEGEFRLQQMIQSH
                     KLKALGEVVAGVAHEINNPVSFIANNITMLEEMWNAVEPILASDGASHPNWSEKGIGY
                     VEVCTNMKEIIEEFKTASQRIKRVVSGLKEFARTDETVEKKPVKIEEVIQGVMIIVGA
                     QVRKTVSRIDLYIDSNLPPVQGHFQKIEQVIANLLINAHQAITPERKGRIVITARSLD
                     RLNAILIEIEDNGIGMKKAVLDHIFQPFFTTRRDMEGTGLGLSISYGLIKEHNGLIGV
                     LSKPGVGSRFSIYLPLDARTPISIRPAVLCCDSDTAYLKEIKMSIVNVIDWQCGEQDD
                     CDDIIAYLEMHPEVDIFISEINLPVMNGWDLSRKIKERFPLLCVILYSSDPQAGKPEG
                     RMSGKPDYVLQKPFSMTQLQTIIRETGRQRL"
     gene            complement(5666..6145)
                     /locus_tag="PLF77_08070"
                     /pseudo
     CDS             complement(5666..6145)
                     /locus_tag="PLF77_08070"
                     /inference="COORDINATES: protein motif:HMM:NF022168.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YrbL family protein"
     gene            complement(6406..7146)
                     /locus_tag="PLF77_08075"
     CDS             complement(6406..7146)
                     /locus_tag="PLF77_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08849.1"
                     /translation="MKKLFLSILVIILTISFMSWVIYLNQVPAIKQASDSKDIVIEPS
                     PRSDDETAQRALLPDEKSKEISADQGAPATGKSVPAKDRAQVVPKSTAKKPAVSAVDE
                     TKKAPPVSGETLKKPQPAPSEKIQATPASEKSQKEMSRLQDQCEKRSKKIFRKAYGNG
                     VVEKNQNVYLYLYKSHYNKKRDQCFMLVTEDGVLERYKKLLDVDENESLGSVRLNNER
                     KNLGCYLLNSKCDSEETWDLLAKPYMEE"
     gene            complement(7509..7742)
                     /locus_tag="PLF77_08080"
     CDS             complement(7509..7742)
                     /locus_tag="PLF77_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HPC08850.1"
                     /translation="MKDKKTYAVVLFKAVSYALKAEKILKQEGLPHKLIPVPKQISSD
                     CGVCLRFEQNIQNQIEAALLNKVEIEDIITLDH"
     gene            complement(7739..8350)
                     /gene="yedF"
                     /locus_tag="PLF77_08085"
     CDS             complement(7739..8350)
                     /gene="yedF"
                     /locus_tag="PLF77_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HPC08851.1"
                     /translation="MADIKIIDARGLSCPQPVILTKKALEDNARVTTVVDNETALENV
                     KRLAGKFGCAVTVEPKGKSVYEIHIVRGTDFNKDQDFAASCQTGTAPSGPFVIAVTED
                     KMGRGSDELGTVLIKSFVHTVADLGKKPDVMIFYNTGVKLTIEGSDVLEDLKQMNSEG
                     VQILVCGTCLNYFEIKDKLAVGIVSNMYDIVETMSSAGRLLAP"
     gene            complement(8352..9509)
                     /locus_tag="PLF77_08090"
     CDS             complement(8352..9509)
                     /locus_tag="PLF77_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HPC08852.1"
                     /translation="MSIIYFDNAATSWPKPPEVNKAIQKYLKKIGANPGRSGHRLSVE
                     AARVIYDTREKLAQLFNITDPLQIVMTKNATEGLNIAVSGLLKSGDHVITSSMEHNSV
                     MRPLRVMERRGVELTVIPCDREGLIDPAHVNGAVKKNTRAIFMTHASNVTGTVMPVAD
                     IGRIARERNLVFCVDAAQTAGSYPIDVVKMNIDLLAFTGHKSLFGPSGTGGIYIREGL
                     ENKIEPLCVGGTGSRSEVEEQPDFMPDRYEAGTPNTVGIAGLQAGVDFVLSKGVSEIK
                     HKEDNLVEIFMDGVRDVPGMVVYGKKDAQKRTPVVSFNIAGMDPAAVALELDDRFNIM
                     ARSGIHCAPSAHKTIGTYPQGTVRFSFSYFNTQEQIAKAIDAISKISRRKK"
     assembly_gap    9625..9724
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9725..10875)
                     /locus_tag="PLF77_08095"
     CDS             complement(<9725..10875)
                     /locus_tag="PLF77_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HPC08853.1"
                     /translation="MREGVEPLVNDGFAGDAAKRCLSYIQSQREKGKNVVGIYCGYAP
                     MEVIRAADIVPAVLCAFADKTIAAGETVLPANLCPLIKSSYGFIRTDTCPFYALSEAV
                     IAETTCDGKKKMFELISDIKPTHVMDLPQLPDQKEAEDNWTTMIRKLAGFLETTFKTK
                     ISDDRVEQEIKNTNLKNKLLNQIFDFAALPSVPLTWQEVYDLTYFGQVATTEDMLSML
                     EASLAKLQKRVADGVYHGKKDSPRVLVSGCPVGGDATKVFNIIEEAGGVIVYLDACTG
                     MKAYAGHFEEGAPDPYRSVSKRYLDVPCSCMTPNDRRLSELDLIIGKFKPDVIIDVVL
                     HACHSYNIESSKVKTHVETRHKKPFLKIETDYSQGDVEQIRTRVEAVLET"
     gene            11132..>12057
                     /locus_tag="PLF77_08100"
     CDS             11132..>12057
                     /locus_tag="PLF77_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPC08854.1"
                     /translation="MGDYTKMWTELGLDLKGHDALLSVLGGAYTNIFLSQKNRPEGMK
                     YFDFVMSEVHGLRIQELMDAKAQGRKVIGSYCVFVPEELILAVDGVSVGLCAGAEFNF
                     EGAETMLPRNTCALIKSAFGFKLGQVCPYLEASDVVIGENTCDGKKKSYEMLAPLVKD
                     LYVMDMPQMKSESGRTLLKEEYRKLIDKLEKVSGKKVTVESLQKGINIVNAKRDAVRR
                     LTELRAADPAPISGLDALLVNQVFFYDDPVRFTDSVNKLCDELEDRVKKGIGIFPKGT
                     TRVIVSGCPMAVPNWKLPMLIESVNAVIVGEES"
BASE COUNT         2941 a         3001 c         2712 g         3303 t
ORIGIN      
        1 ccctgcgtgg gagtcagggc gacgccccgg accgtattgt tgtcgagctg ggtaaagacc
       61 tcgatgatga cgtcctcttt ttcgccagct ttgagaacat tacgaatcgg gggcaccttg
      121 gaaaaataaa catcaacaac actgctgcgg acggataaaa cttttccctg attgatctgt
      181 tcgctttcca cgttcatctc ctcttaaata aaattgatgc tttaacgttt attgaaaagt
      241 tctcttactt ttttcacatc ttccatcaac catcccacca cgccgccgac cacaacaaac
      301 attacggccc gaatgatgtc ctccgtaaac gaaacgcctt ccagaaaaac agcgtgactg
      361 caaagaagca tgacggcgag aaacacagac acccagacac cctttctgcc ccagcaaatg
      421 gaggccagaa caatgggaat gtagaacaga tgggtaaaaa taaccccggt ttgaaccaca
      481 gagtggtaat agaatgtgat gacgaaagcg atgacgacaa aaagggccat cagcgcgtac
      541 tgataggttt tcttttcgcc cccgatgcag ccctttttca tcagcgtttc cacggcgaaa
      601 agtcttctgt ctatgtcctg atgaatgcgt tcctgatatt tctgtatgac ctgataggcc
      661 acataatcaa acatcagaat ggttctattc aggctctcga cggcctgttg tttctccaga
      721 gcagtgaaaa acattccctg aaattcagca tacagccaaa ggttcctgcg caagaggatc
      781 agggcgtaag tcgcttcctg aaggggaatc ttttcatcgt aaaatttttg ggcgtatttc
      841 cacgaaaatg ttttggcttc ttcatagggg tgttccgcca tggacatgcg gcggaacagt
      901 ttgtagaaat cgactccctg ttcaattacc atcggtttcg acaggttatg gtaggatggg
      961 gtccggttgt gtttcatcac atcctcggcc cattgccgcg caatatcgtc ggctttgctt
     1021 tcaatcagat caataagttt ggcggcgtaa gcttccatgg cttcatctct tctaatacaa
     1081 accggcttga taaatcatcg actgcgtccg gctgacgtct gcttttcagc gtttcattga
     1141 tcttgacaaa acctcctgct gcatcatttt tgaaaatttc atggaaacaa cgtacataat
     1201 gtaatcgaac atcagcatcg atctgcttaa gctttccacg gcctgccgat gatccacctc
     1261 cacattgaac agggactgaa attccgcata gagccacatg tgtcttctca tcagaatcaa
     1321 cgcgtacagg gcctcgtgaa gcgggatgtc ttctttgtat ctttcctccg catacttatc
     1381 gaaaaaatct tttgcctgtt cataaggaga ctcgttaaaa aacatcattt gaaaattatt
     1441 atagaattcc ttagcctgac gaataatctt ctcttccggt gcctgatggt agctgggagt
     1501 tttcgcattc ttttttacgt ctctggccca ctgcctggct atttcgtccg cgtttgtttg
     1561 agttaaatcc acaagctttg cggtaaaagc tttcattgct catcatcctt ttcaaaagaa
     1621 taaacagcaa cgggcatgcc aaaactattt tcccctcttg atttattcgt gaccggcttg
     1681 tctatccgga caaaaagcga gacgaaaatc atcagaaacc tgtgctattg aagccttacg
     1741 aaaaagcgga tggaaaaatc tctccggtaa aaaaatattt tttctttgtc atgtcagcgg
     1801 atcaggtttt tgaacggacg agaaaaaaac gaaataaaag acggcggaaa ggatatcgat
     1861 ggacaggccg gggactcttt aaaacggcaa gaccttttaa aaacaggaac ttattcaaaa
     1921 agacatttat gtcgcccctg acgacttaaa tgtcattcag gatggtttcc cattgcggat
     1981 ttttcttcat cgcggcaatt tttctttgca gttggcggac ggttatgccc aggatttgcg
     2041 ccgccctacc gcggtcgccc tgaacatttt tcaaaacgta caccagatgg gattcttcgt
     2101 tctccttgag cgtacccagt tgccgggata aagggagcgg tgcggtggaa accatcccca
     2161 gatgacacgg ctggatgaaa tcggaatccg ccagtaaaac ggcgttctca acaacctgcg
     2221 ccagttccct gatattgccc ggataatttc tggcggttaa cagttccatg gcttccggat
     2281 taaagcccgt aatggttttt ttataattct cacgcgattt gcgcagaaaa tagagcgcga
     2341 gcagagggat gtctccctct ctttctctca acggcggcaa atggatgtgc acggatttca
     2401 aacggtaaaa aagatccagc cggaaacttc cgtcctggca cgccttctcg acatctcggt
     2461 tggaagcgga gacaacccgg acatccaccc agaccggctt ggaagcgccc aggggataga
     2521 aagacttttc ttccagtacc cgcagcaatt tggactgcag gccaacgggc agttccccga
     2581 tttcatccag aaaaagggtc cctccgtccg cctgttcgaa atagccgcga tggggagtat
     2641 ccgcgccggt gaatgcgcct ttggcatggc cgaagaactg gctttcgaac atgctggcgg
     2701 gaatcgcgct gacgttcacg gcaataaaag gaccgccggg catcggcccc gcccgatgaa
     2761 tccctctggc catcagctcc ttccctgtcc cggactcgcc ggtaatcatg atcgggttgc
     2821 cgcctccggc catgatctgt gcataggaaa gaagcgaaca catcctcgga cactgggtca
     2881 caatctccga aaatgcggcg ggaatactgc ttttatcgta acctgccgtg gtggataagc
     2941 cgatgagcag cccccggtgt tcgtaggccc gtcggatggc cagaaaaagc aagtcattat
     3001 ccaccggttt aaccagataa tcatacgcac cgagacgaat tgctttcacc gtggtgggaa
     3061 tatcatcgat agcggtgaga ataatgaatt ccgtatgcgg ccgatagggt tttgccgctt
     3121 ccaggacctg caaaccgtca acttccggca tcagcagatc cagaagaaca atatcgaaat
     3181 cggtttcctt gattttcgca atcgcgtctt tgccgtttgc acaggtgtcc acatttctga
     3241 tgccccgcca cttcaaaaga cgtttgattg aattcagagc aacctcttca tcatctacaa
     3301 tcagtattct cataatcgtt gcctgcctgt ttcacgtatg atcgtttgca attgcgtcat
     3361 gctgaagggt ttttggagca cataatccgg ttttcccgac atgcgtccct cgggtttgcc
     3421 tgcctgcgga tcggaggaat aaagaatgac acagagcagg gggaaacgct ctttgatttt
     3481 tcgggacaaa tcccatccat tcatgaccgg cagattaatt tccgaaataa aaatgtcaac
     3541 ttcgggatgc atttccagat aagcgatgat atcatcgcaa tcatcctgct cgccgcactg
     3601 ccagtcaatg acattcacaa tgctcatttt tatttctttc aaataggcgg tatcgctgtc
     3661 gcagcagaga acggccgggc gaatgctgat cggtgtgcgg gcatccagcg gaaggtagat
     3721 tgaaaaacgg cttcccactc ccggcttgga cagcactccg atcaatccgt tatgctcctt
     3781 gatcagaccg taagagatgg aaagtcccag tccggtcccc tccatatccc ggcgcgttgt
     3841 aaaaaagggc tggaaaatat gatccagtac cgcctttttc atgccgatgc cgttatcttc
     3901 aatttcaatc agaatcgcgt tgagccgatc aagacttctg gcggtaataa caattcttcc
     3961 ttttcgttcc ggtgtaatgg cctgatgcgc attgatcagc aggttggcga tcacctgttc
     4021 gattttctgg aaatggccct ggacaggcgg taaattgctg tcgatgtaaa gatcgatgcg
     4081 ggaaacggtt ttgcgaacct gcgcaccgac aataatcatc accccctgaa tgacttcttc
     4141 gattttcacc ggttttttct ccacggtttc gtcggtgcgg gcaaattcct tcagaccgga
     4201 aacaacgcgc ttgattcttt gggacgccgt tttgaattcc tcgatgattt ccttcatgtt
     4261 cgtacagact tccacatagc caatgccctt ttcactccag ttgggatgag acgcgccgtc
     4321 actggccaga atcggttcca ccgcgttcca catttcctcc agcatggtga tgttgttcgc
     4381 gatgaaactg accgggttgt ttatttcatg agcaacgccc gcgaccactt cgccgagcgc
     4441 tttcaattta tgggattgaa tcatctgctg taaacgaaat tctccttcat ggcggattct
     4501 tttatcttct gttatatcct gccatgctcc caccatgtaa ctgcgcgagt gtttgcccgg
     4561 tttgatcaaa cggcgatgat ccgatatcca gcggtaagaa ccgtcagcga tctgtaagcg
     4621 gtactccaac tggtaagttt ttttcctggc agtcggtttc agttttttca gaacattgct
     4681 ctggtcttcg ggatgaatgt gattccgcca gaaaggatga ttggccgtaa attgagcggg
     4741 cgtgtatccg gtgatttcct tgacgcgatt actgacaaac gtgatggcca tgctttcgtc
     4801 ggcattgcat gtgtaggaga cgatgggaag aaattccagg atcagggata aatgatccat
     4861 ggcttctttc agagccttgt tggccttggt cagttttctc gtgcgttccc tgactttgat
     4921 ttccaggtct tcatgcgctt tcagcagcgc ttcttccgct cgctttgcct gcgtaatgtc
     4981 ttcaagggtt tctatcgctc cgataaccga tcccttggaa tttcggatga cggcggaagt
     5041 aaaacgcaac cattttcctc tcttgcccag ttccgggaaa aagtccgtcg cttcatatgc
     5101 accatcaatc aattttgatt tgataaactt accggaatac cattggggaa ttttcttctg
     5161 atatttatca accagcagat ccgccatgca cggcctggcc gttttataaa acgctcgcca
     5221 ctgttgtccg gtgcccatca cttccttcga cttgattccg cttaattctt cgagggccct
     5281 gttccagtag ataatcagat gatctttccc gatgataaag gcggggatcg gagaactccc
     5341 gataatgctc tgcagcaatt gcggaccatt gctgaatact tcgccggtcc agacgctttc
     5401 atgacgtctg gggcttttat tttttctgga cgggctgctt ccggttttca tcttctgcta
     5461 ccgaaaatac gactgacgcg tgcgtttgaa ctattaaatg atgtattaac tctgctaaag
     5521 caattatccg cggtgaaggt gacgatttga aaaatgttcg gtatggtttg attatataga
     5581 acgggaaact gatgtcaaga aaattaaaaa cgaaaaacgc attgcttttg ccgacaatct
     5641 gaatcgtact gcatgcaaca acgtttcagc gcgacgaacg ccgcgccgac cgctccttca
     5701 gcatccgcgc cagggctttg tttcctttga aggcgttcag gaggctgtct tcaaaacgct
     5761 gccattttct gtctatttta cgttttgcaa aaaaccggca gtaattgctg acgggaatga
     5821 atgcggaatt atatatatta tcgatcagaa ccaatcgggc aatcccggat gcatccatcc
     5881 ggcaaagaat attgtagggt ttgatgtcca tggttacgat gcggttctgc aggagatatt
     5941 ttttcagccg gcataatgca tcggaaaggc tggaaaagta tttttcggtt tgttcgtctg
     6001 aggataaata gtattccagg cttttggcaa cggtcccgtc atgatccata atcagatcga
     6061 aaacagcccc ctgcccgaga tcggtttcga catctccatg ataccgggaa atcatctccc
     6121 gggaaattcc atgttttcga agatgccgat aatatttttt tcccgtttaa tttccagagg
     6181 gctgtctttt accgtgattt ttatgcagag gttttgatcc tccggatgtt tatagcattc
     6241 ccgatgcctg ccccttccaa tcaaggttgt cctgtctatg taaagcatta ctctaccgtt
     6301 atgatccagg tgatccgtcg cctgccgctt aaaaatccgc agcaacttaa tcgaggggca
     6361 ggaaaaacag ggaaaaaact caaatttata atttcagaaa ttctattact cttccatata
     6421 aggttttgcg agcaaatccc acgtttcttc cgaatcacat ttgctgttta aaagataaca
     6481 gccgagattt tttctttcat tgtttaaccg gacgcttccc agactttcgt tctcatcaac
     6541 atcgagtaat tttttatatc gttccaatac gccgtcttcc gtgacgagca taaaacattg
     6601 atcccgtttt ttattgtaat ggctcttata taaatacaga tagacatttt gatttttctc
     6661 cacgacgcca ttgccatacg ctttacgaaa tatttttttg ctccgttttt cgcattgatc
     6721 ctgcaatctg gacatctctt tttgactttt ttctgaagct ggcgttgctt gtatcttttc
     6781 cgacggggcc ggttgaggtt ttttgagcgt ctctccggaa accggcgggg ctttttttgt
     6841 ctcatcaacg gccgaaaccg caggtttttt tgccgttgat tttggtacga cctgtgccct
     6901 gtctttggca ggcaccgatt ttcccgttgc cggggcaccc tgatcagccg aaatttcttt
     6961 tgacttttca tcaggcagca gcgcccgttg tgctgtttca tcgtcagaac gcggcgatgg
     7021 ttctatgacg atatcctttg agtccgacgc ttgttttatc gcggggactt gattcaaata
     7081 gatgacccac gacataaacg atatcgtcag aataataaca agaatggaca gaaaaagttt
     7141 tttcacggcg ttttcctctt tcagatcata aggttaaaaa tttaaaacca acaattggtc
     7201 aggattatat ttgtgatatt ttaattttta cactgaacga acaatttagg agttgtgtac
     7261 atatcagatt ttcactgatt ttcaaaataa attttatcat ttttcaaaaa cggccgcgct
     7321 tcccgtacag aataagattc acgatgcctt cggcggcaat catttttacc cggaaagtaa
     7381 attcattgag ggagaagcgg actttataga aggatgcttc cggacgagat gaagttattg
     7441 ttccataagc agacaatctt tcccgcatcc ggagcacgaa catatatttt ttcgttatcg
     7501 gctgatggct aatgatccag tgtgatgatg tcctctattt ccactttgtt gagaagtgcg
     7561 gcttcgattt gattctgaat gttttgttca aaccgcaggc agactccgca gtccgagctg
     7621 atctgcttgg gcacgggaat caatttgtgc ggcaagcctt cttgcttgag aattttttcc
     7681 gccttcaagg catagctcac cgctttgaac agaacgacgg catatgtttt tttgtctttc
     7741 atggcgcaag caatcgtccc gcactgctca tggtctctac aatgtcatac atattggaga
     7801 caatccccac ggccagctta tccttgattt caaaataatt gaggcaggtg ccgcaaacca
     7861 gaatctgaac gccttcgctg ttcatctgtt ttaagtcctc cagaacatcg gaaccttcaa
     7921 tcgtcaattt cacaccggta ttgtagaaaa tcatcacatc cggttttttg cccaggtccg
     7981 caacggtatg cacaaaggat ttgatgagaa ccgttcccag ttcatcgctg ccccgtccca
     8041 ttttatcttc cgtcacggct atgacaaaag gaccggatgg agccgtgcct gtttgacagg
     8101 aagcagcgaa atcctgatct ttattgaaat ccgtcccgcg aacgatgtga atctcataaa
     8161 cacttttgcc tttgggttcg acagtcacag cgcagccgaa cttgccggcc agacgcttca
     8221 cgttttccag agcagtttcg ttatcaacga cggtcgtcac cctggcgttg tcctccaatg
     8281 ccttttttgt cagaataacc ggttgaggac aggaaagccc ccgggcatca atgattttaa
     8341 tgtctgccat attatttttt cctcctgctt attttgctta ttgcgtcaat ggcttttgct
     8401 atttgctcct gtgtgttgaa atagctgaag ctgaagcgca ccgttccttg aggataggtg
     8461 ccgatggttt tatgagcgga tggcgcgcaa tggatgccgg aacgcgccat gatattaaag
     8521 cggtcatcga gttccagagc gaccgcggcc ggatccatcc cggcgatatt gaaggaaacg
     8581 acaggggttc gtttttgtgc atcttttttc ccgtaaacga ccatgccagg cacatcccga
     8641 acgccgtcca taaatatttc caccaggttg tcttccttat gcttaatttc gcttacacct
     8701 ttagataaaa caaaatcaac tcccgcctgc aaaccggcta tgcctaccgt attgggcgtc
     8761 cccgcttcat aacggtcggg catgaagtca ggctgttctt ccacttccga gcggctcccc
     8821 gtcccgccga cacaaagggg ttcgatctta ttttccagac cttcacgaat atagatcccg
     8881 ccagttcccg aaggaccaaa taaagattta tgtcctgtaa acgccagcaa atcgatattc
     8941 atttttacaa catcaatagg atagctgccc gccgtctgcg cagcatcaac acaaaaaacc
     9001 aagttgcgtt cccgcgcgat acggccgata tccgcaacgg gcatcactgt tcccgtgaca
     9061 ttggaagcgt gggtcataaa aatcgctctg gtattttttt tgaccgctcc gtttacgtgc
     9121 gccgggtcaa tcaagccttc ccgatcgcag ggaatgacag tcagttcgac acctctgcgc
     9181 tccattaccc gcaggggtct catcaccgaa ttatgttcca tactggatgt aataacatga
     9241 tcgcctgatt tcagcaggcc ggagaccgca atattcaatc cttccgtggc atttttcgtc
     9301 atcacgatct gcagaggatc ggttatgttg aaaagttgcg cgagtttttc ccgtgtgtcg
     9361 taaatgacgc gcgctgcttc aaccgacagg cgatgaccgg aacggccggg atttgcgccg
     9421 atttttttca ggtatttttg tatggccttg ttgacttccg gcggcttcgg ccaggatgtc
     9481 gcagcattat caaaataaat gatactcatc gattaacctt acaattgaac gtaaatcttt
     9541 ttctttatgc ttcaggttgt cttttaacat tcaaactgaa actaaaaaag cactttcttt
     9601 tttttccatt caccattcac tattnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9721 nnnngtttcg agcaccgcct ccacgcgagt acggatttgc tccacatcgc cctgcgaata
     9781 atccgtttca attttcaaaa acggcttttt atgtctggtt tccacgtgcg tcttcacctt
     9841 ggatgattcg atattatacg aatgacatgc gtgcaacaca acgtcaataa taacatccgg
     9901 tttgaacttg ccgatgataa gatcgagttc cgaaagcctc cggtcattag gcgtcataca
     9961 ggaacagggc acgtcgagat accgtttgga aaccgaccgg tagggatccg gcgcgccttc
    10021 ctcgaaatgt cccgcatagg ccttcatgcc agtacaggca tccagataaa cgatcactcc
    10081 gcctgcttct tcaatgatgt taaaaacctt ggtggcatcg ccgccaaccg ggcaccccga
    10141 aacgagcaca cgaggtgaat cttttttccc gtgataaacg ccgtcggcaa cccgtttttg
    10201 aagtttggcc agtgaggctt ctaacatcga cagcatatct tcagtggtgg ctacctgccc
    10261 gaaatacgtc agatcgtaaa cttcctgcca ggtcaaaggc acagacggaa gagccgcaaa
    10321 atcaaaaatc tgattgagca acttgttttt gagatttgta tttttaattt cctgctcgac
    10381 ccggtcgtcc gatattttcg tcttgaatgt cgtctccaga aaaccagcga gctttctgat
    10441 catcgtcgtc cagttgtcct cggcttcctt ctgatccggg agctgcggca gatccatcac
    10501 atgcgtcggt ttgatgtccg aaatcagctc gaacattttt ttcttgccgt cgcaggtcgt
    10561 ctcggcaatg accgcctcag aaagggcata aaacggacag gtgtccgtcc tgataaagcc
    10621 gtagctcgat ttgatgagcg gacagagatt ggccggcagc accgtctcgc ccgcggcaat
    10681 ggttttatcg gcaaaagcgc acaaaacggc aggcacaatg tccgcggcgc gtattacttc
    10741 catcggcgcg tacccgcaat agatccccac tacatttttc cctttttcac gctgggattg
    10801 gatataactc aagcaccgtt tcgccgcatc cccggcaaaa ccgtcattaa ccagcggctc
    10861 aactccttct ctcatactgc tgcctcccgt aatgtcttgc cgattagcta tatgatccac
    10921 cgtcatatat ccaatagata atagttataa tccccgacgc attatttctt ttattcattg
    10981 gcgcaatcca aatcacaatg atacgaaggc gaagcttgtg caacccgcgc aacgaacatc
    11041 cggacttctt cacttttctg acgtgtgcgg ttaacggtca aacatttcat cgatccggat
    11101 aaaagactaa aataacggtt ggaggtttat tatgggggat tatacaaaga tgtggacgga
    11161 gctgggactt gacctgaaag gtcatgatgc gcttctttcc gttcttggcg gcgcttacac
    11221 aaacattttt ctttcacaaa aaaatcgtcc cgagggcatg aaatattttg attttgtcat
    11281 gagtgaagtg cacgggctgc gcattcagga actgatggat gccaaggccc aaggcagaaa
    11341 agtgatcggt tcctattgcg tttttgttcc ggaggaattg atcctggccg ttgacggcgt
    11401 atccgtgggt ctgtgcgccg gagcggaatt caattttgaa ggcgcggaaa cgatgcttcc
    11461 ccgaaacacc tgcgcgctca tcaaatccgc cttcgggttt aaactgggcc aggtctgtcc
    11521 gtacctggaa gccagcgatg ttgtcatcgg tgaaaacacc tgcgacggca agaagaaatc
    11581 ctatgaaatg ctcgcgccgc tggtcaagga tctttacgtg atggacatgc cccagatgaa
    11641 atcggagtcg ggcaggacgc tgctcaaaga ggaataccga aaactcatcg acaaactgga
    11701 aaaagtcagc gggaaaaaag ttaccgtgga atccctgcaa aaaggcatca acatcgtcaa
    11761 cgccaaacgt gacgccgtcc ggagactgac cgaactgcgc gccgctgatc cggcgccgat
    11821 atccggactg gatgcccttc tggtcaatca ggtctttttc tatgacgatc cggtgcgttt
    11881 taccgattca gtcaacaaac tctgtgatga actggaagat cgcgtgaaaa aaggcatcgg
    11941 aatcttcccg aagggcacaa cgcgagtgat cgtatccggc tgccccatgg ccgtccccaa
    12001 ctggaaactg cccatgctca tcgaatccgt caacgcggtc atcgtgggcg aagaatc
//
LOCUS       DAOOLB010000063        11885 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_5979_length_11885_cov_42.969062, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000063 DAOOLB010000000
VERSION     DAOOLB010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5979_length_11885_cov_42.969062"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="PLF77_08105"
     CDS             complement(<1..202)
                     /locus_tag="PLF77_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HPC08855.1"
                     /translation="MRLFPKEENFFELFDELADKIEEGGKLFLEMAQARDYSDVRVAK
                     LKEIEHEADGITHKTYEKMHKTF"
     gene            complement(338..823)
                     /locus_tag="PLF77_08110"
     CDS             complement(338..823)
                     /locus_tag="PLF77_08110"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC08856.1"
                     /translation="MKKFIYRQQIKPSDIEVIADMVESSGFFSDEEIEIAIELAEDKL
                     DKQEVSSYQFLFAEIENQVVGYTCYGLIPATAGSYDLYWIVVAKDLQGLGVGKMLMAE
                     TENRIYQNGGRQIYAETSSRAQYTPTHKFYESCGYAKEAFLKNFYSDGDGKIIYAKVL
                     K"
     gene            complement(832..1179)
                     /locus_tag="PLF77_08115"
     CDS             complement(832..1179)
                     /locus_tag="PLF77_08115"
                     /inference="COORDINATES: protein motif:HMM:NF016245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPC08857.1"
                     /translation="MLKTIIFLFFSNLFMTFAWYGHLKNFNHKPLLFVILFSWGVAFF
                     EYVLQVPANRIGFHYYSLGQLKVIQEVITMIVFAGFAIMYMKQPLKMDFLWAGLCLVG
                     AVYFMFREGLPHT"
     gene            complement(1185..2186)
                     /locus_tag="PLF77_08120"
     CDS             complement(1185..2186)
                     /locus_tag="PLF77_08120"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08858.1"
                     /translation="MKKVVILHSDIATDGSLDELDCLVQAEAIADAVRTLHYEPVLLP
                     FELNLNRTITMLQSLKPAVVFNIVETLDSKGSLIHFAPAVLDVLQIPYTGCPTHAIYL
                     TSNKPTAKKIMRDAGIATPDWLEQNGIIFRQDDGGKFLIKSSWEHASIGLDENSLISY
                     TDKTAIIKEMKLREKKLGGSCYAEAYIDGREFNVALMSDQAGARVLPIAEMLFQDYAP
                     DKLKIVDYKAKWDADSFEYNNTVRKFNFDDEDALLISSLQDIALQCWNIFSLRGYARV
                     DFRVDKNNKPWVLEVNTNPCLSPDAGFAAALERAEIKYHEAIGLIIDNALKSISKEI"
     gene            complement(2192..3211)
                     /locus_tag="PLF77_08125"
     CDS             complement(2192..3211)
                     /locus_tag="PLF77_08125"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08859.1"
                     /translation="MSRVSKKLKIGLTYDMRDDYLKEGYGLEETAEFDLPETIEAIER
                     VILDNGFQADRIGNIKALSRRLVAGDRWDLVFNIAEGMYGFGREAQVPALLEAYNIPY
                     TFSDPLGNAITLHKGFTKQILRDAGIPTPAFVVVDTAAHIDTISLPFPLFAKPVAEGT
                     GKGITALSKITSRKNLRAVCLNLLKTFRQPVLVETYLPGREFTVGILGTGKDARALGV
                     LEVILKPEAEKNAYSYENKEHYDKLVDYVLVDDPEARKAADISLMAWRALDLRDAGRV
                     DLRSNTYDVPHFMEVNSLAGLNPVRSDLPILCSKIGMSYHELICAIIKSALQRVKNHP
                     ENRCK"
     gene            complement(3250..4557)
                     /locus_tag="PLF77_08130"
     CDS             complement(3250..4557)
                     /locus_tag="PLF77_08130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPC08860.1"
                     /translation="MESETLFKEEEEPPGHLTGLVHVKNNVSDFLLFKDEVLPYPVKQ
                     KSENVLFPFKDSARSRLFRSQFYPEITRFEWFDWRWQLRNTIRDADRLAQIIHLSDNE
                     HQAMAHHSGALPVSITPYYASLLNPDDPADALRRCVVPVVDECLHTEGEEEDPLCEDA
                     DSPVPGIIHRYPDRVLFLVTDICSTYCRYCTRSRIIERSTHHLINLEKWENAIQYIAG
                     NKNIRDVLLSGGDPLTLSDDKLEWLLARLHKIRHVELIRIGTKVPVVLPQRITPRLTA
                     MLKKYHPLWMSIHITHPRELTPEMSAACIRLANAGIPLGSQTVLLSGINDDVATMTRL
                     VQGQLQIRVRPYYLYQCDPIPGSSHFRTPISKGIEIIQGMRGHTTGYAVPTYVVDAPG
                     GGGKIPLLPEYAIGWENGSLLLRNYEGNIFRYPDNHAKSKDMH"
     gene            5073..5846
                     /gene="mutM"
                     /locus_tag="PLF77_08135"
     CDS             5073..5846
                     /gene="mutM"
                     /locus_tag="PLF77_08135"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HPC08861.1"
                     /translation="MPELPEVETLCRQLQKKIAGRSITGGKVYDDKLSAVQSFKKGKA
                     INVQRQGKTIVLELDNGRAINIHLRMTGRLFWREVSLRPDYARWRLSFDKGYLYLVDP
                     RRFATVTIGDIKKDTIKNDLMKDFDEKEFMSRQAKRSVSIKTLLMDPKAVAGIGNIYA
                     SEILYASGIHPFRKASDLSEGEWEKIITSSRQILKKGIEKRGTSISDWRDIYGRPGDN
                     QHALKVYGREGTHCSKCGGEICRIKQAGRSTFYCPDCQK"
     gene            complement(5859..6368)
                     /locus_tag="PLF77_08140"
     CDS             complement(5859..6368)
                     /locus_tag="PLF77_08140"
                     /inference="COORDINATES: protein motif:HMM:NF024590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HPC08862.1"
                     /translation="MAENNISQIRINGRLVGIADLDHAIAKAARLDSKDDIDIKKCLL
                     NEISVNNYIPPSAQNAYADALLREFKIAHGLPVAEELFEGMNIEVVGMGCARCNQLEI
                     DVRDLLSEMNITAGLRHVTDFKEIARYNLLGSPALVINNKVVSVGDVPPKSKIRQWII
                     EAHQRTGKD"
     gene            6557..7150
                     /locus_tag="PLF77_08145"
     CDS             6557..7150
                     /locus_tag="PLF77_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC08863.1"
                     /translation="MDKPLMPQKLKQRLYPVVLRQFAGKDFHAVNIRDISKESGISTG
                     TIYKYFLSKEDLLFSIIDEKLLDLASLLRMHLEGTEDIREMFRKLFWVTMNFFDNNPD
                     LAVTAFITVPSKSFMASKSYKRESEIDILNGVVEKAKKRGAVISEIENRYFADLYMMI
                     TQRHIQNWYVHGMKWKLADTINQFFDFFWRVVKPIST"
     gene            7199..9388
                     /locus_tag="PLF77_08150"
     CDS             7199..9388
                     /locus_tag="PLF77_08150"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPC08864.1"
                     /translation="MVKIDILEKAKSEKRNTLTEAEAKEVLKQYGIPVVQEKAVKTIA
                     EAETESATIGYPVVVKGLGAKLTHKTEKGLVKLNLNTREDVRNACIYIKEAAGDDLEG
                     FLIQPILGGKREFVAGLFRDEQFGPTVMFGLGGIFTEALGDVVFRIVPFDEKEALKMI
                     DELNAQKLLGAFRGENAPDKKALVKTLVGLSRIGLEIPEIKEIDINPLLVSADGKVTA
                     VDALIVLGKAAERKKMVRQVDLKHIWKLFHPRSIAFVGASSKIGKWGHIMYAGVVAGK
                     YQGAIYLVNATGGEIAGRKVYSSVAEIPESVDLAVVTVPADKILSLIPQFKEKGINNV
                     LIITSGFSEVGSEGKILEEELVKKAREAGIVIVGPNTMGICNPYISFYCIGSHVRPPA
                     GGTSLLAQSGNLGTQLLAFAAKEDIGIRAYCGSGNEAMLTIEDYMDGFEVDEKTKNIV
                     LYIESIKNGQRFVETARRVGKHKPVIVLKGGRTKAGSSAAASHTGAMASDIKVFTAAC
                     KQAGIIQAESPMDLLDLSAAFSSLPLPKGSSVALLTLGGGWGVVASDLCEENNLIVPK
                     LSEDIIAEINPLLPPFWSHANPIDVVGDMNSETYMKILESLSRWSGCDAIIHMGIIGR
                     KVMIRSIMESTAAVDNKYDSNFVEGSLQYIEAFEKRTIEQTVQLMEKYNKPIVGVYLL
                     SDDQTRTVTNVNGCKYKGVNFVTPEKAVKALAKMYQYSKWLKCENEN"
     gene            complement(9492..10952)
                     /locus_tag="PLF77_08155"
     CDS             complement(9492..10952)
                     /locus_tag="PLF77_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HPC08865.1"
                     /translation="MENKNRKTFFMFLMAFLIAFFIVSFVEVLRSSLAPSGAPEIPAA
                     SALEGTTPGSFSDLTERVKPAVVNISTTKTYKGRTLGNPFGRSPFNDYFGDDFFNRFF
                     GDIPQQDLKQRSLGSGFIISNDGYIFTNNHVVENTDKILVKISDGKEYEAKIIGTDAK
                     TDIALIKIKPDNGLPTVEIGDSDAVRVGEWVIAIGNPFGLEQTVTAGIVSAKGRVIGA
                     GAYDNFIQTDASINPGNSGGPLFNMQGKVIGINTAIVAQGQGIGFAIPINMAKNILND
                     LKTKGKVTRGWLGVSIQNISEDIAKSMNYKGKSGVLVSDVFKGDPADRAGIKVGDIIV
                     EINGKSIKDTHELLLTTAGLHVGEKAIIKVIRDGREMSFSIIVAERKDKPETALTQKS
                     GGYFGIHAQEITKDMAKQLGIAANTGVIVTDVEGGSPADNVGIQTNDVIIQVNKIKII
                     SLKQYLAEISKAAEKKNVVLLVKRGNVNFFVGLSVE"
     gene            11340..11427
                     /locus_tag="PLF77_08160"
     tRNA            11340..11427
                     /locus_tag="PLF77_08160"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11374..11376,aa:Leu,seq:taa)
BASE COUNT         3410 a         2452 c         2783 g         3240 t
ORIGIN      
        1 ggaatgtctt gtgcattttt tcgtatgttt tatgggtgat gccgtcggct tcgtgttcga
       61 tttccttgag tttggcgact cgtacatcgg agtaatcccg tgcctgagcc atttccaaaa
      121 acagcttccc gccttcttcg attttgtcgg ccaactcgtc aaaaagttca aagaaatttt
      181 cttctttggg aaacaggcgc atgaaaaaat cctcctagcg gtccttgtta ttaatgttgt
      241 cgaaaagcca ttaattactg gcagtgctct agcgaattat cgggaaaaat gcaaggatta
      301 aaacgacaaa aaagacgtaa aagtgaatta aatcctgtta tttaagtacc ttagcgtaaa
      361 taatttttcc gtccccgtcc gagtaaaaat tcttcagaaa agcttccttg gcataaccgc
      421 aggactcata aaatttatgg gtgggcgtgt attgtgcccg cgaggaggtc tccgcgtata
      481 tttgcctgcc gccgttttga taaatgcggt tttctgtttc ggccatcagc atttttccga
      541 ctcccagccc ctgtaaatct ttggcaacga cgatccagta aagatcgtag ctcccggccg
      601 tggccggtat gaggccgtag cacgtatagc cgacgacctg gttttcaatt tcggcaaata
      661 aaaactggta agaactgacc tcctgtttgt ccagtttatc ttcagccagt tcgatggcta
      721 tttcgatttc ttcatcagaa aagaagccgc tggattccac catatcggca atgacttcga
      781 tgtcggaagg tttaatttgc tggcgataga taaatttttt catattttgc atcacgtatg
      841 cgggaggcct tcacgaaaca tgaagtaaac cgctccgacg aggcaaaggc cggcccacag
      901 aaaatccatc ttcaacggtt gtttcatata catgatcgca aaaccggcaa acacaatcat
      961 ggtaatcact tcctgaatca ctttaagctg tcccaggctg taataatgaa aaccgatacg
     1021 attggcggga acctgtaaaa catattcaaa aaaggcgacg ccccaactga acaagatgac
     1081 aaacaataaa ggcttgtggt tgaagttttt cagatgtccg taccaggcaa acgtcatgaa
     1141 taagttggaa aagaaaagga atatgatggt ttttaacaca gatattagat ctccttggat
     1201 atagatttca acgcgttatc aataattaaa ccgatagcct cgtgatattt aatttcagcc
     1261 ctctccagcg ccgcagcaaa accggcatcc ggcgacaaac acggattggt gttgacctcc
     1321 aatacccagg gcttgttgtt cttgtcaaca cgaaagtcaa cacgcgcgta cccgcgcaac
     1381 gaaaaaatat tccagcactg cagggcaatg tcctgtaagg acgatatgag gagggcatcc
     1441 tcgtcatcaa aattgaattt tcgcactgta ttattgtatt caaaggaatc cgcgtcccat
     1501 ttggccttgt aatcaacaat tttcaattta tccggagcat aatcctgaaa cagcatttcc
     1561 gctatcggca aaactctggc cccggcctga tcagacatta aagcaacgtt gaactcgcgg
     1621 ccgtcgatgt aggcttcggc gtaacaagag ccgcccaatt ttttttcgcg gagtttcatt
     1681 tctttgatga tcgcagtttt gtccgtatag gaaatcagtg agttttcatc aagaccgatc
     1741 gaggcatgtt cccaggacga cttgattaaa aattttccgc catcatcctg tcggaagatg
     1801 atgccattct gttccagcca atccggcgtg gcaatgccgg catcgcgcat gatttttttg
     1861 gccgtcggct tgttggacgt gaggtatatg gcgtgtgtcg ggcaaccggt ataaggaatc
     1921 tgcaacacat caagaacagc gggagcgaaa tgaatcaggc tgcctttgga gtccagggtt
     1981 tctacaatat taaaaacgac cgccggtttc aatgattgca gcatggttat ggtacgattc
     2041 aggttgagtt caaaaggcag caaaaccggt tcataatgca gcgtgcgcac ggcgtcggca
     2101 atggcttcgg cctgcacgag acaatccagt tcgtccaggc tgccatccgt tgcgatatca
     2161 ctgtgcagaa taactacttt tttcataaaa attatttaca ccggttttca ggatgatttt
     2221 ttacacgttg gagcgccgat ttaatgatgg cgcagatcag ctcatgatag ctcatgccaa
     2281 ttttagaaca caggatgggc aaatccgaac gcaccgggtt gagaccggcc agcgaattga
     2341 cttccataaa atggggaaca tcgtacgtgt tggagcgaag gtcaacacga ccggcatctc
     2401 ttaaatccaa tgccctccag gccattaatg aaatgtccgc agcctttcgg gcctctggat
     2461 catcgaccag gacatagtca accagtttgt cgtagtgttc tttgttttcg taggagtagg
     2521 cgtttttttc ggcttccggt tttaaaataa cttccagaac gcccaaggcg cgggcgtctt
     2581 tgcctgttcc caggataccg actgtgaatt cgcgtcccgg cagatacgtt tccaccagca
     2641 caggctgccg gaaagtcttt aaaagattca ggcagacggc acgcaggttt tttcggctgg
     2701 tgattttgga tagggctgtg atgccctttc ccgttccttc cgccaccggt ttggcaaaaa
     2761 gcggaaaggg aagggagatt gtgtcgatgt gagcggctgt gtcgaccacg acaaaagcgg
     2821 gagtgggaat gccggcatca cggagaatct gtttggtaaa gcccttatgc agcgtgatgg
     2881 cgttgcccag aggatcggaa aaggtgtagg ggatgttgta ggcttccaaa agagcgggga
     2941 cctgtgcttc ccggccgaaa ccgtacatgc cttcggcaat gttgaaaacc agatcccagc
     3001 ggtctcccgc aacaagcctt cgggacagag ctttaatatt gccgatccgg tcggcctgaa
     3061 aaccgttgtc cagaatgacg cgttcaatgg cttcgatggt ttcgggaaga tcgaattcag
     3121 ccgtttcttc gagaccgtat ccttccttca ggtaatcgtc gcgcatatca taggtgagac
     3181 cgattttgag tttcttgctg acgcgactca cggttgaatt ttataccccc tgtcgtcaag
     3241 actgcctgtt tagtgcatat ctttcgattt ggcgtggttg tcgggatagc gaaagatatt
     3301 gccttcataa ttacgaagca acagggagcc gttttcccac ccgatggcgt attcgggcag
     3361 caaaggaatc ttgccgccgc cgccgggtgc gtcaacaaca taagtcggta cggcgtagcc
     3421 ggtggtatgt ccgcgcatgc cctggatgat ctcgatgcct ttgctgatcg gagtccggaa
     3481 atgggaagag ccggggatgg gatcgcattg atacagataa taaggacgga ctctgatttg
     3541 cagttgaccc tgtaccagtc tggtcatggt ggcaacatca tcgttgatgc cggagagcag
     3601 aacggtctgg cttccgagcg gaataccggc attggccaga cggatgcagg cagcgctcat
     3661 ctccggcgtc agctctctgg gatgggtaat atgaatgctc atccatagcg gatggtattt
     3721 cttcagcatg gccgtcagac ggggagtgat cctttgcgga agcacaacgg gaactttggt
     3781 gccgatcctg atcagttcga cgtggcgaat cttgtgaaga cgtgccagca gccattcaag
     3841 cttgtcgtcg gacagggtta aggggtcgcc gccggagagc aacacatccc ggatgttctt
     3901 attaccggcg atatactgaa tggcgttttc ccatttttca aggttgatga gatgatgcgt
     3961 gctgcgttcg ataatgcggg aacgtgtgca gtagcggcag taggtggagc agatgtcggt
     4021 cactaaaaac aaaacgcgat ccggataacg gtgaatgatt cccggaaccg gcgaatccgc
     4081 gtcttcacag agaggatctt cctcttcccc ctccgtatgc aggcactcat ccaccaccgg
     4141 aacaacacat cggcgcagag cgtcggcagg atcatcggga ttcagcagac tggcatagta
     4201 aggcgtaatg gaaacaggca gggcgccgga atgatgcgcc atggcctggt gttcgttatc
     4261 ggagagatgg atgatctgcg cgagcctgtc ggcatcccgg atggtgttgc gcagttgcca
     4321 gcgccagtcg aaccattcaa aacgggtaat ctcgggataa aactgtgatc ggaagaggcg
     4381 ggagcgggca gaatctttaa aaggaaataa aacattctct gacttttgtt ttaccggata
     4441 aggaagaacc tcatctttga ataacaaaaa gtcactgaca ttgtttttaa catgaacaag
     4501 accggtcaaa tgaccaggag gttcttcctc ctctttaaac agggtctcgc tttccatttg
     4561 tgcctccttg atgaggacgc ccctccctta aaaaaaattt taaaataaaa atctctttga
     4621 ttatgaaatg tactcataca aaaatatttg tcaacatttt tttgcaattt tcaaaaaaaa
     4681 ttttaaaaaa gttttcagac gaaacacgtt cagaggaaaa gcttccgcat gatggcgaca
     4741 ggttttgaag gaactgccct cgaaaagcgg ttggatgtcc agaacgatga gaaaggttga
     4801 atactgatct ggaggccgtc aaatcaggct cagcgcagga taggcaattt ataaaaacat
     4861 ccacgatcag ctcgcatgca gcaaagacga ttatcagaaa tggcgtgttt cataaaaatc
     4921 gttcagccat gcgcacagga gggaggggtt gcctgacagg gcgatctgtg tcatggtgca
     4981 gcagaaaacg aataaaaaaa tattcctgcg acgcagaatc cattggtctg tccggatatg
     5041 aaagtaatga atgttcaaaa aggagacgag gtatgcccga attacccgaa gtagagacgc
     5101 tctgccggca gcttcagaaa aagattgcgg gcaggagcat taccggcggt aaagtgtatg
     5161 acgataaatt gtccgctgtc caatcgttta aaaaaggaaa ggcgataaac gttcaacggc
     5221 agggcaagac cattgttcta gagctcgata atggccgtgc gataaatatc catttgcgca
     5281 tgacggggcg attgttctgg cgggaggttt cgctgcgacc ggattatgcc cgctggcgat
     5341 tgtcgttcga taaagggtat ctgtatctgg ttgacccgcg acggttcgcc acggtaacga
     5401 tcggcgatat aaaaaaggat acaataaaaa acgatctgat gaaagatttt gatgaaaagg
     5461 agtttatgag cagacaggcg aaacggagcg tttctatcaa aacgctgctt atggatccga
     5521 aggcggttgc cggtatcggc aatatttacg cctctgaaat tttatatgcc tccggcattc
     5581 atcctttcag gaaggcgtcg gatttgtcag aaggggaatg ggaaaaaatt ataacgtcgt
     5641 ctcggcaaat tttaaagaag ggaattgaaa agagaggcac gtcgatttca gattggcggg
     5701 atatttatgg tcgcccgggt gacaatcagc atgcattgaa agtctacggt cgggaaggga
     5761 cacactgttc aaaatgcggg ggagagattt gccggatcaa acaggcgggg cgaagcactt
     5821 tttattgtcc tgattgccag aaataaagaa agattagtct aatctttgcc ggtgcgctgg
     5881 tgcgcttcga taatccattg acggattttg cttttcggag gaacgtcgcc gactgaaaca
     5941 accttattat taatgaccaa agcgggggag ccgagaagat tgtatcgcgc aatttcctta
     6001 aaatcggtga cgtgcctgag gcctgcggta atgttcatct cggacaataa atcgcgcaca
     6061 tcaatttcca attgattgca gcgtgcacat cccataccca ccacctcgat attcatcccc
     6121 tcaaatagtt cctcggcaac gggcaacccg tgggcaatct taaattctct caacagcgca
     6181 tcggcataag cgttttgtgc tgaaggagga atataattgt tcacggatat ttcattaaga
     6241 agacattttt taatgtcgat atcatccttg ctgtcgagcc gggcggcctt tgctatcgca
     6301 tgatccaaat ccgctatgcc aacaagacgg ccattgatcc tgatttgcga aatattattt
     6361 tctgccatga gcagcgtccc agttttttat aaatttattt gttcgcaaag acaacgtcaa
     6421 gcaggaaatg tattttttgg acaaatatta acattatcat attaaaataa aacaatgttt
     6481 taaaaaatac ttgacaaatg aaacaatgtt ttatattttg ccggtattca aaacatatat
     6541 ataaggaagg gtggtaatgg ataaaccttt aatgccacag aaactgaagc agcggcttta
     6601 tcctgttgta ttgaggcagt ttgccggaaa agattttcat gccgttaata ttagagacat
     6661 ctcaaaggaa agcggaatca gcacaggaac aatttataaa tattttttat cgaaagaaga
     6721 tcttttgttt tccatcattg atgaaaaatt attggacctg gcgtccttat taaggatgca
     6781 tcttgaggga acggaggata tccgtgaaat gtttcgaaaa ttgttctggg tgacgatgaa
     6841 tttcttcgat aataatccgg atctggcagt tactgctttt ataacggttc cgtcaaaaag
     6901 tttcatggcc agcaaatcat ataaacgcga atcggaaata gacatcttga atggtgttgt
     6961 ggagaaagca aaaaagcgag gtgctgttat atcggaaatt gaaaaccgtt attttgcaga
     7021 tctttatatg atgattacgc aaagacacat acaaaactgg tatgtacacg gtatgaaatg
     7081 gaaacttgcg gatacgataa atcagttctt tgatttcttc tggagagtcg taaaaccgat
     7141 aagtacataa aaatattaat aaataataaa atcagcatgc ggtaatacgg agtcaataat
     7201 ggtgaaaatc gatatcctcg aaaaagcaaa atcagaaaaa agaaatacat taacagaagc
     7261 ggaagccaaa gaagtattaa aacaatatgg tatcccggtt gttcaagaaa aagcggttaa
     7321 aacaattgca gaagcagaaa ccgaatcggc aacaatagga tatcctgttg ttgtgaaagg
     7381 attgggagca aaattaacgc ataaaacgga aaaaggtctg gttaagctga acttgaacac
     7441 gagggaagac gttcgaaatg cgtgcattta tataaaggaa gcagccggtg atgatttgga
     7501 aggattttta atccaaccca ttttgggcgg gaaaagggag tttgtcgccg gactttttcg
     7561 tgatgaacag tttggcccta ccgtcatgtt tggtttggga ggaattttta cagaagcact
     7621 cggcgatgtt gtttttcgta tagttccttt tgatgaaaaa gaagcgttga aaatgattga
     7681 tgagttaaat gcgcagaaat tgcttggagc gtttcgggga gaaaacgctc cggataaaaa
     7741 agctctagtg aaaacactgg tcggtttgtc gcgtatcggt ttagagatac cggaaatcaa
     7801 ggaaattgat attaatccat tattagtttc tgcagacgga aaagtgactg cagttgacgc
     7861 attgatcgtt ttaggaaaag ctgcggaaag aaaaaaaatg gtccgtcagg ttgatttaaa
     7921 gcatatctgg aaattatttc atccacgatc aatagcgttt gtgggtgctt cttcgaaaat
     7981 cggcaaatgg gggcatatca tgtatgccgg cgttgttgcc ggaaaatatc agggagccat
     8041 ttatcttgtt aatgccacag gaggagagat tgccggtaga aaggtttatt cgtcggttgc
     8101 ggaaattcct gagtctgtag atcttgccgt cgttaccgtt cccgctgata aaattttatc
     8161 tctgattccg caattcaagg aaaaaggcat aaacaatgtt ttaatcatca cgtcgggatt
     8221 cagtgaagta ggttcagaag gaaagatttt ggaagaagaa ctggttaaga aggcgcgaga
     8281 ggccggaata gttattgttg gtccaaacac gatgggcatt tgtaatccat atatatcatt
     8341 ttattgtata ggttcgcatg tccggccacc ggcgggcggg acctcattgc ttgcccaatc
     8401 cggtaatctg ggaacgcaat tgctggcttt tgccgcaaaa gaggatattg gaataagagc
     8461 ctattgcggt tccggaaatg aagcaatgtt gaccattgaa gactatatgg atggattcga
     8521 agttgacgag aagacaaaga acattgtctt atatattgaa agcattaaaa atggtcaaag
     8581 atttgtagaa actgccagac gtgtgggcaa acataaaccg gttattgttt taaagggcgg
     8641 acgcacaaag gccggatcca gtgctgcagc gagtcatacc ggtgcaatgg cttccgatat
     8701 taaagtgttt acagcagcgt gtaaacaggc cggcattata caagccgaaa gcccaatgga
     8761 tttactggac ctttcagcag cattttcctc attgccgttg cccaaaggca gtagtgtcgc
     8821 tcttttaacg ttgggcggag gatggggggt tgtcgcctcc gacttgtgcg aggaaaataa
     8881 tttgattgtt ccaaaattat cagaagacat cattgctgaa attaatccat tattgccgcc
     8941 cttctggagt catgccaatc ccatcgatgt tgtaggcgat atgaattcgg aaacttatat
     9001 gaaaattctg gaaagcctga gtcgatggtc cggatgtgat gccatcatcc atatggggat
     9061 tatcggcaga aaggtgatga tcagatcgat tatggagtcc actgccgcgg tggataataa
     9121 atatgattct aactttgttg aaggctcttt acagtatatc gaggcgtttg aaaaaagaac
     9181 catcgagcag acggtacaat taatggaaaa atataataag ccaattgttg gggtttattt
     9241 gttgagcgat gatcaaacca ggacagttac aaatgtaaat ggatgtaagt ataaaggggt
     9301 aaactttgtg accccggaaa aggctgtcaa ggcattggca aaaatgtatc aatattcaaa
     9361 atggctaaaa tgtgaaaatg aaaattaaca tcatggagga caagaaatgt ttcacgtgaa
     9421 acatttttgc cctgtaacaa aaaaaggcag gctttccata gcctgccttt tttaatatca
     9481 tataaattat attattctac gcttaatcct acaaagaagt tgacattgcc tcgcttcact
     9541 aaaaggacca cattcttctt ctctgcagct ttagatattt cagccagata ttgtttgagt
     9601 gaaatgattt taattttatt aacctgaata atcacatcgt tggtctgaat tccgacatta
     9661 tcagccggac tgcccccttc tacatccgta acgatgacac ccgtattggc tgcaatgcct
     9721 aattgtttgg ccatgtcttt tgtaatttcc tgggcatgaa taccgaagta cccacccgat
     9781 ttttgtgtca aagctgtttc gggtttgtct tttctttccg ccacgataat tgagaaggac
     9841 atttctcttc catcacgaat aactttgata attgcttttt ctcctacgtg cagaccggca
     9901 gtggttaaca atagctcatg agtatctttg atgctctttc cattaatttc tacaataata
     9961 tcaccaactt ttataccggc tcgatctgcc ggatcgcctt tgaaaacatc agaaacaagc
    10021 acaccgcttt tgcctttata attcatgctc tttgcaatgt cttcggaaat attctgaatc
    10081 gaaaccccta accatccgcg ggtgactttt ccttttgttt ttaaatcatt caaaatgttt
    10141 ttggccatat ttatgggaat cgcaaagcca atgccctggc cctgtgcaac aatggctgta
    10201 ttgatgccga tgactttccc ctgcatatta aataaaggtc ctccgctgtt tccgggattg
    10261 atggaggcat ccgtctgaat gaaattatcg taagcacctg cgccgatcac ccttcctttg
    10321 gcgctgacaa ttccggccgt gactgtttgt tcaagtccaa aaggattgcc gatggcaata
    10381 acccattctc ccactcttac agcatccgaa tctccgattt ccacggtcgg caatccattg
    10441 tccggtttaa tttttattaa agcaatgtcc gtctttgcgt ccgtgccgat gatttttgct
    10501 tcatattctt ttccatctga tatctttacg agaattttat cagtattttc aacaacatgg
    10561 ttatttgtga aaatgtaacc gtcattgctg ataataaaac ctgatcccag gcttctctgc
    10621 ttgaggtcct gctgcggaat gtcgccgaag aaacgattga agaagtcgtc cccaaaataa
    10681 tcattaaaag gagaacgccc aaacggatta cccagggttc ttcctttata ggtttttgtc
    10741 gtgctgatgt ttacaacagc cggtttgact ctttcggtca gatcggaaaa tgatccgggt
    10801 gtcgttcctt ccagtgccga tgcggctgga atttccggtg cgcccgaagg ggcaagagac
    10861 gatcttaaca cttctacaaa tgagacaata aaaaaggcga tcaaaaacgc catcaaaaac
    10921 atgaaaaacg ttttacggtt cttattttcc atattaatgt tataaacctc cgttattatt
    10981 ggtcaaatat tattgttgat gatagattta cgcgatatat taaatttata ctttttaaac
    11041 aatataactg caaagatgtt tttgtcaata aaatattgtt taatatgggt cagcaagaaa
    11101 catcttttca aacgttttgg gtttaaaaaa acaaacggat cattatctgc cgtcggcgaa
    11161 aatattttgc tgtataaaat tagtccatct gaagaaaatt tttttataga ttttaattgt
    11221 tagagattta aatcaaatca aaaatttcta tagatatttg aatcgtatgc cagcaaatcc
    11281 ttgacagtga atttcaaaaa taatataagc ttgacgaatt tttaaattat aaaattcatg
    11341 cctgggtggc ggaactggta gacgcaaggg acttaaaatc cctcggtcct tgaggctgtg
    11401 cgggttcaat tcccgcccca ggcaccagat cactttttaa tacccttctg ctttttgcgg
    11461 attaaaaaaa acatccttta acaacgaatt aatcacgcaa catcgcataa ccatgttttg
    11521 cagatcataa aatggtagaa aacttttttg ccggcgagcc tttttttctt tcaggaaaac
    11581 agggaataaa tcccggaatc gtagataaac tctatcgttg aattgaaaca agagttctaa
    11641 actcaaactt cagacccgtt ttgataatag cagaagaatc aaaaaccaaa caccacacag
    11701 tgaaagacca cacaataaag atgcgaatgc aatcactgac aaagcatcaa acattttttc
    11761 gatttgtcta gcgatacaat caataatgta tgagagggaa tgacgcttgt taatactcaa
    11821 acggtgttag tggtagtccc acggggactt gaaccccggt ttccggcgtg agaggccagc
    11881 gtcct
//
LOCUS       DAOOLB010000064        11879 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_5983_length_11879_cov_37.581022, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000064 DAOOLB010000000
VERSION     DAOOLB010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11879
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5983_length_11879_cov_37.581022"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="PLF77_08165"
     CDS             complement(<1..707)
                     /locus_tag="PLF77_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HPC08866.1"
                     /translation="MRKKVVLFSGESVFGSTIINRLLNKQSFDLIGVFYSTRVYKKSS
                     SIFSFYQVLSITGMQYYWNIVKLNAPFAKLFYPEVTRTGKVCRQNGIPIFYASDVHWP
                     ANIEIITQLKPDIILSAYFNQLIKEPILSFPAEVLNIHPGILPEYRGLDPLLQQIIHG
                     EDHLGMTLHRVDETFDTGEILRQKTMLRDKTKSLFELYMEIWEQGVDMLEDYILFGKQ
                     PIENLTERQQRSSYFSWP"
     gene            complement(752..1339)
                     /locus_tag="PLF77_08170"
     CDS             complement(752..1339)
                     /locus_tag="PLF77_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC08867.1"
                     /translation="MIRLSGIIQPMDMTDQNDMVDYVIEPLTFKYLLPALRLVDSVFP
                     RREQGGERADLAFTASLLTLGRRVAKWMGYPMVRYWIVRDVATNRIYGTIGHYVRADD
                     PDAYWGGWMCVDPRARGLGIGKKLINFVVGESVRRGDRPYHRFYTSTDPNEAKAVAMY
                     NRACFNVYKQECDPVTGYTRLYYQVRLDDVTLPVL"
     gene            1446..2681
                     /locus_tag="PLF77_08175"
     CDS             1446..2681
                     /locus_tag="PLF77_08175"
                     /inference="COORDINATES: protein motif:HMM:NF014511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPC08868.1"
                     /translation="MKNREIRAYLENNAGKNILPLSIIENEELIGSKPIEIIVFYKKK
                     KAISELKECLLQTIEYYNLFSSRLIKIGHHQYALQFASDGFTVNILPPVEAPVAKLPI
                     DDIRQSMVHVKTLPGEPLFAVTGFSTQDGTVGAVSCSHAVADGISFFLFLFAWVSIID
                     GNPFSRPSKQRLFTGSPLNSGHIDKMFKPSLSKLSNVIQNRIHYENNIKTYRHTDYFT
                     CEFLNDIKKHAKRGNGDDCISNNQIINAVLMKKYHQHLLPDTRKIVMRNPVNLRDVHP
                     DIDPLYIGNAYLDCTTEFTKDEVENLSVYDIARRLKESIHNAKNENFVKDMAYLSKYG
                     IEFKSDIFNHYSPDNIKTDIVSSNIAHLHDLESMGMGSNILDILHVDSAVQTGFVILK
                     ENSRLILVQTTGMYPFESR"
     gene            2987..4444
                     /locus_tag="PLF77_08180"
     CDS             2987..4444
                     /locus_tag="PLF77_08180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08869.1"
                     /translation="MRPVVLLRPAEGLYAKVFRPWVPLSLLAAASKLDEAGYPIILID
                     QRISVDWKSDLQKALSQNPICVGVTSMTGSQITHALEMSAFVKQHSAVPVIWGGVHAT
                     LFPLQTVEHSLVDIVVKGEGEETFLLLALRLEQKQSLHGLEGVVYKDRGDIVNNPDRP
                     FLDLNDLPPVPYHLIDVNKYLHRYFDEDRVLEFETSRGCPFNCTFCYNPLYSRRRWRA
                     LSASLILEFMQPLVETHGVKAFHFVDDGFFIDHERTREIMRGILNRKWKIKMGFQGAR
                     IDTVDDLQDEDLELITKAGGTFLQVGVESGSPRILDILNKKIHPEQVLAFNRRLSRHP
                     HMKVYYNFMCGFPTETAQDVLKTAALAWTLLRDNPYALISAFHFYKPYPGTSLRERVI
                     HKDDVTPSTLEGWGNFNWTQYMGRDQDADTKALMEKIEIVSILADRKMSYQSDSAALR
                     MLAAVYRPLARFRLRHGWYSWMPEHWLRKWLGDRT"
     gene            complement(4652..5656)
                     /locus_tag="PLF77_08185"
     CDS             complement(4652..5656)
                     /locus_tag="PLF77_08185"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HPC08870.1"
                     /translation="MLVRLVKDWSWPDLMRQTPQEDGKWRDIQFTFEPVKHCDYLIML
                     NNKRDIPVSVRCPPEHVWCIMQEPYIPFSNDWMLKDLDWCSRILTHYPAGLSRAEESH
                     PAVPWHVDLNYKQLMWGRRPVKQRLLSWITSRLTVTPMQRKRMAFYEYLQSCKVPVDV
                     YGRGIRHIRDKWDGLAPYYYSLAIENDRSSDYWTEKLADCFLSWTVPVYDGCLNLEKY
                     FPPESFIRINIDDPDASLKIIAETSEPDDWRRRLPALQEARELVLKRWQFFPFIYEKI
                     KTDLRQAGPASEISIPDNRIMRLTDEFRFLMDELKQRGPLALGRQLNNKIKYFHWRNY
                     "
     gene            5692..5988
                     /locus_tag="PLF77_08190"
     CDS             5692..5988
                     /locus_tag="PLF77_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08871.1"
                     /translation="MFEDFDSVKKNKKYRFTCQGNCDNPHKTLADFPLQMCGRLGKYD
                     VFIHHHILCVTEYDCSAGNIKEQPTWHEINLYEPDPDHKGAMRTNGFKKSVQGA"
     gene            6392..7045
                     /locus_tag="PLF77_08195"
     CDS             6392..7045
                     /locus_tag="PLF77_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08872.1"
                     /translation="MARIRNTLLDNIRPLQSDWAVLLDTDIYFTEEILADMFALSPSQ
                     KGIGMLCAFGMEGLPERKGTGGITRLGMFFANGMKGFPKSTGGGWITQSHYYDTWAFL
                     DEDNRFYWPHCIFSGCGKCSAKNAKDKLNAAGVVNVNSAFGGFAIVKSQLLNHPDLRW
                     KAVPSEDSDIRGSAASERKMMLCEHIHFCKMLRKVSSLSVAIACDIPVYWDLRSYRS"
     gene            complement(7084..7218)
                     /locus_tag="PLF77_08200"
     CDS             complement(7084..7218)
                     /locus_tag="PLF77_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08873.1"
                     /translation="MVNVLIEQNQRSSLIFKKLNIVKVRQPDNDGYPKHQCQNKQYKI
                     "
     gene            7445..8923
                     /locus_tag="PLF77_08205"
     CDS             7445..8923
                     /locus_tag="PLF77_08205"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPC08874.1"
                     /translation="MENNMETDSKTIYPQDVLPEISIEELTPEMRDACDRAGWKTLVP
                     VQAKSIPYFLAGRDMMVQSRTGSGKTGAYILPILQKINAQQNAAQALILVPTRELALQ
                     VSREAEMLTRETRIRIAVVYGGVGYNAQLEAFRGGAQLVIGTPGRILDHLLKNTLSLD
                     HLRILIFDEADRMLSMGFYPDMVKIRKYIQSDKILSSMFSATFPPQVIRLADQFLHHA
                     ELLSLSGSQVHIAEIEHIYYVVPGMRKERSLVRIIEIENPSSAIIFCNTKENVHYLSV
                     VLKRFGYDADELSSDLAQSMREKVMARVRRGALRFLVATDVAARGIDIPDLSHVIQYE
                     PPEDPEAYIHRAGRTGRMGSSGVAISLVAEMEKFKLQSIARYYNINMEERPLPSEDEL
                     ERVVSERITALLEAKLRARDNLQIERMRRFLPLARSLAQAEDELSLITMLLDDYYQQS
                     LHAPPAPAAEVVDQAKGHTQTDKSKKPRSRNKNRKKRQENPS"
     gene            9194..10321
                     /locus_tag="PLF77_08210"
     CDS             9194..10321
                     /locus_tag="PLF77_08210"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HPC08875.1"
                     /translation="MQNPNFSSGPCSKRPAWSIDALKGAPVGRSHRSALGKERIVKAI
                     NDTRKVLNIPADYLVGILPASDTGAFECAMWSLLGPKPVTVLVWESFSNGWATDVNKQ
                     LKLHATVIKADYGKIPDLSKVDWKTDVVFVANGTTSGVRIPDWNWIPADREGLSICDA
                     TSYAFANEVDWSKIDVLTFSWQKCLGGEAAHGMMILSPKAVARLESYDPPWPMPKIFR
                     LKKGDKISKAIFDGDVINTPSMLCVEDYLDALDWAGKIGIDGLIKKSKANLKVVEDWV
                     AKTDWIEFLADDPKVRSNTSVCLNVIHPKVAAMGPDEKSKFLKSITSDLSKKNIAHDI
                     NSYKEAPAGYRFWCGPTVEESDLKVALAELEKVFNEKVKNL"
     gene            10435..11739
                     /locus_tag="PLF77_08215"
     CDS             10435..11739
                     /locus_tag="PLF77_08215"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPC08876.1"
                     /translation="MANVAIVGTQWGDEGKGKIVDLYTEKADIIARFQGGNNAGHTLV
                     VKGKKTILHLIPSGILHDNKICVIGNGVVFDPVVFLEELEQLRQGNLLPANTKLFISE
                     KAHLIMPYHRSIDQAREARHSGKKIGTTGRGIGPAYEDKVARTGIRVGDLYEEDLFRE
                     KLHISMEEKNFLLANFFHDKPLDKQEIAAQYLAYAEKLKPFVADVSLILDREIKKGRK
                     ILFEGAQGCHLDIDHGTYPYVTSSNTVSANASCGTGIGPNAINKVVGICKAYTTRVGE
                     GPFPTELNDQVGEHLQQVGQEFGATTGRKRRCGWLDMVLVRQAVRVSGINALAITKLD
                     VLTGLDKIKICVGYKSPSGEYTYAAPASLKALAECQPVYEEFDGWNEDILYAKEISEL
                     PANTRKYLKRLEELAEAEITLISVGPGREETIVMEDPFLNRI"
     gene            11805..11879
                     /locus_tag="PLF77_08220"
     tRNA            11805..11879
                     /locus_tag="PLF77_08220"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11837..11839,aa:Lys,seq:ttt)
BASE COUNT         3320 a         2819 c         2691 g         3049 t
ORIGIN      
        1 ggccaggaaa aatatgagct gcgctgctgt ctttcagtca gattctcaat aggttgcttg
       61 ccgaaaagga tatagtcttc cagcatatcg acaccttgtt cccaaatttc catgtaaagt
      121 tcaaaaagcg attttgtttt atcccgcagc attgttttct gtctcaatat ttctcccgta
      181 tcaaacgttt catccactct atgcaaggtc atgcccagat gatcttcacc atgtatgatc
      241 tgctgcaata acggatcaag cccccgatac tccggtaaaa tgccgggatg aatattcaac
      301 acttcagccg gaaaagaaag aatgggttct tttatcagct gattaaagta cgcggacaaa
      361 ataatatccg gcttaagttg cgtgataatt tcaatatttg cgggccaatg aacatcggag
      421 gcataaaaga ttggaatacc gttttgccgg cagactttcc cggttcgggt aacctcagga
      481 taaaacaatt ttgcaaatgg agcattcagc ttcacaatat tccagtaata ttgcattccc
      541 gttatggaaa gcacttgata aaacgagaat atactggagg attttttata tactcttgtc
      601 gaataaaata caccgattaa atcaaaggat tgcttattca gaagcctgtt gatgattgtg
      661 ctgccgaaaa cactctctcc ggaaaataaa acgacttttt ttcgcacaga ttaatttttc
      721 tccaattctc ttcaacaaca cctagcataa attacaatac cggcagagtc acgtcgtcaa
      781 ggcgcacctg ataatataac cttgtatacc ctgtcacggg atcgcattct tgcttataaa
      841 cgttaaaaca ggcacgatta tacatcgcca cggctttcgc ctcattggga tctgttgagg
      901 tgtaaaagcg gtgatacggc ctgtctcctc tgcgaaccga ctcgcccaca acaaaattga
      961 tgagtttttt cccgatgccc aatccccgcg cccggggatc aacgcacatc cagcctcccc
     1021 agtacgcatc gggatcatcc gcacgcacat aatgtcctat cgtgccgtaa atccgattcg
     1081 tcgcaacatc acgcacaatc caataccgga ccatgggata tcccatccat ttggcaacgc
     1141 gccttcctaa cgtcaggaga ctggccgtaa aggctagatc agcccgttcg ccgccctgct
     1201 ctcttctggg gaaaacagaa tcaaccagtc tgagcgccgg cagaagatat ttgaacgtca
     1261 gaggctctat aacataatca accatgtcgt tttgatccgt catgtccatg ggctgtatga
     1321 ttccggataa tcttatcatg cttcgcgcat tttctgcaaa aagaaaaccg atgacctagt
     1381 cattctattt gaaattgatt ccattttgaa ttataagcga gcaatcccat gacgcaagaa
     1441 cgttgatgaa gaatagagag atccgtgcat atcttgaaaa caacgccggc aaaaatattt
     1501 tgccgctttc aatcattgaa aatgaagaac tcattggttc aaaaccgatt gaaataatcg
     1561 ttttttacaa aaaaaagaag gccatctcgg aacttaaaga atgtctgctt cagacgattg
     1621 aatattacaa tcttttttcc agtcgcctga ttaaaattgg ccatcaccaa tatgcattgc
     1681 agttcgcctc cgacggtttc accgtgaata ttctcccgcc ggttgaagca cccgtcgcaa
     1741 agcttcctat tgatgacatt aggcaatcga tggtacatgt taagacattg cccggcgaac
     1801 ctcttttcgc cgtcacgggg ttctcaaccc aggacggcac cgttggcgcc gtaagctgtt
     1861 cccacgccgt ggccgatggg atctctttct ttctcttctt gtttgcatgg gtctcaatca
     1921 tcgacggcaa tccattctct cgaccctcaa aacaacggct gtttacggga agccccctca
     1981 attccggcca tattgacaag atgttcaagc cgtcgttatc taagttaagc aatgtaattc
     2041 aaaaccggat tcattatgaa aacaatataa aaacatatcg tcatacggac tatttcacct
     2101 gtgaatttct caacgatatt aaaaagcacg caaaacgggg aaacggggat gactgcatct
     2161 ccaacaatca gattatcaat gccgtgctaa tgaaaaaata tcatcagcat ctcctgcctg
     2221 atacccgcaa gattgttatg cggaatccgg ttaatctcag agacgtgcat ccggatattg
     2281 atccgttata tatcgggaat gcatacttgg attgcaccac cgaattcacg aaagatgaag
     2341 tcgagaatct gtcggtgtat gacattgccc gtcgcctgaa agagtcgatc cataacgcaa
     2401 aaaatgaaaa ctttgtcaaa gacatggctt atctctcaaa atacggcata gaatttaaaa
     2461 gcgacatctt caaccattac tctccggaca acattaaaac ggacatcgta tcgagcaata
     2521 tagcgcatct tcatgatctt gaatcaatgg gcatgggatc gaatattttg gatattctgc
     2581 atgtggattc agctgtacag accggctttg tcatactgaa agaaaacagc agactaatcc
     2641 ttgttcagac aaccggcatg tatccctttg agtcccgtta aataagacgg atacggccga
     2701 aagtttcacg cgtcgtcgtg accgcccgtc agtccttaac gttttcataa tcttaatcaa
     2761 cattttgcga gtcttatgcg catccgcgtt atatggcgtt tgatattaac cagccggaat
     2821 acaccggaga aaattagact gggttgcgtt gaatattgat tgacatacgg agatagcttc
     2881 tctaaaattt tcgatcgtgc ggcacaacat aaagccaact aaatactgtc cgttgaaatt
     2941 aaaataaaag gatattctta ttacaatcaa cgcaaacaag aaaaaattga gaccggttgt
     3001 tttgctgcgg cctgcagaag ggctttacgc gaaagtattt cgcccgtggg tgcctttatc
     3061 gctgctggcg gcggcctcta aactggatga agccggctac ccaatcatcc tgattgatca
     3121 gcggatttct gtagactgga aatcagacct tcaaaaagct ctgtcgcaaa atcccatctg
     3181 tgtcggcgta acgtccatga ccggttctca gatcacccat gctctggaga tgtcggcctt
     3241 cgtcaagcag cattcagcgg tgccggtgat atggggaggc gttcacgcga cgctgtttcc
     3301 gttgcagact gttgaacatt ccctggtgga tattgtggtc aaaggagaag gcgaagaaac
     3361 gttcctgcta ctggctttac gtctggaaca gaaacaatca ttgcacggtc tggaaggggt
     3421 tgtgtataaa gaccgcggcg atattgtgaa taatccggac cgtcccttcc tggatttgaa
     3481 tgacctgcct cccgtacctt accacctgat tgatgttaac aagtatctgc accgctattt
     3541 tgatgaagat cgcgtgttgg aatttgagac atcgagaggc tgccctttta actgcacgtt
     3601 ttgctataat cccttatact cccggcgccg ctggcgggcc ttgagcgctt cgctaatcct
     3661 ggagtttatg caaccccttg ttgaaacgca cggcgtgaag gcttttcact ttgtagacga
     3721 cggatttttt atcgatcatg aacgtacgcg ggaaatcatg agaggcattc tgaatcgaaa
     3781 atggaaaatt aaaatggggt ttcagggtgc ccgcattgac acggttgatg accttcagga
     3841 tgaggatctg gaactgatta caaaagccgg tggcactttt ctacaggttg gcgtcgaatc
     3901 gggatcacct cgcatactgg acattctcaa taaaaaaatt catcccgaac aggtactggc
     3961 cttcaataga cgcctgtcgc gccatcctca catgaaggtg tattacaatt tcatgtgcgg
     4021 cttcccgacg gaaaccgctc aggatgtcct gaagaccgcg gcgttggcct ggacattatt
     4081 gcgggataat ccttatgcct tgatcagcgc cttccatttc tataaaccct atcccggcac
     4141 cagcctgcga gaacgtgtca ttcacaaaga tgatgtaaca ccttcaacac tggaaggatg
     4201 gggaaacttt aactggacac aatacatggg acgcgaccag gatgcggaca cgaaagcatt
     4261 gatggaaaaa attgagatcg taagcattct ggccgatcgg aaaatgagct atcaatccga
     4321 ttccgcagcc ctgagaatgc tggcagccgt ctatcgcccc ctggcacgtt ttcgattacg
     4381 gcacggctgg tattcttgga tgcctgaaca ctggctgcgc aaatggttgg gagacaggac
     4441 ataatccatg gattcagaga agctcctttt ccaaaagctt aaaggctggg cgagccgtca
     4501 tttattaaaa taatttctgc agaaaaagag cggaaaacaa taaaaaacca tctctaaagt
     4561 ttaacaaaat atttttttct gaaacttaat cgatttactt tgaaaaaggt caggattatt
     4621 cattttctgc cccgcatcga accgtcttct tttaataatt gcgccaatga aaatacttga
     4681 ttttgttatt gagctgccgg cccagggcta acggtcctct ttgcttcaat tcatccatca
     4741 gaaaacgaaa ctcatccgtc aggcgcatga tgcgattatc cgggatggaa atttctgatg
     4801 ccggaccggc ctgccgcaga tcagttttaa ttttttcata gatgaagggg aaaaattgcc
     4861 atcgtttcag gactaattca cgcgcctcct gcaatgcagg cagacgacga cgccaatcgt
     4921 caggctctga tgtttcagcg atgattttca aactggcatc aggatcatcg atattgatac
     4981 gaataaaaga ctccggtgga aaatattttt ccagattaag gcaaccgtca taaactggca
     5041 ccgtccagct cagaaaacaa tccgccagtt tttccgtcca gtaatcactg cttcgatcat
     5101 tttcgattgc cagcgaataa taataaggag ccaagccgtc ccatttatca cggatgtggc
     5161 ggatacctct tccataaaca tccaccggga ctttacagga ttgtaaatat tcatagaagg
     5221 ccatgcgctt acgctgcatc ggcgtaaccg tcaaccggct ggtgatccat gaaagcagac
     5281 gctgcttgac cggccgcctg ccccacatta attgtttgta gtttaggtca acatgccagg
     5341 gaacggcggg atgagattcc tcggccctgc ttagccccgc ggggtaatgc gttaagattc
     5401 ttgagcacca gtccagatcc ttcagcatcc agtcattcga aaagggtata tacggctcct
     5461 gcataatgca ccagacatgc tccggcggac aacgaactga aacggggata tcccgtttat
     5521 tgttgagcat gatcagataa tcgcagtgct taacaggttc aaaggtaaat tgaatatcgc
     5581 gccatttacc gtcttcctgc ggtgtttgac gcatcaagtc aggccacgac caatctttaa
     5641 ccagacgtac cagcatggat ttcatccgtt tgaatattca taaaacaatc attgtttgaa
     5701 gatttcgatt ctgtaaaaaa gaataagaaa taccgtttca cctgtcaagg taattgcgac
     5761 aatccccata aaactttggc cgattttcca ttgcagatgt gtgggcgttt gggaaaatat
     5821 gatgttttta tccatcatca catcctctgc gtgacagaat atgactgttc tgcaggcaat
     5881 ataaaagagc agccgacatg gcatgagatc aacctttatg aaccggatcc ggatcataaa
     5941 ggcgcaatgc ggacaaacgg ttttaaaaaa tctgtacagg gcgcctaaaa tcttattgac
     6001 gcagaaaagt gcgttcctta tactttgtct tcaaaaaaag cttttttatc aattcaatcg
     6061 atgatcggac agatcgaaaa ttgacataac ccgaaaccga tcaaacgaca ggatggtcat
     6121 gcgaatcacg ttgtcagaat caggaatacc cgttcgcaaa atttccatcc tgtggttatg
     6181 cagaaacaat caggaattcc ttccacattt atttgacaca ctgatccgac ttgaaaagac
     6241 atatgactgc gatttcgaat atttcattgc tgagaacgga tcttcagacg atacccgcac
     6301 actattgact gatttcctcc acggtcgcgt cggaaagatt ataacgcctg aggacacatc
     6361 ttttctcgac cagcagccca gaaccgaaag attggccaga atcagaaata cattgcttga
     6421 taatattcgt ccccttcaat ccgattgggc ggtattgctg gacacggata tctattttac
     6481 ggaagagatt ttggccgaca tgtttgccct gtccccctcg caaaaaggga tcggaatgct
     6541 ttgtgcattc gggatggagg gactgcccga acgcaaaggg acaggaggaa ttaccagact
     6601 cgggatgttt tttgccaacg gcatgaaggg ctttccaaaa tcgacaggag gaggatggat
     6661 aacgcagtcc cattattacg acacctgggc ttttctcgat gaagataacc gtttctactg
     6721 gccccactgc atattttccg gctgcgggaa gtgttccgcc aaaaatgcaa aagacaaact
     6781 caatgctgcc ggtgtggtaa acgtcaacag cgctttcggc ggatttgcca tagtaaaaag
     6841 ccagctttta aatcatccgg atctccggtg gaaagccgtt ccatcagaag attccgatat
     6901 ccgaggaagt gctgcatctg agagaaaaat gatgttatgt gagcatattc atttctgtaa
     6961 aatgcttcga aaagtcagca gtttatcggt ggcaattgcc tgcgacatcc ctgtgtactg
     7021 ggacttgaga tcataccgtt cttgacaatc aggactatta aattgccatc aaaagatgga
     7081 aaattaaatc ttgtattgct tattctgaca ctgatgtttg ggataaccat cattgtccgg
     7141 ttgccttacc ttgacaatat tcagtttctt gaagatgagg ctgctgcgct gattctgttc
     7201 tatcaaaaca ttcaccacgg tcccttcctt gtcggtctgc agtcatccac cgggctttat
     7261 aatcccccat gtttatttat ttgctgtcac cggcagataa gcaagaacaa ctttcctctt
     7321 gcttattaca gaatagtgaa ataaacacta aatcactttt gaaattgaaa atcataagcg
     7381 ctgttacctt ttcgggagga tacgttaatg acctcgacag accttatgca atgtgagttt
     7441 gcttatggaa aataatatgg aaacggattc aaaaactatt tatcctcagg atgttcttcc
     7501 ggaaatttcc atagaagagc ttacgcccga aatgcgcgac gcctgtgatc gtgccggatg
     7561 gaaaaccttg gtacccgtgc aggccaaatc cattccttat ttcttagcgg gccgcgacat
     7621 gatggtgcag tcgcgaaccg gcagcggcaa aaccggcgcc tacatcctgc ccattttgca
     7681 gaaaatcaac gcccagcaaa atgctgccca ggctctgatt cttgtgccta cccgtgaact
     7741 cgcgcttcag gtctcccggg aagcagaaat gctgacacgg gaaacgcgta ttagaatagc
     7801 cgtggtttac gggggtgtcg gctacaacgc ccagttggag gctttccgcg gcggcgctca
     7861 actcgttatc ggcacgccgg gacgcattct tgatcatcta ttgaaaaaca cgctttcact
     7921 ggatcatctg agaattctga tatttgacga agccgaccgg atgctgtcca tgggttttta
     7981 tcccgacatg gtgaaaatcc gtaaatatat ccagtctgac aaaatattga gcagtatgtt
     8041 ttccgcaacg tttcctcctc aagtcatccg cctggccgat cagtttttac atcatgcaga
     8101 attgctgagc ctgagcggca gtcaggtcca cattgccgaa atagaacaca tttattatgt
     8161 cgtgcctggc atgcgcaagg aaagatcgct tgtccggatc atcgaaatag aaaatccttc
     8221 ttcggccatt atcttctgca acacaaaaga aaatgtccac tatctgtctg tcgtgcttaa
     8281 acgattcggt tatgatgccg atgaactcag ttccgatctg gcccaaagca tgagggaaaa
     8341 ggttatggcg cgtgtccgac ggggtgcgtt gcgttttctg gtggcaaccg acgtcgccgc
     8401 acgtggaatt gatatccccg atctgtctca cgtcattcag tacgaaccgc cggaagatcc
     8461 cgaagcctat attcaccgtg ccggacgcac cggccggatg ggatcgagcg gcgtggcgat
     8521 ttccctggtc gcagaaatgg aaaaattcaa actgcagagc atcgcccgct actacaacat
     8581 caacatggaa gagcgacccc tgccctcgga agacgagctg gaaagggttg tatctgaaag
     8641 aataaccgcg cttctggaag caaaactgcg cgcccgcgat aatctgcaga ttgaaagaat
     8701 gcggcgtttt ttgcctctgg caagatccct ggcgcaggca gaagatgaac tttcattaat
     8761 caccatgctc ctggatgatt attatcaaca atctttgcac gctccgccgg ctccggccgc
     8821 cgaggttgtt gatcaggcca agggccatac acaaacggat aaaagcaaaa aaccccgttc
     8881 aagaaataaa aacaggaaaa aaaggcaaga aaacccttcg taaagtccaa gtgatttggt
     8941 ccttccccgg attttccggc tttatgattc ccagggctta ttatttctaa atatccagat
     9001 caattaacat tttccccgag aaaaacatat cggaccggac ggtacctatt ttttgccaat
     9061 tgagctattt tggaaattct catcggcgca agatgtttcc cccggtaaaa aataagctaa
     9121 aatttcttga gtaaagattt caatgctgat ataaataatt agttttttaa aatttatctt
     9181 aaggatgttt attatgcaaa acccaaactt tagttccggt ccatgcagca aaagaccagc
     9241 gtggagtatc gacgcgctga agggcgcgcc ggttggccgc tcacaccgtt ccgcactggg
     9301 caaagaaaga atcgttaaag ccatcaatga cacacgaaaa gttctcaaca ttccggcaga
     9361 ttaccttgtg ggtattcttc cggcttcaga taccggcgct tttgaatgcg ccatgtggtc
     9421 acttctgggt cctaaaccgg ttaccgttct ggtctgggaa agtttttcca acggctgggc
     9481 aaccgacgtc aataagcaac tcaagctcca tgcaaccgtt atcaaggcag attacggcaa
     9541 gattcccgat ctttccaaag tggactggaa aacggatgtg gtttttgtcg ccaacggcac
     9601 aaccagcggc gtgaggattc ccgactggaa ctggattccg gccgaccgtg aaggtctgtc
     9661 gatttgcgac gcaaccagtt atgcctttgc caatgaggtt gactggagta aaattgacgt
     9721 tctgaccttc tcctggcaga agtgcctagg cggtgaagcc gcgcacggaa tgatgattct
     9781 tagtcccaaa gccgtcgccc gtctcgagtc ctacgatcct ccgtggccga tgcctaaaat
     9841 tttccgactg aaaaaaggcg acaaaatcag caaagcgatc tttgacggcg atgtcatcaa
     9901 cacgccttcc atgctttgcg tggaagatta cctggacgct cttgattggg caggtaaaat
     9961 cggcattgac ggtctgatca agaaaagcaa ggccaatttg aaagttgtcg aagattgggt
    10021 agccaaaacc gactggattg aatttctggc cgatgatccc aaagtacgct ccaacacgtc
    10081 tgtctgcctg aatgtgatcc atcctaaagt agcggcaatg ggtcccgatg aaaagagcaa
    10141 gttcctgaaa agcatcactt ccgatctgag taagaaaaat attgctcacg atatcaattc
    10201 atacaaggaa gcgccggcgg gttaccgttt ctggtgcggc ccgacggttg aagagtctga
    10261 tctcaaggtg gcacttgccg aactggaaaa ggtttttaac gaaaaggtta aaaaccttta
    10321 attgattgac gagatttaaa tatccatacg gcagcgggaa gctattgcat catgatactg
    10381 ccagagctgc cgtatgttat ttctatccaa cccattaaac gtaggaaata aatcatggcc
    10441 aatgttgcta tcgtaggcac tcagtgggga gacgagggca agggaaagat tgttgatctt
    10501 tacaccgaaa aagccgatat catcgcccgg tttcagggcg gcaataatgc cggccatact
    10561 ttggtggtca agggcaaaaa aacaattctg catctgatcc cctcgggaat tctgcatgat
    10621 aataaaattt gcgtcatcgg caacggcgtg gtgtttgatc cggtcgtgtt tctcgaagaa
    10681 ctggaacagt tacgacaggg caatctgtta ccggccaaca caaagctgtt tatcagtgag
    10741 aaagcccatc tcatcatgcc ctatcaccgg agtatcgatc aggcacgaga agcccgtcat
    10801 tcgggcaaaa aaataggaac gaccggccgc ggcatcggtc ccgcctatga ggataaagtt
    10861 gcccgcacgg gtatccgcgt gggtgacctc tatgaagaag atttgttcag agaaaaactg
    10921 cacataagca tggaagaaaa gaattttctt ctggccaatt tttttcatga caaaccgttg
    10981 gacaaacaag aaatcgcagc tcagtacctg gcctatgctg aaaaactcaa gccgttcgtg
    11041 gcggatgttt ccctgattct cgatcgcgaa atcaagaagg ggaggaaaat tctttttgag
    11101 ggtgctcagg gatgccatct cgatatcgat cacggaacgt atccttatgt aacatcgtcc
    11161 aacacggtct ccgccaatgc ttcatgtggg acgggaatcg gtcccaatgc gatcaataaa
    11221 gtagtcggta tctgtaaagc ctacaccacc cgtgtcgggg aaggcccttt tcccacggaa
    11281 ttgaacgatc aggttggcga gcatctgcag caggtcggac aggagttcgg cgccaccacc
    11341 gggagaaaac gccgttgcgg ctggctagac atggtcctgg tacgacaggc agtacgtgtt
    11401 tccggcataa atgctcttgc catcacaaaa ctggacgttt taaccggcct ggataaaata
    11461 aaaatatgcg tcggatataa atcgccgtcc ggcgaatata catatgccgc accggccagt
    11521 ttaaaagctc ttgctgaatg tcagccggtt tacgaagaat tcgatggctg gaatgaagac
    11581 atcctttacg ccaaggaaat aagtgaattg ccggccaaca cccgaaaata tttaaagcgg
    11641 ctggaagaac tggcagaagc ggaaattaca ctcatatcgg tcgggccggg cagagaagaa
    11701 accatcgtta tggaagatcc ttttctgaac aggatttagc agttttttct tgacacagtg
    11761 ccaactagtt gttaagaggg cacgctttta gaattgcaga caaagggctg ttagctcagc
    11821 ggtagagcag tggactttta atccattggc cgcgggttca atccccgcac agcccacca
//
LOCUS       DAOOLB010000065        11857 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_5996_length_11857_cov_48.220217, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000065 DAOOLB010000000
VERSION     DAOOLB010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11857
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5996_length_11857_cov_48.220217"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..86)
                     /locus_tag="PLF77_08225"
     tRNA            complement(10..86)
                     /locus_tag="PLF77_08225"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(50..52),aa:Arg,seq:cct)
     gene            complement(535..879)
                     /locus_tag="PLF77_08230"
     CDS             complement(535..879)
                     /locus_tag="PLF77_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08877.1"
                     /translation="MPDLLIKVALYQSNYLAAGAASGAGAASGAGAGAASGAGAGAAS
                     GAGASTAGASAGAVSSAFLQPTTANDSEATNSIATKIANIFFINREPPFKDFRDLVYG
                     PSFCMGDTLYSI"
     gene            1081..1473
                     /locus_tag="PLF77_08235"
     CDS             1081..1473
                     /locus_tag="PLF77_08235"
                     /inference="COORDINATES: protein motif:HMM:NF027575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4907 domain-containing protein"
                     /protein_id="HPC08878.1"
                     /translation="MGKRKTLLWVFSMMVISALVVVFQTELSVCKEAVNKETGQETKV
                     RRGPYADAKITFRIIPSASNTYGYDILMNGKTFIHQPNIPALPGNKGFATPDKAAKVA
                     SLVAAKIRNNDMPPVVTMDDLNRMGVLK"
     gene            1522..3081
                     /locus_tag="PLF77_08240"
     CDS             1522..3081
                     /locus_tag="PLF77_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08879.1"
                     /translation="MKKYVLVIVVLFLSFLHVGMLYAEKKADFGGAGRYAAVGFAIDG
                     KGYVGTGYDGSTAYKDFWEYDPVANVWTQKADFGGGKRYAAAGFVIDGKGYVGTGYDD
                     SINYKDFWAYDPVANVWTRKTDFGGVIRNGAVGFAVGDIGYIGTGGDGSTNYKDFWAY
                     DPMADAWTQKTEFGGEERIFAVGFSIGSKGYVGTGLNGATKFKDFWVYDPATDAWVQK
                     INAGETARYGAVGFPMDDKGYVGMGGDGATAYKDFGEYNPATDTWTPKVDFEGAARGL
                     AVGFSIGSKGYIGLGLSETAKHKDFWQYDPNLDKTPDPFTFVDQTEVDWNRTITSNTI
                     TVSGVDASVVISITGGTYSINGGDYTSEDGTVNNGDTVTVRQTSSSVFHTTTDAELTI
                     GDISDTFSVTTKYLYDAGDNGPCFIATAAFGSPMAGQVEILRWFRDNYLLTNDAGRKF
                     VAWYYRYGSLAANFIKDKPLIRATVRWALYPLIGFALLLMSGTWPFVMIGILLSTFIC
                     LRVRSGKSKPV"
     gene            3278..3718
                     /locus_tag="PLF77_08245"
     CDS             3278..3718
                     /locus_tag="PLF77_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08880.1"
                     /translation="MKKIDKTLLDIRSFEGEGYQPLIDCDQWRVAILRYCDELLPERI
                     FAVQRHNETDEVFVLLEGRCILFLGEGKDTVTSITAVDMQPRKIYNVKRGVWHSHTLD
                     RDTSVLIVENRDTTVTNSPKIILNDDHRRKIVDITRELWLEENQ"
     gene            complement(3960..4649)
                     /locus_tag="PLF77_08250"
     CDS             complement(3960..4649)
                     /locus_tag="PLF77_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPC08881.1"
                     /translation="MKKARDILIRSQLFGGLPQEYITEIEKISVDKHYNKGDIIFYDG
                     DNGVGFYLVAAGSVNVYKLSPDGKEQIFHIVKEGETIGAVPVFSGKSFPANARALSKT
                     HLLFFDRIKFIELISGKPDLTMNILALLSMRLREFTVQVENLSLKEIPGRLASYLLYL
                     AQEQGNKHFVKLNISKLQLSNILGTVPESLSRALSNMKAKKLIEEEDAGIRLLNRAEL
                     EDLAERGKTSN"
     gene            4770..6407
                     /gene="hcp"
                     /locus_tag="PLF77_08255"
                     /gene_synonym="priS"
     CDS             4770..6407
                     /gene="hcp"
                     /locus_tag="PLF77_08255"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: protein motif:HMM:NF003658.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HPC08882.1"
                     /translation="MFCYQCQETVKNQGCTVKGVCGKPDATAALQDLLIYVCKGIAVA
                     AEKLNAVDKKASRYVCEALFTTITNVAWDDGRIIDKIEEGLKIRDEVNAKAASSLSGA
                     QPDCVTWTPKSREEIMAKAMSEEVRINAASNEDVTSLRSLLIFGLKGIAAYAEHAAVL
                     GYEKDDVYAFIMEALASTTKDLSVEAMIAMVMKAGESAVNTMALLDEANTKTYGNPEI
                     TEVNIGVRNNPGILISGHDLKDMEELLRQTEGAGVDVYTHGEMLPANYYPAFKKYSHF
                     VGNYGSSWWHQNEEFETFNGPIILTTNCLIPIKKDNTYLNRLFTTSVVNYPGAKHIAD
                     RPAGGAKDFSDVIAMAKTCPPPKEIETGKIVGGFAHNQVLALADKVVTAVKSGAIKRF
                     VVMAGCDGRQKARGYFTEVAEALPKDTVILTAGCAKYRYNKLPLGDIGGIPRVLDAGQ
                     CNDSYSLAVIALKLKEVFGLKDINELPISFDIAWYEQKAVAVLLALLYLNVKGIRLGP
                     TLPAFLSPTVAKVLVEKFDIKPIGTVADDVAAMMAGK"
     gene            6508..8031
                     /locus_tag="PLF77_08260"
     CDS             6508..8031
                     /locus_tag="PLF77_08260"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HPC08883.1"
                     /translation="MSSERNEQNIVIVSRGNEVIETITLGGHMIIGRAAGADIQLEDT
                     ALSRIHAMFQKDAGRWFLVDLNSLNGTLLNGRKIAPMQPTAVNSGDIAELGEYILRFQ
                     IESQPVAPDQTRPRFVTPDIEPPPSASPLLENLVERHHEIPLWTQGDIQLKIADIIDE
                     TDDVKTFRLVGLSPVLFSFKPGQFVTIKVNINGTDVRRSYTISSSPSRPHCLEMTIKR
                     TPQGLVSNWMHDHVKLGDHLAVHGPSGLFSCFNYPSQKILFIGAGSGITPVMSMLRWI
                     VDTAADVDARLLVSAKSPREIIFKNELDWMSSRHSGIRVGITCTSRVTGADAWTGLTG
                     RCDEKMLKLFTPDILERHIFMCGPSPFMEAVKENLKLLGFNLAHLHTESFGEGRVAKG
                     EKVDARDVPKPPISAVQPVESAPAETPKAASFQVNFIKSGKSISTAGDATLLELAEAN
                     GVEIEYACRSGVCGTCKSKLNKGKVEMSENELSDEEKADGYIYPCVAHPVSDVEIEA"
     gene            8035..8772
                     /locus_tag="PLF77_08265"
     CDS             8035..8772
                     /locus_tag="PLF77_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HPC08884.1"
                     /translation="MKILRKIIEIEEELCNGCGNCVTGCAEGALQIIDGKAKVVSETF
                     CDGLGACIGECPTGALKIVEREASPFDEEAVKKHLAKKEKEPETMPCGCPSTHIQIFD
                     KPSACAEANKPKHFDNVEQESALTHWPIQIKLIPASAPFLKGGDLLILADCTAVAFPT
                     LHRDLMKGKVVMMGCPKFDDVPEYVQKFADIFKTAGIRSVTVVVMEVPCCSGMPMIVK
                     KGMEAAGKEIPMKEIVLSLRGKILKSS"
     gene            8779..9069
                     /locus_tag="PLF77_08270"
     CDS             8779..9069
                     /locus_tag="PLF77_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HPC08885.1"
                     /translation="MEQEFKTVSVMIDLYCRGHHDSRDHRCVDCEALLTYVGQRLEKC
                     PLKTNKPKCSKCPVHCYKPDMREKIKAVMRYAGPRMIYRHPILAGKHFLNKN"
     gene            complement(9078..10220)
                     /locus_tag="PLF77_08275"
     CDS             complement(9078..10220)
                     /locus_tag="PLF77_08275"
                     /inference="COORDINATES: protein motif:HMM:NF005293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPC08886.1"
                     /translation="MDVYINDIAAFLPNDPVTNDEMETVLGAVHNIPSRIKNRILKNN
                     GIEKRYYAIDRKTGELNYTNARLTAEAVRRLKPYEGFSVNDIQMLCCGTSTPDVLLPG
                     HGLMVQGELGIPSCEVITTAGICLCGITSFKTAYANVALGLSDNAVSTGSELASSLIK
                     SNFFEHLHGDRDFKNHPVLPFESDFLRWMLSDAAGAVFMSGKKNRNGLSLRVDWIENI
                     AYAGQMKTCMYAGAHKKEDGTVIGWRQLHSLEEALSSNYFALKQDTELLNREIVRVMI
                     DLGLSHTVERRKIKPDDIDWYLPHYSSDFFREKIFNAMEGIGFRIPFEKWFTNLAYKG
                     NTGSASIYVIMEELFQSGQLKSGQKLLCFIPESGRFSVAYVLLTVV"
     gene            complement(10233..11552)
                     /locus_tag="PLF77_08280"
     CDS             complement(10233..11552)
                     /locus_tag="PLF77_08280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08887.1"
                     /translation="MTKKKLKIMLINPPNCGRSIPEEEYGITSLKQIFKGEPFNLEVL
                     AGPLIDHDVMIMDLKCEPDGALWKAFDDFQPDVVGLTSLTCEANTVIGIARGVKEKSN
                     AIVVIGGNHATYNPEYFNRADFDYIAVGLAKKSFRDLIDHLAFGREPTGLAGIARCAP
                     GAPLAYTKRSFDEKDLMDDIPPRYDLVAQYRDRYFLEHLGLTMGFVITAYGCTHRCSF
                     CTIPEMTGGKYLNHSAGAVLRDIQLLGDIPFIRMVDANTFGNLSLSLDLYAKIMQSGI
                     RKKFFADVRADTIVKNPDLMKKWKEAGLYAVVIGFEDFQDARLDGYNKKYQGRVIHEA
                     IEILHDLGIIMVGDFIASPDYDESDFEKLEAFIVAHKIQIPVISVLTPIPGTPLYEKM
                     KKDIVTDDLDYYTFTNAVTKTKMPEARFYKTFADMVKRLHRQPHKTT"
BASE COUNT         3046 a         2773 c         3107 g         2931 t
ORIGIN      
        1 cgatttgatt ggtgcccctg gcgcgagtcg aacgcgcggc acacggatta ggaatccgtt
       61 gctctatcct tctgagctac aggggcaacc ttgtttcgca gaattttccg cgctttaaca
      121 tgatttgacc ggttctgtca attacccggg catgtacaaa tatcgtcggt caggaaatga
      181 taatgccggg aatgacaggg tgccactctt ttatggtata aaaaatttct caatgcatga
      241 tgtgcgggaa gctcaaggag agcggcctag cgcaggatga acggatcaat ccgaacaata
      301 aaataatgcc tgctgagagg cgatctcgag caggcattaa actggatggc ttttgagccg
      361 ttaatgatta agcacactaa cgcataaaac ggtgttgctt gtcaagacct ttacggtatt
      421 ttcttaaaaa aggatgtttg tttaaacgaa tccatatatg attttcaggg aaaccggaaa
      481 aaaggaggat cgttcatgaa aaagcctttt tatttttgac tcttgacaaa acttttaaat
      541 agagtatagc gtatctccca tacaaaaact tggtccataa accaggtccc taaaatcttt
      601 gaaaggaggt tcacggttga tgaaaaaaat atttgctatt tttgttgcta tgctgtttgt
      661 tgcctcactg tcattggcag tcgttggttg caaaaaagct gaggagacag cacctgcaga
      721 ggctccggca gttgaagcac ctgcacctga agccgctccg gcacctgcac ctgaagccgc
      781 tccggcacct gcacctgaag ctgctccggc tcctgaagcc gctcctgctg ccaaataatt
      841 gctttgatac aaagcaacct ttatcaataa atcgggcatt cattctgtga atgtccgatt
      901 tattattttt acctgttttt aaaaggcgtg acgtgtctgc aaacgtaatc atgactgccg
      961 cagatgattt ttgtgacggt caatgtttcc ttgacagtcc gggacagatt gcctatattt
     1021 catcgacttg caaaaaaaat tacagtcata aagttcattg ctgaatgagg atcgtttccg
     1081 atgggaaaaa gaaaaaccct gctttgggtt ttttccatga tggttatatc tgcactggtt
     1141 gtcgtttttc aaacggagct gtccgtttgt aaagaggctg tcaacaaaga aacgggtcag
     1201 gaaactaaag tccggagagg cccttatgct gatgcaaaaa taaccttcag gattattcct
     1261 tcggccagta atacgtatgg atatgatatt ttgatgaacg gcaaaacttt tatccatcag
     1321 ccgaatatac cggctttgcc gggaaacaaa ggatttgcaa ccccggacaa agccgcaaaa
     1381 gtggcttcat tggtggcggc taaaatcaga aacaacgata tgccgcccgt cgtgacgatg
     1441 gatgatttaa acaggatggg ggtactgaaa taacatccat caagagatga tgggtgaaga
     1501 taccagcagg agagattccg tatgaaaaaa tatgttctgg tcatcgtggt cttattctta
     1561 agttttcttc atgtcggtat gctttacgcg gaaaagaagg cggatttcgg cggagcaggc
     1621 cggtatgcag ctgtcggttt tgccatcgac ggcaaagggt atgtcggcac gggatatgat
     1681 ggttcaacgg cctataaaga tttttgggaa tatgatccgg ttgccaatgt ctggacccag
     1741 aaggcggatt tcggcggtgg aaagcggtat gcggctgcgg ggtttgttat cgacggcaag
     1801 ggatatgtcg gtacagggta tgacgattca ataaactaca aggatttttg ggcatacgat
     1861 ccggttgcta atgtctggac ccggaaaacg gatttcggcg gcgtaatcag aaatggtgcc
     1921 gtcgggtttg ccgttggcga tattggatat atcggtaccg gaggtgacgg ttcgacaaat
     1981 tacaaggatt tttgggcata cgatccgatg gccgatgcct ggacgcaaaa gactgaattt
     2041 ggaggagaag agagaatttt tgccgtcggt ttttccatcg gcagcaaagg gtacgtcggt
     2101 acaggattga atggtgcgac aaaatttaaa gatttttggg tatacgatcc ggcgaccgat
     2161 gcctgggtgc aaaagataaa tgccggcgaa acagcccggt atggtgcggt cggttttccc
     2221 atggacgata aggggtatgt cggtatggga ggtgacggcg cgacagccta taaagatttt
     2281 ggagagtaca atccggccac cgacacctgg actccaaaag ttgatttcga aggagcggca
     2341 agaggtttag ccgtcggttt ttccatcggc agcaaaggct acatcgggtt aggattgagc
     2401 gaaacggcga agcacaaaga cttttggcag tacgatccca accttgataa aacccctgat
     2461 ccgtttacgt ttgtcgatca aacggaggtt gactggaacc gcacgattac atccaacacc
     2521 attaccgtat ccggcgttga tgcaagcgtt gtcatctcca ttacaggagg cacatactcc
     2581 atcaacggcg gcgattacac cagtgaagat gggactgtca ataacgggga taccgtgacg
     2641 gtcaggcaga catcgtcttc cgtttttcat acgaccaccg atgccgaact gactatcggt
     2701 gacatctccg atactttcag tgtgaccacg aaatacctgt atgatgcagg cgacaacggg
     2761 ccctgtttta ttgcgacagc ggcattcgga tcgcccatgg ccggccaggt tgaaatcctt
     2821 cgctggttca gagacaatta tctgctgacc aatgatgccg gacgaaagtt tgttgcctgg
     2881 tattaccgct acggttctct ggcagccaat tttatcaaag ataaacctct gatcagggcg
     2941 acagtgcggt gggcacttta ccccttaatc ggatttgccc ttctgctgat gtccggcacg
     3001 tggccttttg tgatgatcgg gatacttttg tcgactttca tttgtctgcg ggtacgatca
     3061 ggcaaatcaa aaccggttta aaagatcgat ttggatcaac atgacagctc ctgaacattt
     3121 caaagttggg gagctgtttt ctttaatccg tcaacaaccc tgcctgtcaa ccggtcgtgg
     3181 atttttgata gaaccatttt ctggaatgat caaaaaacgg ctgcacgaaa acggaagata
     3241 tgctagaaaa accgatatta taaaatggag atcgtaagtg aaaaagattg ataaaacact
     3301 gctggatatt cgcagttttg aaggtgaggg ctaccagccg ctgattgatt gcgatcaatg
     3361 gcgtgtggcg attctgcgtt attgtgatga actgctgcct gaacgcattt tcgccgtgca
     3421 gcggcacaat gaaaccgatg aggtgtttgt gcttttggaa gggcgctgca tcctttttct
     3481 gggagaagga aaagatacgg tcacgtcgat tacagccgtc gatatgcagc ctcggaaaat
     3541 ttacaacgtt aagcgcggcg tctggcacag tcatacgctg gatagggata cctccgtgct
     3601 gattgtggaa aatcgcgaca caacagttac aaactcgccg aaaatcattc taaatgatga
     3661 ccatcgccgg aaaattgtgg atatcacacg cgagttgtgg cttgaagaaa atcaataatc
     3721 gtttgagcaa tgaaaaaagg acgatgtgga gaaagtccga tctaacctgc cggtaaattt
     3781 ttaagcggtt tgcaaaccgt ccgttaccgc gatgctttca tcgccgcgag gttcgttatt
     3841 ttctgaacgg ttttaaaacg atcacgcaga atgggcgctg ctgtggtcag attcatttct
     3901 caatgcgcat caacgcaggc agggatttct caaccaatgg atcttctctt gccgttcgat
     3961 taatttgagg tcttccctct ttcggccaga tcttccaact cggctcggtt gagcaatcgg
     4021 ataccggcat cttcttcctc gataagcttt ttcgccttca tattgctcaa ggctctggaa
     4081 agtgattccg gcaccgtccc cagaatgttg gaaagttgta atttggaaat gttaagtttg
     4141 acgaaatgct tgttgccctg ttcctgcgca agataaagca gataggaggc gaggcggccg
     4201 ggtatttcct tgagggacag attttccacc tggacggtga attcacgcaa gcgcatggaa
     4261 agcagcgcca gaatattcat ggtgagatcc ggcttgccgg aaatgagctc aatgaatttt
     4321 atccggtcga aaaataaaag atgcgttttg gaaagcgccc gggcattggc gggaaaagac
     4381 ttgccggaaa aaacgggtac cgcgccgatg gtttcgccct cctttacaat atggaaaatc
     4441 tgttctttgc cgtcgggaga gagtttgtaa acattgacgc taccggcggc aactagataa
     4501 aatccgacac cgttgtctcc atcataaaaa ataatatcgc ctttattgta atgtttatcg
     4561 actgaaattt tttcgatttc tgtgatatac tcttgaggca atccaccgaa aagctggctt
     4621 ctgattagaa tatcccgcgc ttttttcata ggttacgtcc ttcggagaaa aaatatatcg
     4681 ttttcttaac ataagtcaag gaaacgcgtt agttttttaa ctatactcac caacaaaaag
     4741 acaaataatc ttttacaggg aggtttcata tgttttgcta tcaatgtcag gaaacggtta
     4801 aaaatcaggg atgcacggtc aaaggtgttt gcggcaagcc cgatgcaaca gcggcccttc
     4861 aggatcttct catttatgtg tgcaagggta ttgcggtcgc cgctgaaaaa ttaaatgcgg
     4921 tggataaaaa agcaagtcgt tatgtttgcg aggcgctttt caccaccatt accaacgtag
     4981 cctgggacga tggtcgcata attgataaaa ttgaagaagg actgaagatc cgtgacgagg
     5041 tcaacgcaaa ggcggcttcc tccctgtcgg gcgcacagcc tgactgtgtc acatggacac
     5101 cgaaaagtag agaagagatt atggctaagg ccatgtcgga ggaagtgcgc atcaatgccg
     5161 cgtcgaatga ggacgtaacc tccctgcgat ccctgctcat tttcggactc aagggcattg
     5221 ctgcctacgc ggaacatgcc gccgttttgg gctatgagaa agatgatgtc tatgccttca
     5281 tcatggaagc gctggcatcc accacaaaag atctttccgt ggaggcaatg attgccatgg
     5341 tcatgaaagc cggtgagtcg gctgtgaaca ccatggcgct tctggatgag gccaatacca
     5401 aaacctacgg gaatcccgaa attacggaag tcaacattgg cgttcgcaat aatcccggca
     5461 ttctgatttc aggccacgac ctgaaggata tggaagagtt gctcaggcaa acggaaggtg
     5521 ccggcgtgga tgtgtatacg cacggcgaga tgctgccggc caactattat ccggcgttta
     5581 aaaaatacag tcatttcgtg ggcaactacg gctcgtcgtg gtggcatcag aacgaggagt
     5641 ttgaaacctt caacggtccg attattctga ccaccaattg tttgattccg ataaaaaaag
     5701 acaatacgta tttgaaccgt ctgtttacga ccagcgttgt caactatccc ggagccaaac
     5761 atatcgcgga taggcccgca ggcggcgcaa aggatttctc ggatgtcatc gctatggcga
     5821 agacctgccc cccgcccaag gaaattgaaa ccggcaagat tgtcggtgga tttgcgcata
     5881 accaggtgct ggcgctcgcc gacaaggtcg tcaccgccgt gaaatcgggt gcgatcaaac
     5941 gctttgtcgt tatggctggt tgcgatggaa gacaaaaagc tcgcggctac tttaccgaag
     6001 tcgccgaagc gctgcccaaa gacaccgtga ttctgaccgc cggctgcgcc aagtatcgct
     6061 acaacaaatt accgctgggc gatatcggcg gcattcccag agtgctcgat gcaggacagt
     6121 gcaatgactc ctactcgctg gcggtgatcg cgctgaagct gaaggaagtt ttcggcctca
     6181 aagacatcaa cgaactgccg atttcctttg atatcgcctg gtacgagcag aaggcggtgg
     6241 ccgtgctgtt ggccctgctg tatctcaacg tcaagggaat ccgcctgggc ccgacgctgc
     6301 cggccttctt gtcacccacg gttgcgaagg tgctggtgga aaaattcgat atcaaaccga
     6361 tcggcacagt cgcggatgat gttgccgcta tgatggccgg caaataatcg atggccgatc
     6421 attaaactcg gtgattctcc cggatgaaaa attcgggaga gtcaccagca gaaagagaga
     6481 atcgtgaaaa aagagaggaa ttcccctgtg tcgtcagaaa gaaacgaaca gaatatagtg
     6541 atcgtgtccc gcggcaatga agtgattgaa accataaccc tcggcggtca tatgatcatc
     6601 ggacgcgccg cgggtgcgga tattcagctt gaagataccg ccctgtcgcg cattcacgcc
     6661 atgtttcaga aggacgcggg ccgctggttt ctggtggatc tcaacagcct caacggcacg
     6721 ttgctgaacg gccggaagat cgcccctatg cagccgacag cggttaattc aggagatatt
     6781 gccgaattgg gtgagtatat attgagattt caaattgaat ctcaaccggt cgcgcctgac
     6841 cagacgcgtc cgcgttttgt gacgcccgac attgaaccgc ctccgtcagc cagtccgctc
     6901 ctggaaaatt tggtcgaaag acatcacgaa attcctctgt ggacgcaagg agatattcaa
     6961 ctgaagatcg ccgatatcat cgacgaaacg gatgatgtca agacatttcg actggtcggc
     7021 ttgtcgccgg ttctgttttc cttcaaaccc ggtcagttcg tcaccatcaa ggtgaacatc
     7081 aacggcacgg acgtgcgccg ttcttacacg atctcatcga gcccttcgcg tccacactgc
     7141 ctggaaatga cgatcaagcg cacaccgcag ggcctggtct cgaactggat gcacgaccat
     7201 gtgaagctcg gcgaccatct tgccgtgcac ggtccgtcgg gattgttttc gtgctttaac
     7261 tacccgtcgc aaaagattct gtttatcggt gcgggcagcg ggatcacacc ggtcatgtcc
     7321 atgctccgct ggatcgtgga cacggcggcc gatgtggacg cgcgcctgct ggtatctgcc
     7381 aagtctccgc gcgagatcat cttcaaaaac gagcttgact ggatgtcgtc gcgccacagc
     7441 ggcatccgcg tgggtatcac ctgcaccagc cgcgtcaccg gcgcggatgc atggactggt
     7501 ctgaccggcc gctgtgacga gaaaatgctg aaactgttca cgccggatat tctggagcgg
     7561 catattttca tgtgcggtcc ttcgccgttc atggaagcgg ttaaggaaaa tttaaaatta
     7621 ctcggtttta atcttgccca tctgcacacg gaaagttttg gcgaagggcg ggtggccaaa
     7681 ggggagaagg ttgatgcgcg cgacgttcca aagccgccta tctcggcagt tcagccggtt
     7741 gaaagcgcac cggcggagac gccgaaagcg gcaagctttc aggtgaactt catcaagtcg
     7801 ggaaaatcca tatcgaccgc gggagatgcg acgttgcttg aactggcgga agcgaacggt
     7861 gtggagattg aatacgcgtg ccggtccggc gtctgcggga catgcaaatc taaactgaac
     7921 aagggcaagg tagaaatgag tgaaaacgaa ctgtccgatg aggaaaaagc ggacggatac
     7981 atctacccct gtgtggcaca tccggtgtcg gatgtagaaa tagaggcata aattatgaaa
     8041 atattacgca agattatcga gattgaagaa gaattgtgta acggttgcgg caattgcgtg
     8101 accggctgcg ccgagggcgc tctgcaaatc atcgacggca aggccaaggt ggtatcggag
     8161 acgttctgcg acggcctggg tgcctgtatc ggcgaatgcc ctacgggtgc gctgaaaatt
     8221 gtcgagcgcg aagcgtctcc gtttgatgaa gaagccgtca aaaaacatct ggcaaaaaag
     8281 gaaaaagaac cggagacgat gccttgcggt tgtccgtcaa ctcacattca gatttttgac
     8341 aaaccctccg cctgcgcaga ggccaataag ccgaaacact tcgacaatgt ggaacaagaa
     8401 tccgctctca cccactggcc gattcagatc aaattaattc cggccagtgc ccccttcctg
     8461 aaaggcggcg acctgctgat ccttgcggac tgcacggccg tcgcctttcc cacccttcat
     8521 cgcgatttaa tgaagggaaa agtggtgatg atgggatgtc cgaaatttga cgatgtcccg
     8581 gagtatgtgc aaaaatttgc cgacatcttc aaaaccgccg gaatcaggag cgtcaccgtg
     8641 gtggtgatgg aggtgccctg ctgttcggga atgccgatga ttgtcaaaaa aggcatggag
     8701 gcggcaggca aggagattcc catgaaagaa atcgtgctga gcttgagggg aaagatctta
     8761 aagtcttcat gaaggacaat ggaacaggaa tttaaaaccg tcagtgtgat gattgatctt
     8821 tactgccgcg gtcatcatga cagtcgggat catcgttgcg ttgactgcga agcgttgctg
     8881 acctacgtcg gacaacgcct ggaaaaatgt ccactcaaga cgaataagcc caaatgctca
     8941 aaatgtcccg tccactgcta taaaccggac atgcgggaaa aaatcaaagc ggtgatgcgg
     9001 tatgccggtc cgcgcatgat ttatcgccat cccattctgg cgggaaaaca ttttctgaat
     9061 aaaaattgat acaggactca gaccaccgtc agcagaacat aggcgacgga aaaccggccg
     9121 ctttccggta taaagcagag aagtttctgt ccgctcttca attgacccga ctggaacaat
     9181 tcttccatga tcacgtaaat ggaagcagac cccgtgttgc ccttgtaggc caggttggta
     9241 aaccactttt caaagggaat tctgaaaccg attccttcca tggcgttaaa gattttttcc
     9301 cggaagaaat ccgatgaata atgaggaagg taccagtcta tgtcatccgg ttttattttt
     9361 cttcgttcaa cggtatggga aaggcccaga tcgatcatca cgcggacgat ttcccggttg
     9421 agcagttcgg tgtcctgctt gagagcgaaa taattactgc tcagcgcctc ttccagcgaa
     9481 tgaagctgac gccagccgat cactgtgccg tcttcttttt tgtgcgcgcc ggcgtacatg
     9541 caggttttca tctgtcccgc ataagcaatg ttttcgatcc agtccacgcg caacgaaaga
     9601 ccgtttcggt tttttttgcc ggacataaaa acggcgccgg ccgcgtcgga gagcatccag
     9661 cgcaggaaat ccgactcaaa gggcaggacg ggatgattct tgaaatcgcg atcgccatgg
     9721 agatgctcaa aaaaattcga cttgatcaag gatgatgcca gttccgagcc ggtagacacg
     9781 gcgttgtcgc tcagccccag agcgacgttg gcataagcgg tcttgaatga cgtgatgccg
     9841 cacaggcaga tgcccgcggt tgtgatgact tcgcacgacg ggatgcccag ttcgccctgc
     9901 accatcagac cgtgaccggg gagaagcacg tcgggagtgg aggttccgca acagagcatc
     9961 tggatgtcgt tgaccgaaaa gccttcatag ggtttcaatc tgcgtacggc ttcggcggtc
    10021 agccgggcat tggtgtagtt gagttcgccc gttttgcggt caatggcgta atagcgtttt
    10081 tcgatgccgt tatttttcaa aattctgttt tttatccggg agggaatatt gtgcacggcg
    10141 cccagcaccg tttccatttc gtcgttagtc accggatcgt tgggcagaaa cgcggcaatg
    10201 tcgttgatgt aaacgtccat aaagtccttt ctttaagtgg ttttgtgcgg ctgacgatgc
    10261 agtcttttga ccatatcggc aaatgtttta tagaaccggg cttccggcat ttttgttttc
    10321 gttacggcgt tcgtaaacgt atagtaatcc agatcgtcag tgacaatatc ttttttcatc
    10381 ttttcataaa gcggcgtgcc cggaatgggc gttaaaaccg aaatcaccgg aatctggatt
    10441 ttatgggcga caataaacgc ctccagtttt tcgaaatcgc tttcgtcata atcgggcgag
    10501 gcgatgaaat cgccgaccat gatgatgccc aggtcatgca gaatttcaat ggcctcatgg
    10561 atgacgcgtc cctgatattt tttgttatag ccgtcaaggc gagcgtcctg aaaatcctca
    10621 aaaccgatca ccaccgcgta aagtccggcc tctttccatt ttttcatcag atccggattt
    10681 ttgacgattg tgtcggcccg gacatcggca aaaaactttt tccggatacc ggattgcatg
    10741 atttttgcgt aaaggtcaag cgataaagac agattaccga acgtgttggc gtccaccatt
    10801 ctgatgaagg gaatatcgcc gagcaattga atatcgcgaa ggacggcccc ggcggagtgg
    10861 ttcaaatatt ttcctcccgt catttccgga atggtgcaga aagaacaccg gtgcgtgcag
    10921 ccgtaggcgg taataacaaa acccatcgtc agaccgagat gttccaggaa atagcgatct
    10981 ctgtattggg cgaccagatc gtagcggggt gggatatcat ccattaaatc cttttcatcg
    11041 aacgatcgtt ttgtataggc cagcggcgcg ccgggcgcgc atctggcaat gccggccaga
    11101 cccgttggtt cacggccgaa agccagatga tcaatcaggt cgcggaaact cttcttggcc
    11161 agcccgacgg caatgtaatc gaagtcagca cggttaaaat attccgggtt gtaggtggcg
    11221 tgatttccgc cgatcacgac gatggcattg cttttttctt ttactccgcg ggcaatgccg
    11281 ataaccgtat tggcttcgca ggtgagcgac gtcaggccga ctacatcggg ctgaaaatca
    11341 tcgaaggcct tccagagggc gccgtccggt tcgcatttca aatccataat catcacgtca
    11401 tgatcgatca gcggaccggc cagcacttcc agattaaaag gttctccctt aaaaatctgt
    11461 ttcagcgatg tgattccgta ttcttcttcg gggatgctcc tcccgcagtt gggaggattg
    11521 atcagcataa ttttcagttt ttttttagtc atcaaagccg gttgtttgcc gcgaaaattc
    11581 tggattaaac aacaccgccg caagcagcga agtatccaaa gaaacttgcg ccccaagggt
    11641 cggggaatta gacccacaat tatcccgctc ggcctatagt gacctttagg ttattcgcct
    11701 cggggataat tcgaccatca aatttcagta cacttccacc gctttgcggg atagttcgac
    11761 ctatgcttca atcttcgctt cgctcgattt tagcaagtct cactacgttt ctgaaatttg
    11821 ggtctcatta aaaaagctct cttttcaggg agagctt
//
LOCUS       DAOOLB010000066        11836 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_6021_length_11836_cov_48.175961, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000066 DAOOLB010000000
VERSION     DAOOLB010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11836
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6021_length_11836_cov_48.175961"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..1142
                     /locus_tag="PLF77_08285"
     CDS             351..1142
                     /locus_tag="PLF77_08285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPC08888.1"
                     /translation="MIVAGCDVGSLTAKALIMNEKGRLAGEITRVRLSTLDSATEVME
                     KALASAKLRFSDLDYCCSTGYGRYEIPFAQMNMSEISCHGLGAFRADRSVRTIIDIGG
                     QDCKIILIDAEGNVRDFIMNDKCAAGTGRSLEMLAKTIGVDLETLGSLAVKSKNPISI
                     TNKCSIFMELEVLNHLYARKKLKHIACGIADAVAKRVAALAKAIDVEKNICITGGVSK
                     NPGVVRQLAYHMNVSFTSLPVDPQIIGAMGAAVYAMETLKAKVGM"
     gene            1139..>1887
                     /locus_tag="PLF77_08290"
     CDS             1139..>1887
                     /locus_tag="PLF77_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HPC08889.1"
                     /translation="MITAGVDVGTRFIKVCVTDGEHLRGSACAEIGPRFDALIRSLLK
                     EARVQARAGRWEIKKIVATGFGGHLVKKAAFTLGEAACAARSAFFFDRDIRTVIDLGG
                     LFITVSTIDKNGFLETTHVNERCAAGSGKFLEMVAEAAEVPFTEISSCAAASRQPYSS
                     SSSCAVFAESEIISQVNTGMKPSDILAGVIRAIASKTASLIDGAGAKDKIVLIGGLSK
                     VSAVRDMLTGLTGRSMTTLPIDSQLVAAFGAA"
     assembly_gap    1888..1987
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1988..2391
                     /locus_tag="PLF77_08295"
     CDS             <1988..2391
                     /locus_tag="PLF77_08295"
                     /inference="COORDINATES: protein motif:HMM:NF015555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08890.1"
                     /translation="DPTGPNIQTLSNLTNRIPGYMTRSIPGKIWIRIHRDLLARKFSL
                     QSLGQCLIQAYHESIPGLKNIEVALIADNADFVGHLIPIHNRARIIYGENKKLVLEAD
                     GTISCEDLDCENCTEKPTCDTLRDVIVKRRT"
     gene            2394..3188
                     /locus_tag="PLF77_08300"
     CDS             2394..3188
                     /locus_tag="PLF77_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC08891.1"
                     /translation="MNEPIILAFLGKGGVGKTVLSALTGTILSACGKKVLFVDADPAM
                     GLTSALEVSDIKTIGAAREEIIRQARISSSSEEKEKLADIVDYLLMEALYEGQGFGMV
                     AMGQTNTLGCYCPVNNLLRSTIKSIASQYEAVIIDAEAGIEQVNRQVVESVHYPVIVT
                     DNSLRGARTAVMISSLLKKIPQLSPVKVGTVFNRVDQADENLVKMILEAGMDYYGSLR
                     PDNAISALDRQGLSIAKIAGTSPAAAGMRSVLEQIGAFGKILNVKK"
     gene            3248..4651
                     /locus_tag="PLF77_08305"
     CDS             3248..4651
                     /locus_tag="PLF77_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPC08892.1"
                     /translation="MRDVYVVAAHTIKFGKYLEKTISELSAMTVSPCLKEAGLDKKDI
                     QALWFSNSAWGEYKRQVCIRGQVALRPLGIDAIPITNLENACAGGSTAFHHAWMGVAS
                     GMYDITMALGAEKIYDQNQALVFSGFLTGLDVGNFGKTLERLGDVALSENEKKAMATH
                     IEKYKGLAKQSSGKGRKKSFKDKFMAQRDALVVAIRIGETMGYDMLKKLRKLGSGDHS
                     PFMDVYGYEARQHMKKFGTTIEHLAAIASKNHFHSSLNPNAQYQFLVSTEQVLADRLV
                     TWPLTRSMCAPIGDGAASAILCDEATVRRLGLSGQAVKVRASILGSGMARDRDGIDIG
                     ERLSRLAYEKAGVGPAEIAFAEVHDATAYGELHQCESLGFCRLGEGGRYAESGATKLG
                     GKQPVNPSGGLESRGHPIGASGLAQIHELVTQLRGAAGPRQVSGAKLAIAENGGGALG
                     VEEAAMCIHILEAPSLK"
     gene            4674..6374
                     /locus_tag="PLF77_08310"
     CDS             4674..6374
                     /locus_tag="PLF77_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPC08893.1"
                     /translation="MNRKLRKLDLGKNILKSICLFSINEIRRGSFLKNIRGVSRTEIR
                     QEMSWGELLEEKAARYKDKIFLIFEDKTFTYRQMNANANRIANYFLSSGAGQGKGVAI
                     LMDNSPSFIDIFIGIQKIGMYAVPVNTSLKGDSLLYILNHCDAEYLVIDETHLETLNK
                     IADRLDKIKTIIINPSPSSSRQYPAGYHVLDEAYKGSPANPGLGYRKDDICFITYTSG
                     TTGLPKGVVYRYRKSSVKLLSLVAVMLFKKSDVLYTGMPLFHGNALFVTVTCALHTGA
                     RVVLARKFSASRFWDDIRKYNVTTFNTIGAMIPILMKQPEKPSDRQHNVRFIISAACP
                     ADLWEKFEKRFGLTIYETYGAVDGGGKIILNLGTAPVGSIGKPPMKVKYRLVDANMKD
                     VPDGTPGELVFPVSNKKSTSSVEYYKNEKASAEKMNGDYIFTGDLIRRDTEGFLYFIG
                     RNTESMRIKGENVSAFEVEQAIQKHSNVLEAAVYAVPSELAEDDIMASITLVEGVTLK
                     ETELIEYLKENLPKFAVPRYVKIVPELPKTETHRVKKKELEALKVVTGTWDENKKAYV
                     "
     gene            6439..6888
                     /locus_tag="PLF77_08315"
     CDS             6439..6888
                     /locus_tag="PLF77_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08894.1"
                     /translation="MSKINTGFWQKMFFVGSLWNLGIGITSLLFTDFMLMMMFGKGPI
                     EDNLLAFINGTVPVTDNLQTLIFFRFFMIAVILFGIGYYWVSRDLLANRAVIWLGLVA
                     KLIIFFTFVYYYALEQAAWFPVFVLSGDFVFSIFFVAFLWKTKDGIY"
     gene            6923..7672
                     /locus_tag="PLF77_08320"
     CDS             6923..7672
                     /locus_tag="PLF77_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HPC08895.1"
                     /translation="MAKQTTDNLSPLYTYQEGMEWNPVEKVIMERRSIRNFKKEPVPD
                     NLIRRVLEAGRFAPTAGNAQPWKFIVVKDQALIAEMERATIQLSKLLMWFVDYNRNAF
                     RRIFLAPYTKFMTRLMPNKLHPIPFNLLQQIAAEKTAVYYNAPVMILLLIDKRGIGNP
                     VLDAGICGQNIVLAAHSLGLGTCWIGMMTVLMMNPLWRRKLGVKSPYELTDCIVLGWP
                     KGDYDGEVTREVQLVEWLEAGGEKRIERQGV"
     gene            7674..8024
                     /locus_tag="PLF77_08325"
     CDS             7674..8024
                     /locus_tag="PLF77_08325"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HPC08896.1"
                     /translation="MLDLSLGKKGKIPNYFKETQMTYGVVVFDPTLCTGCGICVSICP
                     ARGLMLTKRQDSAKKKIPLVIETAPGISLCMACGDCTAACPEAAIRIKRGFNAGFFFR
                     KLSQQPELTLPKKY"
     gene            8047..9438
                     /locus_tag="PLF77_08330"
     CDS             8047..9438
                     /locus_tag="PLF77_08330"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPC08897.1"
                     /translation="MKNNPKPFQYLKWSSSFLNFGYYLTTFPNLSYWALRLTRPLYVK
                     RETFFNHKFRYAFLILGGGILPPEEIKAYHYGAKAFVDNMKNALKANENGKPVVWVEW
                     ILSSEIVNAFDAFPFNPETLNVFGNLSGQDVPSLLIEEAESAGFASEYCSAIKLTAGA
                     YMLGQTPKPAAIIAGSHPCDTNVAAYQGIRHLTGAPVFFFDSPYWRDEDAFDYYEKNM
                     WRLIEFLEKTLHQKINWERLKQIVDHENKFNFYMQEICEMMRAIPSPATMITPVFAWV
                     VRELAIGDPNGTEMARRMYEVVKDRFKKGVGVVKKENIRVLWWNPPIAFFAYVFKWME
                     DEFGAVVVKDFIGDVQMPQIDTSSEKTMIRDMAKNHLYLAMARQCHGPVEFITQELEK
                     AIDEYSPDCLIFAGHAGCKHGWAVIGIIKDLLKKRGLPSLFMNMDIMDQRHATEDDIK
                     RWITEFFRSNGFV"
     gene            9435..10625
                     /locus_tag="PLF77_08335"
     CDS             9435..10625
                     /locus_tag="PLF77_08335"
                     /inference="COORDINATES: protein motif:HMM:NF017828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase family protein"
                     /protein_id="HPC08898.1"
                     /translation="MKTIIDETRDLALSTHNHYLEEARKQGKKIIGYFCSYMPEEIIH
                     AAGFVPYRMRAVESKGTTRADAFYSSINCTFVKHCFEKALKGDFAFLDGVVFVNGCDH
                     SRRMYDNWRFAKIDPPFLHMFFAPHMINEAALDYFSLECSKLKSSIEKHFHVSINDES
                     LRRSINLYNQKRRLLAQIYALRKMKDVPIKASELLGVMLAVTAVPVERAIDILSNVKN
                     YITGRVVSKPQDLRLFFSGGCIEEMDHLELIENCGAVIVADNICLGSRHFLDEVKNDG
                     NPLQDIADRYLSHLSCPRMMGDFERRFNYLLDVRDEYGIDGIIIEKLKFCDIWGGEIY
                     LHRTESKKRGLALLALERELYGGGIGQIKTRVQAFLEKLRNKQATDENLVQAAGADYK
                     PKIQ"
     gene            10644..>11836
                     /locus_tag="PLF77_08340"
     CDS             10644..>11836
                     /locus_tag="PLF77_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HPC08899.1"
                     /translation="MKKKGSGYEYIIVGTGPGGAPAARELAKAGKKVLMVEKGDYHKK
                     FLGLPFGIRLLERYMLFARSREGVIIERGITVGGSSMVYQSNVMDPPVKLINNMGVDF
                     RPEAKQLKKEIGIEVLPEKFYRNNKGLYRMLATADKMGIPFREQEKFVHADKCKLGCD
                     WCMLGCPENARWTTREHVEEAKSYGAELIFNAPVNKILFNAGKTKATGIQLKNGTVIH
                     GDNIILSAGGIGSPAVLLRSGIKRLGTQDVGTRFFMDPMNIMLGYSKDPDGGLWGIQT
                     FSHAIESMAQSEGFMIGNCSAFGTWTVLSFFRMQTARENLPKFPIMKRGMGLFLKLSD
                     EAHGQIFPNEKTSKPFTANDEKRLKKGTDIMREIIIKSGGDPKTISILKWAGGHPGGT
                     LAMGQA"
BASE COUNT         3075 a         2810 c         3074 g         2777 t
ORIGIN      
        1 ctgttttaag ccgatccgca gagaactcaa atcgactttc atctgtctgg atatttcttt
       61 tgccgaagga agctttgttc cgggtttgag cttattatta aaaatatccc tcatgagaat
      121 gcgaacaact ttctccggaa cttgttggtt ggccatatac ccttcctcct ttgtttataa
      181 ttttatacaa ttatcaatat cttgtcaaac aataattcaa ttataataat tgatatataa
      241 aattcttgac atttaatagt aatttgttat gctgatttta ctgatggtgg ataccccctc
      301 gttaattctt ttatgatttg taaagaaagg catgagtttt taagggaaca atgattgtag
      361 caggatgtga tgtcggatct cttaccgcca aggcattgat catgaatgag aagggccggc
      421 ttgcgggaga gatcacccgg gtgagattgt ctaccctgga ttcggccacg gaagtaatgg
      481 aaaaagcctt ggcatcggca aagctccgat tttccgatct ggactactgc tgcagcacag
      541 gctacggccg ttacgaaatt cctttcgccc agatgaatat gagcgagata tcctgtcacg
      601 gattgggcgc cttccgtgcg gaccgcagcg tcaggaccat tattgatatc ggaggacagg
      661 actgcaagat tatcctgatc gatgcggaag gcaatgtccg tgatttcatc atgaacgaca
      721 aatgcgccgc gggcacggga agatcattgg aaatgctggc caaaacgatc ggcgtcgacc
      781 ttgagacgtt aggaagcctg gccgttaaat cgaagaaccc catcagtatt accaacaaat
      841 gcagtatctt catggaactg gaagtcctca atcatctcta cgcaagaaaa aaacttaaac
      901 acatcgcctg cggcattgcc gatgccgttg ccaaacgggt tgcagctctg gccaaagcca
      961 ttgacgtcga gaaaaacatt tgtatcaccg gcggagtatc caagaatccc ggcgtggtga
     1021 gacagttggc ttatcatatg aacgtttcat ttacatcgct tcctgttgat ccccaaataa
     1081 tcggcgcaat gggcgccgcc gtctatgcca tggaaacgtt gaaagcgaag gtgggcatgt
     1141 gattactgcg ggagtggacg tgggaactcg atttatcaaa gtttgcgtaa ccgatggcga
     1201 gcatctgcgc gggtcagcct gcgcggagat aggtccccgg ttcgatgccc ttatccgttc
     1261 tttgttaaag gaagcgcggg ttcaagcccg ggcgggaaga tgggaaataa aaaaaattgt
     1321 cgccaccggt ttcggcggcc atttggtgaa aaaggccgcc tttacattgg gagaagctgc
     1381 ctgcgctgcc cgatcagcct tcttttttga cagggatatt agaaccgtta ttgatctggg
     1441 ggggcttttt atcacggttt ctaccattga taaaaatggt tttctggaga ccactcatgt
     1501 gaacgagaga tgcgccgccg gaagcggcaa gtttctggaa atggtcgctg aggcggcaga
     1561 ggtacctttt actgaaattt cttcctgcgc cgccgcttcc cgccaaccct actcgtcctc
     1621 cagcagttgc gccgtcttcg ccgagagcga gatcatttcg caggtcaata ccggaatgaa
     1681 accaagcgat attctggccg gcgttatccg ggccatagcc tcaaagaccg cttccctgat
     1741 cgatggcgcc ggcgcaaagg acaaaatcgt tttgatcggc ggtctttcca aggtttcggc
     1801 agttagagac atgttaacgg gacttaccgg cagaagcatg acgacgttgc caatagacag
     1861 ccagttggtt gccgcttttg gcgccgcnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnttg atcccacggg tcctaatata caaaccctct caaatctgac gaatcggatt
     2041 ccgggataca tgacccggag cattcccggc aagatatgga tcaggatcca tcgggatctc
     2101 ctggccagga aattttccct ccagtccctc ggacagtgtc ttatacaggc ctatcatgag
     2161 tctatccccg ggctgaaaaa catcgaagtc gcccttatcg ccgataatgc cgacttcgtg
     2221 gggcatctta ttccgattca taaccgcgcc cgcatcattt acggcgaaaa taaaaagctg
     2281 gttctggaag cggacggaac catcagttgc gaggatcttg actgcgaaaa ttgcacggag
     2341 aaacccacct gcgatacctt gcgggatgtt attgtaaaaa ggagaacgtg atcatgaacg
     2401 aaccgattat cctcgctttc ctcgggaagg gaggcgttgg caagacggtg ttgtccgctc
     2461 ttacgggaac gatcctttcc gcttgcggca aaaaggtcct ttttgtggat gccgatccgg
     2521 ccatgggatt gaccagcgcc ctggaggttt cggacatcaa gaccatcggc gccgcgcggg
     2581 aggaaatcat ccgtcaggca agaatttcca gttcttctga agaaaaagag aagctggccg
     2641 atatcgtcga ctatctgctc atggaagcgc tttacgaagg tcagggtttc gggatggtgg
     2701 cgatgggaca gaccaacacc ctgggatgtt attgccccgt taacaatctt ctgcgcagta
     2761 cgatcaagtc gatagcatca cagtacgaag cggtgatcat cgacgcggaa gcaggcatcg
     2821 aacaagtgaa tcgccaggtt gtggaatcgg ttcattatcc cgtcattgtt acggataact
     2881 ctctcagagg agccaggaca gcggtgatga tttccagttt actgaaaaaa attccccagc
     2941 tgtcgccggt aaaggtggga actgttttta accgtgtcga tcaggcggat gaaaatctgg
     3001 tgaaaatgat tctggaggcg ggaatggact attacggcag cttgagaccg gacaacgcca
     3061 tcagcgcact ggacaggcag ggcctttcta tcgcgaaaat tgccggcaca tcccccgctg
     3121 ccgccgggat gagatccgtt ctggagcaaa ttggagcctt tgggaaaata ttaaacgtta
     3181 aaaaataaga caaagaaaga ggacctatcc ctcaatcctg ttttaaatat attttttgga
     3241 gtagattatg cgagatgttt atgtcgttgc ggcccacaca atcaagttcg gcaaatacct
     3301 ggagaaaacg atatccgaat tatcggcaat gacggtttcc ccctgtctga aagaagccgg
     3361 attggacaag aaggatatcc aggcgctctg gttttccaat tccgcctggg gtgaatacaa
     3421 aaggcaggtc tgcatccgcg gtcaggtggc tcttcgtcct ttggggatcg atgccattcc
     3481 gatcaccaac ctggaaaacg cctgtgccgg cggttccacg gcgtttcatc atgcctggat
     3541 gggcgtggcc agcgggatgt atgacatcac gatggccctg ggtgcggaaa aaatttacga
     3601 tcagaaccag gcgctcgttt tttccggttt tctgaccggc ctggatgtcg gcaactttgg
     3661 aaagacgctg gaaagattgg gcgacgtcgc tttaagcgaa aacgaaaaga aggcgatggc
     3721 gacccatatc gaaaagtaca agggcctggc aaagcaaagc tccggcaagg gcagaaaaaa
     3781 atccttcaag gacaagttca tggcccagcg ggatgccctg gttgtcgcca ttcggatcgg
     3841 cgagaccatg ggttacgaca tgctcaagaa actgcgtaaa ttaggcagcg gcgaccattc
     3901 tccctttatg gatgtttacg gctatgaagc gcgtcagcac atgaaaaagt tcggcaccac
     3961 catcgaacat ctggccgcca tcgcttccaa aaatcatttt cattcttccc tgaaccccaa
     4021 tgcacaatac caatttttag tatcgacgga gcaggttttg gcggaccgcc tggtcacctg
     4081 gccgctgacc cgttccatgt gtgcccccat cggcgacggc gccgcttcgg cgattctctg
     4141 cgacgaagca acggtaagac gcctgggatt gtcgggccag gcggtaaaag tcagggcgtc
     4201 gattctgggt tccggcatgg ccagagatcg cgacggtatc gacatcggcg aacggctgag
     4261 tcgcctcgcc tatgaaaagg ccggcgtggg acctgccgag atagcttttg ccgaagtgca
     4321 cgatgccacc gcctacggag aacttcatca atgcgagtcg ctggggttct gccgtctggg
     4381 cgaaggagga cgatatgccg aaagcggggc gacgaaattg ggcggcaagc aacccgtgaa
     4441 tcccagcggc ggtctggaat cgcgcggcca tcccatcggg gcatccggcc ttgcacagat
     4501 ccatgaactg gtgacccagc tcaggggcgc ggccgggcca agacaggtgt ccggggcaaa
     4561 acttgcaatc gctgaaaatg gcggcggagc cctgggagtg gaagaggccg cgatgtgcat
     4621 ccatattctg gaagccccgt cattgaaata atctgtatgt aaggagcaaa gcagtgaaca
     4681 gaaaactaag aaagctggat ctgggaaaaa acattttgaa atcgatctgt ttgttttcca
     4741 tcaatgaaat caggagaggt tcctttctga aaaacattcg cggcgtttcc aggacggaaa
     4801 ttcgacagga aatgtcgtgg ggagaattac tggaagaaaa ggcggcccgt tacaaggata
     4861 aaatttttct tatctttgaa gacaaaacgt tcacctatcg tcagatgaac gcaaatgcca
     4921 accggatagc caattacttt ttatcttcag gcgcagggca gggcaagggc gtggcgattc
     4981 ttatggacaa ttcaccgagc ttcatcgata ttttcatcgg gattcagaag atcggcatgt
     5041 acgccgtccc cgtaaacaca tcgttgaagg gagacagtct cctgtatatc cttaaccatt
     5101 gtgacgccga atatctggtc atcgatgaaa cgcatctgga gaccttgaac aaaatagccg
     5161 accggttgga taagataaaa accatcatca ttaatccatc cccttcttca tcgagacaat
     5221 atccggcggg atatcatgtc cttgatgaag cctataaagg atcgcccgcg aatccggggc
     5281 ttggctaccg caaagacgat atctgtttta ttacctatac ctccgggacg accgggcttc
     5341 ccaagggcgt tgtttaccgc taccgtaaat catcggtgaa actgttgtcg ctggtcgccg
     5401 ttatgctttt caaaaaaagc gacgttctct atacgggcat gcctcttttc cacggcaatg
     5461 ccctttttgt aacagtgacc tgcgccctgc atacgggagc ccgggttgtt ctggcaagga
     5521 aattctcggc cagccgtttc tgggatgata tccgaaagta caacgttacg acctttaata
     5581 ccatcggagc catgatccct attctgatga agcagccgga aaaaccttca gaccgccagc
     5641 acaatgttcg ttttattatt tcggcggcct gtcccgctga tctgtgggaa aaatttgaga
     5701 aacggttcgg actgaccatc tatgagacct atggcgccgt tgacggaggc ggcaagatca
     5761 tcctcaacct gggcactgcg ccggtgggat cgatcggcaa gccgcccatg aaagtcaagt
     5821 accgtcttgt ggatgccaac atgaaagatg tgcccgatgg tacgccgggc gagctggtct
     5881 ttcccgtttc caataagaaa agcacatcct ccgtcgagta ctataaaaac gaaaaagcgt
     5941 cggcggaaaa aatgaacggg gattatatct tcaccgggga tttgatcaga cgcgatacag
     6001 agggcttcct gtactttatc ggcagaaaca cagagtcgat gcgcattaaa ggtgaaaatg
     6061 tatcggcttt cgaggtggag caggcgatcc agaaacattc caatgttctg gaggcggctg
     6121 tttacgccgt tccgtcagaa ctggcggaag atgatatcat ggcctccatc actctggtgg
     6181 aaggcgtgac gctgaaagaa accgagttga tcgagtatct gaaggaaaac ctgcccaagt
     6241 tcgccgtgcc ccgatatgtg aagatcgttc cggaattgcc gaaaactgaa acgcaccggg
     6301 tcaagaaaaa ggaattagag gcgctgaagg tcgtcaccgg cacctgggac gaaaacaaaa
     6361 aagcctatgt gtgacggttt ctcgatgcgt ctgattataa aaattaccat tatctagtaa
     6421 caaaggaggt caaaaactat gagcaagatc aatacgggtt tctggcagaa gatgtttttt
     6481 gtcggttcgc tctggaatct gggtatcggc ataaccagtc tgttgtttac ggattttatg
     6541 ctcatgatga tgttcggaaa ggggccgata gaagacaatc tcctggcctt tatcaatggt
     6601 acggtgccgg taacggacaa cctccagacc ttgatatttt tcaggttctt tatgatcgct
     6661 gtcatcctct tcggcattgg ttactactgg gtctcgcgcg atctcctggc caaccgggcc
     6721 gtgatctggc tggggcttgt cgccaagctc atcatctttt tcaccttcgt ctactactac
     6781 gccctggagc aagcggcctg gtttcccgtc ttcgtcttat cgggcgattt cgtattctcg
     6841 atcttttttg tagccttcct ctggaagacc aaggacggca tttattgacc ctttaacccc
     6901 acaaggagag aagaggtata ccatggcaaa acaaacaacc gacaatcttt ccccgctcta
     6961 cacctaccag gagggcatgg aatggaatcc cgtggaaaag gtgatcatgg aacggcgaag
     7021 catcaggaac ttcaagaagg aaccggtgcc cgacaacctg atccgacggg tgctggaggc
     7081 gggaagattc gcccccacgg cgggaaacgc ccaaccctgg aagtttatcg tcgtcaagga
     7141 ccaggcgctg attgcagaaa tggagcgggc aacgatacaa cttagtaaat tactcatgtg
     7201 gttcgtggat tataatagaa acgccttccg gcgcatattt ctggcgccct atacgaagtt
     7261 tatgacccgg ctgatgccca acaaactcca tcccatcccc ttcaacctgc tccagcagat
     7321 cgccgccgaa aagacagccg tgtactataa cgcccccgtt atgattctcc ttttgatcga
     7381 taagcggggg atcggcaatc cggtgctcga tgccggcatc tgcggtcaaa acatcgttct
     7441 ggccgctcac agcctggggt tgggaacatg ctggatcggc atgatgacgg ttttgatgat
     7501 gaatcccctg tggcggagaa aattgggagt taaatccccc tatgagctga ccgactgtat
     7561 tgttttgggc tggccgaaag gcgactatga cggcgaggtg accagggaag tgcagctcgt
     7621 cgaatggctt gaggccggcg gggagaaacg aatcgaaagg cagggggtgt gatatgttag
     7681 atctgtcatt aggaaagaag ggaaagattc ccaattactt taaagagact cagatgacct
     7741 acggggttgt cgtcttcgat cctacgctat gtaccggctg cggcatctgc gtttccatct
     7801 gtcccgcccg gggcctgatg cttaccaaaa ggcaggacag tgccaaaaag aagataccgc
     7861 tggtgatcga aaccgcaccg gggatatcgc tttgtatggc ctgcggcgac tgcacagcgg
     7921 cctgtccgga ggcggccatc cgcatcaaac gcggattcaa cgccgggttc ttcttccgca
     7981 agttatcaca gcagccggag ttaactttgc cgaaaaaata ttaataaaat cagaaggaga
     8041 gtttcattga aaaataaccc caagccgttt caatatctta aatggagctc cagttttctg
     8101 aatttcggat attaccttac aacttttccg aatttgagtt actgggctct cagacttacc
     8161 cggcctctct atgtcaaacg cgaaacattc ttcaatcaca aattcagata tgctttcctg
     8221 attctgggag gaggaattct tccgcccgag gaaatcaaag cctaccacta tggggcaaaa
     8281 gcctttgttg ataatatgaa aaatgctcta aaagctaacg aaaacggtaa gcccgtcgtc
     8341 tgggtggaat ggattctttc ctccgaaatc gtaaatgctt ttgacgcgtt tccgttcaat
     8401 ccggaaacgc ttaatgtgtt cggaaacttg tccggacagg acgttccgtc gctgctcata
     8461 gaggaagcgg aaagcgcggg ttttgcctca gaatactgct cggccattaa gctgacggcg
     8521 ggagcctaca tgttggggca aactcccaaa cccgcggcaa tcattgccgg gtcgcatccc
     8581 tgcgacacca acgttgccgc ctatcaggga atacgccatt tgacgggtgc ccctgtgttc
     8641 tttttcgact ccccttattg gcgcgatgaa gacgccttcg attattatga aaaaaatatg
     8701 tggcgcctca tagagttcct ggaaaaaact cttcaccaga aaatcaactg ggagaggctc
     8761 aaacagattg tagatcatga aaacaaattc aacttttata tgcaggaaat ttgcgaaatg
     8821 atgcgggcaa tcccctctcc cgcaacgatg atcacgccgg tttttgcctg ggtcgtgcgg
     8881 gaactcgcca tcggcgatcc taacggcacc gaaatggcca gacgcatgta cgaggtcgta
     8941 aaggatcgct ttaaaaaagg tgtcggtgtc gttaaaaagg aaaatatccg cgttttgtgg
     9001 tggaacccgc cgattgcttt ttttgcctac gtcttcaagt ggatggaaga tgaattcggc
     9061 gccgtcgtcg tcaaggattt tatcggcgat gtccagatgc cgcagatcga tacgtcctcg
     9121 gaaaaaacaa tgattcgtga catggctaaa aatcatcttt acctggcgat ggccagacaa
     9181 tgtcacggac cggtggaatt tattacgcaa gagcttgaga aggccatcga cgagtactcg
     9241 cccgattgcc tgatcttcgc gggacacgcc ggctgtaaac acggctgggc agtcattggt
     9301 attatcaaag acctgctgaa aaaaaggggc ttgccgtcgc tgttcatgaa tatggatatc
     9361 atggatcaga gacatgcaac ggaggatgac atcaaacgct ggataacgga atttttcagg
     9421 agcaacggat tcgtatgaaa acgatcatcg acgaaacaag agatctggca ttatcgacac
     9481 ataaccatta tctcgaagaa gccagaaaac agggcaaaaa aatcatcgga tatttctgtt
     9541 cctatatgcc tgaggaaatt atccacgcgg ccggctttgt tccctaccgc atgcgggccg
     9601 tggaaagcaa agggacaacc cgtgcggatg ctttttattc ttcgatcaac tgcaccttcg
     9661 tcaagcactg tttcgaaaag gcgctcaaag gagattttgc gtttttggac ggcgttgtgt
     9721 tcgtaaacgg ctgtgatcat tcgcggcgca tgtatgacaa ctggcgtttc gcaaagatcg
     9781 accctccttt tcttcacatg tttttcgcac cgcatatgat caatgaagca gctcttgact
     9841 atttttccct ggagtgcagc aagctaaaat catccatcga gaaacatttt catgtttcca
     9901 taaacgacga atcgctcagg cgctccatca acctttataa tcagaagcgg cgtcttctgg
     9961 cccagatata tgccctgaga aaaatgaagg atgtgcccat aaaagccagt gagttattgg
    10021 gcgttatgct ggccgtcaca gcggttcccg tagaacgggc catagacatc ctgtcgaatg
    10081 tgaaaaatta tatcaccggt cgcgttgtgt ccaaacccca ggatttacgg ctgtttttct
    10141 ccggaggctg cattgaggaa atggatcatc tggaactcat agaaaactgc ggggccgtca
    10201 tcgtggccga taacatctgt ctgggatcgc gtcatttcct tgatgaggtc aaaaatgacg
    10261 gcaatccttt gcaggacatt gccgacagat acttaagcca tttatcctgt ccccggatga
    10321 tgggtgattt tgaacggagg tttaattatt tgttggacgt cagggatgaa tacggcattg
    10381 acggcattat tatcgaaaaa ttaaagtttt gcgatatttg gggcggggaa atatatcttc
    10441 accgcacaga atcaaaaaaa agaggcttgg cgctcttggc cttggagagg gaactctacg
    10501 gcgggggcat cggacaaata aaaacaaggg ttcaggcttt tctggagaag ttgagaaaca
    10561 aacaggcaac cgatgaaaat ctggttcagg cggcaggggc tgattataaa cctaaaatac
    10621 aataaaccgg gagggtaaaa cagatgaaaa aaaaggggag cggttatgaa tatataatcg
    10681 tcggtacggg accggggggc gctccggcag ccagggaact ggccaaagcc ggtaagaaag
    10741 tgctcatggt ggaaaaaggc gattatcaca aaaagtttct ggggcttcct ttcggcatca
    10801 ggctgcttga gcgatacatg ctcttcgccc gctccaggga aggcgtcatt atcgaacgcg
    10861 gcattacggt cggcggttct tccatggttt atcagtccaa cgtcatggat cctcccgtaa
    10921 aactgattaa taatatgggg gtcgatttca gaccggaagc caagcagctt aaaaaagaaa
    10981 tcggtatcga ggttctgccg gaaaaatttt accgcaacaa caagggactc taccggatgc
    11041 tggcgacagc cgataaaatg ggcattccct tccgggagca ggagaagttc gtccatgccg
    11101 ataagtgcaa actgggttgt gactggtgca tgctgggttg tccggaaaac gcgcgctgga
    11161 caaccaggga acacgtggaa gaggctaaat catacggcgc cgagctcatc ttcaatgctc
    11221 cggtcaacaa aatcctcttc aatgccggaa aaacaaaggc aaccggcatt caactgaaga
    11281 acggcaccgt cattcatggc gacaatatca tcctttccgc cggcggtatc ggcagtcccg
    11341 ctgtattgtt aagatcgggg atcaaacgat tagggacgca ggatgtggga acaagattct
    11401 ttatggatcc catgaacatc atgctgggtt acagcaagga tcccgacggc ggcttgtggg
    11461 gaatacagac gttcagtcat gccattgaat ccatggctca gagcgaagga tttatgatcg
    11521 gcaactgttc cgcttttgga acatggacgg ttttgtcttt tttccgcatg cagacagccc
    11581 gagaaaatct gccgaaattt cccatcatga aaagaggcat ggggttattt ctcaaattgt
    11641 ccgatgaagc ccacggacaa attttcccca atgagaaaac cagcaagccc ttcaccgcaa
    11701 atgacgaaaa gagattgaaa aaaggaacgg acattatgcg ggaaatcatc attaagtccg
    11761 gcggcgatcc gaagaccatt tccatcctga aatgggcggg aggacatccc ggaggaaccc
    11821 ttgccatggg acaggc
//
LOCUS       DAOOLB010000067        11578 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_6236_length_11578_cov_39.389482, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000067 DAOOLB010000000
VERSION     DAOOLB010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11578
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6236_length_11578_cov_39.389482"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /gene="guaA"
                     /locus_tag="PLF77_08345"
     CDS             <1..1587
                     /gene="guaA"
                     /locus_tag="PLF77_08345"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF000848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPC08900.1"
                     /translation="KIVVCVVYENQGTGEGEDNVILIIDFGSQYNQLIARRVRENHVY
                     CQIEPPGISIAAIREMEPEGIILSGGPASIYSKGAPRVDKKIFKLGIPVLGICYGLQY
                     MIDSLGGKVISSPKREYGFAELLVKTQKGIFRGVARKTPCWMSHGDSIGELPKGFAVT
                     ATTANTAVAAAQDVRRHFYGLQFHPEVVHTRDGRIMLANFLFGICRCKKTWSMQSFVQ
                     EAVNDIRRQVGREKVLLGLSGGVDSSVAAVLLHKAIGKQLTCVFVDNGVLREGEAQRV
                     IGMFKKHLQINLRFARAGKIFLKKLKGVTDPERKRKIIGRTFIEVFNREARKIKNVKF
                     LAQGTLYPDLIESRSAFGGPSAVIKSHHNVGGLPKTMSLQLIEPLKHLFKDEVRLLGK
                     ELGLADDVVWRQPFPGPGLAIRIIGEVTPQRLSILRKADTILWEEIRARKLYYKLWQS
                     FAVLLPIKSVGIMGDQRTYENILSIRAVTSDDAMTADWAPLPHDVLARISNRIINEVR
                     GVNRVVYDISSKPPSTIEWE"
     assembly_gap    1794..1893
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1894..3095)
                     /locus_tag="PLF77_08350"
     CDS             complement(<1894..3095)
                     /locus_tag="PLF77_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08901.1"
                     /translation="MTSFAYGNVVQESDAQVWKVGRHRWTIHDEYRYGIWIEESVTED
                     FFIRHNIRVDCADVPYAMRWIYARIHHLPAAASTVDNRFIGHWSKDWSHLPTNPHWHK
                     DRRFRAALMSVLSSTSTQTLPSDTYPIKIGRHSVTPGTVFLFARDHAGIVSHVVMDGS
                     TTHPVQTFEASSPARLQGLRLKDFLLPNPNADYISGLLKFRWPVSDGNTWRYLPLEEQ
                     PFYSDEQYLPAFTKGYSNYLEAVEKRINPAVYEPGEKADKIMMTLYRRLNERVPIVLK
                     GYIKCHGIECPEGSLLWEIYSTYNRDDFIGFLLHYLEQIIAKNHLDRNDVLNNMEGIR
                     LQITPGRFVTLRHVLENFKWISSDPEAGIEARWGLDKCSTITTRLRTAQESIVFIQNK
                     YGETDPLFA"
     gene            complement(3338..3544)
                     /locus_tag="PLF77_08355"
     CDS             complement(3338..3544)
                     /locus_tag="PLF77_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08902.1"
                     /translation="MTYKELLEKQIGRECTFTASHTKCVINFGAAEPFASEGIIENVT
                     DDYVIIKSQSSTMIYLLVNTSISY"
     gene            complement(3737..4792)
                     /locus_tag="PLF77_08360"
     CDS             complement(3737..4792)
                     /locus_tag="PLF77_08360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPC08903.1"
                     /translation="MRIKRFFIAFSSYVLICSLVILGYILGFSPVPLRVLVVSQLGIL
                     ACNIVLYIMFRTGLNKRFKDPSLTLLQIVIATCWLLEVLYYADSVRSAVLMDFLIIFI
                     LGLFKFNVREFLFLSFFTVAGYTGVILLLYINRPESLNYKVEILNIAILATVLPWFSL
                     VGGYITRLRKKVALSLATVKESEKKYIELSIIDDLTQLFNSRHFYAQLEKEIERANRY
                     NQPLTLLMLDLDKFKNFNDTYGHLEGDQVLSRIGHVIKRCLRDSDSAYRYGGEEFVIL
                     LPMTKAEDAIITAKRIQETLRKTSFSPVSDQKIFMTVSVGLSQYIPREDLKAFVRRVD
                     TIMYQAKHQGRDRICTG"
     assembly_gap    5035..5134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5135..5345
                     /locus_tag="PLF77_08365"
     CDS             <5135..5345
                     /locus_tag="PLF77_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08904.1"
                     /translation="ENFFDAHLYYQVLNMSMMISLEFSNAQQQASFVIRLLITGNAMG
                     PLQSRLAEGGRGNPGLLNDLLNNFF"
     gene            5399..8065
                     /locus_tag="PLF77_08370"
     CDS             5399..8065
                     /locus_tag="PLF77_08370"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HPC08905.1"
                     /translation="MINIFPENDYNQTLRIKRFLIAFGAYVMWNAITIASYLLGLTKV
                     PLSILVAGVTANIVVNILLYAVFRTGFNKKAKDPSLTLLQMVIATFWIMVVMYYAYEA
                     RGACLLVYMVVLVFGFFRLRVRQFLLLSLYALVNYSAVIFLVYKLHPELINPKLDLLN
                     IIVLAIILPWFAMIGGYINGLRTSITNALTTIERLTDNIKDVIFVVDMNLIYTYVSPS
                     VKTLRGYEPEDVMKQNLADSFAPTSLDTAMETLSEMMEMGKSKSQDSPERTLLLEINR
                     KDGNPVWTETKFSLVRDKKQYITGVLGVSRDISERKRAEEQYRLLADHMTDQVWLFDL
                     NLKFQYFSPSSEKASGYTFTEIKEISLDKLLTEESFQKALEMFSEEMAKAEIVPPPPD
                     YKRSLEVEFRCKDGRLISLDTTLSFIQDKNGKPLSILGESRDVTERKRAEAQIQYLAT
                     HDNLTQLPNRMMFNHLLNQAIKSAKRNKRQLALLFIDLDRFKIINDTMGHDAGDRLLK
                     EMAKRFRQTLRAADVIGRPKDKEDVVGRLGGDEFIILIEDVTDLANVAIVAQKILGTV
                     MKPMVLLGEECRVTSSIGISVYPTDGEDEQTLMKKADMAMYFAKEEGKNNYQFYSQNI
                     QSQSYERFSIETNLRRALERHELYLDYQAKMDFKSGTITGVEALLRWNSPQLGSITPT
                     QFIPVAEETGIIVPIGRWVLKTACVQNVAWQRQGLPPIRVAVNLSLRQLMDDKALEDI
                     KNALDESGMSPNLLELEITESMIMFKPERLIGVLTKIKEMGVRLALDDFGTGYSSLSQ
                     IKHLPIDTLKVDRSFIRNLPHDSDDKAIIEAIIAMGKTLSLNIVAEGVETLEQDHFLR
                     EKICDEMQGFYFSKPVAPDQFADLLRQYNFPS"
     assembly_gap    8069..8168
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8367..10184)
                     /gene="recD"
                     /locus_tag="PLF77_08375"
     CDS             complement(8367..10184)
                     /gene="recD"
                     /locus_tag="PLF77_08375"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HPC08906.1"
                     /translation="MRKHENIPAADYASPLSRGLADFLKRLSLTLGATEEAARTAGDA
                     ARACAQALAEGDVCANFSLLAAAEGKPAQDVRKLLDASLVASSAGADKKLPLVYDDEG
                     QIYLYRYFAYERKLADAIIRRLPGPAARTVSAESRQFLEKRFAENARELSGRPDWQKI
                     AVEMAFTNRLTIISGGPGTGKTTIVTALIAALAINDHPPRIALVAPTGKAAARMEEAM
                     RSQMSTLDAELRKRLPERASTIHMLLGASPAGNTFRFNENNPLPYDLIVVDEASMIDL
                     SLASRLFSALHPDTGIVLLGDKDQLAAVEAGAVFAELAQQTSVPDNMDKLSTGSGTPP
                     LNAQPNEKGPLSSCVVWLKENYRFSAASPIAKVAGLVVNGEGEQLIEWLQNEKGDNIK
                     WEAFDEGLPSRVVGALVKGFEPFVEAVNQGDPEKVLAAYEKFCVLCVIRNGRRGVNGI
                     NDVIARQLRLKISGEGPGEGPWYDGRPIMITENDYGLGVFNGDIGVALKTKDGHRHVW
                     FMAKDGGVRFVPPSSLPAHQTAFAMTVHKAQGSEFENVSLILPEQDTPVLTRELIYTA
                     VTRAKKTLLIYGSADILKLAVGRPTSRRSGLAKKISTPP"
     gene            complement(10190..>11578)
                     /locus_tag="PLF77_08380"
     CDS             complement(10190..>11578)
                     /locus_tag="PLF77_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPC08907.1"
                     /translation="AKGLEWPIVFCPFVWSDRSRSLKDEVCTYHEGDTLVLDYATDDE
                     KKKKQEDEAGEERVRLIYVALTRAANRCYIVAGPFWQPSGKGATEKKAKKSILNWIVA
                     GDEIKHGVWAEKKEVTVDSMHQAWQRLLTSNKGIGGIPWPDKKETVRPVICKEKRKYE
                     ARKVGRLPGVAWKISSYSSLTASSLEGGRQHEPATQDYDVIPLAIEDHDPPQVLRAKL
                     SGDIIDFPAGTTTGHCLHAVFERIDFQDESGWKDIITNVLANYPPVDALEKRDDQVSR
                     IHGMLKDVMSTPLPTGFKLGDVARAKRFSEFEFAYSVRHLDHAKLKNLLDKHGCRIPP
                     LDFPDLEGYLRGFIDLVFEHAGKWYVLDWKSNKLGISAAEYSRSSVDEAMFNHAYEFQ
                     GLIYLIALHRYLKLRISKYDYSRHMGGILYLFVRGVRPAWPSAGIWHKLPEPALIESL
                     DALFSSENRRTV"
BASE COUNT         2779 a         2879 c         2805 g         2815 t
ORIGIN      
        1 aaaatagtcg tatgcgtagt atatgaaaac caaggaacag gagaaggaga agacaacgtg
       61 attctgatca tcgatttcgg atctcaatac aaccagctta ttgcccgccg ggtcagggaa
      121 aatcatgtgt actgccagat tgaaccgccc ggaatttcca tagccgccat ccgggagatg
      181 gaaccggagg gaataatttt atcgggcgga ccggcaagca tctacagcaa aggcgcgccg
      241 cgggtggata aaaaaatctt caaactgggt attcccgtgc tgggcatctg ttacggtctg
      301 caatacatga tcgactctct gggggggaag gtgatcagct ctccgaaacg cgaatacgga
      361 tttgccgagc ttctggtcaa aacacaaaaa ggtattttca ggggcgttgc ccggaaaact
      421 ccctgctgga tgagtcacgg cgattccatc ggagagctgc ccaaaggatt tgccgtgacg
      481 gcaaccacgg ccaatacggc ggtggcggcg gcgcaagatg ttcggcggca tttttacggc
      541 ctgcagtttc atccggaggt tgttcatacc cgggatggca gaatcatgct ggccaatttt
      601 cttttcggaa tctgccggtg caaaaaaacc tggtccatgc agtcctttgt ccaggaagcc
      661 gtaaacgaca tccggcggca ggtaggccgt gaaaaagtcc ttctcggact ttccggcggc
      721 gtggattcgt ccgttgctgc cgtgctgttg cataaggcga tcggcaaaca actgacctgt
      781 gtttttgtgg acaacggtgt cttgcgagaa ggggaagcgc agcgcgtgat cgggatgttc
      841 aaaaaacatt tgcagatcaa tctgcgcttt gcacgggccg gcaaaatatt tctgaaaaaa
      901 ctgaagggcg tgacggaccc ggaaaggaaa cgcaaaatca tcggccgtac cttcatcgag
      961 gtatttaatc gcgaagcacg caaaatcaaa aatgtgaaat ttctggctca aggaaccctg
     1021 tatccggatt taatcgaatc gcgttcggcg ttcggcggtc ccagcgccgt gatcaaatcg
     1081 catcataatg tcggcggcct tcccaaaacc atgagcctgc aactgataga accgctgaag
     1141 catttattca aggatgaagt aaggcttttg ggaaaagaac tgggtctggc ggacgatgtc
     1201 gtctggcggc agccgtttcc cgggccgggt ctggccatcc ggattatcgg tgaggtgacg
     1261 ccgcagcgcc tgtcgatttt gcgaaaagcc gatacgattc tctgggaaga aatccgcgcc
     1321 agaaagttgt attacaaact gtggcagtcc tttgccgttc ttctgccgat caagagcgtc
     1381 ggcattatgg gcgatcagcg cacctatgaa aacattttgt cgattcgcgc ggtcaccagc
     1441 gacgacgcca tgacggccga ctgggcgccg ctgccgcacg atgttctggc ccggatatcc
     1501 aaccggatta tcaacgaagt ccgcggcgtc aatcgtgttg tttacgacat cagttccaaa
     1561 ccgcccagca ccatcgaatg ggaataggtc gcggtaatgc cgggacgctc gcagtgaaat
     1621 agatttttgg agcgggcgag acaatcatta tcgaagtgcg tttttcatgc agttgttttc
     1681 caggctttca ctggtcattt cgtcaacgat tttctgttgt ttcaggatgg actgttcggc
     1741 aaacagagga tccgtctcgc cgtatttgtt ctgaataaac acgatagact cctnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcaaaca gaggatccgt ctcgccgtat
     1921 ttgttctgaa taaacacgat agactcctgt gcggtccgga gacgggtcgt aatggtactg
     1981 catttatcca gtccccagcg ggcttcaata ccggcctcgg gatcggaaga aatccatttg
     2041 aagttttcca gcacgtgtct tagagtcaca aaccggcccg gagtaatttg caggcgaatg
     2101 ccctccatgt tattaagcac gtcatttctg tccaggtgat ttttggcaat aatctgttca
     2161 aggtagtgca acagaaaacc gataaagtcg tcccgattgt acgtgctgta aatttcccac
     2221 aaaagagagc cttccgggca ttctatgcca tgacatttta tataaccttt aagaacaatc
     2281 gggacgcgtt cattcaaccg gcgatagagc gtcatcatta ttttatccgc cttttcgccg
     2341 ggctcataga ctgccgggtt gattcgcttt tccaccgctt cgagataatt ggaatatcct
     2401 ttcgtgaaag ccggcaggta ctgttcatcg gaatagaacg gctgctcctc cagcggaagg
     2461 taccgccacg tgttgccgtc cgagaccggc cagcggaact tcagtaagcc ggaaatataa
     2521 tcggcattcg gattgggcaa gagaaaatcc ttgagcctta atccctgaag ccttgccggt
     2581 gaactggctt caaaggtttg gaccggatgg gtggtgctcc cgtccatgac gacatggctc
     2641 acaataccgg catgatctct ggcaaaaagg aaaacggttc ccggtgttac ggagtgccgg
     2701 ccgattttta tcggatacgt atcggacggc agggtctgtg tggaggttga tgacaatacg
     2761 ctcattaatg ccgcccggaa acgtctgtct ttatgccagt gcggatttgt cggtagatgg
     2821 ctccagtctt tggaccagtg tccgatgaac cggttatcaa ctgtacttgc ggctgccggg
     2881 agatgatgga tgcgtgcata gatccaccgc atggcatagg gaacgtcggc acagtccacg
     2941 cgaatattat ggcgaatgaa aaaatcctcc gtaacgcttt cttctatcca gatgccgtat
     3001 cggtattcgt catgaatcgt ccaccggtgc cggccgactt tccatacctg agcatccgat
     3061 tcctgaacaa cattaccgta tgcaaatgat gtcataagcg tacaggccgc gaagcctatc
     3121 agtatgcagc gaaggaaaaa cattataacc ccgttttgca aaaatccgaa aatcgtcaaa
     3181 accataccat caattatttt ttatacaaga aaaacatcaa ccgcgttttt gccctgcgac
     3241 ggtgaaagtc atattcggga aacatggcaa aaaccggtaa aaatagaacg gggaaaatta
     3301 ccgccggtat ctgatttttg tccgtttgtg gcttttgtta atacgatatg gaggtattga
     3361 ccagaagata aatcatggtt gaactttgac ttttgataat gacatagtca tcggtaacat
     3421 tctcgatgat tccctcgctg gcaaagggtt ctgccgctcc gaaattaatt acgcacttgg
     3481 tatggcttgc ggtaaacgtg cattcccttc caatctgctt ctccaagagt tctttgtaag
     3541 tcattgtgcg attcctttct tcaaaaagtt gttttcgtca cacgttgatg gcttttcgaa
     3601 agtcctcttc atcaagagca ttacaccgtc aaccattatt cgtaattata atagcacgat
     3661 tccaattttt ttaaagaata aaatagggat attttttctc ttgatgcgtg ccttttaagg
     3721 ggcaacccct gattcgctag ccggtgcaaa ttctgtctct gccctgatgt ttggcctggt
     3781 acatgatcgt gtccacccgg cgaacaaagg ctttcagatc ctctctcggg atgtactgcg
     3841 agagaccgac gcttactgtc ataaaaattt tctgatccga cacgggggaa aacgaagttt
     3901 ttctaagcgt ttcctggatc ctttttgccg ttattattgc atcctccgct tttgtcatcg
     3961 gcagtagaat tacgaattct tcgccgccgt agcgataggc ggaatcggaa tcgcgcaggc
     4021 atcgtttgat gacatgaccg atcctggata acacctgatc gccctcaagg tgcccatagg
     4081 tgtcgttgaa attcttgaat ttatcgagat ccagcatcag cagggtcaac ggctggttgt
     4141 aacgattggc tctttcaatt tccttttcca gttgagcgta aaaatgtctg gaattgaaaa
     4201 gttgcgtcag atcgtcaatg atgctcagct cgatatattt tttttcgctt tctttgacgg
     4261 tggcaagaga gagggccacc ttttttctga gccgggtgat gtatccgccg accagtgaaa
     4321 accagggcaa aacagttgcc aggatggcaa tattcaatat ttctacctta taattaaggg
     4381 attcgggacg attgatataa agcagcaaaa taacgccggt gtagccggca acggtgaaaa
     4441 acgagagaaa aagaaattcc ctgacattaa acttgaaaag gcccagaata aaaataatca
     4501 gaaagtccat caacaccgcg cttctgacgg aatcggcgta atataaaacc tccaaaagcc
     4561 agcacgtggc aattacgatc tggagaagcg tcaggctggg atccttgaat cttttgttca
     4621 atccggtcct gaacattata tataatacaa tattacaggc cagaatcccc agctgggaaa
     4681 caaccagcac gcgcaaagga acgggggaaa atccaagaat ataaccgagg atcaccagag
     4741 aacaaatcag gacgtacgat gaaaaggcga tgaaaaatct tttgattctc aacgcctgcc
     4801 tgcgatcgtc tttgggaatt atactgaaaa tatattttct cctttataag ccggttcatt
     4861 gtgtttgccg actgcccgga ttttttcaaa aagagagtgt cttactgcaa tccgtttcaa
     4921 cgcccccagg ttgttcgcaa gtcgcttgcc ggaccggcac gaaccgatgt tcacaccctt
     4981 gaaaattttt ttgatgcgca tttatattac caagttctga atatgtcaat gatgnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgaaaa tttttttgat gcgcatttat
     5161 attaccaagt tctgaatatg tcaatgatga tcagcctgga atttagcaat gctcaacaac
     5221 aggcgtcgtt tgtgatccgt ctgttgatca ccggaaatgc gatgggcccc ctgcaatccc
     5281 gattggccga gggagggcga ggcaatccgg gtctattaaa tgatttactt aacaactttt
     5341 tttgagtata tttgtttcaa tccagaaagg gagatgttgc ggtaataaaa aaatattcat
     5401 gatcaatatc tttcctgaaa atgactacaa tcagacttta agaatcaagc gcttcctcat
     5461 cgcttttggc gcgtatgtga tgtggaacgc cattaccatt gccagttatt tactgggact
     5521 gacaaaagtg ccgttatcca tcctggttgc cggagtcacc gcaaatattg ttgtcaatat
     5581 tcttctgtat gccgtgttca gaaccggatt caataaaaaa gccaaagatc cgagtctgac
     5641 tcttcttcaa atggtgattg ccaccttctg gattatggtg gtcatgtact atgcctacga
     5701 ggccagaggc gcgtgccttc tcgtttacat ggttgtgctc gtgttcggat ttttccggtt
     5761 gcgcgtccgg cagttcctgc tgttgtcgtt atacgcgttg gttaactatt cggctgttat
     5821 ttttcttgtt tacaagctgc acccggaatt gatcaatccc aaactggatt tattgaacat
     5881 tatcgtcctg gccattattc ttccgtggtt tgccatgatc ggcggataca tcaacggact
     5941 ccggaccagc ataaccaatg cccttacgac catcgagcgg ctgacggaca acatcaaaga
     6001 tgttattttc gtggtggata tgaatctgat ctacacctat gtcagcccgt cggtcaaaac
     6061 cctgagaggc tacgaaccgg aagacgtaat gaaacagaat cttgccgatt cctttgcgcc
     6121 cacctccctg gacaccgcga tggaaacgtt gtccgaaatg atggagatgg gcaaatccaa
     6181 aagccaggat tcccctgaga gaacccttct cctggaaatc aaccggaaag acggcaatcc
     6241 cgtatggaca gaaaccaagt tttccctggt cagagacaag aaacaataca tcaccggggt
     6301 tctgggcgtc agccgggata tttccgagcg caagcgtgcc gaggaacaat accgtcttct
     6361 ggcggatcat atgaccgacc aggtgtggct cttcgatctg aacttgaagt ttcaatattt
     6421 cagcccgtct tcggagaagg catcaggata cacgtttacc gagattaaag agatctccct
     6481 ggataaactt ctcaccgaag aatctttcca aaaagcgctg gaaatgtttt cagaggaaat
     6541 ggccaaggcg gagattgtcc cccccccgcc cgactataag cgttccttgg aagttgaatt
     6601 tcgctgcaag gacggtcgtc tcatatcgct cgataccacg ttaagtttca ttcaggataa
     6661 aaacggaaaa cctttatcca tcctggggga aagccgcgat gtgaccgaac gtaagcgcgc
     6721 cgaggcccag atccaatacc tggcaacaca cgataatctg acccagttac ccaatcgcat
     6781 gatgttcaac catttgctga atcaggccat taagtccgcc aaacgcaaca agcgtcagct
     6841 ggcgcttctc tttattgatc tcgaccggtt caaaatcatc aatgacacca tggggcatga
     6901 tgccggcgac agactattga aagagatggc caaacgattt cggcaaaccc tgcgcgccgc
     6961 tgacgtgatc ggacgcccca aggataaaga agatgttgtc ggacgtctgg gaggcgatga
     7021 attcatcatc ctcatcgagg atgtaaccga cctggccaac gtcgccatcg tcgcccagaa
     7081 aatcctcggc accgtcatga aaccgatggt tctgctgggt gaagaatgcc gcgtcacatc
     7141 gagcatcggc atcagtgtgt accctacgga tggagaggat gaacagaccc tgatgaagaa
     7201 agcggatatg gccatgtact ttgccaagga agaagggaag aacaactacc agttttattc
     7261 ccagaacatt cagtcgcagt cctacgagcg tttttccatc gaaaccaatc tgcgccgggc
     7321 gctggaacgc cacgaactgt atctggatta tcaggccaag atggacttca aatccggcac
     7381 gatcacgggt gtggaagccc tgctgcgctg gaacagcccg cagctggggt ccatcacgcc
     7441 gacccaattt attccggtcg ccgaagaaac cggcatcatc gtgcccatcg gccggtgggt
     7501 cttaaaaacc gcctgcgtcc agaacgtcgc ctggcagcgc cagggcctgc cgcccatccg
     7561 ggtggcggtc aatttatcgc ttcgtcagtt gatggatgat aaggctctag aagatatcaa
     7621 aaatgctttg gacgaatccg gtatgtcgcc gaatctactg gaactggaaa tcacggaaag
     7681 catgatcatg ttcaaacccg aacgcctgat cggtgtgctg accaaaatca aggagatggg
     7741 tgtgcgcctt gcccttgatg atttcggcac cggctattca tccctttccc agatcaagca
     7801 tctccctatc gataccctca aggtggacag atcgttcatc cgtaatcttc ctcatgattc
     7861 cgacgacaaa gcgatcattg aagccatcat tgccatgggc aagacgctga gccttaatat
     7921 tgttgccgaa ggtgtggaga cactggaaca ggatcatttc ctgcgggaga aaatctgcga
     7981 cgaaatgcag ggattttatt tcagcaagcc tgttgcaccg gatcagtttg ccgatctgct
     8041 gcgccagtac aatttccctt cataaaaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8161 nnnnnnnntg caccggatca gtttgccgat ctgctgcgcc agtacaattt cccttcataa
     8221 aaaaatttcc tgaacattag ccgaagaaaa actctcagcc gtctctccaa aaatatttac
     8281 gcgccggggt ttgaaaaaac ggcttgggtt gtgttctaca gagggggggg cgtcgaaaga
     8341 ttgccggatt cggtccgtgt cggcggtcat ggcggcgtac taattttttt cgccaggccg
     8401 ctgcgacgcg atgttggacg gcctacggcc agcttcagaa tgtccgcgct gccgtatata
     8461 agaagagtct tcttcgcgcg ggtaaccgcc gtatagataa gttccctggt caacacgggg
     8521 gtgtcctgct cgggcagaat aagcgagaca ttttcgaatt ccgacccctg ggctttatgc
     8581 accgtcatgg cgaaagccgt ttgatgagcc ggcaaggaag aaggaggtac aaaacgcacc
     8641 cctccgtctt tggccatgaa ccagacgtga cgatggccgt ctttcgtctt gagggccaca
     8701 ccgatgtcgc cgttgaatac cccaaggcca taatcattct ccgtaatcat aatcggacga
     8761 ccgtcatacc aaggcccctc accaggtcct tctcccgata tcttcaggcg taactgccgc
     8821 gctatgacgt cattgatacc gttgacaccc cgtctgccgt tacggatgac gcaaaggacg
     8881 cagaattttt cgtaagcggc aagcaccttc tccggatcgc cctgatttac cgcctcaaca
     8941 aacggttcaa accccttgac aagagcccct acaacacgcg acggcagtcc ttcatcaaag
     9001 gcttcccact tgatgttatc ccctttttca ttttgaagcc attcgatgag ttgttccccc
     9061 tcaccgttaa cgacaaggcc cgccacttta gctatgggcg acgctgcgct gaaccggtaa
     9121 ttctccttga gccagacgac acaactggac aacggtcctt tctcgtttgg ttgagcgttg
     9181 agaggtggtg tgccgcttcc tgtcgaaagc ttgtccatat tgtcaggcac cgatgtctgc
     9241 tgcgccagtt ctgcgaatac cgctccggct tcgactgccg ccaactgatc cttatccccc
     9301 agcaaaacaa ttccggtgtc cggatgcagt gcggaaaaca gccttgaggc cagggataaa
     9361 tcaatcatgg atgcttcatc gacgacaatc aaatcatagg gtaggggatt gttttcgtta
     9421 aagcggaacg tattgccagc cgggctggct cccagcagca tatgaatggt ggaggcccgc
     9481 tcgggcaggc gtttccttaa ctccgcgtca agcgtgctca tctgactgcg catcgcctct
     9541 tccattcttg ccgccgcttt gccggtcggc gcgacaagcg cgattcgcgg gggatgatca
     9601 tttatcgcca gtgccgcgat gagggctgtt acgatggttg ttttgccggt accgggtcct
     9661 ccgctgatga tcgtcaggcg gttcgtgaat gccatttcta ccgcaatttt ctgccagtcc
     9721 ggccggccgg atagttccct tgcattttcc gcaaaccgtt tttcgagaaa ctgtcgcgat
     9781 tcggcgctga ctgtccgcgc cgcgggcccc ggtaaccgcc tgataattgc atcggccagt
     9841 ttccgctcat aggcaaagta ccggtacagg tagatttgtc cttcatcgtc atacacgagg
     9901 ggcaattttt tatccgcccc ggcggatgag gccaccaaag aagcatccag cagctttcgt
     9961 acatcctgtg ccggcttgcc ttccgctgcg gccagcagag agaaattcgc gcagacatcg
    10021 ccttcggcca gggcttgagc gcacgccctg gcggcatcgc ccgctgtgcg cgccgcctct
    10081 tccgtagcac caagggtcaa agaaaggcgc ttcagaaaat ccgcaagtcc cctggacagg
    10141 ggcgatgcat aatcggccgc aggtatgttt tcatgttttc tcatcaacgc tatacggtcc
    10201 tccggttttc tgaagagaac agggcatcca gtgattcgat cagcgccggc tcgggcagtt
    10261 tatgccagat acctgccgaa ggccatgccg gacgaacgcc acggacaaag agatacagaa
    10321 tgcctcccat gtgccggctg taatcatatt tgctgatacg cagtttcaag taacgatgaa
    10381 gagcgataag atagatcagg ccctgaaatt cataggcatg attgaacatt gcctcatcga
    10441 cggacgaccg ggaatactcc gcggcactga tgcccagctt gttcgatttc caatccagta
    10501 cgtaccattt gcccgcgtgt tcaaagacaa gatcaatgaa accccgcagg taaccttcga
    10561 gatcgggaaa atccagcggg ggaatccggc agccgtgttt gtcgagaaga ttcttaagtt
    10621 ttgcgtgatc aaggtgacgt accgaatagg cgaactcgaa ttcggaaaat ctttttgccc
    10681 tggcaacgtc gccgagttta aacccggttg gcagaggggt gctcatgaca tctttcagca
    10741 taccgtgaat ccggctgacc tgatcatcgc gcttttctaa ggcatccacc ggcggataat
    10801 tcgccagcac gttggtgatg atgtctttcc atccgctttc gtcctgaaag tcaatccttt
    10861 cgaaaacggc atgcagacaa tgtcctgttg tggtaccggc cggaaaatcg atgatatcgc
    10921 ccgaaagctt tgcccggagg acttgaggcg gatcatgatc ttcaatcgca agggggatga
    10981 cgtcataatc ctgtgtcgcc ggttcgtgtt gccttcctcc ttcaagggag gaggcggtaa
    11041 gcgatgagta actggatatt ttccaggcca cacccggcag tctgccgacc ttccgggctt
    11101 catattttct cttctctttg caaataaccg gacggactgt ctctttttta tccggccacg
    11161 gaatccctcc gatccctttg tttgatgtca ggagtctctg ccatgcctga tgcatggaat
    11221 caacggtcac ctcttttttt tccgcccaca cgccgtgttt tatctcatcg ccggcgacga
    11281 tccagttcag gatgctcttc ttcgcctttt tttccgttgc accctttccc gacggttgcc
    11341 agaaaggacc agcgacaata tagcagcggt ttgccgccct cgtcagcgca acataaatca
    11401 gtcgcacccg ttcttcgccg gcctcgtctt cctgtttttt ctttttttcg tcgtcggttg
    11461 cataatcaag caccagggta tcgccctcgt gataggtgca gacctcatcc ttcaatgacc
    11521 ggctgcggtc gctccagacg aacgggcaaa agacaatcgg ccattccagg cctttggc
//
LOCUS       DAOOLB010000068        11380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_6376_length_11380_cov_47.586225, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000068 DAOOLB010000000
VERSION     DAOOLB010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11380
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6376_length_11380_cov_47.586225"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="PLF77_08385"
     CDS             <1..217
                     /locus_tag="PLF77_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08908.1"
                     /translation="PLYENKNAKLFIEQFCAAEHTKTVPMFQGIKQAIIRYPDQDTDI
                     DNWDFYRSLKNKSQRSFTDLWLHGNVF"
     gene            complement(225..872)
                     /locus_tag="PLF77_08390"
     CDS             complement(225..872)
                     /locus_tag="PLF77_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPC08909.1"
                     /translation="MKKILSVIVGFMMLMMIPGISIGSDASPAKKAKAAKFKAAAVNK
                     PYVSVQGGVAFLTDSDVDAGIRSTAMDFDPGYALGVAAGYKMGKYRVEGEIGYQKNEV
                     DKALSGRRTSGDMKAYSFLVNGYYDIATKTRFTPYLTAGIGVAKVDGDFNRIAHVDDT
                     AFAYQVGAGVAYAVNRALAVDLKYRYMDTVDDFGFGNTNPEFSTHNVFLGLRYCF"
     gene            complement(1165..2385)
                     /locus_tag="PLF77_08395"
     CDS             complement(1165..2385)
                     /locus_tag="PLF77_08395"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC08910.1"
                     /translation="MKTSGKTALLFTLYLSQGLPFGFQATALPVLLRDKGISLTTIGF
                     AGALALPWFFKPLWAPLIDRFWSARIGRRRSWIIPLQTMMVLTMFSSVFIAASGSIPL
                     LLLCIFLMNLCAATQDIAVDGLAVDILQRDELGPGNAAQVAGYKAGMVLGGGILVWLS
                     ARSGWESLFLGMAALAVLPLIVILCHREVEPDGSIVSKPPKFSDFISAMRNALQSPKA
                     GYFLLFIATYKIGESMIDMMFKPFLVDNGFTVQQIGLWVGTWGMASSLIGSVAGGWLA
                     ARISIVRALFLAASLRLIPLIGELWLSTFVPTAAQVVSVTVAEHFFGGMLTTALFAFM
                     MSRVDKKIGATHYTVLAAIEVFGKFPGTVFSGCLAENIGYTGLFSFGVVLSLLVLLLY
                     FPLRESTSPPKMNI"
     gene            complement(2464..4146)
                     /locus_tag="PLF77_08400"
     CDS             complement(2464..4146)
                     /locus_tag="PLF77_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPC08911.1"
                     /translation="MTDETASTPTDFIRDIINDDLKTNKNNGKVATRFPPEPNGYIHI
                     GHAKSVCLNFGIARDYKGTCNLRLDDTDPAGESMEYVQSIINDIRWLGFDWEDRLFYA
                     SDYFEKLYQFAVELIKSGNAYVCSLNPDEVREQRGTFTEPGKESPYRNRSVEENLDLF
                     QRMRAGEFADGAHTLRAKIDMASPNIVMRDPVIYRIKREPHYRTGTQWVIYPMYDFAH
                     CLSDALEGIVYSICTLEFENNRPLYDWIVEKLITGLRPRQIEFARLNLSYTVLSKRRL
                     IELVEKKYVRGWDDPRMPTVSGMRRRGYTAEAIRNFCAGIGVAKNDNLIDVALLEHCV
                     REDLNEHAPRAMCILRPLRVVIDNYPDNQVEQIACANHPQKPDMGTRNVPFSKVLYIE
                     RDDFMEDPPKKYHRLGPGREVRLRNAYVIKFESMMKDEKTGDVTELHCSYDPATLNGP
                     PADGRKVPGVIHWVSAGHAVPAEVRLYDRLFSIENPGSEEDFLKYLNPDSLETLTTCR
                     AEQNLKNAAAGSRFQFERLGYFCVDAKDSTSERLVFNRVVPLRDSWAKIAGK"
     gene            complement(4296..4904)
                     /locus_tag="PLF77_08405"
     CDS             complement(4296..4904)
                     /locus_tag="PLF77_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08912.1"
                     /translation="MKGYFKIISMFVGMCVLLCASWADAGVYYTTVQQPPAASEKIKA
                     GTDFSVLPIDYSAINPKDLGYTNNAEWDADKSAVPKAFADAFPVLLKEANITNKKISV
                     VSGGDKITQGVIAQVAVTKINLNWNAWTNRPDEYVCKITFTDAADGQKLFSGEVNVST
                     FSGNPFTSAWGSFSGRMQSAAYNMAWVLTKIMTQGRIEPAEH"
     gene            5144..5965
                     /locus_tag="PLF77_08410"
     CDS             5144..5965
                     /locus_tag="PLF77_08410"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPC08913.1"
                     /translation="MNEKTGNASSPGFYGRPTRQDGQLSAVPAAHLRLSAHFRMVIFI
                     ATLCLSFCALNCRAGVEQNDDVLRKKMVEEQLKARGIKNPQVLRAMSKVERHQFVPEA
                     LRKMAYADSALPIGEGQTISQPYIVAWMTEVINPQADMKVLEIGTGSGYQAAILAELC
                     HSVYTIEIIETLAKRAEKILGKHYQNVHVRTGDGYQGWPEAAPFDAILVTCAPTKVPA
                     PLAKQLKEGGRMVIPVGNVKAQELYVLVKRNGRLEQQAIIPVLFVPMLDKEGEKY"
     gene            complement(6066..6521)
                     /locus_tag="PLF77_08415"
     CDS             complement(6066..6521)
                     /locus_tag="PLF77_08415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HPC08914.1"
                     /translation="MTKQETINSAVSFFNEGYACSQSVLLAFAGRFGLDEKQAKRIAS
                     TFGGGMGRLREKCGAVTGGFMVLGLAYGNADPNDMETKLTAYRKVRGLNRKIEDLHGS
                     SCCSELLKKHATTADVTERKHHQIICQKIVGGVAGILYDMLEEEPPRAR"
     gene            6813..7463
                     /locus_tag="PLF77_08420"
     CDS             6813..7463
                     /locus_tag="PLF77_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HPC08915.1"
                     /translation="MNVKTQELFNFYSHLAGMIAAVIGTVYLAIEASFSASGLVTALI
                     YGISVVFLFSASSLYHAFKKKENELSFWRRMDRLAIFFMIAGTFTPVCYFCLEGNQRW
                     AMIAFQWSLVGVGVISQIFFPRAPRTLYAMLYLFMGWSGLFIIKQVLASMSISQTVLL
                     FTGAGAFTAGGIIYAIKKPKLIPGIFSFHELFHILVLIGGALHYAMIYGVYAQLGA"
     gene            7475..8389
                     /locus_tag="PLF77_08425"
     CDS             7475..8389
                     /locus_tag="PLF77_08425"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPC08916.1"
                     /translation="MQCTPPDTAAACLLAAEADLFLKEYQQHYSAGETKPQQIGMPFF
                     LHAPGSDTGVLLIHGLMAAPEEVREWAEFLHQKGLTVYAPRLAGHGTSCKDLSTRRYE
                     DWLDSAERGHAILKKYCDKILVAGFSTGAGLALQSVILKPHDFEAVIAVSAPLKFKSF
                     SSRFAEVLNAFNRFCHSRGVNTLARDYIKNDADNPHINYLLCPVSAFVQVKKLMKKVN
                     RSLPDIRIPALVIQAKNDPKVAPQSGPEIFRRLGTNKKRFAWIDHDLHGIVRGNISGM
                     VFKEVELFLAEQNLIHIRPEMSNRLPGA"
     gene            8673..>8862
                     /locus_tag="PLF77_08430"
     CDS             8673..>8862
                     /locus_tag="PLF77_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPC08917.1"
                     /translation="MSEGKVKWFNESKGFGFIEKEEGGDVFVHFSSIQGEGFKTLNEG
                     QRVSFDVAQGKKGQEATNV"
     assembly_gap    8863..8962
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8963..10271
                     /locus_tag="PLF77_08435"
     CDS             <8963..10271
                     /locus_tag="PLF77_08435"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08918.1"
                     /translation="SSESGVDLNRSRLSQIKGISFLDDGGGYVFTGTRPPISDLDGLA
                     DCDLTLIKYLQKRWSYIPINRGRGCNWNCEFCVVNKQYGKYKAASIDTALRQLIRYAD
                     MGYTKFFFTDDNFAQHPDEAIALCKAIGNYKRNFLKKIDLVVQVRSEVAENDALIEAM
                     HFAGVSMLAIGYESPINEELRAMRKGVTVEKLIARSRKLSNVFYLHGMFIFGYPLNDD
                     AQGPSSFTVKQRAKIYKDFFKKSGIDTIQVLNAVPLPGSDLRTRLEREGRLLPLKIVG
                     WDKYDGLFLCYDPRPDSMDAFELQGMPGLLMKKKYLGGFIKRNLNYGNWLNWTYIATI
                     GFPIQFVEFYIRRFIRNLAERQRAGKRPDGAWSPRTIFAVPLISAWKDIKRIWRNLFV
                     RTYAGAIVNRWLKIYRKSRYGDKLRKFFLDSKITPKSRHRLNT"
     gene            10494..11380
                     /locus_tag="PLF77_08440"
                     /pseudo
     CDS             10494..11380
                     /locus_tag="PLF77_08440"
                     /inference="COORDINATES: protein motif:HMM:NF004042.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
BASE COUNT         2880 a         2867 c         2725 g         2808 t
ORIGIN      
        1 ccctttatat gaaaataaaa acgcaaaatt gttcatagaa caattttgtg ccgcggagca
       61 taccaaaact gtaccgatgt ttcaaggcat taaacaggca atcatccgtt atccggatca
      121 agacacagat attgataatt gggatttcta cagatcacta aaaaacaaaa gccagaggtc
      181 ttttacagac ctctggcttc atggaaacgt tttctaaaaa tctattagaa gcagtatctc
      241 aagcccagga atacattatg tgttgagaat tcgggattgg tgtttccgaa accaaaatca
      301 tcaaccgtat ccatgtaacg atacttcaaa tcaacagcaa gagctctatt gacggcataa
      361 gcaacaccgg cgccgacctg atatgcgaag gcggtgtcat cgacatgagc aatgcggtta
      421 aaatcgccgt cgacttttgc aacaccgata cctgcggtca ggtaaggtgt gaatctggtc
      481 ttggtggcaa tgtcataata gccgtttacc aggaaggaat aagccttcat atcgcccgag
      541 gttctccggc cggaaagagc tttgtcaact tcattcttct gatagccgat ctcgccttcc
      601 actctgtatt tgcccatctt gtaaccggct gccacgccca gtgcataacc cggatcaaaa
      661 tccatggcgg tgcttctgat gcctgcgtcc acatcgctgt ccgttaaaaa ggccacgccg
      721 ccctgtacgc ttacatacgg tttgtttact gccgctgctt taaattttgc tgctttggct
      781 tttttggcgg gtgatgcatc gctgccaatt gaaatcccgg gtatcatcat caacatcata
      841 aatccgacaa tcactgataa aatctttttc ataaatcgtc tcctttttaa attttttttt
      901 gacttaaccg tgtttcatac ttcttggaaa ctacaacaaa acaacctctt caatgtgtat
      961 ataaataaaa aatttggctc tacatgtcaa gcaatttcta acccggatgt tcgcgtcacc
     1021 aaaacgtttt ttcctgaacc ggcgtaaacg gccttgatca ttgattattt tttttcaatt
     1081 tataaactat ccgccccaag gcggtaaaaa tataatttca aaaacatttt cgggatcatg
     1141 actatttttt tcatcaattt ccttttatat attcattttc ggcggcgacg ttgattcccg
     1201 cagcgggaaa tagagcagca gcaccagaag cgacagcacc actccgaaag aaaaaagtcc
     1261 cgtataaccg atattttccg caagacagcc cgagaaaaca gtccccggaa attttccaaa
     1321 aacttcaatg gccgccagca cggtataatg cgtggcgccg attttcttgt cgacacgcga
     1381 catcatgaac gcgaaaagcg ccgtggtcag catcccgcca aaaaaatgct ccgcaacggt
     1441 aacgctcacc acctgcgccg cggtgggcac aaacgtggac agccagagct caccgattag
     1501 agggatcaac ctcaatgacg ccgcgaggaa aagggcgcgt acaatggaaa tcctggctgc
     1561 aagccatcca ccggcaaccg acccgatcag cgatgacgcc attccccagg tgccgaccca
     1621 caatccgatt tgctggacgg taaaaccgtt gtctacgaga aaaggtttga acatcatgtc
     1681 gatcatcgat tcaccgatct tgtacgtggc gatgaacaaa agaaaataac cggccttggg
     1741 agactgcagg gcgttgcgca tcgcgctgat aaaatcggaa aatttcggcg gcttgctgac
     1801 aatgcttcca tccggttcca cctcccgatg gcacaaaatg acaatcagcg gcaggaccgc
     1861 cagcgccgcc atgcccagaa acaggctctc ccatccggaa cgggcgctca accagaccag
     1921 tatgccgccg cccagaacca tgcccgcctt gtaaccggcg acctgtgcgg cattgccggg
     1981 accgagttca tcacgttgca aaatgtccac cgccaagccg tcaacggcaa tatcctgtgt
     2041 ggcggcgcaa agattcatga gaaaaataca caacagcagc agaggaattg aaccggatgc
     2101 ggcaatgaag acggacgaaa acatcgtcaa aaccatcatg gtctgcaggg gaataatcca
     2161 cgaacggcgc cgtcctattc ttgccgacca gaagcggtcg atcaacggcg cccagagggg
     2221 tttgaaaaac cagggcaggg ccagcgctcc ggcaaaaccg atcgtcgtga gcgaaatgcc
     2281 tttgtcgcgg agaagaaccg gcaaagccgt ggcctgaaag ccgaaaggaa gaccctgtga
     2341 aaggtagagc gtaaacagaa gtgccgtctt gccgcttgtc ttcatggtgc ggatctctcg
     2401 attcagaaat tacatcgcta tctacttcat gcggcaaaac gggctgtttt caaaacgttt
     2461 tcattatttg ccggcaatct tcgcccagga atctctcagc gggacaaccc ggttgaacac
     2521 gagcctttcg gatgtcgaat cctttgcatc cacgcaaaaa tatcccagtc tttcaaactg
     2581 aaaccggctg cccgctgcgg catttttcag gttttgctct gcccggcagg ttgtcagcgt
     2641 ctccagcgaa tccggattca gatatttcag aaaatcctct tcgctcccgg gattttcaat
     2701 actgaacaac ctgtcgtaaa gccggacttc ggcgggaaca gcgtgtccgg ccgataccca
     2761 gtgaatgacg cccggaacct tgcgtccatc ggccggcggg ccgttcagtg tcgccggatc
     2821 atagctgcag tgcagctcgg tgacgtcgcc ggttttttcg tctttcatca tgctttcaaa
     2881 ttttataacg taggcgtttc tcaaacgcac ttcgcgtccg ggaccgagac ggtgatattt
     2941 tttcggcgga tcttccatga agtcgtcgcg ctcaatgtag aggactttgg aaaagggaac
     3001 gtttcgtgtg cccatgtccg gtttttgcgg atgattggcg caggcgatct gttccacttg
     3061 attgtccgga taattgtcga tgacaacgcg cagcgggcgc agaatacaca tcgcccgggg
     3121 agcgtgctcg ttcaggtcct ctcgcacaca gtgttccaga agcgccacat cgatcagatt
     3181 atcgtttttg gccacgccga tcccggcgca aaaattgcgg atggcttccg ccgtataacc
     3241 gcggcggcgc attcccgaca cggtgggcat gcgcggatcg tcccaccccc gaacatactt
     3301 tttctccacc agttcgatca gccttcgttt actcagtacc gtataactca aattgaggcg
     3361 ggcaaattcg atctgacgcg gacgcagacc ggtaatgagt ttctccacaa tccagtcata
     3421 gagcggacgg ttgttttcaa attccagggt gcagatggag taaacaatcc cttccagcgc
     3481 atcggaaagg caatgcgcaa agtcgtacat cggataaatg acccattgcg tgccggtgcg
     3541 gtaatgcggt tcgcgcttga tgcggtagat cacgggatcg cgcatcacga tattgggcga
     3601 ggccatatcg attttggcgc gcagggtatg ggcgccgtcg gcgaattcgc cggcgcgcat
     3661 ccgttgaaac aaatcgagat tttcttcaac cgagcggttt cggtaagggc tttccttgcc
     3721 cggttccgtg aacgttccgc gttgttctct gacttcatca ggattaagag aacagacata
     3781 ggcattgccg gatttgatga gttcaaccgc aaactgataa agtttttcga aataatcgga
     3841 ggcgtaaaac agccggtctt cccagtcgaa ccccagccat cggatatcgt tgatgatcga
     3901 ctgaacatat tccatcgatt ctcccgccgg atcggtgtcg tccagccgaa gattgcaggt
     3961 tcccttataa tccctggcga tgccgaaatt gaggcatacg gatttggcat gaccgatatg
     4021 aatatagccg ttcggttccg gcggaaaacg ggtcgcaact ttcccgttgt ttttgttcgt
     4081 tttcagatcg tcattgatga tgtcgcggat aaaatcggtc ggggttgagg ctgtctcgtc
     4141 tgtcatggga atattcaaac tccttcagtt ccataaaaaa tacgctctgg acctataata
     4201 tttactgaaa tatagcaaga gcggagcatg caaaacaaca catgcatccg ccgttgatgt
     4261 tttcaaaaat gcttgctttc agtcgaaaaa tcgaatcaat gttcggccgg ctctatcctg
     4321 ccctgcgtca tgattttcgt cagaacccag gccatgttgt aggctgcgct ttgcattctt
     4381 ccgctgaaac tgccccaggc gctggtaaag ggatttccgg aaaacgtgct gacattcacc
     4441 tcgcccgaga ataatttctg cccgtcggcg gcatcggtaa acgttatttt gcagacatat
     4501 tcatcaggcc ggttcgtcca ggcgttccag ttcagattaa ttttggtcac ggccacttgc
     4561 gcaatcacac cctgggtgat cttgtcgcca ccgctcacca cggatatctt tttgttggtg
     4621 atattggctt ccttcaacag aaccgggaaa gcatcggcaa atgccttggg aactgcactt
     4681 ttatcggcat cccattccgc gttatttgta tagcccagat cctttggatt gatggcgctg
     4741 taatcgatgg gcagaacgct gaaatccgtg ccggccttga tcttttcgga agccgccggc
     4801 ggctgttgca ctgttgtata ataaacaccg gcatccgccc atgatgcgca cagcagaaca
     4861 cacatgccga caaacattga gatgatttta aaataacctt tcattttctg cctcctctga
     4921 aaatcgttta atgtttcaat aatgcatgaa ctttctttcg aatgcatttt taccagttat
     4981 acgttcattt ttctattctt ttttccattt tttctgacgt ccccccggca gggatttttt
     5041 tactcatttt ttttaaaaaa gctgatttgt aagccaggat atggtaaaaa tgagatgctt
     5101 attcctgttt ctgaaatgaa aaatattgac ggctggaacg atcatgaacg aaaaaaccgg
     5161 caacgcatcc tcgcccggct tttacggccg gccgacaagg caagacgggc agctatccgc
     5221 tgtaccggct gcacatttgc gtctgtccgc acatttcaga atggttatct tcattgccac
     5281 gttatgttta tccttctgcg cgctgaactg ccgggccggt gtggaacaaa atgatgacgt
     5341 cttgagaaaa aaaatggttg aggagcagct caaagccaga ggcataaaaa atccgcaggt
     5401 gctgcgggcg atgagcaagg tggaacgtca ccaatttgtc cctgaagccc tgcgcaaaat
     5461 ggcttacgcc gattccgctc ttcccatcgg ggaaggacag accatctctc agccatacat
     5521 tgtcgcttgg atgacagaag tgatcaaccc tcaagcggat atgaaggttt tagaaatcgg
     5581 caccggttcc ggctatcagg ccgcaattct ggccgaactc tgccacagtg tttataccat
     5641 cgaaattatt gaaacgctgg ccaaacgcgc cgaaaagatt ctcgggaaac attaccaaaa
     5701 tgtccatgtc aggaccggcg acggctatca gggatggccg gaggccgcgc cttttgatgc
     5761 cattctggtc acctgcgcgc ccaccaaagt gcctgcgcct cttgcgaaac aactcaagga
     5821 aggcggcaga atggtcattc ccgtgggaaa tgtcaaagcg caggagttgt atgttttggt
     5881 caaacgcaat ggacgccttg aacagcaggc gatcatcccg gtccttttcg tgcccatgct
     5941 ggacaaggag ggggaaaaat actgatcgac gtgatgagca tctgtgccag gtattcaccg
     6001 gcatttccgt ctttgatgtt taaaacctga aggctgacga atcccttggc atctgccggt
     6061 cagacttatc ttgcgcgcgg cggttcttct tccagcatgt cgtacaatat tccggcgaca
     6121 ccgccgacaa ttttttgaca gatgatctga tgatgctttc tttcggttac gtcggcggtt
     6181 gtagcatgtt ttttcagcaa ctcggaacaa caactgctgc cgtgaagatc ttcaatcttc
     6241 cggttcaacc cccgcacttt ccgataggcg gtcagtttgg tttccatatc gttgggatcg
     6301 gcgttgccgt atgccagtcc cagcaccatg aagccgccgg taacagcgcc gcatttttcc
     6361 cgcagtctgc ccatgccgcc gccgaaggtg gaggcaatcc gcttcgcttg cttttcatcc
     6421 aggccgaacc ggccggcaaa agccagcaag acggattgag agcaggcgta tccctcgttg
     6481 aaaaaagaca cggcgctgtt gattgtttcc tgtttggtca tgaatgtatt ttcggacaga
     6541 cgattggttt gtccccgcgt cagggatatc cgaagaaatg ctttttgtca aaaatttaat
     6601 cggaagcagg aaccccgttt gattccggca atcaagcgcc cttcccgtcc gctgagagcc
     6661 gatatccatg cgactccttt tcctctgaaa ggcgtcgaat gcgaacggcc aacaagaatc
     6721 cttagtgaag cgcaacatta attgcttgtg ttttatgcaa atctgtgaat gagattcaac
     6781 aggttaaaaa tcgaatttca ggataggatt ttatgaatgt caaaacccag gagttgttta
     6841 atttttattc acatttggcc ggaatgatcg cggccgttat cggcaccgtg tatctggcca
     6901 tcgaagccag tttctcggca tccggtctgg tcactgctct gatttacggg atttccgttg
     6961 tttttctttt ttcagccagt tcgctatacc acgccttcaa aaagaaggaa aacgagctgt
     7021 ccttctggcg aagaatggat cgtctggcga tctttttcat gattgcgggc acatttactc
     7081 cggtctgcta tttttgcctg gagggcaacc aacgatgggc gatgattgct tttcaatgga
     7141 gccttgtcgg ggttggcgta atctctcaga tttttttccc gcgcgcgccg cggacacttt
     7201 acgcgatgct ctatttattc atgggatggt ccggactctt cattattaaa caggtcctgg
     7261 ccagtatgtc tatttcccag acggttctgc tgtttaccgg cgccggcgcc tttacggcag
     7321 gcggcattat ttatgccatc aaaaaaccga aattgattcc gggcattttc agttttcatg
     7381 aacttttcca catcctggtt ctgattggtg gagctctgca ttacgccatg atttacggcg
     7441 tttatgcgca gctcggtgcc tgagcgatac acaaatgcaa tgtacacccc ctgacacggc
     7501 cgcggcttgc cttctggcag ccgaagcgga tttattcctc aaagagtatc aacagcatta
     7561 ttctgccggc gaaacaaagc ctcaacagat cggcatgccg ttttttctgc acgcgcccgg
     7621 atccgatacc ggcgtgcttc tgattcacgg cctgatggcc gcgccggaag aagtcaggga
     7681 gtgggcggag tttctgcacc aaaaaggtct gaccgtttat gcaccgaggc tcgccggcca
     7741 tggaacatct tgcaaagatc tttcgacacg ccgctatgaa gactggctgg attcggcgga
     7801 gcgcggccat gccattttga aaaaatactg cgacaaaatt ctggtggccg gtttttccac
     7861 cggggcgggc cttgcactgc aatccgtcat tttaaaaccc catgattttg aagccgtgat
     7921 cgccgtcagc gcaccgctga aattcaaaag cttttcgtcg aggtttgcgg aagttctcaa
     7981 cgcgttcaac cgattctgcc attcccgggg cgttaacacc cttgccagag actacatcaa
     8041 aaacgacgcg gataatcccc acatcaacta tctgctctgt ccggtgagcg catttgttca
     8101 ggtaaaaaag ctgatgaaaa aagtcaaccg gtcgcttccg gacattcgca ttccggcgct
     8161 ggtgattcag gcgaaaaacg atccgaaagt cgcgccgcaa agcggtcctg aaatattcag
     8221 acggctgggc acaaacaaaa aacgttttgc ctggatcgat catgatctgc acggcattgt
     8281 tcgcggcaac atttccggta tggtctttaa ggaagtggaa ttattcctcg cggagcagaa
     8341 tctcattcat atccggcctg aaatgagcaa tcgtttgccc ggagcataaa cgctgtaacg
     8401 tcctgcatta tatgatgaaa tcgactcttc ccgcacacta taacttgaga aaagaatttg
     8461 ttttgtaaat aaagcttgac tattcctccg cgataccgta aggtcgcgcc aacttcagat
     8521 ccacaagatt gagatttgaa ggaaagagtt gaatttagac attttgaagc acataggtta
     8581 gccgccgggt actttagagt cttggtggct tttttagttc tgtcatcgtg ctgattcgac
     8641 ttttgaaaat ttatgaaagg aggatgagaa ctatgtcaga aggtaaagtc aaatggttta
     8701 acgaaagcaa aggcttcggc ttcatcgaaa aggaagaagg cggcgatgta ttcgtacatt
     8761 tcagctcgat tcagggtgaa ggtttcaaga ccctaaatga aggccagcgc gtaagttttg
     8821 acgttgcgca gggcaaaaaa ggtcaggaag ccacgaacgt cannnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8941 nnnnnnnnnn nnnnnnnnnn nngagctccg aaagcggtgt cgatctgaac cgttccagat
     9001 tgtctcagat caaaggaatc agttttctgg atgacggcgg cggctacgtt tttaccggca
     9061 cgcgcccgcc catttccgat ttggacggcc tggccgattg cgacctgaca ttgatcaaat
     9121 atctacaaaa aagatggagc tatatcccca tcaaccgggg ccgcggctgc aactggaatt
     9181 gcgaattctg cgtcgtgaac aaacaatacg gaaaatacaa ggctgcgtcc atcgatacgg
     9241 cgctgaggca actgatccga tatgccgata tgggatacac taaattcttt ttcaccgatg
     9301 ataattttgc acagcatccg gacgaggcca ttgctctgtg caaagccatc ggcaattata
     9361 aacgaaattt tttgaaaaaa atcgatttgg tggttcaggt gcgttcggaa gtggcggaaa
     9421 atgacgccct catcgaggcc atgcattttg ccggtgtcag catgctggcg atcggttacg
     9481 aaagcccgat caatgaagaa ctccgggcca tgcgtaaagg cgtaaccgtg gagaagctga
     9541 ttgcccgttc ccgaaaactc tccaatgttt tttatctgca cggcatgttc attttcggct
     9601 atccgctcaa cgacgatgca caggggccat cttctttcac cgtcaaacaa cgggcgaaaa
     9661 tctacaaaga tttttttaaa aaatccggta tcgataccat tcaggtattg aatgcggttc
     9721 cgcttccggg ttcggatctc agaacaagac tggaacggga aggccgtctt ctgcccctga
     9781 aaatagtggg ctgggataaa tacgacgggc ttttcctctg ctacgatccc cggcccgaca
     9841 gtatggacgc atttgaactg cagggcatgc ccggcctgct gatgaaaaaa aaatatctcg
     9901 gaggatttat caaacggaat ttgaattacg gcaactggct gaactggacc tatattgcca
     9961 caatcggatt tcccattcaa ttcgtcgaat tttacatccg ccggtttatc cgtaatttgg
    10021 cggaaagaca acgtgccggt aaacggcccg acggcgcctg gagtccgcgg acgatttttg
    10081 ccgttccgct gatcagcgcc tggaaagata tcaaaagaat atggagaaat ctgtttgtca
    10141 ggacatacgc gggcgccatc gtcaaccgct ggttaaaaat ttatcgcaaa agccgttacg
    10201 gagacaaact ccggaaattt ttcctggaca gcaagataac cccaaagagc cgccatcgcc
    10261 tcaacacctg aaaaaaatgc ttaaattcat ccgttgatta aaatgatctt gctctcttgt
    10321 cggacaatga ttaaatttct tttcaaggtg aaatgatgaa aaacaaatac acgcccgtct
    10381 ttattcttgt catgcttttt ggattcatat gggtatattt aatggcgcaa gttacgtccg
    10441 aacgggtgca gggagggaga atcatggata aaaaaatttc gggacaggat gccgaaaaaa
    10501 gaatgacgcc gctccagcgc gatgtcgcct tcggcaacgc cacagaaccg ccttttcaaa
    10561 acgaatactg gaacaatcat caggaaggca tctatgtcga tattgtttcc ggcgttcccc
    10621 tgttcagttc gaaggataaa ttcgattcca aaaccggctg gcccagtttt acaaaaccca
    10681 tcaaggacaa cgccgtctgg agaaagaccg atttcagcca ctccatggta cgcacggaag
    10741 tcagaagtac gcaagcggac attcatctcg ggcatgtttt tgacgacggg cccgccccca
    10801 ccggcaggcg atattgtatc aactccgcat cattaaaatt tattccccga gagaaaatgg
    10861 aggcggaagg ctacggtgag tacttgtcgt tatttgctgc tccatccggc aaatcgaccg
    10921 aaacggccat ttttgccgcc ggctgtttct ggggcgtgga ggagtatttc agccgggtaa
    10981 aaggcgtcgt caacagcgaa tcgggttaca ccggcggaac cgttaaagcc ccctcttatg
    11041 aacaggtctg cagcggcaaa accggacatg ccgaagctgt ccgcgttacc tatgatccga
    11101 caatcgtaac ttatgaacgg ctgctgcaac acttctggga attgcacgac cccaccagcc
    11161 ttaacaggca gggcaacgat gtgggcaccc aataccggtc ggcgatattt tataccagtc
    11221 cgcaacagga aaaagcggca agagcctctg cagaaaaact tgcccgatcc ggcagatata
    11281 ccagaaaaat cgtcacgcaa attcagcctg caaaagattt ctatccggcg gaagattacc
    11341 atcaggacta cctgaagaaa aatccccgcg gctactgtca
//
LOCUS       DAOOLB010000069        11370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_6390_length_11370_cov_50.162439, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000069 DAOOLB010000000
VERSION     DAOOLB010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6390_length_11370_cov_50.162439"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /gene="thrC"
                     /locus_tag="PLF77_08445"
     CDS             complement(<1..1253)
                     /gene="thrC"
                     /locus_tag="PLF77_08445"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HPC08919.1"
                     /translation="MPIRYYSTNRHLKNVAGITPFTGKVSFREALLKGQASDEGLFMP
                     DTIPQLAITDIQQLNGKPYRDTALLVAKAFLADEFPPDILQQVVTESYNYPVPLEEVR
                     PDHFVMRLDRGPTASFKDFAARMMARLMSHCRPKGERLNILVATSGDTGSAVGEAFKG
                     VTGIDVTILYPENEVSGMQKKQLDTIGQNVQALSVAGKFDDCQNLVKQAFSDPRNEKF
                     NLTSANSINFGRILPQIIYYVYAYAQLAQNKEEAIFSVPSGNFGDALGCEYARRMGLP
                     VAKLVMPTNENDEFPIFMATGKYNKVSPSRACISNAMNVGHPSNLARFFDLYGGTVDR
                     VGQVHKYPDLEEMRRHIFSVSVSDSQTRETIKNVYKRYQAVLEQHGAVGWAGLEEYLK
                     TTPEQKLAICLETAHPAKFPEEIEKL"
     gene            1430..2650
                     /locus_tag="PLF77_08450"
     CDS             1430..2650
                     /locus_tag="PLF77_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructo-2-kinase/fructose-2,
                     6-bisphosphatase"
                     /protein_id="HPC08920.1"
                     /translation="MSDKKLYIVLMGLPARGKSTLAIRLRDYFLKEGIPTRIFNNGNL
                     RRLYLPHLETSSADFYSPENETAVALRKKFARMNMDRARKYLFNNGMIAILDATNAGR
                     ERRGLIEKTLNDHDLLFIECVNDDDDILKMSIQEKIRLPEFAYLKDKATREFLKRIDY
                     YKAIYMPLKAERNYIRIDTLQKMIVEEKHSDVIPYYERLRDYLVTDEVKNLFLIRHTE
                     TEYNVENRIGGDPALTPKGLKQASALGRFFRKKKISYIFTSPKQRTIHTAKLIRSQQD
                     DCRIIPLKEFDEINAGICERMTYEDIEQKMPHVFKKRGADKYNYIYPEGESYALMKPR
                     IMEGIKKAFFLNRHANSIMIVGHQAVNRMILSYFLYRREDDVPYIYIPQDRFYHIVSK
                     QDKKIFELKRYDSD"
     gene            2778..3830
                     /gene="selD"
                     /locus_tag="PLF77_08455"
     CDS             2778..3830
                     /gene="selD"
                     /locus_tag="PLF77_08455"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2829..2831,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPC08921.1"
                     /translation="MEEKKGVRLTETVQSAGUACKIGPADLSEIMRDLPLIHDPNLLS
                     GFEHAEDAGVYKISADMALVQTVDFFTPTVDDPFTFGQVAAVNALNDIYAMGAKPLTV
                     MNIVCFPIKTMAVSVLRNVLQGGLNKIHEAGALLIGGHSVEDNEIKYGLSVTGVAHPD
                     SILYNRGARAGDKIILTKALGTGIISTAIKAGEASPKQIQQVTVTMTTLNKKSAQLMI
                     AAGDIHACTDITGFGLLGHACEMIEGSDVGLRIHSAAIPALDGVRDLMETGFVPGGLY
                     RNKDFRIKQVVKEKTCPDWLFDLLFDPQTAGGLFFSVPGDKAQNLAEKMRGAGIADAA
                     VVGEVVPNHPGTILVY"
     gene            3864..4895
                     /locus_tag="PLF77_08460"
     CDS             3864..4895
                     /locus_tag="PLF77_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08922.1"
                     /translation="MKVNAIPIKKETELNFHKLMDMVQPDDSLLLLMYGSPDPDAIAS
                     AMALREILQRTKGLSKSAFVSTEPLDRQQNIELVSCLHLDIQRMNQVDVASYRVTALL
                     DAQPSFFTHFPDALRPQIVFDHHPYEGQWSAELMDVRPNYGALSSILTEYLLCSRIKI
                     PRNLHTALLYGIKTDTDNFDRATIIEDISAYAYHTKYANMPLIRRIELNQTPDRFLKY
                     FHYAYHHMNNFRGRRICFLGNVESADVCVQIADFYLHLIGTYYVVVTGIVQDKLIIIF
                     RGDGYRQNCGAIAQKAFGAVGKGGGHRSAARMEIHLSALKEILGGELSQKNVERFLFS
                     SLRREQKTT"
     gene            4938..5981
                     /locus_tag="PLF77_08465"
     CDS             4938..5981
                     /locus_tag="PLF77_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08923.1"
                     /translation="MRDLGFKKEITTGYYLDPFNGFQEKKITIESRYDEITGVASRIL
                     PYRMRIAQKPDMNEYLEKSPESLCPFCSDLFDKTTPRFIPSVMEDGNFKRGEACLFPN
                     VFPYDQNNNVAIFSSRHLVAMNELTPEFMRDGFLVCRDYFHRVVELNLGYKYCSINWN
                     YMPPAGGGLIHPHLQTIAGYRPTSFMQKLLSSAQNYAANSNGENLWSNLIALEKKADK
                     RFIADTGVVCWLTSFAPKGMAGEIDFIFREKTSFFDLTESNFSEFLAGLSRVFSYLYT
                     NNFISFNLSLYATMNNNNYFCVQGKIVPRFVIYPLGTSDLNYFEKLHNEIICPTVPEE
                     LCRELQPYFKSLL"
     gene            6119..7219
                     /locus_tag="PLF77_08470"
     CDS             6119..7219
                     /locus_tag="PLF77_08470"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HPC08924.1"
                     /translation="MLGIFPQDDHNQTLRLKRFFMALGSYLMWFAICVMFYAQGQMTR
                     GNIQVLVISSLGILFCNVLIYAMIRSGYNKRFKDPSLTLLQMIIATFWVMVIAYYADT
                     TRPLVLLTYLVVFIFGLFRLSVWQFLFLSAFAVVNYSLVIFLLYNINPDLVSTSDVLS
                     IVVLALVLPWFSLVGGYITRLRARISHAFSTIEKLAIIDDLTQVFNRRQMFKILEYQK
                     ALVDRGIHPFSICIFDLDHFKRVNDTYGHVAGDFVLKTVAQETQKNLRNIDHIARYGG
                     EEFILILTNVEKSQAILCADRIRNLVKKIVFENMPDDFKMTISVGVTEYQAGEDIQQA
                     INRADTALYRAKTNGRDRVEYEPMSKDKGEKG"
     gene            complement(7399..7671)
                     /locus_tag="PLF77_08475"
     CDS             complement(7399..7671)
                     /locus_tag="PLF77_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HPC08925.1"
                     /translation="MPIYEYKCQKCGKQFEAFQGITDPELKTCKFCKGKVHKMMSLSS
                     FSLKGSGWYVTDYKGKKPGAAPAKGKTETTDSAAKTETSKTESSES"
     gene            complement(7690..8193)
                     /locus_tag="PLF77_08480"
     CDS             complement(7690..8193)
                     /locus_tag="PLF77_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08926.1"
                     /translation="MTKKAFNDAENLVSRQFRLSDADFRKNRYEVKTLAYLDEQEIKD
                     GTFAHLCKYTYERPDGLANRSEKVFNFYRICLQDNIILDAVERANPFIKLSPLMLYIA
                     VHELIHILRFASGEIKFDADAKEKEKEEVIVNNLTRAALLPVKGYDLEIVLDCFSSHF
                     KNFDSFN"
     gene            8430..9569
                     /locus_tag="PLF77_08485"
     CDS             8430..9569
                     /locus_tag="PLF77_08485"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002528.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HPC08927.1"
                     /translation="MAGKTGLKETAKIRIGVLYGGRSGEHDVSLCSAASVVAALDRKK
                     YKVTAIGIDRDGRWYVQEKPEIIPDKDFGKKMALKKKGMWLVNHFEQRNKLHLYDIKN
                     KNKEVVVDVVFPVIHGTFCEDGTLQGLLELAMAPYVGADVTGSAVGMDKDIAKRLLCE
                     AGIPVVPSVTVNKQDWKENNRVIMKQALSGLGLPLFVKPVCAGSSVGVRKVKKEEELA
                     KAMNFAFQFDTRVMIERAIDCREIECAVLGNENPAGSVLGEIIPNHEFYSYEAKYIDP
                     DGAALKIPAEINKTLSDKIRKIAVEGYMALGCTSMARVDFFLDKKTNKFYLNEINTLP
                     GFTSISMYPKLWEATGLKYSILLDKLITLAIDRHTKKLEIKTEGI"
     gene            9573..10025
                     /locus_tag="PLF77_08490"
     CDS             9573..10025
                     /locus_tag="PLF77_08490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HPC08928.1"
                     /translation="MVLDVIENLHKYSALNQGFARAIDFLLRPDLRNLALGKYEIDAE
                     RVYAMVSKDSGCKKEEALLERHEKYIDIQLVLSGTDTMGWKPKSLCKQPTGAYDEGED
                     IQYFADEPDSWIATRSSSFVIFFPEDAHMPLISSGQLHKVVVKITTSW"
     gene            10087..10278
                     /locus_tag="PLF77_08495"
     CDS             10087..10278
                     /locus_tag="PLF77_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08929.1"
                     /translation="MKRPASLVVGILLFVMAVAQAGRFIIGLNVTAEGVEIPVWISAV
                     AAVALGALGIWLLKERTRQ"
     gene            10353..10775
                     /locus_tag="PLF77_08500"
     CDS             10353..10775
                     /locus_tag="PLF77_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4019 domain-containing protein"
                     /protein_id="HPC08930.1"
                     /translation="MIRKILCFVVIGIFLGVTAVVADSSDREKAAIASAEKWLKLVDQ
                     GKYIESWKESADYFKRAVSQEQWEQAMKSGREPLGKLIFRKVTNALYTTSLPGAPDGQ
                     YVVIQFNTSFENKKSGIETVTPMVDKDGRWRVSGYYIK"
     gene            complement(10793..>11370)
                     /locus_tag="PLF77_08505"
     CDS             complement(10793..>11370)
                     /locus_tag="PLF77_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08931.1"
                     /translation="AVLSKDYLHVSMHVQYKKTLRDNIFMIGCASSHSQLNALFKDPR
                     CEYRWLLNDGGNLSGKRDFTVERVRIDNEDVPIIETKSTKRGYEVWCGSDRLKKKINQ
                     DVKLEMDISTKQARQKRTFPVYLVYPVRGLEIKFDYANAGIKNVREESFFAGKHPQPS
                     VTGKKGKSIAVKISDEEWIFPTSGVIFIWDD"
BASE COUNT         3037 a         2543 c         2780 g         3010 t
ORIGIN      
        1 agcttttcaa tttcctccgg gaattttgcc ggatgggctg tttccaggca gatcgccagc
       61 ttttgttcag gtgtggtttt taaatattct tccaggcccg cccagcccac agcgccatgc
      121 tgttccagaa cggcctgata gcgtttataa acgttcttga tggtttcccg ggtttgcgaa
      181 tcgctgacac tgaccgaaaa aatatgtctg cgcatttctt ccagatcggg atatttgtga
      241 acctgtccaa cgcgatcaac agtgccgccg tacaaatcaa agaaacgcgc cagattactg
      301 ggatgtccga cattcatcgc gttggaaatg catgcgcggg agggagaaac cttattgtat
      361 tttcctgtcg ccatgaaaat cggaaactcg tcattttcat tggtgggcat gaccagtttg
      421 gcgaccggca aacccatgcg gcgggcgtat tcgcaaccca gcgcgtcacc gaaatttccc
      481 gacggaacgg aaaaaattgc ctcttctttg ttttgggcaa gctgggcata ggcgtaaacg
      541 tagtagatga tctgcgggag aatcctaccg aagttaatgg aattggccga ggtcagatta
      601 aacttttcat tccgcggatc ggaaaaagcc tgcttgacca gattctggca atcatcgaat
      661 ttgccggcaa cgctcaacgc ctgcacgttt tggccgattg tgtcgagctg ctttttctgc
      721 atgccactga cttcattttc gggatacaag atagtaacgt cgattcctgt tacgcccttg
      781 aaggcttcgc ccacggcgct gcctgtatcg cctgatgtag ccaccagaat gttaagcctt
      841 tcgcctttcg gacggcagtg gctcatcagc ctggccatca tgcgtgcggc aaagtcttta
      901 aatgacgctg tcggtccccg gtccagccgc atcacgaaat ggtccggcct gacctcttcc
      961 agcgggacag gataattata cgattcggtc acaacctgtt gaaggatgtc cggtggaaat
     1021 tcatcggcaa gaaacgcttt ggcgacaagc agtgccgtat cgcgatacgg ctttccattc
     1081 aactgctgaa tatcggttat ggccagttga ggaatggtat ccggcataaa caagccttca
     1141 tcggaggcct gacccttgag cagggcttcc ctgaaagaca ccttgccggt aaagggggta
     1201 atccccgcaa cattttttaa atgacggttt gtactgtaat aacgaatagg catacttttc
     1261 ttcctgctgt gcttggttaa gtctttaaac ttatttggat taaaaaatca attcaaaaaa
     1321 atgccgcgac ccttttatta tccattgaaa ggacatgata tccatgattg acataaaaaa
     1381 gtattgttcc tatacttcac acaatattat catggaaaaa caataaggta tgagtgataa
     1441 aaagttatat attgttctaa tgggccttcc cgcgcgggga aaatcaacgc tggccatacg
     1501 tctcagggat tattttctca aagaagggat tcccacccgc atattcaaca atggaaattt
     1561 gcgcaggctt tatctgccgc atctggagac atccagcgcg gatttctatt ctcccgaaaa
     1621 cgaaacggcc gtggccttga gaaaaaagtt cgcacggatg aacatggaca gagccaggaa
     1681 atacctgttc aacaacggga tgattgcaat tctggatgcg acgaacgcgg gaagggaacg
     1741 gcgcggatta atcgaaaaaa cgctcaacga ccacgacctc ctgtttatcg aatgcgtcaa
     1801 cgatgatgac gatattctga aaatgagcat tcaggagaag atacgtcttc ccgaatttgc
     1861 ttatctgaag gacaaggcga cccgcgagtt tttaaaaaga atcgattatt acaaagcgat
     1921 ctacatgcct ttaaaagcgg aaagaaatta tatccgcatc gacacgcttc agaaaatgat
     1981 tgtcgaggaa aagcacagtg acgtgatccc ctattatgaa cgtttgcggg actatctggt
     2041 cacggatgaa gtaaaaaatc tttttctgat tcggcacacg gaaacggaat ataatgtgga
     2101 aaacagaatc ggcggcgatc cggcattgac accgaaaggt ctcaaacagg ccagcgcgct
     2161 gggacggttt ttcaggaaaa agaaaatttc ttatattttc accagtccaa aacaaagaac
     2221 gattcatacg gcgaaactca tccgttcgca gcaggacgac tgccggatca ttccgcttaa
     2281 ggaatttgat gaaatcaatg cgggaatctg cgaaagaatg acttatgaag atatcgagca
     2341 gaaaatgcca catgttttca aaaaaagggg agcggacaag tacaattaca tttacccgga
     2401 aggcgaaagt tacgcgctga tgaagccccg catcatggaa ggcattaaaa aggctttttt
     2461 cctgaaccgg cacgccaaca gcattatgat cgtcggccat caggcggtaa acagaatgat
     2521 tttgtcgtac ttcctttatc ggcgcgaaga cgatgtcccc tacatttata ttcctcagga
     2581 ccgtttttat catattgttt ccaaacagga caaaaagata tttgagctga aaagatacga
     2641 ttcagactga aggttatttg accaaagact gaacggtgtg atgaagattt cgttgacatg
     2701 cagaagtgtt gtccttatac tttcaacgtt atttcgtgaa tgtttggaaa attttgtaac
     2761 cgattttaag aggaaaaatg gaagagaaaa aaggtgtccg tttaaccgaa accgtgcaga
     2821 gcgccggttg agcctgcaag ataggtccgg cggacttatc cgaaatcatg cgcgatttgc
     2881 cgctgattca cgatccgaat ctgttgagcg gatttgagca cgcggaagac gccggagttt
     2941 ataaaatctc cgctgatatg gctctggttc agaccgttga tttttttacg cccacggttg
     3001 atgacccgtt caccttcggc caggtagctg ccgtcaatgc gctcaacgat atttatgcga
     3061 tgggtgcaaa acccttgacg gtcatgaaca tcgtttgttt cccgattaag accatggctg
     3121 tttcggttct gcgaaacgtt ttgcagggcg gcctgaataa aatccatgaa gccggtgccc
     3181 tgctgatcgg cgggcacagc gttgaagaca atgaaatcaa atacggatta tctgtgacgg
     3241 gagtcgctca tcccgacagc attttataca atcgcggcgc cagagccggg gataaaatca
     3301 ttttaaccaa agctctcgga acgggaatta tcagcacagc gatcaaggcc ggtgaagcat
     3361 caccgaagca aattcaacag gtaactgtta ctatgaccac gctcaacaaa aaatcagcgc
     3421 aactgatgat tgccgccggc gatattcacg cctgcaccga catcaccggt ttcggcttac
     3481 tgggtcatgc ctgtgagatg attgaaggaa gcgacgtcgg tctgcgcatt cattcggcgg
     3541 caatacctgc tctggacggt gtacgcgatt tgatggagac gggtttcgtt ccgggcggcc
     3601 tttatcgcaa caaagatttc cgcatcaaac aagtggtgaa agaaaaaacc tgtccggact
     3661 ggctttttga tctgcttttt gatccgcaga cggccggcgg actgtttttc agtgtgcccg
     3721 gcgataaagc acaaaacctt gccgagaaaa tgcgaggtgc gggaatagcc gatgccgccg
     3781 ttgtcggaga agttgtcccg aatcatcccg gcacaatatt ggtttactga ggaggatcaa
     3841 ccggccctga agacgccggg accatgaaag tcaacgccat cccgattaaa aaagagacgg
     3901 aacttaattt ccacaagctg atggacatgg ttcagccgga cgattcgctg ctgctgttga
     3961 tgtacggcag tcccgatccc gatgccatcg catcggcgat ggcgctgcgc gaaattcttc
     4021 agcggaccaa gggactttcc aaaagcgctt ttgtttccac ggaaccgctg gaccgtcagc
     4081 aaaatatcga gttggtgtcc tgtctgcatc ttgatattca acgcatgaat caggtggatg
     4141 ttgcatcgta ccgtgtgacg gcgctcctgg atgcgcaacc gtcgtttttc acgcattttc
     4201 cggatgctct ccggccgcag attgttttcg accatcatcc ttatgaaggg cagtggagcg
     4261 cagagctgat ggatgtgagg ccgaattatg gggcgctgtc cagcatcctg acggaatatc
     4321 tgctttgctc ccgcattaag attccccgca acctgcacac ggcattgctc tacggcatca
     4381 aaactgacac ggacaatttt gatcgggcca cgataattga agacatcagt gcgtacgcgt
     4441 accatacaaa atacgccaat atgccgctga tccggcgcat cgagctcaat caaacacccg
     4501 accggttttt aaaatatttt cattacgctt atcatcacat gaacaatttt cgcggtcggc
     4561 ggatctgctt tctgggcaat gtcgaaagcg cggatgtctg cgttcaaatc gccgattttt
     4621 atctgcactt gatcggtacg tattacgttg tcgtcaccgg aattgttcag gacaaactga
     4681 ttattatttt ccgcggcgac ggttacagac agaactgcgg ggcaatagcg caaaaagctt
     4741 tcggagcggt cggcaagggt ggcggacacc ggagcgccgc acgcatggaa atccatctga
     4801 gtgcgttgaa agaaattctc ggcggggaat tgtctcaaaa aaacgttgaa cggtttcttt
     4861 tcagcagttt aaggagagaa cagaaaacaa cttgaaataa aaatcaaaac cgacctgtgt
     4921 tgaaagaaag gaattctatg agagatttgg gatttaaaaa agaaataacc accggatatt
     4981 atcttgaccc gttcaacggt tttcaggaga agaaaattac catcgagtcg cgttacgacg
     5041 agatcactgg cgtggcaagc cgtattctgc cttaccggat gcgcattgcc cagaagccgg
     5101 atatgaatga atatctggaa aaatctccgg aatctctgtg cccgttctgt tccgatcttt
     5161 ttgataagac aaccccccgt tttattccct ctgttatgga agacggtaat ttcaaaagag
     5221 gagaagcctg tctctttccg aacgtctttc cctacgacca gaacaacaac gtggcgattt
     5281 tttcctcgcg tcatctcgtg gcgatgaatg aactgacgcc ggaattcatg cgggacggtt
     5341 ttctggtttg ccgggactat tttcaccggg tggtcgaatt gaatctggga tacaaatact
     5401 gttcgatcaa ctggaactac atgccacccg ccgggggcgg tctgatccat ccgcatttgc
     5461 agaccattgc cggttaccgt ccgacaagtt ttatgcagaa acttttatcc agcgcgcaaa
     5521 attacgccgc gaattccaac ggagaaaatc tgtggagcaa tttaatcgcg ctggagaaaa
     5581 aagccgacaa gcggtttatt gccgatacgg gtgttgtctg ctggctgacc agttttgccc
     5641 cgaagggcat ggccggggaa atcgatttca tttttcggga aaagacctcc ttctttgatc
     5701 tgaccgaatc caatttcagc gagtttctgg cagggctgtc ccgtgtgttc agctatcttt
     5761 ataccaataa cttcatcagt tttaacctgt ctctctatgc caccatgaac aataataatt
     5821 atttctgcgt gcaggggaaa atcgttccgc gtttcgtgat ttatcctttg ggaacgagcg
     5881 atttgaatta ttttgaaaaa ctgcacaatg aaatcatttg tccgactgtt ccggaagaat
     5941 tgtgccgtga actccagccg tatttcaaaa gcctgctgta aatcgtctgc tccgccagga
     6001 ggatgtttca taagaaaaat tccgcaatga tttcaatgct tgacagcatg atgttttgcg
     6061 gattatataa taacgaaatg aaacgtttct gaatattgga agcagaaggg agatgcgaat
     6121 gcttggtatc tttcctcagg acgaccacaa tcagacgtta cgattgaaac gttttttcat
     6181 ggcgttggga tcttacctga tgtggtttgc tatttgcgtg atgttttatg cgcagggaca
     6241 gatgactcgg ggaaacatcc aggtgcttgt catcagttct ttgggcatat tgttctgcaa
     6301 tgttctgatc tatgccatga tcagaagcgg ttataacaaa agattcaaag atcccagtct
     6361 gacgctcctt caaatgatta tcgccacctt ctgggtgatg gtgattgcgt attacgcgga
     6421 caccacgagg cctttggttc tgctgaccta tctggtcgtt tttattttcg gacttttcag
     6481 attgagtgtc tggcagtttc ttttcctttc tgcctttgcc gttgtcaatt actccctggt
     6541 gatctttctg ctttataaca ttaatcctga cctggtcagc acatcggatg ttttgagcat
     6601 tgtggtgctt gcgctggttc ttccctggtt ttcattggtc ggcggctaca ttacccggct
     6661 cagggcgcgg atatcacacg ccttttccac cattgaaaag ttggcaatca tcgatgatct
     6721 gacgcaggtc ttcaacaggc gtcagatgtt taaaatcctc gaatatcaaa aggcccttgt
     6781 cgatagaggt attcatccct tctccatttg tatattcgat ctggatcatt tcaaacgcgt
     6841 gaatgataca tatggacatg ttgccggaga ctttgtcctg aaaacggtgg cgcaggaaac
     6901 ccagaagaat ttacgcaaca tcgatcacat tgcccggtac gggggcgaag agttcattct
     6961 aatcctgact aatgtggaga aaagtcaggc gatcctttgc gcagacagaa taaggaatct
     7021 ggtcaagaag atcgtgtttg aaaatatgcc cgacgatttc aaaatgacga tctccgtggg
     7081 tgttacggaa tatcaggccg gcgaagatat ccagcaggcg atcaaccggg cggatacggc
     7141 gctttacaga gccaagacta acggcagaga ccgggtggaa tacgagccga tgtcgaaaga
     7201 caaaggggaa aaggggtgag taaaaaatct cctccacata atttaaaacc cgataagcga
     7261 cgcttatcgg gttgatttgt gttggccgtt tcgcggatga ttatatgaat tcgttttgct
     7321 gacggtcctt gaacaaaatt caatcaaaag atgcaaaggc gctgtgcacg aaaatcatca
     7381 aaaaagattt ccgtatgttt agctttcaga ggattcggtt ttagatgttt ccgtttttgc
     7441 cgcgctatca gtagtttccg tttttccttt tgcaggcgcc gctcccggtt tctttccttt
     7501 gtagtcggtg acataccagc ccgaaccctt caggctgaaa gaggaaaggg acatcatttt
     7561 atgcactttc cctttgcaga atttgcaggt ttttaattcc ggatcggtga ttccctgaaa
     7621 tgcttcaaat tgtttcccgc atttctggca tttgtattcg taaataggca tattaaaaac
     7681 ttacctccgt taattgaatg aatcaaaatt tttaaaatga ctgctgaagc agtctaaaac
     7741 gatttccaga tcgtagcctt tgacgggcag cagcgccgcg cgggtcaaat tgttgacaat
     7801 gacctcttct ttttcctttt ccttcgcatc ggcatcgaat ttaatctcgc cgcttgcaaa
     7861 acgtaaaatg tgaatcagtt catggaccgc gatgtagagc atcagcggac tgagtttaat
     7921 aaacggattg gccctttcca cagcgtccag aatgatgttg tcctgtaaac agattctgta
     7981 aaaattaaag accttctcgc tgcggtttgc caggccatcc ggtctttcat acgtatattt
     8041 gcaaagatga gcaaacgtcc cgtctttgat ttcctgctca tcaagataag cgagcgtttt
     8101 tacttcatat ctattctttc tgaagtctgc gtcgctcaat ctgaactgac gcgataccag
     8161 attttcggca tcgttaaaag ctttttttgt cacgggcatt tgcgccgcgt taaaataaag
     8221 gggcataaat caccgcaggt attttcagtt cgtccgtaat atattgatag acactatgga
     8281 tgtcaagacc cctcgttcag gacgtctgct ggttaaaaaa agaaagtcgg aatcatcaat
     8341 cgcaaaatta aattgacaat tttatcgctc aaggcgtata actcttgaaa attataagac
     8401 aaataagcgc ttccatcgaa aggatcaata tggctggaaa aacgggtttg aaagagacgg
     8461 caaaaataag aataggcgtt ttatacggcg gccggtcggg ggagcatgac gtttcgcttt
     8521 gttccgccgc ttcagtggtt gccgccctgg accgcaagaa gtacaaggtg acagcgatcg
     8581 gcatcgacag agacggtcgc tggtacgtgc aggaaaagcc ggaaattatt cccgataagg
     8641 atttcggcaa aaaaatggct ctgaaaaaaa agggcatgtg gctggtcaac catttcgaac
     8701 agaggaacaa actgcatctt tacgatatca agaacaaaaa taaagaagtg gttgtggatg
     8761 ttgtttttcc ggtgatccac ggaacgttct gtgaagacgg cacgttgcag gggcttttgg
     8821 agctggcaat ggcgccttac gtgggtgcag atgtaaccgg ttcggccgtg ggcatggata
     8881 aagacatcgc caagagactt ctttgcgagg caggcattcc cgttgttcct tctgtcactg
     8941 taaacaaaca ggactggaag gagaataaca gagttatcat gaaacaagcg ctgtccggac
     9001 tggggctgcc gctttttgtc aaaccggttt gcgccggttc ttccgtgggc gttagaaaag
     9061 tgaagaaaga ggaagaactt gcaaaagcga tgaacttcgc ctttcagttc gacacacgcg
     9121 tcatgattga gagggcaatc gattgccgcg aaatcgaatg cgccgtttta ggaaatgaaa
     9181 atcccgccgg ttcggttttg ggcgagatca ttccgaatca cgaattttat tcctatgaag
     9241 ccaaatatat tgaccccgac ggcgcggcgc taaaaattcc ggcggaaatc aacaaaaccc
     9301 tttccgataa aatcagaaag attgccgttg aaggatacat ggcactgggc tgtacttcca
     9361 tggcgcgggt ggatttcttc ctagacaaga aaaccaataa attctatctc aacgaaataa
     9421 acacgctgcc cggcttcacc agcatcagca tgtatcccaa actctgggaa gccaccggtt
     9481 tgaaatacag cattctcttg gataaattaa tcacattagc catcgaccgc catacgaaga
     9541 aattggagat taaaaccgaa gggatataat ccatggtttt ggatgttatt gaaaatcttc
     9601 ataaatattc agccctgaat caaggattcg cccgagcaat agattttctc cttcgtccgg
     9661 atctgaggaa cctggcgctg ggaaaatatg aaatagatgc tgaacgtgtt tacgccatgg
     9721 tatcgaaaga ttccggctgt aaaaaagagg aggctctcct ggagaggcac gaaaaatata
     9781 ttgatatcca gttggttctg tcaggaacag acacaatggg atggaagcct aaatctttgt
     9841 gtaaacaacc aactggcgca tatgatgaag gagaagatat acagtatttt gctgatgagc
     9901 ctgattcctg gatagcaaca cgaagcagtt catttgtaat tttttttccg gaagatgcgc
     9961 atatgcccct gatatcgtcg gggcagctcc acaaagttgt tgtaaagatt acaacgtctt
    10021 ggtgagtgtt caaaggatct ttttattttt agaaaacagc aactttcttt ttcgggaggt
    10081 aaaattatga aacgtccggc atcgttggtc gtgggtattt tactttttgt catggcagta
    10141 gcgcaggctg gccgctttat catcggtctc aacgtgaccg ccgaaggcgt ggagattccc
    10201 gtctggatca gcgcggttgc cgctgttgct ctaggcgctc tgggtatctg gctgctgaag
    10261 gaaagaaccc gtcagtgaat ttacaacatt ttagaaaacg tcgaattcat gcaggaaatt
    10321 acgtctgtcg ctggatgaac aggaggaaat aaatgatcag aaagatatta tgttttgttg
    10381 tgattggaat ttttcttggc gtgacggccg ttgtcgctga tagctccgac agggagaagg
    10441 cggcgattgc atcggctgaa aagtggctga aacttgttga tcaggggaaa tatattgaaa
    10501 gctggaagga atccgccgac tattttaagc gggcggtttc gcaggagcag tgggagcagg
    10561 ccatgaaatc cggacgcgag ccgctcggta aattgatttt cagaaaagtt acaaacgccc
    10621 tctacacaac gtccctgccg ggagcgcccg acgggcagta tgttgtcatt cagttcaata
    10681 cgtcattcga gaataaaaaa tcgggcattg agaccgttac gccgatggtc gacaaagatg
    10741 ggcggtggag ggtgtccggt tactacatca aataacgacc ggaccctcga gcttagtcgt
    10801 cccaaataaa aatgacgccg cttgtcggga aaatccattc ttcgtcggat attttaacgg
    10861 cgatggattt tcctttcttt cccgttacgg aaggctgcgg atgttttcct gcaaagaaac
    10921 tttcctcgcg gacatttttg atacccgcgt ttgcgtaatc aaattttatc tcaagccctc
    10981 tgacaggata aaccagataa acgggaaaag ttcttttttg tctggcctgc tttgtggata
    11041 tgtccatttc aagtttgaca tcctgattta ttttcttttt gagccggtcg gacccgcacc
    11101 acacctcata accgcgtttt gtgctttttg tttcgatgat gggaacgtct tcattatcga
    11161 tgcgtacccg ttcgacggta aagtcgcgtt tcccggagag gtttccgccg tcgttcaata
    11221 gccagcggta ttcacagcga ggatctttaa ataacgcgtt gagttgcgaa tggctggagg
    11281 cgcagccgat catgaaaata ttgtcgcgca gcgtcttttt gtattggacg tgcatgctga
    11341 catgcagata gtctttggaa agaacggcat
//
LOCUS       DAOOLB010000070        10794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_6912_length_10794_cov_36.067418, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000070 DAOOLB010000000
VERSION     DAOOLB010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10794
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6912_length_10794_cov_36.067418"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="PLF77_08510"
     CDS             <1..228
                     /locus_tag="PLF77_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08932.1"
                     /translation="MMAGKIFYRERIKVGEKEKKPRFKLVAVADVNITFYADHLKKRE
                     LEEIAASVGAELVLIKAPKDGTVKEEEIEVK"
     gene            250..1080
                     /locus_tag="PLF77_08515"
     CDS             250..1080
                     /locus_tag="PLF77_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPC08933.1"
                     /translation="MALFRERDGRRLHVKSRLMGESLVSKKFMEDLKVAPQERLFRDV
                     NIVKIGGQSICDRGVKALPAIVKEVVANKKKHKILLTTGGGTRSRHIYSIGLELGMPT
                     GIIAKFGSSISEQNALLVSTLLAPWGGIKIGHDEITKLYNYFGQDCIPVMHGMPPYDY
                     FALPVAKSRIPIHRTDVGTLVIADLIGARSCIFVKDERGLYTDDPKKNSKAKFIPEIS
                     VQDLLKKNLDDLIIERPCLDILLNSEVIDRIQIINGLEKGNLTKALNGKHVGTIIYKS
                     "
     gene            1473..2036
                     /locus_tag="PLF77_08520"
     CDS             1473..2036
                     /locus_tag="PLF77_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08934.1"
                     /translation="MNAKGIAYIVTKSNMIAAFGEERWSAFMEKLAGKDSYFSTVILT
                     ITPMPVEKLIICFDEMCAEFFNNDKMQYTYFGKAGAKAVLSPTGPYRSFMQMTNDMKQ
                     FIETVLPKVWSSYFDSGVAVAKFENNVAHIKITGLEIKYTYFEYLVMGYFQQAIKMFG
                     KKSSAKRIRSLAAGDKDMYFQYALKDS"
     gene            complement(2134..3960)
                     /locus_tag="PLF77_08525"
     CDS             complement(2134..3960)
                     /locus_tag="PLF77_08525"
                     /inference="COORDINATES: protein motif:HMM:NF013316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HPC08935.1"
                     /translation="MVTSLELLAPAGDAAIGIAAIDHGADAVYIGAPKFSARAAAGVQ
                     LDDIARLIEHAHFYRAKVYVALNTILTDSELAESLDLIRAIDALGADGLIIQDVGLLE
                     LDLPPIPLIASTQMHNSTPEKVRFLEAVGFQRVILARELSLSEIAGIRRQAPKIELET
                     FVHGALCVSYSGQCYMSHAVAGRSGNRGVCAQPCRSHYTLIDGDGNTIMKNKYLLSLK
                     DLNLMSRVPDLIAAGSTCFKIEGRYKGMDYVKNVTAAYRQVIDRFINEHAGYRRSSSG
                     ASELAFTPDVGRTFNRGYSQHFVGGKREKIASLDTQKSIGQYLGKITAAGKDYFQIDR
                     DDLQNGDGLCFFTRQNNLAGSRVNRVEKGKVYPNSMKGLEAGLSLYRNLDTAFAKALK
                     KTSAKRLIAVEMDFSQADTLIRLAAKDEDGNQVEAVKDVLFEPSRNPAGARGQIEKQL
                     TSAGNTPFRISRLTIEPRAPGYLSASVLNGIRRDVLENLARVRRETYVRGDRPFCPND
                     VAYPEKHLDFHANVFNAAARRFYERHGATVTEPAFETLSDFTGKTLMTTRYCIRYQLD
                     LCPRLQQPESSVREPLYLRDAHHTYRLEFDCKHCRMSVILEN"
     gene            complement(3961..4410)
                     /locus_tag="PLF77_08530"
     CDS             complement(3961..4410)
                     /locus_tag="PLF77_08530"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HPC08936.1"
                     /translation="MKIYFAGAIRAGRQDAEIYKAMIEVLMSFGDVLTEHVGNPALSE
                     KGNDGPHDRFIHDRDMAWLESCDCVVAEVTKPSLGVGYELGWATAMKRPVLCLYRRQS
                     KKVLSAMIAGSPGIRTVAYSSMDEARSIVEKFIKKAVQKISTSSGRE"
     gene            complement(4688..5173)
                     /locus_tag="PLF77_08535"
     CDS             complement(4688..5173)
                     /locus_tag="PLF77_08535"
                     /inference="COORDINATES: protein motif:HMM:NF025559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6125 family protein"
                     /protein_id="HPC08937.1"
                     /translation="MKLTETQMIEIAQHAFTRMDGAWFLALAKKFGVQTAWEMDVEAW
                     KHFAYVFGKNLRKNYLPNPVWPESIFDALDIFSRVLKIEGRKVCIEDGKIIIRITDCE
                     TQKAIAKAGVADCGIVTVQTYQGLIGGLFDNKIFVDVAHTKNLNRGDDCCEVVVTRKS
                     P"
     gene            6166..6480
                     /locus_tag="PLF77_08540"
     CDS             6166..6480
                     /locus_tag="PLF77_08540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HPC08938.1"
                     /translation="MSIKYDGKRKVKPTHPGEILKEDFMPDYNLNVATLAKALGVSRQ
                     TVNELLRESRALTPIMALRLGRLFGNTAEFWLNAQRAHDLWEAQQTHQAEIKKIHPLK
                     AA"
     gene            6638..7495
                     /locus_tag="PLF77_08545"
     CDS             6638..7495
                     /locus_tag="PLF77_08545"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPC08939.1"
                     /translation="MNKSKDKLSALIIYLSPHGTTKKAIDTLAVSLNENSVSSDIYNL
                     SEFDNNDIMAKIADYQLIVLGSPTYFHHAPTVFTDFINNMPDASNDQSVVLLSSFGGV
                     SSGVVLYDLAKILHRKKYNLIGGIRVLTEHCLTFQEEKPFYSGHPDKKALNAIKTFGK
                     EISVRLKNKICTRYSPSDFNDKPFILNFMDDHIVKLRNFVWAMPGITVNQSLCNRCAV
                     CVKNCPTHNIKLGKATMHGGNCIYCYCCVRNCPVHATIAFLKPASITVKLMAEIFRKF
                     EDQETQQIV"
     gene            complement(7736..8773)
                     /locus_tag="PLF77_08550"
     CDS             complement(7736..8773)
                     /locus_tag="PLF77_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HPC08940.1"
                     /translation="MRISLKSKPVIAPYRSTHNLRVIIQWAEEHGIDTASILSSEGLE
                     KADFDNPDRLITFEQQFEISQKLFDLISCPALGLRIGERINIGQLGKWIMAINSCATT
                     LDAFKMAFELADLLSFPFQFILDVQDDIAKFSFIDLIDLGRSSRFRYEMHMVGGYNIC
                     CEIMRERVQLIEIRFAYSKPEYAEEYKPIFNCPILFDAEETQIRFDSSYLTRPLPCAN
                     TLTKKIYEKDCRQTLEQLIMLTTTTELAKSALQFKDKELLTRSQLARCLNMSDQTLKR
                     RLAAEGVSYKSLVKDIRQKKACDLLQSTELTMEEIANKVGYRDVANFYRAFKSWTGLT
                     PRSCRGKNEKR"
     gene            8996..10513
                     /locus_tag="PLF77_08555"
     CDS             8996..10513
                     /locus_tag="PLF77_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08941.1"
                     /translation="MRKGLRLFPMMTMLILFVLTVFSVEIFAWPVIPPPNFTNEPPRN
                     PFIADSPWPMSHRNPYGQASSPYPGPKSINLTTRSSFHVGMPGLITLAISGKYPDGKR
                     VIWGSSSTDVFKADESLNYIKTMKKENLTISSIFTPGEMLSGAYTLVDKDNTFYMPRF
                     TKIFAFGDAIAGNRYSDIIIQGVYEIPANLLVDPAEKIVGLSMTWDGMLTLATSHGLI
                     GVVSRFFDEAYYYRFPADEQISNSIACDEHGGIYVVTDKNMYRVQWTGSELTIDPDKG
                     GWSAGYETGEASGVRLGAGSGSTPTLMGTGNQDKFVVITDGQNLMHLVLFWRDEIPAD
                     WQQIPGTKSRRIAAQVPVTFGNPGATETLSEQSVCVRGYGAVVVNNQLKASLDNQVIS
                     VLMSGFAPIAPYGVEKFVWDPWTRTLKTAWVNKSVSIPNGIPTMSQTTNLFYGVGKRF
                     GFWTFEALDWTTGKSVFSYPYGITPANNSAYAATEIGLNGSLLTGTLTGMVLMKP"
BASE COUNT         2949 a         2510 c         2538 g         2797 t
ORIGIN      
        1 atgatggcag ggaaaatatt ttatcgcgaa agaatcaagg ttggcgaaaa agagaagaag
       61 ccacgtttca aactggtggc ggttgccgat gtgaatatca ctttttacgc cgaccacctg
      121 aagaaaagag agctcgagga aattgccgct tcggtgggcg cggaacttgt tttaatcaag
      181 gcgccgaaag atggaacagt caaggaagaa gaaattgaag tcaagtaaac attcttgagg
      241 attgaattta tggcactgtt cagagaaaga gacggacgca ggctgcatgt gaaaagcagg
      301 cttatgggcg agagcctggt gagcaaaaaa tttatggaag acctgaaggt ggctcctcag
      361 gaaaggcttt tccgggatgt gaatatcgtc aagatcggcg ggcaatccat ttgcgatcgc
      421 ggggtgaagg ccttgccggc catcgtcaag gaagttgtcg ccaacaagaa gaaacacaag
      481 attctgctga cgacaggcgg cggcacgcgc agccgccaca tttattccat cggtttggag
      541 ttggggatgc ccaccggcat catcgccaag ttcggcagtt ccatttccga acagaacgcc
      601 ctgctggttt ccacactgct ggctccctgg ggcggcatca agatcggcca tgatgaaatt
      661 acaaaactat acaattattt cggccaggac tgcattcccg tcatgcacgg catgccgcct
      721 tacgattact ttgccctgcc cgtggcaaaa tcccgcattc ccattcaccg caccgatgtg
      781 ggcacgctgg tgattgccga tttaatcggc gcccgcagtt gcatttttgt caaggacgag
      841 cggggactct acacggacga tccgaagaaa aacagcaagg cgaaatttat tcccgaaatc
      901 agcgtccagg atcttctgaa aaaaaatctt gatgatctga ttatcgaaag accgtgcctg
      961 gacatcctgc tcaacagtga agtcatcgac aggattcaga ttatcaatgg tctggaaaag
     1021 ggcaacctga ccaaggccct taacggcaaa cacgtcggca caatcattta caagtcatag
     1081 aagtcaggcc gcatcatcac aaaaaagaag gcttcggtat tacgaagcct tctttttttg
     1141 caatcagatt tccccctgat tatccgccat aaaatctttt tgttgctgcc gatgagacaa
     1201 acagtaaagt cggccattac tggaacagca attatgtaaa aatgacacat atccctcttt
     1261 agagggattg attaattttc cggagcaatg tagattgatc gccgtgatac gttttgccgg
     1321 acatccggca gtgatgcgaa tcgcttccga tcgctcattt taaagattca taattttcta
     1381 tccggccatt ttatgttaga ttgcaccatg ccgcacataa cagacgttgg aaacaccttg
     1441 atgacagaac aatccggggg aggatttcag acatgaacgc taaaggcatt gcttacattg
     1501 taacaaaatc aaatatgatt gcagcatttg gcgaggaaag atggtcggcc tttatggaaa
     1561 aactcgccgg gaaggacagt tacttcagca cggtcatcct caccattact cccatgccgg
     1621 tggaaaaatt aatcatttgt tttgatgaaa tgtgtgccga atttttcaac aacgacaaaa
     1681 tgcagtatac atatttcggc aaggcgggcg ccaaagcggt tctgtcgccg acgggaccct
     1741 acaggtcgtt tatgcagatg acaaatgaca tgaaacaatt tatcgagacg gtgctgccca
     1801 aggtctggtc ttcttatttt gacagcggcg tcgccgttgc aaagtttgaa aacaatgttg
     1861 ctcatatcaa gattaccggt cttgaaatca aatatacgta ttttgaatac ctcgtgatgg
     1921 gttacttcca gcaggccata aaaatgttcg ggaagaaatc gtctgcaaaa aggatcagga
     1981 gccttgccgc aggggataaa gatatgtatt ttcagtacgc gcttaaagat tcctgaccat
     2041 ccgcgccggt gcagtatttt taatactctg taattgatcc ccgcttaacg gatagaaata
     2101 cccgcgcgcc ttaatccggt ttccgcgctt ccgtcagttt tccagaatta ccgacatccg
     2161 gcagtgcttg cagtcgaatt caagacgata ggtatgatga gcatcccgaa gatacaacgg
     2221 ttctctgacg gaggactccg gttgctgcag tcggggacac agatccaact gataccggat
     2281 gcagtagcgc gtggtcatga gggtttttcc ggtaaaatcg gaaagcgttt caaaggccgg
     2341 ttcggtaacg gttgcgccgt gccgttcata gaaccgtctg gccgccgcat tgaaaacatt
     2401 ggcgtgaaaa tcgaggtgtt tttccggata agcaacgtca ttgggacaaa acggccggtc
     2461 cccgcgaaca taggtttcac gtctcacgcg cgccagattt tccagaacat cgcgccggat
     2521 gccgttcaag acgcttgcgg aaagatagcc cggcgcgcgg ggttcaatgg tcaggcgcga
     2581 tatcctaaac ggcgtgttgc cggcgctggt cagttgtttt tcgatttggc cccttgctcc
     2641 ggccggattg cgcgacggtt caaacaaaac atcctttacg gcttccacct gattgccgtc
     2701 ttcatctttt gccgccagcc ggatcaacgt atcggcctgg ctgaaatcca tctccaccgc
     2761 aatcaatcgt ttggccgatg ttttctttaa cgctttggca aacgcggtgt cgagattgcg
     2821 ataaagcgac agtccggcct ccaggccttt catgctgttg ggataaacct tccccttctc
     2881 tacccggttc actctggagc cagccagatt gttttgcctg gtgaaaaagc aaagtccgtc
     2941 cccgttttgc agatcatcac ggtcgatttg aaaataatct tttcccgccg cggtgatttt
     3001 ccccagatac tggccgatgg atttttgcgt atcgagcgaa gcgatctttt ctcttttccc
     3061 gccgacaaag tgctggctgt aaccgcggtt gaacgttctg ccgacatcgg gcgtgaatgc
     3121 aagctcgctt gcgccggaac tgctacgccg gtaaccggcg tgctcgttga tgaatcgatc
     3181 gatcacctgc cggtaagcgg ccgtgacgtt tttgacatag tccatcccct tgtatcgccc
     3241 ctcgatttta aaacaggtcg agcctgcggc aatcaaatcc ggcacccggc tcatcaggtt
     3301 caaatccttg agcgaaagga gatatttatt tttcattatg gtgttgccgt caccgtcgat
     3361 cagcgtgtaa tgggaccggc agggctgcgc gcaaacgccg cgattgccgc tgcgccccgc
     3421 aacggcatga ctcatataac attgtccgct gtaggaaacg cataacgcgc catgcacaaa
     3481 cgtttccagt tctatcttcg gggcctgacg gcgaatcccg gcaatctcgg aaagagaaag
     3541 ttcccgggcc agaataaccc gctgaaatcc cacggcctcc agaaaccgga ccttctccgg
     3601 cgtactgttg tgcatctgag tgctggcaat cagcggaatg ggcggcaagt ccagctccag
     3661 caaaccgaca tcctgaataa tcagcccgtc ggcgcccagc gcatcgatgg ccctgatcag
     3721 atccagcgac tcggccagtt ccgaatcggt aagaatggta ttgagcgcca cataaacttt
     3781 cgcccgatag aagtgggcgt gttcaatcag acgggcgata tcgtcgagct gcacaccggc
     3841 tgcggcccgg gcgctgaatt tcggcgcgcc gatatacacg gcatccgcgc catgatcgat
     3901 ggctgctatt ccgatagcgg catcgcctgc cggcgccagc aattccagtg atgtgaccat
     3961 ctattctctc ccggaagaag ttgatatttt ttgaacggcc tttttgataa atttctccac
     4021 aatacttctc gcttcgtcca tggaggaata ggcaaccgtc cggatgccgg gactgcctgc
     4081 aatcatggcg gacaaaactt tcttcgattg gcgtcgatac aggcacaaaa caggcctttt
     4141 cattgccgtg gcccatccca gttcatagcc cacacccaga gaaggtttgg tcacctcggc
     4201 caccacgcaa tcgcaggatt ccagccaggc catgtcgcgg tcatgaataa accggtcatg
     4261 cggtccgtca ttgccttttt ccgacagagc gggattgccg acgtgttcgg tcaggacgtc
     4321 gccaaatgac attaaaacct caatcattgc tttgtagatt tcggcatcct gccttccggc
     4381 gcgaatcgct ccggcaaaat aaattttcat aaactcccta cgcccaggcg atgaaacact
     4441 tttgctattg ataccactga tggtcggatg cattcaacac atttaaggag actccaatca
     4501 ccaaagtgaa atgcatcgga attggttagt tgaactatcc aatagctatg ctattggaga
     4561 ctatccccga agcgaagtgg aggggacact cttcaccctt cacgtttcac gattcacaat
     4621 ttgtttaacg ttgaacattg aactattttc tattcacccg tttatcggtg gatatttata
     4681 tggacattta cggcgatttc cgggtgacaa caacttcgca gcaatcatca ccgcgattta
     4741 aatttttggt gtgagcaaca tccacgaaaa ttttattatc aaacagaccg ccgatgagcc
     4801 cctgataggt ctggacggtg acaataccgc aatcggcaac gccggccttg gcaatggctt
     4861 tctgtgtttc gcaatccgta atgcgaatga taattttacc atcttcgatg caaactttcc
     4921 gtccctcgat ctttaaaacc ctgctgaaaa tatccaaagc atcaaaaata ctttccggcc
     4981 agacggggtt ggggagatag ttcttccgga ggttcttgcc gaaaacataa gcaaagtgtt
     5041 tccaggcttc cacatccatt tcccaagcgg tttgaacacc gaacttcttc gccagcgcca
     5101 aaaaccaggc cccatccatg cgcgtaaacg cgtgctgtgc aatttcaatc atctgtgttt
     5161 cagttaattt catttctaaa aacttccttt catctattgg gagtgaagta taaggaacga
     5221 tttcttgtga gtcaagaagg ttgtcgctac aacacgctga cgataagaag cctattcact
     5281 atttaatgag acttgctgaa aaaaagcttt gcgatgtaat caaacccaag ttttagatgc
     5341 gtagcgtcct gaaacttgta ggttgaatta tcacctatgg tgatttgaag cgcaagtcgt
     5401 agtgacaccc gttgagcgcg agaaaaacga cgcgcaaaac gttggaggaa ctatccaaaa
     5461 gccttcggct attggaaact atccccgaag tgaaacggag agggggaaaa gcggaaaagg
     5521 gggacgccct taaaattgta aaaagggcaa aggggtcaag tctttgcttg gctcatgttg
     5581 gaaagaaaaa gtggattccc accagacccc tgcgggaatg acaccttaaa taaaaagaca
     5641 tgcctctgaa aagaagcatg tctttttggg tttttgaatt ggttaaaatt tacgcatctt
     5701 ccacttttac cgcgcagacc ttgtattccg gaatttttcc ggtcgggtcc agagccgggt
     5761 tagtcagtag gttgacggat gcatccgagt tggacaatgg cagaaaagga catcattaga
     5821 atcaaaactt tgcccttgac aaatgtcacg agtcacgtta cacaatacac atgataaaaa
     5881 catttgccga caaacatacg cgtgatttgt ttattaccgg taaatcaaag aagtttcagc
     5941 ctgatattct taaaagagct atcaggcgtc ttgaatatat agatttggca acgtgcattg
     6001 aagatttaaa agtgccgccg agcaatcatc tgcacatgct gaaggataac agagaaggtc
     6061 aatatgcaat atccgtgaat gaccagcgga gaatatgttt ccggtttatt aacggtgatg
     6121 catacgatgt tgaaataacc gattatcatt aaaagaggta agaatatgag cataaaatat
     6181 gatgggaaaa gaaaagtaaa gccgacacat ccgggtgaaa ttcttaagga agattttatg
     6241 cctgattata atcttaatgt tgccactctg gccaaagcac tcggtgtatc gcgtcagacg
     6301 gttaatgaat tattaagaga gagccgcgct ttgacaccga tcatggcgtt acgtcttggc
     6361 cgtttgttcg gcaatacggc tgagttttgg ttaaacgctc agcgcgctca tgatctatgg
     6421 gaggcgcagc agacgcatca agctgaaata aagaaaatcc atcctctgaa agcggcttaa
     6481 ttgtggggca cattccctga acgcgccgta tatggattat tcccaattct aatttattga
     6541 aatgaaaata aatggctagc gggacgccct taaaattgta agttactgtc atattcgggg
     6601 aacataaaac taaaaacaca atgacaatgg tcacaaaatg aataaatcaa aagataaatt
     6661 atccgcctta atcatttatc tttctcctca tggaacaacg aaaaaagcta ttgatacatt
     6721 ggcagtgtcg ctaaacgaaa acagcgtgag ctctgatata tataatctga gtgaatttga
     6781 taataacgat attatggcaa aaatagcgga ctaccagttg atcgttttgg gctcgccgac
     6841 atattttcat catgcaccta ccgtgtttac tgattttatc aataacatgc ccgatgcatc
     6901 gaatgatcag tcagtcgttt tgctttcctc ttttggcggg gtatccagtg gtgttgtttt
     6961 atatgatctt gctaaaattc tgcacaggaa gaaatataat ttgatcggcg gcatcagagt
     7021 cttaacagaa cactgcctta cctttcagga agaaaaacct ttttattcag ggcatccgga
     7081 taagaaagcc ttgaacgcta tcaagacgtt cgggaaagaa atatctgtcc gattgaaaaa
     7141 taaaatctgt acaagataca gcccttctga tttcaatgat aagcctttta ttctaaactt
     7201 tatggatgac cacattgtta agctgagaaa ttttgtatgg gccatgccgg gcataacagt
     7261 aaatcaatca ttatgtaacc gctgtgctgt gtgcgttaaa aattgcccca cgcataacat
     7321 aaagctcggt aaagccacca tgcacggggg aaattgcatt tattgttact gttgtgtcag
     7381 gaactgcccg gttcatgcga caatcgcatt tctcaagcct gcgagtataa cggtcaaact
     7441 gatggcagaa atattccgca aattcgaaga tcaagaaaca cagcagatag tttgatcgtt
     7501 taggagtaag aggaacgccg ttaaaattcc aagttactgg ttaaaaagca acaggggcaa
     7561 aggggtcaag tctgctcttg actcatatta gcggtttttg cgctggttca atcgtcgtcc
     7621 ttcgattgaa ccagaatatt tcggagcaat cagcaacctg tccggcgcat ccggcattct
     7681 ccggagcacc tgccggatga ttgcttcggc aatgaaaagt tcatccattg ggtgattacc
     7741 gcttttcatt tttcccacgg caactacgcg gtgtcaggcc tgtccacgat ttgaaggcgc
     7801 gataaaaatt ggccacatca cgataaccta ccttatttgc aatttcttcc atggtaagtt
     7861 cggttgattg cagaagatca caagcctttt tctgacggat gtctttcacg agtgatttat
     7921 aactcacacc ctcggccgct aaccggcgct ttagcgtttg atcgctcatg ttcaaacacc
     7981 gggcaagctg tgacctcgtg agcagttctt tgtctttaaa ctgaagcgcc gatttagcca
     8041 actccgttgt tgttgtaagc atgataagct gctcaagcgt ttgacggcaa tctttttcat
     8101 aaatcttttt agtcagcgta ttggcgcatg gcaatggtct ggtcaggtaa ctgctgtcga
     8161 accgaatttg agtttcttcg gcatcaaaga gaatcggaca attaaatatt ggtttatatt
     8221 cctctgcata ttccggcttg gaataggcaa aacggatttc tataagttgc actcgctccc
     8281 gcataatttc acagcagata ttatagccgc ctaccatatg catttcgtaa cggaagcggc
     8341 tgctgcgacc cagatcgatg agatcaataa aactgaattt ggcaatatcg tcctgaacat
     8401 caaggataaa ttggaaggga aatgataaga gatcggcaag ctcaaaagcc attttaaagg
     8461 cgtcaagggt tgtggcacag ctattgatag ccattatcca tttccccaac tggccgatat
     8521 taatgcgttc tccaatgcgc agtcctaaag ccgggcagga aataaggtcg aataactttt
     8581 gcgagatctc aaattgctgt tcaaatgtta tcaatctatc cgggttgtca aaatccgctt
     8641 tctccagtcc ttcactggag agaatcgacg ccgtatctat tccatgctct tctgcccatt
     8701 ggatgataac gcgcaagtta tgcgtagatc gatagggcgc gattacaggc tttgatttca
     8761 gagaaattct catgattcaa tcagtattat tcttcgccat taagttgatc caaaatgaca
     8821 atattttgag cttctccggc aattgtaaat gatatccgta tcaagtagca taaaacgaaa
     8881 ttaaccaagt ggtcaataac acaagcgtag atgaaactca atgtcgttgt caatgtaaag
     8941 taatatcaaa cgtgccgtac actattaaaa gaaatcgctg aactggaggc tatttatgag
     9001 aaaaggatta agactgtttc cgatgatgac aatgttgatt ttgtttgttc tgacagtgtt
     9061 ttccgttgag atttttgcct ggccggtcat accgccgcca aactttacga atgaaccgcc
     9121 ccgaaatccg tttatcgctg attctccctg gccgatgagt catcgaaatc cttacggtca
     9181 ggcatcctct ccgtatccgg gacctaaatc aatcaattta acaacaagat catcttttca
     9241 tgtcgggatg cccggtttga ttacgcttgc catctctgga aaatatccgg atggtaaacg
     9301 cgtgatttgg ggcagttcat caacggatgt gttcaaggcg gatgaatcgc taaattacat
     9361 caaaactatg aaaaaagaaa atttgaccat ttcatctata tttacacccg gtgaaatgct
     9421 ttccggcgct tatactctgg tggacaagga taatacattc tatatgcccc ggtttacaaa
     9481 aatatttgcg tttggcgatg cgattgccgg taatcgctat tcggatatca ttattcaggg
     9541 cgtgtatgaa attcccgcaa acctgcttgt tgatccggcg gaaaaaatcg tgggactaag
     9601 catgacctgg gacggcatgc tgactcttgc caccagtcat ggattgatag gggttgtttc
     9661 ccgttttttt gacgaagcat attactatcg cttccccgct gacgaacaga tttccaattc
     9721 catagcctgt gatgagcatg gaggcattta tgtcgttacc gataaaaata tgtaccgagt
     9781 gcagtggact ggcagtgaac tcacgataga tcctgacaag ggaggatgga gcgccggtta
     9841 tgaaacggga gaagcttcgg gcgtgcggct cggagcaggt tcaggctcga caccgacgct
     9901 aatgggaacc ggtaatcagg ataaatttgt ggtgattaca gatggtcaga atctcatgca
     9961 ccttgttctt ttctggcgcg acgagattcc cgccgactgg cagcagatcc ccggaacaaa
    10021 aagccggcgc attgccgcac aggtgccggt gactttcggt aaccccggcg cgacagaaac
    10081 attgtctgaa cagtctgtat gcgtcagggg ttatggagca gtggttgtca ataaccagtt
    10141 gaaagcgtca ctggacaatc aagtcatcag cgttctgatg tccggatttg cgccaatcgc
    10201 tccttatggt gttgaaaaat tcgtatggga tccatggaca cgaacattaa agacagcctg
    10261 ggtaaacaaa tcggtaagca tccccaatgg aattcccacc atgagtcaga cgaccaactt
    10321 gttttatggc gtcggaaaaa gattcgggtt ttggacattt gaagccttgg attggacgac
    10381 aggaaaatct gtcttcagct atccatacgg cattacaccg gccaataaca gcgcctatgc
    10441 cgccacggaa atcgggctta atggcagcct cttaaccgga actttaaccg gcatggtact
    10501 gatgaaacct tagcctgtct gccggcattg ggcatttaag ggacataaca cttaattaat
    10561 aaaaaaaccg gatttaccct gacgggcacc atggcccgcc agcgcgggaa tgacacttta
    10621 gagaaagaaa gctatacccc ttttcagagg catagctttc tttgtttcta ttaatatttt
    10681 aaaagctacg cggcttccac ttttaccgcg caaaccttgt attccggaat tttgccgatc
    10741 gggtccagag ccggattggt caacaggttg acggatgcgt ccgcgaaatg gaag
//
LOCUS       DAOOLB010000071        10482 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_7209_length_10482_cov_33.170423, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000071 DAOOLB010000000
VERSION     DAOOLB010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10482
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7209_length_10482_cov_33.170423"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1204
                     /locus_tag="PLF77_08560"
     CDS             152..1204
                     /locus_tag="PLF77_08560"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HPC08942.1"
                     /translation="MRKVTETPSRMVEKDGTIHFGTFRTPFRDVNILDVPLYSSSFGI
                     PAFWKKFRLKEWQHYGIITPTHYFGMVVFDAKFMGVSFFYAYDRMENKRFEHGAQAPG
                     GASLVAAQMYDGICEFKQKGYLLRFENQLKDGFHKIIIDIAEKKNRPAVQGEINVLED
                     LSKIEPLVQVSPVTPLRPFYTHKVAAPAEGEIMLGSKTVALNHGQDVALIDEQKTYYP
                     YVSFWKWSTAAGYNEYGELLAFNLCQNMIGDDEDHNENCMWVDGKIYCLKAARFKFEE
                     DNVLHPWEVRTNDGYMDLLFTPSGERAEKISIGLIGSDFHQPFGLYNGRFKDHNNRIY
                     PITDFFGLAEHHVTRY"
     gene            complement(1191..2315)
                     /locus_tag="PLF77_08565"
     CDS             complement(1191..2315)
                     /locus_tag="PLF77_08565"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPC08943.1"
                     /translation="MPNNSTTTPYVRLGFLDALRFFAIIYVIISHLILIPQPNLAIPE
                     WIAPFLINGGDAGVSLFFVLSGFSLSYTMDKRAKEKRLLHNFYIRRFFRIAPLFYLLM
                     AIYWIRDAAVFGVFHSLSEVLINASLLFNLVPSCITGFVWASWTVGVIALLYLIFPTI
                     HKHIRNLHGALALFVISVLTAWGWSYFIKNYGVAVGYLNADDIGFVWGFGFLQHLPVF
                     VCGIVIYRLFFDYLIKTTRESRQHYGMISILAFLLFYGILLTESMQNLLWGRTILHGI
                     CFSLLVLGLALSPFRFLVNAKTAYLGKISYSMYLFHPLIIFTAIPVYRLLYASMSSSL
                     PGYLSSLLVTLILLMIISLISYRWIERTGMALGEKLISNE"
     gene            complement(2366..3697)
                     /gene="purD"
                     /locus_tag="PLF77_08570"
     CDS             complement(2366..3697)
                     /gene="purD"
                     /locus_tag="PLF77_08570"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HPC08944.1"
                     /translation="MPNILLLGNGAREHALAEAITRSGQKPSLFSFMKSNNPGIASLS
                     EKTLIGSYSDLDAITKFATENKIEFAVIGPEDPLNNGVADALAERNIPAVGPTKSLAR
                     LETSKSFTRNLVSKYSIPGNPQFKVFKTLDGVEDFLNQLEGIVLKPDGLTGGKGVQVQ
                     GDHFDSKKEALKLCAQILAGGASLVIEEKFDGEEFSLQCLCDGKTVVATPLVQDHKRR
                     FDGDKGPNTGGMGSYSLADHSMPFLRPQDLHDGLEITRQVAEALLKETGSPYKGIMYG
                     GFIATKDGVKLLEYNARFGDPEAMNILPLLKTDFVDVCRHIIAGTLNQIKIEFDKKAT
                     VCKYVVPKGYGLPADHPDAASSRAKIEVGNVGPARLYYSSVDQKDDGLYLSSSRAIGI
                     VGIADNLADARKIAEEGVEAVKGPVAYREDIGTDALIQKRIDHMKKIRKDA"
     gene            4006..5274
                     /locus_tag="PLF77_08575"
     CDS             4006..5274
                     /locus_tag="PLF77_08575"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPC08945.1"
                     /translation="MGWNSDIVVLILLFMLSGFFSSSEAALLSLRDLHLHKMQQNNYP
                     FFNYVVKLLSNQRRLLITIVTSNEVVNVSISILAASFFIGLLGSSGQWVSIVFTTVVL
                     LFVGEAIPKTFGVTYPMQISSLVSPILMAVSRIESPVVSALEKVSGLFLRCYGVNKSE
                     ESEVIMEHEFKTLVDVGSLEGVVNEAQKELINKIFELGDRPVTDIMVPRVDMFCLPLD
                     MPVGDMLQSVAAARQERVPVYREDRDDIVGILFARDLLNNVWRGRLQTGIFNLLARPF
                     FVPEQKTINGLLHDFQKNFIQIAIVVDEYGGVSGMITLEHILEHLFEETEGDEEMVCD
                     GCEKIDEQSIIVPGSMSVEQCNKLLEAELPEDEFDTVGGLVLHLFGKIPKKGEEAHLG
                     GYVFCVHDVGKTRILKVRITKKTVEEPEES"
     gene            5271..6551
                     /locus_tag="PLF77_08580"
     CDS             5271..6551
                     /locus_tag="PLF77_08580"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPC08946.1"
                     /translation="MSLYLIVAAILFCLMLEMLYSGGEVALFASDINKLKNRAHQGST
                     SAHQAVKLKEKPEWFISTALVGTNLAIIIASTLITGLLISFYGPARGEKIAFLIMIPT
                     LFLMIIARSIFLYYAEAMAMRVAWFIRFSSILFYPIAFVIAAVSRSTVHFSSDTRSGQ
                     SSHITKEGLKYILGEKTKGGDILTREKEMVSRVFDFSELTAMKIMVPVSALTALSASM
                     KISEAVGVVADKKYMRIPVYEDTVYNIVGILRYFDLFEMLLKDKEKGEGVGEATVASC
                     MRKDVFYVPETKKVSELLAAMQKRDEQMAVVVDEYGGATGVVTIEDIGEEIVGTIDEY
                     VSGEKLYRKIAEGRYLVSGRLEIGTLRQLVKAKFPEGNYETVGGFLMYQMGKIPRIKE
                     YYQYGDVTFIIENADQKSIKEVTVIAPFIQEGNK"
     gene            6669..7868
                     /locus_tag="PLF77_08585"
     CDS             6669..7868
                     /locus_tag="PLF77_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08947.1"
                     /translation="MKSDLTRQDWEEYLIRLYFGTKNDLIKSCINRAYRDFNRTLHGM
                     DDHKNKQDLFDRAVKYLLSIFIDIKKESLNDHEEFDKWHKEKCLHLKNLYKENDFINF
                     SIGQAQKWINMTFKYIFCVGEKRLPGYSGLYEFCHVPIDNIILDKIKKRGAPVPSKPW
                     SRLGDYNEYISLQKWFREQYKLPLDGEFILWMEEPHDNVIMPVQNNIVLDKTRVKEAK
                     DETIGPYQLNRIILCSADASPASQKKDKYKAEYFFPSAKWVGAVRNVASDLGCKFVIL
                     TTKHGLVNPSDDIDHYDLEIKGNENIINEKWQDTTSRLIRRGQYDILVFYAGGCPRDE
                     YLRILSPILKNLEIDLLTFGKPNMGDAGKIKDIVESLGEEASIDTLKKKLKFPDRLLF
                     FPHNNKA"
     gene            8042..8638
                     /locus_tag="PLF77_08590"
     CDS             8042..8638
                     /locus_tag="PLF77_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HPC08948.1"
                     /translation="MLSKNMAELQELSRKTCFTLEDVAQSLGMQTASARVLCSRYVRQ
                     GVLIRLKKGFYTTAWKWENLNRQDFFKIANILQVPSYISLMSALAYYEVTTQAQNNYQ
                     ENICLKRSIVYNVREAVFNYVKVQARYYGDFVKTDGIFIATKEKAFVDAAYLYSFGKY
                     KFDVDSLDMKKLDIKKLRSIIKIYPQKTKETVKRLCWI"
     gene            8626..9288
                     /locus_tag="PLF77_08595"
     CDS             8626..9288
                     /locus_tag="PLF77_08595"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPC08949.1"
                     /translation="MLDLIQHEQFELEVLNRLQSGRFLANLIFGGGTMLRLCHGLNRY
                     SVDLDFWVVREMDWAKYYSRMEKYLGQFYKIADSANKHFTILFELKSPQFPRSLKIEI
                     RKTVKNVKTETSIAYSPHAPVQVMLKTVALKDMMTSKIEAFLDRREIRDAYDIEFLVK
                     RGIVLNADHEVITQMLTKLGKLGKKDFSVKLGSLLDVSERKYYVENGFRILEANLRDK
                     LR"
     gene            9338..10090
                     /locus_tag="PLF77_08600"
     CDS             9338..10090
                     /locus_tag="PLF77_08600"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HPC08950.1"
                     /translation="MFDKVNETKFCALILAAGKGTRMKSDKAKVLHELNGKPLLYYSI
                     EAARAVGVEKIVAVIGHQAERVRETFAANGCIFVEQNPQLGTGHAVLQAKDVLTDYKG
                     FTVILCGDVPLLKPATIKSLVDNHVAEKAVVSVLTTIPPHPHAYGRIVKDSKGNVLKI
                     VEHRDATEEEKKIGEINTGIYCVDTEFLFHALGKVTNNNRQQEYYLTDIVEIAVCEGQ
                     KVKSFIADDYVEVMGINTLEELTRAEKYLRKG"
     gene            10305..>10482
                     /locus_tag="PLF77_08605"
     CDS             10305..>10482
                     /locus_tag="PLF77_08605"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HPC08951.1"
                     /translation="MKNINNIIEAVAKKGALLAPLAGISDLPFRLIARSQGCSLAYTE
                     MISSNGLVRNTRKTY"
BASE COUNT         3028 a         2149 c         2538 g         2767 t
ORIGIN      
        1 cgcccagggc gataaaatca tctccgtgaa aattgaagga gatgttgcgc cgctgatgga
       61 aaaaatcaaa ccggtattgg ataaatggaa tgcggtcctt gatgtaaatt tcccgaagct
      121 tgcgaagggt taatacaata aaaaagacgc gatgagaaaa gtaacagaaa ctccctccag
      181 aatggtggaa aaagacggaa cgattcattt tgggacgttt cgcacgcctt ttcgcgatgt
      241 gaatattctg gatgtgccgc tttattcgtc ttcattcggc attcccgcat tctggaaaaa
      301 attccgtctc aaggaatggc agcattacgg cattatcacc cccacccatt acttcggcat
      361 ggtcgttttt gatgccaaat tcatgggcgt ctcctttttt tacgcttatg acagaatgga
      421 aaacaaacgc tttgagcatg gtgcgcaggc accgggcgga gcatcgcttg ttgccgcgca
      481 gatgtatgat ggcatctgtg agtttaaaca aaaaggttat ctcctccgtt ttgagaatca
      541 actcaaggat ggttttcaca aaatcatcat cgatatcgca gagaaaaaaa acaggcctgc
      601 cgtccagggg gaaatcaacg ttcttgagga tctcagtaaa atcgagccgc ttgttcaggt
      661 ttctccggtg acacctttaa gaccttttta tacccacaag gtggctgcgc cggcggaagg
      721 tgaaataatg ctgggctcta agacggttgc tcttaatcac ggacaggacg tggcattgat
      781 cgatgaacag aaaacctatt atccctacgt cagtttctgg aaatggtcga cggccgccgg
      841 ctataacgag tacggggaac ttttagcctt caatctctgc cagaacatga tcggtgatga
      901 tgaagatcat aatgaaaact gcatgtgggt ggacggtaaa atctactgtc tgaaagcggc
      961 gcgttttaaa tttgaagaag acaacgtgct gcatccatgg gaagtcagaa caaatgacgg
     1021 atacatggat ctgttattta caccctccgg tgaaagagct gaaaaaatca gcatcgggct
     1081 gattggatcc gattttcacc agccgttcgg cctctataac ggacgattca aagaccacaa
     1141 caatagaata tatccgatta ccgatttttt cggattggcc gagcatcacg tcactcgtta
     1201 ctgatcaatt tttccccaag agccattccc gtccgttcaa tccatctgta agatatcagc
     1261 gagataatca taagcaaaat aagcgtcacc agtagggatg agagatagcc cggcagggat
     1321 gatgacatcg atgcgtaaag cagccgataa acgggaatgg cggtgaaaat gatgagcgga
     1381 tgaaaaagat acatcgaata actgatcttg cccagataag cggttttggc gttgaccaga
     1441 aagcggaaag ggctcaatgc cagccccagc acaagcaatg aaaaacagat gccgtgcaga
     1501 atcgtcctgc cccagagtaa attctgcata ctttcggtaa gcaggatgcc gtaaaacagg
     1561 agaaaagcca ggatggatat cattccatag tgttgtcggg attcccgcgt cgttttgatc
     1621 aggtaatcaa agaacagccg ataaattacg atgccgcaga cgaataccgg caagtgctgg
     1681 agaaaaccaa agccccagac gaagccgatg tcgtccgcat tcagatatcc gaccgccacg
     1741 ccgtagtttt ttataaaata cgaccatccc caagcagtca gaacggatat gacaaacaaa
     1801 gccagcgctc catggagatt gcggatgtgt ttgtggattg tcggaaaaat cagatacagc
     1861 agggcaatga ccccgacggt ccaactggcc cacacaaatc cggttatgca ggatggcacg
     1921 agattaaata aaagagaggc gttgataagc acttcagata aagaatgaaa tacgccaaaa
     1981 acagcggcat cgcgaatcca atagatcgcc attaaaagat aaaaaagagg cgcgattctg
     2041 aagaaacgcc ggatgtaaaa attgtgcaga agccttttct ctttcgcgcg tttgtccatg
     2101 gtataggaga gggagaaccc gctcaagaca aagaagagac tgacgccggc gtcgccgccg
     2161 ttgattaaaa acggggcaat ccattcgggg attgcaagat taggttgagg aatcagaatc
     2221 aggtggctga tgatcacata gatgattgca aaaaagcgca gcgcgtccag aaaaccgagg
     2281 cgtacatagg gcgtggtcgt agaattgttt ggcattaaac gctccgtaaa attggagtat
     2341 tccggcaaaa gaaataaaaa actctttaag cgtcttttcg tatcttcttc atgtgatcaa
     2401 tacgtttctg gatgagcgcg tcggtgccga tgtcttcacg ataagcaacg ggacctttga
     2461 cggcttcgac gccttcttca gcaatttttc gggcatccgc cagattatcg gcaattccga
     2521 caatgccgat ggcgcgcgaa gagctcaaat acaagccgtc atccttctga tccacagatg
     2581 aataatatag cctggctggc cccacattcc ccacttcaat tttcgccctg gatgaggcgg
     2641 catcgggatg gtcggcgggc aggccgtagc ccttcggcac aacatatttg caaaccgtag
     2701 ccttcttatc aaactctatt tttatttgat tcagagttcc tgcaatgatg tgtcggcaca
     2761 catccacgaa atcggttttg agaagcggca gaatattcat ggcttccgga tcgccgaacc
     2821 gtgcgttgta ttccaagagc ttgacgccgt ccttcgtggc gatgaatcct ccgtacatga
     2881 tgcctttgta gggactgccc gtttctttca aaagtgcttc tgcgacctga cgggtgattt
     2941 ccagtccgtc atgcagatcc tgcggtctca aaaaaggcat cgaatggtcc gccagtgaat
     3001 aagagcccat gcctccggta ttggggccct tgtcaccgtc aaatcttctc ttgtgatcct
     3061 gcacgagcgg agtagcgaca actgtcttcc cgtcgcacag gcattgcagc gaaaattctt
     3121 ccccgtcgaa cttttcttca atcaccagcg aagcgcctcc ggccagtatc tgtgcgcata
     3181 gtttcaacgc ttcttttttt gaatcaaaat gatctccctg gacctgaacg cccttgccgc
     3241 ccgtcagtcc gtccggtttc aaaacgatgc cttccaactg attgagaaaa tcttcgacgc
     3301 catcgagcgt tttaaatact ttaaactgcg gattaccggg aatactgtat ttgctgacca
     3361 gattccgggt gaacgatttg gacgtttcca accgggccag cgattttgtc ggtcccacgg
     3421 cgggaatatt cctttcggcc agcgcgtccg caacgccgtt gtttaaagga tcttccggcc
     3481 cgattacggc aaattcgatt ttattttccg tggcaaattt tgtaatcgcg tcaagatcgg
     3541 aataagaacc aattaaggtt ttttctgaaa gagaagcgat gcctgggtta ttggatttca
     3601 tgaaagagaa taagctgggc ttttgtccgg agcgtgtaat cgcttcagcc agtgcgtgtt
     3661 cgcgggcgcc gtttccgagc agtaatatat tgggcatggg gatctcctgt cgggcttttg
     3721 gattttttgc ttttataaag agaatagatt gggctgtcaa tggaatattc aaacgaaggc
     3781 aatgtttctt tttttgacag aatctgttaa attaataacc aaggttagag atctttgtat
     3841 aacttctctt gggtcatttc ggggagcaaa gcgacgggaa atctatattc aatattattg
     3901 ccggataaga tttctcaacg gcagagccgg atgatagcct ttggtcgaaa aaacagtttg
     3961 atgattatgc aaaagtttca attcatgaga tgaggtgtct gggtattggg ctggaattcc
     4021 gacatagttg ttttaatcct tcttttcatg ctttccggat ttttctcttc atcggaagcc
     4081 gctttgcttt cactgcggga tctgcatctg cataagatgc aacaaaacaa ttaccctttt
     4141 tttaattacg tcgtcaaact gctttccaac cagcgcaggc tgctgattac catcgtgaca
     4201 agcaacgaag tggtgaatgt cagcatttcc atactggccg cgtctttttt tatcgggctt
     4261 ctcggaagca gcggtcaatg ggtgtcgatt gtttttacaa ctgtcgtttt actttttgtc
     4321 ggcgaagcta ttcccaaaac ttttggcgtg acatacccga tgcagatttc ctcgctggtt
     4381 tctccgatct tgatggccgt ttcgcgaatc gagtctcctg tcgtttcagc cctggaaaaa
     4441 gtatccggtc ttttcctgcg atgctacggg gttaataaat ccgaggaatc ggaagtcatt
     4501 atggagcacg aattcaaaac gcttgtcgat gtcggaagtc tcgaaggtgt tgtgaatgag
     4561 gcccagaaag aattgatcaa taaaatattc gaactgggag acaggccggt tacggatatc
     4621 atggtgcccc gtgtcgacat gttttgcctt ccgctggata tgccggtagg ggacatgctt
     4681 cagtccgttg ccgccgcccg gcaggaacgg gttccggttt atcgggagga tagagacgat
     4741 atcgttggaa tactgtttgc gcgcgatttg ctcaacaatg tctggcgcgg cagactccag
     4801 accggtattt tcaatcttct ggccagaccg tttttcgtgc ccgaacagaa aacaatcaac
     4861 ggcctgctcc acgattttca gaaaaatttt attcagatcg ccattgtggt ggacgaatac
     4921 ggaggcgttt ccggcatgat tacgctggag catattctgg aacatctttt tgaagagacg
     4981 gaaggtgatg aggaaatggt ttgcgacggt tgtgaaaaaa tcgacgagca atccattatt
     5041 gtccccggga gtatgtcggt cgagcagtgc aataaactcc tggaagcaga attgccggaa
     5101 gacgagtttg acactgtagg cggactggta ctccatcttt tcggtaaaat accgaagaag
     5161 ggcgaagagg cgcatctggg cggatatgtc ttctgcgtac acgatgtcgg aaaaacaaga
     5221 atattgaagg tcaggattac gaaaaaaaca gttgaggaac ctgaagagtc atgagcctat
     5281 atttaatcgt cgcagccata ttgttttgtc tgatgctgga aatgctttat tccggcggtg
     5341 aagtagcgct ttttgcttcc gatatcaata aactgaaaaa ccgagcgcat caggggtcaa
     5401 catccgccca ccaggccgtc aagctcaaag aaaagccgga atggtttatc tcgacggcac
     5461 ttgtcgggac caatctggcg atcatcatcg cttcaacgct tatcaccggt cttttaattt
     5521 ctttttacgg tccggcacgc ggcgaaaaaa tcgctttcct gatcatgatt cccacgctgt
     5581 ttctcatgat cattgcccgc agcatatttc tttactacgc ggaagccatg gcgatgcgtg
     5641 ttgcctggtt tatccgtttt tcatcgatac ttttttatcc gatcgccttc gtgatcgcgg
     5701 ccgtttccag aagcacggtg catttctctt ccgataccag aagcgggcag tcctcgcaca
     5761 ttaccaagga aggcctcaaa tacatcttgg gcgaaaaaac aaaagggggc gatatcctga
     5821 cgcgtgagaa ggaaatggtc agccgggtgt ttgatttctc cgaacttacg gccatgaaaa
     5881 tcatggtgcc ggtttccgct ttgacggcgc tgtccgcttc catgaaaatt tccgaagccg
     5941 taggtgttgt ggccgataaa aaatacatgc gcattcccgt ttatgaagat accgtttaca
     6001 acatcgtcgg cattttacgc tacttcgatc tttttgaaat gctcctcaag gacaaggaga
     6061 aaggcgaagg tgtcggcgaa gcgacggttg cctcctgcat gcgcaaagat gtcttttatg
     6121 ttccggaaac aaaaaaggtc agcgaactgc tcgcagcaat gcaaaaaaga gacgagcaga
     6181 tggcggtggt ggtggatgaa tacggtggcg cgaccggcgt tgtcaccatt gaggatatcg
     6241 gcgaagaaat cgtcggaaca attgatgaat acgtgtcggg agaaaagctt tacagaaaaa
     6301 ttgctgaagg acgttatctt gtgtcaggca ggctggaaat aggaactctg cggcaattgg
     6361 ttaaagcgaa attccccgaa gggaattatg aaaccgtagg cggctttcta atgtatcaga
     6421 tgggtaaaat tccccgaata aaagaatatt atcagtatgg cgatgtgacg tttattattg
     6481 aaaacgccga ccagaaatcg attaaggaag tgacggtaat cgcgcctttt attcaagagg
     6541 ggaacaaata aatatctttt tgtcattccc gcgcaggcgg gaatccagta aatgtttttg
     6601 ttcaaaaaag attattaata attggagtga ttttgataag tgtagatgag ataatcaagg
     6661 attattaaat gaaatcagat ttaacaagac aagattggga agagtattta atccgtctct
     6721 attttgggac caaaaatgat ttaataaaat catgtatcaa tagagcctat cgcgatttca
     6781 atagaaccct tcatggaatg gatgatcaca aaaataaaca agatttattt gatagagccg
     6841 taaaatatct gctctcaatt ttcatagata taaaaaaaga gtcattaaat gaccacgaag
     6901 aatttgataa atggcataaa gaaaaatgcc ttcatttaaa aaatttatat aaagaaaatg
     6961 atttcattaa tttttccatt gggcaggcac agaaatggat aaacatgact tttaagtata
     7021 tattttgtgt gggtgagaaa agattacccg gttattctgg tttatatgag ttttgtcatg
     7081 ttcctattga caatataatt ttagataaaa ttaaaaaaag aggtgctcct gtcccgtcaa
     7141 aaccgtggag tcgacttggt gattataacg aatatatctc tctccagaag tggttccgag
     7201 aacaatataa attaccattg gatggtgaat ttatattatg gatggaggaa cctcatgata
     7261 atgtcattat gcctgttcaa aacaatattg ttttggataa aaccagagtc aaagaagcaa
     7321 aagacgagac cataggacca tatcaactta acagaataat tctgtgctct gccgatgcga
     7381 gtccggcatc acaaaagaaa gacaaatata aagcagaata tttcttccca agcgcaaagt
     7441 gggttggagc cgttagaaat gtcgcaagtg atttgggatg taaatttgtt attcttacga
     7501 ccaaacatgg gctggttaat ccttccgatg atattgatca ttacgatcta gaaatcaaag
     7561 gtaatgaaaa tattattaat gagaaatggc aagatacaac gtcaagatta attagaagag
     7621 gacaatatga cattctagtt ttttacgcag gtggatgtcc tcgggatgaa tacttgagaa
     7681 tattaagtcc gattcttaag aaccttgaaa tagatttatt aacgtttggg aagcccaaca
     7741 tgggagatgc gggaaaaatt aaagacatcg tcgaatcgtt gggggaagaa gcatctatag
     7801 atacattgaa aaagaaactt aaattcccag atagactatt atttttcccc cacaataata
     7861 aggcataatt aattttaaaa tactggatac cggcctgcgc cggtatgaca aacgaataag
     7921 gattgataag gtgtaggaaa gtgcgttgat attggcggcg gggaagggta cccgcatgaa
     7981 ataaatccgg gcttgcaaaa ttttgcaaag tatatataat taacaaaagt gataactata
     8041 tatgcttagt aaaaatatgg cagaattgca ggaattgagc aggaaaacct gctttaccct
     8101 cgaagatgtg gcgcagagtt tgggaatgca aacggcatcc gcccgcgtat tgtgcagccg
     8161 ctatgtccgg cagggggtgc tgataagatt gaaaaaaggt ttttatacca ccgcctggaa
     8221 gtgggaaaat cttaatcgtc aggatttttt caaaatcgcc aatatcctgc aggttccttc
     8281 ctatatttcc ctgatgtcgg ctctggctta ttatgaagta acgacgcagg cgcagaacaa
     8341 ctatcaggaa aatatttgcc tgaaacgttc gattgtttac aatgtaaggg aagcggtctt
     8401 caattatgtc aaagttcagg cccgatacta tggtgatttt gttaaaacgg atggcatctt
     8461 catcgccact aaagagaaag cgtttgtgga cgctgcctat ctttattcgt tcggcaaata
     8521 taagttcgat gttgattctc tggatatgaa gaagctggat atcaagaagc tcaggagcat
     8581 catcaaaatc tatccgcaaa aaacaaaaga gacggttaaa agattatgct ggatttgatt
     8641 caacatgaac aatttgagct ggaagtttta aacagacttc aaagcgggcg gtttctggcc
     8701 aacttgattt tcggcggcgg caccatgttg cgcttgtgtc atggactgaa tcgttattca
     8761 gttgatctgg atttctgggt agttagggag atggattggg caaaatacta tagccggatg
     8821 gaaaaatatc tgggccagtt ttacaaaata gccgattccg ccaataagca ttttacaatt
     8881 ctgttcgaat tgaaatcgcc gcagtttcca cggtcgctga aaattgagat acggaaaacg
     8941 gtaaagaacg tcaagacaga aacgagcatc gcctacagtc cgcacgcgcc ggttcaggtg
     9001 atgttgaaaa ccgtggcgct aaaagatatg atgacttcaa aaatcgaagc ctttcttgac
     9061 cgccgggaaa tacgcgacgc atatgatata gaatttctag taaaacgcgg gattgttttg
     9121 aatgccgatc atgaggttat cacccaaatg cttacgaaac tggggaaatt gggcaaaaag
     9181 gatttttcag taaagctagg ttcgttgctt gatgtctcgg aacgaaagta ttatgtggaa
     9241 aacggattcc gcattctgga ggcgaacctg agggataaac tcaggtagaa tttaaaatac
     9301 aggataccga ccttcgttgg tatgacgatt gaaggagatg tttgataagg tgaacgaaac
     9361 gaaattttgt gcgttgatac tggcggcggg gaagggtacc cgaatgaaat cggataaggc
     9421 caaggttctg catgagttga acggaaagcc cctgctttat tattccatcg aagcggcaag
     9481 agcggttggc gtcgaaaaga ttgtagccgt gatcggccat caggcggaga gagtcaggga
     9541 aacatttgcc gccaacggct gtatttttgt cgaacagaat ccgcagttgg gaacggggca
     9601 tgccgtttta caggcgaagg atgtactgac tgattacaaa ggctttaccg taattctttg
     9661 cggcgatgtg ccgcttttaa aaccggcgac aatcaaatcg ctggtggata atcatgtcgc
     9721 cgaaaaagcg gtcgtttctg tgttgacaac cattccaccg catcctcatg cctatggccg
     9781 catcgtaaag gacagcaagg gcaatgtttt gaaaatagtc gagcataggg atgccacgga
     9841 agaggagaaa aaaatcgggg aaatcaatac cggcatttat tgtgtggata cagagttttt
     9901 gtttcatgct ttgggaaaag tgaccaataa caaccggcaa caggaatatt atctgaccga
     9961 tattgttgaa atagccgttt gtgaaggcca gaaggtaaaa tcatttatcg ctgacgatta
    10021 cgttgaagtg atgggaatca acacgctgga agaactcacc cgcgcggaaa agtatctccg
    10081 aaaaggctga aaattttctg ttttatttgt ttctgtcctg atattaacat taaagtgaaa
    10141 caattatttt tcggtcatac cggtcgcaga tgaccttcag gttacgaagg ccggccatgg
    10201 tgcccctata gggtatatcc agttattctt ggatgccgtt cttcaacggc atgacatagt
    10261 tgtgcgtgat attttatttt tcagtcagct aggtaattat tggtttgaaa aatataaaca
    10321 acataatcga agcagtcgca aaaaaaggag ctctgcttgc gcctctggcg ggaatttccg
    10381 atttaccttt ccgtttaatc gcccgatctc aaggatgttc cctggcctat acggagatga
    10441 tcagttccaa tggtcttgtc cgaaacacca gaaaaacgta cg
//
LOCUS       DAOOLB010000072        10161 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_7563_length_10161_cov_44.196220, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000072 DAOOLB010000000
VERSION     DAOOLB010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7563_length_10161_cov_44.196220"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PLF77_08610"
     CDS             <1..389
                     /locus_tag="PLF77_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPC08952.1"
                     /translation="GTRKAIQIPFVLLTSNNYRDMSDALKRRCVHLYIDYPARDTEIS
                     IVRLKIPGIEEKLCDALVDAIRKIRKLDLKKNPSISETIDWAQSLIALQINELTPEVV
                     SETLNIICKYQADMEKVRKNINRVLQ"
     gene            629..1960
                     /locus_tag="PLF77_08615"
     CDS             629..1960
                     /locus_tag="PLF77_08615"
                     /inference="COORDINATES: protein motif:HMM:NF017569.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPC08953.1"
                     /translation="MRISTSEVLDSLHQMKLINPVDEQQFRSMLRSNFAKSMREVQHF
                     DRLYHLFFHEMRIDTSDIRQAADYSTEINMTGDALKKIPHDNPIYDAVVDFLQGNPMA
                     LLREMRRIRTEEDVTMLRFNLGPLGNRLNVMLQINKAASDARTLVEDHYRKTDASAGR
                     KLAAYFEERVSAARSMLVYEPQQGESGTRKTKTEEQKLKGLGEKSFSSFTRQEVEEMR
                     EAIDKMVRKLKNIVSRRYAVRNRGSIDIKKTLRASAKYNGVPVDVKYRSRAKRKSRIV
                     TLCDVSGSVWAAARFMLNLLYALQDCFDKVNSFVFIDHLSDVTSIFEKNEINEAIRLV
                     LEKPDINFNASTDYGETLRNFKKDYMDLLTKKTTLIIVGDGRTNYMNPEDAILGQMRE
                     RCRRIIWLNPEQENLWSSGDSEIGSYMPHCHEVRQCRNVNQLFSFIEELVL"
     gene            1962..3086
                     /locus_tag="PLF77_08620"
     CDS             1962..3086
                     /locus_tag="PLF77_08620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPC08954.1"
                     /translation="MQTTTKDQSYSWISRFAIEHVASRNDLQTMQDFPLDIWQKMGEA
                     GFFKLGISPQFGGSGGGLTQLAGAGEVFVRSGFNMGLALSWLYQQIIAIHLMSGFGTE
                     EQKKRYLPLMANGSLTVSFAVSEPKHGARPKLLTSTADLQDTSYILNGEKTYLTNAPI
                     AGLFIVIAITGRDKDQKRFSAFLVPADHEGLMIKPRLKFDFLKPAPHGGIKLNNCRIP
                     ASAILGEKDSAYANIVIPFSDAENTVLMGAVTGGMGAELMDLIMESDKRNIHKDKALQ
                     SEVGALDSLLETMRAIVRETAKKVDERDNAAVPLIVTFKELAAGFQARIAAAIAKWDI
                     KTPDRFKVLQTDINVLGALQEKTLQDKQAKLGDELLHGAR"
     gene            3129..3608
                     /locus_tag="PLF77_08625"
     CDS             3129..3608
                     /locus_tag="PLF77_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPC08955.1"
                     /translation="MMTLLWILAVLFILIGVAGTLIPALPGVPLILIGVLIGAWIDDF
                     QRISAATVVVMVILTVLGVVIDYVAAALSAGRAGASRQGLIGAVLGTLAGLFTGFWGL
                     LFMPLAGAAIGEFIARRDMLHAGKVGAATWFGLVISVVVKLAIAFTMVGVFIAALLI"
     gene            3658..>4403
                     /locus_tag="PLF77_08630"
     CDS             3658..>4403
                     /locus_tag="PLF77_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPC08956.1"
                     /translation="MNEKLLKPYRIFLKDTIRKVTDFSQTDQSRGIPPPPIEKPYSGT
                     ARRLDLVRYDQFQNIGTLDLKTAIRNRESRRTYGTQPLTLGELSFLLWATQGIKQILD
                     AGHALRTVPSAGCRHALETYLCILNVQGIAEGIYRYLPLEHQLLHEQTVENLKLKIRQ
                     AVFGQRYPADAAATFIWTAVPCRMEWRYGIAAHKVIALDAGHVCQNLYLACEAIGAGT
                     CALAAYDQEEMDRLLSIDGEEEFTIYLASVG"
     assembly_gap    4404..4503
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4504..6451)
                     /locus_tag="PLF77_08635"
     CDS             complement(<4504..6451)
                     /locus_tag="PLF77_08635"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HPC08957.1"
                     /translation="MLNKTPADGGLKKTVVAVGGMTCAACVRRVENAIKDVIGVRDAV
                     VNLANGHATVMHSTQWEGPAALARVVAEQGYEYLGEIKNHLADPIEASRLKELRELKL
                     KVACGAVLSVIIFFGSMQHWFPFLDFIPHRIMLLLLFVLTAPAVFWVGSRFFIGAYKA
                     ALQKTSDMNTLVAVGAFSAYAYSAAATFSPQFFTTAGITPHVYYDGAAMIVTLILLGR
                     LLEAGAKSKTSMAIKNLIQLKPKTAHLIREGQELDVAVDEVRIGDVVFVRPGERIPVD
                     GTILSGESTIDESMLTGESIPVSKVAGQKVFAATMNTTGGFTFQATGVGAETALAQII
                     RLVQEAQGSKAPIQRLADKVVSVFVPAVFLIALMTFAVWYFLSAEGNFSRALISFVSV
                     LVIACPCALGLATPTAIMVGTGLGAQSGILIKGGEALEKIHKLSVIVFDKTGTLTKGE
                     PDVTDILTADGYEEKQILSFAAALEKSSEHPLARAIVRKAEQKSVRIETVEKFTALSG
                     LGAKALMDGQRVLIGNRFLMTDEGIAIDDWNLSTERLTGEGKTVVYLALGKSVVGLIA
                     LADTPKASAARTVQRLQSRGMKVAMITGDNDGTARAIARQLGIEKVMANVLPGKKAEE
                     IKNLQDAGEIVAMVGDGINDAPALA"
     gene            complement(6441..6641)
                     /locus_tag="PLF77_08640"
     CDS             complement(6441..6641)
                     /locus_tag="PLF77_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPC08958.1"
                     /translation="MKSIKIKGMSCGHCVMAVTKALNAVDGVRNVEVDLKNGVATYEE
                     IKPVDSAVVAAAIKKAGYDVVE"
     gene            6867..7709
                     /locus_tag="PLF77_08645"
     CDS             6867..7709
                     /locus_tag="PLF77_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC08959.1"
                     /translation="MIIRCWGSRGSIPVSGRLYQRYGGNTTCLEIRTKDNNILIVDAG
                     SGIREAGNALLAEGSLNFTLLLTHAHWDHIMGFPFFKPIYSSKIRMDIRGCPFAQTSI
                     KKMLSRIMAAPNFPVNYSHIRAKIVYHETCFKPVYRLGDMLITPIALSHPNGGTGYKF
                     EEDGKTFIFLTDNELGHRHPGGLPFRQYLAFCRNADLLFHDAEYKEAEYKKTRGWGHS
                     SYKDVLKLSLGARVKKLGLFHHNQERTDPDVDAILKDCRDTVKKEGGKMNCVAVGQSM
                     QFTL"
     gene            7740..8132
                     /locus_tag="PLF77_08650"
     CDS             7740..8132
                     /locus_tag="PLF77_08650"
                     /inference="COORDINATES: protein motif:HMM:NF015166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08960.1"
                     /translation="MLFLLHIVLMVIATLGIMAGVSAAMFFRRKKNWLKIHKTLNTLS
                     LAGITVGIIMAFFYVAGTGGKHLNDVHQMTGLAAFTVVLIAIALGHYQFKALNKSAIR
                     AAHRWMGRVSIFILAAAAFLGLRLINLL"
     gene            8168..9865
                     /locus_tag="PLF77_08655"
     CDS             8168..9865
                     /locus_tag="PLF77_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HPC08961.1"
                     /translation="MFLPTTRDELKKLKWDKPDIILVTGDTYIDSPFIGVSVIGRVLV
                     DAGFRVGIIAQPDWHKPDDISRLGEPRLFWGITGGCMDSMVANYTATKKKRGNDDLTA
                     GGKNNRRPDRAVVVYANLIRRYFKETKPLVLGGIEASLRRIAHYDFWSDSIRRSIMFD
                     ARADVLVYGMAEKAVVELAQALRDGKDTTDIRGICYLASAPPPQNALELPAYEDVLAD
                     KKTFIRMFNRFYCNGDPLTAKRLYQKHGDRYLVQNPPQPHLTTPELDHIYGLDFTRDV
                     HPFYSARGNVRAIETITFSITSHRGCYGECNFCSIGLHEGRTVISRSEKSILDEAQKL
                     AGRSDFKGYIADVGGSTANMYGIECAKKIKKGACNGKRCLSPRVCDALPADHSRQINL
                     LRNLRNVRGVKKVFVASGIRYDLLLNDRAHCLAYMKELVEHHTSGQLKVAPEHSEWPV
                     LKLMGKPQAQALLNFKHLFEKTSAVSKRKQFLTYYFIAAHPGCTEEDMRRLKAFTTRE
                     LKTNPRQVQIFTPLPSTYSALMYFTGIDPSTGKKIFIEKNMEKKEKQKNILIGNKRS"
     gene            complement(9895..>10161)
                     /locus_tag="PLF77_08660"
     CDS             complement(9895..>10161)
                     /locus_tag="PLF77_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC08962.1"
                     /translation="TIALDQNHARAFHDRGLAYFHLGRYEQAISDYDGAIRRNPDFTL
                     AYHNRAYGYFRQRNDVRGCPDAHRACALGDCDLLTAVQQEGRCR"
BASE COUNT         2488 a         2719 c         2518 g         2336 t
ORIGIN      
        1 tgggcacccg caaggccatt cagattccct ttgtgctgct gacatccaat aactaccgcg
       61 atatgagcga cgcgctgaag cgacgctgtg ttcatctcta cattgattat ccggcgcggg
      121 acacggaaat ttccattgtg cggttgaaga ttccgggcat cgaagaaaaa ctctgcgacg
      181 ctcttgtgga cgcaatccgc aaaattcgca agctggattt gaaaaaaaat ccgagcattt
      241 cggaaacgat tgactgggcg cagtcgctga ttgctctgca aatcaatgaa ctgacacctg
      301 aagttgtttc ggaaacgctg aatatcatct gcaaatatca ggcggatatg gaaaaagtcc
      361 gtaaaaacat caaccgggtc ctgcagtagt ttaaaatgtc attccgaatt ccgacgtctc
      421 gggatgagaa atctttacga tgctgatatc tgaagacttc tccccaacgt agcaaaacga
      481 tagccttcag tcgaattgac ataacgctta tcgtggagta ggatcccgta tatgcggacg
      541 gctaaatgac agaacaatcg attattttta tctgtaattt atggtcgggc ttattcaaca
      601 atttgtttcc tgctgcaggg cgtcgggcat gaggatttcg acatccgaag tcctggacag
      661 cctccatcag atgaaactga tcaatccggt ggacgagcag caattccggt cgatgcttcg
      721 ttccaatttt gccaaaagta tgcgcgaggt tcagcacttt gaccggctgt atcatctttt
      781 cttccatgaa atgcgcatag acacatccga tatccggcag gcggccgatt attccaccga
      841 aatcaacatg acgggagatg ctcttaaaaa aatcccccat gacaatccga tatacgatgc
      901 cgtagtggat tttttgcagg ggaaccccat ggcgcttcta cgggaaatgc gccggattcg
      961 aacggaagaa gacgtgacca tgctgcgttt caatctggga ccgctgggca accgccttaa
     1021 tgtgatgctg caaatcaaca aggccgcgtc cgacgcccgg accctggtgg aagatcatta
     1081 caggaaaacg gatgcttcag ccgggcgcaa gctcgccgcc tatttcgaag aacgggtaag
     1141 tgccgcccgt tccatgctcg tttacgaacc gcaacaggga gaatccggca cccggaaaac
     1201 aaaaacggaa gagcaaaaac tcaaaggact gggcgaaaaa tcgttttctt ctttcacccg
     1261 gcaagaagtt gaagaaatgc gcgaagccat cgacaagatg gtgcgcaaac tgaaaaatat
     1321 tgtcagccgc cgatacgccg tccgcaacag gggaagcatc gatatcaaaa aaacactgcg
     1381 cgcatcggcc aaatacaacg gggttccggt ggacgtcaaa taccgcagcc gggcgaaacg
     1441 caaaagccgc attgtcacgc tctgcgatgt gtccggttcc gtctgggcgg cggcgcgttt
     1501 catgctgaat ctgctttacg ccctgcagga ctgctttgac aaggtcaaca gttttgtgtt
     1561 tattgatcat ctttcggacg tcacgtccat ttttgaaaaa aatgaaatca acgaagcgat
     1621 tcggctggta ttggaaaaac cggatatcaa tttcaacgca tcaaccgatt acggggaaac
     1681 gctgcgcaat tttaaaaagg attacatgga ccttctgacc aagaaaacaa cgctgattat
     1741 tgtcggcgac ggccgcacca actacatgaa ccccgaagat gctattttag gccagatgcg
     1801 tgaacgctgc cggcggatta tctggctcaa tcccgagcag gaaaatctct ggagttccgg
     1861 cgacagcgag atcgggtctt acatgccgca ttgtcatgag gtgcgtcagt gccgcaatgt
     1921 caaccaatta ttttctttta tagaggaact ggttttataa aatgcagacg acaacaaagg
     1981 atcaatcgta cagctggatt tcacgttttg ccattgaaca cgtcgcttca cgcaacgatt
     2041 tgcagacgat gcaggatttt ccgctggata tctggcaaaa aatgggagaa gcgggatttt
     2101 ttaaattggg catctctccg caattcggcg gcagcggagg aggattaacg caactggccg
     2161 gcgccggaga ggtattcgtc cgcagcggtt tcaacatggg actggctctg tcctggcttt
     2221 atcagcagat catcgccatt catctgatgt ccggtttcgg cacggaagaa caaaaaaaac
     2281 gttatctgcc cctgatggcc aatggaagtc tgacggtttc ttttgccgtt tccgaaccga
     2341 aacacggggc gcggccgaag ctcttgacgt ccacagccga tttgcaggat acatcctaca
     2401 tcctcaatgg agagaaaaca tatctgacca atgcgccgat tgccggcctt ttcatcgtga
     2461 tcgccatcac cggccgggat aaagatcaga agaggttcag cgcctttctg gtgcccgccg
     2521 atcatgaagg cctaatgatc aagcccaggc ttaaattcga tttcctgaaa cctgcgccgc
     2581 acggcggcat caaattaaac aattgccgca tccccgcttc cgccatcttg ggagaaaaag
     2641 actccgccta tgcaaatatc gtcatcccgt ttagcgatgc ggaaaacacg gtgctgatgg
     2701 gcgcggtcac cggaggcatg ggcgcggagc tcatggatct gatcatggaa agcgacaaac
     2761 gcaacattca taaagataaa gcgctgcaaa gcgaagtcgg cgcgctggat tctctgctgg
     2821 aaacgatgcg cgccattgtc cgtgaaaccg ccaagaaggt tgatgaacgc gacaatgccg
     2881 ccgttccgct gatcgtcacc ttcaaagaac tggcagccgg atttcaggcc cgaatcgccg
     2941 ccgccatcgc caaatgggat attaaaactc cggaccgctt caaggttttg cagaccgata
     3001 ttaacgtcct gggagcattg caggaaaaaa cccttcagga caagcaggcg aaactgggcg
     3061 atgaactgct tcacggcgcg cgataaagat tgttccatcg attcgaagag ggttgagtct
     3121 ttcatggtat gatgacgttg ttatggatcc ttgctgtttt gttcatcctg atcggcgtcg
     3181 ccggaacgct gataccggct cttccgggcg ttccgctgat cctgatcggc gtgctgatcg
     3241 gcgcctggat cgacgatttt cagcgcatca gcgcagcgac agttgtggtg atggtgatcc
     3301 tgacggtcct cggtgtggtg attgattacg ttgccgcagc gttatccgcc gggcgcgcgg
     3361 gggcaagccg gcagggactg atcggtgcag tcctgggaac cctcgcgggc ctgtttaccg
     3421 gattctgggg gcttttattc atgccgctgg ccggggccgc catcggcgaa ttcatcgccc
     3481 gtcgggatat gttgcatgcg ggaaaagtcg gtgccgccac ctggttcgga ctggtcattt
     3541 ctgttgtggt gaaactggca attgccttca cgatggtcgg cgtgtttatc gctgcgctgc
     3601 tgatctgaga aaagattgac ccggccgcaa aaccggctcc ggatacagga tgcagccatg
     3661 aatgaaaaac ttctgaaacc ctacaggatt tttctcaaag ataccatccg taaagtcact
     3721 gacttttctc agacggatca gagcagaggg atccctcctc cgcccatcga aaaaccctac
     3781 tcaggcaccg cccggcgcct tgatcttgtg cgctatgacc agtttcaaaa catcggcaca
     3841 cttgatctta aaacagccat ccgcaaccgc gaaagcaggc gaacctacgg cacgcaaccc
     3901 cttaccctcg gagaattgtc ttttctcctg tgggcaacgc agggaatcaa acaaatcctt
     3961 gacgccggtc acgccttgag aacggttcct tcggccggtt gccgtcacgc tctggaaacg
     4021 tatttgtgca ttctgaacgt tcagggaatc gctgagggca tttaccgtta tctcccgttg
     4081 gagcaccagc tgttgcatga acagaccgtg gaaaatctaa aactaaaaat ccgtcaggcg
     4141 gttttcggcc agcgttatcc cgcagacgcg gcggctacat tcatctggac ggccgttccc
     4201 tgcagaatgg agtggcgtta cggtattgcc gctcacaaag tcatcgcgct tgatgccggt
     4261 catgtctgcc agaatcttta ccttgcctgc gaagctatcg gcgccggaac ctgcgctctg
     4321 gccgcttatg atcaggaaga aatggaccgg ctgctcagca tcgacggcga ggaagagttc
     4381 acgatctatc tggcctctgt tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnccgccag cgcaggcgca tcgttgatgc cgtcgccgac catggcgaca atctcgccgg
     4561 catcctgcag attttttatt tcctccgcct ttttccccgg caggacattg gccataactt
     4621 tttcaatgcc caattgtctg gcgatggcgc gggccgtacc gtcgttgtct cccgtaatca
     4681 ttgcgacctt cattcccctg ctctggagac gctggaccgt ccgcgcagcc gatgctttcg
     4741 gcgtatcggc cagggcaatc aaacccacaa ccgattttcc caaagccaga taaacaaccg
     4801 tctttccttc tccggtcagc ctttccgtag aaaggttcca gtcatcaatg gcaatgcctt
     4861 cgtctgtcat taagaaacgg ttgccgataa gaacacgttg gccgtccatc agggctttgg
     4921 ctcccagacc ggacagcgcc gtgaattttt caaccgtttc gattctcacg cttttctgtt
     4981 cggctttccg cacaatggcg cgggccagcg ggtgttcgga acttttttcc agggctgcgg
     5041 cgaacgacag aatctgcttt tcctcataac cgtccgcggt caaaatatcg gtaacatccg
     5101 gttctccctt tgtcagggtg cccgttttat caaaaacgat caccgaaagt ttatggattt
     5161 tttccagcgc ttcgccgcct ttgattaaaa tgccgctttg cgctcccagg cctgtgccca
     5221 ccatgatggc tgtcggtgtc gccagtccca gagcgcaggg acaggcaatc accagcacgg
     5281 aaacgaaact gatcaaggca cggctgaaat tcccttccgc ggaaaggaaa taccagacgg
     5341 caaaggtcat caaggcaatc agaaacacgg ccggaacaaa aacggagaca accttatccg
     5401 ccagacgctg aatcggagcc tttgatccct gagcttcttg caccagacgg atgatttgcg
     5461 ccagcgcagt ttctgcgccc acgccggtcg cctgaaacgt aaacccgcct gttgtgttca
     5521 tcgtggcggc aaaaaccttt tgtcccgcca ctttactaac cgggatactt tcaccggtca
     5581 gcatggattc gtcgatggtc gattcccccg acagtatcgt gccgtccacg ggaatccgtt
     5641 ccccgggcct gacaaaaacg acatcgccaa ttcgtacctc atccacggcg acgtcgagtt
     5701 cctgaccctc ccggatgaga tgcgctgttt tcggctttaa ttgtatcagg ttttttatcg
     5761 ccatggacgt cttgctcttc gccccggctt ccagaagtct gcccagcaga atgagcgtga
     5821 caatcatggc cgcaccgtca taatacacat gcggtgtaat gcccgccgta gtaaagaatt
     5881 gaggggaaaa agtcgcggcg gccgaatagg cgtaagctga aaaagcgccg acggcaacca
     5941 gtgtgttcat gtcggatgtt ttctgaaggg cggctttgta ggcgccgata aaaaagcggc
     6001 tgcccaccca gaagaccgcc ggggcggtca aaacgaacag cagcaggagc atgatccgat
     6061 ggggaatgaa atccagaaaa ggaaaccagt gctgcatgga accgaaaaaa atgatgacac
     6121 ttaaaacggc tccacaggca actttcagtt tcagttcccg gagttcttta agacgcgacg
     6181 cttcgatggg atcggcaaga tgatttttga tttcgcccaa atattcatac ccctgttcgg
     6241 cgacaaccct ggcaagcgcc gccgggcctt cccattgcgt cgaatgcatg acggttgcat
     6301 gtccgttggc aagattgaca accgcgtctc gcacaccgat tacgtctttg atggcatttt
     6361 ccacgcgccg gacacaggcg gcgcaggtca tgccgcccac ggcaacaacg gttttcttga
     6421 gccccccgtc cgcaggcgtt ttattcaaca acgtcatacc ccgctttttt gatcgctgcc
     6481 gcaacgacag cggaatcgac aggttttatc tcctcataag tcgccacacc gtttttcaaa
     6541 tccacttcaa cattccggac gccatccaca gcattcaggg ccttcgtcac cgccatgacg
     6601 caatgcccgc aactcattcc tttgattttt atgcttttca tttcatcacc ctccctatat
     6661 tcattttagt ttaataattt ttttgataat agcaaggtga aacagggttc tttcgggatt
     6721 ttcccagaaa cgttttgtcc tgatatcctg cttgatttaa aatgtattct gtattattga
     6781 aacccctata gaaaaaaatt atcctccatc gtgatccacg gggatattga taggcgacat
     6841 tttcatcttt ttttacagga ttaacaatga tcattcgctg ttggggttcc agagggtcca
     6901 tccctgtatc gggcagactg tatcagcgct acggcggcaa taccacctgc cttgaaatca
     6961 ggacaaaaga caacaacata ttgattgtcg atgcaggctc cggaatccgc gaggcaggca
     7021 acgccctgct ggcggagggc agtttgaatt tcacgcttct gctgacgcac gcccactggg
     7081 atcacatcat gggatttccc ttcttcaaac ccatctattc ctctaaaatc cgaatggaca
     7141 tccggggatg tcccttcgcc cagacctcga taaaaaaaat gctttcccgg attatggcgg
     7201 ctcctaattt tccggtcaat tacagtcaca ttcgtgcaaa gattgtatac cacgaaacct
     7261 gtttcaaacc cgtctatcgg ctgggcgata tgctcatcac ccccattgcc ctgagtcatc
     7321 ccaacggcgg aaccggttac aaattcgagg aagacggcaa aacgttcatc tttctcacag
     7381 acaatgaact gggtcacaga caccccggag gactcccgtt caggcaatat ctggcatttt
     7441 gccgaaatgc cgatctgctc ttccatgacg cggaatataa agaggcagaa tacaaaaaaa
     7501 cccgcggttg gggacattct tcctataaag atgtcttgaa actgtcgctt ggggcccgcg
     7561 tgaaaaaact gggactgttt catcacaatc aggagaggac cgaccccgat gtcgatgcga
     7621 ttctgaagga ctgccgcgac accgtcaaaa aagaaggcgg gaaaatgaac tgtgtggccg
     7681 tcggccaatc catgcagttt acgctttaat ttttcaacgc gttttataaa caagatatca
     7741 tgcttttttt attacacatt gtcctgatgg tgattgcaac gctgggcatc atggccggag
     7801 tgagtgccgc catgtttttc cgcagaaaga aaaactggct gaaaattcat aaaaccctca
     7861 acactttgag cctggcggga atcactgtgg gaatcatcat ggcatttttc tatgttgccg
     7921 gcaccggcgg aaaacatctc aacgatgttc atcagatgac cgggctggca gcgtttaccg
     7981 ttgtcctcat cgccatagct ctgggacatt accagttcaa agccctcaac aaatcggcca
     8041 ttcgggcggc gcaccgctgg atgggacgcg tttcgatttt catcctggca gccgccgcct
     8101 ttctgggatt gaggctgatc aatcttctct aaccggccaa tgccgcaatc gtgagccgtt
     8161 tttcgttatg tttcttccca caacccgcga cgaattaaaa aaattgaaat gggacaaacc
     8221 cgatatcatt ctggtgaccg gtgacaccta catcgacagt ccttttatcg gcgtgtccgt
     8281 catcggccgc gttctggtcg atgccggctt tcgtgtaggc attatcgctc agcccgactg
     8341 gcataagccg gacgatatca gccgactggg cgagcctcgg cttttctggg gaatcaccgg
     8401 cggctgcatg gattccatgg tcgccaacta cacggcgacg aagaaaaaaa ggggaaacga
     8461 tgatctgact gcaggcggaa aaaacaaccg tcgtcccgac cgcgccgttg tggtgtacgc
     8521 caatctgatc cgccggtatt ttaaggagac caagccgctg gtactcggcg gtatcgaagc
     8581 ctccctgcgc cgcatcgccc attacgattt ctggtcggat tccatccgcc ggtcgattat
     8641 gttcgatgcc cgtgccgatg tgctggtcta cggcatggcg gaaaaagccg tcgtcgaact
     8701 cgcgcaggct ctgcgcgacg gaaaagacac aacagatatc cggggcatct gttatcttgc
     8761 gtccgccccc ccgccgcaaa acgcactgga actgcccgct tatgaggatg tgcttgcgga
     8821 caagaaaaca tttatcagga tgtttaacag gttctattgc aacggcgatc cgctgacagc
     8881 gaagcgcctg tatcaaaagc acggcgaccg ttatcttgtg caaaatcccc cgcagccgca
     8941 tctgacaacg ccggaactgg atcatattta tggacttgat ttcacgcgcg acgttcatcc
     9001 cttctacagc gcccggggca atgtcagggc gatagaaacc attacgtttt ccataacgtc
     9061 tcatcgcggt tgctacggtg aatgtaattt ctgctccatc ggtctgcatg aaggacgcac
     9121 cgttatcagc cgttcagaaa aatccattct ggatgaggcg caaaaactgg ccggacgttc
     9181 cgattttaaa ggctatattg cggatgtcgg cggatcgacg gccaacatgt acggcatcga
     9241 atgcgcgaaa aaaatcaaga aaggggcctg caacggcaaa cgatgtctgt ccccgagggt
     9301 atgcgacgcc ttgccggcgg atcacagccg gcagatcaat ctattgagaa atctgcgaaa
     9361 tgtcagaggc gtcaaaaaag ttttcgtggc ctccggcatc cgatacgatc tgcttttaaa
     9421 cgacagggca cactgcctgg cgtacatgaa agaactcgtc gagcatcaca cctccggtca
     9481 gttaaaggtc gcgcccgaac atagcgaatg gccggttctg aagctcatgg gcaagccgca
     9541 ggcgcaggcc ctgctgaatt tcaaacatct ttttgaaaaa acatccgccg tttcaaaacg
     9601 caaacagttt ctcacctatt atttcattgc cgctcatccg ggatgcacgg aagaggatat
     9661 gagacgtctc aaagcgttca ccacaagaga gctaaaaacg aatccccggc aggtacagat
     9721 attcaccccg cttccctcga cctattcggc cctgatgtac tttaccggta tcgatccttc
     9781 gaccggcaaa aaaatcttta tcgaaaagaa catggagaaa aaggaaaaac agaaaaacat
     9841 cctgatcggg aataagaggt cataaccccg aaagcgcacc ggttttgccg gcactcagcg
     9901 gcaacgcccc tcttgttgaa cagcggttaa caaatcacag tcccccaatg cacaggccct
     9961 gtgtgcatcc ggacaacccc gaacatcgtt tcgctgcctg aaataaccat acgccctgtt
    10021 atggtaggcc agagtaaaat ccgggtttcg tctgatcgct ccgtcataat cgctgatcgc
    10081 ctgctcatac cggccgagat gaaagtaagc aagtccccgg tcatgaaagg ccctggcatg
    10141 gttttgatcc agggcgatgg t
//
LOCUS       DAOOLB010000073         9675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_8172_length_9675_cov_49.299896, whole genome shotgun sequence.
ACCESSION   DAOOLB010000073 DAOOLB010000000
VERSION     DAOOLB010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9675
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8172_length_9675_cov_49.299896"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PLF77_08665"
     CDS             complement(<1..1073)
                     /locus_tag="PLF77_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08963.1"
                     /translation="MKKYSSVEKMAPVGFVLLGAGSVGGGVFHQSLLTRDVDCKVICD
                     KAMEKVMALEDPRRRFEIVSTEGELADALNAGKVAVCEDAQLAAIAPGVEVFFDASTA
                     VDSASRTIESAMRSGKHVVMMNAEADAVFGPWFWHLAQKYGVAYTSSDGDQPAVIARL
                     VEELRFYGLDFVMAGNIKGFLDRYTDPVKITPEADKRFLDHKMCSSFTDGTKLCVEMS
                     IVANALGCSVVQPGMLGPKMNETIELFDHFPLSDLWTPGQAPLVDYVLGARPKGGVFA
                     VGYTDDAYQRRMLDWFPSETGPGPFYVFTRPYHLIHFETMRTILEVARGHRSLMAPRF
                     GMRTEVICYAKKVLEPGHLLDGPG"
     gene            1552..2832
                     /locus_tag="PLF77_08670"
     CDS             1552..2832
                     /locus_tag="PLF77_08670"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPC08964.1"
                     /translation="MIEFIGYFCAFLAIASSLIGLLSVFIFRSHIKHWTRKTPVTYPA
                     LTVISPQRGKIDPENVEAILEQNYPGVWEIIFVTTKDDASLPQLQHYLQKYKNVKIAI
                     AEDVVELATTKGIHRCQKNNNLLTAIKAASPQTEVYAIIDADARPFSDWLSCLVAPLT
                     DANSRLGVVTSARIYLPGKGLASLVQVLWILISAMFLVGKCRYIWGGGLAIPKKVFDN
                     ANLARQINGQGGSSITTDDMNIYNALRKQGYESLFVPDCIVLRHPPKHKESLFNVIGF
                     TNRQVLQTWWTTRGIWVLLCNIGIRLPMLLCALVIAWWYPWCLLALLSIVIDVFMGIS
                     ALKTVIDAEPRLSAKLIFWIMKSKGALAGRSLQPEIRIQVNFWIVLLPIVTPFVVTLN
                     TFTVPFFRKMYWSGIAYTRRKVLGYTGDFSWRTK"
     gene            3066..4568
                     /locus_tag="PLF77_08675"
     CDS             3066..4568
                     /locus_tag="PLF77_08675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPC08965.1"
                     /translation="MSKKQLIELVSSLQAQLASADASSLSGTSGEDLYRFLAQSSQVG
                     FYIMQDGRFRFINSHFRDYAGYTEEEMLKMDPISLILPEDRRKTAKSAVMMLKEQRFS
                     PYEFRIVTRDGRIKWIMETVMSIKFKGRRAILGNSMDVTERKEAGRRLEELEALESSI
                     LDAIPQAVVGLHERRINFANHAVKIIFGWHREELIGKSIRMIYRNEEEADTIGDLFYS
                     TLEKQRTYETEFPCQCKDGREIICRMKASRIGEKLREKRIVVTFEDITAQKKAQEDLE
                     RSRQDLRNLSAHLQSVREEESTRMARKIHDELGQSLTALQMDLSWLKTRLPTSNNSIR
                     EKTKSMSELVDATIESVHTITTELRPSLLDDLGLPAAIEWQAGDFQKRSGIRCQAYIT
                     GNMETISKDLATSIFRIFQETLTNIARHSKATQCKVTLSERGKELCLEVTDNGIGITQ
                     WQIDDSRSFGIIGMRERAHLWDGTVHVRNAKPSGTTVRVLIPLEKGEKLS"
     gene            4565..5197
                     /locus_tag="PLF77_08680"
     CDS             4565..5197
                     /locus_tag="PLF77_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPC08966.1"
                     /translation="MIRILVADDHAVVRQGVKQILADVTDMTVQDEAQNGPETLRKVT
                     DNNYDVVLLDISMPGRSGLEVLEDIKAQHPKLAVLILSMHPEEQYAVRALRAGASGYL
                     TKASAPQELIGAIRKVAGGGKYVTSSLAEKLADELEIDTEKLPHERLSNREHQVMLML
                     AEGKSVSDIADELCLSVKTISTYRTRVMSKMGMKKNAELTLYAVHNKLIG"
     gene            5406..8087
                     /locus_tag="PLF77_08685"
     CDS             5406..8087
                     /locus_tag="PLF77_08685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC08967.1"
                     /translation="MNQEFSTSEKAQSTKKNVQSDSSFARALLKDELAETIHQAVRDN
                     RSYLMEHEAKEILEGIGIRTTGFLVARSEDEVLAMSEKIGYPVVLKIVSPDVVHKTDA
                     GGVKLNLHSAGDVRTAYQDILEKFKYQHIEGVTVQKMAKPGIEAIIGVTRDPSFGPLI
                     MFGLGGVFVEVLRDVSFRILPITEKDASEMIAEIRGAAILEGYRGQAVDLDSLSNLLM
                     KISQLVIENPEISELDLNPLFLYPDGYITVDARMFVKETSPGENVTATGRESLRDFFY
                     PKSIAVIGASDVKGKLGYNVFWNLINHDFPGMLYPINPKKDSIKGVKCYKSILDVPDP
                     VDMAIVIVPAKAVEAAISDCCSKGVKYVVVEAAGFAETGEEGRQAQERIEKIIRAHGC
                     RVLGPNCSGVINTHHNMVQSIGLLSDLHKGNVGMVAQAGVYAAGILTGLSNVLNFGIV
                     ATIGNKMDINETDILEYLSDDDHISVIVMYMEDIRSGKRFVDVASRAAVRKPVIILKS
                     GRTEAGKKAVSSHTASLAGNDEINSAAFKQSGIIRARDNEHLFALTRAFSKQPVPKGN
                     GVLVITYTGSMGVAATDMLYLSNMRLAALEPYFQKRLKQVMPDYASIGNPIDCSFSMT
                     PRQVKDLIDIGVESGDVHGFIVIIQGEILGSFVDVMKNIDYKEKPVLCCVACKEFMID
                     DVVKMEQAGIPVYSTAEMAAEVLSQMYHYGVRRQSAIADSIARHITRNSLSVDNRAVR
                     LRLINPKDIGLWTDFVNNCSEKSLWLRFLSPFSATPERAQRFCNVNPEEEIAVVAEMK
                     DGDKYKFLGIARLIKNKRCEGEAEYAIIVSDNWQNKSLGHTLSEQCIELARKEGYKTI
                     RAETLQENYAMLRIFRHCDFVLDSKDENMVSMTLNLV"
     gene            8306..9412
                     /locus_tag="PLF77_08690"
     CDS             8306..9412
                     /locus_tag="PLF77_08690"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPC08968.1"
                     /translation="MEERRKSPRIKDENEVTITVFSEENNIPKEKIIENFTKDISAGG
                     AKLQTNILMPVDTLIELEFTPKGLRQQINAIGKVKWVKVLIDDESYQAGVEFSGPPNE
                     AVSKLQDYISWKLKSHKVDYIKEKLSPVDSGDVNIEKKPEIAPIDPAAIEITKDDAIP
                     RDQSGQKDTEKIKREPRARDRKWIAIPIFVFFVLVLAAVLLQAFGVIPSLNNLLYPET
                     KLSKTAIKNTEILQKPAVESGVPAENLPAEVPAAVEPVSEETVIKNMLDKWINSWKSG
                     DMEAYRSCYAPDFRSKEKDLNAWITHKANIFKNSKDIAISINDVRIHLNGDSAKVLFI
                     QQYSSSLIKSSGKKILHLKKIDNGWRIHKELIRP"
BASE COUNT         2633 a         2401 c         2392 g         2249 t
ORIGIN      
        1 cccggaccgt caagcagatg gcccggctcc agcacctttt tggcataaca gatgacctca
       61 gttctcatac cgaatctcgg cgccattagc gagcgatggc cacgggctac ttccagaatg
      121 gttctcatgg tttcgaaatg aatcagatga tacgggcgtg tgaacacata aaaaggcccg
      181 gggcctgtct cagacggaaa ccagtccagc atgcgacgct ggtaggcatc gtccgtatag
      241 ccgacggcaa acacgcctcc tttgggtcgg gctcctaaaa cataatcaac cagcggcgcc
      301 tgaccgggtg tccacagatc ggaaagagga aaatggtcaa aaagttctat ggtttcattc
      361 attttcggtc cgagcatccc cggttgaaca acagagcatc cgagggcatt ggcaacgatt
      421 gacatttcca cacagagctt tgttccatcc gtaaatgaag aacacatttt atgatcaaga
      481 aatcgtttat cggcttcagg agtgattttt acgggatcgg tgtaacgatc aaggaaccct
      541 ttgatgttgc cggccatcac aaagtcgaga ccgtaaaacc gcaattcttc cactaaacgc
      601 gcaatcacgg caggttgatc accgtcgctg ctcgtatagg caacgccgta tttctgtgcc
      661 agatgccaga accaggggcc gaaaacagcg tccgcttcgg cattcatcat aacaacgtgt
      721 ttccccgaac gcatggcgga ttcgatggtc cgggaggcgg agtccacagc tgtcgaagca
      781 tcaaaaaaaa cttcaacacc gggggcaata gccgccagtt gagcatcctc gcaaacggca
      841 acctttcccg cgtttaaagc atcggcaagt tctccttcgg ttgagacgat ctcgaagcgc
      901 cgtcgaggat cctccaaagc catcaccttc tccatcgctt tatcgcaaat gactttgcaa
      961 tccacatccc gggtcagcaa agattgatgg aaaacccctc cgccaaccga accggctccc
     1021 agaagaacaa aaccgacagg ggccatcttt tcgacgcttg aatatttttt catagaagac
     1081 ctcaaaaaaa cgaaatgtct ttccccgggg aaaatatact tgaattgcgg atgcgcgtca
     1141 atggattacc aaccttactg tcgcgggatg taaatatttt tcccaatgcc ctcaaggctg
     1201 cctcatgttt gaaataaaaa cggtccctga ccaattttta aagttgcaga tagatccgga
     1261 tgcaaccatc accccggcag aaagcttcgt cactcgtgct ccgatgtggc ttttcggtac
     1321 cgtatccgaa tatcaatgac ctgttaaaat tccaatgaat attaccttga cagacaggtc
     1381 agcatcgatt atgttcccgc agtacttttt taacattagt aaaatggata tagacatcat
     1441 gcaggatgaa ggccgtctta cggcgaaaag gtttttttca tttcaccgga cgatattgca
     1501 tcactctttc tcctcgctgc aatccatgag ctgaatggat gtggacgttc tgtgattgaa
     1561 ttcattggat atttttgcgc atttctggcg attgccagct cgctgatcgg attactctct
     1621 gtttttatct tccgctcgca tatcaagcac tggaccagaa agacgccggt aacgtatccg
     1681 gcgctgacgg tgatttctcc gcaacggggc aaaatcgacc cggaaaatgt cgaggctatc
     1741 ctggaacaaa attatcccgg cgtctgggaa attatttttg tcacgacaaa agacgatgct
     1801 tccctgccgc agttacaaca ctatttacaa aaatataaaa atgtaaaaat agccatcgcc
     1861 gaagacgtgg ttgaactggc aacaacaaaa gggattcacc ggtgtcagaa gaacaataat
     1921 ttattaactg cgattaaagc cgcctctccg caaacagaag tttatgctat tattgatgcc
     1981 gatgcccgtc ccttcagtga ctggctaagc tgtctcgtcg ctcctctcac tgacgccaat
     2041 tccaggctgg gggttgttac gtccgcaaga atttaccttc cggggaaagg gctggcctct
     2101 ctggttcaag ttttatggat tttgatttct gccatgtttc ttgtgggaaa gtgccgctat
     2161 atatggggag gcggactggc cattcccaaa aaagtatttg ataatgcaaa cctggcgcga
     2221 cagatcaatg gacagggggg aagttccatc accaccgacg acatgaatat atataatgcc
     2281 ttgcggaaac agggatacga gtctctgttc gtacctgact gtatcgttct cagacatcct
     2341 ccgaagcata aggaaagttt attcaacgtc atcggattta ccaaccggca ggtcttgcag
     2401 acctggtgga cgaccagggg catatgggtt ttgctgtgca atatcggcat caggctgccg
     2461 atgcttttat gcgcgctggt catagcctgg tggtatccct ggtgtctgct ggcgttatta
     2521 tccattgtca ttgacgtctt catgggaatc tccgcgctga aaaccgtcat cgacgcagaa
     2581 ccgagattaa gcgccaaatt gattttctgg atcatgaagt caaaaggagc cctcgccggc
     2641 cgctcactgc aaccggaaat aagaatccaa gtcaatttct ggatcgttct gcttcccatt
     2701 gtcacgccgt ttgtcgtgac ccttaacacc tttaccgtac cttttttcag gaaaatgtac
     2761 tggagcggaa tcgcatatac aagacggaaa gtcttgggat ataccggcga tttttcctgg
     2821 cggaccaagt gacgccaaat cgatttttga tattgctttc aagttgtttt ccatcccccg
     2881 gttctcttca cgggttattt cgattttttt gaatcgacat cctgaaaaaa gcgtctcata
     2941 gatacagctg ccatctgttc tggaaaaacc ggccatcaaa gatatttatt tttgatgtgt
     3001 tttatgatta aataattttt tcatggctga gaatagaatc gggtggctca atgaatccgg
     3061 aaaagatgtc caaaaaacag ctgatcgaac tggtgagcag tcttcaggca caactggctt
     3121 cagccgacgc gtcgtcccta tccggcacgt ccggagaaga tctttatcga ttcctcgccc
     3181 aaagctctca ggtcggattt tacatcatgc aggacggccg ttttagattc atcaactcac
     3241 attttcgcga ctacgccggt tataccgaag aagaaatgct caagatggat ccgatttccc
     3301 tcatcctccc cgaggatcgc cggaaaacag caaaaagcgc cgtcatgatg ctcaaggaac
     3361 agcgcttttc tccttatgaa ttccgtatcg tcaccagaga cggccggatc aagtggatca
     3421 tggaaacggt catgtccatt aaattcaagg gaagacgggc cattctggga aattccatgg
     3481 atgtcaccga acggaaagaa gccggtcggc gcctggaaga gctggaagcg ctggaatcgt
     3541 ccatcctgga tgccattccc caggcggtcg tgggacttca tgaacgccgg atcaattttg
     3601 ccaatcacgc ggtaaaaatc atttttggct ggcaccggga ggaactcatc ggcaaaagca
     3661 tccggatgat ttaccgtaac gaagaagaag ccgacacaat cggcgacctg ttttattcca
     3721 cgctggaaaa acaacgcaca tatgaaaccg aattcccctg ccagtgcaag gacggccggg
     3781 aaattatctg ccggatgaaa gcctcgcgga tcggtgaaaa actgcgcgaa aaacgtatcg
     3841 tggtgacctt tgaagacatt accgcacaaa agaaagctca ggaagatctg gaacgctcac
     3901 ggcaggactt gagaaatctt tccgcgcatc ttcaatcggt gcgtgaagaa gaaagcacgc
     3961 gcatggcgcg caaaattcat gatgaactgg ggcagtcgct gacggcgtta cagatggatt
     4021 tatcgtggtt gaaaacccgt ttgccgacca gcaacaatag tatccgggag aaaacaaaga
     4081 gcatgtcgga actcgttgac gctaccattg aaagtgtgca cacgatcacc acggaactgc
     4141 gcccgagtct gctcgatgat ctgggactgc cggcagcgat cgaatggcag gctggcgatt
     4201 ttcagaaacg atccgggatt cgctgtcagg cgtatatcac cggcaatatg gagaccatca
     4261 gcaaggatct ggcaacctcc atctttcgta tttttcagga aacactcacc aatatcgccc
     4321 gtcattccaa ggcgacacaa tgcaaagtca ccctttcgga gagaggaaag gaattgtgtc
     4381 tggaagttac ggacaacggg atcggcatta cgcaatggca aattgacgat tcccggtctt
     4441 tcggcatcat cggcatgcgt gaacgcgctc atctctggga cggcaccgtt catgtccgca
     4501 acgccaaacc gtccggaaca acggtaaggg ttttaattcc attggaaaaa ggagaaaaat
     4561 tgtcatgata cgtatactgg tcgcggatga tcacgccgtg gtgcgtcagg gtgtcaaaca
     4621 aattctggcg gacgtcacgg acatgaccgt tcaagacgag gctcaaaacg gtcctgaaac
     4681 cctgagaaaa gtcaccgaca acaactacga cgtggttctg ctggatattt ccatgccggg
     4741 ccgcagcgga ctggaagtgc tggaagatat taaagcccag catcccaaac tggccgtttt
     4801 gattctaagc atgcatccgg aggaacaata cgcggttcgg gctctgcggg ccggagcctc
     4861 gggctacctg accaaggcca gcgccccgca ggaattgatc ggcgccatcc gcaaggtggc
     4921 gggaggcgga aaatatgtca cgtcctcgct ggccgaaaaa ctggccgacg agctggaaat
     4981 cgacacggaa aaattgccac atgaacgtct gtccaaccgc gagcaccagg ttatgctcat
     5041 gctggcggaa ggaaagtccg tttccgatat cgccgacgaa ttatgcctga gtgttaaaac
     5101 tatcagtacc taccggacgc gcgtcatgtc caaaatgggc atgaaaaaaa acgcggagct
     5161 caccctgtat gcggttcaca acaaattaat cggttaggtg tttacgtttc tgcttttttc
     5221 catgcccgcg ctgcacgttt gtcggacaaa atccgtcagg cgattgcgca ctgttccgac
     5281 aaacaaacaa gctttccgcc gatattacct tttcaatctt catgctaatc cgttaccatg
     5341 ttcaagtttg gcattaacgt gtaccgtaaa gagaaaacgt cggataattg cagggaggcg
     5401 ggggcatgaa tcaggaattt tcgacatcag aaaaagcgca atcaacaaaa aaaaatgttc
     5461 agagtgattc atctttcgca agagcgcttc tgaaagatga actggctgaa accattcatc
     5521 aggccgtcag ggataaccgc agttatctga tggaacatga agccaaggaa atcctcgaag
     5581 gcatcggcat ccggaccacc ggctttctgg tggcgcgttc cgaagatgaa gtgctggcca
     5641 tgagcgagaa gataggctac cccgttgttc tgaaaattgt ttcacccgat gtagttcaca
     5701 aaaccgacgc gggcggcgtc aaactgaacc tgcattccgc gggagatgtg cgcacagcgt
     5761 atcaggacat tcttgaaaaa ttcaagtatc agcatatcga aggcgttacg gttcagaaaa
     5821 tggccaagcc gggcatcgaa gccatcatcg gcgtgacgcg cgatcccagc ttcggtccgc
     5881 tgatcatgtt cggcctcggc ggcgtatttg tagaagtgct gcgcgatgta tctttccgga
     5941 ttctgccgat cacggaaaaa gatgcttccg aaatgatcgc ggaaattcgg ggggccgcca
     6001 ttctggaagg ctatcgcggc caggcagtcg atctggactc cctcagcaac ctgctgatga
     6061 aaatatcgca actggtcatc gaaaatccgg agatcagcga actggacctt aatccgctgt
     6121 ttttatatcc tgacggctat attaccgtcg acgcccgaat gtttgtcaag gaaacatccc
     6181 ccggggaaaa tgttaccgcg acaggccggg aaagcctgcg ggatttcttt tatccgaaga
     6241 gcatcgccgt gatcggcgca tccgacgtca aagggaaact gggctataac gtgttctgga
     6301 atctcatcaa tcatgatttc cccggtatgc tttatccgat caatccgaaa aaagattcca
     6361 tcaagggcgt gaaatgctac aaatcgattc tggatgtgcc cgatccggtg gacatggcca
     6421 tcgtcattgt tccggccaag gcggtggagg cggccatttc cgattgctgc tcaaagggtg
     6481 taaagtacgt cgtggtggag gcggcaggat tcgccgaaac cggcgaggag ggaagacagg
     6541 cgcaggagag aatcgaaaaa atcatccgcg cccacggatg ccgggttctg ggaccgaact
     6601 gttccggcgt catcaacacg catcacaaca tggtgcagtc catcgggctg ctttccgatt
     6661 tgcacaaagg aaatgtcggg atggttgccc aggccggcgt ttacgcggcc ggaattctga
     6721 caggattgag caacgtactg aatttcggca ttgtcgcgac catcggcaac aagatggata
     6781 tcaacgaaac agatattctg gaatatctga gtgatgatga tcacatttcc gtgattgtca
     6841 tgtacatgga agacatccgc agcggaaaac gttttgtcga tgtggcttca cgggcggccg
     6901 tgcgcaagcc ggtgatcatc ttgaaatcgg gacggacgga agccggtaaa aaagccgtgt
     6961 cttcccatac agcctcgctc gccggcaacg acgagatcaa cagcgccgcg ttcaagcaga
     7021 gcggcatcat tcgtgcccgc gataacgagc atttattcgc actgacccgc gccttctcca
     7081 aacagccggt accgaagggg aacggcgtcc tcgttattac ttataccggt tcgatgggcg
     7141 ttgccgcgac ggacatgctg tacctttcca acatgcgtct ggccgctctg gaaccgtatt
     7201 ttcagaaacg tctcaagcag gtcatgcccg attatgccag tatcggcaat cccattgact
     7261 gttcgttttc catgacgccc cggcaggtga aggacctgat cgacatcggc gtggaaagcg
     7321 gcgatgtcca cggttttatc gtgatcattc agggggagat actcggctct tttgtggatg
     7381 tcatgaaaaa catcgattac aaggagaagc cggttctctg ctgcgtggcc tgcaaggaat
     7441 tcatgatcga tgatgtggtc aagatggagc aggccggtat tcccgtttat tccacagcgg
     7501 aaatggctgc ggaggtgctc agtcagatgt accattacgg tgtcagacgc cagagtgcaa
     7561 ttgccgattc catcgcccgt cacattacca gaaattcact ttccgtggac aaccgcgcgg
     7621 taaggctgcg cctgatcaac cccaaggaca tcggcctctg gaccgatttt gtcaacaatt
     7681 gttcggaaaa gtccctgtgg ctgagattcc tgtcgccctt ttccgccaca cccgaacgcg
     7741 ctcagcgttt ctgcaatgtg aatcccgaag aggaaatcgc cgtcgtcgcg gagatgaagg
     7801 acggcgacaa gtacaaattt ctcggcattg cgcgtctgat caaaaacaag cgctgtgaag
     7861 gcgaagcgga atatgccatc atcgtctccg acaactggca aaacaaatcc ctggggcata
     7921 ctctctccga gcagtgcatt gaattggccc ggaaagaagg gtacaaaacc atccgtgcgg
     7981 aaacgcttca ggaaaattac gccatgctga gaattttccg ccattgtgat tttgtactcg
     8041 attccaaaga cgaaaatatg gtttccatga cgttaaattt agtctaaatt caccccatag
     8101 tttgcagctc atacacataa cggaacggga acaatgacct ttaccgtgcc gccccgatgc
     8161 tgttttcctc ccccatatcc ccttaggcat gaaacattgt gccgtcaatc ttttattgac
     8221 agacaaaaat gatttcaact atattgagcc ataggtcatc agattcggga tagaagtaaa
     8281 atccggcatt caaaggagcg aatgaatgga agaaagacga aaatcgccca ggatcaaaga
     8341 cgaaaacgag gtcaccataa ccgttttttc cgaagagaat aatattccga aagaaaaaat
     8401 cattgaaaat ttcaccaagg acatctcagc gggcggcgcc aagcttcaga ccaatattct
     8461 gatgccggta gatacgctta tcgaactgga gtttacgccc aaagggttgc ggcagcagat
     8521 caatgccatc ggaaaagtca aatgggtgaa ggtgctgatt gacgatgagt cgtatcaagc
     8581 cggtgtggaa ttttcaggcc cgcccaatga agccgtcagc aaacttcagg attacatctc
     8641 atggaaatta aaatcgcaca aggtggacta cattaaagaa aaactttctc ctgttgattc
     8701 cggcgatgtt aatatcgaaa aaaaaccgga gattgccccg atcgatcccg ctgcgataga
     8761 aatcacaaaa gacgatgcaa ttccccggga tcagtccgga cagaaagaca ctgaaaaaat
     8821 caaacgagaa cctcgcgcaa gggatagaaa atggatcgca atacccatct tcgttttttt
     8881 tgtccttgtg ctggccgcgg tcctgcttca ggcgtttggc gtcatcccgt ctttgaataa
     8941 tctgctctat cctgagacaa aactatccaa gacggctata aaaaataccg agatactaca
     9001 gaaaccagcg gtagaaagcg gcgtgccggc ggaaaatttg cccgcagaag tccctgcggc
     9061 ggtggaacct gtctcggagg aaaccgtcat aaaaaatatg ctcgataaat ggatcaacag
     9121 ctggaaatca ggcgatatgg aagcctaccg cagctgttat gcgccggatt tccggtcgaa
     9181 agagaaggat ttgaatgcct ggattactca taaagccaat atattcaaaa acagtaaaga
     9241 tattgccatc agcataaatg atgtaagaat ccatttaaac ggcgacagcg cgaaagttct
     9301 ctttatccag caatacagtt cttctttgat aaaaagttcc ggcaaaaaaa tattgcattt
     9361 aaaaaagata gacaacggtt ggagaatcca caaggaattg atacggccgt aaggaaggca
     9421 tccccgccgg aagttcgaaa tcaagaaaat gccgtttccc gatcttttgg gaaacggcat
     9481 ttttaggaag gaaattctgc gcgaaaaaga cagaaatttt tcgcgcagat aaaaatttta
     9541 accgagtttc tttctattgt tcaccaaatc atccacgact gccggatcgg ccagggttga
     9601 cgtatccccg aaatcaccga atgtcttggc ggcgacgttt ctcaaaacac ggcgcataat
     9661 cttgccggat ctcgt
//
LOCUS       DAOOLB010000074         9586 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_8288_length_9586_cov_54.793201, whole genome shotgun sequence.
ACCESSION   DAOOLB010000074 DAOOLB010000000
VERSION     DAOOLB010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8288_length_9586_cov_54.793201"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..2192)
                     /locus_tag="PLF77_08695"
     CDS             complement(276..2192)
                     /locus_tag="PLF77_08695"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015204 - urea transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071918 - urea transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HPC08969.1"
                     /translation="MLLNLRDKLQGVLEEAAGWIKAPDSGPNALQAAVIVLRSYGQVV
                     FAAHAVSGLFLLIALATISRSTVWFSLVGIAAASLSAYAFRAERSFIRQGIFGFNGAL
                     LGIFWSWYFTVSIVSVLLLVVMAVIVYLVQSFLMNKLSLGRFNLPVMSLPSVIVLIAS
                     LLIVYWLVYNAWLIPASAIYRMGGYAAEPLIPMEGVLKSLLPGLPLPRHVVAWLLIFT
                     GILIFSRISAFAAALGALSGYAITRMLPATTGVLYTSDLFIGFNAIPVSIGLLGVFLV
                     ANGPAFFFTFAGLVVCSVFWLLLIQLFGLLNFPFLTLPFNLTILAVLITLKKMNSPPA
                     GLYPVPLEVITTPEQIILWHKNNILPRAAVKTPETVAQLLLHPSRLFRPSREEINAVQ
                     VLVDGAEKIAVLSGAGTSTESGIPDFRGHYNFWRKFGAEDFTYQNFLNREDVRARYWA
                     MERQFYAMILRARVNPTHQAAKWLDDQNRLSCIVTQNVDGLFQKAGVNPEKVIEIHGT
                     EHQVRCLNCGAIGNRQDIETMFPAGIYIPYCYACYGWLKPATVLMGEELDKVLFESAL
                     SKILSSDLLIIMGTSLQVDPVASIPALAWKKGIPIIIINSTPTPQNHLSKLFIHCPTG
                     KFFRKFLRQVEKRR"
     gene            complement(2170..3549)
                     /locus_tag="PLF77_08700"
     CDS             complement(2170..3549)
                     /locus_tag="PLF77_08700"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPC08970.1"
                     /translation="MIKNKGTKPYVRPVIKKISFGDTVKFKNEHMKTPAVKALEGLSV
                     SDLTDRFGSPLYVVSEGTLRRSFREVAQAFTRRYPRTIIAYSYKTNYLSGICALFHSE
                     GAWAEVVSGFEYEIARRLGMDGPSILFNGPYKKPEEMKRAFLEGASVHLDSFDELKMA
                     GEVAASIGRKVNVGVRVNMQLNYPAWDKFGFSYERGEAFEACRQIKKHRYLQLSGLHC
                     HAGTYIIDLGIYRRVIENLTDLSLDIQKELGREVEYLDIGGGYPSGNSLHTQLMPGYT
                     IVPPMEQYAETVCSVLNGKLRSFTKAPRLILEPGRSVVDEGMHLLTKVVSVKERQNGN
                     RAVIVDAGIHLLPVSAYYRYEMSSNRDSTPTTEDVDVYGALCMQIDVLRKSVRLPVAQ
                     AGDILTIHKVGAYNFTQSMQFIYPRPAVVLLHDGRAEILRRRETYEDIKGPEAIPARL
                     LTYAAKPKR"
     gene            complement(3536..4480)
                     /locus_tag="PLF77_08705"
     CDS             complement(3536..4480)
                     /locus_tag="PLF77_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08971.1"
                     /translation="MIIAVTGLNTAAEPEAGFGVLQSLSGGGAGHDRLIGFAYQPLDA
                     GLFHSGLMHRAYVVPPPKTDRMAFLKRITLVKKKRGLDVLIPALDDEMELFVDMAPQL
                     RAAGIAVLLPSGKSLRELQSEMYRQIGKQQAAAGNDEQDDPPVLPPALVKNPVSEQTA
                     MDAAVPDYFSVAVVANSQSRIVAIASVRKLLISRYGGTWMALTVDNHEFLGVAEEVVT
                     STAWQGALTIDVARNRQGEQYLSGFHPTFPDWIGLARGAGANLPAVLIDLIKKKRIRG
                     VAQTAPGKVWIRSSIDLVTDLNHFGDISLNGEMTYDQK"
     gene            complement(4477..5526)
                     /locus_tag="PLF77_08710"
     CDS             complement(4477..5526)
                     /locus_tag="PLF77_08710"
                     /inference="COORDINATES: protein motif:HMM:NF026968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPC08972.1"
                     /translation="MLQAHVVITGLMATDNPYPGLGIARCLRDAPEFRGRISGFVFDP
                     LSTGAFCEGVFERVFLVPYPAAGVEVLLERIGEIHRADPIDVLIPSLDSEVILYAQAA
                     RELATLGIRTLLPPVNSIKMRSKNQLYEFCRQHRFRCPETVILNNVQEIAARCEALGG
                     TPFVLKGALSDAAVCHSIEEGEYQYGQLFNQWGYPILMQQYIAGEEIDVIALADKKSR
                     LSGAVVMKKFGLTPKGKAFSGITVEDDKPVQLTRQILKSLKWIGPAECEFLKDEKGHY
                     FLMEINSRFPSWLYLAAAAGQNLPLLTVQLACDMPVRPLTSYTAGKLFVRTVADALLD
                     ARQIMELTASGEVRL"
     gene            complement(5528..5935)
                     /locus_tag="PLF77_08715"
     CDS             complement(5528..5935)
                     /locus_tag="PLF77_08715"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HPC08973.1"
                     /translation="MKSLRKSQGKTIRCVLAGGQTKSCPPVKDEGLPLCEKHKRELNR
                     SARCRIADDIRGNFMEKGDESIVFNPKDGGVYSLNGTGTFIFKEIMAGKTFCEILAKS
                     QSMFDAENVSEMIADYRLFIEELKKSNVITAEA"
     gene            complement(5937..8777)
                     /locus_tag="PLF77_08720"
     CDS             complement(5937..8777)
                     /locus_tag="PLF77_08720"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC08974.1"
                     /translation="MKSKCLRISLFAALFAAAFVFAASIPVRAAWFSKGGKDYYQEGL
                     NHFQKKNYYLSMQSLEKALQLKPDYPEALGVLGWDYIKVGRALDAEAIFTKKYEKNNA
                     DISAIQGLAWAKFALGKNEESEKYFNMELKWANDFITKDNWIFFGPSDKEYIESIYSD
                     ANYGLASLAGAQKNYALAAKYFETALQYPNSFTSRTDLLTACGDIYYYQAKYAEAIPV
                     YERAVKEDQKNLSAQLKAAWSYYFNNQFAAAGAAFEKALAVSSRQESVEALYGMALTD
                     YRQNKFDQAYRNLARAIAVNPYYMDNAVVHDMIAKKPEWKTLWKNFGLAYTASNYYAA
                     VYKLDGYLQVVKADDVEALLADGWSYLGLGYLDKALQLFEAALKFNPKSDEAYVGLGS
                     SYLAYKKPAEALKAYQQALAINPQSALACNGLAYYYLSQKDEAKALESAQKSVSLKSD
                     YYDSQAFVGNALFRQKKYDQAIKEYDKLIKIDPSVFSSWNLLGWANYSAGKYDAAVKA
                     FAESKRINPYLVEAHYGLGMCYARTGDMNDAKDEFSAAINLYPYYSHTKDLVSLIKAN
                     PKWNDLYKTLGWSYYNYQQYSLAASAFKEYLAAEPGDIGALRGMAWSNYWLGQLDTAY
                     AGFRGILKEEANDVDALVGTGWILYIRGKDGEAMGYLQKAVKINDKALNAWRTVAAIH
                     FRAKRFEEASAIYKKIADLQPYAVDTYNNQGWALYKENKFKEAVAKFNESLRVNRYLG
                     EPYYGLGLSYAKLGDIEKARENFASAVYLYPAYMDGQDLYAVFDSNPKLKDLYSALGW
                     GYYYQYYYDKAKFHFNKALKADAGNSDALLGVGTVAFILGDFNGAIEAYKKLLPNVPA
                     KASFWDKYSYMLDNLGWSYYYQKQYDSALETFKRLEAYHPGISYIAPINGKGWCELKK
                     GNKDEAQKLFQQSLKIVPYNYTAEAGMKEMKK"
     gene            complement(8833..9360)
                     /locus_tag="PLF77_08725"
     CDS             complement(8833..9360)
                     /locus_tag="PLF77_08725"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPC08975.1"
                     /translation="MGNVKIALRPSSISDASLRYHWFNDAEFTRLYLGRPLFTSYRQV
                     EDEILLSTYPTLSTGLYELAIQTVETNLYIGNTFFRKMDWQNRSAEYGIFIGDSERRG
                     AGIGPEVTQRMLEQGFSEFGLHRIWLTVLAFNERAIRCFEKCGFQREGVLRDAIFSGG
                     QFNDVMMMSVIAGAN"
BASE COUNT         2222 a         2610 c         2542 g         2212 t
ORIGIN      
        1 cagaagaaac gctcgatcac cagatgaaaa tgaaaaaagc ctgggagcag gaaaggaaac
       61 caactccctc gcaggctgca tcctgatcaa agcgccgggc agacgcgctc gcgcgcggcc
      121 cggcgttatc ccacccttgt ccgtacctgg agaaaacaca ttccaaacag cgagcaacct
      181 cgcggctggt cccttccgga cagtgggtgg tcagatatcc ataaagagca tgcttcgttt
      241 ataaagactc ccccctgctg cttgagggtt tcaaatcatc gtctcttttc gacctgccga
      301 agaaatttcc tgaagaattt ccccgtcggg caatggatga acagcttgga cagatgattc
      361 tggggggtcg gagtggaatt gatgataata atgggaatgc ccttcttcca ggccagggcc
      421 ggaatggaag ccacgggatc cacctgaaga ctggtcccca taatgatcag caaatcggag
      481 gaaagaattt tggacagagc gctttcgaac aggactttgt ccagctcttc acccatgagc
      541 accgttgccg gcttcagcca accgtagcag gcgtagcaat aaggaatgta aattccggcg
      601 gggaacattg tttcaatatc ctgccggttt ccaatggcgc cgcaattcaa acaccggact
      661 tgatgctccg tgccgtgaat ttcaatgacc ttttccgggt tcacgccggc tttttggaaa
      721 agtccgtcca cattttgcgt aacaatgcag gaaaggcggt tttgatcgtc cagccatttc
      781 gcggcctggt gggtgggatt cacccgggcg cgcagaatca tggcataaaa ctgccgctcc
      841 atagcccagt agcgcgcccg gacgtcttcc cggttcagaa agttttgata ggtaaaatct
      901 tccgcgccga attttctcca gaaattataa tgaccgcgaa aatccggaat gccggattcc
      961 gtgctggttc ctgcaccgga cagaacggca atcttctccg caccgtccac aaggacctgg
     1021 accgcattga tttcttcacg gctcggcctg aataaccggg agggatgcaa aagcagctgc
     1081 gcaaccgttt ccggcgtttt cacggcagcg cggggcaata tattattttt atgccagagg
     1141 atgatttgtt ccggtgtggt gatcacctcc aggggaaccg gatacagtcc ggccggcggt
     1201 gaattcattt ttttcagcgt tatcagaacc gccagaatgg tcaaattaaa cggcagcgtc
     1261 aggaaaggaa aattcagaag accgaaaagc tgaatcagca ggagccagaa aacggagcag
     1321 acgacaaggc ccgcaaaggt gaagaaaaaa gccggtccgt tggcgaccag gaaaacaccc
     1381 agcagaccga tggacacggg aatggcgttg aatccgataa acaggtcgga tgtgtacaat
     1441 accccggttg tggcgggcag catccgggta atggcatagc cgctcagcgc gcccagcgcg
     1501 gccgcaaatg cgctgatgcg cgagaaaatc aaaatgccgg tgaaaatcaa cagccaggca
     1561 acgacgtgcc gcggcagcgg cagcccgggc aggagactct tcaaaacgcc ttccatgggg
     1621 atcaggggct cggcagcgta tccccccatc ctgtaaatgg cggatgccgg aatcagccaa
     1681 gcgttgtaca ccagccagta aacgatcaac agcgaagcaa tcaacacgat cacagagggc
     1741 aggctcatga ccggcaggtt aaaccggccc agcgacagct tgttcatcag gaaagattgc
     1801 accaggtaaa caatgacggc catcacgacc agcagcagga cggacacgat ggaaacagta
     1861 aagtaccagc tccagaaaat gccgagcaac gcgccattga agccgaagat gccctgccgg
     1921 ataaaactcc tttcggccct gaaggcatac gcggacaaag acgcggcggc aatgccgacg
     1981 agcgaaaacc ataccgtgct gcgggaaatg gtcgcgagcg cgatcaggag aaaaagtccg
     2041 gaaaccgcat gggcggcgaa gacgacttgg ccgtaagagc gcagaacaat caccgccgct
     2101 tgaagcgcat tcggtccgct gtccggggcc ttgatccagc ctgccgcttc ctccaagaca
     2161 ccctgaagtt tatctcttag gtttagcagc ataggtcagc agtctggcgg gaattgcctc
     2221 cggccccttg atgtcttcat aggtttcccg gcgacgaaga atttcggccc ggccgtcatg
     2281 gagcagcaca accgcaggac gggggtagat aaactgcatg gattgcgtaa aattgtaggc
     2341 gcccactttg tggatggtga ggatgtcgcc ggcctgcgcc accggcaggc gcaccgactt
     2401 tctgaggacg tcaatttgca tgcatagggc gccatagaca tccacgtctt ccgtcgttgg
     2461 ggtggagtcc cggttggagg acatttcata gcgataatac gcggaaaccg gcaggagatg
     2521 aatgcccgcg tccacaatga ccgcccggtt gccgttttgc ctttccttga cggaaaccac
     2581 tttcgtcagc aggtgcatgc cttcatcgac aacggagcgg ccgggctcca gaatcagccg
     2641 aggggccttt gtaaaggagc gcagctttcc gttgagaacg gaacagacgg tttcggcgta
     2701 ctgctccatc ggcggaacga tggtgtagcc gggcatgagc tgcgtgtgca ggctgttgcc
     2761 ggaagggtat ccgccgccga tgtccagata ctccacttct cttcccagtt ctttttgaat
     2821 atcgagcgac aggtcggtca ggttttcaat gacgcggcgg tagatgccga ggtcgatgat
     2881 ataggttccc gcgtggcaat gcaggccgga cagttgcaga taccggtgct ttttgatctg
     2941 ccggcaagct tcgaaagcct ctccccgttc gtagctgaat ccgaatttat cccacgccgg
     3001 ataattcagc tgcatgttga cgcgaacgcc gacgttgacc ttgcggccga tggaagccgc
     3061 cacttccccg gccattttca gttcatcaaa ggaatccagg tgaaccgacg cgccttccag
     3121 aaaggctctt ttcatttcct ccggcttctt gtacgggccg ttaaagagga tgctcggtcc
     3181 gtccatgccc agcctccggg cgatctcata ttcaaacccg gaaaccacct cggcccacgc
     3241 gccttccgaa tgaaacaggg cgcagattcc gctcagataa ttcgtcttgt aggagtaggc
     3301 aatgatcgtc ctcggatagc gccgggtaaa agcctgcgcc acttcccgaa aactccggcg
     3361 gagcgtgcct tcggacacga catacagggg cgatccgaag cggtcggtca gatcgctgac
     3421 ggacaagccc tccagcgctt taacggctgg cgttttcata tgctcgtttt taaacttcac
     3481 ggtatcgccg aaagaaatct ttttgatgac cggccggaca taaggcttcg tgcccttatt
     3541 tttgatcata ggtcatttct ccgttgaggg aaatatcccc aaaatgattc aagtcggtaa
     3601 ccaggtcgat ggaagatcga atccatacct tgccgggtgc ggtctgggcg acgcccctga
     3661 ttcttttctt tttgatcagg tcgatcagaa cggccggcaa gttcgccccg gccccccgcg
     3721 caaggccaat ccagtcggga aaggtcggat ggaaaccgga gaggtattgc tctccctgcc
     3781 gatttcgcgc gacatcgatg gtcaaggccc cctgccaggc cgtgctcgtc accacttctt
     3841 cggcaacgcc gagaaattca tggttgtcca ccgtcaatgc catccaggtg ccgccgtagc
     3901 gggagatcag cagtttcctg acggaggcaa ttgcgacaat cctgctttgg ctgttggcga
     3961 cgacggcaac cgagaaatag tccggcacgg cagcgtccat tgcggtctgt tcggaaacag
     4021 gattcttgac cagggccgga ggcagaacgg gcgggtcatc ttgctcgtcg ttcccggcgg
     4081 ctgcctgctg ctttccgatc tgtcggtaca tctcgctttg cagctcccgt agcgatttcc
     4141 cggaaggaag cagcacggca atgcccgctg cgcgcaattg cggcgccata tcgacaaaaa
     4201 gctccatctc atcatcgagg gcgggaatca gcacatccag gcctcttttc tttttaacga
     4261 gcgtgattct tttcaaaaat gccatccggt ccgttttggg cggcggcacg acatacgccc
     4321 ggtgcatcag gcccgaatga aacaatccgg cgtccagcgg ctgataggcg aatccgatca
     4381 gccggtcatg gcctgcgccg ccaccggaca aggattgtag aacgccgaag ccggcttcgg
     4441 gttcggcggc cgtgttcaaa cctgtcacgg caatgatcac aggcgcacct ccccggaagc
     4501 ggtcagttcc atgatctgcc gcgcgtccag cagcgcgtcg gcgaccgtgc ggacaaacaa
     4561 tttgcctgcc gtgtaggaag tcagcggccg cacgggcatg tcgcaggcga gttgcacggt
     4621 cagcagcgga agattctggc cggcggcggc ggcaagataa agccaggagg gaaagcggct
     4681 attgatttcc atcaggaaat aatggccttt ctcgtctttc agaaattcac actctgcggg
     4741 gccgatccat ttcaggcttt tcagaatctg acgggtcaat tgaacgggct tgtcgtcttc
     4801 gacggtaatg ccggaaaaag ccttgccctt gggcgtcaat ccgaatttct tcatgaccac
     4861 ggcgccggac agccgcgatt ttttgtccgc cagcgcaatg acgtcgattt cttctccggc
     4921 gatgtactgc tgcattaaaa tcgggtagcc ccactggttg aagagctgcc cgtactggta
     4981 ttcaccctcc tcgatcgaat ggcacaccgc ggcgtcgctt aaagcgccct tcagcacaaa
     5041 cggcgtccct cccagggcct cgcaccgcgc ggcgatttcc tggacgttgt tgagaatgac
     5101 ggtttccggg caccggaacc gatgctgccg gcaaaattcg taaagctggt ttttgcttct
     5161 catcttgatg ctgttgaccg gcggcagaag cgttcgaatg cccagcgtcg caagctcccg
     5221 ggcggcctgg gcatagagaa tcacttcgga atccagcgac ggaatcagga catcaatagg
     5281 atccgcacga tggatttcac caatgcgctc cagaagaacc tccacgccgg ccgcgggata
     5341 ggggactaaa aaaacgcgtt caaacacgcc ttcacagaaa gcgcccgtcg aaaggggatc
     5401 gaagacaaat ccggaaattc tgccgcggaa ctcgggcgcg tcgcgcagac agcgggcgat
     5461 tcctaagccg ggataaggat tatcggtagc catgaggccg gtgatgacga catgagcctg
     5521 gagcatgtta agcttccgct gtgataacgt tcgatttctt cagctcttcg atgaacagac
     5581 ggtaatccgc gatcatttca gaaacatttt ccgcatcaaa catgctttgg gatttggcaa
     5641 ggatttcaca gaacgtcttg ccggccatga tttccttgaa gatgaaggtg ccggtgccgt
     5701 tcagggaata cacccctccg tctttcggat tgaagacgat ggactcgtcg cccttttcca
     5761 taaaattgcc gcgaatgtca tcggcaatgc ggcagcgggc cgaccggttc aactctcgtt
     5821 tgtgtttttc gcacaggggg agtccctcat ccttcacggg cgggcagctt ttcgtctgcc
     5881 cgcccgccaa cacgcagcgg atcgttttcc cttgcgactt cctcagagat ttcatcctat
     5941 tttttcattt ctttcatgcc cgcttccgcg gtgtagttgt agggcacaat cttcaacgac
     6001 tgctggaaca gtttctgggc ttcgtccttg ttgccttttt tcagctcgca ccagcccttt
     6061 ccgttgatgg gagcgatgta agaaatgccc ggatgataag cttccagacg cttaaaagtc
     6121 tccagggcgc tgtcgtattg cttctgataa tagtagctcc agcccagatt atccagcata
     6181 tagctgtatt tgtcccagaa agacgctttt gcgggaacgt tcggcagcag ctttttatag
     6241 gcctcgatgg cgccgttgaa atcgcccagg ataaaggcca ccgtaccaac gcccagaagc
     6301 gcatccgaat tgccggcatc cgccttcaac gctttattga aatggaattt tgccttgtca
     6361 taataatact ggtaatagta gccccagccc agggcgctgt aaagatcctt aagcttggga
     6421 ttggaatcga aaacagcata cagatcctgg ccgtccatat aggccggata cagatacacc
     6481 gcagaagcaa aattttctct ggccttttcg atgtcgccca gctttgcata actgagcccc
     6541 aagccgtagt aaggctctcc caggtaccgg ttgacgcgca gggattcgtt gaacttggcc
     6601 accgcctctt tgaacttgtt ttccttgtac aacgcccagc cctggttgtt gtaagtgtcc
     6661 actgcgtagg gctgcagatc ggcgattttc ttgtaaatcg cgctggcttc ctcgaaccgt
     6721 ttggcgcgga agtgaatggc ggcaaccgtt ctccaggcgt tcagggcctt gtcgttgatc
     6781 ttcacggctt tttgcaggta gcccatcgcc tcaccgtctt tgccgcggat gtacagaatc
     6841 cagcccgtac caaccagcgc gtcaacatcg ttggcctctt ctttgaggat tcccctgaaa
     6901 ccggcataag ccgtatccag ctggcccagc cagtagttgg accaggccat tccgcgcagc
     6961 gcgccgatat cgccgggttc agcggcgagg tattctttga aggcggatgc cgcgagactg
     7021 tactgctgat agttgtagta gctccagccc aatgttttat acaaatcatt ccacttggga
     7081 ttggccttga tcagacttac caggtctttg gtgtgggaat agtagggata aaggttgatg
     7141 gccgcggaga attcgtcctt cgcgtcattc atgtcgccgg ttctggcata gcacattccc
     7201 aatccataat gcgcttcgac caggtaggga ttgattcttt tggactccgc aaaagccttt
     7261 acggcagcgt catatttgcc ggcggagtaa ttggcccatc ccagcaggtt ccaggaagaa
     7321 aacacggatg gatcgatctt gatcaatttg tcgtattcct tgatcgcctg atcgtatttc
     7381 ttttgtctga acagggcatt cccgacaaac gcctgcgaat cataataatc actcttcagg
     7441 gaaacggatt tttgcgcgga ttccagggct ttggcctcgt ctttctgaga gaggtaataa
     7501 taggccagtc cattgcaggc cagggcgctt tgggggttga tcgccagggc ctgctggtag
     7561 gctttaagcg cctccgccgg ttttttgtaa gcgagatagg atgagcccag cccgacatac
     7621 gcttcatccg attttggatt gaatttcagc gcggcttcaa aaagctgaag ggccttgtcc
     7681 aggtagccca gtccgagata actccagccg tccgcaagca gcgcttcgac atcatcggct
     7741 ttcacgacct ggagataccc gtccagtttg taaacagcgg cgtaataatt gctggcggta
     7801 tatgccagtc caaagttttt ccaaagcgtt ttccactcgg gcttcttcgc gatcatgtca
     7861 tgaacgacgg cgttgtccat gtaatagggg ttgacggcaa tcgctctggc gaggttccgg
     7921 taggcctgat caaatttatt ttgccggtaa tcggtaagcg ccattccgta aagggcttcg
     7981 acggattcct gccggctgga cacggccagg gctttttcaa aagccgcccc ggcggcggca
     8041 aattgattgt tgaagtaata ggaccaggcc gctttgagtt gcgcggaaag attcttttga
     8101 tcttctttga cggctctttc ataaacggga atcgcttccg cgtatttggc ctgatagtaa
     8161 taaatgtcgc cgcaggccgt caaaagatcg gtgcggcttg tgaacgagtt cggatactga
     8221 agcgccgttt caaaatactt cgcggcaaga gcataatttt tttgcgcccc cgctaaagac
     8281 gccaggccgt aattcgcgtc ggaataaatc gattcgatgt attctttgtc ggaaggaccg
     8341 aagaagatcc agttgtcttt cgtgatgaaa tcgttcgccc acttcaattc catattaaaa
     8401 tatttttcgc tttcttcgtt tttgccgagc gcaaattttg cccaggccag accctggatg
     8461 gctgaaatat cagcgttatt cttttcatat ttctttgtaa aaatcgcttc cgcatccagc
     8521 gcccttccta ccttgatata gtcccatccc agaacaccca gcgcttccgg gtagtcgggt
     8581 ttcaactgca gggccttctc caggctttgc atggaaagat agtaattttt cttctggaaa
     8641 tgatttaagc cttcctgata ataatccttg ccgcccttgg agaaccaggc agcccgtaca
     8701 gggatgcttg ccgcaaagac aaaagctgcc gcaaataacg ccgcaaataa actgatccgt
     8761 aaacatttag acttcataaa tcgcctcctc ttttcgcttt taaaaagttg aatatcctta
     8821 aataaataat gatcaattcg cgccggcaat gaccgacatc atcatgacgt cgttgaactg
     8881 accgccggaa aaaatggcgt cccgcagaac cccctctcgc tgaaaaccgc atttttcaaa
     8941 acatcttatt gcccgttcat tgaaagccag aaccgtcaac cagatgcggt ggagcccgaa
     9001 ttcggaaaat ccctgttcca gcatcctttg tgtaacctcc ggcccgatgc cggctccgcg
     9061 gcgctccgaa tctccgatga agattccgta ttccgcggaa cgattctgcc agtccatttt
     9121 tcggaaaaag gtgttgccga tgtagagatt ggtctcgacc gtttgaatgg ccagttcgta
     9181 caatcccgtg gacagggtcg gatacgtgga aagcaggatt tcatcctcta cctgtctgta
     9241 agaagtaaat aaggggcggc ccagataaag ccgcgtgaat tccgcgtcgt tgaaccaatg
     9301 gtaacgaagt gatgcgtcgg aaatactgga tggccttaaa gcaattttca cattgcccat
     9361 aacagactca acaattcctg taatgaccag gtgaacgctg tatttaaatg cctgatttat
     9421 tttttagcat ttgccattct aaatgtccat tgataatttt aaaactttaa aagccctcgc
     9481 tgagcgtcaa gctactgaat aaacaacggt tttccccaat atgccctccc aaatcgcgcc
     9541 ttcccgtttg agttgctgaa ggtaaacata ggtttcgttg agcacc
//
LOCUS       DAOOLB010000075         9554 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_8326_length_9554_cov_43.645331, whole genome shotgun sequence.
ACCESSION   DAOOLB010000075 DAOOLB010000000
VERSION     DAOOLB010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8326_length_9554_cov_43.645331"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..970)
                     /locus_tag="PLF77_08730"
     CDS             complement(77..970)
                     /locus_tag="PLF77_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HPC08976.1"
                     /translation="MDLLTVSNEYLDICKIRFSVSTYGEKKKLCANILKKWGNPHVNE
                     ITPSMVLKHLETRARKVSNNAWNRDRKNLLAMFNWFRKIKGINNNPVMNIERLPEEKK
                     VEYIPPAEDIDKVMMACSGQDRVMLQCYYYTFARRGEIFNWTWEDINFEKQWYRLWTK
                     KRLHGDKQVDYFPMPEDSELYKALKWQWDHRNEESPYVFTDPETGEKFTHRNRFMKGL
                     CKRAQVKPFGFKAFRKFGPSVLNDIHRVSIKKLQRLLRHQSQTTTEIYLKKIDDDLAI
                     GLRLLEKKDTPRDTPRDIVNV"
     gene            complement(945..1295)
                     /locus_tag="PLF77_08735"
     CDS             complement(945..1295)
                     /locus_tag="PLF77_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPC08977.1"
                     /translation="MSILDIDIGLNPSIMTPKQVAGYLQISPRTVYSHWEQLGGFKIG
                     RTLRFRKEILNALFCQKEKTMERGGEKRRQKVFASVSDENGSQDLGSRKMEGAGKPET
                     GENRNRHGLADCFQ"
     gene            complement(1316..1573)
                     /locus_tag="PLF77_08740"
     CDS             complement(1316..1573)
                     /locus_tag="PLF77_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08978.1"
                     /translation="MNNYIKRRKPINNAPETLSPEKRKLLHEAKLEELMKLYEKCYAE
                     GIPRCTLIDVIEKEIYEKTIEKKQWRIDRGYKLEKFVTKKK"
     gene            complement(1584..2855)
                     /locus_tag="PLF77_08745"
     CDS             complement(1584..2855)
                     /locus_tag="PLF77_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08979.1"
                     /translation="MKTQKTTIESAQLGAPGREGASKQAGACKDEQKSMVESVTHHAL
                     ITDIENIHRLTSGIDTLDVGFYVTWGDNWDELKQRFDERKANAYGKGENIIEEEGSRP
                     HIFYAGGNRNYRYHVKYPEYDGFISIHESANQSPNVYVSFASEALHWELSEKELIELV
                     TSDIEAFGGTVSQHKISRCDLYADFRIPCGLSLEFLKSYMVGKADKTSHFMDGDKLET
                     FYCGSKSSPIELRLYDKSAEIKKDGCEERWLLLWFTDDPKNVWRIEFQIRRAVLKQFR
                     INSISDLNNKKADLWKYMTGEWFSLRNRDNQNQSRRTIHPFWEKVQACVQNFGIEQGT
                     KRHYEKSKATFIKWHMQRIVNLLITCAAILKEYDPESCLMKVNSRILQSVSKEDFKEK
                     ARKKSIELGVAVNKETKQPASDLYKKLGLGK"
     gene            complement(3166..3456)
                     /locus_tag="PLF77_08750"
     CDS             complement(3166..3456)
                     /locus_tag="PLF77_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08980.1"
                     /translation="MKERENKQYSVQLDPEVVKKIDYYAKKYDIARGKLMRNLIVIGL
                     DDMNILHKAGILKAAHFTDKVVTTIKNLINSEKIYVGKDGKLKIETKKTGKL"
     gene            complement(3777..4424)
                     /locus_tag="PLF77_08755"
     CDS             complement(3777..4424)
                     /locus_tag="PLF77_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08981.1"
                     /translation="MLFVGRKSEKKKIINSLNDGKNIILGGKFGIGRTSLIKEIARIM
                     ADERKFVFVDFSQTPGKMSEKIMKEVAISRKLKQGEKMGYKSMRYRIANMKSSKRHLP
                     VIVFDNVAKITVQKKIFLRHLIMEQHFQFIAIVEYFLPEENLFEIKAQLMPTVTLILS
                     HLKKDDVKNLFSMYAKKHNINYSENYIYNLATLSGGYPLGIVEMIKKYRGIAFDE"
     gene            complement(4461..6116)
                     /locus_tag="PLF77_08760"
     CDS             complement(4461..6116)
                     /locus_tag="PLF77_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HPC08982.1"
                     /translation="MDNISPPTSISDIRGHLEILASACNRFQMVSLARKLEAAQTLLS
                     ENPPIDVAVLGQFKAGKSSFLNNLIGQNVLPVGAIPVTTAITRVQYGIREKALIRHFN
                     GAITEIPLAEIVQFTSEAENPGNSKNVAIVDIELPSMQDYQGLRLVDTPGLGSVYKYH
                     QSTSEEWLPSVGTAILAVSSDRPLSEHDLDLIRELTSHTPNIILLLTKADILSPEQQN
                     EVIAFFQQTLQRELHRILPVYLYSIKENTKEYKQRIEEDILKTICKNRDSEFFRILNY
                     KIHSLSESCLGYLKIAMQSSLQADMNRENLRAQILNEKVNEDLVREELGIIAREHQRQ
                     TRPMIETYLEQFRSPLTKKAIKKLEMDITSWRGNLWKLSRKYETWVSETLSEELRLIS
                     KNEHSHFLGTMKKAHASFSRALEAFCKFLNDNIETVLGVKMAEVDWKIDVMEPNHPDI
                     SFTKPFDIHLDLIWFLIPMFLFRKVFERHFIANVPKEATINISRLAYQWEKSVNNAIE
                     EMRLQAINYIHEELATIENLISGTKGQTDEIKKLIIQIEKASF"
     regulatory      complement(6140..6204)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            6710..7186
                     /locus_tag="PLF77_08765"
     CDS             6710..7186
                     /locus_tag="PLF77_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HPC08983.1"
                     /translation="MVKVVHGIHVHGSGLISGKSFEPGEIVYRFPGKISQSVPTYQTI
                     QIGADRHTLDFGILANINHSCDPNTVIDTENLSVLALRAIATGDELTFFYPSTEWDMA
                     LPFACQCRSPQCLQFVAGARYLSHGVLKGYFINRHILKMHSELLSGASDSCGFQPR"
     gene            7376..7552
                     /locus_tag="PLF77_08770"
     CDS             7376..7552
                     /locus_tag="PLF77_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08984.1"
                     /translation="MMKEGRLSLEEFLSAPDGFDDVRDKVRGEPDQYEAMGRTLADLI
                     LEAGGGRLPDLKRR"
     gene            7666..8532
                     /locus_tag="PLF77_08775"
     CDS             7666..8532
                     /locus_tag="PLF77_08775"
                     /inference="COORDINATES: protein motif:HMM:NF013695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HPC08985.1"
                     /translation="MIVLIGVYSGSLTLVAITMDYGLSLCLNIMSLAALGVVLRQNVF
                     KYPYGAGKLENFTAFVYGVCLIPLAMAVFTFAVKRYLNPPQTLNLWFALLYVVALIRL
                     AVFAVWITRLGKRHPRHSPLMQAYYADYRISLVYESAIFATLLMGLLISEHGGMKIAV
                     IIDVSVASIIALYYLVVACRLIIRNFRSLIDLPLGEKYQRHILRCLIEEFDAYDGLGA
                     IYTRRSGSEVMIQIEMRFAGDTPLKQIDDLRKRLEEKLRQLDDHVVFQLIPLGPETAP
                     RDPLVEEVEEKK"
     gene            8605..9339
                     /locus_tag="PLF77_08780"
     CDS             8605..9339
                     /locus_tag="PLF77_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08986.1"
                     /translation="MIDFMPFIGDGCGNTFLVLSQRYCDRHADDHIRRLWKATGVDSV
                     LILQKSLRGDIRMLVPGNDGIRGDESGEFCGNGARVAAAFYLRHLGQSPVIESRGGVL
                     FPTIVEESAVSVEFPLPEERGGIVLAGGEPHVRTARKMTPPELMRFCNGRSRRVSANR
                     VAICSSLHLLVQTFEHSINKFTGCCGTGCIAMAYLAYRERRVERRVTVEAPGGQLTVD
                     IRPSCIIMSGPVRLIGSMEQWITDQT"
BASE COUNT         2353 a         2143 c         2026 g         3032 t
ORIGIN      
        1 ggacgctggc ctctcacgcc ggaaaccggg gttcaaaccc ccgtgggact accaattatt
       61 ttaaacactt acaaaatcag acattcacaa tatccctggg tgtgtccctg ggtgtgtcct
      121 ttttttccaa aagccttaaa ccgatcgcca aatcatcatc aatttttttc aaatagattt
      181 cggttgtcgt ttgtgattga tgcctcaaca acctctgcag cttcttgatg ctgactcggt
      241 gaatgtcatt taagaccgaa ggaccaaact ttctgaatgc cttgaaaccg aagggcttga
      301 cttgggctct cttgcacaga cctttcatga atctgtttct atgggtaaat ttttcgcctg
      361 tttccggatc agtgaaaaca taaggcgatt cttcattgcg atggtcccac tgccacttta
      421 acgctttata taactctgaa tcttcaggca tggggaaata gtcaacttgt ttatctccat
      481 gcaatcgctt ttttgtccat agtcgatacc attgtttttc aaaattgata tcttcccatg
      541 tccaattgaa gatttctcct ctacgagcaa aagtgtagta gtaacactgc aacattaccc
      601 gatcttgacc gctgcaagcc atcattacct tgtctatgtc ttccgccggt ggaatgtatt
      661 caaccttttt ttcttcagga aggcgttcaa tattcattac cggattgtta ttgatgcctt
      721 ttattttcct gaaccagttg aacattgcca gaagattttt ccggtcccgg ttccaagcat
      781 tgtttgaaac cttacgcgcc ctggtctcca ggtgttttaa aaccattgat ggagtaatct
      841 cattgacgtg aggattaccc cattttttga gtatattggc gcatagcttc tttttttcgc
      901 catacgttga aactgagaac cttatcttgc aaatatcaag atattcattg gaaacagtca
      961 gcaagtccat gtcggtttct gttttctccg gtttcgggct ttccagctcc ttccattttt
     1021 cgacttccca ggtcttggct tccgttttcg tcagaaacag acgcgaaaac cttttgtcgt
     1081 cttttttcac cacccctttc catcgttttt tctttttgac aaaatagggc attgagtatc
     1141 tcctttctga accgcagggt tcttccaatc ttgaagcccc ccagttgctc ccagtgtgag
     1201 taaactgttc gcggtgaaat ctgaagatat ccagccacct gtttaggggt cattatggat
     1261 ggatttaacc ctatgtcaat atctaaaatg ctcatcagct atccgattga tattgctatt
     1321 ttttctttgt aacaaacttc tccagtttgt agccccggtc aattctccat tgcttctttt
     1381 caatggtctt ttcgtagatt tctttttcaa tcacatcgat cagcgtgcat cggggaattc
     1441 cttctgcgta acatttttca taaagcttca tcagttcttc cagctttgct tcatgcagaa
     1501 gctttcgctt ttccggactc agtgtttcag gagcattatt gatcggtttt cttcttttaa
     1561 tataattgtt catttcatcc ggcctatttt cccaagccta atttcttgta tagatcgctt
     1621 gccggttgct tggtttcttt attgaccgca acgcccaatt caattgattt ctttcgcgcc
     1681 ttttccttaa aatcttcttt gctcactgat tgaaggattc ggctgttcac cttcatcaga
     1741 cagctttccg gatcatattc ttttaatatt gccgcgcagg tgatgagaag attgacaatc
     1801 ctttgcatgt gccatttgat gaatgttgct ttactttttt cgtaatgtct tttggtgcct
     1861 tgctcaatgc cgaaattttg gacacacgcc tgaacttttt cccaaaatgg atgaattgtt
     1921 cgcctggatt gattttgatt gtcccggttt cgaagggaga accattctcc ggtcatgtat
     1981 ttccataaat cagccttctt gttgttcaga tcggaaatgg aattgatccg aaattgcttt
     2041 aagaccgctc tccggatttg aaattcgatc cgccagacat tcttcggatc atccgtaaac
     2101 cacaggagca gccatctttc ctcgcagcca tccttcttaa tttctgcgga tttgtcgtaa
     2161 aggcgaagct caataggtga acttttgctg ccgcaataaa aggtttccaa tttatcacca
     2221 tccataaaat gcgatgtctt atccgccttg ccgaccatgt aacttttcaa gaattccaaa
     2281 ctaaggccac agggaattct gaaatccgcg tagagatcgc accggctgat tttgtgctgg
     2341 ctgacagttc cgccaaaagc ctcaatatcc gacgtgacaa gctcgatcag ctctttttct
     2401 gacagttccc aatggagggc ttccgatgca aaggacacgt aaacatttgg actttgattt
     2461 gccgattcgt gtatggatat gaaaccatca tattcgggat atttaacatg ataacggtaa
     2521 ttgcggttcc cgcccgcata aaatatatgc ggccttgacc cttcttcttc gataatattt
     2581 tcaccctttc cataggcatt tgcttttcgt tcatcaaatc tctgtttcag ctcatcccaa
     2641 ttgtcgcccc aggtaacgta aaaaccgaca tccagggtat ctatgccgct agttagacga
     2701 tgtatgtttt caatgtctgt aattagcgca tggtgtgtta cagactccac catgctcttt
     2761 tgctcatcct tgcacgcgcc tgcttgcttg ctcgcgccct ctctaccggg cgcgccaagc
     2821 tgcgcgcttt cgattgtggt tttttgtgtt ttcataatta aaccaagtaa tcgtcgcaag
     2881 ccttataaaa acaaaaaggc tttgttcatg gatataaaat tgatcctgaa aaaagccttt
     2941 gattgttata tttattgatt gtctttattt gtgcgctttt tttatttttt taatttattc
     3001 tctccttatt attttcttat ttataattat tgcagtcagt atttaaattt tttatttgcg
     3061 ctattgtatt tttatttaat tgtctatgag ctctttccaa gctttatgcg acgataacct
     3121 ttcctccttt ctaatttgat cggcaaggtg taatttatga cgatgtcata gcttgccggt
     3181 tttttttgtt tcgattttca gtttcccgtc ttttcccacg taaatctttt cgctattgat
     3241 taaattttta atggttgtaa cgaccttatc agtgaaatgg gctgctttta gaattcctgc
     3301 cttgtgcagg atgttcatgt catccagtcc aatgacaatt agattccgca ttaatttgcc
     3361 tcgtgcaatg tcatatttct tcgcatagta atctattttt tttacgactt ccgggtccag
     3421 ctggacggaa tattgtttgt tttctcgttc tttcatgcgt cgcccatttt ttacttgagg
     3481 gctaaatact acattcatat ttcaatgtca agaagtaatt tatatatttt ccggcaccgg
     3541 ctccgacgag ccaagaaagg cccattctcg ccggtgccta cactgtgttt tgattatttg
     3601 attagtgatt ttttaggaga tggtttttcg ctgcctttta aaagagatga atgaggtttc
     3661 gaatcaattc taaaaaggtt catccgtctg atgtgtactt ttggcaataa atggtttttt
     3721 gaccaatcgt tttcacctac aaaggtgcag tcttttgcat ctgtcaaaaa taaggattac
     3781 tcatcgaatg caatgcctct atattttttt atcatttcca ctattcccaa tggataacca
     3841 ccagataaag ttgctaaatt gtagatatag ttttcagaat agttgatgtt gtgttttttt
     3901 gcatacatac taaataaatt tttaacatca tcttttttaa ggtggcttaa aattaatgtt
     3961 actgtcggca taagttgggc tttaatttca aataaatttt cttctggtag aaaatattct
     4021 acaatggcaa taaactgaaa atgctgctcc ataattaaat ggcgaagaaa aatctttttt
     4081 tgaacggtaa tttttgcaac gttgtcaaaa acaataacgg ggaggtgtct ttttgatgac
     4141 ttcatattag ctatacgata gcgcatggat ttatacccca tcttttcccc ttgcttgagc
     4201 ttcctggaga tagccacctc tttcataatt ttttcactca tttttccggg cgtttggctg
     4261 aaatcaacaa aaacaaattt tctttcatca gccataattc tagcgatttc tttaatcaag
     4321 cttgttcgcc caatcccgaa ctttccacca agaataatat ttttaccatc attcagtgag
     4381 tttataattt tctttttttc gcttttcctt cccacaaata acatcgttgc tctctaatat
     4441 aatttaatga acagacaaat ttagaatgat gctttttcta tctgaatgat cagtttttta
     4501 atttcgtctg tttgaccctt tgttccggaa attaagtttt ctattgttgc gagctcttca
     4561 tgaatataat tgattgcctg cagccgcatt tcttctatgg cgttattaac gctcttttcc
     4621 cactgatagg ccaagcggga aatattgata gttgcttctt tgggaacatt ggcaatgaaa
     4681 tgtcgttcga aaaccttccg aaatagaaac atgggaatga gaaaccatat caggtcaaga
     4741 tgaatatcaa aaggttttgt aaatgagatg tccggatggt tgggttccat gacatcaatt
     4801 ttccagtcca cctccgccat ctttactccg agcacagttt ctatattatc gttgagaaac
     4861 ttgcaaaaag cttccaacgc cctggaaaaa gatgcatgag ctttcttcat cgtgccaaga
     4921 aaatgactat gttcgttttt agaaattaaa cgcaactctt cggaaagcgt ttcagaaacc
     4981 caggtttcgt atttccggga tagcttccag aggttacctc tccaggaggt aatatccatc
     5041 tccaatttct ttatagcttt ctttgtcaaa ggcgagcgga attgctcaag gtatgtttca
     5101 atcattggac gagtttgtcg ctgatgttca cgggcaatga tgcccagttc ctcccgtaca
     5161 agatcttcat taaccttttc attaagaatt tgggcgcgaa gattctctct gttcatatct
     5221 gcctgcaggg atgactgcat agcaattttt aaatagccca ggcagctttc tgagagagaa
     5281 tggattttat aattaaggat tctaaaaaat tcagaatccc tatttttaca aattgttttt
     5341 aaaatatctt cttcaatgcg ttgtttatat tctttcgtgt tctctttaat tgaatagaga
     5401 taaacaggca agattctatg aagttcccgc tgaagcgtct gctgaaaaaa cgcaatgact
     5461 tcattttgct gttcaggtga aagaatgtca gccttggtca atagaagaat gatatttggc
     5521 gtatgactgg tcagttcacg aatgaggtct aaatcatgtt cagaaagtgg acggtcggaa
     5581 ctgacggcta aaatggctgt gccgacagag ggcagccatt cttcagacgt ggactggtga
     5641 tatttgtaaa cgctgcctaa tccaggagta tcaacgagac gtaagccctg gtaatcttgc
     5701 atggacggca attcaatatc cacaatagcg acgttttttg aattacccgg attttcagct
     5761 tccgatgtga attgcacaat ttcggccagg ggaatttcag taattgctcc gttgaaatgt
     5821 cttattaatg ctttttctct aatcccatat tgaacgcggg taatagccgt tgtaacggga
     5881 atggcgccta cgggtaaaac attttgacct ataaggttgt ttaaaaaaga acttttccct
     5941 gccttgaact gtcccagaac agctacgtct atgggtggat tttctgaaag cagtgtttgt
     6001 gcggcttcca gtttacgtgc aagagacacc atctggaaac gattgcatgc actggcaagt
     6061 atttctaaat gaccacggat gtcagaaata gatgtcgggg gcgaaatatt atccataata
     6121 aaaaactccc acagatgatt tttctgcagg agttatcagc aataattgcg gttacttcag
     6181 gtgaactcca tcaccgattc aaatatttga tcgtaaccat attttataat aattttttag
     6241 tcaatatgat tgttgctcag tgggcgagca ttataaagtg aaatagagca taagtattga
     6301 taataaattt tgcgactttc aggaagaaca tcgtgcgtgg atggagtacg caagattcgg
     6361 atgaaccaaa atattgaaag gtgtgtccct gagggtgtcc ttttttctga aattgagtga
     6421 taatgagtac aaaccattac aaataggggc actcagaccg tagtaatatc aaataattgc
     6481 aataaaacag gtgacttaga tatctttcag gtccccctct cacgccggaa accggggttc
     6541 aaacccccgt gggactacca aaataaaaac aataagttat attcagtaat gatgttatta
     6601 ttgccttgtt attattgcac tccccacagt attccctgga tgatatgact ccagcggaat
     6661 gtttgtcaaa gaacactaga aactctattt ttcaattaaa ggaatccgta tggtaaaagt
     6721 ggtccatggc attcatgttc atggatccgg tttaatcagc gggaagtcct tcgagccggg
     6781 ggaaatcgtt tatcggtttc caggcaaaat cagccaatcg gttccgacat atcaaacgat
     6841 ccagatcggc gcggaccgac atacccttga ttttggcatc ctggccaaca tcaatcattc
     6901 ctgtgatccc aatacggtca ttgatacgga aaacctgtct gtcttggctt tgcgcgccat
     6961 cgccaccgga gacgagctga ccttctttta tccttcaacg gagtgggata tggccctgcc
     7021 ttttgcttgt caatgccgct ctccacaatg tcttcagttt gtagccggcg caagatatct
     7081 cagccacggc gttctgaagg gctacttcat caacaggcat atcctcaaga tgcattccga
     7141 gctgctttcc ggggcatcag actcctgtgg gtttcagccg cggtaaggtg atgatcacgg
     7201 atgattccac tatagaacgg aggtgggaat gtgtcaccaa aatggccatt ggtagtgcat
     7261 ccaacattct gtggtcaagc ttttcttgac atacaagacc acgtttctta tacttcacac
     7321 cgcaaaaagg aagttcttat ccattcaatc acttcaatcc attgccgctt acggattgat
     7381 gaaagagggc cggctctccc tggaagaatt tctttccgcg ccggacgggt tcgatgatgt
     7441 ccgcgataaa gtccggggcg aaccggatca atatgaagcg atgggcagga cgcttgccga
     7501 cctgattctg gaagcaggag gcgggcgtct gccggatctg aagcgccgct gatttttatg
     7561 caagacaaaa gccgaccaaa tcgagattcc gatcggaacc gggaataccg ggacctgttt
     7621 aaattcatcg cctggatggt gggaacctcc gtgccggtga ccgccatgat cgttctgatc
     7681 ggcgtctatt cgggatcgct gacccttgtc gccatcacga tggactacgg cttgtcgcta
     7741 tgcctgaata tcatgtcgct ggccgcgctg ggcgtcgttt tgcgacagaa tgttttcaaa
     7801 tatccatacg gcgcgggcaa gctggaaaat tttacggcct ttgtttacgg cgtctgttta
     7861 atccccctgg ccatggccgt tttcaccttc gccgtgaaac ggtatttgaa tcccccgcaa
     7921 actctcaacc tgtggtttgc gctgctttac gtcgtcgccc tgatccgtct ggcggtgttt
     7981 gccgtctgga ttacacgtct ggggaaaagg catccgcggc attcacccct gatgcaggcc
     8041 tattatgcgg attaccgaat ttcgctggtc tatgaatccg ccattttcgc caccttgctg
     8101 atggggttat tgatatctga acacggcggc atgaagatcg cggtcatcat cgatgtgtcg
     8161 gtcgcgtcga tcatcgctct ttactatctg gttgttgcct gccggctgat catccgaaac
     8221 ttccgctcgc tgattgacct tccgctgggc gaaaaatacc agcgtcatat tctgcgctgt
     8281 ctcattgaag aattcgacgc gtatgacggt cttggcgcga tctatacgcg cagaagcggc
     8341 agcgaggtga tgatccagat tgagatgcgc tttgccgggg atacgccgct gaagcagatt
     8401 gacgacctgc gaaagcgcct ggaagaaaag ctgcgacaac tcgatgatca tgtcgttttc
     8461 caattgattc ccctgggccc ggaaacggcg ccgcgcgatc ccctggttga agaagttgaa
     8521 gaaaagaaat agcgggattc aaaacgcccg atggtaaggt tccatggaca atcagacaca
     8581 tacctgcttt ggaaaggtgc gaggatgatc gattttatgc cttttatcgg cgacggttgc
     8641 ggcaacacgt ttctggtttt atcgcagcgt tattgcgacc ggcacgccga tgaccacatt
     8701 cgccgtttgt ggaaggccac cggcgttgat tccgtcttga ttttgcaaaa atctcttcgc
     8761 ggcgacatcc ggatgctggt tcccggaaat gacggcattc ggggagacga aagcggggaa
     8821 ttctgtggaa acggggcgag agtggccgcc gcgttttacc tgcgtcattt ggggcaaagt
     8881 ccggtaatcg aaagccgcgg cggagtgctt ttccccacca tagttgagga atcggccgta
     8941 agcgttgaat ttccgttgcc ggaagagcga ggcggcattg tgcttgccgg gggagaaccg
     9001 catgtccgca cggcccggaa gatgacaccc cccgaactga tgcgattctg caatggaagg
     9061 tcccgtcggg tgagcgccaa ccgggtggcg atttgttcgt ctttgcatct gctcgtgcaa
     9121 acctttgagc attccattaa taaatttaca ggctgttgcg gaaccggatg cattgccatg
     9181 gcttatctgg cctatcggga aaggcgggtc gaacgccgtg ttaccgtcga agcgccgggc
     9241 ggacaattga ccgtggacat ccgaccatcc tgtatcatta tgtccggtcc ggtccgtctg
     9301 ataggatcga tggaacaatg gataaccgat cagacttagg aaccaagttt ttctactatc
     9361 gtaattcggc gtaattcaaa agatgccgat tgattttact ctgcgctttc ctgccttgtc
     9421 acccagtctt tcacttctgt aagcaggttt ttgcggaatg cttccggctc caaggtcgtg
     9481 atatgcggta tccatgacgg cttcctgtag tcaagcggtc gataattttt atggacgtcc
     9541 cacctttcaa tccg
//
LOCUS       DAOOLB010000076         9234 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_8732_length_9234_cov_42.253296, whole genome shotgun sequence.
ACCESSION   DAOOLB010000076 DAOOLB010000000
VERSION     DAOOLB010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9234
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8732_length_9234_cov_42.253296"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            84..872
                     /locus_tag="PLF77_08785"
     CDS             84..872
                     /locus_tag="PLF77_08785"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HPC08987.1"
                     /translation="MQKHISNVHVHMPYSLLPKYLKIILQNKLNLEIYFHHQILNRLD
                     KSRCMETAKILESEGLKITFHAPFVDLRPGALDETIRRASIDRIRQVFDLAPYFHPLK
                     IVCHASFDDRYYVYGDDLWLESSLKTWKEFIGLADDHRTIIALENVYEKNPVILGKLF
                     RSLASDRICFCFDTGHFNVFSCEPLNVWFDELGRYTGHLHLHDNFGWQDEHLSVGHGT
                     FPFAELMQYIRNMKKKPTVTLEAHNQKNLWASLDHVKRMDFPGV"
     gene            903..1877
                     /locus_tag="PLF77_08790"
     CDS             903..1877
                     /locus_tag="PLF77_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC08988.1"
                     /translation="MLRYVLKRTLFMIPLLIGITLICFFVMRLAPGSPTDLQTEMNPK
                     ASQESKERLNALYELDKPIYVQYGSWLKKISRGDLGVSFSSDHRPVADKIMERLPITI
                     LINFLSLVIILAVAVPIGVLSAVHQDSLFDKVTTVLVFIGFAVPTFWLALLLMIFFGI
                     HLGWLPISGLRSLNYEYLNVWNQLIDLAKHLVLPVFVSAFGGLAGLSRYMRANMLEVI
                     RQDYIMTARAKGLSERDVIYRHALHNALLPAITILGLSIPGLIGGSVIFETIFAIPGM
                     GQLFYMSVMARDYPTIMGILLIGAILTLVGNLIADVSYAVADPRIRIS"
     gene            1891..2718
                     /locus_tag="PLF77_08795"
     CDS             1891..2718
                     /locus_tag="PLF77_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPC08989.1"
                     /translation="MTAWTAIYKNKLMLTGGGLVLALLIVSILAPWLSPYDPGQIDLN
                     NVLAAPSMEHLFGTDQLGRDVLSRMIWGARISLKVGFVATGLAILVGTFLGALSGYYG
                     GWIDSVIMRFVDIMLCFPTFFLILAVIALLEPSIWNIMIIIGLTGWMGVTRLVRADFI
                     SLRERDFVQAARAIGAGDARIIFLHILPNAMASILVAATLGIAGAILTESALSFLGIG
                     VQPPTPSWGNILTAGKDNIDIAWWLSLYPGLAILTTVVGYNLLGEGIRDLLDPRLKK"
     gene            2812..2979
                     /locus_tag="PLF77_08800"
     CDS             2812..2979
                     /locus_tag="PLF77_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08990.1"
                     /translation="MPNRVNAESISDDKRILKRKLMQGEISEKDIQALIKKLPDVSEN
                     AEEVRLDDDKK"
     gene            2986..3765
                     /locus_tag="PLF77_08805"
     CDS             2986..3765
                     /locus_tag="PLF77_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPC08991.1"
                     /translation="MLIDSHAHLEMKEFDADRDDVISRARSEGVDFIVTVGTNLRLSS
                     RAVALAEQYENIFATVGIHPHDVAKAGEDTLDALKQLVLTHKKIVAYGEIGLDYFRNI
                     SRQEIQVEMFGQQLDLVKELNLPVVIHDREAHRQSLDMIQASGVRRGVFHCFSGDYDM
                     ARKCIDLGFYISIPGVVTFDKSGKIRDVVERVPLSSLLLETDAPYLTPVPNRGKRNEP
                     AFIVHTAKAVAAIKKISWEDVAAQTSVNTINLFGLKMIRNV"
     gene            3786..4481
                     /gene="queC"
                     /locus_tag="PLF77_08810"
     CDS             3786..4481
                     /gene="queC"
                     /locus_tag="PLF77_08810"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HPC08992.1"
                     /translation="MKKQFKAVVLLSGGLDSATTLAIARDRGFEIYALSFRYGQRHAV
                     EMKAARRIARKNKVAGHLILNMDLRKIGGSALTADIHVPKNQTGSVLKRKIPVTYVPA
                     RNTIFLAYALAWAEVIGSHDIFIGVNALDYSGYPDCRPRYIAAFRKLANLATKAGVEG
                     KQKLAIHAPLIKMSKAEIIKKGMDLGVDYSLTHSCYDPSPSGEPCGECDSCLLRLKGF
                     REAGLKDPLKYCR"
     gene            4492..5133
                     /gene="queE"
                     /locus_tag="PLF77_08815"
     CDS             4492..5133
                     /gene="queE"
                     /locus_tag="PLF77_08815"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR04508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HPC08993.1"
                     /translation="MGYRIKEIFYSIQGEGFYTGRPAVLCRFSGCNLWSGKASDREKA
                     VCRFCDTDFVGCDGPEGGIFQTPEALTEKIITLWPEKPSDTLRPFVVLTGGEPLLQTD
                     RALLDALHHQHVEVAVETNGTIMPPEGIDWICVSPKAGTTMVLRSGQELKLVYPQQGA
                     EPEKYALLDFQHYFLQPLDSPNRQTNTLRALEYVLAHPQWRLSLQIQKILNVK"
     gene            5145..5504
                     /gene="queD"
                     /locus_tag="PLF77_08820"
     CDS             5145..5504
                     /gene="queD"
                     /locus_tag="PLF77_08820"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HPC08994.1"
                     /translation="MEIFKVYKFDAAHRLPKVGAEHKCFELHGHSFRVEIHVQGPVDP
                     QLDWVMDFADIDRAFRPLLDQLDHQNLNDIPGLNNPISENIARWIWENLQDALPQLNK
                     IVVQESPESGCIYCGEK"
     gene            5663..6064
                     /locus_tag="PLF77_08825"
     CDS             5663..6064
                     /locus_tag="PLF77_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08995.1"
                     /translation="MDQKAIQKIKGTQQDGLLEELLREKAAVLTRASWAVEDVLEKMK
                     RIEGEIEIKTSLIQSMTHEADRAVLSRQKKLFCEEINCKIDEFNAIRRKAQLQYYYLI
                     VTREALGLRRHEMVQKIYRIPDKKKKIQVFF"
     gene            6064..6717
                     /locus_tag="PLF77_08830"
     CDS             6064..6717
                     /locus_tag="PLF77_08830"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPC08996.1"
                     /translation="MDRFRKPRMRMVETQIKARGISDVRVLNAMQTIPRHLFVDEGLI
                     EQAYTDSPLPIGEHQTISQPYIVALMTQALDLKGRERVLEIGTGSGYQTAVLACLADR
                     VFSVERIALLATQARRTLDALNIYNVAIRVGDGSYGWKEEAPFDAIITTAAVPAIPRY
                     LLDQLVPGGRMIVPSGGRDVQTLYKLTRSKENPEEIIQEDLGGCRFVSLIGESGWRK"
     assembly_gap    6833..6932
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6933..7418
                     /locus_tag="PLF77_08835"
     CDS             <6933..7418
                     /locus_tag="PLF77_08835"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPC08997.1"
                     /translation="TGDLQDSATAKIRDDKPTDPKNQTVEEMVVPDRKIKATRNKFIW
                     PVQGEIKAHFGIQPNKTLHNWVKIVSASGKKIKAAESGTVIFSSHLKNYGETIIIRHK
                     DHFATVYTHLKKRLVKMDTPVKKGEAIAVLGEKDDDGAVYMNFEVRLKGKAVNPLSYL
                     P"
     gene            7483..8628
                     /locus_tag="PLF77_08840"
     CDS             7483..8628
                     /locus_tag="PLF77_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC08998.1"
                     /translation="MKCYFEQGPIRPPSEAASLLIRVSRNCPWNKCAFCHTYRGTKFE
                     LRSVEDVKKDIQNMKEIADEIVRFSRKLEEGGRVNRNVLRAIYNSEYFYHENIYSVAA
                     WLYFGGENVFLQDANSLILKTPDLVSILDFLKEKFPDVQRITSYCRSHTAARKSIAEF
                     EQLKKAGLSRIHIGMESGSDEVLSFIRKGVTSADHIRAGLNIRGAGIELSEYVMPGLG
                     GEQFTRQHALETARVINAINPDFIRLRTLHVVPGTDLDELMNKGEFKPLADDDILREI
                     KLFIENLDVRGTYLASDHILNLLEELEGSLPDDRQNMLAAIEKYFKLSAEDRLVFRLG
                     RRSGSLRKLDDLADQATYGRLRSVVDHYAARGENLDEDIIKMMNNFI"
     gene            9005..>9234
                     /locus_tag="PLF77_08845"
     CDS             9005..>9234
                     /locus_tag="PLF77_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC08999.1"
                     /translation="MRECVIIDGVRSPNSRAHNEKGWFRALRPDEVLTKVYDGLFARN
                     PKVKPEDIESVFVGCANITGMQNDIGRLAWLAG"
BASE COUNT         2293 a         2088 c         2452 g         2301 t
ORIGIN      
        1 acatttgatt gttgtgaaaa aaaaacggct gtggtaactt cttgcgcaat tcgatttttc
       61 aaagcgcaca agggtgtttt ttcatgcaga agcatatcag caacgttcac gtgcacatgc
      121 cttattctct tttaccgaag tatttgaaga tcattcttca gaacaaactg aatctggaaa
      181 tatactttca tcaccagatt ctgaatcgtc tggataaatc tcgatgcatg gaaacggcaa
      241 agattcttga aagcgaggga ttgaaaataa cgtttcatgc gccttttgtg gacttacggc
      301 cgggggccct ggatgagacg atccgccggg ccagcattga ccgcatcagg caggtttttg
      361 atctggcacc ctattttcat cccctgaaaa ttgtctgtca tgcgtctttt gacgaccgtt
      421 actatgttta tggcgacgat ttatggctag agagcagcct caaaacctgg aaggagttca
      481 tcgggctggc ggacgatcac cggaccatca ttgcgctgga aaatgtttac gaaaaaaatc
      541 ccgtgatatt gggcaagctt ttccgatctc tggcgtccga cagaatctgt ttttgtttcg
      601 ataccggcca ttttaatgtg ttttcctgcg aaccgttgaa tgtctggttc gatgaactgg
      661 gaaggtatac ggggcatttg catctgcatg acaatttcgg atggcaggac gagcatcttt
      721 ctgtcgggca cggcacgttt ccctttgcgg agttgatgca gtacatcaga aatatgaaga
      781 aaaaaccgac ggtcacgctg gaggcgcata atcagaaaaa tctttgggca tcgctcgatc
      841 atgtcaaacg aatggatttc cccggtgttt gaagataaga cggactgtgt tgggggagta
      901 ttgtgctgag gtatgtctta aaaagaacat tgttcatgat tccgctgttg atcgggatta
      961 ccctgatctg ttttttcgtc atgcgtctgg cgccgggttc tcccacggat ttgcagacgg
     1021 agatgaatcc gaaggcatcc caggaaagca aagagcggtt gaatgcgctg tatgaactgg
     1081 ataagccgat ttatgtgcaa tacggatcat ggctgaagaa gatcagccgc ggcgacctgg
     1141 gcgtatcctt ctcgtccgat caccgtccgg tggccgacaa gatcatggag cggctgccca
     1201 ttaccatcct gatcaatttt ttatcgctgg tgatcatcct tgctgtcgcg gtgccgatcg
     1261 gcgtgttgtc cgccgtccac caggactcgc ttttcgataa ggtgaccacc gtgctggtgt
     1321 ttatcggttt tgcggtgccc acgttctggc tggccctgct gctgatgatt tttttcggaa
     1381 tccatctggg ctggctgccg atttccggtc tgcgctcgct caattacgaa tatctgaatg
     1441 tctggaatca gttgatcgat ctggccaaac atctggtttt gccggttttc gtgtcagcgt
     1501 tcggcggatt ggccggtctg tcgcgttata tgcgggcgaa tatgctggag gtgattcggc
     1561 aggattatat tatgaccgcg cgcgccaagg gattgagtga aagggatgtc atttaccggc
     1621 acgccctgca caacgcgcta ctgccggcca tcactatttt ggggctttcc attcccggtc
     1681 tcattggcgg cagcgttatc tttgaaacaa tttttgccat tccgggcatg ggacagcttt
     1741 tttacatgtc ggtcatggca agagattatc cgaccattat gggaattttg ctgatcggcg
     1801 ctattctgac gctggtgggt aatctgattg ccgatgtgtc gtacgcggtg gcggacccgc
     1861 gtatcagaat atcctaaaag ggctgaaaag atgacggcat ggacagccat ctataaaaac
     1921 aaactgatgt tgaccggcgg cggactggtt ctggcgcttt tgatcgtttc cattctggcg
     1981 ccgtggctgt ctccctatga tccgggacaa atcgatttga acaatgtgct ggcagctcct
     2041 tctatggagc atcttttcgg aacggatcaa ctggggcggg atgttctttc gcggatgatc
     2101 tggggtgcca gaatttcttt aaaagtcggt tttgtggcga cggggttggc cattctcgtc
     2161 ggcacgtttc tgggagcatt gtcgggatat tacggcggct ggatcgattc ggtcatcatg
     2221 cggtttgtag atatcatgct ctgtttcccc acgttctttc tgattctggc ggtcattgct
     2281 ttgctggagc cgtccatctg gaatattatg atcattatcg gactgaccgg ctggatgggc
     2341 gtaacaaggc tggtccgggc ggattttatt tcgttgcggg aaagggattt tgtccaggcc
     2401 gccagagcga taggcgccgg ggatgcgcgg attatttttc ttcatatcct tcccaacgcg
     2461 atggcgtcga ttctggttgc ggcgacgttg gggattgcgg gggcgatcct caccgaatcg
     2521 gccctgagtt ttctgggtat cggtgtgcag ccgcccaccc ccagctgggg caatattctg
     2581 acggccggca aggataatat cgacatcgcc tggtggctgt cgctttatcc gggcctggcc
     2641 attctgacga ccgttgtcgg ttacaatctt ctgggggaag gcatcaggga tctgcttgat
     2701 ccccgtttaa aaaaataaaa ccggggcctt gtcaaccggt tcattcttga taaaaggacg
     2761 aaaatataat aataacctca caacgaccta tacgtaagga gaagaaacca tatgcccaac
     2821 agagtcaatg cagaatccat cagtgacgat aaacgcatcc tgaagcgcaa gctgatgcag
     2881 ggcgaaatat ccgaaaagga tatacaggct ctaataaaga agcttcccga tgtttccgaa
     2941 aacgccgagg aagttcgcct ggatgatgac aaaaaatagt attttatgct gattgattcc
     3001 catgctcatc tggaaatgaa ggagtttgat gcggaccgtg atgacgtcat tagccgcgcc
     3061 cgaagcgagg gcgtagattt tattgtcacg gtcggaacca atctccggtt gagcagcaga
     3121 gccgtcgcgc ttgccgaaca atacgaaaac atctttgcca ccgtcggcat tcatccccac
     3181 gatgtcgcca aagccggtga ggacaccctg gatgctttaa agcaacttgt tctgactcat
     3241 aaaaaaatcg tcgcctatgg tgaaatcgga cttgattatt tccggaatat ttcacgtcag
     3301 gaaatacaag tcgagatgtt cgggcagcag ctggacttgg tgaaggaact gaatctgccg
     3361 gttgtgattc atgatcgtga agcgcatcgt caatcgctgg atatgattca ggcctccggt
     3421 gtgcgtcgcg gcgtctttca ctgtttttcc ggggattatg acatggccag aaaatgcatc
     3481 gacctcggat tctacatctc catccccggt gtggtcacct ttgacaaatc cgggaaaatt
     3541 cgcgatgtcg tcgaacgggt tccgctttcc tccctgttac tggaaacgga cgcgccgtat
     3601 ctgacgccgg ttccgaaccg cggcaaaaga aacgaacccg cctttatcgt ccatacggcg
     3661 aaagcggtgg ccgccataaa aaaaatatca tgggaagacg tggcggcgca aacgtctgtt
     3721 aacacgatca atctcttcgg tctgaagatg atccgtaacg tttagttttt tacggagccc
     3781 cgttcatgaa gaaacaattt aaagccgtgg ttttgttaag cggcggtctt gattccgcca
     3841 caaccctggc gatcgcccgc gacaggggat ttgaaattta cgcgttgagt ttccgctacg
     3901 gccagcggca cgcggtggaa atgaaagctg cccggcgtat tgcacgaaaa aacaaggtcg
     3961 ccggacacct gatcctgaat atggatttac gcaaaatcgg aggatcggcc ctgacggcgg
     4021 atattcatgt gcccaagaat caaacaggct cggtgctgaa aaggaaaatt ccggtgacct
     4081 atgttccggc gcgcaacacg atatttcttg cctacgcgct ggcctgggcg gaagtgatcg
     4141 gcagtcatga tatctttatc ggggtcaatg ctctggatta cagcggttat ccggattgtc
     4201 gtccccggta cattgccgca tttcgaaagc tggccaatct ggcaacgaag gcgggggttg
     4261 aaggaaaaca aaaactggcc attcacgctc cgctcattaa aatgagcaag gcggagatta
     4321 ttaaaaaggg catggacctt ggcgtggatt acagtctgac gcacagttgt tatgatccgt
     4381 cgccatcggg agagccgtgc ggtgaatgcg attcctgtct gctgcgattg aaagggtttc
     4441 gtgaagccgg attgaaagat ccgctgaaat attgccggta aggggctggt cttgggttat
     4501 cgaatcaaag agatatttta ttccattcag ggcgagggat tttatacggg acggcctgcg
     4561 gttctctgcc gtttttcggg gtgtaatcta tggtcgggga aagcatccga ccgcgaaaaa
     4621 gcggtttgcc ggttctgcga tacggacttt gtcggttgcg acggcccgga gggcgggatt
     4681 tttcaaaccc ctgaagcatt aaccgagaaa attattacat tgtggcctga aaaaccgtca
     4741 gacacgctta ggccgtttgt cgtgctcacg ggaggagaac cgctgctgca aacggataga
     4801 gccttactgg acgctctgca tcatcagcat gttgaagtcg ccgttgaaac caacggcacc
     4861 attatgccgc cggaaggaat tgactggatt tgtgtgagcc ccaaagcagg cacaacgatg
     4921 gtgttacgtt cgggacagga attaaagctc gtttatcctc agcagggagc cgagccggaa
     4981 aagtatgcgc tgctcgattt tcagcattat tttctgcagc cgcttgattc accaaaccgg
     5041 caaaccaaca ccctcagggc cctggaatat gttttggcgc atccgcaatg gcggttgagt
     5101 ttgcagatac aaaaaatttt aaatgtaaaa taaaggaacg caaagtggaa atcttcaagg
     5161 tgtataaatt tgatgcggcg caccgcctgc cgaaggttgg tgcagaacac aaatgttttg
     5221 aactgcacgg acattccttt cgcgtggaga ttcatgtgca aggtccggtc gatccgcagc
     5281 tggattgggt gatggatttt gccgatatcg accgtgcttt ccggcctctg ctggatcagc
     5341 tggatcatca gaatttgaat gacatccccg gactgaataa tcccatctcg gaaaacattg
     5401 cgcggtggat ctgggaaaat cttcaggacg cattgccgca attaaacaaa atcgttgtgc
     5461 aggagagccc ggaatccggc tgcatttatt gcggcgaaaa ataacggttt ggaggcgggg
     5521 atgctccgca gaaaaaattt ccgtttagcc gccgaaacgt cacggatgtc ttccgtttca
     5581 gagcggttta acagtccctt ttttgttgaa aaataatgcg gtcggtggta ggtgctgaaa
     5641 caaacaaaaa gcagtgacca tcatggatca gaaggccata cagaagatca aaggcaccca
     5701 gcaggacggt ttgctggaag aattattgcg ggaaaaggcc gcggtgctga cccgggcgag
     5761 ctgggcggtt gaggatgtgc tggagaagat gaagcgaatc gaaggagaaa tcgaaattaa
     5821 aacatcgttg atccagtcga tgacgcacga ggcggatcgc gcggttctgt cccgtcagaa
     5881 aaaactgttc tgcgaagaaa taaactgtaa gatagacgaa ttcaatgcca ttcgccggaa
     5941 agcccagctt caatattatt atttgattgt cacaagagaa gccctgggct tgaggcgtca
     6001 tgagatggtt cagaaaattt accgtattcc cgacaagaaa aaaaagatac aggtgttttt
     6061 ttaatggaca ggttccgtaa accacgcatg cggatggtag aaacgcagat caaggcacgt
     6121 ggcatcagcg acgtgcgcgt gttgaatgcc atgcaaacga ttccgcgcca tctttttgtc
     6181 gatgaaggcc tgatcgaaca ggcgtacacc gacagccctc tgcccatcgg cgaacatcag
     6241 accatctccc agccgtatat cgtcgcgctc atgacgcagg cgctggatct gaagggaagg
     6301 gaacgggtgc tggaaatagg aacgggatcc gggtatcaga ccgcggtgct ggcctgtctg
     6361 gcggaccgcg ttttttccgt agaacgcatt gccttgctgg ccactcaggc gcgcagaaca
     6421 ctcgacgcgc tgaatatcta taatgtggcc atcagggtgg gtgacggttc ctacggctgg
     6481 aaggaagaag cgccgtttga cgccattatt acaaccgccg cggtgccggc gattccccgg
     6541 tatctgctgg atcagttggt tccgggggga agaatgattg tcccgtcggg tggccgcgat
     6601 gtgcaaaccc tctataaact aacccgatcc aaggaaaatc ctgaagagat tatacaagaa
     6661 gaccttggag gctgccgctt cgtcagtttg ataggggaga gcggatggag aaaatagcaa
     6721 cgcgggcaaa accggttttt tttcttctgc tggtctgtct gattttttcc tgctggcatc
     6781 cccggctgca aaaggcccag gcggcgggcg tttatcattt ggtgaaaaag cannnnnnnn
     6841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaccggcga tcttcaagat tccgcaacgg
     6961 caaaaatccg ggacgacaaa ccaacggatc caaaaaatca aacggttgaa gaaatggttg
     7021 tcccggaccg gaaaataaaa gcgacacgca acaaatttat ctggccggtg cagggcgaaa
     7081 tcaaggcgca tttcggcatt caacccaata aaacgctcca taactgggtt aaaatcgttt
     7141 ctgcgtcggg aaagaaaatc aaagcggcgg aatccggcac ggtcattttt tcatcccatc
     7201 tgaagaatta cggtgaaacc attattatcc gtcacaaaga tcatttcgcc acggtctaca
     7261 cccatctgaa aaaaaggctg gtcaaaatgg atacgcctgt caaaaaaggc gaggcgatag
     7321 ccgtcctggg ggaaaaagac gatgacgggg cggtttatat gaattttgaa gttcgtctga
     7381 aagggaaagc ggtcaatccg ctttcatatc tgccgtaatt ttttgctgtt gccatgacgc
     7441 aaatcatcct tatattaaat aaaaaaatta ttcaggttaa cgatgaaatg ctattttgaa
     7501 cagggcccca ttcgtcctcc cagtgaagcg gcaagccttc tgatccgcgt cagcagaaat
     7561 tgtccgtgga acaaatgcgc tttctgtcat acctatcggg ggacgaaatt cgagctgcgc
     7621 agcgttgaag acgtcaaaaa agacattcag aacatgaaag agatagccga tgaaattgtc
     7681 cgtttttccc ggaaactgga ggagggcggc cgggtcaacc gcaatgtttt gcgcgccatc
     7741 tacaatagcg agtattttta tcatgaaaat atttattctg tggcggcctg gctttatttc
     7801 ggcggtgaaa acgtgtttct gcaggatgcc aattcgctga ttttaaaaac cccggatctt
     7861 gtttccattc ttgatttttt aaaggaaaaa tttcccgatg tgcaacggat aacatcgtac
     7921 tgccgttccc acacggcggc aaggaagagc attgcggaat tcgaacaatt gaaaaaagcc
     7981 ggactgtccc gcattcatat cggcatggaa agcggctccg acgaggtatt gtccttcatt
     8041 cgcaagggag tgacgtctgc cgatcatatc agggcgggac tgaacatcag aggcgccggc
     8101 atagaattaa gcgaatatgt catgcccgga ctgggcggag aacagtttac ccgtcagcat
     8161 gctttggaaa ccgcccgggt catcaatgcc atcaatcctg attttatccg tctgcgcacc
     8221 ctgcatgtgg tgccgggcac cgatctggat gagttgatga acaaaggtga attcaaaccg
     8281 ctggcggatg acgatattct ccgggaaata aaattgttta ttgagaatct tgacgtgcgc
     8341 gggacgtatc tggccagcga tcatattctg aatctgctgg aggaactgga ggggagtttg
     8401 ccggatgacc ggcaaaacat gctggctgcg attgaaaaat attttaaact ttccgccgaa
     8461 gacagacttg ttttccgact gggacggcgc agcggatcat tgaggaaact cgatgatctg
     8521 gccgatcagg caacatacgg gagactgagg tcggtcgtcg atcactatgc tgctcgaggc
     8581 gaaaatcttg atgaggatat catcaaaatg atgaataatt tcatataaat atcggggaaa
     8641 tatcttgtcg tccgtacagc cttttccttt atgtcatcct gattcgtttt attttgccat
     8701 gtggtaaata ttttattgac acgaaggttc tttctgctta tacttccgcc gtcaaaaggc
     8761 aatattttat cgcgtatcag gcaatcggcg tctcgcaccg cgtctgctga tgatctgaga
     8821 ccattttatt cctggcttcg aaagagcttg actatttaca gctccataat ggtcggcaga
     8881 aaagttattc tgcagaacat tattcaatgc tacaggaatg tgatccgcag gattgcccgg
     8941 taggttgata aaaggatgcg acgttttgat aaaatatcaa acaataattt aaggaggcta
     9001 tacaatgcgt gaatgtgtaa ttattgatgg cgtgagatcg ccaaattccc gggcacacaa
     9061 tgaaaaaggt tggttcagag ccctcagacc cgatgaagtt ctgaccaaag tttatgatgg
     9121 tctgtttgcc cgtaacccca aagtgaaacc cgaagatatc gaatccgtat ttgtcggttg
     9181 cgccaacatt accggcatgc agaatgatat cggccgcctg gcctggctgg ccgg
//
LOCUS       DAOOLB010000077         9166 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_8826_length_9166_cov_33.483701, whole genome shotgun sequence.
ACCESSION   DAOOLB010000077 DAOOLB010000000
VERSION     DAOOLB010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 9166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9166
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8826_length_9166_cov_33.483701"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="PLF77_08850"
     CDS             complement(<1..575)
                     /locus_tag="PLF77_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPC09000.1"
                     /translation="MEYKNVDDVQSSLTDAGYIPSREIATVVFLAAATQKPVLVEGPA
                     GVGKTELAKAIARSLERDLIRLQCYEGLDEAKALYEWEYAKQLLYTQMVKDRLNDIIS
                     SAATLTEAVDQIAAQEDAFFSDRFIQHRPLLQAISSEKPVVLLVDEVDKADPEFEAFL
                     LEILSDFQVSIPELGTRKAIQIPFVLLTSNN"
     gene            858..2240
                     /locus_tag="PLF77_08855"
     CDS             858..2240
                     /locus_tag="PLF77_08855"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPC09001.1"
                     /translation="MTDSPIKSALKDFFFFWKPSLARRLTLSFTIFGLVIGYAVLIYL
                     AVSSTNTLIRQASVSVEEYLTYMSREDLRENQGELLKFIDQKMTNIVHMAKAMQVVLL
                     PVQFELYFKKDGQWQHTFTDEQGIVTSEIISNPGLAGLLEVAKSRKMTTSSKFFYGRH
                     DKVNVKINISPDKNNYTQLLAFDIYRVGILKILRENIYKAFFFFVLLLVICHMMGHIF
                     SYLLARPIEQLSREAEAIASGQYERRFTSNRQDEIGKLADALNIMASRILDSTKEREN
                     IFIGILIALTRAIDAKSPWTAGHSERVTKFAEAIGHNLHFNEDEMRALTISAILHDIG
                     KIAVPEQILDKPGKLTDEEFAVVKKHPQAGADIISSIPSYESILEGIVHHHERWDGKG
                     YPKGLHEKDIPLIARIICIADVYDALTEDRPYRRAWSRTEVRQFFEEQKGKMFDAELV
                     DVFLTLPDVQ"
     gene            complement(2373..2522)
                     /locus_tag="PLF77_08860"
     CDS             complement(2373..2522)
                     /locus_tag="PLF77_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HPC09002.1"
                     /translation="MCSVCGYVYDPVKGDPDNGIAAGTKFEDLPDSWTCPICGADKSK
                     FEKEK"
     gene            complement(2625..3065)
                     /locus_tag="PLF77_08865"
                     /pseudo
     CDS             complement(2625..3065)
                     /locus_tag="PLF77_08865"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            3263..3853
                     /gene="fabA"
                     /locus_tag="PLF77_08870"
     CDS             3263..3853
                     /gene="fabA"
                     /locus_tag="PLF77_08870"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HPC09003.1"
                     /translation="MKYQEFLKRARFDFEEILAFAYGKLVDDPPEHFDAKFPAPPFLM
                     VDRILSIESDGKKGKIIAEQDIRPDAWYFQCHFQGDPVQPGCLGVDGLWQLLGFFCVW
                     RGALGTGRALGCGDVAFNGQIRPFNKCVRYEVDVRRYSMLKESGASIVIGDGRVYVDH
                     ELIYTVGQARVGVFKDIAYKDYPRRSKYSIGGIMER"
     gene            3938..4354
                     /locus_tag="PLF77_08875"
     CDS             3938..4354
                     /locus_tag="PLF77_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09004.1"
                     /translation="MNIVKKTAAYFARKEFCRNAINECADLSLLKEKHTLRVTIGIVL
                     IVLSYIVVLPSFFMVGVMAARLRKPMVGVIGIPLAYGFSWLIMMMGMYLTGPEYAKTL
                     GKYVVRVVLEKILGDEAKKIASKPNKNIDKTGEPNE"
     gene            4347..4619
                     /locus_tag="PLF77_08880"
     CDS             4347..4619
                     /locus_tag="PLF77_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPC09005.1"
                     /translation="MSNAAEMIRELKIKIIETLGLIDVGPDDIQDDERLVGGDLGIDS
                     IDVLELVMMIEKDYGVKIDSKKLGVKVFASVSALADHILNHQKKTV"
     gene            4651..6003
                     /locus_tag="PLF77_08885"
     CDS             4651..6003
                     /locus_tag="PLF77_08885"
                     /inference="COORDINATES: protein motif:HMM:TIGR04072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid biosynthesis B12-binding/radical SAM
                     protein"
                     /protein_id="HPC09006.1"
                     /translation="MMNVLLISANTLKNPYPVYPLGLDYVAGCLDYRYRTKIVDLNVF
                     PHPETLEAMIREFSPDVVGISIRNIDNTDAINCRSFLTEYETLVGQIRKNSRAKIILG
                     GSGFTIFPREFMQALDADYGIVGEGERLALLLDAWEKNEDVNKIPGVLTSVKTDIVYE
                     PFGGRLNRHFDPEETHTRFYLAYGGMLNLQTKRGCPFRCGYCTYPHIEGGKMRCYDPE
                     EIARSARALQDAGAKYIFITDSAFNASYEHSAQVAKAFTKAGVSIPWGGFFASTVPPA
                     DYFKRLADAGLTHVEFGTESLSDQVLENLRKPFTSENVFTAHREALDAGLYVAHYFLL
                     GGPGENKETLQATLMGIDKLERAVFFFFCGIRIYPHTALYDVAVREGQIKAGENILPP
                     VFYRSQGIRVEEIMKTVENHANGRLNWLIGAGEAKATRILPKLYRRGFTGPLWEYLIQ
                     "
     gene            6209..7009
                     /locus_tag="PLF77_08890"
     CDS             6209..7009
                     /locus_tag="PLF77_08890"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPC09007.1"
                     /translation="MKKKLSSANPAEMTGVILLLAAAVIFFFHPLAAAAVALFYIVLC
                     VAASFFPQSSFYLPVISRGRTGRNVVALTFDDGPAEPTTRRILDLLDRYSVKATFFVS
                     GVNALKHPDIVREIIRRGHTVGNHSFHHNPFLMLGSYRYLYNEVSKAQDILKDRGVNT
                     LIFRPPVGIISPKLPSVLDKLGMFCVTFSCRGGDFGNRRIRNLSSRILHSVKADDILL
                     LHDALPRGGGNQAVLLVEIEKILRGLKKQGLNVVPLADLIGRHVSIMM"
     gene            7137..7508
                     /locus_tag="PLF77_08895"
     CDS             7137..7508
                     /locus_tag="PLF77_08895"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09008.1"
                     /translation="MKNQWNLLKETREADSNDIEALADVPADSPWFSGHFPGEPILPG
                     IALVYAVKQAIHREAQKKGERLELHTLKRIRFMQPVRPGDQLSLNIRAAEADGERLFH
                     FKIFCRDTMVSSGMIAAKKMK"
     gene            7548..7820
                     /locus_tag="PLF77_08900"
     CDS             7548..7820
                     /locus_tag="PLF77_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPC09009.1"
                     /translation="MPASQDVKGIIRRVAEIIIDELKLEEATADTFDPDMDLVDELGI
                     DSMDLATIALVLQDEYRITIDEDDYPQLKTIRLISEYIKERLPVRH"
     gene            7836..8951
                     /locus_tag="PLF77_08905"
     CDS             7836..8951
                     /locus_tag="PLF77_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09010.1"
                     /translation="MANQVNNKVNTESVKPKFSELLARPEVKARWEKVRKYFFLREST
                     YDMTNRCNIRCDGCYYYEGEKQFAKEVGDVEAWRALMRAEKARGITYVVLAGAEPSLV
                     PELLEVCYQEMPLGSIATNGFRKIPPSVGYKLHISVWGSDETSLKIRKAKNLLKKQIE
                     NYENDPRAVFVYTFTRNNIDEVNEVTEELVAHNCKLTFNVFSAPVGYAGNLRHDDASL
                     EQTRQTMIELLNQYPRNVLFSVYNAIAHTHRKGLHDLYSCSYPRQNPSQDIGLGRSFR
                     QYRTDLNWDRSVACCVPDTDCADCRHYAAGSAVVTARLYRHVTNLATFKSWLDYVDTY
                     LAVWVMNYEKGENLCNEIIEPPRAAVEKKQNVPLHPK"
BASE COUNT         2398 a         2049 c         2335 g         2384 t
ORIGIN      
        1 tagttattgg atgtcagcag cacaaaggga atctgaatgg ccttgcgggt gcccagttcg
       61 ggaatggaca cctgaaaatc acttaatatt tccagaagaa atgcctcaaa ttccggatcg
      121 gccttgtcca cttcatccac gagaagcacg acaggttttt ccgaactgat cgcctgcagc
      181 agcggccgat gctgaataaa acggtcggag aagaaagcgt cttcctgcgc ggcaatttga
      241 tccaccgcct ctgtaagagt ggcggcggaa gagatgatgt cgttgagtct gtccttgacc
      301 atctgggtgt aaaggagctg tttggcgtat tcccattcgt aaagcgcctt ggcttcgtcc
      361 agaccttcat aacattgaag ccggatcaga tcgcgctcca gggagcgggc gatggctttg
      421 gccagttccg ttttgccgac gccggccggt ccctcgacca gcaccggttt ctgggtcgct
      481 gccgccagaa aaacgactgt cgcaatttcc cgcgacggaa tatatccggc atctgtcaaa
      541 ctggattgaa catcgtctac gtttttgtat tccataagag aatgaattcc ttttcgaatt
      601 gcgcaaagca taggtgattt atcagggtat gtcaatcagg aatcgagatc caacaccgac
      661 tcaaggagta tatattctca gcatcagaga gttcctgaat ccgctttgtt tcgcgtcggg
      721 gataatcaaa tctactggat cattcgacca gcgcttccta ccgtcaacaa cgatcatgaa
      781 aaagaatttt tctcagccat cctttcatgc tatgataaac caaaattgat ttgaaatagg
      841 aaaataacgt ctctgaaatg acagacagcc ccataaaatc agccctgaaa gattttttct
      901 ttttctggaa accttccctg gcccgaaggc tgacactttc ttttacaatt ttcggcctgg
      961 tgatcggtta cgccgttctc atttatctcg cggtttcttc aaccaacacg ttaatcagac
     1021 aggcatcggt ttcggtagaa gaatacctga cgtacatgtc cagggaagat ctgcgtgaaa
     1081 atcagggcga acttcttaaa ttcattgatc agaagatgac caatattgtt catatggcaa
     1141 aagccatgca ggtggtgctg ctgcccgttc aattcgaact ttattttaag aaagacgggc
     1201 agtggcagca tacgtttacc gatgaacagg ggatcgtcac atccgaaata atcagcaatc
     1261 ccggacttgc cggattgctg gaagtggcaa aaagtcggaa gatgacgaca tcgtcaaaat
     1321 ttttttacgg acgacatgat aaagtcaatg tgaagattaa tatcagcccg gacaaaaata
     1381 attatacgca acttctcgcc tttgacattt accgtgtcgg catcctcaag attcttcggg
     1441 aaaatatcta caaggcgttt ttctttttcg ttttgctgct cgtgatttgt catatgatgg
     1501 ggcatatctt cagttacctc ctggcgcggc ccatagaaca actatcccgg gaggcggagg
     1561 ccatcgcatc gggacaatat gaacgccgtt ttacgagcaa tcgtcaggat gaaatcggca
     1621 aactggcgga tgcgctcaat atcatggctt cccggattct ggattcgacc aaggaaagag
     1681 aaaatatttt tatcggcatc ctgattgctt tgacgcgggc cattgatgcc aaatcgccgt
     1741 ggacagccgg tcacagtgag cgggtgacca aatttgcgga agccatcgga cataatcttc
     1801 atttcaatga ggatgaaatg cgcgccctga cgatttccgc tattctccac gatatcggga
     1861 agattgccgt acccgaacag attttggata aaccgggaaa attgaccgac gaggagtttg
     1921 ctgtcgtcaa gaagcatccg caggccggcg ccgatatcat ttcttccatt ccctcctatg
     1981 aatcgattct tgaaggcatt gtgcatcatc acgagcgttg ggacggaaaa ggatatccca
     2041 aagggcttca cgaaaaagac attcctttaa ttgcaagaat catttgcatc gccgatgttt
     2101 acgacgcatt gacggaagac cggccgtacc gcagggcctg gagccgaaca gaggtccggc
     2161 agttttttga agagcagaag ggaaaaatgt tcgatgcgga gcttgtggat gttttcctta
     2221 cccttccgga tgttcagtaa agagatgatc aaaacagaat aatttatgtt gcggcggagg
     2281 ggctgctcga cggaaatcct gttaatgcgc atatggggaa aacattgaaa aatgtttccc
     2341 ccatagtgat acttgaacaa agtggcaaag gtttattttt ctttttcaaa ttttgattta
     2401 tccgcgccgc agatggggca ggtccaggaa tccggtaaat cttcaaattt tgttccggcc
     2461 gcaatgccgt tatccggatc gcctttgacc ggatcataaa catagccgca gacgctgcaa
     2521 atataagacg ctacggtctg aggagccgcg acggccggtt cttctttgga atatgtcgga
     2581 gcggttttcg gcgatttacc tcctttaacc tgctgataat aagcgtaggt catgggtacg
     2641 gcgtcgctga tcatgtcgca gtcgacaata ttgcccatga aaatggtatg agttccgcaa
     2701 tccatttctt tttgcacgtc gacttcgatg taggatacgc tattgtccag aacgatcggc
     2761 acctgggtca cgccggtttt tacctttaca ttctgtaatt tattgacgtc acggccgctt
     2821 ttaaatccga acagaccgat cagcgtcatc ggcgccgcct cggacaagat cgaaacgaca
     2881 aatttccggc tttcttttat caatgcatgc gtgtagtttt ccttattgat gctgatggcc
     2941 acggttgccg ggctggaggt aacctgaaac atggaattgg cgatctgacc gttgaattta
     3001 ccgtcctgac cggacgtgac gatgtaaagg ccgtagctga ttttgtgaag agcggatctg
     3061 ttcatgaatg ttctccttgt gcaacgttat tcataaattt tatagcctaa ttatgcattt
     3121 cgtgccattg aaatgtcaaa aagattttgc atccattttt cttttgacaa aagaatcctt
     3181 tttcgatagt ggatgcaaaa tagaattaaa ataaaatctt acgttttttg ttgttgacct
     3241 ttcagcaaag cgggaaacca ttatgaaata tcaggaattt ttaaagagag cccggtttga
     3301 ttttgaagaa attctggcgt ttgcttacgg caagcttgtc gacgatccgc cggaacattt
     3361 tgacgcgaaa tttccggcgc cgccttttct gatggtggat cgcatattgt ccattgaaag
     3421 tgacggcaag aaaggtaaaa taattgcgga acaggatatc agaccggacg cctggtattt
     3481 tcagtgccat ttccagggcg atccggtaca gcccggatgt ctcggggtgg acggattatg
     3541 gcaattgctg ggttttttct gcgtttggag gggtgcactg ggaacaggca gggcattggg
     3601 gtgcggcgat gttgctttca acggtcagat tcgccctttt aacaaatgtg ttcgctatga
     3661 agttgatgtg cgtcgttatt cgatgctgaa ggaatccggc gcaagcatcg tcatcggcga
     3721 tggacgcgtt tatgtggatc atgaattgat ttacactgtc ggacaggccc gtgtcggtgt
     3781 tttcaaggat attgcctaca aagattaccc gcgccgttca aaatattcga tcggcggaat
     3841 catggaaagg taatccgtct gatcatcaag gctgttcttc cggttctgct gaaaaatatc
     3901 attaaaatat tgatcgttgg ttttttaggg aaatattttg aatatcgtta aaaaaacggc
     3961 ggcctatttt gcccgaaaag aattctgccg gaacgccatt aacgaatgcg cggatttgag
     4021 tcttctgaaa gagaaacaca cgctgcgcgt gaccatcgga atcgttctga ttgttttaag
     4081 ttacattgtt gtccttccgt cgtttttcat ggtgggggtt atggctgcca ggttgcgaaa
     4141 accgatggtt ggtgttatag gcattcctct ggcttacggt ttttcgtggt tgatcatgat
     4201 gatggggatg tatcttaccg gtcccgaata tgcgaaaacg ctcggaaaat atgtggtgag
     4261 ggtggtgttg gagaaaattc tcggagatga ggcaaaaaaa atcgcctcca agccgaataa
     4321 aaatatcgac aagacaggag aaccgaatga gtaacgccgc agaaatgata agagaactga
     4381 aaatcaaaat tatcgagaca ctggggttga ttgatgtcgg ccccgatgac atccaggacg
     4441 atgaacggtt ggtcggcggt gatctgggaa tcgattccat cgatgtgctg gaactggtca
     4501 tgatgatcga aaaggattat ggcgtcaaga tcgatagcaa gaaactgggg gtaaaggttt
     4561 tcgcttccgt cagcgcgctg gccgatcata tcctcaatca tcagaaaaaa acggtttaat
     4621 gtcattgtgc gcaaaacaac cactattcct atgatgaacg tgctgttgat ttccgccaat
     4681 actctgaaaa acccgtatcc ggtgtatccg ctggggctgg attatgtagc cggttgcctg
     4741 gattaccgct accggacaaa aattgtcgat ctgaacgttt ttccccatcc ggaaacactg
     4801 gaagcaatga tcagggaatt ttctcccgat gttgtgggaa tttccatcag aaacatcgat
     4861 aataccgatg cgatcaactg tcgcagtttt cttaccgaat acgagacgtt agtcggccag
     4921 atcagaaaaa attccagggc taaaatcatt ctgggcggca gcggcttcac gatttttccc
     4981 cgcgagttca tgcaggcgct cgatgccgat tacggaatcg tgggggaagg cgaaagactt
     5041 gcactgcttc tggacgcgtg ggagaaaaat gaggatgtga acaaaattcc cggtgtcctg
     5101 accagcgtta aaacggatat tgtctacgaa ccttttggcg gaagactcaa cagacatttt
     5161 gatcccgaag aaactcacac gcgcttttat cttgcgtacg ggggaatgct caatctccag
     5221 acaaaacggg gctgtccctt ccgttgcggc tactgtacct atccccatat cgagggcggt
     5281 aagatgcgat gctatgatcc ggaggagatc gcccgcagcg cccgcgcatt acaggatgcc
     5341 ggtgccaaat atatttttat taccgattcc gccttcaatg ccagctacga acacagcgct
     5401 caggtggcga aggcttttac gaaggcgggc gtgtcgatcc cctggggagg tttttttgcc
     5461 tccaccgttc ctccagccga ttacttcaag cgtctggcgg atgccggttt gacccacgtg
     5521 gaattcggca cggaatcttt atccgatcag gtgctggaga atctgcgtaa acccttcacg
     5581 tcggaaaatg ttttcacggc gcaccgcgaa gcgctggacg ccggattata tgtggcgcat
     5641 tattttctgc tgggcggtcc cggcgagaac aaagaaacgc tgcaggcaac tctgatggga
     5701 atcgataagc tcgaaagagc ggtcttcttt ttcttctgcg gcattcgcat ctacccgcac
     5761 acggcgcttt atgatgttgc cgtgcgcgaa ggccagatca aagcggggga aaatattctg
     5821 ccgcccgtgt tttaccgttc tcagggaatc cgtgttgaag agattatgaa gacggtggaa
     5881 aatcatgcca acggccggtt gaactggctg atcggcgccg gcgaagccaa ggccacacgc
     5941 atcctgccga aactttacag acgcggattc accggaccgt tatgggagta tctgattcag
     6001 taaaaatata acttcgtaaa tatttaaaaa cgtccggacc gtaactcaat gctgcttttt
     6061 ttgtcctgcc cgcagaggcg ggcgaagtgc ccttgagagt gactccggaa atatgagagg
     6121 ctgaaacatc agaattgcag ggactgttcc cctgacagtt caaaacggcg cattcacgga
     6181 atgcaccata cccgtgtagc ggttatccat gaaaaagaaa ttgtcatctg caaaccctgc
     6241 tgaaatgacc ggagtcattc ttttgcttgc cgcggctgtg atttttttct ttcatccact
     6301 ggccgcggca gccgtcgctc ttttttatat tgttctgtgc gttgccgctt cgtttttccc
     6361 gcaaagcagt ttctacctgc ctgtcatcag ccgggggaga accggcagga atgtcgttgc
     6421 tctgacgttc gacgacggac cggccgaacc gacgacaaga cggattctgg atctgctgga
     6481 tcgctattcg gtcaaagcga ctttttttgt ttccggcgtc aatgctttaa agcatccgga
     6541 catcgtcagg gaaatcatcc gacgcgggca caccgtcggc aatcattcat tccatcacaa
     6601 tccgtttttg atgttgggca gttatcgtta tctttataac gaagtatcca aagctcagga
     6661 catcctgaaa gacaggggtg tcaatacgtt gattttccgg ccgcctgtgg gcatcatcag
     6721 ccctaaactg ccgtccgtcc tggataaact gggaatgttt tgcgtgacat tcagttgccg
     6781 aggcggtgat ttcggcaacc gtcgcattag aaatttaagt tcccggatat tacacagcgt
     6841 caaagcagac gacattttat tgctgcacga tgcattgccg cgcggtgggg gaaaccaggc
     6901 tgttttattg gtcgaaatcg aaaagatact gcgggggcta aaaaaacagg ggttaaacgt
     6961 cgttcccctc gccgatttaa tcggcaggca cgtatccata atgatgtaag atcgcgtcat
     7021 ctataattaa ttgttgatct tgtaaaaaat ttttgattga aaaagcaaac aatgatatgc
     7081 agttatactt tttggccccg aacgtatgcc gggcatttgc caatgaggta tattgattga
     7141 aaaatcaatg gaatttgtta aaagaaacaa gagaagctga ttctaatgat atcgaggcct
     7201 tagccgatgt cccggcagac tccccctggt tttcaggtca ttttccgggt gagccgattc
     7261 ttccgggcat tgctctggtt tatgcggtaa agcaggccat tcaccgggaa gcgcaaaaga
     7321 aaggcgagcg gcttgaattg catactttga agagaatccg gtttatgcaa ccggtaaggc
     7381 cgggtgatca gttatcgttg aatatccgtg cggcagaggc ggatggagaa cgcttgtttc
     7441 attttaaaat tttttgcaga gatacaatgg tcagcagcgg aatgattgct gccaaaaaaa
     7501 tgaagtaata aaaataaata taaatatgca tggtaaggag ggaagtgatg cctgcaagtc
     7561 aagatgttaa aggcattata cggcgtgtag ccgaaatcat tattgatgag ttgaagctgg
     7621 aagaagcgac cgcggatacc tttgatccgg atatggatct ggtggatgaa ctgggtattg
     7681 acagcatgga cctggccacg atcgcactgg ttcttcagga tgaatacaga attaccatag
     7741 acgaggatga ttatccccag ttgaaaacca ttcgtctgat ttcagaatat attaaagaaa
     7801 ggcttccggt cagacattaa ttgacagaat gcgagatggc caatcaggta aacaacaagg
     7861 taaatacgga gtccgtcaaa ccgaaatttt ccgagcttct agcccgtccc gaagtcaaag
     7921 cgcgctggga gaaggtgaga aagtattttt ttctgcgcga atcgacctac gatatgacga
     7981 accgctgtaa tatccgctgt gacggctgct attattatga aggcgaaaag caattcgcca
     8041 aagaggtcgg cgatgtggag gcctggcgcg cattgatgag agcggaaaaa gcacgcggta
     8101 taacctacgt cgttctggcg ggcgcggaac cttcgcttgt tccggaattg ctggaggtct
     8161 gttatcagga aatgccgttg ggcagcattg ccaccaatgg cttcagaaag attccaccgt
     8221 ccgtcggtta taaactgcac atctccgtct ggggcagtga tgaaaccagc ctgaaaatca
     8281 gaaaggccaa aaaccttctg aaaaaacaga ttgaaaatta cgaaaacgat ccgcgcgcgg
     8341 tttttgtcta tacatttacg aggaataata ttgatgaggt taacgaagta acggaagagc
     8401 tggtcgccca taactgcaaa ttgacgttta atgtgttttc tgcgccggtc ggatatgccg
     8461 gaaatctccg gcatgatgat gcatcgcttg agcaaacgag acagaccatg attgagcttt
     8521 taaatcaata tccgcgcaat gttctttttt ccgtttacaa tgccatagcg catacgcatc
     8581 gaaaaggcct gcacgatctt tacagctgtt cctatccccg tcagaatccg tcgcaggata
     8641 tcgggctggg cagatctttc aggcagtaca gaacggacct gaactgggac cggtcggtgg
     8701 cctgctgcgt accggatacg gattgcgcgg actgccgcca ttacgctgca ggctccgcag
     8761 tggtgacggc gcgactctac cgccacgtga cgaatctggc cacattcaaa tcctggctgg
     8821 attatgtcga tacgtatctg gcggtctggg tgatgaacta tgagaagggt gagaatctct
     8881 gtaatgaaat catagaaccg ccgcgcgcgg cggttgaaaa gaaacaaaac gtgccattgc
     8941 acccgaaatg acatccggat ggtatgtaaa gtcgcaatga tatgatgtag gatagtgaag
     9001 atgaaaacag tcagttccct gctggacaaa gaatggcacg atcgatacag aaggatttcc
     9061 aacctgaata tccgcagttc catctatgat gtgaccaacc ggtgcaattt acgctgcaag
     9121 ggttgttttt tcttttcttc gggcgagcat caggcggcgg tcgaag
//
LOCUS       DAOOLB010000078         8996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_9043_length_8996_cov_36.056817, whole genome shotgun sequence.
ACCESSION   DAOOLB010000078 DAOOLB010000000
VERSION     DAOOLB010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8996
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9043_length_8996_cov_36.056817"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(301..>2271)
                     /locus_tag="PLF77_08910"
     CDS             complement(301..>2271)
                     /locus_tag="PLF77_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09011.1"
                     /translation="RSLPDSTASLSLTGKHVFPIPSHMAGKSISQLQNASGFLIQFLR
                     RKSDIAALILIAAVIALPSISPLSSGSKVNFNDDFFYYAGMHESVRQAVLEHHTFPTR
                     SLWIGGGYPSLGNPEDPALNPLTLITIVFGSVMALKIITFMALLVGGLSSYLLARHIL
                     GYTKWGSLFCGLVFGLSLFVPLRVYDGNTNEVYVAFLPLCMLLIGLACRGRKTAVFIL
                     AFVFYTMLSDGKLIALMIFLYLGILCLLDIIPSFNIFATKNLNKMNIKPLKVLFLALT
                     ITFFVGMLRILPVLDMIETMGGLQSNFLWFKPITYSPSGVVTLQELWHNLIGYKDIIN
                     IATIGLIPVLLALSVLFIFPPKAFPWAVTIFLFTWLMLAYNAPVDLSKLLWHLPVFNI
                     ITKHKYYAFPIVFSLIIVAGQFFWLLSKIRSKWLEHALAAALILLSVWFLYPRFDAIQ
                     KNTYTYDIPEEFLVRQNEFYNIQGLGIPRFRAKPLNSVIYTNVIRNVGIIDSVTHYGE
                     KAVPKYFVDAEGTLIPNPGYKGEAHFADPANTAKATFRPNSIMVQVNLRKPDMLVINQ
                     NYERNWHSEHGRIFNKGGLIALRLDKPGVYDITLRYFFRSFFVGLFLSIVSLIALISI
                     CWSYKTGRLIKWSHDAPIHIRWMPRFILWLIN"
     assembly_gap    2272..2371
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2620..4089)
                     /locus_tag="PLF77_08915"
     CDS             complement(2620..4089)
                     /locus_tag="PLF77_08915"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC09012.1"
                     /translation="MRKILFIYPRLGYMDAQRSKPAPPLGILAAVSFLKNTYEIIILD
                     QRLFASDDSFYAKLYNVLQNQPLYAGLSVYSGGMIKFALEISRYIKEQSSVPVVWGGI
                     HPSLLPEQTLENQYIDYVIQGEGELTLPEFSDRLVQYGQNMPPVRGVWIKKQGKIAYG
                     GDRDLIDLQAAPVIPYDLVDFNNYVQYYNGHKYAYYQASRGCPRHCRYCYNHVFNKGK
                     FRAKSADKIISEIKTLAERYASFTGIFFVDDNIFAMGKDYVMNLGKGLGELGLRWAVQ
                     GSDIIALKDYTESDFKRLEMCGLTRLTIGVESASEKIRTLIGKQGNPADIEQVMARLS
                     GTKILIWCSYVISLPGETIEDLRASIKLIFRLQHINPNVRNSPFYMYIPFSGTPLYEQ
                     YKDIFPGPESLEEWGQVGWEREHTNSFADYLKDTHFFQSLFLTSLLDDDKVSDFSKNK
                     LLVFLAKCYRPVARWRLKNLFFKFNIELSMFKKFFPDIF"
     gene            complement(4097..5194)
                     /locus_tag="PLF77_08920"
     CDS             complement(4097..5194)
                     /locus_tag="PLF77_08920"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC09013.1"
                     /translation="MNWNSLFDLSNLYGLACNIWPRFPYNIPQLQTALKPFLYLFNIT
                     NQCNLNCYYCCQKTSLTRNRPELSFEDISGIIKKLPRYSVIVFSGGEPLCRDDFTKLL
                     KETRRLKRKTALLTNGLLLNDDIICEMIQNRLLNIGIAIDGDKYYYEKIKGRGNYAVV
                     MDNVDRLAYHKKKAKSSFPAFDWKVTVFPENVSRLPLLYQQAADHGADSFTVSFPKKN
                     DFQFNDCLHDIAVLDCEPVSNGNYRFPENIEDIYKELFSIARTSRTRLRTYPQMKQAE
                     DLKNYFETSAIQKRYSACRMPWSGMVISSTGEAYPCLSVRIGDLKKQSLAQVLTSPEN
                     IRFRNQLKKRSLFAICDGCCYARLKPVLKRE"
     assembly_gap    5379..5478
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5479..6884)
                     /locus_tag="PLF77_08925"
     CDS             complement(<5479..6884)
                     /locus_tag="PLF77_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC09014.1"
                     /translation="MKRPKILLFKSHSIVSKVTGVTPPLGLLYIAAALRERLGADVRV
                     IVAMLENDPMNVMVSTLRHFRPDVVGISALTAEAFFAHKAAAAVKAEAPSVPVIMGGP
                     HASCDPELVLQDSCVDAVVMGEGEDTFTELVSVTMSEKSVWQNPDILQKIDGLAFRVD
                     GKMKLSKPRAPINDLDALPFPAWDLIDYKKYWALPKMATDGVRPYLTMFTSRGCPYNC
                     IYCHQLFGKSFRSRSPESVVEETARLMRMGAESIEILDDISNFNPERFDRMLELMLEK
                     NLHPVLSFPNALRADLIRKESADLLKCVGTGEISVAIETASDRLQKLLGKNLSLEKAK
                     RTIDWFADRRIFTRGFFMLGFPTETKDEMLATIRFAHASRLHMALFFTPNPFRNTALY
                     DLFVDTGKLPSTSTSIDYEYYGAPFNGSDVSDRRYRMLYRWAYYGFYFNPVRLWRIAQ
                     DRPTLADIPASAWTRLNNV"
     gene            complement(7012..>8996)
                     /locus_tag="PLF77_08930"
     CDS             complement(7012..>8996)
                     /locus_tag="PLF77_08930"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC09015.1"
                     /translation="VFSQVGQFDFITFDDPVYVVDNPSIQSGFTMDGVIWAFGTIHAE
                     FWHPLTWLSLMLDYQLFGLNAGGYHITNLMLHILSTLMLFWLLHRMTGAVWRSAFVAA
                     LFAIHPLHVETVVWIAKRKDVLSAFFWMLTLCLYVYYTEKPMVKRYLFVFLSFACALM
                     SKPMAVTLPVIMIVLDYWPLKRFASKKENPVFWQVKEKLRFFMLSAVFSLITIFAQYD
                     KYIKHAETSLEARLLNAPVSFITYLAKTFWPHDLAVFYPFSYQLPAWQTAGSIALILA
                     ISVAVILAAKRLPYLLVGWLWYVLAVLPVLDIFRSSARAMSDNYTYLPAVGIYVMAAW
                     GIPDVFCQKQIRRKILWPAGIAVAAMLSVLAWQQCSYWQNSFKLFNHALHVTKDNYLA
                     HNQRGNVYSRSQQHALAIADFNEALRIKPDYAFAFNNRGNTYLQLGRYQQAISDYSEA
                     IRLNPDYARAYYNRGFTYGIHMGRYQRAIDDLNDAIRREPDAPDIHLTRGIIYSNLGG
                     QELACLDYQKACDLGNCKLLDMALGKGECLAADLQPHDSGYYHNSGISYFKRGQYRIA
                     LGYFTHAIHFKKDFANAYHNRGTTYSELGRYQHAVADFNESIRLNPGYPDAYKNRGIA
                     YLLQGEKEQGCSDFKKACALGDCKLLELAESKGDCR"
BASE COUNT         2425 a         1975 c         2105 g         2291 t
ORIGIN      
        1 cagccaggtc aaaggatgcc agaattccgc gtaggtagtc gtcaatgccc agcggatact
       61 cgccagagtg atgccggact ggacatgatg gttgtcggta atataaacat tatcatcaaa
      121 attgatgaag tcaaattgtc ccacctgacc ataaacagcc agtgagacaa ccgtcaaaaa
      181 aatatatata aaaacctttt gtctctgaag accggtatcc atcgtattta atatatcgga
      241 ttatttttgc cgtacttgta ctacatgttc aatcagaatt cagtagtttt ttagtaatgc
      301 ttaatttatg agccaaagga tgaatcgagg catccatctt atatggatgg gggcatcatg
      361 tgaccacttg attaatcttc cggttttgta gctccaacag atagaaatca atgctattag
      421 gctgacaata cttaagaaca aaccgacgaa gaaactgcga aagaagtaac gaagggtaat
      481 gtcgtaaacg ccgggtttgt ccagacgcaa tgcaatcaaa ccacccttat tgaaaatcct
      541 tccgtgctcc gaatgccaat tcctttcata gttttgattg atcacaagca tgtccggttt
      601 tcgcaggttc acctgaacca tgatggaatt tggccgaaaa gtggctttgg ctgtgtttgc
      661 ggggtcggca aagtgggctt ctcctttgta tccgggattg gggataagtg tgccttccgc
      721 atcaacgaag tattttggga cggctttttc gccataatgt gtgacgctgt caatgatgcc
      781 gacattacga atgacgtttg tatatataac ggaattcagc ggttttgccc gaaagcgtgg
      841 aattcccagg ccctgtatat tataaaactc attttgcctg acgagaaatt cttccgggat
      901 atcgtaagta tatgtgtttt tctgaatggc atcgaaccgc ggatacaaaa accatacgct
      961 caaaaggatt aatgcggcag ccaatgcatg ttccagccac tttgaacgta tttttgacaa
     1021 taaccagaag aattgtccgg cgacaataat caaagagaag actattggaa aagcgtaata
     1081 cttatgttta gtgattatat taaacacggg gaggtgccaa agcagctttg acaggtccac
     1141 gggagcattg taggccagca tcaaccatgt aaataaaaaa atggtaacgg cccagggaaa
     1201 tgcctttggg ggaaatataa acaaaacgct taaagcaaga agcacgggga ttaatccgat
     1261 cgttgcgatg ttaataatat ctttatagcc gattaaatta tgccataact cctgcaacgt
     1321 aacaaccccg ctggggctgt aagtgatggg cttgaaccaa agaaagttgc tttgtaaccc
     1381 tcccattgtt tctatcatat ccaatacggg taaaatacgc agcataccga caaaaaaggt
     1441 tatcgtcagc gccaggaaaa gcacttttaa aggtttaatg ttcatcttat ttaaattttt
     1501 tgtcgcaaaa atgttaaatg agggaatgat atccagcaaa catagaattc cgagataaag
     1561 aaaaatcatc aaagcgataa gcttgccgtc cgaaagcatg gtataaaaaa caaaagccag
     1621 aatgaagacg gctgtctttc ttccgcggca tgccagtccg ataagcagca tgcataacgg
     1681 gagaaaagcg acgtaaacct cgttagtatt gccgtcatat acccggaggg ggacaaacaa
     1741 gcttaaacca aaaaccagtc cgcagaaaag cgatccccac ttggtgtagc cgagaatatg
     1801 tcttgccagt aaataactgc tcaacccccc gacgagcaac gccataaaag taatgatctt
     1861 tagagccata acagatccaa aaacaatggt gatcaaagtc aacgggttta gtgcagggtc
     1921 ttccgggttg ccgagagaag gataaccgcc tcctatccag agcgagcgag tcggaaaagt
     1981 atgatgttcg aggacagctt gtctgaccga ttcgtgcatt ccggcataat aaaagaaatc
     2041 atcattaaag ttaactttac tccccgatga aagcggtgag atcgacggca gtgcgatgac
     2101 cgccgcgatc agaatgaggg cggcaatatc tgattttctt cttaaaaatt gtatcagaaa
     2161 accagatgcg ttctggagtt gtgatataga ttttccggcc atatgagacg gtataggaaa
     2221 aacatgtttg cctgtcaatg ataaactcgc tgttgaatcc ggcagcgagc gnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaaaaacat gtttgcctgt caatgataaa
     2401 ctcgctgttg aatccggcag cgagcgcatc caacgatacc tgcggcgggg tcggcgagcg
     2461 acattaggta agaacgtcat tccctatgga tcgaagactt tccagcgcgc tgtgaaaagt
     2521 ttttatttat tacattcggt ttgccggatg atgtttgccg ggatttttgt cttgaacaaa
     2581 tggaggagcg tcggggatga tcaaaccagg caattcatat cagaaaatat ctgggaaaaa
     2641 ttttttaaac atcgataatt ctatattgaa tttgaaaaac aggttcttta aacgccagcg
     2701 ggcaacagga cgataacact ttgccagaaa aaccaaaagt ttgtttttgg aaaagtcaga
     2761 aactttatcg tcatcgagca ggctggttaa aaacagcgac tgaaaaaaat gcgtatcttt
     2821 tagataatcg gcgaagctgt ttgtgtgttc tctttcccat cctacctgac cccattcttc
     2881 cagtgattcg ggaccgggga aaatatcctt atactgttca tacagcggtg ttccggaaaa
     2941 cgggatgtac atatagaagg ggctgttgcg gacgtttggg ttgatgtgct gcagccggaa
     3001 aatcagtttg attgatgcgc ggagatcctc gatggtttct ccgggcagac tgatcacata
     3061 gctgcaccag atcagaattt ttgtcccgct caatctggcc atgacctgtt cgatatcggc
     3121 cgggttcccc tgtttgccga ttaacgtccg aatcttttcc gacgccgatt caaccccaat
     3181 ggtgagcctt gtcaaaccgc acatttcaag acgtttgaag tcgctttcgg tgtagtcttt
     3241 cagcgcaatg atatcgcttc cctgaaccgc ccagcgcagg ccaagctcgc ccagcccctt
     3301 tccgagattc atcacatagt ccttgcccat tgcgaatatg ttgtcgtcaa caaaaaagat
     3361 tcccgtgaaa gaagcgtatc tctcagccag agttttgatt tctgaaatga ttttatccgc
     3421 agattttgca cggaatttcc ctttgttgaa aacatgatta tagcagtacc ggcaatgtcg
     3481 cggacacccc cgcgatgcct ggtaataagc gtatttgtga ccgttgtaat attgcacgta
     3541 attgttgaaa tccaccagat cgtatggaat gacaggggcg gcctgtaaat ctattaaatc
     3601 tctgtctcct ccgtaagcaa tctttccctg ctttttgatc cagacgcctc tgacgggcgg
     3661 catattctgg ccgtattgga caagcctgtc ggaaaattcg ggaagggtga gttctccttc
     3721 tccctggatg acataatcta tatactgatt ttccagtgtt tgttccggta ataaagacgg
     3781 gtggatgcct ccccagacaa cggggacgga tgattgctct ttgatatacc ggctgatttc
     3841 cagagcaaat ttaatcatcc cgccgctgta aacggacagt ccggcataga gaggctgatt
     3901 ttgcagcaca ttgtaaagtt ttgcgtaaaa agagtcgtcg gaagcaaaaa gacgctgatc
     3961 cagaatgatt atctcgtagg tatttttcaa aaacgacacg gcggcgagga ttcccagcgg
     4021 cggcgcaggt ttgctgcgtt gggcatccat gtatcctaat cgagggtaga taaaaagtat
     4081 tttacgcacc gacagctcac tctcttttca aaaccggttt taaccttgca taacagcaac
     4141 cgtcgcagat tgcaaagaga cttctttttt ttaattggtt tctaaaccga atattttccg
     4201 gcgacgtaag aacctgggca agcgattgtt tctttaaatc ccctattctg acggacaagc
     4261 aggggtaggc ttctccggtg gaacttatga ccattcctga ccacggcata cggcaagcgg
     4321 aatatctttt ttggattgca gacgtttcaa aatagttttt cagatcttcc gcctgtttca
     4381 tctgcggata tgttctgagt ctggtccggg aagttctggc tatggagaat aattccttat
     4441 aaatgtcttc gatgttttcc gggaagcggt agttcccgtt tgatacgggt tcgcaatcaa
     4501 gcactgcgat gtcgtgcaga cagtcgttga actgaaaatc attttttttg gggaaggaca
     4561 ctgtgaatga atcagcgcca tgatcggcag cctgctggta aagaagcggg aggcggctga
     4621 cattttcggg aaaaaccgtt actttccagt caaatgccgg aaacgaactt ttggcttttt
     4681 tcttgtgata ggccagacgg tctacattat ccatcacaac agcataattg ccccgcccct
     4741 ttattttttc ataatagtat ttatcaccgt caatcgcgat gccgatattc agtaatctgt
     4801 tttgaatcat ttcgcaaatg atatcgtcgt tcaacagtaa tccatttgtt aaaagcgctg
     4861 tttttctctt tagacggcgg gtttctttca atagcttagt gaaatcgtcc cgacaaagag
     4921 gttctcctcc ggagaacacg ataaccgagt atcgcggtaa tttcttaata atgccggata
     4981 tgtcttcaaa ggatagttcg ggccgattcc gggtcaggct cgtcttctga cagcagtagt
     5041 aacagtttaa attgcattga ttggtgatat taaaaagata gaggaatggt tttaatgcag
     5101 tttgaagctg cgggatgtta tacgggaaac gcggccagat gttgcacgct aatccatata
     5161 ggtttgataa atcgaacaaa gaattccaat tcataacata actttttttc gaaggataaa
     5221 attattaaac attagattca atttatatac gatgaaaaaa atctctcatc gtactgctta
     5281 caccagggat gtctcttctt ctttgagtcg acggaaagat acgacgttat tcaggcgcgt
     5341 ccacgcgctt gcaggaatat cggcaagggt cggcctgtnn nnnnnnnnnn nnnnnnnnnn
     5401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnac gttattcagg cgcgtccacg cgcttgcagg aatatcggca
     5521 agggtcggcc tgtcttgcgc tatacgccag aggcgcaccg gattgaaata gaaaccgtaa
     5581 tacgcccagc ggtaaagcat ccgatagcgc cggtctgaaa catcgcttcc gttgaaaggc
     5641 gctccgtaat attcatagtc aatgcttgtg gacgttgaag ggagtttgcc cgtatcgacg
     5701 aaaagatcat agagcgctgt attgcggaaa ggattgggcg taaaaaacag tgccatgtgc
     5761 aatctggagg catgcgcaaa acggattgtg gcaagcattt catctttcgt ttccgtcgga
     5821 aagccgagca tgaagaaacc gcgagtgaaa atgcgccggt ccgcaaacca gtcaatggtg
     5881 cgcttcgctt tttccagaga gagatttttg cccagcagtt tctgcagcct atccgatgcg
     5941 gtttcaatcg ctacggaaat ctccccggtg ccgacacatt tcagcaggtc ggcggattct
     6001 ttacgaatta aatcggcgcg caatgcattg gggaaactca acacgggatg cagatttttt
     6061 tccagcatta attccagcat tctgtcaaaa cgttccgggt taaaattgga tatatcgtcg
     6121 agaatctcta tgctttccgc gcccattctc atcagccgtg ctgtttcttc aacgacgctt
     6181 tccggtgacc ggctgcggaa ggacttgccg aaaagttgat ggcaataaat acaattatag
     6241 ggacaaccgc gcgacgtgaa catcgtcaga taaggcctca caccatccgt tgccattttg
     6301 gggagggccc agtatttttt gtaatcgatc aggtcccagg cgggaaaagg cagagcgtcg
     6361 agatcgttta tcggcgcgcg cggttttgaa agcttcatct ttccgtcaac gcgaaaagcg
     6421 agaccgtcaa tcttctgcaa tatgtccgga ttttgccaga cggatttttc agacatggtg
     6481 acactgacca gttcagtgaa tgtgtcttcc ccttcgccca tcacaacagc atccacgcag
     6541 gaatcctgca aaacaagttc cggatcgcag gatgcatgag gtccgcccat gatgaccgga
     6601 acggacggtg cttccgcctt gacggcagcg gcagctttgt gcgcgaaaaa agcctctgcg
     6661 gtcaaagcgc ttattcccac gacatcagga cgaaagtgtc tcaacgtcga taccatcacg
     6721 ttcatcgggt cgttctcaag catagcgacg atcacgcgca catccgctcc cagccgttcc
     6781 cgcagagcgg cggcgatata gagtaatccc agcggcggcg tcacgcctgt gacttttgaa
     6841 acaatgctgt ggcttttgaa aagaagtatt ttaggtcgtt tcatcgttca tcttccaaga
     6901 caacccatcg caaacttcga tgaagtttcc tgaaatgtgt atgacgaatg taaaattatc
     6961 tcaaccattt tttaaattga cgattttatt tcgagatatg aataaaattc atcaacggca
     7021 gtcccctttg ctttcggcaa gttccagtaa tttacagtct cccagcgcac aggctttttt
     7081 aaaatcagaa cacccctgtt ctttctcgcc ctgtaataaa taagcaatgc ctctgttttt
     7141 ataagcatcg ggataacccg ggttaaggcg gatggattcg ttaaagtctg caacggcatg
     7201 ttgatagcgg ccgagttcgc tgtaggtcgt tccccggttg tgataggcat tagcgaaatc
     7261 ctttttaaag tgaatggcgt gagtaaagta tcctaacgct atccgatact gtccacgctt
     7321 gaaataagag atgccgctgt tgtgatagta tccgctgtcg tgtggctgaa gatccgctgc
     7381 cagacattct cctttgccca gtgccatatc cagaagtttg cagttgccca ggtcgcatgc
     7441 cttttgataa tcaaggcatg cgagttcttg gccgcctaaa ttcgaataaa tgatgcctcg
     7501 ggtcagatgg atatcgggcg cgtcaggttc tcttcgaatc gcgtcgttca aatcatcaat
     7561 ggcgcgttgg taccggccca tatggatacc gtaagtaaag cctctattgt aataggctct
     7621 ggcataatca ggattcaggc gtatggcctc gctgtagtcg cttatggcct gttgatagcg
     7681 gccgagttgg agatacgtat tccccctgtt gttgaaggca aaagcatagt ccggttttat
     7741 gcgcagggcc tcgttgaaat ccgcaatggc cagggcatgc tgttgtgacc ggctgtatac
     7801 attgcctctc tggttatgcg cgagataatt atcttttgtg acatgcaaag catgattgaa
     7861 aagcttgaaa ctgttttgcc agtaactgca ttgttgccag gcaagaaccg acagcattgc
     7921 cgcaacggcg attccggccg gccataaaat ctttctccgg atttgttttt ggcaaaacac
     7981 atccggaatg ccccaggccg ccatcacgta aatgcctaca gcgggcagat acgtgtaatt
     8041 atcggacatg gcccgcgcac tggaccggaa gatatcaagc accggcaaaa cagcaaggac
     8101 ataccataac catccgacaa gcaaatacgg cagacgtttt gccgcgagga tgacggcaac
     8161 gctgatggcc aggatcagcg ctattgatcc tgcggtctgc caggcgggca actgatagga
     8221 aaaaggatag aaaacagcca gatcatgagg ccagaacgtt tttgccagat aggttatgaa
     8281 agaaaccggc gcgttgagca ggcgggcctc aagagatgtc tcggcatgtt ttatgtattt
     8341 gtcgtactgg gcgaagattg tgatgaggga aaaaacggcg gataacatga aaaaacgcaa
     8401 tttttccttg acctgccaga aaacgggatt ttcttttttc gaggcaaacc gtttcagcgg
     8461 ccagtaatcc aaaactatca tgatcaccgg cagggtaacc gccatgggtt tgctcatcag
     8521 cgcgcaggca aaggacagga aaacgaatag atagcgtttg accattggct tttccgtgta
     8581 ataaacatac aggcacaagg tcagcatcca gaaaaaagcg ctcaggacat ccttgcgttt
     8641 tgctatccag acgacggttt ccacatgaag cggatgaatg gcgaacagag cggcaacaaa
     8701 agcgcttctc caaacggctc ccgtcatgcg atgcaagagc cagaacagca tgagcgtgct
     8761 caggatgtgc agcatcaggt tggtgatgtg ataccccccg gcgttcaacc cgaagagttg
     8821 gtaatcaagc atgagcgaaa gccaggtcag cggatgccag aattcggcat ggattgtgcc
     8881 gaaggcccag ataacaccgt ccattgtgaa ccccgattga atagacgggt tgtcaacgac
     8941 gtagaccgga tcgtcaaatg ttataaaatc aaattggccg acctgcgaga aaacaa
//
LOCUS       DAOOLB010000079         8749 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_9405_length_8749_cov_45.272602, whole genome shotgun sequence.
ACCESSION   DAOOLB010000079 DAOOLB010000000
VERSION     DAOOLB010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8749
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9405_length_8749_cov_45.272602"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..4517
                     /locus_tag="PLF77_08935"
     CDS             171..4517
                     /locus_tag="PLF77_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HPC09016.1"
                     /translation="MEISFDRSEIDQSIVKRFERQVRLYPDQIAVKDAHRSISFAELN
                     TTANRIAVHLLDKRGDSSEAVALLFEHGLTAIIAILAVLKAGKYYIPLDPSYPRTAIE
                     HMLKDSQAAVILADQTTMDLAGELTAGGITVINADAIDGEADNREPDATIAADAYACI
                     LYTSGSTGLPKGILHTHRTILHHIWSHSRTFGITGKDRQALLLSYSFAASISEIFGAL
                     LNGATLVMNYVKKTGMDHLAKWLQSEKITTFKLPISLFRLFLNTLKGPPRFPDLRLII
                     LGGDKLLRTDVELFRCFFPRDCLLVNRLASTEAFTMTRFFIDQSTALTDQVVPVGYPD
                     PDKEILILDDNAQPAPEGETGEIVVAGRYLSPGYWRLPELNEKKFRTRANHEGQRLFY
                     TGDMGRLRPDGCLEHFGRKDDLIKIRGYRIELSSVEAALNDLKEVREAIVAVQEAGKS
                     IGRKKLVAYLIPAHPAGLSVSQLRIKLAKKLPNYMIPANFVILEKFPLTSSGKIDRLS
                     LPTTEKNRPNLDTPYVAPRTPAEHSIMEIWSEALGLENVGVTDNFFDLGGDSLMLFWV
                     HTHLQDVFDIDIPIIILFKYPTIAALARFIIHRKGLMEGKPPADATAPDVFRPNRPAE
                     PKHAAGSEGSPVRDDIAIIGMAGTFPDAANTDEFWENIKNGVSSVRHYSTQELIDAGV
                     DENLLGDPDYVKAGTYLEDIEKFDAAFFGLRSVEAAILDPQHRLLLETAWHTLEHAGY
                     IPDDFRGRVGIFAGASPSAYLQKHIMPQRHLLPVVNDLRIQLLNNPEFLATRIAYTLN
                     LKGPAINVTTACSTSLVAVHLACRSLLNGECELALAGGVSVETPQKTGYLYQEGWMLS
                     PDGQCRPFDAEARGIVVSNGAGMVLLKRLGEAVRDGDTIHAVIKGSAVNNDGSFKMGF
                     TTPSADGQAAVIEEAQKDAGVHPETITYVETHGTGTSLGDPIEIAALNQAFRRHTWKT
                     GFCAIGSVKSNIGHASRAAGIASLLKTVLSLQHRQIPPSLNFVNQNPEIDFAGSAFFV
                     NTALRPWPGEEGMPRRAGISSFGVGGTNAHLIVEEWQENSVPPVSKSWQLLVLSGKTE
                     TALNTTARNLLHHLKQNRDQELADVAYTLQAGRKAFAHRRMLVCRDREEAIALLASPS
                     PPEEELGRCDFSKPSLVFMFPGEGGQYVNMAADLYQQEPSFRADIDQGAAMFQKSLGL
                     DVRDVLFPQKPDQQQAEQAMAQKETGRAAIFLVQYAVARLLMSWGVYPEALIGSGIGE
                     YTAACLSGVMTLNEMPTVFAHHHNSDAMKNITLQPPVIPVVSGITGTWLTLQEATDMA
                     YWSKPLQETPRFSDGLKTLAMFAHCILMDLGPGYPLCAMAGNNHDLKNTLILSALPAA
                     DETCLARKTLLRAVGRMWLAGARIDWENLHSQEPRKRIPLPLYPYEKKRYWISRVSAG
                     RIPQTGTIRFTE"
     gene            complement(4522..5826)
                     /locus_tag="PLF77_08940"
     CDS             complement(4522..5826)
                     /locus_tag="PLF77_08940"
                     /inference="COORDINATES: protein motif:HMM:NF018513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HPC09017.1"
                     /translation="MTPVTVGVFGLIVMAVLIMIRVPIGCAMGIVGLAGFSILSSPDA
                     AFSMLGMVVYQTVSSYALTVIPLFFLMGQLAGQSGMGVELYRAVHRWTGFLPGGLAMA
                     TVGACAGFSAISGSSLATAATIGAVALPEMKRFRYDDALATGCVAAGGTLGILIPPST
                     VMVVYAILTGQPVGTLFIAGIIPGIILTGLFLLTIFLWSRKRHGISHPDSRFSFAEKW
                     ESLRDTGSVLLTFALVVGGLYGGWFTPTEAAAVGALIVLVIISAKKRLTWKLLAGSLT
                     ETALTTAMIFAILIGANIFGYFMTLSDLPATVTAWVTDTGMNRYATLGIICLVYVVLG
                     CILEGFAIMFLTLPIVFPMVTAMGFDPIWFGVLMILLIEMGLITPPVGINVFIISGIA
                     KNVPMATIFRGIAPFFIAILICVVLLFLFPQLALFLPATMAG"
     gene            complement(5823..6311)
                     /locus_tag="PLF77_08945"
     CDS             complement(5823..6311)
                     /locus_tag="PLF77_08945"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPC09018.1"
                     /translation="MKRVFSCIHKTATGLNGVAAGIIFILMILTAANVLLRKLTGISI
                     TGMTEISELMMIILVFFALAQAEIMDRHVSVSLLTDRLTSGARKYLAVITSLANALFF
                     ALITAAAIVYGSSLRLSGDVSMDLQIPKYPFAYVIAFGAAVLSFVFFKKTLASIKDEK
                     VL"
     gene            complement(6325..7341)
                     /locus_tag="PLF77_08950"
     CDS             complement(6325..7341)
                     /locus_tag="PLF77_08950"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HPC09019.1"
                     /translation="MKKHYLILLMICGVLLTAGPVAAQDAVELKLAHFIPSTHIQHQK
                     TFLPFAQNVEKLSGGKVKIKIYPAGTLGGAVQLPDAVKTGIADIALIFPSMTSGRFPR
                     FSAYDLPFLFDNPQQAIQVAYDLYERWFAEDFKDYKLLWIYCTDMGQLYSVDKPILTM
                     EDFKGMKLRSPSASMSDALKRLGANPVGMPITEMHMALAKHVIDGALTPNTVVADFKL
                     YDQIKHITQTDFYISIMAVVMNKKKYEALSPAAKQAIDNAAGKSWGLHATGVYDDYSR
                     ETVNQLRTSGNVKIHDMAPDERKKIRNQLAGMESDWIRQNSEKGIPAREMMDAIHRAS
                     AKIK"
     gene            7493..7933
                     /locus_tag="PLF77_08955"
     CDS             7493..7933
                     /locus_tag="PLF77_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09020.1"
                     /translation="MMDIEALIPHREPIKVITEILELDDDSGSAAAVVNERWPLCDGK
                     AVCALVLIEAIAQTSALIEGHKRMKKSMGGTKGWLVGIKTAEFRTATVPLGTRITVTV
                     KGLSSFDEYGVIEGVVKAGDDILLSAVLQAIRFNGHGVEQDIRG"
     gene            7944..8672
                     /gene="fabG"
                     /locus_tag="PLF77_08960"
     CDS             7944..8672
                     /gene="fabG"
                     /locus_tag="PLF77_08960"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPC09021.1"
                     /translation="MEEKKTALVTGASRGIGRATAVELAKNGYFVVINYKSNEAAAAE
                     TLVQVEQAGSGGTLCRFDVANAEEVRTALDQIIKTHKRVDVLINNAGITADGLFVMMD
                     EEDWLNVVNTTLNGFYHVTRPVLREMIRKKRGAIVSVSSVSSILPNRGQANYAAAKAG
                     INAATRSLSKEVARFGIRVNVVAPGPVETDMIRDMPMDDIKKIIPMARVGRPEEVAKV
                     ICFLCSDDASYVTGQVIGVNGGMF"
BASE COUNT         2094 a         2282 c         2434 g         1939 t
ORIGIN      
        1 agcacttccc gaaaatcttc attctaaaat ttacgatttt atgttagacc atgtccggtt
       61 ttgttgaccg caaatacggt actttcatgc aggatacttt ttaaaacctc aatttaaccg
      121 attgccttta cgttgggggc aaatcaatta ttacctgttg tggtaagaat atggaaattt
      181 catttgacag aagtgaaatc gatcaatcca ttgtcaaacg ttttgagcgg caggtccggc
      241 tgtatccgga tcagattgcc gtaaaagacg cacaccggtc cataagcttt gccgaactca
      301 acacaaccgc aaaccgcata gccgtgcatc tcctggataa acgcggggac tcgtcggaag
      361 ccgttgcgct tttgtttgaa catggcctca cggcgatcat tgccattctt gctgttctga
      421 aggcgggtaa atattacatc ccgctggacc cgtcgtatcc ccggacggcg attgagcaca
      481 tgttaaagga ctcgcaggcg gcggttattc tggccgacca gaccaccatg gatcttgccg
      541 gggaattgac ggcgggcggg ataacggtga tcaacgccga tgcgatagac ggggaggcgg
      601 acaaccgcga accggacgcg accattgcgg cggatgcata tgcctgcatt ctttacacgt
      661 cgggttccac cggccttccc aagggaattc tgcataccca ccgaaccatc ctccatcata
      721 tctggagtca cagccggacg tttggaatta ccggcaaaga ccggcaggct ctgctgcttt
      781 cctacagttt tgccgcgtcc atttctgaaa tattcggcgc cttgctcaac ggcgcaacgc
      841 tagtcatgaa ttatgtcaaa aagacgggca tggatcatct ggcaaaatgg ctgcaaagtg
      901 aaaaaattac aacgttcaag ctacccattt ctctgttccg gctgtttctc aataccctga
      961 agggtccgcc ccgattcccg gatttgcggt tgatcatcct gggaggcgat aagctgctgc
     1021 ggactgatgt ggaactgttc agatgtttct ttcctagaga ctgtcttctt gtcaaccgtc
     1081 tggcgtcaac cgaagccttc accatgaccc gcttcttcat tgatcagtca acggctttga
     1141 cggatcaggt cgttccggtg gggtatccgg atcccgataa agagattctc attctggatg
     1201 ataacgctca gccggcgccc gaaggcgaaa ccggggagat tgttgtcgcc ggccgttatc
     1261 tttcgccggg gtactggcgt ctcccggaac taaacgaaaa aaaattccgg acacgcgcga
     1321 atcatgaagg acaacggctc ttttataccg gagatatggg gcgcctgcgg ccggacggtt
     1381 gtctggagca cttcggacgc aaggatgatc tgatcaaaat ccgcggctac agaatcgagc
     1441 tctccagtgt ggaagcggcc ttgaacgact tgaaagaagt ccgggaagca atcgtcgcgg
     1501 ttcaggaagc ggggaaaagc atcggcagaa aaaaactggt cgcctatctg attcctgcac
     1561 atccggccgg gttatccgtc agtcaactgc gcatcaagct ggcgaagaaa ttacccaact
     1621 atatgattcc ggcaaacttc gttatcctgg agaagtttcc cctgacatcg tccggcaaaa
     1681 tcgaccgcct gtccctgccg acgacggaaa aaaaccggcc gaacctggac acgccgtatg
     1741 tcgcgcccag aacgccggcg gaacacagca ttatggaaat ctggtctgaa gccctcggcc
     1801 tggaaaacgt cggggtgacc gataattttt tcgatctcgg cggggattcc ctgatgctct
     1861 tctgggtgca cacccacctt caggatgttt ttgatatcga catcccgatc atcatcctgt
     1921 ttaagtatcc gaccatcgcg gcgctggcgc gctttatcat tcaccggaaa ggattgatgg
     1981 agggcaaacc gcccgccgat gcaacggcgc ctgacgtttt ccgtccgaac cgcccggcag
     2041 agccgaaaca tgcggctggt tccgaaggat cgcctgtccg ggacgatatc gccattatcg
     2101 gcatggccgg cacgtttccc gatgccgcaa acacggacga attctgggag aacataaaaa
     2161 acggggtgtc gtccgtgcgc cattattcga cgcaggaact gatcgatgcc ggcgtggatg
     2221 aaaatcttct tggcgatccc gattacgtga aagcgggaac gtatcttgag gacattgaaa
     2281 aattcgatgc ggcgtttttt ggcctccggt ccgtggaagc ggccattctc gatccgcagc
     2341 accggttgct tctggaaacc gcctggcaca cccttgaaca tgcgggatat attccggatg
     2401 atttccgggg ccgggtggga atttttgccg gcgccagtcc cagtgcttat ctgcagaagc
     2461 atattatgcc gcaaagacac ctgctgcccg tcgtgaacga tctgcgcatt cagttattga
     2521 acaatccgga atttctggca acacgcattg cctatacctt gaacctgaaa ggtccggcca
     2581 tcaatgtcac gaccgcctgc tcgacgtctc tggtggcggt gcatctggcc tgccggagtc
     2641 tcttaaacgg agagtgcgaa ctggcgctgg ccggcggcgt gtcggtggaa accccgcaga
     2701 agacgggtta cctttaccag gaaggctgga tgttatcgcc cgacggtcaa tgccgcccct
     2761 ttgatgcgga ggcgcgcggg atcgtggtca gcaatggcgc cggaatggtt ttgctcaaac
     2821 gactgggcga agccgtccgc gacggggata ccatccatgc ggtgatcaaa gggtcggcgg
     2881 tcaacaatga cggatcgttc aagatgggtt ttacgacgcc cagtgcggac ggacaggccg
     2941 ccgtcatcga ggaggcccag aaagatgccg gggtccatcc cgagaccatt acgtatgtag
     3001 aaacgcacgg caccggaaca agccttggcg atcccattga aattgccgct ttaaaccagg
     3061 cctttcgccg ccatacctgg aaaacgggtt tttgcgctat cggttccgtc aaatcaaata
     3121 tcggccatgc cagccgcgcc gccggtatcg ccagcctgct gaaaaccgtt ttatcgctcc
     3181 agcaccggca gattcccccc agcctgaatt ttgtgaatca gaatccggaa attgattttg
     3241 ccggcagcgc cttttttgtc aacaccgctt tgcggccctg gcccggtgag gagggaatgc
     3301 cgagacgggc cgggatcagt tcgttcggcg tgggaggaac aaatgcccat ttgattgtgg
     3361 aggaatggca ggaaaacagc gttcccccgg tatcaaagtc gtggcagctt ctggttttgt
     3421 cgggtaaaac cgaaacagcc ctgaatacga cagcccgcaa tctattgcac cacctgaagc
     3481 aaaaccgcga tcaagaattg gccgatgtcg cttatacgtt gcaggccggg cgcaaggcct
     3541 ttgcgcatcg ccgcatgctg gtttgccggg atcgggaaga ggcgatcgcg cttctggcgt
     3601 caccatcgcc gccggaagag gagttggggc gctgcgattt ttccaagccg agcctggtct
     3661 ttatgtttcc cggcgaaggc ggacagtatg tcaacatggc cgctgatctg tatcagcagg
     3721 aaccctcgtt ccgtgcggat atcgatcagg gcgccgcgat gtttcaaaag tcgttggggc
     3781 tggatgttcg cgacgtcttg tttccgcaaa agcccgatca gcaacaggca gaacaggcca
     3841 tggcgcaaaa agaaaccggc cgcgcggcga ttttcctcgt ccagtacgct gtggcgcgtc
     3901 tgctgatgag ctggggcgtc tatccggagg cgctgatcgg cagcggtatc ggtgaataca
     3961 cggcggcctg cctgtccggg gtcatgacgc tgaatgagat gcccactgtg tttgcacatc
     4021 atcataattc agacgctatg aaaaatatta cccttcagcc gcctgttatt cccgttgttt
     4081 ccgggataac cgggacatgg ctgacgctgc aggaggcgac ggacatggct tactggagca
     4141 aacccttgca ggagacgccc cgtttcagcg acgggctgaa gacattggcg atgtttgccc
     4201 attgtatcct gatggatttg ggaccgggat atccgttgtg cgcaatggcc ggcaacaatc
     4261 atgatttgaa gaatacactt attttatcgg cgctgcctgc ggcggatgaa acgtgtttgg
     4321 cgcgcaagac gctgcttcgt gccgtgggca ggatgtggct ggccggcgcc cggattgact
     4381 gggaaaattt acattcgcag gaaccgagaa agcgcattcc gctccctctt tatccctacg
     4441 agaaaaagag gtattggatt tccagagtct ctgcaggacg gattccgcaa accggtacga
     4501 tccgctttac cgaataaagg gtcatccggc catcgtggcg ggcagaaaca gtgccagttg
     4561 agggaagagg aataacagaa ccacacagat caggatggcg ataaaaaagg gagcgatgcc
     4621 ccggaagatt gttgccatgg gaacattctt ggcgattccg gaaatgataa agacattgat
     4681 tcccacgggc ggcgtgatca ggcccatctc aatgagcaga atcatgagca ccccgaacca
     4741 gatcgggtcg aaccccattg ccgtgaccat cggaaacacg atgggcaggg tgagaaacat
     4801 gatggcaaat ccttccagga tgcaccccag aacaacatac accagacaaa tgatacccag
     4861 cgtcgcatac cggttcatgc ctgtgtccgt gacccaggcg gtcactgtcg ccggcaaatc
     4921 gctcagcgtc ataaagtagc cgaagatatt tgctccgatc agaatggcaa aaatcatggc
     4981 ggtggtcagg gctgtttccg tgagcgaacc ggccaaaagc ttccaggtaa gtcttttttt
     5041 tgcagaaata atgaccagaa caatcaacgc tcccaccgcc gccgcttctg tcggtgtgaa
     5101 ccagcctccg taaagtccgc ccaccaccag cgcgaaggtc aaaagaacgc ttccggtgtc
     5161 ccggagggat tcccattttt cagcgaaaga aaatcgggaa tccggatgac tgatgccgtg
     5221 acgtttcctg ctccacagaa agatcgtcaa aagaaaaagc cctgtgagga tgatgccggg
     5281 aatgatgccg gcgataaaga gcgttccgac gggttgtccc gtaagaatgg cgtagaccac
     5341 cataaccgtg ctgggcggaa tcaaaatgcc cagtgttccg cccgcggcca cacatccggt
     5401 cgccagcgcg tcatcgtatc ggaagcgttt catttccggc aatgcaaccg cgccgatggt
     5461 ggctgccgtc gctaaagagg agccggagat cgcggaaaat ccggcgcacg cgccgaccgt
     5521 tgccatcgcc agcccgccgg gcagaaagcc ggtccagcgg tgaacggcac gatataattc
     5581 aacgcccatg ccggattgac cggccagttg tcccatcagg aaaaagagcg gaatgacggt
     5641 cagggcgtag gaggagacgg tctgatagac gaccatgccc agcatggaaa aggccgcgtc
     5701 cggagaggac aggatggaaa atcccgccag accgacaatg cccattgcgc agccgatcgg
     5761 aacgcgaatc atgatgagga cggccatgac gatgaggccg aatacgccga cggttaccgg
     5821 ggtcatagca ctttttcatc ctttatcgat gccagggttt ttttaaaaaa gacgaaactg
     5881 agaacggcag cgccgaaggc aatgacatag gcgaagggat atttgggaat ctggagatcc
     5941 atggatacgt caccggatag tcttagcgaa gagccgtaaa caatcgccgc cgccgtgatg
     6001 agcgcaaaaa acagggcatt ggcaagagac gtgatgacgg ctaaatattt cctggccccg
     6061 gaggtcaggc ggtctgtcaa aagggaaacg ctgacatggc gatccatgat ttccgcttgg
     6121 gcaagcgcga aaaagacaag gatgatcatc atcaattcgc tgatttccgt cattccggtt
     6181 atgctgatgc ccgtcagctt tctcaaaaga acattcgccg cggttagaat catcaggata
     6241 aaaatgatcc ccgcggcgac gccgtttaag ccggttgccg ttttgtgtat gcaggaaaag
     6301 accctcttca caaccgcacg cgccctattt tattttagcc gaagcgcggt gtatggcatc
     6361 catcatctca cgtgcaggaa ttcccttctc gctgttttgc cggatccagt cgctttccat
     6421 gcctgccaat tggttccgga ttttctttct ttcgtccggg gccatgtcgt gaattttcac
     6481 gttgcctgac gtccgcagct gattgacagt ttccctggag taatcgtcat acacaccggt
     6541 ggcgtgaagc ccccaggatt ttccggccgc gttgtcaatg gcctgtttgg cggcgggaga
     6601 cagcgcctca tatttcttct tattcatgac cacggccatg atggaaatat aaaaatccgt
     6661 ttgggtaata tgtttgattt gatcgtagag tttgaagtcc gcgacaaccg tattgggggt
     6721 cagcgcgcca tcgataacgt gttttgcaag ggccatgtgc atttccgtga tcggcattcc
     6781 caccggattg gcgcccagcc gcttcagggc gtcgctcatg gacgcggaag gcgaccgcaa
     6841 tttcattccc ttgaaatcct ccatcgtcag gatcggctta tcgacggaat ataactgtcc
     6901 catgtcggtg caataaatcc agagcagttt atagtccttg aaatcttcgg caaaccagcg
     6961 ttcataaagg tcgtaagcca cctggatggc ctgctgcgga ttatcgaaga ggaacggcag
     7021 atcatacgcc gaaaatcggg gaaaccggcc ggaggtcatg gacggaaaga tcagagcgat
     7081 atccgcgata cccgttttga cggcgtccgg aagttgcacc gctcccccga gagtgcccgc
     7141 tggatagatt ttaatcttca ccttgccgcc ggacagtttt tccacatttt gggcgaaggg
     7201 caggaaggtc ttctggtgtt gaatatgcgt ggacggtatg aaatgcgcca gcttcaattc
     7261 caccgcatcc tgagccgcga cagggccggc cgtcaataat accccgcaga tcatcaataa
     7321 tatcaaataa tgctttttca ttattttatt tctcccctga agatttttta tgattataga
     7381 aaaaaaacaa ttcgtgtcaa tgccttttgc acagcaggcc ctgttgaatg ttttgattga
     7441 cttctgcagt attttttaat ttattgaaag aaaaaaatga aggaatggat cgatgatgga
     7501 catcgaagcg ctgataccgc accgcgagcc gattaaagtc atcacggaaa ttcttgagct
     7561 cgatgacgat tccggttcgg ccgcggccgt ggtcaacgaa cgctggccgc tgtgcgacgg
     7621 caaggcggtg tgtgcgcttg ttttaattga agccattgcc cagacgtccg ctctgatcga
     7681 agggcataaa cggatgaaaa agagcatggg cggcacaaaa gggtggctgg tcggcatcaa
     7741 aaccgccgaa ttcaggacgg cgacggtgcc cctgggtacg cggataacgg ttaccgttaa
     7801 aggcctttct tcatttgatg aatacggtgt catcgagggt gtggtaaagg cgggcgacga
     7861 cattttgctg tcggccgtgc ttcaggcaat ccgattcaac gggcacgggg ttgaacaaga
     7921 tattcgagga tgatgtgccg atcatggaag aaaaaaagac agcccttgta acgggcgcaa
     7981 gccgggggat aggacgggcc accgccgtgg aactggccaa aaacggttac tttgtggtga
     8041 ttaattacaa atccaatgag gcggcagccg ccgaaacgct ggttcaggtt gagcaggcag
     8101 gatccggcgg aacattgtgc cgatttgatg tggcaaacgc agaagaggtc cggacagcgc
     8161 ttgatcaaat cataaagacg cacaaaagag tcgatgtcct gattaataat gccggcatca
     8221 cggccgacgg cctttttgtg atgatggatg aggaggactg gctcaatgtc gtcaacacga
     8281 cgttgaacgg attctatcac gtgaccaggc ccgtcttgag ggaaatgatt cgaaagaaac
     8341 gcggggccat tgtttccgtt tcttctgtct cctcgatcct gccgaatcgc ggtcaggcca
     8401 attatgctgc ggccaaggcc ggcatcaacg ccgccacgcg ttcgctctcc aaggaagtgg
     8461 cgcgtttcgg cattcgggtg aatgttgtcg cgccgggtcc cgttgaaacg gacatgatcc
     8521 gcgacatgcc gatggatgac atcaaaaaaa tcattcccat ggcgcgtgtg ggccggcccg
     8581 aagaagtcgc caaagtgatt tgttttttat gttccgacga tgcgtcttac gtgacagggc
     8641 aggtcatcgg cgtcaatggc gggatgtttt aatttttcac aaaaatccga tgataaagaa
     8701 aaatgcttct tgcttctgaa aaaatgcccg tcatgaccct cgcggaaaa
//
LOCUS       DAOOLB010000080         8642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_9558_length_8642_cov_31.953767, whole genome shotgun sequence.
ACCESSION   DAOOLB010000080 DAOOLB010000000
VERSION     DAOOLB010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9558_length_8642_cov_31.953767"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="PLF77_08965"
     CDS             complement(<1..274)
                     /locus_tag="PLF77_08965"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC09022.1"
                     /translation="MKEKEMEIRYDMTGVDWKDVAQTLKSVGMSYHEPDEHRRAFAAS
                     HTTVFAYRGDRMIGFGRALADGVYQAALYDIAVVPEFQGQGVGTLIV"
     gene            583..2040
                     /locus_tag="PLF77_08970"
     CDS             583..2040
                     /locus_tag="PLF77_08970"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC09023.1"
                     /translation="MRKIEDQNQEFKQVWNDNCLKTICAFANTTGGKLYIGIDDEGNA
                     TGVKDAARYLDDIPNKTKDILGITPAVKIKKKAGKEIIEISVEPSSAPISYHGRFFTR
                     SGSSSIEIKGHELVEMLMRKSGRSWDGFIEEDATLKDIDTAAVNKFRKLATKKIPVIA
                     KEKNVTAILQKLNLLKRGKLRRAALLLFGKNPKKFYITAYIKIGKFISSTDLISTDDI
                     EGNIFDQVDRAIEMLRVKYLLSNITYEGIYRKDNMEYPEEALREAIINAVIHRNYMGA
                     HTQLRIDPDSLNLWNEGTLPPDIKIDDLKKWHLSRPRNEFLADAFFKAGMIEAWGRGT
                     IKIVDECKKAGLPEPEFRQEFGGLSVRFRKADKAHERAQVEDQVGTKSKQHRVQIGRQ
                     LESQPESRPESRPESLDIRIIRRLVPTPLSKAELSALLGQKEISGQLNKIIRELLQKK
                     VIEYTIPDKPNSRMQKYLLTDKGRKILVTIQGDKK"
     gene            2180..3805
                     /locus_tag="PLF77_08975"
     CDS             2180..3805
                     /locus_tag="PLF77_08975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPC09024.1"
                     /translation="MNKKQKLELTWIGKENRPRLEPRILLEEPKKSYHALHRVSGKDI
                     FDNQLIFGDNLLALKALESEYAGKVKCVFIDPPYNTGSAFTHYDDGVEHSIWLSLMRD
                     RLEIIRRLLSENGSLWITIDDNEAHYLKVLCDEVFGRGNFMTNVVWQKRTSPDNRLRL
                     GDAHDSILVFRKNKFSGNESFNQLPVTDCRTKDFKNPDNDPRGPWASTDFTGMTGHAT
                     PNQFYTITSPNGTMHPPPDGRCWALSEETFKKYLAENRVWFGKDGKARPRLKRFLNEL
                     EGQNSWSWWPNTEVGHNQEAKKEVVALFGANSTFDTPKPERLLQRVLTIATNPGDLVL
                     DSFAGSGTTGAVAHKMGRRWIMVELGDHCHTHIIPRLKKVIDGEDSGGITKAVKWKGG
                     GGFRYYRLAPSLLEKDKWGNWVINKTYNAAMLAEALCKLEGFTYAPSDTVYWQHGYST
                     ERDFLYVTTANLTHQQLLQLSDEVGPDRALLVLCTAFRGKGEYPNLTVKKIPRQVLSR
                     CEWGHDDYSLQVENLPQAPPKAGQQSLFTEEES"
     gene            3802..5007
                     /locus_tag="PLF77_08980"
     CDS             3802..5007
                     /locus_tag="PLF77_08980"
                     /inference="COORDINATES: protein motif:HMM:NF036995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HPC09025.1"
                     /translation="MKAKPSERRLKNKPAIYDRILRILESARSSVTRSVNTSQVVANW
                     LIGREIVEEEQQGKVRAGYGELLITALAEQFKGDGIAGYSVQNLRYMRQFYLTYSELI
                     AIPTIRHALRGKSSKTDVPHAEKNDQRKADGETYFAASQIHHATRGESWRPGLLHPNL
                     SWTHYRTLLRVDKPEARAFYEVEAIRNNWAARELERQINSLLYERLALSKDKKGLMRL
                     AAKGQEIQKSADIFKDPVVMEFLGLPASPKLTESALEQALIDNLQVFLLELGRGFAFV
                     ARQQRITLDSDHFYIDLVFYHTILKCYVLIDLKVGKLTHNDLGQIQLYVNYFDKERRA
                     ADDNPSLGLILCTDKNDAVVRYTLGENRQDKIFASRYKLYLPTEEELKAELRREVKLI
                     GPAPGGGKP"
     gene            5004..>6628
                     /locus_tag="PLF77_08985"
     CDS             5004..>6628
                     /locus_tag="PLF77_08985"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPC09026.1"
                     /translation="MNKHVNSIAGRLSLRPPQRRSLEILDRIMEITPPGKSSDSATRL
                     SAIQSEFPSIADFEREFPSLCFALATGVGKTRLMGAFISYLHLAHGINNFFVMAPNLT
                     IYNKLIADFTPNTPKYVFKGIAEFATDAPSIVTGDNYEGMAVTLFDEFLRCKINIFNI
                     SKINTEVRGGKSPRIKRLSEYIGQSYFDYLAGLPDLVLIMDESHRYRASAGVRAINEL
                     KPILGLELTATPFMETPRGPAPFKNVIYDYPLGRAMEDGFVKEPAVVTRRDFNPAGMS
                     PEEIEKMKLMDGVRLHESVKVELETYARESGNPIVKPFLLVIARDTTHAGALMQLIGS
                     EAFFEGAYREKVIQVDSSKAGREEEEMIERLLQVESTSEPTEIVIHVNMLKEGWDVTN
                     LYTIVPLRAANARILIEQSIGRGLRLPYGKRTGVTAVDRLNIVAHDKFQEIIDEANCP
                     DSVIHMQAVVLDEAALAKKTVTVVSQSNLATKLGIRPQQETGSATIAGQNQTPVFAKP
                     EEQEAAKIAWEVIRKMENQPTILPDITHLKKPE"
     assembly_gap    6629..6728
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6729..7336
                     /locus_tag="PLF77_08990"
     CDS             <6729..7336
                     /locus_tag="PLF77_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09027.1"
                     /translation="KVLACYQRDIARFIHAQMLDHYWEEAAGYEVVVSKGYTELKESA
                     YTYGISEPPLDYHVSPTDKSNMAKYLFNGFERCLYPVQKFASDAERKLAVILDRDSIK
                     WFKPAKGQFQIFYRQGADHLEYQPDFVAETGAMIFMLEPKARNEMNDEIVLAKKEAAL
                     NWCANASNHAQSNGGKSWRYLLIPHDEIAENMTLDGLSKRY"
     gene            7404..8384
                     /locus_tag="PLF77_08995"
     CDS             7404..8384
                     /locus_tag="PLF77_08995"
                     /inference="COORDINATES: protein motif:HMM:NF025454.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4268 domain-containing protein"
                     /protein_id="HPC09028.1"
                     /translation="MGTKTLGRLQKVELREAWTTESGDFTPWLAKEENLSLLGETIGI
                     ELELESQEKDVGPFRADILCKDTATDNWVLIENQLERTDHTHLGQLLTYAAGLNAVTI
                     VWIAERFTAEHRAALDWLNEKTDEKINFFGLEIELWQIGDSPIAPKFNIISQPNDWFR
                     AVQKAAAGDGEISAHKQFQLKFWTAFKQYMEEKGSFVRCQKPLPQHWTNHAIGRSGVH
                     LDSIISSWNSETGMKGPEIRAELYLDGPNAKHEFAALEKQKESIEKALGFALTWHNPE
                     NKAACRLYTRQSYNFMDETSWTEQFEWLRARLETMHKVFAPIMKSIKIMT"
BASE COUNT         2364 a         2046 c         2167 g         1965 t
ORIGIN      
        1 gcacgatcag cgtgccgacg ccctgtccct gaaactcggg aacgacggcg atatcgtaaa
       61 gggccgcctg gtaaacaccg tccgcgagag cccggccgaa tccgatcatc cggtcgcccc
      121 gataagcaaa gaccgtcgtg tggcttgcgg caaatgccct gcggtgttcg tcaggctcat
      181 gataactcat gccgacgctt ttcaacgtct gagccacatc cttccaatcc acaccggtca
      241 tgtcgtatcg gatttccatt tccttttctt tcacaaaatg ttctcgtgtt tgagagcgta
      301 ccacttccct gaagaatatg caaagatcgc cctgtgcatt aaagaaggag tcaaggtctg
      361 ccttagacct ttgatgccca gttctatttg ctataattct ttctgtaatt tgcaatatca
      421 aaagccgcac cgatttcaac gagctttttc ccgttgtctc gccccttgaa ctttttcacc
      481 atccaccaat tacattttcc ttgcattgat ataataaaat tgtaagatgt cgaaaatcat
      541 gatgttctca taataccaat tggaaacggg cgaagaatca tcatgcgcaa gattgaggat
      601 caaaatcagg aattcaaaca ggtctggaat gataactgtc tgaagaccat ttgcgcgttt
      661 gccaatacca ccggggggaa actctacatc ggcattgacg acgaaggaaa cgccaccggc
      721 gtgaaagacg ccgcccgcta tctggatgac atacccaaca agaccaaaga cattttaggc
      781 attacgcccg ccgtcaagat caaaaagaaa gcaggcaaag agattattga aatatcggta
      841 gagccgtctt ccgcgccgat ttcgtatcat ggccggttct ttacccgcag cggcagctcg
      901 tcgattgaaa tcaagggcca tgaactggtg gaaatgctga tgcgcaaatc cggccgttcc
      961 tgggacggat ttattgaaga agatgcaacg ctcaaagaca tcgacaccgc cgccgtcaat
     1021 aaattccgga agctggctac taaaaaaatc cccgtcattg caaaagaaaa aaatgttacg
     1081 gccatcttac agaaattaaa cctcctgaag cgcggcaaac tacgccgcgc cgctcttttg
     1141 cttttcggca aaaatccgaa gaagttctac atcaccgcct acatcaagat cggcaagttt
     1201 atcagctcga ccgacctgat cagcacggac gatattgaag ggaatatttt tgatcaggtg
     1261 gaccgagcca tagaaatgtt gcgcgtcaaa tatctgcttt ccaacatcac ctatgaaggc
     1321 atttaccgta aagacaacat ggagtatcca gaagaagccc tgcgggaagc catcatcaat
     1381 gcggtgattc accgtaatta catgggagcg cacacacagc ttcggattga ccccgacagc
     1441 ctgaacctct ggaatgaagg aacgttgccg cccgatatca aaatcgacga tttgaaaaaa
     1501 tggcatctgt cccgcccgcg caatgagttt ctggccgacg cttttttcaa agcgggcatg
     1561 attgaggcct ggggccgcgg cacgattaag atcgttgatg aatgcaagaa agccggactg
     1621 ccggaaccgg aattccgcca agagttcggc ggcctatccg tacgcttccg gaaagcggat
     1681 aaggcccatg aaagggccca ggtcgaggac caagtcggga ccaagtcaaa acagcaccgg
     1741 gtgcaaattg gaaggcagct agagtcgcag ccagagtcac ggccagagtc acggccagag
     1801 tctctagata tcaggattat tcgacgcctt gttcctacgc ctttgagcaa agcagaattg
     1861 tctgcattgc ttgggcagaa agaaatatcc ggccaattaa acaagattat tcgtgaactg
     1921 ttacaaaaga aagtaattga atatacgatt cccgataagc ccaacagccg catgcaaaaa
     1981 tacctgctta ccgataaagg ccggaagatt ttggtcacga tacaaggaga taaaaagtaa
     2041 aaacgtgatc gcacgcgttg ggcccgctaa aggtagttac tggaagatag aaatctaaat
     2101 gtacctgtgc cattaattta tataccaaag agttattcga ttatgtgatt tcaaaggtgc
     2161 ctcagataag aaacttgaca tgaataaaaa acaaaaactt gaattaacct ggattgggaa
     2221 ggaaaaccgg ccgcggctgg agccgcggat tttactggaa gaaccgaaga aatcgtatca
     2281 tgcgcttcac cgggtgagcg gtaaagatat tttcgacaat cagttgatct tcggcgacaa
     2341 cctgctggca ttaaaggcac tggagtcgga atatgccgga aaggtgaagt gcgtattcat
     2401 cgatccgcct tacaatacag gcagcgcttt tactcattat gatgatgggg tggagcattc
     2461 catctggttg tcgctcatgc gtgatcggct ggagattata cggcgattat tgtcagagaa
     2521 tggttctctc tggattacga ttgacgataa cgaggcgcat tacctgaagg tcttgtgtga
     2581 tgaagtcttc gggcgcggaa acttcatgac caacgtagtt tggcaaaaaa gaacatcgcc
     2641 ggataacagg cttagattgg gtgatgcgca tgatagcatt ttggtattcc gaaaaaacaa
     2701 attctctgga aatgaatcat ttaaccaatt gcctgttact gattgtcgaa caaaggattt
     2761 taaaaatcct gataatgacc caagaggacc ttgggcttcg actgatttca ctggcatgac
     2821 aggtcatgct acgccaaatc aattttatac aattacttct ccaaacggca ctatgcatcc
     2881 tccgcccgat ggcagatgtt gggcattatc ggaagaaact tttaaaaaat atttagcaga
     2941 gaatcgagtt tggttcggca aagatggcaa ggcgagaccg agattaaaac gatttctaaa
     3001 tgagcttgaa ggacaaaatt catggtcatg gtggccgaac actgaagttg gacataatca
     3061 agaagccaag aaagaagttg ttgctctttt tggcgctaac tccacatttg atacaccaaa
     3121 acctgaacgc ctattgcaga gagttctaac tatcgccacc aaccccggcg accttgtcct
     3181 tgactctttc gccggttccg ggaccaccgg cgcagtggcg cacaagatgg gacggcgttg
     3241 gatcatggtg gagctgggcg accattgtca cacacacatt atcccgcgtt taaaaaaggt
     3301 gatcgatggc gaagattcag gcggcattac caaagcggtt aaatggaaag gcggtggcgg
     3361 attccgctat taccgccttg cgccttccct tctggaaaaa gacaaatggg gaaactgggt
     3421 catcaacaaa acttataacg ccgccatgct ggcggaagcg ctctgcaagc tggaaggttt
     3481 tacgtatgca cccagcgata ccgtttattg gcagcacggc tactccacgg agcgggattt
     3541 tctttatgtg acgaccgcca atctcacgca ccagcagctt ttgcagctct ccgacgaggt
     3601 cggaccggac cgcgcgctgc tggtgctctg cacggctttc cgcggcaagg gcgaatatcc
     3661 taatctgacc gtcaaaaaga ttccccggca ggtgctttcc cgctgtgagt ggggtcatga
     3721 cgactacagc ctgcaagtgg aaaatctgcc ccaagcaccg cccaaagccg ggcagcagag
     3781 tcttttcacc gaggaggaat catgaaggca aagccatccg aaagaaggct gaaaaacaag
     3841 ccggcgattt acgatcgcat tttgcgcatt ctggaatccg cccgaagttc cgttacgcga
     3901 tccgtcaaca catcacaggt tgtggccaac tggcttattg gcagggagat tgtggaagaa
     3961 gagcaacaag gaaaggtcag ggccggctat ggagaattat tgataacagc attggctgag
     4021 caattcaagg gcgatgggat cgccggttat tccgtgcaga atctgcgata tatgcgtcag
     4081 ttttatttaa cgtattccga actgattgcc attccaacaa ttcgccacgc actgcgtggc
     4141 aaatcttcta aaacggatgt tccccatgcc gaaaaaaacg accagcggaa agcggatggc
     4201 gaaacttatt tcgccgcctc tcagattcac cacgcaacgc gtggcgaatc ctggaggccc
     4261 ggccttctgc atcccaacct ttcctggacg cattaccgca cactgctgcg cgtggataag
     4321 ccggaagccc gcgccttcta cgaggttgaa gcgatccgga acaactgggc ggcccgtgaa
     4381 ctggaacgcc agatcaacag tttgctttat gaacgccttg cgctcagcaa ggacaaaaaa
     4441 ggactgatgc gactggccgc aaaaggccag gaaattcaga aatccgccga tattttcaaa
     4501 gacccggtgg tcatggagtt tctgggtctt ccggcatcgc cgaaattgac ggagtcggcg
     4561 ctggagcagg cgctgattga caatttacag gtattcctgc tggagctggg cagggggttc
     4621 gcctttgtcg cccgccagca aagaattacg cttgacagcg accactttta catcgatctg
     4681 gttttctacc acaccatcct caaatgctac gtcttaatcg atttgaaagt cggcaagttg
     4741 acccacaacg atctgggcca gattcagctc tatgtaaatt atttcgataa ggaacgccgg
     4801 gctgccgacg acaatccttc cctggggctg attctttgta cggacaaaaa cgacgccgtt
     4861 gtcagatata cgctgggtga aaaccggcag gataaaatct ttgccagccg ctacaaactt
     4921 tatctgccga cggaagagga gctaaaggcg gaacttcgcc gcgaagtgaa attaatcggt
     4981 cctgcgccgg gtggagggaa accatgaata aacacgtgaa ttccatagcg ggacgcttga
     5041 gcctgcggcc cccgcaacgc cgctccctgg agattctgga ccgcatcatg gagattaccc
     5101 caccggggaa aagctccgat tcggcaacca ggctttcggc catccaaagc gaatttcctt
     5161 cgattgccga ctttgagcgg gagtttccct ctttgtgttt tgccctggcc accggcgtgg
     5221 ggaaaacaag gctcatgggc gccttcatca gttatttgca cctggcccac ggcatcaata
     5281 atttttttgt gatggcgccg aatctgacga tctataacaa actgatcgcc gattttacgc
     5341 ccaacacacc caaatatgtt ttcaaaggca tagcagaatt tgccacggat gctccgtcca
     5401 ttgtcaccgg cgataactac gaaggcatgg cggttacgct gtttgacgaa ttcctgcgct
     5461 gtaagatcaa catcttcaat atttctaaaa tcaatacgga ggtgcgcggc ggaaagtcgc
     5521 cgcggatcaa aaggctttcc gaatatatcg gccaaagtta ctttgattac cttgcgggac
     5581 tgccggatct ggtgctgatt atggacgaat cccaccgcta ccgcgcaagc gccggtgtgc
     5641 gggccatcaa tgaactcaaa cctattctgg ggctggagct gactgctacg ccctttatgg
     5701 agacaccacg cggccctgcg ccatttaaga atgtgattta cgattatccc ttgggcaggg
     5761 cgatggaaga tggttttgta aaagaaccgg ctgttgtgac gcgcagagac ttcaatccgg
     5821 cggggatgtc gccggaagag atcgaaaaaa tgaaactgat ggacggagtg cgtttgcatg
     5881 agagtgtgaa agtggaactg gaaacctatg cccgggaaag cggcaatcca atcgtcaaac
     5941 cctttctgct tgtaatcgcc cgcgacacaa cccatgcggg cgctttgatg caacttatag
     6001 ggtcggaggc gttttttgaa ggcgcgtatc gtgaaaaagt tatccaggtg gactccagta
     6061 aggccggccg ggaagaagaa gagatgattg agcgcctcct tcaggtcgaa tctacatccg
     6121 agccgacgga aatcgtcatc catgtgaata tgctcaaaga aggatgggac gtgaccaacc
     6181 tctatacgat tgtacccctg cgcgcggcca atgcgcgtat tctgatcgag caatccatcg
     6241 gtcgggggct tcgcttgccg tacggcaagc gaaccggtgt tacggctgtt gaccggctca
     6301 atatcgttgc ccatgacaag tttcaggaaa ttattgacga ggccaattgt cctgattcgg
     6361 tcatccatat gcaggcggtt gtcctggatg aggcggcgct ggcaaaaaag acggtcaccg
     6421 tggtttcgca gtccaacctg gcgaccaaac tggggatcag gccgcagcaa gaaaccggca
     6481 gtgcaacgat tgccggacaa aatcagacac cggtttttgc caagccggaa gaacaagagg
     6541 ctgcaaaaat cgcctgggaa gtaattcgca agatggaaaa ccagcccaca atactaccgg
     6601 atattaccca tctgaagaaa ccggaaatnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6721 nnnnnnnnga aaggtgctcg cctgttacca gcgggatatt gctcgtttta tccatgcgca
     6781 aatgcttgac cattattggg aagaagccgc aggatatgaa gtggtggtca gcaaaggtta
     6841 tacggaattg aaggaaagcg cttatacgta tggaatttca gagccgcctc ttgattacca
     6901 tgtgtcgcct accgacaaga gtaatatggc aaaatatctc tttaacggtt ttgagcgctg
     6961 cttgtatcct gtgcagaaat tcgcttcgga tgccgaacgc aagcttgccg tgattcttga
     7021 ccgggattcc atcaagtggt tcaaaccggc caaagggcag tttcagatat tttaccgtca
     7081 aggcgccgat catctggaat atcagcctga ttttgtagct gaaactgggg ccatgatttt
     7141 catgctggaa ccgaaagcca gaaacgagat gaacgatgaa atcgttttgg cgaaaaaaga
     7201 agcagcttta aattggtgcg ccaatgcctc gaaccatgcg caaagcaatg gcggcaaatc
     7261 ctggcgctac ctgctcatcc cgcatgacga gatcgccgag aatatgacgc tggatggatt
     7321 gtcgaagaga tattgaacgg ttatttatgc cgacaaaccg gcagctaaaa gctcatcggt
     7381 gattaagaat aaagggaata aaaatgggta caaaaacact tggccgtttg cagaaggttg
     7441 aattacgcga agcatggaca acagaatcag gcgattttac tccttggctt gccaaagagg
     7501 aaaatttgag cctgctgggt gaaactattg gtattgaact cgaactggaa tcgcaggaaa
     7561 aagacgtcgg gccatttcga gctgatattt tgtgtaaaga cacggcaacc gacaattggg
     7621 tattgattga aaatcaattg gaacgcaccg atcacacgca cttggggcaa ttattgacct
     7681 atgccgcggg cctgaatgca gtaacgattg tctggattgc agaacgtttc accgccgagc
     7741 atcgcgccgc tttggactgg ctcaatgaga agaccgatga gaaaataaat ttttttggtc
     7801 tggaaataga attgtggcag attggtgatt caccgattgc ccccaaattt aatattatca
     7861 gtcaacccaa tgactggttc cgtgcggtac agaaagcggc ggccggcgat ggcgaaatat
     7921 ctgctcataa acaattccag ttgaagttct ggacggcatt taaacagtat atggaagaga
     7981 aaggcagctt tgtccgatgc caaaaaccct tgccccaaca ttggacaaat catgcgatcg
     8041 ggcggtctgg tgtccatctg gattcgatca tatcttcatg gaattcggag acaggtatga
     8101 aaggaccgga aatacgtgca gagctttatc ttgatgggcc taacgctaaa catgagtttg
     8161 ctgcattaga aaaacaaaaa gaaagtatcg aaaaagcatt gggctttgcg cttacatggc
     8221 ataatcctga aaacaaggcg gcatgccgac tctatacaag acaaagttat aattttatgg
     8281 acgaaacctc atggacggaa caatttgagt ggcttcgggc acggttggaa acaatgcata
     8341 aggtttttgc tccgataatg aaaagtatta aaataatgac ttaacagggt caaatggagg
     8401 tcaggtttgc ctttgacctt tgatggacgc cccccattat ttaaagacac ggccggtttg
     8461 agcagggcaa ggtttggacg ggcatagcga cgggaatctt tcctataaac gaaaggctga
     8521 aacgcagcgc gcttcagcct ttcgttcact tttctggatt cccgccttcg cgggaatgac
     8581 aaatttttcc tagcggctgc ggaacggtcg gggaccgttc cctacatcta cagcctatag
     8641 cc
//
LOCUS       DAOOLB010000081         8612 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_9605_length_8612_cov_36.435316, whole genome shotgun sequence.
ACCESSION   DAOOLB010000081 DAOOLB010000000
VERSION     DAOOLB010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8612)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8612
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9605_length_8612_cov_36.435316"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..998
                     /locus_tag="PLF77_09000"
     CDS             <1..998
                     /locus_tag="PLF77_09000"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HPC09029.1"
                     /translation="ANITGMQNDIGRLAWLAGGFPESVPTNTLTMQCPSGMAATQHAA
                     RAIMCGEGDTYIASGVEDMQKVFMAMHMDFPPRLANRYNILELPMGNTAEKVAEVYKV
                     SRADMETMAYWSNKKADEATKAGKFKNEIIPMEGLKDDGTPFMVETDQWIRGDVSMEQ
                     MATMKTPFKPDGVVTAATSSPLTIGAAAILLMERSKADKLGLSYSIKYEAGALGGCDP
                     TVMGIGPIPAVQRLLARTGKKVSDIGVWELNEAFASQSLACLRELGIAQNAPFDNVNI
                     WGGALALGHPLGESGCRILVTLHSIMKTDRKDAKYGVASLCGAFGNAGATMLSRV"
     gene            complement(1096..2043)
                     /locus_tag="PLF77_09005"
     CDS             complement(1096..2043)
                     /locus_tag="PLF77_09005"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPC09030.1"
                     /translation="MKTLKALIPILLLCIMAVTSGCTALGWNTVPVEKLKLKYADAES
                     EIIEIDEMKIHYKDEGQGPVLILLHGVCASLHTWDGWTQRLKDRYRIIRLDIPGFGLT
                     GPAPDKSLYRKDEAVRLFEKVVDEMKLEKFYLAGNSLGGYISWNYTLKHPDRVEKLIL
                     VDSVGFPQPLPGLLAFASNPLIRPFARFTMPRFLFDNAVEQVFGDKSKVTQELKDRYF
                     DLAMREGNKGSYIDVFTEMRKLCRDENLSQGIKDIRVPTLVMWGTRDEWIPFKYFENW
                     KKELPGAKFIQYEGAGHTPMEEIPDETARDADLFLSGKM"
     gene            2265..2705
                     /locus_tag="PLF77_09010"
     CDS             2265..2705
                     /locus_tag="PLF77_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09031.1"
                     /translation="MKTYAIKYLELAEKHAEKIAARWLKDVKNNSRTPTYKSLNEAEI
                     TNQCVRFYQTFSKMFLDEKITDDSLRYFRSYAEESHTMGIPAKETLYALILMRRHIWL
                     YAEFQAIFTSGIDQRQALDTLGRTILLFDYACYEVTKEYQSLTR"
     gene            2776..3216
                     /locus_tag="PLF77_09015"
     CDS             2776..3216
                     /locus_tag="PLF77_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09032.1"
                     /translation="MVDSIKLLDITEQNAEEIAEHWAKEVHKNKRTTHYRNIKKEKLV
                     VYAVDFYRNLRKLLVPEDRLEYTREYFQKYAKKCHEIGLPLQEAIYGLVLMRRQMWLY
                     ADFQAIFIDALEHNQAIDSIMRIMLMMDYAVYEITQYYLEIAKH"
     gene            3613..5454
                     /locus_tag="PLF77_09020"
     CDS             3613..5454
                     /locus_tag="PLF77_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HPC09033.1"
                     /translation="MASLWVDLRDIKFQLFEVLKIEETLLGKGRYKDFDADTCIMALD
                     AAAKFAENDIAPTYPDEVHRKPVEATFDKGKVTTPEAYKRLWKLYCENGYMAMADQPE
                     VGGQGFPAVIAAAAANMFLACNQAFVMYPGLSHGCARLMEDFFQHPLRDIVLEKMYTG
                     EWAGTMCLTEPGAGSDVGALKTTAKKNADGTYSIVGTKSFISAGDHDLTPNIIHPVLA
                     RIEGDPKGTKGISIFMVPKIRFNEKGELGEPNDVQCGNIESKMGIHGNATCTLNFGSD
                     NKCIGYLMGEERQGMPIMFHMMNEERQGVGMMGASLAMAAYLHALDYSKQRIQGQDVI
                     MMAMKVEDAPAVPIIKHPDVRRMLLKQKAISEGLRLLAYFCYFCMDNEFSAMIDGNEE
                     QKEYWHGLIEVLTPIVKSYCTEKGQDSIELAVQCYGGYGFCREYPVEQMMRDNKINLI
                     YEGTNGIQALDLLGRKLGMKRGAYFMNLLSVTKEAIEEGKKLDAFKKEAETVEKALNA
                     CAETALAFSKMIKTTPFVPLIGACDFLNCLGDALVGWLHLRMALVAIPKFFAADTGEM
                     DKAFYRGKIEGARFFINRITGLVIPHLENLKQDEQSAMNIPEESFAV"
     assembly_gap    5881..5980
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5981..6268
                     /locus_tag="PLF77_09025"
     CDS             <5981..6268
                     /locus_tag="PLF77_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09034.1"
                     /translation="QQLHFMKEEILVKLNNLSGKPLIKEIRFVVGHSPVNEKEANPVL
                     KEKKAVLKKRDRDMIAECTEALIDQELAAVLKRIMRLEISRRRQWESRKGR"
     gene            complement(6228..6992)
                     /locus_tag="PLF77_09030"
     CDS             complement(6228..6992)
                     /locus_tag="PLF77_09030"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HPC09035.1"
                     /translation="MIRTMEEETLDEILHGALRIFQKKKGYRFSIDSILLAHFVSLKK
                     RTRLMDIGCGSGIILLILTKRFPTTQCVGLEIQERLAVMARKNVQLNQLENRIDIVWG
                     DARNVRTIFQERTFDAVVFNPPYRKLHSGRINPLEERAIARHEIQGSLKDFLNAAKYL
                     LKPAGTVFTIYPAKRLVELVYLFRLHGIEPKKMKLIFSDVSSGAEFVLVEGRKGSKEE
                     LKVEPPMFIYDRPGKYSEKMSGVFSDLSGFPIDDAG"
     gene            complement(7002..8243)
                     /locus_tag="PLF77_09035"
     CDS             complement(7002..8243)
                     /locus_tag="PLF77_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPC09036.1"
                     /translation="MAIVLPFKAVRPDKKFVAQVAALPYDVMTREEGKKAVSGNPLSF
                     MHVEKSEIDVPDKTKSDDPLIYQTAKRNFTDMRVKGILIQDESPHFYVYRQKMGTRAQ
                     TGIVGVMNASEYDAGKIKKHELTRQDKEEDRIRHVDTVNAQTGPVFISYRERPGINTI
                     VDHVTFGMPEYDFTSDDGVTHTVWIISDSRHIEEIKKEFLAVDALYIADGHHRAAAAA
                     AIARTRRAKESARGFAGEYESVLAVFFPDTQLRVMDYNRAVKDLNGLTPDQFIDKIGS
                     AFIVSKDFSDRSPNQLHDFGMYLGGQWHKLTVKEGVYDKNDPVASLDAAILQEHLLYP
                     VLGIKDPRVDDRIKFIGGIRGMDELEKLVNQDGFAVAFSLYPTTMEQIINVADAGAIM
                     PPKSTWFEPKLRSGLFTHRLD"
     gene            8344..>8612
                     /locus_tag="PLF77_09040"
     CDS             8344..>8612
                     /locus_tag="PLF77_09040"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPC09037.1"
                     /translation="MDTLVFIHGLESTAQGTKGQFFCKNFPQMIIENYTGDFAARMRK
                     LQSLLNDKTDLIIVGSSYGGLMAVQYAIENEHRVKKLILLAPALNL"
BASE COUNT         2338 a         1958 c         2027 g         2189 t
ORIGIN      
        1 gcgccaacat taccggcatg cagaatgata tcggccgcct ggcctggctg gccggtggat
       61 ttccggaatc cgttcccact aacacgctga ccatgcagtg tccttccggt atggcagcca
      121 cgcagcatgc cgcccgcgcc attatgtgcg gagaaggcga tacctacatt gcttccggcg
      181 tggaagacat gcagaaagtt ttcatggcca tgcacatgga tttcccgccc cgtctggcca
      241 accgttataa cattcttgag ctccccatgg gcaataccgc agaaaaggtc gcggaagttt
      301 ataaagtcag ccgtgcagat atggaaacca tggcgtactg gagcaataag aaagccgacg
      361 aagccaccaa ggccggaaaa tttaaaaatg aaatcattcc gatggaagga ttaaaagatg
      421 acggcacacc cttcatggtc gaaaccgacc agtggatccg tggtgacgtt tcgatggaac
      481 agatggcaac catgaaaact cctttcaaac cggatggagt tgttacagca gccacttctt
      541 cgcctttgac aatcggcgcc gcggccattc ttttaatgga acgctccaaa gccgacaaac
      601 tgggactttc ttacagcatt aaatatgaag caggcgcttt aggcggttgc gatcccaccg
      661 tgatgggcat tggtcctatt cccgctgtcc agagacttct ggcgcgcaca ggcaagaaag
      721 tcagcgatat cggtgtatgg gaactcaatg aggcttttgc cagccagtct ctggcatgtc
      781 tgagagaatt gggcattgcc cagaatgctc cttttgacaa tgtaaacatc tggggcggcg
      841 ctctggcgct cggtcatccg ttgggagaaa gcggatgccg cattctggtg accctgcaca
      901 gtattatgaa gactgaccga aaagatgcca aatatggtgt tgcttctctt tgcggcgctt
      961 tcggcaacgc cggcgccacc atgttgagca gagtctaata cgtgatctgc taaacgcatc
     1021 catgaaaaag ggagttcctg atatcaggaa ctccctttct tttttttatg atgaattcta
     1081 tcgaccaatc aatttttaca tttttccgga aagaaacaga tcggcatcgc gggctgtttc
     1141 atcggggatt tcctccatcg gcgtgtgtcc ggcgccttcg tattgtatga attttgctcc
     1201 cggcaattct ttcttccagt tttcgaaata tttaaaagga atccattcat ccctcgttcc
     1261 ccacataacc agcgtcggga cgcggatgtc ttttatgcct tgggagagat tttcatcgcg
     1321 gcacaatttt ctcatttccg tgaaaacatc aatataggat cctttgttgc cttctctcat
     1381 ggctagatca aaatagcggt ctttgagttc ctgggtcact ttggatttgt ctccgaaaac
     1441 ctgttcaacc gcgttgtcga aaagaaaccg cggcatcgta aacctggcga acggtcggat
     1501 aagcggattg ctggcaaaag caaggagtcc cggcagcggc tggggaaatc ccacggaatc
     1561 aaccagaata agcttctcaa ccctgtccgg atgtttcagt gtgtaattcc aggaaatgta
     1621 gcctcccaga gagtttcccg ccagataaaa cttttccagt ttcatttcat caacgacttt
     1681 ttcgaagagc ctgaccgctt cgtctttccg gtacagagat ttatcggggg ccggtccggt
     1741 cagtccgaat cccggaatat ccagccggat gatacgatag cgatctttca gccgctgtgt
     1801 ccatccgtcc caggtgtgca gggacgcaca gaccccatgc aacaggatca gaacaggtcc
     1861 ctgtccctcg tctttataat gaattttcat ttcatcgatt tcgatgattt ctgattctgc
     1921 atcggcatat ttcagtttca gtttttccac cgggacagtg ttccatccca gtgccgtaca
     1981 gcccgacgtg accgccatga tacacaataa caaaatcgga atgagtgctt tgagcgtttt
     2041 cattttttcg gcctttcatc aattgtattt aaacaacgcg aggattttga caagaactat
     2101 agggttttcg attttttatg tcaataaaat atcaaatcgg aaacagaact gaccgggagt
     2161 ccgttcgaaa aacttttttt ataaaaactt attgtcaaat tcattgtcat gctttatatt
     2221 aaaatcaaac attcaaacga attcgagtta gaaaggaggc tgttatgaaa acctatgcca
     2281 taaagtatct cgaactggca gaaaaacacg cggaaaagat agcggcgaga tggttgaaag
     2341 acgtaaaaaa caactctagg acaccgactt ataaaagttt gaatgaagca gaaatcacca
     2401 atcaatgtgt caggttttat caaaccttca gtaaaatgtt tcttgatgaa aaaataacgg
     2461 atgattcatt gcgctatttc cggagttatg ctgaggaaag tcataccatg ggcatccccg
     2521 caaaagaaac gctgtatgct ctcattctga tgagacgcca tatctggctc tatgctgaat
     2581 ttcaggccat ctttacttca gggattgatc aacggcaggc cctcgatacg ctcggacgca
     2641 ccattttatt gttcgattac gcctgttatg aagtaacgaa ggaataccag tcactgacga
     2701 gataacgata aaataaaaaa tatgtcagca attatttagt gccggactta atctcataga
     2761 aaggagaata ttgttatggt cgactctata aagttactcg atattacgga gcaaaacgcc
     2821 gaagaaatcg ccgagcactg ggcgaaggaa gttcataaaa acaagagaac tacccattat
     2881 cgaaacataa aaaaggaaaa actggtcgtt tacgcagtgg atttttaccg gaatttacga
     2941 aaattgctgg ttcccgagga tcgtcttgaa tatacccggg aatattttca gaaatatgcg
     3001 aaaaaatgcc atgaaatcgg tcttcccttg caggaagcga tttacggact tgttctcatg
     3061 cggcgtcaaa tgtggctgta cgccgatttt caggccatct tcatcgatgc cctggaacac
     3121 aatcaggcga tcgacagtat tatgcgaatc atgctcatga tggattatgc ggtttatgag
     3181 atcacccagt attatctgga aattgcaaag cattgatttt ctgaaagata acgtaaaaac
     3241 gacaaaaaaa gtccccggaa tcagatgata tcggccgggg atttttttat ttgataacgg
     3301 aaaagtccct taatttgaaa tcgttaaaat ttatataagt atttgataat gtattgttat
     3361 tatagaaatc tccggaaaaa gtaaaataaa atacttaaat ctcaataata tgttgacaga
     3421 gtgaattttg ggatgtataa cacgcaatac atgactttca gtcatttgat ataattatat
     3481 taaacaatgt aaaagatcgt ccgtttttta cattttcgca tgtcagaaaa attattggga
     3541 aaggtgtcta gagtctcgtc gttcagtcag aacgagtaca aaatacaata aacaaagttt
     3601 aaggaggatt atatggcgag tttgtgggtt gatttacggg atattaaatt ccagttgttt
     3661 gaagtattaa agattgagga aacattgtta ggcaaaggcc gttacaagga ttttgatgca
     3721 gatacatgca tcatggctct tgatgcggca gctaaatttg cagagaacga cattgctccc
     3781 acctatccgg atgaggttca tcgtaaaccg gtcgaggcga cattcgacaa aggcaaagta
     3841 acgacgcccg aagcttacaa gagattatgg aagctttatt gcgaaaacgg ttacatggct
     3901 atggctgacc agccggaagt cggcggacag ggattccccg ctgtcattgc cgctgccgca
     3961 gcaaatatgt ttttggcctg caatcaggct tttgttatgt atcccggtct ttcccacggt
     4021 tgtgcacgat tgatggaaga tttcttccag catccgctgc gcgacatcgt tttggaaaaa
     4081 atgtacaccg gcgaatgggc gggaacgatg tgtctgacgg agccgggcgc cggttctgac
     4141 gtcggagctc tgaagacaac cgccaaaaag aatgctgatg gcacctactc catcgtcggc
     4201 accaagagct tcatttccgc cggcgaccat gatctgaccc cgaacatcat ccatccggtt
     4261 ctggccagaa tcgaaggcga ccccaaggga acaaagggca tttccatttt catggttccc
     4321 aaaatcagat tcaatgaaaa gggcgaactc ggagaaccca atgatgtgca gtgcggcaat
     4381 atcgaaagca aaatgggtat ccacggcaac gccacctgta cactgaactt cggttcggac
     4441 aataagtgca tcggttatct gatgggtgaa gaacgtcagg gtatgccgat catgttccac
     4501 atgatgaacg aagaacgtca gggcgtcggt atgatgggcg cttctctggc aatggccgct
     4561 tatctgcacg ctctggatta ttccaaacag agaattcagg gccaggatgt catcatgatg
     4621 gccatgaagg ttgaagacgc acccgccgtg ccgatcatta aacatccgga tgtccgcaga
     4681 atgctgctca aacagaaagc catttccgaa ggtctgcgcc ttctggcata tttctgctac
     4741 ttctgcatgg ataatgaatt cagcgcaatg atcgatggta acgaggagca gaaagaatac
     4801 tggcatggcc tgatcgaagt tcttacgccg atcgtcaaat cctactgtac agaaaaaggc
     4861 caggacagca tcgaactggc cgtgcagtgc tacggcggtt acggtttctg ccgtgaatac
     4921 cccgtcgaac agatgatgag agataacaag atcaatctga tttatgaagg gaccaacggt
     4981 attcaggctc ttgacctgct gggccgcaaa ctgggcatga agagaggcgc ttattttatg
     5041 aacctgctga gcgttaccaa ggaagctatt gaagaaggca agaaacttga tgctttcaag
     5101 aaggaagccg agaccgtaga gaaagccctg aatgcctgtg ctgaaacggc gctggccttc
     5161 tccaagatga tcaaaaccac gcctttcgtt ccgctgattg gcgcctgcga ttttctgaac
     5221 tgcctgggtg atgcgcttgt cggttggctg cacctcagaa tggccctggt tgcgattccc
     5281 aagttctttg cagcagatac cggtgaaatg gataaagcct tctatcgcgg taagattgaa
     5341 ggtgcaagat tcttcattaa ccgcatcacc ggactggtca ttcctcatct ggaaaacctg
     5401 aaacaggatg aacagagtgc gatgaacatt cctgaagaat catttgctgt ctaaaaaatg
     5461 attttttata aatgaatttc cggaacgggg aggcttgaag cctccccgtt tttttagtga
     5521 cactcgctaa gcgcgaatgt aatgacgcgc gaagcgtcag tggaactatc cccggagcgc
     5581 agctcagcgg gatcagacac tcgctaagcg cgaatataag gaaacgcgaa gtaaagccgc
     5641 attatcctcc cccaagcgaa agccggacgg gattgtcccc ttgacctgtc ttttaattta
     5701 tgtcagaaaa agtaatgcgc aaacgaaaac cgcaaacgag attacaatcg atcagcgaaa
     5761 tcctgttccc gattctgaaa aaaagaggaa tgatttcaaa aatcgaggag aaaacacttc
     5821 agacactctg gccgaaagcg gtgggtcctc aaatcgcctt gcaaacccgg ccggattctc
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cagcagcttc atttcatgaa
     6001 agaagaaatt ctcgttaaat taaataacct ttccgggaaa cccctgatca aagaaatacg
     6061 ttttgtggtc ggtcactcac cggtaaatga aaaagaggct aatcctgttt tgaaggagaa
     6121 gaaagcggtt ttaaaaaaaa gagacagaga catgattgcc gaatgcacgg aagctctgat
     6181 cgaccaggaa ctggccgcgg tattaaaacg cattatgagg ctggaaatca gccggcgtcg
     6241 tcaatgggaa agccggaaag gtcgctgaag acgccggaca ttttctcgga atattttccg
     6301 ggccggtcgt aaatgaacat cggcggttca accttcaact cttctttgga accttttctc
     6361 ccttcgacca gcacaaattc ggccccggaa gatacatcag aaaaaatcag tttcattttt
     6421 ttgggctcaa tgccgtgtaa cctgaacagg taaacaagct ccaccagtcg tttagccgga
     6481 taaatcgtaa aaaccgttcc cgccggtttc aagagatatt ttgccgcgtt taaaaaatct
     6541 ttgagggacc cttggatttc atgacgggca atggctcttt cttccagggg attgatccgg
     6601 ccggaatgta attttcggta aggtgggttg aataccactg cgtcaaacgt ccgttcctgg
     6661 aaaatagttc tgacgttgcg ggcatcgccc caaacgatgt caatgcggtt ttccagttga
     6721 ttgagttgaa cgtttttccg ggccatgacc gcaagacgtt cctgaatctc caagccgacg
     6781 cattgggtgg tcggaaaacg ttttgtcagg atcagcagga tgataccgct gccgcagccg
     6841 atatccatga gccgtgtccg ctttttcaga gaaacgaaat gggccagcag gatggaatcg
     6901 atggaaaaac gatacccttt ttttttctgg aaaatgcgca aagcgccgtg aagaatttca
     6961 tcaagggttt cttcttccat cgttcgaatc atcaatcttt ttcaatccag cctgtgcgtg
     7021 aataatccgc tgcgcagttt gggttcgaac catgtcgact tgggaggcat aattgctccg
     7081 gcatcggcca catttatgat ctgctccatg gtggtgggat aaagagagaa cgccacggca
     7141 aaaccgtcct gattcaccag tttttccagc tcgtccattc ccctgatgcc gccgataaat
     7201 ttgatccggt catccacacg cggatctttg attcccagaa ccggatacaa cagatgctcc
     7261 tgaagaatgg ccgcgtccag actggccacg ggatcgtttt tgtcgtaaac accttccttg
     7321 acggtaagtt tatgccactg gcctcccaga tacatgccga aatcatgaag ctggttcggt
     7381 gatcggtccg aaaaatcttt gctgacaatg aaagcggaac cgattttatc gataaattgg
     7441 tcaggtgtca gaccgtttaa atccttaacg gcacggttat aatccataac tctcagttgc
     7501 gtatcgggga aaaacaccgc cagaaccgat tcgtattccc cggcgaaccc tctggcggat
     7561 tcctttgcac gtcttgtccg ggcaatggcg gctgcggcgg ccgcccggtg gtgaccgtcg
     7621 gcgatataaa gtgcgtccac ggcgagaaac tcttttttta tttcctcaat atgacgggag
     7681 tcggaaataa tccagaccgt atgcgtcacg ccatcatcag aggtaaaatc atattccggc
     7741 atgccgaagg tcacgtgatc gacaatcgta ttaatgcccg gtctttcacg ataactgatg
     7801 aatacgggac cggtctgcgc atttacggtg tcgacatgac ggatgcggtc ttcttctttg
     7861 tcttgccggg tcaattcatg ctttttgatt tttccggcat cgtattcgga agcgttcatc
     7921 acaccgacga ttccggtttg tgcccgcgtg cccattttct gcctgtacac ataaaaatgc
     7981 ggcgattcat cctggatcaa aatgccctta acacgcatat cggtgaaatt ccgtttggcc
     8041 gtctgataga taagagggtc atcgcttttc gttttgtcgg ggacgtcgat ttccgatttt
     8101 tccacatgca tgaaactcaa cgggttgccg gaaaccgctt ttttcccttc ttcccgggtc
     8161 attacatcgt acgggagagc cgccacctga gcgacaaatt ttttatccgg tcgaaccgct
     8221 ttaaaaggta atactattgc cataatttga tctcctgaca gcaaatcttt tcttgcggaa
     8281 tggataacat actgacataa tctatacaac ataaaaatat gccggagaag ggcagggata
     8341 cgcatggata cgctggtttt tattcacggg ctggagagca cggcgcaggg caccaagggg
     8401 cagtttttct gtaaaaattt tccgcagatg atcattgaaa attatacggg agattttgcg
     8461 gcgcgtatgc gaaaacttca gagccttctt aacgataaaa ccgatttgat catcgtcggt
     8521 tcgagttatg gagggttaat ggccgttcaa tacgccatcg aaaatgaaca ccgggtaaaa
     8581 aagctgattc ttctggcgcc ggcgttgaat ct
//
LOCUS       DAOOLB010000082         8436 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_9866_length_8436_cov_33.188999, whole genome shotgun sequence.
ACCESSION   DAOOLB010000082 DAOOLB010000000
VERSION     DAOOLB010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8436
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9866_length_8436_cov_33.188999"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="PLF77_09045"
     CDS             <1..710
                     /locus_tag="PLF77_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HPC09038.1"
                     /translation="YGSVFSSEHLLPALWFHPTANIMTLFAATILIGILFIMTGLLVN
                     IINNILNADYTEALLDKRGLVILVIYAATVLFAFNYQRSGQVPAIWEISAFIMLPLTL
                     FALRGVIGPAFFHQESPHSASEYIIETVMEIVETGLSLFANTISFIRVGAFALSHAGL
                     SIVTYTLAGIADPGLQSAGAILIIITGNIFIIGFEGLICGIQSMRLEYYEFFSKFFQG
                     DGVVFSPFTLKAQASEV"
     gene            712..1047
                     /locus_tag="PLF77_09050"
     CDS             712..1047
                     /locus_tag="PLF77_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HPC09039.1"
                     /translation="MIKNKLFKHFVFAVFTGFIWFALLPTVINAAENVQVNSGANWMG
                     LAYIGAGLAVGLACIASGFAVARIGSAAIGAVSEKPELMGRTLVFLGLAEGIAIYGLI
                     IAIMILNKL"
     gene            1260..1565
                     /locus_tag="PLF77_09055"
     CDS             1260..1565
                     /locus_tag="PLF77_09055"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HPC09040.1"
                     /translation="MEKIYIIGDMHTVSAFRLAGVEGMVSTADSASVRLEEIIKKRDA
                     GIVLMTNDLAAALQERIREINLNRQNPVVIEIPGMDDIGGLRMSAMNYISEALGIRL"
     gene            1575..2180
                     /locus_tag="PLF77_09060"
     CDS             1575..2180
                     /locus_tag="PLF77_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09041.1"
                     /translation="MDNTTLENSIREEASRAITAIREKEALEMRQMDEVFSMEMEDFR
                     RQAEAETESHIRQEISKLENKALLERKKMKLQNAEQFINRIVDEVMKNIRNDRLYKQF
                     LLEAIRDGVKISGADVDIKLSNEDIFLKKDIVAAIAAMDGRKKVSITEDSKVQWGGCL
                     IRDKETGRIFNHTLERIYFRKSLLIRQQVMSLLMRKAEGNQ"
     gene            2206..3969
                     /locus_tag="PLF77_09065"
     CDS             2206..3969
                     /locus_tag="PLF77_09065"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HPC09042.1"
                     /translation="MDEILKGNVISVNGPIVKVVGLTGIKMFDIAEVGQERLIGEVIR
                     LAGEITIVQVYEDNTGLKPGDDVISYGRPLSVLLGPGLIANIYDGIQRPLVGISEVCG
                     SYIKKGIKLPPLDVNKKWDFQPLVKTGEDINEGTLVGEIKESPLVVHRVLVPPGIAGR
                     LTAIVNAGPYTIEDDIFEIDTGYGKYNGKLAAYWPVRKPRPSRNRKKPFMPLITGQRI
                     IDTFFPIAKGGTAAIPGGFGTGKTMTQHALAKWCNADIIVYIGCGERGNEMTDVLTDF
                     PKLIDERNGRPLIERTVMIANTSNMPVPAREVSIYTGVTIAEYYRDMGYSVAVMADST
                     SRWAEALRELSSRLGDMPAEEGFPPYLGTRLAEFYERAGLVETLSGSNGDITIIGAVS
                     PPGGDFSEPVTQHTSRFVRCFWALDKILADARHYPSISWIDSYTEYLVDIEDWWKNLD
                     SEWLTTRNEVMNILLEDHRLEQVVKLVGSDALPPSEQYILFCAEMIKNAFLQQNSFDS
                     QDKFCSPEKQLKLLKCLLVLYQKGMTLIQEGVPVKDISQSDVVSEMVRLKSAVPNKEL
                     VEIDDYLKRLEQELMSIKSSS"
     gene            3980..5371
                     /locus_tag="PLF77_09070"
     CDS             3980..5371
                     /locus_tag="PLF77_09070"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HPC09043.1"
                     /translation="MTMEIEYTNLSGISGPLIFVDGIKGVGYGDIVEIRDASGDLRIG
                     QTLEVDEQRAVVQVFEGTSGLSVKETRVTFVGRALEIPVDREMLGRVFDGLGRPVDGA
                     PQVITDRMMNVNGLPINPWSREYPRDFIQTGISAIDGMNTLIRGQKLPIFSGSGMPHN
                     RIAAQIARQARILTAEEEFTVIFAAMGVKFDVARFFIDSLEESGVLTHSAIFLSLADS
                     PSIERLVTPRSALTLAEYLAFEEGMHVLVILTDMTNYCEALRELSSSRGEIPSRKGYP
                     GYLYSDLASVYERAGRLKGGKGSITQMPILTMPSDDISHPVPDLTGYITEGQIVLERE
                     LFNKGIYPPIAGLPSLSRLMKDGIGEGRTRKDHGQVAAQLFASYAKVKRIRALAAIVG
                     EEELSDLDKIYLKFGTAFEEAFLRQAYHENRSIEQTLEIGWKMLSLLPRDELYRIARE
                     DIQRYYMSETEKT"
     gene            5371..5991
                     /locus_tag="PLF77_09075"
     CDS             5371..5991
                     /locus_tag="PLF77_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HPC09044.1"
                     /translation="MPKIELPPTKSSLRKLKEDLAFAYEGYDLLNQKREILAIEIVRK
                     IDVIRKIEAEFQDIVNRLYLAYRQAAVDMGSEAVTLQSCSEMRSYYLHMNLTKLMGLK
                     LLNFTLDLKNNLPAFTLPPTTSAYDNAKEQSRKALHIVVQYATIMKSVIMLSRELKKV
                     QRRVNALEKIFIPQHEEAKKYITDRIEEMEREEIFVKKLIRQRQQR"
     gene            6030..6554
                     /locus_tag="PLF77_09080"
     CDS             6030..6554
                     /locus_tag="PLF77_09080"
                     /inference="COORDINATES: protein motif:HMM:NF025719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsbRD N-terminal domain-containing protein"
                     /protein_id="HPC09045.1"
                     /translation="MKSKSDVLKYLELAERQARDKKKKSNMKTYAYKYLELAEKHAEK
                     IAARWVKDVRNNTRTQTYKKLNEKQLVDQCTEFYQQFSKMFIYEKITDDVTKYFQKYA
                     LECYALDIPAKEAIYALILMRRNIWLYAEFQMIFNSAIAKQESLDTLNRTILLFDYAV
                     YEVTREYLELMKTT"
     gene            complement(6892..>8436)
                     /locus_tag="PLF77_09085"
     CDS             complement(6892..>8436)
                     /locus_tag="PLF77_09085"
                     /inference="COORDINATES: protein motif:HMM:NF023326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HPC09046.1"
                     /translation="REEDLIAREPDAEEISQYPDRIKIGDAAFNCRYNFEPGKNDDGV
                     TVKVPLGFISKAATANIDRHLPSLLKEKAVHLLKSLPKSIRLKLPPPAKIVELLVEDK
                     SNLDKPLPQALSQLLLDKYKIKVPKDDWATEKLPDYLNVRYSVIDEKGNEIKESRDIH
                     LLQKELTGTVSTSAMDKIRGEWEKEGITGWDFGDLPQQIPLRGTHGLIGYAYPALTIS
                     DDSIDLRLFADEKQSAFHHLHGIAALYDIHFADKMKQLKKNVALTGAMKTSAAGIGPP
                     KQLEQSIINRVRKDLFYKPWRRQEEFIAHAGCLNSQILPYGQQVLIAIEPVLKACADT
                     CTVIGKLMTKNRNNNPVLNFIKDIQTEISRLVPVDFPELYTFERMKELPRYCKALALR
                     AERGNLNLSAAQRKMQDVLIYSQKLQQMVNGRVQPSLFDLAVVKKQSSPKDPTEPKGT
                     GFKEIILLKENITIDCPDEKKTMVEEFFWMIEEYKVSLFAQELKTPYPVSPKKLNQLM
                     EDIEKY"
BASE COUNT         2303 a         1828 c         2105 g         2200 t
ORIGIN      
        1 tttatggcag cgttttttcc agcgaacacc tgcttccagc cctttggttt cacccgactg
       61 caaacattat gacactgttt gccgcaacga ttctcatagg cattttgttt atcatgaccg
      121 gtcttcttgt gaacatcatc aataatatcc tgaatgcgga ttacacggaa gcccttctgg
      181 ataagcgggg actggtgatt ctggtaattt atgccgctac tgtccttttt gcgttcaatt
      241 atcaaaggtc cggtcaggtt cctgcaatat gggagatcag cgcttttata atgctgcccc
      301 tgacattgtt tgcactcaga ggcgtcattg gcccggcatt ttttcatcaa gagagcccgc
      361 acagtgcttc agaatatatc atagaaaccg tcatggaaat tgtggaaacc gggctgagtc
      421 tgtttgccaa cacgatatca tttatcagag tcggtgcttt tgctctttca catgcggggt
      481 tgagtatcgt gacctacacg cttgccggaa ttgccgaccc cggtttgcaa tcagcgggtg
      541 cgatcttaat catcattacc ggtaatattt ttatcatcgg ttttgaaggt ttaatttgcg
      601 gcattcaatc catgcgtctt gaatattatg aattcttcag caaatttttc cagggagatg
      661 gagtcgtctt ttctccattt accttaaagg cacaagcatc ggaggtgtaa gatgatcaag
      721 aataaattgt tcaagcattt tgtgtttgca gtcttcaccg gtttcatttg gttcgcattg
      781 ttgcccaccg tcattaatgc agccgagaat gttcaggtta actccggagc aaactggatg
      841 ggtttggctt acatcggtgc ggggctggct gtggggttgg cctgtatcgc cagcggcttt
      901 gccgtagcac ggatagggtc tgccgccatt ggcgcagtga gtgaaaagcc ggaactcatg
      961 ggaagaacgc tcgtttttct cgggctggcg gaaggcattg cgatttacgg tttgattatt
     1021 gcgatcatga ttttaaataa actgtaatta cattttcaaa tcaggatgat accgcggcgg
     1081 cagtgaaact taaattacgg aaacgaagtg aaccgaaatt aaatggttga actgtcccgc
     1141 ttcagcgggg caaggaggag gctccgcagg cgtattgcta acccagtgac ctttaggtta
     1201 tacgtcgagg aagccgacga cgccaacaaa ggtagcgctt gacttggaaa gggaatgtca
     1261 tggaaaagat atacatcatc ggagacatgc atacggtcag cgcgtttcgc cttgccgggg
     1321 tggaagggat ggtctcaacg gcagacagtg catcggtgcg actcgaagaa attataaaaa
     1381 aaagagatgc cggtatcgtc ttgatgacga atgaccttgc tgcagcatta caggagcgta
     1441 tcagagagat taacctcaat aggcagaacc ctgtggtgat agaaattccg ggcatggatg
     1501 atatcggggg gctgcgtatg tcggcaatga attatatttc cgaggctctc ggtattcgtt
     1561 tgtaaggata gagcatggac aatacaacat tggaaaattc tatccgtgaa gaagcctctc
     1621 gcgcaataac cgccatcagg gaaaaagagg ctctggagat gaggcagatg gatgaggtct
     1681 tttcgatgga gatggaggac ttccggaggc aggctgaagc ggaaacagaa agtcacatcc
     1741 ggcaggaaat ttctaaactg gaaaataaag cgcttctcga gcgaaaaaag atgaagcttc
     1801 agaatgcaga gcaatttatc aatcgcattg tcgatgaggt catgaaaaac attcgtaacg
     1861 atcgccttta caaacaattt cttctggaag caatccgcga tggcgtaaaa atatcgggtg
     1921 ctgatgtaga tataaaactc agcaacgagg atattttcct gaagaaagat atcgtcgcag
     1981 ccatcgcggc tatggacgga agaaagaaag tttccatcac ggaagattcg aaagtccaat
     2041 ggggcggatg tctcatccgg gataaggaaa caggtcgtat ttttaatcat acgctggaaa
     2101 gaatctattt cagaaaatct ctgctgatca ggcagcaagt catgagttta ctgatgcgga
     2161 aagcggaagg aaatcaatga cgcagttaac ttttaaggag cgtatgtgga cgaaatatta
     2221 aaaggaaatg tgatttcggt gaatgggcct attgtcaagg ttgtcgggct gaccggaata
     2281 aaaatgttcg acattgccga agtgggacag gaacgactga tcggagaagt catcaggctt
     2341 gccggagaaa tcacgatagt ccaggtgtat gaggataaca caggcttgaa gccgggtgac
     2401 gacgtcattt catacggacg tcctctttct gtgctcctgg ggccgggact gatagccaat
     2461 atttacgatg gtattcaacg gcctctcgtc ggtatttccg aggtatgcgg aagctatatt
     2521 aaaaaaggca ttaaacttcc gcccctcgat gtcaataaaa aatgggactt ccagccactg
     2581 gtcaaaaccg gtgaggatat aaacgagggg actctggttg gagagattaa agaaagcccg
     2641 cttgttgtac atcgggtgct tgttccgccg ggcattgcag ggagattgac tgctatcgtg
     2701 aatgccgggc cttacacgat agaagacgat atctttgaaa tcgatacagg ttacggcaaa
     2761 tacaacggga aacttgccgc ttattggccg gtgcgcaagc cccgtcccag ccgaaacaga
     2821 aaaaaacctt ttatgccgtt aattacaggc cagagaatta tcgatacgtt tttccccatt
     2881 gccaaaggag gaacggcagc cattcccggc ggctttggca cgggaaaaac catgacacag
     2941 catgctctgg caaaatggtg taacgccgat attatcgttt atatcggctg tggcgaacgc
     3001 ggcaatgaga tgacagatgt tctcacagac ttcccgaaac ttattgatga acgcaacggc
     3061 cggcctctga tcgaacgaac cgtgatgatt gccaacacat ccaatatgcc ggttcccgcc
     3121 cgcgaagtaa gcatctatac cggcgtgacg attgcggagt actaccgcga tatgggctac
     3181 agcgtggcag tcatggccga ttccacatca cgctgggcgg aagcgctgcg ggaactttcc
     3241 agcaggctgg gcgatatgcc ggccgaagaa ggattcccgc cttatttggg tacacggttg
     3301 gccgaatttt acgaaagggc cggtcttgtg gaaacgctga gcggcagtaa cggcgacatt
     3361 accatcatcg gagcggtttc tcctccgggc ggagattttt cggaaccggt cacgcaacac
     3421 acatcaagat ttgtgagatg cttctgggcg cttgataaaa ttttagccga cgccaggcat
     3481 tacccgtcga ttagctggat cgacagttat acggaatacc ttgtcgatat tgaagattgg
     3541 tggaaaaatc tcgacagcga atggctgaca accagaaatg aggtcatgaa tattcttttg
     3601 gaagaccatc gtttggagca ggtcgtgaag cttgtcggca gtgacgcatt gccgccttct
     3661 gaacagtata ttctcttctg cgccgaaatg atcaagaatg ccttcctgca gcagaattct
     3721 ttcgattcgc aggacaagtt ctgcagtccg gaaaagcaac tcaaactttt aaagtgcctt
     3781 ttggtcctct accaaaaagg catgacgctg attcaagaag gtgtgcccgt caaagatatc
     3841 agtcaatcgg acgttgtttc ggaaatggta cggcttaagt cggcagttcc caataaagaa
     3901 cttgttgaga ttgacgatta cttgaaaaga ctggaacagg aattgatgtc gataaaatcg
     3961 tcatcgtgag gattgtaaaa tgaccatgga aattgaatat acaaaccttt ccggaatttc
     4021 cgggcccctg attttcgtcg acggaatcaa aggcgtcgga tacggcgaca ttgtagaaat
     4081 ccgcgatgct tcaggagatt tacgtatcgg tcagacgctg gaggtggatg aacagagagc
     4141 cgttgttcag gtcttcgagg ggacaagcgg cctttcggtg aaagaaacaa gagtcacctt
     4201 tgtgggaagg gcgttggaaa tacctgttga tcgtgaaatg ctgggcagag tttttgacgg
     4261 cctgggcaga cctgttgatg gcgctccgca ggtcatcaca gacaggatga tgaatgtcaa
     4321 cggtctgccg attaatccct ggagtagaga atatcccaga gatttcatac aaaccgggat
     4381 atcggcgatt gacggcatga acacgctgat ccgcggccag aaactcccga ttttctcggg
     4441 aagcggtatg ccgcacaatc gtattgccgc ccagatagcc aggcaggcaa gaattctgac
     4501 tgcagaagag gagttcaccg ttatctttgc tgcgatggga gtcaaatttg acgtagccag
     4561 attcttcatc gattccctcg aagagtcagg agttctcacg cattccgcaa tattcctgag
     4621 tcttgccgac tcaccatcca tcgaacggct cgtaacgcct cgttcagcgc taacgcttgc
     4681 tgagtatttg gccttcgaag aagggatgca tgtactggtg attttaacgg atatgacaaa
     4741 ctactgcgaa gccttgaggg aactctccag ttcgcgtggt gagattccgt cacgaaaagg
     4801 ttatcccggc tatctttaca gcgatttggc ttcagtctat gaacgtgccg gtcgactcaa
     4861 aggcggtaaa ggctccatta cccagatgcc gattctgacc atgcccagcg acgacatttc
     4921 ccatcctgtt ccggatctga cagggtatat caccgaaggt cagattgtgc tggaaagaga
     4981 gctgtttaat aaaggaatct atccgccgat tgccggcctc ccttcccttt cccggctgat
     5041 gaaagacggt atcggtgaag gtcgaacacg caaggaccat ggccaggttg ccgcgcagct
     5101 ctttgcctcg tatgctaaag tcaaacgcat aagagcgttg gcggccatcg tgggagagga
     5161 ggaactttcc gatctggaca aaatttactt gaaattcgga accgcttttg aggaggcttt
     5221 tttgcgtcag gcttatcatg agaatcgttc cattgaacag accctggaaa ttggttggaa
     5281 gatgctttct ctgcttcccc gtgatgaatt gtatcgcatt gctagagagg atatccagcg
     5341 atactatatg tctgagacgg agaaaacgta atgccgaaaa tagaattacc cccgacaaaa
     5401 tcatcgctgc gaaaactcaa ggaagatctg gctttcgctt acgaaggcta tgatctgctt
     5461 aatcagaaac gggaaattct ggctattgaa atcgttcgga agattgatgt catccggaaa
     5521 attgaagccg aatttcagga tattgtaaat cgtttatatt tggcttatcg acaggctgcc
     5581 gtcgatatgg gttccgaagc tgttacatta caaagttgtt ccgaaatgag aagctattat
     5641 cttcatatga atttaacaaa actgatggga ctgaaactgt taaatttcac tcttgatctc
     5701 aagaacaatc ttcccgcttt tactttgccg ccaacaacat ctgcttatga taacgcaaaa
     5761 gaacaatctc ggaaagctct tcacatcgtg gtgcaatacg cgacaataat gaaatccgtc
     5821 atcatgcttt caagagaact gaaaaaagtg caacggcggg tgaacgctct ggagaagatc
     5881 tttattccac aacatgagga ggctaaaaaa tacatcaccg acaggattga ggaaatggaa
     5941 cgcgaggaaa tattcgtgaa aaaacttatt cgacagcgcc aacaacgcta aatcaattcc
     6001 ttcagtccgc taataaaagg agatgaacta tgaaatcgaa atcagatgtt ttgaaatatt
     6061 tggagctggc agaaagacaa gcccgggaca agaaaaagaa atcaaacatg aaaacatacg
     6121 cctataaata tctggaactg gctgaaaaac acgcggagaa gattgccgca agatgggtaa
     6181 aggacgttag aaacaacacc cgaacgcaga cttataaaaa gctgaatgaa aagcaattag
     6241 tcgatcagtg tacagagttt tatcagcaat tcagtaagat gttcatttat gaaaaaatta
     6301 cggatgacgt gacgaagtat tttcagaaat acgcactgga atgttatgct cttgacattc
     6361 ccgcgaaaga agctatttac gcgctgatat tgatgcgacg gaatatctgg ctatacgcgg
     6421 aatttcaaat gatcttcaac tcagctattg ccaagcagga atccctggat accctcaacc
     6481 gaactatcct gctgtttgat tacgcggtgt atgaggtcac cagggaatac ctggaattga
     6541 tgaaaactac ttagcggtcg aaattcatta ttgctgacgg tgaatcatgc catcgattag
     6601 gcacgggtgc atgaggtgct ggtttatgcc tggtgacttc cgcaacccta gaaacgtttt
     6661 tttggcagtg tctcgaatcg tcattgcttt gccattagag aatcggtggc taagattatg
     6721 acaatatgtt ctgctaggag aaggcattct taccgcagtt tatctggcag taaaaaatta
     6781 atgataaaat cgaactaagg cgcataagct cagtcgggtc ttttggactc gactgttttt
     6841 taacgacagc caagtcaaaa agacttggct gaacaaggat taaataaatc actaatattt
     6901 ttcaatgtct tccatcaact gattcaattt cttcggcgag acggggtagg gggttttaag
     6961 ctcctgagcg aacagagaga ccttgtattc ttctatcatc cagaagaatt cttcaaccat
     7021 tgttttcttt tcatccggac aatctatggt aatgttttct tttaaaagaa taatttcttt
     7081 aaatccggtt cctttaggct cagtcgggtc ttttggactc gactgttttt taacgacagc
     7141 caagtcaaaa agacttggct ggacacggcc atttaccatc tgctgcagtt tttgcgaata
     7201 aattaaaacg tcctgcattt tcctttgcgc cgcggaaaga tttaaatttc cccgttcggc
     7261 tcgcagagca agcgccttgc agtagcgcgg cagttccttc atccgttcaa aggtataaag
     7321 ctcggggaaa tcgacgggca caagacggga gatctctgtt tggatgtctt ttataaaatt
     7381 caaaacagga ttgttgtttc tatttttcgt catgagtttg ccgataacgg tacaggtatc
     7441 ggcacaggct ttcaataccg gttcgatcgc tatcaatacc tgctggccat aaggcagaat
     7501 ttgggaattc agacaaccgg cgtgcgcgat aaattcctcc tgtcttcgcc acggcttgta
     7561 aaataaatct ttcctgaccc ggttaatgat ggattgttcc agttgtttgg gagggccgat
     7621 gccggcagcg ctggtcttca ttgcgccagt caaagcgaca ttctttttaa gttgtttcat
     7681 cttgtcggcg aagtgaatgt cgtagagcgc cgcaataccg tgcaaatgat ggaaggcgct
     7741 ttgtttttcg tcggcgaaaa gccgcagatc aatggaatca tcgctaatcg tcagcgccgg
     7801 ataggcgtaa ccgatcaagc cgtgagttcc ccgaagggga atctgctggg gcaagtcccc
     7861 gaaatcccag ccagtgattc cctctttttc ccactcacct ctgattttat ccatggccga
     7921 tgtactgacc gtaccggtca gttctttttg cagcagatga atgtcccggc tttctttaat
     7981 ttcgtttcct ttttcatcaa tgacgctgta gcggacattc agataatccg gcagtttttc
     8041 cgttgcccaa tcatccttag gaacctttat cttgtatttg tcgagaagca actggctgag
     8101 cgcctgaggc aaaggcttgt ccaaattcga tttatcttcc accaaaagct caacaatttt
     8161 agcgggcggc ggcaatttga ggcgaatgga tttaggaagc gacttcaata agtgaacggc
     8221 cttttccttt aataacgaag gcagatggcg atcgatgttt gctgtcgcgg ctttggaaat
     8281 aaaacccaga ggcactttaa cggtgacgcc gtcatcattt tttcccggtt caaaattata
     8341 gcggcagttg aaagcggcat cgccgatttt tatccgatcg ggatattgcg atatttcttc
     8401 agcatccggt tcacgggcaa tcaggtcctc ttcccg
//
LOCUS       DAOOLB010000083         8330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_10046_length_8330_cov_44.106949, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000083 DAOOLB010000000
VERSION     DAOOLB010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8330
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10046_length_8330_cov_44.106949"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1356
                     /locus_tag="PLF77_09090"
     CDS             49..1356
                     /locus_tag="PLF77_09090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HPC09047.1"
                     /translation="MSQQIFSLSSWPRAIVHIDGDAFFTSCEEAIHPELRGKALITGG
                     ERGIVACASYPAKKLGIKRGVPLAEARKICPGLIVLPSDYETYSLFSQRMFNIMRRFT
                     PDVEEYSIDEAFADLTGMRRLLRSSYEEIVLKMKKEIERELGITVSVGLSITKVLAKI
                     ASKHQKPSGVTIIKGRDIASCLGNLPVEKIWGIGPATTNYLAKMGIRYALEFAQLPEK
                     KVREKFTKPGVEIWQELRGESVYPVTAEEKSSYRSISKTKTFAPPTSNADYLFAHLMR
                     NMESACIKARRYCLAPKKIVVFLKKNDFNTVGSEVKLSRPCAFPMEFSGVIRDLFDQC
                     YSVQDIYRATGVVLCELEPDEQVQYSLFDNPLQAEKIRSVYNVADELGRKFGKHALHL
                     GSSHPIDTLGRGRRGNPTVREQTRLKGETRRRHLSLPLLHVKT"
     gene            complement(1388..1948)
                     /locus_tag="PLF77_09095"
     CDS             complement(1388..1948)
                     /locus_tag="PLF77_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09048.1"
                     /translation="MNVKGTVFLTGKVAITANFGEARWNEFMAKLAEKDPFFKNVIMS
                     VTLVPVDKHLFFLDEMLKEFFRNDKSQFLLFGRVAAKFALSPGGPYHSYLLTKDIKQF
                     VESGMPKLWSTYYDGGKFTAKLENNIVHLKISDIPIKHIYFEYLIMGYFKQALKIFGK
                     ENIEKRVRSIASGDDDIYYQYEIKNL"
     gene            2305..>2809
                     /locus_tag="PLF77_09100"
     CDS             2305..>2809
                     /locus_tag="PLF77_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HPC09049.1"
                     /translation="MTLKDIKKVLILGAGTMGQQIGFLCAMHGYNVTMYDISEDILKA
                     SVARMEKLGSSWFVRINRITPEQLKEIMSRITATSDAKTAARNADLISESVPEDPKLK
                     GKIFAQFNTLCPKRTIFTTNTSLLMPSKFAEATGRPEKFAALHFHDVRTTNVVDVMPH
                     PGTDKAVT"
     assembly_gap    2810..2909
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3101..3427
                     /locus_tag="PLF77_09105"
     CDS             3101..3427
                     /locus_tag="PLF77_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09050.1"
                     /translation="MAEDDDVLTTDDNESPVSGYLSIVLTIIGLVMLLLIKILGITSS
                     DETGWSIEFVLYLFISMTFAIAGGFLGFLGLSEDNNKKIFPIIGLIVAAIYVVSLGYV
                     IATSIS"
     gene            complement(3640..4611)
                     /locus_tag="PLF77_09110"
     CDS             complement(3640..4611)
                     /locus_tag="PLF77_09110"
                     /inference="COORDINATES: protein motif:HMM:NF021550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2275 domain-containing protein"
                     /protein_id="HPC09051.1"
                     /translation="MKKCKDIENNLSLYLDDVLSKEDKAAVDEHLRSCRQCEEALARL
                     RKTKELTNSLHEVEPPPWFRKKIMARVREEAGKKSFLYKFFYPLQIKIPVQIFATVCI
                     AVLAVYLYRAGENRFQEVVPSSVPAPVLQTQQDQSPEAKENTSLAERTQPEPNTISQK
                     DKPRESVRKKEVHRVTDVHETDVNELKASDGRDDSNKVAPAQVPEQFSVESAQTKDRH
                     VSGTTLKASVAPRAQDSHIKPGIVLKVTDLEVAVREAEQIFRQCEAKNITATIMPDKA
                     FLTGELNRHNIKDLLRQLSTIGRVEEKHIPAADAEGNTALVVEITNR"
     gene            complement(4608..5228)
                     /locus_tag="PLF77_09115"
     CDS             complement(4608..5228)
                     /locus_tag="PLF77_09115"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPC09052.1"
                     /translation="MSNIINTRAKTAKDEDLKYVTLCQKGDTESFDVLVERHQKKMLN
                     IAYRMIGDYDEACDVTQEAFVAAFKSIRQFKAEAKFSTWLYRIVVNYSKNRLKQLSGR
                     SRREGFSIDATGGRNADQLSDQGMSQESNPGVQMEKREREAYVQKCINTLDAEHRDVL
                     ILRDIQGFSYEEISAILKVPDGTVKSRLSRARNALKDCLQKVIGDL"
     gene            complement(5334..6095)
                     /locus_tag="PLF77_09120"
     CDS             complement(5334..6095)
                     /locus_tag="PLF77_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09053.1"
                     /translation="MAIGILAALIFYHFTKPPRQATPKPVPAAPAAADSPAALPPVQV
                     FTPSDAPPPPEAIPPLPTRQASPKPDKMKMVEQIAAEFHKGHTYTLEGEFVCLDMAID
                     VWNQLVTNGIEAKIMGGTLHESITAWNFRQLAMGSNHAWVVATVGPMEKVAIETTSGT
                     VIKPGMKEYASYFKGIEFDSPAQIKRFELLRRKMNDVCREAHQMIQDWNQNVAGKQLH
                     PAEIVARQSRLEQRKQDCENTFRELREFESKAIFY"
     gene            complement(6296..6766)
                     /locus_tag="PLF77_09125"
     CDS             complement(6296..6766)
                     /locus_tag="PLF77_09125"
                     /inference="COORDINATES: protein motif:HMM:NF008675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HPC09054.1"
                     /translation="MTMDAVPIDVILEQLKIVYEEKMPFNKYLGLKIDSIKADKAVVR
                     IDMRDDLIGNFEKNILHGGVISTILDFTGSVIAQIHIINEMKDAPLEKIVKRLTGMGT
                     IDMRIDYMRPGKGSYFLATGHLLRLGHKVAVARTEFVNDSGELIAVGTSTYIVG"
     gene            complement(6804..8093)
                     /locus_tag="PLF77_09130"
     CDS             complement(6804..8093)
                     /locus_tag="PLF77_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HPC09055.1"
                     /translation="MSINVKKNSKTWVVYILRCCDGSLYTGITNNIEKRLAAHHQGKA
                     AKYTRSRRPVELLVTGTAMERSAAMRLEIKIKRQPKDKKVSALKKHAPRMSSTAATTS
                     KRRVVAAGEHSLFGKETLKAVAGLWQVPLKKIHRKIPVQGSPERSAFRVVLEDTDKRF
                     FVLEQIASQSAAGRKQIAAILDLLAAKKLPQINPYLTDERGNYLLEHQNNFWQLSPFV
                     PGVELDREKYVFEPWRGIVLADFLIALRRKSQKMTLAGSRGVFSLRNYIYKLVREINL
                     YNKDIKNDIGSVVDFLEKDFLPVYEELPVAFCHGDYHPLNIIWSTDGIRCVIDWEFCG
                     LKSELYDVANLIGCIGMENPRSLAGGLVGHFIDRMKRSGIMSQISWRYLTEFIIALRF
                     AWLAEWLRRKDRNMIRLERDYMKLLMDNKTVLQKAWL"
BASE COUNT         1944 a         1982 c         1972 g         2332 t
ORIGIN      
        1 gtttttccgg gtttttcaat ttttcgtttt tcctgtttaa aaggttttat gtctcagcaa
       61 atattcagtc tttcctcctg gccgcgcgcg attgtgcaca ttgacggcga tgcttttttc
      121 acgtcctgcg aggaagccat tcatccggag ctccggggca aagcgctgat tacaggcggc
      181 gagcgcggta ttgtcgcttg tgccagttat ccggccaaaa aactgggcat caagcgcggc
      241 gtgcctctgg ctgaggcgcg taaaatctgc ccgggattga ttgttctgcc ttcagactac
      301 gaaacctaca gcttgttttc gcagcggatg ttcaacatca tgcggcgttt cacgcctgac
      361 gtggaagaat attccatcga tgaggcgttt gccgatctta ccggcatgcg acgattgctg
      421 cgttcctcct acgaggaaat tgtactgaag atgaaaaagg aaatcgaacg cgaactgggc
      481 atcacggttt ctgtcgggtt gagtatcacc aaggtgttgg cgaaaattgc ctccaaacat
      541 cagaagccat ccggggtgac catcatcaag ggaagggaca ttgcctcttg tcttggaaat
      601 cttcccgtgg agaaaatctg gggcatcggt cccgccacaa ccaactatct ggcgaagatg
      661 ggcatccgct acgcgttgga atttgcacaa ctgccggaaa agaaagtgcg ggaaaaattt
      721 accaagccgg gcgtggaaat ctggcaggaa ttgcgcgggg aatcggttta tccggtgacg
      781 gcagaggaga aaagttctta tcgttccatc agcaagacga aaacctttgc gccgccgacg
      841 tccaatgccg actatctgtt cgcgcacctc atgcgcaata tggaatcggc gtgtatcaag
      901 gcccggcgct actgcctggc gccgaagaaa attgttgtct ttctcaagaa aaacgatttc
      961 aacaccgtcg gcagtgaagt caagctgtca cgcccctgtg cttttcccat ggagttttcc
     1021 ggcgttatcc gtgacctgtt tgatcaatgc tactcggtac aggatatcta ccgcgccacc
     1081 ggtgtcgttt tgtgcgagct ggaaccggac gagcaggtgc aatattctct ttttgacaat
     1141 cctctgcagg cggaaaaaat cagaagcgtt tacaatgttg ccgatgaact ggggaggaag
     1201 ttcggcaaac atgcgctgca tctgggaagc tctcatccca tcgatacgct ggggagaggc
     1261 cgccggggta atccgaccgt ccgtgaacaa acccggctaa aaggcgaaac ccgccgccgt
     1321 catttgagtc tgcctctgct gcacgtcaag acatagagaa ttttatgtga acgtaacgga
     1381 ttttttatca gaggttcttt atctcgtact gataataaat gtcgtcatca ccgctggcga
     1441 tacttctcac ccttttctca atattttctt tcccgaatat tttaagggct tgcttaaagt
     1501 accccatgat gaggtactcg aaataaatat gcttgatggg aatatcgctg attttaagat
     1561 gaacgatgtt attttccaac tttgcggtaa acttgccgcc gtcataatag gtggaccaaa
     1621 gtttgggcat acccgattcg acgaactgtt tgatgtcttt tgtgagaaga taagaatgat
     1681 agggcccgcc gggagacagg gcgaattttg ccgctaccct cccaaacagt aaaaactggc
     1741 ttttatcgtt tctgaagaac tctttcagca tttcgtcaag aaaaaaaaga tgcttgtcta
     1801 ccggaacaag cgtgacagac atgataacat ttttgaaaaa cggatccttt tccgcaagct
     1861 tggccatgaa ttcattccag cgcgcttcgc cgaaatttgc tgtgatggcg actttccctg
     1921 tcagaaaaac agtacctttt acattcatat taaatcctcc ccggagtcgt tgattggctt
     1981 aaaaataaga tgagtgatgg tcacgaatat gtaatggcgt tttttcatcg gaaacaatta
     2041 attttcgcat catgccggat tcttacgctt tactgttgca tcaaaggctg cgccgctgga
     2101 cgtcttgccc gtgacagaaa ccgtactttt gcgttcatgt caccttccaa agtatatgtg
     2161 tatgagttaa tccttatatt ttgtgttgca taaatattca agtacaatat tattttttcc
     2221 ccatggcgtc ttcacgtttt ccccttttga aaaacataac cccatgatat tataatttag
     2281 atttatttga agaaaggaaa atttatgaca ttaaaagaca ttaaaaaagt gcttattctg
     2341 ggagccggca ccatgggaca gcagatcggt tttttatgtg ccatgcacgg ctataacgtg
     2401 accatgtatg atatttcgga agacattctg aaagcctctg tcgcgcggat ggaaaaactc
     2461 ggttcatcat ggtttgtccg gatcaacagg attacgccgg agcagctcaa ggaaatcatg
     2521 agccgtatca cagcaacgtc tgatgcgaaa acagcggcca ggaatgccga cctgatcagt
     2581 gaatcggtgc cggaagatcc gaaactcaaa ggcaagattt ttgctcaatt caacacactc
     2641 tgcccgaaaa ggactatttt taccacgaac acctcgctgc ttatgccgtc aaaatttgct
     2701 gaagccaccg gcagaccgga aaaatttgcc gcgttgcatt ttcatgatgt gcgaacaacc
     2761 aatgtggtgg atgttatgcc tcatccggga acggataaag ccgtgaccan nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt gacgggacag atcaaaagtt gatatcggta
     2941 cgggagggtt gatatggatc cgtttcaaga cgttcatcat cccgatattg aatccgtcct
     3001 tcaggtttca cggtcaatat ttcattgact ctctttcttg ttttttttat aatcatcaca
     3061 gggccgtttt aagaggtcaa tacaacgagg aggaagcaaa atggcagaag acgatgatgt
     3121 tttgacgacg gatgataatg aaagtcctgt tagcggttac ctttccattg tattgacaat
     3181 cattggattg gtgatgcttt tgcttattaa aatactgggc attacgtcct ccgatgagac
     3241 cggctggtcc attgaatttg tattgtatct ttttatcagt atgacttttg ctattgccgg
     3301 cggattcctc ggttttctgg gattatcaga agacaacaat aaaaaaatat ttccgattat
     3361 cggtttgatt gtcgcggcaa tatacgtcgt ttcgctcggg tatgttatag ccacgtctat
     3421 ttcgtaaatg cttctgaaaa caataacgtt tctaatccgc tgctgcttga atttttattg
     3481 caggtcgcag cggattcttt tctgcagaca atcccctgat ctttcataaa tatcctgtgc
     3541 cggttatcga ggcaacaggc atgtttcccg gccggccata cggaggcgga aaatctgata
     3601 aacgggaatt gatcttaaag ccaaccgtcg cgagaccgtc tagcggtttg tgatttccac
     3661 caccaaagcg gtattgccct cggcatcggc tgccggaata tgcttctcct ccacccggcc
     3721 gatggtcgat aattgcctca acaagtcttt tatgttgtga cggttgagtt cacccgtcag
     3781 gaaggcttta tccggcatga ttgtcgcggt gatatttttt gcttcgcatt gtctaaatat
     3841 ttgttctgct tcccgcacgg ccacttcaag atccgtaacc ttcaatacaa tgccgggttt
     3901 tatatgtgaa tcttgcgccc gtggcgcgac gcttgccttc agggttgtcc ccgaaacgtg
     3961 acggtctttt gtctgtgcag attccacact gaattgttcc ggcacctgtg cgggagcaac
     4021 tttatttgaa tcatcccgcc cgtcggaagc cttcagttca ttgacatccg tttcatggac
     4081 atccgtcacc cggtgcactt cttttttacg gacagattcc cgtggtttgt ctttctggct
     4141 gatagtgttt ggttccggct gtgttcgttc agcgagggat gtgttttctt tggcttccgg
     4201 cgactgatct tgctgagtct gaagcaccgg cgccggtacg gatgaaggaa caacttcctg
     4261 aaaacggttt tcccccgccc ggtagaggta aacggcaagc acggcaatgc agaccgtggc
     4321 aaatatctga acgggaattt ttatctgcag gggatagaag aatttataaa gaaaactttt
     4381 ctttccggct tcctcgcgca cccgggccat gatttttttc cgaaaccagg gcggcggttc
     4441 gacctcatga aggctgtttg tcagttcttt cgttttgcgc agccgggcaa gtgcctcctc
     4501 gcattgccga cacgatcgca gatgttcgtc cacggctgct ttatcttctt tggaaagcac
     4561 atcgtccaga tacaaagaca ggttgttctc aatatccttg cattttttca taagtcacca
     4621 ataacctttt gcaggcaatc tttcagcgcg ttgcgggccc ttgaaagccg tgatttaacc
     4681 gttccatcag gcaccttgag gatggcgctt atttcttcat aagaaaagcc ctgaatgtct
     4741 ctcaatatca gcacgtcccg gtgttccgca tccagcgtgt tgatgcattt ctggacatac
     4801 gcttctcttt cgcgtttttc catctgtacg ccgggattac tctcctgact catcccctga
     4861 tcggagagtt gatcggcgtt tcttcccccg gtggcgtcaa tggaaaatcc ttcgcgccgg
     4921 cttcggccgc tcaactgctt taagcggttt ttggaataat tgacaacgat tcgatagagc
     4981 caggtggaaa attttgcttc cgccttgaac tgccggatgg atttgaaggc cgcgacaaag
     5041 gcttcctggg ttacatcaca ggcttcatcg taatcgccga tcatccggta agcgatgttg
     5101 agcattttct tctgatgcct ttccaccaga acgtcaaaag actctgtatc gcctttttga
     5161 caaagcgtga catatttcag atcttcatct ttggcggtct ttgccctggt gtttattata
     5221 ttagacatcc atagcaatgc ttttgttccc cttctatgtg gctcatacag agctcatttt
     5281 accgtgatct tgcgtgggaa gccggttgtg tgcattcgga tacgagaggc ggatcagtaa
     5341 aaaatcgctt tggattcgaa ttccctcaat tccctgaacg tgttttcaca gtcctgcttt
     5401 ctctgttcca gacgcgactg cctggcgaca atttccgccg gatgaagttg tttgccggcg
     5461 acattctggt tccagtcctg gatcatttga tgggcttccc ggcagacgtc attcattttt
     5521 ctccggagca gttcaaatcg tttaatctga gccggcgaat cgaattcaat gcccttgaag
     5581 taagaagcat attccttcat gcccggtttg atgacggttc cggatgtggt ttcaatggcg
     5641 actttttcca tcggaccgac ggtggccacc acccaggcgt gattgcttcc cattgccaac
     5701 tgccggaaat tccacgcggt gatactttcg tgcagggttc cgcccattat ctttgcttca
     5761 atgccgttgg tcaccagttg attccagaca tcgatagcca tgtccagaca aacgaattcg
     5821 ccctccaggg tatacgtgtg ccccttgtga aattccgcag caatttgttc caccattttc
     5881 attttgtccg gtttgggtga cgcctgccgg gttggcaggg gcggtatggc ctccggagga
     5941 ggtggcgcat cggacggtgt aaaaacctgc acgggtggaa gagctgcggg agaatccgct
     6001 gccgccggtg cggcgggaac aggtttggga gtcgcctgac ggggaggttt tgtaaaatga
     6061 taaaaaatca gtgcggccaa tatgccgatc gccatcagaa ttattacata atatttgcca
     6121 cggagaaccc gtgatgaatt attttttgga gccggtgcag acggtatttt attcccgcag
     6181 accgggcatt tgaaattacc cccgtgcagc gtatgcgcaa tgtcttcgga cttgaattcg
     6241 aaatggcaat gcgggcattc aatctgcatg atgcttttca tttccttgtt acgcgttatc
     6301 cgacaatgta agtgctggtg ccgacggcaa tcagctcacc gctgtcattg acaaattccg
     6361 tacgcgcgac agcaaccttg tgtcccagtc ttaaaagatg gccggtcgcc aggaagtatg
     6421 atcctttccc cggacgcata taatcgatcc gcatatcaat ggttcccatg ccggtcagac
     6481 gcttgacgat tttttccaaa ggcgcatcct tcatttcgtt aatgatgtgt atctgcgcga
     6541 tcacgctgcc ggtaaaatca agaatggtcg atatgacgcc gccgtgtaaa atgtttttct
     6601 caaaattgcc gatcagatca tcccgcatgt cgatccggac gaccgctttg tcggccttaa
     6661 tggaatcgat cttcagaccg aggtatttat taaacggcat cttttcttca tagacgattt
     6721 tcaactgctc aagaatgaca tctatcggca cagcatccat ggtcatggtt atcctgaaaa
     6781 ttttaatgta gagccgcctt ttatcacagc caggcttttt gtaaaacagt tttgttatcc
     6841 atcagcagtt tcatgtaatc gcgttcaagg cggatcatgt tcctgtcctt gcgacgcagc
     6901 cactcggcaa gccaggcgaa acgcagcgcg atgataaact cggtcagata tcgccagctg
     6961 atttgggaca tgatgcctga ccttttcatt ctgtcgataa aatgtccgac caggccgccg
     7021 gcaaggcttc gcgggttttc catgccgatg cagccgatga gattggcaac atcgtagagt
     7081 tcgcttttca acccgcagaa ttcccagtcg atgacgcatc ggataccgtc ggttgaccag
     7141 ataatattca gaggatgata gtcgccgtga caaaaggcca ccggcagttc ttcataaacg
     7201 ggcagaaaat ccttttccag aaaatcgacc acgctgccga tgtcgttttt gatgtctttg
     7261 ttgtaaagat tgatttcacg gaccaatttg taaatgtaat tcctcaggga gaacacgccg
     7321 cgggagccgg ccagtgtcat cttttgcgat ttgcgacgca gggcgatcag aaagtcagcc
     7381 agaacaatgc cgcgccacgg ctcaaaaaca tatttttcac ggtccagttc cacgccgggg
     7441 acaaaaggcg aaagttgcca gaaattattc tggtgttcga gaagataatt gcctctctca
     7501 tcggttagat agggattgat ttgagggagt ttcttcgcgg ccagcaaatc gagaattgcc
     7561 gcaatctgtt ttcttccagc cgccgattgc gatgcaattt gttccaggac gaagaatctt
     7621 ttgtctgtat cttccagaac cacacgaaac gcggaacgtt ccggacttcc ctgaacagga
     7681 attttacgat gtattttttt caggggaacc tgccacaggc cggccacggc tttcaatgtt
     7741 tctttgccga aaagggagtg ctctcctgcg gcaacaacgc gtctcttcga tgttgttgcc
     7801 gctgtggacg acatcctcgg cgcatgtttt ttcagggcgg acactttttt gtctttgggc
     7861 tgtcttttga tttttatttc cagtcgcatc gccgcgcttc tttccatcgc cgtaccggtg
     7921 accagtaatt ccaccggccg gcgggagcgg gtatatttgg cggctttgcc ctgatgatga
     7981 gcggccagac gtttttcaat gttattggtg atgcccgtat acaggcttcc gtcgcaacag
     8041 cgcagaatat aaacaaccca ggttttgctg tttttcttaa cgttgatact catgacctgt
     8101 attttctaca acagcgggaa ggacattgcc atagaaattt acaggcgtat ttccgaatat
     8161 tcgcttgttt atgaggctta aaagataatc cttgctattt atgaataata cctgtacaag
     8221 gcctcacagt gatcgcgata gttattgacc tcccttcctt tctacttcag aaatttcggc
     8281 aggaaaatcc ggctaatcgt aaccgaatcc aactttagcg ccaggtttgc
//
LOCUS       DAOOLB010000084         8297 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_10103_length_8297_cov_39.887891, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000084 DAOOLB010000000
VERSION     DAOOLB010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10103_length_8297_cov_39.887891"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="PLF77_09135"
     CDS             <1..789
                     /locus_tag="PLF77_09135"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPC09056.1"
                     /translation="GVRLDISGAVPENARTCLIVWPCMGGAVQMYRVPVEKFTDQGCA
                     CILYNPRGHGRSEGELEIQTALNDLDEILQENVPSAIPLVIVGHSAGANASLQFGVRC
                     LPPKQFVLIAPVLDSRESLFYMYQRKTIDEFVSILCAYVQDDTLVRKVLVDDQWLDAD
                     LWKARHYRHLLDRVHARMRIGTFLERLFIPGHNAFAQLERFSAQTRIFMAGEDTWYPA
                     ATTRRLADKYGIPVETLPEAQNHFFTGAWDSMWTKILNLILLPG"
     assembly_gap    1091..1190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1191..2841
                     /locus_tag="PLF77_09140"
     CDS             <1191..2841
                     /locus_tag="PLF77_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPC09057.1"
                     /translation="LSKIKHDTAEYRELSQKYSDATALFHARDGYAFESRAKQMLKGF
                     GFKESDFKQNCDSFSGGWKMRILLTSILLAKPDIMLLDEPTNHLDTESMEWLESYLKD
                     FRGTMIVISHDHFFLDKVTTQIAELSSCAIHLYKGNYTHYLTEKEKRQDALKKEMELQ
                     KSQIKKIEEFVERFRYKASKAAQVQSRIKMLEKFSEVTPEGKTKNVAMKFPEGKKSAK
                     EVVTVTGLGHRYGDLTVFGDLNFALFRGDKVAFVGMNGSGKSTLSRLLGKMENPSSGV
                     VKYGDGVNMAFYSQESSQNLNYEKTILEEINAVPTDASDQEKRNLLGTFLFSGDDIFK
                     PVSVLSGGEKSRLALLKIMLLDTNFLILDEPTNHLDIKTKDIFQNALIHYRGTVAIVS
                     HDRYFLDHLVNKVFELKDGAIHYYNGNYSYFIEQRSRPADAAGDEKPAQGVIRNQIVN
                     AKEKKRLEAEERNKTYKIRAALKKELSDVEKKISGLEDRKTRQETALCDPQTKKDART
                     IKSINLELKDINRDLEQSYTIWTDLHMKLEQISANAEPFSS"
     gene            complement(2838..3356)
                     /locus_tag="PLF77_09145"
     CDS             complement(2838..3356)
                     /locus_tag="PLF77_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPC09058.1"
                     /translation="MNKRISALKFALDNELHERDFYLANARRTKNLAGKNMFKQIADE
                     EKEHYDMLKKLFEKWEAKEKWPATIPLKVKKSVAGNIIKAMSEKKGARIKGNEDDLKA
                     IRTAIDFEARGMALYTKLEQGSDDPKEKAFFNLLASIERQHFLSLRDTEEFLTDPASW
                     YQNMEKASLDGG"
     gene            complement(3470..5074)
                     /locus_tag="PLF77_09150"
     CDS             complement(3470..5074)
                     /locus_tag="PLF77_09150"
                     /inference="COORDINATES: protein motif:HMM:NF037997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HPC09059.1"
                     /translation="MAIKDIFVIVSGVILFLIGMVNLSSSIRKLIDVRIKKFIKYAVG
                     RPISGLLTGIVSTIAFQSSSASTVLTIGLVSAGLISFSNSLAIILGADIGTTLTVQLV
                     IWRFTDISPLIISLGGLLWLIGRNKWETIGEIIFYFGLMFFGLDLVNKAVEPLKQSPL
                     LIELFTKTQNPLIGIGMGIAVTGIVQASAIPISIMVLLAQQDIVSLENAVAVVMGANI
                     GTTITALLAGTVATRSGKRTAFSHLIFKSTGIGLCFMAMPSFVWILQHISSSVAQQIA
                     LAHFLLNLFIVVIFIFLLSPFAAMMTRFFPGKDDILPVWPEYLDVQDITNPQKALDNV
                     HKELKRQTNMVRDMYLKSMRMINDYSPSDDKSLFYIAMVVKNIREQIVGYLWKLSVQD
                     LSEYYSKNVFAYTAMTSDIESIGNHVLCISKLTAQKTGKNIKFSDCGEVELHEIIDLV
                     SDNFNDAFSVIDKPLDKTIMDVIAREEQVDIKVKEARDNHLERFHKRLCDPEAGPIFV
                     ELLLHLERISDLCNNIAEYMLDVKED"
     gene            complement(5411..6433)
                     /locus_tag="PLF77_09155"
     CDS             complement(5411..6433)
                     /locus_tag="PLF77_09155"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09060.1"
                     /translation="MNKLCYDIPVLLLHNMNPEWTSEETLETEQDVATLGNALKDLGH
                     PLEIVTVRDDRLAQQLHNFNPRDYVVFNWCEELPGVPHSDPDVAAVMERMGFVFTGSS
                     RDVLALAHDKPEIKRVLDDANIPTPPWAVFDNTDVGEWNIFPSIVKAANEHCSIGISP
                     ESVVTSPKELENRVAFILDKYQQPAIVEQFINGREFLNAVWGNGHISMLPAVEMDYSE
                     LTDLRDRLFSYEAKYVSGSRLYESIDMRVPAELETSAMAQLENVVIATYRATGCRDYG
                     RIDVRYLNGIFYVIDVNPNPDINPQTSMTHAAAEIGYSYGQMGSRIVNLAAARHPIFS
                     QQYNPV"
     gene            6741..7157
                     /gene="rnk"
                     /locus_tag="PLF77_09160"
     CDS             6741..7157
                     /gene="rnk"
                     /locus_tag="PLF77_09160"
                     /inference="COORDINATES: protein motif:HMM:NF004396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HPC09061.1"
                     /translation="MADRTIYITKFDMDRLLELIDGVRSTPRHNKANIDLLEKELYNG
                     TVVDSEDVPADVITMNSRVSVTDIESGEQMTYTLVFPSEANIAENKLSILVPLGMALI
                     GYRKGDIIEWPVPSGVRKIKVNDVIYQPEAAGNYNL"
     gene            complement(7209..>8297)
                     /gene="rfbB"
                     /locus_tag="PLF77_09165"
     CDS             complement(7209..>8297)
                     /gene="rfbB"
                     /locus_tag="PLF77_09165"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HPC09062.1"
                     /translation="IRMTKNIHPPVTLHNLLVTGGCGFIGSNFIRYLIDRIHHSGRII
                     NVDKLTYAGNPENLAEIAANGASRYIFQKADICDRARLEEIFSRYDIDGVCHFAAESH
                     VDRSIKTPGDFIQTNIVGTFNLLECAKARGDRFKIFHHVSTDEVYGSLGADGLFREDT
                     PYRPNSPYSASKAASDHLVRAYYKTFGLPVTISNCSNNYGPYQFPEKLIPLIVLNALE
                     GKPLPVYGDGKNVRDWLYVEDHCEAIWTIMTAGRHGETYNVGGKAEMENIAIVQLICD
                     ILDDIQPASDNSPRRRLITFVKDRPGHDRRYAIDFSKLQKELNWAPKESFSSGLQKTV
                     NWYLDQKKWVENIKTGAYLNWIKEQYGA"
BASE COUNT         2189 a         1852 c         1994 g         2162 t
ORIGIN      
        1 ggcgttcgcc tggacatatc aggcgccgta ccggagaatg ccagaacctg cctgatcgta
       61 tggccctgca tgggcggtgc agtgcagatg tatcgcgtac cggtggaaaa atttacagat
      121 cagggatgcg cctgtattct ttataatccg cgcggccacg gtcgttccga gggggaactg
      181 gaaattcaaa cggctttgaa tgacctggat gagattcttc aggagaatgt tccttccgca
      241 attccgctgg tgatcgtcgg ccacagtgcc ggagcgaacg ccagtctgca attcggagtc
      301 cgctgtcttc cgccgaagca attcgtgctg attgcgccgg ttttggattc acgtgaatcc
      361 ctgttttaca tgtatcaaag aaaaaccatt gatgaattcg tcagtattct ttgcgcctat
      421 gtacaggatg acactctggt aaggaaagtg cttgtcgatg atcaatggct ggatgccgat
      481 ctatggaaag cccgtcatta cagacatctg ttggatcgtg ttcatgcgcg tatgcgcata
      541 ggcacattcc tggaaagact ctttatcccc ggacacaatg cctttgctca actggaaagg
      601 ttttccgcgc agacgcgcat ctttatggcc ggggaggaca cctggtatcc ggccgcgacg
      661 acccgtcgtt tagcggacaa gtacggtatc cctgttgaaa cactgccgga agcgcaaaac
      721 cattttttta ccggtgcctg ggacagtatg tggacaaaaa ttcttaatct gatcctttta
      781 cccgggtagt tttctttctt ttcagcgatg tttccctgat acaaatcata aggggcatca
      841 aacaagccgg cgtcctgcaa acgattttaa gcgcaaccgc tcacttgatt cgtcaacgtt
      901 tgtggtcatt tctatgaatg tcgttccttc aggaaacagt ctgcgcgaag gattactctt
      961 gtagtttttt gataaatttg tcatataacc tccacttacg gagggtatat ttattgattt
     1021 atttccgcaa catcagtctt tcgtttctgg ataaaaaact gttcgacaag atgaactgga
     1081 ccatccaccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gttatccaaa
     1201 atcaagcatg acacagccga atatcgcgag ctttcgcaaa aatattccga tgcgacagca
     1261 ttattccatg ccagggacgg ttacgctttt gaatccagag ccaagcagat gctcaagggg
     1321 ttcggcttta aagaaagtga ttttaaacaa aattgcgatt cgttttccgg tggctggaag
     1381 atgcgcattt tgttgacatc gattcttctg gcaaaaccgg atatcatgct gttggacgaa
     1441 ccgacgaacc atctggatac ggaaagcatg gagtggttgg aatcgtatct caaggatttc
     1501 cgggggacca tgattgtcat ttctcacgat catttctttt tggataaagt cacgacgcag
     1561 atcgcggagt tgtcctcctg cgctattcac ctttacaagg ggaattacac gcactatctg
     1621 acggaaaaag aaaagcggca ggacgctttg aaaaaggaaa tggaactgca aaaatcgcaa
     1681 atcaaaaaaa tagaagagtt tgtggagcgg ttccgctaca aggcgtcaaa agcggcccag
     1741 gtgcaaagcc ggatcaaaat gctggaaaag ttttccgagg taaccccgga gggaaaaaca
     1801 aagaatgtgg ccatgaaatt ccctgaaggg aagaaaagcg ccaaagaagt cgtcacggta
     1861 accggcctgg gacaccgcta cggcgatttg acggtgtttg gcgatttgaa ctttgcgcta
     1921 ttccgcggcg ataaagtggc ctttgtcggc atgaacggct caggaaaatc aaccttatcc
     1981 agattacttg gcaaaatgga gaacccttcc agcggcgtgg ttaaatacgg cgacggtgtg
     2041 aacatggctt tttattctca ggaaagttcc cagaacctga attacgaaaa aaccattctg
     2101 gaagaaatca atgccgttcc gaccgatgcg tcggaccagg aaaaacgcaa tctgctgggc
     2161 acttttttat tttccggaga tgatatcttc aaaccggtat ccgtgctttc cggcggggaa
     2221 aagtcaagac ttgcccttct aaaaatcatg ttgttggata ccaatttcct gattcttgac
     2281 gaaccgacca accatctgga tatcaaaaca aaggacattt ttcaaaacgc tctgatccat
     2341 taccgcggga ccgtggcgat cgtgtcgcat gaccggtatt tcctggatca tctggtcaat
     2401 aaggtgtttg aactgaagga cggagcaatc cattactaca acggcaatta ttcttatttt
     2461 atcgagcagc gcagccggcc ggcggatgcc gcaggcgatg agaagcctgc gcaaggcgtg
     2521 atcagaaatc agatcgtaaa cgccaaagag aaaaaacgtc tggaagcgga agaaagaaac
     2581 aaaacgtata agatcagagc ggcgctcaaa aaggaactgt ccgatgtgga gaaaaaaata
     2641 tccggactgg aagaccggaa aaccagacag gaaacggcat tatgtgatcc gcaaactaag
     2701 aaagatgccc ggacaataaa atcgatcaac ctggaattaa aagatatcaa tcgcgacctg
     2761 gaacaatcct atacgatctg gacggatttg catatgaagc tggaacaaat atccgcgaac
     2821 gctgagcctt tttcctctta accgccgtca agagatgctt tctccatatt ctgataccag
     2881 gacgccggat cggtcagaaa ttcctctgtg tcacgtaacg aaagaaagtg ctgtctttcg
     2941 atgctggcga gcagattgaa aaaagctttt tctttgggat catcgcttcc ctgctccagt
     3001 ttggtgtaga gagccatgcc tctggcctca aagtcaatag cagtccgtat ggctttcagg
     3061 tcatcttcgt tgcctttgat acgcgcccct tttttctccg acatcgcttt gataatatta
     3121 ccggcgacag attttttgac tttcagaggg atggtcgccg gccatttttc cttcgcctcc
     3181 catttttcga agagtttttt cagcatatca tagtgttctt tttcttcgtc ggcaatctgt
     3241 ttaaacatgt ttttcccggc cagatttttt gttcgacggg cgttagccag ataaaaatct
     3301 ctttcatgta actcattgtc cagggcgaat tttaaagcgc tgattctctt attcatcttg
     3361 atacctcctc gagcggatgt ccaagtaaac agtccctcca cgacatatta tatgagatat
     3421 tgcgcatgag ggcaataggt ttgtttttta aaggtcgggt actcgggatc tagtcctctt
     3481 tcacgtcaag catatattcg gcgatgttgt tgcaaagatc agaaatgcgt tcgaggtgca
     3541 aaagcagttc aacaaaaatg ggcccggctt ccggatcgca aaggcgtttg tggaatctct
     3601 ccaaatgatt gtcccgggct tctttgactt tgatatccac ctgttcctca cgggcaatga
     3661 cgtccatgat ggttttatcc agcggcttat cgataacgga aaaagcgtcg ttaaaattat
     3721 cactgaccag gtcgatgatt tcatgaagtt cgacttctcc gcagtcgctg aatttgatat
     3781 ttttaccggt tttttgagcg gtaagcttgc tgatgcataa aacgtgattg ccgatacttt
     3841 caatatcact ggtcatggcg gtatatgcaa aaacgttttt agaataatat tcagaaagat
     3901 cctgtacgga taatttccag agataaccga cgatttgttc ccggatattc ttcaccacca
     3961 tggcgatata aaaaagggat ttatcgtccg acggactgta atcgttaatc attctcatgc
     4021 tctttaaata catgtcgcga accatgttgg tttgacgctt cagttctttg tggacgttat
     4081 ccagtgcttt ctgcggattg gtaatgtcct gaacgtcgag atattccggc cagacaggga
     4141 gaatgtcgtc cttgccgggg aagaaccggg tcatcatggc ggcaaacggg gacagcagaa
     4201 aaatgaaaat cacgacaata aacaagttta acagaaaatg ggcaagggcg atctgctgcg
     4261 cgacgcttga cgaaatatgt tgcaggatcc agacaaaaga gggcattgcc atgaaacaca
     4321 atccgatccc ggttgattta aaaatcaagt gagaaaaagc cgttcgtttt ccgctgcgcg
     4381 tagcgaccgt tccggccagc agggcggtta tcgtagttcc gatgttggct cccatgacca
     4441 cagccacggc attttccagc gagacaatat cctgctgagc caaaagaacc atgatactga
     4501 tcgggatggc cgatgcctgg acaatgccgg tgacggcaat tcccataccg atgccgataa
     4561 gcggattctg tgttttcgta aacagctcaa tgagcagagg ggattgcttg agcggttcca
     4621 cggccttgtt gaccagatcc agaccaaaga acatcagacc gaaataaaag atgatctctc
     4681 cgatcgtttc ccatttattc ctgccgatca gccacaataa tccgcccaag gaaatgatca
     4741 gcggcgatat atccgtgaag cgccagatga ccagttggac ggtcaatgtt gtaccgatat
     4801 ccgcgcccag aataatggcc agagaattgg agaagctgat taacccggcg ctgaccagtc
     4861 cgatggtcag gacagtcgat gcggaggagc tttgaaaagc aattgttgaa acgatgcccg
     4921 tcagcaatcc ggaaatcggc ctgccaacgg catattttat gaatttttta atgcggacat
     4981 cgatcaattt ccttatggag gaagaaagat tgaccatgcc gatcagaaat aagatgacgc
     5041 cactgacgat gacaaaaatg tcttttattg ccaaatttta ctcctggtta agtttaaatt
     5101 tccgtttggc gccgggttgt cattttttta atctatccgt caaacgattt gaacagtttc
     5161 tcttttgcac agcatcgtta acaaaatgaa aagatcgtgt tgcatctttt gcgtttttga
     5221 caagatcgtt ttcttgaata acgttattgt gttgatgaat gaatattgac aaatttatct
     5281 taaaaagaca agggcaatga tgaatatata agatcgttca gaaagaacgg atcatgtttt
     5341 ttattcggat cggataagat gcgttatcat tctgatgcga cgcgatgctt cccgatgagg
     5401 aaaatctgtt ttatacggga ttgtactgtt gactgaaaat tggatggcgt gccgctgcca
     5461 gattgacgat gcggcttccc atttgtccgt aggagtaacc gatttctgct gcagcatggg
     5521 tcatcgacgt ttgcggattg atatcgggat tcgggttgac atcaataacg taaaaaattc
     5581 cgtttaaata acgaacgtca atgcgtccgt aatcgcgaca ccctgtggcc cggtaggtgg
     5641 caatcaccac attttcgagt tgggccatcg ccgacgtctc cagttccgcc ggtacgcgca
     5701 tatcgatgct ttcataaaga cgcgacccgg aaacgtattt ggcctcatag gaaaaaagac
     5761 ggtcccgcag gtccgtcagc tcgctgtaat ccatttccac agcgggtaac atggaaatgt
     5821 gtccattccc ccagacggca ttgagaaatt cgcggccgtt aataaattgt tcaacaatag
     5881 ccggttgttg atatttatcc agaataaacg ccacacgatt ttccagttct ttcggagacg
     5941 tgacgaccga ttccggagag ataccgatgc tgcaatgttc attggccgct ttgacaatgg
     6001 acggaaaaat attccattcc ccgacatcgg tattatcaaa aaccgcccat ggcggagtcg
     6061 gaatattggc gtcgtccagg actcgcttga tttccggctt gtcatgcgcc agtgccagca
     6121 cgtcgcggga ggaaccggtg aagacaaagc ccatacgttc catgaccgcg gcaacatcgg
     6181 gatcgctgtg cggcacgccg ggaagttcct cgcaccagtt gaacacgaca taatcccgcg
     6241 gattgaagtt atggagctgt tgggccagac gatcgtcccg caccgtgacg atttcgaggg
     6301 gatgtcccag atccttcagg gcattgccga gtgttgccac gtcctgttcg gtttcaagag
     6361 tttcttcaga tgtccattcg ggattcatgt tgtgcagcag aagaaccggt atgtcgtaac
     6421 agagtttgtt cataacacgc ctgtctgaac gtccggctaa acgacttttg cctgttgatg
     6481 ccattttacc gggcgaaatc gtttttgtga tgcaaaatgt ttacgaccgt ttttactata
     6541 acagatttta tccaaacagt tcaaggaaaa atgaatctta tccgttcaga tattttaaac
     6601 ggtggtgaaa tatgcttaag ggattgacag ccggctttta tttgcatata gtaattatac
     6661 aacggatttg aaaaagcatt taaccttatt tttatccaaa cataataacg aagcaatgaa
     6721 cttcagggag atggatcatc atggcagatc gcacgatata cattacaaaa ttcgatatgg
     6781 atcgtttact ggaattaatt gacggcgtgc ggtcaacgcc caggcacaac aaagccaata
     6841 tcgacctctt ggaaaaggaa ttatacaacg gtacagtggt tgattctgag gatgtgccgg
     6901 cggatgtgat cacgatgaat tctcgggtca gcgttacgga cattgaatcc ggagaacaaa
     6961 tgacctatac ccttgttttt ccatccgaag ccaatattgc ggaaaacaaa ctgtccattc
     7021 tggttccgct gggaatggct ctcatagggt atcgtaaagg ggatatcatc gaatggcctg
     7081 tgccgtccgg cgtcaggaaa ataaaggtca atgatgttat ttatcagccg gaggctgcgg
     7141 ggaattataa tttataaaca gagaaccttc ttgccccggg gcaaagggtg attcttttct
     7201 cgagaaaact acgcaccata ctgctctttg atccaattca ggtaagcacc ggttttgatg
     7261 ttttcaaccc atttcttttg gtcgaggtac cagttaacgg ttttttgcag accggaggaa
     7321 aacgattctt tcggcgccca gttcagttcc ttttgcagct tagaaaaatc aatggcataa
     7381 cggcggtcgt gtccggggcg atccttcaca aaggtaatca accggcgcct cgggctattg
     7441 tcggacgccg gttgtatgtc atccagaata tcgcagatca actgcacaat ggcaatattt
     7501 tccatttccg ccttgccgcc gacattgtac gtttcaccat gccggccggc tgtcatgatg
     7561 gtccagatgg cctcacagtg gtcctcgaca taaagccagt cgcggacatt tttaccgtca
     7621 ccgtaaacgg gaagcggttt gccttccagg gcgttgagaa cgatcagggg gatgagcttt
     7681 tccggaaact gatagggacc gtaattattg gaacagttgg aaatggttac aggcaatccg
     7741 aacgtcttat agtaagcccg caccagatga tcggaagcgg ctttggatgc ggaatagggg
     7801 ctgttcggac gataaggcgt gtcttcacgg aaaaggccgt ccgctccaag actgccgtag
     7861 acctcgtcgg tactgacgtg gtgaaaaatc ttgaaccggt cgccgcgcgc tttggcgcac
     7921 tccagaagat tgaaggttcc gacgatgttg gtctgaatga aatctccggg cgttttgatg
     7981 gaacgatcca catgcgattc ggctgcaaag tggcaaacgc cgtcaatatc gtatcgggaa
     8041 aagatttctt ccagacgggc cctgtcgcat atatccgctt tttgaaaaat gtatcgtgat
     8101 gcaccgtttg cggcaatctc tgcaagattc tcaggattcc ccgcgtaagt taatttgtcg
     8161 acattgataa ttcttcctga atgatgaatc cggtcgatta aataccggat gaaattgctg
     8221 ccgataaaac cgcagcctcc cgtaaccagt aagttatgca atgtgacagg gggatggatg
     8281 ttttttgtca ttcggat
//
LOCUS       DAOOLB010000085         7985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_10627_length_7985_cov_44.625095, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000085 DAOOLB010000000
VERSION     DAOOLB010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10627_length_7985_cov_44.625095"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..1009)
                     /gene="dapA"
                     /locus_tag="PLF77_09170"
     CDS             complement(128..1009)
                     /gene="dapA"
                     /locus_tag="PLF77_09170"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041584832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPC09063.1"
                     /translation="MFRGAMVAIVTPFKNDRIDEEALRDLIEFQIASGTDAIVPCGTT
                     GESATLTHEEHDRVIEITIDAVKKRVPVIAGTGSNNTAEALRLTKHAWEAGADGALIV
                     APYYNRPTQEGLYQHFKIIAESVPIPIIPYNIPGRTGVNMLPGMVAKLAQIKNIVGMK
                     EASGSIKQMSDVIDLCGKDFAVLSGDDIFTLPLMAIGGAGVISVISNVAPADMAGLID
                     AFHAGDMARAKSLHYKMAPLIEALFLEVNPIPVKAALALMGKINGEYRLPLCRMSEGN
                     FETLEKVMTDYGLIPPS"
     gene            complement(1033..>1501)
                     /locus_tag="PLF77_09175"
     CDS             complement(1033..>1501)
                     /locus_tag="PLF77_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09064.1"
                     /translation="RGLKNLKSVSRRVKIFLAVGMLAILAACGVKGNPAGPPTGETHS
                     RAVGNLTVTPSGDHVVLTWALQDEHSRIRSIGIERIEYAAEDAECRNCADKFERIGRL
                     LAGTTGQNGPARKSFEFVDKNVTQGIRYGYRLSLCDEFDECFNSEVTEVYVSR"
     assembly_gap    1502..1601
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1602..2635)
                     /gene="argH"
                     /locus_tag="PLF77_09180"
     CDS             complement(<1602..2635)
                     /gene="argH"
                     /locus_tag="PLF77_09180"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HPC09065.1"
                     /translation="MSAGKKPWSGRFQKSTARSVEKFTASIHYDHRLYPCDIEGSIAH
                     ATMLAKQKIIAKKDAQKIIAALKAILKDLEQGKLRLDPADEDIHMAIEKELIRRIGEA
                     GGKLHAARSRNDQILLDERLYLRAEIQTIAGRLTSLQRALVQLAKREIKTIMPGYTHM
                     QKAQPVLLSHYLLAFADMFGRDVRRLQDCRERTNVLPLGAAALAGTGLPIDRAETARL
                     LNFPGISTNSMDTVADRDFIAEFIFDAALIMMHLSRFCEDLVLWSSEEFGFIEISDAY
                     TTGSSIMPQKKNPDVAELIRGKTGRVYGDLFAILTLLKGLPMTYNRDLQEDKEPLFDA
                     VDTVKSCLSI"
     gene            complement(2653..3867)
                     /locus_tag="PLF77_09185"
     CDS             complement(2653..3867)
                     /locus_tag="PLF77_09185"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HPC09066.1"
                     /translation="MAAKKRKVVLAYSGGLDTSVIVRWLIETYKCEVICFAADVGQKE
                     ELTGLKEKAIKTGASKIYIDDLREEFARDFVFPALRANAIYEGTYLLGTSLARPLIAK
                     RQIEIAKLEGADTVSHGATGKGNDQVRFELTYYALNPNIKIIAVWKDDNWTFRSREEM
                     IDYAGKYKIPIPLSKAKPYSADRNLLHISHEGGILEDPWAEPPEDIFVLTRSPENAPD
                     KPTYVEIDFKKGNPAAVNGKKMSPAKLMDYMNTVAGINGIGRVDMVENRFVGMKSRGV
                     YETPGGTVLWSAHRALESITMDREVMLMRDGLTPKYAQLAYNGFWYAPEMKALQTFID
                     ATQENVNGTVRMKLYKGNAIVAGRKSPNSLYSESFATFEKDQVYNQKDAIGFIKLNGL
                     RLIIENILKNKP"
     gene            complement(3920..4828)
                     /gene="argF"
                     /locus_tag="PLF77_09190"
     CDS             complement(3920..4828)
                     /gene="argF"
                     /locus_tag="PLF77_09190"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPC09067.1"
                     /translation="MKKDLLSLYDLDTKDFDIIWRKAGKLKKYLKAGKVHDSLKGKTL
                     GMIFDKSSTRTRISFEVGMYQLGGIALFLTSRDTQIGRGESVADSARMMSRYLNGIMI
                     RTFSQESVEELAQWATIPVINGLTDLLHPCQILSDLFTIIEKKGSYRNVKIAYIGDGN
                     NVANTWIEAAARLPIRLSLACPKGYEPDRTIMTKGRRESQEGIVLCHDPFEAAKNADV
                     LYTDVWVSMGQEAEDKKRKKIFKDYQINSNILKAAKKDAIVMHCLPAHRGEEISSDVI
                     DGPHSVVIDQAENRLHVQKAVLELLL"
     gene            complement(4825..6015)
                     /locus_tag="PLF77_09195"
     CDS             complement(4825..6015)
                     /locus_tag="PLF77_09195"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HPC09068.1"
                     /translation="MNEQEVIAQATGNIMNTYRRFPIVLTKGSGARVWDINGKEYLDL
                     VAGIAVCNLGHSHPQVIAAVKEQLDKLTHVSNLYYTQPQTELAELLIRHSFADKVFFC
                     NSGAEANEAAIKLARKYAHENMGPDKFELITMKDSFHGRTMATITATGQEKFQFGFTP
                     LLDGFTYVPFNDLSALDKAVSSRTCGVLLEPIQGEGGVNIPDDDYLARVREICDRHGI
                     LMILDEVQTGIGRTGKLFAYEYSAIKPDIMTLAKALGNGFPVGAMLATDKVAKAFVPG
                     NHASTFGGNPLAMAAGTAVLKTILQENLLEHCRKTGAYFLSQLKKLQQKHAVINEVRG
                     KGLMLAVKLNIDAAGIVGECLKQGLLVNSTGGQVLRFVPPLIITEQDIDQTVAVLDSV
                     LEGK"
     gene            complement(6039..6929)
                     /gene="argB"
                     /locus_tag="PLF77_09200"
     CDS             complement(6039..6929)
                     /gene="argB"
                     /locus_tag="PLF77_09200"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HPC09069.1"
                     /translation="MENISNSIERADILLETLPYIRRFYNKTIVIKYGGHAMVDKDLK
                     DRFAQDIVMMKYIGINPVVVHGGGPQIGELLKKVGKESKFIQGMRVTDEETMNIVEMV
                     LVGMVNKEIVGLINRHGGNAVGLSGKDGNLITAEKYYLNEEKAKHTPPEIIDIGLVGK
                     VKSINTQLITSLAHGNFIPIIAPTGLGDNGETYNINADIVAGEIAAALKAEKLLLLTD
                     VPGVLNKKKELIHTMTNDEALNLIEDGTIEGGMFPKIKCCLKALNGGVQKAHIVDGRL
                     KHAVLLEVFTDKGIGTEIVL"
     gene            complement(6945..>7985)
                     /gene="hslU"
                     /locus_tag="PLF77_09205"
     CDS             complement(6945..>7985)
                     /gene="hslU"
                     /locus_tag="PLF77_09205"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HPC09070.1"
                     /translation="QESVQAKAAEIAEERVLDLLLPPRLTEKKVDDILSDQPQEQTVL
                     TMEAEQKKNDATREKMRIWLKEGKFDDRMVELEVTEARSGPMIEIFSAAGMEDMGLNL
                     KDMLGNMMPQKKKRRTVKVPEALEILASEETQKLIDMEKVTRTALERVEQSGIIFLDE
                     IDKIVGGDSQHGPDVSREGVQRDLLPIVEGSNVNTRYGMVKTDHILFIAAGAFSGTKP
                     SDLIPELQGRFPIRVELDSLDKEDFIRILTEPNNALVKQYREMMATEGLRLVFKEDAI
                     AAIAEMATVVNENTENIGARRLYTMMEKLLDDISFDAPDLDKKDIVIDAQYVEEKLND
                     IIEDQDLSRYIL"
BASE COUNT         1639 a         2326 c         1876 g         2044 t
ORIGIN      
        1 tctacggcgc cgaccaccct gatgttgtcc atggtcgaaa tcagactgat gattttgccg
       61 cccattcttc cggcagcccc ggttaccacg gcttttacca taggttgatc tccttttctt
      121 tatgacatca cgacggcgga atcaatccgt agtccgtcat gaccttctct aacgtttcaa
      181 aattgccttc ggacattcta cacagcggca atcggtattc gccattgatt ttacccatca
      241 gggcaagcgc cgccttgacc ggaatcgggt tgacttcgag aaacagggct tcgatcaaag
      301 gcgccatttt gtaatgcagc gattttgccc gggccatatc tcccgcatgg aaggcatcga
      361 tcaggccggc catgtccgcc ggcgccacat tcgaaatcac ggaaatcaca ccggccccgc
      421 cgatggccat caacggcagc gtgaatatat catccccgga taaaacggca aaatcctttc
      481 cgcacagatc aatcacatcg ctcatctgtt tgatggagcc agaggcctcc ttcattccga
      541 cgatattctt gatctgcgcc agtttcgcca ccatcccggg cagcatattt acaccggtgc
      601 gtcccggtat gttgtaggga atgatgggaa tgggcacact ctcggcgatg attttgaaat
      661 gctgataaag cccctcctgc gtcggacgat tataataggg ggcgacgatc agcgcgccgt
      721 cggctcccgc ctcccaggca tgcttggtca atcgcagggc ctcggccgtg ttgttggagc
      781 cggttccggc gatgaccggc acccgctttt tgacggcgtc gatcgtaatt tcgatgacgc
      841 gatcatgctc ttcatgggtc agcgtggccg attcgccggt tgtcccgcag gggacgatcg
      901 cgtcggtgcc gctggcaatc tgaaattcga tgaggtcgcg cagagcctcc tcgtcgattc
      961 ggtcgttttt aaacggggtg acaattgcaa ccattgcgcc tctaaacata aaagaagcct
     1021 ccttctctca attcatcgcg aaacatatac ttccgttacc tcactgttaa aacattcgtc
     1081 aaattcgtca cacagtgaca aacgatagcc gtagcgtatg ccttgcgtga catttttatc
     1141 aacaaactca aaactctttc gggccggacc gttctgtccg gttgtcccgg ccaacaaacg
     1201 tccgatgcgt tcaaatttat ccgcgcaatt ccggcattcg gcatcttcgg ctgcgtattc
     1261 gatgcgttct atgccgatgg aacggattcg tgaatgctca tcctgcaacg cccacgtcag
     1321 caccacatga tcccccgagg gggtaacggt taaattgccg accgcccggg aatgcgtctc
     1381 gcccgtgggc ggaccggcgg gatttccttt gacgccgcag gccgccagta tcgccagcat
     1441 ccctaccgcc agaaatattt taactcttcg agaaacgctt ttcaaattct ttaatcctct
     1501 gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naaaatcgaa aggcagcttt
     1621 tcaccgtatc caccgcatcg aaaagcggct ccttgtcttc ctgcaaatcc cggttgtatg
     1681 tcatgggcag cccttttaaa agcgtcagaa tcgcgaacag atcgccgtat acccgccctg
     1741 tttttccccg gatcagctcc gcgacatccg gatttttttt ctgcggcatg atgctgctgc
     1801 cggtcgtata ggcatccgat atttcaataa aaccgaactc ctcggacgac cacagaacca
     1861 gatcttcgca aaaccggctt aaatgcatca tgatcagggc ggcatcgaaa ataaactccg
     1921 cgataaaatc ccggtccgcc accgtatcca tgctgtttgt gctgattccg ggaaagttca
     1981 gcagcctggc ggtttccgcg cggtcaatcg gcagccccgt tccggccagc gccgccgcgc
     2041 ccagcggcag aacattcgtc ctttcgcggc aatcctgaag ccgccgcaca tcgcgtccga
     2101 acatatccgc aaacgccaga agataatgcg acagcagcac cggctgcgcc ttctgcatat
     2161 gcgtatagcc gggcatgatc gttttgattt cccgtttggc cagttggacc agggcgcgct
     2221 gtaacgatgt cagacgtcct gcgatggtct gaatttccgc ccgaaggtaa agcctttcat
     2281 ccagcagaat ctgatcgttg cggcttctgg ccgcatgcag tttgcctccc gcctctccga
     2341 tgcgccggat cagttccttt tcgatggcca tgtggatgtc ttcatcggcg ggatccagcc
     2401 ggagttttcc ctgctcgagg tctttcagaa tcgccttcaa cgcggcgatg attttctgcg
     2461 catccttttt ggcgataatc ttctgtttcg ccagcatcgt cgcatgggca atcgatccct
     2521 caatgtcgca gggatagagc ctatgatcgt aatgaatgga cgctgtaaat ttttcgacgc
     2581 tcctggctgt ggatttctga aaccttccgc tccatggctt tttcccggca gacatttttt
     2641 ctccccgatc cattaaggtt tatttttcaa aatattttcg ataatcagcc gcaatccgtt
     2701 gagcttgatg aaaccgatgg catccttctg gttgtacacc tgatcctttt caaaggtggc
     2761 gaacgattcg ctgtacaggg aattcggcga tttgcggccc gccacgatcg cgttgccctt
     2821 gtataatttc atccgcaccg tgccgttgac gttttcctgc gtggcatcaa tgaaggtttg
     2881 cagcgccttc atttccggag cataccagaa cccgttgtag gccagctgcg catatttcgg
     2941 cgtcaggccg tcgcgcatca gcatgacttc acggtccatc gtgatggact ccagcgcgcg
     3001 gtgcgccgac cagagcacgg ttccgccggg ggtctcgtaa acgccccgcg atttcatgcc
     3061 gacaaaacgg ttctccacca tatcgacacg gccgatgccg tttatcccgg caaccgtatt
     3121 catgtaatcc atcagttttg ccggcgacat ctttttgccg ttgacggccg ccggatttcc
     3181 cttcttgaaa tcgatttcca cgtacgtcgg tttgtccggc gcattttcgg gcgagcgggt
     3241 caatacaaag atatcctccg gcggttcggc ccacggatct tccagaattc cgccttcatg
     3301 cgaaatatgc agaagatttc tgtccgcact gtagggtttg gccttgctca acggaatggg
     3361 aatcttgtat ttcccggcat aatcgatcat ctcttcccgg gaccggaagg tccagttgtc
     3421 gtccttccag accgcgatga ttttgatatt cggattgagc gcataatagg tcagttcaaa
     3481 gcggacctga tcattgccct tcccggttgc gccgtgggaa accgtatcgg cgccctccag
     3541 cttcgcaatt tcgatctgcc gtttggcgat cagcggccgc gccagcgatg tgcccagaag
     3601 ataggtgcct tcataaatcg cgttggcccg aagcgcgggg aaaacaaaat cgcgcgcgaa
     3661 ctcctcccgc agatcgtcaa tgtaaatttt gctcgctccg gttttgatcg ccttttcttt
     3721 caggccggtc aattcctcct tctgtccgac gtctgcggca aagcagatga cctcgcattt
     3781 ataggtttca atcaaccagc ggacaatgac cgatgtgtcc agtcctccgg aatacgccaa
     3841 aacaactttt cttttctttg ctgccaatgt ggatgtcctc ctgaccccga tttgcgggat
     3901 gaatatgttg atcaaatcgt tacagtaaca attccagaac ggccttttgc acatgcagac
     3961 ggttttccgc ctgatcgatg accaccgaat gggggccgtc gatgacatcg gaggaaatct
     4021 cctcgccccg gtgcgccggc agacaatgca tcacgatggc atcttttttg gccgccttca
     4081 aaatgttgct gttgatctga taatctttaa atattttttt ccttttttta tcctccgctt
     4141 cctgccccat gctgacccag acatcggtat agagcacatc cgcgtttttc gccgcttcaa
     4201 acggatcgtg gcacagcaca atgccttcct gcgactcgcg gcggcccttc gtcatgatcg
     4261 ttcggtccgg ctcatagcct ttcggacagg ccagcgacag acggatcggc agccgggcgg
     4321 ccgcttcgat ccaggtattg gccacgttat tcccgtcgcc gatgtaggcg atcttgacgt
     4381 tacgatagga ccccttcttc tcgataatgg taaacagatc gcttagaatc tgacagggat
     4441 gcagaagatc cgtcagaccg ttgattaccg gaatcgtcgc ccactgcgcc agttcctcca
     4501 cgctttcctg cgaaaatgtc cggatcataa tgccgttgag atagcgcgac atcatccgcg
     4561 ccgaatcggc aacggattcg ccccggccga tctgggtgtc gcgggacgtc agaaacagag
     4621 cgatgccgcc gagctgatac atcccgacct cgaaagaaat tctcgtccgc gtggaggact
     4681 tgtcgaaaat cattcccagc gttttcccct tcagcgaatc gtggactttg ccggctttca
     4741 gatatttttt cagtttgccg gctttcctcc agatgatatc aaaatctttc gtgtcgagat
     4801 cataaaggct caataaatct tttttcattt cccctccaat acgctgtcaa gcaccgccac
     4861 cgtctgatca atatcctgtt cggtaatgat caggggcgga acaaaccgca acacctgacc
     4921 gcctgtggaa ttgacgagca ggccctgttt cagacattcg ccgacaatgc ccgccgcgtc
     4981 gatattcagt tttacggcca gcataagccc cttgccccgg acttcgttga tcacggcatg
     5041 cttctgctgc aattttttca attgcgatag aaaatacgcg ccggttttcc ggcaatgctc
     5101 cagcaaattc tcctgcagaa tggttttcaa tacggccgtt cccgccgcca tggccagagg
     5161 attgccgccg aacgtcgacg cgtgattgcc ggggacaaac gccttggcaa ccttgtccgt
     5221 ggccagcatc gcgcccaccg ggaaaccgtt gcccagcgct ttggccagcg tcatgatatc
     5281 cggtttaatg gcggaatatt cgtaggcaaa cagcttcccc gttctgccga tgccggtctg
     5341 cacttcatca agaatcatca gaatcccgtg ccggtcgcag atttcgcgca cccgcgccag
     5401 ataatcatca tcgggaatat tgacaccgcc ctccccctga atcggttcca gcaggacgcc
     5461 gcaggtccgg ctcgaaacgg ccttatccag cgccgacaga tcgttgaaag gaacataggt
     5521 aaacccgtcc agaagcggcg tgaaaccgaa ctgaaatttt tcctgaccgg tggccgtaat
     5581 cgtggccatg gtgcggccgt gaaacgaatc cttcatcgta atcagttcga atttatccgg
     5641 acccatattt tcatgggcgt acttgcgcgc cagtttgatg gccgcttcgt tggcctccgc
     5701 gccgctgttg cagaaaaaaa ccttgtcggc aaaagaatgc ctgatcagca gctccgccag
     5761 ctcggtttgc ggctgggtgt aatacagatt ggaaacatgc gtcagtttat ccagctgctc
     5821 cttgacggca gcaatcacct gcggatgcga atgcccgaga ttgcagacgg caatgccggc
     5881 caccagatcg agatattcct tgccgttgat atcccagacc cgggcgcccg accctttcgt
     5941 caatacgatc ggaaaccgcc tgtacgtatt catgatattt cctgtagcct gcgctatcac
     6001 ttcctgttca ttcatggtcc tgtcctgttt gtaatatttt aaagaacgat ttctgttccg
     6061 atgcccttgt cggtgaacac ctccagaagt acggcatgtt tgagccgccc gtcgacgata
     6121 tgggcttttt ggacgccgcc gttgagcgcc ttgagacagc atttgatctt gggaaacatg
     6181 ccgccttcga tcgttccgtc ctcgatcaga ttcaacgcct cgtcattggt catcgtatgg
     6241 atcaattcct ttttcttgtt caaaacaccg ggcacatcgg tcagcagcag gagcttttcg
     6301 gctttcagcg ctgccgcgat ttctccggcg acgatatccg cattgatgtt gtacgtctcg
     6361 ccgttatcgc cgagtcccgt gggcgcgata atcggaataa aattgccgtg cgccagggac
     6421 gtgatcaact gggtattgat gcttttgacc ttgcccacca ggccgatatc gataatctcc
     6481 ggcggcgtat gttttgcctt ttcttcattc agataatact tctccgccgt aatcaggttg
     6541 ccgtccttgc cgcttaatcc cacggcgttc ccgccgtgcc ggttgatcag gccgacaatt
     6601 tccttgttga ccatgccgac cagaaccatt tccacgatat tcatggtctc ctcatcggtc
     6661 acccgcattc cctggatgaa tttcgactcc ttgccgactt ttttcagaag ctcgccgatt
     6721 tgcggaccgc cgccgtgcac gaccaccgga ttgatgccga tatatttcat catcacgata
     6781 tcctgggcga accggtcttt caagtccttg tccaccatgg catgaccgcc gtatttgatc
     6841 acaatcgtct tgttgtaaaa ccgcctgata tacggcagcg tttccagaag aatatccgcc
     6901 ctttcaatcg aattagaaat attttccatt tccaatctcc atttttataa aatatacctg
     6961 ctcaaatcct gatcttcgat aatgtcgttg agtttctctt ccacatactg cgcgtcgatc
     7021 acaatgtcct ttttatccag atccggggca tcgaaggaaa tgtcatccag caatttttcc
     7081 atcatggtgt agagccttcg cgcaccgata ttttccgtat tttcattaac cactgtcgcc
     7141 atttccgcga ttgcggcaat ggcatcctcc ttgaaaacca gcctcagccc ttccgtcgcc
     7201 atcatttccc tgtactgttt caccagcgca ttattcggtt ccgtcaggat gcggatgaaa
     7261 tcctccttat ccagcgaatc cagttccacg cgaatcggga aacgtccctg aagttccgga
     7321 atcaggtccg acggcttcgt gccggaaaac gcccctgcgg cgatgaataa aatatgatcc
     7381 gttttgacca tgccgtaacg cgtgttgaca ttggagcctt ccacaatcgg cagcagatcg
     7441 cgctgcacac cttcccgtga tacgtccgga ccgtgctggg aatccccgcc gacaatctta
     7501 tcgatttcat ccaggaaaat aatgccggac tgctcgacgc gttccagcgc cgtccgggtg
     7561 accttctcca tatcgatcaa cttctgggtt tcttccgagg ccagaatttc cagcgcctcc
     7621 ggaaccttga ccgtccttcg ttttttcttc tgcggcatca tgttccccag catgtctttc
     7681 agattcagtc ccatatcctc catgccggcg gcggaaaaga tttcgatcat cggtccgctt
     7741 ctcgcttcgg tcacttccag ttctaccata cggtcgtcga atttcccttc cttgagccag
     7801 atgcgcattt tttcgcgtgt cgcatcattt ttcttttgtt ccgcctccat ggtcagaacg
     7861 gtctgttcct gcggctgatc gctgaggata tcatcaactt ttttttccgt cagccgcggc
     7921 ggcaaaagca aatccagaac cctttcctcg gcgatctccg ccgctttcgc ctgaacgctt
     7981 tcctg
//
LOCUS       DAOOLB010000086         7773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_11050_length_7773_cov_40.023840, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000086 DAOOLB010000000
VERSION     DAOOLB010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7773
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11050_length_7773_cov_40.023840"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1627)
                     /locus_tag="PLF77_09210"
     CDS             complement(41..1627)
                     /locus_tag="PLF77_09210"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC09071.1"
                     /translation="MTHLKLVEAMQKPDFYPHRPANVEFIQTHISYVFIAGDYVYKVK
                     KPLKFDFLDFTTREKRKFFCEEELKLNRRLAPDTYLDVVSLSRNEAGDITLDQGIEIV
                     DYAVRMKKLPGDRMLKTLLARGTADEKIMDALARKIVLFHQQARTDSRIDKMGDIENI
                     RHNHEENFAETEKFIDVTISEYQYSLIKDFANKFLIENKKHFEQRITDHKIRDCHGDL
                     HLDHICINDEITIFDCIEFNERFRWGDVAEDVAFLTMDIDFNGWPHHAENFIQSYLKY
                     SGDTGLLKLLNFYRCYYAYVRGKVTSLRLLQEDTTADEQRDITETAEHYFDLAYTYAA
                     RLDKPVLILTAGLMGSGKSYQARRLAVRLGTEIIRTDELRKALFNIRTTERRHERFGD
                     GIYSDDISRLVYEKALALAEQKINLGKAVIIDASFKKRTERLKALALAKKLQIRFFVL
                     ECFCPDDITQKRLEKRVLDNDNVSDGRWELFQQQKDNFEPIDEITADCRFKIDTSEKP
                     EVFRHEMIRKIKLAEFEMVK"
     gene            complement(2393..3043)
                     /locus_tag="PLF77_09215"
     CDS             complement(2393..3043)
                     /locus_tag="PLF77_09215"
                     /inference="COORDINATES: protein motif:HMM:NF016398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HPC09072.1"
                     /translation="MSQMDNIQGLITSAETDLLQEIMNIAFGRAASDLAEFIDIFVVL
                     SVPQIKLLPAYQLPDYINSEIMDYDQDRVSVVEQSFWGKFKGNALLVFPSGTGKRMIS
                     LFDGKDEFFQSDPSLEQEKETFLEIGNILIGACIGKLAELLGDIATYSPPRVLVEKSL
                     HRVYESIINPDSMAIVLRTVFSFNEKNISGFMFVLTKQESFAWLKNALHKFLEQYE"
     gene            complement(3047..3427)
                     /locus_tag="PLF77_09220"
     CDS             complement(3047..3427)
                     /locus_tag="PLF77_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC09073.1"
                     /translation="MIKKILIVDDSPISVKIIKSCVPKDMGYELYDAADGQMGVEKYK
                     EIKPDLTFMDLTMPVMTGFQALEEIMKMDTKAMVIILTADVQIKAIAKAHDLGAFSVL
                     KKPPSKESVAGAIKEAEEALASRG"
     gene            3845..5914
                     /locus_tag="PLF77_09225"
     CDS             3845..5914
                     /locus_tag="PLF77_09225"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HPC09074.1"
                     /translation="MNLNRLAISLIELNPENPADLEEFKKNLLSCYNNTQGRIQNILK
                     EACFEADAMVDQVPELQRNIIARLGVLLEQAMGSDETDISATTEQTSQVEPAREPDSI
                     DVQHVMLSADLDADLLNEFIIENMEWATMAESAILDWEQNPGNQELLNTIFRGFHTIK
                     GTSAFVNLDCVKDLAHQVESVLAKVRDGEHVFTRSYANLALKSLDVIKSILEQMKSSG
                     PGQQIELPRGYQGLLESLRNFVPDEQVQEMAIPAEESMVEPEAAAPAYTKTNRGKIAK
                     KEGEASSEQSASSNKIREQMTESTVRVKVDRLDKLLDTVGELVIAQTMVGQDNLIVSG
                     KYHELNKKISQAGKIVRELHDLSMFLRMVPLKGTFQKMVRLTRDLSLKHGKMVSCITE
                     GDDIEIDRNMVDMVADPLIHLIRNAVDHGIEFPEERRRAGKSENGRIYLTAGHAEGSI
                     MLTVWDDGRGMNRQTIVNKAIQKGLIDSAERMTDQEVWQMIFLPGFSTAEKVTEVSGR
                     GVGMDVVKQAVEALRGRIEVQTTEGQGCTFIMRMPLTMAITDGMIVKVGSQRYILPTA
                     NIHKAVRPQESDIHTVNQRAEMLSFRDEMVPVIRLYRFFDIPDSLTDLTSGLLVVIGE
                     GKQRCALFVDELLGQTQVVTKLLGKGMNNVPGIAGGAIMGDGTVGLILDASGIVSIAR
                     QQTNTTT"
     gene            5942..6475
                     /locus_tag="PLF77_09230"
     CDS             5942..6475
                     /locus_tag="PLF77_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HPC09075.1"
                     /translation="MNNANAVKNDQSANADSRAGKYLTFFLASEEYGVEILKVQEIIG
                     RMPITPVPLTSRYIRGVINLRGKIHPIMDLKIKFGMEETQITDETCIIVIKTTSMMMG
                     ILVDKVSEVINIASGDIEDTPSFGGDVNPEYLLGVGKTGGRVRLLLDIEKVITASDII
                     NMKKAAAGEGKDAMAEL"
     gene            6530..>7773
                     /locus_tag="PLF77_09235"
     CDS             6530..>7773
                     /locus_tag="PLF77_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HPC09076.1"
                     /translation="MLNDIKVGVKLIGSIVTVCLIAAAVILYGIKVRNDVVHKVDSVH
                     RQYEINIRDIGNVRVSLEKMGGDIYRYFGAPADRQKMSKEINETISSVDGTMQSYKNR
                     SIDVDEKKLIADFESAWSEMQRRYKEGMKAVDEGRQNDINQFIGAGSAAMQAREKTSA
                     TLRDLNNYSVNKNESAVKATIDNVGGLSSIMLILSAIAIALAIVTIVFLTESITKPLK
                     KGVQMMAEMKKGHLSNRLDIKRRDEIGLLANAMDEFADHLQKNIIFNMQQISEGNINV
                     APVVIDEKDEIGPALKKIIETINSLLNEVDMLTKSATEGKLDVRGNEQDYKGAYQGIV
                     QGVNRTLDAVIDPINEASAVLERIADKDMAARMVGDYKGGHAKIKKSLNLAVENLDRA
                     LQQVAIGADQVASASVQVSTGG"
BASE COUNT         2185 a         1656 c         1886 g         2046 t
ORIGIN      
        1 cccccgatgt taagctttat tccaaaggaa tccacacggg ttattttacc atttcaaatt
       61 ccgccagttt gatttttctt atcatttcat gtctgaagac ttccggtttt tcggaagtat
      121 caatcttaaa gcggcaatcc gccgttatct catcgatagg ttcaaaatta tccttctgct
      181 gttgaaaaag ttcccatcgt ccgtctgata cgttatcgtt atccagaact ctcttttcaa
      241 gtcttttttg ggtgatgtcg tccggacaga agcattccag gacaaaaaac cggatctgaa
      301 gttttttagc cagcgccagg gcctttaatc gctccgtccg ttttttaaag gaagcgtcga
      361 tgatgacagc ctttcccaga tttattttct gctcggccag cgccagggct ttttcataca
      421 ccagacggga aatatcatcg gaataaatgc cgtcaccgaa gcgctcatgc cgtctttcgg
      481 tcgtcctgat gttgaacaat gctttgcgaa gctcgtcggt acggataatt tccgttccca
      541 ggcgcacggc aagacggcgg gcttgataac ttttacccga tcccatcagc cccgcggtca
      601 gtatgagcac cggtttatcc agtcgggcgg cgtatgtata ggccagatcg aaatagtgtt
      661 ctgccgtttc ggtgatgtcc cgttgttcgt ctgcagttgt atcctcttgc agcagacgaa
      721 gactggtgac cttgccgcgg acgtaagcgt aataacaccg atagaaattc aacagtttca
      781 gcaggccggt atcaccggaa tatttcaagt aggattgtat aaaattttcg gcatgatgcg
      841 gccagccgtt gaaatcgatg tccatggtca aaaaagccac atcttcggcg acatctcccc
      901 agcgaaatcg ttcattgaat tcaatacagt cgaaaatggt gatctcatca ttgatacaga
      961 tatgatccag gtgtaaatcg ccatgacagt ctcggatctt gtgatcggtg attctctgct
     1021 cgaaatgttt tttattctcg atcagaaatt tatttgcaaa gtctttaatc aagctgtatt
     1081 gatattcgga aatggtgaca tcgataaatt tttccgtttc tgcaaaattc tcttcatgat
     1141 tgtggcgaat gttttcgatg tcgcccattt tatcgatgcg gctgtccgtt cgggcctgct
     1201 gatgaaacaa gacgatttta cgggccaggg catccattat tttttcatcc gccgttcccc
     1261 tggccagaag cgtcttgagc atcctgtcgc cggggagttt tttcatgcgc accgcataat
     1321 caacgatctc gatgccctga tcaagggtta tatcgcccgc ctcattacgt gatagcgaga
     1381 cgacatccaa atatgtgtcg ggggcaagtc ttctgtttaa tttcaattct tcttcgcaga
     1441 aaaattttcg tttctcccgc gttgtgaaat ccagaaaatc aaactttaaa ggttttttga
     1501 ctttataaac ataatcgccg gcgataaaaa cataggaaat atgcgtctgg atgaattcca
     1561 cattagccgg acgatgggga taaaaatccg gtttctgcat ggcttcgact aatttgagat
     1621 gcgtcatcgt ttatgcccgt tgtaaaataa tttctaaaat ttttttaaac aatcgcgaaa
     1681 ccataatcaa ggcaagaatc aggaagttgt cgagagtccg gtttcgcata gcggtacacg
     1741 ggattatata caaaatcgac attccgggac tgcatcgttg aatgtacgct tcaaaatttt
     1801 atgcttctta ttataccacg atatgcccta tttgatagca ttcatctgcg cgacacctcc
     1861 ggagacggag gtgcgttgac agcatgctga attaaattcc gacgtatacg ggaaaacctt
     1921 tcatgattat tttagaaccg caaaaaacgg gaaaaaggag atgttgaaga ggctgcgtga
     1981 tgtggcagaa accgtaaccg ccggaaaaat gatattccgg ttggtccgat tttattgctc
     2041 aaccttccgt ctgacgatag aaaatgaatt cgaatggatc gatcattatc tgcacagagg
     2101 aaacattctg atttgtccgt tgcatcagca attcctttcg acggtttgtt tcttttaacg
     2161 atattgatat tattatcctg tcgtcatgat ttcccaaagt caggatagat tcatgattcc
     2221 aaagccgttt gcccgtgtcc ggattttgtt cagggatgaa actgcagaaa gacgtggatg
     2281 cagacgacta tcatcacaag atgaaactgc tggggaccgt tatgaagccg ctgcgtcagt
     2341 catgaaagaa tgttccatac atcgtcttca gcaatttttg cttttcagga tgctattcgt
     2401 attgttcgag gaatttgtgc aacgcatttt tcagccaggc aaatgattcc tgtttggtga
     2461 gcacaaacat gaagccgctg atattctttt cgttaaaaga aaaaaccgtt cttaaaacga
     2521 tcgccatact atcggggttg ataatgcttt cgtaaacgcg atgtaggctt ttttcaacga
     2581 gaacgcgggg aggcgaatac gtggcaatgt cgcccaaaag ctccgcgagt tttccgatgc
     2641 atgcgccgat caatatattg cctatttcca gaaaggtttc tttttcctgt tccagcgagg
     2701 gatccgattg aaaaaattca tccttgccat caaaaagaga aatcatcctt tttccggttc
     2761 cggaaggaaa aactaaaaga gcgttgccct tgaatttgcc ccagaaactc tgttcgacga
     2821 cggatacccg atcctggtca tagtccatga tttcactatt gatatagtcc ggaagctggt
     2881 atgcaggcag caatttgatc tgcggaacgc tgagaacaac aaaaatatct ataaattcag
     2941 ccaggtccga ggcggcccgg ccgaaagcga tattcataat ctcctgaaga agatcggttt
     3001 ctgcgcttgt aatcaaaccc tgaatgttat ccatttgcga cataatttac ccacgggaag
     3061 ccagggcttc ttctgcttct ttgatagccc ctgcgacaga ttcttttgaa ggcggttttt
     3121 ttaatactga aaaagcgccc aagtcatgtg ctttggcaat ggccttgatt tgcacgtcgg
     3181 cggtcagaat gatcaccatg gcttttgtat ccattttcat aatttcttcc aaagcctgaa
     3241 atccggtcat gaccggcatg gttaaatcca taaaggtgag gtcgggttta atctccttgt
     3301 atttttccac acccatttga ccatcggccg catcgtacaa ttcatacccc atatctttcg
     3361 gaacgcaact tttaatgatt tttacggata tcggtgaatc gtcgacaatc aaaatttttt
     3421 taatcataaa aacctcttct gcgtgtagtc gttaccatat aatacttcat cggaaaagaa
     3481 gaattttatt aatttgtttt ttaacatacg gcaatataaa ataacattat attttataaa
     3541 cagattctat agattttttc aatagtgttt gtcttccggc atgaagattt ttttggatga
     3601 tgtatgatta atattttcgg aacagctatc ggcgattcgc aacggttgat tgcttgacgg
     3661 tttcggtgtg ctaatggggg gaaatactat aattataaac gaattaggaa cataacaatc
     3721 atattgacaa aaaacgtaaa aaacgtaatg attaatgaaa gccattgttt attcatggga
     3781 atactctctt ttgtgtcaaa cagattctta aaatttaatt tttgatgggg caattatctc
     3841 gaaaatgaac ttaaatcgtc tggccatatc attgatagaa ctgaatcctg aaaatcctgc
     3901 cgacttagaa gaatttaaaa aaaatcttct ttcctgttac aacaataccc aaggacgtat
     3961 tcagaatatt ttgaaagaag cttgttttga agcggatgct atggtcgacc aggttccgga
     4021 acttcagcgc aacattatag cccgtctggg agttttgctg gaacaggcaa tgggaagcga
     4081 tgaaaccgat atttctgcaa cgacggaaca aacgtctcag gtagaacctg cccgggaacc
     4141 ggacagcatt gacgtccagc acgttatgtt gtccgccgat cttgacgcgg atttattgaa
     4201 tgaattcatt atcgaaaata tggaatgggc aacgatggcc gaatccgcaa ttctggactg
     4261 ggaacagaat cccggtaacc aggaattact caatacaatt tttcgaggat tccacaccat
     4321 caagggaaca tccgcctttg tcaatctgga ttgtgtcaaa gatcttgccc atcaggtgga
     4381 atccgtgctg gccaaagtca gagatggaga acatgttttc acgagaagct atgccaatct
     4441 ggctctcaaa tcactggatg tgattaagag cattctggaa caaatgaaat catccggtcc
     4501 gggccagcag atcgaactcc cccgcggtta tcagggatta ctggaatcac tgagaaactt
     4561 tgttcccgat gagcaggtgc aggaaatggc gattccggca gaggaatcaa tggtcgaacc
     4621 cgaagcggcg gctccggctt atacgaaaac gaacagaggg aaaatagcaa aaaaggaagg
     4681 ggaggcatcg tcggaacaat ccgcttcctc aaataagatt cgcgagcaga tgaccgagtc
     4741 aacggtcaga gtaaaagtgg accggttgga taaattgctg gataccgtgg gtgaactggt
     4801 cattgcccag accatggtag gccaggacaa tctgattgtc agcggcaaat atcatgagtt
     4861 gaataaaaaa atatctcagg ccggaaaaat cgtccgtgaa cttcacgact tgagcatgtt
     4921 tttgcggatg gtgcccttaa agggcacgtt ccagaaaatg gtgcggctta cccgggacct
     4981 ttccctgaag cacggtaaaa tggtaagctg tattaccgaa ggagatgata tcgaaataga
     5041 ccgcaacatg gttgacatgg tggccgatcc tctgatccat ttgatccgca atgcggttga
     5101 ccatggcatt gaatttccgg aggaaagacg ccgggcagga aaatcagaaa atgggcgcat
     5161 ttatctgaca gccggccatg ctgagggcag cattatgctg acggtctggg atgacggccg
     5221 gggtatgaac cgtcagacca tcgtgaataa agcgatacaa aaaggattga tagattctgc
     5281 cgaacgcatg acggatcagg aagtctggca aatgatattt ctgccgggat tttcaacggc
     5341 tgaaaaggtg acggaggttt ccggccgggg tgtcggtatg gatgtggtga agcaggccgt
     5401 tgaagccctg aggggccgga tagaggttca aaccactgaa ggtcagggct gtaccttcat
     5461 catgaggatg cctctgacga tggccattac cgacgggatg atcgtcaagg tgggctccca
     5521 acgatatatt ttgccgacgg caaatattca caaagcggtc aggccgcagg aatccgatat
     5581 tcacacagtt aaccagcgag cggaaatgct gtcattccgg gatgaaatgg ttcctgtcat
     5641 cagactgtat cgctttttcg atatccctga ttcccttacc gatttaacat ccggcctgct
     5701 ggtggtgatc ggcgaaggga aacaacgctg cgctcttttc gtggatgaac tcttagggca
     5761 gacccaagtt gtgacgaaat tgctgggcaa aggaatgaat aacgtgccgg gcatagccgg
     5821 tggagcgatt atgggcgacg gaaccgtggg gttgattctg gatgcctcgg gaatcgtcag
     5881 tatcgcccgg cagcagacca atacaacaac ttaacaaaga ggccaaacag gaggagaacg
     5941 catgaacaat gcaaatgcag taaaaaacga tcaatctgcc aatgccgatt cccgggccgg
     6001 taagtatctg actttttttc tggccagtga agaatacgga gtcgaaatat taaaggttca
     6061 ggagattatc ggccggatgc cgattactcc tgtaccgctg acgtccagat atatccgggg
     6121 cgtcatcaat ttgcggggta aaatccatcc cattatggat ttaaagatca aattcggcat
     6181 ggaggaaact cagattaccg acgagacctg tatcattgtc atcaagacca ccagtatgat
     6241 gatgggtatt ctggtcgata aagtttctga ggtgattaat attgcatccg gggatattga
     6301 ggatacacct tctttcggcg gcgatgttaa tccggaatat cttttgggag tgggcaaaac
     6361 cggcggacgc gtccgcctgc tcctggatat tgaaaaagtc ataaccgcct ccgatattat
     6421 caatatgaag aaggctgctg ccggagaagg caaagacgcc atggccgagt tataggacgt
     6481 caatattcca taacgatttg aaactgacgc tacaggaagg gagataagca tgctgaacga
     6541 tattaaagtg ggagttaagc tgataggaag cattgttacc gtttgtctta tagcggcggc
     6601 agtgattcta tacggtatta aagtacgaaa cgacgtcgtg cacaaagtgg attcggtgca
     6661 tcgccagtat gaaatcaata tccgtgacat cgggaatgtc agagtcagcc tcgaaaaaat
     6721 gggaggagac atttatcgat actttggtgc accggcggat cggcagaaga tgagtaagga
     6781 aatcaatgag accatcagtt cggtggatgg cacgatgcag tcctataaaa atagaagcat
     6841 cgatgttgat gaaaagaaac tgattgccga ttttgaatcg gcgtggtccg aaatgcaacg
     6901 ccgttacaag gaaggcatga aggcggtgga tgaaggcaga cagaatgaca taaaccagtt
     6961 cataggggcc ggaagcgctg ctatgcaggc aagggagaaa acctcagcta cgcttcgcga
     7021 cctcaataat tacagtgtca ataagaatga atcggcggtt aaagcaacga ttgacaatgt
     7081 cggggggctt tcgagcatca tgctgatctt atcggcgatt gcgatagccc tggcgattgt
     7141 cacgatcgtc tttctgaccg aatccattac caaaccgctg aaaaaaggcg tgcagatgat
     7201 ggcggagatg aaaaagggac acctttccaa tcgactcgac attaaacgca gggacgagat
     7261 cggcctgctg gccaatgcca tggatgaatt tgccgatcat cttcagaaaa atatcatatt
     7321 caacatgcag cagatatccg agggtaatat taatgttgcc ccggttgtta ttgacgaaaa
     7381 ggatgagatc ggaccggcac tgaaaaaaat tatcgaaacc atcaatagtc tgctcaatga
     7441 ggtggacatg ctgaccaaat cggccacgga aggaaaactc gatgtgcgcg gtaatgaaca
     7501 ggactataaa ggagcttatc agggtattgt gcaaggcgtc aaccggacgc tggatgcggt
     7561 gattgatccg atcaatgaag cgtctgccgt gctggagaga attgccgaca aagacatggc
     7621 agcgcgcatg gtcggcgact ataaaggcgg tcatgccaag atcaagaaat cgcttaatct
     7681 ggctgttgaa aatctggaca gagctttgca gcaggtggcc atcggagcgg atcaggtagc
     7741 gtcggcaagc gtccaggtaa gcaccggcgg gca
//
LOCUS       DAOOLB010000087         7621 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_11364_length_7621_cov_34.060005, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000087 DAOOLB010000000
VERSION     DAOOLB010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11364_length_7621_cov_34.060005"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1051)
                     /locus_tag="PLF77_09240"
     CDS             complement(89..1051)
                     /locus_tag="PLF77_09240"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPC09077.1"
                     /translation="MTRPLRIQYPNAWYHVMNRGRSRAKIFHAPEDYLAFVALLREAS
                     FLWKVRIAAYCLMSNHYHLLLQTPGANLSRYMRHINGLYTQYFNRTYKSDGPLFRGRY
                     KSIIVEQDRYLLELVCYIHRNPLRAGLTKRLEDYRWTSHKAYLSESNQWNWLHKEFVL
                     SMLEENKNKQKQSYLEFMKKEESDTLKTIYEKSTLPLFLGARDFIDDIRHKYSGKTMR
                     KEMPQSREMAIDKIDVEHAVVKYYKLEVEDLLKSRRGNYNEARNVAIYLMRKHTGTKL
                     RDIGIHFGMNNYSSVGSVITRTKQEMVKNRNLRQRVQEVEKLLA"
     gene            complement(1053..1754)
                     /locus_tag="PLF77_09245"
     CDS             complement(1053..1754)
                     /locus_tag="PLF77_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09078.1"
                     /translation="MRETEDIISNLSFSHDGKNVVFDRCHEGDCQIQVYNLETGELAA
                     YQSPRNEQWTMGKYSYDGKKITFSVIPIKPNDDLDLGNMQIAVMDADGKNLKKVTTGS
                     GAKLYPTFSHNGKKILYACAARIREKGKTPAADYDAWEVGLTTGKQTQLTFFEYFYMG
                     YLTYFPDDERFIYYGDRPSVFQGLASFGNETAFGNKMIELERQGRGSRGSSLCLALVR
                     SERMLMRCENELRIN"
     gene            3067..4431
                     /locus_tag="PLF77_09250"
     CDS             3067..4431
                     /locus_tag="PLF77_09250"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPC09079.1"
                     /translation="MLKFSSIVSRVFLLFICFTALPCRADSLDYRDVITDSIGYCASL
                     RVKSEDVFISDAQYRSNFAALYPVVTLGGRAERYENIDNRDNTTINTIDNEVVGGYDS
                     AWRSSVSLSGQYAVSNWYKKIYETGYYKKLKDTSIHECEAEVKKTIRDVTDIFRSVAE
                     SKIKLMYAREILSRLNEIFRMKQAAFTNGQFSHEEILAAQTDVLNMEKDITGIHKDMR
                     ESLEKLSNYTGKSYSVDVDIIALPLSSSLPTPDETEIIAQTPEYKARLNEIEALRSRK
                     KSVSNNFLPDISVYGRYDFYNSSPDNMHDSLSDVRATDYSVGLHISLPLFDGGVRKWE
                     REKSFHELKKAQEYARLVFNEKNKDIKTLRVGYAELSKSYSYYKKINEQYEKIKDINR
                     KSKVLGERSDLDVIEFEKDALLAERDLKIIEHTMAVYEKQLALELNFNQFAGEYGGNG
                     TCRH"
     gene            4403..6508
                     /locus_tag="PLF77_09255"
     CDS             4403..6508
                     /locus_tag="PLF77_09255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HPC09080.1"
                     /translation="MAETGLVGIEVISKIHTVSIDMQAVKRRYFIETELSREEVLRIL
                     KDHGIRSRLKKLKSVDDLLKYPCPLIIISPENEFHILLGKKEDKIFIFDCNEKKPREM
                     TTDEFLKFWNGEAIAVYPRFTKTEFFLNMKWLFKEFYKHRPVFTAVITASFFIQLFGL
                     VTPLFIQVIIDKVLAHHALTTLQVVAGAFLVVLVFDTIMNLMRNYLLYHTANKVDAGL
                     GAKVYRHLLSLPFRYFETRKVGNIIARVRELENLRQFMTNISLTVLLDTVFSVVFIVI
                     MWVYSGYLTLIVLGIVFLIALISFIATPMIKQRLDEKFQKGAHNQSFLVESITGIQTV
                     KSLAIEGKMIKDWEKSLGEYIMSAFRLSNLGNVAVTSSQTLQKAMSLAVIYFGVSLVF
                     DKSMSVGQLIAFQMFASQLSGPILRLVRMWQDFQQARLSLDRIGDIINTPPEVVGGAI
                     TVKELKGEIVAKDVRFRYAPEAPLVLDGVNFTIQAGMMVGIVGRSGSGKSTIAKLIQR
                     LYLPVEGTMMVDGVDIRHMDPLFLRYRTGVVLQECYLFSGTIKENIAMAAPNAGMERI
                     IQVARIAGAHDFISEMPMGYDTYVEERGSSLSGGQKQRIAIARALIMNPNILIFDEAT
                     SSLDYESEKIIQQNLNLIRQGRTVIFIAHRLSIMRDCDMIIVIDRGKIVETGNHEALM
                     QKDGLYAYLYKQQEENRLR"
     gene            6505..>7621
                     /locus_tag="PLF77_09260"
     CDS             6505..>7621
                     /locus_tag="PLF77_09260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HPC09081.1"
                     /translation="MSKSDYHDFKPILAEIEERPTNPLGTYFLWTIISLMIIVLLTIF
                     LVKIDVVVSARGKIIPIGDVKVIQPLETGVITRIHIKEGDYVKKGDMLLEIDPSVDTA
                     DLEGKERNLSYSRLSMERINAVLNEKDFSPGKKDRASEIVRAQMTHYEAQRDVYNSTL
                     KEKEKESLENQSALESLKTEIETINKVLAITLEDEKRQKALVEIGTLADNRYREKMKE
                     RMNLERERDSKGGQVQQLETKLARISDEIQTFKSNFREKLLSDYSNNIHERNTLEAEV
                     SSLKFKQGKRFIIAPVTGYIHLLPVKTIGGVVTTAQPIVGIVPEGTPLEVNAIVMNKD
                     IGYVKEGQSCVVKVDTYDFQKYGTIEGKVEIINPYSME"
BASE COUNT         2099 a         1690 c         1704 g         2128 t
ORIGIN      
        1 tttcttcctc cgatgccgcc atagaggata tcataacctt cgccgccgtc cagcggaaaa
       61 ggggtcaagt ctttgcttgg ctcttggctc atgccaacag tttttcgact tcttgcaccc
      121 tttgccgtaa atttctattc ttaaccatct cctgtttcgt tcttgtaatc acgctgccta
      181 cgctgctgta attgttcatc ccgaaatgaa tgccgatatc tctcaacttt gttccggtgt
      241 gtttcctcat cagatatatg gctacattcc gcgcttcgtt ataattgccg cgacgcgatt
      301 ttaaaaggtc ttccacttcc aatttgtagt acttgaccac ggcatgttct acatcgattt
      361 tgtctattgc catttctctg gactgaggca tttcttttct catcgtttta ccgctatact
      421 tatgcctgat gtcgtcaatg aaatccctgg cacccagaaa caacggcagg gtactttttt
      481 catatattgt cttcaacgta tccgactctt cctttttcat aaattccagg taggactgtt
      541 tttgtttgtt cttgttctct tccagcatgg acaagacaaa ttccttgtgc agccagttcc
      601 attggttgct ttcggataga taggctttgt gggatgtcca gcgatagtct tccaggcgtt
      661 tcgtcaggcc ggctcgtagc ggattgcggt gaatgtaaca aaccagttcc agtagatatc
      721 tatcctgttc aacaatgatg cttttatagc ggcctcgaaa taacggcccg tcgcttttgt
      781 aggttctgtt gaagtattgg gtatataggc cgttaatgtg gcgcatgtag cgtgacaggt
      841 tggcccccgg tgtctgcaat aataaatgat aatgattcga catcaaacaa tatgcggcaa
      901 ttcttacttt ccacaggaaa gatgcttctc ttaataacgc tacaaaggca aggtagtctt
      961 ccggcgcatg aaatatcttc gctctgcttc tgccccggtt catgacatga taccaggcat
     1021 tggggtattg gattcttaac ggtcgtgtca tttcagttta tccttaattc attctcacat
     1081 cgcataagca ttctttctga acgaacaaga gccaagcaaa gacttgaccc ccttgacccc
     1141 ctaccctgac gttccaactc aatcattttg ttcccgaagg ccgtctcgtt accaaacgac
     1201 gctaagccct gaaatacgct cggacgatct ccataataaa taaatcgttc atcatcgggg
     1261 aaataggtca gataacccat gtaaaaatat tcaaaaaagg tgagttgcgt ctgtttgccg
     1321 gtggtcagac cgacttccca ggcatcgtag tcggcggcgg gtgttttgcc tttctccctg
     1381 atacgggccg cgcaggcata gagtattttc tttccgttat gggaaaaggt gggatagagc
     1441 ttggcgccgg aaccggtggt tactttcttt aagttcttcc cgtcggcatc catgacggcg
     1501 atctgcatat tgcccagatc aaggtcgtca ttgggcttga tgggaatgac agaaaaagtt
     1561 attttcttgc cgtcgtagga gtattttccc atcgtccatt gttcatttcg gggtgattgg
     1621 tacgccgcca gttctcccgt ttccagatta tagacttgta tctggcaatc gccttcatgg
     1681 cagcggtcaa aaacaacatt cttcccgtca tgggaaaagc ttaaattact gataatatct
     1741 tctgtttctc gcatggcaaa acaacctcct atggatgtca tcatgtttat aaaaatgaat
     1801 gttgttatac agtataaaat tactctgctg attatctgct gaaaattaga tttagagata
     1861 ttgaaaatga atctattgat gttttcgttg tacctttttt tcattccgcc ctccttaatt
     1921 tttcgcgcaa tgaaacacgc gaccttccag gcagtccaag gggtcaagtc tcctcttggc
     1981 tcatggcaat attgcctcca cgtccgctat tcttttccgc aaacctacat cctctcttaa
     2041 tgcctgttcc atcctttgaa taacactgct cacgctgctg taactgcgca ttacgaaccg
     2101 attcccaatt tctgatagcg aagctccggt atgttttctc attaaatatg tccataatgg
     2161 gacggttcta ttttttgaaa taagttcctt attttttctc attaattcct ccttcatttt
     2221 tcgcataaag atactcgcag tcctgtagcg acacagttac gttccaaaac aaatgtttta
     2281 ttatgattaa ttattcgcgg aataatatta gaaatgcttc ttggattacc atggtattcc
     2341 gaatgataaa ctgaggtaag aaaacgatca gcatagcgaa tcacataaca attctgttga
     2401 tcaaaaggaa ctttcactct ttttccttag catgaaccgt ttaaagcagt tcatcctttt
     2461 gatttgaaca accgtgaatc agtgaaactt ttcttgtgaa gctttttgat tctataagat
     2521 tctataagac gcgaatttaa taaataaaaa ttatcttctt gtcaataaaa ttcattaaaa
     2581 atgatatttg ttttatcaag aatgatgtta tctttagccg aaatgaaaat gatagaaaaa
     2641 aaagtggcga gaaaaggctt ctgaatttac cctatatgat ctgtgagtcg cacttcgccc
     2701 gatcatcgtg ggaaatgaca aaatgaagtg tttcgatttt ttacatgatt gtgtaaatta
     2761 gtcttgactt ttttacatat agaaacggga ttttatttcg gcaaacaact gtcagtgccc
     2821 tattttcaga ttgttcgaaa ctgccgaaaa gccgaagaat ttcccggcaa ccgcgccgga
     2881 attccctcaa cgcatttcct gatgataacc ggataaatat tgatcaggga cagaggccta
     2941 tttgtttagc aaattttatt tgcatccttg attgcaaatt gatatatgca gcgcgattgt
     3001 cgcagtcgag gcaatatttt tccatttaca gcagcaaaaa taaacatagt tagacagtgt
     3061 gaataattgt taaagttcag cagcattgtt tcacgcgttt ttttattgtt catttgcttt
     3121 acagctcttc cctgccgggc tgattctctg gattaccgcg atgtgatcac cgacagcatc
     3181 ggttattgcg cgtctctgcg tgtcaagagt gaggatgttt ttatttccga tgcgcaatac
     3241 cgaagcaatt ttgccgctct ctatccggtc gtcaccctcg gcgggcgcgc ggaaagatat
     3301 gaaaatatcg ataatcgtga caacacgacg attaacacga tagacaatga ggtggtcggc
     3361 ggttatgata gcgcgtggag atcgtccgtt tctttatcgg gacagtatgc cgtttccaac
     3421 tggtacaaga aaatttatga aacgggctat tataaaaaac tcaaggacac gagcatccat
     3481 gaatgtgaag cggaagtaaa aaaaacgatc cgggatgtta cggatatttt ccgctctgtc
     3541 gcagaaagca aaatcaagct gatgtacgcc cgtgaaattc tgtcgcgtct taacgaaatt
     3601 ttcaggatga aacaggcagc tttcaccaac ggacaatttt ctcatgagga gattctggcg
     3661 gcacaaactg acgttttgaa tatggaaaag gatattaccg gcattcacaa agacatgcgc
     3721 gagagcctcg agaaactgag caactatacc ggcaaatctt attccgtcga tgtggatatc
     3781 attgctttgc cgctgtcatc ctccttgccg acaccggacg aaactgagat tattgcacaa
     3841 acccccgagt ataaagcgcg gctgaacgaa atcgaggccc tcagatccag gaaaaaatcg
     3901 gtgagcaata attttctgcc ggatatttcc gtttacggcc gatacgattt ttacaacagc
     3961 agccccgaca atatgcatga ttcgctcagt gatgtccgtg cgaccgatta cagcgtcgga
     4021 cttcacatca gcctgccctt gtttgacgga ggcgtccgta aatgggagag ggaaaaatct
     4081 tttcatgaac tgaagaaggc gcaggaatac gcccgcctcg ttttcaacga aaaaaataag
     4141 gacatcaaaa cgctccgggt cggatacgcc gaactttcga aatcatactc gtattataaa
     4201 aaaatcaacg aacaatatga gaaaattaag gacatcaaca gaaaatcaaa agttctcggc
     4261 gaaagaagcg atcttgacgt tattgaattt gaaaaagacg ctctgctggc ggaacgcgat
     4321 ctgaaaatca tcgagcacac aatggctgtt tatgaaaaac aactggcgct cgagttgaac
     4381 tttaatcaat ttgcaggtga atatggcgga aacgggactt gtcggcattg aagttatctc
     4441 caagatccac acggtaagca tagacatgca agccgttaaa cggcgctact ttatcgaaac
     4501 cgaactgagc cgggaggagg ttttgcgtat cctgaaggat cacggcattc gttcgcgtct
     4561 gaagaagctg aaatcggttg atgacctgct gaaatatccg tgtcccttga tcatcatctc
     4621 tcccgaaaac gaattccata ttctcctggg gaaaaaggaa gataagatct tcatcttcga
     4681 ctgcaatgag aaaaagcccc gggaaatgac aaccgatgaa tttcttaaat tctggaacgg
     4741 cgaggccatc gcggtgtatc cccgttttac gaagacggaa ttttttctga acatgaaatg
     4801 gctctttaaa gaattctata aacaccgtcc ggtttttacc gccgtcatta cggcttcttt
     4861 tttcattcag ttgttcggcc tggtcacgcc gttatttatc caggtcatta ttgacaaggt
     4921 ccttgcccat cacgcgttaa ctacgctaca agtcgtcgcg ggagcttttc tggttgtgct
     4981 ggttttcgat accatcatga acctgatgcg caattacctc ctttatcata ccgccaacaa
     5041 ggttgacgca ggactcgggg caaaggttta ccgtcatctg ctttcgctgc cgtttcgtta
     5101 ttttgaaacc cgaaaagtgg gcaatatcat cgcccgtgtc cgcgaactgg aaaacctgcg
     5161 gcagttcatg accaatatct ctcttaccgt tttgctcgat acggtgtttt cggtggtgtt
     5221 tatcgtcatc atgtgggttt acagcggata tttgacactc attgtgctgg gcattgtttt
     5281 tcttatcgcg cttatttcat ttatagccac accgatgatc aagcaacgat tagatgaaaa
     5341 attccagaaa ggcgcgcata atcaatcttt cctggtagaa tccattaccg gtattcaaac
     5401 cgtaaaatcg ctggctattg aaggcaagat gattaaggac tgggaaaaaa gcctcggcga
     5461 atacattatg tccgcgttca ggctgtccaa cctgggcaat gtggcggtaa cgtcttcgca
     5521 aacgctgcaa aaggccatgt cgcttgccgt tatttatttc ggtgtcagcc ttgttttcga
     5581 taaaagcatg agcgtagggc aactgatcgc ttttcagatg ttcgcatcgc aactgagcgg
     5641 accgatcctg cgcctggtgc gtatgtggca ggactttcaa caggccaggc tttcactgga
     5701 cagaatcggc gatatcatta atacaccgcc ggaagtggtc ggcggcgcca ttacggtcaa
     5761 agaactgaaa ggtgaaattg ttgccaagga tgtccgtttc cgctatgcgc cggaggcacc
     5821 gttggtgctg gacggtgtca atttcactat ccaagcaggt atgatggtgg gtattgtcgg
     5881 acgaagcggt tccggcaaaa gcacgattgc caaactgatt cagcggcttt atctgccggt
     5941 cgaaggcacc atgatggtcg acggcgtgga tatccgtcac atggacccgc tgtttctgcg
     6001 ctatcgcacc ggcgttgttt tgcaggaatg ttatcttttc agcggcacca ttaaagaaaa
     6061 tattgccatg gcggcgccca acgccggcat ggaaagaatc atccaggtgg cgcggattgc
     6121 cggcgctcat gattttattt ccgaaatgcc catggggtac gatacttatg tcgaggaaag
     6181 aggctcttca ttgtccggcg gccaaaagca gcgaatcgcc attgcccgcg cgctgattat
     6241 gaatcccaat attttaattt tcgacgaagc aaccagctct ctggattatg aatcggaaaa
     6301 gatcatccaa cagaacctga acctgatcag gcaagggaga accgttatat ttatcgcgca
     6361 caggctttcg atcatgcgcg attgcgatat gattatagtg attgatcggg gcaaaattgt
     6421 ggaaaccggc aatcatgaag ctctgatgca aaaagacggc ctttacgctt acctctataa
     6481 acaacaggag gagaacagac tgagatgagc aaatccgatt atcacgattt taagcccatt
     6541 ctggcggaaa ttgaagaacg gccgacaaat ccgctgggca cctattttct gtggactatc
     6601 atatcgctga tgattattgt tctgctaact atatttcttg ttaaaatcga cgttgtcgtt
     6661 tcagcacgcg ggaaaatcat cccgataggg gatgtcaagg tgatacagcc cctggaaaca
     6721 ggtgtcataa cccgcataca tattaaagaa ggagactacg tcaagaaagg agatatgctg
     6781 ctggagatag atccttccgt ggatacggcg gatctggagg ggaaggaaag aaatctgagt
     6841 tattcccgcc tgtccatgga acgaatcaat gccgtgctta acgaaaaaga tttttcgccg
     6901 gggaaaaaag atcgcgcatc ggaaattgtt agagcacaga tgacacatta tgaggcgcaa
     6961 agagatgttt ataattcgac tttgaaagaa aaagagaaag agtctctcga aaatcaaagc
     7021 gcgctggaat ctcttaaaac agaaatcgaa acgataaaca aagtcctggc catcacgctg
     7081 gaagatgaaa aaagacaaaa agctttggtg gaaatcggta cgctggctga taatcgctac
     7141 cgggaaaaaa tgaaggaaag aatgaatctg gaacgcgaac gcgattccaa aggaggccag
     7201 gttcaacagt tggaaacaaa gctcgccaga ataagtgatg agatacaaac cttcaaaagc
     7261 aatttccggg aaaagctgct ttccgattat tccaataata tccatgaacg aaatactttg
     7321 gaagcggaag taagcagtct gaaattcaaa cagggcaaga gatttatcat cgcgccggta
     7381 accggctata ttcacctgtt gccggtcaaa accatcggcg gggtcgtcac aacagcacag
     7441 ccgatcgtcg gtatcgttcc ggaaggcaca ccgctggaag tcaatgccat cgtaatgaat
     7501 aaagacatcg gctatgtcaa agaggggcaa tcctgtgttg tgaaggtgga tacgtacgac
     7561 tttcagaaat acgggaccat tgaaggcaaa gtcgaaatta tcaatcctta tagtatggaa
     7621 g
//
LOCUS       DAOOLB010000088         7327 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_12021_length_7327_cov_40.708058, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000088 DAOOLB010000000
VERSION     DAOOLB010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12021_length_7327_cov_40.708058"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PLF77_09265"
     CDS             <1..667
                     /locus_tag="PLF77_09265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPC09082.1"
                     /translation="SWGFSCLIQHEGQNILFDTGGDGLVLAANMAKLGIDPASLDLLM
                     ISHQHWDHTGGIYYILKEKRNLRICLPHSFSARFKEDMKRYGAEVIDINEAREVITGL
                     HSTGDMEALIREQSALLKTQAGTVVITGCAHPGIVHIAETAKRILPDDALALVMGGFH
                     LLDNADEDILKIIARFRELGVRYAAASHCSGPNARELFARKYGEHFIRLGAGSTIALE
                     DLR"
     gene            complement(701..1312)
                     /locus_tag="PLF77_09270"
     CDS             complement(701..1312)
                     /locus_tag="PLF77_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HPC09083.1"
                     /translation="MASQALHKKFKQGDLLIGEEDPIRAFYVVTAGQLKLYKSSSEGR
                     EQTLSLLKPGDPFGLCTAFATESFPANAMAIEKSAVLIIPGELMENVAKKEPVLLLNI
                     IQILSQRLKESMTLIESLSLKEIPARLSTYILDALSRTSEDKRNKLELTISHRELSKM
                     LGATPEALSRAFKKMSNDKILKVEGRVITVLNREEMKTLAEGD"
     gene            1521..1853
                     /gene="trxA"
                     /locus_tag="PLF77_09275"
     CDS             1521..1853
                     /gene="trxA"
                     /locus_tag="PLF77_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPC09084.1"
                     /translation="MNKQDIKHAGDGDFEKTVLKNDKLVLVDFWAPWCGPCRAIGPIL
                     EELAGEYGDRIDIVKVNVDDNPQTASQFGIKSIPTLLVMKGGTVKDTSIGLSSKDKLA
                     DMIKRHIH"
     gene            2266..2661
                     /gene="rsfS"
                     /locus_tag="PLF77_09280"
     CDS             2266..2661
                     /gene="rsfS"
                     /locus_tag="PLF77_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPC09085.1"
                     /translation="MPAKKNVTRERLILCINAVLQKKAKDIIVLNLSKLSAFTDYMII
                     CSGTSEREVQAIAQAIQLFLKKADIRPLGVEGEANGQWVLLDYDDVVISVFYEPFRTF
                     YDLENLWDAPRMQVDENKKEIKRLSREMA"
     gene            2658..3401
                     /locus_tag="PLF77_09285"
     CDS             2658..3401
                     /locus_tag="PLF77_09285"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HPC09086.1"
                     /translation="MNLAEAFNDLSQVIFPPQCLGCAEIIPSVKNQIFCRDCAKHVHF
                     IAGSICPVCGTTFPGSPAENHLCEDCLKKQPYFSQARAVFCYEAVILDAIHQFKYKRD
                     IATGEILADFMADYPFPDIAFDDYSLIVPVPLHVKRLRERGFNQSLILARRLAKRKQI
                     PVDFLVLKRHRFTSTQTQSNKQERKINIRGAFEVRGIKTIAGRNILLIDDVFTTGATV
                     NECSKTLMKAGAKKVSVLTLARVLQHVSF"
     gene            3419..3913
                     /gene="rfaE2"
                     /locus_tag="PLF77_09290"
     CDS             3419..3913
                     /gene="rfaE2"
                     /locus_tag="PLF77_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPC09087.1"
                     /translation="MNKILDRTTLQRKLDTLRQKGRKIAFTNGCFDILHVGHVRYLRE
                     ASKTGDVLVLALNSDSSVRAIKGEKRPLVPATERAEILAALDFIDFITIFDELTPLDV
                     IKSLKPDILIKGGDWPEEKVVGREEVKKWKGKVVIIPEVQGKSTTNIVEKILQVYGPQ
                     NQKG"
     gene            4042..4428
                     /gene="dksA"
                     /locus_tag="PLF77_09295"
     CDS             4042..4428
                     /gene="dksA"
                     /locus_tag="PLF77_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HPC09088.1"
                     /translation="MEKQINVKQKQEKMKAYKAMLTQKINELLSEAGKTVSEMTNGKE
                     NYPDPTDRASLESDRNFELRIRDRERKLIMKMQEAIKRIDDGTFGICEVCGGPISEKR
                     LMARPVTTLCIDCKTKQEKMEKLKGE"
     gene            4604..7012
                     /gene="priA"
                     /locus_tag="PLF77_09300"
     CDS             4604..7012
                     /gene="priA"
                     /locus_tag="PLF77_09300"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPC09089.1"
                     /translation="MYVEIALNIPSDKLFSYEVPTELEQDIVVGKRVFVPFGNKKRTG
                     FITDIRHSCGLNNIKSVAEVLDDEPLFHTSDLNFYKWIAGYFIYPLGKTLAELIPAGP
                     EKKDFLWITPLISETDLHLTPAQNTLMAFLKQHPRGTTLTGIAKISGLKNALSAAKKL
                     QQAGLVSLEKKHGKPLSPQSEKMVALDEQKLPDVKLTGKQRMLVEWMQAKGSVSWGEL
                     IEGSGVTGAVLKALLDKGLIHLTKQEKIRTASFDSHIGRHKNATVLNDRQNAAMEEIS
                     RYLEKNIFTPILLHGVTGSGKTEIYLKAIENVLQTGGSAMYLVPEIALTPQLISRVAG
                     RFDRERIAILHSGIAESVRYDQWRQIRRGRIHLVIGTRSALFAPLTNLRLIIVDEEHD
                     TSYKQDDRLCYNARDLAVVKAKMSSAVVIAGSATPSIHTYFNAQTQKYHYLELPGRID
                     DKPMPVVEIVDMKLQQAKAGKVPVLSDSLIEQIDNTVRNKEQVLLFLNKRGFDTFLVC
                     ADCGYSFRCPNCAVSLKNHLAEGIVKCHYCDYTIQSMPLCPSCKSSRILSYGTGTQKL
                     EKEVQKFFPDARIDRMDSDTTTRKGSQEKILQALDKREIDILIGTQMITKGHDFPFIT
                     LVGVVSADTSLNMPDFRAAERTFQLITQVAGRGGRGSAPGRVIIQTFNPDHYALRHAQ
                     NHDYKSFYREEIKFRLDLQYPPFGRIINLRLSSIKKDVLIEEAKRLGKLAKKLCAGYG
                     NTVEIIGPAESPLSKLRGRFRWQMLIKGKDINALHQIAKDVMHKNKNTQIKMMADVDP
                     ESFM"
     gene            complement(7022..>7327)
                     /locus_tag="PLF77_09305"
     CDS             complement(7022..>7327)
                     /locus_tag="PLF77_09305"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPC09090.1"
                     /translation="TELSNSADDPDVSIARARVEPGVTTRSHRLKGITERYFIISGRG
                     AVEIGELPPQEVNPGDIVLIPPLCPQRITNIGKNDLIFLAICSPRYMQDAYEDVEDF"
BASE COUNT         2088 a         1712 c         1743 g         1784 t
ORIGIN      
        1 ctcgtgggga ttttcctgtc tgatccagca tgaagggcaa aacatccttt ttgataccgg
       61 cggcgatggt cttgtcctgg cggcaaatat ggcaaagctg ggcattgatc cggcatcgct
      121 tgatcttctg atgatctctc accagcattg ggatcatacc ggaggcatct attatattct
      181 gaaagaaaaa cgaaatctcc ggatttgtct gcctcattcg ttttccgccc gcttcaaaga
      241 agatatgaag cgctacggcg cagaagtgat tgacataaat gaagcacgag aagtgatcac
      301 cggtctgcac agtaccggcg acatggaggc cctgatccgg gaacagagcg cccttctgaa
      361 aacgcaggcg ggaaccgtcg tgattaccgg atgtgcgcat ccgggcatcg tgcatattgc
      421 cgaaaccgcg aagcgtattt taccggatga tgcactggcc ctggtcatgg gaggttttca
      481 tttgctcgac aatgctgacg aagacatttt gaaaatcatc gcccggttcc gggaactcgg
      541 cgtccgttac gcggccgcct ctcactgcag cggtcctaat gcccgcgagc tcttcgcccg
      601 caaatacgga gagcatttca ttcgtctcgg cgcaggcagt acgatcgctc tggaggattt
      661 gagataaccc cggatcatca atgtttagca ctggaataac ttaatcgccc tcggccagcg
      721 ttttcatctc ttccctgttc aaaacggtaa tgacgcgtcc ctcgaccttc agaattttat
      781 cgttgctcat tttcttgaac gcacgggaaa gcgcctcggg cgttgcgcca agcatcttgg
      841 ataattcccg gtgggatatg gttaattcca gtttgtttct cttgtcttct gacgtccttg
      901 aaagtgcgtc aagaatgtag gttgacagcc ttgccggtat ttccttcagc gaaagcgatt
      961 cgatcagcgt catggattcc ttgaggcgct gggaaagtat ttgaatgatg ttgagaagca
     1021 gcaccggttc ttttttagcg acattctcca tcaattctcc cggaataatc aatactgcgc
     1081 ttttttcaat agccatggca ttggcgggaa agctttccgt ggcaaaggct gtacaaagac
     1141 cgaaaggatc tcccggtttt aaaagcgata acgtctgttc cctgccctcg gatgaacttt
     1201 tgtaaagctt taactgcccg gcggtcacca cgtaaaacgc ccggatggga tcttcctccc
     1261 cgatcagcaa atcaccctgt ttgaattttt tatgcaacgc ctgcgatgcc atgagtttca
     1321 agttctccgc cgacaggcct tgaaacagcc caaagttctg tatttgttta agaagttcca
     1381 tggcaaatat ttttctccac attgattcag atcaatgaca ttccacaaac cgggacatat
     1441 gcttgcgatt atgaatatac gaatatttaa caattgcgga ttgataaatc aaatactttt
     1501 atatcaaggg aggggagcaa atgaataagc aggacatcaa gcatgccggt gacggtgatt
     1561 tcgaaaaaac cgttttaaaa aatgacaagc tggttttagt ggatttctgg gcgccgtggt
     1621 gcggaccttg ccgtgcgatc ggcccgattc tggaggaact ggccggagaa tacggcgacc
     1681 ggatagacat tgtgaaggtt aacgtggatg acaatcctca aaccgcgagt caattcggta
     1741 tcaaaagcat cccgacactg ctggtcatga aagggggaac ggtaaaggac acgtcgatcg
     1801 gtttgtcctc gaaggataaa ctggccgata tgattaaacg gcatatccac tgataacacc
     1861 agggggcaaa ggaaatgaat gattttttga tattggccgt cattgttgcg gtatggtttt
     1921 tgttgcaggg atggctcttg cctaaattgg gtgtatccac ctgaatgaaa cccggctgtc
     1981 cggtcgagga cagaaagaaa gaaaaaacat caaccgattc ttaacacgaa agcagaaact
     2041 gctttaaacg ggttaatatt gtttcataaa caacgtcagg gcctgtttct cgaatcggac
     2101 gcaaggggtt catcctgtgt aaaaacatgt gcaaagaaaa cagaaactgt cctgaatacc
     2161 ccatgacgat tttttataat cttcatgcaa aaaaacagca aagttgtcat tttccgtcat
     2221 ttgtggcata tattcggaaa attcagtgcc ggacaggagg tttatttgcc agcaaagaaa
     2281 aacgtgacca gagaacgcct gattttgtgt attaacgctg tattacaaaa aaaagcaaaa
     2341 gacatcattg ttttaaattt aagcaaactt tcagctttca cagattacat gatcatctgc
     2401 agcggaacgt cggagaggga agtccaggct atcgcccagg ccattcaatt attcctgaaa
     2461 aaagcggaca tcagacctct gggcgtcgaa ggcgaagcca acggccaatg ggttctgctg
     2521 gattacgacg acgttgtcat ttccgttttt tatgaaccgt tcagaacgtt ttacgatctt
     2581 gaaaatctct gggatgctcc ccggatgcag gttgacgaga acaaaaagga aataaaaagg
     2641 ttaagcagag aaatggcatg aatctggctg aagcgttcaa tgatttaagc caagtaattt
     2701 ttccgcctca atgtctggga tgcgctgaaa tcatcccgtc cgtgaagaat cagatttttt
     2761 gtcgggattg cgctaaacac gtccatttta ttgccggcag catctgtccc gtttgcggca
     2821 caacctttcc cggcagtccg gcggagaacc atctgtgcga agattgtctg aagaaacagc
     2881 cttatttttc tcaagcgagg gccgtctttt gttacgaagc ggtgatcctc gacgcgattc
     2941 atcaatttaa atacaaacgg gatatcgcta caggggaaat tctggcggat ttcatggccg
     3001 attatccttt tccggacatt gcttttgacg attattcgct gattgttccc gttcctctgc
     3061 atgttaaaag actgcgcgaa agagggttca atcaatcgct tattctggcc cgccgtctgg
     3121 cgaagagaaa acaaattcct gttgacttct tggtgttgaa acggcacaga tttacgtcaa
     3181 cccagactca gtcgaataaa caagaaagaa agataaatat cagaggagct tttgaagtca
     3241 gagggataaa aacaattgcc ggacgcaata tcctgctcat cgacgacgtc tttactaccg
     3301 gcgccaccgt taatgaatgt tccaaaaccc tgatgaaggc cggagcaaaa aaagtttccg
     3361 tattgaccct ggccagagtg ttacaacatg tctcatttta aaacgtaaag gagcggtaat
     3421 gaataaaatt ttagacagga caaccctgca aagaaagctg gatacactgc ggcaaaaagg
     3481 aaggaaaatc gctttcacta acggatgttt tgatattctg catgtcggtc atgtccgcta
     3541 cctgcgcgaa gcaagcaaaa ccggcgatgt gctggttctg gctttaaaca gcgattcgtc
     3601 agtcagagcc atcaaaggag aaaaaagacc tctcgttccg gcaacagaaa gagcggaaat
     3661 ccttgccgcg ctcgacttca tcgactttat tacaatattt gatgaattga cgcctctgga
     3721 cgtcatcaaa tccctgaagc cggacatcct gatcaaggga ggagactggc cggaagaaaa
     3781 agttgtcggc cgcgaagaag ttaagaaatg gaaaggcaaa gttgtcatta ttcccgaagt
     3841 gcagggtaaa tccaccacca acattgtgga aaagatcctg caggtttatg gtccgcaaaa
     3901 ccaaaagggt tgattttatt catactatca ttgaattgag gcatattgcc tgaaaaaact
     3961 gcggacaaaa atttctcttt acaaataaat tttcatcatg tagatttgac ggccaagttg
     4021 ttcttgcaag gagtgatgat tttggaaaaa caaataaatg taaaacagaa acaggaaaaa
     4081 atgaaggctt acaaagccat gctgactcaa aaaatcaacg agttgctgag tgaagccggc
     4141 aaaactgttt cggaaatgac caacgggaaa gaaaactatc ccgatcccac cgacagggcg
     4201 tctcttgaat ccgacagaaa ttttgaattg cgtatccggg acagagaacg caaactgatc
     4261 atgaaaatgc aggaagccat taaaaggatc gatgacggca ctttcggtat ctgcgaagtc
     4321 tgcggcggtc ccatatcaga gaaaagattg atggcaagac cggtcacgac cctctgtatc
     4381 gattgtaaaa caaaacagga aaagatggaa aagctcaaag gcgaataaat ttaaaatatt
     4441 ccgcttgata aaaaacgctc cccaaccgga gcgttttttt atttttaaaa caatagctac
     4501 cgataaataa tttccttttt ggactaaaga tggtatcaga aaaatggatt tcccggacag
     4561 gatcggttac taacggtata tttttgaact tcatggaata gttatgtatg tagaaatcgc
     4621 cttaaacatt ccatcggata aactcttctc gtatgaagtt cccactgaac tggagcagga
     4681 cattgtcgtc ggcaaacgcg tctttgttcc tttcggcaat aaaaaacgca ccggattcat
     4741 aaccgatatc cgtcattcct gcggcctgaa caatatcaaa tccgttgcgg aagtgcttga
     4801 cgatgagccg ctgtttcata caagcgacct gaacttttac aaatggattg ccggctattt
     4861 tatttatccg ctgggtaaga ccctggccga gctcattccg gcaggaccgg aaaaaaaaga
     4921 ttttctctgg ataaccccgc tgatatcgga aaccgatctg catttaacgc ctgcacaaaa
     4981 tacactgatg gcgttcctga aacaacatcc gcggggcacg acgctcaccg gcatcgcgaa
     5041 aatcagcggc ctgaaaaacg ctctttcggc ggcaaaaaaa cttcagcagg cgggattggt
     5101 ctctctggag aaaaaacacg gcaaaccgtt gtccccgcaa agcgaaaaaa tggttgcgct
     5161 cgacgaacaa aaactgcccg acgtcaaatt aaccggcaaa cagcgaatgc ttgtcgaatg
     5221 gatgcaggca aaaggctcgg tatcatgggg agaattgatt gaaggttccg gcgttaccgg
     5281 cgccgtgctc aaagccttac tggataaagg tctgatccat ctgacgaaac aggagaaaat
     5341 ccgaaccgct tcattcgatt cgcacatcgg acgccataaa aacgcaaccg ttctcaacga
     5401 ccggcagaat gcggcaatgg aagagatttc acgctatctg gaaaaaaata ttttcacgcc
     5461 catactgctt cacggtgtaa ccggcagcgg gaaaacggaa atctatttaa aagccattga
     5521 aaatgtttta caaactggcg gctctgccat gtatctcgta ccggaaatag ccctcacgcc
     5581 tcagctgatt tcgcgggttg ccggacggtt tgaccgggaa cgcattgcca tactgcacag
     5641 cggcatcgcc gaatccgtac gctatgacca gtggcggcag atcagacgcg gtcgcattca
     5701 tctggtaatc ggcacccgtt ccgctctttt cgctccgctg accaatttga gattgatcat
     5761 cgtggatgaa gaacacgata cttcttacaa gcaggatgac cggctctgct ataatgcccg
     5821 ggacctggcc gtcgtgaagg caaaaatgtc ttccgccgtt gtcatcgccg gttccgctac
     5881 gccgagcatt catacgtatt tcaacgcgca gacccaaaaa tatcattatc tggaacttcc
     5941 cggaagaatc gatgacaaac cgatgcctgt cgtcgagatt gttgatatga aattacagca
     6001 ggcgaaagcg ggaaaagtgc cggtgctttc ggacagtttg attgagcaaa tcgacaatac
     6061 cgtccggaac aaagaacagg ttctgctttt tctcaacaag cgcgggtttg atacattttt
     6121 agtttgcgcc gactgcggat atagtttccg ctgtcccaat tgcgccgtct ccctcaaaaa
     6181 tcatcttgcg gaaggcatcg tcaaatgcca ttactgcgac tatacgattc aatcgatgcc
     6241 gctttgccct tcctgcaaaa gcagccgcat tttaagttac ggaacgggca cgcagaaact
     6301 ggaaaaagaa gtgcagaaat tctttcctga cgcccggatt gacagaatgg actccgacac
     6361 gaccacccgt aaagggtctc aggaaaaaat tcttcaggca ctggacaaaa gggaaattga
     6421 cattctcatc ggcacgcaaa tgatcaccaa aggacacgac tttccgttca ttaccctggt
     6481 gggcgttgtt tccgcggaca cttccctgaa catgccggat ttccgcgccg ccgaaagaac
     6541 gtttcagtta atcacgcaag tcgccggacg aggaggacgg ggaagcgcac ccggccgagt
     6601 cattattcaa acgttcaatc ccgaccacta tgcgctgagg cacgctcaaa accatgatta
     6661 taaatccttt tacagggaag aaataaaatt tcgtctggat ctgcaatacc cgccgttcgg
     6721 ccggatcatc aatttgcgcc tgtcttcgat caaaaaagac gtattgattg aggaagccaa
     6781 acgtctgggc aaactggcga agaagctgtg cgcggggtat ggaaacacgg tagaaattat
     6841 cggtccggcg gaatctcccc tttccaagct ccgggggcgc ttccgctggc agatgctgat
     6901 caaagggaaa gacatcaacg ccctgcatca gatcgcaaaa gacgttatgc ataaaaataa
     6961 aaacacgcaa ataaaaatga tggcggatgt ggacccggaa agcttcatgt aatcatcagg
     7021 gttaaaagtc ttcgacatct tcatacgcat cctgcatata acgcggcgag cagatggcca
     7081 gaaaaatcaa atcgtttttg ccgatgttgg taatgcgctg cggacataag ggcggaatca
     7141 agactatatc gccggggttg acttcctggg gcggcagttc tccgatttcc acagctcccc
     7201 tgccgctgat gataaaataa cgttccgtaa ttcctttcag ccgatgactg cgcgttgtca
     7261 cgcccggctc cacccttgcc cgggcaatgg atacatcagg atcgtcggca gaattggata
     7321 attccgt
//
LOCUS       DAOOLB010000089         7263 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_12154_length_7263_cov_40.717814, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000089 DAOOLB010000000
VERSION     DAOOLB010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12154_length_7263_cov_40.717814"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /gene="bamD"
                     /locus_tag="PLF77_09310"
     CDS             complement(<1..1046)
                     /gene="bamD"
                     /locus_tag="PLF77_09310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPC09091.1"
                     /translation="MHAKKFVKTAPLKSVLTLLLLSLILGGCSSFSLFGKRDLPQASP
                     EGLYATAAEEYKDGNYKKAREYFSRVKEEYPLHELSVLAEIGIADSLYSNKDYEEAKD
                     AYSDFISLHPINENVPYAVYQTGMCHYHRMEAIDRDQTETISARKEWEKLIARYPESK
                     FSIMAEKMLKEVKQRLAEREFYIGNFYLNHKKYKAALARFEKIAAEYSNVGLDYKIEN
                     YIKEAKTGVDEEEKEKLREDEVQKSKEQDTIKKEEEKKKIAEEKRLKEEEAKRVKEEA
                     RIKKEEEKKRKVEEKRLQEEEAKRVKEEARIKKEEEKKKKAEEKRLREEEAKRVKEEA
                     RIKKEEEKKRKAEE"
     gene            complement(1078..1407)
                     /gene="trxA"
                     /locus_tag="PLF77_09315"
     CDS             complement(1078..1407)
                     /gene="trxA"
                     /locus_tag="PLF77_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPC09092.1"
                     /translation="MSSELLAAATDSNFENEVLKSDKPVLVDFWAPWCGPCKAIGPIV
                     EEIAGQYKDSVKIMKLNVDDNQKMASHFGVLSIPTLILFKEGKVLEKIVGLVPKARLE
                     DLIKKAL"
     gene            1552..2262
                     /locus_tag="PLF77_09320"
     CDS             1552..2262
                     /locus_tag="PLF77_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTAP family purine nucleoside phosphorylase"
                     /protein_id="HPC09093.1"
                     /translation="MGMLGIISTRLVLEQSELLKKSSAKPMENGFGKTTVFTTDKTAL
                     ILRHGMDPDNYILPHLINHPANLQALKDIGVSEIVSINSTGSLKKEIKPGMIVVPDDF
                     LTLTPSPTIHVNRPVHIIPSLNEGIRKKLIRAALKNDFEVVQQGTYWQTQGPRLETKA
                     EISTMTRFADIVGMTMANEAVIAQEMDIPYACACSVDNFGNGLMAEPLSMEEIISHTR
                     KNAQRMIQLLDRYLQMFF"
     gene            complement(2264..2698)
                     /locus_tag="PLF77_09325"
     CDS             complement(2264..2698)
                     /locus_tag="PLF77_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09094.1"
                     /translation="MTHEDKGKYFKKHPEGTRIDDELKDEIISQVKNNNITCKKAEEI
                     ASALGCSLAETGRAIDILNINITKCQLGLFGYGDTKKIVQPVKEVAPELKESIMSALE
                     NERLSCAAVWGIAKKFNMPRMKVCAACEALDIKVKPCQLGAF"
     gene            complement(2723..3154)
                     /locus_tag="PLF77_09330"
     CDS             complement(2723..3154)
                     /locus_tag="PLF77_09330"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPC09095.1"
                     /translation="MVAYQLSIFAENKPGKLARVTGVLAKEKINIRATTIATADTFGV
                     INLIVDDPRRAEKALSDAGMTVHLREVLAVLISDQPGALDSLTQLFQKEGVNVNNAYG
                     FVLEGSKKAVFVVDVDQIRKTEKLLEHEGFKTLDTQSLSSV"
     gene            complement(3178..4815)
                     /locus_tag="PLF77_09335"
     CDS             complement(3178..4815)
                     /locus_tag="PLF77_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPC09096.1"
                     /translation="MPITEILARNAAQYGEEISLVEINPQIKEIKANWKDYELVEPNP
                     EKRYRREMTWREFDDKANRFANFLLDRGLEKGRKVGILLMNCLEWLPIYFGVLKTGAL
                     AVPLNYRYTEDEIKYCLDLAECDVLVFGPEFTERIDAIKDTIPNVKIRLFVGQDCPAF
                     AESYYALTACCSSANPKISLFDGDDAAIYFSSGTTGFPKAILHSHSALMMSCEVEMNH
                     HGQTRTDNFLCIPPLYHTGAKMHWFGSFLAGSRAVLLRGVKPEWIFKAVSEEQITIVW
                     LLVPWAQDILDAVERGDIKLENYKLDQWRLMHIGAQPVPPSLIKRWKKYFPVHEYDTN
                     YGLSESIGPGCVHLGTENIHKVGAIGVAGYKWDVQIVDEKGTPVKQGEVGELCVKGAG
                     VMKCYYRNPEATAEVLKNGWLYTGDMARMDEDGFIYLVDRKKDVIITGGENIYPVQIE
                     DFLRTHDAIKDVAVIGLPDQRLGEIVAAVIEFKPGFAATESEIAEFCNALPRYKRPRR
                     IIFEKVPRNPTGKIEKPKLREKYFGIGSLVEAQTENL"
     gene            complement(5008..5565)
                     /locus_tag="PLF77_09340"
     CDS             complement(5008..5565)
                     /locus_tag="PLF77_09340"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HPC09097.1"
                     /translation="MLSNKIIRKTPFGPVCIIWSVSKDHPRIIQIMLSRPGLSAENRV
                     SQLFPESKISSCAEIDHVASSIRAYLEGEDITFHLNLVDLSPFSRFQQSVLLAQHAIP
                     RGKVSTYGLIAVHVGAPGGARAVGNVMAINPYPLIIPCHRTVLSDCRLGGFQSGITMK
                     KALLTQEGVMFDQNGRVVCRQLHYD"
     gene            complement(5572..5952)
                     /locus_tag="PLF77_09345"
     CDS             complement(5572..5952)
                     /locus_tag="PLF77_09345"
                     /inference="COORDINATES: protein motif:HMM:NF023390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS/SEC14 domain-containing protein"
                     /protein_id="HPC09098.1"
                     /translation="MGNKAKYKISSSQIDGILEIVLTGEVSKEDVETLQNEIFSIIIN
                     TGAEALLVDVRAIKGRFGYLEAYSRVRHYPTDTPRIHTAIVDNAENADFESFHETTAY
                     NAGLSYKWFTDIEKARSWLKDKIK"
     gene            complement(6017..6709)
                     /locus_tag="PLF77_09350"
     CDS             complement(6017..6709)
                     /locus_tag="PLF77_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09099.1"
                     /translation="MKSSFIPAFIAFGLMSYLAVVQQWSLQEFCWCVWLAGLFYSWTC
                     VMTAAIQMMLTARAQKDYYHANIPLMKKIPPALFALGMIPVALLIGFVGLYLYTWLFS
                     FYGLFLSVFAEMPPVDLFGRNGFINSDFFTPVTYLTDQYWPMILATLAANADGLLRAT
                     PWKRIIFPFKQNEILRIHVMTLLMPFLAMMTWEFFRDAYQQLTIILLIGIFYLLPKRR
                     FKQENMANNPLK"
     gene            complement(6754..7152)
                     /locus_tag="PLF77_09355"
     CDS             complement(6754..7152)
                     /locus_tag="PLF77_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09100.1"
                     /translation="MRDEIIVEYNQEGYVHAKHFGKNNYDLSLEVWRRIKALCKEHNC
                     FKILGENHTTEELSTMDAFNHLNILEEVGITFQYRIAWVDQGTPTAKGLEFVETVVVK
                     NRGLVNGKLFADLEEARRWLLGKDNPQRNS"
BASE COUNT         1780 a         1856 c         1522 g         2105 t
ORIGIN      
        1 ttttcttcag cttttctttt tttctcttct tctttcttta ttctggcttc ttccttaact
       61 ctctttgcct cttcctccct caatcttttt tcctcagctt ttttcttctt ttcttcttct
      121 ttctttattc tggcttcttc cttaactctt tttgcctctt cttcttgcaa acgtttttct
      181 tcaacttttc ttttcttctc ttcctctttc tttattctgg cttcttcctt aactcttttt
      241 gcctcctctt ctttcaaacg cttttcttca gctatcttct ttttttcttc ttctttcttt
      301 attgtatcct gttctttact tttttggacc tcatcctctc ttaatttctc tttctcttct
      361 tcgtcaacac cggtttttgc ttccttgatg taattctcga tcttgtaatc gagcccgacg
      421 ttgctgtatt cggcggctat cttttcaaaa cgcgctaggg ctgctttata ttttttgtga
      481 ttgaggtaaa aattcccgat atagaattcg cgttcggcca gcctctgttt cacttctttc
      541 agcatctttt ccgccataat gctgaattta ctctccggat agcgggcaat taacttttcc
      601 cattcttttc tggcactgat tgtttccgtc tgatcccgat ctattgcttc catacgatga
      661 taatgacaca ttccggtctg atagacggca taaggaacat tttcattgat gggatggaga
      721 gaaataaaat cgctgtaggc atcttttgcc tcttcataat ccttattgct gtatagcgaa
      781 tcggcaatgc ctatttccgc cagtactgac agttcgtgca ggggatattc ttccttgaca
      841 cgtgaaaaat attcccgtgc ctttttgtaa ttcccatcct tatattcctc ggcagccgtt
      901 gcatagagtc cttccgggct agcctgggga aggtcgcgtt tgccgaaaag gctgaaagac
      961 gaacaacctc cgagaattag tgataataac aaaagcgtca aaacactttt taatggcgct
     1021 gtttttacga atttctttgc atgcatcaac gttaacctct cggactgtat tttcggctta
     1081 caatgctttt ttgattaaat cttcaagtct ggctttcggc accaggccga cgatcttttc
     1141 cagaacttta ccctctttaa agagaatcag cgtgggaatg ctgagaacgc cgaaatgcga
     1201 cgccattttc tgattatcat cgacgttcag cttcattatt ttcacactgt ctttatactg
     1261 tccggctatt tcctcaacaa tcggaccgat ggctttacag ggaccgcacc acggtgccca
     1321 gaaatcaaca agcacgggtt tgtcgctttt caaaacttca ttttcaaagt tactgtcggt
     1381 ggcagcagcc agtagttcac tgctcattat aaagccctcc ttagattttt agagtgaatc
     1441 tttatgtcac aataagcccc ataaagtcaa atgcaatctg gcaacagatc cgcaacgacc
     1501 tttgacggtt tcccgttaat ttgctatata atcgggctca aaggagaatc tatgggcatg
     1561 ctgggtatta tttctacaag gctggttctg gaacaaagtg aattgctgaa aaaaagttcc
     1621 gctaagccga tggaaaacgg gttcggtaaa acgactgtgt tcacgacgga taaaacagcg
     1681 ctgattctga gacacggcat ggatccggac aactatattt tacctcattt gatcaatcat
     1741 ccggccaatc ttcaggcgct gaaagatatc ggggtttccg aaattgtaag catcaattcc
     1801 accggatcgc taaaaaaaga aatcaaaccg gggatgatcg tcgttccgga tgattttctg
     1861 actctgactc cctcgccgac cattcacgtg aacagacccg tccacattat cccgtcatta
     1921 aatgaaggca ttaggaaaaa gcttatccga gccgctttga aaaatgattt cgaggtggtg
     1981 cagcaaggaa cctactggca gacacagggc cctcggctgg aaacaaaagc cgaaatcagc
     2041 acgatgaccc gctttgccga cattgtcggc atgaccatgg ccaatgaagc tgtcattgct
     2101 caggaaatgg acattcccta tgcctgcgcc tgttccgtcg ataacttcgg aaacggcctg
     2161 atggctgaac ctttaagcat ggaagaaatc atcagccaca cccgcaaaaa cgcacagcgg
     2221 atgattcaac tgctggaccg ttacctgcaa atgttttttt aaatcagaaa gcacccagtt
     2281 gacagggctt aaccttgatg tccagcgctt cgcacgcggc gcataccttc atccgcggca
     2341 tattaaattt cttcgcaatg ccccagacgg ccgcgcagga cagcctttca ttttccagag
     2401 cggacatgat gctttctttc agctccggcg ccacctcctt caccggttga acgatttttt
     2461 tggtatcacc gtaaccgaaa agtcccagtt ggcatttggt aatattaata ttcagaatat
     2521 ctatcgctct gcccgtttct gccagactgc atcccagtgc cgaggctatc tcttccgcct
     2581 ttttgcaggt gatattatta tttttaacct gactgatgat ctcatctttc aattcatcat
     2641 cgattcgggt cccttccgga tgttttttaa aatatttgcc tttatcctcg tgcgtcatgt
     2701 tgcctcctcc ttgttgaatt tttcatacag aagaaagtga ctgagtgtcc agtgtcttaa
     2761 aaccttcatg ctccaaaagt ttctccgttt tccgtatctg atcgacatca acgacaaata
     2821 cggctttctt cgacccctcc agcacaaagc cgtaggcatt attgacattg accccttcct
     2881 tttgaaatag ttgagtcaga ctgtccagcg cgccgggttg atcggaaatc aaaaccgcca
     2941 acacttcccg caaatgcacc gtcatgcccg cgtcggatag tgctttctcg gcacgccggg
     3001 gatcgtcgac gatcaggttg atcacgccaa acgtatccgc cgttgctatg gttgtggcgc
     3061 ggatattgat tttttctttt gccagaacac cggttacccg ggccagtttc ccgggtttgt
     3121 tttccgcgaa aatagaaagc tgataagcaa ccattccctt ctctccttcc tgttgagtta
     3181 gaggttctcc gtctgcgcct ccacaaggct tccaattccg aaatactttt cacggagttt
     3241 cggcttttcg atttttcccg tgggattgcg aggcaccttt tcaaaaataa tcctgcgcgg
     3301 acgcttatag cgcggcaatg cattacagaa ttccgcaatt tcggattctg tcgcagcaaa
     3361 acccggtttg aattcaatga ccgccgcaac gatttccccc aagcgctgat cgggaagtcc
     3421 aatgaccgcg acgtccttaa tggcgtcatg ggtgcgcagg aaatcctcga tctgcacggg
     3481 ataaatattt tcgccgccgg taataatgac atcctttttg cggtccacca gataaatgaa
     3541 tccgtcttca tccattctcg ccatatctcc ggtgtaaagc cagccgtttt tgagaacttc
     3601 agcggtggct tccggatttc tgtaataaca tttcatcaca cccgcgcctt tgacgcaaag
     3661 ttcacccact tcaccctgct tcaccggtgt gcctttttca tcaacgatct gcacgtccca
     3721 tttataaccg gctacgccga tggcgccgac cttatggata ttttcagtgc cgagatggac
     3781 acaccccggc ccgatggatt ccgaaaggcc gtaattggtg tcgtattcat gtacgggaaa
     3841 atattttttc cagcgtttaa tcaggctggg cggaaccggc tgtgcgccga tgtgcatcag
     3901 ccgccactga tcgagcttgt aattttccag cttgatgtca ccgcgttcga cggcgtccag
     3961 aatatcctgc gcccacggaa ccagcagcca cacgatggta atctgttctt cggaaaccgc
     4021 cttgaaaatc cattccggct tcacaccgcg cagcagaacg gcgcggcttc cggccaggaa
     4081 gctgccgaac cagtgcattt ttgcgcccgt atgataaaga ggcgggatgc agagaaaatt
     4141 gtctgtgcgc gtctggccat gatgattcat ttccacttcg cacgacatca tcagggcgct
     4201 gtgcgaatga agaatggcct ttgggaaacc ggtcgtgcct gacgagaaat aaatggccgc
     4261 atcgtcgccg tcgaaaaggc tgattttcgg atttgccgag gaacagcagg cggtcagcgc
     4321 ataataactt tcagcaaacg cgggacaatc ctgacctaca aacagccgga tcttgacgtt
     4381 cggaatcgta tccttgatcg catcgatcct ttcagtaaat tccggtccga agaccaacac
     4441 atcgcattcg gccagatcca ggcagtattt gatttcatcc tcggtataac ggtagttgag
     4501 cggcacggcc agcgctccgg ttttcaaaac accgaaataa atcggcagcc actccagaca
     4561 gttcatcagc agaatcccga ccttccggcc cttctccaac cctctgtcca gcagaaaatt
     4621 ggcaaagcgg ttggccttat cgtcgaattc gcgccaggtc atttcccgtc tgtatcgttt
     4681 ttcaggattg ggctcgacaa gctcatagtc cttccagttt gccttgattt ctttgatttg
     4741 cggattaatt tcaacaagac ttatctcttc gccgtactga gcggcgttgc gggcaagaat
     4801 ttctgtaatg ggcatttctg attccttcta atctttactg ttttgcaggt tctttatagg
     4861 cgacttttat cctgtcagtt catgatcaac acgcttatca ggaacctgtt aaacgtaaaa
     4921 tacgatacga atttcaggaa tgtatgtcaa gaatgttttg gacaaaataa atgaaacaaa
     4981 agcgctattt ctgatggctg cccctcttta atcgtaatgc aattgacgac aaaccactct
     5041 tccattctga tcaaacatca cgccttcctg tgtcaataaa gcctttttca tcgtaatacc
     5101 gctttgaaaa ccacccaatc gacaatcgga aaggacggtt cggtgacaag gaatgattaa
     5161 aggatatgga ttgattgcca tcacattgcc caccgcccgc gctccccccg gcgctcccac
     5221 atggacggca atgagtccgt acgtgctgac cttgcctcgc ggaatcgcat gctgcgccag
     5281 caaaaccgac tgctggaatc ttgagaaagg cgacaaatca acaagattca gatgaaacgt
     5341 tatatcctcc ccctccagat aggctctgat ggaagaagcg acgtgatcga tttcggcgca
     5401 ggacgatatt tttgattcag ggaaaagttg cgatacgcga ttttcagccg aaaggcccgg
     5461 acgtgaaagc ataatttgaa taatgcgcgg atgatctttg gacaccgacc agataatgca
     5521 gacaggcccg aaaggagttt ttctgataat tttgttactt aacataatag tttattttat
     5581 tttgtctttg agccaggacc tggctttttc aatatcggta aaccatttat aagaaagacc
     5641 ggcattgtat gccgtggttt catgaaaact ttcgaaatca gcattttcgg cattgtctac
     5701 aatcgccgta tgtattcttg gcgtatcggt tggatagtgt ctgaccctgg aataagcctc
     5761 cagatagcca aatcgtccct ttatcgctct aacatccacc agcaaagctt cagcgcctgt
     5821 gtttatgatg atggagaaga tttcattttg taacgtttca acatcctcct tgctcacttc
     5881 gcctgtcaga actatttcca gtattccgtc tatttgcgat gatgaaattt tatatttcgc
     5941 tttatttccc attacagaac tccttaaatt aaatttaata tccggaatcg acttgcagac
     6001 gtcactggcg tttcaattat ttcagtggat tatttgccat attttcttgc ttaaacctcc
     6061 ttttcggaag caggtagaaa atacctatga gcaaaataat tgtcaactgt tgataagcgt
     6121 ccctgaaaaa ttcccaggtc atcatcgcca gaaaaggcat cagcagagtc atcacatgga
     6181 tgcgcaggat ttcattttgc ttgaagggga atatgatccg tttccagggg gttgcgcgca
     6241 gcaggccatc tgcatttgcg gcaagcgtcg ctagaatcat cggccagtac tgatctgtca
     6301 ggtaagtgac cggcgtgaaa aaatccgaat tgataaaacc gttcctgcca aaaagatcga
     6361 ctggcggcat ttcggcaaag acggaaagaa ataatccgta aaaagaaaac agccaggtat
     6421 aaagataaag gccgacaaat ccgatcaaca atgccacggg aatcattccc agggcaaaca
     6481 acgccggcgg gattttcttc atcaggggaa tattcgcgtg gtaataatct ttttgtgccc
     6541 gtgcagtcag catcatctga atcgcagccg tcatcacgca tgtccacgaa taaaaaagtc
     6601 cggccagcca cacgcaccag cagaattcct gcaatgacca ttgctggaca accgccagat
     6661 aggacatcaa gccgaaggca atgaaggctg gaatgaagga tgatttcatg aaggcatgcg
     6721 agtttgattt cagcggcgcc gccaaaatgc ggctcacgaa ttgcgctgcg gattgtcttt
     6781 gcccaacagc cagcgcctgg cctcttcaag gtcagcgaat aatttcccgt ttaccagacc
     6841 gcggtttttc acgacaaccg tttccacgaa ttcgagtccc ttggcagtcg gcgtcccctg
     6901 atccacccac gctatgcggt actgaaaggt tattcccact tcttccagga tattgaggtg
     6961 attgaaagca tccattgtgg agagttcttc cgtggtatga ttctctccca gaatcttgaa
     7021 gcagttatgt tccttgcata gcgccttgat acgcctccat acctcaagcg agaggtcata
     7081 attgttttta ccgaaatgct ttgcatggac gtagccttcc tgattatatt ccacgatgat
     7141 ctcgtcgcgc atcgtacatc ctccctcaac attcagaatg aatgtgactg aattcatttc
     7201 tctgtgttgc acatggaaca caggcttccg cggtaccgat caaaccaatc caaaaaccac
     7261 agg
//
LOCUS       DAOOLB010000090         7229 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_12227_length_7229_cov_45.156259, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000090 DAOOLB010000000
VERSION     DAOOLB010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7229
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12227_length_7229_cov_45.156259"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLF77_09360"
     CDS             complement(<1..534)
                     /locus_tag="PLF77_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPC09101.1"
                     /translation="MKAITLSSCIIGLVAGTFGGLIGIGGGIIMVPLMVEILKLPQHK
                     AHGTSLVALVFTGISGSITYALNGSIDIKAAALLALTAIMTASLGARYAGSLTEQKLK
                     KAFGAFLLFCAALLFLKLYLPNVVVSDDAKPVIYLTTGALTGFLSGMMGVGGGTIMIP
                     VMVILAGFTQHVAQGTSL"
     gene            complement(512..>1192)
                     /locus_tag="PLF77_09365"
     CDS             complement(512..>1192)
                     /locus_tag="PLF77_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC09102.1"
                     /translation="FIFQQLKSPFPLLDIRLFLDNKVFAFSNLAALINYSATFVINFL
                     LSLYLQNIKVLTPSEAGSILIAAPIMQALISPFAGRLSDRFEPQIIASIGMSITVAGL
                     VLLIFLNGSTGIHYVLFCLVLLGIGFALFSSPNVNATMSSVENKFYGIASATLGTMRL
                     TGQMFSMGITMLIFAVILGNHPISAANHHLFLKSTKLIFSILSILCCAGIFASLARGK
                     MHEGNHSL"
     assembly_gap    1193..1292
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1293..1759)
                     /locus_tag="PLF77_09370"
     CDS             complement(<1293..1759)
                     /locus_tag="PLF77_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC09103.1"
                     /translation="MKINANKNHLYVLLVTTLSSFLTPFMGSAINVALPAIAEELSMT
                     ALTLSWVATSFILAASISLIPLGRLADIYGRRRIFIYGALIFTVASAFCIWSPTQAFL
                     IAVRALQGIGGAMIFSTGTAMLISAYPPSDRGKILGINITAVYVGLTVGPFIGG"
     gene            complement(1783..3246)
                     /locus_tag="PLF77_09375"
     CDS             complement(1783..3246)
                     /locus_tag="PLF77_09375"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HPC09104.1"
                     /translation="MKILLAGHNLDYDIIRTLKDKSGTKQDLTPETISAAYARISRNP
                     KPVNELRAIAREEVDKARQSNRKIVFDMGHNSVAEHAVFNIDVIGVSRFLVEEIEKFR
                     LCSFTEKSQRYVLFDKDFVVPEEIRQADLAGLFISTVEAQNDFYHEMYENLRPYIFDK
                     HKTLASDPANRSMLEGWAKEDARYAISMATETQLGMTVNARNLELMLRRLAALPLAEA
                     QEYSAKLYAATKEIAPSLIRYTKATDYDKFTRRNLNRFSDALIKKSQSKVKNAADVQL
                     MSASAAADNRAAAALLFASSDANYAECLKTADKLTAQKKRSLFKTAFANLQIHDAVLR
                     EMETVDLQFELIASSSCFAQLKRHRMSTIIAQEYNPHLGVTVPPSIKAIGRQKDFLDL
                     MHRTQSTYEKIREKAPLAAVYVLTNAHRKRVLMKLNAREMYHLARLRADAHAQWDIHN
                     LTGKMLKQAKKVMPLTLMMACGKDHFPSLLNKTYSCT"
     gene            complement(3256..>3615)
                     /locus_tag="PLF77_09380"
     CDS             complement(3256..>3615)
                     /locus_tag="PLF77_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPC09105.1"
                     /translation="FVPLVEEGWFDHPATKLIAQEYLKQVIAEQVDTLVLGCTHYPLL
                     KPLLQDIMGKSVTLIDSAEAMAGIAADLIDQQAIGNQNHAVPEYTFYVSDLPYRFQTI
                     GERFLGRTLGKIELVSF"
     assembly_gap    3616..3715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3716..3976)
                     /locus_tag="PLF77_09385"
     CDS             complement(<3716..3976)
                     /locus_tag="PLF77_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HPC09106.1"
                     /translation="MNTKANRAIGVFDSGIGGLTVVRALMERLPFENIIYFGDTARVP
                     YGVKSVETINRYAAQITEFLIQKDVKMLIVACNTMAAVAHQTI"
     gene            complement(4214..4855)
                     /locus_tag="PLF77_09390"
     CDS             complement(4214..4855)
                     /locus_tag="PLF77_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09107.1"
                     /translation="MLKISRHIHEKRFPQILTLFFTLILVCLTMVWSADPAYSNDTQD
                     KLYSLNLYYGEISKNHADDFILDTGTLRLTDCRLAAVTLARRLYAYKDLANLEVEGQI
                     VKHFYDIQDHWEFNAIVTARWNAFFWDKYVDTSIAFGLGPSYATEKPEMEAILDRETS
                     QWLAYWMVELEMGLPAHPGISFITRLHHRSDAFRIVSPGGASTAPVVGLKIRF"
     assembly_gap    5024..5123
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5124..5880)
                     /locus_tag="PLF77_09395"
     CDS             complement(<5124..5880)
                     /locus_tag="PLF77_09395"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC09108.1"
                     /translation="MASFKDIFNAAEQGNPAAQYNLGLIYRKGEAVPQDDAEAVGWFL
                     KAAQQNHAKAQLNLAWMYQEGKGVSQDDVKAAKWYLKAAEQGNAQAQFNLALMYSKGE
                     GVALDHIEAAKWFLESAEQGIANAQYNLGIMYQDGLGVQKDSTEAVKWFNKAAKQGHT
                     DAQAKLDAMRKALQDEQREAARAVKESPRILPSNAADASSIPDEFPDEKKEALSQDHA
                     KQILVSQYLKAAEQGLADAQYHLGVIYEEGSGVP"
     gene            complement(6042..>7229)
                     /locus_tag="PLF77_09400"
     CDS             complement(6042..>7229)
                     /locus_tag="PLF77_09400"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HPC09109.1"
                     /translation="YDEKAIRDNARAFINAFNWNEGFKEFFAVKAAPNPYLMKILHKE
                     GYGADCSSLAELMMAQKTGITGEEIMFSSNDTPVEDFLLAKKLKAIINLDDISHIAFL
                     EKSAGLPALICFRYNPGKLKKGNHIIGHPEEAKYGFTREQLFEGYKICRDKGVRRFGI
                     HTMVASNELDPYYFVETAEILFNLIVELSQKLKIKFEFVNIGGGVGIPYRPEQKSIDV
                     NVMSRGIRKKYNDIIVANGLAPLKLFTECGRYITGPYGYLVSRVRHIKNTYKKFAGLD
                     SCMANLMRPALYGAYHHITVLGKERKSHNILYDVTGSLCENNDKFAIDRKLPKLEQGD
                     ILVIHDAGAHGFAMGFNYNGKLRSAELLLRANGKVVQIRRAETIKDYFATLDFNGLKD
                     FNA"
BASE COUNT         1733 a         1620 c         1772 g         1804 t
ORIGIN      
        1 aagcgatgtt ccctgtgcca catgctgggt aaagccggca agaatgacca tcacgggaat
       61 cataatcgtg ccgccgccga cgcccatcat gccggaaaga aatccggtca acgctcctgt
      121 tgtcagataa ataaccggtt ttgcatcatc gcttacgacg acattcggca agtatagttt
      181 taaaaataag agtgctgcac agaaaagcag aaaggccccg aaagcttttt ttaatttttg
      241 ttccgtaagg gaaccggcgt aacgagcgcc cagggatgcc gtcatgattg ccgtaagcgc
      301 cagcagtgcc gccgctttga tgtctatcga tccgttcagt gcataggtta ttgatccgct
      361 gatgcctgtg aaaaccaggg ccacaagact tgtgccgtga gccttgtgct ggggcagctt
      421 taatatctcg accatcagcg gcaccataat gataccgccg ccgatgccga tcagaccgcc
      481 aaacgtgccg gcgaccagtc cgataataca actagagaga gtgattgcct tcatgcattt
      541 tccctctggc cagtgaagca aagatccccg cgcagcaaag aatactcaag atggaaaaaa
      601 taagtttggt gctttttaaa aagagatgat gattggcggc ggaaatcgga tggttgccca
      661 gaataacggc aaaaatcagc atcgtaatgc ccatgctgaa catttgaccg gtaagcctca
      721 tcgtacccag tgtggccgat gcgataccgt aaaatttatt ttccacagaa ctcatggtgg
      781 cattgacatt gggcgacgaa aagagcgcga acccgatgcc cagtaaaaca agacaaaaca
      841 gaacataatg aatgcccgtg gaaccgttga ggaaaattaa aaggacaaga cccgcgaccg
      901 tgatggacat cccgatggac gcgatgattt gcggttcaaa acggtcggaa agacgtccgg
      961 caaaaggcga aataagcgcc tgcatgatcg gggcggcgat cagaatggag cctgcttcgg
     1021 aaggggtcag aactttgatg ttttgcagat aaagactcag caggaaatta ataacaaacg
     1081 tggcgctgta attgatcagg gcggccagat tggagaaagc aaaaaccttg ttatcgagaa
     1141 acaggcgtat gtccagcaag gggaaggggc ttttgagctg ctgaaaaatg aannnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnccgccgat aaaaggcccg acagtcaaac
     1321 caacataaac ggcggtgatg ttgatgccca gaatcttgcc gcgatcgctt ggcggataag
     1381 ctgaaatcag catggccgtt ccggtgctga aaatcatggc cccgccgatg ccctgcaaag
     1441 ctcggacggc aatgagaaaa gcctgcgtgg gcgaccagat gcaaaaggcg gaagccacgg
     1501 taaaaatcaa agccccgtag ataaaaattc ttcggcggcc gtagatatcg gccagacgtc
     1561 ccagagggat gagcgatatg gatgccgcca gaataaaaga agtggccacc caactgagcg
     1621 tcagagcggt catggacaat tcttcggcga tggcggggag ggcaacgttg atggcggatc
     1681 ccataaatgg tgtcagaaaa gaactcagcg ttgtaaccag cagcacatag agatgatttt
     1741 tattggcgtt gattttcatg gatggatgat aaatacggac aattacgtgc agctatacgt
     1801 tttgttcaag agcgacggga aatgatcctt cccgcaggcc atcatcagcg tcagaggcat
     1861 cacttttttc gcctgtttga gcatttttcc cgtaagattg tgaatgtccc actgggcatg
     1921 ggcatcggca cgcagtcgtg caagatgata catttcccgg gcgttcagtt tcatcaaaac
     1981 ccttttacgg tgggcattgg tcagcacata aacggcagcc agtggtgctt tctctctgat
     2041 tttttcatat gtgctctgcg tacggtgcat caggtccaga aaatcttttt gccggccgat
     2101 ggctttaatg gaaggcggca cggtgacacc cagatgcgga ttgtattcct gtgcaatgat
     2161 agtggacatt cggtgtcgtt taagctgggc aaagcaactg gaactggcga tcagttcaaa
     2221 ctgcagatcc acagtttcca tctcccttaa tacggcatcg tgtatctgca ggttggcaaa
     2281 agcagtttta aacagggatc tttttttctg agcggtcagc ttgtcggccg tcttcaggca
     2341 ttcggcataa ttggcatccg atgatgcaaa cagcaatgcg gcagccgccc ggttatcggc
     2401 agcagccgat gcagacatca attgcacatc ggctgcgttt ttgaccttcg attgcgattt
     2461 ttttattaat gcgtcggaaa accgattcag atttcttctt gtgaatttat cgtagtctgt
     2521 cgccttcgtg taacggatca gagaaggggc gatttccttt gtggcggcat acagctttgc
     2581 gctgtattcc tgcgcttcgg ccaggggcaa tgccgccagc cgtctgagca tgagttccag
     2641 gttgcgggcg ttgacggtca tgcccagttg ggtttcggta gccatggaaa tcgcgtagcg
     2701 ggcgtcttcc ttggcccagc cttccagcat ggatcggttc gccggatcgg acgccagagt
     2761 tttatgttta tcgaaaatgt acggcctcaa gttctcatac atctcatggt aaaaatcatt
     2821 ttgcgcttca acggtagaga tgaacaaacc ggcaagatca gcctgccgga tttcttccgg
     2881 gacgacaaaa tccttatcga aaagaacata gcgctgtgat ttttccgtga aggaacacag
     2941 acggaatttt tcgatttctt caaccaggaa ccgggagacg ccgatcacat cgatattaaa
     3001 aaccgcatgc tcggccacgg agttgtgccc catgtcaaaa acgattttac ggttggattg
     3061 gcgagcctta tccacttcct cgcgggcgat ggcacgcagc tcgttaacgg gtttcggatt
     3121 gcggctgatg cgtgcgtaag ccgccgagat ggtttcaggt gtcaaatcct gttttgttcc
     3181 gcttttgtct ttcagcgtcc ggatgatgtc atagtccaga ttatgaccgg caagaagaat
     3241 tttcatgaaa ttttcctaaa aactgacaag ttcaatcttt cccagcgttc ttcccagaaa
     3301 acgttcgccg atggtttgaa aacgataggg caaatcgctg acataaaacg tgtattccgg
     3361 taccgcatgg ttctgattgc cgatcgcttg ttgatcgatc agatcggcgg cgataccggc
     3421 catggcttct gccgaatcga tcagtgttac ggattttccc ataatgtcct gtaacagggg
     3481 ttttaaaagc gggtaatgcg tgcagccgag aaccagcgtg tccacctgtt cggcgataac
     3541 ctgtttgaga tattcctgag caatgagttt tgtcgccgga tgatcaaacc atccttcctc
     3601 caccagggga acgaannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngatcg
     3721 tttgatgagc cactgcggcc atggtgttgc aggcgacaat cagcattttg acatcttttt
     3781 ggatcagaaa ttcggtaatc tgtgccgcgt agcggttgat ggtttccacc gacttcacac
     3841 catacggcac gcgagcggtg tcgccgaaat agatgatgtt ttcaaaaggc agacgttcca
     3901 tcagggcccg gacgaccgtc agaccgccga tgccggaatc gaaaacgcct atggcacgat
     3961 ttgcttttgt gttcatggta atgtttttac ggaactttta tcttaattgt tttttacacc
     4021 gggccgcaaa aaaaaacaac gggccccttt tcttatatct tctcgaaaga aatctcatca
     4081 ttgcggatgt ttgccgctgg aaatggctta tcataactca tcaaaacata gcaaggtttg
     4141 ttgttgatat tttttgtaaa caaggccgct tccccgtcgg gaagacatcg atgcggattt
     4201 taacagcgaa ttgtcagaac cggattttca atcccacaac gggtgctgtg gaggcgccgc
     4261 ccggggatac aatgcgaaag gcatccgagc ggtgatgaag gcgcgtaatg aaggagatgc
     4321 cgggatgggc gggcagaccc atttccagtt cgaccatcca atacgccagc cattgggatg
     4381 tctcacggtc aagaatcgct tccatttccg gtttctctgt tgcataagag gggcccaggc
     4441 cgaacgcgat gcttgtatcc acatacttgt cccaaaaaaa ggcattccag cgagccgtta
     4501 caatggcgtt aaattcccag tggtcctgga tgtcgtagaa atgctttaca atctgtccct
     4561 caacttccag attggcgaga tctttgtatg catagagacg gcgtgccagc gtgacggctg
     4621 ccaggcggca atcggtcagc cggagtgttc cggtgtccaa aatgaaatca tcggcatgat
     4681 ttttcgatat ttcgccatag tagaggttga gagaataaag tttgtcctgt gtatcgttgg
     4741 aataagccgg atcggccgac cagaccatcg tcagacatac aaggatcagc gtaaaaaaaa
     4801 gtgtgagaat ctgaggaaat cttttttcat ggatgtgtcg cgagattttc aacatagaac
     4861 ccgagaaggc agttattttt tgaaaattat ttaaaagaaa aatcaccgta acccattgtc
     4921 cttgttattg atgtgagagt tcatatgata atcagcctgc cgtgtcaatg aattattcgt
     4981 tggggattcc aggatgaaaa tgaagtttat agagcgtaaa aaannnnnnn nnnnnnnnnn
     5041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnggggaac gccggaacct tcttcgtata taacgcccag
     5161 atgatattgt gcatcggcaa ggccctgttc agccgccttc agatattgac ttacaaggat
     5221 ttgtttggcg tggtcctgtg acagggcttc tttcttctcg tcgggaaact cgtccggtat
     5281 ggatgaagca tcggcggcgt ttgatggaag gatgcgaggg gattccttga ccgcccgggc
     5341 tgcttcccgc tgctcgtcct gcagggcttt gcgcatggca tcgagtttag cctgggcatc
     5401 cgtgtgtccc tgcttggctg ccttattgaa ccatttgaca gcttcggtag aatctttttg
     5461 gacacctaac ccgtcctggt acatgatgcc cagattatat tgcgcattgg cgataccctg
     5521 ttccgccgat tccaaaaacc attttgccgc ttcaatatga tccagtgcaa caccttctcc
     5581 cttgctgtac atgagggcca gattgaattg agcctgtgca ttaccctgct cagccgcttt
     5641 cagataccac ttggcagcct ttacatcgtc ctgtgatact ccttttcctt cctgatacat
     5701 ccaggcaagg ttcagctggg ctttggcatg attttgctgg gcggccttga gaaaccagcc
     5761 gacggcttca gcgtcatcct gcgggacggc ttcgcccttc cggtaaatta accccagatt
     5821 atactgtgcg gcggggttcc cttgttcagc tgcgttaaaa atatctttga atgaagccat
     5881 aatatctaaa gggttttgtg atggttgtag aaaattttaa agaagacatg acggtaacat
     5941 cattaatgtc cgcatgcctc tgtctcaatc cgtctggaag tcgatttatt tgacgcacgt
     6001 tcagatcagt gaaacactga tctgcgtcat tccttttata tctacgcgtt gaaatctttc
     6061 agtccgttaa aatccagcgt tgcgaaataa tcctttatgg tttcagcccg gcggatttgg
     6121 accacttttc catttgctct cagtagcagt tcggctgaac gaagtttccc gttatagttg
     6181 aagcccatgg caaaaccgtg cgcgccggca tcatggatga cgagaatatc gccctgctcc
     6241 agtttgggca gcttgcggtc aatggcgaat ttgtcgttgt tttcacaaag cgatccggtg
     6301 acgtcgtaaa ggatattgtg tgatttgcgc tccttgccca gaacggtgat gtgatgatag
     6361 gcgccgtaaa gcgccgggcg catcaggttg gccatgcagg aatccagtcc ggcaaatttt
     6421 ttataggtgt ttttgatatg ccgcacgcgg gaaacaagat aaccgtaggg tccggtgatg
     6481 tagcggccgc attccgtaaa tagttttaaa ggagccagac cgttggccac gataatatca
     6541 ttgtattttt tcctgatgcc gcgactcatt acgtttacgt cgatgctttt ttgctccggc
     6601 cggtagggta ttcctactcc accgccgata ttgacgaatt cgaatttgat tttcagcttc
     6661 tgtgaaagct caacaatcag gttgaacagt atttccgcgg tttccacaaa ataataaggg
     6721 tcaagttcgt ttgaagcgac catcgtatga atgccgaaac gccgaacgcc cttgtccctg
     6781 catattttat agccttcaaa aagctgttcg cgggtgaaac cgtatttggc ttcttccggg
     6841 tggccgataa tatgattgcc ttttttcagt ttgccgggat tgtaacggaa acaaatcagt
     6901 gcaggcaagc ccgcactttt ttccaaaaag gcaatgtggc tgatatcgtc cagattgatg
     6961 atggccttta attttttggc cagcagaaaa tcttccactg gggtatcgtt tgaggagaac
     7021 atgatttctt cgccggtaat gccggttttc tgtgccatca tcaattcggc cagcgaactg
     7081 caatcggcgc cataaccttc cttgtgaagg attttcatca aatagggatt gggagcagcc
     7141 ttaacggcaa aaaattcttt aaatccttca ttccagttga aggcgtttat gaaagcgcgg
     7201 gcattgtccc ggatggcttt ttcgtcata
//
LOCUS       DAOOLB010000091         7191 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_12332_length_7191_cov_59.041340, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000091 DAOOLB010000000
VERSION     DAOOLB010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12332_length_7191_cov_59.041340"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            122..1009
                     /gene="cmr1"
                     /locus_tag="PLF77_09405"
     CDS             122..1009
                     /gene="cmr1"
                     /locus_tag="PLF77_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR01894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr1"
                     /protein_id="HPC09110.1"
                     /translation="MFNIARFKDIEKREYECEIVTPLFLSGADPKKAELRVPPIKAAM
                     RFWWRALYGSDDIEDMAKRESEIFGSTEQKSILKIQVIDKALNLSQAELDKGKFNIYE
                     YLGYGYRVGRDIKTYFADGRLKIQITFKKADRDQIINALGFLIYYGGLGSKSRNGFGC
                     LHCENLQSPLFNHFNKGSLKTFTALSSQALLFDGFSEKSTWKEALAEVGDAYKNGRFE
                     LKNSRMDRSLIAKPFARDHSRHAKPYFLHVRKTNMNKYKGQILYLPYEYHEERKRQDY
                     FRACQRMNKSLEQHLGGAK"
     gene            1006..3708
                     /gene="cas10"
                     /locus_tag="PLF77_09410"
     CDS             1006..3708
                     /gene="cas10"
                     /locus_tag="PLF77_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HPC09111.1"
                     /translation="MSWKKPDISYWNNKFAAWWHDPIDKAFDIQKHESRAAAYLQIFG
                     LDRPNDEFWKMADAIAAGFERGQVPTYSADEHKNGAVDFTRMPVITHPTSDKAHLKIV
                     LDGKNADSIHVETTEYLKKLIGMEASPTDYAGRFKDNEERFALSRLLYTHLVLRFMLA
                     EKNIGGLGALWHRLPADSRFPDHSIWQHNGLCSAIYSCIEMAGKPDDVGLLVFSITPV
                     QGFIARARKMRDFWTGSVVLSWLAFEGIRWIIENLGADHILYPSLIDQPLVNAYLEKE
                     WSVSNAFKPELWKKQTSEIASLPNKFLFLAPFGQAQEIAAEIKKAIHERWSKLSAMTL
                     EYLAEKKGLVKDEINYLQKMFDRQTSHFWEYQWAAARLVDHGDKEELAGLLDETAFSR
                     QFAYLNAIYPILEKKGYSKENASRGILYSTSHHLTQSALAAEKFRRVTRLNEEPGEKC
                     RMCGEFEVLHGSGWTGEAASEYKQNVDDFWNKFLNDDDFKEKERLCAICFIKRYAPRV
                     LKKDKDHVLNNVFGDADSFPSTTEMALRPYFKRRNIKEAKRMKDIAQNLHENEEDKVR
                     GEKVKDTDKYYAILMMDGDNMGKLINGEMIASQWQGIMHPEITARLMEKQYDSLYRDA
                     WRKIYADATLNKRLVTPAIHAAISEALGDFSIYGVAPIIEKNNGRLIYAGGDDVCAFL
                     PAQNALSAAREIKDYYTSTFRLIAQDRQSQEIKDDWKPQPGKLSVNLGKGEEISISAA
                     ILICHHKESLTQMIASAQSLLKSEAKDKAGRNACAVEYRKRHGGSRVFTRKWDDVGWA
                     AFENVQRITGGGERELSHSLLYRLETLRPGIEAIIGSNVNVEENLNSFIAKQIERSGA
                     KLSRPPEEVARWVTQIVWDQKKFSPEGLLIAGFLSGEVENDELV"
     gene            3692..4642
                     /locus_tag="PLF77_09415"
     CDS             3692..4642
                     /locus_tag="PLF77_09415"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HPC09112.1"
                     /translation="MMNWYDFTPQDTLFFRGAEPAVMGESHAASMIFPPPAHTIAGAM
                     RTAVLAQHGIDYKQYNAGGVPPEHDAVISIVGKSGDESPFSIVGPFFRRDGTIWVPCP
                     CIWFSAKKEKASRKRKIAISAPIGKTALLKTAAMENLYWAKGRELESLGGSWVCLDEL
                     CSRSEEKTILENGDFFTSEIHTGIALDVQNSRRTARKSHLYSFVHARLHEGVSLAFGV
                     TQKLPLPERGILKLGAEQRFGEYRQLRKMELPKGNSKLFMTLSTLPADEQANQHCIAS
                     GRPHYYGGWDLHRRFHKPMKGYFPAGSVFQKQINDNCIEL"
     gene            4653..5555
                     /gene="cmr4"
                     /locus_tag="PLF77_09420"
     CDS             4653..5555
                     /gene="cmr4"
                     /locus_tag="PLF77_09420"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HPC09113.1"
                     /translation="MLKRDVFSICTFYAVSPIHSGSGASFAAVDLPIQRERHTHWPHV
                     QASGVKGAMRAHYRDFAEKKSLINFLFGYDKADKDHHVEYFKNDEQKQKVDDNFPGAV
                     SFSDAKLLAFPIRSNVAPFVWVTCPAVLKRLSNDLVFSGLQPINDIEKVPAKDEVAFC
                     LSGGISGNVILEDMVVNVQEGDIPNPIPKEFPDLGKFLLVSDEVFKYAVESCTEIQTQ
                     IKINSETGTAEPGALRYQELLPADTVLYSVVYYSHAVFDNTLQSKTIRGHVEDVIKDF
                     IQIGGDETLGRGICKINWIAGGAQ"
     gene            5552..5956
                     /gene="cmr5"
                     /locus_tag="PLF77_09425"
     CDS             5552..5956
                     /gene="cmr5"
                     /locus_tag="PLF77_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HPC09114.1"
                     /translation="MRTMAQKRAKYALEKVLQSMQRISDKDKFSSFTAGAPAMILQNG
                     FGQTLAFWLTKGTKNGRIDSNDKHIILFDIVKDWLSLQDGDTRNNFAIAGDRVDLMRQ
                     ISAMNQNQFLSVQIETLALLEWVKRFANADLE"
     gene            5920..7125
                     /gene="cmr6"
                     /locus_tag="PLF77_09430"
     CDS             5920..7125
                     /gene="cmr6"
                     /locus_tag="PLF77_09430"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr6"
                     /protein_id="HPC09115.1"
                     /translation="MGEAICKCRLGIKEIVMPQEQVIYPFPDTVKEQAKDMTKGNFGL
                     WYNKFIPVKTHEDKKDAFKTCDASGKVPEVVEFYEARYKLMQKETAVMLKRLLGKKHQ
                     DQSGYCGSFSESDYKVITIRASLKTPLITGIGELHPHEVSMVFDHNLGIPYIPAAGVK
                     GIVRFAHTLSIFLDETGKVKEEYQNQDSIEESITDIPDIFGGIKAKGKEKDVLRGRAV
                     FLDAYPENVPDLHIDIMNPHYADYYGDPRKQTPPADYLSPNPLKFLTVAPGAVYVFRA
                     IARKESDIPRKVKEALSTALTEEGVGAKTALGYGRFTIDEKASPATAAQKCITKKIEQ
                     TPLERCCTPFKTIKPSEAGKIGPLIDMALKTLTTEADKRAFAQYVKEFLGNDFKKSKA
                     REKLKVFLA"
BASE COUNT         2099 a         1556 c         1820 g         1716 t
ORIGIN      
        1 ggaaagatga agttggatgt atcaaaagcc gaaagccgcc gcggcaacgt tgccggaata
       61 gatagaacag atgatgtttt gatataagcg gattcagaaa agaacaggag ggaaattttt
      121 tatgttcaac attgcacgat ttaaagacat cgaaaagcgg gaatatgaat gcgagatcgt
      181 cacgccgctt tttctgagtg gggcggaccc gaaaaaggcc gagcttcgcg tgccgccgat
      241 caaagcggcc atgcggtttt ggtggcgggc gctgtatggc agcgacgaca ttgaggatat
      301 ggcaaagaga gaatcagaaa tctttggttc aacagaacaa aaatcgatct taaaaataca
      361 agtgatagat aaagcgttga atttgtcgca ggcggaattg gataaaggaa aatttaacat
      421 ttatgaatac cttggttatg gatatcgcgt gggaagggac attaaaacgt attttgcaga
      481 tggtcgactc aaaattcaga ttactttcaa aaaagcggat cgcgaccaga taataaatgc
      541 acttggcttt cttatttatt atggtggttt gggttccaag tcccgcaatg gttttggttg
      601 tctgcattgt gagaatttac agtcccctct cttcaatcat tttaataaag gttcgctgaa
      661 aacttttaca gcattatcct cacaggcttt gttattcgat ggatttagtg aaaaatcaac
      721 atggaaagaa gcgcttgctg aagtgggcga tgcctataaa aatggacgtt tcgaactaaa
      781 aaacagtcgc atggatagat ctctcattgc aaaacctttt gctcgcgatc attcaagaca
      841 tgcaaagcct tattttcttc atgtaagaaa gaccaatatg aacaaataca aggggcaaat
      901 tctttattta ccttatgagt atcatgagga aagaaaacgc caagactatt tccgagcgtg
      961 tcagagaatg aataaatcac tagaacaaca tctgggaggc gccaaatgag ctggaaaaaa
     1021 cccgacatct catactggaa caacaagttt gcggcctggt ggcatgatcc catcgataaa
     1081 gcttttgata tccagaaaca cgaatcgcgc gcagcggcct atttgcagat atttggcctg
     1141 gacagaccca atgatgaatt ctggaaaatg gcggacgcca ttgcagcagg gtttgaacgc
     1201 ggccaggttc ccacctatag cgctgatgag cataaaaacg gcgcggtgga tttcacccgg
     1261 atgcccgtga ttactcatcc tacgtcggac aaagcccatt taaaaattgt gcttgacggt
     1321 aaaaacgcag acagcattca tgtcgaaact actgaatatt tgaaaaaact gatcggcatg
     1381 gaggccagtc caacagatta tgccgggcgc tttaaggata acgaagaacg gttcgcctta
     1441 tcccggctgc tttatacaca tcttgttttg cgtttcatgc tggcggaaaa aaacatcggg
     1501 gggctgggcg cgctatggca ccggctgccc gcggattccc gttttcccga tcattccatc
     1561 tggcagcaca acggcctttg ctcggccata tactcctgca tcgaaatggc cggaaaacct
     1621 gatgacgtgg gattgcttgt gttttccatc acacccgtcc agggttttat cgcccgtgcg
     1681 cggaaaatgc gtgatttctg gacgggctct gttgttcttt cctggctggc atttgaggga
     1741 atccgctgga ttattgagaa tctgggcgcc gatcatatct tgtatccttc gcttatcgat
     1801 cagccactgg tcaatgccta tctggaaaaa gaatggtctg tgtcaaatgc gttcaagcct
     1861 gagttatgga aaaagcaaac cagtgaaatt gccagcttgc ccaacaagtt tttgttcttg
     1921 gcgcctttcg gccaggcgca agaaatagcg gctgagatca aaaaggcaat ccatgaacga
     1981 tggagtaaac tttccgccat gacgcttgag tatctggccg aaaagaaagg cctggtcaaa
     2041 gacgaaataa attacctgca aaaaatgttt gaccggcaaa ccagtcattt ttgggaatac
     2101 cagtgggcgg cagcgcggct cgttgaccac ggggacaaag aagaattggc cggactactc
     2161 gatgaaacag ccttttcaag acagttcgct tatctcaacg ccatttatcc gattttggaa
     2221 aagaaaggct attcaaagga aaatgccagt cgcggcattt tatattcaac gagtcaccac
     2281 ctgacgcaat ccgcccttgc cgcggagaaa ttccggagag taacgcgtct taatgaagaa
     2341 ccgggtgaaa aatgccggat gtgcggcgaa tttgaagttt tgcatggcag cggctggaca
     2401 ggcgaagcgg catccgaata caaacaaaat gtggacgact tttggaataa atttctcaat
     2461 gacgatgact tcaaagaaaa ggaacgcctc tgtgccatat gtttcattaa acgctatgcg
     2521 cccagagttt tgaaaaaaga caaagatcac gttttgaata atgtttttgg tgacgcggat
     2581 tcctttccat ccacaaccga aatggcgctg cggccttatt tcaaaaggcg aaacataaaa
     2641 gaagccaagc gaatgaaaga tattgcgcaa aaccttcatg aaaacgaaga agataaggta
     2701 aggggcgaaa aggtcaaaga cacggacaag tattacgcca tactgatgat ggacggcgac
     2761 aatatgggga aactgattaa cggtgaaatg atcgcatcgc agtggcaagg gattatgcac
     2821 ccggagatca cagcgcgctt gatggaaaag cagtatgatt ctttatacag agatgcctgg
     2881 cgaaagattt atgccgatgc gaccctgaat aaacgcctgg tgacgcccgc gattcacgcg
     2941 gcgatatccg aggctctggg cgatttttcg atctatggcg ttgcgccgat tattgagaag
     3001 aacaacggcc gtctgattta tgcgggcggc gacgacgttt gcgccttcct gcccgcgcaa
     3061 aacgcattat ccgccgcgcg ggaaattaaa gattattata cctctacgtt tcgcctaatc
     3121 gctcaggaca ggcaatcgca agagattaaa gatgactgga agcctcagcc gggcaaactc
     3181 tctgtaaatt tgggaaaggg tgaggaaata tccatatcgg ccgcaattct gatctgccat
     3241 cacaaggaaa gtctgacaca gatgatcgca agcgctcaga gcctgctgaa aagcgaagcc
     3301 aaagacaaag cggggcgaaa cgcctgcgcg gtggaatatc gaaaaaggca cggcggttcc
     3361 cgtgtcttca ctcgaaaatg ggatgatgtc ggatgggcag cttttgagaa tgtccagcgg
     3421 attacaggcg gcggagagcg ggaattgtcc cattctctgc tgtatcgcct tgaaacgctg
     3481 cgtcccggca tagaagcgat tatcggaagc aacgtaaatg ttgaagagaa tctgaattct
     3541 tttatcgcga aacaaattga acggtcaggc gctaaattat ccaggccgcc ggaagaagtt
     3601 gccagatggg taacgcaaat tgtttgggac cagaaaaaat tcagtcctga aggtttgctg
     3661 attgcgggat ttttgagcgg ggaggtcgaa aatgatgaac tggtatgatt ttacgccaca
     3721 agacacgtta ttttttcgtg gagccgaacc ggccgttatg ggcgaaagcc acgcggcttc
     3781 catgatcttc ccgccgcctg cgcataccat tgccggcgca atgcgcacgg cggttctggc
     3841 gcagcatggc atagattata agcaatataa tgcagggggg gtgcctcccg agcatgatgc
     3901 cgtcatcagt attgtaggga agtctggaga tgaaagcccc tttagtattg tggggccgtt
     3961 ttttcgtcgc gacggaacaa tctgggttcc ctgtccctgt atctggtttt ccgctaaaaa
     4021 agaaaaggcg tcaaggaagc ggaaaatagc gatcagcgcg ccgataggaa aaaccgcgct
     4081 gttaaaaacg gcggcaatgg aaaatttata ctgggcgaag gggcgagagc ttgaatcatt
     4141 gggcggctct tgggtttgtc ttgatgaact gtgcagccgg tctgaagaaa aaacgatcct
     4201 tgaaaacggc gatttcttca ccagtgaaat ccacacgggc attgctctgg atgtccaaaa
     4261 cagccgccgc accgcgagaa agagtcactt gtattcgttt gtccacgcca gattgcatga
     4321 aggtgtgtct ctggcgttcg gcgttacaca aaagttgccg cttcccgaac gcggaatact
     4381 taagctgggc gccgaacagc gtttcggcga atacaggcaa ttgcgtaaaa tggagctacc
     4441 gaaaggcaat tccaagttgt tcatgacgct ttccacactg ccggccgacg agcaggccaa
     4501 tcagcattgt attgcgtcag ggcggcctca ttattacggc ggatgggatc tgcacaggag
     4561 atttcacaaa ccgatgaaag gctactttcc agcgggaagc gtgtttcaaa aacaaattaa
     4621 cgataattgc atagaattat aggaggacta ccatgttgaa aagagacgta ttttccattt
     4681 gcacatttta cgccgtatca cccattcatt caggaagcgg agcatccttt gccgccgttg
     4741 accttccgat tcagcgcgaa cgacacacgc attggccgca tgttcaggcg tcgggggtta
     4801 aaggggccat gcgcgcgcat tatcgagatt ttgcggagaa aaaatcccta attaatttct
     4861 tgttcggtta cgataaagcc gacaaagatc atcacgtgga gtatttcaaa aatgatgaac
     4921 aaaagcagaa agtggacgat aattttccgg gcgccgtatc tttttcagac gcaaaacttc
     4981 tcgcctttcc aattcgctcc aatgtcgcgc cgtttgtttg ggtgacatgc ccggctgttt
     5041 tgaaaagatt gagcaatgac ttggtttttt ccggtctgca accaatcaat gatattgaga
     5101 aagttccggc aaaagacgaa gtcgcgttct gtctatccgg cggcatctcc gggaacgtca
     5161 ttctggaaga catggtcgta aacgtccagg aaggcgacat acccaatccc attcccaaag
     5221 aatttcccga tttgggcaaa ttcctgttgg tttcggatga ggtttttaaa tacgcggttg
     5281 agtcctgcac ggaaatccag acccagatta aaattaattc cgaaaccggc actgcagaac
     5341 ccggcgcatt gcgctaccag gagttgctgc cggccgatac agtcctctac tccgttgttt
     5401 actacagcca cgccgttttc gataataccc tgcaatctaa aaccattcgc ggccatgttg
     5461 aagacgtcat aaaagatttt atccaaatcg gcggcgatga aacattgggc cggggtatct
     5521 gtaaaatcaa ctggattgcc ggaggtgcac aatgagaaca atggcacaaa aaagggctaa
     5581 atatgctctg gaaaaagttc tgcaaagtat gcagcgaata tccgataagg ataaattttc
     5641 atcctttaca gccggagcgc cagcgatgat cctccaaaat ggttttggtc aaacacttgc
     5701 tttctggctt acaaaaggaa caaagaacgg tcgcatagat agtaatgata aacatataat
     5761 tttgtttgat attgttaaag attggctgtc attgcaagat ggagataccc gaaacaactt
     5821 cgcaattgcc ggtgatcggg tggatttgat gcgtcaaatt tccgcgatga atcaaaatca
     5881 atttctttcg gttcaaattg agactttggc tctactcgaa tgggtgaagc gatttgcaaa
     5941 tgcagacttg gaataaagga gattgtcatg ccacaggaac aggttattta tccgtttcca
     6001 gacactgtaa aagaacaagc caaagacatg acgaaaggga acttcggtct ctggtataac
     6061 aaatttattc cggtcaagac acatgaagat aagaaagatg cctttaaaac ctgcgatgcg
     6121 tccggaaaag tgccagaggt cgtcgaattt tatgaggcgc gatacaagct gatgcaaaag
     6181 gaaactgctg tcatgttgaa gaggcttctt ggaaaaaaac atcaggatca gtctggctat
     6241 tgcggaagtt tctctgaaag tgattataag gtaatcacta ttcgggcgtc gctcaagaca
     6301 ccgctcatca cgggaatcgg tgagttgcac ccgcatgagg tgagcatggt ctttgaccac
     6361 aatctcggta tcccctatat tccggcggcg ggagtaaaag gaattgtgcg ctttgcgcat
     6421 acgctgtcga tttttttaga tgaaaccgga aaggttaagg aagaatatca gaatcaagat
     6481 tctattgagg aaagcatcac agatatacct gatatttttg gcggaataaa agcaaaggga
     6541 aaagaaaagg acgtattacg cggacgcgcc gtttttcttg acgcctaccc tgagaatgtt
     6601 ccggaccttc atattgacat tatgaacccg cactatgcag attactatgg cgacccgaga
     6661 aaacagacgc cgccagccga ttatctttcg ccaaatccct tgaaatttct gacagtggcg
     6721 ccgggagcgg tttatgtctt ccgtgcgatt gcccgtaaag aaagcgacat tccacggaaa
     6781 gtcaaagaag ctttgagcac ggcattgacc gaagaaggcg tcggcgcaaa aacggcttta
     6841 ggttatggac gattcaccat cgatgaaaaa gcgtcgcccg caacagcggc tcaaaagtgc
     6901 ataacaaaaa aaatcgagca gacaccgctg gaaagatgct gtacgccgtt taaaacgatt
     6961 aagcccagcg aagcaggaaa aattggcccg ctgattgaca tggcgctgaa aaccttgact
     7021 actgaagcag ataaaagagc atttgctcaa tatgtgaagg aatttttggg aaacgacttt
     7081 aagaaaagca aggcaaggga aaagttaaaa gtattcttgg cataggcatc gttattgaaa
     7141 aaacccgtca aagcccgcgt taccgtacgc aaggcgggga atagctggga a
//
LOCUS       DAOOLB010000092         7073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_12596_length_7073_cov_46.927757, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000092 DAOOLB010000000
VERSION     DAOOLB010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12596_length_7073_cov_46.927757"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..3112
                     /locus_tag="PLF77_09435"
     CDS             53..3112
                     /locus_tag="PLF77_09435"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPC09116.1"
                     /translation="MPLIRKYQVNWSIIFVVALIVTGLSVWESRNLKIDTDILESMPH
                     KDPVLADARLIIKHLPVQDKVFINLEQTSPDRNKLIDTAAAVTDQLNKSGLFIRVGIG
                     DDAENFPELIAHVNDNLPALLSADDLEQKIAPLLTPDNIREAMAQNRQALVQLEGIGR
                     SDMMTKDPLGFSSVILTQMSALLPANKALFYQGQLISEDGTNALIVARIKGSGTDTAV
                     AEKIEKLLEDCRRDLAAKGHPYKLTATGAYRVALDNETTAKSDMQMAVTFTTLGIAFL
                     LILAFPRPLIGLLALLPSSVGAIAALFVCSFLFKSMSIIAVGFGSAIMAFTVDLGIAY
                     LLFLDQPHATCGKDVAREVWSAEFLAVLTTVGSFLLLLISDFKILAQIGVFSALGVAF
                     ALLFVHLVFPRIFPAMPPARKPGNPLLSNAVQKFAAPAKWKLIAAIIFGLAMLFFAKP
                     VFNVDMNSMNSMSEATMNADKKMQEVWGNLSGRCYVFLQASDMTQLREKNDQLAGWLA
                     GDIRDEKLSPPFLPTVLFPSPETAQHNYAAWSAFWNKDRVAELKRNLSAAGKENDFAP
                     DAFEPFWKIINRTTIGSFDIPEKYFDMLGIAKSSAGYTQLSLLASGKNYNAEDFFERL
                     SRSGLAKIFDADLFNKRLGDFLKDLFLEIAIIISIGLVLITFLYFLDWQLTLAAMAPI
                     VFALFATLGTMKIIGHPLDIPGIMLWIIIMGMGDDYSVYYICHYQRNVDEKSLAMHTI
                     KLAIFLAALTTLIGFGVLALADHALLRSIGIVSFLGIIYSLIGVFFILPSLMDKIFAP
                     IAYPADKVVIGSRDHLRRTVLRYRHLPGYPRVFARMKIMMDPMFRELHKYVKNPRRII
                     DIGCGFGVPATWLLEIYPDARVFGIEPDVERVLIASRVIGSRGRVEAGRAPDLPHVEG
                     EADHVLMLDMLHLISDDEVKIVFQRIYDKLTADGTLLIRTTIPSDKPNPWKRWIETSR
                     LKMTGMPNRFRSETEIADFMKQAGFAVQVFASDTRSIEEKWFAGKKY"
     gene            3284..4054
                     /locus_tag="PLF77_09440"
     CDS             3284..4054
                     /locus_tag="PLF77_09440"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001389.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPC09117.1"
                     /translation="MNIIQTIILAAIEGITEFLPVSSTGHMILASSLMNIHDNAFVKS
                     FEIAIQIGAIGAIIMLYYKRFLKGIKIYIKLGIAFIPTAVIGFLAYKTIKMHLFNPLI
                     VSTALIVGGVILILVDKRVVNSKTQYMELEDISYKHAILIGLAQCVSMIPGVSRAAAT
                     IIGGVFNGLNKKQATEFSFLLAVPTMCAATGYDLIKTDAAFSSYEQTLLVIGFAVAFI
                     FAYLAVKIFLKIVENYGFKYFGYYRILIGLVFIIYAVR"
     gene            4226..>4535
                     /locus_tag="PLF77_09445"
     CDS             4226..>4535
                     /locus_tag="PLF77_09445"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HPC09118.1"
                     /translation="MKKSFSVCCGVVLLLMSVVFSGCSGSSGSDSESSPRIATTSLPS
                     CVIASAYSQQISVTGGNGTKMFSITKGSLPSGITLSSSGRIAGTTWAAPGVFQFTVTV
                     E"
     assembly_gap    4536..4635
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4636..5496
                     /locus_tag="PLF77_09450"
     CDS             <4636..5496
                     /locus_tag="PLF77_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HPC09119.1"
                     /translation="AGTTWAAPGVFQFTVTVEDAAGSSTQDLSLAVVADNIAGPHPYE
                     TIRNNPSYYYVSQEAGLCAATCFYMTMKYYGDHLKNIKTGIDDDNCPAEITDRIAYPT
                     ELSATSQIATYIQYMDNSFNNLAGIHLTSLARAAENLLDESGSRALYSEVMIENYSND
                     LTGTEDSISEQKRNIFLNRMVPFLNSGSPVLVHLWRSPVLGIPSSGHYVLVIGYDDAE
                     KAVYFMDPNDQNHKGNQCDCVAVDSSNSDICFIQKVAFDHFIEEYWYKSDDPLVLNAR
                     WDGNWIGFHH"
     gene            5596..6489
                     /locus_tag="PLF77_09455"
     CDS             5596..6489
                     /locus_tag="PLF77_09455"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPC09120.1"
                     /translation="MKKLPYKLLLFFTHRLGLWFFRIISWFIATGYFFLFPVRVANSF
                     EFYRTLFPGKGFFYHLWCAWQQYHKFTDVYIHRFIRFDENDIEYIKEGWEHLDAAVQN
                     KTGAILLMSHIGNWELAAQTLNKRGLPIMLYLGAKHKEQIERIQKEAMAKSGIKIVAT
                     SEDEKSPFALIDGINFLREGGIVSMAGDRLWGDQSHIAVNFLGHEVRLPDTPHLFALM
                     TGAPLMTFFVYQEAVGKYHIRISPGRKVTAAARADRKKAVQNSVQAYADDLAEFARQH
                     PFEWHHFEPFLEKSHSASLRE"
     gene            6756..>7073
                     /locus_tag="PLF77_09460"
     CDS             6756..>7073
                     /locus_tag="PLF77_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09121.1"
                     /translation="MKVNEVPQDNTVEYYEGVKRACYAVDEKGRYVIVSSNGWEAEEL
                     VNGLAVKELAVNLEKTRKAVLAGEKSPLAYHMERRQMIPDILGKTAGIAVFRVKRHLR
                     PEIF"
BASE COUNT         1776 a         1673 c         1718 g         1806 t
ORIGIN      
        1 tttacacaat cagccttaag tatctataac taaatgaaaa ataaaggtgt tcatgccgct
       61 catccgcaaa tatcaggtaa actggtccat tatttttgtc gttgcgctca ttgtcacagg
      121 gctttctgtc tgggaatcca gaaatcttaa aatcgatacg gatattctgg aatccatgcc
      181 gcataaagac ccggtattgg cagacgcgcg tctgattatc aaacacctgc ccgttcagga
      241 taaggtgttc attaatcttg aacagacatc tcccgaccgc aacaagctga ttgacactgc
      301 ggccgcggtc accgaccagc tcaacaagag cggcctgttt attcgggtgg gcatcgggga
      361 cgacgctgaa aactttcccg aacttatcgc tcatgtcaac gataacctgc ccgcactttt
      421 aagtgctgat gatctggaac agaaaattgc gccgctgctg acgccggata acatcagaga
      481 ggccatggcg caaaaccggc aagccctcgt gcagcttgaa ggaatcggcc gcagcgacat
      541 gatgaccaaa gacccgctgg gattttccag tgtcattctc acacagatgt ccgccttgct
      601 gcctgccaac aaggcgctgt tttatcaggg gcaattgatt tccgaagacg gcacaaacgc
      661 gttgatcgtt gccagaatta aaggttcagg aacagacacg gcagtggctg aaaaaatcga
      721 aaaacttctg gaagattgcc ggagagatct agccgcaaaa ggccatccat ataaactgac
      781 tgccacgggc gcttaccgtg tggcgctgga taacgaaacc acagccaaaa gcgatatgca
      841 gatggccgta accttcacaa ccctgggcat cgcttttttg ctcatccttg cgtttccgcg
      901 tccgctcatc ggcctgctgg cgctgctgcc ttcgtccgtc ggcgcgattg ccgctctctt
      961 cgtttgttct tttttattca aatccatgtc gatcatcgct gtcggtttcg gcagcgccat
     1021 tatggccttt accgttgatc tgggcatcgc ctacctgctt tttctggatc agccccatgc
     1081 cacctgcgga aaagatgtgg cgcgggaagt ctggtcggcg gagtttctgg ccgtcctgac
     1141 cacggtcggc tcttttctgc tgctgctcat cagcgatttt aagattctgg cgcaaatcgg
     1201 cgttttttcc gccttgggcg tcgctttcgc tctgctgttt gtccatcttg tttttccaag
     1261 aatatttccg gccatgccgc cggcgagaaa gccgggcaac ccgctgttgt ctaatgctgt
     1321 tcaaaaattt gccgctccgg ccaaatggaa attgattgcc gcgattattt tcggtctagc
     1381 gatgcttttc tttgccaagc cggttttcaa tgtggatatg aattcgatga actccatgag
     1441 cgaagcaacc atgaatgccg acaaaaaaat gcaggaggtg tggggtaatc tttccggccg
     1501 gtgttatgta tttcttcagg catccgatat gacgcaactt cgggaaaaaa acgatcaact
     1561 ggccggttgg ctggctgggg atatccggga tgaaaaatta tctcctcctt ttctgccgac
     1621 cgttttgttt ccctccccgg agacggccca acacaattat gcggcctgga gtgccttctg
     1681 gaataaagac cgcgtggctg aattaaagcg caatctaagc gcagcgggga aggaaaacga
     1741 ttttgcgccc gatgcttttg aacccttctg gaaaatcatc aatcggacaa ccatcggctc
     1801 gttcgatata cctgaaaaat atttcgatat gctggggatt gccaaaagtt ccgcgggtta
     1861 tacgcaactc agtttgctcg cctccggtaa aaattacaac gcggaagact tctttgagcg
     1921 gctttcccga agcggtctgg ctaaaatatt cgacgctgac ttgttcaaca agcggctggg
     1981 cgattttctg aaagatcttt tcctggaaat cgccatcatc atcagcatcg gcctggtgtt
     2041 gattactttt ctatattttc tggactggca gctgacgctt gcagcaatgg cgccgattgt
     2101 cttcgcgctt tttgccacgc tcggcacgat gaaaatcatc ggccatccgc tggatattcc
     2161 cggaattatg ctctggatca tcatcatggg catgggcgac gattattccg tctattacat
     2221 ttgtcattac cagcgtaacg tcgatgaaaa aagtctggcc atgcacacca tcaaactggc
     2281 catatttctg gcggcgctga ccaccttaat cggcttcggc gtgctggcac tggccgacca
     2341 cgcgcttttg cgcagtatcg gcattgtctc ctttctcgga atcatttact ccctgatcgg
     2401 cgtctttttt atcctgccgt ctctgatgga caaaatattt gcgccgattg cataccccgc
     2461 ggacaaagtg gtaatcggtt cccgcgacca tctgcggcgg accgttttgc gctaccggca
     2521 tctacccggg tatccgaggg tctttgctcg catgaaaata atgatggatc cgatgttcag
     2581 ggaactgcat aagtatgtaa aaaatccccg taggattata gatatcggct gtggctttgg
     2641 cgtgcccgcc acctggcttc tggaaattta tccggatgcc cgggtgttcg gtattgaacc
     2701 cgatgttgag cgggttttga tcgcctcgcg cgtcatcggc agccgtggca gggtggaagc
     2761 aggacgggcg ccggatttac cgcatgtcga aggcgaggcg gatcacgttc tcatgctcga
     2821 catgctgcac ctgatttccg atgatgaagt aaaaattgtt tttcagcgga tttacgataa
     2881 actgactgca gacgggacat tgcttatccg cacaaccatt ccgtcggata aacccaatcc
     2941 gtggaaacgc tggatcgaaa ccagtcgatt aaaaatgacc ggcatgccca accggtttcg
     3001 atcagaaacg gaaattgccg atttcatgaa acaggccggc tttgccgtcc aggtcttcgc
     3061 ttccgatacc cggagtattg aagaaaaatg gtttgcgggt aaaaaatatt aggccggcag
     3121 gaaattcccg caaatgtttg aaaaatgcag aaaaacggtt taggaaaaca cgtaaatata
     3181 aaatccttta tgaaaatacg aaaataaaag tagattttta ccggtataaa aaataatacg
     3241 gcacgattcc atctatgtgt cgtaacgaaa attgaggggg aaaatgaata ttattcagac
     3301 aatcattctg gcagcgatag aggggattac cgagtttctg ccggtttcct ccaccgggca
     3361 catgatttta gcctcatcct tgatgaatat tcacgacaat gctttcgtaa agagctttga
     3421 aattgccatt caaatcggag cgatcggagc gatcatcatg ctttactata agcgttttct
     3481 gaaaggcata aaaatataca tcaagctcgg aattgctttt attccaacgg cggtgattgg
     3541 ttttctggcc tataaaacca ttaaaatgca tctgttcaac ccattgatcg tttctacagc
     3601 gctcattgtc ggcggcgtca ttctcatcct cgtagacaaa cgcgtggtga acagtaaaac
     3661 acaatatatg gagttggagg acatctccta caagcacgcc atattgattg gattggcaca
     3721 atgtgtttcc atgattcccg gtgtatcgcg cgccgcggca accattattg gcggcgtttt
     3781 caacggatta aacaagaagc aggccaccga gttttccttt ctgctggcgg ttcccacgat
     3841 gtgtgcagca acggggtacg atttgatcaa aaccgatgcc gccttttcgt cttacgagca
     3901 gacgctgtta gtcatcggtt ttgcggttgc ttttattttt gcttatcttg cggttaagat
     3961 atttttaaag attgttgaaa actatggatt caaatatttt ggatactacc gcattttgat
     4021 aggactggtc ttcattattt acgcggtcag atgagacaag aaacaaggag gctttgctta
     4081 tgggaaagaa gggttagcca ggcagcaaac ccgctgcatg cccgagtcaa agcaagagag
     4141 actatgaaca aacaacaata tcatatatca ttttgtgcat taccggaaaa gttcacggga
     4201 agagatcaga aggcaggata agaaaatgaa aaaaagtttc agcgtatgtt gtggggtggt
     4261 cttattgttg atgagtgttg tgttcagcgg ctgttccggt tcgtccggct ccgattctga
     4321 gtcttcaccc agaattgcca caacctcatt accctcctgc gtcattgctt cggcttacag
     4381 ccagcagatc agcgtaaccg gcggcaacgg cacgaaaatg ttctccatca ccaaagggtc
     4441 attgccctca ggcatcactt tgtcttcttc cggcagaatc gccggcacca catgggcagc
     4501 tccaggggta tttcaattta ccgtgacggt cgaagnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnngccgg caccacatgg gcagctccag gggtatttca atttaccgtg
     4681 acggtcgaag acgccgcagg ctccagcaca caggatcttt ctctggctgt tgtcgcggac
     4741 aacattgccg gaccgcatcc ctatgagacg atccggaaca atccgtcata ctactacgtc
     4801 tcgcaggaag cgggattatg cgcggcaaca tgtttttaca tgaccatgaa atattacggc
     4861 gatcatctga aaaatatcaa aaccggaatt gatgacgaca attgtccggc ggaaatcaca
     4921 gatagaattg cttatccgac ggaactgtca gcaacatcgc aaatagcaac atatattcaa
     4981 tacatggaca actcattcaa caacctggca ggcatccacc tgacttcctt agctcgggcg
     5041 gcagaaaacc ttctggatga atcaggtagc cgggcgttgt acagtgaggt gatgatagaa
     5101 aattacagca atgatctgac cggaacagag gacagcattt ccgaacaaaa aaggaatatt
     5161 tttcttaatc gaatggttcc gtttttgaat agcggctctc cagtgctggt gcacctttgg
     5221 agatcgccgg tgctgggcat tccttcttcc ggtcattatg ttctggtgat cggatatgat
     5281 gatgccgaga aggcggtgta ttttatggat ccaaacgatc aaaatcacaa aggaaatcaa
     5341 tgtgattgcg tagcggtgga cagcagtaat tccgatatat gtttcattca aaaggttgca
     5401 ttcgaccact tcatcgaaga gtactggtat aaatccgacg atcccctggt cctcaatgca
     5461 agatgggatg gaaactggat cggcttccat cattaaaaaa taataagggt cgaaatcttt
     5521 acctggctca cgttggaaga aaaaggactg gataccgggt ccccgatcag gtcgaagatg
     5581 acaagatatt gagttatgaa gaaactacct tataagctat tgctcttttt cacccacagg
     5641 ctggggttgt ggtttttccg aatcatttcc tggtttatcg ccacgggtta tttttttctt
     5701 tttccggtgc gtgtggctaa cagtttcgag ttttatcgca ccttatttcc ggggaagggc
     5761 tttttctacc atctctggtg cgcatggcaa cagtatcata aattcaccga cgtctatatc
     5821 caccgtttca tccgctttga cgaaaacgat atcgagtata tcaaagaagg atgggaacat
     5881 cttgatgcgg ccgtgcagaa caaaacgggt gcgatcctgc taatgtcgca tatcggcaac
     5941 tgggagctgg ccgcgcagac gctcaacaaa agaggcctgc cgatcatgct gtatctgggc
     6001 gcgaagcaca aagaacagat tgagcgtatt cagaaagaag ccatggccaa aagcggcata
     6061 aaaatcgtgg caacatccga agacgagaaa tcaccgttcg ccctgatcga cggcatcaat
     6121 tttttgcggg aaggcggcat cgtctccatg gccggcgacc gtttatgggg cgatcagagt
     6181 catatcgcgg tgaattttct cgggcacgag gttcgcctgc cggatacgcc gcatctgttt
     6241 gcattgatga ccggagcgcc gctgatgacg ttttttgttt atcaggaagc cgttggaaag
     6301 tatcatatcc ggatttcgcc tgggcgaaag gttactgccg ccgcacgcgc cgaccggaaa
     6361 aaagccgtgc agaattcggt gcaggcctat gccgatgatc tcgctgaatt tgcccgccag
     6421 catcccttcg agtggcatca tttcgagccg tttttggaaa aatcccactc cgcttcgctt
     6481 cgggaataat ttcatgagcc ttgattttcc gaaacgtagt gcccggaaaa tcttagagca
     6541 aattatcccg ccacccttag ggtggcaggg gcaaacttca caaagctcgt tctaccactg
     6601 cgctacgcgc cggtataatt cgtccagcca gcttcagttt actctgtatc tgaagcttgg
     6661 ggctcattat caacgggtct cattaaaaat ctcttttcgc ttttttaaat ttagtataag
     6721 aaactacata agtaaatgaa aagaaggatt tattcgtgaa agtcaacgaa gtgccgcagg
     6781 ataacacggt tgaatattat gaaggcgtga agcgcgcctg ctatgcggtc gatgaaaaag
     6841 gcaggtatgt cattgtgtcc agtaacggat gggaagcaga ggagcttgtc aatggtctgg
     6901 ctgtaaagga actggctgta aatctggaga aaaccagaaa agccgtactg gccggagaaa
     6961 aaagcccgct cgcctatcac atggaacggc ggcagatgat accggatatc ctgggcaaga
     7021 ccgccggcat tgccgtgttt cgcgtcaagc ggcatctgcg gccggaaatt ttt
//
LOCUS       DAOOLB010000093         7067 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_12607_length_7067_cov_32.167142, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000093 DAOOLB010000000
VERSION     DAOOLB010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 7067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7067
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12607_length_7067_cov_32.167142"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(326..841)
                     /locus_tag="PLF77_09465"
     CDS             complement(326..841)
                     /locus_tag="PLF77_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPC09122.1"
                     /translation="MSELFEKVKLGISKSVAKASVKSKEFLDVTKLKNQISSLQNKKQ
                     DALEELGNIAYAMFLKNNIDTDIIKNKGNEISKIDEQIKNLQNEIVEVQKRAQDSLRE
                     DVPSANTCACGAVIPEGAKFCGVCGSKVETASMSGEHPKDPILESRCKQCQTELEPGA
                     KFCAMCGAKVS"
     gene            1094..1822
                     /locus_tag="PLF77_09470"
     CDS             1094..1822
                     /locus_tag="PLF77_09470"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HPC09123.1"
                     /translation="MPNIQFFALAESVCKKLNLPPIPFPVRKGRDEIIFNPQGVALDL
                     LLDELEQYLIEKPEEKIFYKDAFSRLAAIEGIRLGEEGFHDHAAHYFELGLALDPANL
                     ALRSNYALSLQSAGRKEEAMRQYRFLLQQPVISGQCLVLIPAARLFLDCGDPVTAHQI
                     VKHCASFMPVDNEFWDLFAEARQRCGIKRWMAPRQKQQEIMRQATAPDYKSAPTGKKK
                     NFCPACGNRIKPEARFCGGCGRAL"
     gene            1866..5192
                     /locus_tag="PLF77_09475"
     CDS             1866..5192
                     /locus_tag="PLF77_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09124.1"
                     /translation="MQSVLYAFRVFTPVLILLIILLLFVSHAIADASDYRDKGKENWV
                     LFTFILKDAKTGEPIKGRVYYQSDLAADWFHPDNTKPLLPPGIYRVVGWERHYWQSEQ
                     KLIVNPLKGLQQQIVIDLEPVSSPRDGKSGSPLKVPPILSNLIITGNAAVKMPHMNAS
                     WFGQSSMATFHVYTNAWSEYPGGIGGGSRNSTGHIDYYFFLHTEDAQKHYERLRPGFK
                     VIPNSSQHVSGPTPVSLGDEALQISIDTERPDGKGLLYESRKYLIRDGHFVMNMYINR
                     EIGLDFLTSPEEILKRVRALDVRQVKQMLEPEPYPWPEITPTPGSGKDVDSLGSKNEV
                     ADYASVSPEKALIAVAGVSMVLTLGVLVQLWGMGGLQSPGDIRGALEGLFSSSGGEVT
                     ETESSVPETGAVQEVEVIPKEPEALVPETVIQESRVVETVIPDPVPSGFEYQGKVWYK
                     PPWDKGGPYWMDKSDYDAMRSMMRRGMVWSDRWGWVTPEDGKTMEAQRVAAWDEYKSN
                     TDKDIKVLTDRIAESQEKLAEIREKQAELERIEQLRERLTELERQRELDNSFATQLSD
                     TWDNYVEGVNRDLDALPGELGDMALDAARGIRDTTGALITAAGDTVSDLTNGDNWKAV
                     GKAAVDTAKDLILHPLESSVKVGDVVKDAGSIAIKAADVAGNVAVAIVSDPFAFAESV
                     VGIDNWKKVLDPNVPVGERVSRALYGAVDTALNFASGGTKTAAAGVEAGMDALKTADT
                     VSDAAKAANAADMAADAARITPKLDDPARVQVWEVGRQAGQQKADKLAEAIKSGKPSE
                     IKQALVECQKDKHAIGALNRADDTVKHAYNQQMKQLITDDVDKVVKEKIAQRYGLDPK
                     DVEIVKPTNPTSKPKVGSDLDFTVKVKAKPGEIVPDPNKPGSFIHVKPGDNLTKDVPL
                     KDAQEIYNRELYSKVTGEPIPTDPEKFKAFQAEADKLAHEMDHVAGHRVGAESYGASQ
                     QDLEVILQKSGGRFTDPEQAGMVASYKSTHLYQEAESITKLDPGKAEQLVADGMRQTT
                     KQYSNLIETRVAALQNQGIEVAVDPRLTKAVDIFKQVETKGLPPAKAEALLKSQLNMS
                     KDDVANMLGAQLAKLHRVKA"
     gene            5219..5683
                     /locus_tag="PLF77_09480"
     CDS             5219..5683
                     /locus_tag="PLF77_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09125.1"
                     /translation="MWAVFLKIVSYFFLLLFVLGVVLMLLTWRKEKFVKPKWILFGQI
                     VAFMALVVFTMLSRNPLGFWSWLLIFLAGLCGGYFYGRTVKVKKSARGIMMNYTLPYV
                     ITWGVLLFLTQFLTISTGRIPVIVLGLCVLNTGLNLAMNGQVVWNYNRMKKT"
     gene            complement(5747..6934)
                     /locus_tag="PLF77_09485"
     CDS             complement(5747..6934)
                     /locus_tag="PLF77_09485"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPC09126.1"
                     /translation="MSIAKKIKSSISNSSMIRKMFEEGAVMKRKYGADKVFDFSLGNP
                     NVEPPAVFKRELIRLAAKPLPLKHGYAPNAGFADTRQAIAKQVSKTCDLNLSADHIVM
                     SCGAAGALNVIFKSLLDPNDEVIVPMPYFVEYPFYIDNYGGVTKFVRTRKDFSLDIEA
                     ISKAINKKTKAVLINSPNNPTGKVYSSKNIQALAALLKKKGKTFHKTIYLVSDEPYNE
                     IVYDRVIVPSVLKSYTNSIVACSYSKTLSLPGERIGYIAVNPEIDDCRDLISALILCT
                     RILGFVHAPALMQRIVVKLQNAAVDVNVYQNKRDLLCKGLSKAGYKFAKPQGAFYLFV
                     KSPLPDDVAFTKILLQKNILVVPGSGFGAAGYFRIAYCVDDKTIVNSMRGFTEAIRET
                     SGK"
BASE COUNT         1842 a         1663 c         1754 g         1808 t
ORIGIN      
        1 cgcgtatccg atttaaaacg cgtgccgcct ttgccgatga agtatcctcc gatcgcaatg
       61 cccattaaaa aacatatccc gacaatcagc gccgctatga ttacaattcg attttcattg
      121 tgcgtcattt tacgataccc tccccgatat tgtattattg taaaaaaaat tcacgatgat
      181 ttttaaaata taagtaacga tagcattata cgttacttaa gaaaaagagc gggaaacaat
      241 acttcatagg ttgtaaggtt ttcttatggc gccatttttc atgaacattt tgccttcctt
      301 aacgcctttt aaaaaaggat ttattttatg atacttttgc gccacacatc gcgcagaact
      361 tagcccccgg ttccagttct gtctgacatt gtttacatct gctttctaaa attggatctt
      421 tgggatgttc gccagacatt gatgccgttt ccactttgct gccgcagacg ccacaaaact
      481 ttgcaccctc ggggataacg gctccacatg cgcatgtatt cgcggacggg acatcttccc
      541 ttaaagagtc ctgagccctc ttctgcacct caacaatctc attttgcaga ttctttatct
      601 gctcatcgat cttcgaaatc tcattgccct tatttttaat gatatctgta tcaatgttgt
      661 tcttcaaaaa cattgcataa gcaatattgc ccaattcctc cagcgcatcc tgcttcttat
      721 tttgaagaga agatatttga tttttcagtt ttgttacatc aagaaactct ttggacttga
      781 cgcttgcctt tgcgacactt ttacttatgc ccagcttgac cttttcaaat aactctgaca
      841 tccgaatctc ctttttttac agacattaat catggatgat aaaattataa attgaatgat
      901 ttcacgtgtc aatgagttta aaagtaacaa attacctcaa aaatattcaa atattcatgg
      961 tgtaatacga tcgtcattcc ccgaaaaact caggccgaac cgacattttt tatgtcttga
     1021 atcttcttga cacacaaaaa catttttttc tataattacg cacagaaaaa acattttcta
     1081 aaagaataaa cgtatgccga atatacagtt ttttgcgtta gccgaaagtg tctgcaaaaa
     1141 gctcaatctc ccgccgattc cttttcctgt ccgcaaaggc cgtgatgaaa taatatttaa
     1201 tccacagggt gtcgccctcg atttgcttct ggatgagctg gagcagtatt taatcgaaaa
     1261 gcccgaagaa aagattttct acaaagatgc attctcgcgg cttgctgcca tcgaaggaat
     1321 ccgtctgggc gaggaaggat tccatgatca cgccgcacac tattttgaat tgggactggc
     1381 ccttgatccg gccaatctag cccttcgatc caattacgca ctttcgctcc aaagtgccgg
     1441 ccgcaaggaa gaggccatgc gtcagtaccg ctttcttctg cagcagccgg taatttccgg
     1501 tcaatgtctt gttttgattc cggccgcccg tctctttctg gattgcggtg atccggtcac
     1561 ggcgcatcag attgtcaagc actgcgcgtc tttcatgccg gtggataacg aattctggga
     1621 tctatttgcc gaagcccgac agcgctgcgg aatcaaacgg tggatggcac cccgccaaaa
     1681 gcaacaagaa attatgcggc aagcaaccgc gcctgattat aaaagtgcgc ccacgggcaa
     1741 gaagaaaaat ttctgcccgg cctgcgggaa tcgcatcaag cccgaagcca gattctgtgg
     1801 aggttgcggt cgtgcgctgt aaaaatgatt tttcggttga atgcatgaag gcgaaggcat
     1861 ttgcagtgca gagcgtgcta tacgcatttc gcgttttcac tccggttctc attttgttaa
     1921 tcatcctatt gctttttgtt tcccatgcta tcgcggacgc ctctgattat cgcgataaag
     1981 gaaaggaaaa ctgggtcctg tttacattta tactcaagga cgccaaaacc ggcgaaccca
     2041 taaaaggccg cgtttattat cagtccgacc tggccgcgga ctggtttcat ccggataaca
     2101 ccaaaccact tttgcctccg ggcatctatc gagtagtggg atgggagaga cattactggc
     2161 aatccgagca aaaactgatt gttaatccac ttaagggatt gcagcagcaa attgttattg
     2221 atctggagcc ggtgtcatct ccaagagacg gcaaatcggg ttcgcccttg aaggttccgc
     2281 cgattttgtc gaacctgatc atcaccggaa acgcagcggt aaaaatgccg catatgaatg
     2341 cgtcatggtt cgggcagagc agtatggcca cttttcatgt gtatacgaat gcgtggtcgg
     2401 aatatcccgg cggcatcggt ggaggctcaa gaaacagcac aggccatatt gattactact
     2461 ttttccttca tacggaagac gctcaaaaac attatgaacg acttcgtccg ggtttcaagg
     2521 taattcccaa cagttcgcaa catgtttctg gcccgactcc ggtttcgctc ggtgatgagg
     2581 cacttcagat cagcatcgac acggagcgtc cggatggaaa gggccttttg tatgaatcac
     2641 gaaagtatct catccgcgat ggtcatttcg tgatgaatat gtacatcaac agagaaatcg
     2701 gcttggattt cctgacctcg cctgaggaaa ttctcaaacg tgtccgtgcg ctcgatgtac
     2761 ggcaggtcaa gcaaatgctg gaacccgagc cttatccctg gcctgaaatt accccaacac
     2821 ccggttcagg aaaagatgtt gattctttag gatctaaaaa tgaggttgcc gattatgcct
     2881 ctgtttcccc ggagaaggcg cttatagctg tggccggcgt gtccatggtg ttgaccttgg
     2941 gggtattggt gcagctttgg ggaatgggtg gccttcagag tccgggagat attcgcggag
     3001 cgctggaggg tcttttcagt tcctccgggg gtgaggtgac agagactgaa tcttctgtac
     3061 ctgagaccgg ggctgtgcag gaagtggagg tcatcccgaa agagccggaa gcactcgtac
     3121 ccgagaccgt tattcaggaa tcccgtgttg tggaaacagt tatccccgat cccgttccgt
     3181 cgggcttcga gtatcagggc aaagtctggt ataagccgcc gtgggacaag ggcggtccat
     3241 actggatgga taaatctgat tacgatgcca tgcgctccat gatgcgccgg ggtatggtct
     3301 ggtcggatcg ctggggctgg gtgactccgg aagacggaaa aaccatggaa gcccagcgcg
     3361 tcgcggcctg ggatgaatat aaatcaaaca cggataaaga catcaaagtc cttaccgaca
     3421 ggattgccga atcgcaggaa aagcttgccg agatccggga aaagcaggcc gaacttgaaa
     3481 gaatcgaaca gttaagagag cgcctgactg agctggaaag acagcgggaa ctggacaact
     3541 catttgccac gcagcttagt gacacctggg acaattatgt cgaaggagtg aacagggatt
     3601 tagacgccct tcccggtgag ttgggcgata tggcactaga tgccgcccgc gggataagag
     3661 acacaaccgg cgcattgatt acagccgccg gggatactgt ttctgattta accaatggag
     3721 acaactggaa agccgtgggc aaggcggcgg tggatacggc caaagatctg attctccatc
     3781 cgttggaatc ttctgttaag gttggtgatg tcgtcaaaga cgccgggagc atagccatca
     3841 aggccgcgga cgttgccgga aatgtggcgg ttgccattgt ttccgatccg tttgcctttg
     3901 cggaatcggt cgtgggcatt gacaactgga aaaaagttct tgatcctaat gtgccagtgg
     3961 gagagcgtgt cagccgcgcg ttatacggag cagtagacac tgctttgaac tttgcctcgg
     4021 gtggaaccaa aacggcggct gccggcgttg aagccggtat ggatgcgctc aaaacagccg
     4081 atacggtatc ggatgcagcc aaagccgcaa atgcagcaga catggcggca gacgcggcac
     4141 gcattacccc gaagctcgat gacccggccc gtgtgcaggt ctgggaagtg ggccgccagg
     4201 caggccagca gaaagcggac aaactggccg aagcgatcaa aagcggcaag ccctctgaaa
     4261 tcaagcaagc tcttgtggaa tgccagaaag acaagcacgc catcggagcg ctcaataggg
     4321 cggatgatac cgtaaaacat gcgtataatc agcaaatgaa acagcttatc acggatgatg
     4381 tggataaggt ggtaaaggaa aaaatcgctc agcgctatgg gctggatccc aaagatgtgg
     4441 agattgtcaa gcccacgaat cccacgtcca aaccgaaggt tggcagcgat cttgatttta
     4501 ccgtcaaagt aaaagcgaaa cccggtgaga tcgtgcccga tcctaataag ccgggaagct
     4561 tcattcatgt gaagccgggg gataatctga ccaaggatgt tcccctcaaa gacgcccagg
     4621 aaatttataa tcgggaactc tacagcaagg taacgggaga gccgattccg acggatcctg
     4681 aaaagtttaa agcctttcag gccgaagccg acaaactcgc gcatgagatg gaccatgtag
     4741 cgggacatcg cgtgggagcg gaatcctacg gcgcctcgca gcaggatttg gaagtcattc
     4801 tgcagaaatc cggcggccgg tttacggatc cggaacaggc gggaatggta gcttcctaca
     4861 agtccacgca tctttaccag gaagcggaaa gcatcacaaa actggatccc ggtaaagccg
     4921 aacagcttgt cgccgacggc atgcggcaga ccactaaaca atacagcaat ctgatcgaaa
     4981 cacgggtggc cgcgctgcag aaccagggca ttgaggttgc cgtggatccg cgcctgacga
     5041 aggcggtgga catttttaaa caggtggaaa ccaaaggcct gcctccggca aaagccgaag
     5101 cgttattgaa aagccagttg aatatgtcca aggacgatgt agccaatatg ctcggcgcgc
     5161 agctggctaa actgcacagg gtgaaagctt aatcatgaag atgagggaat tttaaattat
     5221 gtgggctgtt ttcctgaaaa ttgtttcgta ctttttcctg ctcctgtttg tgctgggcgt
     5281 ggtgttgatg cttttgacct ggcgcaagga aaaattcgtc aagccgaagt ggattctttt
     5341 cgggcagatt gtggcgttta tggcacttgt ggtctttaca atgttgtccc ggaatccact
     5401 gggattctgg agctggttgc tcatctttct ggccgggctg tgtggcggat atttttacgg
     5461 ccgaacggtg aaagtgaaaa agtctgcacg cggaatcatg atgaactaca ccctgcccta
     5521 tgtcatcacg tggggcgtgc ttttattcct cacgcagttt ttgacgatca gcacgggtcg
     5581 tattcctgtg attgtgctgg gcctgtgtgt gctcaatact ggattaaatc tggcgatgaa
     5641 tggccaagtc gtctggaatt ataaccggat gaaaaaaacc tgacccctaa ttacgcatca
     5701 aaagaaggct tttgtttgac tttgtcattg atcagaattg catatgctat ttgccggaag
     5761 tttccctgat cgcttctgta aatccccgca tggaattgac aattgttttg tcatcaacgc
     5821 agtaggcgat cctgaaatat ccggcagcgc cgaaaccgct tcccggaaca accaggatat
     5881 ttttctgcaa aagaatttta gtgaatgcaa catcatccgg tagcgggctt ttgacaaaca
     5941 gataaaaggc cccttgcggt ttggcaaatt tataaccggc ttttgacaac cctttgcaca
     6001 gcagatcgcg cttgttctga tacacgttta catccaccgc tgcattttgc agtttcacga
     6061 caattctctg catcaatgcc ggtgcgtgaa caaatcccag aatccgggtg cataaaatca
     6121 gagcactgat taaatcccgg caatcgtcaa tttccggatt gacggcaata tagccgattc
     6181 tttcccccgg cagagaaagc gtcttggaat aggaacaggc aacgatactg ttcgtgtagg
     6241 atttcagcac gctggggact attacccggt cataaacgat ttcattatag ggttcgtcgg
     6301 aaaccaggta aatggtttta tgaaacgttt tgcccttttt cttcaaaagg gcggcaagcg
     6361 cctgaatgtt ttttgaactg taaacctttc ccgtcgggtt attcggtgaa ttgattaaca
     6421 ccgcttttgt ctttttgttg atggcctttg agatcgcctc gatgtccaga gaaaaatctt
     6481 ttctggttct gacgaatttt gttacgcctc cgtagttgtc gatgtaaaat ggatattcga
     6541 cgaaataagg catggggacg atgacttcat cattgggatc cagtaatgat ttgaaaataa
     6601 cattcaacgc accggctgcg ccgcaggaca tgacaatatg gtcggcgctt aaattcagat
     6661 cgcaggtttt actgacctgc ttggcaatag cctgccgtgt atccgcaaaa ccggcgttcg
     6721 gcgcgtatcc gtgcttgagg ggcaatggct ttgcggccag acgaatcaat tccctcttga
     6781 agactgccgg aggctcgacg ttgggattcc ccaggctgaa atcaaagact ttatcggcgc
     6841 cgtattttct tttcatcacg gcgccttctt caaacatctt tctaatcatc gaagaattcg
     6901 aaatcgatga tttaattttt tttgcaattg acaacgaatg tattatcctt tctttcttct
     6961 gaggagcaca aaaatgccca gcaaaatgat aataccgggc aacacatatt tcgaatactg
     7021 ggaaacatcg aatccgtaga actcataaaa gagaaacccc gtcccgg
//
LOCUS       DAOOLB010000094         6978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_12802_length_6978_cov_42.387838, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000094 DAOOLB010000000
VERSION     DAOOLB010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12802_length_6978_cov_42.387838"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2090
                     /locus_tag="PLF77_09490"
     CDS             <1..2090
                     /locus_tag="PLF77_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09127.1"
                     /translation="GGLVGENDGSILNSYATGAVSGDYAVGGLVGDNNDTIMGSVVDD
                     TYEGYTSFAIGKVSGFEDVGGLVGENDGSIWWSKASGAVNGSMTAGGLVGFNYGNILG
                     SLATGAVTANSNVMGAYAFGDTLFTVNGGAVLGGLVGVNEGSIKYSRADGAVSGGSII
                     GGLAGVNDGSIFVAHANGNVTGSSTPSKITVETINDDLGYEPLLTDFTASIDGGNIVG
                     GLVGLNTGMIKKTDANGNVTGGSIVGGLVGLNVDSPCDEERGGKIMMSKATGNVKGGS
                     NAGTIAFDYNGPPPETVGGREGLSFIPVNTNITGSIKGGNIVGGLVGLNAGGKLKGVS
                     AEGNVEGGSIVGGLVGLNAGGQVRGALASGNVTGKNNVGNITLDYTEMPFAPGDVVDL
                     PLTTANVSIKGGNIIGGLVGLNFDSKIEYTLAEGNVTGGNFVGGLVGLNAGALCMGDG
                     AVITDSAAYGNVTGSKSTGAVNVSYNVSDSDEVFEDFNLNLKGGNFVGGLVGLNYDGG
                     IINTHADGNVTGGGDYVGGLVGYNLNTVPEGDHPPTIGALIERSYALGNVTGGNFVGG
                     LVGYNSGVIVDTDAWGNVTGKDAVGGLVGFNDHDSVIVYSYSIGKVSGSTSVGGFVGH
                     NNNGLIENAYWDMTTSGTIVGVGTSNGMPIDVTGLSTPDMKQESNFEGFDFDNVWYID
                     EGVTYPLVGVLAP"
     gene            2209..4011
                     /locus_tag="PLF77_09495"
     CDS             2209..4011
                     /locus_tag="PLF77_09495"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HPC09128.1"
                     /translation="MFPINRKNLFASVIIFFFFIALFALPAAAQERAGEIIQQFEKKE
                     VVPRDLPDMPVIEQKEEKTQEAPAGPKILIKQINVKMHTKAGGEEKALLLLPDAIRTI
                     TSRYENRELSFAEINQIANDITAAYRSRGYLLAYAFIPQQEVKDGLLEIGVIEAQLGK
                     ITVAGNTSYSTDFIRRHLEKIRKDPSIKTDTLQNSLLLLNEYPSLNVIAVMEAGKEFG
                     TTDIVAQVQDSRPVSGSISYDNFGSKYTSENRLNADLNIGNLLGSGDLLTLRGITGLD
                     EIDFDNLSYLRAEYAIPIMYYGTKMGAYYADSRYEVGEEYEPLEIHGKTHLAGLYLTH
                     PLLKTRLHALDLKLGFDYKDIYEYMLDKLASKDKIRVASLGATYNFIDKFQGRNIINL
                     TYHQGIRDLFGGTGKNERESKRQGLWEQLFGSSEINVTRTPTSRLYADGGFSKGTLDL
                     ARIQKLPGYNYMILKGSGQISSDNLFVAEQFFIGGISTVRGFSSASQGGDQGYCVSAE
                     FHLSPVFPEAKVFNQTFGDMFKFVLFADHGGVFKNDVLPGEDEDDYLTSIGAGLRLYM
                     GKHFFARLDYAVPRIDGSFETGDAEIYGQVAFVF"
     gene            4300..4740
                     /locus_tag="PLF77_09500"
     CDS             4300..4740
                     /locus_tag="PLF77_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09129.1"
                     /translation="MKHRIYFKALTFTGCLNLILPLMLLLILSACGMASSSQRVGGPC
                     TYKQYQGDAEIVSVTPVKGKAEEYEIRFSFHPREKIQEEFARVEGKQWLLVREDFSFP
                     KKDFLQQYGIRKGSRFPCVMNVITRGTCTPILFDFPTIRNGRAQ"
     gene            4801..6549
                     /locus_tag="PLF77_09505"
     CDS             4801..6549
                     /locus_tag="PLF77_09505"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPC09130.1"
                     /translation="MKQKFLWPNITVFIILIFFILPVSGQADSTTSGQRERYVLDTNR
                     PVKTFPIMRPDDLTIRKWQSRFERAPRAHIREKYHRRAHHRAKARTDQTGKVSSINLL
                     GLLTYTPSENQGSCGDCWAWAGTRVMGIDLNTQQGSADNLSVQFINSCYGENACCGGW
                     LSDVVDFYNGKLMAISAANTNASFQDSAFRCSYFGAFSRVSCENITTEANYPIQSISE
                     ATVHTTGISQAEAISNIKNVLNQGKAIWFAFFLPDNASWNSFYNFWNNQSENALWDSD
                     NFCGQTWDTYEGGGHAVTLVGYDDSAADTDDHYWIALNSWGTTAGRPNGIFHVKMYIN
                     YNCTLRGLSSYPLGFQTLNIEWGVAQADPVDRTAPVVTAFSVPTVSKSLTVPVNVFTA
                     TDNMEVTGYMITASSAKPSSSAAGWSANAPTRYTFQSGTLGTKTLYAWAKDAAGNVSS
                     YKSASLTLSMPDLIVSAVSNWSTTITASRRSFSITDTNKNQGNAAAPVSVTKYYLSTT
                     PSRTANAILLGGTRSVKAIAVGKTSRGSAKVTVPQGIAKTVYYAIACADDNLVVNESS
                     ESNNCKASSRKITVKY"
BASE COUNT         1797 a         1747 c         1796 g         1638 t
ORIGIN      
        1 tcggcggact ggttggggaa aatgatggat cgattctgaa ttcctatgcg actggagcgg
       61 tcagcggtga ctacgctgtc ggcgggctcg tcggagacaa caacgacacg attatgggtt
      121 cggttgttga cgatacgtat gaaggctata cttccttcgc tatcggcaag gtcagcggct
      181 ttgaagatgt cggcggactc gtcggagaaa acgacggcag tatctggtgg tcgaaagcct
      241 caggcgcggt caacggcagt atgaccgcag gcgggctggt cggatttaat tatggcaata
      301 ttctcggatc actggctacc ggcgccgtca ccgcaaacag caatgtcatg ggtgcttacg
      361 cgttcggcga taccttgttt accgtcaacg gtggagccgt tctcggcgga ctggtcggcg
      421 ttaatgaggg cagtataaaa tattcgagag ctgacggagc cgtcagcggc ggcagtatca
      481 ttggcggtct ggccggagtt aatgatggat cgatatttgt ggctcatgcc aacggcaatg
      541 tcaccggaag cagcaccccc agcaaaatta ctgtcgaaac aatcaacgac gatctgggat
      601 atgaaccgtt actcactgat tttacagcca gcatcgatgg cggcaacatt gtcggcggcc
      661 tggtaggact caacaccggc atgattaaaa aaaccgacgc caacggcaat gtcaccggcg
      721 gcagcatcgt cggcggactc gtcgggctta atgttgattc accttgcgac gaagaacgcg
      781 gcggaaaaat catgatgtcg aaagccaccg gcaacgtcaa gggcggcagc aacgcaggaa
      841 ccattgcgtt tgattataac ggcccgcctc ccgaaaccgt aggtggtcgc gaaggcttgt
      901 cgtttattcc tgttaatacc aacatcaccg gcagcattaa aggcggcaat attgtcggcg
      961 gactcgtcgg actcaatgcc ggcggcaagc tgaagggtgt ctcagccgaa ggcaatgtcg
     1021 agggcggcag catcgtcggc ggactggtcg gactcaatgc cggcggtcag gtcaggggtg
     1081 cgctcgcaag cggcaatgtg accggtaaaa acaacgtcgg gaacatcacc cttgattaca
     1141 ccgaaatgcc ttttgctccc ggtgacgtcg ttgacctgcc cttaacaact gccaatgtca
     1201 gcattaaagg cggcaacatt atcggcgggc tggtcggact caatttcgac agcaaaatcg
     1261 aatacacatt agccgaaggc aatgtcaccg gcggtaactt tgtcggcggg ctggtcggac
     1321 tcaatgcggg tgctttgtgc atgggtgacg gcgcagtcat caccgattca gctgcttacg
     1381 gcaatgtcac cggcagcaag agcaccggcg cggtcaatgt cagctacaat gtttcggaca
     1441 gcgacgaggt ctttgaagat ttcaacctga atctcaaagg cggcaacttt gtcggcggac
     1501 tcgtcggact caattacgac ggcggaatca ttaacacgca cgctgacggt aatgtcaccg
     1561 gcggcggcga ttatgtcggc ggactggtcg gttataatct gaacactgtt ccggaaggtg
     1621 atcatccgcc caccattgga gcgctgatcg aaagatccta tgctctcggc aatgtcaccg
     1681 gtggcaactt cgtcggcggc ctcgtcggct acaacagcgg cgtcatcgtc gacaccgatg
     1741 cctggggtaa cgtgaccggc aaggatgcgg tcggaggact cgttggattc aatgaccacg
     1801 acagtgtgat tgtctattct tattccatcg gcaaggtcag cggcagtaca tccgtcggcg
     1861 ggtttgtcgg tcataataac aacggtctga ttgagaatgc ctactgggat atgacgacat
     1921 ccggcacgat cgtcggcgtc ggcaccagca atggcatgcc aatagatgtg actggtctta
     1981 gcacacccga tatgaagcag gaaagcaact ttgaaggttt tgacttcgat aatgtgtggt
     2041 acattgatga aggggtgacc tatccgttgg ttggtgttct ggcaccataa attaaatttc
     2101 tttaaccttt attccaccat ttcctctcct gcaaatcggg agaggaaatg gtgctttttc
     2161 cgcccggcag atattgtata aatccctatt gagttaacgg agttgaacat gtttcctata
     2221 aacaggaaga atcttttcgc gtccgtcatt atatttttct tttttatagc cctattcgcg
     2281 ctgccggctg ccgctcagga aagggcgggc gagatcatcc agcagttcga gaaaaaggag
     2341 gtcgttcccc gcgatctgcc ggacatgccg gttattgagc aaaaagagga aaagacgcag
     2401 gaggcgccgg ccggcccgaa aattctgatc aaacaaatca acgtcaaaat gcacaccaag
     2461 gccggcgggg aagaaaaagc gcttctgctt ctacccgatg ccattcgtac gattacgtcc
     2521 cggtatgaaa acagggagct gtcttttgcg gaaatcaatc agattgccaa tgacatcacg
     2581 gccgcttacc gcagccgggg ttatcttctg gcgtacgctt ttatcccgca gcaggaagtg
     2641 aaggacgggc tgctggaaat cggagtcatc gaagcgcaac tgggaaaaat caccgtcgcc
     2701 ggcaacacat cctacagcac cgattttatc cggaggcatc tggaaaaaat ccgcaaagat
     2761 ccctccatca aaacagacac cctgcagaac tcattgctgc tgctgaacga atatccctcc
     2821 ctgaacgtca tagccgtcat ggaggccgga aaagaattcg gcacaacgga cattgtcgcg
     2881 caggttcaag acagccggcc cgtgtcggga agtatttcct atgataattt cggctcgaag
     2941 tacaccagtg aaaaccgtct caatgccgat ctgaacatcg gcaacctgct cggcagcggg
     3001 gacctgctga cgctcagagg cataaccgga ctggacgaga ttgattttga caatctgtcc
     3061 tacctccggg cggaatatgc cattccaatc atgtattacg gaacaaagat gggggcttac
     3121 tatgccgaca gccgatacga agtgggcgag gagtacgaac cgctcgaaat tcacgggaaa
     3181 acgcatctgg cgggccttta tctgacacat ccgttgctca agacccgact gcacgctctg
     3241 gatttgaaac tgggttttga ttacaaagac atttacgaat acatgcttga taaactggcc
     3301 agcaaggata agattcgcgt cgccagcctg ggagcaacct acaacttcat cgataaattc
     3361 cagggaagaa atattatcaa cctgacgtac catcagggca tccgggatct gttcggaggc
     3421 accggcaaga acgaacggga atccaagcgg cagggattgt gggaacaact gttcggcagc
     3481 agcgaaatta atgtaacgag aacccccacg agccggcttt acgccgacgg cggtttttcc
     3541 aagggtacgc tggatctcgc cagaatccag aaacttcccg gatataatta catgatcctg
     3601 aaagggtccg gtcagatttc cagcgataat ctgtttgtgg ccgaacaatt tttcatcggc
     3661 ggcatcagca cggtcagagg gttttcatcg gcatcgcagg gcggcgatca gggatactgt
     3721 gtttcagccg aattccattt gtcgcccgtt tttcccgagg ctaaagtttt caatcagacc
     3781 ttcggcgaca tgttcaagtt tgttttattt gccgatcacg gcggtgtgtt taaaaacgac
     3841 gttctgccgg gagaagacga ggacgactat ctgaccagca tcggagcagg gctgagacta
     3901 tacatgggta aacatttttt cgcccgcctg gattacgccg tacccagaat agacggctcc
     3961 tttgaaaccg gcgacgcgga aatctacgga caggtggcat ttgtttttta aaacataatg
     4021 gcattctccg tctggtctga taaaaaacat ccgcattttg aatgtttttg aaatttttat
     4081 aaacgcgttt ttacggcgcc gcaggagagg ggcgaacctt tccatgaaat attcctctcc
     4141 aatccaaccc gaacagattg atgatgcccc gccggaccat caagtttcaa atcaagtcgt
     4201 ttgcaaaaac aacatattga tatataatac ataccggttg cctcttaaag gcaaaaaagc
     4261 aatgaccggt ttgtctgttt tgtgacggag acgtcattca tgaaacatcg aatatatttc
     4321 aaagccctga cgttcacagg atgcctcaat ctgattctgc cgctgatgct cctgttgatt
     4381 ctgtcggcgt gcggcatggc ttcatcctct cagcgcgtcg gcggaccgtg cacttacaaa
     4441 cagtatcaag gtgacgctga aatcgtctcg gtgacacccg taaagggaaa ggcagaggag
     4501 tacgaaatcc ggttttcctt tcatccgcgg gaaaagattc aggaagagtt tgcccgcgtg
     4561 gaagggaaac agtggctgct ggtccgggaa gatttttctt ttccgaaaaa agattttctg
     4621 caacaatacg gtatcaggaa gggaagtcgt ttcccttgcg taatgaacgt aattaccaga
     4681 ggaacttgca ccccgattct gtttgatttt cccacaatac gcaacggcag ggcgcaataa
     4741 gcgtgttaag acttttaatc ccatgcctga ataacacagg caagaccgga ggtcgctgac
     4801 atgaaacaaa aattcctatg gcccaacatt actgttttta tcatattaat atttttcata
     4861 ttaccggtat ccggccaggc ggacagcacc acctcggggc aacgagagcg ttacgttctc
     4921 gataccaacc gtcccgtaaa gacatttccc atcatgcgtc cggacgatct aacgatcaga
     4981 aaatggcaga gcagatttga acgggcgccg cgcgcgcaca tccgtgaaaa atatcaccgc
     5041 cgggcccatc accgggcaaa agcgcgcaca gaccagacag gcaaggtatc ctccatcaat
     5101 cttcttgggc ttttgacgta tacaccttcg gaaaaccagg gatcgtgcgg cgattgctgg
     5161 gcatgggccg gaacccgtgt tatgggcatt gatctgaata cgcaacaagg ctctgccgac
     5221 aatctttcgg ttcaatttat caattcctgt tacggggaaa acgcctgttg cggcggctgg
     5281 ctttccgacg ttgtggattt ctacaacggg aaattgatgg ccatttccgc agccaatacg
     5341 aatgcgtcct ttcaggacag cgccttccgg tgcagttact ttggtgcttt ctccagggta
     5401 tcctgcgaaa atattacaac agaggcaaac taccccatcc aatccatctc tgaagccacg
     5461 gtgcacacga ccggtatttc acaggccgag gccatttcca atattaagaa cgtgctgaat
     5521 caggggaaag ccatctggtt cgcgtttttc cttccagaca atgcctcttg gaatagtttt
     5581 tataatttct ggaacaatca gtcggaaaac gccctctggg attcggacaa tttctgcggt
     5641 caaacctggg acacctatga gggcggcggc catgcggtca ctctggtcgg ctatgatgat
     5701 tccgccgccg atacggatga tcattactgg attgctctca acagttgggg aacaaccgcc
     5761 ggcagaccca acggtatctt ccacgttaaa atgtatatta attacaattg cacgcttcgc
     5821 ggtctttcca gctatccgct tggatttcag acgctgaaca ttgaatgggg tgtcgcacag
     5881 gctgatcccg ttgacagaac agcgcccgtc gtgacagcct tctcggtccc cacggtttca
     5941 aaatcgctta cggtgcctgt caacgtcttt actgcaacgg acaacatgga ggtaaccggt
     6001 tacatgatta cggcttcttc agcgaaaccg tcatcatcgg ctgccggctg gtcggcaaac
     6061 gctccgacac gttatacatt ccagtcgggg acactgggca caaaaacatt atatgcctgg
     6121 gccaaagatg ccgcgggaaa tgtttcatct tacaaaagcg cctcattaac gctgagtatg
     6181 cctgatctga tcgtctcggc cgtgagcaac tggtctacaa ccatcactgc atcccggaga
     6241 agtttttcca ttaccgacac caataaaaat cagggcaatg ctgcagcgcc cgtttcagta
     6301 acaaaatact atctatccac gaccccctcc aggacggcca atgccatact tctcggcgga
     6361 acgcgctccg ttaaggcaat tgcagtaggt aaaacgtcga gaggatcagc aaaggttacc
     6421 gttccccagg ggatagcgaa aacggtctat tatgccattg cctgcgcgga tgataatttg
     6481 gtcgtgaacg aaagcagcga atccaataat tgcaaggcgt catcccggaa aattaccgtt
     6541 aaatactgag gataagacga tattccagca atcgctgatt ttgctgatca aaaccgaaga
     6601 ttattcaaaa agggggttat cctgacaata cgcttctgtt tcacgaaaat gacggtaagg
     6661 aatcggtttt gccggcttat ggcggcatgg tagaaatacc cgtgtttgca gtgatttgcc
     6721 gttcaaagac cctttcaatc cgctgcccag cacacctcaa taagagtaaa ttgcatcttg
     6781 taattaatta caattatggt aatgtgacgc gcgtactttt tcactcaaaa agtgatcgta
     6841 aaaaaactgc aaaatactga aaagtatttt tatctgaaaa gaggacttat gaagaacaca
     6901 tttcaattca ttttgatcat tgtagtgctc gttgcatttt ccttcccgtc gatcagcttt
     6961 tccgcgacgc aggaggtt
//
LOCUS       DAOOLB010000095         6872 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_13104_length_6872_cov_45.917412, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000095 DAOOLB010000000
VERSION     DAOOLB010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13104_length_6872_cov_45.917412"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            291..1373
                     /locus_tag="PLF77_09510"
     CDS             291..1373
                     /locus_tag="PLF77_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HPC09131.1"
                     /translation="MAKIDAFFNLMHDQGASDLHLVAGQQPAIRIRGDIERIKYDVLT
                     NDALKVLLYEITPEHKIKQFEETGDVDFAYEVPNLARYRVNFFRQKNGMAAVFREIPD
                     KIQTCQQLGLPNVIAKLAQLPRGLVLVTGPTGSGKSTTLAAIIDEANRLRRDHIITIE
                     DPIEFVHKSQNCIVNHREVGVHTKSFTAALRGALREDPDIILVGEMRDLETISLAIEA
                     ASTGHLVFSTVHTTSAPKTVDRIIEVFPASEQLQIRSTLADGLRAVISQVLFKRIDVK
                     SRCVALEICIATPAVRNLIRESKTYQIPSMMQTGKKYGMQLLDDAIMDLYNRGWISGD
                     DAYIKANEKARFRPLLRQPPTDFTEA"
     gene            1395..>1753
                     /locus_tag="PLF77_09515"
     CDS             1395..>1753
                     /locus_tag="PLF77_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein"
                     /protein_id="HPC09132.1"
                     /translation="MRKPEIDHILTKMLESYPNISDFIITVGKPFQVEAAGQLVPVPL
                     RPHFEKLTPFQTEIFALNLVNQDHRLMTDLLKEGACDLSYELTGVARFRVNIFSQMGN
                     YSIVLRKLESLVPSCADL"
     assembly_gap    1754..1853
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1854..2336
                     /locus_tag="PLF77_09520"
     CDS             <1854..2336
                     /locus_tag="PLF77_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein"
                     /protein_id="HPC09133.1"
                     /translation="VLSTLHTVDAGQTINRIIGMFPRDEENQIRVRLADAIRWIACQR
                     LLPKIGGGRVAAFETLSTSLRVKDVILHGESEGKTFNDIITQGRPFGMMTFDDCIIDH
                     FSKDLITEETAIAFATNRAVVNRGIDAVKKARGDQAVNIGTLEVDRSYGKAKMDTWKR
                     "
     gene            2374..2886
                     /locus_tag="PLF77_09525"
     CDS             2374..2886
                     /locus_tag="PLF77_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09134.1"
                     /translation="MNTDKLAPESVEEEIDYSGRPFDYRDMDTSSAMICENDPAIRQK
                     LRENLQTLNFNVIEPMTAKEALKYTTFQTFNVIVVNEHYDTDKDGVNQVLKYLETLPM
                     SVRRQVFVVLISSTFATMDYMNTLNRSVNLIIHMEEISEIGLILKKEMEENEYFYHVF
                     KELQRKLGRV"
     gene            complement(2888..3826)
                     /locus_tag="PLF77_09530"
     CDS             complement(2888..3826)
                     /locus_tag="PLF77_09530"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPC09135.1"
                     /translation="MTDEKSTKRDLAEKLLQKGKYDEAIILLEEIHQESPDEDPVLLM
                     LAWAYYDSGKTGQAEKCLMILFDRELERKVFTGFAFDELVRLYKQKKDFPNLVNICER
                     AVAAQPDDVGLLTELGNAYLQAGQADQARLTYEKLIAMEDDNPAFYCLWGEALFAAGL
                     NKESEDAYRKAGQLDPDQLDNYYFRIAVLFQKADNHQEARRLLNKCLNLNPSNPLYYC
                     SLGDSLIGLGKLQDAINAYNQAVEIDQAEADAYYNRLGNSLMKTGNFSEAVAAFQTAI
                     RLEPLPIYYLNLSSAYRKMGLDHEADQTIGKAGKIH"
     gene            4414..5484
                     /locus_tag="PLF77_09535"
     CDS             4414..5484
                     /locus_tag="PLF77_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPC09136.1"
                     /translation="MNKKPGYDFQNIGFIVLLVIITLFFFYIVKPFFFAVFWAALIAG
                     IFMPLHRVLNKKIKNPNLCAGATLVIILLCLIIPIAFLINLLLLEAFDIYKSFDSQGG
                     TWTKTLTATLNTLSQSALFAKLNLDQAFILGKFQDIIKAVSGYVINHISDFTQNTILV
                     IVKVAVMIYTLFYFLRDGEKLLESMIGHIPVNKRYLDTFIHQFLSTAKASLKFTFVIG
                     GIQGFLGGLVFYITGIERALIWGVIMMGLSILPGVGCAFVWVPAGIIMLVLGHTWQGI
                     VILIFGSVIISSVDNLLRPVLLGRDTNMHALLIFLSTLGGLAVLGLSGFVMGPIIAAL
                     FLASWKLFLEFYQEKQEEKIES"
     gene            complement(5488..6084)
                     /locus_tag="PLF77_09540"
     CDS             complement(5488..6084)
                     /locus_tag="PLF77_09540"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPC09137.1"
                     /translation="MKILDKKFYHRDTLRVARELLGKKLVRRVNGLELSGMIAETEAY
                     CGRADSACHAHRGKTQRNAVMFGKPGHAYVYFTYGMHYMLNLVTEEEENPCAVLIRAI
                     LPIDGIAAMEARRKKKGTELTNGPAKLCQALSIDKSLNGWNVTLGSELWVENNKKIPD
                     SKIITTPRIGIDYAKTKDREALWRFILKKDDLEGRPVR"
     gene            6321..>6872
                     /locus_tag="PLF77_09545"
     CDS             6321..>6872
                     /locus_tag="PLF77_09545"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC09138.1"
                     /translation="MDIPRIFNITESAHRIHNPITPEKLAILGSALRLESGGRVLDLG
                     SGSGEMLCAWARDHGIIGTGIDMSRLFTEQAKHRAEELGVADQVTFIHGDAAGFVSDE
                     KVDVAACLGATWIGGGVAGTIELLAQSLKPGGIILIGEPYWRQLPPTEDVAKGCLAQS
                     IADFIMLPELLASFDRLGYDVVEM"
BASE COUNT         1698 a         1623 c         1621 g         1830 t
ORIGIN      
        1 cccctggcct cgacattgcg aacgtcgcgc tctcccagct gagctacatc cccaacctta
       61 agaattcccg ccgggtaatt gcacggaagc atatattatt gatgatcgcg gtgtcaagcc
      121 atagttgcta tgattattga attgacaaaa cagcagggtt tttcttatac tctacgcaca
      181 taaaaaaatg aaatatgttt ttatcgatga atgtgaaatt ttcaaataag atattctttt
      241 cacaaagaaa aaatgataag gaaaatcaaa tcacctcgag gagcaaatag atggccaaaa
      301 ttgacgcttt ctttaattta atgcacgatc agggcgcatc cgaccttcat cttgtcgccg
      361 gacagcagcc ggcaatcaga attcgcggcg atatcgagcg gattaagtat gatgttctta
      421 ccaatgatgc cttgaaagtg cttctttatg aaattactcc ggaacacaaa atcaaacagt
      481 tcgaagaaac cggcgatgtc gactttgcct atgaagtgcc caatctggcc cgttatcgtg
      541 tcaatttttt caggcagaaa aacggcatgg cagcggtttt tcgagaaatt cccgataaaa
      601 ttcagacctg tcagcaactg ggactgccca acgtaattgc caaactggcc cagcttccgc
      661 ggggccttgt actggtgaca gggccgaccg gcagcggcaa atccacaacg ctggccgcta
      721 ttattgatga agccaaccgc ctgcgccggg accatatcat taccattgaa gacccgatcg
      781 aatttgtcca taagagtcaa aattgcatcg tcaaccaccg cgaagtgggg gtgcatacca
      841 aatcttttac tgcggcatta cggggagcat tgcgagagga tccggacatt attctggtcg
      901 gagaaatgcg cgatctggaa accatctctc tggcgattga agcggcctcc accggccatc
      961 tggttttttc caccgttcat accacgagcg cgcccaaaac cgttgacaga atcatcgagg
     1021 ttttccccgc cagcgaacag ttgcagatcc gctcgacact ggccgatggt ttacgcgccg
     1081 ttatttctca ggttttattc aagagaattg atgtcaaaag ccgctgcgtc gctctggaaa
     1141 tctgtattgc cacgccggcg gtccgcaact taatccggga atccaagacc taccagattc
     1201 cctccatgat gcagaccggt aaaaaatacg ggatgcagct tctggacgat gccattatgg
     1261 atctgtacaa ccgcggttgg ataagcggcg atgatgccta tatcaaagcc aatgaaaaag
     1321 cccggttccg tccgctgctc agacaaccgc cgaccgattt taccgaagcg taatgtatcg
     1381 agaaaggatc gcatatgaga aagccggaaa ttgatcatat cctgaccaaa atgctggagt
     1441 cgtatcccaa catttctgat tttatcataa ccgtgggcaa accttttcag gtggaggctg
     1501 ccggtcagct ggttcccgtt ccgctgaggc cgcattttga gaaactgacg ccgtttcaga
     1561 cggaaatttt tgctttaaac ctggtgaatc aggatcaccg tctgatgacg gacttgctta
     1621 aggaaggcgc atgcgatttg tcgtatgaat tgaccggcgt ggcgcgtttt cgcgtgaata
     1681 ttttttcgca aatgggcaat tattcgattg tcttaagaaa gctggaatcc cttgttcctt
     1741 cctgcgccga tctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngtattga
     1861 gtacactgca caccgtggat gcgggacaga cgatcaaccg tattatcggt atgttcccgc
     1921 gggatgaaga aaatcagata cgtgtgcggc tggccgatgc catccgctgg atagcctgtc
     1981 agcgtttact gccgaaaata ggcggggggc gtgttgccgc ttttgaaaca ctgagcacga
     2041 gcctgcgggt gaaggatgtt attctgcacg gcgaatcgga aggaaaaaca tttaacgata
     2101 tcatcacaca gggaaggcct ttcggcatga tgacttttga cgactgcatt atcgatcatt
     2161 tcagcaagga tttgatcacg gaggaaacgg ccattgcgtt tgcgacgaac agagctgtag
     2221 tcaatcgcgg tatcgatgcc gtcaagaaag cccgcggcga tcaagccgtc aatatcggaa
     2281 cgctggaagt cgaccggtct tacggaaaag caaaaatgga cacatggaaa agataatgtc
     2341 cggacttaat aaaataacta tcgggaatgg attatgaaca cggataaatt ggcaccagaa
     2401 tcagtcgaag aggagatcga ttactcgggc cgtccttttg actaccggga tatggatacc
     2461 agctcggcca tgatctgcga gaacgatccg gcgatcaggc aaaagctgag agaaaatctg
     2521 caaacgctta actttaacgt gattgaaccg atgacggcga aagaagctct gaagtacacg
     2581 acgtttcaga cgttcaatgt gattgttgtc aatgaacatt atgacacgga caaggacggc
     2641 gtaaaccagg tccttaaata tctggagact cttcccatgt ccgtccgccg gcaggttttc
     2701 gttgtcctga tcagctcgac gtttgccacg atggattaca tgaacacgtt gaacaggagc
     2761 gtgaatttaa tcattcatat ggaagaaatt tcggaaatag gcctgattct gaaaaaagaa
     2821 atggaagaaa acgaatattt ctatcacgtt ttcaaggagt tgcagcgcaa actgggcaga
     2881 gtttaattca gtggatttta ccggctttgc cgattgtctg atcggcctcg tgatcaagcc
     2941 ccatttttct gtaggcggaa gacaggttca ggtaatagat gggcagcggc tcgagccgga
     3001 tggctgtttg aaacgcggct accgcctccg aaaaatttcc ggttttcatc agagagttac
     3061 ctaatcgatt gtagtaggca tccgcctctg cctgatcaat ttcgacggcc tgattataag
     3121 cgttgatcgc atcctgtaat tttcccagac cgataagact gtccccgagc gagcagtaat
     3181 acaggggatt ggaaggattg aggttcaggc atttattcaa cagtcgccgg gcttcctgat
     3241 gattatcggc tttttggaat aaaacggcaa tcctgaaata gtagttgtcg agctgatcgg
     3301 gatccagttg gcctgccttc cggtaagcgt cttcgctttc tttgtttaat cccgccgcaa
     3361 acaacgcttc gccccacaaa caataaaaag cgggattgtc gtcttccatg gcaatcagtt
     3421 tttcatacgt taaacgtgcc tgatcggcct gtcccgcctg aagataggca ttgcctaact
     3481 ccgtcaggag cccgacgtcg tccggctgcg cggcaactgc cctctcgcaa atatttacca
     3541 gattcgggaa gtccttcttc tgcttgtaaa gacgcaccag ttcatcgaac gcaaagccgg
     3601 taaacacctt cctctcgagc tcgcggtcga acaatatcat taaacatttt tcggcctgac
     3661 cggttttgcc gctgtcgtaa tacgcccagg ccagcattaa aaggacaggg tcttcgtccg
     3721 gtgattcctg atgaatttcc tcgagcagga taatggcttc atcatatttg cctttttgaa
     3781 ggagcttttc cgccagatct ctttttgtgc ttttttcgtc ggtcatcgtt aaaaaaaggg
     3841 gaggatgtct cctcccctta tcaaagacta gcagccgctg caggaagaac cgcaaccgcc
     3901 tccggcggca gacaggctgg atgttacctt aaatccagag ccctgcggtg tttctataaa
     3961 atcaacatta atcggtttcg cttgattaaa aatgtccttg tcaattacaa attttgttcc
     4021 gttttcatca aatacttcat catcttttgc tggctcatcc agagccatcc ccagtgcggg
     4081 gccggatcag ccaatagaca ttgttatccg cacagcggaa tcaggccttt ttgctttgat
     4141 aaaatctttg attacctcca atgccttttc tgtcactgta aacatcaaat atcctccttt
     4201 ttgatgaata ttcattattc atctcgaatg taaaatagtc atacctgaca atatgtcaat
     4261 caaaatcttt ttcttgccca ggggtattta tgaattgaca ttataagttg ctgtagttta
     4321 ttattaatta cgtttttata ccttccgcag caaaaacagt ctgatggcaa tatagacgca
     4381 tcaggattag cgatgatagg tcgatggatg aatatgaaca aaaaacccgg ttatgacttt
     4441 caaaacatcg gttttatcgt tttactcgtc attatcaccc ttttcttctt ttacattgtt
     4501 aaaccttttt tctttgcggt tttctgggcg gcgctgattg ccggtatctt tatgcctttg
     4561 catagggtgt tgaataaaaa aatcaaaaat cccaacttat gcgctggcgc tacccttgtc
     4621 atcattctgc tgtgtttgat tattcctatc gcatttctga ttaaccttct tttgttggaa
     4681 gcttttgata tctataagtc cttcgattcc cagggcggca cctggacgaa aacacttacg
     4741 gcaacgctca acacattgag ccagagtgct ttgtttgcca aattgaacct ggatcaggca
     4801 tttatcctgg gaaaatttca ggacatcatc aaggcggtca gcggatatgt catcaatcat
     4861 atttcggatt ttacgcaaaa caccattctt gtcatcgtca aagttgccgt gatgatctac
     4921 acccttttct atttcctgcg cgacggggaa aagcttctgg aatctatgat cggtcatatc
     4981 cccgtgaata agcgctacct cgatacgttc attcaccagt tcctgtccac ggccaaagcg
     5041 tccctgaaat ttacgtttgt catcggcggc atccagggat tcctgggcgg ccttgttttt
     5101 tacattacgg gcattgaaag ggctctcatc tggggcgtga tcatgatggg gctatccata
     5161 ttgccgggag tgggatgtgc ctttgtctgg gtgcccgccg gaatcatcat gctggtgttg
     5221 ggtcatacct ggcagggcat tgtcatcctt attttcggat cggtgattat cagcagcgtg
     5281 gataatttat tgcggccggt tttgctgggc cgcgacacga acatgcacgc gcttttgata
     5341 ttcctttcca cgctgggcgg gttggccgtt ttaggactgt cgggctttgt catgggaccg
     5401 atcatcgccg ctcttttcct ggccagctgg aaattattcc ttgagtttta tcaggaaaaa
     5461 caagaggaaa aaattgaaag ctgattttta tctgacgggt cttccttcaa ggtcgtcttt
     5521 cttcaaaatg aatcgccaaa gcgcctccct gtcttttgtc tttgcgtaat cgatgccgat
     5581 gcggggcgtc gtgatgattt tagaatccgg gattttttta ttattttcca cccacaattc
     5641 actgcccagc gtgacattcc agccgtttaa agatttgtcg atggacagcg cctggcaaag
     5701 cttagccggt ccgttggtta gttcggtgcc tttctttttg cggcgggctt ccattgccgc
     5761 gatgccgtca atcggtagaa ttgcgcggat caaaacggcg cagggatttt cttcttcttc
     5821 agtgaccagg ttgagcatgt agtgcatacc ataggtaaaa taaacatagg catggccggg
     5881 tttgccgaac atgaccgcat tgcgctgtgt tttgccgcgg tgggcatggc aggcgctgtc
     5941 tgctcggccg cagtaagcct ccgtctccgc aatcatgccg gaaagttcaa gaccgttgac
     6001 ccgccgcacc agcttcttcc ccaaaagctc ccgcgccacc cgcagcgtat cccgatgata
     6061 aaatttttta tccaagattt tcataatgag ttgttctttg gacatattta ccaataaata
     6121 accaacgtga cgtaaggatg ttactatcac aaaaggccaa gtagaaaaag tttatttgaa
     6181 attgattttc aaggatattt taattttctt ttatcaatag atggtgtagg ttaatattaa
     6241 agaatcggaa aaacaaaatt gatatattaa tggttcggcc cttaccgaat ctattcacac
     6301 ccgaaaggag attttctgca ttggatattc cccggatctt caacatcact gaaagtgctc
     6361 accgcatcca caatccgatc acacccgaaa agctcgccat tctcggctcg gcgctgcgtc
     6421 tggaatcggg gggccgggta cttgacctcg gcagcggttc gggggagatg ctgtgcgcct
     6481 gggcacgcga tcacggcatc atcggcaccg gcatcgacat gagccggttg ttcaccgagc
     6541 aagcaaaaca tcgcgctgaa gaactcggcg tcgccgatca ggtcacgttc attcacggcg
     6601 atgctgcggg ctttgtttct gacgagaagg tcgatgtggc agcctgtcta ggggccacgt
     6661 ggatcggcgg gggagtcgcc ggcacgatcg agcttctggc gcaaagcctg aagcccggag
     6721 ggatcatcct tatcggcgag ccttactggc ggcagttacc gccgacggaa gatgttgcca
     6781 aagggtgtct tgcccaatcg attgccgact ttatcatgct tccggaactt ctcgcgtctt
     6841 tcgaccgtct cggctacgac gtcgttgaaa tg
//
LOCUS       DAOOLB010000096         6838 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_13178_length_6838_cov_43.419431, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000096 DAOOLB010000000
VERSION     DAOOLB010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13178_length_6838_cov_43.419431"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="PLF77_09550"
     CDS             complement(<1..551)
                     /locus_tag="PLF77_09550"
                     /inference="COORDINATES: protein motif:HMM:NF038196.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HPC09139.1"
                     /translation="METKLFFYTGTGNSFWTAKKLSLLLHDAETVSMALNLSGRIIAD
                     AEKIGFVFPVHIWGIPAPVIDFLNRLDMDPEKYYFAVAVNAGQVAATLIQLEKLLQQK
                     HVKLSSGFSVCLPSNYIPWGGAEAPAKQKKKFSATLDKINCIASSVRAKKEKPPERGP
                     FWQNILFSAIYRMTFERVPRMDKP"
     gene            complement(561..1025)
                     /locus_tag="PLF77_09555"
     CDS             complement(561..1025)
                     /locus_tag="PLF77_09555"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HPC09140.1"
                     /translation="MKKYVFLSVLFSVFLCQTATADFYKWVDEKGEIQITDYPPPENK
                     AVKNIEIHEVGSEDPLNAEGEDDASKAKNQKKPEIVLYTKNNCPDCEKAKEFLNSKNV
                     PFIEYNMDTDEKAVAKRKEVDQGDEVPFAIINRNQVYGFSESVYNKVLQMQP"
     gene            1278..2420
                     /locus_tag="PLF77_09560"
     CDS             1278..2420
                     /locus_tag="PLF77_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HPC09141.1"
                     /translation="MFGKPVSLFSVFGFEIKVDMSWLILAALITWSLASGLFPEYYKD
                     LPKSAYWWMGIAGAIGLFLSIIVHELTHSIVARHYGVSMKEITLFIFGGVAHMEEDPS
                     SPKAEFMIAVVGPVSSFLLAASAFLLYKVGVYSGWPVTLTGVLNYLSWLNMILAVFNL
                     IPAFPLDGGRILRSALWKWKKDIRWSTNIAAQIGSGFGLMLIIMGIFYIVKGNFVGGL
                     WWFLIGLFVRAAAQSSYRQILARNLFHAKKVRDLMVTNPVTVPRAISLEDFIRDYVYK
                     YHFQAYPVVSFGRLTGCIFLKQVASIPREQWAQHTVGSVAVPCSKEITIGPDEDANKA
                     LDLMNRTGNSRLLVVQGDELEGILSLKDMLALLSLKMELNDMEKKT"
     gene            2745..3383
                     /locus_tag="PLF77_09565"
     CDS             2745..3383
                     /locus_tag="PLF77_09565"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC09142.1"
                     /translation="MANDTLLEPRKIPSQERSRKTVAAIYEAATDIFVNTGYAEATTD
                     QIAEKAGVSIGTLYNYFPGKEAILYGLWEKYEKEIKVITDQADQDIRKKGSFDRSIVP
                     VLLNLALELVSYERVQNRLFISQIGLPETIIQKRRELGLYMEATMEAVFRDFSNVRVR
                     NPKIGVHIIWATVQAVFHDYIISVSDEIKPEDFIDELGDMMSRYIFADEAQE"
     gene            3646..4059
                     /locus_tag="PLF77_09570"
     CDS             3646..4059
                     /locus_tag="PLF77_09570"
                     /inference="COORDINATES: protein motif:HMM:NF015567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09143.1"
                     /translation="MFGTLITTHGNLGDELIKSAELIKGRLENIMHICIDQTKDVEDL
                     KKEISNAIKKLDKGNGVLILTDLFGGTPSNISLSFMKEGKVEVLTGVNLPMMLKLSEA
                     KGNMTLRDFACLVKSYGEKNIMLASEILSKKANRS"
     gene            4135..4644
                     /locus_tag="PLF77_09575"
     CDS             4135..4644
                     /locus_tag="PLF77_09575"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HPC09144.1"
                     /translation="MAKDLNIALVRVDSRLVHGQILEAWVPFIKAKCIIVVNDKVAAD
                     PFLETVIKMAVPSDIEVIISSLDDFVRNYSFAHGSGKKTIVLFGSIADACHAFEHGFR
                     FDRLNIGNVYNNEYKVCCSSSVFLCDQEIIRLEDLLKETGVFVELQRVPKGRAVNIKE
                     ALKDYWRVK"
     gene            4775..5470
                     /locus_tag="PLF77_09580"
     CDS             4775..5470
                     /locus_tag="PLF77_09580"
                     /inference="COORDINATES: protein motif:HMM:NF015566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HPC09145.1"
                     /translation="MFQEIILTAFCGSLLCLDRVFMQAMISRPVVIAPLMGLLLGNLY
                     AGLIIGAIIELIWIDRLPIGTYIPPNDSLTAVLAVAIALISGSATGGVTPQIIALAVI
                     LAIPFGSLAKQMDVMIIQSNNVLSDQALRDAQENNLRAIERKNYLGLLKVFFFYGVFL
                     LAAQAVVIPLVIWGYPRLNEPLLKALSFTYYFLPLLGIAVALNMLKLRNAVPVICAVF
                     LVVSMLLEFFHVL"
     gene            5460..5948
                     /gene="rimI"
                     /locus_tag="PLF77_09585"
     CDS             5460..5948
                     /gene="rimI"
                     /locus_tag="PLF77_09585"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HPC09146.1"
                     /translation="MSCKEISLPLTIDPMKKSDLPEIMKIERESFLSPWTVGMFKREI
                     SSDHSHCLCARIHSDGKEIVAGYIVFWLIIDEAHLHSLAVKKEFRGKGIAFRLMQSMK
                     TIAGKNDVLFQTLEVRASNVEAIKLYRKCGFVVKGVRPLYYSDTHEDALIMWADINPV
                     SE"
     gene            5962..6786
                     /locus_tag="PLF77_09590"
     CDS             5962..6786
                     /locus_tag="PLF77_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HPC09147.1"
                     /translation="MAGNVYIGEIITNDEIQDGCFFMKVRTSALFENPLPGQFVMVRI
                     AGLNDPFLSRPISVYAFTREANFCTVELLYRVVGKGTQIMAGLIEGSYLEINGPLGNG
                     FGTANLKENIILISGGIGIAPLSMLMENIGQTARQASAVTMMYTGFQNKAAVVGLDKM
                     RGICRDVNVCTDDGTLGSKGFVTQYFQKDLKKWTPEKTSIFACGPREMLKALAMILNK
                     SKFDCQVSLEERMACGTGACMGCAVAIKDQTGAMTYKRVCADGPVFHLSDIIWDEI"
BASE COUNT         1681 a         1512 c         1719 g         1926 t
ORIGIN      
        1 ggtttgtcca ttcggggaac gcgttcaaac gtcatgcggt agatggcgga aaaaagaata
       61 ttctgccaga aggggcctct ctccggcggt ttttcttttt tggcccggac cgatgaggcg
      121 atgcagttga ttttatccag cgtggcgctg aatttcttct tctgctttgc cggagcttca
      181 gcgcctcccc agggaatgta gttgctgggc aggcaaaccg agaatcccga agacagtttc
      241 acatgcttct gttgcagcaa tttttccaac tggatcagcg ttgcggcaac ctggccggcg
      301 ttgaccgcca cggcaaaata atatttttcc ggatccatgt ccagacgatt tagaaaatca
      361 atcaccggtg caggaattcc ccagatatga accggaaaaa cgaaaccgat tttttcagca
      421 tcggcaatta tcctgccact gaggttaagt gccatggaaa cggtttcagc gtcatggagc
      481 agcagagaaa gtttttttgc cgtccagaaa gaatttcccg ttccggtata gaaaaatagt
      541 tttgtttcca ttgagccaca tcaaggctgc atttgcagga ccttgttata aacgctttcc
      601 gagaatccgt atacctgatt gcggttgatg atcgcgaagg gcacttcgtc gccctggtcc
      661 acttcttttc ttttcgcaac ggccttctca tcggtatcca tgttgtattc aatgaaggga
      721 acgtttttgg aattcaagaa ttctttggct ttttcgcagt ccggacaatt gtttttggta
      781 taaagcacaa tttccggttt cttctggttt ttggccttcg atgcatcatc ttcgccttca
      841 gcgttcagtg gatcttcgga gccgacctca tgaatttcaa tgttttttac agccttattt
      901 tccggcggtg gataatcggt aatttgtatt tctccttttt catcaaccca cttataaaaa
      961 tcagccgttg ccgtctgaca cagaaaaacg gagaataaaa ccgataggaa gacatacttt
     1021 ttcataacct gacctcacga taagctgttt taagaaatag tgattctttt ccggccggct
     1081 taatcaggct ttcatcaaac gtcacgatca aaccggttac aatataatta aaagtatctt
     1141 aaatcatccg gcgttgtcaa attctttatc gttgcgtctt gcggggctgg aggagagcat
     1201 tccggatgct tctgttaaaa atattgattt tcatctttct tctatattat atgaaacaca
     1261 ttctataagg agcatttatg tttggaaaac cggtaagcct tttttcagtg tttgggtttg
     1321 aaataaaagt cgatatgagc tggctgattt tggcggcgtt gatcacctgg tcgctggcca
     1381 gcggtctttt cccggagtat tacaaagacc ttcctaaatc cgcctattgg tggatgggca
     1441 ttgccggcgc cataggcctt tttttatcca ttatcgtgca tgaacttacc cactccattg
     1501 ttgcccgtca ttacggtgta tcgatgaagg aaataaccct ttttattttc ggcggtgttg
     1561 cgcacatgga agaagatccg tccagtccca aagctgaatt catgattgcc gtggtcgggc
     1621 ctgtgtccag ctttctgctg gccgcctctg cttttttgct ttacaaggtc ggggtctaca
     1681 gcgggtggcc ggtgactctg acgggggttc tgaattatct gtcctggctg aacatgattc
     1741 tggcagtatt caatctgatt cctgcttttc ctttggacgg cggacgaatc ctgcgttccg
     1801 cgctatggaa atggaagaaa gacatccgct ggtcgacgaa tattgccgct cagatcggtt
     1861 caggcttcgg cttgatgctg atcatcatgg gcatttttta tatcgttaag ggaaatttcg
     1921 tcggggggtt gtggtggttt ctgatcggtt tgtttgtccg cgcggcggcg caaagttcct
     1981 accggcagat tctcgcgcgg aatcttttcc atgcgaagaa agtcagggat ttgatggtca
     2041 ccaatccggt caccgtaccg cgggccatat ccctggagga ttttatccgc gattatgttt
     2101 acaaatatca tttccaggcg tatcccgtcg tttcgttcgg gagattgacc ggctgtatct
     2161 ttttaaaaca ggtggcctct atcccgcgtg aacaatgggc tcagcatacc gtggggtctg
     2221 tggctgttcc ttgcagcaag gagatcacaa tcggtcccga tgaggatgcg aataaagccc
     2281 ttgacctgat gaatcgtacc ggcaacagcc gtttgctggt tgttcaggga gatgaacttg
     2341 aggggatcct ttcgctgaag gatatgcttg cgctgctgtc tttgaaaatg gaattgaatg
     2401 acatggaaaa aaagacatga gccctttttt gtccgaatgc agcaaccaca gatcagacga
     2461 tcggcgatca ttttatcaca taatattttt tttcgatgaa gattcattac gatcgtgacc
     2521 gtgcgcgctc cctcttcaac atggatctgt ggtcgacgaa gatttcttgc acagaatggt
     2581 ttcacggtgc atgacccgtc agtgggaatt gagcggcgct tttggcaggt cctgaaggat
     2641 tctttgaaaa tggcgatgat cttttcagct tgactgttca agtaagtttt tttataagtt
     2701 cttacagctt catcgtctta caaagcttcc cggaaggact gtttatggcc aacgatacat
     2761 tgcttgaacc gcgcaaaatt ccatctcagg aacgctcgcg taaaacggta gcggccatct
     2821 atgaagctgc gacggatatt tttgtcaata cggggtacgc ggaagcgacc acggatcaaa
     2881 tagctgaaaa agcgggcgtt tcgataggta ctttgtataa ctatttcccc ggcaaggagg
     2941 ctatattata cggtctttgg gaaaagtacg agaaggagat caaagtcata acagaccagg
     3001 ccgatcagga tatccgaaaa aaaggatctt ttgatcgcag cattgttccg gttctgctta
     3061 atcttgctct ggaactggtc agttatgaac gcgtacagaa tcgactgttc atcagccaga
     3121 tcggtttgcc cgaaaccatt attcaaaaac gccgggagct cggtctttac atggaagcga
     3181 ccatggaagc ggtgttccgt gatttttcca atgtccgtgt ccgaaatccg aaaatcgggg
     3241 tacatattat ctgggcgacg gtgcaggctg tctttcacga ttatatcatc tccgtttccg
     3301 atgaaatcaa acctgaagac tttattgatg aactgggcga catgatgagc cgctatattt
     3361 ttgccgacga agcacaggaa taattgcggc ggatttcggg attcgatcgg ctggatctta
     3421 acggtattat ttcacaacct cctgaagatc gagcagttcc ccaggccctc ttgaatgata
     3481 aattctttct ttccggtgcg gtgaatttcc gaccgataca gcactaaaaa attatttcca
     3541 taaaccttga caattatggt gcaatccatt agcgtaacgc tttaaattcg aaggcaaaga
     3601 cgcggtcatg atcattgcgt aaacgggttg aaaagggggt taataatgtt tggcacactt
     3661 attaccacgc atggcaattt gggagatgaa ttgatcaaat cggctgaact gatcaaaggt
     3721 cggcttgaaa atataatgca tatctgcatt gaccagacga aagacgtcga agatttgaaa
     3781 aaagaaataa gcaatgccat caaaaagctc gataaaggca atggcgtatt gattctgacc
     3841 gatcttttcg gggggacccc gtccaatatt tcgctttcgt tcatgaaaga aggtaaagta
     3901 gaggttctca ccggcgtcaa cctgcccatg atgcttaagt tgtctgaagc gaaggggaat
     3961 atgacgctgc gcgattttgc ctgcctggtg aagagttacg gcgaaaaaaa cattatgctg
     4021 gccagtgaaa ttttaagcaa aaaagccaac aggtcatgaa atccacgccc ttttacccgc
     4081 agatggccgc gctattgaaa agggagtctt aggattccgc gacaggggga aaacttggca
     4141 aaagatctga atatagcttt ggttcgtgtt gatagccggt tggttcacgg tcagatttta
     4201 gaagcctggg tgcccttcat taaggcaaaa tgcatcatcg tcgttaacga taaagtggcc
     4261 gcggatcctt ttcttgaaac agtcatcaaa atggcggtgc ccagcgatat cgaagtcatc
     4321 attagttccc tcgatgattt tgtccggaat tattcttttg ctcacggcag cggaaagaaa
     4381 acgatcgttt tgttcggcag catagccgat gcgtgtcatg cttttgaaca tggtttccgt
     4441 ttcgaccgat tgaatatcgg caacgtttat aataacgaat acaaggtatg ctgctcctct
     4501 tcggttttcc tgtgcgatca ggaaattatt cgtctggagg atttgctgaa agaaacagga
     4561 gtttttgtcg aattgcagag agtgccgaaa ggaagagcgg tcaatattaa agaagcgctg
     4621 aaggattatt ggcgtgtaaa gtaagcagcg catttgctca tatgatatgc tgctttaaat
     4681 tctttgtgtc ttttttcgcg tagagggttt ttgtgttggt tgcggctgta ccggtccgca
     4741 atgcgtttct gttcgaaaat ctggggcgag tgacttgttt caagagatta ttttaacggc
     4801 attttgcggc agcctgctgt gtctggatcg cgtttttatg caggccatga tttcccgccc
     4861 ggtggtgatt gccccgctca tggggctatt gctgggaaat ctgtacgcgg gactgatcat
     4921 cggagcaatc attgaactga tctggatcga tcgtctgccg atcggaacgt atattccccc
     4981 caatgattcc ctcacggcgg tgctggccgt ggctatcgct ttaatctccg gttcggcaac
     5041 agggggtgtt accccgcaaa tcatcgcgct tgccgtcatt ctggccatcc cctttggcag
     5101 tttggccaaa caaatggacg tcatgattat ccagtccaac aacgtccttt ctgatcaggc
     5161 cctccgggac gcgcaggaaa acaaccttcg cgccattgaa cgaaaaaatt atctggggct
     5221 tttgaaggta tttttctttt acggtgtttt tctgcttgcc gctcaagcgg ttgtgatccc
     5281 gctcgtcatc tggggatatc ctcgcttgaa tgaaccgctc ctgaaggccc tttccttcac
     5341 gtattatttt ctgccgcttt tgggaattgc cgtggccctg aatatgctta aattgcgcaa
     5401 tgccgttccc gtcatctgcg ccgtattttt agtcgtgtcc atgcttctgg aatttttcca
     5461 tgtcctgtaa ggaaatttct ttgccgttga ccattgatcc catgaagaaa tccgacctgc
     5521 cggaaatcat gaagatcgag cgggaatcgt ttttgtctcc ctggacggtg ggcatgttca
     5581 agagagaaat ctccagcgat cattcccatt gcctttgcgc gcggatccat tccgacggga
     5641 aagagatcgt tgccgggtac attgttttct ggctgattat cgatgaagcc catctgcaca
     5701 gtctagcggt aaaaaaggaa ttccggggga agggaatcgc ttttcgtctg atgcagtcga
     5761 tgaaaaccat tgccggcaaa aacgacgttt tattccagac gctggaagtc agagcctcga
     5821 atgtcgaagc aataaagctt taccggaagt gcggttttgt agtaaaagga gtccgaccgc
     5881 tttattactc ggacacccat gaagacgcgc tgatcatgtg ggcagatatt aatcctgtca
     5941 gtgaataaaa ggattgtgaa gatggccgga aatgtttaca tcggagaaat cattacaaac
     6001 gacgagattc aggacggctg ctttttcatg aaagtgcgga cgtcagcgtt gtttgaaaat
     6061 ccgctgccgg gtcagtttgt gatggtgcgc atcgccggat tgaatgatcc ttttctgtcc
     6121 cgtcccatca gtgtttatgc atttacgcgc gaggccaatt tttgtaccgt tgaactgctc
     6181 tatcgagtgg tgggcaaggg aacgcaaatc atggcaggtt tgattgaagg ttcttacctg
     6241 gaaatcaacg ggccgctggg caacgggttc gggacggcaa acctcaagga aaatattatt
     6301 ttgatttccg gcggtatcgg tattgcaccg ctgtcgatgc tgatggaaaa tatagggcag
     6361 acggcccgtc aggcttcagc ggtcacgatg atgtatacgg gctttcagaa caaagccgcc
     6421 gttgtcggcc tggataaaat gcgcggaata tgccgcgatg tcaacgtctg taccgatgat
     6481 ggaacgctgg gtagtaaagg ttttgtcacg cagtattttc aaaaagactt gaaaaaatgg
     6541 acaccggaaa agacgtcaat ttttgcctgc ggcccccggg aaatgctcaa agcgctggca
     6601 atgatattga acaagagcaa attcgactgt caggtgtcac tggaagagcg catggcctgc
     6661 gggaccggag cctgcatggg ctgcgcggtg gcgataaagg atcagacggg cgcaatgacc
     6721 tacaaacggg tatgcgccga cgggccggtt tttcatctat ccgatatcat atgggatgaa
     6781 atataatgac gaacaaaaac acaaaaccat ccccgaaaat agccgtcagc ctcggacg
//
LOCUS       DAOOLB010000097         6785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_13339_length_6785_cov_62.278306, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000097 DAOOLB010000000
VERSION     DAOOLB010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13339_length_6785_cov_62.278306"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="PLF77_09595"
     CDS             <1..437
                     /locus_tag="PLF77_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPC09148.1"
                     /translation="KFTMKDGAPFKKWVAEIDDFVAKKKTDAFAAEFAMMAEAMTAFK
                     EIVHMNDAWTKGDRQMKQLFATRTLHAGARVYCGMLLLDQALLASARMKEKGEIDADF
                     YKGKIATARFYIMNHVPELFGYLKSMKFGDRSAIDIAEESFM"
     gene            1214..2338
                     /locus_tag="PLF77_09600"
     CDS             1214..2338
                     /locus_tag="PLF77_09600"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HPC09149.1"
                     /translation="MAVVSLEKSNTGRQTEFDLLKVILIIGMVFVHVYEKLSIYDTSV
                     LPGTTAEWVLQVGGGLVGASLFMFCMGIGMAYTPRQTPAAFANRGWHLLFYGYLLNLV
                     REGIPLLIISLSGTGSPEPKAYLSALMNVDILHLAGLSFLLVALFMKFRIAPPVMLLI
                     ALVLQTAAGLWTEATTGTPVMDMLVGLFVYVNSDTSFPLMLWFVYPTFGYTCGNLLKY
                     VADKPKFYRKLLFGIPPVFVCLWVALSLTGLNVPELFVLAGDAQYRQNMMISTLSFLA
                     FFMFSAVVFFAMRSVRNEVLLRFISSTSANLINIYCIQWVIILWLQMLFGVNKTLTAA
                     WIVPAALAILIISIFFAALYKNILARIRHAKENRSKGIKR"
     gene            2724..3800
                     /locus_tag="PLF77_09605"
     CDS             2724..3800
                     /locus_tag="PLF77_09605"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPC09150.1"
                     /translation="MTFKTKLFTFLFAVSIFVFYGNVWSDDSVRYADLGDFRLENGNV
                     IQNCRLGYLTRGALNEEKSNVVLVSTWLAGTSKELADLGFIGPGKVFDSSKHYIIAVD
                     AFANNVSSSPSNSALQKGKAFPRFSLRDLVRAQHLLLTRHLNIRHVRAVSGISMGAMT
                     TFQWMVSYPDFMDRAVPIAGSPWMTSYELLFWSAQMGILEQIGECKGSEEAMKALAPL
                     HVMQVWPPDYRTANTSAAAFPVFLADQQEKLSRYDAYNWSRQVTAIMSHDILKDFGGD
                     RQKAAAAVRAECLVITSAQDQVTSREEAKAFARLIGAQTADLNGVCGHFAFLCDQESL
                     KKRLDAFLAQETGVSPAAAENNKK"
     gene            3939..5366
                     /locus_tag="PLF77_09610"
     CDS             3939..5366
                     /locus_tag="PLF77_09610"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPC09151.1"
                     /translation="MPTSFRIFIISVVSLYFLPSFDGTRLVVSLPVMAEQFSTGTGLV
                     SWILVAYLLAFISSMLILGRFSDIIDIRKIFTLGVFLFTLFSILCGVAQTVLSLIVFR
                     FFQGLGGAMLVVASYSAVARYLSGDLVVRGYAALNIGSAIAMAIGAPVGGLITEYLGW
                     RWNFYINLIMALCFYFIAIRSIPVDEKRTAAKPSIDIASAVSGFLMPFFFMLFISFFK
                     ELGYRSILLWAFLGAGVLSAGLFIIRNNRIANPLLDVNLFKIKGFGLVVILITIPIIL
                     QGGHYFLMPFYFDDIKNMRIVYYGALLMIYSLVFVITDLLSPFIFSRFHFRTIGTAGF
                     ICMMTGSAVMIFGLHTDGLVVPIAYLVMMGMGFGIYNAPSTSIFMNMVSSDRKGLFAG
                     IFQIILRSSLAIGIILFETIYSAGIGNFSETLTRDSAISSDYLNALHFSYKMTFTASF
                     FLIVIALIVLYFPMFKILKLRRKGK"
     gene            5622..6431
                     /locus_tag="PLF77_09615"
     CDS             5622..6431
                     /locus_tag="PLF77_09615"
                     /inference="COORDINATES: protein motif:HMM:NF014635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HPC09152.1"
                     /translation="MQSNIAQALSLRFPPLAINYTWEPPADAAELHPLCSMLLLARAA
                     KGETVAITKGTCGCHGALEGFGLGRSRPEDFPGGRECFLRFLSVGNEGWEQGRAVIQK
                     LTEANAPKILITEFAEGEGFKKTPELVDEWQDEVPDIAPQGSHVVIRPLASLEAGEKP
                     ELVTLLVDPDQLSALVVLANYARPGIDNVRIPFCAGCAGIALYPFDEARQARPRAVIG
                     LTDISARFYLRKLLGKNILSFTAPWSLMKEMDGNVSESFLNRFAWKTMMGR"
     gene            6577..>6785
                     /locus_tag="PLF77_09620"
     CDS             6577..>6785
                     /locus_tag="PLF77_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09153.1"
                     /translation="MEIEFRYDMTGVDWKDVAQTLKSVGMSYHEPDEHRRAFAASRTT
                     VFAYREGRMIGFGRAIADGVYQAAL"
BASE COUNT         1544 a         1663 c         1768 g         1810 t
ORIGIN      
        1 gcaagttcac catgaaggac ggcgcgccgt tcaaaaagtg ggtggcggaa atcgacgact
       61 tcgttgccaa aaagaagacg gacgcattcg cggcggaatt cgcaatgatg gcggaagcca
      121 tgaccgcttt caaagagatc gtccatatga atgacgcctg gacgaagggc gaccgccaga
      181 tgaaacagct ttttgccacc cggaccctgc atgcaggcgc gagagtttac tgcggcatgc
      241 tgcttctgga ccaggcgctt ctggcgtcgg ccaggatgaa agagaaaggc gaaatcgacg
      301 ccgatttcta caagggcaaa atcgccacgg cccggttcta tatcatgaat cacgttcccg
      361 aactcttcgg ttatctcaag tcgatgaagt tcggcgaccg cagcgcgatc gacatcgccg
      421 aagaaagctt catgtagggc gaacgcaccc tgtttacggg tattgggaac ggcctgtgac
      481 ctctcgtgcc cttcagcgta ttaggttatc gtgaccgttc taaggcaatg tagggaaaag
      541 tcgcgactgt tccgagagta tgcagggaac ggtttttaaa ccgttcctcg actgtaagat
      601 ccctcccttg atgggaggga ttccatggag ggtctcttca ccttcaaaga gttgtctgca
      661 cggtttctcc gtaagaggga tttttatccg taggatttct tgtgacatcg ccacggtaag
      721 gtccctccct tgatgggagg ggcaggggga gggtgaactc tttcaccaaa agatgagacg
      781 tccacggaga atctggcgcg ttagatcatc cgggcgtcat tctctcaggt gtgcctgcgg
      841 tatctggaac aggaagggaa tgtggtttat cgcgctgatg atctcgctca tacgccacat
      901 ccacaagccg acgcttgcgc cgaccccgaa tactcatggg atgaatacat tcagtcataa
      961 cgcgttgtga aaaaaatgca tgagggtgat gtttgctcaa atttcggtta aattggccat
     1021 cctggcatcg gaatagatca aaaggcatgc agatttctca ttagaacgaa tattgccaaa
     1081 taccttcagc gcgctcatcc gcgctgtacc ccacatattg tggcaaaaca ttcttgacac
     1141 acaagagacg gcttaatata cttcggctca caaaaaggaa gttcatatca attatcaatt
     1201 gagcgagatg aatatggccg ttgtatcttt ggagaaaagc aacactggaa gacaaacgga
     1261 atttgatctg ctgaaggtga tcctcatcat cggcatggtt ttcgtgcatg tgtacgagaa
     1321 actcagcata tacgatacca gcgtcctgcc gggcacaacc gccgagtggg tcctgcaggt
     1381 aggcggcggt ttggtcggcg cctcgctctt catgttttgc atggggatcg gcatggcgta
     1441 caccccccgg caaacgccgg ccgctttcgc caatcgcggc tggcatttgt tgttttacgg
     1501 ttatctgctc aatctcgtgc gcgagggcat acccctattg atcatttcgc tttcgggaac
     1561 cggatctccg gagccgaagg cgtatctgag cgcgctgatg aatgtggata tcctgcattt
     1621 ggccggcctg tccttccttc tggtcgcact tttcatgaaa ttccggatcg ctccaccggt
     1681 gatgctctta atcgccctcg tcttgcagac ggcggccggc ctgtggacgg aagccacaac
     1741 aggaacccct gtcatggaca tgctggtcgg gctttttgtt tacgtgaatt ccgacaccag
     1801 cttcccgctg atgctctggt tcgtttaccc gacattcgga tatacctgcg ggaatcttct
     1861 gaagtacgtc gcggacaagc cgaaattcta tcgaaaactg ctgtttggca ttccacccgt
     1921 ttttgtgtgc ctgtgggtgg cgctcagcct gaccggcttg aatgttccgg agctgtttgt
     1981 gctggccgga gacgcgcagt accggcaaaa catgatgatc agcacgctgt cttttttagc
     2041 cttctttatg ttttccgctg tcgtattttt tgccatgcgc agcgtccgca acgaagtcct
     2101 gctgcgtttc atctcgagca ccagcgccaa tcttatcaac atttactgta tccaatgggt
     2161 tattatcctg tggctgcaga tgttattcgg cgtgaataag acccttaccg ccgcatggat
     2221 cgttcccgct gcgctcgcga tcctgatcat cagtatattt tttgccgcgc tttacaaaaa
     2281 tatcctggcg cggatccgac acgcaaaaga aaacagatcg aaagggatca aaagatgatt
     2341 cgattgaacc ggccaggccg cagcggaatt aatcactccg aagatttgca tcttcaaccg
     2401 tcaaaaatag gcgggcgctc tacctttgtc ctgacagcaa acgcaacctc aaaccaggtt
     2461 gcagttcttg tttttaaaag tacgaattcc gctgaaaaag acaggcgatt ccgggagaag
     2521 gcggcatcct gctgcctact gaaaaagctg accgtggtgt aaagagcatt cctgccgggc
     2581 atgaagtgcg ccaccgacta cgggttcctt atttgcccgt gccattgccg tttaagcttg
     2641 tttgtttagg cttttttgtt ccggattgac tgttcacatc gttttgctta tgatggctca
     2701 accttttttt caggagtcgt cccatgacat ttaagacaaa attattcaca tttttgttcg
     2761 ccgtcagtat ctttgttttt tatggaaacg tttggtcgga cgattcggtc cggtatgcag
     2821 acctgggcga tttccgactg gaaaacggga acgtaattca gaactgtcgt ctgggctacc
     2881 tcacccgcgg agccttaaat gaagaaaaat ccaatgtcgt gctggtttcc acctggcttg
     2941 ccggcacatc aaaggaactg gcagatctcg gtttcatcgg tcccggcaag gtgttcgaca
     3001 gttcgaagca ctatatcatt gcggtggatg ccttcgccaa caacgtgtcg tcctcgccct
     3061 ccaacagcgc gcttcagaaa ggaaaagcgt ttccgcggtt cagcctccgc gatctggttc
     3121 gcgcccagca tcttctcctg acccggcatt tgaatatccg ccatgtccgc gccgtgtcgg
     3181 gcatctccat gggagccatg acaacgttcc agtggatggt ttcctatccg gattttatgg
     3241 accgggccgt tcccatcgcc ggcagtccct ggatgacatc ttacgaactg ctgttctggt
     3301 cggctcagat ggggattctt gagcagatcg gggaatgcaa gggaagtgaa gaggccatga
     3361 aagccctggc gcctttgcat gtcatgcagg tctggccgcc cgactaccgt acagccaaca
     3421 cgagcgccgc cgctttccct gtctttctag ccgatcagca ggaaaagctt tcccgctatg
     3481 acgcatacaa ctggagccgt caggtcacgg ccattatgag tcatgatatt ctaaaagatt
     3541 tcggcggcga ccgccaaaaa gctgcggctg ctgtgcgcgc cgaatgcctg gtgataacgt
     3601 ccgcccagga tcaggttaca agcagggagg aagcgaaagc attcgcccgc ctgatcgggg
     3661 cgcaaacagc cgacctaaac ggcgtctgcg ggcattttgc cttcctgtgt gatcaggaaa
     3721 gtttaaagaa gagacttgat gcttttttgg ctcaggaaac gggcgtttct cccgcggccg
     3781 ctgaaaacaa caaaaagtaa aaaagagcat ccattcgtgt cataaatggc gaacgtgcaa
     3841 ttacatgcat agaatcctct cgacgcacag gaaatccgtt gctgaatatc atttgcccca
     3901 tcaaacagga agctcttact cattaaaagg tggagaaatt gccaaccagt ttccgaattt
     3961 tcataatcag tgtcgtttct ttatattttc tgccgtcttt tgacggtaca aggcttgttg
     4021 tttcgcttcc tgtcatggcc gaacagtttt ccacgggaac cggtctcgta tcatggattt
     4081 tagttgccta tttgttggcc ttcatttcat ccatgcttat tcttggcaga ttttcagaca
     4141 ttatcgatat tcgaaaaatt tttacactcg gggttttcct gttcacgcta ttctcgattc
     4201 tttgtggagt tgcgcagact gttcttagcc tgatcgtttt tcgcttcttt cagggtttgg
     4261 ggggggcaat gcttgtcgtt gcgtcgtatt cagcggttgc cagatatctc tccggcgatc
     4321 tggttgtcag gggttatgcg gctttaaata ttggttcagc gattgccatg gctattggcg
     4381 ctcccgtggg agggttaatt acagaatatc tggggtggag atggaatttt tacatcaacc
     4441 tgattatggc gctatgtttt tatttcattg cgatacggag tattcctgta gatgaaaaac
     4501 gtactgctgc aaaaccgagt attgatatcg cctctgccgt ctccggattc ctaatgccat
     4561 ttttctttat gctttttata agtttcttta aggagttagg atacagatca atcctgctat
     4621 gggctttttt gggggcaggg gttttatctg cggggctttt tatcatacgg aataatcgca
     4681 tcgccaatcc cctgctggac gttaacttgt tcaaaatcaa aggcttcggc ctggtggtca
     4741 ttctcattac gatcccgatt attttacagg gagggcatta ttttttaatg ccattttatt
     4801 tcgatgacat taagaatatg cgaatagtct attatggcgc gctactgatg atctattcac
     4861 tggtatttgt cataacggat ttgttaagcc catttatttt ttcaagattt cattttcgta
     4921 cgattggaac ggcaggtttt atttgtatga tgacgggaag cgcggttatg attttcggcc
     4981 tccataccga tggcctggtt gttcctattg cctatcttgt gatgatgggg atgggatttg
     5041 ggatttacaa cgcgccttcc acatcgattt ttatgaatat ggtttcgtcc gatcgcaaag
     5101 gactttttgc gggaattttt caaataatac tccggtcttc tctggcaatc gggatcattc
     5161 tttttgaaac aatttattca gcgggtatag ggaacttcag cgagacattg acaagagatt
     5221 ctgcaatttc gtcagattat ctgaacgcat tgcatttttc atataaaatg acatttacag
     5281 ccagtttttt cttaatcgtc atcgcactga ttgttctata ttttccgatg tttaaaattt
     5341 taaaattaag acgcaaagga aaatagacga tcgaaaagcg ggtgccattt accggactgt
     5401 ggggattcgg gggactcact taattgcatt tttctacgca acccttcatg gtttcccaga
     5461 tctaatcatc aattttactt atcgtgggtg tcctgcaggt cggcactgaa taagaaaatc
     5521 actttatctg cctccaccgt tcaaaaattt cgcctttgtg atatatagag gacaaaaccg
     5581 gcggcaaggg ttgccggcag tttcccggta aggagatgga tatgcaatca aatattgcac
     5641 aggcgctgtc cctgagattt ccccccctgg cgatcaatta tacctgggag cccccggcgg
     5701 acgccgcgga actccaccct ttatgctcca tgctgctttt ggcccgggcc gccaaaggag
     5761 agaccgttgc gattaccaag gggacatgcg ggtgccacgg cgcgctggaa ggattcggcc
     5821 tcggacgctc acgccctgag gatttcccgg gcggccgcga atgtttttta cgcttccttt
     5881 ccgtcggcaa cgagggctgg gagcagggcc gcgccgtgat tcaaaagctg accgaggcga
     5941 acgcgccgaa aattctgatc acggaatttg ccgagggcga gggttttaaa aagacgccgg
     6001 agctggtgga tgaatggcag gacgaggttc ccgacattgc cccgcaggga tctcacgtag
     6061 tcatcaggcc gcttgcttct ctggaagcgg gtgaaaagcc ggagctggtt acgctgctgg
     6121 tcgatccgga tcagctttcc gctctggtcg ttctggccaa ctacgcacgt ccgggtattg
     6181 acaacgtgcg gatccctttt tgcgcgggct gcgcgggcat tgcgctgtac ccgtttgacg
     6241 aagcgcggca ggccagaccc cgcgcggtga tcgggctcac ggatatatcg gcccgtttct
     6301 acctgcgcaa acttctggga aaaaacattc tttcgtttac cgcgccctgg agcctgatga
     6361 aagaaatgga tggaaatgta tcggagagct tcctgaaccg gtttgcatgg aaaaccatga
     6421 tgggccggta agcttccgtt gcgggtccgc aaactgaacc ctctacggcg atcccggcct
     6481 tggcggcgac ccgaccgcta atttcggaaa acagagccca cgctttgcaa taataaagag
     6541 ggtgatggcc catcaggaaa agaaatcgga tttgtgatgg aaattgaatt caggtacgac
     6601 atgaccggcg tggattggaa ggacgtggcg cagacgctga aaagcgtcgg tatgagttat
     6661 catgagcctg acgaacaccg cagggcattt gccgcaagcc gtacgacggt ctttgcttat
     6721 cgggaaggca ggatgatcgg attcggccgg gcgatcgcgg acggagttta ccaggcggcc
     6781 cttta
//
LOCUS       DAOOLB010000098         6687 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_13618_length_6687_cov_43.249095, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000098 DAOOLB010000000
VERSION     DAOOLB010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6687
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13618_length_6687_cov_43.249095"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..941)
                     /gene="recO"
                     /locus_tag="PLF77_09625"
     CDS             complement(165..941)
                     /gene="recO"
                     /locus_tag="PLF77_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPC09154.1"
                     /translation="MTAVREHFKTTGFVLRTLSYSESDLIVSFYSHDFGKLKGIAKGA
                     KRSKKRFANVFEPFSLTDIIFTRKSRDTLAFIESCDIVDHYDAIRANMEKTLIASYFI
                     DLTDHFSPEGKKNEDIFRLLQDFLVLLGREKVSDSIVRFFEIRLLKLAGFEPALDHCV
                     LCRTPVTNGNAYFFNAGRGGITCSACARTQKNEQPISAGTLRTLLLGKDMDIDKIKLV
                     TLTDSSATESRCLLTGFISHVLGREVKSWKVLEQVRRYCT"
     gene            complement(928..2226)
                     /locus_tag="PLF77_09630"
     CDS             complement(928..2226)
                     /locus_tag="PLF77_09630"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPC09155.1"
                     /translation="MKLLLTGARVIDPAQKIDSRLDVFLENGKIARVGANLSKSTKSK
                     DAEKITKIIDLAGMIIVPGLIDMHTHLREPGFEYKETVASGAAAAVAGGFTSIACMPN
                     TNPVNDNRSITEFIKAKAAEANLANVYPIGAISRNSDGDQLTEFWDLKEAGVIALSDD
                     GKPVMNAALMRRAMEYAHSLQLPVINHCEDIHLSANGAMNESYYSTILGLNGIPAIAE
                     EVMVARDILIAEYTGTSVHIAHVSTAGSVRLIRSAKERGVNVTAETAPHFFTLTDETL
                     QGYDTFFKVNPPLRGEKDVKAIKEGLADGTIDVIACDHAPHARTDKEVEFEYAANGIS
                     GLETSLSLSLNLIHSKILSWPELVSRMSTRPARILNLPKGTLKEGADADITVIDPLLP
                     WTVDVQVFRSRGKNSPFHGRQMQGKALMTIVGGEIKYDGR"
     gene            complement(2223..3149)
                     /locus_tag="PLF77_09635"
     CDS             complement(2223..3149)
                     /locus_tag="PLF77_09635"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041585001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HPC09156.1"
                     /translation="MKFERKDILGIKDLSVDEINLILETAGSFLEVSTRKIKKVPTLR
                     GKTVINLFYEASTRTRTSFEIAGKRLSADTINISSSTSSVMKGETLIDTARNLEAMNP
                     DVIVIRHSAAGAPHLLASLVKQSIINAGDGAHEHPSQALLDMMTIKERKGNFPGLKVA
                     IIGDIEHSRVVRSNIYGLSKMGATVVVAGPATMLPRDIEQMGVKVFDKMEDAITDADV
                     VMMLRIQLERQKQNIFPSLREYSQRYCLNRNNIKLAKKDVIVMHPGPINRGVELSPDI
                     ADDPSFSVILDQVNKGVAVRMALLYLLTGGSE"
     gene            complement(3173..3721)
                     /gene="pyrR"
                     /locus_tag="PLF77_09640"
     CDS             complement(3173..3721)
                     /gene="pyrR"
                     /locus_tag="PLF77_09640"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003547.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HPC09157.1"
                     /translation="MSNTKSTKTVMDKEGIERCLTRIAYEILEKNKGMENLVLVGIRT
                     GGVYLAERLQKKMSEIEHGKIPMGILDVTLYRDDISVSNKKPRLGETKINFSLDEKIV
                     ILVDDVLFTGRTIRAAMDALIDFGRPRMIQLAVLIDRGHRELPIRADFVGKNLPSSLW
                     EAVRVDLVEKNGVDEVVIEEGT"
     gene            complement(3978..4598)
                     /gene="lepB"
                     /locus_tag="PLF77_09645"
     CDS             complement(3978..4598)
                     /gene="lepB"
                     /locus_tag="PLF77_09645"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPC09158.1"
                     /translation="MTEENKTKSKVKEYVESIIIAILIALFIRTFIICAYKIPSRSMV
                     PTLLVGDHILVNKFIYGVKIPLLRRTIIPVSEPKRGEVVVFIYPNDRSKDFIKRVIGV
                     AGDKIEIKNKKIFINDEEFKDSYGIFSDSLVLPQSLQPRDNFGPIIVPEKSIFVMGDN
                     RDESLDSRFWGFVNLKDVEGKAFVIYWSWNRDENNLRWNRLGQLLH"
     gene            complement(4614..5066)
                     /locus_tag="PLF77_09650"
     CDS             complement(4614..5066)
                     /locus_tag="PLF77_09650"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HPC09159.1"
                     /translation="MNFVDLPGTVVIFFDVVLYAILTGFQQVGWQIIVLLLICAVVAE
                     SLEFFWTIRETPPQEHRPLKSLKATIIGAVTGAFLLTPFWAAPGAFAGFLLGGLAGIL
                     MMEIIRHCEAKIHHWTLSSVIFNTIGKNAVKGFISLFMIAFSLSNIYS"
     gene            complement(5131..6039)
                     /gene="secF"
                     /locus_tag="PLF77_09655"
     CDS             complement(5131..6039)
                     /gene="secF"
                     /locus_tag="PLF77_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HPC09160.1"
                     /translation="MQLIKPGINLDFVGKIKYAITFSVILIIVSIGSVIYHGGLNPGI
                     DFAGGSVIQVKFSKNISADQIRGALKSTRLQNSTIQQFGTNEFLIRTSESFSDQTVLA
                     ANVEQPLAAAFSKDFEIRRVEVVGSKVGADFKRKAVIAVVLSWIAMLIYVGFRFEFRY
                     GVGGIVALVHDVIITIGAVSIMNMEFTISILAALVFLIGFSINDTIVTLDRIRENVKL
                     NPKRQLRETINLSINQTLSRTVLTSLTVFLTVLALYLFGGAVIRDFAFALLVGVVIGT
                     YSSIYIACTIVLMFENLQISKMKRTP"
     gene            complement(6068..>6687)
                     /gene="secD"
                     /locus_tag="PLF77_09660"
     CDS             complement(6068..>6687)
                     /gene="secD"
                     /locus_tag="PLF77_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HPC09161.1"
                     /translation="GNFTMEEARDLAIVLRAGALPAPVNILEERTVGPSLGSDSIRQG
                     VMATMIGSLLVILFLIIYYRLSGAIADVALITNIFLVLAILAAFRATLTLPGIAGLLL
                     TVGVAVDANILIFERIREELRTGKTIRIALDTGYNRAFITIIDTHITGIVAAAFLIMF
                     GTGPIKGFAVTTIIGLLASLFTAVFVTRVIFDYITTNFNIKKLSI"
BASE COUNT         1618 a         1645 c         1717 g         1707 t
ORIGIN      
        1 ccggcggcta aatcggtcag agacatttcg gatgaacttg cccgcaggct caatccttcc
       61 acgccgacat aaacggcatc ggctccgtag agcagggctg tgcgcaattt ttccagattc
      121 cccgcgggaa tcaataattc cggagaatgc attttaattt tccatcatgt gcagtatcga
      181 cgaacctgtt ccaggacttt ccacgatttc acttcccgtc cgagcacatg ggatatgaaa
      241 cccgtgagca ggcagcgtga ttccgtagct gacgagtcgg tcagggtgac cagtttgatt
      301 ttatcgatgt ccatgtcttt tcccaaaagc agggtgcgca aggtccctgc ggaaatcggc
      361 tgctcatttt tttgcgttct cgcgcaggcg ctgcaggtga tgccgccgcg gccggcgttg
      421 aaaaaatagg cattgccgtt ggtcaccggc gtcctgcaaa gaacgcagtg atcgagcgcc
      481 ggttcgaaac cggccagttt cagcagacgg atttcaaaaa aacggacaat cgaatcggag
      541 accttttccc tgcccagcag gacgagaaaa tcctgaagca atcggaatat atcttcattc
      601 tttttccctt ccgggctgaa gtggtcggtg agatcgatga agtaagacgc gataagcgtt
      661 ttctccatat tggcgcggat ggcgtcgtag tggtcgacga tgtcgcacga ttcgatgaac
      721 gcgagggtat cgcggctttt tcgggtaaaa atgatatcgg tcagggaaaa cggttcaaag
      781 acgttggcaa atcttttttt gcttctcttg gcccctttgg cgattccctt gagcttgccg
      841 aagtcatgac tgtagaagct gacaattaaa tccgattcgc tgtaactcag ggtgcgcagg
      901 acaaacccgg tagttttaaa atgttcccta acggccgtca tacttgattt caccgccgac
      961 gatggtcatg agggctttgc cctgcatctg acgtccgtgg aaaggagaat ttttgccccg
     1021 cgaacggaac acctgcacat ccactgtcca cggcagcagg ggatcaatga ccgtgatgtc
     1081 tgcatcggcg ccttctttca gcgttccttt cggcaggttc aggatacgcg ccggccgggt
     1141 gctcatcctg gaaaccagtt ccggccagct caggattttg ctgtggatca ggttcagact
     1201 cagcgacagc gatgtttcca atccgctgat gccgttggcg gcatattcga attccacttc
     1261 tttatcggtg cgcgcgtgcg gagcgtgatc gcaggcaatg acatcaatcg ttccgtcggc
     1321 cagtccttcc ttgatggctt tgacatcttt ttcgcccctt aacgggggat tcactttgaa
     1381 aaacgtatcg tagccctgca gggtctcatc ggttaacgtg aaaaagtgcg gggctgtttc
     1441 cgcggtgacg tttacacctc tttccttggc actgcgaatc aaacggacgg aacctgcggt
     1501 actgacatgc gcgatatgca cggaggttcc ggtatattcg gcaatcaaaa tatcccgcgc
     1561 caccatcact tcttcggcga tggcggggat accgtttaat cctaaaatcg tcgaataata
     1621 actttcattc atggctccgt ttgcggaaag atggatgtct tcacagtgat taataacggg
     1681 taattgcagc gaatgcgcgt attccatggc ccggcgcatc agagcggcat tcatgaccgg
     1741 tttgccgtcg tcggaaagcg caatcacgcc cgcttccttg aggtcccaga attccgtcag
     1801 ctgatccccg tctgaatttc tgctgatggc gccgatggga tagacgttgg cgaggttggc
     1861 ttctgccgcc tttgccttga tgaattccgt aatggagcgg ttatcattga cggggttggt
     1921 attgggcatg caggcgatag acgtaaagcc tccggccacc gcggcggcag cacccgaagc
     1981 cacggtttcc ttgtattcga agcccggttc gcgcaaatgc gtgtgcatgt caatcaggcc
     2041 gggtacaatg atcattccgg ccagatcgat aatctttgtt atcttttccg cgtctttgga
     2101 ttttgtggat ttggaaagat tggcgccgac ccgggcgatt ttcccgtttt ccagaaaaac
     2161 gtccaggcgg gaatctatct tttgcgccgg atcgatgacc cgggcgccgg tcaataagag
     2221 tttcattcgc tacctccggt gagcaggtag agcagcgcca tccgtacggc aacgcctttg
     2281 tttacctgat cgagaattac agagaaagag ggatcgtcgg caatgtcggg ggataactcg
     2341 acaccgcggt tgatcggtcc cggatgcatg acaataacat cttttttcgc cagtttgatg
     2401 ttgttgcggt tcaggcagta gcgctgggaa tattcacgca gagacggaaa gatgttttgc
     2461 ttctgccgct ccagttgaat gcgcagcatc atgaccacat cggcatcggt gatggcgtct
     2521 tccattttat caaagacctt gacccccatt tgctcaatat cgcggggcag catggtggcc
     2581 ggaccggcca caacgacggt ggcgcccatt ttggagagcc cgtagatgtt ggaccggacc
     2641 acccggctgt gttcaatatc gccgatgatg gccactttca atccggggaa atttcctttg
     2701 cgttctttga tggtcatcat gtccagcagg gcctgtgaag gatgttcatg tgcgccgtcg
     2761 ccggcattga tgatcgactg tttcaccagc gacgccagaa gatgcggagc gccggcggcg
     2821 ctgtgacgga taacaatcac gtcaggattc atggcttcca ggttgcgggc tgtgtcgatc
     2881 agcgtttctc ccttcatgac ggagctggtg gacgatgaaa tattgatcgt gtcggcgctt
     2941 aatcgtttgc cggctatttc aaaggatgtt cgggtgcggg tgctggcttc gtaaaacaga
     3001 ttgatgaccg ttttcccgcg cagggtcggc actttcttta ttttgcgcgt cgacacctcc
     3061 agaaaagatc cggccgtttc cagaatcagg ttgatttcat cgaccgataa atcttttatt
     3121 cctaaaatgt cttttcgttc aaatttcata gggggcctta aaaaattctt gtttatgttc
     3181 cttcttcaat gacgacttca tcgacgccgt ttttttccac caggtcgacc cggacggctt
     3241 cccagagcga ggagggcaga tttttgccca cgaaatccgc ccggatgggc agctcgcgat
     3301 gaccgcggtc aatgagaacc gccagttgaa tcatccgggg gcgtccgaaa tcaatcagag
     3361 cgtccatggc cgcgcggatg gttctgccgg tgaagagcac gtcgtccaca aggatgacga
     3421 ttttttcatc cagtgaaaaa tttattttcg tttcgcccag cctcggtttt ttgttggaca
     3481 cggaaatatc gtcgcgataa agggtgacat ccagtatgcc catcgggatc ttgccgtgct
     3541 cgatttcgga cattttcttt tgcagccgtt cggcgagata cacgccgccc gttcttatgc
     3601 cgaccaggac aaggttttcc attcccttgt tcttttccag aatttcatag gcgattctgg
     3661 tcaggcaccg ctcaatgcct tccttatcca tgacggtttt tgtgcttttc gtattcgaca
     3721 tgtgcgctaa ccccttgccc ggttaaaaaa aaagcctttc cccaaaacgg taaaggcttc
     3781 ataatcttca tggatctttg attttctcat caacgtttcc tttttcaatc tctccggatt
     3841 aaattaaaag gctttattgg ggatggttat ataaccaaac cgtttgtgtg tcaagatttt
     3901 aaaataattt cccgaatgtc tgctgatatg acgcaatgac aaacggcgga aagcgccgaa
     3961 accttccgga gaacggccta gtgcagcaat tgacccagcc ggttccagcg aagattgttc
     4021 tcatcgcggt tccaggacca gtaaatgaca aaggctttcc cttcgacgtc tttcaggttg
     4081 acaaatcccc agaaacggct gtccaggctt tcatcgcggt tgtcgcccat gacgaaaatg
     4141 gatttctcgg gaacgatgat cggtccgaaa ttgtcccgcg gctgcaggga ttgcggtaaa
     4201 acgaggctat cgctgaaaat gccgtaggaa tccttgaact cctcgtcatt gatgaatatt
     4261 ttcttgtttt ttatttcaat tttgtcgccg gcaacaccga tcacccgttt gatgaaatct
     4321 ttggaccggt cgttgggata aataaaaacg acaacttcgc ctcttttggg ttcgctgacg
     4381 ggaataatgg ttcttcgtaa cagcgggatt ttcacgccgt agatgaattt attgaccaga
     4441 atatggtcgc cgaccagcag tgtcggcacc atggaacggg acggtatctt ataggcgcat
     4501 atgataaatg ttctgataaa caaagcgatc agaatggcaa tgatgatgga ttcgacatat
     4561 tctttaactt ttgatttggt tttgttttct tccgtcatga tggttacaga ctcctaggaa
     4621 taaatgttgg atagagaaaa ggcaatcatg aacagggata taaatccttt gacggcgttt
     4681 ttcccgatgg tattaaaaat aacactgctg agcgtccaat gatgaatttt tgcttcgcag
     4741 tgcctgatga tttccatcat cagaatgccg gcaaggcctc ccagaaggaa accggcaaaa
     4801 gcacccggcg ctgcccaaaa gggggtcaac aggaaggctc ccgtcaccgc tccgatgatc
     4861 gtcgctttca gggatttcag aggacggtgc tcctgcggag gcgtttcacg gatagtccag
     4921 aaaaattcga gggattcggc aaccacggcg caaattagaa gcagtacaat aatttgccag
     4981 ccgacttgct gaaatccggt caagatcgca tacaacacaa catcaaaaaa aatgacaacg
     5041 gttccgggca aatcgacgaa attcaagaaa atacccgcaa acagtatcag aataaaaatt
     5101 gtaagtcccg ctgccgccca aaatgacaaa ttatggagtc ctcttcattt tcgaaatttg
     5161 aaggttttca aacatcagaa caatcgtaca ggctatgtaa atggaggagt aggttccgat
     5221 gaccacacct accagaagcg cgaaggcgaa atcgcgaatt accgcgccgc cgaaaagata
     5281 cagggcgaga accgtgagaa aaacggtcag tgaggtcaga acggttctgc tgagcgtctg
     5341 attaatgctc agattgatgg tctcgcggag ttgtcttttg ggattcaatt taacattttc
     5401 ccggattctg tccagagtga cgatggtgtc gttgatcgaa aaaccgatga gaaaaaccag
     5461 cgccgccagg atggagatgg taaattccat gttcatgatg gaaacagctc cgatcgtaat
     5521 gatgacgtca tgaaccagcg caacgatgcc tcccacgccg tagcggaatt cgaaacgaaa
     5581 gcctacgtaa atgagcatgg ctatccagga caagacgacc gcaatgacgg ctttacgttt
     5641 gaagtcggcg ccgacctttg acccgacgac ctccacgcgc ctgatttcaa aatctttact
     5701 gaacgcggcg gccagcggct gctcaacgtt agcggccagc accgtttgat ccgaaaaaga
     5761 ttctgacgtg cggattaaaa attcgttggt tccgaactgt tgaatggtgc tgttttgaag
     5821 cctggtcgat ttgagagccc cccggatttg atcggcggaa atattttttg aaaattttac
     5881 ctgaatgacc gagccgccgg caaagtcaat accgggattt aacccgccat gataaataac
     5941 cgatccgata ctgacgatga taagaatgac tgaaaatgtt atcgcgtatt ttatttttcc
     6001 aacgaaatcc agattgattc ccggttttat caactgcata tcatgtctcc tgcctgcctg
     6061 tatattttta aatactcagc tttttaatgt taaagttcgt ggtgatataa tcgaaaatta
     6121 ccctggtgac gaaaactgcc gtaaaaagac tggccagcag gccgatgatg gttgttacgg
     6181 caaatccctt gatcgggcct gttccgaaca tgatcagaaa agcagcggcg actattcccg
     6241 tgatgtgggt gtcaataatc gtaatgaagg cccgattata ccctgtatcc agtgcgattc
     6301 tgatggtctt gcccgtgcgc agttcttcac gaatccgttc aaagatcagg atattggcgt
     6361 ccaccgcgac acccaccgtc aaaagcagtc cggcaatgcc cggcaatgtc agtgtggccc
     6421 tgaaggccgc aagaatggcc agtacaagaa aaatgtttgt gatcagggca acgtcggcga
     6481 ttgcgccgga aaggcgataa tagatgatca gaaataaaat gaccaaaagc gatccgatca
     6541 tggtggccat gacgccttgc cggatggaat cgctgccgag cgaaggaccg accgttcttt
     6601 cctccagaat attgacgggc gcgggaagag caccggcccg caaaacaatg gcgagatcgc
     6661 gcgcttcttc catggtaaag ttcccgg
//
LOCUS       DAOOLB010000099         6649 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_13723_length_6649_cov_47.840461, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000099 DAOOLB010000000
VERSION     DAOOLB010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13723_length_6649_cov_47.840461"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /gene="dnaB"
                     /locus_tag="PLF77_09665"
     CDS             complement(<1..1343)
                     /gene="dnaB"
                     /locus_tag="PLF77_09665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPC09162.1"
                     /translation="MKEKDPSQYKLPPQNLEAEQSVLGSILLENSATNRVFEILSERD
                     FYSEAHRKIFIVIKDLSEKNEPVDLITLSNALKDKSLLDSVGGTTYLASLVDNVPSAA
                     NAANYAKIVKEKAILRGLITSATEIIDNCYETGSDVDTILDKAEHSIFEISENKVRQD
                     YFPIRDIIKDSFRSIEDLYSRQQLITGVPTGFGKIDDLTAGLQKSELIIIAGRPSMGK
                     TAFALNIALHAAMEAQIPTAVFSLEMSKEQLAFRLLSSEARVDAQRLRKGFLGETDWP
                     KLTTAAGRLSDAPLYIDDTPAITVLEMKAKSRRLKADKGLGLVVVDYIQLMRSSYSHD
                     SREREISDISRSLKALAKELNVPVVALSQLNRQVESRTNRRPQMADLRESGAIEQDAD
                     VIAFIYRDEVYNKSEDNIDKGIAEIIIAKQRNGPTDTVKLAFVDKYTSFENLSRV"
     gene            complement(1343..1795)
                     /gene="rplI"
                     /locus_tag="PLF77_09670"
     CDS             complement(1343..1795)
                     /gene="rplI"
                     /locus_tag="PLF77_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPC09163.1"
                     /translation="MKVILKQSVRSLGKAGTVVKVNDGYARNFLIPKGLAIEADEKNI
                     RLFEHEKNNILKKAAKEHKNAQDLADVLSRETITIARKVGDQDKLFGSVSAKDIEAAL
                     KEKGYDINRKMIMHEHGEHIKELGEFAVKIKLAHDVEAQITLKVVGES"
     gene            complement(1840..2817)
                     /locus_tag="PLF77_09675"
     CDS             complement(1840..2817)
                     /locus_tag="PLF77_09675"
                     /inference="COORDINATES: protein motif:HMM:NF021506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HPC09164.1"
                     /translation="MSSFSFFKKSGAPASGNSFSGLLSAVGLFAFAAVFVPLVGPVFL
                     FLLPMIAFVNGATNGIFKTALCLLFSLAVLMLLSVGLNLDVPAVAIFTMGMAGLLMAH
                     MAAENYSIEKIVTGPALLIFGAIGFYFVYDAVGLGVNPWLLVKNFITVTISETIKFYS
                     QMPLKPDDISMIKDNENNIINSLIHIFPSLMVILSVFIIWANLLLGKTYLRRFGIVYA
                     QMAILARWKVTDWAIWIFIISGLLLFIPQKNFNFIGLNVFLIVCLVYLLQGLAIVSFL
                     FQNKNVPFLIRYIFYFLIAVQQILMIPIAVIGLFDVWFDFRKRFQKNHL"
     gene            complement(2987..3256)
                     /gene="rpsR"
                     /locus_tag="PLF77_09680"
     CDS             complement(2987..3256)
                     /gene="rpsR"
                     /locus_tag="PLF77_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPC09165.1"
                     /translation="METQNGEKQGRYPQKKFFHRKKFCRFCSDSNIKIDYKNTAILNN
                     FVTERGKIMPRRITGNCASHQRELALAIKRARQIAIMPYVTADGR"
     gene            complement(3266..3742)
                     /gene="rpsF"
                     /locus_tag="PLF77_09685"
     CDS             complement(3266..3742)
                     /gene="rpsF"
                     /locus_tag="PLF77_09685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPC09166.1"
                     /translation="MQRYEVVAIVLADLIEDEINALIERSQTIITDRKGVIAKVDKWG
                     KRHLAYEIKKQRDGYYFLIDFAGNGSIVNEIERNYKIDDRVIKFMTVKKEGATTAEGI
                     QQEAAAAEAKRVQAKVEAEAAAAEGKQINQIEKAPRESRPRVRKDHNKEETQTKGE"
     gene            complement(3992..4912)
                     /locus_tag="PLF77_09690"
     CDS             complement(3992..4912)
                     /locus_tag="PLF77_09690"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPC09167.1"
                     /translation="MGKKVGLVLGGGGVRGFSHIGVLKVLEEEGIDIDLIVGTSAGAL
                     IGGAYASGQAPDEIESKIDAYLQSPEFDDSRMKSIGLSFTQEEKNILKKAQQFIMNRV
                     LFVQAFFKPSILPSHDFLSLMNYFLPDLDIKETRIPLHVVSTDLISGKKIVFSEGSLR
                     KVVLASCAVPGAVEPVRYGDWLLADGGITSLVPVHAAREAGADVVIAVTVDRDLQTNL
                     RIETAKDVVFRAGEITPSVLEAAELADADVIIRPQVGNLHWMDFKRAVDLVKAGESAA
                     RDSLKSIHASLPIYRRITRLAGKLFKQSRL"
     gene            complement(4948..6618)
                     /locus_tag="PLF77_09695"
     CDS             complement(4948..6618)
                     /locus_tag="PLF77_09695"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HPC09168.1"
                     /translation="MHGMLTDIGISVLAATALGFVFQLVRQPVILGYLVAGALIGPAM
                     GFNLVSEAASIEVISEIGLILLLFIIGLELNPSKLLASGRQLIYAGIGQFIFCVLIGL
                     GFFLLLGYELSRNNIDALYLALFCALSSTAIVVKLLYDKFELDTFPGRITLGILIFQD
                     LWAILILALQPHFTNPQFLLVFIALGKSVALLAIGFLLSKYLLSWIFKKISQTPEMVV
                     AMSMAWCALMAGIGSGIGLSMEMGALIAGVAISTFPYSMHVTAKVLPLRDFFLTLFFL
                     SIGMKIPFPDAAILGMALIIVLFVIVSRFLTIYPLLTLAGSGRRTSFIASLNLAQISE
                     FSLVVAALGVHYGHIDQRMMSLIIYAMAMTSVLSSYFIKGNHAAYLVFNRFLEKRGFP
                     ARTKENHDEEEKNVYPIVILGFHRGAREFIGRLETETPDLLKKLLVIDFNLENLKELS
                     NKNIKGIFGDIGSMDTLEHAHITAAQIIFSTIPDMLLRGTSNKALVTSCRALAPSAYI
                     VATADSADQIDVLRKSGADEVLLPYVLIGEKLTRFVEQTCLTAESCKL"
BASE COUNT         1782 a         1678 c         1530 g         1659 t
ORIGIN      
        1 tccacccgtg acagattttc gaaacttgtg tatttgtcca caaaggccag cttgacggtg
       61 tcggtggggc cgtttctctg tttggcaatg atgatttccg caataccttt gtcgatattg
      121 tcctccgatt tgttgtacac ttcatcccgg taaataaagg cgatgacgtc ggcatcctgt
      181 tcaatggcgc ctgactcgcg caaatccgcc atctgcggcc ggcggttggt gcggctttcg
      241 acctgacggt tcagctgtga aagcgccaca accggcacat tgagttcttt ggccagcgcc
      301 ttcagggacc ggctgatatc cgatatctcc cgttcccgtg aatcgtgact gtaactggag
      361 cgcatcagct gaatgtaatc cacaacaacc agtcccaagc ccttgtccgc tttgagcctc
      421 cgggacttgg ccttcatttc tagaacggta atagcgggcg tgtcatcgat gtaaagaggc
      481 gcgtccgaaa ggcggccggc ggcagtcgtc agtttcggcc agtctgtttc tcccagaaaa
      541 ccttttcgta agcgctgggc atccaccctg gcctcagagg acagaagacg aaaagcaagc
      601 tgttccttgg acatttccag cgaaaagacg gcggtcggaa tctgcgcttc cattgcggcg
      661 tgcagtgcga tattcagggc aaacgccgtc ttgcccatgc tgggacgtcc tgcaataata
      721 atcagttcgg atttctgcaa gccggcggtc aaatcatcaa tctttccgaa gccggtcggc
      781 acgccggtaa tcaactgctg gcgggaatac aaatcttcga tagagcggaa actgtcttta
      841 ataatatccc ggatcggaaa ataatcctgg cgcaccttgt tttcggaaat ttcgaaaatg
      901 ctgtgttcgg ccttatccag aatagtatcc acatcggaac cggtttcgta acagttgtcg
      961 ataatttccg ttgccgacgt gatcagaccg cgtaaaatcg ccttctcttt gacaattttc
     1021 gcataattgg cggcattggc cgccgatgga acgttgtcca ccagagaagc caggtaggtt
     1081 gtaccgccga cagaatccag aaggctttta tctttgaggg cattgctcag ggtaatcaga
     1141 tcaaccggtt cgtttttttc ggacagatcc ttgatcacaa tgaaaatctt ccgatgagcc
     1201 tcggagtaga aatccctctc ggataaaatt tcgaagaccc tgttggtggc actgttctcc
     1261 agcagaatac tgcccaggac agactgttcg gcctcgagat tctgcggcgg gagtttatat
     1321 tgcgatggat ctttttcttt cattatgatt cgccgacaac cttcaacgtg atctgtgcct
     1381 caacatcatg agccagtttt attttgaccg cgaactcgcc gagttcttta atgtgctcgc
     1441 cgtgctcatg catgatcatc ttccggttaa tgtcgtatcc tttttctttt aacgcggcct
     1501 cgatatcctt tgcgcttacc gaaccgaaaa gtttgtcctg atcaccgacc ttgcgggcaa
     1561 tggtaatggt ttcgcgggac agaacatcag ccagatcctg cgcgttctta tgttcttttg
     1621 cggccttctt cagaatattg tttttttcat gttcgaagag tctgatgttt ttttcatcgg
     1681 cttcaatcgc cagtcccttg ggaattaaaa aattacgggc gtacccgtca ttgactttca
     1741 ccaccgttcc ggctttgccc agggaacgca cactttgttt cagaatgacc ttcatgaaaa
     1801 tcctccgttt ttataaaaat agtattgcga tcattatgtt cataaatgat ttttttgaaa
     1861 acgcttgcgg aaatcaaacc atacatcaaa cagcccgatg accgcaatag gtatcataag
     1921 aatttgctgc actgcaatta aaaaataaaa aatatagcgg attaaaaaag gtacgttttt
     1981 gttctggaag agaaagctga caatcgccag cccttgcagc agatacacca agcagacgat
     2041 cagaaaaaca tttaacccaa tgaaattaaa gttcttttgt ggaatgaaca atagcaggcc
     2101 ggatatgata aaaatccata tggcccagtc ggtcactttc caacgcgcca gtattgccat
     2161 ttgcgcataa acgatgccga atcgtctcag ataagttttg cccagtaaaa gattagccca
     2221 gatgatgaat acggacagaa tgaccatcag ggacgggaaa atgtggatga ggctgttgat
     2281 gatattgttt tcattatctt tgatcatgct gatatcgtcc ggttttaaag gcatctggct
     2341 gtaaaatttg attgtttcgc tgatggtcac ggttataaaa ttcttcacca gcagccaggg
     2401 attcaccccc aggcccacag catcgtaaac gaaataaaaa ccaatcgctc caaaaatcaa
     2461 taaagccggt cccgtaacta ttttttcgat ggagtaattt tcagccgcca tgtgcgccat
     2521 aagcaatccg gccattccca tggtaaaaat cgcgacagcc ggcacatcca gattcagccc
     2581 gacggataaa agcataagaa ccgctaaaga aaataaaaga cacagtgctg ttttaaagat
     2641 accgttggtt gctccattga cgaaggcaat catcggaagc agaaaaagaa aaacggggcc
     2701 gacaagcgga acaaaaacgg cggcaaaagc aaacagacca acagcagaca ggagaccgga
     2761 aaaagaattg ccggacgcgg gtgctccgga ttttttaaag aaagaaaaac tgctcacgat
     2821 gcaacacccc gttaaccgtc gctttttctg aaaaagaagg taacgccgcc cgcggcgtta
     2881 cctgactgaa atgttgttga attgatttta aaccgaaacg aatgactcag ttttgaaaaa
     2941 taatcatcac accattattc taacgacaaa aagctgcgga acagatttat ctaccgtcag
     3001 ccgtcacata aggcataatc gcaatctgcc gggcgcgttt aatggccagt gccagttccc
     3061 gctgatggga agcgcagttg ccggtgatgc ggcgcggcat gattttgccc cgttccgtta
     3121 cgaaattatt aagaatcgca gtatttttat aatcaatctt aatattggaa tccgagcaga
     3181 acctgcagaa tttttttctg tgaaaaaact tcttctgggg atatcttcct tgtttttctc
     3241 cgttttgtgt ttccattgcc atggcttatt cccccttcgt ttgagtttct tctttgttgt
     3301 gatcttttct aacgcgcggt ctggattccc gcggcgcctt ctcaatttga ttaatctgtt
     3361 tgccttcggc agcagcggct tccgcctcta ccttggcctg aacgcgcttt gcttcggctg
     3421 cggcggcttc ctgctgaata ccttcggcag tggtcgcacc ttccttcttc accgtcatga
     3481 atttaatgac ccgatcatcg atcttgtaat ttctttcaat ctcgttgaca atggaaccgt
     3541 tgccggcaaa atcgatgaga aaataataac cgtctctctg ttttttgatt tcataggcga
     3601 gatgccgttt gccccacttg tcaaccttgg caatgacacc tttgcggtcg gtaatgatgg
     3661 tctgacttct ttcgattaac gcgttgatct cgtcctcaat taaatcggcc aggacgatag
     3721 ccacaacttc atatctttgc aatgttttct cctttcggct ctaaagcccg attggacggg
     3781 caaggagtcc taaaaatagt aggcgcgtta tctataccaa accaaaacag aaatcaagaa
     3841 ctaaatgacc ttaataaaat gatgttcaaa atcagataat taaatgataa tctccgtcct
     3901 tcctttaagc tggatcaagc acattgcctg tccaagataa ttgaaaaacc gttccggttc
     3961 aatctaaaaa aacgtaaacc ggaaatcggg tttagagcct tgattgttta aataattttc
     4021 cagccaagcg tgtaatgcgc cgataaattg gcagagaagc gtgaatgctt ttcagggaat
     4081 cgcgggcggc gctttctccc gccttgacaa ggtccacggc ccgtttgaaa tccatccagt
     4141 gaagattgcc gacctgggga cggatgatca cgtcggcatc cgccaattcc gccgcctcca
     4201 gaacgctggg ggtgatttct cccgccctga aaacgacgtc cttcgccgtt tcgatcctga
     4261 gattcgtttg caaatctcga tccaccgtca cagcgatgac gacgtcggct ccggcctccc
     4321 gtgcggcgtg aaccggcacc agactggtaa tgccgccgtc agccagcagc cagtctccgt
     4381 aacgaaccgg ctcgacggct cccggaaccg cacaactggc cagcacaacc ttgcgcaacg
     4441 atccttcgga aaaaacgatt tttttaccgg agatcagatc ggtggaaacc acgtgaagag
     4501 gaattcgtgt ttctttaata tcgagatccg gcagaaaata gttcatcaga gagaggaagt
     4561 catgggaagg cagaatggac ggtttgaaaa aggcctgaac aaaaagaacg cgattcatga
     4621 taaattgttg tgctttcttg agaatgtttt tctcctcttg tgtaaaagac agtccaatgg
     4681 atttcatcct ggagtcgtcg aactcaggac tttgcagata agcgtcgatt ttgctctcga
     4741 tctcatcggg agcctggccg gaagcataag cgcctccgat caaagcaccg gcgctggttc
     4801 ctacaatcag atcaatgtcg atgccttctt cttccagaac cttgagaacg ccgatatgag
     4861 aaaaaccgcg gacgccccct ccgcccagta ccagtccaac ttttttaccc atccatttca
     4921 taaacagata tctacaccgg ttacgtttta gagtttgcag gactccgccg tcaggcaggt
     4981 ctgctcaaca aacctcgtca gtttttctcc aatcaggaca taaggcagca gcacctcatc
     5041 cgccccgctt ttcctcaaga catcaatctg atcggccgaa tcagccgtag caacgatgta
     5101 ggcgctgggc gccaaggcac ggcatgaagt caccagagcc ttgttgctgg ttccccggag
     5161 gagcatgtcg ggaatcgttg aaaaaattat ttgtgctgcg gttatatgag cgtgctccag
     5221 cgtatccatg ctgccgatat cgccgaaaat cccttttatg tttttattgc tcaattcttt
     5281 taaattttca agattgaaat caatcaccag aagctttttc aataaatccg gggtctccgt
     5341 ctccagccgg ccgataaatt cccgcgcgcc gcggtggaat cccagaatca cgatggggta
     5401 gacatttttt tcctcttcat catgattttc tttcgtcctg gccggaaatc cccttttctc
     5461 cagaaaacgg ttgaatacca gatacgccgc gtgattcccc ttgataaaat atgaagaaag
     5521 aacagacgtc atggccatgg cgtagataat caacgacatc atccgctgat caatgtgccc
     5581 gtaatgcacg ccgagcgcgg ccaccaccag agaaaattcg ctgatctggg cgagattcag
     5641 actggcgatg aaactggtcc gcctgccgct gcccgccagc gtcagcagcg gataaatcgt
     5701 caaaaaacga gaaacaatca caaataggac aatgatcaac gccatgccca gaatcgcggc
     5761 atcgggaaaa ggaattttca taccaatgga cagaaagaaa agggtcagaa aaaagtcgcg
     5821 caggggaaga actttcgccg tcacatgcat gctataggga aacgtcgaaa tcgcgacacc
     5881 ggcaatcaga gcgcccattt ccatcgaaag accgatccct gaaccgatac ccgccatcaa
     5941 agcgcaccac gccatagaca tggccaccac catttccggc gtttgtgata tttttttaaa
     6001 aatccagctc aacaggtact tgctgagcaa aaaaccgatg gccagcagag ccacgctctt
     6061 gccgagagcg ataaaaacca gaagaaattg cggattcgtg aaatgcggct gaagcgccag
     6121 aatcagaatg gcccacaggt cctgaaaaat aagaatgccc agcgttatcc gtccggggaa
     6181 ggtgtccagt tcaaacttgt cataaagaag tttaacgaca atcgccgtcg aactcagagc
     6241 gcaaaacagg gccagataaa gggcgtcaat attgtttcgg ctcaattcgt aaccaagcag
     6301 cagaaaaaat cccagcccga tcagcacgca gaaaataaac tggccgatac ccgcatagat
     6361 cagctgacga ccggaagcca ggagtttgga cggattgagt tccagaccga tgataaaaag
     6421 cagcaggatc aggccgatct cggagatgac ctcgatgctg gcggcttcgg aaaccagatt
     6481 gaatcccatc gccggcccga tcagcgcccc ggcgaccaga taaccgagaa tgacgggctg
     6541 acggacaagc tgaaatacaa agcccagagc ggtcgccgcc agaacagaaa tgccgatgtc
     6601 ggtaagcatg ccatgcataa gttcgtatct ttaatgcgtc actgcgtat
//
LOCUS       DAOOLB010000100         6625 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_13797_length_6625_cov_44.713394, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000100 DAOOLB010000000
VERSION     DAOOLB010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6625
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13797_length_6625_cov_44.713394"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1446
                     /locus_tag="PLF77_09700"
     CDS             169..1446
                     /locus_tag="PLF77_09700"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPC09169.1"
                     /translation="MSFEKFELDAKILAGVQACGYNVPTPIQLQSIPPIMEGRDVMGL
                     AQTGTGKTAAFVLPILQRLMQGARGHVRALIIAPTRELSEQTHTAIMQLGRKTKLRSV
                     SIYGGVNINGQINKLRNNVEIISACPGRLLDHINRGTVDLSRIEVLVLDEADHMFDMG
                     FLPDIRKIVKHLPAQRQTLLFSATMPDDIRSLANDLLINPVNIKIGHTAPVETVSHKF
                     YPVQMSSKTDLLKDILDKTDMDSVLIFTRTKSRAKSVALQLGKSGYKVTSLHGNLTQQ
                     KRQHALDGFRDGRYEIMVATDIAARGIDVNSISHVINYDMPSTTDAYTHRIGRTGRAA
                     KTGDAFTFVTGEDRGLAQSLERVLGKKLHYQNVDGLTMSLPVDDRVSASRNSRGRNMK
                     PHNDRKKFSPKARANSRLNRRSFDSSNRSQWAH"
     gene            complement(1539..1994)
                     /locus_tag="PLF77_09705"
     CDS             complement(1539..1994)
                     /locus_tag="PLF77_09705"
                     /inference="COORDINATES: protein motif:HMM:NF024304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPC09170.1"
                     /translation="MRKKERAIKDRKDIDGIIRRCRVCRLAMSDEDQPYIIPLNFGYD
                     GFCLYFHAAPEGRKIDILKRNNRVGFEFDILHEILDAAQPCAWSAKYESVIGSGTAEI
                     INDVKGKKAALECIMRQYGSDAGDFSEKVMKKTLIIRVRIREISGKARR"
     gene            complement(2039..2878)
                     /locus_tag="PLF77_09710"
     CDS             complement(2039..2878)
                     /locus_tag="PLF77_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HPC09171.1"
                     /translation="MTKHLFYPVKASIKKSYIHGYDPVENIRLQDQASTLVELLHVDT
                     AYAPGSTVLEAGCGVGAQTITLAKNSPGALITSIDISAASIDEARRKTEAAGIQNVHF
                     EQADIFNLSFASQSFDHIFLCFVLEHLPQPVEALIILKRYLKKNGTVTVIEGDHGSAY
                     FYPDSAAAHKAIESQVELQRRAGGNANIGRALHPLLNQAGFRSVCVSPRMVYVDSGKP
                     QLVDGFIKKTFTAMIEGVRAEAIDAGIIGKNVFDQGIRDLYRTAEPDGLFCYTFFKAT
                     GYK"
     gene            complement(2900..3766)
                     /locus_tag="PLF77_09715"
     CDS             complement(2900..3766)
                     /locus_tag="PLF77_09715"
                     /inference="COORDINATES: protein motif:HMM:NF016143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HPC09172.1"
                     /translation="MRWRGERQSDNVEDRRGMSISRGAKVGGIGGLGLVAIVVISMFM
                     GVDPSSILQVVEQNTQSSSISVEQSATTAANDNARNFVAVVLAETEDVWNDVFQQAGR
                     TYEEPKLVLFSEAVESACGMAGSATGPFYCPADHKVYLDLAFFEELHSRFGASGDFAQ
                     AYVIAHEIGHHVQTQLGIIQKVHELQSRTSKSEANKLNVKLELQADCLAGMWAHQADK
                     KRNILEAGDIEEGLNAASAVGDDRIQKQTQGYVVPDGFTHGSSAQRVTWFKRGFEQGN
                     IGACDTFNAARL"
     gene            complement(4035..4553)
                     /locus_tag="PLF77_09720"
     CDS             complement(4035..4553)
                     /locus_tag="PLF77_09720"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPC09173.1"
                     /translation="MVKLYRCIICGDAYIGASPPANCPFCGAHMEYIMEAKDAVVNFD
                     VPLSAKDRANAEHALKVEISNATFYSCAAGKTDDPEGKVLFKALGKIEAEHASIWKKI
                     LKLASLPPGNETCHTENVGNLKESHARETRAIEFYRKSAAEADHPRIRQLFEALVEIE
                     TDHLFLSEERLH"
     gene            complement(4589..4963)
                     /locus_tag="PLF77_09725"
     CDS             complement(4589..4963)
                     /locus_tag="PLF77_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HPC09174.1"
                     /translation="MANRNEVYKCNVCGNIVEILHAGVGKLVCCDQPMKLMTENTTDA
                     AKEKHVPVVEKVAGGFKVTVGSVAHPMEEKHYIEWIEVIADGKIYRQYLAPGQAPEAV
                     FPIEAISITAREYCNLHGLWKA"
     gene            complement(4989..5486)
                     /locus_tag="PLF77_09730"
     CDS             complement(4989..5486)
                     /locus_tag="PLF77_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HPC09175.1"
                     /translation="MSKTEEALKEAFAGESQANRKYLAFAAKADQEGFAQAAKLFRAA
                     AEAETVHAHAHLRAMKGIRSTKENLQEAIAGETHEFKSMYPAMIEAAKAESHKEAERS
                     FNFANEVEKIHAALYQKALDHLDGSQETYSYYVCPVCGYTVENEAPETCPVCGAKGKM
                     FKKVD"
     gene            complement(5607..>6625)
                     /locus_tag="PLF77_09735"
     CDS             complement(5607..>6625)
                     /locus_tag="PLF77_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09176.1"
                     /translation="KEFADAFARAWFKLTHRDMGPRSRYLGPEVPKEVLIWQDPIPAV
                     NHKLINKADANSLKSKILASGLSISELVSTAWASASTFRGSDMRGGANGARIRLAPQK
                     NWEVNEPERLAGVLKTLERIQKAFNKDQKGDKKVSLADLIVLAGCAGVEQAAKNAGYK
                     ITVPFTPGRMDASQKQTDVASFGVLEPKADGFRNYQKAKYTVLPEEMLVDKAQLLTLT
                     APEMTVLIGGLRLLGANFGGSPHGVFTSRPGTLTNDFFVNLLDMGTEWKVSKDADVFE
                     GRDRKTGELKWTGTRVDLIFGSNSQLRAVAEVYACKDSQEKFLDDFVAAWNKVMNLDR
                     FDLA"
BASE COUNT         1517 a         1754 c         1632 g         1722 t
ORIGIN      
        1 tcggcaggaa aatccggcta atcgtaaccg aatccaactt tagcgccagg tttgcagcct
       61 gttttgggct tgtcatcatg gcgcacggcg atgtgttttt aaacaaccgc gacgaataga
      121 cctgggttca aatgtcacaa atactgaaaa aagaaggaaa aagaaaaaat gagttttgag
      181 aagttcgagt tagatgcgaa aatactggcc ggcgtgcagg cgtgcggcta caatgtgccg
      241 acacccatcc agttacaatc tatcccgccg attatggaag gccgcgatgt gatgggcctg
      301 gcccagaccg gtacgggaaa aacagcggct tttgtgctgc cgattttgca gcggctgatg
      361 cagggggcac gcggtcatgt ccgcgcgctg attattgccc cgactcgcga gttgagcgag
      421 caaacccata cggccattat gcaactggga cgaaaaacca aattgcgtag tgtctccatt
      481 tacggcggcg tgaatatcaa cggccagatc aacaaactgc ggaataatgt ggaaatcatc
      541 tccgcctgtc ccggtcgtct gctggaccac atcaaccgcg gcaccgtgga tttatcccgg
      601 atagaagtgc tggttctgga tgaagccgac catatgtttg atatgggctt cctgccggat
      661 attagaaaaa tcgtcaaaca tcttcccgcg cagcgtcaga ccttgctgtt ttccgcaacc
      721 atgccggacg acatccggtc gctggccaac gatctcttga tcaatccggt aaatattaaa
      781 atcggccaca cggcaccggt cgaaaccgtg tcgcataaat tttatccggt gcagatgagc
      841 tctaaaacgg atctgctgaa agatattctt gataaaacag atatggattc ggttctgatt
      901 tttacgcgaa ccaaatcgcg cgcgaaaagc gtggccctgc aactgggaaa atcgggatac
      961 aaagtaacgt cactgcacgg caatttgacc cagcagaaac gtcagcatgc gctcgacggc
     1021 ttccgcgacg gccgttatga aatcatggtc gccaccgaca ttgccgcccg cggcattgat
     1081 gtgaacagca tttcccatgt gatcaattat gatatgccct caacgaccga tgcctacaca
     1141 catcgtatcg gccggacggg acgcgctgcc aaaaccggcg atgcctttac ctttgtaacc
     1201 ggagaggatc gcggcctggc ccaatcgctg gaaagggtgc ttggcaaaaa gctgcactac
     1261 cagaatgttg acggattaac aatgagcttg cccgttgacg atagagtttc cgccagccgt
     1321 aatagcaggg gaaggaacat gaagcctcat aacgatagga agaagttttc tcccaaagcg
     1381 cgggccaaca gcagattgaa tcggcgttct ttcgattctt ccaaccgcag tcagtgggcg
     1441 cattaattca atcaaaaaac accatcgggt gatcactctt taaaatattt attttagaag
     1501 ttatcacccg atggtgaaag actgttaatt ttcgttctct atcgtcgggc tttcccgctg
     1561 atttcccgta tgcgcacacg aataatcaga gtttttttca ttaccttttc cgaaaaatct
     1621 ccggcatcac ttccatactg acgcataatg cattccagcg cggctttttt gcccttcacg
     1681 tcgtttatga tttctgccgt gcctgaacca atcacacttt cgtattttgc actccatgcg
     1741 cagggctgtg ctgcatcaag gatttcatga aggatatcaa attcgaatcc gacgcgatta
     1801 tttcttttca ggatatcgat ttttcttcct tcgggtgcgg catgaaaata gaggcaaaag
     1861 ccgtcatagc cgaagttgag gggaataata taaggttgat cctcgtcgga catggccaga
     1921 cggcacaccc gacaacgacg gatgatcccg tcaatatctt tcctgtcctt tattgctctt
     1981 tctttttttc gcattttcaa tctgacctga tcggggccga tgatgatttg tccggcaatt
     2041 atttgtagcc ggtggcttta aaaaacgtat agcagaagag gccgtccggt tcggcagtgc
     2101 gatataaatc gcggataccc tgatcgaaaa cgtttttccc gatgatcccc gcgtcgattg
     2161 cctctgcacg cacgccctca atcatggcgg tgaacgtttt tttgatgaat ccgtccacta
     2221 gttgcggctt gccggaatcc acataaacca tgcggggaga tacgcaaaca gatcggaaac
     2281 ccgcctgatt cagcaacgga tgcaatgctc tgccgatgtt ggcgttacct ccggcgcgcc
     2341 gctgcagttc cacctgactc tcaatggcct tgtgcgctgc cgcgctgtcc ggataaaaat
     2401 acgccgagcc gtggtctcct tcaataacgg tgaccgtacc gtttttcttt aagtaccttt
     2461 taaggatgat caacgcttca acgggctgcg gcagatgttc aagaacaaaa cagagaaaga
     2521 tgtgatcaaa cgattgcgat gcaaaggata aattaaaaat atcggcctgt tcgaaatgaa
     2581 cattttgaat gccggccgct tcggtttttc ttctggcttc atcaatggag gccgcggaaa
     2641 tgtcgatcga agtgatcaac gcgccgggac tgtttttcgc cagggtgatg gtctgtgcgc
     2701 cgacgccgca accggcttcc agcaccgtac tgcccggcgc ataagcggtg tcgacatgca
     2761 gcaattctac cagagtggag gcttgatcct gaagtcttat gttctctacc ggatcataac
     2821 cgtgaatgta tgattttttt atgctcgctt ttacaggata aaaaagatgt ttcgtcatgt
     2881 ccgcccggtt aatcatcctt tacaggcgag cggcattaaa ggtgtcgcag gcgccgatat
     2941 tgccctgttc gaagccgcgt ttaaaccagg tgacgcgctg cgcggaactg ccgtgcgtaa
     3001 agccgtccgg caccacgtaa ccctgcgtct gtttctggat gcggtcatcc ccgacggcgc
     3061 tggcggcatt caatccttct tcaatatcgc cggcttccag aatatttctt ttcttgtcgg
     3121 cctgatgcgc ccacatgccc gcgaggcaat ccgcctgcaa ctccagctta acgtttaatt
     3181 tattcgcttc ggatttgctt gtgcgcgact ggagctcatg cactttctgg ataatgccca
     3241 actgggtctg aacatgatgg cctatttcat gggcgatgac gtacgcctgg gcgaaatcac
     3301 cggatgcgcc gaaacgtgaa tgcagctctt cgaaaaatgc caggtcgagg taaaccttgt
     3361 gatcggccgg acaataaaaa ggtcctgtgg ccgatccggc catgccgcat gcggattcaa
     3421 cggcctcgga aaacaataca agtttcggtt cttcataggt gcgtcccgcc tgctgaaaaa
     3481 catcgttcca gacatcctcc gtttccgcca gcaccaccgc gacaaaattg cgcgcattgt
     3541 cattggcggc tgtggttgcg ctttgttcaa cagaaattga agaagactgc gtattctgct
     3601 caacgacctg caatattgaa ctcggatcaa cgcccatgaa catgctgatg accacgatgg
     3661 ccaccagacc gagtccgccg atgccgccga cctttgcgcc gcgggaaatc gacattcctc
     3721 ggcggtcttc cacgttgtca ctttgtcttt cgcccctcca gcgcattgtt ttctcctttt
     3781 tagggttaca tcaggaataa tatgattacg gcccggacgg ccggaatcga cccttatgct
     3841 tcagttgtca gtatagaaac aaggttttcg gctgtcaaga taattcatct tcgttgattt
     3901 tattggagaa aatagtcatc aatcaatccg atgcggacat gattatgctt taagaaatgt
     3961 atccggattt gttcagtaag ggggtgacgc gcagaccgcg gaaatacgac cgcgatctgc
     4021 acgcagcctt tgttctaatg cagtctttct tcggacaaaa acaggtgatc tgtttcgatt
     4081 tccaccagcg cttcaaaaag ctgccggatc cgcggatgat ccgcttcggc ggctgacttt
     4141 ctgtagaatt cgatagcccg tgtttccctt gcgtgggact ctttcagatt tccaacattc
     4201 tccgtatggc aggtctcgtt tcccggagga agggatgcca gtttgagaat ctttttccaa
     4261 atggaggcat gttccgcttc aattttgccc agcgccttga acaaaacctt tccttcggga
     4321 tcatccgttt tgcctgcggc gcatgaataa aatgtcgcgt tgctaatttc caccttgagc
     4381 gcatgttcgg cattggcccg atcttttgcg ctcagaggca catcaaaatt cacgaccgca
     4441 tcttttgcct ccataatata ttccatgtga gcgccgcaga acgggcagtt cgccggagga
     4501 ctggcgccga tatacgcgtc gccgcatatt atgcatcggt atagtttaac catgtgttct
     4561 ccttttaaga taatgatgca gcgttaactt atgctttcca caagccgtga agattgcagt
     4621 attcgcgcgc tgtaatgctg attgcttcga tggggaaaac cgcctcgggc gcctggccgg
     4681 gcgctaaata ctggcggtaa atttttccgt ctgcgataac ttcaatccat tcaatgtagt
     4741 gcttttcttc catgggatgc gccacgctgc caacggtcac cttgaaaccg ccggccactt
     4801 tttccaccac ggggacatgt ttttccttgg ctgcatccgt cgtgttttcc gtcatgagtt
     4861 tcatgggctg atcgcaacac acaagctttc ccacgcccgc gtgaagtatt tctacgatat
     4921 tgccgcatac gttacatttg tagacttcgt ttctgttagc catcataaac ctccttgttt
     4981 tattatgatt aatctacttt cttaaacatc tttcccttgg cgccgcagac cggacaggtt
     5041 tccggcgctt cgttttccac ggtataaccg cagaccggac aaacataata gctgtatgtt
     5101 tcctgtgaac cgtccagatg atccagcgct ttctgataga gggcggcgtg aattttttcc
     5161 acttcgttgg caaagttaaa agaccgctcc gcttctttat gactttcggc tttggccgct
     5221 tcaatcatgg ccggatacat gcttttgaat tcgtgggttt ctccggcaat ggcttcctga
     5281 agattttctt ttgtggaacg gatgcctttc atcgccctca aatgggcgtg ggcgtgtacg
     5341 gtctccgctt ccgctgccgc ccggaataat tttgccgcct gggcaaatcc ttcctgatcg
     5401 gccttggctg caaacgccag atatttccga ttggcctgtg attctcctgc gaaagcttcc
     5461 tttaatgctt cttctgtttt ggacattttc atatgctcct tcgttttatt tttttatgaa
     5521 tcaattaaaa atttgtcggt aaaggcatca cactccgaat tcagcgacgt ctgtatggct
     5581 gccgctgaat ccggggaatg atcaatttac gccaggtcaa aacggtccag attcatgacc
     5641 ttgttccagg ccgccacaaa gtcgtccagg aacttctcct gcgaatcttt acaggcatag
     5701 acctccgcca cggcccgaag ctgtgagttc gatccgaaaa tcagatcaac gcgggtgccg
     5761 gtccatttga gctcgccggt cttgcggtcg cgcccttcaa atacatccgc atctttcgac
     5821 accttccact ccgtgcccat gtcgagtaaa ttcacgaaga aatcgttggt gagcgttccc
     5881 ggacgcgagg tgaaaactcc atgcggagaa ccgccgaaat tggcgcccag caaacgcaga
     5941 ccgccgatca gcacggtcat ttccggcgcc gttagcgtga gaagctgagc cttgtccacc
     6001 agcatctctt cgggcaatac ggtgtatttg gccttctgat agttgcggaa tccgtcggct
     6061 tttggttcca gcacgccaaa cgaagctaca tcggtctgct tctgtgaagc gtccatgcga
     6121 cccggtgtga aaggaaccgt aattttgtaa ccggcattct tcgccgcctg ctcgacaccg
     6181 gcgcagccgg ccagaacgat cagatcggca agcgatacct tcttgtcgcc tttctggtct
     6241 ttgttgaacg ctttctggat gcgctccagc gttttgagca cgccggccag acgttccggt
     6301 tcattcacct cccaattctt ctgcggagcc aggcggatgc gggcgccgtt ggcgccgccg
     6361 cgcatgtcgg agccgcggaa cgtggaggct gacgcccagg ccgtcgaaac aagttccgat
     6421 atggacagac ctgaagctaa aatcttgctt ttcaaggaat tggcatccgc tttgttaatc
     6481 agcttatggt tgacggcggg aatggggtcc tgccatatca gcacttcttt ggggacctcc
     6541 ggaccgagat agcgcgaacg cggacccatg tcgcgatggg tcagcttgaa ccaggcgcgg
     6601 gcaaaagcgt cggcaaattc tttcg
//
LOCUS       DAOOLB010000101         6596 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_13879_length_6596_cov_45.715793, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000101 DAOOLB010000000
VERSION     DAOOLB010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13879_length_6596_cov_45.715793"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..888
                     /locus_tag="PLF77_09740"
     CDS             49..888
                     /locus_tag="PLF77_09740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPC09177.1"
                     /translation="MFKQLEEINTPPEPFQFYTAEDLWTDEHTSKHMLMFHLDEELDA
                     SSRNIAFIDRSVDWIVSRFNIGAGQRIADFGCGPGLYTTRLARRHAVVTGIDFSKRSI
                     QYAQETAIGEKLPIRYVNRNYLDFETDERFDLILMIMCDFCALSPAQRKTMLAKFHRL
                     LDPAGSVLLDVYSLNAFNEREEKALYEPNLLDGFWSPDKYYGFLNTIKYEKEKVVLDK
                     YTIIEAERTRTIYNWLQYFSPEDVEREFSACGFAIENYYADVAGTPFSAQSREFAIIA
                     RKL"
     gene            complement(920..2131)
                     /locus_tag="PLF77_09745"
     CDS             complement(920..2131)
                     /locus_tag="PLF77_09745"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HPC09178.1"
                     /translation="MTISAFEPATLAGIKLKNRIIRSATYEGMADEKGFPTEKLKKLY
                     INLAKGDVGAIITGYAATQADGKVSNAMAMVDHDDKINVFKEITDAVHEYGTPVIMQI
                     AHCGRQTRSRITGFPTVAPSPVRNMYFMEDKPKALSEEGINEIIDNFVAAIVRAKRAG
                     FDGVQLHLAHGYLLSDFLSSHANRRKDRWGGSTENKYRIVGEIFKRARGKVGDYPILV
                     KMNAYDGRRKGMRIEEAVVIARMLENSGCAAIEVSCGCVEDGLFTIRGEKLPVEAVVE
                     YNFNFKNYPDFAKKTIIWFAKTFLKTPKPLLKYNLDAAMQIKKAVNIPVIVVGGINNV
                     EDVEDIIGNDKADFVSMSRPFIIEPDIVSKFKNRTQTTSKCIMCNFCVITIEKEPLKC
                     RYGKLPKTKSA"
     gene            2338..4395
                     /gene="tkt"
                     /locus_tag="PLF77_09750"
     CDS             2338..4395
                     /gene="tkt"
                     /locus_tag="PLF77_09750"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPC09179.1"
                     /translation="MSDKNELEKQCIDAIRFLAADAIEKAKSGHPGMPLGAAAAAFTL
                     WTKHLKHNPKNPQWPDRDRFVLSAGHASMLLYALLHLTGYDLTLDDIKTFRQWGSRTP
                     GHPEYKHVPGVEVTTGPLGQGLANAVGMAVAEAHLAARFNGEDQKIVDHFTYVMAGDG
                     DMMEGVAGEACALAGHLRLGKLIVLYDDNKVTLAGSAALSSTENIEQRFKAYGWQVLK
                     VADGNDVAAIDKAIKKAKREAEKPSLICIASTIGYGAPCKQGSCDAHGSPLGADELQK
                     AKEALCWLADSPFSVPENVYTFFRKAVSRGKKDEKKWQESFNNFRQNEPALAAEFDRI
                     MKGDLSDGWESALSEFPNGSKDVATRKAGEIVMQSIAGKIPELAGGSADLNPSCFTWL
                     KGMGDFQSPKLSKEGLHGMVGGAWDYSGRNIHFGVREHAMGSIAVGMALHGGIIPYTA
                     TFLTFADYMRPPMRLAAMMGLRAVFVFTHDSIGVGEDGPTHQPVEQIMNLRQVPNMTV
                     IRPADANETLEAWKIAITNIKGPTTLIFSRQNLPVLDRSVYASASGTKQGGYVLWESA
                     SDPDVILIATGSEVSLTLTAARKLAEAGTKTRVVSMPSWELFDRQPRDYRDKVLPPQI
                     SARIAVEAGIKLGWEHYVGLNGKIIGMETFGASAPGPVLYEKFGFTVEKIIEAARDLL
                     HRS"
     gene            4537..6204
                     /locus_tag="PLF77_09755"
     CDS             4537..6204
                     /locus_tag="PLF77_09755"
                     /inference="COORDINATES: protein motif:HMM:NF012562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09180.1"
                     /translation="MTETRIDYSLGEYQAAVKSALEKMRHDNVIERIRSKDYTLWKPR
                     PDEIVNRLGWIDAPAETLAKINDIRSVVDSLQKDKISDIVLIGMGGSSLAAEVFGKIF
                     GKKKSYPRLNVVDTTDPVFISEVTQHLNLEKTLFLVSSKSGSTLEITSLFKYFYNLCL
                     KKFGGEAGRRFIFITDEGSPLIKTAESISARHIFLNNPDIGGRYSALSLPGIVPAALI
                     GIAVETLLQNVSSNLEALMSAGASLGAALGMLANQGRNKMTFVLPPHWKHFGDWLEQL
                     IAESTGKEGRGILPVLNETLLDASGYGKDRIFVIFQNKNEEEPSQMETLKKDGHPVIT
                     VKINDDYELGQQMYIWEMATAVAAHFMGVNPFDQPDVEATKKHTRAVIADLDKRGELA
                     DEKPAITFEQAEIFGNINGATPADVIRNFMKQAKAGDYICLQVFLIPSQETNEAVNSL
                     RKVLACQYTLPVACGYGPRYLHSTGQLHKGDAGNGLFIQLTQDFSLDVDVPDSIGEEK
                     SFLTFSALKAAQAKGDRQALTEAGRRIIRIHFKKDPVDGLKTIVNGL"
     gene            complement(6340..>6596)
                     /locus_tag="PLF77_09760"
     CDS             complement(6340..>6596)
                     /locus_tag="PLF77_09760"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPC09181.1"
                     /translation="GSKSVKSLDVDCALAASYIMFSAVERGLGTCWVNLGSNIRDPQS
                     RALLAIPDGCRIVAPIILGYPKTIPPVSERHAPEILRVIS"
BASE COUNT         1632 a         1641 c         1676 g         1647 t
ORIGIN      
        1 gttcattcgc agcgctgttc tgtgtggcca agagaataaa agacaacgat gttcaaacaa
       61 cttgaagaaa taaacacccc tccggagccg tttcaatttt acacggctga ggatctctgg
      121 acggatgaac atacctcaaa gcacatgctt atgttccacc tggatgaaga actggatgcc
      181 tcgtcccgaa acatcgcttt catcgatcgg tcggtggatt ggattgtctc ccgttttaac
      241 attggcgctg ggcagaggat tgccgatttc ggctgtggtc ccggattata tactacaagg
      301 ctggcccgaa ggcatgcggt ggtcaccggc atagatttct cgaagcggtc aatccaatac
      361 gcgcaggaaa cggcaatcgg ggaaaaactg cccattcgtt atgtcaaccg gaactatctg
      421 gattttgaaa cggacgagcg ctttgacctc attttgatga ttatgtgcga tttttgcgcc
      481 ctcagccccg ctcaaaggaa aacgatgctt gccaaatttc atcggcttct cgatcccgcc
      541 ggttcggttc tccttgatgt gtattcctta aacgccttca acgaacggga agaaaaggct
      601 ctgtatgaac ccaatcttct cgatggcttc tggtctcccg ataaatatta cggttttctg
      661 aatacaatca agtatgagaa ggaaaaagtg gtcctcgata aatacacgat cattgaggct
      721 gagcgcacgc gcacaatcta taattggttg cagtatttca gtcctgaaga tgtggagagg
      781 gagttttccg cctgcggttt tgccatcgaa aattattatg ccgatgtggc gggcacgcct
      841 ttttctgcgc agagcaggga atttgcgatt attgcaagga aactttaacg cgcacaacat
      901 gctgatccgg aagatggttt taagcgcttt ttgttttcgg cagtttgccg tagcggcact
      961 tcagcggttc tttctcgatg gtgatcacgc aaaaattgca catgatgcat tttgatgtag
     1021 tctgcgtccg gtttttgaat ttgctcacaa tgtccggctc gatgataaac ggccggctca
     1081 tggagacaaa atccgcctta tcattcccga taatgtcctc aacatcttcg acattgttga
     1141 tgccgccgac aacgatcacg ggaatgttca cggctttttt aatttgcatt gccgcatcga
     1201 gattgtattt caacagcggt ttgggcgttt tcagaaatgt tttggcaaac cagatgatcg
     1261 ttttcttcgc aaaatccgga taatttttga aattgaaatt gtattccacg acggcttcca
     1321 cgggaagctt ttctccgcgg atggtgaaaa ggccgtcttc cacgcaaccg caggacactt
     1381 cgatggccgc gcagccggag ttttccagca tccgggcgat gacaacggct tcttcaattc
     1441 tcatgccttt tcgccgcccg tcataggcgt tcatttttac aaggatcggg tagtcgccga
     1501 cttttcccct ggctcttttg aaaatctcgc cgacgatgcg gtatttgttt tctgttgagc
     1561 cgccccagcg gtctttacgg cgatttgcat gcgaggataa aaaatcggac agcaggtagc
     1621 cgtgcgccag atgaagctgg accccgtcaa atccggctcg ttttgcccgg acgatggcgg
     1681 caacaaaatt gtcgatgatt tcgttgatgc cctcttcgga caaggctttc ggtttatcct
     1741 ccatgaaata catattgcgc accggggagg gggcaacggt cggaaagccg gttattctgg
     1801 agcgggtttg cctgccgcag tgggcgattt gcatgataac cggcgtgccg tattcatgaa
     1861 cggcatctgt aatttcttta aaaacattga ttttgtcgtc atgatcgacc atggccatgg
     1921 cgttgctgac cttgccgtcc gcctgagtag cggcgtatcc ggttatgatt gcgccgacat
     1981 ccccttttgc cagattgatg taaagttttt ttagtttttc ggtggggaat cctttttcat
     2041 cggccatgcc ctcataggtc gccgaacgga ttatccggtt tttcaatttg attcctgcca
     2101 atgtagccgg ttcaaatgcg gaaattgtca tatatgataa cttcccccgt ttattttgat
     2161 gagttgaatc attggtttag acataagcta acttattgtt tataatgcat aatctctaaa
     2221 tgtttatttt gcaatcattt tccgtgcacc gcggtaaaat ttattccaag aaaagtttaa
     2281 aaaaaacttc aaatgtgtta gtacatcacc atccttacga taattaaagg agtcttcatg
     2341 tctgataaaa acgagttaga gaaacaatgt attgatgcca tccgctttct cgctgccgat
     2401 gcgatcgaaa aggccaaatc cggccatccc ggtatgccgc tgggcgcagc agccgcagcg
     2461 tttacgctct ggacaaaaca cctgaaacat aaccccaaaa atccgcaatg gcccgaccgc
     2521 gaccgttttg tgctttccgc cggtcatgcc tccatgcttt tatacgctct tctgcatctg
     2581 accggttacg atttgacgct cgacgatatt aaaactttcc ggcagtgggg aagccgcacg
     2641 ccggggcatc cggaatacaa acatgtgccc ggtgtcgaag tcacaaccgg cccgctgggt
     2701 cagggactgg ccaatgcggt gggtatggcg gttgccgaag cgcatctggc tgcgcgtttt
     2761 aacggggaag atcagaaaat agtcgatcat ttcacttatg tcatggccgg tgacggcgac
     2821 atgatggaag gcgtggctgg tgaggcctgc gctctggccg gccatctgcg cttgggaaaa
     2881 ctgattgttc tgtatgacga taataaagtg acgctggcgg gatccgcggc cctttcttcc
     2941 acggaaaaca tcgaacagcg ttttaaggcc tacggctggc aggtgctcaa agtcgccgac
     3001 ggcaacgacg ttgccgccat cgacaaggcc attaaaaaag ccaagcgcga agctgaaaag
     3061 ccttcattga tttgcattgc atccacgatc ggttacggcg caccctgcaa acagggaagc
     3121 tgcgatgcgc acggctcacc gctgggcgcc gacgagctgc agaaggccaa agaggctctc
     3181 tgctggctgg ccgattctcc tttttctgtt cccgaaaacg tttatacgtt tttccgcaaa
     3241 gccgtgtcgc gcggcaaaaa agacgaaaaa aaatggcagg aatcgtttaa caacttcaga
     3301 caaaatgaac ctgctttggc agcagaattt gaccgcatca tgaaaggcga cctgtccgat
     3361 ggctgggaat cggctttgag cgaattcccc aacggatcaa aagacgtggc cacccgcaaa
     3421 gcgggcgaaa tcgtgatgca gtccatcgca ggcaaaattc ccgaactggc gggcggctcc
     3481 gccgatctga atccgtcctg ttttacctgg ctcaaaggca tgggcgattt tcagagtcct
     3541 aaattatcca aagaagggct gcacggcatg gtcggcggcg cctgggatta ctccggacgt
     3601 aatattcatt tcggcgtacg cgaacatgcg atgggctcga ttgccgtcgg catggcgttg
     3661 cacggcggca tcattcccta cacggcaacc tttctgacct ttgccgatta catgcgtccg
     3721 cccatgcgcc tggcggcgat gatgggcctg cgcgctgtat tcgtctttac gcacgacagc
     3781 atcggcgtcg gagaagacgg cccgacacac cagcctgtcg aacaaattat gaatctgcgt
     3841 caggtaccca acatgacggt aatccgtccg gcggacgcca atgaaacact ggaggcctgg
     3901 aaaatcgcca ttaccaatat aaaagggccg acgacactga tcttcagccg ccagaacctt
     3961 cccgtgctgg acagaagcgt ttacgcatcc gcgtccggca ccaaacaggg cggttatgtt
     4021 ctgtgggaat ccgcgtccga tcccgacgtg attctgattg ccaccggttc ggaagtttca
     4081 ctaaccctga ccgcagcccg caagcttgcg gaagccggca cgaaaaccag agttgtttcc
     4141 atgcccagtt gggaactatt cgaccgtcag ccgcgcgact atcgcgataa ggttcttccg
     4201 ccgcagattt ccgcccgcat tgccgtggaa gcaggcatca agctggggtg ggaacactat
     4261 gtcggtttga acggaaaaat catcggcatg gagacattcg gcgcttccgc acccggaccg
     4321 gttctttacg aaaaattcgg ctttaccgtt gaaaaaatta ttgaagcggc aagagacttg
     4381 cttcatcgct cctgacggga tagtttccaa tcccgataaa tcgggattgg atagttccgc
     4441 taacgcttca aactgcgcta cgcttttttt agcgagcgtc tctacaacta acgcttcgtg
     4501 tagttagcga gtctcattac attggagttt ccctaaatga ctgaaacacg cattgattat
     4561 tctctgggag agtatcaagc cgccgttaaa agcgccttgg agaaaatgcg ccatgacaac
     4621 gtcattgaac gcatccggtc aaaagattat accctctgga aaccccggcc cgatgaaatc
     4681 gtcaatcgcc tgggatggat tgacgctccg gctgaaacgc tggcaaaaat aaatgacatc
     4741 cgcagcgttg tcgattctct gcaaaaagat aaaatcagcg atatcgtttt aatcggcatg
     4801 ggcgggtcat cgcttgcggc agaagtcttt ggcaaaatct tcggcaaaaa aaaaagctat
     4861 ccacgattga atgtcgtcga tacgacagat cccgttttta tttctgaagt cacacaacat
     4921 ttaaatcttg aaaaaacctt atttcttgtt tcatcaaaat caggttcaac cctggagata
     4981 acatcgctct tcaaatattt ttataacctg tgtctgaaaa aattcggcgg cgaagcggga
     5041 cgccggttca tttttatcac cgatgaggga agtccgctga tcaaaacagc cgaaagtatc
     5101 tccgcccgtc atattttcct caacaatccc gatatcggag gacgttactc ggcgctgtcg
     5161 ctgccgggaa tcgttccggc ggcattgatc ggcattgctg tggaaacgct gctgcaaaac
     5221 gtttcatcca atttggaagc gctcatgagc gccggcgctt ctctgggcgc cgctttagga
     5281 atgctggcca accagggtcg caacaaaatg acgtttgtcc tgccgccgca ctggaaacat
     5341 tttggcgact ggctggaaca actgattgcg gaaagcacag gcaaggaagg caggggaatt
     5401 ctccctgtgc tgaacgaaac gcttttggat gcttcaggat atgggaaaga ccggattttt
     5461 gttattttcc aaaataaaaa tgaagaagag ccctctcaaa tggaaactct gaaaaaggac
     5521 ggtcatcccg tcattaccgt taaaattaac gacgattatg agctgggtca acaaatgtac
     5581 atctgggaaa tggcgaccgc ggttgcggct cattttatgg gtgttaatcc ttttgatcag
     5641 ccggatgtgg aagcgactaa aaaacatacc cgcgccgtga ttgccgattt agacaagcgt
     5701 ggagaattgg cggatgaaaa accggcaata acctttgaac aggctgaaat cttcggcaat
     5761 attaacggtg cgactccggc agacgttatc cggaatttta tgaaacaggc caaggcgggt
     5821 gattatatct gtctgcaagt tttcttgatt ccgtctcagg aaacgaatga ggcagtcaat
     5881 agtttacgta aagttcttgc atgccaatat actttgccgg tggcctgcgg atacggtccg
     5941 cgctatctgc attccaccgg gcagttgcac aagggcgatg ccggaaacgg cttgttcatt
     6001 cagttgacgc aggatttttc tttagacgtt gatgttcccg acagcatcgg cgaagaaaaa
     6061 tcctttctca ctttcagcgc gctcaaagcc gcgcaggcga agggcgaccg gcaggcgctg
     6121 acggaagcag ggcgaagaat catccgtata catttcaaaa aagatccagt cgatgggtta
     6181 aaaacaatag tcaatggtct ctaagcaaat ctcaatattg gcgagttatt cagttgtcag
     6241 gctggagcgg gcaatccagt tgtataaaga cctggccgcc cgcttacgcg ggaacggcgc
     6301 ctggattacc gcctgtgcgg gaatgataca ttatttcatt taactgatca cccttagaat
     6361 ttccggcgcg tggcgttcgg ataccggcgg aattgtcttt ggataaccga gaataattgg
     6421 cgcgacaatc cggcagccgt cgggaatggc cagcagggcc ctggactgag gatcccggat
     6481 gttggatccc agattgaccc agcaggttcc cagtccgcgc tcgacggcag aaaacataat
     6541 gtagcttgcc gccagcgcgc aatccacgtc aagcgacttg acgctctttg aaccga
//
LOCUS       DAOOLB010000102         6549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_14025_length_6549_cov_35.996766, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000102 DAOOLB010000000
VERSION     DAOOLB010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14025_length_6549_cov_35.996766"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="PLF77_09765"
     CDS             complement(<1..399)
                     /locus_tag="PLF77_09765"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPC09182.1"
                     /translation="MVLINYRSDRHMMITGKKKKSIGRSPGIFLAVFFMLWMVLPVHE
                     SWAATGYPKSRGAVNDFAGVIDAENAAKMEALAQEVLQKTGTAVVVATVETIGENEDY
                     NMYANGLYQAWGIGKKGEDKGVLIFLTVKER"
     gene            517..2067
                     /locus_tag="PLF77_09770"
     CDS             517..2067
                     /locus_tag="PLF77_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HPC09183.1"
                     /translation="MTPKIILIHPPVAKCSEPPAGIARLASCLNANAIPYETIDANLE
                     GLLYLLKSASGNNTLSDRWTKRACKNLDRHLAAMKNIGTYQNPGRYGQAVKDINRLLN
                     VAAKPFHVALSLTDYRDRRFSPVKNADLIASAHNPQANPFYPYFKNRLTNALRSNPDF
                     IGLSLNYLSQALCTFAMIGFIKNIHPRQKIILGGSLVTSWMKLGAHLHTFGGLVDEIV
                     EGAGEQKLLEILGTQNKLIHNAPVYDDFPLDQYLSPGLIIPYSGARGCYWHQCAFCPE
                     KAEGNAYRPLEASQVTKELQELARQTNPALIHLLDSSISPSLLQALSQNPPGTPWYGF
                     ARVTAHLADEDFCLSLKKSSCAMLKLGIESGDQNVLDQLNKGIDLLTASAVLKNLKKA
                     GIATYCYFLFGTPPENENSAIKTMNFMADHHDCIDFLNLAIFNLPVRSVESDSLATYD
                     FYAGNLSLYRNFKHPLDWHRPAVRHFLENVLKKHPAISLILKRNPVFFTSNHAPFFVL
                     NGLSHEHF"
     gene            2228..3502
                     /locus_tag="PLF77_09775"
     CDS             2228..3502
                     /locus_tag="PLF77_09775"
                     /inference="COORDINATES: protein motif:HMM:NF018271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09184.1"
                     /translation="MIFDDITISSFILFFAVYAFMGWVIEVIYRSVSQRQLINAGFLF
                     GPFIPIYGFGAVFIILLEFLIHPWPLPVKIAFYGIILTGIEYLTSHIMEKAFKLKLWD
                     YSNNKFHIHGRVCLLFSIFWTAMALGFITFLHPVVSRFIASWDAALMNMLAAVFLAYS
                     VTDFVFSVMSLTSFRRKIAYLYSEYFNLSNAEIEKIFNSFKRLRTAFPNLSKYIENNI
                     NAEIKNKISSFVKSIPAKIIPGDYGRKPAESEFYAIISDIYEHEEFVKLKEHYHHNSS
                     IYEHVMDVSYFSYKACKLLKLDHHSAARGALLHDFFLYDWRNHDVPDLPEDKFHGIEH
                     PKIALANAQKHFQLNDIETDIIIKHMWPLTLVPPKYKESFIVSFADKYLASKEFVTKF
                     KEGQIKNRGAKKSGTRRSHKRHKKRQKTPQKK"
     gene            complement(3516..5165)
                     /locus_tag="PLF77_09780"
     CDS             complement(3516..5165)
                     /locus_tag="PLF77_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09185.1"
                     /translation="MINPIISNIQNNKNIVNIFLIFISLFLLTIIPIDNIPVHDQNAD
                     SYNGELTAYSLNFVNNWLKDGIINDKFMTYCDYASVEFENNNNRCVYLSYPPGAFLPL
                     YFIAKGIGAKEVSTGFIKYFIQLEYYLSILLLALLFYVCLKILEVRSRLIMIVLPVIF
                     SSLWAFLPYNLYYMKNIYFTDQAVILLSIVFFLIEFALYDTSLGAWKKILQGLSCAVL
                     FAGILTDYYFYCIAFAAISFRILTAFQDHPRKSILYKLFSDTWPLIVTAIVATALFII
                     QLIWSPNGLKLLVLTFLIRADSGADFGGVNGLINHFNIGFSVFSIPVLMMTALFCFIF
                     PFIRNRYGQDKQMLVRWLSIIILPAVLHTVILREHSIIHEFSMLKYNLVFVFIIFSFV
                     CWIYLSYRTREAGGVRKVLCLAVLFILGVLLFSFRNLNAYGQFFYEKRMFTDNHSIAH
                     FIKNHTNYFDVVYSPDYEIYWNQPQDLAISQKRVYKISSLEEIPYDRLPEHAVINILI
                     SCKTLKNPVWSKLGTGGSFSGKSNDLYLFKFRGKSFRSVID"
     gene            complement(5158..>6549)
                     /locus_tag="PLF77_09785"
     CDS             complement(5158..>6549)
                     /locus_tag="PLF77_09785"
                     /inference="COORDINATES: protein motif:HMM:NF005546.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HPC09186.1"
                     /translation="RFFSKSDTVMKWWQEILPIQGNESENGARKTAFSTGGPDPEKTD
                     RVMLVRSRLSRIFYLGKFFDYPIKLNWKTVSNLGMIRIAKIGWSYLRVKIFSVKQIKS
                     LEDFFVNRFGQELYETFFKDYTQKIWGVPCNEIAADWGAQRIKELSVSRTLLHAFKAL
                     YSKDKSIEQKNTDTSLIGRFMYPKLGPGQMWETVARLVEEKGGVIIKNAEVTDVRMAD
                     HRIHELTYYNHSTNTKSTVAGDYFISTMPIQDLMSALGSAVPEDVKNVSDGLPYRDSV
                     TVGLLLNELKIKNETTIPTLNHLVPDNWIYIQERDVKVARMQIFNNWSPYLVADIRKV
                     WIGLEYCCNQGDELWQKPDSDFIQFAIDELHKIAMIDKNEVIDSVIIRTPKTYPAYFG
                     TYSRFGVIRHFTDSIENLFLIGRNGMHKYYNQDHAMLTAMTAVNNIISGETAKDNIWN
                     INVGEEYIETEND"
BASE COUNT         1886 a         1513 c         1312 g         1838 t
ORIGIN      
        1 ccgttctttg acggtcagaa aaattaatac gcctttatct tcgccttttt tgccgatccc
       61 ccaggcctga taaagcccgt tggcgtacat attgtaatct tcattctcgc cgatggtctc
      121 aaccgttgcc acgacaactg cggtgccggt tttttgcaaa acttcctgag ccagcgcctc
      181 catttttgcg gcgttttccg catcaatgac accggcaaaa tcattaaccg ctccgcgcga
      241 cttcggatac ccggtcgccg cccatgattc atggacgggc agaaccatcc aaagcatgaa
      301 gaagacggca aggaaaatcc ccggagaacg gccgatactt ttctttttct tgccagtaat
      361 catcatgtgt ctgtcagagc ggtagtttat taataccatg aactattgct taaagtttta
      421 tggctgtcaa gttcaaagca gaatgccaaa atccttcttg cgtatgcgca gattacgtaa
      481 tagaaaaaaa cagtttttca aaggtaacgt tttattatga cgcctaaaat cattctgatt
      541 catccgccgg ttgccaaatg ttcggagccg cccgcgggga tcgccagact ggcttcctgt
      601 ttgaatgcca acgccatccc atatgaaacg attgatgcca atctggaagg tcttttgtat
      661 ctgttaaaga gcgcttcggg aaacaacacc ttatccgaca gatggacaaa gcgcgcgtgc
      721 aaaaatctgg atcgtcatct tgctgcaatg aaaaacatcg gcacgtacca aaatccgggt
      781 cgctatgggc aggccgtcaa ggacatcaac cgtcttttaa atgttgcggc aaaaccgttt
      841 catgttgcct tgtcactgac cgattaccgg gacagaagat tctctccggt taagaacgcc
      901 gatctgatag cgtccgccca caatccgcaa gccaacccct tttaccctta ttttaaaaat
      961 cgtctgacaa acgccctgcg gtcaaatcct gatttcatag gcttgtcttt aaactacctg
     1021 agtcaggcgc tctgcacctt tgccatgatc ggttttataa agaacattca tccgcgacaa
     1081 aaaattattc tgggcggatc gcttgtcaca tcgtggatga agctgggcgc acatctccac
     1141 accttcggcg gtctggttga cgaaattgtg gaaggcgcag gagagcaaaa attgctcgag
     1201 atactgggca cacaaaacaa gctgattcat aacgcccctg tatacgatga ttttccgctt
     1261 gatcaatatt tatcgcccgg tctgatcatt ccctacagcg gtgcgcgcgg ctgttactgg
     1321 catcaatgcg ctttctgccc ggaaaaagcc gaaggaaatg cttatcgccc tttggaagct
     1381 tctcaggtca ccaaagaact acaggaatta gcccgtcaaa cgaatccggc actgattcat
     1441 cttctggata gttctatttc accgtcgctt ttacaggctc tgtcacagaa tccacccggc
     1501 acaccatggt acggttttgc ccgggtcact gcacatttgg ccgatgaaga tttctgttta
     1561 tctttgaaaa aaagcagctg tgccatgctg aagctgggta ttgaatccgg cgatcaaaat
     1621 gttctggatc aactcaataa aggaattgat ttactgacag cctctgccgt tttaaaaaat
     1681 ttgaaaaaag cgggtattgc cacgtattgt tacttccttt tcggtacgcc gccggaaaat
     1741 gaaaatagcg caatcaaaac catgaacttt atggccgatc atcacgattg tatcgatttt
     1801 ttgaatctgg ccatttttaa tttgccggtc cggagtgtcg aatcggacag tctggcgacg
     1861 tatgatttct acgccggcaa cttgtctctt tatcgaaact tcaaacaccc cctggactgg
     1921 cacagacccg cggttcgcca ttttctggaa aatgttttaa aaaaacaccc ggccatctct
     1981 ttgatcctta aacgaaatcc ggtttttttt acgtccaacc acgccccttt ttttgttctc
     2041 aacggattgt cacatgagca tttttagcaa aatatcgccc gcagagactt gttgacaacg
     2101 cttggccaat attataggta tggcaaccaa tccgctccgg cttgacatga cataaaccga
     2161 tgaacttaag cctccggatt caacgaacac attgctgcaa gacccaaaca tgagatagat
     2221 atatccaatg atttttgacg atataaccat atccagtttc atcctttttt ttgctgtcta
     2281 tgcttttatg ggatgggtca tcgaggtcat ctacagatct gtttcacagc ggcagctgat
     2341 caatgcaggc tttcttttcg gtccctttat tcccatttac ggtttcggag cggtttttat
     2401 tatccttctg gaatttctga ttcatccgtg gccgctgccg gtcaaaattg ccttttatgg
     2461 aattatttta acaggcatag agtatttaac cagccatatc atggaaaaag ctttcaaact
     2521 gaagctctgg gattattcga acaacaaatt tcatattcat ggccgtgtct gtctgctttt
     2581 ctccatattc tggacggcaa tggctttggg attcatcact tttcttcatc cggtcgtttc
     2641 acgctttatt gcatcgtggg atgctgcttt gatgaacatg ctcgcggccg tttttctggc
     2701 ttacagcgtc acggattttg tgttttctgt tatgtccctt acatcgttca gaagaaaaat
     2761 cgcttatttg tactccgaat atttcaatct gagcaatgct gaaatcgaaa agatatttaa
     2821 ttcctttaaa cgtctgcgga ccgctttccc taacttgagc aagtatattg aaaataacat
     2881 caatgctgag ataaaaaaca agatcagctc ttttgtaaaa tccattccgg ccaaaatcat
     2941 tcccggagat tacggacgga aaccggcgga aagcgaattc tatgcaatta tcagcgacat
     3001 ttacgaacat gaagagttcg tgaaactcaa agaacattac catcacaact cttccatcta
     3061 cgaacacgta atggacgtct cttatttttc ctataaagct tgcaagctcc tgaagctgga
     3121 tcatcactct gccgcccgcg gcgcactcct gcatgacttt tttctatacg actggagaaa
     3181 tcacgatgtt cccgatctcc cggaagacaa attccacggt atcgaacacc ccaaaatcgc
     3241 gcttgccaac gctcagaaac atttccaact aaacgatatc gaaacggaca ttatcatcaa
     3301 gcatatgtgg ccgctgacgc tggttccgcc aaaatacaag gaatccttca tcgtatcgtt
     3361 cgcagataaa tatcttgcgt ccaaagaatt tgtcacgaaa ttcaaggaag gacagattaa
     3421 aaaccgcggc gcaaaaaagt ccggcacgcg gcgttcccac aaacggcata aaaagagaca
     3481 gaaaaccccg cagaaaaaat aatctctcta tcgttttaat caatcactga acggaaggat
     3541 ttcccgcgga atttaaacaa atacagatca tttgattttc cggaaaagga cccgcctgtg
     3601 cccagtttgc tccagactgg attcttgaga gttttgcaag agatgaggat attgatcacc
     3661 gcgtgttccg gcagacgatc ataaggaatt tcttcaaggc ttgaaatttt ataaactctc
     3721 ttttgagata tggccaggtc ctggggctga ttccagtaaa tttcataatc aggtgaatac
     3781 acaacgtcaa aataattcgt atggtttttt ataaaatgag caatggaatg attgtccgta
     3841 aacattcttt tttcgtagaa gaattgccca tacgcattta aatttctaaa actgaaaaga
     3901 agcacaccaa ggatgaagag cactgcaagg cacaatacct tccggacgcc gccggcttct
     3961 cgggtcctat agctcagata tatccagcag acaaacgaaa atataataaa tacaaaaaca
     4021 aggttatatt ttaacatgga aaattcatga ataatggaat gctcacgaag gatgacggtg
     4081 tgcaagacgg ccggtaaaat aatgatactc aaccagcgga caagcatttg cttatcttga
     4141 ccatatctat tccgtataaa tggaaatata aaacaaaaaa gcgccgtcat cattaaaacg
     4201 ggtatggaaa aaacagaaaa gccgatatta aaatgattga tcagtccgtt tacaccaccg
     4261 aaatccgctc cgctgtcggc tcttatcaaa aaagttaata ccagcaattt taagccgttc
     4321 ggactccata tcagctgaat gataaataaa gccgtggcaa ctattgctgt cacgatcagc
     4381 ggccacgtat cggaaaacaa tttatacagg attgatttcc ggggatgatc ctgaaacgcc
     4441 gttaatatcc gaaaggaaat tgcggcgaat gcgatgcaat agaaatagta atcggttaaa
     4501 atgcccgcaa acaacactgc gcaggaaaga ccctgaagga tttttttcca tgctcccaat
     4561 gacgtatcat ataatgcaaa ctctatgaga aaaaaaacga ttgacaataa gatgaccgcc
     4621 tggtccgtaa aatagatatt tttcatgtag tataagttat agggaagaaa cgcccataat
     4681 gaagaaaata ttaccggcaa aacaatcatt attaatcggg atctgacttc aagaattttc
     4741 agacagacat agaaaagcag tgctaataac aggatggaaa gataatattc aagctggatg
     4801 aagtatttaa taaaacctgt cgatacttct ttcgccccga ttcctttggc gataaaatat
     4861 aagggcagaa atgctcccgg gggataagaa agatagacgc atcggttatt attattttca
     4921 aattccaccg aagcgtaatc gcaatatgtc ataaatttat cattaatgat tccatctttc
     4981 agccagttgt tcacaaagtt caatgaataa gccgtaagct cgccattata actatcggca
     5041 ttctgatcat gaacgggaat gttgtcgatc ggtatgattg tcagaaggaa caggctaata
     5101 aatattaaaa aaatattaac aatatttttg ttgttttgaa tattggaaat tatcggatta
     5161 atcattttcc gtttcaatat attcctcgcc tacattaatg ttccagatat tgtccttcgc
     5221 tgtttctccc gaaatgatgt tgttgacggc cgtcatggcc gtgagcatgg catgatcctg
     5281 attgtaatat ttatgcatgc cgtttctgcc gattaaaaat aaattctcta tgctgtcggt
     5341 gaaatgcctg attacgccaa accgcgaata agtgccgaag taggcaggat acgttttggg
     5401 cgttcgaatt atcacgctgt caataacttc gtttttgtca atcatggcaa ttttatgcaa
     5461 ttcgtcgatt gcaaactgaa taaaatccga atccggcttc tgccacaatt catcaccctg
     5521 gttgcaacag tactcgagtc ctatccagac ctttctgata tcggccacga ggtagggcga
     5581 ccaattattg aatatttgca tcctcgccac cttcacatcg cgttcctgga tatagatcca
     5641 gttatcgggg accagatgat ttaacgtggg aatcgttgtt tcatttttaa tcttcaattc
     5701 atttaaaagc aaaccaaccg tcacggaatc ccggtaaggc aaaccatcgc tgacgttttt
     5761 tacgtcctca gggacagcgc tgcccagcgc cgacatcaaa tcctgaatgg gcatcgtcga
     5821 aataaaataa tcgcccgcca ctgtggattt tgtatttgtg gaatgattgt aatacgtcaa
     5881 ttcatgaatc ctgtgatctg ccatcctgac gtcggtcacc tcggcgtttt taattatgac
     5941 accgcctttt tcctccacaa gacgggcaac cgtttcccac atttgtccgg gacccagttt
     6001 cggatacata aagcgcccga tcagactggt atccgtattt ttttgttcga tggatttatc
     6061 tttgctgtaa agtgctttga atgcatgaag cagcgtcctg gaaacagaca attctttaat
     6121 tctctgcgcg ccccagtcgg cggcaatttc attgcatgga acgccccaga ttttttgagt
     6181 ataatcttta aagaacgtct cataaagttc ctgcccaaat ctattaacga aaaaatcttc
     6241 cagagatttg atctgtttga cggaaaatat cttaacccgc aaatatgacc agccaatttt
     6301 tgcgatccga atcatgccga gattggatac ggttttccaa ttgagcttga tcggataatc
     6361 aaaaaacttt cccaggtaaa aaatgcggga caatctgctt ctaacaagca taacccggtc
     6421 agttttttca ggatcgggcc ctccggttga aaaagccgtt tttctcgctc cattttccga
     6481 ctcattgcct tgaatcggca gaatttcctg ccaccatttc atgaccgtgt ccgattttga
     6541 aaagaaccg
//
LOCUS       DAOOLB010000103         6525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_14095_length_6525_cov_41.712519, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000103 DAOOLB010000000
VERSION     DAOOLB010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6525
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14095_length_6525_cov_41.712519"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="PLF77_09790"
     CDS             <1..1380
                     /locus_tag="PLF77_09790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPC09187.1"
                     /translation="WMSPNEKAKKKAAELLKKGAIFAFGLSERTHGADIYSTETTLTP
                     ADNGTWVANGEKYYIGNGNEAEMVSTLGKVKDGTDDYVFFVTNYKHKAYELKKNVISH
                     QEYVSNFALHDYPITEDEILMRGPHAWDSALNTVNIGKFNIGPASIGVVEHCFYEAIT
                     HASNRILYGMKVTDMPHVRKNFMDAWLRLVAMKLYQRRSTDYFRNANDKDRRYLLYNP
                     TSKMKVTLQSEDVLNLLWEVIAAKGFEKDTYFHMAAADIRGPSKLEGTVHVNVQLIRK
                     FMKNYFFNPVSYAPVAPDFSAKDDLFLFNQGPTKGLGSVQFHDYKPVFEAGRKLPNVE
                     IFIRQINIFKEMLEKAGPDKAQDLDPSWSLPVGEMFSIVVYGQLILEQAAFDKVDDDI
                     LNQIFDFMVRDFARFALQIYGMHNTKEDQRAYCKDIMLIKGQGDDAQYDRVWQKYVAV
                     LNGEYAMSE"
     gene            complement(1453..2250)
                     /locus_tag="PLF77_09795"
     CDS             complement(1453..2250)
                     /locus_tag="PLF77_09795"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HPC09188.1"
                     /translation="MFQWFINRRRSKLTAAPFPDAWEDILERNMGHYRLLHDAERAHL
                     RSLIQVFIAEKHWEGAGGLVLNDEIRVTIAAHACLLLLRLSHNYYRNVETVIVYPSTV
                     VAPRRKPGFFEVSLEPLEENYPIVGEAFQQGPVIIVWDAALQGGRQSESGHNVVYHEF
                     AHKLDMLDGAADGTPPLKDRAKYRDWIKTFSHEYLRLRRDTEKGRQTFLDAYGATNEA
                     EFFAVATEHFFEQPLIMEKNAPELYRRLREYYGQDPASRFRQNNCAV"
     gene            complement(2430..2993)
                     /locus_tag="PLF77_09800"
     CDS             complement(2430..2993)
                     /locus_tag="PLF77_09800"
                     /inference="COORDINATES: protein motif:HMM:NF027363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HPC09189.1"
                     /translation="MFKKLLLLCSVVLILTSVGYAKEVGGINMPDTLAAGTDTLILNG
                     AGIRTKFFIKAYVTGLYLKKKNSDANAILNADEPMAVRIHIISKLITSDKMKDATMEG
                     FQNSTNGNIAPYKDKIDTFISVFADNIKINDVYDLVYTPAEGTQVYKNKQLKTTTKGL
                     DFKKVLFGIWLCNKPADKALKSQMLGK"
     gene            complement(3234..4457)
                     /locus_tag="PLF77_09805"
     CDS             complement(3234..4457)
                     /locus_tag="PLF77_09805"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HPC09190.1"
                     /translation="MPRIFDPNDKYYIDNPGRVKAIQAEMHKRGIDVYLGSRIRTMSF
                     VLDAFCPWRSYLIIPSEGQPAFFTFVIDALRVGDETWLDSDHVRGYGPMGGMDQVSAI
                     TDYIKDELGIKKGRLGYEDGVSTYTAEGNLSHWEYTRLRDALPDFEWVNAHDIIDGLS
                     LIKDKGTIERFRTASLIVDEGHKAARAALENGGYRGMTETVIAGIAALAMRKAGSVSE
                     WNFAGLNEISSGYRTGLGACTPPTTKEIKAGEPLMLDFHAMFKLALGDHSHNYLIDPA
                     TKRQRWHADNFVSIIRKVIDNYRAGTTPGKLAAMMMDFAESRGCADFILPGCEHGIGL
                     FGDEWRIGSIVNPDLPYWTDQDHVYQENEMVVCAMQYAAPEEDIGFRYENDIVITKDG
                     CDVMSKYPLGIEEIS"
     gene            complement(4469..4873)
                     /locus_tag="PLF77_09810"
     CDS             complement(4469..4873)
                     /locus_tag="PLF77_09810"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HPC09191.1"
                     /translation="MSELLAYLSPAWCDEAFKRLQTELTPDKMNKVSTSMSNIYKNCP
                     GGKEMFLFIECKEGNITRVETGVGDPPKAEFLIIGDYETFSRISRAELGSQKALMTGK
                     IKLKGNLAKALKLAAVSDRLNKVLAQIPTQYG"
     gene            complement(5163..5633)
                     /locus_tag="PLF77_09815"
     CDS             complement(5163..5633)
                     /locus_tag="PLF77_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(5502..5504),aa:Sec)
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HPC09192.1"
                     /translation="MARVELNVQAPDFTLKDFNGTIVSLSDFQNKKNVMLVFNRGFIU
                     PFCRVHMAQLRQDYEKFVKLDTEILVAGPEDAEAFKKYWVKEKLPFVGLPDPEHHVLK
                     LYGQEVKIFKLGRMPAQIIIDKSGKVRFVHYGHSMMDIPENKELLELLATQIEK"
     gene            complement(5721..6266)
                     /locus_tag="PLF77_09820"
     CDS             complement(5721..6266)
                     /locus_tag="PLF77_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HPC09193.1"
                     /translation="MGKGIVDRLKKEYPLEDHWVSFELHPETPPAGVLLAERFKDYDL
                     SSFYEQLCQRGKEVSVVFGNQKILSNSRFALMASEYARDLDRYDSFHENMFHAYFTEG
                     LDIGKTDVIAAVAKKSGLDEKGTRNAIHDDRYASRLNEARKEGKSLGLTGIPLFIINN
                     KYQITGAQPIETFRELLKKIT"
BASE COUNT         1562 a         1575 c         1626 g         1762 t
ORIGIN      
        1 tggatgagtc ccaatgaaaa agccaagaag aaggctgccg aactgcttaa gaaaggggcg
       61 atatttgcct tcggtttgtc cgagcggacg cacggcgcgg atatctattc aaccgaaaca
      121 acgctgacgc cggccgataa cggtacctgg gttgccaacg gtgagaaata ttacatcggc
      181 aacggcaatg aagcggaaat ggtttccacc ctgggcaagg tcaaggacgg taccgacgac
      241 tatgttttct tcgtcaccaa ttacaagcac aaagcctatg aattgaagaa gaacgttatt
      301 tctcatcagg aatacgtttc caatttcgcg cttcatgatt acccgattac cgaagacgag
      361 atcctaatgc gcgggcctca cgcctgggat tcggcgctca ataccgtgaa catcggcaaa
      421 ttcaacatcg gtcccgcgtc catcggcgtg gtcgagcatt gcttttatga ggccattacc
      481 cacgcgtcca atcgcatcct ctatggcatg aaggtgacgg atatgccgca tgtgcgtaag
      541 aattttatgg atgcctggct caggctggtg gcgatgaagc tgtatcagag gcgttccaca
      601 gactacttcc gcaacgccaa tgacaaagac cggcgctatc ttctttacaa tcccaccagc
      661 aagatgaagg ttaccctgca gtcggaagat gttttaaatc ttttatggga ggttattgcc
      721 gccaagggct ttgaaaaaga cacctacttc catatggccg ccgccgatat ccgggggccg
      781 tccaaactgg aaggaacggt gcacgtgaac gttcaactca tccgcaaatt catgaaaaat
      841 tatttcttca accccgtgtc ctatgctccg gtcgctcccg atttctctgc taaagatgat
      901 ttgtttctgt tcaatcaggg accgaccaaa ggcctgggat ctgtgcagtt tcacgattac
      961 aagccggttt ttgaagcggg aagaaagctg cccaatgtgg agattttcat ccggcaaatc
     1021 aatatcttta aggaaatgct tgaaaaagcg ggtcctgaca aggcgcagga tttggatccc
     1081 tcctggtcac tgccggtggg cgaaatgttc tccatcgtgg tgtacggaca gctcatttta
     1141 gagcaggctg ccttcgacaa ggtggatgat gatatcctca atcagatttt cgatttcatg
     1201 gtgcgcgact ttgcccgctt tgccctgcag atctacggta tgcacaatac caaagaagac
     1261 cagcgcgcct actgcaaaga cattatgctg attaaaggcc agggcgacga tgcccagtat
     1321 gacagggtct ggcagaaata cgttgccgtt ctgaacggcg aatacgcgat gagtgaatag
     1381 gctgcacaaa cggccgaaga cgttcggaca ttggattgaa gggcgaagca caatggcttc
     1441 gccctttttt tttcaaacgg cgcagttgtt ttgacggaaa cgcgaggcgg gatcctgccc
     1501 gtaatattcc ctgagtctcc ggtagagttc cggggcgttt ttttccataa tgagcggttg
     1561 ttcaaagaaa tgttccgtgg ccacggcgaa gaattccgct tcattggtgg cgccgtaggc
     1621 gtcgagaaac gtctgtctgc ctttctcggt gtcgcgtctg agacgaagat attcgtgtga
     1681 aaatgttttg atccagtcgc ggtatttggc gcggtctttc agcggcgggg tcccgtcggc
     1741 tgcgccgtca agcatatcca gcttatgggc gaactcgtga taaacgacat tatgtcccga
     1801 ttcggactga cggccaccct gcaacgcggc gtcccagaca atgatgaccg gcccctgttg
     1861 aaaggcttct ccgacaatcg gataattttc ttccagcggc tccagcgaga cttcgaaaaa
     1921 accgggtttg cgccgaggcg ctaccaccgt ggaggggtaa acaatgacgg tttcaacatt
     1981 gcggtaatag ttatgggaca atctcagcag gagcaggcag gcgtgagccg caatggtcac
     2041 gcggatttca tcgttcagaa caagccctcc cgcgccctcc cagtgttttt cagcaatgaa
     2101 cacctgtatc agcgaacgca gatgagcgcg ttctgcatca tggagcagac ggtagtgtcc
     2161 catattgcgc tcgagaatgt cctcccaggc atcgggaaat ggcgctgccg taagcttgct
     2221 gcgtcggcga ttgatgaacc attgaaacaa atggggttcc ttttacttca agtcaataaa
     2281 aaatattttg gcaaatgaat agatccctct ggtgtttctg aaatggaagg ttcacactga
     2341 ttcaaagaaa aatcaagggc gaaaagcctt ctgttcgatg gaagtctttt cgccctgaga
     2401 aggcaatctt ttttaaacca tgtcgatctt tatttgccca gcatctgact tttcaatgcc
     2461 ttgtcagccg gtttgttgca cagccatatg ccgaaaagca cttttttgaa atccagccct
     2521 ttggtggtgg tcttcaactg tttgtttttg tatacctgcg ttccttctgc aggggtgtat
     2581 acgaggtcgt aaacgtcatt gatcttgatg ttgtccgcaa acacagagat gaacgtatcg
     2641 attttgtctt tatagggcgc gatattgccg ttggtggaat tctggaatcc ttccatggtg
     2701 gcgtctttca tcttatcgct ggtgatcagt ttggaaatga tgtggattct gacggccatg
     2761 ggttcatcgg cgttcagtat cgcgttggca tcgctgtttt ttttcttgag gtacagcccg
     2821 gtgacatacg ctttgatgaa gaatttcgtt cgtatgccgg cgccgttcag gatcagcgtg
     2881 tcagtgccgg cagcgagagt gtccggcatg ttgatcccgc cgacttcttt tgcatagccc
     2941 acggatgtaa ggataagaac gacagagcac aacagcaata atttcttaaa cataatgaac
     3001 acctctcttt ttttatttta gcccgcttaa ggccaagtga atgctgaagg ataaaatatt
     3061 ccggttccgc ttcgggacct ttctttctgt gcggggagta tagagacaaa gccttctgtc
     3121 tgtcaattat ttatttccat atctttttaa agagattgcc tcgggactgc ggcctgtcgg
     3181 gcaatggaca ggccttccgg cgccgtcaga agcgccggaa ggcgagagga cgtttaagaa
     3241 atttcttcaa tgccgagcgg atatttcgac atgacgtcac agccgtcttt ggtgatgaca
     3301 atatcatttt cataacggaa gccgatatct tcttccggtg cggcgtactg catcgcgcaa
     3361 acgaccattt cattttcctg ataaacatga tcctgatccg tccagtaggg caggtcggga
     3421 ttgacaatag agccgattcg ccattcgtcg ccgaacagac cgatgccgtg ttcgcagccg
     3481 ggcagaataa aatcagcgca gccgcgggat tccgcgaagt ccatcatcat ggcggccagt
     3541 tttccgggcg tggttcccgc gcggtaatta tcgatgactt tccggatgat cgagacaaaa
     3601 ttatccgcgt gccagcgctg acgttttgtt gcgggatcga tgagataatt gtgcgagtga
     3661 tcgccaagcg ccagcttgaa catggcgtga aagtccagca tcagcggttc gcccgctttg
     3721 atttctttcg ttgtcggcgg tgtgcatgcg cccaggccgg tgcggtaacc gctggagatt
     3781 tcgttgagcc ccgcaaaatt ccattccgat acgctgcccg ctttgcgcat ggccagcgcg
     3841 gcaataccgg cgataacagt ttccgtcatg cccctgtagc cgccgttttc gagcgcggcc
     3901 cgcgccgctt tgtggccttc atccacaatc agtgacgccg tgcggaagcg ttcaatcgtt
     3961 cccttgtctt ttatcaggga aagtccgtcg atgatatcgt gcgcgtttac ccattcaaaa
     4021 tccggaagag catccctgag acgggtatat tcccagtgcg acaaatttcc ttcggcagta
     4081 taggtggaaa cgccgtcctc atatcccagc cgtccttttt tgatgccgag ttcgtcctta
     4141 atgtaatcgg tgatggcgga cacctgatcc atgccgccca tcgggccgta gccgcggaca
     4201 tgatcgctgt ccagccaggt ttcatcgccc acgcgcagcg cgtcgataac aaatgtaaaa
     4261 aaagccggtt gtccctcgga cgggataatc aaatagctgc gccagggaca aaaggcatcc
     4321 aaaacgaaac tcatcgtgcg gatgcgtgaa ccgaggtaaa cgtcgatgcc gcgtttgtgc
     4381 atttcagcct gaatggcttt gacgcgtccg gggttgtcga tataatattt atcgttggga
     4441 tcgaatattc tgggcatgat ttctctcctt atccatattg ggttggaatt tgggccagga
     4501 ctttattcag cctgtccgat acagctgcga gcttgagcgc cttcgccaga ttccccttga
     4561 gtttgatttt tccggtcatc agcgccttct gagagccgag ttccgcgcgg gaaattctgg
     4621 aaaacgtttc ataatcgccg atgatgagaa attcagcctt gggcgggtcg ccgactccgg
     4681 tctccacacg ggttatgttt ccctccttgc attcgatgaa taaaaacatt tctttcccgc
     4741 cggggcagtt cttgtaaatg ttggacatcg atgtggaaac cttgttcatt ttatccggtg
     4801 taagttccgt ctgaagtcgc ttaaatgctt cgtcgcacca tgccgggctt aagtaggcaa
     4861 gtaattctga catgctgata cctcctgcgt tgtctgattc cggacgtcat gaacatgaca
     4921 tccggatggg taccccttta caggtgtttt tcgcttctcg ccaaaaagtt gacggagcac
     4981 tttctcatca atataaaatt ataggggcaa agaagaggta agtcaatgtc aataaacctc
     5041 aaggatacca aaagcttgtc atacaaacac agcctcaggc gtccgccata accatgcttg
     5101 ctgatggtga cctgaccgca gccttcaggt tgttggagtt gtgaaaacgc aaacccgttt
     5161 ttttattttt ctatttgcgt tgccagcaat tcgagaagct ctttgttttc cggaatgtcc
     5221 atcatcgaat ggccgtaatg gacaaaacgg actttgccgg atttgtcgat gatgatctgc
     5281 gcgggcattc tgcccagctt gaatatcttg acctcctggc cgtacaattt caacacatga
     5341 tgttcgggat ctggcaggcc tacaaagggc agcttttctt tcacccagta ttttttgaaa
     5401 gcttcggcat cttccggtcc tgcaaccaga atttccgtat ccagtttgac aaacttttcg
     5461 taatcctggc gcaactgcgc catgtgcacg cggcagaacg gtcagataaa accgcggttg
     5521 aaaaccagca tcacattctt tttgttttga aaatcggaaa gcgaaacgat ggtgccattg
     5581 aaatccttca acgtaaaatc cggcgcttga acatttaatt ccactcgggc cataagttaa
     5641 cctccttaac tatttttaaa tgatacgctg aaattttcaa ttttcaattc gtttttaacg
     5701 taaggatagg cgtatatatc ttaagtaatt tttttcaata gctcacggaa ggtttcaatc
     5761 ggctgagcac cggtgatctg gtatttgtta ttgatgatga agaggggaat gcctgtgagg
     5821 ccaagcgatt ttccttcctt tctggcttcg ttcaggcgtg acgcataacg gtcatcgtgg
     5881 atggcgttgc gtgttccctt ttcgtcaagc ccgctttttt tcgcaacagc ggcaataaca
     5941 tccgttttgc caatatcgag accttcagta aaataagcgt gaaacatatt ttcatgaaac
     6001 gaatcgtatc ggtccagatc gcgggcatat tcggaagcca ttagtgcgaa tcttgaatta
     6061 gaaaggattt tttgattgcc aaacacaaca cttacctctt tgccgcgttg gcacagttgc
     6121 tcataaaatg aagaaagatc ataatccttg aatctttcgg caagcagcac tcctgccggc
     6181 ggcgtttccg gatgaagctc gaagctcacc cagtgatcct ccaagggata ttcttttttc
     6241 agccggtcga caatgccctt gccgatgtag cagaagggtc agatatagtc ggaatatatt
     6301 ttgaggtgga ttgtcatttt atgcctcgaa attttcgtta aatatactcg catttatcgt
     6361 aattttacat cgttttttaa aaaagaaata ttgcaatatc cgatgaaaaa acgtaagctc
     6421 aactttcaaa aaaaatgttt tgccttcgat aaaatttgcg ctgtaaatgc cctgtggttt
     6481 ttggattggt ttgatcggta ccgcggaagc ctgtgttcca tgtgc
//
LOCUS       DAOOLB010000104         6451 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_14320_length_6451_cov_50.517667, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000104 DAOOLB010000000
VERSION     DAOOLB010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6451
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14320_length_6451_cov_50.517667"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="PLF77_09825"
     CDS             complement(<1..431)
                     /locus_tag="PLF77_09825"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta"
                     /protein_id="HPC09194.1"
                     /translation="MLNLVVCLKQVPMVTELPWDEKTGTLRRDLAAGMMNPACKHALE
                     AALQIKEHHPANITVITMGPPAAEEVLRESLALGADQAVLVCDRLLAGSDTFATSLIL
                     KEAIKKKCPKFDLILCGAYTADSETAQVGPQLAEELDLPAAA"
     gene            complement(434..1585)
                     /locus_tag="PLF77_09830"
     CDS             complement(434..1585)
                     /locus_tag="PLF77_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPC09195.1"
                     /translation="MQLNPCAKHKLIRDSVRTFAETELAPVASAIDRAATFPREIIEK
                     MRPLNYFGLQVPREFGGAALDSVSAAIVVEEISRVCAAIGLCVTVHNGVAVYPFLRFA
                     NAEQKKHYLPKLAEGELIGAFSLTEANAGSDSASVETTAVKRNDAYVLNGTKIFVTNG
                     GVCDLALIFALTGSEQGKPQSSVFIVESKYPGFLRGELEDLCGMRANPVSSLFLEDCC
                     VPENNVLGRIGDGMKIGLATLDNGRIGVAAQALGIAQGAFEAAVKYAKERQQFKKPIA
                     SLQTIQNYIADMATEIAAARLLLYRACSLKDEGMPFGCEASMAKLYCSTVASRVTSLA
                     VQIHGGYGYSKEYDVERYFRDAKVTEIYEGTSEIQRMVIARSILSQLIL"
     gene            1842..3110
                     /locus_tag="PLF77_09835"
     CDS             1842..3110
                     /locus_tag="PLF77_09835"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HPC09196.1"
                     /translation="MANPQEKLAVSLPLRLIIRLALGFVDLFHIWRFPRKTDGCFEHM
                     TLIDKLHWLYKSANPIRLAEKKSSLEDFFAGQAETSLTLPPDFEQTTQIQLAAVGDLM
                     QNDLLTHSADSLYEGIAAELFTADISFANLEAPLTTQPQRNLLFVRSETPSTYISAGQ
                     FQVLKGYKDQKFTVFSTAGNHAWDYGEEGVLSTLDALAKEGIVAVGTNREKADRQKAA
                     IIDVQGIRIGFVGLTFGLNGKKIDPEQEYHVNLVQLNALSAEVDLSVPIRQINYCREQ
                     YCDFIVGSLHWGLEFEFFPARRQLAVAHQLAEAGLDLIISHHPHVVQPVEFYRTRRDP
                     QRTVVITYSLGNMVSPFHDDDLNLSFLLQVTLADGKLKNQKRTYIAACRIIPVCQRAL
                     MAGGKPTVRLEKVTRLAAFRLPFDLKNLAP"
     gene            3285..3785
                     /locus_tag="PLF77_09840"
     CDS             3285..3785
                     /locus_tag="PLF77_09840"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HPC09197.1"
                     /translation="MVIIYPMSKEKIPTTPAVLALKAQQADFTLHTYPYEERGGTKAS
                     ARKLGVDEHMVIKTLVMEDETGKPLIILMHGDREVSTKSLARQIGAKSVTPCKPAVAE
                     KHTGYKVGGTSPFGTRKVLPVYMEQTIADLPDILINAGSRGLLARMPSAELARLLKPV
                     MVGVAI"
     gene            3835..>4121
                     /locus_tag="PLF77_09845"
     CDS             3835..>4121
                     /locus_tag="PLF77_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09198.1"
                     /translation="MPDDVIIRCLNCGTKNRIPKQKLQGRSICGKCGASLDELIIRCL
                     KCSTKNRIPEDRLNQRPLCGKCHEPLIIPQQKVLPFDVTDQTFAREVLTSDS"
     assembly_gap    4122..4221
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4222..4773
                     /locus_tag="PLF77_09850"
     CDS             <4222..4773
                     /locus_tag="PLF77_09850"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPC09199.1"
                     /translation="PCGVFMADNKSSIVYQCKIFDVREEEVTFPNGKTARQSWINHHP
                     TVAIVAVNEKNELLLIKQYRNAVKQDLLEIPAGSLDGIEESPAVCAQRELAEETGFKA
                     KSLIKLYEGYLLPGYCNEYMYFFLALDLFSAPLNPDDDEFIETIPATFSQAHKLIDRG
                     VIIDAKTVLGILLAEKLLGRQNA"
     gene            4874..6172
                     /locus_tag="PLF77_09855"
     CDS             4874..6172
                     /locus_tag="PLF77_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HPC09200.1"
                     /translation="MIYNEEFETMPREVIKALQVKRLQQVLERVYHTVGFYKKSFDAA
                     KVKPDDIRCMADMKKLPFTTRKDLRDNYPFGLFAVPMSSVVRLHASSGTSRRSAVFGY
                     TKRDIDTWSDLIARSLVAAGITKNDIIHNAFGYGLFTGGLGLHYGAEKIGASVIPISG
                     GDVKRQLMILQDFGPTVLCATPSYALHLAEEGNAMGIDIKALKLRVGIFGAEPWTDAI
                     RDDIEKAFGITALDLFGLSEVIGPGMAMECLEGRHGMHIFEDHFIVETIDPQTGDVLP
                     EGAEGELVFTTLTKEANPLVRYRTGDISRLITEPCRCGRTHIKMERVLKRSDDMLIIR
                     GVNVFPSQIEAILVEIEGLEPNYQVIIDKAGALDVLDLQVEVNDKIFSDSGSIKQLQK
                     IEQRIVKDMRDYLGVSARVKLVQPNTLKKTDAKIIDKRRI"
     gene            6195..>6451
                     /locus_tag="PLF77_09860"
     CDS             6195..>6451
                     /locus_tag="PLF77_09860"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HPC09201.1"
                     /translation="MKVEQISVFLENKPGTLEDATRVLKEANINIRTLSVAETVDFGI
                     LRLIVNDVEKANKVLKDNGFRVSKTPVVAVEVPDTPGGLHS"
BASE COUNT         1597 a         1765 c         1549 g         1440 t
ORIGIN      
        1 gccgccgcgg gtaaatcaag ctcctcggcc agttgcggtc cgacctgggc cgtttcgcta
       61 tcggccgtgt aggctccgca aaggatcaaa tcaaatttcg gacatttctt tttgatcgcc
      121 tcttttaaaa taagggacgt ggcaaacgta tccgatccgg ccaacagcct gtcgcaaacc
      181 agcacggctt gatcggcgcc caacgccagc gattcccgaa gcacttcctc ggcggccggc
      241 ggtcccatcg tgattacggt gatattggcg ggatggtgtt ccttgatttg cagagccgct
      301 tccagggcgt gtttacaggc cggattcatc atgccggcgg caaggtcgcg ccgcagcgtt
      361 cccgtctttt catcccacgg cagttcggtt accatgggaa cctgttttaa acaaacgact
      421 aaattgagca tgtttataat atcaactgag acagtatgga tcgcgcgata accatgcgct
      481 gaatttcgct ggtaccttcg taaatttccg taaccttcgc atcgcggaaa tacctttcga
      541 catcgtattc cttgctgtat ccgtaaccgc cgtgaatctg cacagccaga gacgtcaccc
      601 tggatgcgac cgtactgcaa tacaactttg ccatggacgc ttcacagcca aaaggcattc
      661 cctcatcctt caacgagcag gcgcgataca aaagcaggcg cgcagcggcg atttcggtgg
      721 ccatatcggc aatataattc tggatggttt gcagcgaagc gatgggtttt ttaaactgct
      781 gtctttcttt ggcatattta accgccgctt caaaagcacc ctgagcgatc cccagcgcct
      841 gcgcggcaac gccgatgcgg ccgttatcca gcgtagccaa acctattttc atgccgtcgc
      901 cgatcctccc cagcacattg ttctccggca cacagcaatc ttcaagaaaa agcgacgaaa
      961 caggattggc gcgcataccg cagagatctt ccagttcgcc gcgtaaaaat ccgggatatt
     1021 tgctttcgac gataaaaacg ctgctttgcg gcttgccctg ttcagagccg gtcagcgcaa
     1081 aaatcaaagc cagatcgcag acgccgccgt tggtcacaaa tattttcgtc ccgttcaaaa
     1141 cgtacgcatc atttcttttg accgccgtcg tttccacgct tgcggaatcg gacccggcgt
     1201 tggcttccgt cagactgaat gcgccgatca actcgccttc ggccagtttg ggcagatagt
     1261 gtttcttctg ctctgcgttc gcaaaccgta aaaagggata tacggcaaca ccattatgca
     1321 ccgtcacgca caaaccgatc gcggcgcaaa cacgggagat ttcttccaca acaatagcgg
     1381 cgctgacgga atccagggcg gctccgccga attcccgcgg cacctgcagg ccgaaataat
     1441 tcaacggcct cattttctcg atgatttcac ggggaaaagt tgcagcgcgg tctattgcgg
     1501 acgcaacagg cgcaagttcc gtctctgcaa acgttctcac ggaatcgcga atcagtttat
     1561 gcttggcgca gggattcagc tgcaatgttc tctcctggaa gaaaatgctt tgcacaaata
     1621 acttaaaata accgtaaaat caagaaaacc ttattccgaa ggtctttgcc cgtttccgtg
     1681 caccggtact aatcctcagg gttatcaaac gcatgatatg tcattgacag ccgctacggt
     1741 tgactctata cttctatgct atggtacacc gcgaggccaa aaataaatca agccggaaga
     1801 ccaaagcagt ccatcatgac ccggacgctc ttccctcgtt aatggccaat cctcaggaaa
     1861 aactcgctgt ttcgctccct ttacggctta tcatccgact ggctttagga tttgtcgatc
     1921 tttttcatat ttggcgtttt ccccgcaaga ccgacggttg cttcgaacac atgaccctca
     1981 tcgataagtt gcactggctt tacaaatcgg caaacccgat ccgtttagcc gaaaaaaaat
     2041 cttctcttga ggactttttc gccggacagg cggaaaccag ccttacgctg ccgccggatt
     2101 ttgaacaaac cactcaaata caactggctg ccgtgggcga tctgatgcag aacgatctgc
     2161 tgacgcattc ggcggattcg ctctatgaag gcattgccgc cgaattattt actgcggata
     2221 tctcctttgc caatctggaa gctcccttga ccactcagcc gcaacgcaat ttattattcg
     2281 tccgcagtga gaccccgtca acctacattt cggccgggca atttcaggtg ctcaaaggat
     2341 acaaagatca gaaattcacc gtcttctcaa ctgccggcaa ccatgcctgg gactatggcg
     2401 aggagggcgt gctcagcacc ctcgacgctc tggcaaaaga agggattgtc gccgttggaa
     2461 cgaatcgtga aaaagcggat cgacagaagg cggcgatcat tgatgtccaa ggcatcagaa
     2521 taggctttgt cggattaacc ttcggcctca acggcaagaa aatcgatccg gaacaggaat
     2581 atcatgtcaa tttggttcaa ctgaatgctt tatctgcgga ggtcgatctg tctgtgccca
     2641 tccggcaaat caactactgc cgggaacagt actgcgattt tatcgtcggg tccctccact
     2701 ggggtcttga atttgaattt tttcccgccc gacgccagtt ggccgttgct catcaactgg
     2761 ccgaagcggg gctcgatctg atcatctctc accatccgca tgttgtccaa cctgttgagt
     2821 tttaccggac ccggcgcgat ccgcaacgga cggtggtcat tacctattcc ctcggtaaca
     2881 tggtcagtcc tttccacgac gacgacctta acctgagttt tcttctgcag gtgaccctcg
     2941 ccgacgggaa attgaaaaat caaaagcgaa cctatattgc cgcctgccgc attattcccg
     3001 tctgtcagag agcattgatg gccggcggaa agccgacggt gcggctggaa aaggtaaccc
     3061 gtttggccgc attccggctg ccgtttgacc tcaagaatct tgctccataa aaattccgca
     3121 tgaggtcaag cgatgacatt ttgtcgctgc cgattgtccg gagccgtggg atttgctatg
     3181 tcagcggctt cgccgcttgc aatgacacgc tatccgttca cggtacaaca acctttcttc
     3241 atcaacccct taatgcactc ttctctgttg atttaaaacg tttaatggtt attatatacc
     3301 cgatgagcaa ggaaaagatt ccaacaacac cggcggtttt agccctcaaa gcacaacagg
     3361 ctgatttcac tctgcatacg tacccctatg aggaaagggg aggaacaaaa gcatccgccc
     3421 ggaaactggg cgttgacgaa cacatggtca ttaaaacgct ggtaatggag gatgaaacgg
     3481 gtaaaccgct gatcattctg atgcatggcg atcgggaagt gtccaccaag tccctggcca
     3541 ggcagatcgg agccaaatcc gtcaccccat gcaagccggc cgtcgcggaa aaacataccg
     3601 gttataaagt cggcgggaca tcgccgttcg ggaccagaaa agtgttgccc gtgtacatgg
     3661 aacagaccat cgccgatttg ccggatattt taatcaacgc gggcagccgc ggtctgctgg
     3721 caaggatgcc ttcggccgaa ctggcgcgtc tgcttaagcc ggtcatggtc ggcgttgcca
     3781 tatgatttgc ctttttcggg caaaaaagag ataatgcctt tgaggataaa tgctatgcct
     3841 gatgacgtga ttatccgctg tcttaactgc ggaaccaaaa acagaatacc gaaacaaaaa
     3901 ctgcagggac gatcgatctg cggcaagtgc ggcgcttcgc tggatgaact catcatccgc
     3961 tgcctgaaat gcagcacgaa gaacagaata cccgaggacc gattaaatca acggccttta
     4021 tgcggcaaat gccatgaacc gttaatcatt cctcagcaaa aagttttgcc gttcgatgtc
     4081 acagaccaga cctttgcccg cgaagtgttg acgtccgact cnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nccctgtgga gtattcatgg ccgacaacaa atcctctatc
     4261 gtctaccaat gcaaaatttt tgatgtccgg gaagaggagg tcaccttccc aaacggcaaa
     4321 acggccagac aaagctggat caaccatcac cccacggtgg ccattgttgc tgtcaatgaa
     4381 aaaaatgagc tgctgctcat caaacagtat cgcaacgccg tgaaacagga ccttttggaa
     4441 ataccggccg gatcgctcga tgggatcgaa gaatctcctg cggtttgcgc gcaacgggag
     4501 ctggccgaag aaaccggttt caaagccaaa tcgctgatca agctctacga aggctatctg
     4561 ctgccgggtt actgcaacga atacatgtat ttttttctgg cccttgatct tttcagcgca
     4621 ccgcttaatc ccgacgacga cgagttcatt gaaaccatac ccgccacgtt ttcccaagca
     4681 cacaagctca tcgacagagg cgtcatcatc gacgccaaaa ccgttctggg cattttgctg
     4741 gctgaaaaac ttttagggcg gcaaaacgcc tgatggattc ttgtttcccg agaaagcttc
     4801 ttgccagaga caaaaagttt tgttaggctt ttacacgatt ccgatatccg ttaatccatg
     4861 aggaggctgc gccatgatct acaacgaaga atttgaaacc atgccccgcg aagtcatcaa
     4921 agccctgcag gttaaaagac tgcagcaggt tctggagcgt gtctatcaca ccgtcggctt
     4981 ttacaaaaaa tcctttgatg ccgccaaagt gaaacccgat gacattcgct gcatggccga
     5041 catgaaaaag cttccgttca ccaccagaaa agatttgcgc gacaactatc ccttcggttt
     5101 gttcgccgtg cccatgagca gcgtggtccg tctgcacgca tcgtccggaa ccagccgccg
     5161 ctctgccgtt ttcggctaca ccaaacgcga catcgacacc tggtcggatc tgatcgcgcg
     5221 ctcgctggtc gccgctggca tcaccaaaaa cgacatcatc cataacgctt tcggctacgg
     5281 cctcttcact ggcggactgg gactgcatta cggcgcggaa aaaatcggcg ccagcgtcat
     5341 tcccatctcc ggcggagacg tcaaacgaca gttgatgatt ctgcaggatt tcggtcccac
     5401 cgtgctatgc gcgacgccct cctatgccct gcatctggcc gaagaaggca acgccatggg
     5461 aattgacatc aaagcgctga aactgcgcgt gggcattttc ggagccgaac cctggaccga
     5521 cgccatccgc gatgacatag aaaaagcatt cggcattacc gcgctggatt tgttcggact
     5581 gtcggaagtg atcggaccgg gcatggcgat ggaatgcctg gaaggccgcc acggcatgca
     5641 cattttcgaa gaccacttca tcgttgaaac cattgatccg caaacaggcg atgtgcttcc
     5701 cgaaggggcc gagggcgaac tggttttcac cacgctgacc aaagaagcca atccgctggt
     5761 ccgttaccgc accggcgaca tatcgcgtct gattaccgaa ccgtgccgct gcggccggac
     5821 ccacatcaaa atggaacgag tcctgaagcg tagcgacgac atgctgatta tccgcggcgt
     5881 caatgttttc ccgtcgcaga tcgaagccat tctggtggag atcgaaggtc tcgagcccaa
     5941 ttatcaggtc atcatcgata aagcaggggc gctggatgtc ctggatttgc aggtcgaagt
     6001 caacgacaaa atcttcagcg actcgggcag catcaaacaa ttgcagaaaa tcgaacaaag
     6061 aatcgtcaag gacatgagag attatctggg tgtttccgca cgcgtcaagc ttgttcagcc
     6121 caacacgttg aagaaaaccg acgccaaaat tatcgacaaa cgccgtattt gattaaccac
     6181 aaaagggggt tgccatgaaa gtagaacaaa tatccgtatt tctcgaaaac aaacccggca
     6241 cgctggaaga cgcaacgcgc gttctgaaag aagccaatat caatatccgc acattgtccg
     6301 tagcggaaac cgtcgatttc ggcattctgc gcctgatcgt caatgacgtc gaaaaagcca
     6361 acaaagtcct caaagacaac ggtttccgtg taagcaagac ccccgtcgtc gccgtggaag
     6421 tccccgacac accgggcgga ctgcacagca t
//
LOCUS       DAOOLB010000105         6260 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_14858_length_6260_cov_49.287349, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000105 DAOOLB010000000
VERSION     DAOOLB010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6260
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14858_length_6260_cov_49.287349"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /gene="trmFO"
                     /locus_tag="PLF77_09865"
     CDS             complement(<1..1058)
                     /gene="trmFO"
                     /locus_tag="PLF77_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HPC09202.1"
                     /translation="MDHRVTIIGGGLAGCEAAWQLLRRGHAVEMYEMKPQKFSEAHKM
                     ENLAELVCSNSLKSNSLDNAPGLLKEEMRLLDSVIMKAADKTAVPAGSALAVDRLLFA
                     REVETRLCEQTAFTLHRREMTAIPDDTVTIIATGPLTSDAMAQDISRRLGGASLYFYD
                     AIAPIVEADSIDMNKVFWASRYDKGTPDYLNCPLTREEYESFHKALLAGEKVAARDFE
                     ETRHFESCLPIEILAQRGEKSLAFGPMKPVGLIDPHTGNKPYAVVQLRRENAAGTLFN
                     LVGFQTKLTWPEQRRIFRLIPGLEKVEFARYGSIHRNTYINSPALLSPTLQLKNHENI
                     FFAGQITGVEGYMESAAMG"
     gene            complement(1058..3400)
                     /gene="topA"
                     /locus_tag="PLF77_09870"
     CDS             complement(1058..3400)
                     /gene="topA"
                     /locus_tag="PLF77_09870"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPC09203.1"
                     /translation="MANSLVIVESPTKVKTIKKFLGNDFNAVASMGHIKDLPKSKLGI
                     DLEKDFEPSYSIIETKKKTIDELKKAAKNAENIYLAPDPDREGEAIAWHINEVLNTKN
                     KNVYRVLFNDLTKDTVTEAIKNPLQLDFNKYEAQQTRRILDRLVGYQISPVLWDKVKR
                     GLSAGRVQSVAVRMICDREDEIKNFKPEEYWNLTAKFEGKNPPPFEAKLHKVDGKKAK
                     VTNGEQAAMLADKIKQAEFFVEKLEKKEVKRTAPPPFTTSKLQQEASRWFRFSAKKTM
                     MVAQKLYEGIELGKEGSVGLITYMRTDSYRISEEAIKDVRDYIRESYSADYLPHKPHT
                     YKNTQKAQDAHEAIRPSRMTNKPQDIKQYLSADQFKLYQLIWNRFVASQMNPAILDQT
                     TIDIAGANCLFRAQGQIMKFPGFTIVYTEGKDDKDENGNGNGNGTDKFLPEVSEKEKL
                     KLLHLNTEQKFTQPPPRFSEASLVRELEEKGIGRPSTYAAILSTIQEREYVKLEKGKF
                     YPSELGMMVTHLLVKSFPTILEIAFTADMENKLDAIETGERKRVQTLKEFYALFADEL
                     KKAKAEMKNIKQEETPTDLVCEKCQSPMVIRWGKNGKFLCCSNYPKCKNTMNFTHDEN
                     GKVKHVETRTTDIKCNKCGKNMIVKEGRFGQFLACSGYPECKNTMTAAKDENGEIVAQ
                     ATPTTDEVCELCGKPMTVKRGRYGQFLGCTGYPECKNIKKLDKDGKAAKQEVVLSDEV
                     CELCGKPMAVKRGRYGQFLGCTGYPECKNIKKIPKTKTES"
     gene            complement(3526..4656)
                     /gene="dprA"
                     /locus_tag="PLF77_09875"
     CDS             complement(3526..4656)
                     /gene="dprA"
                     /locus_tag="PLF77_09875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPC09204.1"
                     /translation="MNDEILKYWIALKSIKGIGHVSFPALVDRFGSPAAVFGASVSDL
                     QQTEGISRQIAEAIVHFRGWDNVKAELELIRKNDVTIVTYEDELYPGQLLNIYDRPPY
                     IYVRGNLSREDINLAIVGSRAASTYGKYTTERISRDLALQGVTVVSGMARGIDSSAHR
                     GAITAHGRTVAILGSGLDVIYPPENKKLFADIVENGAVVSEFPLGTPPRSTNFPARNR
                     IISGISYGVVIVEAGEKSGSLITARLALEQGREVFAVPGSIDSAGSRGTNKLIKQGAK
                     LIENTEDILEEILPQIEQRKPLKGFSAPGTLNAASPPMEKLNAAEQKLYDLVSQKRMH
                     IDDLISVTGFSSADILSALTTMELKGIVRQHPGKFFSAERTN"
     gene            4864..>6260
                     /locus_tag="PLF77_09880"
     CDS             4864..>6260
                     /locus_tag="PLF77_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09205.1"
                     /translation="MFKVKNILIFFITIFFIYLPAGFAQNVTKTQKPSVAPKQGVQKS
                     VDQATKKPKPGLTIMPAGINLGLVAFDKSVEATVSLKNTGTGAIRWSIQGPKSWDKVK
                     SKAISGTLEKETDVLNVGVSLLGNDEFKDEKKAGHSLANVEMRIASGEEEIICTKKLP
                     LGIHKEEIEINAADGQKKITLSFFIAYTQSAPRINLSTLRLDMGKILPNKTVSRKIIL
                     NNSGKETLTWSVEPPAHAREDMPDDFRQGRYLSFMNDEVKGAAQYSVPEPLKDTLELI
                     GSWSETGGYPTSGPGDNLIKVNFTGTGILLYLSEHSREGKLTLALDKNPIARAALFEE
                     QRKNGEEILIADQLEFGSHTLTITNKYEGLVVEGVKILGLNTAFFPAQSIKIFPASGA
                     TTLQTNYLTVLFNTGQAFPGFYEDYLVFKTNNGEAIVEMYAEVLPDVTSAIIDVYRYY
                     NGNDYLFTSNPQAEI"
BASE COUNT         1476 a         1530 c         1565 g         1689 t
ORIGIN      
        1 cccatggctg ccgactccat atagccttcc acgcccgtga tctgtccggc aaagaaaata
       61 ttttcatggt ttttgagctg cagggtaggg ctcagcaggg cgggggagtt gatataggtg
      121 ttgcggtgaa tgctgccgta gcgggcaaat tcaaccttct ccagtccggg aatcaggcga
      181 aaaatccgcc gctgttccgg ccaggtcaat ttggtctgga aaccgaccag attaaaaaga
      241 gtgccggcgg cgttctctct gcgcaattgc acaacggcgt agggtttgtt gccggtatga
      301 ggatcaatca ggcccaccgg tttcatcggg ccgaatgcca gactcttttc gccgcgctga
      361 gccaggattt ctatcggaag gcagctctcg aaatggcggg tttcctcaaa atctctggcg
      421 gccacctttt ctcccgctag caacgccttg tgaaatgatt cgtattcttc gcgggtcaga
      481 gggcaattca ggtagtcggg cgttcccttg tcatagcgcg acgcccagaa caccttgttc
      541 atatcaatgg aatccgcttc gacaatgggc gcaatggcat cgtaaaaata gagcgacgca
      601 ccgccgagcc tccgggaaat atcctgcgcc attgcgtcgg aggtcagcgg gccggtggca
      661 atgatcgtta cggtatcgtc cgggatggcg gtcatttcgc gccggtgaag ggtgaaggct
      721 gtttgttcac agaggcgggt ttccacctcg cgggcgaaca gaaggcggtc aaccgccaga
      781 gcggatccgg cgggcacggc ggttttatcc gccgctttca taatgaccga gtccagcagg
      841 cgcatttctt ccttgagcaa accgggcgca ttatcgagag agttggactt gagggaattg
      901 ctgcaaacca gttcggcgag attctccatt ttgtgggctt cggaaaattt ctgcggtttc
      961 atttcataca tctcaaccgc atggccgcgc cgcagcagct gccaggccgc ctcgcagccg
     1021 gccagtccgc cgccgataat tgtcacacga tgatccatca ggattctgtt tttgttttag
     1081 ggattttttt gatgttcttg cattcgggat aaccggtgca gccgaggaac tggccgtagc
     1141 ggccgcgctt gacggccatg ggtttgccgc acagctcgca gacttcatcc gacagaacga
     1201 cctcctgttt ggccgccttt ccgtccttgt ccagcttttt gatgtttttg cattcgggat
     1261 aaccggtgca gccgaggaac tggccatagc ggccgcgctt gaccgtcatg ggtttgccgc
     1321 acagctcgca gacttcatcg gtcgtcggcg ttgcctgtgc gacgatttcg ccgttttcgt
     1381 ctttggccgc ggtcatcgtg tttttgcatt cgggataacc ggagcaggcg aggaactgac
     1441 cgaaacgccc ctctttgaca atcatatttt tgccgcactt gttgcatttg atatctgtcg
     1501 ttctggtttc cacgtgtttg acctttccgt tttcgtcatg agtaaaattc atggtgtttt
     1561 tgcatttggg ataattggaa cagcacagaa acttcccgtt ttttccccag cgaatcacca
     1621 tgggggactg gcatttctcg cagaccagat cggttggcgt ttcttcctgc ttgatgtttt
     1681 tcatttccgc cttggctttt ttcagctcgt cggcaaaaag cgcgtaaaat tccttgagcg
     1741 tctgcacacg cttgcgttcg cccgtttcaa tggcgtccag tttgttttcc atatccgcgg
     1801 tgaaagcgat ttccagaatg gtcggaaaac tttttaccag aagatgcgtc accatcattc
     1861 ccagttccga cggataaaac ttgccttttt ccagcttcac gtattcgcgt tcctgtattg
     1921 tggaaagaat cgcggcatag gtgctggggc gtccaatgcc tttttcctcc agttcgcgca
     1981 ccagcgacgc ttcggaaaaa cgcggcggcg gttgtgtgaa tttctgttcc gtattcagat
     2041 ggagaagttt caacttttct ttttcgctga cttccggcaa aaacttgtcc gttccgttgc
     2101 catttccgtt gccgttttca tccttgtcat ccttgccttc cgtatagacg atggtgaagc
     2161 cggggaattt catgatctgt ccctgcgcgc ggaaaagaca attggcgccg gcaatgtcaa
     2221 tggtggtctg atcgagaatg gccggattca tctgactggc gacaaagcgg ttccagatca
     2281 gttgataaag tttgaattga tcggcgctca gatattgctt gatgtcctgc ggtttattag
     2341 tcatgcggga cggacgaatc gcttcatggg catcctgcgc tttttgcgta tttttgtacg
     2401 tatgaggctt atgcggcaga taatccgcgg aataactttc tctgatgtag tcgcgaacat
     2461 ctttgatggc ttcttcggat atccggtagg agtccgttct catatatgtg atgagaccca
     2521 cggaaccttc cttgcccagt tcgattcctt catagagttt ttgcgcgacc atcatggtct
     2581 tcttggctga aaaacgaaac cagcgcgatg cttcctgctg caatttactg gtggtaaacg
     2641 ggggcggcgc agtgcgcttg acttctttct tttccagttt ttcaacaaaa aactccgcct
     2701 gcttgatttt atcggcaagc atcgcggcct gttcgccgtt ggtcactttt gcttttttgc
     2761 cgtcgacttt atgaagtttc gcttcaaaag gaggcggatt tttaccttcg aatttggccg
     2821 tcagattcca gtattcttcg ggcttgaaat tttttatttc gtcctcccgg tcgcagatca
     2881 tccggacggc gacggactga acgcgccccg cgctcaagcc tcgtttgacc ttgtcccaca
     2941 gcaccggact tatttgataa ccgaccagac ggtccagaat gcggcgggtc tgctgcgctt
     3001 catatttgtt gaaatccagt tgcaggggat ttttgatggc ctcggtcacg gtatctttgg
     3061 ttaaatcgtt gaagaggacg cggtagacgt ttttgttctt ggtgttgaga acttcattaa
     3121 tatgccaggc aatggcttcg ccttcgcggt ccgggtcggg ggccagataa atgttctcgg
     3181 cgttcttcgc cgcttttttc agttcatcga tggttttctt tttcgtttcg atgatgctgt
     3241 acgacggctc gaaatctttt tccagatcaa tacccagttt gcttttcggc aaatccttaa
     3301 tatgacccat cgaagcgacg gcattaaaat cgtttccgag aaattttttt atcgttttaa
     3361 cttttgtggg agactccaca atcaccaaag aatttgccat aatcccattc tctcttaaaa
     3421 aatagaaagc ccctataaat ggaagaaaaa aatttgtaaa gctatttttt acgatcttcc
     3481 tttcatttca ttatttaaat atccggaaaa taatagtttt attgattagt ttgttctttc
     3541 tgcagaaaaa aattttcccg ggtgctgccg gacaattcct ttgagttcca tggtggtcaa
     3601 agcgctgaga atatcggcgg acgaaaatcc cgtcacagaa atcaaatcgt cgatgtgcat
     3661 gcgtttttgg gaaaccaaat cgtataattt ctgctcggcg gcatttaatt tttccatggg
     3721 agggcttgca gcgtttaatg tcccgggagc ggaaaatcct tttagcggtt ttctctgttc
     3781 tatctgcggc agaatttctt ccagaatatc ctctgtgttt tcgatgagct tggctccctg
     3841 tttgatcagc ttattcgtac cgcgcgaacc ggcggaatca atgcttcccg gtacggcgaa
     3901 aacttcacgt ccctgttcca gcgccagcct cgccgtaatc aacgagccgc ttttttcgcc
     3961 tgcttccaca atgacaacgc cgtaggatat gccgctgata atgcggttgc gcgccgggaa
     4021 atttgtcgaa cgcggcggcg ttcccagggg gaattcggaa accacggcgc cgttttcgac
     4081 aatgtccgca aataattttt tattttccgg aggataaatg acatccaggc cgctgcccag
     4141 aatcgcaacg gttctgccat gagcggttat agcgccgcga tgcgccgacg aatcgatgcc
     4201 ccgagccatc ccgctgacca ccgtcacgcc ctgaagggcc aggtcccggc tgattctttc
     4261 cgtcgtgtat ttaccgtaag tgctcgctgc acgggaaccg acaatggcaa gattgatgtc
     4321 ttctctgctc aaatttcccc gcacatatat ataaggaggg cggtcataga tattcaataa
     4381 ttgccccgga tagagctcat cttcgtaagt aactattgta acgtcatttt tgcgaataag
     4441 ttcaagttct gctttaacat tatcccagcc tctgaaatga acaatggcct ctgcaatctg
     4501 acgggaaatt ccctctgttt gctgcaggtc ggaaacggaa gcaccgaaaa cagcggcagg
     4561 agagccgaac ctgtccacca gtgccggaaa agagacgtgg ccgatgcctt tgattgattt
     4621 taacgcaatc cagtacttta gaatctcatc attcataaat gacccgaacg agaagcgtta
     4681 tggtgatgat tggggatttt gaaaaattga cagcaaccta catcttgtct gccggatgtg
     4741 tcaagtattt tcaaaatgat gatccgccgg atgaacaaag agtgaaaaat gtgaaattct
     4801 taaaacagtt gcttaatttt aagctttgag ctaggaaacc atcaacgttt catagaggtc
     4861 gtcatgttca aggtaaaaaa tattctaatt ttttttatca caattttctt tatttacctg
     4921 cccgccggtt ttgctcaaaa cgtcacaaaa acgcagaaac ctagcgttgc gcccaaacag
     4981 ggtgttcaaa agtcagtgga ccaggcaacg aaaaaaccaa aaccgggcct gaccattatg
     5041 ccggcgggaa ttaatttggg tcttgtcgcc tttgacaaat cggtggaagc aaccgtcagt
     5101 ttaaaaaata cgggtaccgg cgcgatccgc tggtccatcc aggggcctaa gagctgggac
     5161 aaagtaaaat ccaaagcgat atcgggaaca ctggagaagg aaaccgatgt tttaaatgtg
     5221 ggtgtaagtt tactgggcaa tgatgaattc aaagacgaaa aaaaagccgg gcattcgctg
     5281 gcgaatgtcg aaatgagaat tgcctccgga gaggaagaaa ttatctgtac caagaaactt
     5341 ccgctaggaa tacataaaga ggaaattgaa atcaacgctg cggacgggca gaaaaaaata
     5401 accttgtctt ttttcattgc ctacacgcag agcgctcccc ggatcaacct cagtacgctg
     5461 agattggata tgggaaaaat cctgccgaat aaaacagttt ccagaaaaat aatattaaac
     5521 aacagcggta aggaaacgtt gacgtggtcg gtcgaaccgc cggcacacgc ccgggaagac
     5581 atgccggatg atttcaggca gggaaggtac ctgtccttca tgaatgatga ggtcaaaggc
     5641 gccgcacaat acagcgttcc cgagcctttg aaagacacac tcgagttaat cggaagctgg
     5701 tcggaaaccg gcggctatcc aaccagcggg ccgggcgata atctgatcaa agtcaatttt
     5761 accggaacgg ggatcctttt atacctgtcc gaacattccc gggaaggaaa actgaccctg
     5821 gcgctggata aaaatccaat cgcgagggcc gccctgtttg aagagcagag aaagaacggg
     5881 gaagaaatcc tgatagccga tcagctggaa tttggttccc ataccctcac gatcacgaac
     5941 aaatatgaag gtctggtcgt ggaaggcgta aaaatactgg gactgaacac agcctttttc
     6001 cccgctcaga gcataaaaat ctttcctgcg tccggggcca ccaccctgca gaccaattat
     6061 ctgaccgttt tatttaatac cgggcaggct ttcccgggct tttatgaaga ctacctggtc
     6121 tttaaaacta ataatggcga ggcgattgtc gagatgtatg cggaagtgct gccggatgtc
     6181 acctcggcga tcatcgatgt ttacagatat tacaacggaa acgactatct tttcacatcc
     6241 aatccgcagg cggaaatcaa
//
LOCUS       DAOOLB010000106         6142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_15258_length_6142_cov_39.880565, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000106 DAOOLB010000000
VERSION     DAOOLB010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6142
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15258_length_6142_cov_39.880565"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="PLF77_09885"
     CDS             <1..615
                     /locus_tag="PLF77_09885"
                     /inference="COORDINATES: protein motif:HMM:NF028222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HPC09206.1"
                     /translation="STFIEPLACVVRAQRLARVKKGQTVLVIGCGMSGLLNVKLAVTK
                     GCRIIASDINRKKLDIALEMGAHIAINAADDISKRLVDQTGRKADIVFLCASADAAVE
                     QAWQCVDKGGVIVLFAVPAPDKKVVVPVNDFWMKEIAILTSYYCGPPDIIEAMKLIEW
                     HHVAIDDLITHRLPLTEIVKGFQLVADGRESIKVIIKPQEQAAL"
     gene            complement(622..1308)
                     /locus_tag="PLF77_09890"
     CDS             complement(622..1308)
                     /locus_tag="PLF77_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09207.1"
                     /translation="MNLKPIYRLFAVVMISSVFCLSGCAGPRLYSVGMYYDAEQSVIP
                     AYVKNDGQTSDAVVAVSEFADTRQIDDPLVIGRVIQNSGEKTLVFPRNVRATRAVANG
                     IKQYLKKAGYKITEINEPWNLKEDKMPMGKAKILIGGNIEELEIICRKGKLTNSYDTT
                     MTLAVVIADMGQGRILYKTRVKSSYSREHVLFSENILGQQADKVLSDTIEKLFEDQKV
                     AQTIKDVMAR"
     gene            1473..2126
                     /locus_tag="PLF77_09895"
     CDS             1473..2126
                     /locus_tag="PLF77_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09208.1"
                     /translation="MTEATQQALTGLRDLSMIKWYVIPLLAIVFYIYTKEIKDARQNG
                     DWNAVLAGLTIFGVDFFNETWNGWVMYLTQRSAFWTTPGDTALRTLVGWNIEIMFMFL
                     IIGIIFYHTLSESKKDKILGLPEKWFWAISYSIFCVFVECLLNIGGHLVWEYPFWYLS
                     FKGVWLIFLIGYFHFFCFAILVISLKSMKAKLTTVGIIYTVPIIMNVLAFGFWGWNY"
     gene            complement(2130..2666)
                     /locus_tag="PLF77_09900"
     CDS             complement(2130..2666)
                     /locus_tag="PLF77_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HPC09209.1"
                     /translation="MKPIGPLMWEHRLIEKMLTAMMRHMDQVEKTRKVNPLVIDMAVD
                     FIRVYADRTHHGKEEEILFRDLAKKDLPADLKNIMQELLDEHVWGRKTTAALVEAKNK
                     YLQGNQAELFRLIDTARQLGNFYPKHIEKEDKHFFYPCQEYFNKEEQTKMLDEFWEFD
                     RKMIHEKYNKIYDDYSGL"
     assembly_gap    2697..2796
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2797..3638
                     /locus_tag="PLF77_09905"
     CDS             <2797..3638
                     /locus_tag="PLF77_09905"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HPC09210.1"
                     /translation="VIATIFPVYDFARHIGGDKITLAMLLPPGTDAHHYELKPDDIIK
                     VTNADVFLFTNFELEQWAYKIIGASDKNTRLQAVETGNGAMMLRLNKEEDEHEAHPAG
                     FDPHIWLDMDNAQKMVDNITSAFIKKDPRNSDYYLKNAGDYKRQLADMDQKYRTTLAA
                     CKTRTVLHAGHWAFAYLTSRYHLKYIAAYNVFADAEPAPQKIFALIDQIKNENVSHVY
                     YEDMMNPRLAKTIARETGTGLLKLNNGHDVTRTDLEKGETFLALMEKNLVNFQKGLQC
                     PEK"
     gene            3623..4372
                     /locus_tag="PLF77_09910"
     CDS             3623..4372
                     /locus_tag="PLF77_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HPC09211.1"
                     /translation="MSGEIIKVDHLSFCYNGTKVINDISFTVQRGDYLGIAGSNGSGK
                     STLIKNILGILEPHQGHITIFDQPVASFDQWHRIGYLPQRINALHYHFPCTVAEIVQL
                     GIKKRNPDDLKRTLDLMGIAHLSSCLIGELSYGEQQRVMLARALIRKPDLLIFDEPTT
                     ALDPETRDVFYALTAKANEHGTTVVLVTHDTGTIGKYARNLLYLDKKIIFFGTFRDFC
                     ASPDMTGFFGPNSQHMICHQHDQKGPATPWN"
     gene            4363..5190
                     /locus_tag="PLF77_09915"
     CDS             4363..5190
                     /locus_tag="PLF77_09915"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPC09212.1"
                     /translation="MELSGIFQYAFVQRAFLGGILIAAIASILGVFLVLRRFSLIGDG
                     LSHVTFGSVAVVMLLGISPAHMTLAALPLVMLSSLAILKLTGSKRIQGDAAIGIISSF
                     GIAAGIILTSISGGYNLDLFSYLFGNILTVTLTEIFFAIIVFLTVVATVIILYDDLLA
                     ITFDEELARTMGVQTGKINTVLFLLTAVAAVLAMKVAGIMLVSAMLIIPPLTALQLSL
                     SFRMTIFTSIIFSVLSVILGITMAFLLNLPAGGTIVVLNIFFLLCVFFARKLMAFGR"
     gene            5197..6123
                     /locus_tag="PLF77_09920"
     CDS             5197..6123
                     /locus_tag="PLF77_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09213.1"
                     /translation="MTLPFQAYRSSIIFIIALFCGMVFDEGRQAGRILILPALMIILT
                     ITLLRFPRGFFRHPASLLFSSIRGNLLNYLVLGNFILLTGAFLIQKQELWIGLALVAA
                     MPASLEIILLGILLRIEKNYVFTSISGTYLGALLIVPLVSLCFFKYIQINYWNIAVLI
                     LCLIALPLVLSRLIVEKDWDRILRKHENTLIDFCHFIVFYAISANSSMFLKKLSDDLV
                     FISLIALTSTFLFYFLIRRIGLYFHGQENKVNFFILLGTMKDYGLAGGIALTLFNPEV
                     AVPPLVFAVFTFIYMNWLSFKARRIEDDCGDV"
BASE COUNT         1512 a         1478 c         1350 g         1702 t
ORIGIN      
        1 agcaccttca ttgagccgct ggcctgcgtt gtcagagcgc aacggctggc ccgcgtaaaa
       61 aaaggacaga ccgttctggt aatcggctgc ggcatgtccg gcctgctcaa cgtaaaactg
      121 gccgtcacca aaggctgccg gattatcgcc tccgacatta accgcaagaa actcgatatc
      181 gccctggaaa tgggagccca tattgctatc aacgcagccg atgatatttc aaaacggttg
      241 gtagatcaga ccggcagaaa ggccgatatt gtttttctgt gtgcttccgc cgatgcggcg
      301 gtggaacagg catggcaatg cgttgacaaa ggcggcgtca tcgttttgtt cgccgtcccc
      361 gcaccggata aaaaagtggt tgtaccggtc aacgacttct ggatgaagga aattgccatc
      421 ctgacgtcgt attactgcgg tcctcccgac attattgaag caatgaaact gatagagtgg
      481 catcatgtcg ccatcgatga tctgatcacg caccggctgc cgctgacgga gatcgtaaag
      541 ggctttcaac tggttgccga cggcagggaa tccatcaaag tcatcattaa accgcaggag
      601 caggcagctc tttaatgtcc attaacgggc catgacatcc ttaatggtct gcgctacctt
      661 ttgatcctca aaaagctttt caatggtatc ggacagaacc ttgtccgcct gctgtccgag
      721 aatattttcg gaaaacaaaa catgttcccg gctgtaagaa ctcttcaccc gcgttttgta
      781 cagtatcctg ccttggccca tatccgcgat aacgacggcc agcgtcatcg tcgtatcgta
      841 ggaattcgtc agttttcctt tccggcagat gatttccagt tcttctatat taccaccaat
      901 cagaattttg gccttgccca tcggcatttt atcttctttc aaattccacg gttcattgat
      961 ttcggtaatt ttatacccgg cttttttcaa atattgcttt atcccgttcg caacagccct
     1021 cgttgctctg acattgcggg ggaacaccag ggttttttcg ccgctgtttt gaatcacgcg
     1081 gccgatgacc agcgggtcgt caatctgacg tgtatcggca aattcggaaa cggccacaac
     1141 agcgtcggat gtctgcccgt catttttcac atacgcgggg atcaccgatt gctctgcgtc
     1201 ataatacata ccgacactgt agagtctggg tccggcgcag ccggatagac aaaaaacaga
     1261 agaaatcatt acgacggcga aaagacgata tatcggctta agattcataa acactccctt
     1321 cattatgatc aatcaggatc ataaaccctg acttttttct ttttattttt gaaagatata
     1381 taatataatt ataaaagttt agcaaagtaa aagatgggaa gcattgacaa ccttaaccga
     1441 ttttttaact caaaaaattt aaggagggca ttatgacaga agcaacccag caagctttaa
     1501 ccggcctgag agatctttcc atgatcaaat ggtatgtaat tcctcttttg gctatcgtct
     1561 tttacattta caccaaggaa atcaaagatg cacgacaaaa cggagactgg aatgcggttc
     1621 tcgccggcct gactattttc ggcgtggatt tcttcaacga aacatggaac ggctgggtca
     1681 tgtacctgac gcagcgctcc gcgttctgga caacccccgg cgataccgcc ttgcggacac
     1741 tggtcggatg gaacattgaa atcatgttca tgttcttaat catcggaatt attttctatc
     1801 atacgttatc cgaaagcaaa aaagataaaa tcctggggct gcctgaaaaa tggttctggg
     1861 caatcagcta cagcatcttt tgcgtttttg tcgaatgcct gctcaacatc ggaggccatc
     1921 tggtctggga ataccctttc tggtatctgt cctttaaagg cgtctggctc atttttctga
     1981 tcggctattt ccactttttc tgctttgcca ttctggtcat cagcctgaaa tccatgaagg
     2041 ccaaactgac gaccgtcggc atcatctaca ctgtgccgat tattatgaac gtactggcat
     2101 ttggattctg ggggtggaat tattagccgc taaagcccgg aatagtcgtc ataaatttta
     2161 ttatattttt cgtgaatcat tttgcggtcg aattcccaga attcgtcaag catcttggtt
     2221 tgctcttcct tgttgaagta ttcctgacag ggataaaaga agtgtttgtc ttccttttcg
     2281 atatgcttgg gataaaaatt gcccagttgt cttgccgtat caatcaggcg gaataattcg
     2341 gcctgattgc cctgcaaata tttatttttt gcctccacca gcgcagccgt tgtctttctg
     2401 ccccagacat gttcgtcaag cagttcctgc atgatgttct tcaaatcggc aggaagatct
     2461 ttttttgcca gatcccggaa aagaatctcc tcttccttgc cgtgatgcgt ccggtcggca
     2521 tagacgcgga taaaatcaac ggccatatca ataaccagcg gattcacctt ccgggtcttt
     2581 tccacctgat ccatatggcg catcattgca gtcagcattt tttcgattaa ccggtgttcc
     2641 cacatcaaag gtccgatggg tttcataggt ctgcctcctt tttttgaaca tgatatnnnn
     2701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnatgt tattgccaca atttttcccg
     2821 tttacgattt tgcgcgccat atcggcggtg ataaaatcac cctcgccatg ctcttgcctc
     2881 cgggaacaga cgcgcatcat tacgaactga agccggatga tatcatcaaa gtcaccaacg
     2941 cggatgtttt tctctttacg aattttgaat tggagcagtg ggcctataaa atcatcggcg
     3001 cctccgataa aaacacccgt ctgcaggcgg tggaaaccgg gaacggcgcc atgatgctga
     3061 ggctgaacaa ggaagaagac gaacatgagg cacatcctgc cggctttgat ccgcatatct
     3121 ggctggatat ggataacgct caaaaaatgg tggacaatat aacgagtgcg tttattaaaa
     3181 aagatcctcg caacagtgac tattacttga aaaatgccgg tgactacaaa cgccagttgg
     3241 cggacatgga tcaaaaatac cggacaacac tggccgcctg taaaacgaga acggttctgc
     3301 atgcgggcca ctgggcattt gcctatctga cgtcacgata tcatctgaaa tacattgcgg
     3361 cctataatgt tttcgctgat gcagaaccgg caccgcagaa aatctttgcc ctaatcgatc
     3421 agatcaaaaa tgaaaacgta tcccacgtct attatgaaga catgatgaat ccgcgtctgg
     3481 caaaaaccat tgcccgagaa acgggcaccg gtctgctgaa actcaataac ggacatgatg
     3541 tcaccagaac ggatctggaa aaaggagaaa cctttcttgc tttaatggaa aaaaatctcg
     3601 tgaattttca aaaaggatta caatgtccgg agaaataatc aaggtcgatc atctctcgtt
     3661 ctgttacaac ggaaccaaag taatcaacga tatttccttt accgtccaga gaggagacta
     3721 tctgggcatc gccggttcca acggatccgg caaaagcact ctgatcaaaa atatactggg
     3781 catccttgaa cctcaccagg gacatataac aatctttgat cagcccgttg catcctttga
     3841 tcagtggcac agaatcggtt acctgccgca aagaatcaac gcgctgcatt accatttccc
     3901 ctgcacggtc gcggaaatcg tacaactcgg catcaagaaa aggaatccgg atgatcttaa
     3961 aagaaccctt gatctgatgg gtatcgcgca tctttcctcc tgcctcattg gagaactatc
     4021 ctacggtgaa cagcaacggg tcatgctcgc ccgggctttg atcagaaaac cggatttgct
     4081 gatttttgat gaaccgacaa ccgctttgga tccggaaacg agagatgttt tctacgccct
     4141 gaccgcgaag gccaatgagc atggaacgac ggttgtactt gttactcatg acacggggac
     4201 gattggaaaa tatgcccgca atcttctcta cctcgacaag aaaataatct ttttcggaac
     4261 cttcagggac ttttgcgcat cgccggatat gacgggattt ttcggaccga acagccagca
     4321 catgatttgc catcaacacg atcagaaagg acccgcaacg ccatggaact gagcggcatt
     4381 tttcagtatg cattcgttca gagagccttt ctgggaggca tactcattgc ggcgatagct
     4441 tccatcctgg gcgtatttct tgttctgcgc cgcttttccc tgatcggcga cggtctttca
     4501 cacgtcacgt tcggaagtgt cgccgtcgtc atgctgctgg gtatcagtcc cgcgcatatg
     4561 acgctggcgg cgctcccgct ggtgatgctg tcgtcgctgg ccattctgaa actgacaggt
     4621 tcaaaacgga ttcagggcga cgcggccatc ggaatcattt cttccttcgg tattgctgca
     4681 ggcattatcc tgaccagtat ctccggcgga tacaaccttg atcttttcag ctatctgttc
     4741 ggcaacattc tgaccgtaac cctaacggaa atcttttttg ccatcatcgt ttttctgacc
     4801 gtcgtggcga cagtgatcat cctgtacgat gatcttctgg ccatcacatt cgatgaagaa
     4861 ctggcccgga ccatgggcgt tcaaaccgga aaaattaata cggttctctt tctgctcaca
     4921 gcggttgcgg cggttttagc catgaaagtc gccggtatta tgctcgtttc ggccatgctg
     4981 atcatccccc cgctgaccgc cctgcaactg tctttaagtt tcagaatgac catttttacg
     5041 tcaattattt tctcagtatt atcggtcata ttgggaatta cgatggcctt tttgttgaat
     5101 ttaccggccg gaggaaccat tgtggtctta aacatatttt ttcttttatg tgtttttttc
     5161 gccagaaaat taatggcttt cgggaggtga cgccgaatga cccttccctt tcaggcatac
     5221 cgttcaagca tcattttcat tatcgcgctg ttttgcggta tggttttcga cgaggggcgt
     5281 caggcgggaa gaatacttat tctgccagcc ctgatgatta ttctgaccat tacgttactg
     5341 agatttccac gcggtttttt ccgtcatccc gcttctcttt tgttttcctc catccggggc
     5401 aaccttttga attatctcgt tttgggaaat ttcatccttc taaccggtgc ctttctgatt
     5461 caaaaacagg aactctggat cggcctggcg ctggtcgctg ccatgcctgc atcgctggaa
     5521 attattcttc tgggaatatt actgcgtatt gagaagaatt atgtctttac atcaatatcg
     5581 ggaacctatc tgggtgccct gctgatcgtg cctttagtca gcttatgctt tttcaaatat
     5641 atacagataa actactggaa cattgccgtg cttatcctgt gtctgatcgc cctgccgctg
     5701 gttctttccc ggctgattgt agagaaagat tgggaccgga ttctcaggaa acatgaaaat
     5761 acgctgatcg acttctgtca ttttatcgtt ttttatgcca tttccgcaaa cagcagcatg
     5821 tttttaaaaa agctttcaga cgatcttgtg tttatatccc tgatcgcttt aaccagcact
     5881 tttttatttt attttctcat tagaagaatc ggtctctact ttcacggtca ggaaaacaag
     5941 gtgaattttt tcatcctgct cggaaccatg aaagattacg gactggccgg cgggatagcc
     6001 ctgacccttt ttaatccgga agtcgccgtg ccaccgcttg tttttgccgt atttacgttt
     6061 atttacatga actggctcag tttcaaggca agacgcatag aagacgactg cggcgatgtg
     6121 tgaaaaacct caaatatcaa tt
//
LOCUS       DAOOLB010000107         6077 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_15481_length_6077_cov_36.565095, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000107 DAOOLB010000000
VERSION     DAOOLB010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15481_length_6077_cov_36.565095"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="PLF77_09925"
     CDS             complement(<1..276)
                     /locus_tag="PLF77_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09214.1"
                     /translation="MGDFDKRKYIRYPAEPNELVTVYYMDEKTKKTGQRVGLGENESF
                     KGCCVVFVGDVDFKQGQEVFCECGTLPKTKAQIVWVKKLENQITKVGI"
     gene            complement(437..1168)
                     /locus_tag="PLF77_09930"
     CDS             complement(437..1168)
                     /locus_tag="PLF77_09930"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPC09215.1"
                     /translation="MELNIIRKAMAHKTISLHVLLTVFLVTNYSAITGAAETGKKSLL
                     PDRSVITLPAPVYQGRMSVEEALRKRESVRQFSQSPLTEQELAQLLWAAQGKTRDWAA
                     RTAPSAGALFPLEVYVVIREGVFRYSSGDHRILRVVKTDVRRQLAAAALGQDIIRQAP
                     AVFVIAGVYKRTAEKYSNRAKRYVEIEAGHAGQNLLLQAVSLGLGAVPVGAFQDAHVK
                     QTLHLPVNHEPLYLIPVGRKSSVPP"
     gene            complement(1234..1692)
                     /locus_tag="PLF77_09935"
     CDS             complement(1234..1692)
                     /locus_tag="PLF77_09935"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HPC09216.1"
                     /translation="MPSKKLKEFLNSQSVKYVIIAHSTAYTAQEIAQSAHIPGTELAK
                     TVIVYIDGKMAMAVLPASYSIDFDDLRKETGAKKVELASEAQFKDMFPDCEIGFVPPF
                     GNLYGMDVYVDEHLTRNKEIAFNAGGHLELYRMSYRDFEELVKPKVLKFT"
     gene            complement(1979..2698)
                     /locus_tag="PLF77_09940"
     CDS             complement(1979..2698)
                     /locus_tag="PLF77_09940"
                     /inference="COORDINATES: protein motif:HMM:NF012400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HPC09217.1"
                     /translation="MKMDKETLSKHDGREGHKAYVAVEGRVYDVTGNRLWKNGVHMNR
                     HQAGTDLTEAIAASPHGKDVLSKLQDMGQMSPEKVAKPPFIPLWMHHWLEAYPFFKRH
                     PHPMVVHFPMAIFIIAPLFLAWYYLINPVPGLPAAVFYLYILGTLSLPAAIATGLLAW
                     LVNYSGQPNPLIIRKTIFSLLLLICDGIVMAALIISPAILQDPSGTEWMIPVLIFLGL
                     PLVSIIGEHGGRLVFPVLKSK"
     gene            complement(2779..>3904)
                     /locus_tag="PLF77_09945"
     CDS             complement(2779..>3904)
                     /locus_tag="PLF77_09945"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HPC09218.1"
                     /translation="VKNKKPFLTEVRSQDVLSVSIPDTRSFAGAQRKSFRVWWIAFRY
                     HFVPASFLPAILGSVGAWVQTGLMNRWFFFLTVLGVTLNHIALNMTDDYFDYHAAVDQ
                     GGERRENPYTGGSGTLTGGLIKHGHMRLMFTLFYLITIGIGLYLCAAQTWWIIVFGLF
                     GVGCSYFYTAPPIRYGYRGLGEFSQLINFSFTIGMGAYVVQTVSFSWEIFWILLPLGF
                     MMFAMIVINEIPDRFDDAAAGKNTLVVRFGLKAAARFYGVAMMLAYAVILLAPPLNMA
                     GYWIYLGLLTLPWSVRAFLIMKAYGNEPDKLAPANLLTIRVHNLTGILLIIACLIHGL
                     QNRQVFQPVVFAAVILSAFYLPAAISIFFKKMPDGRRTEA"
     assembly_gap    3905..4004
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4005..4343
                     /locus_tag="PLF77_09950"
     CDS             <4005..4343
                     /locus_tag="PLF77_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HPC09219.1"
                     /translation="GRDGREIYMATFKDTKKYHNLIANPNVSLLIDTRVVDAEGTPRA
                     LTVTGIVEAVDNDKSIEEIRETLLRKHPDLKDFFNHPDARMIVVRPTTLQLLDNVTDA
                     YFEELRQPKR"
     gene            complement(4374..>6077)
                     /locus_tag="PLF77_09955"
     CDS             complement(4374..>6077)
                     /locus_tag="PLF77_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HPC09220.1"
                     /translation="DSIVSEGQLMSALALQKKSGKMIGQTLIELGYITEAQLVEAFRK
                     KFSISYIDCDGVVVPEKFLKLVSMTMAVKKKILPTDVSGNTLFLAMVNPLDYSTIDEV
                     KFLTGKNITPLLTTESSLMKAIEKNYGYCSIEKIWKPLNDIHLDGEMQFNLDKSIEFH
                     KMEEEDESALRADDLYKIGEEPPIVKLVTLILVNAVKSRATDIHIEPHEKYVSVRYRI
                     DGDLSTVLKISKTYQEAMTSRIKIISTMNITIRRMPQDGRSTVKYEGRMIDLRVSTIP
                     SAHGETIVIRILDSVKGLIPLSKLGMPDNIIRDLLEMLNKPQGMLFVTGPTGSGKTTT
                     LYSLLTQLSLESESVVTIEDPIEYELENIKQVPVNEKVGFTFAAALRSILRQDPDVIM
                     VGEVRDSETAQISTKAALTGHLVLSSVHTNDTIATISRLRDLGIEPYLISSALNAILA
                     QRLVKKICENCRVEFEIPQYLFENYPYLKKLKIGISYKGKGCEACGFTGYKGRIGIFE
                     FLRVDGNLRRTIPLYRSDQDLCIVARENGMKTLIEDACEKVEHGLTTINEVLTKIHYY
                     E"
BASE COUNT         1488 a         1532 c         1477 g         1480 t
ORIGIN      
        1 gattccgact ttggtaatct ggttttccag ctttttaacc cagacgattt gggcttttgt
       61 tttgggaagc gttccacatt cgcaaaacac ttcctgtccc tgtttgaaat ccacgtcgcc
      121 gacaaaaacg acacagcacc ccttaaaact ttcattctcg cccagcccca cgcgttgtcc
      181 ggtctttttg gttttctcgt ccatgtagta gaccgtaacc agttcgttcg gctccgcggg
      241 ataccggatg tattttctct tgtcgaaatc gcccataaaa tacaccgtcc tgactattta
      301 cagttcaaaa gcattgtaac gacaaatttg atttttggaa aatttcagcg aaaccttttt
      361 ttcaggaaaa gtttatggca gccgcgtttt tctgtcaaga aaatctccga tataaaattt
      421 cgggcaacat ttttttctac gggggaacag aactttttct gcccacgggg atcagataca
      481 acggctcatg attgacgggc agatgcagcg tctgtttgac gtgtgcgtcc tgaaaagcgc
      541 caaccggaac agcgcccaat cccagcgaca cggcctgcag cagaagattc tgaccggcat
      601 gacccgcttc gatctccaca tatcttttgg cgcggtttga gtatttttct gcggttcgtt
      661 tatagacacc ggcaataaca aaaaccgcag gcgcctgccg gatgatgtcc tgaccgagcg
      721 ccgccgcagc caactgccgg cggacgtctg tcttgacgac acgcaaaatc cgatggtctc
      781 cggatgaata acggaagacg ccttcccgga tgacaacata aacctccagg ggaaacaggg
      841 cgcccgccga cggtgcggtc ctagctgccc agtcacgcgt ctttccctgc gccgcccaga
      901 gaagttgggc caattcctgc tccgttaatg ggctttgtga aaattgtctt accgattcac
      961 gttttctcaa agcttcttcc accgacatcc tgccttgata cacgggagcg ggaagcgtga
     1021 tgacagaccg atccggcagg agactctttt ttcctgtctc tgccgcgccg gtgattgcgc
     1081 tgtaattcgt cactaaaaaa accgtcagga ggacgtgaag acttattgtt ttatgcgcca
     1141 tggccttgcg gatgatgttc aactccataa cactttctct cattatttaa taaacggagg
     1201 tttggaagag acggcgaatg gcatcatgtc cgttcaggtg aatttcagca ctttcggttt
     1261 tacgagctct tcgaagtccc gataagacat tctgtacagt tccagatgac cgccggcatt
     1321 gaaggcaatt tccttattgc gggtaagatg ctcatccaca taaacgtcca ttccgtaaag
     1381 attaccgaag ggcggtacga aaccgatttc acaatccgga aacatatcct taaattgcgc
     1441 ttcagacgca agttccactt tctttgcgcc cgtttccttc cggagatcat caaaatcgat
     1501 ggaatagctg gccggcagaa ccgccatggc catcttgccg tctatgtaaa cgataacggt
     1561 cttggccagc tccgttcccg gaatgtgcgc agactgcgca atttcctgtg ccgtgtatgc
     1621 cgtggaatgg gcaatgatga cgtatttcac cgattggctg ttcagaaact ctttgagttt
     1681 ttttgatggc atggcaggcc tccttattat ccttgtgcat gtgggtttgt tatggacttc
     1741 actaacgtac cttgccggaa aaccaggggc gcaaatatcc gaaggagttg taacagggac
     1801 agcgctatat taaaccttat cagtcaataa cacaataaaa agaaaagggc tgttcccggc
     1861 aaaccgccgg caggttgcgg tgaaaaaatc attcgttcat ggtttgatcg gtcaccatca
     1921 aagagacgaa acgatacgtt ataaaccgcc ccgcaatggc atcttccgac gtccgccgct
     1981 atttgctctt cagcaccggg aaaaccagcc tgccgccatg ctcaccgata attgacacca
     2041 gcgggagccc cagaaagatc agaaccggta tcatccattc ggtgccggac ggatcctgca
     2101 ggattgcggg gctgatgatg agcgccgcca tcacgattcc gtcgcaaatc agcagcaaca
     2161 gggaaaatat tgttttgcgt ataatcagag ggttcggctg tcccgaataa ttaaccagcc
     2221 aggccagcag gcctgtcgca atggctgccg gcagcgacag cgtacccaga atgtagagat
     2281 aaaaaacagc agccggcagt ccgggtaccg gattgatcag gtaataccag gcaagaaaaa
     2341 gaggagcgat gataaaaatg gccatgggga aatgaacaac catcggatgc ggatgccgtt
     2401 taaaaaatgg ataggcctca agccaatgat gcatccacag gggaatgaac ggcggcttgg
     2461 ccactttttc agggctcatc tgccccatgt cctgaagttt ggacaaaacg tcctttccat
     2521 gcggcgaggc tgcaatggct tccgtcagat cggtcccggc ctgatgacgg ttcatatgca
     2581 cgccgttttt ccaaaggcgg tttcccgtaa catcatagac cctgccttcg acggcaacat
     2641 acgctttgtg gccttccctg ccgtcatgtt tcgacaaggt ctccttgtcc atcttcatag
     2701 cataccctcc ccgtattaat tataatgatt gtaatgtttt cccgcccggc tttcaagacc
     2761 gaatgcgact ggatattatc atgcttcagt gcggcggcca tccggcattt ttttaaagaa
     2821 aatactgatc gccgccggca gataaaaagc ggacaggatg accgcggcaa aaacaaccgg
     2881 ttgaaacact tgccgattct ggagcccgtg aatcaggcag gcaatgatca acaagattcc
     2941 ggtgagatta tgaacgcgaa tggtcagtaa attcgcaggc gccagtttgt ccggttcgtt
     3001 gccgtatgct ttcatgatta aaaatgcgcg aacggaccac ggcagggtca gcaagcccag
     3061 ataaatccag tagcctgcca tgttcagagg aggtgcaagc agaatgaccg cgtaggccag
     3121 catcattgcc acgccgtaaa accgggctgc cgcctttagg ccgaatcgca ccaccagcgt
     3181 gtttttaccg gccgcggcat cgtcgaatct gtccgggatt tcattgatga cgatcatagc
     3241 aaacatcatg aaccccaggg gcaacaggat ccagaaaatc tcccacgaaa aagaaacagt
     3301 ctgcacgaca taggcgccca tgccgatggt aaagctgaaa ttgatcaact gggagaattc
     3361 tcccaggccg cgatatccgt accggatggg cggtgccgtg taaaaatacg aacaccccac
     3421 gccgaacagg ccgaaaacga tgatccacca ggtctgcgcc gcgcacagat agaggccgat
     3481 cccgatggtg atcaggtaaa ataacgtgaa catcaggcgc atgtgcccgt gtttgatcag
     3541 gccgccggtc agggtcccgc tgccgccggt ataaggattt tcccggcgtt caccgccttg
     3601 atcgacggcg gcgtgataat caaaatagtc gtcggtcatg ttcagggcga tgtgattgag
     3661 cgtaacgccc agcaccgtga gaaagaaaaa ccatcggttc atgagaccgg tctgcaccca
     3721 ggcgccgaca gaccccagga tagccggaag aaaactggcg ggaacaaaat gatagcgaaa
     3781 cgcaatccac cagacacgga acgattttct ttgggctccg gcaaacgacc gggtatcggg
     3841 aatggaaacg gataaaacgt cctgcgacct gacttctgtt aagaaaggtt ttttgttttt
     3901 cacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngggcgg gatggccgtg
     4021 aaatctacat ggccaccttc aaagatacaa aaaaatatca caatctcatc gccaatccca
     4081 atgtcagtct gctgatcgat acgcgtgttg tcgatgccga aggaactccc cgggcattga
     4141 cggtcaccgg catcgttgag gctgtggata acgataaaag tattgaggaa atccgggaaa
     4201 cgcttctccg gaagcatccc gacctgaaag attttttcaa tcatcccgac gcccggatga
     4261 ttgttgtccg gccgaccacc ctgcagttgc tggataatgt gacggatgct tattttgaag
     4321 agctgcgaca gcctaagcgt tgagacgaaa cagaaggttt cccgaaaaaa attctattcg
     4381 tagtagtgta tttttgtcag aacttcattg atcgtggtca aaccgtgttc aaccttttca
     4441 caagcgtctt caatgagggt tttcatgccg ttttcgcggg caacgataca aaggtcctga
     4501 tcggaacgat acagggggat ggtccgtctc agattgccgt caacccgcag gaattcgaaa
     4561 atccctatcc gtcccttata gcccgtaaag ccgcatgctt cgcacccttt ccccttatag
     4621 gatattccga ttttgagctt tttcagataa gggtagttct caaaaagata ctgcggtatt
     4681 tcgaattcca cccggcagtt ttcacatatt tttttcacaa gcctttgtgc aaggatggca
     4741 ttcagagcgg aactgatcag ataaggttct atgcccagat cccttaaacg ggagatggtt
     4801 gcaatggtat cattggtatg gaccgagctg agtaccagat ggccggtcag cgccgccttc
     4861 gtggaaattt gagcggtctc ggaatcccga acttcgccga ccatgatgac gtcgggatcc
     4921 tgcctgagaa tcgaacgcag agccgcggcg aacgtaaacc cgactttttc attgaccggc
     4981 acctgcttga tgttctccag ctcgtattcg atcgggtctt cgatcgtcac gacgctctcc
     5041 gattccagcg aaagctgtgt cagcagggaa taaagcgtgg tggtttttcc gctgcctgtg
     5101 ggaccggtta caaacagcat cccctgaggt ttgttgagca tctccagaag atctctgatg
     5161 atgttatccg gcatccccag cttgctcagc gggatgagtc ccttgaccga atccagaatc
     5221 cgtatgacaa tggtttctcc gtgagccgac gggatggtgg aaacacgcag atcgatcatg
     5281 cgtccttcat atttgacggt actcctgccg tcctgcggca tccggcggat ggtaatgttc
     5341 atggtggata tgatcttgat ccgggaggtc atcgcctcct gatacgtttt ggatattttg
     5401 agaacggtgg acaggtcccc gtctattctg tagcggacac tgacatattt ttcatggggc
     5461 tctatgtgga tgtccgttgc ccttgatttg acggcattga ccagaatcag ggtcaccaat
     5521 ttgactatgg gcggttcttc tcctatttta tagaggtcat cggctctgag cgcgctttca
     5581 tcttcctctt ccattttatg gaactcgata cttttatcca gattgaactg catttcgcca
     5641 tccagatgga tatcgttcaa cggcttccat attttttcga tggagcaata gccgtaattt
     5701 ttctcaatgg ctttcatgag cgacgattct gtggtcagca ggggggtaat attttttccg
     5761 gtgagaaatt tcacttcatc gatggtggaa taatcaagag gattgaccat ggccaggaaa
     5821 agcgtattcc ccgacacatc ggtcggcagg atctttttct taacggccat ggtcatactg
     5881 accagtttaa gaaatttttc cggcacaacc acaccatcac aatcgatata ggaaatagaa
     5941 aatttctttc gaaaagcctc gacaagctgt gcttccgtga tgtagccgag ttcaatgagc
     6001 gtctgaccga tcatcttgcc gcttttcttc tgaagggcaa gcgcactcat caactggcct
     6061 tcggaaacaa tgctgtc
//
LOCUS       DAOOLB010000108         6066 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_15521_length_6066_cov_32.792880, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000108 DAOOLB010000000
VERSION     DAOOLB010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15521_length_6066_cov_32.792880"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="PLF77_09960"
     CDS             <1..788
                     /locus_tag="PLF77_09960"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC09221.1"
                     /translation="KPKTGDRIEEHYSIEGLNIEGNLPVILAQLDSFDRCLRAPDKSG
                     VRNFNLLFYGPPGAGKSELARYVAKRLDRELMVKRASDIVSPYVGETEQNISRVFSEA
                     ESMEAVLVIDEADSFLFSRDRAVRSWEISHTNEFLTQMERFRGILICTTNRFADLDMA
                     SVRRFNQKIGFGCLKPADNMIFYQKLLCGLCKTPVNRNQEELVGRIHDLTPGDFRIVR
                     DRFAISDEKCVTHEQMIQALEAEARVKKTQQGNRPIGFMRRTA"
     gene            1150..1602
                     /locus_tag="PLF77_09965"
     CDS             1150..1602
                     /locus_tag="PLF77_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09222.1"
                     /translation="MDADDIQPLPDECLIYPDTSLSVPVKLAQLVLQIFLIHTPWYIS
                     KRGALIGYDLYDTKQLFGGKVVKAISMTSDRAHYRQNFSMFLDKNRAGIRRTKGILMS
                     VWGLSIMQANEILKETRLLLTAGADELFTCHNLPADEPNILVTLFLAF"
     gene            1872..3167
                     /locus_tag="PLF77_09970"
     CDS             1872..3167
                     /locus_tag="PLF77_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09223.1"
                     /translation="MHKDTDWRIPLKPFLIEPIISFGSSAMKVTGGINWLVMGVLKNK
                     EKFCAVLDLEMRPLFRKCGLANLVKHTELELARREKCDFIQTWHWAANPNFNAAIAPG
                     LKSGFILYHGQPQDGDVYEDRGHVHLRYYFDETKKRSVEVKTKDGKVFMSPIDNHAII
                     DYLEACPNKYPGRMIQSIEEYSKGKAMTPIKKNKRIAEKHNGGSERQRIFIAQGAAGF
                     EYTRQRNTYRIGDSISFYPILQIDHFKKSSQNAPYLKHVVYNIYEFCFKVDAVQRLEE
                     THGMMRNDYLMELLPNFKVCCRDWNKENQFIVDKFYEGYGRLTIGDHRSSMHYKQPPH
                     QQNILMKGKLVGISDDLMQRDFSTKYAHQVKRFRYGKDVYGDEDALEWFGYYRDLKKF
                     KKNVISYTTNPVEYESTDSFALYGYSPKLIFCIEIIAPQ"
     gene            3196..4716
                     /locus_tag="PLF77_09975"
     CDS             3196..4716
                     /locus_tag="PLF77_09975"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HPC09224.1"
                     /translation="MVAIQSKHKEQKSSAKKKAGNYLSKSLYIRGLQCHKSLFLQKYN
                     PTLKDDISDAQEAIFQGGRDVGVLAWQLFPGGIEIPYEGLSHQEQIDLTQSQIKEGRK
                     TIYEATFSHQNVFVKVDILHKGSRGWEIYEVKAGTKLDPVYVDDAALQYHVLTGAGIK
                     VKKVFITHINVEYIRKGTLDVQTLFTSEDITKQVKAKQPFVVKQLRKQRRMLNGELPK
                     IDIGPHCSDPYACDFQEYCWRHIPDNSVFDLRRKGIDPYDLYAQGIIKQKDIPLDLLN
                     EKQRQQVVATINKENTIDKKKIRAFFKTLSYPLYFLDFETFMSVIPPYDGLKPYQNVP
                     FQYSLHFQKKKGGKLFHTEFLAQPGIDPRMPLLKKLLADIPEDVCILTYNMAFEKKVL
                     TELAAQFPVHKKTIGKWINNVRDLMIPFRQRDVYFWKFKGSFSIKKVLPALVPELSYE
                     GLAIADGGAAMDAYQKMLAAMDDQKKLSEIRKNLLAYCQLDTLAMVRILEALEKLE"
     gene            4796..5011
                     /locus_tag="PLF77_09980"
     CDS             4796..5011
                     /locus_tag="PLF77_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09225.1"
                     /translation="MDEFAGTEKYGFRHRKIRHHEAGIAEVCRIFGQEAGAAARQHII
                     SDLKADGWSDGDPFPKDENDYVKIGFF"
     gene            5279..5929
                     /locus_tag="PLF77_09985"
     CDS             5279..5929
                     /locus_tag="PLF77_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09226.1"
                     /translation="MAKKEAFPEGAEPTLLYKDGGKLMEITISDNPGLKKGSAYDENS
                     MLFKLLRRTVIRLKDEFLQVWNGADAEGVLFNLNKFPCYWEMTDEQQEKLHALNKRLM
                     DVEKSIHERYGQIHKDHPEGELSCEIDFLLKEDDPEWSDEDDNIMASMDHSFDEYDPN
                     ASWNDASHLIRDKDGKPLHLCWLFHELYDHLGLVWEDLLRVGQIRVTVQLNYYYHY"
BASE COUNT         1786 a         1259 c         1495 g         1526 t
ORIGIN      
        1 caaagcccaa aaccggcgac cgcattgaag aacattattc catcgaaggt ttgaacatcg
       61 aaggaaatct gccggttatc ctggcgcagc tggactcttt tgaccgctgc ctgcgtgcgc
      121 ctgataagag cggcgtccgc aacttcaatc tcctttttta cggaccgccc ggcgcgggaa
      181 aaagcgaatt ggcccgctat gtcgccaagc gtctggacag agagttaatg gttaagcgcg
      241 ccagtgatat tgtcagccct tatgtaggcg aaacagagca gaatatcagc cgggtgtttt
      301 ccgaggcgga atcaatggaa gcggtgctgg tgattgacga ggcggattct ttccttttca
      361 gccgcgaccg cgctgttcgc tcctgggaaa tcagccatac caacgagttt ttgacgcaga
      421 tggaaagatt ccggggcatt ttgatctgca caaccaaccg ctttgccgat ctggatatgg
      481 cttccgtccg ccgttttaat caaaaaatcg gatttggctg tctgaaacct gcggataata
      541 tgatcttcta ccaaaaattg ctttgcggat tatgcaaaac acccgtgaac aggaaccagg
      601 aagagctggt tggacgtatc catgatctga cgccgggaga cttccgtatt gtgcgagacc
      661 gttttgcgat cagtgatgaa aaatgtgtga cgcacgagca aatgatccag gcgctggagg
      721 cagaagctcg cgtcaaaaaa acacaacaag gcaacaggcc gattggtttt atgcggcgta
      781 ccgcataggc gaatacaaag aagtcaatca acaatcagtt ttatagttct taatcatgcg
      841 ggattattga tggaagaagg caagcatttt atcggtgtgg gtgacaccgg acacagagcg
      901 gcaaaggaat tatgtcggat ttacgaggaa atgcggggat gtctttttga atcagaacag
      961 gacaggccac acaaccattt gcgtattcat tcctatccct gtgccgaaac ggcctgtttt
     1021 gatgataaag atcatcttgt cattctagtg gggtcgctca tcgatccggg tttgcaggat
     1081 gcgcggacgt cttttcatga gagcaggcca tattttatgt tgacggttgg tattgatcat
     1141 cagacaagag tggacgctga cgacattcag ccactacctg atgaatgttt gatttatcct
     1201 gacacgtctc tttctgttcc tgtcaaactg gcgcaactgg ttttgcaaat ctttttgatc
     1261 cacacgccat ggtacataag caaaagaggt gcgctgatcg gttatgatct ttacgacaca
     1321 aagcagttat ttggcggcaa agttgtaaaa gccatatcca tgacatctga tagggcacat
     1381 taccggcaaa acttctccat gtttcttgac aaaaatagag caggcatcag gcggacaaag
     1441 ggaatcctga tgtcggtctg ggggctgtct ataatgcagg caaatgagat tttgaaggaa
     1501 acaagattgc tgctgacggc tggggcagat gaattgttta cgtgtcataa tctgccagcg
     1561 gatgagccga atattttggt aacgctgttt cttgcatttt aaaacgattt tttcggggta
     1621 ctgtactgat gacaacggaa atgatgacag agccaatcat aaaatattgc cgggacaaga
     1681 ttcaacggcg gttgaattta agcattacgc cggacataca gatacggatt agaaattttg
     1741 atggaccgga tcgctgcggt cattacacga tccagccgaa tgcatggaac gtcattgaac
     1801 cggctgaatt ctggtgtgta accggatatt ttaagaaggt tatcgggcag ctccgcaaag
     1861 aataccctca gttgcataag gataccgatt ggcggattcc tctgaagcct ttcctgattg
     1921 aaccgattat cagttttggc agttcggcca tgaaggtcac aggcggcatc aactggcttg
     1981 tcatgggcgt attgaagaac aaagagaaat tctgtgccgt tttagacttg gagatgcgcc
     2041 cgctgtttcg gaaatgtggg ctggcaaatc ttgtgaagca tacggaattg gaattggcca
     2101 ggcgtgagaa atgtgatttc attcagacat ggcattgggc tgccaatcct aattttaatg
     2161 cggccatcgc acccggttta aaaagtggat tcatcctgta tcatgggcaa ccacaggatg
     2221 gcgacgtata tgaagaccgg ggccatgtcc atctgcggta ttactttgat gaaacaaaaa
     2281 aacgctctgt ggaggtaaaa accaaagacg gcaaagtatt catgagtccg atcgataatc
     2341 acgccatcat tgattatctg gaagcttgcc cgaataagta tcccggtcgg atgattcaga
     2401 gcattgagga atacagcaag ggaaaagcaa tgacgccgat taagaagaac aagcgcattg
     2461 cggaaaaaca caacgggggc tcagaacggc aaagaatatt tattgcccaa ggcgcggccg
     2521 gatttgaata tacaagacaa agaaatacat acaggatcgg cgatagcatc tcattttatc
     2581 ccatccttca gattgatcat tttaagaaaa gcagccaaaa tgcgccttac ctgaagcatg
     2641 tcgtctataa tatctatgag ttttgcttca aagtggatgc cgtgcaacga ttggaagaga
     2701 cccacgggat gatgcgcaat gattacctca tggaactgct gcccaacttc aaggtatgct
     2761 gccgcgattg gaataaagag aaccaattca tcgttgataa attctatgag ggatatggca
     2821 ggctgaccat cggtgatcac cgtagctcaa tgcattataa gcagcctccc catcagcaaa
     2881 atattttgat gaagggtaaa ttggtcggta tttcggatga cttgatgcaa agagacttct
     2941 caacgaaata tgcccaccag gttaagcgct tccgttatgg caaagatgtt tatggtgatg
     3001 aggatgctct ggaatggttc ggctattacc gggacttgaa gaaattcaag aaaaacgtga
     3061 tttcttatac aacgaacccg gtggaatatg aatccacaga ttcatttgca ctgtacggct
     3121 acagtccaaa actaattttc tgtattgaga ttatagcgcc acaataaata attcttttca
     3181 aaaggagcaa aaacaatggt agcaattcag tccaaacaca aggagcagaa gtcatccgct
     3241 aaaaagaagg ctggcaacta tctgagtaaa tctctttata tccggggtct gcaatgtcac
     3301 aagtcgcttt ttctacaaaa atataacccc actctgaaag atgacatttc cgatgcacag
     3361 gaagccattt ttcagggtgg aagagacgtc ggtgtcctgg cttggcagtt gtttcctgga
     3421 ggcattgaaa taccttacga aggcttatcc catcaggaac aaattgatct gacgcagagc
     3481 caaattaaag agggaaggaa aaccatctat gaggcaactt tcagccatca gaatgttttt
     3541 gtaaaagtcg atatcctgca caaaggaagt cggggatggg aaatctatga agtcaaagcg
     3601 ggcacaaaac ttgatcctgt ctatgtggat gatgctgcgc tccaatatca tgtgcttacc
     3661 ggcgcgggga tcaaagtcaa aaaggtattc atcacccaca tcaatgtaga atatatcaga
     3721 aaaggaacgt tggatgttca aacgctcttc accagcgagg acataaccaa acaggtaaaa
     3781 gcaaaacagc cgtttgtcgt caaacaattg aggaagcaga ggcgaatgct caatggcgag
     3841 ctacccaaaa tcgatatcgg accgcattgt tctgacccgt atgcatgtga ttttcaggaa
     3901 tattgctggc gacacattcc ggataattct gtatttgatc tgcgccgcaa aggaatagat
     3961 ccttatgatt tatatgcaca gggaataatc aagcaaaaag acatcccgct tgatttgctg
     4021 aatgaaaaac agcgccaaca ggttgtggca acgatcaata aagaaaacac gattgataag
     4081 aaaaagatca gggcattttt caaaacactt tcctatcctc tctactttct tgattttgaa
     4141 acattcatga gcgtcatccc gccttacgat ggccttaaac cctatcaaaa cgttcctttc
     4201 cagtattctc tgcatttcca gaaaaagaaa gggggcaaac tttttcatac tgaatttctg
     4261 gcgcaacccg gaattgaccc gcgtatgccg cttttaaaga aactgctggc cgatataccg
     4321 gaagatgtct gcatcctgac ctacaatatg gcctttgaaa agaaggtgct cacggagttg
     4381 gctgcccagt ttcctgtgca caagaagaca attgggaaat ggataaataa tgttcgagat
     4441 ctgatgatcc ctttccgaca gagggatgtt tacttctgga aattcaaagg atcattttcc
     4501 atcaaaaaag tgttgccggc cctggtgccg gaactttcct acgaaggatt ggcaattgct
     4561 gacggcggcg cggctatgga tgcctatcag aaaatgttgg cggcgatgga cgatcagaaa
     4621 aagctgtcag aaataaggaa gaatctactt gcttactgtc aattggatac gctggcgatg
     4681 gtgcgcattt tagaagcgct tgagaaattg gagtagcgtt atgtcgtgtc ttgaagatca
     4741 ttgtcgggaa tcagtgaacc tcttcggaaa atcttatatg aaggtccatc tgtggttgga
     4801 tgaatttgcc gggactgaga aatacggatt cagacacaga aaaatccgcc atcatgaagc
     4861 cggaatcgct gaagtttgcc gaatcttcgg ccaagaggca ggggcggcgg caagacagca
     4921 tatcatttcc gatttaaaag cagatggatg gagcgatgga gatccgttcc cgaaagacga
     4981 aaatgattat gtgaaaattg gtttctttta gtccgatgaa cgaaatcaga ttttaatatg
     5041 ctggcctcta aaagtttggg aattaatccc gaatcattca tcagccaaga cagcttgaaa
     5101 atgaagagtt caaatagaag ctctgcgcca agagatagct ctgcgcatgt cggagtttca
     5161 ggcaaattaa tcgagaaagg agggatgtgt cattaacaac attatcgctg taaagaacgc
     5221 taagtatctt atgcattgct gtcgcttgtc ccgcatcggc atcccgacgg aattctgggt
     5281 ggcgaaaaag gaagcttttc ccgaaggtgc ggaaccgaca ctgctttaca aagatggcgg
     5341 taagctgatg gaaataacga tctcggataa tcccgggtta aaaaagggaa gcgcatacga
     5401 tgaaaactcc atgctcttca agctgctcag gagaaccgtc atccgcttga aagacgaatt
     5461 tctgcaggta tggaatggcg ctgacgccga gggtgtcttg tttaatttga acaagtttcc
     5521 atgctattgg gaaatgaccg atgagcagca ggaaaaactt catgccctga ataaacgact
     5581 catggacgtg gagaagtcca ttcatgaaag atacgggcaa atacataaag atcacccgga
     5641 aggagagtta tcctgcgaga tagatttctt gctgaaggag gacgacccgg aatggagcga
     5701 tgaggacgac aacattatgg cgagtatgga tcattcattc gatgaatacg accccaacgc
     5761 ctcctggaat gacgcttctc atctgatcag ggataaagat ggaaaaccct tgcatctgtg
     5821 ctggttgttt catgagcttt acgaccattt gggacttgtc tgggaagact tgctccgtgt
     5881 cggacagata cgggtaactg ttcagttaaa ctattattat cattattgaa gccaaggtga
     5941 ggtgttgcat gactaagcaa agaaatccgg atttcagcat ctgtgaaata gcctaccgga
     6001 atgtgcacac tttcttcccg taataattga tttaaccaga gcgtcaaatg tactgacttg
     6061 taggtg
//
LOCUS       DAOOLB010000109         5946 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_15974_length_5946_cov_58.234425, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000109 DAOOLB010000000
VERSION     DAOOLB010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15974_length_5946_cov_58.234425"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="PLF77_09990"
     CDS             <1..624
                     /locus_tag="PLF77_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09227.1"
                     /translation="SYAVLDHRIIGFCAGSLFVHDAIFIYSNDETMVLREFHHRNIAR
                     NMVFCNMRWILKNRHDEGIKHYAFMSISGNPRIVNGYYNHSFLIKILFDCSFKASDKL
                     IRALDAYRHQFKISLVHEDYPFCLKNMFPGSNTFNPSDQRFQFLKRVKRNMPADFDHM
                     ERGDAFAFLVKLPAWFTRTFVFGLMVLAFGRRFFSRRGIGVFAGHNL"
     gene            complement(671..1690)
                     /locus_tag="PLF77_09995"
     CDS             complement(671..1690)
                     /locus_tag="PLF77_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPC09228.1"
                     /translation="MTSPEKIAQPVLVTGATGFIGSRVVQKLLQNETAVKILALPGEA
                     VPAAWAEKIEIVRGSISDPRAVEKAANGAKTIIHLVAIVTDWGDEKEYWEFTVEGSRL
                     IFDEAVKQNARIVLASSIAVYGDNIYRQACPEDTPYGNTLGPYSRTKQAQEKLAWNYY
                     RNKGMKLTVVRPANVYGPRSGPWLHDVVNVLRSGSPGLISGGQMNAGLAYVDNVADIL
                     ILAGTFEKALGNVYNACDGLDVTWKRYFTDIANIIGVGNPKSLPRPLAVLGAYLLEGI
                     YKLLGIKKRPLITREALNLVGSDNRIPIDKIRRELGYEPVISYAEGLKHIREYLHQDS
                     PGKKT"
     gene            complement(1724..>2608)
                     /locus_tag="PLF77_10000"
     CDS             complement(1724..>2608)
                     /locus_tag="PLF77_10000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HPC09229.1"
                     /translation="LDDGPGIRSVVFYKGCPLSCVWCHNPESKKAVEEISFDAKTCID
                     CGACREVCPKNALGRDNPFYIDRSKCDLCFLCLDVCPSGALDSVGKAMSINDIMNKIL
                     PDKPFFDTSGGGVTLSGGEPTLFMDFTSGLLKSLKQNNIHTLLETCGFFDIHKFMDRL
                     YPYLDAIYFDIKIIDREAHKKYCGVSNETILDNFRLLFRQAADDGKMFLARTPLIPDM
                     TDTESNIQGIASFLKSLNITEAALLAYNPLWHEKSDKVGVNDPYKHSKVMTSFRDNAV
                     MERCKTIFGNAGITETKR"
     assembly_gap    2609..2708
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2709..>4001)
                     /locus_tag="PLF77_10005"
     CDS             complement(<2709..>4001)
                     /locus_tag="PLF77_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPC09230.1"
                     /translation="SSDQAITLGGVTPDGEDAVNDMTYIFLKVTEMLSIRDPNVNARY
                     NKSKNSATYLKRLCEVNINTTATPSIHNDEAVMASLAEFNYPPEHLRDWSATGCVEPT
                     LSGRHIGHTNCMMFNMVAALEMALNNGRHPLMRWDLGPKTGDIRAGAFPDFESFFNAF
                     AAQLRFLADQTCEYNNLLGEAHQHIRPTPFISALIEGPLKKGKDVTKGGARYNSSGTA
                     CIGLADITDSLMVVKKLVFEEKKITFERLLDAVNTNFRNDSALLAQIKNKIALFGSGN
                     EEALATANRVIKLAHDIFGEHTNYRNGKYTAGFWSMSNHVAFGTLTGALPSGRLAGKA
                     FTPGLTPEPHASKNILDNIRDVAKLKAENMNNNIAFNVKVVPGAQESHQKVVDNIYSY
                     VKTYSDLGGMQMQLNVVSSKTLRDAMEHPENYQNLLVR"
     assembly_gap    4002..4101
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4102..5111)
                     /locus_tag="PLF77_10010"
     CDS             complement(<4102..5111)
                     /locus_tag="PLF77_10010"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HPC09231.1"
                     /translation="MQNVQPVQKSYANTLARMFFKFVALNFNNRPSLNRYLKSDDGWI
                     NFTFGIKTENGSVEQAMRFENGKVTVLKNIPETLDSQLIFVDENALKEAATQPPNKLM
                     LALMENRMITIGNLGYLQLINLYLSLLVKQIQIAKLKKEAKTENRKYDAAKASRYCIE
                     ARKPRQLKAVNVDPGVRFLNDPYLAQYSLDDFPRVKEFLDIHLKIKPAICHERAELVT
                     QWFKENGFEKDKNGKPWVSELRQAQAFQYLMANKKPIIRKNDLIAGTTTTKDIGVIIY
                     PEAHGTMIWGELLTVPYRPLNPYNIDADTLDVLHNKVFPFWLKRNFREWVRDHKDYPL
                     CQ"
     gene            complement(5145..5816)
                     /locus_tag="PLF77_10015"
     CDS             complement(5145..5816)
                     /locus_tag="PLF77_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC09232.1"
                     /translation="MPKKPRTKEEIENIREKILDTALHIIIEEGFNNLSLRKIASRLN
                     VTATTIYNYYTNKDEINLMIRIRGFEKLHDLLIARSAALKKIEDRLKAMIRAYIEFGL
                     TYPSYYDIMFNLHTPKYLDYAGTEIEPLAFREKQNALKCLTLFIDPVTSYIPIKGKKK
                     EHFILYQVIKFWSDLHGLITLNNSRLFHEVTDDVDDFIAKRTADIVDNFLKLKDSLDE
                     GKKIF"
BASE COUNT         1327 a         1339 c         1442 g         1638 t
ORIGIN      
        1 agttatgccg tgctggatca ccggattatt ggtttttgcg cgggcagtct tttcgtccat
       61 gacgccatct ttatctacag caatgatgag accatggtgc tcagagagtt tcatcaccgt
      121 aatattgcca gaaacatggt cttctgcaat atgcgctgga tcttgaaaaa caggcacgat
      181 gaaggcatca aacactacgc gttcatgtcg atttcaggca atcccagaat cgtgaatgga
      241 tattacaacc atagttttct aataaaaatc ctattcgatt gttcctttaa agcgtcggat
      301 aaattaatcc gcgccctgga cgcctaccgt catcaattca agatatcgct ggtgcatgag
      361 gattatccct tttgtctcaa aaatatgttt ccgggatcga acaccttcaa tccgtcggat
      421 caaagatttc aatttctgaa aagggtcaaa aggaatatgc cggctgactt tgatcacatg
      481 gagcggggag atgcctttgc gtttctggtg aagttgccgg catggtttac acgtacgttt
      541 gtcttcggcc tgatggttct tgcctttggc aggcggtttt tctcccgtcg cggtataggc
      601 gtttttgccg ggcataacct ctgaatcaaa gcgaatgact tcaaaacggt catgactcca
      661 aaaagcgcgt ttaagttttt ttacccggcg agtcttgatg aagatactcc cgaatatgct
      721 ttaatccttc ggcataggaa atgacgggtt catacccgag ctcgcgtcga attttatcga
      781 tcggtatccg gttgtctgat cccaccagat tcaaggcctc gcgcgtaatg agaggacgtt
      841 ttttaatgcc cagcaattta taaattcctt ccagtaaata agctccaagg actgccagcg
      901 gccggggaag ggattttgga ttgcccacgc cgatgatgtt ggcgatgtcc gtaaaatatc
      961 gtttccacgt tacgtccagc ccgtcgcagg cgttgtaaac gttgcccagt gccttttcga
     1021 aagttcccgc aaggatgagg atatccgcca cattatcgac ataggccagt cccgcgttca
     1081 tctgtccgcc ggagatcagg ccgggcgatc cgctgcgaag gacattcacc acgtcgtgaa
     1141 gccaggggcc ggagcgcggc ccgtagacgt tggccggacg aaccacggta agtttcatgc
     1201 ccttgttccg gtaataattc caggcgagct tttcctgcgc ctgttttgtc ctgctgtagg
     1261 gtcccagtgt gttgccgtaa ggtgtatctt ccgggcaggc ctgcctgtaa atgttgtcgc
     1321 cataaacggc tatgcttgag gcgagaacga tccgggcgtt ttgtttcacc gcctcatcaa
     1381 aaatcaaacg gctgccttcg acggtaaatt cccagtattc tttttcatcg ccccagtcgg
     1441 ttacgatagc caccagatga ataatggttt tagctccgtt ggcggccttt tccacagccc
     1501 tgggatcgga gatgcttccc cggacaattt caattttttc cgcccacgca gccggcacgg
     1561 cttcaccggg cagcgccagt attttgacgg cggtttcgtt ttgtaatagt ttctgaacga
     1621 ccctgctgcc gatgaatccc gtggcgccgg tgaccaaaac cggttgtgct attttctctg
     1681 gcgacgtcat tgtgtgattt cctcccgttt atgatacgcg gtattaacgt ttggtttccg
     1741 ttatgcccgc attcccgaag atggttttgc atctttccat gacggcgttg tcgcgaaaag
     1801 acgtcatgac cttgctgtgt ttataaggat cattcacgcc gactttgtcg cttttttcat
     1861 gccataacgg attataagcg agaagagcgg cttcggtgat gttaagactt ttcagaaacg
     1921 acgcaatgcc ctgaatgttg ctttcagtgt ctgtcatatc gggaatcagc ggggtgcgcg
     1981 ccagaaacat ttttccgtcg tccgctgcct gccggaaaag gagtctgaaa ttatccagaa
     2041 tcgtttcgtt ggacacgccg cagtattttt tatgcgcctc acgatcgatg attttgatat
     2101 cgaaataaat agcgtccaga tacggataaa gcctgtccat aaatttatgg atgtcaaaga
     2161 aaccgcaggt ttccaaaagg gtgtgaatgt tgttttgttt gagcgatttc aaaaggccgg
     2221 aggtaaagtc cataaaaagc gtcggctcgc ctccggaaag ggttaccccg cctcccgatg
     2281 tatcgaagaa gggtttatcc ggcaaaattt tattcataat atcgttgatg gacattgctt
     2341 tcccgacgct gtccagcgca cccgaggggc agacatcaag gcacagaaaa cacagatcgc
     2401 atttgctgcg gtcgatgtaa aaaggattat cgcggcccag cgcgtttttc ggacaaacct
     2461 cgcggcaggc tccgcagtcg atacaggttt ttgcgtcaaa ggatatttcc tcgacggctt
     2521 ttttactttc cgggttgtga caccacacgc aggaaagagg gcagcctttg taaaacacga
     2581 cggaacggat acccggtccg tcatccagnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnat gcggacaagc agattctgat aattctccgg atgttccatg gcgtcgcgaa
     2761 gcgtttttga cgacacgacg ttcaactgca tctgcatacc gcccagatcg ctgtaggttt
     2821 tgacgtagga ataaatatta tccacgactt tctgatgcga ctcctgtgca ccggggacga
     2881 ctttgacatt gaaagcgata ttgttgttca tgttttccgc tttgagtttg gccacatccc
     2941 ggatgttgtc cagaatattc ttcgacgcat ggggttccgg ggtgagaccc ggtgtgaagg
     3001 ctttgccggc cagccgtcct gacggaagcg cgccggtcag cgtaccgaaa gcaacgtgat
     3061 tggacatcga ccagaaaccg gctgtgtatt tgccgttgcg gtaatttgtg tgttctccga
     3121 aaatatcgtg agccagtttg atgacgcgat tggctgttgc cagcgcctct tcattgcctg
     3181 atccgaaaag agcaatcttg tttttaatct gagccagcag agcggagtcg tttctgaaat
     3241 ttgtattgac cgcatccagc agccgctcaa acgttatctt cttctcttca aaaaccagct
     3301 ttttgaccac catcagcgag tcggtaatgt cggcaaggcc gatgcaggcc gttccggatg
     3361 aattgtaacg ggccccgccc ttggttacgt cttttccttt tttcaacgga ccttcgatca
     3421 gcgccgagat aaaaggagtc gggcggatgt gttggtgagc ttcgcccagc agattgttgt
     3481 attcacaggt ttgatcggcc agaaaacgga gctgcgctgc aaacgcgttg aagaaggatt
     3541 caaaatcggg gaatgcgccg gcgcggatgt cgcctgtttt cggtcccaga tcccagcgca
     3601 tcaacggatg ccggccgttg ttgagggcca tttccaacgc ggccaccata ttaaacatca
     3661 tgcagttggt gtgaccgata tgccgtccgg acaaggtcgg ttccacgcag ccggttgccg
     3721 accagtcgcg taaatgttcc ggcggataat tgaattccgc aagcgaggcc atgaccgcct
     3781 catcattatg aatggacggg gttgccgttg tgttgatatt gacttcgcaa agccttttca
     3841 agtaggtcgc gctgtttttg ctcttgttgt aacgggcatt gacgttggga tcgcggatgg
     3901 aaagcatttc cgtcactttc agaaaaatgt aggtcatatc gttgacggcg tcttcgccgt
     3961 cgggtgttac gccgcccagc gtaatggcct gatcggagga gnnnnnnnnn nnnnnnnnnn
     4021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nttctggcaa agaggataat ctttatggtc ccgcacccac
     4141 tcgcggaagt tgcgcttgag ccagaacgga aagactttgt tgtgcagaac gtcaagcgta
     4201 tcggcatcaa tattatacgg gttcaacggc ctgtatggaa ctgtgagcaa ctctccccag
     4261 atcatggtgc cgtgcgcctc cggatagata ataacgccaa tgtctttcgt ggtggttgtt
     4321 ccggcgatca gatcattttt cctgatgatt ggttttttgt ttgccatcag atattgaaaa
     4381 gcctgcgcct ggcgcagttc ggaaacccag ggtttgccgt ttttatcttt ttcaaatccg
     4441 ttttctttaa accattgtgt gacaagttcc gcccgttcgt ggcatatggc cggttttatt
     4501 ttcaggtgta tgtcgagaaa ttctttgacc cggggaaaat catcaaggga atattgggcc
     4561 agataggggt cgtttagaaa ccggacgccc ggatcaacgt tgacggcctt gagctggcgg
     4621 ggttttctgg cttcgataca gtatcgggat gctttggctg catcgtattt ccggttttct
     4681 gtcttggctt ctttcttgag ttttgcaatc tggatctgtt tcacaaggag cgaaagatac
     4741 aaattaatca actgcagata gccgaggttc ccaatcgtga tcattctgtt ttccatgagc
     4801 gccaacatca atttgttggg cggttgcgtg gcggcttcct taagggcgtt ttcatccacg
     4861 aatatcaatt gcgaatccag ggtttccgga atatttttca acacggtaac cttgccgttt
     4921 tcgaaacgca tggcctgctc gacgctgccg ttttccgttt tgatgccgaa tgtaaaatta
     4981 atccagccgt cgtcgctttt cagatatcgg ttcagcgacg gacggttgtt gaaattcaag
     5041 gcaacgaatt tgaaaaacat gcgggcaagg gtgttggcat aacttttttg cacaggctgg
     5101 acattttgca taatagacct ccgaataacg ggtttaatga tcagttaaaa aattttcttt
     5161 ccttcatcca ggctgtcctt cagtttcagg aaattgtcta cgatgtccgc cgttcgtttt
     5221 gcaataaagt catccacatc atccgttact tcgtgaaaga ggcggctgtt gttcaacgtg
     5281 ataaggccgt gaaggtcaga ccagaattta attacctgat ataaaataaa atgctccttt
     5341 ttctttccct tgatgggaat gtatgacgtg acggggtcaa taaagagcgt gagacatttt
     5401 aacgcattct gcttttcccg aaaggcgagc ggttcgattt ccgttcccgc atagtccaga
     5461 tacttggggg tatgaagatt gaacatgatg tcgtagtagc tgggataggt gaggccgaat
     5521 tctatgtagg cacggatcat ggcttttaat ctgtcttcta ttttcttcaa ggcagccgag
     5581 cgtgcgatca ggaggtcgtg aagtttctca aagcctctaa ttctgatcat cagattgatt
     5641 tcatctttat tggtataata attgtagatg gtcgtggccg tgacattcaa acgggaagct
     5701 attttgcgaa gactgagatt attaaatcct tcctcaataa taatgtgaag agccgtatcc
     5761 aggatcttct cccgaatatt ttctatttct tctttcgttc tcggtttttt gggcataatt
     5821 tgattcttat ttttaaatat tacgatgtta acttaacagt gttattttaa aaagtcaagc
     5881 gcaatatcaa cgggaccggc agaaattgcc ctgctgcatg atattcatgt aaaatattga
     5941 aataac
//
LOCUS       DAOOLB010000110         5932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_16030_length_5932_cov_46.970393, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000110 DAOOLB010000000
VERSION     DAOOLB010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16030_length_5932_cov_46.970393"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /gene="alr"
                     /locus_tag="PLF77_10020"
     CDS             complement(<1..1430)
                     /gene="alr"
                     /locus_tag="PLF77_10020"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPC09233.1"
                     /translation="MPHKKNQKYRSWVEVDLDNFIANLKEIKRLIGAKADFMMTVKAD
                     AYGHGAIEISNIALKCGARMLGVANADEGVQLRISGIEAPVVILGPSTTAEIEQIIKY
                     NLIPSVSDLSFAKKLNNALIKSHHKRPLHIEVDTGMGRGGAMHTEAVKLVLDLSGLSN
                     IILEGIFSHFASSEKMVSHNSTQWKCFSDVLAEIESRGIYIPIRHINNSGAILNYPEY
                     KLNMVRPGIMAYGIYPSPENESKAGLLPVMSFKTGIVLLKDFPAGHGIGYNSTFVTSR
                     PTRIATIPVGYGDGYPFILSNQSTALIHGQRAPIVGRISMDMCTVDVTHISNCKIGDE
                     VVLLGQQEKECVSANEIAAKAQTISYEILCALGKRAPRVFLQKGKHKTVEPRLKRIFV
                     PEEERSLSRIDNIIRHCLLARARNEELGNAIYYEMFETLFGNRDRRLELRSGFRYDIR
                     IAKPPKMKDAVLSKDYLHVSMHVQYK"
     gene            1704..2015
                     /locus_tag="PLF77_10025"
     CDS             1704..2015
                     /locus_tag="PLF77_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09234.1"
                     /translation="MSRAKVGDVLGCEVCGLKVILDEDCGCATIDLICCEEPMVNLGP
                     AKPKKAQAIAAPRKKTVVKKTKAAPKKKPALKKAKKPTVKKAVKKATVKKTAKKSSKK
                     K"
     gene            2212..>5932
                     /locus_tag="PLF77_10030"
     CDS             2212..>5932
                     /locus_tag="PLF77_10030"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPC09235.1"
                     /translation="MATTKDNPRKKSEELDSFALKVNLERTAATIEIPDQYAPLLKVV
                     ENHYGLQKKIYELLTELHHPFVNWEYVLKELKSVSIGDFYIYNKHQDGLSALATMLCI
                     YFEVIKSSSDKDVKDNAVHYLFDYIDTILNQSNEQTARNLLLFPEIIKSLLDAINQQE
                     IIFKKCSSYAKKIIKNFPGHGDVLQNRLFVDLLYRIFRITYQFWLEQPDPSQWFIAGE
                     GESPESVAAYGQFIRPLSHASMRMLMEKLETSYQNTHEDKNDAIKNYLEMPDYFQIVN
                     GYLQIADQLEKSKTYEGHQHITKLRFLFHVMEVPSLADIHASALREINHSLKMVFQEE
                     QQENLHDFVRKIFGFLKKSKSQREFSGAIFDCITITAREVFAQNSHELANTFISELIA
                     YGFQYPEIKGSNEEWQVKVNPAHIMNIRAWLEIISMKPRWTKKLISALVINLKMGGIF
                     IRDTDLIQKNISALLNTDIAPAYSLIKQLLRIFPVYFSEIGAEGELRDITTKVDELSM
                     RNDKLVYFFRKQSHVESNSLLVKFTEDIFQYWNTGDMSYLRSHLPVEVYEQVMNPGEY
                     FTGMHKAFKYFFTKVQSPLKFLDWDNEKVCKELANVKGINARDQERVKIMIRIYQLIY
                     KKYYPQFIDLLKDLEAVNAFPKADISSLKRSLTTKNYHRSITIILKFLSILKERILSP
                     KKTPSYENIYYKRHIAAGIPSMYGTYYERKFEAVGLSLRLETLATMLFEELSKSFNLK
                     FITKHTIRKIHTYLWLYMNALEIEGIATEGLAAKIKYVTNALSIKQFSLEQYVDIFRF
                     ISKDIQDIIRNYYIDIHTTCLPVVIGQVYRREIDSERKSGQKQHEKLIYAQSENFIRG
                     IIASAFGIQVLDNFVNSVIKTLNAESEKFRDNKQILKVVMAYNPELAVSSFYKKNKKV
                     DNQILLGNKGYFLKELVSFGFNVPPGFIITTEVFRGYEAVYGYKYIFNDLAVRVNKEI
                     KKLEKITGRKLGDRRNPLLLSIRSGATVSLPGMMKSFLNVGINETIAENLSEKDGFKW
                     AAWDSYRRFLQMWGMFKGLNRNFFDAIIDEFKQKYGVERKIQFSPEQMKQIALSYKKG
                     LTDNGIEFKEKPLDQLRDAILQVFDSWYSEQAEIYRRQMHISDKWGTAVIVQKMVFGN
                     LNDNSGSGVIFTREPKSTSSDVTLYGDFIFGVQGDDIVSGLVETYPVSEKQRIAECRD
                     SKISLEDKFPEIYAELLRISEILIYEKGFNHQEIEF"
BASE COUNT         1663 a         1330 c         1386 g         1553 t
ORIGIN      
        1 tttttgtatt ggacgtgcat gctgacatgc agatagtctt tggaaagaac ggcatctttc
       61 attttcggtg gtttcgcgat acgaatatcg tagcggaaac ccgaacgcag ttcgagccgt
      121 cggtccctgt tgccaaaaag ggtttcaaac atttcataat aaatggcgtt gcccagttct
      181 tcgttgcgtg cgcgcgccag aaggcagtga cgaatgatgt tgtcaatacg cgagagggat
      241 cgttcctctt ccgggacaaa tatccgctta agccgcggtt caacggtttt gtgtttccct
      301 ttttgcaaaa aaacacgcgg cgctcttttt cccagggcgc agaggatttc atagctgatc
      361 gtttgtgcct tggcggcaat ttcattggcg ctgacgcatt ctttttcctg ctgccccagc
      421 aaaacgacct catcgccgat tttgcaattg gaaatatggg tgacgtccac cgtgcacata
      481 tccatggaaa tccgtccgac gatgggagcc ctttgaccat gaatgagcgc tgtgctctgg
      541 ttggatagaa taaacggata accgtccccg tagccgacag gaatcgtggc gatgcgggtg
      601 ggccgtgacg taacgaatgt gctgttgtag ccgattccgt gccccgccgg aaagtctttc
      661 agcaaaacga tgccggtttt aaaagacatc accggcaaaa gtccggcttt ggattcgttt
      721 tccggcgaag gatatatgcc gtaggccata attcccggcc gcaccatatt gagtttatat
      781 tcgggataat tcaaaatggc gccgctgttg ttgatgtgac ggatgggaat atatattccc
      841 cgggactcga tttcagccag cacatccgaa aaacatttcc attgcgtgct gttgtgggac
      901 accatttttt cgctggaagc aaagtggctg aaaatgcctt ccagtataat gttggataat
      961 ccggacaaat cgagaacgag ttttacggct tcggtatgca ttgcgccgcc gcgccccatg
     1021 ccggtatcca cttctatgtg caagggacgt ttatgatggg atttgatcag ggcgttattg
     1081 agcttttttg caaaagataa atctgaaacg gaaggtatga gattatattt gatgatctgt
     1141 tcgatttccg cggttgtgga aggtcccagg ataaccacag gcgcttcaat tccgctgatt
     1201 cttaattgca cgccctcgtc ggcgttggct acgcccagca tccgtgcgcc gcatttcagc
     1261 gcgatattcg atatttctat ggcgccgtgt ccataagcat cggctttaac cgtcatcata
     1321 aaatcggctt tggcgccgat tagccttttg atttctttca gattggcgat aaagttgtcg
     1381 agatcaactt ccacccagct gcggtacttt tggttttttt tatgcggcat atgctagtcc
     1441 ggcgtttttc cccctccgaa agcggcttat tgaggatcga ttttttgctg ccctttttgg
     1501 tgatgattgt cagggtgagc ttggataccc tgatccggat tcctttttcc ggaggataat
     1561 atcaaatcat caatgaatgt caagccgtct atgttttgaa aaaaattagc aatttatgct
     1621 tgaaaaaata atatttttga ttagaatgaa ataaaaaata ccagagggaa gatgttcaac
     1681 ccctctccat gaggaggaag aaaatgtcga gagcaaaggt tggagacgtt ctgggttgtg
     1741 aagtatgcgg attgaaggtg attctggatg aagactgtgg ttgtgccaca atcgatctta
     1801 tctgctgtga agagccaatg gtcaatctcg gaccggcaaa acctaaaaaa gcacaggcca
     1861 ttgccgcacc cagaaaaaag acggtcgtga aaaaaaccaa agcagcgccc aagaaaaaac
     1921 cggcattgaa aaaagcaaag aaaccaaccg tgaaaaaagc tgttaaaaaa gcaacggtaa
     1981 agaaaacagc aaaaaaatct tcaaaaaaga aatagtgtcg tttcaggaag atagatatcg
     2041 actccgataa cactcgttgc tccgagggga ttgccggagt ttgttttttt agggattttt
     2101 cagcgcaacc cggatgttcg aagtaacgcc ccccctggtt ttcgtacgat ctgttccgga
     2161 cttgaaaaaa tacgcggatt ctgattaagc ttctctccga aggttccccc tatggcaaca
     2221 acaaaagata atcccagaaa aaaaagcgag gaactggatt cttttgctct aaaagtgaat
     2281 ctggaaagaa cggcggcaac catagaaatt ccggatcaat atgcgccgct tctcaaggtg
     2341 gtcgaaaacc attacggtct gcagaaaaaa atctacgaac ttttaacgga gctccatcat
     2401 ccttttgtca actgggaata cgtcttaaaa gaactgaaat cggtttccat cggcgatttc
     2461 tacatttata ataaacatca ggacggcctt tctgcactgg cgaccatgct ttgcatttac
     2521 tttgaggtca tcaaatcgtc atcggataag gacgtcaagg acaacgcggt ccattatctt
     2581 ttcgattata tcgatacgat actgaaccag agcaacgagc aaaccgccag aaatctgttg
     2641 ctgtttccgg aaattatcaa atctcttctg gatgcgatca atcagcagga aattattttc
     2701 aaaaaatgtt cgtcctatgc gaaaaaaatc atcaaaaatt ttccgggcca tggcgatgtg
     2761 ctgcaaaacc ggctgttcgt cgatttgctc tacaggattt tccggattac gtatcagttc
     2821 tggcttgaac aaccagatcc ctcccagtgg tttattgccg gggaaggcga atctccggaa
     2881 tcggtggctg cttacggaca attcatccgg ccactgagcc acgcgtccat gcggatgctg
     2941 atggagaaac tggaaacgtc ctatcaaaac acccatgaag ataaaaacga tgcgatcaaa
     3001 aattatctcg agatgccgga ttatttccag attgtgaacg gttatctgca aattgcggat
     3061 caactggaaa aatccaaaac ctacgagggt catcagcata tcaccaagct gcgatttctt
     3121 tttcatgtca tggaggttcc cagtcttgct gatattcacg ccagcgcctt gcgtgaaatc
     3181 aatcattcgc tcaaaatggt ttttcaggag gaacagcagg aaaaccttca tgattttgtg
     3241 cgcaagattt tcggtttttt gaagaaaagc aaatctcagc gtgaattcag cggcgccatt
     3301 tttgattgta ttacgataac ggcccgggaa gttttcgcgc aaaacagtca tgagcttgcg
     3361 aatacattta tcagcgagtt gattgcttac ggcttccagt atccggaaat caagggttca
     3421 aacgaagaat ggcaggttaa ggtgaatccg gcccatatca tgaatattcg tgcctggctg
     3481 gaaattatca gcatgaaacc ccgctggaca aaaaaattga tttcggcgct ggttattaat
     3541 ttgaaaatgg gcggtatttt tatccgcgat acggacctca ttcagaaaaa tatttccgct
     3601 ctgctcaaca cggatatcgc tcccgcgtac agtctgatca aacagcttct ccggatcttt
     3661 cccgtttatt tcagtgaaat cggtgcggaa ggtgagctcc gggacatcac gacaaaagtg
     3721 gacgaactgt ccatgcgcaa cgacaagctg gtgtatttct ttcggaaaca atcccacgtg
     3781 gaaagcaaca gcctgctcgt taaatttacc gaggatattt ttcagtactg gaataccgga
     3841 gatatgagtt atcttcgctc tcatcttccc gttgaagttt acgaacaggt gatgaatccc
     3901 ggagaatatt tcaccggaat gcacaaagcc tttaaatatt tcttcacaaa agttcaaagc
     3961 cccctgaaat ttctggactg ggataacgaa aaagtctgca aggaactggc gaacgtcaaa
     4021 ggcatcaatg cgagggatca ggaacgggtc aaaatcatga tccggatcta ccagctgatc
     4081 tacaaaaaat attacccaca gtttatcgat ctgctcaagg atctggaagc ggtcaacgct
     4141 tttcccaaag cggatatctc gtcgttgaaa cgctccttga cgacgaagaa ctatcatcgc
     4201 agcattacga ttattctgaa gtttctatcc attctcaagg aaagaattct ctcgcccaaa
     4261 aagacgcctt cttatgagaa catctattac aagaggcata ttgccgccgg cattccctcc
     4321 atgtacggaa catattacga aagaaaattc gaagcggtcg gcctttctct gcggctggaa
     4381 acactggcca ccatgctgtt cgaggaactg agcaaatcct tcaatctcaa gttcattacc
     4441 aagcacacga tcagaaaaat tcacacatac ctttggcttt acatgaatgc gctggaaatt
     4501 gaaggcatcg ccacggaagg tctggcagcc aaaatcaaat acgtcaccaa cgctttgtcc
     4561 atcaagcagt tttccctgga gcagtacgtg gatatttttc gtttcatttc caaggatatt
     4621 caggacatta ttcgcaacta ttacattgat atccacacca cctgcctgcc tgttgtgatc
     4681 ggccaggtat accggcggga aatcgattcc gagagaaaat ccggacaaaa gcaacatgag
     4741 aaactgatct atgcacagtc ggaaaatttc atccggggca tcatcgcatc cgctttcggc
     4801 attcaggtgc tggataattt cgttaattca gttattaaaa cactgaatgc ggaatccgaa
     4861 aagttcaggg acaacaaaca aattctgaag gtcgttatgg cgtacaatcc cgaactggcc
     4921 gtgtcttctt tctacaaaaa gaacaaaaaa gtagataatc agattctgct cgggaacaag
     4981 ggatatttcc tcaaagagct tgtctcattc gggtttaatg ttccgcccgg tttcatcatc
     5041 accacggaag tgttcagggg ctatgaagcg gtttacgggt ataaatatat ttttaacgat
     5101 ctggctgttc gggtcaacaa agaaataaaa aaactggaga agattacggg acggaagctt
     5161 ggcgaccgcc gcaatcctct gctgctctcg atcagaagcg gcgccacggt ttccctgccc
     5221 ggcatgatga aatctttttt gaacgtgggc atcaacgaga cgattgcgga aaatctcagc
     5281 gagaaagatg gttttaaatg ggccgcctgg gattcctatc gccggttttt acagatgtgg
     5341 ggcatgttta aagggctgaa tcgtaatttt ttcgacgcga tcattgacga attcaaacag
     5401 aagtacggcg tggaaagaaa aatccagttt tcacccgagc agatgaaaca gatcgccctg
     5461 tcctataaaa aagggctgac cgacaacggc atcgaattta aggagaagcc gctcgatcag
     5521 ctgcgcgacg ccattttgca ggttttcgat tcctggtatt cggaacaggc ggaaatttac
     5581 cgccggcaaa tgcatatttc cgataaatgg ggaacggcgg tcatcgtgca gaaaatggtt
     5641 ttcggtaatc tcaacgataa ttccggatcc ggcgttattt ttacccgaga accgaaaagc
     5701 acgtcttccg atgtgaccct ctacggagat ttcattttcg gggttcaggg ggacgatatc
     5761 gtttcgggac tggtggaaac gtatcccgtt tcggaaaagc agcgcatcgc ggaatgccgg
     5821 gattccaaga tttccctgga agataaattc cctgaaattt atgcggaact gctgagaatt
     5881 tcggagatcc tcatttacga aaaagggttc aatcaccagg agatcgaatt ta
//
LOCUS       DAOOLB010000111         5892 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_16200_length_5892_cov_41.520815, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000111 DAOOLB010000000
VERSION     DAOOLB010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5892
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16200_length_5892_cov_41.520815"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /gene="dusB"
                     /locus_tag="PLF77_10035"
     CDS             <1..867
                     /gene="dusB"
                     /locus_tag="PLF77_10035"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HPC09236.1"
                     /translation="YTEMISSNGLVRNTRKTYEYLQTCPEDRPLGAQIFGADPQVMAG
                     AARIVADTGVDLIDINMGCPVKKVIKTGAGAILMKDPDLAARIIKAVKKAVIIPVTVK
                     IRSGWNQSSINAVQIAKIAEDSGADAITIHGRTADQGYSGHADWKIIAEVKKAVKTLV
                     IGNGDIRQPQDAVQMLAETDCDAVMVGRAALGNPWIFKGISQALRGGEIECHPSLNQR
                     REMIENHWKMEARMLGVKLAGKSFRKHILWYTKGLDNSHRFREMAGKVNNEQEIFEAL
                     NNYFHLLNTEKI"
     gene            923..1198
                     /gene="zapB"
                     /locus_tag="PLF77_10040"
     CDS             923..1198
                     /gene="zapB"
                     /locus_tag="PLF77_10040"
                     /inference="COORDINATES: protein motif:HMM:NF017786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HPC09237.1"
                     /translation="MENHTLFNEGKVNSTVELEKFSKLEEKIKAIVEEKVSLKNQIKT
                     LEDTLKNKEAEIEKLNGKIKVLDEERNSVRARVDSLLDLIADVNSAK"
     gene            1217..1495
                     /locus_tag="PLF77_10045"
     CDS             1217..1495
                     /locus_tag="PLF77_10045"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPC09238.1"
                     /translation="MKNSYNITILGQELSVLSDSEDEQVAEVVQFLNKKVDEILHSGN
                     GLKTLNVAILAALNISEEFLKLKKVNKELCDKLENRAEELIQLIEETS"
     gene            1509..1706
                     /gene="ssrS"
                     /locus_tag="PLF77_10050"
     ncRNA           1509..1706
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PLF77_10050"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            1910..3478
                     /gene="rny"
                     /locus_tag="PLF77_10055"
     CDS             1910..3478
                     /gene="rny"
                     /locus_tag="PLF77_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPC09239.1"
                     /translation="MFDASFLSVLVLVVAVVSGIIIGFILKQSIAAKEIRSSKNLMAR
                     MAEEAKKEAETIKKEAVLQAKENLLKMKAEFDAESKERRNDFEAREKRIRTKEENLDK
                     KTDALAQKESSVESREKSISSKEQSLEDKRRKLDAALHEQQERLEKIAGISPEEAKNL
                     LIKSMEAEAKQDAAALVRKIEDDAKLTADKKAQEIISYAIQRYAGDVVAEKTVSAVSL
                     PSEEMKGRIIGREGRNIRAIEAATGIDLIIDDTPEAVVLSSFDPVRREVARISLERLI
                     QDGRIHPGRIEDIVKKVRTEVEQIVRETGEKASFDAGVHDIHPEIINLLGSLKYRTSY
                     SQNVLQHSIDVAYLTGIMASELKMNVREAKRAGLLHDIGKAIDHKVEGTHAAIGADYA
                     KRFGENPRIVQAIATHHDDGRNNTLLGVLVQAADTLSAARPGARREMLETYVKRLEEL
                     EKIANSFNGVDKCFAIQAGREIRILVENEKISENDATMLCKDIVKKIENELTYPGQIK
                     VTVIRETRVSDFAK"
     gene            3509..4297
                     /locus_tag="PLF77_10060"
     CDS             3509..4297
                     /locus_tag="PLF77_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HPC09240.1"
                     /translation="MKILFIGDIVGSPGRMAVRELLPDLKARKKIDFVIANCENGAAG
                     FGVTKKIMEELFRDDIDVLTSGNHIWNKREVLEFIGESDRLLRPANYPATAPGAGAVL
                     VPLKTGGYVGVLNLIGRIFMQAVDCPFITAKNKVAELREKTKVIIVDMHAEATSEKKA
                     LGWYLDGEVSAVLGTHTHVQTADEEILPNGTAYISDAGMTGPFDSVIGIRKEAIIERF
                     LTQMPNKFDLAKDDLRMQGVIMDIDVDSGKAKSIDRISVKLKDS"
     gene            4311..5594
                     /gene="tyrS"
                     /locus_tag="PLF77_10065"
     CDS             4311..5594
                     /gene="tyrS"
                     /locus_tag="PLF77_10065"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPC09241.1"
                     /translation="MKNAYDILKERGFVEQITDEGLIQELFAKGPVTCYIGFDPTATS
                     LHIGSLVPIMSLAHMQQSGNKPIALVGGGTGLIGDPSGKTEMRQVLTREQIDYNANCI
                     KQQLSKYLDFSDGKALLLNNADWLTKLNYIDFLRDVGRHFSVNRMLAAESYKIRMEKG
                     LNFIEFNYMVLQAYDFFHLFQNYGCALQMGGNDQWGNMLAGTELIRKIAAKDAHAVTF
                     PLITTSLGQKMGKTEKGTVWLDGALTSPYEYYQYWVNCDDADVERFLKLFTFLPLDEI
                     SAVKNLKDAQLNMAKTVLAFEATKVTHGAKAAEDAWRASAEAFHSRPVETGMFPSCNI
                     PRTAAASDTSAIPRYKIPRKDLKSGVQICGALAKAALTQSISETKRLIEQGGIYINDR
                     QVKSIDEKLTLADFGDAEEIRVRKGKKKYLIVEIE"
     gene            5646..>5892
                     /locus_tag="PLF77_10070"
     CDS             5646..>5892
                     /locus_tag="PLF77_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09242.1"
                     /translation="MQKKIERKKKPFHYRHANLRKKSKSNSHAAFYRPEIVPALKSTL
                     SRIGKPYPAPFVPDEFQVKALEAIKKTDCLVIAPTGSG"
BASE COUNT         1757 a         1248 c         1530 g         1357 t
ORIGIN      
        1 tatacggaga tgatcagttc caatggtctt gtccgaaaca ccagaaaaac gtacgagtat
       61 cttcaaacct gtccggagga ccgcccgctc ggagcgcaaa tttttggcgc tgatccacag
      121 gtcatggctg gggcagcgag gatcgtggcg gataccgggg tggatttaat tgatatcaac
      181 atgggctgtc cggtgaaaaa agtgattaaa accggtgccg gagctatctt gatgaaagat
      241 cccgatcttg ctgcccggat aattaaagcg gtaaaaaaag ccgttattat tccggttacg
      301 gtaaagattc gttcaggatg gaatcagtcg tctatcaatg ctgtgcaaat tgcaaagata
      361 gcggaggatt cgggagccga cgcaattacc atacatggcc gcacggctga ccagggttac
      421 agcggtcatg ccgattggaa aatcatcgcg gaagtcaaaa aagcggttaa aacactggtc
      481 attggtaatg gggacatccg tcaaccgcag gatgcagtcc aaatgcttgc tgaaactgac
      541 tgcgacgcgg tgatggtggg gagggctgca ttgggcaatc catggatttt caaaggaatt
      601 tctcaagcac tgcggggagg cgaaattgaa tgccatccct cgctgaatca acgtcgggaa
      661 atgattgaaa atcattggaa aatggaagcc cggatgctcg gtgtcaaact ggccggaaaa
      721 agttttcgta agcatattct ctggtatacc aaaggtttgg acaattcaca ccggttccgg
      781 gaaatggccg gcaaggttaa taacgaacaa gaaatctttg aggcactaaa taattatttt
      841 catttgctga atacggaaaa aatctaaaat aaggacttga cattaatgcg ataaattggc
      901 ataaaataag gaaaaagaaa ctatggaaaa tcatacttta tttaatgaag gaaaggtaaa
      961 ttctactgtg gaactcgaaa aattttcaaa gctcgaagag aaaattaaag ctattgttga
     1021 agagaaagtt tctttaaaaa atcaaattaa gacattggag gatacattaa aaaataaaga
     1081 ggcggaaata gaaaagttga acggaaagat aaaggtattg gatgaggaaa ggaacagcgt
     1141 acgcgcgagg gtagattcac tgcttgattt aattgcagat gtaaattcag caaaataagc
     1201 ttccagggcg tgtgttttga aaaacagtta caatataaca atattaggcc aggaactttc
     1261 tgttttgagc gattcggagg atgagcaggt ggccgaagtt gttcagtttt taaataaaaa
     1321 ggtggatgag atattacatt cgggcaacgg tttgaagaca ttgaacgttg ccatcctggc
     1381 agctttgaac atttcagagg agtttttaaa gttaaaaaaa gttaacaaag agctctgtga
     1441 caaattggaa aacagggccg aagaattaat tcagcttata gaggaaacaa gttagaaact
     1501 ttttttatta gcccctgcga tgtgcgtgat tgacatttgt ttttgagcca acattttgac
     1561 cttgggacct tatccttgat tgtggtgtgc atgtccgctt tttcctgagc tggtcgggaa
     1621 gcagaaagcc tgaaacaacc aataccggtt cccaccttgt taaaaggttc aaaaaaggtg
     1681 attaacacgg cataggcggg ggtctttttt atctgttcat caacatcaat tctttccagc
     1741 ctttagaaaa tcaataaaaa cgccaaacac cgaggtgaag catctgaacc attgatgcgc
     1801 gccgtggtta aatatagctc aaccggaaaa gaagtttata ttcttgaaat tgactgtgat
     1861 caggtcgggg aatgaggttt ccggaagggc accaaggagg taacatgata tgtttgatgc
     1921 cagtttttta tcagtgctgg ttttagttgt agcggttgtt tcagggatca ttattggctt
     1981 cattttgaag cagtccattg cggccaaaga aatcagatca tccaagaatt tgatggctcg
     2041 catggcggaa gaggccaaga aagaggctga aacaatcaag aaggaagcgg ttcttcaggc
     2101 caaggaaaat ctcctgaaga tgaaagccga atttgatgct gaatcgaagg aaagaagaaa
     2161 cgatttcgaa gcgcgtgaaa aacgcattcg caccaaggaa gaaaatctgg ataaaaaaac
     2221 cgatgctctg gcgcaaaagg agtcgtcggt tgaatccagg gaaaaatcaa tcagcagtaa
     2281 agagcaatcc cttgaagaca aacgtcgtaa actggatgca gcgcttcatg aacaacagga
     2341 gagactcgaa aagatagcgg gtatctctcc cgaggaagcg aaaaacctgc tgattaaatc
     2401 catggaagcg gaagccaaac aggatgccgc agcgcttgtc cgcaagattg aagacgatgc
     2461 caagctgacc gccgacaaaa aagcacagga aattatttcc tatgcgattc agcggtatgc
     2521 cggcgatgta gtggcggaaa aaacagtttc ggcggtcagt ctgcccagcg aagaaatgaa
     2581 agggcggatc atcggccgtg agggccgcaa tatccgcgcc attgaagcgg ccacgggaat
     2641 tgatttgatc attgacgata cgccggaagc ggttgttttg tcgagtttcg atccggtcag
     2701 acgcgaggtg gcgagaattt cgctagagcg cctgattcag gacggacgga ttcatcccgg
     2761 ccgtatcgaa gatatcgtca agaaagtcag gacagaggtc gagcagatcg tcagggaaac
     2821 gggtgaaaag gcatcctttg acgccggtgt gcacgacatc catccggaaa tcatcaatct
     2881 gctgggatcg ctgaagtacc gtaccagtta ttctcagaat gttttgcagc attctattga
     2941 tgttgcctat ttgaccggta tcatggcgtc ggaactgaaa atgaatgtcc gggaagccaa
     3001 gcgtgccggt cttttgcacg atatcggcaa ggcaatagac cataaagtcg aaggcacgca
     3061 tgcagccatc ggcgccgatt acgccaagcg tttcggtgaa aatccgcgta ttgttcaggc
     3121 gatagcaacg catcatgatg acggacgcaa caataccctg ctcggcgttc tcgtacaggc
     3181 ggcggatacg ctttctgctg ccagaccggg agcgcgtcgc gagatgctcg aaacatacgt
     3241 caaacgtctg gaagagctgg aaaaaattgc caattccttc aatggtgtcg ataaatgctt
     3301 tgccatccag gccggtcgtg aaatccgtat tcttgtggaa aatgaaaaaa tatccgaaaa
     3361 tgatgccacg atgctgtgta aggatatcgt caagaaaatt gaaaacgaac ttacttatcc
     3421 gggccagatc aaggtgaccg tcatcaggga aacaagagtt tctgattttg ccaagtaatt
     3481 tttccggtat gggaaaggaa cgatgcaaat gaaaatttta tttatcggtg acattgtggg
     3541 cagccccggc cgcatggcgg tcagggaact gttacccgac ctgaaggcca gaaaaaaaat
     3601 tgactttgtc atcgccaatt gtgaaaatgg agccgccgga ttcggggtga ccaagaaaat
     3661 catggaagaa ctcttcaggg atgacattga cgtgctgaca tccggcaatc atatctggaa
     3721 caaaagagaa gtcctggagt ttatcggaga atccgaccgg cttttacggc cggccaatta
     3781 tccggccacg gcgccgggcg cgggagcggt tcttgtccct ttgaaaacgg gcggctatgt
     3841 gggtgtcctc aacctgatcg gacggatctt tatgcaggcg gtcgattgtc cttttatcac
     3901 ggcgaaaaac aaagttgcgg aattgagaga aaaaacaaaa gtcattattg tagacatgca
     3961 tgcggaggca acctccgaga aaaaagcgct gggctggtat cttgacggcg aggtatccgc
     4021 agtcttgggg acccatacgc atgtgcagac cgctgacgag gaaattttgc cgaacggaac
     4081 ggcgtatatc agtgacgcgg gcatgacggg gccttttgat tccgtcatcg gtatccggaa
     4141 agaagctatt atcgagcgat ttttaaccca gatgccgaac aagttcgatt tggcaaaaga
     4201 cgatttaaga atgcagggcg tcattatgga tattgatgtt gacagcggca aggcaaaatc
     4261 cattgataga atcagcgtca aactgaagga ttcgtaagag gctggatgca atgaaaaatg
     4321 cttacgatat tttaaaagaa agaggtttcg tcgagcagat caccgatgaa ggcttgattc
     4381 aagaactttt tgcgaaaggt ccggtgacct gttacatcgg attcgatccc acggcgacca
     4441 gcctgcatat cgggtcgctg gtgcccatta tgtctctggc gcacatgcag caaagcggca
     4501 acaaacccat cgcgctggtt ggaggcggta ccggcctgat cggcgatccc agcggcaaaa
     4561 ccgaaatgcg tcaggttctc acccgcgaac agatcgacta caacgccaat tgcatcaaac
     4621 aacagctttc caaatacctc gacttttcgg acggtaaggc acttctttta aacaatgccg
     4681 actggctgac gaaattaaac tacatcgatt ttttgaggga tgtcggccgc catttcagcg
     4741 tcaaccggat gctggcggcg gaaagctata aaatcaggat ggaaaaaggc ctgaacttca
     4801 tcgaattcaa ttacatggtt cttcaggctt acgatttctt tcatctgttc cagaattacg
     4861 gatgtgcgct gcagatgggc ggcaacgacc agtggggcaa tatgctggcc ggcaccgagc
     4921 tgatccgcaa gattgccgcc aaagacgccc atgctgtgac gttcccgctc attaccacgt
     4981 ccctggggca gaagatgggg aagaccgaaa aaggcacggt ctggctggac ggcgctttaa
     5041 ccagtcctta tgaatattat cagtactggg tcaattgcga cgacgccgat gtggaacgat
     5101 ttttaaaact gttcacgttc ctgccgctgg acgaaatcag cgctgtgaaa aatttaaagg
     5161 atgctcaact gaacatggcc aagacggtgc tcgctttcga agcgacaaaa gttacgcacg
     5221 gagcaaaagc cgctgaagat gcgtggcgcg cgtccgcgga ggcatttcat tcccgtccgg
     5281 tggagaccgg tatgtttccg tcctgcaaca ttccccgcac ggcagccgcc agcgatacat
     5341 cggccatccc ccgctataag attccccgaa aggatttgaa atccggcgtc cagatatgcg
     5401 gagccttagc caaagctgcg ttgacgcaat ccatctccga aacgaaacgc ctgattgaac
     5461 agggcggtat ttacatcaat gaccgtcagg tgaaatccat tgatgaaaaa ctgacgcttg
     5521 ccgattttgg cgatgccgaa gaaattcgcg tgagaaaagg aaagaaaaag tatttaatcg
     5581 tagaaatcga gtaaaaaata tttccttgtg cggcacagtc ctcttttctc aaaagcataa
     5641 ctgaaatgca gaaaaaaatt gagagaaaaa agaagccctt tcattaccgg cacgcgaacc
     5701 tccgaaagaa atccaaaagc aacagccatg ctgcttttta caggcctgaa atcgttcccg
     5761 cgctgaaaag cactttatcc aggatcggca agccgtatcc agcccctttt gttcccgatg
     5821 aatttcaggt caaagctttg gaggcaatca aaaaaaccga ttgtctggtc atcgcgccca
     5881 cgggttcagg aa
//
LOCUS       DAOOLB010000112         5757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_16735_length_5757_cov_52.477902, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000112 DAOOLB010000000
VERSION     DAOOLB010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16735_length_5757_cov_52.477902"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1328
                     /locus_tag="PLF77_10075"
     CDS             <1..1328
                     /locus_tag="PLF77_10075"
                     /inference="COORDINATES: protein motif:HMM:NF018010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HPC09243.1"
                     /translation="KVAIRIDLVGNMKVKDAILNAGGLTANASMESGEIIRQYPNNEF
                     RTTYFNVARAMEGDPRDNLLLQDKDRIVIHSILEKNPRKNIFVTGDVTKPGTYQFTEN
                     MTVRDLIFKAGNVLESAYLDEAEITSVSVVNGRLGDLSHRKVNLKKALEGDAGHNVTL
                     LPNDRLHVKQIADYHNVRFITLSGQMAFPGKYPFRKGERLSDIVERAGGFTPHAYLRG
                     AQFTRVRVKELQQQGLKEMADRMERELLSGGAEQFATAVSKEEIAAKQGELEQKKKFV
                     DTLRKTRATGRMTVYVADAGKLRNTEYDFELEDGDTLYVPERNSVVNVVGSVMSQSSH
                     LYSENLSHQDYIDASGGYSYYADASNVYILKVDGGARRLSKNFIGWNSSRSRWEMTAY
                     GAGVKDIEPGDTIVVPEKIERIAWLREIKDITQILMNIAVVAGVTIKLF"
     gene            2005..2742
                     /locus_tag="PLF77_10080"
     CDS             2005..2742
                     /locus_tag="PLF77_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPC09244.1"
                     /translation="MVDRKFKKLSIVVPVFNEERYLEAVIGKVVAQPLPGGLEKELIL
                     INDASQDNSWNIMQALPEKFPSVTMQLINKTVNEGKGAALRDGFAKITGDIVIVQDAD
                     FEYDPADYPKLLQPILDDKADVVYGSRFIGEPHRVLYFWHQVANNILTTISNMLTNLN
                     LTDMEVCYKVFIREVADRLKLKSPRFGVEPEITAKIARMRLNGRHLRVYETGISYAGR
                     TYEEGKKINWKDAVSAIFQIIRFRFMD"
     gene            3023..>5757
                     /gene="ppdK"
                     /locus_tag="PLF77_10085"
     CDS             3023..>5757
                     /gene="ppdK"
                     /locus_tag="PLF77_10085"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPC09245.1"
                     /translation="MAKYVYSFSEGYGKSKNLLGGKGVGLAEMAHLKIPIPPGFTITT
                     EVCMEYLRNKGRYPQGLKEQVNKALDKMEKETGMRFGDPKNPLLCSARSGARSSMPGM
                     METVLNIGLTSKTIAGMIEKTNNPRFVLDSYRRLIQMYSDVVMEKAEGIEPEEGMGVR
                     QQLEREMAAMKERRNVKLDVDLTDKDLAQLAEIYKAKVKEVLGKPFPDDAMEQLWGAI
                     GAVFKSWNGKRAVSYRRIEGIPDDWGTAVNVQAMVFGNMGENSATGVAFTRNPATGEN
                     KFYGEWLPNAQGEDVVAGIRTPNPINEETRQSDDVDGHPSLEKAMPKAYTEIKAIRNK
                     LEAHFRDMQDLEFTIQDGRVWMLQTRTGKRNGPAAVRMAVEMYKSKMISAEEAISRVT
                     PSQLDEMLHPMVDPKAEATAEFLVKGLPAGPGGAVGQVAFTSDVAMEWRKEGKKVILV
                     REETNPEDVEGMRAAEGVLTARGGMTSHAALVARGWGKCCIVGAGTLHVDIAQKIMKV
                     DGKAVREGDFVTLNGTKGRAYIGSLPMISGAENDVNFAAFMKLCDSLRRLKIRTNAET
                     PKDAAKAVQFGAEGIGLFRTEHMFYGENSEQPLFYLRKMIIADTEEKRRAALDELFPF
                     VKGFIKKTIEAMGGRPVTIRLLDPPLHEFVPENPEERARLAESLGISVEKLNGRAEGL
                     HETNPMMGHRGVRLGISYPEVSEMQIRAILEGSAELIKEGKKALPEIMIPVVCDVAEF
                     SNQAALVKKVYPEVLAKYGLRKMSYMVGTMIEIPRAALLADKIAEEAEFFSFGTNDLT
                     QMTYGFSRDDVGAFVPGYLAKKIIPVDPFQILDQTGVGQLIQMGVERGRSARPGLKVG
                     ICGEHGGEPSSVMFCHRVGMDYVSCSPYRIPLARLAAAQAAVEEKMSSKPKTKAKA"
BASE COUNT         1488 a         1364 c         1637 g         1268 t
ORIGIN      
        1 tgaaagtggc aatccggatt gatctggtcg gcaacatgaa ggtgaaagac gccattttga
       61 atgccggcgg cctgaccgca aatgccagca tggaaagcgg cgaaattatc cgccagtatc
      121 cgaacaatga attcagaaca acgtatttca acgttgccag agcgatggaa ggcgatccga
      181 gagataatct tctgctgcag gataaagaca ggatcgtcat tcattcgatc ctggaaaaga
      241 atccaaggaa aaatattttt gtcacgggcg atgtcaccaa accgggaacg tatcagttta
      301 cggaaaacat gactgtgcgc gatctgattt tcaaggcagg caatgttctg gagtccgcgt
      361 atcttgatga agcggaaatt acatcggtca gcgttgtgaa cggaagactg ggcgatcttt
      421 cccacaggaa agtcaatctg aaaaaagcgc tggaaggcga cgccgggcac aatgtgacgc
      481 tgctgcccaa cgaccggctg catgtcaaac agattgccga ttatcacaat gtgcggttta
      541 tcacgttatc cggccagatg gctttccccg gcaagtatcc gttcagaaaa ggcgaaagat
      601 tatccgatat cgttgaacgg gccggcggtt ttacaccgca tgcgtatttg cgcggcgctc
      661 agttcacgcg ggtgcgggtg aaagagcttc agcagcaggg tctgaaagaa atggccgacc
      721 gcatggagcg tgaattatta tccggcgggg cggaacagtt tgccaccgct gtttcaaaag
      781 aggaaattgc cgccaaacag ggcgagttgg agcaaaagaa aaaatttgtc gacacgctgc
      841 gcaagaccag ggccaccggc cgcatgacgg tttatgtggc ggatgccgga aaactccgaa
      901 acacggagta tgattttgag ctggaagacg gcgacacgct gtatgttccg gaaagaaaca
      961 gcgtggtgaa tgtggtcggc tccgtgatgt cgcagagcag ccatctgtat tccgaaaatc
     1021 tcagccatca ggattacatc gatgcgtccg gcggatactc ctattatgcg gatgcgtcca
     1081 atgtctacat cctcaaagta gacggcggcg cccgacggct ttccaagaat ttcatcggtt
     1141 ggaattcttc ccgctcgcgc tgggaaatga cggcgtacgg cgccggcgtc aaggatatcg
     1201 aaccgggcga taccattgtt gttccggaaa agattgaaag gattgcctgg ctgcgggaaa
     1261 tcaaggatat tacgcagatc ctgatgaata ttgccgtagt cgccggtgtc acgatcaaac
     1321 tgttctaatg acaccgagtt gggacgttaa aattagggtt gtcacttcga gcgaatgcga
     1381 gaagtcttga aaaaggtttg agactttgca aaaccacgtc aattcgtcaa ttttgtcata
     1441 ccggcgcagg ccggtatcca gaaaatggat ccccgatagg accactcggg gatgacagag
     1501 gatggatcta accagagggc gccatggcct gtctcagcag gaatgaccca aagaaaaagt
     1561 tatgcaaggc tctcagtttg taactgtgaa atgaaagcat taagattcct cttcactggc
     1621 gctcatcgga atgacgcaga gttgggacgt taaaattagg gttgtcactt cgagcgaatg
     1681 cgagaagtct tgaaaaaggt ttgagacttt gcaaaaccac gtcaattcgt caattttgtc
     1741 ataccggcgc aggccggtat ccagaaaatg gatccccgat aggaccactc ggggatgaca
     1801 gaggatggat ctaaccagag ggcgccatgg cctgtctcag caggaatgac ccaaagaaaa
     1861 agttatgcaa ggctctcagt ttgtaactgt gaaatgaaag cattaagatt cctattcgct
     1921 tcggcttatc ggaagcgaca agtttctaca ggattgtaga tttttcgttt ctcgcaacca
     1981 gtgaatgcgg gggaaagtcg ccggatggtc gatcgtaaat ttaaaaaact ttcaatagtt
     2041 gttccggttt tcaacgaaga gcgttatctg gaagcggtca tcggcaaggt cgtcgcgcaa
     2101 ccgctgcccg gcggtctcga aaaagaattg attctgatca acgacgcctc gcaggataat
     2161 tcctggaata tcatgcaggc gctgccggag aaatttcctt ccgttacaat gcagttgata
     2221 aacaagacgg ttaatgaagg caagggcgcg gccctccggg acggctttgc taaaatcacc
     2281 ggggatatcg tcattgttca ggatgccgat ttcgagtatg atcccgccga ttatcccaaa
     2341 ttgttgcagc cgatcctgga tgacaaagcg gatgtggtgt acggcagccg cttcatcggc
     2401 gagcctcacc gcgtgttgta tttctggcat caggtggcca acaatatcct gacaactatt
     2461 tccaatatgc tgaccaatct taacctgacg gacatggagg tctgttacaa ggtttttatc
     2521 cgcgaggttg cggatcgtct gaaactgaaa agcccccgtt tcggcgttga gcctgaaatt
     2581 acggccaaaa tcgcccgtat gcgcttaaac ggcaggcatt tgcgggtata cgagaccggt
     2641 atttcctatg ccggccgcac ctacgaggaa gggaaaaaaa taaactggaa ggatgcggta
     2701 tcggccattt tccagattat ccgtttccgg ttcatggatt aggtattttt gacgccgagt
     2761 tatcacggcc gcttcccgta gccagcctct tggtccggga ggtttttctt ttccaaatca
     2821 tttcaataac atcaaatttt gtgttgtggc tgcttcattt tccaggccgg tcaatttcag
     2881 catgatatga atatgattga ttatttaaat agagcgattc gcgtcgattt tctacaattt
     2941 tatcttgaca atgagcacaa tcttatgacg atataacgca ttaaaattta ttggattaac
     3001 aaggaggttg agaagcaacg ttatggctaa atatgtatac tctttctcgg aaggatacgg
     3061 caaaagcaag aatcttttgg gcggcaaggg cgtcggcctt gcagagatgg ctcatcttaa
     3121 aattcctatc cccccgggtt ttacaattac aacagaagtt tgcatggaat atttgagaaa
     3181 taaaggccgt tatccgcagg gattgaaaga acaggtcaac aaagctctgg acaaaatgga
     3241 aaaagaaacg ggcatgagat tcggcgatcc gaaaaatcct ctcttgtgtt cagcccgttc
     3301 gggagcgcgc tccagcatgc ccggcatgat ggagactgtg ctcaatatcg gtctgacgtc
     3361 aaagacgatt gccggcatga tcgaaaaaac caacaatccc cgtttcgttc tggattcgta
     3421 ccgtcgtctg attcagatgt attcggatgt cgtgatggaa aaagccgaag gaatcgagcc
     3481 ggaagaggga atgggcgtcc gtcagcagct ggaaagggaa atggctgcga tgaaagaacg
     3541 ccggaatgtc aagctggatg tcgatttgac ggacaaagat ctggcccaac tggctgaaat
     3601 ttacaaggcc aaagtcaagg aagtgctggg caaaccgttt ccggatgatg ccatggaaca
     3661 gttgtggggc gcgatcggcg cggttttcaa gagctggaac ggcaagcgcg ccgtttctta
     3721 ccgccggata gaaggcattc ccgatgactg gggcacagca gtcaacgtgc aggcgatggt
     3781 tttcggcaac atgggcgaaa attccgccac gggcgttgcg tttacccgca atccggccac
     3841 cggtgaaaat aaattctacg gcgaatggct gcccaatgcg cagggtgaag atgttgttgc
     3901 gggaatccga accccaaatc cgatcaacga ggaaacccgc cagtcggatg atgtcgacgg
     3961 ccatccttct ctggaaaaag ccatgcccaa ggcttatacc gaaatcaagg cgatccgcaa
     4021 caaactggaa gcgcacttcc gtgatatgca ggacctggag ttcaccattc aggacggccg
     4081 cgtctggatg ctgcagaccc gtaccggaaa gcgcaacggt cccgccgcgg tgcgcatggc
     4141 cgtggaaatg tacaaaagca aaatgatcag cgcagaagaa gcgatttccc gtgttactcc
     4201 gtcacagctt gatgaaatgc tgcatccgat ggttgatccg aaagccgaag ccacagcgga
     4261 atttctggtc aagggtcttc cggccggtcc cggcggcgcc gtaggccagg ttgcgtttac
     4321 ctccgacgtg gcgatggaat ggaggaagga aggcaaaaaa gtcattctgg ttcgcgaaga
     4381 aacgaatccc gaagatgtgg aaggcatgcg cgcggctgaa ggtgtgctta cggcccgcgg
     4441 cggcatgacg tcgcatgcgg cgctggttgc ccgcggctgg ggcaagtgct gtatcgtcgg
     4501 cgccggcacg ctgcatgtgg acattgcgca aaagatcatg aaggtggacg gcaaggcggt
     4561 gcgcgaaggc gattttgtca cactcaatgg aaccaaggga cgcgcttata ttggctcgct
     4621 gccgatgatc agcggcgcgg aaaatgatgt caattttgcc gcttttatga agctgtgcga
     4681 ttcattgcgg cgtttgaaga tccgtaccaa tgcggaaacg ccgaaagacg ccgccaaggc
     4741 cgtacagttc ggcgccgaag gcatcggact gttccgcacg gaacacatgt tctacggcga
     4801 gaacagcgaa cagccgctgt tctatctgcg caaaatgatc attgcggaca cggaggaaaa
     4861 acgccgcgcg gcgctggatg aattgttccc gttcgtcaag ggcttcatta aaaagaccat
     4921 cgaagccatg ggcggaagac cggtcaccat ccgcctgctg gatccgcccc tgcatgaatt
     4981 tgtgccggag aatcctgagg aacgcgcgcg tctcgcggag agcctcggca tttcggttga
     5041 gaaactcaac ggccgcgccg aaggtctgca tgaaaccaat ccgatgatgg gccatcgcgg
     5101 cgtccgtttg ggcatcagtt accctgaagt cagcgaaatg cagattcgcg ccattctgga
     5161 aggctcggcg gagctgatca aggaaggcaa aaaagcgtta ccggaaatca tgattccggt
     5221 tgtctgcgat gtggccgaat tcagcaatca ggcggctctg gtgaaaaagg tttatccgga
     5281 agtgctggcc aaatacggcc tcaggaaaat gtcctacatg gtggggacga tgatcgagat
     5341 tccgcgcgcg gctctgctgg cggacaagat tgccgaagag gcggaattct tcagtttcgg
     5401 aacgaacgat ctgacgcaga tgacgtacgg cttcagccgc gacgatgtcg gcgcgttcgt
     5461 gcccggttat ctggcgaaga aaatcattcc cgttgatccg ttccagattc tggatcagac
     5521 cggcgtcggc cagttgattc agatgggcgt ggaaagagga cgcagcgcgc gtcccggtct
     5581 gaaggtcggg atttgcggcg agcacggcgg cgaaccgtca agtgtcatgt tctgccatcg
     5641 ggtgggcatg gattatgtca gctgttcgcc ttaccgcatt ccgctggcgc gtctggctgc
     5701 cgctcaggct gcggtcgaag agaaaatgag ctcgaagccc aaaaccaagg ccaaagc
//
LOCUS       DAOOLB010000113         5682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_17032_length_5682_cov_66.211125, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000113 DAOOLB010000000
VERSION     DAOOLB010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17032_length_5682_cov_66.211125"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLF77_10090"
     CDS             complement(<1..182)
                     /locus_tag="PLF77_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09246.1"
                     /translation="MLNINEIGVNAMIHARDHKTPYLIDPWDYLGPKRRKLLDGSWAR
                     VNLFSHKRSRGFRCYK"
     gene            443..1720
                     /locus_tag="PLF77_10095"
     CDS             443..1720
                     /locus_tag="PLF77_10095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HPC09247.1"
                     /translation="MNVAAKTYQKGEIPGVIAIKASVCKGCDACKRHCPTGAITGMFG
                     ATHSINADLCLSCGQCLINCPFGAPYETSDTVDAVIAKLQDKNTTVVGIIAPAVRVAI
                     GEDFGLPAGSLVTGKLYGSMRKAGFDILDNNFTADLTIMEEGSEFIARARHAVFGEEN
                     AHGGHIGPLPQFTSCCPAWVRFVETKYPALIPHLSSAKSPQQMAGTVAKTYGVKVWNK
                     PADRIYTVGIMPCTAKKLEASRPEFISAYEYNSKKGQAAGASYPDVDTVLTTRDLSRL
                     FKKLGINLAAEKEMEGGTLLADYTGAGTIFGNTGGVMEAALRTAYAVITGNELKPLEF
                     KKVRGLNGVKSASVTIRDSKYSKDVTINVAVVHGIARNIAPVVDEVLAGRSPYHFIEV
                     MNCPGGCINGGGQPIYGNGSSWIDAIRPWFAWR"
     gene            1739..2059
                     /locus_tag="PLF77_10100"
     CDS             1739..2059
                     /locus_tag="PLF77_10100"
                     /inference="COORDINATES: protein motif:HMM:NF014327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HPC09248.1"
                     /translation="MEEKSYQYMENPLHVTRREFITIGGIVIAFLALPAVWFKSIATS
                     NNQYIQARTKGLYQDDEKSAVRVSHANQSVMRYYKEFGGEPLGHLSHELLHTGYINRS
                     KGLI"
     gene            2071..2733
                     /locus_tag="PLF77_10105"
     CDS             2071..2733
                     /locus_tag="PLF77_10105"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HPC09249.1"
                     /translation="MMHHEKMILKHPWRVRMFHYLLIVSFLPLAVTGVILYFKPLAEE
                     SMNFAMQIHVAAGVVLTLDAAAFFLLAFDRVILFIERIFTFSMDDVRWFAILGGYPQK
                     MLLHKKVAVPPMGKYNSGQKLFGICVFIGGTVLILSGLVLWAFPHLTPRAAVAYLGTL
                     HTFFAWVLTLFLCVHLFLGVYMFDDFKAMILHGKISYEEAKEMAPRWVEKEIVHIAEK
                     QS"
     gene            2808..4205
                     /gene="hydG"
                     /locus_tag="PLF77_10110"
     CDS             2808..4205
                     /gene="hydG"
                     /locus_tag="PLF77_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPC09250.1"
                     /translation="MIKDFIDDDRIQNILNQTINPDPLHVREVIDKAYELKGLTPEEV
                     AVLLQTEDENLIGAILQAAHKIKQDIYGNRLVLFAPLYVANHCSNNCLYCGFRRDNKE
                     LNRVALNLDQIRKEVEVLEHEGHKRLLMLCGEHPSRSSLDYFIESIEAAYATKTENGG
                     EIRRINVELAPLEVDEFRRLKKTGIGTYLLFQETYHHETYKKMHPSGPKKDYVNRLTA
                     MHRAQEAGIDDVGLGALFGLYNYKFDLLGLLFHALQLEKDCGVGPHTISIPRLEPAFN
                     APAAIQPPMPVSDRDFKKLVAILRMAVPYTGMILSTREKPELRSEVFNYGVSQISAGS
                     RTNPGGYSDTTSDRYRAAQFNLGDTRTLDEVILDITERGHISSFCTACYRLGRTGKDF
                     MDLAKPGLIQKFCQTNAVFSFKEYLLDYASPKTKKAGEKLIAEMLEKSFKSQRKKMLI
                     NRLQTIEEGKRDVYI"
     gene            4192..5253
                     /gene="hydE"
                     /locus_tag="PLF77_10115"
     CDS             4192..5253
                     /gene="hydE"
                     /locus_tag="PLF77_10115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPC09251.1"
                     /translation="MSISDHIDMLLLKAEIGKPLSREEMVELLKMPGEYSVDLFAAAD
                     RVRENNVGNEIYLRGIIEFSNFCERNCLYCGLRKTNEKLERYRMSGDEILETAREIKA
                     TGIATVVLQSGEDSHYTAERICKLVQAVKEETDLVITLALGERSPEDYRAFFDAGADR
                     YLLKHETASPELYKYLRPGCRLDARVSCLKMLKNLGFEAGTGNMVGLPGQTLEILADD
                     LLLMKLLDADMLGIGSFIAHPDTPLAGIDNDDIELALRVIAIARLLTRNTNIPATTAL
                     ATLNPHGRLQALQAGANVVMPDFTPEIYRVRYEIYPGRKNDLQSADSIAQLEKDLHRI
                     GRTMNYGTGQRNKHRPIKS"
     gene            5270..>5682
                     /locus_tag="PLF77_10120"
     CDS             5270..>5682
                     /locus_tag="PLF77_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPC09252.1"
                     /translation="MVTVKLTIDNRELEAPVGATILEAARIAGIKIPTLCASPEIKHT
                     PGACRVCMTEVEGQRSLIAACVFPVFEGMVVHTNTEKVRKARKMVVELLLANHPQECS
                     HCVRNGNCELQKVAEFVGLKEIRFPFTEFPQKENF"
BASE COUNT         1478 a         1454 c         1462 g         1288 t
ORIGIN      
        1 tatttgtaac aacggaatcc tcttgatctt ttatgagaga ataaattcac cctggcccag
       61 gatccgtcta aaagtttgcg tcttttgggt cccaggtagt cccatgggtc gattaagtag
      121 ggcgttttgt gatcacgggc atgaatcatg gcgttaacac ctatctcatt aatatttaac
      181 atgattcata ccatttcaga gaaaaaccgt caagggaaaa atgcacaaat actaaattat
      241 tttaaagcgt tataccaatt ctgacttttt acgagggcgt caaaattagt tttgtccatt
      301 tttttaacat tctttcttta agaacttgac aaagtttgac gaattcttta tatgcttatt
      361 ttggcatatg ccagcattga catatgcatc catgcacata aggaccgttt caagcgtcaa
      421 aacataacgg aggaaaagga aaatgaacgt agctgcaaaa acatatcaaa aaggggaaat
      481 tcccggcgtg atcgccatca aagcgtccgt ttgcaaggga tgcgacgcct gcaagcgaca
      541 ttgtccgacg ggcgccatta cgggcatgtt cggcgcgacg cacagcatca atgcggattt
      601 gtgcctgagt tgcggacaat gtctgatcaa ctgtccgttc ggcgcgcctt atgaaacgtc
      661 ggatacggtg gacgcggtta tcgccaaatt gcaggataag aataccacgg tcgtcggcat
      721 catcgctccg gccgtgcgcg tggccatcgg cgaggatttt gggcttcccg cgggaagtct
      781 cgtgaccgga aaactctacg gctcgatgag aaaagcggga tttgacattc tggacaacaa
      841 tttcacggcg gatctgacca tcatggaaga agggtcggaa ttcatcgccc gcgcgcgcca
      901 tgcggttttc ggagaggaaa acgcgcacgg cgggcatatc ggcccgttgc cgcaatttac
      961 ctcctgctgc ccggcctggg tccggtttgt cgaaacgaaa tatccggcgt tgatcccgca
     1021 tttgtcatcg gccaaatctc cgcagcagat ggccggaacc gtcgccaaga catacggagt
     1081 caaagtatgg aacaaaccgg ctgacaggat ttatacggtg gggatcatgc cctgcacggc
     1141 caagaaactg gaggcctcgc gtcccgaatt catcagcgcc tatgaataca acagcaaaaa
     1201 aggccaggcc gccggcgcgt cctatcccga tgtggatacg gtgctgacaa cgcgggacct
     1261 gtcgcgactt ttcaaaaagc tcggcatcaa tctggcagcc gaaaaagaga tggaaggcgg
     1321 gacgctgctg gccgattata ccggcgcggg cacgattttc ggcaacacgg gcggcgtcat
     1381 ggaagcggcg ctgcgcacgg cgtacgcggt gattaccggc aacgagctga aaccgctgga
     1441 attcaaaaaa gtccgggggc tcaacggcgt gaaaagcgcg tcggtgacca tccgcgacag
     1501 taaatacagc aaggacgtta cgatcaacgt cgccgttgtt cacggaatcg cgcgcaatat
     1561 tgcgcccgtc gtggatgaag tcctggcggg acgttcgccc tatcatttta ttgaagtcat
     1621 gaactgtccc ggcggatgca tcaacggcgg cggccagccg atatacggaa acgggtcgtc
     1681 gtggattgac gccatccgtc cctggtttgc atggagatag gaaaaaagga gaaatcaaat
     1741 ggaagaaaaa tcatatcaat acatggaaaa tccgctgcat gtcacccgcc gcgaattcat
     1801 taccatcggc ggcattgtta tcgcttttct ggcgctgccc gccgtctggt tcaaaagcat
     1861 tgcaacgtcc aacaaccagt atatccaggc gcgcacgaaa gggctgtatc aggatgatga
     1921 aaaatcggcc gtgcgcgtca gccacgccaa ccagtccgtc atgcgctact ataaggaatt
     1981 cggcggcgag ccgctgggcc atctttccca cgaactgctg cataccggct acatcaaccg
     2041 atccaaaggt ctcatttaga aggaggatag atgatgcatc acgaaaaaat gattttaaaa
     2101 cacccttggc gggtgagaat gtttcattat ctgctgatcg taagtttcct gccgctggcc
     2161 gtcaccggtg tcattttgta cttcaagccc ctggctgaag agagtatgaa ttttgcgatg
     2221 cagatccatg tcgccgccgg cgtcgtcctg acgctggacg cggcggcttt ttttctgctg
     2281 gcattcgacc gggtgattct tttcatagaa cgaatcttca cgttttccat ggacgatgtg
     2341 cgatggttcg ccatactggg cggctatccg caaaagatgc tccttcataa aaaagtcgcc
     2401 gttcccccca tgggaaaata caattccgga cagaaactgt tcggcatctg tgttttcatc
     2461 ggcggcacgg ttctgatcct cagcggattg gttttgtggg ctttcccgca cctgaccccg
     2521 cgggccgccg tggcctatct gggaacgctt catacatttt ttgcctgggt gttgacgctg
     2581 tttctttgcg tccatctgtt tctgggcgtc tatatgttcg atgatttcaa ggcgatgatc
     2641 ctccacggga aaatttccta cgaggaggcc aaggaaatgg cgccgcgctg ggtggaaaag
     2701 gagattgttc acattgctga aaaacagagt taacaaaatg atccttatga aaatcatgtt
     2761 gccatcaaca aggctctgcc ggaaaagact aaaatatcag atttaaaatg ataaaagatt
     2821 ttattgatga cgatcgtatt cagaatattc tgaatcaaac gataaatcct gacccgttgc
     2881 acgtccgcga ggtgatcgac aaagcatacg aactaaaagg tcttacaccc gaagaagtcg
     2941 ctgtgctgct gcagacggaa gatgaaaacc tgatcggcgc gatccttcag gcggcgcata
     3001 aaatcaagca ggatatatac ggcaaccgtc tggttttatt cgcgccgctt tatgtggcca
     3061 atcactgttc caacaactgt ctatactgcg gcttcaggcg ggacaataag gaactcaatc
     3121 gtgtagcgct aaacctggat cagatccgga aagaagtgga ggtcctggag cacgaaggcc
     3181 acaaacgcct tttaatgctc tgtggcgagc atccgtcgcg ttcgagcctg gattacttca
     3241 ttgaatccat cgaagcggcc tatgccacca aaacggaaaa tggtggagaa atccgccgca
     3301 tcaatgtgga acttgctccg ctcgaggttg acgaattccg ccgtctgaaa aaaaccggca
     3361 tcggcaccta cctgcttttt caggaaacgt atcatcatga aacctacaaa aagatgcatc
     3421 cgtccggccc caaaaaagat tatgtaaacc ggttgaccgc catgcaccgt gcccaggaag
     3481 ccggcatcga cgacgtgggc ctgggcgcgc ttttcggact ctacaattat aaattcgatt
     3541 tgctgggact gttgttccac gccctgcaac tggaaaaaga ctgcggcgtc ggtccgcata
     3601 cgatttccat cccccggctg gaacccgcat tcaacgcgcc ggcggccatc cagccgccca
     3661 tgcctgtttc cgatcgcgac ttcaagaagc tggtcgccat tttgcgcatg gccgtaccct
     3721 atacaggaat gattctgagc acacgggaaa aacctgagct gcgttcagaa gtttttaact
     3781 acggcgtttc ccagatcagc gcaggctccc gcaccaatcc cggtggatat tcggatacga
     3841 cgagcgaccg ctaccgcgcc gctcaattca acctggggga cacccgcaca ctcgatgaag
     3901 tcattctcga tattaccgaa cgcggccata tctccagttt ctgtacggcc tgttaccgtc
     3961 tgggccgcac gggcaaagac ttcatggatc tggccaaacc tggtctcatt cagaaattct
     4021 gtcagaccaa tgcggtgttt tctttcaagg agtatctgct ggactatgcg agcccgaaaa
     4081 ccaaaaaagc aggtgaaaaa ctgattgccg aaatgctgga gaaatcgttt aagtcccagc
     4141 gcaaaaaaat gctcatcaac cgtctgcaaa ccatcgaaga agggaagcgg gatgtctata
     4201 tctgaccaca ttgacatgct gctattgaaa gcggagatcg gcaaaccgtt aagcagagag
     4261 gaaatggttg aacttttaaa aatgccgggt gagtattccg tggatctttt cgctgcggcc
     4321 gaccgtgtca gagaaaacaa tgtcggcaat gagatttatc tgaggggaat tatcgaattt
     4381 tccaatttct gtgaacgaaa ctgcctttac tgcggtctgc gcaaaaccaa tgaaaaactg
     4441 gagcgctacc ggatgtccgg agatgaaatt ctggagacag cgcgcgagat caaagccacc
     4501 ggcattgcca cagttgtgct gcaatccggg gaagactccc attacacggc cgaacgcatc
     4561 tgcaaactgg tgcaggcagt caaggaagag acagacctgg tgataacgct ggctcttggg
     4621 gaaagatcgc ccgaagatta ccgggcgttc ttcgatgctg gcgcagaccg ttatctgctc
     4681 aaacacgaaa ccgcctcgcc ggaactgtat aaatatctgc gtccgggctg ccgactggat
     4741 gcacgcgtct catgcctgaa aatgctcaaa aatctgggat ttgaagcggg gacgggaaac
     4801 atggtgggac tgccgggaca gactcttgaa attttggctg atgatctttt gctcatgaaa
     4861 ctgctcgatg ccgacatgct gggtatcggg tcgttcatcg cccatccgga cacaccgctg
     4921 gccggtattg ataacgacga tattgaactc gctttgcgcg tcatcgccat tgcccgtctt
     4981 ttgacccgta acaccaatat cccggcaact acggcgttgg ccacgctcaa tccccacggg
     5041 cgtcttcagg cccttcaggc gggagcaaac gttgtgatgc cggattttac gccggaaatc
     5101 taccgtgtcc ggtatgaaat atatcccggc agaaaaaatg atctacaatc ggcggacagc
     5161 atcgcccagc tggagaaaga tctccatcgc ataggaagaa caatgaatta tggcaccgga
     5221 caaagaaaca aacacagacc aatcaaatca tgaaaaggag caatcattta tggtaaccgt
     5281 taaacttact attgataatc gtgagctaga agcgcctgtt ggtgcaacca ttcttgaggc
     5341 tgcccgcata gccggcatta agatccccac gctttgcgcc tcgccggaaa tcaaacacac
     5401 ccccggcgcc tgccgcgttt gcatgacgga agtggaaggt cagcggtcgc tgattgccgc
     5461 ctgcgttttc ccagtcttcg aaggcatggt tgtccacacc aacacggaaa aagtccgcaa
     5521 ggcgagaaaa atggttgtgg aactcttact ggccaaccat ccgcaggaat gcagccattg
     5581 cgtgcgcaac ggcaactgtg aactgcagaa agtggccgag tttgttggcc tcaaagaaat
     5641 tcggttcccc tttaccgaat ttccgcaaaa agaaaatttt at
//
LOCUS       DAOOLB010000114         5655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_17143_length_5655_cov_48.852857, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000114 DAOOLB010000000
VERSION     DAOOLB010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17143_length_5655_cov_48.852857"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2652
                     /locus_tag="PLF77_10125"
     CDS             <1..2652
                     /locus_tag="PLF77_10125"
                     /inference="COORDINATES: protein motif:HMM:NF021504.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HPC09253.1"
                     /translation="AWTLAAVTGREVIVPHNPGAMGAWGIGLCAIRELGEEKLFAAGT
                     FDLNLALNARVVSRIEFRCADSKCHNFCHIDKTTVSVGAAEKTVLSGGACPKYEISSI
                     ASKKIPKEAPNALEERMALLENFREAGTGDREVGVPYVGVLNGYLPWMVTFLSEIGFR
                     VKVLRSDSKSLAGGEERCFSFDSCAPAKIAHAVADADVDTLVFPKFLNLHDRDGHGGR
                     TCPMEQGMADMADQALKARGRNIRFVKPLLNLRDGLLNRRLIGRLRGLARELGVDDRH
                     VKTAARRAAKAQQDYEHALAEIGRRTLAYGRGQGIPVVAVIGSLHVIYDRAVNASIPD
                     LLRQNGVLSLPMDCYPIPDDIPELPRIVWEDANRALRVALSARNCGEVFPLFLSSFGC
                     GPASFIEHFFRFLMEGYPHTALESDGHGGFAGFVTRIQAFLHTVRQHDGRPVPAESSR
                     LRLFDTLERPPLASQKDSKFVVFTMGDQTAPIMAAVYRSWGYDAIPAIPTSAVSLAAG
                     RRDCSGKECLAYQLIWGSFRKYLDDNPPEKRTVLLQISGRGACRTCNFSMKDQISIEN
                     MGLSDRVSLRHVAAELECPGLVSRFYTGTLVWNLLNQLAAYHRPYESEPGEVDRIYDD
                     YCSDLIRILEKPSGRGFSGIISKMGSVGRIVSGSIEQNQGSALFEQLVRRASTQFAAM
                     RKCRTDQNSLRTIFLTGDIYLRLDDFGSDAIIRRLNARGIRVIVEPLWVLAEYLVEER
                     SAELFSLPQGTFMNAMAKVLLPKSRKHFYEMAQEQNPWLPTFDVPEMLKKAKPIIDRH
                     PRGEAPITVGSVIHHWDKAVSDGIVIVGPWGCGPALIAESLLKHQRQIPMLFVYNDGS
                     PLDERKLNGFVFRLRRQEPKTATETGR"
     gene            complement(2982..3500)
                     /locus_tag="PLF77_10130"
     CDS             complement(2982..3500)
                     /locus_tag="PLF77_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09254.1"
                     /translation="MTRSDMKKNKSKTKIILAVLIVGIAVIAGIVYWHSGNDGDTGAA
                     VDATQPVALNMSEQDIVDAEKNASIAAVSGETVAGTVTERPDYITEIEWQVLQSVTRQ
                     NPDIKLNDLVNKMLFAKKKQAWLSAEKDSAERKLLAKQLLDMIPGQVAMEAIDGATAQ
                     TMEAELHADLLR"
     gene            complement(3627..5333)
                     /locus_tag="PLF77_10135"
     CDS             complement(3627..5333)
                     /locus_tag="PLF77_10135"
                     /inference="COORDINATES: protein motif:HMM:NF015541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004806 - triglyceride lipase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HPC09255.1"
                     /translation="MAFYEKPSTIPGTHGTLIRYRTSTSVPPGGPSCYSWTVMYASQD
                     ALGEPNVVTGTVLVPKSLYNYGLTARPIISYAVGTHGLVQSWAPSIQLENGTDYENSN
                     IAAALNRGYAVLITDNPGYTNNDVPSYMVGKAQGQAVLDIVTAALQIPNISLKSYAKV
                     GIWGYSQGGQSAAWAGQLQSTYAPGIKLAGVAAGGVPAELFSVGRYLDGNNGSSFLLS
                     TCIGLNAQYPNDILLNSRVNTDGMDAIGRGLSMGVFEALFAFMNTPLSDFTKYGETSD
                     QIFAIPEIAEVVNGQELGKTKINAPVFLYHGTGDEFIPLEQALNLKEKYCSLGVNTSY
                     MVYPGEHITTQFQAAPQVLDWLKDRFAGKSAASTCRTSNPRPASTANPVDGDFLFSLD
                     GWKLDGMIKLKTLMTKVSLPEGSTFSAETNMTNNTITGGMDIPEFSYYIYAFGLMPLQ
                     VKLKIVPAGTMTGTASLDKNGILHINGNVKADIYLKKVGELGIGIPFSLKTKTPVDFP
                     IVFDGPVSSLGDGSLTFTGTTTFPDMVENGIIINALFTGLMSGPGQEFTFTVTPPAPV
                     AW"
BASE COUNT         1359 a         1451 c         1450 g         1395 t
ORIGIN      
        1 gcctggactc tggccgcggt cacgggtcgg gaggtaatcg ttccgcataa tcccggggcc
       61 atgggcgctt ggggcatcgg tctgtgcgca atccgggagc tgggcgaaga aaaacttttc
      121 gcagccggca ctttcgatct gaatctggcg ctcaacgcca gagtggtttc cagaatagaa
      181 ttccggtgtg ccgattccaa atgtcataat ttttgtcata ttgataaaac gactgtctct
      241 gtcggcgcag cagaaaagac cgttctgtcc ggaggcgcct gcccgaaata cgaaatctca
      301 tcaatcgctt caaagaaaat tcccaaggag gccccgaacg cgcttgaaga aagaatggcg
      361 ctgctggaaa atttccggga agccgggaca ggcgatcggg aggtgggggt gccttatgtc
      421 ggagtgctga acggatacct gccctggatg gtgacttttc tgtcggaaat cggattccgt
      481 gtaaaggtgt tacgctctga cagcaaatcg ctggcgggcg gtgaagaacg ctgtttttcg
      541 ttcgattcct gcgctcctgc taaaatcgcc cacgcggtgg cggatgcgga tgttgacacg
      601 cttgtattcc cgaaatttct gaatctgcat gacagggacg gccatggcgg ccgcacgtgt
      661 ccgatggagc agggcatggc ggacatggcc gaccaggcgc tgaaagctcg cggccggaat
      721 atcaggttcg tcaagcccct tctcaacctg agagacggcc tgctgaaccg gcgactcatc
      781 ggccgccttc gagggctcgc ccgggaactg ggcgtcgatg atcgacatgt caaaacagcg
      841 gcccgccgcg cggcaaaagc gcagcaggac tatgaacatg cgctggcgga aatcgggcgg
      901 cgaaccctgg cctacggccg gggacaggga attccggtgg ttgccgtcat cggttcgctg
      961 catgtgatct atgatcgcgc ggtcaatgcc agtattccag atcttctcag gcaaaacggc
     1021 gtactgtcgc tgcccatgga ctgttatccg attccggacg atatccctga acttccccgc
     1081 atcgtttggg aagatgcgaa cagggctctc cgtgtcgcgc tttcagccag gaattgcgga
     1141 gaggtgttcc ccctttttct ttcctctttc ggctgcggac cggcctcgtt tattgaacat
     1201 ttctttcggt tcctgatgga aggttatccg catacggctc tggaaagcga cgggcacgga
     1261 ggctttgcgg gtttcgtgac aagaatccag gctttcctgc acaccgttcg tcagcatgac
     1321 ggccggccgg ttcccgcaga atcgtcaaga ctccgcctgt tcgacacact ggaacgcccg
     1381 ccgcttgcat cgcaaaagga ttcaaaattt gtcgtcttta ccatgggcga ccagacggct
     1441 ccgattatgg cggctgtcta ccggtcgtgg ggctatgacg cgatcccggc aattcccaca
     1501 tcagcagtgt ctctggctgc ggggagaaga gactgttcag gcaaagagtg ccttgcctac
     1561 cagttgatct ggggttcgtt tcggaaatat ctcgatgaca acccgcccga gaaacgcacg
     1621 gtcctgcttc aaatcagcgg aaggggcgcc tgccgcacct gcaatttctc catgaaagat
     1681 cagatatcga tcgagaatat gggcctttca gatcgtgtgt cgctgcgcca tgttgccgcc
     1741 gagctggaat gccccgggtt ggtcagccgg ttttacacgg gaacgcttgt atggaatctt
     1801 ctgaaccagc ttgctgccta tcaccgccct tatgaatccg aaccgggaga agtcgacagg
     1861 atatacgatg actattgcag cgaccttatc cggatactgg aaaaaccttc cggaagaggt
     1921 ttctccggaa tcatttcaaa gatgggatct gtcggacgga tcgtctccgg aagtattgag
     1981 cagaaccaag gcagcgctct tttcgagcaa cttgttcgcc gcgcatcgac acagtttgca
     2041 gccatgcgga agtgtcggac ggatcaaaat tctctccgga cgattttcct tacgggagat
     2101 atctatctgc gtctggatga tttcggaagc gacgccatta tccgacggct gaatgctcgc
     2161 gggatacggg tcatcgtgga acctttatgg gtactggccg aatatctggt tgaagaacgt
     2221 tccgccgaat tattcagtct gcctcaggga actttcatga atgccatggc gaaggtcctc
     2281 ctgccaaaat cgcgaaaaca cttttacgaa atggctcagg aacaaaaccc ctggctgccc
     2341 acattcgatg ttccggaaat gctgaaaaaa gcaaaaccga tcatcgaccg gcacccgcgt
     2401 ggcgaggccc cgataaccgt gggaagcgtt attcatcact gggataaggc ggtctcagac
     2461 ggtatcgtga ttgttggacc atgggggtgc ggtccggcac tgattgccga aagcctgctc
     2521 aaacatcagc ggcagattcc gatgctgttc gtttataacg acgggtctcc tctcgacgaa
     2581 cgaaagctta atggattcgt attccggtta cgcaggcaag aacccaaaac ggcaacagaa
     2641 accggccggt aagaaaatct ccgcctcatg agagtctttc ttgatgcctg tgtgaaaaaa
     2701 atcaacgggg ctatcccggt cggacagcct ctttcatcgt ttattcaatc cgtgtaagtt
     2761 tagaatcccc ttgccttaaa atttttggca accttaccgt aaaattcttt tgcatccccg
     2821 tcaggggcaa taccgaaaac gcaattcatc tcacagaaat gattgatgcc cggatacaac
     2881 aaagacgtgc tcatgatgcc gaggagaaca acctgaccga tttccctttt ccaacaaact
     2941 ctaaagtgtc cccggatttc ggatttggat ttgcggatct tttatcgcaa caagtccgca
     3001 tgaagttccg cttccattgt ttgggctgtt gcaccatcaa tggcctccat agcaacttga
     3061 cccggaatca tatccagcaa ttgcttggcc agaagtttgc gctcggctga atctttttcg
     3121 gcagaaagcc atgcctgctt ttttttggca aacaacattt tgttgaccaa atcgtttaat
     3181 ttgatatccg gattttgcct ggttacgctt tgaagcacct gccactcgat ttcggttata
     3241 taatcaggtc tttctgttac cgtaccggca accgtttctc ccgaaacagc agctatggac
     3301 gcatttttct cagcgtccac gatgtcttgt tcagacatgt ttaatgcgac gggttgtgtt
     3361 gcatcaacag cggccccggt atcgccgtca ttgccgctat gccaatacac aatgcccgca
     3421 atcactgcaa ttccgactat cagcacagcg agtattattt ttgtcttgga cttatttttt
     3481 ttcatatcgc ttcgggtcat ttatagactc tccttgtgtc attgccttgt tacgatatta
     3541 aaatatcata tcgtgaaaat atatctccgg ccaggccatt gtcaggcagc ctgaccggag
     3601 atgtgaaaaa aatcagataa aaaatcttac cacgctaccg gcgcaggcgg cgtcacggtg
     3661 aaggtaaatt cctgaccggg accggacatc aaacccgtaa acagggcatt gataataatc
     3721 ccattttcga ccatgtccgg gaatgtcgtg gttcccgtga atgtcagaga accatctccc
     3781 agcgatgata caggtccgtc gaagacgatt gggaagtcaa caggcgtctt ggttttcagc
     3841 gagaagggaa tcccgattcc cagttcaccg acctttttca gataaatgtc tgctttgaca
     3901 tttccgttga tatgcaggat tccgttttta tcgagtgagg ctgtgcctgt catggttcct
     3961 gcgggcacga tcttcaattt cacctgcaac ggcatcaaac caaacgcgta gatgtagtaa
     4021 ctgaattccg ggatgtccat accgcccgta atcgtattat tcgtcatatt cgtttccgcc
     4081 gagaacgttg atccttccgg taaagatact ttcgtcatga gtgttttcag tttgatcatg
     4141 ccgtcaagtt tccaaccatc gagagaaaaa agaaagtcgc cgtcaacggg attggctgtg
     4201 gatgccgggc gcgggttgga agttctgcag gtactggctg cgcttttccc ggcaaaccgg
     4261 tccttcaacc agtcaagcac ctgtggtgcg gcctgaaatt gcgtcgtgat atgttcgccg
     4321 ggataaacca tataggatgt attgacgcca aggctgcagt atttctcttt gagattcaga
     4381 gcctgttcca gcgggataaa ttcatccccc gtgccatgat acagaaaaac gggtgcatta
     4441 atttttgttt ttccgagttc ctgaccattg accacttcag caatttccgg aatcgcaaaa
     4501 atctgatcgg aggtttcccc gtatttggtg aaatcggaaa gcggcgtatt catgaaagca
     4561 aaaagagcct cgaatacacc catggaaagg cctcgaccaa tcgcgtccat cccgtcagtg
     4621 ttcacccggc tattcagaag aatgtcattc gggtactgcg cgttcagccc gatgcaggtt
     4681 gacagcagga aggatgaacc gttgttgccg tccagatatc tcccgacaga aaacagctcg
     4741 gcaggaacac cgccggccgc aaccccggcc aatttaatac cgggtgcgta ggtcgactgc
     4801 aactggcctg cccatgccgc cgactgaccg ccctgggaat atccccagat accgactttt
     4861 gcataggact tgagagatat attggggatc tgcagagctg ccgtcacaat atcgagaact
     4921 gcctgtccct gagccttgcc caccatgtag gaaggtacat cgttgttggt gtaacccggg
     4981 ttatcggtga tgagaacggc atagcccctg ttgagggcag ccgcgatatt cgaattctcg
     5041 taatccgttc cgttttccag ttgaatcgat ggcgcccaac tctgcacaag accgtgcgtg
     5101 ccgaccgcat aactgatgat gggacgcgcg gtgaggccgt aattatataa agacttcgga
     5161 accagtaccg taccggtaac cacgttgggt tctcccaaag catcctgtga cgcatacatg
     5221 acggtccatg aataacacga tggtccgccc ggcggaacgg atgttgatgt gcggtaacgg
     5281 attaaggtgc catgcgtgcc gggtatagtg gatggcttct cataaaaagc cattccggct
     5341 ggaccctgtg tgggagcagc aactgatgaa atcgcccaaa aagctcctac catcaaaatc
     5401 aaaaaggata aaaaaaacag tcgtttatac caaaacatac gtttcataaa attcccccct
     5461 taaaattttt tttgaataat gaagaagtgc gacccataca tcaactttga catcttgatg
     5521 ttcttacatg ggccttattt ggtcagaaaa cgtgcaggcg agaatcagaa acgtaccgtt
     5581 cgaattgcct gttaaaatat tatattttat ttttttgtca agaaaaaaaa tcaaatggtt
     5641 tgataacctt aatta
//
LOCUS       DAOOLB010000115         5536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_17625_length_5536_cov_44.943441, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000115 DAOOLB010000000
VERSION     DAOOLB010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17625_length_5536_cov_44.943441"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLF77_10140"
     CDS             complement(<1..479)
                     /locus_tag="PLF77_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09256.1"
                     /translation="MNEMKKDREHWASRVGFILAAAGSAVGLGNIWRFPYMTGENGGA
                     AFIVIYLLAVFLLGYPLMVNETVLGRASQRDPVGAFKALAPGSPWWLIGALGVFTGFI
                     ILSYYIVVAGWSLAYIYKVVIAVSNPGINHEAVFKSHITSLWEPIIWAAVFMILTIG"
     gene            677..1564
                     /locus_tag="PLF77_10145"
     CDS             677..1564
                     /locus_tag="PLF77_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024878.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HPC09257.1"
                     /translation="MKLEKDLYAIDKTLLVQAGETISRKTLQRVAALSGKITYVPIKD
                     TWLMKDLVETFKDERYTNILYPPDINRKIVRSIARIRMPEKILSELAYLKTRSPYTYQ
                     HIMVIAILAGKVSFDHSLKNKYLHDLAVLLSLFHDLGKSRIPLSILDKPTPITIKERR
                     ILTSHPLIGYVLLHYYFGKHHKKYSFSSFQHHERLDGSGYPLGIKTLNKYSQLIGVVD
                     ALDALISERPYRRKPFSLRAAIDLLLDEAERGRFRKSLVRTLIIYSRRENPGAKIVIA
                     QRGRDQAPLDNAYGKTARS"
     gene            complement(1602..4841)
                     /locus_tag="PLF77_10150"
     CDS             complement(1602..4841)
                     /locus_tag="PLF77_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal domain-containing protein"
                     /protein_id="HPC09258.1"
                     /translation="MTVKRKIIIAVVVVAVVAGAAVLTRAWFYAADKVAQPDSYREYI
                     TKTVSEKMHRSIAYDKSTASLTLRNGLTFNFTHLVIREKDGASDFLRVDEALVRINIL
                     PLLRNRLVFGELVLDRPRVALKRDSSGVLNIADLLIRKEDKKAPRIRKVIIKKGAVTF
                     VDQAADPAGLVTSLENLDGRINALFWMNRYRFHITSSILENNNRASVELSGLFSPVSA
                     GKPVYESKLRASVHLKDADLKHYNAYLKMYTPIEQVSGKLKLDMTYSGRFSDFKSKGT
                     VTLRNASLYYPGVFNGYLRPRIAEANFSLKRDKKSLSVDVGRLTVDKFNAKGSFEMDD
                     LDKKDPLLEASAITTAFQLKDVTSYVPWGIIHKGVGDFIQEHIKDGKFRLIEGKLKGR
                     LSQIANINHQEGVDVLSITAEVDKGVFEVVPSAPLFNDIKGILELKRRQFSIKNIRAY
                     FGKSPCTVEGNISDFARPEPNVYTAYMKVQPVRDDVVWLLGEKTFNDMDFRGTSLLTL
                     SGKGPDTGFRIRADWNLTDVAYAFPGVLEKPRSRKNQFTADLVIDDQAVDFQSFKHVM
                     PPVNVTGNMRLRYEGDSPASFSIQSGTFDIREAAPLLPLLQKFDPTGAAQIAVTGQGN
                     LNEPSSLQWKGNIALKNISLDVSDKFSRMQGLTGSVSFKNRSMQTSLFRTQIGRSKIQ
                     GNFWIEDFSDSRVFCRVNSEALKASDLGLKSAKGDVTLRDVKGQLSFEDELIHVERLQ
                     FGAGTSTFILSGDITEFDLPKVTLELSSMYVHSEDLSRLMALSYPSKDQKAKSTVDLN
                     AVFLLDGGTFGDAPFQKLYAVMKLKNDILDVDELEVGIFDGKFKAKGRVVLPAAGPNR
                     YDANITVDKISLDKLQQYLELGDRTVTGDLSLSANISMSGANAGELKQSAMGTVKVRA
                     EKGVLKKFAVLSKIFSLLNIVQLAKLKLPDMATGGMPYSEITFHATFKGGVISSEDFF
                     IDSNAMQISGTGSIDFVRKKLDFVAGVHPLQSLDLIASKIPIAGWIITDERGKLITAH
                     FKVDGTWDDPKVSTITAKSIGKGTLDIFRRIFQLPEKLVTDTGEVILGH"
     gene            complement(4991..>5536)
                     /locus_tag="PLF77_10155"
     CDS             complement(4991..>5536)
                     /locus_tag="PLF77_10155"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPC09259.1"
                     /translation="NNLVEKAARVAAHAHWTQKRKGDDLPYIIHPVMVAVKLIKHTFP
                     DAVIAAALTHDVLEDTDYPEAKMRAELGDEVLHIVKTVTHDRSLPWEEKKKKYIETVR
                     AGSEGARAVAVSDKIHNLESLLLAYEDQGPALWKRFNRGRDKKLWFEKAMLQMLKESW
                     RHPLIDEYERLIEKMTKLKVE"
BASE COUNT         1388 a         1578 c         1222 g         1348 t
ORIGIN      
        1 atgccgatgg tcagaatcat aaatacggcc gcccagataa tgggctccca aaggctggta
       61 atgtggcttt taaaaaccgc ttcatggttg attcccggat tgctcaccgc gataaccact
      121 ttgtaaatat acgcgagcga ccatcccgcc acgacaatat aataagagag aataatgaat
      181 ccggtgaaaa cgcccagcgc gccgatcagc caccacgggc tccccggagc gagcgctttg
      241 aatgcgccga caggatcgcg ctgtgaggcg cgtcccagta ccgtttcatt gaccatcaaa
      301 ggataaccga gtaaaaaaac ggccagcaga taaatcacaa taaacgccgc tccgccgttt
      361 tctcccgtca tgtagggaaa ccgccagata ttgcccagcc cgacagcgga tcctgccgcc
      421 gccagaataa aaccgacgcg actcgcccaa tgttcacgat cctttttcat ctcattcatg
      481 gcatgctcct cgtttaatct ttcatcgaca acgccgacag ttcatttcaa catcagaccg
      541 acaaacctta tactgatctt gcctcttaaa atcaattttt tttgacgcac cgtccggaga
      601 atttttaaaa atcaccggca aagtatttga cagaaaaaac caaatcaact atgcctatcg
      661 tcatcggaga agacatatga aactggaaaa agatttgtat gccatcgaca aaacgcttct
      721 ggttcaggcc ggtgaaacca tcagcagaaa aaccttgcaa cgggtggcgg cgttatcagg
      781 aaaaataacg tatgtcccga tcaaagacac ctggctgatg aaggatcttg tcgagacctt
      841 caaagacgaa cgttacacca atattcttta tcctccggac atcaaccgga agatcgtccg
      901 ctcgatcgcc cgaatccgga tgccggaaaa gatattatcc gagctggctt atttgaaaac
      961 acgctccccc tatacatacc agcacatcat ggtcattgcc attctggccg gcaaggtatc
     1021 cttcgatcat tcgctgaaaa acaaatacct gcacgatcta gccgtactgc tgagcctttt
     1081 ccacgacctg ggcaaatcgc gcatcccgtt gagcatcctg gacaaaccga cgccgatcac
     1141 catcaaggaa cgccggatcc tgacctcgca tccgttgatc ggttatgttc tgctgcatta
     1201 ttatttcggc aagcatcaca agaaatattc tttttcttct tttcagcatc atgaacggct
     1261 cgacgggtcc ggttatccgc tgggcataaa aaccctcaac aaatattccc agttgatcgg
     1321 ggtggtggat gcgctggatg cgctcatctc ggaaaggccc tacaggagaa aacccttttc
     1381 gctgagagcc gccattgatt tgctgcttga cgaagcggaa cgaggcagat tcagaaaaag
     1441 tctggtccgc acgctgatta tttattcccg ccgggaaaac ccgggggcca aaattgtcat
     1501 tgcgcaaaga ggacgcgatc aggcgccact ggataacgcc tacggcaaga cagccaggtc
     1561 ctgaaaattt ttcttccgat gtttcgtttc ggcaaacatt tttaatgccc cagaatgact
     1621 tcgcccgtat ccgtgacgag cttttccggc aactggaaaa ttcggcggaa aatatcgagc
     1681 gtgcctttgc cgatggattt cgccgtgatg gtggacacct tcggatcgtc ccaggttcca
     1741 tccactttga aatgagccgt aattaatttt ccccgctcat cggtaataat ccacccggca
     1801 atcggaattt tcgaagcgat cagatccagc gattgcagag gatgaacacc ggcaacaaaa
     1861 tcgagcttct tccggacaaa atcaatgctg cctgtccccg aaatctgcat cgcgttgctg
     1921 tcaataaaaa agtcttccga ggatatcacg ccgcccttga acgtcgcatg aaacgtgatc
     1981 tcactgtagg gcatgccgcc ggttgccata tccggcagct tcagcttggc cagttgcaca
     2041 atattcagca atgaaaatat ttttgacaga accgcaaact ttttcaacac ccctttttcg
     2101 gcacgcacct tcacggttcc catcgcagac tgtttgagtt ccccggcatt ggcgccgctc
     2161 atcgagatat tggcggacag ggataaatct ccggtcaccg tgcgatcgcc caattccaga
     2221 tactgttgaa gtttatccag cgatatttta tcgaccgtga tattcgcgtc ataacggttc
     2281 gggcctgccg ccggcaaaac caccctgccc ttggccttga acttcccgtc aaagattccc
     2341 acttccagtt catcaacatc cagaatatcg ttcttcagct tcatgaccgc gtacagtttc
     2401 tggaacggcg catcgccgaa ggtcccgcca tccagcagga agacggcatt caggtccacg
     2461 gttgattttg ccttttgatc tttcgacgga taactcaaag ccatcagacg gctcaaatcc
     2521 tcggaatgga catacatgga actcagttcc agcgtaactt tcggcagatc gaactccgta
     2581 atatctcccg acagaataaa ggttgacgtc cctgctccga attgcagacg ctccacatga
     2641 atcagttcat cttcaaagga taattgtccc ttgacatcgc gcagcgtcac atcgcctttt
     2701 gcgcttttca ggcccagatc cgacgctttg agcgcctcgc tgttgacccg gcagaacact
     2761 ctggaatcgc tgaaatcttc aatccagaag ttgccctgaa ttttactgcg cccgatttgc
     2821 gttctaaaca gcgaggtctg catcgaccgg tttttgaaag acacactgcc cgtcagcccc
     2881 tgcatccggc tgaacttgtc ggacacatcc agcgaaatgt ttttcagagc aatgttcccc
     2941 ttccactgca acgaagacgg ctcgttgaga ttaccctgac cggtcacagc aatctgcgcc
     3001 gcgcccgtcg gatcgaattt ttgcaataac ggcagcagcg gtgcggcttc acggatatca
     3061 aacgtccccg actgaatgga aaacgatgca ggcgagtccc cctcatagcg cagcctcata
     3121 ttccccgtaa cattcaccgg cggcatcaca tgtttaaacg attgaaagtc aaccgcctgg
     3181 tcatcgatca ccagatcggc cgtaaactga ttttttctgg agcgcggttt ttccagaaca
     3241 cccggaaatg cgtaagcaac atccgtcaga ttccagtcgg ccctgatccg gaaaccggta
     3301 tccggacctt tgcccgaaag cgtcaacagc gaggtccccc ggaaatccat atcgttgaag
     3361 gttttctcgc ccagcagcca gaccacatca tcgcgaaccg gctgaacctt catgtaagcg
     3421 gtgtaaacat tgggttcggg ccgggcaaaa tcggaaatat tgccttccac cgtgcagggc
     3481 gacttgccga aataggcccg gatgtttttg atagaaaact gccttctctt gagctccaga
     3541 atgcctttga tgtcattaaa caacggcgcg gaaggcacca cctcaaagac acctttatcc
     3601 acctcggcgg taatggaaag cacatcgacg ccttcctgat gattgatgtt ggcgatctga
     3661 ctcagccggc cttttaattt cccttcaatg agccggaact tgccatcctt gatgtgctcc
     3721 tgaatgaaat cccccacgcc tttgtgaata atgccccacg gaacatagga tgtaacgtct
     3781 ttcaactgga acgcggtggt gatggccgac gcttccaaaa gcgggtcctt cttatccaga
     3841 tcgtccatct caaagctgcc cttcgcgttg aatttatcaa ccgtcagcct tccgacatcc
     3901 acgctcagac ttttcttatc gcgtttcaaa gaaaaattcg cttcagcaat ccgcggccgg
     3961 agatacccgt taaaaacgcc ggggtaataa agagacgcat ttctcagtgt gaccgtcccc
     4021 ttcgatttaa aatccgaaaa ccgccccgaa tacgtcatat ccagctttaa tttgccggac
     4081 acctgctcga taggcgtgta cattttcaga tacgcattgt aatgttttaa gtccgcatcc
     4141 tttaaatgaa cagacgcccg caatttactt tcatacacgg gctttcctgc agagacggga
     4201 ctgaacagac cgctcaattc caccgacgcc ctgttgttgt tttccagaat agacgatgtg
     4261 atatgaaaac ggtatcggtt catccagaac agcgcgttaa tccggccgtc gagattttcc
     4321 agcgacgtca caagaccggc cgggtccgcc gcctgatcca caaaagtgac cgcccctttt
     4381 ttaatgatga ctttccggat tctcggcgcc ttcttatctt ctttgcgtat cagaagatcc
     4441 gcgatgttca gcacgccgga gctgtcgcgc tttaaagcaa cacggggacg atcgagaaca
     4501 agctccccga aaaccagacg gtttctcaaa agcggcagaa tattgatccg gaccagcgct
     4561 tcatccaccc ttaaaaaatc ggacgcgccg tccttttccc ggatgaccag atgggtaaaa
     4621 ttaaacgtca acccgttgcg caacgtcagc gaggccgtac tcttgtcata agcaatgctg
     4681 cgatgcatct tttcgctgac ggttttggtg atgtattccc ggtaactgtc cggctgcgca
     4741 accttatccg ccgcataaaa ccaggcgcgc gtcaacaccg cggcaccggc aaccaccgct
     4801 acaacaacga cggcaatgat aatttttctt tttacagtca atgaagggcg cctcttttcg
     4861 aaatcctata gctttttgta caataaccct tgatggctgt caattttaag atttatgctc
     4921 ttgcgacgag aaattcgatc tgtttttcag aaaatacgtt ctttggggga aacggcgtgt
     4981 tccccggcgg ttactctacc ttcaacttgg tcatcttttc gatcaggcgc tcgtattcat
     5041 cgatcaaagg atgccgccag ctttctttaa gcatttgcag cattgctttt tcaaaccaga
     5101 gcttcttatc cctgccgcga ttaaaccgtt tccacaacgc cggcccctga tcctcatacg
     5161 ccagcagcag actttccaga ttgtggattt tatcggacac ggcaacagcc ctggcgcctt
     5221 cggaacccgc acgcacggtt tcaatatact tcttcttttt ttcctcccac ggcaaagacc
     5281 ggtcatgggt taccgttttc actatgtgca gcacctcatc gccgagttcc gcccgcatct
     5341 tcgcttccgg ataatccgta tcttccagaa catcatgcgt taatgccgcg gcgatcaccg
     5401 catccggaaa cgtatgtttg attaacttca ccgccaccat caccggatga ataatgtagg
     5461 gaaggtcgtc gcccttgcgc ttctgagtcc aatgcgcatg ggccgccacc cgcgccgcct
     5521 tttcaacaag attatt
//
LOCUS       DAOOLB010000116         5527 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_17674_length_5527_cov_45.215278, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000116 DAOOLB010000000
VERSION     DAOOLB010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17674_length_5527_cov_45.215278"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1899
                     /locus_tag="PLF77_10160"
     CDS             <1..1899
                     /locus_tag="PLF77_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPC09260.1"
                     /translation="LEAIKKTDCLVIAPTGSGKTWIAREAILSVLEKGGRAWYASPLK
                     ALSNSKWIEFGLHFDTENVGIITGDTKENTEAPIIVGTTEILRNQLYDAMHQGKDLNC
                     DLVILDEAHFLGDTDRGVVWEEIMIYLPARIHLLLLSATIGNGEEIAHWLESIRKKSC
                     AVVAEDKRPVPLYPLFLHPSGCLHPFLEGKKVSPPVADFIRRTDEKRLRGGKMPDYIG
                     IIRVLEKFNLLPAIFFLKSRAECDAALTARGMLQPLQNATVFSYSLDELLGRFPTLAR
                     HRQLKVLRSMGLASHHGGQLPAWKMLVEEMMNRGHLRVIFATSTIAAGVNFPARTIVL
                     FNSDLFNGSDFSPLTATEFRQMTGRAGRRGQDNIGFMLTVTGKFMNLNHIRGLLFQKP
                     EDILSRLKNDFAMVLNLLLSQTPDDVRKIFERSLAAYQQNSRHESADFSAAQSLWKDF
                     SRHLKFLKQEGFVDESGALTDDGCWASKLRLDYPLLVAQCLRENAFPQDNEKILAALV
                     ALLAYDRDNDMKMTVKDLPPKLALAFKKVMLAVRPLIHRMENAGFAPARLHTLAGVAM
                     YLWAQGYGWDSVIKATGIAEGDMATLVLRTADNLRQIASLKDTYPDIAGCAQRARETI
                     LREPVLFL"
     gene            2259..2576
                     /locus_tag="PLF77_10165"
     CDS             2259..2576
                     /locus_tag="PLF77_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09261.1"
                     /translation="MWLYLKTGFYSVVTKEPCKEDELLVRAHSKDDLDKLQNLLKKQC
                     QFHGEVLDTPQTDYAYRMIVPKETFASFMSRAINDLVYEEYKNTKSWEAAYRWQSNLQ
                     RSK"
     gene            2647..4626
                     /gene="uvrB"
                     /locus_tag="PLF77_10170"
     CDS             2647..4626
                     /gene="uvrB"
                     /locus_tag="PLF77_10170"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF003673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPC09262.1"
                     /translation="MITDFVPAGDQPQAIDRLVQGLEKGNGHQVLLGVTGSGKTFTIA
                     NVIERVQRPALIIAHNKTLAAQLYEEFKGLFPENAVEYFVSYYDYYQPEAYLPVTDTY
                     IEKDSAINEEIDKLRHAATHALLTRRDVIIVASVSCIYGLGSPEAYLGMIVQLEAGKE
                     FSRDELLKRLIEIQYERNDIDFHRGTFRVRGDIVEIFPPYEDEQALRIEFFGDMIESM
                     TLIDPLRSKKIGVVHKTAVYPGSHYVSSRENLQRAIHDIRRELVLTLAEYRDQNKLLE
                     TQRLEQRTNFDLEMMEEMGYCQGIENYSRHLTGRKPGQPPPTLMEYLPQDALIIIDES
                     HATLPQLAGMYRGDRSRKETLVKYGFRLPSALDNRPLRFDEYEALPNQRIYVSATPAQ
                     YEMAKAKGIRVEQIIRPTGLMDPEIEVKPATNQVDDLLAEIGARIEKNEKVLVTTLTK
                     RMAENLTDYYAGLDIKVRYLHSDVHTLERVDIIRDLRRGVFDVLIGVNLLREGLDIPE
                     VSLVAILDADKEGFLRSERSLIQTSGRAARNVAGKVIMYADKMTRSIQACLDETKRRR
                     TIQQKYNEENNITPETIKKSISSVLGSIYEADYVTLPATVSERVAPLKEEDLPAFIAK
                     LTREMKQAAKNMEFEHAAELRDEIRELNKILLEMG"
     gene            4741..5199
                     /locus_tag="PLF77_10175"
     CDS             4741..5199
                     /locus_tag="PLF77_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09263.1"
                     /translation="MKALKSILCVLTLFVFVFAGCEKEASDWVQYHKDVDGNVYLYDQ
                     NRVKVDAANYTVEVWAKQVYSDTGRTIELQSRIKDGLSMKGYDKLAHKTCRYAIDCAK
                     KGITVLSISHFDSDGESLYFDVDKGEKKMFIINPGSPAAALQKEVCSKKQ"
     gene            5296..>5527
                     /locus_tag="PLF77_10180"
     CDS             5296..>5527
                     /locus_tag="PLF77_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09264.1"
                     /translation="MNKVVARLANGKMIKGTTADFFPGKDVFHVTESAAAPGTKALEV
                     FTKDMKALFFVKDLAGNPQHVKTNEFNASQPVV"
BASE COUNT         1490 a         1267 c         1452 g         1318 t
ORIGIN      
        1 ttggaggcaa tcaaaaaaac cgattgtctg gtcatcgcgc ccacgggttc aggaaaaaca
       61 tggattgcgc gggaagccat tttatccgtc ctggaaaaag gcggacgtgc ctggtacgcg
      121 tcgcctctga aagcgttatc caattccaaa tggattgaat tcggcctgca tttcgatacg
      181 gaaaatgtcg gtatcatcac cggcgatacc aaagaaaaca ccgaggcgcc gatcattgtc
      241 ggcactaccg aaatcctgcg caatcaactc tacgatgcca tgcatcaggg aaaagacttg
      301 aactgcgatc tggtcattct ggacgaggcg catttcctgg gcgacaccga tcgcggcgtt
      361 gtctgggaag aaatcatgat ttatcttccc gcgcggattc acctgctgct tttgtcagca
      421 accatcggta acggcgaaga aattgcacac tggctggaat ccattcgcaa gaaatcctgc
      481 gctgtcgttg cggaagacaa acgtccggtg ccgctttatc ccctttttct gcatccatcc
      541 ggttgtcttc atccttttct ggaagggaaa aaagtatccc cgcctgttgc cgatttcatc
      601 agaagaaccg atgagaaaag attgcgcggc gggaaaatgc cggattacat cggcatcatc
      661 cgggtactgg aaaagttcaa tctgctgccg gcaatatttt ttctgaaatc gcgcgcggaa
      721 tgcgatgcgg cgctcactgc gcgcggaatg ctgcagccgc ttcagaatgc cacggtgttt
      781 tcatattcac tggatgaact gctgggacgt ttccccacgc tggccaggca ccggcaattg
      841 aaagttttaa gatcgatggg actggcgtct catcacggtg gacagctgcc cgcgtggaaa
      901 atgctggtgg aagaaatgat gaacagagga catctgcgcg tgatctttgc aacatccacc
      961 attgccgcgg gcgttaattt cccggctcga accatcgtcc ttttcaactc cgatttgttc
     1021 aacggcagtg atttcagtcc gctgacggct acagaattcc gccagatgac cggacgcgcg
     1081 ggacgccgcg ggcaggacaa catcggcttc atgctgacag ttaccgggaa attcatgaac
     1141 ctgaatcaca tccggggttt gttgtttcaa aaaccggagg atattttaag ccggttgaaa
     1201 aacgatttcg caatggtcct gaatctgctt ttgtcccaga cgccggacga tgtccgcaag
     1261 atattcgagc ggtcgctcgc ggcatatcag caaaacagcc ggcatgaaag cgcagacttt
     1321 tccgcggccc agtcgctctg gaaagatttt tcccgccacc tgaaatttct gaagcaggaa
     1381 ggatttgtgg atgaatccgg agcgttgact gatgacggat gctgggcgtc aaaactgcgt
     1441 ctcgattatc cgttattggt ggctcagtgc ctgcgggaaa acgcgtttcc tcaggacaat
     1501 gaaaagattc tggccgctct tgtggcgttg ttagcctatg atcgggacaa tgatatgaaa
     1561 atgactgtaa aagatttgcc gccgaagctt gctcttgcgt ttaaaaaggt catgctggcc
     1621 gtccggcctc tgatccatcg gatggaaaac gccggatttg cgcccgccag actccacacg
     1681 ctggcgggcg tggccatgta tctctgggcg cagggatatg gctgggattc cgtcattaaa
     1741 gccaccggaa tcgctgaagg cgatatggca acgctggttt tgcgcacggc ggataatctg
     1801 cggcagatcg catcgcttaa agatacatat cccgacattg ccggatgcgc gcaaagggca
     1861 agagaaacga tcctcaggga gcccgttctc tttctttaaa gagatctgtt gaaagatcgc
     1921 attctctgaa cgtcgccacg ttagatatcg ttttgtatag atcgcttggt ccatgtttcg
     1981 cagatgcgta tgatcccgct cttgcacgcc catggtgtca gtattttttg aacagtccat
     2041 aaaagtgaaa aaaatttcag ccgtcacggc agagcgatga ccggtgacgc gtatcttttg
     2101 attcaacttt cattttcaaa atattgattt tatgataaac ttaatacgtt tgaatttgaa
     2161 attgaaatga tcatccatga ttagtgaaga tctgaagacg ttttttttaa cgggctagcg
     2221 cgtgtatttg ccattgagga gaaacgcaag gggaaacgat gtggctttat cttaaaacag
     2281 gtttttattc ggtagtgacc aaggaaccgt gcaaagaaga tgagctttta gtcagggcgc
     2341 acagtaaaga tgatctggat aaattacaga atttactgaa aaaacagtgt caatttcacg
     2401 gcgaggtgct ggatactccc caaaccgatt atgcataccg tatgatcgtt cccaaagaaa
     2461 catttgcgtc atttatgtcc cgcgccatca acgatctggt ttatgaagaa tacaaaaaca
     2521 caaaaagctg ggaagccgct tatcgctggc agagtaattt gcagcgaagt aaataagcca
     2581 tccgcttctt cgcgcagcaa caaatccgat catttaaaac aatcatagca tatgaagaaa
     2641 ctttcaatga taacggattt tgttccggcc ggtgaccagc ctcaggcgat tgatcgtctg
     2701 gtgcaaggtc tggagaaggg caatggacat caggtccttc tcggggtaac cggttccggc
     2761 aagacattta cgattgccaa tgtcatcgaa cgcgttcagc gtccggcgct gattatcgcg
     2821 cacaataaaa cgctggcggc gcagttgtat gaagaattca agggactctt tcctgaaaac
     2881 gccgtggaat attttgtcag ttattatgat tactaccagc cggaagccta cctgccggta
     2941 accgatacgt atatcgaaaa ggattcggcc atcaacgagg aaatcgataa actgcgtcat
     3001 gcggcaaccc atgctctttt gacgcggcgc gatgttatta tcgtcgccag cgtttcctgt
     3061 atttacggtt tgggatcgcc ggaggcctat ctgggaatga tcgtccagct ggaagcgggc
     3121 aaggaattct cacgggatga actgctgaaa cggctgattg aaatccagta tgagagaaac
     3181 gatatcgatt ttcatcgcgg gacgttccgc gtccgcggcg atatcgtgga gatctttccg
     3241 ccttatgaag acgaacaggc cttacgaatc gaatttttcg gtgacatgat cgaatcgatg
     3301 acgttgatag atccgttgcg aagcaaaaag ataggcgttg tccataaaac tgctgtttat
     3361 cccggaagcc attatgtttc ttcccgtgaa aatctgcaac gggcgattca tgatattcgc
     3421 cgggagctgg tactgacgct tgccgaatac agagaccaga ataaactgct ggagacgcaa
     3481 aggctggagc agcgcaccaa cttcgatctg gaaatgatgg aggagatggg gtattgtcag
     3541 ggtatcgaaa attattcccg tcatttaacc ggacgcaaac ccggtcaacc gccgccgaca
     3601 ttgatggaat atctgccgca ggatgccctg attattattg atgaaagcca cgcgacactg
     3661 ccgcaactgg ccggtatgta ccgcggcgac cgttcccgca aggagacgct ggtcaaatat
     3721 ggattccgcc ttccctccgc gctggacaac cgtccattgc gttttgacga atacgaagcc
     3781 ctgcccaatc agcgcatcta tgtttcggcg acgccggcgc agtatgaaat ggccaaagcg
     3841 aaaggtatcc gtgtcgagca gatcatccgt ccgacaggat tgatggatcc ggagatcgaa
     3901 gtcaaaccgg ccacgaatca ggttgacgat cttctggcgg aaatcggagc gcggattgaa
     3961 aaaaatgaaa aagtgctcgt gacgacgctg acgaaacgca tggcggaaaa tctgacggat
     4021 tactacgccg gtctggatat caaggtacgc tatcttcact ccgatgtgca cacgttggag
     4081 cgggtggaca ttatccgcga tcttcgtcgg ggcgtttttg acgtcctgat cggtgtcaac
     4141 ctgctgcggg agggattgga cattcccgaa gtgtcgttgg tggccattct tgatgcggac
     4201 aaagagggat tcctgcgatc ggaacgttca ctcatccaga ccagcggccg cgccgcgcgc
     4261 aatgtcgccg ggaaagtcat catgtatgct gataagatga cccgctcgat tcaagcctgt
     4321 ttggatgaga caaaacggcg ccgcaccatt cagcagaaat acaatgaaga aaataacatc
     4381 acccctgaga ccattaaaaa atcgatcagc agtgttctgg gatcgattta cgaagccgat
     4441 tacgtaacgt tacccgcaac cgtgtcggaa agagtcgcgc cgctaaaaga agaagacctt
     4501 ccggctttca ttgcaaaact gaccagagaa atgaagcagg ccgcaaaaaa tatggaattc
     4561 gagcacgccg ccgaactgcg ggacgaaatc agagagctga acaaaatatt gctggaaatg
     4621 ggttagttct actttccttc aatatatggt ataaatattt attgccgatg tgtgttgaga
     4681 acattcgatt cagggaatta cggcaggatg aatgaatcgg tcacaaaaaa ggagtgaagc
     4741 atgaaagcac tgaaatcgat attatgcgtg ttgacgttat ttgtttttgt atttgccggt
     4801 tgcgaaaaag aggcgtccga ttgggttcag taccataaag atgtcgacgg gaatgtttat
     4861 ttgtacgatc agaacagggt aaaagtggac gcggcaaatt atacggtcga ggtctgggca
     4921 aaacaagtct attccgacac gggcagaacg atagaactac agtccagaat caaggatgga
     4981 ttatcgatga aagggtatga caagcttgcg cataaaacat gccggtatgc gattgattgc
     5041 gcaaagaagg gcataacggt attatccatc agccattttg acagcgacgg agagtcgtta
     5101 tatttcgatg tcgataaagg tgaaaagaaa atgtttatta tcaaccccgg ctctccggcg
     5161 gcggcccttc aaaaagaagt ttgctcgaag aaacagtgat cctgttgcga accgggggca
     5221 gaaatgatca ttggtaattg cccggacgca gagattgaaa tgaatgtctg atgaaattat
     5281 tgaagaggaa tgcttgtgaa taaagtagtg gcacgattgg caaatggaaa aatgatcaag
     5341 ggaacgaccg cagatttctt tccgggaaaa gacgtttttc acgtaaccga atcggcggcc
     5401 gcgccgggaa ccaaggcgtt ggaagtgttc acgaaggata tgaaagcgtt attctttgtg
     5461 aaagatctgg caggcaatcc gcagcacgtc aaaaccaacg agtttaatgc atcgcaaccg
     5521 gttgtcg
//
LOCUS       DAOOLB010000117         5486 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_17833_length_5486_cov_35.440987, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000117 DAOOLB010000000
VERSION     DAOOLB010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5486
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17833_length_5486_cov_35.440987"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PLF77_10185"
     CDS             <1..573
                     /locus_tag="PLF77_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC09265.1"
                     /translation="KPFGREEVVEALIASASELFARHGVNGVSLRTITEHAGVNLGLF
                     HRHIGTKENLFRLTLQHMAQQNFGELQAGATLFEVVQHAFSSLDRDTGFWRIMARSLL
                     DGAQPEDLHTHYPVADHLLKLAQQAVDEGTLTKDIDPRLLVAAMYAFALGFKLFEPFI
                     IKATGLDDLDPEEVKTRIVKIAATLVSPRQ"
     gene            845..2866
                     /locus_tag="PLF77_10190"
     CDS             845..2866
                     /locus_tag="PLF77_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HPC09266.1"
                     /translation="MKTGRDVHKILCQMSGGLKTLSIITIIFLLTGTVTGCGGRYSGI
                     ASATSEVTPSTQQANDSFAQTLNLDDPQDFEDARRGFIARPNGKIINSKGDVVLDFDA
                     FQFLEGKSPQTVNPSLWRHALLNAQIGLFKVAEGIYQVRGFDIANMTLIEGSNGWIVV
                     DPLSCRETAEAAIAFARKHLGNKPVSAIVFTHSHIDHFGGVFGVTSIQDVASRKIPVV
                     APDGFLEEATSENIMAGPAMERRSMYQFGKNLDRSAKGMVDAGIGKSIAYGTYGILQP
                     NLIVTRAKQELVIDGVRFVFYNVPKSESPAQMTFVLPDKKAYCGAEILTQTMHNILTP
                     RGAKVRDALRWSNYLDQALEQTADVEVCFGTHNHPVWGRARISEFITKHRDVYRYTHD
                     QTVRLFNAGYTPREIADMIKLPQSLESYFGCRGYYGDLRHNVKAVYQFYLGFYDGNPA
                     NLNPLTPLESGKRYLDLAGGADRLIEAAQAAFDKGDYRWSIELLNHVVFAQPGNKNAI
                     ALMARTYDQMAYMSESSVWRNIYLTAASELRNGPPKRESDRSYAIDMLMQTPIESFLD
                     AMAAALNGPAAEGKAFKVNLVISDAQESFVLWIENAVLHHRKTAPAPDANVTITMKKD
                     VFIKLLTDSADIKDTLLSSDFKITGSRIDLIRFLTLFDKPPVSFAVVTP"
     gene            complement(3016..3345)
                     /locus_tag="PLF77_10195"
     CDS             complement(3016..3345)
                     /locus_tag="PLF77_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09267.1"
                     /translation="MVTVQLKDGAAFNPDDVYAWYVKQQKEGGMDPKWMPDYIRIIDK
                     FTVTDTQKVIVRPFKRENFNIDKNPDMQIYFKKRGEATYHKLTPEEFNKIKDDFKTNG
                     RQTILCS"
     gene            complement(3356..4030)
                     /locus_tag="PLF77_10200"
     CDS             complement(3356..4030)
                     /locus_tag="PLF77_10200"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HPC09268.1"
                     /translation="MLTYGVSFLNYVGQPLHYIIDALERKYGSGEAIEKALANDPRNK
                     FRKAYGNGASVNDRIKLKRYLNMYHIYEIYGSTEAVITTANRPGDPIDSVGEVDSSVL
                     ILDEEGRECDPGIVDANGALTNYDKAVGEICRKVGDNNLRFEGYYGNQGATSQKFRNG
                     WYHSGDLGHVRIVNGKRYMFFNGRTDDWIRKDGENFFSRECSRLCSECSVRGLRHCVW
                     RPLRGG"
     gene            complement(4003..4797)
                     /locus_tag="PLF77_10205"
     CDS             complement(4003..4797)
                     /locus_tag="PLF77_10205"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HPC09269.1"
                     /translation="MTMWWLWFRRDRVFFIFEGREYTYREVYRQSRRYEKFFLSMRKK
                     LVDSGKLGRDQRLAFGVYMDNNPEFVFASLGAGLSNSIMFAINSGFRGETLDKVMEQA
                     GIAFLVINDSTLKEVEGVVKSGSVIGPEKVYFVGDEKPFKSNGFKPLEKAVEEAQKTD
                     VPGSYRVKVDNFSAVIVIYTSGTSGLPKGVPCTHIKLFGAGFVVQSAVHLTKDERGYI
                     SMPLFHSNAWYIGILPQMIVGGSFVLKRKFPQARLKKICLPTGFPF"
     gene            5143..5430
                     /locus_tag="PLF77_10210"
     CDS             5143..5430
                     /locus_tag="PLF77_10210"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HPC09270.1"
                     /translation="MITLIATLRIKEGQTQAAIDVLKEIVPKIKASEPGCLEYIPHTV
                     RGDEKTIIFYEKYSDKDALKLHSANLMKNMESLFPLLEPGMDVKTCYEIIP"
BASE COUNT         1405 a         1427 c         1283 g         1371 t
ORIGIN      
        1 aaaccattcg gtcgtgaaga ggtcgttgaa gctttgattg cttccgcctc ggaactcttt
       61 gcgcgacacg gggtgaacgg cgtgtccctc cgaaccatca ccgagcatgc cggggtgaat
      121 ctgggactgt tccatcgtca tataggcacc aaggaaaatc tattccgttt gaccctccag
      181 cacatggcac aacagaactt tggtgaactc caggccgggg ctacgctttt cgaagtggtt
      241 cagcatgcct tcagttcact cgatcgggat accgggttct ggcgcattat ggcacgcagc
      301 cttttggacg gcgcacaacc cgaagacctc cacacacact accccgtggc tgatcatttg
      361 ctgaaactgg cgcagcaagc cgttgatgag ggcacattga cgaaggatat cgacccgcgt
      421 cttctggtcg ctgccatgta cgccttcgcg ctgggcttca agctcttcga gccattcatc
      481 atcaaggcca ccggattgga tgatctcgat ccggaggagg ttaaaacccg cattgtcaag
      541 attgccgcca ctctcgtttc gccccgccaa taataagaaa ccacaggcca ttccatcaga
      601 ttagcgccgc ccgcccggaa ctgccaatgc tttcgtctca caggcatcag ggggcttccg
      661 cacatcggag gcacaggtga gaaagttgtt gcttgacaaa gtgtatacaa gtgtgtacac
      721 tactggccat attgctggac ccgagagggg aggtgaagct tgcaagaaat tgatttctat
      781 gttttttctc ggtattacca agagaaaata gttcaaattc atcatcataa aatcaggagg
      841 aatcatgaag acaggccgtg atgttcataa aattctgtgc cagatgagcg gtggattgaa
      901 gaccttgtca atcataacca ttattttttt actgacagga accgtcaccg gttgtggcgg
      961 aagatattcc ggtattgcta gcgcaaccag tgaagtaact ccttcgacac agcaggcaaa
     1021 cgatagcttc gcacaaacac tcaatctgga cgatccacag gattttgaag atgccaggcg
     1081 aggttttatt gccagaccca acgggaaaat catcaactcg aaaggtgacg tggtgctcga
     1141 tttcgatgcc ttccagtttt tagagggaaa gtccccgcaa acggtcaacc ccagcctctg
     1201 gagacatgcc ctgctgaacg cccagattgg tttgttcaaa gtggcggaag gcatctacca
     1261 ggtacgtggc ttcgatatcg ccaatatgac cttgatcgaa ggcagtaacg ggtggatcgt
     1321 tgtcgatccg ctgtcctgca gggaaacggc agaagcggcc atcgctttcg cccgcaagca
     1381 cctaggcaac aagcccgtct cggcgattgt atttacgcac agccatattg accattttgg
     1441 aggtgtgttc ggcgtgacgt cgattcagga tgtggccagc cggaagattc ccgttgtcgc
     1501 tccggatggt tttcttgagg aagccaccag tgaaaacatt atggccggac ccgccatgga
     1561 gcgtcgttca atgtatcagt ttggaaagaa tcttgaccga tccgccaaag gaatggtcga
     1621 tgcgggaatc ggtaaatcaa tcgcgtatgg cacatacggc atcctgcagc cgaacctgat
     1681 cgtcacccgg gccaaacagg aactggtcat cgatggcgtg cgtttcgtct tctacaatgt
     1741 gccgaaatca gagtctcccg cccagatgac ttttgtgctt cctgataaaa aggcctattg
     1801 cggggctgaa atcctgaccc agaccatgca caacatttta acaccgcgcg gcgccaaggt
     1861 gagggatgcc ttgcgctggt caaactatct ggatcaagcc ctggaacaaa cagccgatgt
     1921 cgaggtgtgt ttcggtacgc acaaccatcc ggtctggggt cgggcgcgca tcagcgagtt
     1981 catcaccaaa catcgtgatg tctaccgcta cactcacgac cagaccgtgc gcctcttcaa
     2041 tgccggatat actccgcggg aaatagccga catgataaaa ctgccccagt cattggaatc
     2101 ctatttcgga tgccggggct actacggtga cttgcgacac aacgtcaagg cggtctatca
     2161 attctacctg ggattttatg atggcaatcc ggccaatctg aatccgctga caccgttgga
     2221 atcgggcaag cgctacctcg atctcgcagg gggcgccgat agactcatcg aggcggctca
     2281 ggctgccttt gacaaagggg actaccgttg gtcgattgaa ttgctgaatc acgttgtgtt
     2341 tgcacaaccc ggcaataaaa acgctattgc gttgatggcg cgcacctatg accagatggc
     2401 ctatatgtct gaatctagcg tctggcgtaa catttatctg acagccgctt cagagttacg
     2461 caatggtccg ccgaaacggg aaagcgatcg cagttacgca atcgacatgc tcatgcagac
     2521 accgatcgaa agttttctcg acgccatggc tgccgcgctc aacggcccgg ctgccgaagg
     2581 aaaggccttc aaagtcaacc tggtcatctc ggatgcacag gaatccttcg tgctctggat
     2641 agagaatgcg gtactccacc ataggaagac agccccggca cctgatgcca atgtgacgat
     2701 taccatgaaa aaggacgtct tcatcaagtt attgaccgac tccgccgaca tcaaggacac
     2761 cctgttgagc agtgatttca agatcactgg cagccgtatc gatctgatca gatttctcac
     2821 tctgtttgac aaaccacccg tttcgtttgc agtcgtaacg ccgtaatcag gcgaccttgc
     2881 acggacacgc ggtggatggc ttttgcggcc attcaccgca cgcaaaccaa ccgggctttg
     2941 gataaatgga gggatgctgt atgttgaatc cggcagcagg ccgggatgtt tgcgcacgct
     3001 gccggattca gtttcttaac tgcagagaat tgtctgacga ccgttcgtct tgaaatcatc
     3061 ctttattttg ttgaactcct ctggcgtgag tttgtgatag gttgcctcgc cgcgtttctt
     3121 gaaataaatc tgcatgtcgg gatttttgtc aatattgaaa ttctctcgtt tgaaaggccg
     3181 cacaattact ttctgtgtat cggtaacagt aaacttatca atgatgcgta tataatcagg
     3241 catccacttg ggatccatcc cgccttcttt ctgctgtttt acataccatg catagacgtc
     3301 atcgggatta aacgcggcgc cgtccttcag ctgaaccgta accataacct tttcatcagc
     3361 cacctcgcag ggggcgccat acgcaatggc gcaatccacg cacggaacat tctgagcaaa
     3421 gccgagaaca ttctcggctg aaaaaattct ctccgtcctt gcggatccag tcatcggtgc
     3481 ggccgttgaa aaacatataa cgcttgccgt tcacaatcct gacgtgcccc agatcgcccg
     3541 aatgatacca gccgttgcgg aacttctgac tggtcgctcc ctgattgccg taataccctt
     3601 cgaatctgag gttgttatcg cctaccttgc ggcagatttc accaaccgcc ttgtcataat
     3661 tggtgagagc gccgttggcg tctacaatac cgggatcgca ttcacgtccc tcttcgtcca
     3721 ggatgagcac ggaggaatcc acctcgccta ccgaatcaat cgggtctccg ggacggttgg
     3781 ctgtggtaat gaccgcttcg gtggaaccgt aaatctcata aatgtgatac atgttcagat
     3841 agcgtttgag ttttatacgg tcgttcaccg aggcgccgtt gccgtatgct ttgcggaact
     3901 tattgcgagg atcgttagcc agtgcttttt ctatcgcttc accgctgccg tatttgcgtt
     3961 ccagagcgtc aataatataa tgaagcggct ggccgacata attcagaaag gaaaccccgt
     4021 aggtaagcat atcttcttca aacgcgcttg cggaaacttt cttttcagca caaaactgcc
     4081 gccaacaatc atctgcggca gtattccgat ataccaggca ttggagtgaa acagcggcat
     4141 cgaaatgtaa ccgcgctcgt ctttggtgag gtgaaccgcc gactgcacaa caaagcccgc
     4201 tccaaacaat ttgatatgtg tgcagggcac tccttttggc agacctgacg tacccgacgt
     4261 atagataaca atcaccgcac tgaaattatc caccttgacc cgatagcttc cgggcacatc
     4321 cgtcttttgt gcttcttcaa ccgccttttc cagcggcttg aatccatttg atttaaaagg
     4381 cttttcatcc ccgacgaagt aaaccttttc cggaccgatc accgacccgc tttttaccac
     4441 gccttccact tccttcaaag tgctgtcatt gataaccaga aacgctatgc ctgcctgctc
     4501 cataactttg tccagagttt cgcccctgaa tcccgaatta atcgcaaaca taatagaatt
     4561 actgagaccg gcgcctaagg aagcgaaaac aaattcaggg ttgttatcca tgtaaacgcc
     4621 gaaagcgagc ctctgatcgc ggcccagttt gccgctgtcc acaagtttct ttctcatgct
     4681 caggaaaaat ttctcataac gccgggattg tcgataaact tcccgatagg tgtattcacg
     4741 accttcaaat ataaaaaaaa cgcggtcacg cctgaaccag agccaccaca tagtcatctt
     4801 tatcatgctg ccgaaattta caaagcccaa aaacttccgt attattgttt taagattcat
     4861 tttacccaat tccccccatg attattgtta tatgtcataa taaactttaa cgcatgatga
     4921 ctttgcgatt tacctgacgg tttctttcgt aagctccttc aacggttttt attgaaattg
     4981 attaattgat aagaacttgc ttaaaaaaac cggactatat taatttaatt gtattaaatt
     5041 aatatagtta aattattaag tcaataaaaa aaattattcc tttgacacac tccgtgcttc
     5101 tccatatact cttccgggta aatataaaca aggagagcat atatgattac actcattgca
     5161 acgttaagaa tcaaagaagg acaaacacaa gcagctatcg atgtcttaaa agagatcgtt
     5221 cccaaaatta aagcaagcga gccgggttgc ctcgaataca taccccatac tgttcgcggc
     5281 gatgaaaaaa ccattatttt ttatgagaaa tacagcgata aggatgccct gaaactgcat
     5341 tccgcaaacc tgatgaaaaa catggaaagt cttttccctt tactggagcc gggtatggat
     5401 gttaaaacct gctacgagat catcccgtaa ctctacatta tggaggcatg atattctcga
     5461 aggagagaga tcgtgctccc tactgc
//
LOCUS       DAOOLB010000118         5461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_17947_length_5461_cov_36.526637, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000118 DAOOLB010000000
VERSION     DAOOLB010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17947_length_5461_cov_36.526637"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="PLF77_10215"
     CDS             complement(<1..339)
                     /locus_tag="PLF77_10215"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPC09271.1"
                     /translation="MDRNDYILSIITPVYCPNQYLIDLTEKLFLESLAMSPMKNDFEI
                     IIIDDASPKQHELKAMVEKTAREKALNIILIRNEINQGLAKSINRGVHQSRAPYILMT
                     NNDIYAPKDAI"
     gene            complement(481..1530)
                     /locus_tag="PLF77_10220"
     CDS             complement(481..1530)
                     /locus_tag="PLF77_10220"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HPC09272.1"
                     /translation="MKKSLRMAVIGMLTVAAAAQSSIASNNQARSSETLTAYMENDLF
                     EHLDRYYTHGTKFSWISRDLSNYREITSIPHWMQSIIEKVPLVNDPDQQRSVSLSFGQ
                     NIYTPEHKEKRKLLKNDRPYAGITYLGLGLHSKNTKHMDTLELGIGIVGRHSYAEDIQ
                     TKIHEWTDSEVARGWKNQLRDEPILNVYLERKWRLLQLRNSSGLGLDLIPHTGLAIGN
                     ALTGANLGGQVRFGWNVPNDFGTYLIRPGSDSSAPLDDTDPRFFAPLRRFGVHAFFAV
                     DGKAVARNILLDGNTFRDSHSVKKEPFVAEIIGGLGIIIHRIKITYSYVHRTKEFEKQ
                     KDAQHFGALALSFTF"
     gene            complement(1547..2959)
                     /locus_tag="PLF77_10225"
     CDS             complement(1547..2959)
                     /locus_tag="PLF77_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HPC09273.1"
                     /translation="MNKKIILLLVVMFFLANGCTMAPQYSRPDAPVPSEWPTGPAYEE
                     TKPSPSVPAAMELPWREFIPDENLRKVIEAALNNNRDLRIAALNVERARAYYGIQRAE
                     LLPSVNAAGSMYKERIPADLSSTGSAYTAERYDVNLGIASWEIDFFGRIRNLKDAALE
                     QYLATEEARRSAQIMLVSSIAQVYMALAADKEAFQLVTSTLKTQEDFYRLIKKRADVG
                     MASELDLRRAQSQVDSARGDFARYTQLVAQDENALNLLVGSPVSPELLPADLKNVTPP
                     RDISAGLSSAALLSRPDILAAEHRLKGTNANIGAARAAFFPRITLTASAGTASDELSG
                     LFKSGSGTWLFSPQIVVPIFDARTWSAYEVTKVEKEISIAQYEKAIQTAFREVADALA
                     ERGTVNQRLAAQLSLVDAMAVTYQLSEARYNKGIDSYLSVLDAQRSLYGAQQGLIMLR
                     LAGINNLITLYKALGGGQDN"
     gene            complement(2961..>5461)
                     /locus_tag="PLF77_10230"
     CDS             complement(2961..>5461)
                     /locus_tag="PLF77_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPC09274.1"
                     /translation="RTPEEFGAIPIRTNPDGSIIRMRDIGRTELGTEIYDIQVEHNGK
                     PSTGLAIRQAAGANALDTADRVKAKLAELSKFFPGGLKVVYPYDTTPFIRVAINEVVK
                     TLFEAILLVFLVMLLFLGNIRATLIPTIAVPVVLLGTFAILGLFGYSINMLTMFAMVL
                     AIGLLVDDAIVVVENVERIMSEEGLSPREATAKSMEQITSALIGIGLVLSAVFGPMAF
                     FPGSTGIIYRQFSITIISAMLLSVVVALILTPVLCASLLKPVQAGHEPAENANPLFRP
                     FFLWFDRMFFGLRKRYVDLVGHSLTRKKRYLVVFLVIVVAMGFLYLRMPTAYLPDEDQ
                     GLLLAQVIMPKGSTMEQTKEVTKKIARYFDENEKEAVESCMIISGIGFSGRAQNNGMV
                     FIKLKDWTLRNRADLKVKAVAGRAMMAFSGIREAMVFAFPPPAVVELGIAKGFDFELL
                     DRGGLGHQGLIEAQYQLLGMAAQDPRITAVRPNGMEDVSEYRVDVDWEKAGALGVPIS
                     SIHNTVSAAFGSAYVNDFVKGGSVKRVFVQADAPYRMLPKDLDKLYVRNTSGLMVPFS
                     SFASGRWASGSPMLERFNGFPSLNIWGEPAPGKSSGEAMKAMEEIVEKLPKGIGYDWA
                     RLSYQERMSSSQAPLLYTFSIFVIFLCLAALYESWTIPISILLVLPLGVIGGIIATSM
                     RGLANDVYFQIGLLTTLGLTTKNAILIVQFAKGGLERGMSLIDATVEGAKLRFRPIVM
                     TSLAFGFGVLPLAISTGAGAGAQNAIGTSVLGGMFSATILIVIFAPLFFVLTEKLFGR
                     KHKQTSGGAGKKIFSNSQPAKSTATDNANPPEVK"
BASE COUNT         1295 a         1377 c         1455 g         1334 t
ORIGIN      
        1 aatggcgtct ttcggcgcat agatgtcgtt atttgtcatt aatatatatg gcgccctgct
       61 ttgatgaact ccccggttga tggatttcgc gagaccttgg ttaatctcgt ttcttatgag
      121 tattatgttt aaggcttttt ctctcgcggt tttttcaacc atggctttta attcatgctg
      181 ctttggcgat gcgtcgtcga tgatgataat ttcaaaatca tttttcatgg ggctcatcgc
      241 caggctttcc agaaaaagtt tttccgtcag atcaattaaa tattgattgg gacaatagac
      301 gggcgtgatg atgcttagga tataatcgtt tctgtccata gggttcattc ttttactggt
      361 gttgtcgatt ggaacgaatg aaacataaag aaggctttcc ggtatgtcaa gacgatatta
      421 tctccgcagg aatattttta cggacaaaga cgaatgcggg aagaatgcac cgcattgaca
      481 ttaaaaggtg aaagaaaggg ccaacgctcc gaaatgctga gcatcctttt gcttttcaaa
      541 ctctttggtc cggtgaacgt aagagtaggt gattttgatc cggtgaatga tgatgcccag
      601 gcctccgatg atctcggcga cgaaaggttc tttcttgacg ctgtggctgt cgcggaacgt
      661 gtttccgtca agcaggatat tgcgggcgac ggcttttccg tcgaccgcaa aaaaagcatg
      721 caccccgaaa cggcgcaggg gcgcgaagaa acgcggatcc gtatcgtcca ggggggcgct
      781 gctgtcggag cccggacgga tcagatacgt tccaaaatca ttcgggacat tccatccgaa
      841 gcgtacctga ccgccgagat tggcgccggt cagggcattt cctatggcca gtccggtgtg
      901 cggaatcaaa tccaggccca aaccggatga attccttaat tgcagcaatc tccatttccg
      961 ttcgagatat acattgagaa tcggttcgtc gcgtagttga tttttccagc ccctggcgac
     1021 ttcggaatcc gtccattcat gaattttcgt ctggatatct tccgcgtagg aatgacgtcc
     1081 gacgatgccg atgcccagct ccagcgtgtc catgtgcttt gtatttttac tgtgcagacc
     1141 gagtcccaga tacgtgatgc cggcgtaggg acggtcattc ttgagcaatt tccttttttc
     1201 tttatgttcc ggcgtgtaaa tgttttgtcc gaaagataaa gaaacagaac gctgttgatc
     1261 aggatcgttg accagaggta ccttttcgat gatgctttgc atccagtgcg ggatggatgt
     1321 gatctcccgg tagtttgaaa gatcccggga aatccaggag aattttgttc cgtgggtgta
     1381 ataacggtcc agatgctcaa aaagatcatt ttccatatac gccgtcagcg tctccgacga
     1441 tcgtgcctga ttgttcgaag cgatagacga ttgcgccgcg gcggcgaccg tgagcattcc
     1501 aataacggcc atccgtagac tttttttcat gggggaaaat ctgtctttaa ttatcttggc
     1561 ctccacccag ggctttatag agcgtgatga gattgttgat tccggctaaa cgaagcatga
     1621 tcagcccctg ctgcgctccg taaagagagc gttgcgcgtc gagcacggag aggtagctgt
     1681 caattccctt attataacgc gcttcagata attggtacgt taccgccata gcgtcaacca
     1741 gcgatagttg agccgccagc cgctggttga cagttcctct ttcagccaga gcatcggcca
     1801 cttcccggaa agccgtctgg atggcctttt catactgggc tatggatatt tctttttcaa
     1861 ctttggtgac ctcataggca gaccaggtac gggcgtcgaa tatcggtacg acaatctgag
     1921 gtgagaacag ccaggtgcct gatccggatt tgaaaagacc ggataactca tcactggctg
     1981 ttccagcgga agccgtcagc gtgatgcggg ggaaaaaagc cgcgcgggcc gccccgatat
     2041 tggcattggt ccctttgaga cgatgttccg ccgccagaat gtccggccgt gacaaaagcg
     2101 ctgcggatga cagaccggcg gaaatgtccc gggggggcgt gacgtttttc aaatccgcgg
     2161 gcagaagctc tggggaaaca ggcgaaccga caagaagatt cagggcattt tcgtcctgag
     2221 cgaccagttg tgtataacgt gcaaaatcgc ctctggcact atccacctgg ctttgtgccc
     2281 ggcggagatc aagctccgag gccatgccga cgtcagccct tttcttgatc aagcgataaa
     2341 aatcctcctg cgtcttcagc gttgatgtta cgagctggaa agcttctttg tccgcggcca
     2401 gcgccatata gacttgagca atcgatgata caagcatgat ttgcgcgctg cggcgagcct
     2461 cttcagtggc gaggtattgt tccaatgccg cgtcttttaa attgcggata cggccgaaaa
     2521 aatcaatttc ccaagaagca atgccgagat tgacgtcgta tctttcggcg gtatatgcgc
     2581 tgccggttga tgacagatcg gcgggaattc tttctttgta catgctgcct gcggcattga
     2641 ccgaaggaag aagctcggcg cgttgaatgc cgtagtaagc tcttgctctt tcaacgttga
     2701 gagcggctat ccggagatcg cggttgttat tcagcgccgc ttcgatgacc tttcgtaaat
     2761 tttcatccgg aataaattcc ctccagggca actccattgc cgccggcacg gatggacttg
     2821 gttttgtttc ctcgtaggcc gggccggtgg gccattccga aggcaccggg gcatccgggc
     2881 gcgaatattg aggggccatc gtgcatccgt ttgcaaggaa aaacatgaca acaagtaaaa
     2941 ggattatttt tttgttcatc ttatttaacc tccggcggat ttgcattatc cgtcgccgtt
     3001 gattttgctg gttgtgaatt ggaaaatatt tttttgcctg caccgcctga tgtttgttta
     3061 tgtttgcggc cgaataattt ttcagtcagg acaaagaaca acggcgcaaa aatgacaatc
     3121 aggatggtgg cgctgaacat gccgcccaac acactggtgc cgatggcgtt ttgcgcgccg
     3181 gcgccggcgc cggttgaaat agccaggggc agcactccga atccgaaggc cagagacgtc
     3241 atgacgatag ggcgaaagcg cagctttgct ccttcgacgg tggcgtcgat cagactcata
     3301 cctcgttcca gtccgccttt ggcaaactgg acaatcagga tggcattctt ggtggtcaaa
     3361 ccgagcgtgg taagcaaccc gatctgaaag tacacatcat tagccaaacc gcgcatactg
     3421 gtggcgatga ttcccccgat gactcccagc ggcagcacca gcaggatgga aatgggaatc
     3481 gtccagcttt catagagcgc cgccagacac agaaagatta cgaaaattga aaaggtgtaa
     3541 agcagagggg cctgcgagct cgacatgcgt tcctgatagg aaagtctggc ccagtcgtac
     3601 ccgatgcctt tgggcagttt ttcgacaatt tcttccatgg ctttcatggc ttctccggaa
     3661 ctttttcccg gcgccggttc gccccagata ttcagggagg gaaagccgtt gaagcgttcc
     3721 agcatcgggg atccggatgc ccagcggccg gaggcaaagg aggaaaaggg caccatcagg
     3781 ccggacgtgt tgcggacata gagcttatcc aggtctttgg gcagcatacg gtagggggcg
     3841 tccgcctgaa caaacacccg cttgacggat ccgcctttga cgaagtcgtt gacataagcg
     3901 ctgccgaaag ctgcggaaac ggtattgtga atggagctta tcggaacgcc gagggcgccg
     3961 gccttttccc agtccacgtc cactctgtat tcggatacat cttccattcc gttaggccgg
     4021 acagccgtaa ttctggggtc ttgcgccgcc atgcccagaa gctgatactg ggcctcaatc
     4081 aaaccctgat gtccgagacc gccccgatcc agaagttcga aatcgaaccc tttggcgatg
     4141 cccagttcga caacagcggg aggaggaaaa gcgaaaacca tcgcttcccg gatgcctgaa
     4201 aaagccatca tggccctccc tgcaacggct ttgactttga gatcagcccg gtttctcaat
     4261 gtccagtcct tcagcttgat aaataccatg ccgttatttt gcgcccggcc ggaaaaaccg
     4321 atgccggaaa tgatcataca ggattccacg gcttcttttt cattttcgtc gaaataacga
     4381 gcgatttttt ttgtcacttc ttttgtctgt tccatggtgg aacctttagg cataatgacc
     4441 tgcgccagca gcaatccctg atcttcgtcc ggcagataag cggtgggcat gcgcagatac
     4501 aaaaacccca tggccacgac gatgaccaga aagacaacca gatagcgttt ttttctggtc
     4561 agagaatgac cgaccagatc cacgtaacgt tttctgaggc cgaagaacat gcggtcaaac
     4621 cacaggaaaa agggacgaaa aagaggattg gcattttccg ccggctcatg tcctgcctga
     4681 accggcttca gcagcgatgc gcaaagcacg ggcgttaaaa tcaacgctac caccaccgac
     4741 agaagcatag cggaaataat cgttatggag aactggcgat agatgatgcc ggtggaaccg
     4801 ggaaaaaatg ccataggacc gaaaaccgct gagagcacca gtccgatgcc gattaaagcg
     4861 ctggtgatct gttccatcga tttggccgtg gcttcgcggg gagacaggcc ctcctcactc
     4921 ataatccgtt caacattttc caccacgaca atggcatcgt ccaccagaag tccgatcgcc
     4981 agcaccatgg caaacatcgt gagcatatta atggaatacc cgaacagtcc cagaatagcg
     5041 aacgttccca gaagaaccac cggcacggca atggtcggaa tcagcgtggc ccggatgttg
     5101 cccagaaaaa gcagcatgac cagaaagacc agcagaatcg cttcgaagag cgttttaacc
     5161 acttcattga tcgctactct tatgaaaggc gtggtgtcat aaggatagac aaccttcagt
     5221 cctccgggga agaatttgct caactcggca agttttgcct tgaccctgtc cgccgtatcg
     5281 agggcgttcg cgcccgcggc ctgacggatt gccagaccgg ttgaaggctt gccgttatgt
     5341 tcaacctgga tgtcgtaaat ttccgtccct aattccgtcc gtccgatatc gcgcatccga
     5401 atgatagacc catccgggtt ggtgcggatg gggatggcgc cgaattcttc gggggttctt
     5461 a
//
LOCUS       DAOOLB010000119         5385 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_18291_length_5385_cov_46.489306, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000119 DAOOLB010000000
VERSION     DAOOLB010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18291_length_5385_cov_46.489306"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2643)
                     /gene="fdhF"
                     /locus_tag="PLF77_10235"
     CDS             complement(<1..2643)
                     /gene="fdhF"
                     /locus_tag="PLF77_10235"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015947363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1573..1575),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HPC09275.1"
                     /translation="MEKKIPIRIDGKDVEATAGQTILECARDNGIFIPTLCHYTKTTN
                     VGACRVCVVEVEGARSLAASCCMPVTPNMVVRTDTEAVRKAQKMIIELLWSSGDHNCL
                     TCEQNGNCELQNLVYWLQIDKPRFEIQVPGYAKDDSNTMIARDMNKCILCGRCVRACN
                     EIQVNEVLDFANRSYKAKVGPAFDQDYIDSTCVFCGECVQACPTGALTFKQAKFAGRP
                     WELAKVKTTCTYCGVGCQMDLYTKDNKIVKVMGNREYGQPNDGSLCVKGRFGMDFLSH
                     PDRLKTPLIRYKKNENFKEATWDEAFTFIADKLSRIKKEHGADSIAGLASARCTNEEN
                     YLFQKFLRAGIGTNNVDHCARLUHSVTVAGLAATFGSGAMTNSIDEIEFTDLILATGT
                     NTTENHPVIGAKVKRAVRRGAKLIVIDPREIDLVKYADIWLRQNPGTDVAVINGMMNV
                     IISEGLYAKEYVKDRTEGFDALKKMVEKYTPEKVEKISGVPAEDLKKAARMYAKADRA
                     SILYAMGITQHITGTDNVKSCANLSMLCGNVGIEGGGVNPLRGQNNVQGACDMGALPN
                     VYPAYQQVANEDVRKKFEGFWNAKLSPKPGLTIMEIMAAAGKGDIKAIYIMGENPMLS
                     DPDLHHVKKELQKLDLMIVQDLFLTETAELADVVLPVASFAEKDGTFSNTERRVQRVR
                     KALDAPGEAKADWEVICGIANKMGYEMNYASAREIFEEVAKVTPSYAGISYERLEKGG
                     IQWPCPTPEHQGTKFLHKDKFSRGLGLFTAIEYIPPDELPDKQYPFLLSTGRVLYHYH
                     TGTMTTRTKGLTERYPESLVEINPADAEKMGIAEGQKVKVTSRRGTVEAKATITKKSA
                     PGSIFMNFHFADASVNLLTNPALDP"
     gene            3184..4350
                     /locus_tag="PLF77_10240"
     CDS             3184..4350
                     /locus_tag="PLF77_10240"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPC09276.1"
                     /translation="MAEISVNGFCDPAFDKVKQAFIGNFKKGKERGAAVSIVIEGKTV
                     VDIWAGYADFWKRRPWQKDTLVNIYSTTKGVTAICAHRLIEQGRLDPDKPVSAYWPGF
                     EKNGKEAITVRMLLNHTAGMVAIKKRQPGKALYDWDAMVNALENQSPWWKPGKLGYHA
                     ITFGWLVGQVIRNITGMSVGQFLKKEITGPLDLDIHIGLDEAEHHRSANMVMVRIPTI
                     HPDCLKLTRAIVLNPFGATSMALGNPFSIATGVNSRSWRSAEIPSANGQAAARALAKL
                     YGILANGGKAGNIQVLSPGEIDRCSEETSGGDDAVLKLKTRFSLGFMLNQDNPSGNMG
                     PGKKTFGHPGAGGSLAFADPDAHLGFGYVMNKMDTFILVDPRAKRLIKAVYACL"
     gene            complement(4538..>5385)
                     /locus_tag="PLF77_10245"
     CDS             complement(4538..>5385)
                     /locus_tag="PLF77_10245"
                     /inference="COORDINATES: protein motif:HMM:NF016131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HPC09277.1"
                     /translation="IIDRALEQNPENKEIIKAFAPIIIRQRQLAEKPSEQKLDCLAID
                     KQKLKAGVPVSRQINLILPGDPLKEIALSLAEAVKEGMPQLTENIDLIAGLIKDDKIK
                     PADYFKSTPDGESMATDVWAKDLKVSPSNASFLMSLVSRVILEQRANEITAAIGAFDW
                     EKGYCPICGEFPSIALIEEEGGKRFLHCSACGYYWRFTRVSCPYCEKETRQGMEYFYV
                     ENKKQESAFVCDQCKKYLVTLYRAGQVFARDMDVSAISLIHMDLLMQNKGYEPMTACT
                     WNVLS"
BASE COUNT         1222 a         1355 c         1430 g         1378 t
ORIGIN      
        1 cgggtccaga gccggattgg tcaacaggtt gacggatgcg tccgcgaaat ggaagttcat
       61 gaagatcgaa ccgggagcac tctttttggt aatggttgcc ttggcttcaa ccgtaccgcg
      121 tcttgaggtc accttgactt tctggccttc ggcaataccc attttttcgg catcggccgg
      181 gttgatttcc accaggcttt cgggataacg ctcggtcaga ccttttgtac gcgtggtcat
      241 ggtaccggtg tggtaatgat acaggacgcg tccggtggaa agcaggaacg gatattgttt
      301 gtccggcaat tcatcgggcg gaatgtattc aatggcggta aacaatccca gaccgcggga
      361 gaacttatcc ttgtgcagga atttggtccc ctgatgttcc ggcgtgggac agggccactg
      421 aatgccgccc ttttccaggc gctcgtaaga gatgcccgca tagctggggg tgactttcgc
      481 cacttcttca aaaatttccc tggcggaagc gtaattcatc tcgtaaccca tcttgttggc
      541 aatgccgcag atgacttccc agtcggcttt ggcttcaccc ggcgcgtcga gcgctttgcg
      601 cacgcgctgt acgcggcgtt cggtattgga aaacgttcca tccttttcgg caaagctggc
      661 caccggcaaa accacatcgg ccagttcggc ggtttcggtc aggaacagat cctgaacaat
      721 catcaaatcc agtttttgaa gttctttttt cacgtggtgc aaatcggggt ctgaaagcat
      781 ggggttttca cccatgatgt aaatcgcttt gatatcgcct ttgccggcgg ctgccatgat
      841 ttccatgatg gtcaggcccg gtttggggga aagcttggcg ttccagaagc cttcaaactt
      901 tttacggaca tcttcattgg ccacctgctg gtaggcggga taaacattgg gaagtgctcc
      961 catgtcgcag gcgccctgaa cattgttctg acctctgagc ggattcacgc cgccgccttc
     1021 aataccgaca ttgccgcaca acatggaaag attagcacag ctcttgacgt tgtcggttcc
     1081 ggtaatgtgc tgggtaatgc ccatggcgta aaggatgctg gcacggtctg ccttggcata
     1141 catccgggcg gctttcttca aatcttccgc gggcacgccg gaaatctttt cgaccttttc
     1201 cggtgtgtat ttttcgacca tcttcttgag cgcgtcgaat ccttcggttc tgtctttcac
     1261 gtattccttg gcataaagac cttcggagat gatgacgttc atcattccgt tgataacggc
     1321 aacatcggtg ccgggattct ggcgcagcca gatatcggcg tatttgacca gatcgatttc
     1381 gcggggatca atcacaatga gtttcgcgcc gcgccggaca gcgcgtttga ccttggcgcc
     1441 gatcaccgga tgattttcgg tggtgttggt gcccgttgcc agaatcaggt ctgtgaattc
     1501 aatttcgtct atggagtttg tcattgcacc gctgccgaat gtggcggcca gaccggccac
     1561 cgtgacagag tgtcagagac gggcgcagtg atcaacattg ttggtgccga ttcccgcccg
     1621 caggaatttc tggaacaggt aattttcttc attggtgcag cgcgccgagg cgagtcccgc
     1681 aatgctgtcg gcgccgtgtt cttttttgat cctggaaagt ttgtccgcga tgaaggtaaa
     1741 ggcttcatcc caggtcgctt ctttgaagtt ttcattcttt ttataacgga tgagcggtgt
     1801 tttcaggcga tcgggatggg aaagaaaatc catgccgaaa cgtcccttga cgcacaggct
     1861 gccgtcattg ggctggccgt attcgcggtt gcccatgacc ttgacgattt tattatcctt
     1921 ggtgtagaga tccatctgac aaccaacgcc gcaataggtg caggtggttt tgacttttgc
     1981 aagttcccac ggacggccgg cgaatttcgc ctgtttgaat gtgagagcgc ctgtgggaca
     2041 ggcctgcacg cactcgccgc agaagacgca ggttgaatca atgtaatcct ggtcgaatgc
     2101 ggggccgacc ttggccttat aactgcggtt ggcaaaatcc agaacttcgt tgacctggat
     2161 ttcattgcag gcgcgcacgc agcgtccgca gagaatgcat ttgttcatgt cgcgcgcaat
     2221 catggtattg ctgtcatcct tcgcataacc cggcacttga atttcaaagc gcggcttgtc
     2281 aatctgcagc cagtaaacca gattctgcaa ttcgcaatta ccgttctgct cgcaggtgag
     2341 gcagttatga tctccggacg accagagcag ttcgatgatc attttctggg ccttgcgaac
     2401 ggcttccgta tcggtgcgga caaccatatt gggcgtcacc ggcatacagc aggatgcggc
     2461 aagagatcgg gcgccttcca cttcgaccac gcatacgcgg caggcaccga cattggttgt
     2521 tttggtataa tgacaaaggg tcggaataaa aatgccgtta tcccgtgcgc attctaaaat
     2581 tgtctgaccg gcagtcgctt caacatcttt tccgtcaatt ctgataggta tttttttctc
     2641 catgtctttc tccaatctta aattatctga actcttactg accaagagtt atattttttt
     2701 taacatcgat ttcataacga caagcaatcg ggttgtcaat tctgtcaata ttgacttatg
     2761 ccttttttca cataggccaa aaatgacaat acgttaaata tatcaaatac ataatgcaat
     2821 gccgctaccg acgtccgccg taaataaatt gggcgcaacg ggtggaaatg cccgaagcct
     2881 gactggactt gtcagacatg gtccggtgag gatgggacgt gatgtttctt ttgcctatgt
     2941 tgttgcaaca gcaaaagcgt tgcctgtgca aaatgatgca agggttttat cgtcaggagg
     3001 agtatcgtta caggccatca tagtcgttga caggcaatca ggcattgatg tagcttgcaa
     3061 aagcgtgtag ctctgaaaaa gggcgtgcat caggtcagtg cacggcaaca cacttatgcg
     3121 gttgatttgt tctgcaaaaa cgaatcagac gacgaatgat cgaatgtaat accgggagat
     3181 aatatggctg aaataagtgt caatggtttt tgtgatccgg cttttgataa ggtgaagcag
     3241 gcgtttattg gcaattttaa aaaaggcaag gagcggggag ctgccgtttc aattgtcatc
     3301 gaaggcaaaa cggttgttga catctgggcc ggttatgcgg atttctggaa gaggcggcca
     3361 tggcaaaaag atacgctggt caacatctat tcgacgacca agggcgttac cgccatttgc
     3421 gcccaccggc tgatcgagca gggacggctg gatccggata agccggtgtc ggcgtactgg
     3481 ccgggatttg agaaaaacgg aaaagaagcc attacggttc gcatgctgct gaaccatacc
     3541 gccggcatgg tcgctataaa aaaacgccaa ccgggaaagg cgctgtacga ctgggatgcc
     3601 atggtcaacg ctctggagaa ccagtctccc tggtggaaac ctggcaagct cggctaccac
     3661 gccattactt tcggctggct ggtggggcag gtcattcgaa atattaccgg catgtccgtg
     3721 ggacaatttt taaaaaagga aattaccggt ccgctggacc tcgatattca tatcggtctg
     3781 gatgaagccg agcatcaccg gtcggcaaac atggtcatgg tgcggatccc cacgatccac
     3841 ccggactgtc tgaaattaac cagggcgatt gtcctgaatc cttttggcgc aacatccatg
     3901 gccctgggca atcccttcag tatcgccacg ggcgtcaaca gccgttcctg gcgaagcgcc
     3961 gagattcctt ccgcaaacgg ccaggccgcg gcccgggccc tggcaaagct atacggtatt
     4021 cttgccaacg gcggcaaagc gggcaacata caggtcctgt cacccggtga aatagaccgg
     4081 tgctccgagg agacctccgg cggcgacgat gccgttttga aattgaaaac gcgattcagc
     4141 ctgggcttca tgctgaatca ggataacccc tcgggcaaca tgggccccgg caaaaagaca
     4201 ttcggccatc ccggcgcggg cggctccctt gcctttgccg atccggatgc gcatctggga
     4261 ttcggctatg tgatgaacaa aatggatacg tttattctgg tcgatccccg ggcaaagcgt
     4321 cttatcaaag cggtgtacgc gtgcctgtga cggtttcatt gtttcaatca gaaagctgat
     4381 taccgcaagt cccctgcaac ggcatcagtt ggtgatcatc agaaatattg tatcgacaac
     4441 tgccggaatg cgaaaaaaca attcttcaga aagaagcaaa tacaggacgt gatgcaagct
     4501 gccgggtcaa tttctgtttc gggcgatggt caattgatca gctcaaaaca ttccaggtac
     4561 aggcggtcat gggttcgtag cctttgttct gcatgagcag atccatgtga atcaggctga
     4621 tggcggaaac atccatatcg cgcgcaaaga cctggcccgc ccggtaaagg gtgacaaggt
     4681 attttttaca ttggtcacag acgaaagcgg attcctgttt tttattttcc acatagaaat
     4741 attccatccc ctgccgggtt tccttttcac agtaaggaca actgacccgc gtaaagcgcc
     4801 agtaataacc gcaggcagag caatgcagga agcgctttcc gccctcttcc tcaattaacg
     4861 caatggacgg aaattctccg caaatcgggc agtacccttt ttcccagtca aaggcgccga
     4921 tggcggccgt tatctcgttg gctctctgtt ccaggataac gcggctgacc aggctcatta
     4981 aaaatgacgc attggacggc gagactttta aatctttcgc ccagacatcg gtggccatgc
     5041 tttccccgtc cggggtgctt ttgaaataat ccgccggttt gattttatcg tctttgatca
     5101 gccctgctat cagatcgata ttttccgtca gttgcggcat gccttccttc accgcctcgg
     5161 ccagcgacag agcgatttct ttcaacggat cgcccggcaa tattaaattg atctgacggc
     5221 tgaccggcac gccggccttg agtttctgtt tatcgattgc caagcagtcc agtttctgct
     5281 ctgaaggctt ttcggcaagc tgtctctgcc ggatgataat cggtgcaaac gctttaatga
     5341 tttccttatt ctcaggattc tgttccagag cgcggtcaat gatat
//
LOCUS       DAOOLB010000120         5380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_18306_length_5380_cov_53.453521, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000120 DAOOLB010000000
VERSION     DAOOLB010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5380
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18306_length_5380_cov_53.453521"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /locus_tag="PLF77_10250"
     CDS             complement(<1..659)
                     /locus_tag="PLF77_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPC09278.1"
                     /translation="MTLIETRQLVKTYDVGDEGVHALENVSVKIDKGEFVAIMGPSGS
                     GKSTFMNIIGCLDQPTAGDYFLDGQTISGLSRDDLAAIRNQKIGFVFQGFNLLSRTSA
                     LENVELPLLYNHVPARQRKEESLKALKMLGLEERAYHHPNQLSGGQQQRVAIARALVN
                     QAPILMADEPTGNLDTKTSIEIMELLVQLNRDSGTTIILVTHEPDIADFSKRIIRFVD
                     G"
     gene            complement(659..1843)
                     /locus_tag="PLF77_10255"
     CDS             complement(659..1843)
                     /locus_tag="PLF77_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPC09279.1"
                     /translation="MKKKIIVISVLLIAAFAAGIFFYVNRDGKKAVYKTEKISRGEVK
                     SVVTATGTVNAVTTVSVGTQVSGTIRTLFADFNSPVKKGQLLAQIDPATFRAQVDQAR
                     ANLLSAQANLEKAGVAVVDARRTYERNKELFSQNFIARSDLDTAETALHAAQAQQKVS
                     EAQVAQARASLRMAETNLQYTNIISPVNGTVISRSVDVGQTVAASFQTPTLFSIAQDL
                     TQMQINASVDEADIGQIRAGQDVSFTVDAYPDTDFSGKVSEVRNAPTTVSNVVTYDVI
                     IKVDNPELKLKPGMTANVSITIETRPDVLRVPNAALRFKPAEVGEKGVSAKELQDIKG
                     TKVWVLENNQPKPVGVTLGLSDGNFSEIVKGDLRTGQEIVTDSLNKKGSSTTQRPPPR
                     FM"
     gene            complement(1840..3117)
                     /locus_tag="PLF77_10260"
     CDS             complement(1840..3117)
                     /locus_tag="PLF77_10260"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPC09280.1"
                     /translation="MQKKIMLLFLIVLIPFAAAASEPAGGTASYDLDQCIAVALDKHP
                     SLKASAGDIRARESRVGQARSKYYPWLDVSAGYQKIGPTTPPGDSIDSYDQYSTGINL
                     GVTLFDFGKTSANVAIQDLNVNASRADYDDVKAQIILNVKKAYYTLLQNQRNRDVAVD
                     TMQQFQQHLDQANAFFRVGTKPKFDVTKAEVDLGNARLNVLKAENAVRAARITLKDVM
                     GIPENADFTIVDNLSFQKQAWILDDILNKAYAVRPDLLSIIARREAAERSIDLARKDY
                     LPTLSGSAGYGFSGRDFPLGRGWNAGASLSFPLFSGFSTNYQIDEAEANLAILTANEE
                     TVRQAIHLDVQQAYLNVQDTAEQIAMAEMTVVQAQENYDLASGRYRSGVGSPIEVADA
                     TIMLNNARAGLNTALCNYRIAQAVLEKSIGAKP"
     gene            complement(3135..3770)
                     /locus_tag="PLF77_10265"
     CDS             complement(3135..3770)
                     /locus_tag="PLF77_10265"
                     /inference="COORDINATES: protein motif:HMM:NF020771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CerR family C-terminal domain-containing
                     protein"
                     /protein_id="HPC09281.1"
                     /translation="MVLASGKTNSKTRTAIIEAAGKIFAEEGYLKATVRDICSSAGAN
                     LAAVNYYFGDKKGLYLSVLKYYQEMAFQAYPPDLGIRASQKPEEKLKIYIRMFLKRIM
                     DDGRPVWFGRLLAREFSEPTCALDVLVNETIRPSFQLLTEIVADVLGKTPREKMVRLC
                     SMSIVGQCLYFRHSQPIMTRIYPEATFGAEQIDEITEHIASFSIGGLRKAQ"
     gene            3984..4976
                     /locus_tag="PLF77_10270"
     CDS             3984..4976
                     /locus_tag="PLF77_10270"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HPC09282.1"
                     /translation="MNTKNMLRYISALTVIAAAVYFFYLQFKKNADAIGAWKFSINPL
                     EMSLAVIFGSCALLVGPLVWRLFVNSYLKKKLNYTESFVLYCTSAMFKYIPGKIWTYA
                     AQIALLSSKGISKVALLYINLVSFICLLFVAVPFFLYYYFIYVQEMSLGATLLILTLF
                     MLMDVVFIIANHTIVNYLIRPINRLFKLNIKPIRTDKIIFVYVQMLYSLACVLLGIAL
                     YFLARGVNMPLPFSQIIAMMATICVSLILGLLAFFTVGGLGVREGAMFFMLKQFSNIE
                     AALILPVISRFLITLVELLMAVTAIIIGLKYGYFHDIEKVKPAADSALPRESAG"
     gene            5013..>5380
                     /locus_tag="PLF77_10275"
     CDS             5013..>5380
                     /locus_tag="PLF77_10275"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPC09283.1"
                     /translation="MMTFTDLLKNRRSIRDFQDKNVPMNLIEEILQESTLAPSASNNQ
                     PCRFSIVHCRKTIKALSDESKANLLEDYAGNKKSLLPQYIDMLKDENFNVFYNAPCVI
                     FIIGSKSVKSLDVDCALAAS"
BASE COUNT         1249 a         1429 c         1289 g         1413 t
ORIGIN      
        1 tgtccgtcaa caaaccggat gatgcgttta ctgaagtcgg cgatatccgg ctcatgtgta
       61 accagaataa tggttgtgcc tgaatcccgg ttcaactgaa ccagaagttc catgatttca
      121 atgctggttt tcgtatccag attgcccgtc ggttcatcgg ccattaatat aggcgcctga
      181 ttcacaagcg cgcgggcaat ggcaacgcgc tgttgctgtc ctcctgacag ttgattggga
      241 tggtgatacg cccgttcctc cagacccagc atttttaacg cttttaagga ttcctctttg
      301 cgttgccttg ccgggacgtg attataaagc aaaggcaact cgacgttttc cagcgccgac
      361 gttctggaca gcaaattaaa tccctgaaag acaaaaccga ttttctgatt gcggatcgcg
      421 gccagatcgt cccgtgacag accgcttatc gtttgtccgt ccagaaaata atcccccgca
      481 gtcggctgat ccagacaccc gatgatattc ataaaggtgg atttgcccga accggacggc
      541 cccatgatgg cgacaaattc acctttgtcg attttgacgg agacgttttc cagcgcgtga
      601 acgccttcat cgcccacatc atacgttttg acaagctgcc ttgtttcgat cagagtcatc
      661 acataaacct cggaggcggg cgctgtgtgg tgctgctgcc ttttttattc aggctgtccg
      721 tgacaatctc ctggcccgtg cgcaggtcgc ctttcacaat ttcagaaaaa ttcccgtcgc
      781 tcaatccgag cgtaacgccg acgggtttgg gctgattgtt ttccagaacc cagactttcg
      841 tccctttgat gtcctgcagc tctttcgctg atacgccttt ttccccgacc tccgcgggtt
      901 taaaacgaag agccgcattc ggaacccgca ggacatccgg acgcgtttct atggtgatgg
      961 aaacattggc cgtcatcccc ggctttaatt ttaactcggg attatccacc ttgataatga
     1021 cgtcgtacgt caccacgttg gagacggtgg tcggcgcatt gcgcacctcg gaaaccttgc
     1081 cggaaaagtc cgtgtccgga taggcatcca ccgtaaacga gacgtcctgt cccgcccgga
     1141 tctgaccgat atccgcttca tccacactgg cgttaatctg catttgcgtg agatcctggg
     1201 caatgctgaa aagcgtgggc gtctggaaac tggcggccac cgtctggccg acatctacgc
     1261 tgcgggaaat caccgtgccg ttcaccggag aaataatatt ggtgtactgt aaattggttt
     1321 ccgccattct cagagaggcc cgcgcctgcg caacttgcgc ctcgctgact ttctgctggg
     1381 cttgtgcggc atgcaaagcc gtttccgccg tatcgagatc gctgcgtgcg ataaagttct
     1441 gggaaaaaag ttccttgttt cgttcgtagg ttcgccgggc gtcaacgacg gcaacccccg
     1501 ctttctccag attggcctgg gccgaaagca ggtttgctct ggcctgatcc acctgcgccc
     1561 ggaacgtggc cggatcaatc tgtgcgagaa gttgcccctt tttcaccggc gaattgaaat
     1621 ccgcaaaaag agttctgatg gttcccgata cctgcgtgcc gacagaaaca gtcgtgacgg
     1681 cattcacggt gccggtagcg gtcacaacgg atttgacttc accgcgcgaa attttttccg
     1741 ttttatagac ggcttttttc ccgtctctgt tgacataaaa gaatatcccg gccgcaaagg
     1801 cggcaatcag caatacggag atgacaatga ttttcttctt catggttttg ctcctatgga
     1861 tttttccaga acggcctgtg cgatcctgta attacaaagc gccgtattta aaccggctct
     1921 cgcgttattg agcataatcg tcgcgtcggc aacttcaatg ggactgccga cgccgctccg
     1981 ataacgcccc gacgccagat cgtaattttc ctgagcctga acgaccgtca tttcagccat
     2041 ggcaatttgt tcagcggtat cctgaacatt cagataggcc tgctgaacat ccaagtgaat
     2101 ggcctggcgg acggtctctt cgttggctgt cagtattgcc agattggctt cggcttcgtc
     2161 aatctgatag ttggtcgaaa accccgaaaa aagcggaaaa ctgagagacg cgcccgcgtt
     2221 ccagccgcgg cccagcggaa aatcacggcc ggaaaagccg tatccggcgc tccccgacag
     2281 ggtcggaaga taatcttttc tcgccagatc gatcgaccgc tcggctgcct cgcgtctggc
     2341 tataatcgaa agaagatcag gacggacggc atacgccttg ttcaaaatat cgtccaggat
     2401 ccatgcctgt ttctgaaaag acaggttatc gacaattgta aaatcggcgt tttccggaat
     2461 gcccatgacg tccttcaacg tgatccgtgc agcacgaacg gcattttccg ccttcaaaac
     2521 attcagccgc gcattgccta aatcgacttc cgctttggtc acatcgaatt tcggtttggt
     2581 gccaacgcgg aaaaaagcgt ttgcctgatc cagatgctgc tggaactgct gcatcgtatc
     2641 gacggccaca tcccgattgc gctggttctg caaaagcgtg taataagctt ttttcacatt
     2701 cagaatgatt tgcgctttga catcgtcata atcggcgcgc gacgcgttta cgtttaaatc
     2761 ctgaatggcg acatttgctg acgtcttgcc gaaatcgaaa agggtcacgc ccagattgat
     2821 ccccgtcgaa tactgatcgt acgaatcaat ggaatcgccg ggtggcgtgg tcggaccgat
     2881 cttctgatag ccggcggaca catccagcca cggatagtat ttggacctgg cctgcccgac
     2941 ccggctttcc ctggcccgga tgtcgccggc ggacgccttg aggctgggat gcttgtccag
     3001 agcaacggca atgcactgat caagatcata cgaggccgtt ccacctgccg gctccgaagc
     3061 ggcggcggca aacggaatca agacgattaa aaaaaggagc attatctttt tttgcatttt
     3121 ttcaccctgg ctgattattg cgctttcctc aggcctccta tggaaaagga ggcgatgtgt
     3181 tcggttattt catcgatctg ttccgccccg aaggttgctt ccggataaat tctggtcatg
     3241 atcggctgcg aatggcggaa ataaagacat tgaccgacaa tactcattga acacagccgc
     3301 accatttttt cccggggcgt tttgcccagc acgtcggcga cgatttccgt aaggagttgg
     3361 aaagaaggac ggatggtctc gttgaccaga acatccagcg cgcaggtcgg ttcgctgaat
     3421 tcccgcgcca ggagcctgcc gaaccagacg ggacgtccgt catccataat ccgtttcaga
     3481 aacatccgga tataaatttt aagcttttcc tccggtttct gagaggcccg gatacccaga
     3541 tcgggcggat acgcctgaaa agccatctcc tgataatact tcaacacgga cagatacaat
     3601 cccttcttgt ccccgaaata ataattgacc gccgccagat tggccccggc gctgctgcaa
     3661 atatcccgga cggtcgcctt gaggtaacct tcttcggcaa agattttccc tgccgcctca
     3721 atgatcgcgg tgcgtgtttt tgaatttgtt ttcccggagg ctaaaaccat ttttcaaaca
     3781 cctgttttaa acaagcgtat gaatattgaa ccgctttgtc aaggtttttt tcacaaaaac
     3841 gatcaacgtc ccgtttttcg gggcattttt tgacaacagc gcctatttgt catatacagt
     3901 taaaccgctt aaaaaatttt cttgaacgtc cggcagttaa gcggatttca atcacctgcc
     3961 cggccttaga cggagacaac ggagtgaata caaaaaacat gctgcgatat atttccgctt
     4021 taacggttat cgccgctgca gtttactttt tttacctgca attcaaaaaa aacgccgacg
     4081 ccatcggcgc ctggaagttc agcattaacc ccctggagat gagcctggcg gtaattttcg
     4141 gcagttgcgc cctgcttgtc ggacctctcg tctggcgact ttttgtcaac agctatttga
     4201 agaaaaaatt aaactatact gaaagctttg tgctttactg cacatccgcc atgttcaaat
     4261 acatcccggg aaaaatatgg acctatgcgg ctcagatagc cctgctttca tccaaaggaa
     4321 tttctaaggt cgcattgctt tatatcaatc tcgtcagctt tatctgtctg cttttcgtcg
     4381 ccgtgccgtt cttcttgtac tactatttca tttacgttca ggaaatgtcc ctcggggcaa
     4441 ctctgctgat cttaacgctg tttatgctga tggatgttgt tttcattatc gcgaaccata
     4501 ccatcgtcaa ttatctgatt cggcccatca accgattgtt caagttgaac atcaaaccga
     4561 ttcgaaccga caaaatcatt tttgtctatg tacagatgct ttactcgctg gcgtgtgttt
     4621 tgctcgggat cgccctgtat tttcttgccc ggggagtaaa catgccatta ccgttttcgc
     4681 agatcattgc catgatggcg acaatctgcg tttcgctgat tcttggattg ctggcttttt
     4741 ttaccgtggg cggactcggc gtgcgggaag gagccatgtt tttcatgctg aagcagtttt
     4801 ccaatattga agcggccctg atcctcccgg tgatctcccg atttctcata acccttgttg
     4861 aacttttaat ggctgttacc gcaataataa taggtttaaa atacggctat tttcatgata
     4921 tagaaaaagt gaagccagca gccgactccg cattaccgcg cgagtcggct ggatgatcac
     4981 cggacagttt tttaacaacg gaggtaataa ccatgatgac ctttacggat ttattaaaaa
     5041 acagaaggtc cattcgcgat tttcaggaca agaacgttcc catgaacctt atcgaggaaa
     5101 tccttcagga atcgacgttg gcgccttcgg ccagcaacaa tcagccctgc cggttttcca
     5161 tcgtccattg cagaaaaacc atcaaggcgc tttccgatga aagcaaagca aatctcctgg
     5221 aggattacgc cggcaataaa aaatccctcc tgccgcagta catcgatatg ctgaaagatg
     5281 aaaatttcaa tgtcttctac aacgccccct gcgtgatttt catcatcggt tcaaagagcg
     5341 tcaagtcgct tgacgtggat tgcgcgctgg cggcaagcta
//
LOCUS       DAOOLB010000121         5363 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_18378_length_5363_cov_47.957423, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000121 DAOOLB010000000
VERSION     DAOOLB010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18378_length_5363_cov_47.957423"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLF77_10280"
     CDS             complement(<1..461)
                     /locus_tag="PLF77_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC09284.1"
                     /translation="MDFLQVSGKTFLVTGVANKKSVAFFVAKGLVENGAQVIFSVQNE
                     ENKDRAQKLFPDSTVLICDVEKTGSINRLGEYFKNHNIKLAGFVHSIAFANYSEGLKP
                     FHETKPEDFLQAANISCFSLVAVANALKNSFENDASIVTISISNTRATAYG"
     gene            complement(473..889)
                     /locus_tag="PLF77_10285"
     CDS             complement(473..889)
                     /locus_tag="PLF77_10285"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HPC09285.1"
                     /translation="MDEVLAALPQRPPFLFVDKIICRDGRSITTQRTLRADEYFFQGH
                     FPGNPIMPGVLLCEACFQTGAILMVASHQPGLGVVTRIRDIKFKHFARPGDTLTMKVT
                     LDDQVDNAYYMSGTIHIGDKLILKIIFQAALLPPEQ"
     gene            complement(889..1629)
                     /gene="fabG"
                     /locus_tag="PLF77_10290"
     CDS             complement(889..1629)
                     /gene="fabG"
                     /locus_tag="PLF77_10290"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HPC09286.1"
                     /translation="MFDFSNQRVIVTGGTRGIGAGIAEAFLQAGATVIAVYSANDAAA
                     NEFKNKNEQFSDRLDIQKQDISSSQDVKNLFQYLDEKYPSLEVLVNNSGIRQDAIAAL
                     MTDEQWNKVLEVNLTGTFYMSREIIPRFMSGRFGRIINISSLGGDIGFQGQANYAASK
                     AGQLAIAKSLSKEVARKGITVNCVSPGFIDTELIGDLPEEQRKEYLKMIPQKRFGKVE
                     EVAHAVLFLATREASYINGTSITVAGGL"
     gene            complement(1638..3056)
                     /locus_tag="PLF77_10295"
     CDS             complement(1638..3056)
                     /locus_tag="PLF77_10295"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPC09287.1"
                     /translation="MLLNKNDKITQHYDVIIIGGGLGGLTAANKLAKNGRKVLILESH
                     DKLGGFATWFYRKKHIFDVSLHGFPVGMIKTCRKYWNKAIAEKIVQLKRMRFINPMYR
                     LETDYTVEDFTEKLIACFNVSKEQVDNFFSLVMNMNYYDNKAMTNGELLESFFPGRKD
                     VHRFLIEPIVYANGSTLEDPAITYGIVFSNFMSKGVYTFQGGTDLVIGMMQEELMKNN
                     VDIRLKSNVGKIVVENNVCRGVMLGGEFVSSTSVLSNGNLIGTIFNLTGEDNFKPEFI
                     DKVKKVRLNTSSCQVYMGIRQGEAIEDIGDLIFYSEAKEFETDELLSMNTTSRTFSFY
                     YPRLRPQLENAGYTIVASTNARYEDWAELSKEDYKQKKRELIEATLVSLEKIIPGIRQ
                     KIDYVEAATPLTIKRYTHHEGGSSFGTKHEGLDISMKLHHEISGLFHTGSVGIIMSGW
                     LGAANYGVIQSYEVEGYLDKLN"
     gene            complement(3043..4038)
                     /locus_tag="PLF77_10300"
     CDS             complement(3043..4038)
                     /locus_tag="PLF77_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPC09288.1"
                     /translation="MNIIVTGAGGFLGRNLANGLLSKGHKVRNFSRRNHLPLQAMGVE
                     TICGDLQNASEVMAAFQGIDAVFHVASRVGIWGDYDEYYRTNVVGTGNVIKACRANGI
                     TKLIYTSTPSVVFDGNDILRADETKPYALKFYNDYSRTKTLAEQKVLQANDETLSTVA
                     LRPHQIIGPDDPHLVPRLVNAAKRGRLKIVGTGKNLVDVTYIQNAVEAHLLAFEKLSP
                     QSSVAGQAYFIGQERPVVLWDFINKILTRYNLSPITKRVPEKVALNMGALFEAWYRFF
                     KINGEPPMTRFVALQFSRSHYFSHEKAKNDFGYVPKISIEQALDLTTERGMNAFK"
     gene            complement(4035..>5363)
                     /locus_tag="PLF77_10305"
     CDS             complement(4035..>5363)
                     /locus_tag="PLF77_10305"
                     /inference="COORDINATES: protein motif:HMM:NF006754.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031956 - medium-chain fatty acid-CoA
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid CoA ligase family protein"
                     /protein_id="HPC09289.1"
                     /translation="AAIEEVAPEGLIAEPEVHLLRIFFNNSFKSVRFKVTTKGPKIGN
                     CFPLSMLKRARAGFQSAQLEPDETAAILFTSGGTGCPKGVVYTHKIYYEQTRLLQELF
                     SLDENEIDFPCFPLFSLFTLCMGMTSCIPDMNPSQPAKADPKQLVQNIKDNKVTFMAG
                     SPAIWEALGSYCIKNNITLPSVKYLVMFGAPVRNELHKRFSRILPNGTTYTPYGATES
                     LPVANISGQMVLEQTAALTDEGKGTCVGEPAPGVKVAIAAINDNIIPHIRQARILGLH
                     QIGEIIVKGDMVTAGYYQSPQETALAKIADGASFWHRMGDLGYIDDAGKLWFCGRKSH
                     RVETPSGLLCSVMCEAIFNTHNGVRRSALVGIGEKGRQSAAIVIERNSAGRKLDRKRL
                     EKELLGLGGKNPLTAAITSVHFCDSFPVDVRHNIKIDRLKLAEDVARGRI"
BASE COUNT         1316 a         1376 c         1220 g         1451 t
ORIGIN      
        1 tacccatagg cggttgcccg cgtgttgctg atggatatgg tgacaatgga agcatcgttt
       61 tcaaaagaat tcttcagggc attggcgacg gccaccagcg agaagcagga gatattggcc
      121 gcctgcagaa aatcttccgg tttggtttca tgaaatggtt tgaggccttc gctgtaattg
      181 gcaaaggcga tggaatgaac gaaaccggcg agtttgatat tatgattttt aaaatattct
      241 cccagacgat tgatgcttcc cgttttttca acatcgcaga tcaacacggt gctgtcgggg
      301 aaaagttttt gcgctctgtc tttattttct tcattttgaa cggaaaaaat cacctgcgcc
      361 ccgttttcga ccagaccttt ggccacaaaa aacgcaacgc tctttttatt ggccacaccg
      421 gtcaccagaa atgtttttcc tgatacttgc agaaagtcca tatcaacctc tcttattgct
      481 ccggcggaag aagcgccgcc tgaaagatga tttttaaaat gagtttatct ccgatatgaa
      541 tcgtaccgct catataataa gcgttatcca cctgatcatc aagggtaacc ttcatggtca
      601 acgtatcgcc cggacgggcg aaatgtttaa atttgatatc tctaatccgg gtgacaacac
      661 ccaggcccgg ctgatgactt gctaccatta aaatggctcc ggtctggaaa caggcttcgc
      721 acaacaagac gcccggcatg atgggattgc cgggaaaatg gccctgaaaa aaatattcat
      781 cggctcttaa cgttcgttgc gtcgtgatgg aacgaccgtc tctgcagata attttatcga
      841 caaaaagaaa cggcggacgc tgcggcagag cagccagaac ttcgtccatt atagtcctcc
      901 ggcaacggtg atcgatgtcc cattaatgta ggaggcctcg cgggtcgcca ggaacaacac
      961 cgcgtgggcc acttcttcca ccttgccgaa acgtttctgc ggaatcatct tgaggtattc
     1021 cttgcgctgc tcctctggga gatccccgat gagttccgta tcgataaatc ccggagaaac
     1081 gcagttgacg gtaatccctt tcctggcgac ctctttggac aaagatttgg caatggccag
     1141 ttggccggct ttggatgccg cgtaattggc ctgtccttga aatccgatat ccccgcccag
     1201 cgaactgatg ttgatgattc gtccgaaacg accggacata aagcgcggaa taatttcccg
     1261 cgacatataa aacgttcctg tcagattaac ttccaaaact ttattccatt gctcatcggt
     1321 cattaacgcc gctatcgcgt cctgccggat acccgagtta ttcaccagca cttccaatga
     1381 gggatatttt tcatccagat actgaaacaa atttttaaca tcctgcgacg aggagatatc
     1441 ctgtttttga atatcgagtc tatccgaaaa ttgttcattt ttatttttaa attcattcgc
     1501 ggccgcatcg ttggctgaat aaacggcaat caccgtcgcg ccagcctgca gaaaagcctc
     1561 ggcgataccc gctccgatcc cacgggttcc gccggtaaca ataactcttt gattgctgaa
     1621 atcaaacata aaacacctca attaagttta tccagatagc cttccacttc ataagattga
     1681 atgacgccgt aattggcggc gcccagccag ccggacatga taatgccgac cgatccggta
     1741 tgaaacagac cgctgatttc atgatgcagt ttcatggaaa tatccaagcc ctcgtgtttg
     1801 gttccgaacg aggagcctcc ttcatgatgc gtatatcgct tgatcgtcaa cggcgttgcc
     1861 gcctcgacgt agtcaatttt ctgtcggatg ccgggaataa ttttttccag actcaccagc
     1921 gttgcctcaa tcaattcccg cttcttttgc ttataatctt ctttgcttaa ttctgcccag
     1981 tcttcatagc gcgcattggt cgatgccacg atggtatatc cggcattttc cagttggggc
     2041 cgcagtcgcg gatagtaaaa ggagaatgtc cgactggtgg tgttcatgga cagcaactca
     2101 tcggtttcaa attcctttgc ttccgagtag aagatcagat cgccgatatc ctcaatcgct
     2161 tctccctgtc tgattcccat atacacctga caggaactgg tgttgagccg gactttttta
     2221 accttgtcga taaattccgg tttaaaatta tcttctcccg tcagattaaa aatggtcccg
     2281 atgagattgc cgttggaaag aaccgacgtg gaagaaacaa actcgccgcc gagcatgact
     2341 ccccgacaaa cattgttttc aaccacaatt tttcccacgt ttgatttcag acgaatatcg
     2401 acattatttt tcataagctc ctcctgcatc attccgatga cgaggtcggt tccgccctga
     2461 aacgtataaa cgcctttgct catgaaattg gagaaaacaa ttccgtaggt aatggccgga
     2521 tcctccagcg tcgaaccatt ggcgtacaca atcggctcga tcaaaaaacg atgcacgtct
     2581 tttctgcctg ggaagaaact ctccagcagt tcgccgttgg tcattgcctt gttatcgtag
     2641 taattcatgt tcatgaccag gctgaaaaaa ttatccacct gctctttaga aacgttaaaa
     2701 caggcaatga gcttttcggt aaaatcttca accgtataat ccgtctccaa tcggtacatg
     2761 ggattgatga aacgcattct tttaagttga acgattttct ccgctatcgc cttgttccaa
     2821 tacttgcggc aggttttgat catccccacc ggaaatccat gcaacgacac atcaaaaata
     2881 tgtttcttgc gataaaacca ggtagcaaag ccaccaagtt tatcgtgcga ctccaggatt
     2941 aatacttttc ttccgttttt ggccagtttg ttggcggcgg tcaatccccc cagtccgccc
     3001 ccgatgatga tgacatcgta gtgttgtgta attttatcgt ttttatttaa aagcattcat
     3061 tcccctctct gttgtaagat ccagcgcctg ctctatggat attttcggga cataaccgaa
     3121 atcgtttttc gctttttcat gggaaaaata atgggagcgg gaaaactgca gcgccacaaa
     3181 tcgcgtcatc ggcggctctc cgttaatttt aaaaaatctg taccaggctt cgaacaaggc
     3241 ccccatgttc aacgccactt tttcgggaac tctttttgtg atgggagaaa gattatatcg
     3301 cgtcagaatc ttgttgataa aatcccagag cacgaccggc cgttcctggc cgatgaaata
     3361 agcctgtccg gcaacagatg attgaggaga caacttttcg aacgccagga ggtgcgcctc
     3421 aacggcattt tgaatatagg tgacatccac aagattcttg ccggttccga ctattttcag
     3481 tctgccccgt tttgccgcat tgaccagtcg agggacgaga tgcggatcgt ccggaccgat
     3541 gatctggtgg ggcctcaggg cgacagtaga taatgtttca tcattagcct gcaaaacctt
     3601 ctgttcggcc agcgttttgg tccgggaata gtcattataa aatttcaggg catagggttt
     3661 ggtctcgtcg gctctcaaaa tatcattgcc gtcgaaaaca acgctgggcg tgcttgtata
     3721 aatcagtttt gttatgccgt tggcgcggca cgccttgatg acatttccag tgccgacaac
     3781 attcgtccgg taatattcat catagtcgcc ccagataccg acgcgcgatg caacatgaaa
     3841 gaccgcatct attccctgaa atgccgccat aacctcggaa gcgttttgca aatcgccgca
     3901 aatagtttca acgcccatgg cctgcaatgg cagatgattc ctgcggctga agttccggac
     3961 cttgtgtcct ttggacagca agccgtttgc cagatttctt cccagaaaac ctcccgctcc
     4021 ggtaacgata atgttcatat tctcccccgg gcgacgtcct cggccagttt caaacggtcg
     4081 atcttgatat tgtgccggac atcgacagga aagctgtcgc agaaatggac tgacgttatt
     4141 gccgctgtca aaggattctt tccgcccaga cccaataact ctttttccaa tcgtttcctg
     4201 tcaagctttc gccctgccga atttctttca atgacgatcg cagccgattg cctgcctttt
     4261 tctccgattc cgaccagcgc cgaccgtctg acgccgttat gcgtgttgaa aatcgcttcg
     4321 cacatgactg agcataataa tcccgacggt gtttcgacgc ggtgcgattt tctcccgcag
     4381 aaccagagct tgcctgcatc atcaatataa ccgagatcgc ccattctgtg ccaaaacgat
     4441 gcgccgtcgg caatctttgc cagggcagtc tcctgcggag attgataata cccggcggta
     4501 accatgtcgc ctttcacaat gatttctccg atctgatgca ggcccaatat gcgcgcctgc
     4561 cggatatgag gaattatgtt gtcgtttatc gccgcaatgg caactttcac gccgggggca
     4621 ggctctccca cacaggttcc cttgccttca tcggtgagcg cggccgtttg ctccaaaacc
     4681 atttgtccgg aaatattggc tacgggaaga ctctccgttg cgccataagg cgtataggtg
     4741 gttccgttgg gcagaatcct gctgaatctt ttatgcagtt cgtttctgac cggtgcaccg
     4801 aacatcacca gatatttcac cgaaggaagg gtaatgttgt ttttaatgca gtaacttccc
     4861 agcgcttccc agatagccgg cgatccggcc ataaaggtaa ctttattgtc cttgatattt
     4921 tgcaccaatt gcttcgggtc cgcctttgcc ggttgcgagg gattcatatc cggaatgcag
     4981 gacgtcatgc ccatacagag cgtaaagaga gaaaacagcg ggaagcaggg gaaatctatt
     5041 tcattttcat cgagagaaaa aagctcctgt aaaagacgag tctgctcgta ataaattttg
     5101 tgggtataga caacgccttt cgggcagccg gttccgcccg aggtaaacag aatggctgcc
     5161 gtttcgtccg gttccagctg cgcgctttga aaaccggctc tggcccgttt caacatggac
     5221 agcgggaagc aattgcctat cttcggtcct ttggtggtaa ccttgaatcg gaccgattta
     5281 aacgagttgt tgaaaaaaat cctcaggaga tgcacttccg gttctgcaat caatccttcc
     5341 ggagccactt cttcaatggc cgc
//
LOCUS       DAOOLB010000122         5305 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_18654_length_5305_cov_36.581524, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000122 DAOOLB010000000
VERSION     DAOOLB010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18654_length_5305_cov_36.581524"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PLF77_10310"
     CDS             complement(<1..320)
                     /locus_tag="PLF77_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPC09290.1"
                     /translation="MPSILVIDDDASIAETLDLYLTEEGYNVRTALTGTEGLNQYVQE
                     PSDVVILDIRLPDIDGFTVLEDLKEENENVKVIMITAFHDMETTIQAMKSGAFDYIHK
                     PVNV"
     gene            complement(333..2945)
                     /locus_tag="PLF77_10315"
     CDS             complement(333..2945)
                     /locus_tag="PLF77_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC09291.1"
                     /translation="MTLFSKFKILLYKAAQLRILFASVTVVVLFLLLALTVNNSREKD
                     IVNIFSRQQLVNVQNTSKRMMDVFSQIGKNIDLFSRLHPDLTIDEKELDSYYKMLSSG
                     WEDTFDAIVMFDAGGNIKNVYPKNVVPSINVSEHFNVLSKQQKRYLGLALPDASGTLG
                     SKQKHDRYLVLGYPLRKPNGEFSGALLISFSLSAVLHKYRQRPADDELGELWLIDEQQ
                     KILVHPRWAFVGNDIKKFLKKSDEQASMFSSENGDNFEAVVLQGDKIKQRSIISYYPV
                     KAGDKKWTMLVVAPYSKVISPLRKTFFYTLFSSLLLIIIVIATSMSFAYKEGKRLRHR
                     EERKRLKERRDWQEKLLREKKTIEGIIEGSPIPSFVINNKHKVILWNRACSELTGHSA
                     DEMLGTGNHFKPFYSVTRPMIADLIVDSDMDGLSKHYGTKNVKKSEKVVGAYEATDYY
                     ANLGGQSRYLYFLAAPIYDEEGKIIAAVETLQDVSREREMTKSLSEYAETLQNELIEN
                     IELQRQIQDLNNYLQTIITSLPDRIYEIDEKGIINFMSRGLRKKTGTSGEFKNKYFLD
                     FVASGYEDLVLSKWEEAKKGIYKPYEIEAIGKDGRKHNLLITTSPVIGTDHYIIVQRD
                     ITEFKDLERKLYDSQKLAALGQLSAGIAHEIRNPLSSIKMSLQILTKRMKPDGNDLKR
                     FRIAEKEVEHLETLVNDILAFAKPVDPRKTSVDLSKVLEQAIALSEKGITDKKIEIVT
                     QFGKVFPVTVDAAMMTDAFINVIRNAVEALPENGKIVVSLKYADETRHSAAVEITDNG
                     SGIDETDMPHIFNPFFTRKNYGTGLGLSLVKKIIDIHRGTIDISSRKNEGTKVLIVLP
                     LRLDQALSPAANNE"
     gene            3643..4020
                     /gene="atpE"
                     /locus_tag="PLF77_10320"
     CDS             3643..4020
                     /gene="atpE"
                     /locus_tag="PLF77_10320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPC09292.1"
                     /translation="MSHKLGKVILTAFTCMVVLLVASPFVFAAEAIPAGGESYIKVYF
                     AVGAMIGAGLAMGLGAIGAGAGIGNAANGACQAVGRNPGVQGKIMMVMLVGMAMAESI
                     AIYALVISLILLYANPYMRYFLG"
     gene            4068..>5305
                     /locus_tag="PLF77_10325"
     CDS             4068..>5305
                     /locus_tag="PLF77_10325"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HPC09293.1"
                     /translation="MSETKKEKRFFNIGGVFFLLIVIPLSLMAFLIANGMFKLGVTVK
                     EKTVNVLDQKSQEDIKARAVNTANEIAKFLMDTKKDLQVATILPSTEAVFKQFVSENK
                     KPVWVKRDGKVQQILIPLYSEMTLIDKNGNEQIKIVDGQAVPADKRVNVSNPANTVYK
                     TEDYFAKAKNLNKGEIYVSPATGYYVDKAAFEKGKRFEGYVRFATPIFSKDGFAGVIT
                     LALDYRHLAEFTDQLVPTQAEKVFETDAATGNYAYMVDNRGFVISHPADYHIVGLTQQ
                     GTTVPTLSADNAKELAKKGAEALNMFQLGFMDHNIFNIAKEAIVGKSGILMYKYGGLT
                     KFVAYAPIKFYASNLPEPAGFGWVGLGLDVEKYNQEAAKVAKEIEKEAKAWTATVIFI
                     LIASMIILFFIMLLLVRGITR"
BASE COUNT         1462 a         1178 c         1160 g         1505 t
ORIGIN      
        1 tcgacgttga caggtttatg aatataatcg aaagcgccgc ttttcatcgc ctggatggtt
       61 gtctccatgt cgtgaaaagc ggtaatcata atgaccttta cattttcatt ctcttcctta
      121 aggtcttcca gaacggtgaa tccgtcaata tccggcaggc ggatatccag gataaccaca
      181 tcggaaggtt cctgaacata ttgattcaga ccttccgtcc ccgtcaacgc cgtgcggacg
      241 ttatatcctt cctccgtcag atagagatcc agggtctcgg caatggaagc atcgtcgtca
      301 atgacaagaa tactcggcat aatacagtca ccttattcgt tattggccgc aggagaaaga
      361 gcctgatcca gccttaaggg taaaacgatc agcacctttg ttccttcatt ttttctgctt
      421 gaaatatcaa tcgtcccgcg atgaatatca attattttct ttaccagaga aagccccaaa
      481 ccggtaccat aattttttcg ggtaaagaaa ggattgaaga tgtgcggcat atcggtttca
      541 tcaataccgc tgccgttatc cgtgatctcc acagccgccg aatggcgcgt ttcatcggca
      601 tactttaacg acacaacgat tttgccgttt tccggcaacg cttccacggc attgcgaatc
      661 acgttgatga aagcatccgt catcatcgcc gcatcgacag tcaccgggaa aactttcccg
      721 aactgcgtga cgatttcaat ttttttatcg gtaataccct tttcggataa cgcaatggct
      781 tgttccagaa ccttggacag atccacagac gtttttcggg ggtccaccgg tttggcaaag
      841 gccaagatat cattgaccag cgtctccaga tgctccactt ccttttccgc aatcctgaat
      901 cgcttcaaat catttccgtc cggtttcatt cgtttcgtca ggatctgcaa actcatttta
      961 atggaggaaa gagggttgcg tatttcgtgc gcgatgccgg cggaaagttg tcccagggct
     1021 gcgagcttct ggctgtcgta aagttttctt tccagatcct tgaattcggt tatgtcgcgc
     1081 tgaacaatga tgtaatgatc ggtgccgatg accggcgacg tcgtaatcag aagattatgt
     1141 ttacggccgt ctttcccgat cgcttcgatt tcatacggtt tataaatgcc ttttttggcc
     1201 tcttcccatt tggacaaaac cagatcttca taaccggaag caacaaagtc caaaaaatat
     1261 ttgtttttga attcacccga tgtgcctgtc ttttttctca ggccccggct cataaaattg
     1321 atgatgcctt tttcatcgat ttcatagatt ctgtcaggca gactggtaat gatggtttgc
     1381 agataattat tcagatcctg aatctgtcgc tgcagttcaa tattctcaat caattcgttc
     1441 tgcagcgttt cggcatattc gctgaggctc ttcgtcatct ctctttcacg cgacacatcc
     1501 tgcaatgttt caaccgctgc aatgattttg ccttcttcat cgtagatggg ggccgccaga
     1561 aaatacaggt aacggctttg cccgccgaga ttggcataat aatccgtcgc ctcgtaggcg
     1621 cctacaacct tttcggattt tttgacgttt tttgttccgt aatgtttgct gagcccgtcc
     1681 atatcgctgt cgacgatcaa atcggcaatc atgggacgtg tcacggaata aaagggcttg
     1741 aagtgatttc cggttcccag catttcatcg gcggaatggc ctgtcagttc ggaacaggca
     1801 cgattccaca agatgacttt gtgcttatta ttgatgacaa agctgggaat gggcgatcct
     1861 tcgatgatgc cctcaatcgt ctttttttcc cgaagcagtt tttcctgcca gtctctgcgc
     1921 tccttgagcc gttttctttc ttcccggtgg cgcagtcttt ttccttcctt ataggcaaaa
     1981 gacatgctgg tcgctatcac gatgatgatg agcagcaggg agctgaaaag ggtgtagaaa
     2041 aaagtttttc tcaacgggga gatgactttg ctgtagggag caacaaccag catcgtccat
     2101 ttcttatcgc cggccttaac gggataatag gagatgatgc tccgttgttt tattttatca
     2161 ccctggagga caaccgcttc aaagttatcg ccgttctcgg atgaaaacat cgatgcctgt
     2221 tcgtccgatt ttttaagaaa cttttttata tcgtttccga caaaagccca acgaggatga
     2281 accagtattt tttgctgttc atcgatcagc cacaattctc ccagttcatc gtcggcgggt
     2341 cgttgccggt atttatgtaa aactgcggaa agagaaaatg aaatcagcaa cgccccgctg
     2401 aattcgccat ttggcttccg gaggggatac cccaaaacca gatatctgtc gtgtttctgc
     2461 tttgaaccga gcgtcccgga agcatccggc agagccaggc ccagataacg cttttgctgt
     2521 ttactgagca cattaaaatg ctcggataca ttgatcgacg gcacaacgtt tttcggataa
     2581 acgtttttga tgtttccgcc ggcatcaaac atgacaatcg catcaaaagt gtcttcccac
     2641 ccggaagaca acattttata ataactatcc agttcctttt catcaattgt caaatcggga
     2701 tggaggcggg agaataaatc aatattttta ccgatctggg aaaaaacatc catcattctc
     2761 ttggaggtgt tttgaacatt gacaagctgc tgccggctga aaatattgac aatgtctttt
     2821 tcgcgggaat tgtttaccgt cagagccaga agcagaaaaa gaaccacaac ggtaacactg
     2881 gcaaacaata tgcgtaattg tgcagcttta taaagaagga ttttaaattt gctgaataaa
     2941 gtcacctaat ctttttaatc ctctttgttt tctttatggg aaagctttat tccgatcagg
     3001 acataaacca gaattgccgc cgttaatgaa ataagaagtt tggccaagcc gctttcaatt
     3061 cttaaaacat acgaacagat aagccatacg atagggtaag ttattgccgt taaaagctcc
     3121 tgcataatca tcctttataa cgcgtctctt aattttattt tgacgatagt aacggccttg
     3181 gacgaaaatt tcaataaaaa tataagaaag aacgtttcct tattcatcag tgcgctgtga
     3241 ccactagcag caagagaaaa aaatcaaccc ccgtatacgg aagtattctg ccgaaagcca
     3301 cttgaatgtt tttttatagc cgtatttaac aaaacattaa acgtcgcggc aaaggaatgc
     3361 ttttaaaatg atcaaaaaac attttggata tatttttcgg atatcttttt tatgtcggtt
     3421 aagcgcgtca aatgaaataa tctcaataca ttagatatgc ctcttccttg tgacctcctt
     3481 tttcatcttt gaaaaatatt ttcatatcaa agatatggca caactcatgc agtaatcttg
     3541 ccgcagcaag aggtaaaatt atttcgcttt ccatcgaatg ttaatttatg ttatggaaaa
     3601 aagaagtaat aataaacatt ttattatgaa aggagacaaa caatgtcaca caaattgggt
     3661 aaagtaattt taacagcatt tacatgcatg gtggttctgc tggtagcaag tccattcgta
     3721 tttgcggcag aagctattcc ggcaggtgga gaatcttaca ttaaagtgta ttttgctgta
     3781 ggcgcaatga ttggagccgg tctggcaatg ggactgggcg cgatcggcgc aggcgcaggt
     3841 atcggtaacg ctgcaaacgg cgcatgtcag gcagtaggaa gaaaccccgg tgttcagggc
     3901 aagatcatga tggttatgct cgtcggtatg gccatggctg aatccatcgc catttacgct
     3961 ttggttattt ctctgattct tctctatgct aacccttaca tgcgttattt tttgggttaa
     4021 tataaaacaa ttaactacaa aataacaaag gttaggggga aatagtaatg tcagaaacta
     4081 aaaaagaaaa acggtttttc aatataggag gcgtgttctt cctgcttatc gtaatcccct
     4141 tatcactaat ggcgttttta atcgctaacg gcatgtttaa actgggggtt accgtaaaag
     4201 agaagacggt aaacgttctc gatcagaaat cccaggagga tataaaagca agagccgtca
     4261 acacggcaaa tgaaattgcc aaattcctga tggataccaa aaaagattta caagttgcaa
     4321 cgattcttcc ctcgacagaa gctgtattca aacagtttgt ttctgaaaac aaaaaaccgg
     4381 tgtgggtcaa acgcgacggc aaagttcagc agatattgat cccgctctac agcgaaatga
     4441 ctctgattga caaaaacggc aacgaacaga tcaaaatcgt tgacggtcag gcggtgcccg
     4501 cagacaaacg tgttaatgta agcaatcctg ccaatacggt atataaaacg gaagattatt
     4561 ttgccaaggc aaaaaatctg aataaaggtg aaatttatgt ttcccccgca accggctatt
     4621 atgtcgacaa ggcagcgttt gaaaagggaa aacggtttga aggatacgtc cgttttgcaa
     4681 caccgatatt cagtaaagac gggtttgccg gcgttattac actggcattg gattatcgtc
     4741 atctggccga atttacggat cagcttgttc ccacacaggc cgaaaaggta ttcgaaacag
     4801 acgcggcaac gggtaattat gcgtatatgg tcgataatcg cggattcgtc atttctcatc
     4861 cggcggatta tcacattgtc ggactcaccc aacagggcac aacggtcccg acgctttcgg
     4921 ctgataatgc caaagaactg gccaagaagg gtgcggaagc gttgaacatg ttccagcttg
     4981 ggtttatgga tcacaacatc ttcaatatcg ccaaggaagc tattgtaggg aaatcgggaa
     5041 ttctgatgta caaatacggc ggactgacga agtttgtcgc ttatgcacct atcaaattct
     5101 acgcatccaa tctgcccgaa ccggccggat tcggatgggt cggactgggg ctggatgttg
     5161 aaaagtataa ccaggaagct gccaaggtcg ccaaggaaat tgaaaaagaa gccaaagcct
     5221 ggacggcaac ggtcatcttt atcctgatcg cttccatgat tattctgttc ttcatcatgc
     5281 ttctcctggt tcgcggaatt acccg
//
LOCUS       DAOOLB010000123         5122 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_19570_length_5122_cov_40.092560, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000123 DAOOLB010000000
VERSION     DAOOLB010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19570_length_5122_cov_40.092560"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLF77_10330"
     CDS             complement(<1..184)
                     /locus_tag="PLF77_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09294.1"
                     /translation="MKNELEIIGNGQEINTKQKRAIAYLVAAKDIESGCRAAGISTTT
                     YYDWIKNPVFADAIDTQ"
     gene            complement(174..341)
                     /locus_tag="PLF77_10335"
     CDS             complement(174..341)
                     /locus_tag="PLF77_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09295.1"
                     /translation="MKQNKLLRRLEALEEIVKPKVTEIPPEAQAIIDRMLAARAERLK
                     ITTPEDEKHEK"
     gene            complement(407..799)
                     /locus_tag="PLF77_10340"
     CDS             complement(407..799)
                     /locus_tag="PLF77_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09296.1"
                     /translation="MKNQKKAFVDQGKRIVLKYNEDVTGAACYFCGLRVEDDGFDFFV
                     EGTNEFRVCEHCAKEKAPDLYEIWRDAHRWNEEIDIKSFREGVKVGKEEAGQMIIDTI
                     DEPVISRVRRICQVDLDAKPFREVDIPF"
     gene            complement(1012..1389)
                     /locus_tag="PLF77_10345"
     CDS             complement(1012..1389)
                     /locus_tag="PLF77_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09297.1"
                     /translation="MSLFKLLKLKTKAEKANGSQRSSTDVDLHAPATYEMAVEMAYIL
                     TTTRIEVEAYLSGGRFVNNDHLRRLLQNVDDIGDQALAGTKRFEDYVDAVEKWKQAIF
                     QSHPRADKMTSDIENCNLMEKAI"
     gene            complement(1620..3440)
                     /locus_tag="PLF77_10350"
     CDS             complement(1620..3440)
                     /locus_tag="PLF77_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09298.1"
                     /translation="MKTGMDDFLNTHSVEDFASLPETEVTDEAIARNGNRQPYYIADD
                     GSTYRWKDTKDGRYRVKLANFAAIISEETREDDGVEVTHSYKIAGYADKQFFPAIEVP
                     AGQFGAMNWVTKWGTEAIIEPGSNNKDCMRHAIQILSRGRTRKTVCFTHTGWRYTDGQ
                     WIFLTSTGAIGKEGVNVRLSRENARYSLPLAPENEKEAIEASLSFLQIGKKEITYPLF
                     AVTYLSPLTSLLNPPPNFISYVYGQTGTFKTTTTLLQLSHFGDFQSITSLSNFDDTGN
                     SIEKKGFTLKDVLHVLDDYHPSFRRQDAQAKESLAQRAIRAYANRTARGRLNADTSDK
                     GRHEPRGFLQITGEELVSLPSTQARTLVVEIEPGDIDKDKLTTIQDKMDLLPHAMTSF
                     LLWVRDNIPEIQSGFPSRFRELREIAADAAGNGIHRKLPEQTAFLLYALELVGQWLMD
                     KGIHSEDEAEAFVKEAFDIFNKLTDRQAQRIAEDDPVKKFFDILSALVLSNNAQFDHM
                     QRQGVTRGGGDVIGYFDDEYTYLIPTPTWHAISRYSMQEGNHFPFKPRSLYKLLRAKD
                     ILIPYKNESTTPVKINGSLHRVIKIYGTYLNLNVTPVTEG"
     gene            complement(3421..4056)
                     /locus_tag="PLF77_10355"
     CDS             complement(3421..4056)
                     /locus_tag="PLF77_10355"
                     /inference="COORDINATES: protein motif:HMM:NF024367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3854 domain-containing protein"
                     /protein_id="HPC09299.1"
                     /translation="MRLIMTIIAHPKHLADLRKSGLTDETISRAKIESIRPDRIGKEL
                     GYSLQNLESMYGIPYDDQFSRYRCFYFEGTTGSKYLQVKNSGNRLYIPPNIVRDKLQD
                     ADATLYITEGEKKALRACQEGLLCVGLSGLWNWKNKAKDDLIDDFNLINFKNRIVKLV
                     PDDDWLTPNKHGYKKNLQAAVYRLAGKLKLRGARVFIVNLEKENKNENRNG"
     gene            complement(4053..4286)
                     /locus_tag="PLF77_10360"
     CDS             complement(4053..4286)
                     /locus_tag="PLF77_10360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09300.1"
                     /translation="MQNELAYHDVYSVAYADLKGIPIRMIREDRRVIFLLPDEPNTYQ
                     VLGEFNSNPSLPLLNYITHLKKIRAQMIALQRA"
     gene            4474..>5122
                     /locus_tag="PLF77_10365"
     CDS             4474..>5122
                     /locus_tag="PLF77_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HPC09301.1"
                     /translation="MKMNRENVDVLCEKVINAIKKDRAFNKESLLKFLEKEKYKEPYE
                     EFIVNLIPIAAEQGKQEHIQQFLIHHCFEDMEKYIARTDKEIFPSALSSFCLLAAGIT
                     DLGYSIIVPFVEKNWLQHLYYDAQMGDNILIVGETGTGKQAMARAIHLMSERRKLPFE
                     EINCAAIPENLLESELFGHAKGAFTGATGKKTGKLVQAGKGTIFLDELGKMPLHLQ"
BASE COUNT         1269 a         1184 c         1102 g         1567 t
ORIGIN      
        1 tctgcgtgtc tatggcatcg gcaaagaccg gattcttgat ccaatcgtaa taggttgtcg
       61 tgctgatccc ggcagcccgg caaccggatt caatatcctt tgcggcaacc aagtaagcta
      121 tggcccgctt ctgttttgtg ttgatttcct gcccgtttcc aatgatttct aactcatttt
      181 tcatgctttt cgtcctccgg tgtggtgatt ttcagccttt cagccctagc cgctaacatc
      241 cggtcaataa tcgcctgcgc ttccggtggt atttcagtta ctttgggttt gacgatctct
      301 tccaatgctt ctaatcggcg cagtagttta ttttgtttca taactcaccc ctttgcatgg
      361 ccataatttg ccattttata ttcttccttc attttttccc gccttgttaa aaaggtatgt
      421 ccacttcccg gaaaggctta gcgtccaagt caacctggca aatccgcctt actctggaaa
      481 tgaccggctc atcaatcgtg tcaatgatca tttgcccggc ctcttctttg ccaaccttga
      541 cgccttcccg gaaagactta atgtctattt cttcattcca gcggtgcgcg tcgcgccata
      601 tttcatataa atccggcgct ttttctttcg cgcaatgctc acaaacccga aactcgtttg
      661 tgccttcaac aaaaaaatca aatccgtcat cttcgacacg caacccgcaa aagtaacaag
      721 cagccccggt aacgtcctca ttgtatttca acacaatccg tttcccctga tccacaaacg
      781 cctttttctg atttttcatt tcgtttcccc cttttaaatt attgtcctgt tgtttccttt
      841 gctttggttc gtccgttgaa accgcacccg gcattcccgc aagatttcgt ttcgtaatgg
      901 ttcccgcaag tcaaccttac gccaaagcca tttaatgtat gagtccggca gttcgtctaa
      961 ttcgcagccg tgatatttcc cgaaaggcat tttcattagg ttagcaccac ctcatattgc
     1021 tttctccatt agattacagt tttcaatgtc gctcgtcatt ttgtccgctc tcggatgaga
     1081 ttgaaagatt gcctgtttcc acttctcgac tgcatccacg taatcctcaa agcgctttgt
     1141 cccggccaga gcctggtcgc caatatcgtc aacattctgc aaaagacgcc gcagatggtc
     1201 attgttcaca aaacgccccc cggacaggta ggcttccacc tcaatgcgtg ttgtagtcaa
     1261 aatataggcc atctcaacgg ccatttcgta ggtggctgga gcgtgcagat cgacatcggt
     1321 ggatgagcgt tgtgagccat ttgccttttc tgctttagtt tttaatttca acagtttgaa
     1381 tagtgacata ttcaccctct taaaaaatat ttagaccgtt gtgtaaaaat gccgtaactt
     1441 ccgtaaccat aaccttaata attgatttta ttgatggttt catctttcgt ttcccgtaac
     1501 tattccgtaa ctcggcagcg tgttcccgta actctaaaaa ttcggttacg tgaattgtgc
     1561 gtccggttac gtgttggtta cgtgaagaat aattgtttaa tgtgagtaat atcaattatt
     1621 taaccttcgg ttacgggagt tacgtttaaa ttaaggtacg tcccatagat ttttatgaca
     1681 cggtggagac tgccgttaat tttaaccgga gttgtgcttt cgtttttata tgggataagg
     1741 atgtctttgg cccgcaggag tttataaagg cttcgcggtt taaacgggaa gtggtttccc
     1801 tcctgcattg aatatcggct gatggcgtgc catgtcggag tgggaatcag ataagtatat
     1861 tcgtcgtcaa aataaccgat gacatctccg ccgcctctag ttaccccctg acgttgcata
     1921 tggtcgaatt gcgcattatt tgagagaacc agcgctgaaa gaatgtcgaa aaacttcttg
     1981 accgggtcat cttcggcaat ccgctgtgcc tgccgatctg tcagtttgtt gaaaatatca
     2041 aatgcttctt tgacgaaggc ttctgcttcg tcttcagaat gaatgccctt gtccatcagc
     2101 cattgaccga caagttccag cgcgtataga aggaatgcgg tttgctccgg cagttttcga
     2161 tgtattccgt tccctgcggc atccgcggca atttcccgca actcccgaaa ccgcgatgga
     2221 aaccctgatt gtatctccgg gatattgtca cgaacccaaa gcaggaaaga ggtcatggca
     2281 tgcggcagca agtccatctt gtcctggatg gttgtaagtt tatctttgtc gatgtcgccc
     2341 ggttctatct ccacaaccag cgttcgtgcc tgcgtagatg gcaatgacac aagctcctct
     2401 ccggtaattt gcagaaagcc cctcggttca tgtctgcctt tatcggatgt gtcggcattc
     2461 aatcgccctc ttgccgttcg gttggcgtag gcacgaatag cccgctgcgc cagactttct
     2521 tttgcctgtg cgtcctgccg tctgaaggat ggatgataat cgtcaagaac gtgtaatacg
     2581 tctttcagag taaaaccctt tttttcaatg ctgttgcctg tgtcatcaaa gttgctcaaa
     2641 ctggttatgc tttggaaatc tccaaaatga gatagctgca agagagtggt tgtggtttta
     2701 aaagttcctg tttggccata gacgtaagat atgaagttcg gcggcggatt cagtaaactg
     2761 gtcagaggag acaagtaagt gactgcgaac agcggatatg tgatttcctt tttcccaatt
     2821 tgcagaaaag atagggaagc ctctatagcc tctttctcat tctccggtgc caacggaagg
     2881 ctatagcgcg cattttcacg cgacagcctg acattgactc cctctttgcc gattgcgccg
     2941 gttgacgtca gaaatatcca ttggccgtct gtataacgcc agcctgtatg ggtaaaacaa
     3001 acagtcttcc tcgttcggcc acgggacaga atctggatgg cgtgccgcat acagtcctta
     3061 ttgttagaac ccggttcgat gatggcttca gttccccatt ttgtaaccca gttcatcgcg
     3121 ccaaactgac cggcaggaac ctcaatggcc gggaagaatt gcttgtctgc ataaccggct
     3181 attttgtaag aatgagttac ttccacgccg tcatcttccc gtgtctcttc agatatgata
     3241 gcggcaaaat tggccagctt gacccgatag cgtccgtctt tggtgtcctt ccagcgatag
     3301 gtggaaccgt cgtcggcaat ataataaggt tgcctgttgc catttcgggc aatagcctca
     3361 tctgtgactt ctgtttccgg cagtgacgca aaatcttcga cgctgtgagt gtttaaaaaa
     3421 tcatccattc ctgttttcat tcttattttc cttttcaaga tttacgataa atacccttgc
     3481 gccccgcagc ttcaattttc ccgcgagacg ataaaccgcc gcttgcagat tttttttgta
     3541 accgtgtttg tttggcgtca gccagtcatc gtctggcacg agcttgacga tgcggttctt
     3601 aaaattaatc agattgaaat catcgataag gtcatcttta gctttgttct tccagttcca
     3661 cagtcctgat aggccgacac agagcaaacc ctcctgacag gcacgcagcg cctttttttc
     3721 gccttcggtg atgtaaagcg ttgcatcagc atcctgtagc ttgtcacgaa cgatgttcgg
     3781 cgggatatag agcctgtttc cggaattttt tacttgaaga tacttgctgc cggttgtgcc
     3841 ttcgaaatag aaacatcggt agcgcgagaa ttgatcatca tatggaatcc catacatgga
     3901 ttccaggttc tgtagagaat aaccaagttc tttgccaatt ctatccggcc tgatactctc
     3961 gattttagcc cgcgagatag tttcgtctgt aagccctgat ttcctcaaat ctgctaaatg
     4021 cttcgggtgt gcaatgatag tcatgattaa cctcacgcac gttgcagagc aatcatctgc
     4081 gcccgaattt ttttcaaatg ggttatataa ttcagcagag ggagagacgg gttggaatta
     4141 aattctccca acacttggta ggtgtttggt tcatccggca gcaggaagat aacacgccta
     4201 tcttccctga tcatcctgat aggaatccct tttagatcgg catatgcaac ggaataaaca
     4261 tcatggtagg caagttcatt ttgcattttc aattctcctt ttcgtatttg gctttttata
     4321 ttgcgtgatt catgccaaat agataaaaat tactgatgat attgatataa ttatgcaatt
     4381 ggttatttta ctgctgagtg tgttcggttg cttattgaca aattaatata aataattata
     4441 ataaaaagta taattcatat taagggtacg gaaatgaaaa tgaatagaga aaatgtagat
     4501 gttttatgtg aaaaagtcat caacgccatt aagaaggatc gtgcattcaa caaagaatct
     4561 ctcctgaagt ttttagaaaa agaaaaatac aaagaacctt atgaagaatt tatcgtaaat
     4621 ttaatcccta ttgccgcaga gcaagggaag caggaacata ttcaacaatt cttaattcat
     4681 cactgttttg aggatatgga aaaatatatt gctagaacgg ataaggaaat attccctagt
     4741 gctttatctt ccttttgtct tttggcggca ggaataactg atttaggtta tagtattatt
     4801 gtccctttcg ttgagaaaaa ctggcttcag catttatatt atgatgctca aatgggtgat
     4861 aacattctaa tcgttggaga aacaggcacc ggcaaacagg caatggccag agcgatccat
     4921 ttgatgagtg aaagacgcaa attgcctttt gaggaaatca attgtgcggc catcccggag
     4981 aatcttcttg agtctgaatt gttcggccat gcaaaaggtg ccttcactgg agccaccggc
     5041 aaaaaaactg gaaaattagt acaggctgga aaaggaacta tttttttgga tgaactaggg
     5101 aagatgcctt tgcacctaca gg
//
LOCUS       DAOOLB010000124         5023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_20087_length_5023_cov_39.327496, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000124 DAOOLB010000000
VERSION     DAOOLB010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20087_length_5023_cov_39.327496"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(59..2002)
                     /locus_tag="PLF77_10370"
     CDS             complement(59..2002)
                     /locus_tag="PLF77_10370"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPC09302.1"
                     /translation="MINKLLSPAKTEVLFMGNEAIARGALEAGVSVVSGYPGTPSSEV
                     IERLSEVARERNLYVEWSTNEKVALEVAAAASFSGLRSMCVMKQNGVNVASDFLLHLA
                     SSGTRGGIVLVECEDPGALSSVNEGESRYFARMLEIPMLEPANFQEAKDLTRWAFELS
                     EKIRNIVMVRSVTRMSHASGNVICGKLPAKFRTARFISDGSVMDPLRGPVVSTPVVIK
                     HGLQQKKLQTAAELFEKSPFNSYVGPKKPELLIITSSACFLYSREALEILGLQKRVGI
                     LKLSCTWPLPEKLLKKYLRLTKKIMIVEEVLPFLEENIKVAAMTMLKDIGIKTFYGKT
                     DKTLPSAGELNPEIVMRALSKIMKVKLQSLPAGYLKEVQKVAPKIAPRAQTFCPGCPH
                     RASFWNINTALKLDGREGIVCGDIGCYAMASLWPEIGFHTVRTLHSMGSGTGLASGFA
                     KLSAFGLDKPVMAVCGDSTFYHAVIPALINAQHNQADITFIVLDNAGTAMTGFQPHPG
                     INESAIGEPLPPVDIEAVCRAIGAKVKVSDPFDLEGTSRILTSFMAERGSMKVLIMKQ
                     ACALSPEKKGKKQYQMKVNEDLCLGDQCGCNRLCTRIFRCPALTWDRKKQKAVIDDVI
                     CAGCGVCYSICPQKAIARVEAEA"
     gene            complement(2087..3193)
                     /locus_tag="PLF77_10375"
     CDS             complement(2087..3193)
                     /locus_tag="PLF77_10375"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPC09303.1"
                     /translation="MSEDIAILIAYVLLALLFSFLCSIAEAVLLSITPSYIEELKEKN
                     PKRAALLKRLKQDNVDRSLAAILTLNTIAHTVGAIGAGAKATVVFGSAWFGIFSAVMT
                     LMILFLSEIVPKTIGAIYWPKLVVPTVFYVQTLIVTLFPIVWISERMTKFISRGKNLH
                     IFSRDEFVAMARVGEQSGQIDDNEARIIKNLFRFGSLNVTDIMTPRTVIAALSEDKTV
                     ADAAEYVVKIPFSRIVIYKKDIDDITGFVLRDDVLLRDNQERGGEALKTLKREILVVP
                     ESAVLTDLLEIFLKERQHIAIIVDEYGGTTGLVTLEDLIETLMGLEIVDEMDTVVDMR
                     VLARKKWEERAGALGIKINVDGKDQSKKENALPE"
     gene            complement(3497..4648)
                     /locus_tag="PLF77_10380"
     CDS             complement(3497..4648)
                     /locus_tag="PLF77_10380"
                     /inference="COORDINATES: protein motif:HMM:NF016328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HPC09304.1"
                     /translation="MMTGSQKFLKKHVLDEGLCTGCGACVGICPYQVIYHDRTVQLHG
                     CDLVKGRCYDYCPRTPCDVGAIRERYCAAGDLTPEIGAVKGYYFSRAADPGLRNIGQH
                     GATVTAMLELALSEGLIDAAVVSSRDQLSLRKGVIVQNREAIRTHAGSQFTVSATVAA
                     FHQLVTKHTGQVGIVATPCQALALAKMKFNPGHEDIARVNQLTLVIGLYCGWTLSEEK
                     YLKLLTDKGIALESITEMDIPAGRNVLEIRTEDRLHSIAMEEAQTCIREACLYCMDST
                     AEFADVSVGSARFSGNWEDVRQWNQLIVRTTKGKELTDLAVEKGVLELREAPADSLKE
                     LKEAAVNKKKNALKNIIQKSGSAKNLLYLDSRDPAVLKYLEAEKKKSKK"
     gene            4948..5023
                     /locus_tag="PLF77_10385"
     tRNA            4948..5023
                     /locus_tag="PLF77_10385"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4981..4983,aa:Asn,seq:gtt)
BASE COUNT         1205 a         1346 c         1118 g         1354 t
ORIGIN      
        1 ccctgcccgc cgacaccggt gataataatg tttgttggat ttttcttaag agactttttc
       61 atgcttcagc ctccactctg gcaatcgcct tttgcgggca gatggagtag cagacgccgc
      121 agccggcgca gatgacgtcg tcaatcacgg ctttttgctt tttccggtcc caggtcagcg
      181 ccggacagcg gaaaattcta gtgcacaaac ggttgcagcc gcattgatcg cccaggcaaa
      241 gatcttcatt gactttcatc tggtactgtt tcttgccctt cttttcaggg ctcaaagcgc
      301 aagcctgttt catgattaac actttcatgg agccgcgctc ggccatgaac gatgtcagta
      361 ttctgcttgt tccttccaga tcaaacggat cgctcacttt aacctttgcg cctattgcgc
      421 ggcagaccgc ttcaatatcc acgggaggca gtggttcgcc tatcgcgctt tcattaatac
      481 cgggatgtgg ctggaacccg gtcatcgccg ttcccgcgtt gtccagcacg ataaaggtga
      541 tatcggcctg gttatgttgg gcgttgatca gcgccggaat aacggcatga taaaacgtgg
      601 aatcgccgca aaccgccata acgggcttgt ccagaccgaa agccgaaagc ttcgcaaagc
      661 cgctggcgag ccccgtgccc gaccccatgg aatgcagcgt gcgcacggta tggaaaccga
      721 tttccggcca gagcgaggcc atcgcatagc agccgatatc cccgcagaca atgccttcgc
      781 ggccgtcgag tttcagcgcc gtgttgatgt tccagaaaga cgcacggtgc ggacagcccg
      841 gacagaacgt ttgcgcccgc ggagcaatct tcggcgccac tttttgaact tccttgagat
      901 aaccggcagg caaggactgg agtttgacct tcatgatttt gctcagagcc cgcataacta
      961 tttcgggatt caattcgccg gctgaaggca gggtcttgtc cgttttcccg taaaacgttt
     1021 tgatgcctat atctttcagc atggtcatcg cggctacttt gatgttttcc tccagaaacg
     1081 gcagtacctc ttccacaatc ataatctttt tggtcaggcg cagatatttt ttcaaaagct
     1141 tttccggcaa cggccaggta cagctcaatt tcaaaattcc gacacgtttt tgcaagccca
     1201 gtatttcaag agcctcccgg ctgtatagga agcaggcgct gctggtgatg atcaaaagct
     1261 ccggtttttt aggaccaacg tagctgttga aaggactctt ttcaaaaagc tccgctgctg
     1321 tctgtaattt tttctgttgc aggccgtgtt taataaccac cggcgtgctg acgaccggcc
     1381 cgcgcagagg atccatgacc gatccgtcgg aaataaagcg ggcggtgcgg aatttagccg
     1441 gcaacttgcc gcagatgaca ttaccgctgg cgtgcgacat acgcgtgacc gagcgcacca
     1501 tgacgatatt tcggattttc tcggataact caaaggccca ccgggtcagg tccttggctt
     1561 cctgaaaatt ggcgggctcc agcataggaa tttcgagcat gcgcgcgaaa tagcgcgatt
     1621 caccctcgtt gacactggag agtgcgccgg ggtcttcaca ttcgaccagc acgatgccgc
     1681 cgcgtgttcc cgacgaggcc agatgcagaa gaaaatcgga ggcaacgttg acgccgtttt
     1741 gcttcatcac gcacatggaa cgcaaaccgg aaaacgatgc ggcggcggcc acttccagcg
     1801 ccaccttttc attggtggac cactccacgt agagatttct ttcacgggcc acttcagaca
     1861 ggcgttcgat cacttccgaa gacggcgtgc cgggataacc ggaaacgaca cttacgccgg
     1921 cctccagagc cccgcgcgcg atggcttcgt tgcccatgaa caacacttct gttttggccg
     1981 gtgataataa tttatttatc attttataat tcacttaaaa gtatttctat catttccgac
     2041 gctgcccgga agtttagttt cttattatac agaatcggct tctttattat tccggtagag
     2101 cattttcttt tttggattga tctttcccat ccacgttaat ttttatgccg agcgcccctg
     2161 cccgttcttc ccattttttt ctggccaaaa cacgcatatc cacaaccgtg tccatttcat
     2221 caacaatttc caaacccatt aatgtttcga tcagatcttc gagcgtaaca agccctgttg
     2281 ttcctccgta ttcatcgact ataatggcga tgtgctgacg ttcttttaga aaaatttcca
     2341 gtaaatccgt aagtactgca gactcgggaa caaccaatat ttctctcttt aaggtcttga
     2401 gcgcctcgcc acctctttcc tgattatccc gcagcagcac atcatctctc aaaacaaacc
     2461 cggttatatc gtctatatcc ttcttatata tcacaattcg tgaaaatggt atttttacaa
     2521 catattccgc cgcgtcggct accgttttat cctcggaaag tgccgcgatc accgtgcgtg
     2581 gagtcatgat atccgttacg ttgagcgacc cgaaccggaa gagattcttg ataatccgcg
     2641 cctcgttatc gtctatctgt ccggattgtt cgcccactct tgccatggcg acaaactcgt
     2701 ctctgctgaa aatgtgcaga ttctttccgc gcgaaataaa ttttgtcatc ctttctgaaa
     2761 tccaaacaat cggaaacagc gttacaatga gggtttgtac gtaaaatacc gtgggcacaa
     2821 cgagtttggg ccagtaaatg gcgccaatgg ttttaggaac gatttctgaa agaaaaagga
     2881 tcattaatgt cataaccgct gaaaagattc caaaccatgc gcttccaaac acgaccgtag
     2941 cttttgctcc cgccccgatg gcgcccaccg tatgcgcgat ggtgttcagt gttaaaatcg
     3001 ccgccagcga gcgatccaca ttatcctgtt tcagtcgctt caacagggcc gcccgcttgg
     3061 gatttttttc tttcagttcc tcgatatatg aaggtgtaat gcttaataaa acggcttccg
     3121 ctattgaaca caggaatgaa aataacaaag ccagaagaac ataggctatt aatatcgcaa
     3181 tgtcctcact catattaccg atactgaacc gccctgaagc cacggagcaa aaagcagagc
     3241 tcgaggcaaa cataaatatg gaaacagaaa ataaaatcaa acgtaaaatc ttatgaatat
     3301 atttcataaa agttgtcatg ccgagggtgt aatatcattt cattattctt gacatttcat
     3361 tgattttctc ttgtttaccc cgccgttccc ccccctcaag ctcagtcgag tcttttggac
     3421 ccgactgatt tttttattga cagccaagtc aaaaagactt ggctgaacag aggcacaaat
     3481 attcacttct caccattcac tttttacttt ttttcttttc agcttccaaa tatttcagca
     3541 ccgccggatc gcgactgtcc aagtataaca gattttttgc cgagccgctc ttttggataa
     3601 tatttttcaa cgcatttttc ttcttattga ccgccgcttc cttcagttct tttaaactgt
     3661 cggcgggcgc ctcccgcaat tccagaacgc ccttctcaac ggccagatcc gtcagttctt
     3721 tcccttttgt cgttctgacg atcaactgat tccactggcg aacgtcttcc caatttcccg
     3781 aaaaccgcgc cgaaccgacc gacacatcgg cgaattccgc cgtgctgtcc atacaataca
     3841 gacaagcttc ccggatacag gtttgcgctt cctccatcgc aatcgaatgc agccgatctt
     3901 ccgtccggat ctccagaacg ttcctgccgg cggggatgtc catttcggtt atggattcca
     3961 aagcgatgcc tttatccgtc agtaacttta aatatttttc ttcggaaagc gtccagccgc
     4021 agtacaaacc gattaccagc gtcagttgat tgactctggc aatgtcttca tgtcccggat
     4081 tgaatttcat cttggccagg gccagcgcct gacacggcgt ggcgacaatt ccgacttgac
     4141 cggtgtgctt cgtcaccagt tggtgaaaag cggctaccgt cgccgatacg gtaaattgac
     4201 tgccggcatg tgttctgatc gcttccctgt tttgtacaat aacgcctttt cgcagcgata
     4261 attggtcccg ggaagaaaca acagccgcat caatcaggcc ttcagacagg gccaactcca
     4321 gcatagccgt tactgttgct ccatgctggc cgatgtttcg taacccagga tcagcagctc
     4381 tggaaaaata atatcccttg accgcgccga tttccggcgt cagatcaccg gctgcgcaat
     4441 atcgttccct gatggctccg acatcacagg gcgtccgggg acaataatca taacaacggc
     4501 cttttaccaa atcgcagccg tgtaattgca ccgttcggtc atgataaatc acctgatacg
     4561 gacaaatgcc cacgcaggcg ccgcaaccgg tgcataaccc ctcgtccaaa acatgttttt
     4621 tcaagaattt ctggctgcct gtcatcattg ccaaccttat ttgccgttct gattcataaa
     4681 agtaggcttt tatatcctat cgtgattttt gaggcaagga aatgatacgg cctgatgaaa
     4741 catttcggcg gtcaattttc cgaacattgc aaaagaattc ggcatttttc atataaagat
     4801 ccggtttttt atcattttga ataaatattt aattgatgtt gacatctgtc tcttcttgta
     4861 ttatgaagcg ccagaaaaaa atgcttcggc aatccgtcat gtcggatcaa tacccggggc
     4921 aaaaattcgg aatctttgaa aatacgttcc ccagtagctc agtcggtaga gcggctggct
     4981 gttaaccagc atgtccgtgg ttcgagtccg cgctggggag cca
//
LOCUS       DAOOLB010000125         5006 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_20187_length_5006_cov_41.641689, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000125 DAOOLB010000000
VERSION     DAOOLB010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5006
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20187_length_5006_cov_41.641689"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="PLF77_10390"
     CDS             complement(<1..476)
                     /locus_tag="PLF77_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPC09305.1"
                     /translation="MGKLFGTDGIRGEANRHPMTPQIAFGVGQAIAHVFKKGGHRSVI
                     IIGKDTRLSGYMLESSLEAGITSMGGYSYLVGVMPTPGIAFVTQSMRADAGVVISASH
                     NPFQDNGLKIFGGDGYKLTDEKEDIIEDLILNDKLSEFLSKGDDIGRAFRLEGVNGR"
     gene            complement(524..1789)
                     /locus_tag="PLF77_10395"
     CDS             complement(524..1789)
                     /locus_tag="PLF77_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
                     /protein_id="HPC09306.1"
                     /translation="MDLKPRSLKKINDLLERGVSIPNPETIDIGDDVAIDRISGKGVK
                     IYPGCRIYGKETTISSGCQLGYEAPVTIENCQLAEKVELKGGFFSKSVFLEKSNMGMG
                     AHVREGCILEEEAGGAHCVGLKQTILFPFVTLGSLINFCDCLMGGGTSRTNHSEVGSS
                     YIHFNFTPDADKTTPSLLGDVPRGVMLNQPPIFLGGQGGIIGPVRLGFGNVVAAGSVL
                     RHDYPGDNQLIFEKAPACSVKPFYPAAYPHLERIVENNILYLANLKALEAWYTHVRKP
                     FLETGEYGSFLYAGVMEQLSSARKERLKRLKAMAQKAAAAAEEEKNPERTCARGRKEL
                     HEKMGHIEDLCNGKASPASGKTDELRDTFLNDLESIAGKNKTNYIETIQALPAGISSK
                     GVMWLDGIVRDLGENVSRAVPKLGLLKTF"
     gene            complement(1839..3194)
                     /gene="gdhA"
                     /locus_tag="PLF77_10400"
     CDS             complement(1839..3194)
                     /gene="gdhA"
                     /locus_tag="PLF77_10400"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPC09307.1"
                     /translation="MSVIQDVIAKVKQTDPNQPEFHQAVEEVMETLEPTVKKHPEFVK
                     ANLYERIVEPERAVIFRVPWVDDKGNVQVNRGFRVQFNNAIGPFKGGIRFHPSVNLGI
                     IKFLGFEQIFKNALTTLPMGGAKGGSDFDPKGKSDGEVMRFCQAFMRELYRHIGADVD
                     VPAGDIGVGGREIGFMYGYYKKIRNEHTGVLTGKGMSYGGSLIRPEATGYGCVYFAAE
                     MLATRKMDFKGKTVAVSGAGNVAQYAVGKVNQLGGKVISLCDSSSCIIDEAGIDDKKC
                     SFVLDLKNVKRGRISEYANKFKSECFEGKNVWDVIREQGIKVDIALPCATQNEINGKN
                     AAALVKNGCLCVAEGANMPSTPEAIKIFQDKKILYGPGKAANAGGVATSGLEMSQNSL
                     RLLWTREEVDNRLLIIMRSIHKNCYDTAETYGKKGDYVTGANIAGFTKVANAMLAYGV
                     V"
     gene            complement(3260..4261)
                     /locus_tag="PLF77_10405"
     CDS             complement(3260..4261)
                     /locus_tag="PLF77_10405"
                     /inference="COORDINATES: protein motif:HMM:NF014097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrpR regulatory domain-containing protein"
                     /protein_id="HPC09308.1"
                     /translation="MKHLFNPEDIERKVLLILKILNENSESAGARVIARKMKEHGVHL
                     SERTVRYHLKSMDERGLTRLIGRRDGRIITDMGMEEINNARVRDKISLSISRIDVLSL
                     KTTFDIKKRRGLVPVNISLFPQKQFRKALAVMKPVFKKKIAVSDLVAVASAGETLGEV
                     TIPEGKIGFATVCSILINGVLLKHGIPMDSKFGGILQVRHGNPLRFVELINYSGSSLD
                     PSEIFIRGKMTSVKDVVKKGEGKILANFREIPALALSLVENIVADFKKIGVNGALSIG
                     NVGNPVCQTNVDINKVGMILLGGLNPIAAAGEEGFDIENKAMSTVMEYETLQRVEDI"
     gene            complement(4690..>5006)
                     /locus_tag="PLF77_10410"
     CDS             complement(4690..>5006)
                     /locus_tag="PLF77_10410"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPC09309.1"
                     /translation="AFKMPSKKKSKSEIVMNVKATPYAAVEEIMEKKLEDIATILSAS
                     GDRKSKLYEEVMNIVEKGLFKIALRRSNGVKTSAAIFLGINRNTFTDKMTKLGIDCKK
                     SK"
BASE COUNT         1133 a         1263 c         1180 g         1430 t
ORIGIN      
        1 cgtccgttga cgccttccag gcggaaggcc cggccgatat cgtcgccttt ggaaaggaat
       61 tccgaaagct tgtcgtttag gatcagatct tcgatgatgt cttctttttc gtcggtcagt
      121 ttatagccgt ctcctccgaa tattttcagt ccattgtcct gaaaaggatt atgggaggcg
      181 gaaatgacca cacccgcatc ggcccgcata ctctgtgtga caaaggcgat tcccggtgtc
      241 ggcatgacgc ccaccagata ggaataccct cccatcgagg taatacctgc ctccagggag
      301 ctttccagca tataccccga aagcctggtg tctttcccga tgatgataac agaacggtgt
      361 cctcccttct taaatacatg cgcgatagcc tgtcccactc caaaagcaat ttgcggtgtc
      421 atgggatgcc gattagcttc tccacgtatc ccgtctgtcc cgaataattt acccattgtt
      481 ctactcctta tctttagata tctcgcgtcg aacaaattga ttgttaaaat gtttttaata
      541 aacccagctt gggtacggct ctggatacgt tttcaccaag atctcgtacg atgccgtcca
      601 gccacatgac gcctttcgat gaaattccgg ctggcagcgc ctggattgtt tcaatataat
      661 ttgttttgtt tttccccgca atgctttcca gatcgttgag aaacgtgtca cgcagctcgt
      721 cggtttttcc cgaggcgggg gacgctttcc cgttgcagag gtcttcaata tgtcccatct
      781 tttcatgaag ctcttttctg ccccgcgcac aggttcgttc aggatttttt tcttcttctg
      841 ctgcggcggc ggctttttgg gccatagcct ttaatcgttt taatcgttcc tttcttgctg
      901 aagaaagttg ctccatcacc ccggcgtata aaaaggaacc gtattcgccg gtttccagaa
      961 aaggtttcct cacatgcgtg taccaggctt caagcgcttt caaatttgca aggtagagga
     1021 tattgttttc cacaatccgt tccaggtggg gataggccgc cgggtaaaac ggtttcacac
     1081 tgcaggccgg cgctttctca aagatgagtt gattgtctcc ggggtagtcg tgccgcagga
     1141 cggaaccggc ggcgacgaca tttccgaatc caagtctcac cggaccgatg attccgccct
     1201 ggccacccag aaaaatcggc ggttgattga gcatgacgcc ccgcggaacg tcgcccagca
     1261 gagaaggcgt tgttttatcg gcatccggtg taaaattaaa atgaatgtag gaactgccca
     1321 cttctgaatg attggttcgg ctggtgccgc cgcccatcag gcaatcgcag aagttgatca
     1381 gactgcccag cgttacgaac gggaaaagaa tggtctgctt caagccgaca caatgcgcac
     1441 caccggcttc ttcttccaga atgcagcctt cccggacgtg cgcgcccatt cccatatttg
     1501 atttttccag aaagacggat ttgctgaaga accctccctt taattcaacc ttttcggcca
     1561 gttgacagtt ttcaatggta acgggcgctt cgtatcccag ttgacagccg gatgatatgg
     1621 tcgtttcctt tccgtagatc cggcaaccgg gatatatctt gaccccctta cccgaaatcc
     1681 ggtcgatagc gacgtcatcg ccgatatcga tggtttcggg attgggaatg ctcacaccgc
     1741 gttcgagcag atcatttatt tttttgagcg atctcggttt taaatccata cggtctccct
     1801 gacgttaagg acggaagatg cctcagcatc cgcgttcgtt aaaccacgcc gtaagccagc
     1861 atggcattgg cgactttggt aaatccggca atattggccc cggtcacata gtcgcccttc
     1921 ttcccgtagg tttctgccgt gtcgtagcaa tttttgtgaa tgctcctcat aatgatgagc
     1981 aggcggttgt cgacttcttc acgcgtccat aacagcctga ggctgttctg gctcatttcc
     2041 aggccggacg tggcgacacc gcccgcattg gccgcttttc cgggaccgta aaggattttt
     2101 ttatcctgga aaattttgat ggcttcaggc gttgagggca tgttggctcc ttcggcaaca
     2161 cagagacagc cgtttttcac cagtgccgcc gcgttcttac cgttgatttc attttgcgtg
     2221 gcgcagggca gggcgatgtc cactttgatg ccctgttcgc gaatgacgtc ccagacattt
     2281 ttgccttcga agcattccga tttgaatttg tttgcgtatt cggaaatgcg cccgcgtttg
     2341 acgtttttca gatcaagcac gaagctgcat ttcttgtcat caatgccggc ttcatcaata
     2401 atacacgacg aagaatcaca cagagagatg accttgccgc ccagttggtt gacttttcca
     2461 accgcatact gagccacgtt gccggcgccg ctgacggcga cggtttttcc tttgaaatcc
     2521 attttgcggg tggccagcat ttccgcggca aaatacacac agccgtaacc cgtcgcttcg
     2581 ggacgaatca gacttccgcc gtatgacatt cctttgccgg tcagcacgcc ggtgtgctcg
     2641 ttgcggattt ttttgtaata gccgtacata aagccgattt cacgaccgcc gactccgatg
     2701 tctcctgcgg gtacgtccac gtcggcgccg atgtgacggt acaattcgcg cataaatgcc
     2761 tgacagaaac gcatgacttc tccgtcggat tttcctttgg ggtcgaaatc ggaaccgcct
     2821 ttggcgccac ccatgggcag ggtggtcagt gcatttttga aaatctgttc aaaccccaga
     2881 aatttgatga tgcccagatt cacggacgga tgaaaacgga taccgccttt gaaggggccg
     2941 atggcgttgt tgaattgcac gcggaatccg cgattgacct gaacgtttcc cttgtcgtcc
     3001 acccagggaa cccggaaaat gaccgctctt tccggctcga caatcctttc gtaaaggttg
     3061 gctttgacga actcgggatg ctttttgacg gttggttcca gcgtttccat aacttcttca
     3121 acagcctggt gaaattccgg ctgattggga tcagtctgct tgactttggc gatcacatct
     3181 tgaataactg acataaatta tcatcctcct aaaaaattgt tattgatttc attttttgac
     3241 gttcatgatg caacgttttt catatatctt caacccgctg aagcgtttca tactccatga
     3301 cggtggacat cgctttattt tctatgtcga acccttcttc gccggctgcg gcaatcgggt
     3361 tgagtcctcc aagaagaatc atgcccactt tgttgatatc cacattcgtc tggcaaaccg
     3421 gatttccaac attaccgatc gaaagggcgc cgttaacgcc gatcttttta aaatcagcaa
     3481 cgatgttttc caccagagag agcgccaatg ccgggatttc tcgaaaattt gccagaatct
     3541 ttccttcccc ctttttgaca acgtccttga cggaggtcat tttgccgcgg ataaaaattt
     3601 cggacggatc aagcgacgag ccggaataat tgatgagctc cacgaaacgc aaaggattac
     3661 cgtgccgcac ctgcaagatg ccgccgaatt tggaatccat cggaatgccg tgcttcagaa
     3721 gaacgccgtt aatgagaatg ctgcaaacgg tggcaaatcc gattttcccc tcgggaatgg
     3781 tgacttctcc cagcgtttca ccggcagacg caacagctac aagatccgac acggcaattt
     3841 tctttttgaa aaccggtttc atgacggcca aggccttgcg gaattgtttt tgagggaaca
     3901 gagaaatatt aacgggaaca aggccgcgcc gtttttttat gtcgaaagtc gtttttaagg
     3961 aaagaacatc aatccgggaa atggaaagac tgattttgtc ccggacgcgg gcattgttga
     4021 tttcttccat ccccatgtct gtgatgattc tgccgtcacg ccgcccgatg agcctggtca
     4081 agcctctttc atccatcgac ttcaagtgat agcggacagt tctttcgctt aaatgaacgc
     4141 cgtgttcttt catttttcgc gcgatgacgc gggctcctgc ggattctgaa ttttcgttga
     4201 gaatcttgag aatcagaagg acttttcttt ctatgtcttc gggattgaat aaatgtttca
     4261 tggctaatcg gcaaccgttt gccgtatatc tactttcaaa tgatgtcctt gtcaataaaa
     4321 aataatattt ttttaaacgg attttcacag gaacccgatg cgcagcatat caaccgtaac
     4381 aaatggttat tttctgatat attgttcaga atttcaatat gttgtgtggt aattattgct
     4441 atgaaagaag tgggtttgtg gggtttgtgt ttcttcggtc cgacgtgatg tcgtccggac
     4501 cgggagtaca atttcgtccc tatctggtca agatgtgatc ctgtgccatt actttcgttg
     4561 agctccaact attctgtcgt atgatgacgg gctacttttt gtgttttgag gtggttaaaa
     4621 aaagcgtccg tcaaaagatg aacgtgtttc ggtatgtttt aatcggggcg ggaaagcaca
     4681 ttgcagattt tacttggact ttttacaatc aatacccagt ttggtcatct tatctgtaaa
     4741 ggtgttgcgg ttaatgccca ggaaaattgc cgcggaagtt ttaacgccgt ttgaccggcg
     4801 taaagcaatc ttaaacaatc ccttttccac gatattcatg acctcttcat aaagcttgct
     4861 tttgcgatca ccggacgcag acaaaatggt ggcaatatcc tcaagctttt tttccataat
     4921 ttcttcaacc gcggcataag gcgtggcctt aacgttcatg acaatttcag atttgctctt
     4981 tttcttcgac ggcattttaa acgcat
//
LOCUS       DAOOLB010000126         4803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_21313_length_4803_cov_29.355729, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000126 DAOOLB010000000
VERSION     DAOOLB010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21313_length_4803_cov_29.355729"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            303..3221
                     /locus_tag="PLF77_10415"
     CDS             303..3221
                     /locus_tag="PLF77_10415"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HPC09310.1"
                     /translation="MPAPQIILELIERFERNIESYKSNGYNETQVRREFIDPFFEALG
                     WDVNNTAGHAEAYKDVIHEDAVKVSGVTKAPDYSFRIGGQRKFFVEAKKPSVNLKDAP
                     AAAFQVRRYAWSAKLPLSIVTDFEEFAVYDCRIKPDQKDKSDKARITYYSWRQYADKW
                     DEIAAVFSKDVILKGSFDKYAESSKAKRGTSEVDNAFLAEIESWRDALARNIALRNTL
                     SVRELNDAVQRTIDRIIFLRIAEDRGIEDYGSLRSLCNGANIYGRLQELFYKADDRYN
                     SGLFHFRNEKDQAEPPDNLTTKLTIDDKPLKEILTRLYYPDSPYEFSVLPADILGQVY
                     EQFLGKVIRLTSDHRAVVEDKPEVKKAGGVFYTPTYIVDYIVKNTVGKLLEGKNPKTA
                     EKLRILDPACGSGSFLLGAYQCLLDWHLQWYTENDPEKHAKAKRPILYKGYGDTWHLT
                     TAERKRILLNNIYGVDIDTQAVEVTKLSLLLKVLEDETSDTLNRQRVLFHERALPNLG
                     NNIKCGNSLIGPDFYDGKQMNMLDSEEAQRVNVFDWQAEFPEIFKAGGFDAVIGNPPY
                     GALFNNEDKAYLGRKFESVVGKYDSYGFFIEKGILLLQKEGCLGFITPHTWLTVIEAE
                     ALRKYLLTNATIESLVRLPTRVFSNATVNTILAFVKKGKKLNNNKQCIRVLLLPRDIA
                     ISEIKESMGEVRHFTESSWATSEAHMFNIEVGTKEQKLVNKIKQAGVLLTELCDFCVG
                     VQAYDSHTGQERSIIDLRIYHSTTKRDQTFRKELNGNDVSRYQIQWTEGNWISYGPWL
                     AHPRQEYFFKGPRILVREITGSGRYRIHAAFTDEDFVNYKTILNVIIKQESRSAGYHD
                     FFILGLLNSELLSWYFPRSSNKLVTKTFPRISILDMKRFTAPLINFSNKAEKKRHDNM
                     VRLVEQMLALNKQLAEAKTGHEQTLIQRQIDATDKQIDKLVYELYGLTEEEIAIVESK
                     "
     gene            3467..3895
                     /locus_tag="PLF77_10420"
     CDS             3467..3895
                     /locus_tag="PLF77_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09311.1"
                     /translation="MPKYKYKQKLKEYIARHGKPDALTEYFILQGFSEDEIKEGLEPY
                     IEEWEEAIYWIGRDDGSEYDWDLWKRSSLYEVLRHATTEQIEPYRERIEKADEKFRSM
                     TYEVKDSYGDLLDKNETINRDMHWWLFRIPIHGQVWRVCR"
     gene            complement(4008..4268)
                     /locus_tag="PLF77_10425"
     CDS             complement(4008..4268)
                     /locus_tag="PLF77_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09312.1"
                     /translation="MKLDLFFASLIGAAVGCAVAYGIIRLKGIWILGALLYIAGGIMA
                     FLFAHIIFWHLVADTHYVFIPGTVGFCIWSVFIARLFFPRHV"
     gene            4475..4606
                     /locus_tag="PLF77_10430"
     CDS             4475..4606
                     /locus_tag="PLF77_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09313.1"
                     /translation="MKNRLDMSNFQGRFNFADKQIDKLVYELYGLTEEEITIIEGGK"
BASE COUNT         1442 a         1103 c         1131 g         1127 t
ORIGIN      
        1 atacgattcg tgaacctttt gcttactttg aagttccgat ggaagaagat gttgtggtaa
       61 aaatctttgc cggctgtccc cccactggtg gtgacgcagt ttaacaatct ctttatgtta
      121 aaataaaaaa ggcagaatca taaacgattc tgcctttttt tgttagtatg acgatgtccg
      181 caatcctttg tcgtccgttt atatcaacct tcaggaaggg gatcggggtt gacagtttta
      241 ctttcagaga gtataaaacc acttcaatca aaataatatc caatgttaac aagggacctc
      301 tgatgcctgc accgcagatc attctggaac taattgaacg attcgaacgc aacattgaat
      361 cctataaatc caacgggtat aacgaaacgc aggttcgccg cgaattcatc gatcccttct
      421 ttgaagcact gggctgggat gtcaacaata cggcaggaca tgccgaagcc tacaaggatg
      481 tcatccacga agacgccgtc aaagtcagcg gcgtcaccaa agcgcccgac tattcctttc
      541 gcatcggcgg acagcgcaaa ttctttgtcg aagccaagaa accatccgtc aacctcaagg
      601 acgcgcccgc cgccgccttt caggtaagac gttacgcctg gtcggccaaa ctgccgcttt
      661 ccatcgtgac cgactttgaa gaattcgccg tttacgactg ccgcatcaag ccggatcaaa
      721 aagataaatc cgacaaagcc cggatcactt actactcctg gcgccagtac gccgacaagt
      781 gggacgaaat tgccgccgtc ttttccaagg atgtcattct caaaggctct ttcgataaat
      841 atgccgaatc cagcaaggcc aagcgcggca cgtccgaagt ggataacgct tttcttgccg
      901 aaatcgaaag ctggcgcgac gcgcttgccc gcaacatcgc cctgcgcaac acgctgagtg
      961 ttcgagaact caacgatgcc gtccagcgca ccatcgaccg cattatcttc ctgcgcatcg
     1021 ctgaagaccg gggcattgaa gactacggca gcttgcgctc tctttgcaac ggcgcgaata
     1081 tttacggccg tctgcaggag cttttctata aggccgacga ccgctacaac tccggcctgt
     1141 ttcatttccg caacgaaaaa gaccaggccg aaccgcccga taatctgacg acgaaactca
     1201 ccatcgatga caaaccgctc aaagaaattc ttacgcgcct ttactatccc gattctcctt
     1261 atgagttttc ggttttgccc gccgatatat tggggcaggt ctatgaacag tttttaggaa
     1321 aggtcattcg cctgaccagc gatcaccgcg ccgtggtcga ggacaagccc gaagtcaaaa
     1381 aagcgggtgg cgttttctac acgccgacgt atatcgttga ttacatcgtc aaaaatactg
     1441 tgggtaaatt gttggaaggg aaaaatccaa aaacagcaga aaagctccgc atccttgatc
     1501 ccgcctgcgg ctccggctcg tttttgctgg gcgcgtatca gtgtctgctc gactggcatt
     1561 tgcagtggta caccgaaaac gatcccgaaa aacacgccaa agccaaaagg ccgatactct
     1621 ataaaggata cggcgacacg tggcacctga ccaccgccga acgcaaacgg attctgctca
     1681 ataacattta cggcgtggat attgacacgc aggccgtcga agttaccaag ctttcgctgc
     1741 tcttaaaggt gctggaagat gaaacctccg atacgcttaa ccgccagcgg gttcttttcc
     1801 atgagcgcgc gctgccgaat ttgggcaaca acatcaagtg cggcaattcg ctcattgggc
     1861 cggatttcta cgacggcaaa cagatgaaca tgctggattc cgaagaagcc cagcgcgtca
     1921 atgtgtttga ctggcaggct gagtttcccg aaatcttcaa agccggcgga ttcgacgccg
     1981 tcatcggcaa cccgccgtat ggggctctat tcaataatga agataaagca tatcttggtc
     2041 gtaagtttga gtcagttgtc ggcaagtatg actcatatgg tttcttcatc gaaaaaggaa
     2101 ttttgttgct tcaaaaagag ggttgcctgg gattcattac tccacacacg tggcttactg
     2161 tgattgaagc agaggcgctg cgaaaatatc ttctgactaa tgctacaatt gaaagtttgg
     2221 ttagactacc gactcgcgtg ttcagtaatg ccaccgtcaa cacgatttta gcgtttgtaa
     2281 aaaaagggaa gaaactaaac aataataaac agtgcatcag ggttcttctt ctgcctcgtg
     2341 acattgcaat ctcagaaatt aaagaaagta tgggagaggt tcgccatttt accgaaagtt
     2401 cgtgggcaac tagcgaggct cacatgttta acatagaagt tggtactaaa gagcagaaac
     2461 tagtcaataa gataaagcag gccggagtgc tattgacaga gctttgtgat ttttgtgtag
     2521 gtgtccaagc atatgattct cacacgggac aagaaagaag cattattgat ttacgtatat
     2581 atcattcaac aacaaaaaga gatcaaacat ttcgtaaaga gcttaatgga aatgatgtct
     2641 cccgttatca aatccagtgg actgagggga actggatatc ttatggtcca tggcttgctc
     2701 atcctcggca agagtatttc tttaaggggc ctcgcattct tgttcgtgaa attacaggtt
     2761 ctggacgtta cagaatacat gcggcattca cggacgaaga ttttgttaac tataagacaa
     2821 tcctgaatgt gattattaaa caagagagtc gcagtgcagg ttaccacgac ttttttatcc
     2881 ttgggttgct gaattcagaa ctactttcat ggtactttcc tagatcaagt aacaagttgg
     2941 tcactaaaac atttccacgc atttcaatac tcgatatgaa gcgattcacg gctccactaa
     3001 ttaatttctc taacaaggca gaaaaaaaac gtcacgacaa catggtgcga ctcgttgagc
     3061 aaatgcttgc cctgaacaaa caactggccg aagccaaaac cggtcacgaa caaacattaa
     3121 tccagcgcca gattgacgcg accgacaaac aaattgataa attagtttac gaactctacg
     3181 gcctgacgga agaagaaatt gctattgtgg aaagcaaatg aagaatcatc ggacgcaaga
     3241 agtcccacgg cgctccgcgc ctgggatagt tccacttacg tttcgagcag cactacgctt
     3301 gctctcaacg agtgtcacta aaataaagat agtcgaaggg aaataaaatt tggggcgtgc
     3361 tcaggggagc acgccctaca aaaaaatgga aacacaattt tgaaatgtag ggattgattc
     3421 ccgatcaatc cgtaaaaact acgatgtttc taagggattg ttaccaatgc ccaagtacaa
     3481 atataaacaa aagctaaaag aatatattgc caggcacgga aaacctgatg cgctgacgga
     3541 atacttcatc cttcaaggtt tctcggaaga tgaaataaaa gaggggctgg agccgtatat
     3601 tgaggaatgg gaagaagcca tctattggat tggaagagac gatggatcgg aatatgattg
     3661 ggatctgtgg aaacgcagct cattgtatga ggtattacgc cacgccacaa ccgagcaaat
     3721 tgagccttat cgggaaagaa tcgaaaaggc tgacgaaaaa ttcaggtcaa tgacatatga
     3781 agtcaaagat tcttatggcg atctgcttga taagaatgag accataaaca gagatatgca
     3841 ttggtggctg ttcaggatac caatacatgg ccaagtatgg agggtttgcc gataattatg
     3901 agcaagatag gagaataaaa aacggcatgc ggtggggcct cccacaaacc ccactttacg
     3961 cgcccccgct ggaagcgggg tgctacggcc gggagaatat taccgtgtta cacatgcctg
     4021 ggaaaaaata agcgagctat aaaaaccgac cagatgcaga agccaactgt gccggggata
     4081 aaaacataat gtgtatcggc aaccagatgc caaaaaataa tatgcgcgaa gagaaaagcc
     4141 attattccgc cggcgatata aagcagcgcg ccgagaatcc agatgccctt gagccggatg
     4201 attccgtaag cgacggcgca gccgactgcc gcgccgataa gagaggcaaa aaagaggtct
     4261 aatttcatga tcgttgattc cccacgttgt gcaatcgacg tgatgtaagg cgttttgccc
     4321 gtgtccctaa ttcggctaca agtttagaca atgcatccaa agaagtcaag aacgactttg
     4381 gtaacacgag cagacaggac tctgctgagt ttaagtttca gactgcactt gttaacaaga
     4441 tgtccgaaac gatcatggag accgaaaaaa aatcatgaaa aatcggcttg acatgtcgaa
     4501 ttttcaaggt aggttcaatt ttgccgacaa acaaatcgac aaactggtct acgaactcta
     4561 cggcctgacg gaagaagaaa ttacgataat tgaaggcggt aaataaatat ggattcccgc
     4621 caaaaccatg cgggaatgac agtgcggggt taattgttac ctacccaagt aaatgaaagt
     4681 gtagggattg atccaccgat caatccgcaa aaaaacggcg cgctctggag ttgcgccctg
     4741 aaatttatgg atgagattta tgacgatcta taaacggttt gtgggaacgt ggaaacttaa
     4801 atc
//
LOCUS       DAOOLB010000127         4631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_22365_length_4631_cov_30.352928, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000127 DAOOLB010000000
VERSION     DAOOLB010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22365_length_4631_cov_30.352928"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PLF77_10435"
     CDS             <1..213
                     /locus_tag="PLF77_10435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HPC09314.1"
                     /translation="AMKYGARNQIVGKVESIKKGAIMCEVKVKITGGECMESVMTTNS
                     LKELGIKKGDKVKVVVKAVNVLLVKE"
     gene            complement(307..777)
                     /locus_tag="PLF77_10440"
     CDS             complement(307..777)
                     /locus_tag="PLF77_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09315.1"
                     /translation="MLKLDIPGSTIMELQFLVLDYNGTIACDGRLIPGVMDRITDLAK
                     SLSVHILTADTFGSVRTELNGIPCETAIIPKGKQAEAKADYIRQLGAEHCVCVGNGRN
                     DALMLKEAKLGIAVIQTEGAALEALLAAHVLTSGILDALDLLRHPQRLAATLRS"
     gene            967..1806
                     /locus_tag="PLF77_10445"
     CDS             967..1806
                     /locus_tag="PLF77_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HPC09316.1"
                     /translation="MILRKYRWFVTVLLMISALVWTSAAMAQSLDVFKGEKGDLRISG
                     GTAHIPVMKEAAKRIMTVNPAIHISIAGGGSGVGIKQVGEGLVDIGNTGRTPTDDEVS
                     KYGLKMFKWAVDGVGVIVHKKNSVRNLKKSDLIKIYSGEMTNWKELGGSDNPINIYTR
                     DEASGTREVFWEKGLGKGAISAKAKVVVSNGAMKSGIATDPNGIGYTSVGHIDDTVAT
                     VTLDGVAPTIENVKSGSYKISRGLFSNTKGDPTSLTKKFIDYLFSPEGQKIVVDEGFI
                     AVK"
     gene            1803..2666
                     /locus_tag="PLF77_10450"
     CDS             1803..2666
                     /locus_tag="PLF77_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPC09317.1"
                     /translation="MIKRFGFWMEKGFLAAALWSMFLTILIFAFMLVLGLPLFKEGRL
                     LSLLTSPWAPGEGQFGIYPMIISTTVISFLSLCLGAPLALGVSVLICLIQPGRLGRWL
                     KKVVVLMTAVPTVVYAFVGVFLLVPLVQSVFSAGSGMNILTASFVLALLIAPTMILIF
                     SNSFNNVPLAHQYAADALGASSAQKLFYVILPASWRGIVAGLILGAGRAMGDTLVALM
                     LAGNAVAVPGSIFDSARTLTAHIALVTAADFDSVEFRTIFACGILLYLMTLIMVLAVR
                     FLSSLKKEADL"
     gene            2663..3544
                     /locus_tag="PLF77_10455"
     CDS             2663..3544
                     /locus_tag="PLF77_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPC09318.1"
                     /translation="MNRFVEIGVALFAWLCAFVLLAAVGTVFVFLLIKGGAALNVQTI
                     FGDTPVWEAITLKKAVFNGLFPAIAGTLLLVVMAVGLALPVGIAAGIYLAQYSGVRMK
                     SIFSLFCDILAGVPSILVGLFGFSLSIFLHKYVSTEIYPCLLISAASLSFLILPYLIR
                     TTQIALEQIPPEERLTALALGADKLQNIIYVLIPRSLSGIISGIILSIGRCAEDTAVI
                     MLTGVVAMAGVPQSLLAKYEALPFFIYYISAQYTDMEELRMGYAAAIILVVICAVLFL
                     AAFYIKNRLTEKALYSV"
     gene            3554..4267
                     /locus_tag="PLF77_10460"
     CDS             3554..4267
                     /locus_tag="PLF77_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC09319.1"
                     /translation="MNPREKIKIQVDNISFFYGRRKILQDVSAQVAASSITAVIGPSG
                     QGKSTFLMIMNRLWEQIPETRMEGRVHMLLKNKMTDIYAGDVSLPDLRRSVGMVFQKP
                     NPLPMSIYRNVAFPLKLAGVKDEALIRRRVETALKEAFLWDEVKDRLEHDARDMSGGQ
                     QQRLCIARALIVGPEVLLMDEPTSSLDHKAASIIEELIVKLKTRCTILLVSHLMEQVS
                     RLADTVMELDEGKRITRQN"
BASE COUNT         1090 a         1083 c         1249 g         1209 t
ORIGIN      
        1 gccatgaagt acggtgcaag aaatcaaatt gtaggaaaag tggaaagcat caaaaaaggc
       61 gcgatcatgt gtgaagtaaa ggttaagatt acgggcggcg agtgcatgga atccgtcatg
      121 acaaccaact ctctgaaaga actgggtatt aaaaaaggcg acaaggttaa agtggtcgtc
      181 aaggccgtca atgtgctttt ggtcaaggag taaaattttg gcagaatagt tgatctgcaa
      241 gacgccgcaa cattgcaaag ccctgaaagc aatgttgcgg cgtctcatta ttttatttgt
      301 ttcttgttat gagcgaagcg ttgcagcgag tctctgagga tggcgcaaaa ggtcgagcgc
      361 gtccagaata ccagacgtta aaacatgcgc ggcaagcagc gcttccaaag ctgccccttc
      421 ggtctggatg acggcaattc cgagttttgc ttccttgagc atcagcgcgt cattacgacc
      481 gttgcccacg cagacacaat gttccgcgcc cagttgccgg atataatccg ctttggcttc
      541 ggcttgtttt ccctttggaa tgatggcggt ttcacaggga atgccgttca gttctgtccg
      601 gacactgccg aaggtgtcgg ctgtcagaat gtggacggaa agacttttag caaggtcggt
      661 tatcctatcc atgacaccgg ggatgagacg gccgtcacag gcaatggtgc cgttataatc
      721 cagaacaaga aattgcagtt ccatgattgt gctgcccgga atatccagtt tcaacatctg
      781 ccttcatctc ctttcagatt cggtttaaga ctaaactata aagcgatgta aatccactat
      841 gaataatagt tatatagaca gaagcattat aactattatg aatttggcaa tcaacacgcc
      901 aggtgttagt taagcgttgc ctgaccgtgt cgggcaggcc aacaaaatat ttataaggag
      961 agctacatga ttttaagaaa gtatcgatgg tttgttacgg ttttattgat gatcagcgca
     1021 ctggtttgga cctccgctgc catggcgcag agtttggatg tttttaaagg cgaaaagggt
     1081 gatctccgga tatcgggcgg tacggcgcat attccggtca tgaaggaagc agccaaaaga
     1141 attatgacgg tgaatccggc tattcacatt agcatcgccg gcggcggttc cggtgtcggg
     1201 atcaagcagg tgggcgaggg actggtcgat atcgggaata ccggtcgcac accgacggac
     1261 gatgaggtgt ccaagtatgg tctgaaaatg tttaagtggg ctgttgacgg cgtgggcgtg
     1321 atcgtacaca aaaagaacag tgttaggaat ttaaaaaaga gtgatctgat taaaatttat
     1381 tccggcgaga tgaccaactg gaaggaattg ggcggttctg acaatcccat caatatctat
     1441 acccgtgatg aagccagtgg tacccgcgaa gtgttctggg aaaagggact gggcaaaggc
     1501 gccattagcg cgaaagcgaa ggtggttgtt tccaacggcg cgatgaagtc cggcattgcc
     1561 acggatccca acggtatcgg ctacacgtct gtcggccata ttgacgacac ggttgcgacg
     1621 gtcacgctgg acggcgtcgc gcccacgatt gaaaatgtca agagtggcag ctataaaata
     1681 tctcgcggcc ttttcagcaa caccaaaggc gatccgacaa gcctgacgaa aaaatttatt
     1741 gattatctgt tcagtccgga agggcagaag attgttgtcg atgaagggtt cattgcggtg
     1801 aaatgatcaa acggtttggt ttctggatgg aaaaggggtt cctggcggca gcgctttgga
     1861 gcatgtttct gaccatcctg atctttgctt tcatgctggt tctcggcctt cctctgttta
     1921 aggaaggccg tttgctttcc cttctcacgt cgccctgggc gccgggcgaa ggtcagttcg
     1981 gcatttatcc gatgatcatt tccacaacgg tgatttcttt tttaagcctt tgcctgggcg
     2041 caccgctcgc gctcggtgtg tctgtcttaa tttgtctgat tcaacccggc cgtcttgggc
     2101 gatggctgaa aaaagtggtt gttctgatga cggctgttcc gacggttgtc tatgcgtttg
     2161 tcggcgtttt cctgctggta ccgctggtgc agtctgtatt ttcggcggga tcgggtatga
     2221 acattctgac ggcgtcgttt gttctcgctt tgctgatcgc gccgaccatg attctgattt
     2281 tttccaacag cttcaacaat gtgccgcttg cccatcaata cgccgccgat gcactggggg
     2341 ccagctccgc gcagaaatta ttttacgtca ttctgcccgc gtcctggaga ggcattgtgg
     2401 ccgggcttat tttgggtgcg gggcgcgcca tgggcgacac gctggtggcg ttaatgctgg
     2461 ccggcaatgc cgtggctgtt cccggctcta ttttcgattc ggcgcggacg ctgaccgctc
     2521 acatcgcgct ggtgacggcg gcggattttg actccgtcga attccggaca atttttgcct
     2581 gcggaattct gctttacctg atgacgctga tcatggtttt agccgttcgg tttctttcct
     2641 cattaaaaaa agaggcggat ttatgaaccg attcgtggaa ataggcgttg cgctttttgc
     2701 ttggctgtgc gcgttcgttc tccttgcggc cgtgggaacg gttttcgttt ttttactgat
     2761 caagggcggc gccgcgctga atgtgcaaac cattttcggc gacacgcccg tctgggaggc
     2821 catcaccctg aaaaaggccg tgttcaacgg tctttttccg gcgatcgccg gcacgttgct
     2881 cctggttgtg atggccgtcg gactggcgct gcccgtcggc attgcagccg gaatttatct
     2941 tgcgcaatac agcggagtgc gcatgaaaag cattttcagc cttttttgcg atattctggc
     3001 gggcgtgccc tccattctcg tggggctttt cggcttctca ctatccatat ttctgcataa
     3061 atatgtatca acagaaattt atccctgcct gctgatttca gccgcgtcgc tttcttttct
     3121 gatactgccc tatctgatcc ggacgacgca aatcgcgctg gaacagattc caccggaaga
     3181 acggctgacg gcgctcgcgc ttggagcgga caagctgcag aacattattt atgttctgat
     3241 tccccgttcc ctttcaggca ttatcagcgg catcattcta tccattggcc ggtgcgcgga
     3301 agacacggcg gtcatcatgc tgaccggcgt cgtggccatg gccggtgtcc cgcagtcgct
     3361 tctggccaag tacgaagccc tgcctttttt tatttattat atttccgcgc aatataccga
     3421 catggaggaa ctgcggatgg gttatgcggc ggcgattatt ctggtagtga tttgcgccgt
     3481 tttatttctt gccgcttttt atattaagaa ccggttgacg gaaaaagcat tatacagcgt
     3541 ataaggttga tatatgaacc cacgcgaaaa aataaaaatt caggtagaca atatttcatt
     3601 tttctacgga cggcgaaaga ttcttcagga cgtttccgcg caggttgccg catcatccat
     3661 cacggcggtc atcgggccgt ccggccaggg caagtccacc tttctgatga tcatgaaccg
     3721 cctttgggag cagattccgg aaacgcgaat ggaagggcgt gtgcacatgc ttttgaaaaa
     3781 caaaatgacg gacatttatg ccggggacgt atcccttccc gacttgcgca gatcggtcgg
     3841 catggttttt caaaaaccca atcctctgcc gatgagcatt taccgaaacg tggcgttccc
     3901 gctgaaactg gccggtgtaa aagatgaagc cctcatccgg cgccgggtgg aaaccgcgct
     3961 gaaagaagca ttcctctggg atgaagtgaa ggacagactc gaacatgatg cgcgggacat
     4021 gtccggcgga cagcagcaga ggctctgcat cgcgcgggcg ctgattgtcg gtccggaggt
     4081 gctcctgatg gacgagccca cgtcttcgct ggatcacaag gccgcctcta ttattgaaga
     4141 actgatcgtc aaacttaaaa cacgctgcac gattttgctg gtatctcatc tgatggagca
     4201 ggtatcccgt ttggcggata ctgtgatgga gcttgatgag ggaaaacgga tcacccggca
     4261 aaattaaatg atggcccctg acatcagtaa aaaataaaca tcattttttt caaacggctg
     4321 tcatggcgcc ataatcgtca catcatgtcc agtcttgatc gtcaatattt ccgcacaccg
     4381 gaaacgcgac gccatgactg attcaaaggc aaaacatatt attcttgcat gtatcgacaa
     4441 tgcgaacaaa gaataattca agacgatata tttcttttag ttatttgaca tattctgttt
     4501 gcttatatat agccatcgca gcatgatagt gaacaggcag tggaaggaga agatatgtct
     4561 gaaaatacag caaacaaaga atgggaaccg aaaatcgccg ccatcgtctg caactggtgt
     4621 acgtacgcgg g
//
LOCUS       DAOOLB010000128         4595 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_22608_length_4595_cov_33.421806, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000128 DAOOLB010000000
VERSION     DAOOLB010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22608_length_4595_cov_33.421806"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="PLF77_10465"
     CDS             <1..821
                     /locus_tag="PLF77_10465"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HPC09320.1"
                     /translation="YPVVVDKIITEVDTKDIKTFRFKFVNPEDEQKYAYIPGQFGELS
                     IFGKGESPIGIASSPTQKGYVEFTVQKAGVVTTALHEMEEGTVMGIRGPLGNSWPLDF
                     LAGKNIVIVGGGFAFTTLRSLINYMLFEDNRKRFGNITVIYGARNPGLLLYKDELVEW
                     GKRSDINLNVTVDKGDATWKGKEGFVPAVCKEVAPSKENAVAVICGPPVMIRFTLPVF
                     FDLGFSKENILTSLEMRMKCGIGKCGRCNVGSKYVCKDGPVFSLAELDKLTPEY"
     gene            978..1814
                     /locus_tag="PLF77_10470"
     CDS             978..1814
                     /locus_tag="PLF77_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPC09321.1"
                     /translation="MNKIIEKENLSESVIRMVLEAPVVAQKRKAGQFIVLKIHEKGER
                     IPLTIVDSDAQKGAITIIFQIVGKTTALLAAMAPGDGIQDVQGPLGNPTEIENFGRVV
                     CVGGGVGVGVVFPLIVALKKAGNDVVSIIGSRTKNLLILEEEIKANSDRLVVTTDDGS
                     YGVHGFVSNVLQEMIDKGEKIDRVFAIGPVPMMRVICDVTKPHGIKTIVSLNSIMVDA
                     TGMCGACRVDVGGKTRFTCVDGPEFDGHQVDFKLLADRLRMYCDQEREAYEKHKCGCD
                     GN"
     gene            1780..3219
                     /gene="gltA"
                     /locus_tag="PLF77_10475"
     CDS             1780..3219
                     /gene="gltA"
                     /locus_tag="PLF77_10475"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPC09322.1"
                     /translation="MKNTSVGVMETKETKPKKEKVPRQAMPEQDPKERIHNFAEVPFG
                     YSEETAIIEAQRCIQCKKPGCVSGCPVDVKIPEFIKAVAEGRFIDAALKLKETNGLPA
                     VCGRVCPQEDQCEKECILGKKGEPVAIGRLERFAADYERNSGKISIPPAVQSNGKKIA
                     VAGAGPAGLTIAGDLVKLGYDVTIFEALHKAGGVLVYGIPEFRLPKAIVEAEVDYLKK
                     MGVKIETNFVIGRTKTIDELFADGFDAVFIGVGAGAPVFMNIPGENLSGIYSANEYLT
                     RSNLMKAYLFPEYDTPIVRGKKVAVIGGGNVAMDSVRTALRLGAERAMIIYRRTEVEM
                     PARKEEVHHAREEGVEFLLLHNPVEYIGNEAGWVTGMKCIKMELGEPDASGRRSPVPI
                     QGSEFVIDVDTVVVAVGTMANPIVPATTTGLETNKKGYILTKDESGLTSREGIYAGGD
                     IVTGSATVILAMGAGRKAANAIHEYLSNK"
     gene            complement(3254..3886)
                     /locus_tag="PLF77_10480"
     CDS             complement(3254..3886)
                     /locus_tag="PLF77_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HPC09323.1"
                     /translation="MKFSLLQNILLGLSLAAPFGPVNVEIVKRGLNSGFKQAFATGLG
                     AMTADATYLTIIFFGLVSFLKMPLMKIFFALAGSLILIYLGVSSVKDFLDKTDYDEKQ
                     NRRFFKNPYVAGYVIALSCPITIVWWTGVFGALLVSQDSIQTNFSAYLSCLTILLGCF
                     IWIVFLSAALHWGRKIVNEKFIGFVSLIAGMCLAGFGLAFLCRSVHLIIH"
     gene            4172..>4595
                     /locus_tag="PLF77_10485"
     CDS             4172..>4595
                     /locus_tag="PLF77_10485"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HPC09324.1"
                     /translation="MAKFEYNEEAVKNLKLRDTSVLDRLIDFESAREAHSKKEHAKKD
                     KRMTIGEAVATFVQDGDIITDGGFSYVRTPHQAFHEVIRQGKKNLQMIGSPNTNQSFF
                     IFYGLVNYSHNSYSGAEMRGIDRLYDRALKNDRVTVLSE"
BASE COUNT         1242 a         1028 c         1226 g         1099 t
ORIGIN      
        1 cgtatcccgt tgtggtggat aaaatcatta ccgaagtcga taccaaagac atcaagacat
       61 tccgttttaa attcgtgaac ccggaagacg aacagaaata cgcctacatt cccggacaat
      121 tcggcgaact ttccatcttc ggaaaaggcg aatcgccgat tggtatagcc tcttctccga
      181 cgcagaaagg gtacgtggag ttcaccgtac agaaagccgg tgtggtgacc acggcactgc
      241 acgagatgga agaaggaacg gtcatgggca tccggggtcc tctgggcaat tcctggccgc
      301 tggacttcct ggcgggcaaa aatatcgtca tcgtcggcgg cggtttcgcc ttcacgacat
      361 tgcgctcatt gattaattac atgctttttg aagacaaccg caaacgcttc ggcaatatca
      421 cggtgattta cggcgcacga aatcccggac tgctgcttta taaggatgaa ctggttgaat
      481 ggggcaaaag atcagacatc aatcttaatg tgaccgttga taaaggcgac gcgacctgga
      541 agggcaagga aggttttgtc cccgcggtat gcaaagaggt cgcgcccagc aaggaaaacg
      601 ccgtggcagt gatctgcgga cctccggtca tgatccgttt caccctgccg gtatttttcg
      661 acctgggctt ttccaaggaa aacatcctca cgtccctgga aatgagaatg aaatgcggta
      721 tcggcaaatg cggacgctgc aacgtgggta gcaaatatgt ctgcaaagac ggtccggtat
      781 tttctctggc ggaactggac aaactgacac cggaatatta aaaaacgttt cagcaaagaa
      841 tctgcgatat aaagaactgt tcaatcttgg aatggtaatg gttggcaggt ggtgctgttt
      901 ttttaccttg agtatttttt cacgttaaaa tttaataaag acaaacacct gtttttttca
      961 tggaggagaa tttaagcttg aataaaatta ttgaaaagga aaatttatcc gagagcgtga
     1021 tccgaatggt tctggaggct ccagttgtgg cgcaaaagcg gaaggccgga cagtttatcg
     1081 ttcttaagat tcatgaaaag ggggaacgca ttcctctgac cattgtcgat tctgatgcgc
     1141 agaagggagc gatcaccatt atttttcaaa ttgtgggcaa gacaacggcg ttgcttgcgg
     1201 ccatggctcc gggagatggg attcaggatg tacagggacc cttgggcaat ccgacggaga
     1261 ttgaaaattt tggccgtgtc gtttgtgtcg gcgggggcgt cggtgtcggc gttgtttttc
     1321 cgctgattgt cgccttgaaa aaagcgggca atgacgtggt ttccatcatc gggtcacgca
     1381 caaagaacct gctgattctg gaagaagaga tcaaagcgaa ttccgatcgt ctggtggtca
     1441 ccaccgatga cggaagttac ggcgttcacg gttttgtcag caatgttctt caggagatga
     1501 ttgacaaggg cgaaaagatc gatcgtgtgt ttgccattgg ccccgtgccc atgatgcgtg
     1561 tcatttgcga tgtgacaaag ccgcacggca ttaaaaccat cgtcagtctt aattcgatca
     1621 tggtcgatgc tacgggaatg tgcggagcct gccgtgttga tgtgggtgga aaaaccaggt
     1681 ttacctgtgt ggacggtccc gaatttgatg gtcaccaggt cgattttaaa ttactggccg
     1741 accgtttgag gatgtattgc gatcaggaac gggaagccta tgaaaaacac aagtgtgggt
     1801 gtgatggaaa ctaaagagac gaagccaaaa aaagaaaaag tgccccggca ggcaatgccg
     1861 gaacaggatc ccaaagagag gattcacaac tttgcagaag tgcctttcgg ctactccgaa
     1921 gaaacggcga ttattgaggc gcagcgctgc atccagtgta agaaacccgg atgcgtcagc
     1981 ggatgtcccg tagatgtcaa gattcccgaa tttatcaagg ccgtcgcgga aggtcgtttc
     2041 attgatgccg cattgaaatt aaaagaaacc aacggtcttc ccgccgtctg cggccgtgtc
     2101 tgcccgcagg aagatcagtg tgaaaaggaa tgtattctcg gcaagaaagg cgagcccgtg
     2161 gccatcggac ggctggagcg tttcgcggcg gactatgagc gcaacagcgg caaaatttcc
     2221 attcctccgg ccgtgcaatc aaacggcaaa aaaattgccg ttgccggcgc cggtccagcg
     2281 ggattaacca ttgcgggcga tctggttaaa ctgggttacg atgtcaccat ctttgaagct
     2341 ctgcataagg ccggcggcgt gctggtttac ggcattccgg agtttcgttt gccgaaggcc
     2401 attgtggaag cggaagtgga ctatctcaaa aaaatgggcg ttaagataga aacgaatttt
     2461 gtcatcggcc gcacgaaaac aatcgatgaa ctttttgccg atggatttga tgccgttttc
     2521 atcggggtgg gcgcaggcgc tccggtcttt atgaacatcc ccggcgagaa tctgagcggc
     2581 atctattcgg cgaatgaata tctcacgcgc tccaatctga tgaaggccta tttgtttccc
     2641 gaatacgaca ctccgattgt tcgcggcaag aaggtcgccg tgatcggcgg aggcaatgtc
     2701 gcgatggatt ccgtgcgtac ggccttgaga ctcggcgcgg aaagggcgat gattatttac
     2761 cgccgtaccg aagtggaaat gcctgcgcgt aaagaagaag ttcaccacgc gcgagaagag
     2821 ggcgttgaat tcctcctgct gcacaatccg gtggaatata tcggcaacga agccggctgg
     2881 gtcactggga tgaagtgcat aaaaatggaa cttggggagc cggatgcgtc cggaagacga
     2941 agccccgttc ccattcaagg atccgaattc gtgattgatg tggataccgt tgtcgttgcc
     3001 gtcggcacca tggccaatcc gattgtcccc gcaaccacca ccggtttgga aacgaataag
     3061 aaaggatata tactaaccaa ggatgaatcg gggctgacca gccgcgaagg catttatgcc
     3121 ggaggcgata ttgtcaccgg ttccgcaacg gttattctcg cgatgggcgc agggcgcaag
     3181 gcggccaacg cgattcacga gtatctttcc aataaatagt tcaatccggc agcccgggcc
     3241 tgatcccggg ctgtcaatgt atgatcagat gcacggaccg gcatagaaaa gccaagccga
     3301 atcccgccag acacatgccg gcgatcaacg aaacaaatcc gataaatttt tcattgacga
     3361 tttttctccc ccagtgcagc gcggcggaga gaaaaacgat ccagatgaag cagccgagca
     3421 atatagtgag acacgaaaga tatgcggaaa aatttgtttg tatactgtct tgagatacca
     3481 gcagagcgcc gaaaacaccc gtccaccaga cgatggtgat cggacaggaa agcgcaatta
     3541 cgtaaccggc gacgtaggga tttttaaaaa aacgccgatt ctgtttttca tcatagtccg
     3601 ttttgtccag gaaatctttt acgctgctta cgccgagata aattaaaatc agactgccgg
     3661 ctagggcaaa aaatattttc atcaaaggca tcttcaaaaa agaaacaaga ccgaaaaaga
     3721 tgattgtcag atacgttgcg tcggcggtca tggcgcccaa tccggtcgca aaagcctgtt
     3781 tgaatcccga attcaaaccg cgtttgacga tttctacatt caccgggccg aacggagcgg
     3841 ccagcgaaag ccccagaaga atattttgca gtaatgaaaa tttcatgctg tttttgatac
     3901 cacaaaagca gccgccctgt cactttctcg ttatgcaaat ttccgttctt cttttgtcgg
     3961 catgtttcac atttttatgt tgataaatta ttttttcata tcttgctgca aaaacatcag
     4021 ttttcaaggg gaatttttat gcggctggcc gccgttaaaa ataacaaata ttatagtcat
     4081 atcttattgt tacgataatg cttgaccgtc tttgacggtc aggttaaaat taacaagttg
     4141 ttagtgcaca atgaattagg agggcagaaa aatggctaaa tttgaatata acgaggaagc
     4201 ggtcaagaat ctgaaattac gtgacacatc cgttttggac cgcttgatag atttcgaatc
     4261 ggcgcgcgaa gcgcattcga aaaaagaaca cgccaagaaa gacaaacgaa tgaccatagg
     4321 tgaagctgtt gcgacatttg tgcaggatgg cgacatcatc accgatggcg gcttttctta
     4381 cgtcaggacg ccgcatcagg cctttcacga ggttatcagg cagggtaaaa agaatttgca
     4441 gatgatcgga tcacccaata cgaatcagag tttttttatc ttttacggcc tggtgaatta
     4501 ttcgcataat tcatattccg gagcggaaat gcgcgggatt gacaggctct atgatcgtgc
     4561 gttgaagaac gacagggtta ccgttctttc cgagt
//
LOCUS       DAOOLB010000129         4591 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_22637_length_4591_cov_55.489198, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000129 DAOOLB010000000
VERSION     DAOOLB010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22637_length_4591_cov_55.489198"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /locus_tag="PLF77_10490"
     CDS             complement(<1..1775)
                     /locus_tag="PLF77_10490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPC09325.1"
                     /translation="MWKDKVVSPSEVLSQIEPGMTIFLGTGMAEPRTLVKNLMASEEP
                     NLQDLELIQLVSIGDTIPIDERYSRKFRLRTFFSGWIASEAVSAGRIDLIPSRFSKIP
                     QLFKSGAIHIDATFIQISPPDENGYACLVGVDVERQAMETAHLVVGEINAKAPRIMGD
                     TLVHMDEFNFFVESAEPPLYIPRWPVEDVFLKIAANIAAVVEDASCVSFGIGPLYEAL
                     AVQLTTKKNLGVHSPFFTDSLMDLVQSGAVTNRYKGVFRGKCAASYLLGSEKLMRWLD
                     RNPLVEFQPEDVITDPKIIGTNDKTISILPARKIDLTGNVALHTGKGNVTAGPGNVQE
                     LFIGASLSKNGRTIFGLPSRNRKGLPNIVLSVDNLPFQFTNRESMDLVATEYGVAYLM
                     GKTMRERAQELIDIAHPDDREELVRQAKEAKLLYADQIYFPESGHLYPARFICSHEFK
                     GGLKVLFRPIKPSDEEKMRDLFYRFSDQAVYSRYFTSIKTMPHKKMQEYVNVNYRWVM
                     SIVGVVEAAGTEKIIAEARYARTKQDAFADVAFVVDEDYQNMGIASYLFELLIRAARE
                     EGIKGFTADVLASNKSMLKVFEKAP"
     gene            1996..2826
                     /locus_tag="PLF77_10495"
     CDS             1996..2826
                     /locus_tag="PLF77_10495"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HPC09326.1"
                     /translation="MALSGFRRQSIFALFFLLLVLPDTASAVNFYDGARAKQGTYFLT
                     YTSVYAADKTTNGKGNHASKDMGLLNAQELLRLCYYSPDFVATALVPFGLMKIDSLDQ
                     ESSGIGDINLGAGYFLPIRNVDILPMIFVEYPTGQYSSAKSANIGSNQLDIKPVVFLH
                     KSMGDFSIDAAVKYVFRFETERTDVLPGDEFHLQCLLGYHLTEKFKLGPSVNWLQSKE
                     KKQNGTAVDDSARAVLSVGADIYYRFSRFGITLTYLCDVYTENSAQGHFVQIKTVYRF
                     "
     gene            complement(2823..3989)
                     /locus_tag="PLF77_10500"
     CDS             complement(2823..3989)
                     /locus_tag="PLF77_10500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09327.1"
                     /translation="MSFNERTYVATDSLCPPVANQFVFDGEGVLDPYLWRTAVAVASE
                     ANPGARLILKGHLAWSRWIDSGNPPRVREIDGSRWDGHGPEGAPVLLEPLPFRESPIC
                     EVILVHGPVPRVVFRTHHGVMDGRGTLCWAEDVFRALRKENVMGSTSTLTEVQMAESF
                     QKQFRRAFPVEHIAPTGLPEGDARGVIWKRRSIKGKVPLLLPRCARFAAEEAWRHAEG
                     IVRFAVAVDMRPRRPELRSTGNLTLDLYVEINKNTTPELIADDMAYQLRHGFEGRIKK
                     YDQLVKYIPLRLVRFGAQKMIRDRHRKGRYGLSGIFSNLGRVDLNFFCGGGFSARSFW
                     AVPPATEYFPFFLVLSGYEGGSELVLSVPKNLGSAGRLDDTLECIARKLEQVTR"
     gene            complement(4181..4591)
                     /locus_tag="PLF77_10505"
     CDS             complement(4181..4591)
                     /locus_tag="PLF77_10505"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HPC09328.1"
                     /translation="MRDKTLKEGEVIVIRYEGPRGRPGMPETLAVTMGLDLAGFTNVA
                     LVTDGRFSGATAGPCIGHVSPEAASGGPIVVIEDGDMINIDIPARRLDVDLKPQEIAR
                     RLAGWKPLHKQITPGYMRRYVRMVGSAAYGAVLE"
BASE COUNT         1118 a         1257 c         1127 g         1089 t
ORIGIN      
        1 aaaggcgctt tttcaaaaac tttcagcatg gatttattgg aagccagcac gtcggcggtg
       61 aatcccttga tgccctcttc gcgcgccgca cggatcagca gttcgaaaag atacgaggca
      121 atccccatat tctgataatc ctcatccaca acaaaagcga cgtcggcaaa ggcatcctgt
      181 ttcgtgcggg cgtatcgcgc ttcggcaatt attttttccg tgcccgccgc ttcgacaacg
      241 ccgacgatgg acatgaccca gcggtaattg acgttgacgt attcctgcat ctttttatga
      301 ggcattgttt taatggacgt gaagtagcgg gaataaacgg cctgatcgga aaaccggtag
      361 aataagtccc gcatcttttc ttcatcggac ggtttgatcg ggcggaaaag aaccttgaga
      421 cctcccttga attcatgcga acagatgaat ctggccgggt agagatgtcc ggactcgggg
      481 aaataaatct gatcggcgta aagcagtttc gcttccttcg cctgacggac cagttcctcc
      541 cgatcgtcgg gatgcgcgat atcaatcagt tcctgagcac gctcgcgcat cgtcttgccc
      601 atcaaatacg ccacaccgta ttccgtcgcc accaggtcca tcgactcacg gttggtaaac
      661 tggaacggca gattatcgac ggacagaacg atattgggca gacctttccg gttgcggctg
      721 ggcaatccga aaatggtacg gccgtttttg gaaagcgacg ctccgataaa aagttcctgg
      781 acatttcccg gaccggcggt gacattcccc ttgccggtat gcagcgcgac attgcctgtc
      841 aggtcaattt ttctggccgg cagaatggat atggttttgt cattcgtgcc gattattttc
      901 ggatcggtaa tcacatcctc cggctgaaat tcaaccagcg gattgcggtc cagccagcgc
      961 atcagttttt cactgcccag cagataggac gcggcacact tgccgcggaa cacgcctttg
     1021 tagcgattgg tcacggcgcc gctctgaaca aggtccatca gagaatccgt aaaaaaagga
     1081 gaatggacgc cgagattttt tttcgttgtg agctgaaccg ccagggcttc gtaaagaggg
     1141 ccaatcccga atgatacgca actggcgtct tcaacaaccg cggcaatatt ggcggcgatt
     1201 ttcagaaaaa catcttccac cggccagcgg ggaatataca acggcggttc tgcggattcg
     1261 acaaaaaaat taaactcatc catatgaacc agtgtgtcgc ccatgatgcg gggcgccttg
     1321 gcattgattt cgccgacaac cagatgcgcg gtttccatcg cctgccgctc cacatccacg
     1381 ccgaccaggc aggcataccc gttttcatca ggcggagata tctggatgaa cgtcgcatca
     1441 atgtgaatgg cgcccgactt aaacagctgg gggattttgg aaaaccggct ggggataaga
     1501 tcgattcttc ccgcgctgac ggcttcggac gcaatccagc cggagaaaaa agtcctcaga
     1561 cgaaatttgc gggaatatcg ttcatcaatc ggtatggtat cgccgatact gaccagctga
     1621 atcaactcca gatcctggag gttcggctct tccgacgcca taagattttt aaccagcgtg
     1681 cgcggttccg ccatgcccgt ccccagaaaa atggtcatgc ccggctcgat ttgagacagt
     1741 acttcactgg gagaaacaac cttatccttc cacatacatt attccctttt tcagttgtag
     1801 ggttaaaatt taatttacgc cgttgcgaga ccgaacgtct gaattatagg gaggcggcct
     1861 gtgcctgtca agacaattaa acctttttaa acagatatgg aaggatggcc gacaggtgaa
     1921 atatttatgc tttgcccccg gcgaaatgtt ctgtataagc atattctcaa aaaaaacggt
     1981 ttcgaggatt caattttggc tttatcgggt ttccggcgac aatccatttt cgcgttgttt
     2041 ttcttgttac ttgtgctgcc tgatacggcg tctgccgtaa atttttacga cggtgcaagg
     2101 gcaaaacagg gaacttattt tctcacctac acttccgttt atgccgctga taaaacaaca
     2161 aacggcaaag gcaatcacgc gagcaaagac atggggctgc tgaacgccca ggaattgttg
     2221 cgtctttgct attattcgcc tgattttgtg gccacggctc tggttccgtt cggtttaatg
     2281 aaaatcgatt ctctggatca ggaatcttca gggattggcg atatcaatct cggcgccggt
     2341 tattttctgc cgattcgaaa cgttgatatt ctcccgatga tatttgtgga atatcccaca
     2401 ggccaataca gctctgcaaa atcagccaac atcggctcga atcagctcga cataaaaccc
     2461 gttgtttttc ttcacaaatc aatgggcgac ttcagcatag atgccgctgt gaaatacgtc
     2521 ttccgttttg aaactgaaag aaccgatgtt ctgccgggcg acgagtttca tctgcagtgc
     2581 cttctgggtt accatctgac ggaaaagttc aaactgggcc cttcggtcaa ctggttgcaa
     2641 agcaaagaaa aaaaacaaaa cgggacggca gtcgacgaca gcgccagagc agtcctttcc
     2701 gttggggccg atatttatta tcgcttttcc cggttcggta tcaccttgac ttatctttgc
     2761 gatgtttata ctgaaaattc agcgcaggga cattttgtgc aaataaaaac ggtttatcgc
     2821 ttttagcggg ttacttgttc caatttccgg gcgatgcatt ccagagtgtc atcaagtctt
     2881 cccgcactgc ccaggttttt gggaaccgaa agcaccagtt ccgaaccgcc ttcatatccc
     2941 gacagtacca gaaaaaacgg aaaatactcc gtcgccggcg ggacagccca gaacgaccgg
     3001 gcggaaaatc cgcctccgca gaaaaaattc agatcgaccc ggcccagatt ggaaaaaatg
     3061 cccgaaagcc cgtagcgtcc cttgcgatgc cggtcacgaa tcatcttttg cgccccgaac
     3121 cgcaccagtc gcagcggaat atattttacc agttgatcat atttttttat ccggccttcg
     3181 aaaccgtgcc ggagttgata cgccatgtcg tcggcaatca gttccggcgt cgtatttttg
     3241 ttgatctcga cataaagatc caacgtcaga ttgccggtgc tgcgcaactc cggccgacgg
     3301 ggccgcatat ccaccgccac ggcaaaccgc acgatgcctt ccgcatgccg ccaggcttct
     3361 tccgcagcaa accgggcgca tcgcggcaaa agcagcggca ccttgccttt gatgctgcga
     3421 cgtttccaga taacaccccg ggcgtcgcct tcgggaagtc ccgtaggcgc aatgtgttcg
     3481 acgggaaaag cgcggcggaa ctgcttttga aaactttcag ccatctgaac ttccgtcagg
     3541 gtcgacgtcg atcccatgac gttttctttc cgcagggccc gaaaaacgtc ctcggcccag
     3601 cacagcgttc ctctgccgtc catgacaccg tgatgcgtgc gaaaaacaac ccggggaacc
     3661 ggaccatgta caagaatcac ctcgcagatc gggctctcac gaaacggcag cggttccagt
     3721 aaaacaggcg ctccttccgg cccatgaccg tcccatcggc ttccgtctat ttcccgaaca
     3781 cgcggcggat tgccggaatc gatccagcgg ctccaggcca gatgaccttt gagaatgagc
     3841 ctcgctccgg gattggcttc agaggcaacc gccacagcgg tgcgccagag atacggatca
     3901 agcacacctt ccccgtcaaa aacaaactgg ttggccaccg gagggcagag cgaatccgtc
     3961 gccacatacg tacgttcatt aaaactcatt tttcgcatgt accccttatc cacagcagca
     4021 cctttcaatg ataatttcat taattttatc acatcttcat acgtgcttca gttactttat
     4081 gatggcttct gccctcaaga aaaaaatcag gtttcaaaca aatgtctgaa acctgacggg
     4141 attgaaaaaa tgaatcccac cggaatactt tcaagcggat tcactcaaga acagcgccat
     4201 atgccgccga gccgaccatc cggacataac ggcgcatgta gccgggggta atctgcttat
     4261 gcagaggctt ccaaccggcc agcctccggg cgatttcctg cggtttcaaa tccacgtcaa
     4321 gacggcgcgc ggggatgtcg atgttgatca tgtcgccatc ttcaataacg acaatcggtc
     4381 cgccgctcgc cgcctccgga gaaacatgtc cgatgcaggg tcccgccgtg gctcctgaaa
     4441 agcggccgtc cgttaccagc gccacattcg tgaaaccggc caggtcaagt cccatggtca
     4501 ccgcaagcgt ctcaggcatg ccgggtcggc ctctgggacc ttcgtaacgg atgacaatga
     4561 cttctccttc cttgagcgtt ttatcacgga t
//
LOCUS       DAOOLB010000130         4559 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_22858_length_4559_cov_43.702931, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000130 DAOOLB010000000
VERSION     DAOOLB010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4559
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22858_length_4559_cov_43.702931"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="PLF77_10510"
     CDS             complement(<1..275)
                     /locus_tag="PLF77_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09329.1"
                     /translation="MFVWGCAGGVKNEKKDAFFEKWTTLAENSQGHSPSAHPKKIDTS
                     ALIAKKDPASPMVGASDEAKKLPTSPIHLTMRQADLKAVLRAMAKSV"
     gene            complement(361..861)
                     /locus_tag="PLF77_10515"
     CDS             complement(361..861)
                     /locus_tag="PLF77_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09330.1"
                     /translation="MAKLKKREIVILAIAALFVLYAVYVYLIADRQKGKKVKPDQETV
                     KVEAVVSGITGELNKSKISDFDHYVIQRTQTDWDKNPFLKRELYRAWLAKDSKGKDGV
                     AAVPIIYSGYIETGKNRLAVLNGIEYRVGEALQEEGFVLKKIMPSKVVIFDKRIGSNL
                     EIPLQE"
     gene            complement(878..1417)
                     /locus_tag="PLF77_10520"
     CDS             complement(878..1417)
                     /locus_tag="PLF77_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09331.1"
                     /translation="MMKNFNLNAQQKKSILYAVICSGIVLIIVLVGILPYSFKVSNQQ
                     NENEKLTSQIKEQRELGPVYKTLTGNVEQTDVLILPHAQKTALPRQDITIIQKDFQAA
                     AQKSGLKIISYAPEINTSASPSASFLHNLELKGELAGFRKLLIALGALPYVERIEEIS
                     IQQNAGAMDFKMKIWTAVQ"
     gene            complement(1417..>2154)
                     /locus_tag="PLF77_10525"
     CDS             complement(1417..>2154)
                     /locus_tag="PLF77_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09332.1"
                     /translation="DYMGNQLDTRIEIFDPFKNRKSVISEQSLSSSERILISPALGFA
                     LSDNSRTPNAVFTYEEKRQDIKSRRINKVIFMAFLAALMVCIGTLFYQGSKFSTLKKN
                     NLALEKELALFSPMVSQETILQEVNRIKLQRDAVRQYAQKYLGVAVIGEISDTTPENV
                     RLINLRLRQAAGSPAAAGQKTEHNGLVLEGIIFNKKDMLESDLTQYILKLENSPLFNT
                     VSVQNKNIVNFDKKDVIHFVLSAKLGS"
     assembly_gap    2155..2254
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2255..2943)
                     /locus_tag="PLF77_10530"
     CDS             complement(<2255..2943)
                     /locus_tag="PLF77_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09333.1"
                     /translation="MSNTSKDINSTEKLLNVIRGKDEASFGGLGKQKISLTPDKKTGI
                     FQSGFLEKFLSKKKYTVGIDIGREFICLVKTAQDSNHQPILVDKKIITIDPRFVIGSP
                     EFNQLVKTSVLNFCGNVADCDIWTKISTSQVGVYFFTIPRVPKKQIQKVIFWTAKKEG
                     FYDEEKQIFDFEIHGELVEQGTPKYSVMFYTAQKAEVARIKSFADNIGINLKGITIVP
                     FAMQNIFRSKW"
     gene            3175..>4559
                     /locus_tag="PLF77_10535"
     CDS             3175..>4559
                     /locus_tag="PLF77_10535"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPC09334.1"
                     /translation="MNKKKKIIISCLILLLIGALFYFMNIYSTVAADIKLRGSSSPPL
                     TEKKQSAKPASSDHSSYKGQIPDSIVAFSSGYAVVVDKEHQKLYVFYKSGKYSKVFET
                     VCSTGKNPGSKRIAGDAKTPNGIFFATRILENPGPSETYGSLAFTLDYPTLSDKRAGK
                     DGNNIWIHGTTKPLLPQQSNGCVVLSDEDMKKLANFIHLNRTPVIISESINWVDQDYL
                     SDSRDEFEKILWSWNKAFVEKNLKEIDSLYAEGAQIRGKKREDLHYKIKNLDFIKKHF
                     VLQPRDIAILQEGDNAVIIFDQIFDVKDNNTFQGFYNKLVLEKINENWYVVDESSISE
                     PADRRLATAKDTQKDAVSPEKIAEKNIRSLVRKWRASWESGSMKTYRACYASDFQSRN
                     MGLDAWIAHKIKVRQKSSNIKISVNNLQISISGNTARATFVQQYSSSILRTKGSKTLE
                     LKKSADGWKIH"
BASE COUNT         1258 a         1001 c          889 g         1311 t
ORIGIN      
        1 ttcaccgatt tggccattgc ccgcaacacc gccttgagat ccgcctggcg catggtcaga
       61 tgaatgggcg atgtgggaag tttctttgcc tcgtcactgg cgcccaccat tggacttgca
      121 ggatcttttt ttgcaatgag ggccgacgtg tcaatctttt tggggtgcgc cgaaggggaa
      181 tgaccttgag aattttcagc aagcgtcgtc cacttctcga aaaaagcgtc ctttttttca
      241 tttttgactc cgccggcaca gccccataca aacatgagtg ctgtgcaaca aaaaaccaaa
      301 gtcatgattt ttttattcat tatagagttt ctcaaggcga taacctccaa ttgacaaatt
      361 ttattcctgc agcgggattt ccagattact tccgattcgt ttatcaaata ttacaacttt
      421 ggaaggcatg atttttttca agacaaaccc ttcctcctgc aatgcttcac caactctata
      481 ttcgatgccg ttaagcacgg cgagtctgtt tttccccgtc tctatataac ccgaataaat
      541 gatcggaacg gcggcaaccc cgtctttgcc tttgctgtct ttggccagcc aggctctgta
      601 taattctctt ttcaggaaag gattcttatc ccaatccgtc tgcgttctct ggattacata
      661 atgatcaaaa tcggaaatct tacttttatt caactcaccg gttatcccgc tgacgaccgc
      721 ttcaaccttc accgtttcct gatcaggctt cacttttttg cctttctggc gatcagcaat
      781 cagataaaca tagacggcat acaagacaaa cagcgcggct attgctaaaa tgacaatttc
      841 tcttttcttc aatttggcca taaatccctt tacctgccta ttgaacggct gtccaaatct
      901 tcattttaaa atccatggcg ccggcgtttt gctgaatgct gatttcttcg attctttcca
      961 cataaggaag ggcgcccagc gcgatcagca atttgcggaa tccagccagt tcccctttca
     1021 gttccaggtt atgtaaaaac gatgcggatg gactggccga tgtattaatt tccggagcat
     1081 acgaaatgat ttttaatccg gatttctgcg ctgccgcctg aaaatccttc tgaatgattg
     1141 taatgtcctg gcgaggcagc gccgttttct gcgcatgcgg cagaataaga acatcggtct
     1201 gttccacatt gccggtcaat gtcttataga caggcccgag ctctctttgc tccttgattt
     1261 gtgacgttaa tttttcattt tcattctgct ggttggatac tttgaacgag taaggcagta
     1321 tgcccaccaa aacaataatt aacacaattc cgctgcagat cacggcgtag agaatacttt
     1381 tcttctgttg cgcattcaga ttaaaatttt tcatcatcac gaacccagtt tggcgcttaa
     1441 tacaaaatga atgacgtctt tcttatcaaa gttaacaata tttttatttt gcaccgacac
     1501 cgtattgaaa agaggagaat tttccagttt taaaatgtat tgtgtcaaat cggattccag
     1561 catatccttt ttattaaaaa tgatgccttc gagaacaaga ccgttatgtt ccgtcttttg
     1621 accagccgct gcgggagaac cggcggcttg ccgcaggcgc aaattaatga gacgcacgtt
     1681 ttccggggtc gtatccgaaa tctctccgat caccgcgacc cccagatatt tttgcgcata
     1741 ctgccggacc gcgtctctct gtaatttgat cctgttcact tcctgaagaa tcgtttcctg
     1801 cgacaccatc gggctgaaaa gcgccagttc tttttccagg gccagattat tttttttcag
     1861 cgtactgaat ttggatccct gataaaataa cgtaccgata caaaccatca gcgccgccag
     1921 gaaggccatg aaaataacct tattgatccg ccgtgattta atgtcctgac gtttttcttc
     1981 ataggtaaat acggcattgg gcgtgcggct gttatccgac agggcaaacc ccagagcggg
     2041 agaaatcagt attctttcgg aagagcttaa cgattgttct gaaataaccg attttctgtt
     2101 cttgaaagga tcaaatattt caattcgggt atccaattga ttgcccatgt aatcnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatccat ttcgaccgga aaatattctg
     2281 catggcgaaa ggaacgatgg taattccttt aagattaatg ccgatattat cggcaaacga
     2341 ttttattctc gcaacttccg ctttttgagc ggtatagaac atgaccgaat atttaggcgt
     2401 cccctgctct accagttcgc catggatttc aaaatcaaaa atctgttttt cttcatcgta
     2461 aaacccctct tttttggccg tccagaaaat gactttttga atttgctttt tgggaacacg
     2521 gggaattgtg aagaaataaa cgccgacctg acttgtggat atcttggtcc agatgtcgca
     2581 atcggcgaca ttaccgcaaa aatttaagac cgacgttttg accaactgat tgaattccgg
     2641 agaacctatg acgaaccggg gatctattgt gatgattttt ttatcgacta aaatcggctg
     2701 atgattgctg tcttgagctg tttttaccag gcagataaat tcacgcccga tatcgatacc
     2761 gaccgtatac ttctttttgg aaaggaattt ttcaagaaat cctgattgga aaataccggt
     2821 ttttttatcc ggcgttaaag aaattttctg ttttccgagc ccgccgaaag acgcctcgtc
     2881 tttcccgcgt atcacattga gaagtttttc cgtagagttt atgtctttgc tggtattaga
     2941 caaaataatc tcctggtcgt tgacgcatgc accaatctaa taaatcattt ttttaaattt
     3001 ataatgtaat tccaaattcc tgtaaatcat tttattagat tctttgttct cacaaaaaaa
     3061 agaaagcctc acagaagagc aatttttatt ttgtattaat ttcttgaatg attatcagtt
     3121 ttttgctaaa agaacaatac tttttttaaa aaaactctga atttacctgt tcttatgaat
     3181 aagaagaaaa aaataataat ctcttgtctc atccttctgc tgatcggcgc tctgttctac
     3241 ttcatgaata tttattccac cgttgctgca gatattaaat taagaggctc ttcatcgccg
     3301 cccttgacgg aaaaaaagca aagcgcgaaa ccggcgtcat cagaccattc ttcctacaaa
     3361 ggtcagatac cggattcaat tgttgccttt tcgtcaggat acgcggtcgt tgtcgataaa
     3421 gaacatcaaa aattatatgt cttttataaa tcaggcaaat actccaaggt cttcgaaacg
     3481 gtttgttcca ccggtaaaaa ccccggttcc aaaaggattg cgggagatgc aaaaaccccc
     3541 aatggaattt tcttcgccac cagaatcctg gaaaacccgg gaccttcgga aacgtacggt
     3601 tcactggcct ttacgctgga ttatccgact ctgtccgata aacgagcagg caaagacggc
     3661 aataatatct ggattcacgg aaccacaaag ccactgcttc cgcaacaatc caacggttgt
     3721 gtcgtgctga gtgatgaaga tatgaagaaa ctggccaact tcattcattt gaacagaaca
     3781 cccgtcatta tctccgaatc catcaattgg gttgatcagg attatctttc cgactccaga
     3841 gatgaattcg agaagatatt atggtcgtgg aacaaagcat ttgttgaaaa aaatttaaag
     3901 gaaattgatt ctttgtatgc tgaaggcgcc cagatcaggg gaaagaagcg agaagacctt
     3961 cactacaaaa ttaaaaattt ggatttcatt aaaaaacatt ttgttttaca gcctcgcgac
     4021 atcgccatcc tgcaggaagg cgataatgcc gtcattattt ttgatcagat ttttgacgtg
     4081 aaggacaaca atacttttca gggattctat aataaactgg ttttagaaaa aataaacgaa
     4141 aactggtatg ttgttgatga gtcctcgata tccgagcctg cggacaggcg cctggcaaca
     4201 gccaaagaca cgcaaaagga tgccgtctcc cccgaaaaaa ttgccgaaaa aaacatccgg
     4261 agcctggtca gaaaatggcg ggcaagctgg gaatcgggca gcatgaaaac atatcgggcc
     4321 tgttatgcgt ctgattttca atcccgtaac atgggactcg atgcctggat agcgcataag
     4381 attaaagtac gccaaaaaag cagcaacatt aaaatcagcg tcaataattt acaaatttcc
     4441 ataagcggca atacagccag ggcaacattt gtgcaacagt acagttcttc cattttaaga
     4501 accaaaggtt ccaaaactct ggaattgaaa aaaagtgccg acggatggaa aatacatag
//
LOCUS       DAOOLB010000131         4537 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_23012_length_4537_cov_71.742972, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000131 DAOOLB010000000
VERSION     DAOOLB010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23012_length_4537_cov_71.742972"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="PLF77_10540"
     CDS             complement(<1..800)
                     /locus_tag="PLF77_10540"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HPC09335.1"
                     /translation="MSKRMKVIIAFVLIMQMVIPYPVYAATVGKFTSVVGNVILTRDG
                     KDYRPVVNSAVNEKDLIVTGDGASAVMVFTDDSTITLSPKTRMQVREFGMEGKARKGI
                     FSLSIGKLVANVTKFIGGNNKFEVHSPTAVCGVRGTLFGLEVAMVGTVMTTTATCTTG
                     ALSVSALSATGAIVATSGIVAGQTAVITSTGITVGAAGAAGAAGAGAAGSAGTTGAAG
                     AGAAGTTGAAAGTAGAGAAGAAGGAAAAAGIGAGTIATAAAVAAAAVAA"
     gene            1004..2314
                     /gene="eno"
                     /locus_tag="PLF77_10545"
     CDS             1004..2314
                     /gene="eno"
                     /locus_tag="PLF77_10545"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HPC09336.1"
                     /translation="MPEIINIHAREILDSRGNPTVEAEVTTIAGITARAAVPSGASTG
                     EHEMLELRDDNKKRYNGKGVEKAVRNVLHKIAPEIIGMDCRNQRDIDFAMIELDGTAN
                     KGKLGANAILSVSMACAKAAAEISGLPLYRYLGGVNANVLPIPQSNILNGGQHADNNV
                     DIQEFMILPVGAPNFKEGIRMNAEVFHALKAVLKGKGLNTSVGDEGGFAPNLKSNEEA
                     LTCIMTAIEKAGYKAGKDIFIALDCAASSFYEKGKYVLAAETKPRKSSEELVKFYADL
                     VAKYPILSIEDGLAEDDWTGWKLMTEELGGKIQIVGDDLFVTNVRRLNKGIELGIANS
                     ILIKLNQIGTLTETLETMSRARECGYTCVVSHRSGETEDTFMADIAVATNCGQIKSGS
                     LSRTDRLAKYNQLMRIEEELGDAACYRGRDAFYNLKKTAGAKKK"
     gene            complement(2380..>4537)
                     /locus_tag="PLF77_10550"
     CDS             complement(2380..>4537)
                     /locus_tag="PLF77_10550"
                     /inference="COORDINATES: protein motif:HMM:NF039580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HPC09337.1"
                     /translation="DAIASLMKLQAGLSFPVYVVPVLVAYGRRREKEDESLFNILFGQ
                     TEHTGALRRLITFMRYSKQAFIIPAKPVHLAEYLSANSHLTHNELVMDLRGELIDRIE
                     EEKTSVVGPELKSREAVIGMVMHDPKLNRFLDDYAQKSKKDRTGLARDARRYLHEIAS
                     DYRETFVELWDKVLTWLWNTVYDGVSIDSEGLANVRNLSKKMPFVIISCHRSHIDYLL
                     LSYVFYKHNIQMPFIAAGNNLSFFPMGYIFRRSGAFFLRRSFRGNEVYGEVFSKYMAT
                     LLKEGLPLEFFIEGGRSRTGKMVMPKYGLLSMIIQAYQDKYCENLAAVPVYIGYDRVI
                     EEKTYLKELSGAPKVQENTVEIIKTTKILRKRYGRVYVNIGTPIVLKDYLAAQEKSMD
                     EMTVEERQSLYRKIGYEVVLEINKVSVVTPFALVASGLLSHDRRGISHEELQGILNEF
                     YEYLSTRQVKFADTFAHRDRAITDALNIFDETDIISKIEAEEEEEEMQEVVYSLTDAR
                     RLNLEYYKNNILHFFIPLCFVATSMVKNPEDSMSLAKIMGDYRFLKGLLWNEFIFDEN
                     REDAEDVTEALTYLHSRAMILTSRRDGEAWVEIKSRGGIKLKPFAGLIHNYLESYWIV
                     IRSLVYLKKKPLPQKEWLAKIRSLGDRMFRKGEVLRAEALSQANYQNAILFLESAEMI
                     VSQSREEKGGKREILYSLANNRAQLEVLRRRLFKFL"
BASE COUNT         1077 a         1297 c         1146 g         1017 t
ORIGIN      
        1 gccgcgaccg ctgcggctgc cactgcagct gcggtagcaa ttgtcccggc gccgataccg
       61 gctgccgccg ctgctccacc ggccgctcct gccgcaccgg cccctgccgt gcctgccgcc
      121 gctccggtcg tgccggccgc gcctgctcct gcagctcccg ttgtacctgc cgaaccagcc
      181 gcacctgctc ctgcagcacc cgctgcccca gccgcgccga cggtaattcc cgtggatgta
      241 ataacggccg tctgacctgc aacaatgccg gacgtcgcca cgatggcgcc ggtggccgac
      301 aaagcggaca cggacagggc gcccgtcgta caggtcgcag tcgtcgtcat gacggttccg
      361 accatggcca cttccagccc gaacaaggtt ccgcgaacac cgcagacggc tgtcggcgaa
      421 tgaacctcaa acttgttgtt gccgccgatg aatttggtta cattggcaac cagctttcca
      481 atggacagtg aaaagattcc ctttctggcc ttcccctcca tgccgaattc tctgacctgc
      541 atccgcgtct ttggggacag ggtaatggtg ctgtcgtcgg taaaaaccat gaccgcggaa
      601 gcgccatcac ccgtaacgat taaatccttt tcgttgacgg ctgaattgac gaccggccgg
      661 taatctttgc cgtcccttgt caggatgaca tttcccacca ccgaggtaaa cttgcctacc
      721 gtggcggcgt ataccggata tggaatgacc atctgcataa tcaatacaaa cgctataatg
      781 actttcattc gttttgacat atcggcctcc ctgaataatc ggttaagaaa aaccaaagaa
      841 aaaacggctt ttttttccta cttttccctt cgtttgtcaa atactttttt ccatgattca
      901 gatattcgac ttctttttct taagtcattt gtatcgcccc ggaaacccca tcttgaaatc
      961 agccggcatc tttgttataa agttaaaaaa aggaggtttc tatatgcctg aaatcattaa
     1021 tattcatgcg agagaaatcc tggattcccg cggaaatcca accgtcgaag ccgaagttac
     1081 aacgatcgcg ggcatcaccg ccagagctgc cgttccttcc ggcgcttcca ccggagagca
     1141 tgaaatgctg gaattgcgcg acgacaacaa gaaaagatac aacggaaaag gcgtggagaa
     1201 agccgtcagg aatgttttgc acaagattgc tccggaaatc atcggcatgg actgccgcaa
     1261 tcagcgcgac atcgatttcg ccatgatcga actcgacggc accgccaata agggaaaact
     1321 cggagccaac gccattttgt ccgtctcgat ggcctgcgcc aaagcagccg cggaaatctc
     1381 cggcctgccg ctgtaccgct acctgggcgg cgttaacgcc aatgtcctgc ccattccgca
     1441 gtccaatatc ctcaacggcg ggcagcatgc cgacaataac gtggacattc aggaattcat
     1501 gattctgccg gtcggcgccc cgaatttcaa ggaaggcatc cgcatgaacg ccgaagtgtt
     1561 ccacgctctg aaagccgtgc tgaaaggcaa aggcctgaac acgtcggtcg gcgacgaagg
     1621 cggattcgcc ccgaacctga aatcgaacga agaagctctg acgtgcatca tgacggccat
     1681 cgaaaaagcc ggctacaagg ccggcaagga cattttcatt gctctcgact gcgcggcgag
     1741 ctccttctat gaaaaaggaa aatacgttct ggcagcggaa accaaacccc ggaagtcttc
     1801 cgaagaattg gtgaagtttt acgccgatct ggtcgccaaa tacccgatcc tctccatcga
     1861 agatggcctg gcggaagacg actggacagg ctggaaattg atgacggaag aactgggcgg
     1921 caaaatccag atcgtcggcg acgacctctt tgtcacgaat gtccggcgcc tgaacaaagg
     1981 catcgaactg ggcatcgcca actccattct catcaaactc aatcagatcg gcacgctgac
     2041 ggaaaccctg gagaccatga gccgtgccag ggaatgcggt tacacctgcg tagtctcgca
     2101 ccgttccggt gaaacggaag acacgttcat ggcggacatc gccgtcgcca ccaattgcgg
     2161 ccagatcaaa agcggttcac tgtcccgcac ggaccgtctg gccaagtaca accagttgat
     2221 gcgcatcgaa gaagaactgg gcgacgcggc ctgctaccgc ggcagggacg ccttttacaa
     2281 cctgaaaaaa acggccggcg ccaaaaagaa ataaaagcgg cgataaaaca gcaaaaggcc
     2341 atattcccgc gatgggaata tggccttttt tgcgcaggtt cacaggaatt tgaacaggcg
     2401 tcgcctcagc acttccagtt gcgcccggtt gtttgccagg ctgtaaagga tttccctttt
     2461 gccgcctttt tcttcccggg actgggaaac gatcatttcc gcgctctcca aaaaaagaat
     2521 ggcattctga tagttggcct gcgacagggc ttcggcgcgc aggacttccc ccttgcggaa
     2581 catccggtct cccagtgagc ggatcttggc cagccattct ttttgcggaa gcggtttttt
     2641 cttgagatac accagactgc ggataacgat ccagtaggat tcaaggtagt tgtgaatcaa
     2701 gccggcaaag ggcttgagct tgatccctcc cctgcttttg atttccaccc aggcttcgcc
     2761 gtcgcgcctc gaggtcagaa tcatagctct cgagtgcaga taggtcagcg cctccgtcac
     2821 gtcttcggca tcctcccggt tttcgtcaaa aataaactca ttccacaaaa gtcccttcaa
     2881 aaaccggtaa tcccccataa ttttggccag cgacatggag tcttcaggat ttttgaccat
     2941 ggaagtcgcc acaaaacaca gaggaatgaa aaaatgaaga atgttgtttt tataatactc
     3001 cagattcagt cttctggcgt cggtgaggga atagaccact tcctgcattt cctcttcctc
     3061 ttcctcggct tctatcttgg aaatgatgtc cgtctcgtcg aaaatattca aggcgtccgt
     3121 aatcgcccgg tcccggtgag caaaggtatc tgcaaatttc acctgccggg tggacagata
     3181 ttcgtaaaat tcgttcaaaa tgccctgtaa ctcctcgtgg gaaatgccgc gccggtcgtg
     3241 actcaaaagg cccgaagcca ccagcgcgaa cggtgtgacc acggaaacct tattgatctc
     3301 cagcaccacc tcgtagccga ttttccggta aaggctctgg cgttcctcca ccgtcatctc
     3361 gtccatggac ttttcctgcg cggccagata gtccttcaaa acgatcggcg tgccgatgtt
     3421 gacataaacg cggccgtacc gtttgcgcag gattttcgtg gttttgatga tttcaacggt
     3481 gttttcctgg actttcggcg ctccggacag ctccttcaga taggtctttt cctcgatcac
     3541 gcggtcgtag ccgatgtaca cgggcaccgc agccagattc tcgcaatact tgtcctgata
     3601 ggcctggata atcatggaca gaagcccata tttgggcatc accatttttc cggtccggct
     3661 tcggccgcct tcgatgaaga actcgagcgg gagtccttcc ttgagcagtg tggccatgta
     3721 tttggaaaag acctctccat aaacttcgtt gccgcgaaaa ctcctccgca gaaaaaacgc
     3781 tccggaacgg cggaaaatat accccatcgg aaaaaaagaa agattgttcc ccgctgcaat
     3841 aaacggcatc tgaatgttat gtttgtaaaa tacgtacgac agcagcagat aatcaatgtg
     3901 actgcgatgg caggagatga tgacaaaagg cattttcttg gataaattgc ggacgttggc
     3961 cagcccttcg gagtcgatgg atacgccgtc atacaccgtg ttccacagcc aggtcagaac
     4021 cttgtcccac agctcaacaa acgtttcccg gtaatccgag gcaatctcat gcagatagcg
     4081 cctggcatcc cgggccagac cggtccggtc ttttttagat ttttgggcgt aatcatcgag
     4141 aaaccggttc agcttcgggt catgcatgac catgccgatc acggcttcgc gtgatttcag
     4201 ctccggcccg accacggatg tcttttcctc ttcaatccgg tcgatcagtt ctcctcgcaa
     4261 atccatgacg agttcgttgt gggtcaaatg actgttcgca gacagatatt ccgccagatg
     4321 aaccggcttg gcggggatga taaaggcctg tttggaatag cgcatgaacg tgatgaggcg
     4381 acggagcgct cccgtatgtt ccgtctggcc gaaaaggatg ttgaacaggc tttcgtcttc
     4441 cttttccctg cgccggccgt aggccaccag caccggcacg acatagacgg gaaaggaaag
     4501 gccggcctgc agcttcatca ggctggcgat ggcgtcc
//
LOCUS       DAOOLB010000132         4500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_23261_length_4500_cov_51.142632, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000132 DAOOLB010000000
VERSION     DAOOLB010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23261_length_4500_cov_51.142632"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="PLF77_10555"
     CDS             complement(<1..812)
                     /locus_tag="PLF77_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPC09338.1"
                     /translation="MERRVVITGMSAITPIGHGKDQIVQSLLNGTSGIKPLREEEFLS
                     SFIHSKVYGTVDYPVEYDFKRNHRKTMGPVAYYACQVAREVLEQSGLTEDFITSGRLG
                     VAFGSIHGSPTIQRNIYKIFFGAQDKIAVKEIGAVDYLKSMVHTTAVNITKMFGITGR
                     VISSGTACTTSSQSIGYGYESVKFGMQDAMLCGGADEYDTTTVAVFDNLLACSTAFNN
                     IPSKTPRPFDKDRDGLVVGEGAGAVMLEEYEHARKRGATILGEVIGFACNNNG"
     gene            complement(820..1089)
                     /locus_tag="PLF77_10560"
     CDS             complement(820..1089)
                     /locus_tag="PLF77_10560"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HPC09339.1"
                     /translation="MTRQEIEREVKNVFQREFEIDNPDMDADLRETYEFDSIDAIELL
                     LAIETFLNTEITQEEKKQAISIRTINQICDYIVEIAKKRNLFTPA"
     gene            complement(1161..2639)
                     /locus_tag="PLF77_10565"
     CDS             complement(1161..2639)
                     /locus_tag="PLF77_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HPC09340.1"
                     /translation="MRNSVDGKNIVIIGSGIGGLSTGIILSLMNFHVTVVEKNPLPGG
                     LMRSYRRGGIDCPVGIHYVGALGAEEPLGKMFAALGIPVDELFVPMAGEGIVDRYIFD
                     DLTFDLPDGIDTLENSLRDTFLQEKPALDVLMKNLRDISRQMTDPSFLFAQGDPFQNM
                     GYYQPMGELLDSLNVSPALRAVLSVPCNLIGVPLNDCPVIFHHMVLASYLFSSWRLKE
                     CGRKMTDVFIRRFEDLGGQLVLHDGVKKILFEKGKVSGVILESARTLFADAVVAAIHP
                     KVMLQLLDENVLRDSHRRRILGLKETESVIAVQVSVDAAKHPEMHHNIYRLHRDENGV
                     IQEGVFYQVQRTTSAGLHLLSIITKSLFGDWSRWENTVTGQRGSEYEEHKMNLAKGLL
                     KKAEDVFGDLNDARILDVYTPLTLRDYVSAPEGACYGIMRSSRQLLKAISLNNLPIPG
                     LCLAGQNAMAPGVMGCLFGAFNAVRQITGAERFAREINENMQ"
     gene            3096..>4500
                     /locus_tag="PLF77_10570"
     CDS             3096..>4500
                     /locus_tag="PLF77_10570"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC09341.1"
                     /translation="MEKISDYVLEKKIHKTKNAVTYRGYKEKDFQSVIIKLLNTLHST
                     PSEIAQFRQEYELIQRLDIGSVVKTIDIIHYKGGLALILEDFDGIPVKDYFRPNEKIN
                     IASFLALSANIAATLAALHARGVIHRNIKPQSLLINPETEELKITDFGISALLTHEHD
                     EVYNPDFIRDTLTYMSPEQTGRMNRSVDYRTDLYSLGITLYEIVTGLLPFKSQDPMEL
                     IHAHIAMMPRAPVKLDAGVPAMVSDIIMRLLAKNPEARYQNGFGLQADFQECLKQWEE
                     KKRIEPFELGRQDISNRFIVPQKLFGREKEISELISRFEEVANGDKRLSVMVVAGAPG
                     IGKSAMINEIHKPIVARRGYFISGKYEQFRRDKPYSAIIQAFQVLVKQILSESEQRIN
                     LWKKNLLSTLGDNGRVITDVIPEVEWIIGKQPDLPVLGPEETRNRFKFIFEKFMSVFP
                     QQEHPVALFLDDLQWADM"
BASE COUNT         1182 a         1097 c         1076 g         1145 t
ORIGIN      
        1 ccgttgttgt tgcaggcaaa accgatgact tctcccagaa tagtcgcgcc gcgctttctg
       61 gcatgttcat attcctccag catgaccgct ccggcgcctt cgcccacgac caggccgtcg
      121 cgatctttgt caaacggacg gggcgtcttc gacggtatgt tgttgaaggc tgtggaacag
      181 gccagcaggt tatcaaaaac agcaacggtg gtggtgtcgt attcgtcggc gccgccgcag
      241 agcatcgcat cctgcatgcc gaatttgacg gattcgtaac cgtacccgat cgactgcgaa
      301 ctggtcgtgc acgccgttcc cgacgatatc acccggccgg tgatgccgaa cattttggtg
      361 atattgacgg cggtggtatg caccatggat ttcagataat cgaccgcgcc gatttccttg
      421 accgcgattt tatcctgagc gccgaaaaag attttgtaaa tatttctctg aatggtcggc
      481 gaaccatgaa tggaaccgaa ggctacgccc agtcttccgg atgtgataaa atcctcggtc
      541 agaccggact gttccagaac ttccctggcg acctgacagg cgtaataggc caccggcccc
      601 atggtcttgc ggtgattccg tttaaaatcg tattccaccg gataatccac cgttccgtaa
      661 actttggaat gaataaagga gctgagaaat tcttcttcgc gcagcggttt gatgccggag
      721 gtgccgttta acaggctttg aacgatctga tctttcccgt gaccgatcgg cgtaatggct
      781 gacatccctg tgataacaac tctgcgttcc ataatgattt tatgccggcg tgaaaagatt
      841 tctttttttt gcgatttcga caatgtagtc gcaaatctga ttgatcgtgc ggatactgat
      901 ggcctgtttt ttttcttcct gagtgatttc cgtattcaga aaggtttcaa tagccagcag
      961 caattcgatg gcgtcaatac tgtcgaattc ataggtttcg cgcaaatcag catccatatc
     1021 cggattgtcg atttcaaatt cccgctgaaa aacgtttttt acttcgcgct caatttcctg
     1081 acgtgtcata gataggactt taactttctg aatgtgatgt tgtgaccttt aacaaatatc
     1141 gtatggcttt tcaatgaaat tcattgcata ttttcgttga tttcccgggc aaatcgttcc
     1201 gcacccgtga tttgccggac cgcgttgaag gcgccgaaaa gacagcccat cacgcccggc
     1261 gccatggcgt tttgtcctgc cagacacaaa ccgggaatcg gcagattatt cagcgaaatg
     1321 gccttcaata actgacgcga cgaacgcatg atcccgtagc aggctccttc cggagcactg
     1381 acataatccc ggagggtgag aggcgtgtat acatccagaa tccgggcatc gttcaaatcg
     1441 ccgaagacgt cttcagcctt tttaagcagg cctttggcga ggttcatttt gtgctcttca
     1501 tactcgctgc cgcgttggcc tgtcacggta ttttcccatc gactccaatc accgaagagg
     1561 gatttggtaa taatcgaaag cagatgaagg cctgcggagg tcgtgcgttg cacttgataa
     1621 aatacgcctt cctgaatcac accgttttca tcacggtgga gcctgtaaat gttatgatgc
     1681 atttcaggat gcttggcggc gtcaacagag acctgaaccg cgataacgct ttccgtttcc
     1741 ttaagtccca gaatgcgccg gcgatgagaa tcgcgcagaa cattttcgtc cagcaattga
     1801 agcatgacct tcgggtgtat agcggcaacc accgcatcgg cgaaaagcgt tctggccgat
     1861 tccaaaatca ctccgcttac tttacccttt tcaaacaaga ttttcttgac gccgtcgtgc
     1921 aggacaagtt gccccccgag gtcttcaaag cggcggataa agacatccgt cattttacgg
     1981 ccgcattcct tcagtcgcca cgaagaaaaa agataactgg ctagcaccat gtgatggaaa
     2041 atgaccggac aatcgttgag cgggacgccg ataagattac agggaaccga taaaacggca
     2101 cgcaaggccg gtgatacgtt caaagaatcc agtaattctc ccatcggctg gtaatagccc
     2161 atattctgaa agggatcacc ctgagcgaac aagaacgagg gatccgtcat ttgccgggaa
     2221 atgtcgcgca gatttttcat aaggacatcc agcgccggtt tttcctgaag gaacgtatct
     2281 ctcagactgt tttccagcgt gtcgattccg tcgggtaaat cgaaggttaa atcgtcgaaa
     2341 atatagcggt cgacgatgcc ttctccagcc atgggaacaa atagctcgtc aaccgggatt
     2401 cccaaggccg caaacatttt accgaggggt tcttccgccc ccaatgcgcc gacgtagtga
     2461 atcccgacag ggcagtctat tccgccgcgc cggtaagacc gcatcaatcc gccgggcagc
     2521 ggattttttt ccacgaccgt gacgtgaaaa ttcatcaaag ataaaataat cccggtactc
     2581 aatccgccga taccggaacc gatgatcacg atgttcttgc catcgacgct atttctcatc
     2641 tgcttgcccc tttttgtttt tctgtatgca tttgttttct ataagtcaac cggaaatgat
     2701 cctttcgtct gccgggggat aaaaacaaat tgacatcggg gaaagttatt tctatacttt
     2761 aaccattcca gaaaaaaagc gcggcacata tccgttgggg ggatttctta tatcaataag
     2821 aaaaggattg ctgcgttcgc ggtcgccatc gttcttggca ttgttgcggc accggttatc
     2881 aaaaacaggt tgacgggcga gcacgcccaa caggattatg tctttgataa aaattccaga
     2941 atcccattaa aagatatcaa aacccattat tagtatgttg atttcgagaa ggaaaaagcc
     3001 gcggagtatt tgaatgatga tgtgaccaat ccctatacga taaaattttt aatgtttctt
     3061 aacagccgtt gaaaatatag aagagaaata catttatgga aaaaataagc gattatgttc
     3121 tggaaaaaaa gatacacaaa acgaaaaatg ccgttacgta ccggggttat aaggaaaaag
     3181 attttcaatc cgttattatt aaactcctga acacccttca ttcaacccct tcggaaattg
     3241 cacaattccg gcaggaatac gaactgattc aacgtcttga tatcggaagc gttgtcaaaa
     3301 ccatcgatat tattcattac aaaggcggcc tggcgctcat cctcgaggat tttgacggta
     3361 ttcccgttaa ggactatttc cgcccgaatg aaaaaatcaa catcgcatca tttctcgccc
     3421 tatcggcgaa cattgccgca acactggcag cgctgcatgc ccggggagtc attcaccgga
     3481 acataaaacc ccaaagcctt ctgatcaatc cggaaacgga ggaattgaaa attacggact
     3541 tcggaatatc cgctctgctg actcacgaac atgatgaagt ctataatcct gatttcatcc
     3601 gcgataccct gacatacatg tcgcctgagc agaccggccg gatgaacagg tctgttgatt
     3661 accgcacgga tctctattcg ctgggaatca ctctgtatga aatcgtcacc ggtttgctgc
     3721 ctttcaagtc gcaggacccc atggaactca ttcatgcgca tatcgccatg atgcccaggg
     3781 cgcccgttaa gcttgatgcc ggtgttcctg ccatggtgtc ggatatcatc atgaggctgc
     3841 tggccaaaaa ccccgaggcc agataccaga acggtttcgg tcttcaagcc gattttcagg
     3901 aatgcttgaa acaatgggaa gagaaaaaaa ggatagaacc ctttgaactg gggagacagg
     3961 atatatccaa ccggttcatc gttccgcaaa aacttttcgg acgggaaaag gaaatatcgg
     4021 aactgatctc cagattcgag gaggtagcca acggcgacaa gagattgtcc gtgatggttg
     4081 ttgccggtgc tcccggtatc ggcaaatcgg ccatgattaa tgaaatccat aaaccgatcg
     4141 tggctaggcg gggctatttt atttccggca aatatgagca attcagaagg gacaaacctt
     4201 acagcgccat tattcaggct tttcaggttc tcgtgaaaca aatattgtcg gaaagcgaac
     4261 aaagaatcaa tctctggaag aaaaatcttt taagtacgct gggcgacaac ggtcgggtga
     4321 tcacggacgt gattccggaa gtcgaatgga tcatcggaaa gcagccggat ttgccggttc
     4381 tgggtccgga agagacccgg aaccggttta aatttatctt tgaaaaattc atgagtgtct
     4441 ttccgcaaca ggaacatccg gtggctcttt ttctggatga tttgcagtgg gcggatatgg
//
LOCUS       DAOOLB010000133         4469 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_23462_length_4469_cov_30.591074, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000133 DAOOLB010000000
VERSION     DAOOLB010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23462_length_4469_cov_30.591074"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="PLF77_10575"
     CDS             complement(<1..945)
                     /locus_tag="PLF77_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HPC09342.1"
                     /translation="MKKRIVITGMGVAAPNAHGLDNFEKALRNGVSGIRFIPRLQELN
                     FGCQIAGVPEYFDEIRSSYFDREKLLSINDNIGYASVSAVDAWTDAGFALKDDDDDTV
                     DWDTGVIAGSGIGGMDTIANTIVPLINEGKVRRLGSRVVEQVMGSGTSARISGLLGVG
                     NQVTSNSSACSTGNEAIIDAMGRIRTGLAKRMIAGGSEGATPYIWGGFDAMRVLCRKF
                     NDNPEAGSRPLSASAAGFVPGAGGAMLVLEELETALGRGARIYAEILGGYVNCGGQRM
                     GGSMTAPNPEGVKRCIRGAVGDAGIDPAEIDAINGHLTA"
     gene            complement(942..1412)
                     /locus_tag="PLF77_10580"
     CDS             complement(942..1412)
                     /locus_tag="PLF77_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hydroxyacyl-ACP dehydratase"
                     /protein_id="HPC09343.1"
                     /translation="MEADAALKQEVLSLVPQQPPFRFIDEIISLDDEQIIGAYRFRED
                     EYFYRGHFPGNPITPGVILIETMAQVGVVAFGIYLLSRQKNMRLHDMKLPISLFSLAD
                     GVEFKQIVKPGERVIVRAKKIYFRKMTIKAAVSMEKENGEMICSGELSGVGVEQ"
     gene            complement(1402..2130)
                     /locus_tag="PLF77_10585"
     CDS             complement(1402..2130)
                     /locus_tag="PLF77_10585"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HPC09344.1"
                     /translation="MIERPVALVTGGAKGIGAACCRALAAEGFHVGIHYNQSTASAQT
                     LRDQIREGFLIQADLSDIGQVDAMIGKLKDTTGRVDVLVNNAGRSVNADIISMKVEQF
                     DEQRTLTRGVWYLTKRILRQFMFRNSAGRIINISSVVGHTGNAGQIPYTMEKAALDAF
                     TRSLARELKGRNILVNSVAPGFIDTEMTKDLPEEIKNKAMEDILLGRVGRPEEIAEVV
                     AFLAKKGSYINGSVIHVNGGLYGG"
     gene            complement(2127..3119)
                     /locus_tag="PLF77_10590"
     CDS             complement(2127..3119)
                     /locus_tag="PLF77_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HPC09345.1"
                     /translation="MLYLHAMGHFHPENVISNQFLEELDMGTSNDWIMERVGIQNRRT
                     VLPLDYIRKTKNVHPLQSFSIRLYTNAQMSAMAARMALQRAGLKAKDIGLIISGSSSP
                     DNITPAEAGAVAAELGIEVPCFDLNSACSTFGMQVNFLLRMKPEEMPPYVLLVGAEST
                     TKVINYTDRNSSVLFGDGAHAAVVSSTIPARAAFISGGYNARPSGWTKVGVTGDWVFS
                     QDGNAVQGFAIRTTTECLKKLQDQYEAQAKRFIFIAHQANYMMLKTVCERRHIKPEHH
                     WFNVDQFGNVGCCGAPAVLSMNWDNIRPGDNIALVIVGAGLAWAYAMLKVEDKQ"
     gene            complement(3119..4318)
                     /locus_tag="PLF77_10595"
     CDS             complement(3119..4318)
                     /locus_tag="PLF77_10595"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPC09346.1"
                     /translation="MDFSLNESQLSYLDTVRKFVKNEITPRILDMEKEHVFPWEIINK
                     AWKTGLINLSIPESVRGYEMDLFTSALVVEGLSYGDTGISTSAMCNDLANVVIGLHGT
                     DEQKNLFLKPFVEKPILASFCLTEPNAGSDNSMMAAFISKREDGKYILNGAKCFITNA
                     SYASQFTVLCKTGKPTGNFMACVIVPVEHVTSYDIPDIGNATREISVPAGGKIIIGKP
                     EDKLGQRLSNTASVTFEDVVVFPNHIIGDRRLGFKYIIDVLDYARPMVAAIGLGLAKR
                     AFDLTVEYTRERKQFGHRICDLPVAREMLTTMWKKVELAEMALFKAIFKIQEKAQDAG
                     IYASLAKNTAAEAAVFCATEGLHLHGGYGFTTEYEISKLARDAHIIDIYEGVREVQNM
                     IIGREII"
BASE COUNT         1076 a         1232 c         1064 g         1097 t
ORIGIN      
        1 tgccgtcaga tgtccgttaa tggcgtcaat ttccgccgga tcaatacccg catcgccgac
       61 agcgccgcga atacatcttt tgacgccttc gggattgggc gcggtcatgg agccgcccat
      121 ccgctggccg ccgcagttca cataaccgcc gaggatttca gcgtagatcc gggcgccccg
      181 gcccagcgcc gtttccagct cttcgagaac aagcatggcg ccgcctgcgc cagggacaaa
      241 accggccgct gaagcactga gcggacggga gccggcctcg gggttgtcat taaattttct
      301 acacagcacg cgcatggcgt cgaacccacc ccagatgtaa ggcgtcgcgc cttcggaacc
      361 gccggcgatc atgcgtttgg ccagtcccgt tctgattctc cccatcgcgt caataatcgc
      421 ctcattacct gtcgagcagg ccgaagaatt cgaggttacc tggttgccga ctcccagaag
      481 gccggaaata cgcgcactgg ttccgcttcc catcacctgt tcgacaacac gactgcccag
      541 tcttcttacc ttaccctcat taattaaagg aacgatggta ttggcaatcg tatccatgcc
      601 gccaattccg gaaccggcaa tcactcccgt atcccaatct acggtatcgt catcgtcatc
      661 cttcagggca aatcccgcat ccgtccaggc atctaccgcc gacaccgagg cataaccgat
      721 attgtcgttg atggacaaaa gtttttcccg gtcgaaatag cttgaccgta tttcatcgaa
      781 atattcaggg acgccggcta tctggcagcc gaaattcaat tcctgtaacc gggggatgaa
      841 gcgaataccg gaaacgccat tgcgaagagc cttttcaaaa ttatccaagc cgtgcgcgtt
      901 gggcgccgcc acccccattc ctgtaatgac aattcgtttt ttcattgttc cactcccact
      961 cctgataatt ccccgctgca gatcatttcg ccgttttcct tttccatgga cacagcggct
     1021 ttaatggtca ttttacgaaa ataaattttt ttcgctctga caataactcg ttcacccggc
     1081 ttcacaatct gtttgaattc cactccgtcg gccagagaaa aaagactgat cggcaatttc
     1141 atgtcatgga gtcgcatatt cttttgacgc gacaaaagat atatcccaaa ggccacgacg
     1201 ccaacctggg ccatcgtttc aatcagaata acacccggcg tgatgggatt gccgggaaaa
     1261 tgcccgcggt aaaaatattc atcttcgcga aaacggtaag ccccgataat ctgctcatcg
     1321 tcaaggctga taatttcatc gataaaacgg aaaggcggct gctgaggaac aagcgaaagc
     1381 acctcctgtt tcagagcggc atcagcctcc ataaagtcct ccgttgacgt ggatgaccga
     1441 cccgttgatg taactaccct tttttgccaa aaaggcgact acttcggcaa tttcctccgg
     1501 acggcctaca cgtcccagga gaatgtcttc catggcttta ttttttattt cttcgggcaa
     1561 gtccttcgtc atctccgtat cgataaaacc gggagccacg gaattcacca gaatatttct
     1621 tcccttgagt tcccgcgcca gcgatctggt gaaggcatcg agcgccgctt tttccatcgt
     1681 gtagggaatc tgtccagcgt tgccggtatg gccgacaacg ctggaaatat taatgatgcg
     1741 gccggcggaa ttccggaaca tgaattgccg caaaattctt ttggtcaggt accagacgcc
     1801 gcgcgtcaac gtccgttgct cgtcaaactg ttccactttc atggagataa tatcggcatt
     1861 gacggagcga ccggcattgt tgaccagaac atcgacacgt cccgtggtat ccttaagctt
     1921 accgatcatc gcgtctacct gcccgatgtc ggataaatcg gcttgaatta aaaaaccttc
     1981 tctaatttga tcccgtaacg tttgagcgct tgcggtactt tgattgtaat gaataccgac
     2041 atgaaaccct tccgcggcca gcgctcggca gcacgcggcg ccaatgcctt ttgctccgcc
     2101 ggtaaccagt gcaacgggcc tttcaatcat tgtttatcct ccaccttgag catcgcatac
     2161 gcccaagcca gtccggcgcc gacaatcacc agggcaatat tatctcccgg ccgtatatta
     2221 tcccagttca tactcaaaac cgcgggagcg ccgcagcagc cgacattgcc gaactgatcc
     2281 acattgaacc agtgatgttc cggtttaatg tgccggcgtt cgcaaacggt tttcagcatc
     2341 atgtaattgg cctggtgcgc aatgaagata aagcgcttgg cctgagcttc gtactgatcc
     2401 tgaagttttt ttaaacattc cgtcgttgtt ctgatggcaa aaccctgaac ggcattgccg
     2461 tcctgactga aaacccagtc tccggtaaca ccgacttttg tccatcccga aggacgggcg
     2521 ttataaccac cgctgataaa ggcggcacgg gcgggaatgg tcgacgaaac aacggcggca
     2581 tgagcgccat cgccgaaaag cactgatgaa ttacgatccg tatagttaat gactttggtc
     2641 gtggattccg caccgaccag aagaacgtaa ggcggcattt cttcaggctt catacgcaac
     2701 aagaaattaa cctgcatgcc gaaggtggaa caggcggaat tcaaatcaaa gcagggtacc
     2761 tcgatcccca gttccgcagc cacggctccg gcttccgcgg gagtaatgtt atccggggat
     2821 gatgaaccgg aaataatcag accgatatcc ttcgctttca atcccgcgcg ctgaagtgcc
     2881 atccgtgccg ccatggctga catctgcgca ttggtgtaga gccggatgga aaaggattgc
     2941 agcggatgga cgtttttggt tttccggata tagtcgagcg gcaaaaccgt gcggcggttc
     3001 tgaatgccca cccgttccat gatccagtcg ttagacgtac ccatatccaa ttcttccaaa
     3061 aactgattgg aaatgacatt ctccggatga aagtgcccca tggcatgaag ataaagcatt
     3121 atataatctc ccgtccgata atcatatttt gaacttcccg gacaccttca tagatatcga
     3181 taatgtgagc atcgcgcgcc agtttggaaa tttcatattc ggttgtaaaa ccataaccgc
     3241 cgtgcagatg caggccttcc gtcgcacaaa agacggcagc ttcggcggcg gtgttcttgg
     3301 ccagggacgc atagatgccg gcatcttggg ctttttcctg aatcttgaaa atggccttga
     3361 acaaagccat ctcggccagc tcgacttttt tccacatcgt cgtcagcatt tctctcgcca
     3421 cgggaaggtc acaaatgcga tgaccgaact gcttgcgttc ccgtgtatat tccaccgtca
     3481 gatcgaacgc tcttttggca agccccaggc cgatggccgc caccatcgga cgggcataat
     3541 ccagaacatc aatgatatat ttgaaaccca gacgccggtc gccgataatg tgattcggaa
     3601 aaacgacaac gtcctcgaac gtcacgctgg cggtgttgga aagccgttgt ccgagcttat
     3661 cctcgggttt accgatgatg attttaccgc cggcgggaac gctgatttca cgggttgcgt
     3721 tgccgatatc ggggatatcg tatgaggtga cgtgttccac cggcacaatg acgcaggcca
     3781 taaaattgcc ggtcggcttg cctgttttgc acaaaacagt aaattgcgat gcgtaagacg
     3841 cgttggtgat aaaacatttg gccccgttca gaatatactt accgtcttcc cgtttgctga
     3901 tgaacgcagc catcatggag ttatccgaac cggcattggg ctccgtcaga caaaaagagg
     3961 ccaggatcgg cttttcgaca aacggtttaa ggaatagatt tttctgctcg tctgtgccgt
     4021 gcagtccgat gacgacgttg gccagatcat tgcacatggc cgacgtagag atgcccgtat
     4081 cgccgtagga aagcccctca acaaccaggg cggacgtaaa cagatccatt tcataaccgc
     4141 ggacggattc cgggatgctc aaattgatca gccccgtttt ccaggccttg ttaatgatct
     4201 cccagggaaa aacgtgctcc ttttccatgt ccagaatacg cggcgtaatt tcattcttca
     4261 caaatttgcg aacagtatcc agataggata actggctctc gttgaggctg aaatccataa
     4321 agcacacctt tacttatttt atttttttgg agatgaactt ataaaacaac tccttttaag
     4381 aatttttcgc ttcaaaaaag gagtttattt cggtttaaat tttttatgcg gccgcgcctt
     4441 ttttctgggc attgtcgtcg cctgtcgcc
//
LOCUS       DAOOLB010000134         4344 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_24342_length_4344_cov_45.214502, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000134 DAOOLB010000000
VERSION     DAOOLB010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24342_length_4344_cov_45.214502"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="PLF77_10600"
     CDS             complement(<1..478)
                     /locus_tag="PLF77_10600"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-coenzyme A synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HPC09347.1"
                     /translation="MAEANEQDFYDKVFPVPDRVREKSYIKSRAEYDKLYKESITDPN
                     AFWAKMANERLTWFKPFDKKKVSDWSFDAKDVHSKWFEGGKINVSYNCLDRHLATKKN
                     KAAIIFEGNDPNDWKVYTYYDMYREVNKFANVLKGLGVKKGDRVTIFLPMIAELSIV"
     gene            complement(1099..1866)
                     /locus_tag="PLF77_10605"
     CDS             complement(1099..1866)
                     /locus_tag="PLF77_10605"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HPC09348.1"
                     /translation="MNHQDVVAFVTGGASGLGEASVLELIKKGAAVVIADVDTEKGES
                     LAAGIGSKAFFVKADVTSETEIRQAIEKAVEIFGKINVVVNCAGIVSPGKLVGRKGPL
                     PLEAFNKVLQINLVGTLNVIRLAVEQMMKNTPGEEGEKGVIINSASIAAFDGQVGQVS
                     YSASKAGIVGMTLPIAREISEYGLRIVTVAPGIFDTPMMAGLPPTVKEGMAQMVPFPK
                     RLGKPVEFARMVLHIIENTMLNGCCIRLDGALRMPPK"
     assembly_gap    1890..1989
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2072..3451)
                     /locus_tag="PLF77_10610"
     CDS             complement(2072..3451)
                     /locus_tag="PLF77_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HPC09349.1"
                     /translation="MNIAVLNGSPKGDVSVTMQYVHFLQQRFPETEFRIFDVALKIKK
                     LENDDRAFQEVIANVADADAVLWAFPLYFLLVCSQYKRFIELIFEKNAVSAFHGKPAA
                     CLTTSINFFDYTAHNYIHAVSDDLGMNYFGSYSAEMNDLLKTKERRRLKTFGELFLKA
                     IREKTPAIRSYKPLVAPEIVFDTEAIRPVDAAGKKVLILADDLAANSNLRKMVERLQK
                     TFSPAAELVQLKDVDIKGGCLGCIKCGFNNVCVYEGKDGYIDFFNHKIKPADIVFLAG
                     AIHDRYLSSLWKCFFDRSFFNGHTPFLKDKQIGFVISGPFGQIHNLREIFDATTKFQL
                     AGLAGFVSDDTGGNPEINAALQTLAVQAAEQSRLGYIQPPTFYQVAGAKLFRDVIYGR
                     LRPVFQADHRYYKEHGMYNFPQKYYKWRMINSTLVFLTKIPAFRREFFKRIKKEMIKP
                     AAGALKKTE"
     gene            complement(3565..3759)
                     /locus_tag="PLF77_10615"
     CDS             complement(3565..3759)
                     /locus_tag="PLF77_10615"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HPC09350.1"
                     /translation="MEQNMGTVDKVIRTIVAILIIVAYLMGMLPGIAGLLLIISGSLL
                     SSVVSGYCPLYTKIGVNTNR"
     gene            complement(3912..4283)
                     /locus_tag="PLF77_10620"
     CDS             complement(3912..4283)
                     /locus_tag="PLF77_10620"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPC09351.1"
                     /translation="MNEKRKASRLMEENEVTITLIAGGKKFFREKVIYNSSKDISESG
                     ARVHSSIFLPIDTLLKIDFKLKNLQHMITAMGKVKWIKIICEGEAYDAGVEFVNTPTE
                     AIRKIADYIAWRQKQKTTKRH"
BASE COUNT         1044 a         1070 c          911 g         1219 t
ORIGIN      
        1 tgacgatgga cagctccgcg atcatgggca ggaagatggt aacgcggtcg ccctttttga
       61 cgcccaagcc cttcaggaca ttggcgaatt tgttgacttc gcgatacatg tcgtaatagg
      121 tgtatacttt ccagtcgttg ggatcgttgc cttcgaaaat gatcgcggct ttgtttttct
      181 tggttgccag atgacggtcc agacagttgt agctgacatt gatcttgccg ccttcaaacc
      241 attttgaatg aacgtctttt gcgtcgaagg accagtccga cacttttttc ttgtcaaaag
      301 gtttaaacca ggtcagtctt tcattggcca ttttggccca aaatgcattg ggatcggtga
      361 tcgattcttt gtacagtttg tcgtattccg ctctgctctt gatgtaagat ttttctctta
      421 ctctgtcagg tacgggaaat accttgtcat aaaaatcttg ttcgtttgct tcagccatat
      481 tattctcctt tatctaaatt atttttcatg cctttcgctt ccgcctaaaa agcattttta
      541 aatccgtatc gtcttccttt ttttgacctc gttttaaccc ggtcttctca acttttcagg
      601 aggggtacgc cccaggcttt ggcctcgttc ctgttagaaa aaaacgttca tgattcaacg
      661 gccgcaaggt ggggcgtatt acatggctga tgcaatacat ataccccctg aaaagcgatt
      721 actaaataca ccttttgcaa gggttgcggt atcggcttga gtcctaatta aaagcgggat
      781 tttatcctac gggatgtaag tgtttgtcct acagattctt tccgataaag aacaaatcag
      841 aaagcaaatg cacgatggcg cgcatggata gaagaaatca tgacatgctt cttatcctgc
      901 aaatcaggtt gataaatctt attgaatttc aaaagtcgca ttttatctgt ttggtttcct
      961 ggacgaaaac ggattcttct ttccggtgcg ccgtaaagga ctgaactttt tttcgcgcag
     1021 aagcctggaa atatcttttt gccttcgggt cgttttctgc ccggccataa aaattatctt
     1081 ctatgtgttc tatcctgatc atttgggtgg catgcgcagc gccccgtcca gccggatgca
     1141 acagccgttg agcatcgtgt tctcaatgat atgcaacacc atgcgggcaa attccaccgg
     1201 tttacccaaa cgtttcggga aaggaaccat ttgggccata ccttccttga ccgtcggggg
     1261 caatccggcc atcatcggcg tatcgaaaat tcccggtgcc acggtaacaa tccttaatcc
     1321 gtattcggaa atttcccgtg cgataggcaa cgtcataccc acgatccctg ccttggatgc
     1381 gctgtaggag acctgcccca cctgcccatc aaaggccgcg atcgacgcag agttgatgat
     1441 cacgcctttt tctccttctt caccgggagt atttttcatc atctgctcca ccgccagcct
     1501 gatgacattg agcgtcccga ccagattaat ttgcaaaacc ttgttaaacg cttccagggg
     1561 cagaggccct tttcggccga ccagtttccc cggactgaca atgccggcac agttcaccac
     1621 aacatttatt ttcccgaaaa tctcaacagc tttttctatg gcctgcctga tctctgtttc
     1681 gctggtgaca tcggctttca caaaaaaagc tttgctgcca atacccgccg ccaggctttc
     1741 gcctttttcc gtatcaacat cggcgataac aacggcagcg ccttttttaa tcagttccag
     1801 aacactggcc tcgcccagcc ccgaagcgcc tcccgtcacg aacgccacga catcctgatg
     1861 attcatgttt cctccagagt tttattgaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnng tcacgaacgc cacgacatcc tgatgattca tgtttcctcc agagttttat
     2041 tgaaatatat gcttttcaga tgcaacaacc atcattctgt tttcttcaac gcgccggctg
     2101 cgggttttat catttccttt tttattcttt tgaagaactc gcggcgaaac gccggaattt
     2161 ttgttaggaa aaccagcgta ctgttgatca ttcgccattt gtaatatttt tgaggaaaat
     2221 tgtacatccc gtgttccttg tagtaccggt gatcggcctg aaaaacaggg cgcaacctgc
     2281 cgtaaatgac atcccggaat aatttcgcgc cggcaacctg ataaaatgtc ggcggttgaa
     2341 tataaccgag ccggctctgc tcagccgcct ggacggccag agtctgcaag gctgcgttga
     2401 tttccggatt tccgcccgtg tcatcgctga caaagccggc aagaccggcc agttgaaatt
     2461 tcgtggtcgc atcaaatatt tcacgcaaat tatgaatctg tccgaacgga ccggaaatga
     2521 caaatccgat ctgcttgtct ttcagaaacg gtgtatgccc gttaaaaaaa ctgcggtcaa
     2581 aaaagcattt ccacagcgaa gacagataac ggtcatgaat ggcgccggcc agaaacacaa
     2641 tatcggccgg cttaattttg tgattgaaaa aatcgatgta accgtccttg ccctcgtaaa
     2701 cacaaacatt attgaaaccg cattttatac agccgaggca tccgcctttg atatcaacgt
     2761 ccttgagttg caccagttcg gcggcaggag aaaaggtttt ctgcaatcgc tccaccatct
     2821 tccggagatt ggaatttgcc gcaagatcat ctgccagaat caaaactttt ttaccggccg
     2881 catccacagg gcgaatcgct tcggtgtcaa aaacgatttc cggagcgacc agcggcttgt
     2941 agcttctgat tgccggcgtt ttttctctga ttgcttttag aaacaattca ccgaacgttt
     3001 tcaggcggcg gcgctccttg gttttcagca ggtcattcat ttccgctgaa taggaaccga
     3061 aataattcat tcccagatcg tcgctgacgg catgaatata attatgggcc gtatagtcga
     3121 agaaattgat ggaggttgtc agacaggccg ccggcttgcc gtgaaaagcg gaaacggcat
     3181 ttttttcaaa aatcaattcg atgaatcttt tatactgcga acagaccaga agaaaataaa
     3241 ggggaaacgc ccagagcaca gcatccgcat ccgccacgtt cgcaatgacc tcctgaaacg
     3301 cccggtcgtc gttttccagt tttttaattt tcaatgccac atcaaaaatt ctaaattccg
     3361 tttccggaaa tctttgctga aggaaatgga catactgcat ggtcacgctg acgtcacctt
     3421 ttggcgaacc gtttaaaacg gcaatgttca tattatcacc ttttattcca tatgggattc
     3481 atcgggactc ttatcacagg gagggtctcg gctcaagaaa atacccggta aaacagcttg
     3541 cgcaatcgat tgcaaacagg cggattaccg gttcgtgttc actccaatct ttgtataaag
     3601 cgggcagtat ccgcttacca cactggagag aagcgatccg ctgatgatga gcagcagccc
     3661 cgcgattccc ggcagcatac ccataagata ggcgacaata atgagaatcg ccacaatcgt
     3721 tctgatcact ttgtcaacgg tacccatatt ctgttccatg atccctcctg atgatgattt
     3781 ctcttcttcc ggacatttca agatgccgga atcaaccatc tgccgttttc acaccaaacc
     3841 gatgccgcac attacacttt cacggcaaga ccgccttccg gaacgtattc aaacaacggg
     3901 agccaatagg gtcaatgacg ttttgtggtt ttctgctttt gccgccatgc aatgtaatcc
     3961 gctatttttc tgatggcttc cgtcggcgtg ttcacgaatt ccaccccggc atcatatgct
     4021 tcgccctcac agataatttt gatccacttc accttaccca tggcggtaat catgtgttga
     4081 aggtttttta atttaaaatc tatcttgagg agagtgtcga tcggcaaaaa tatgctggag
     4141 tgaaccctgg cgcctgattc cgaaatatcc ttgctgctgt tataaatgac tttttctctg
     4201 aaaaattttt tgccaccggc aatcagggtg attgttacct cattttcttc catcaggcgg
     4261 ctggcttttc ttttttcgtt cataacaatc cgttactttt gggtaatcgt ttccgggtta
     4321 attacacttt cgagaaccga aaaa
//
LOCUS       DAOOLB010000135         4333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_24426_length_4333_cov_52.351332, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000135 DAOOLB010000000
VERSION     DAOOLB010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24426_length_4333_cov_52.351332"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            668..765
                     /gene="ffs"
                     /locus_tag="PLF77_10625"
     ncRNA           668..765
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLF77_10625"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            917..>2084
                     /gene="dnaX"
                     /locus_tag="PLF77_10630"
     CDS             917..>2084
                     /gene="dnaX"
                     /locus_tag="PLF77_10630"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPC09352.1"
                     /translation="MEYQVLARKFRPQTFEEVAGQAHVVKTLRNAIAQGRVAHAFLFS
                     GPRGVGKTSVARILSKALNCEKGPTPTPCNQCSNCIEITNNSSLDVHEIDGASNRGID
                     EIRELRENVKFAPASSRYKIYIIDEVHMLTDPAFNALLKTLEEPPAHVVFIFATTESH
                     KIPATILSRCQCYDFRRIPLKEIIANLELVASKEGINISPIALSWIAEAGDGSMRDSQ
                     SIFDQVISYAGLDVKDADVEESLGLAARQYLFRLSEAVLNQDAGSCLIILEEAYLAGI
                     DMKYFYQMLLKHFRNLLLVKIAGDKSASFDIATEQIEKLNTQVQTTSRETLQRYLEIL
                     IAEEGNFRRSQEPRMKLETIIVKMAYLEPIMPLGEILSTIETIEKKLQSGLSGND"
     assembly_gap    2085..2184
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2185..2769
                     /locus_tag="PLF77_10635"
     CDS             <2185..2769
                     /locus_tag="PLF77_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09353.1"
                     /translation="STIETIEKKLQSGLSGNDEKTVPQSIAAQGSSGGMCSAGAANSF
                     SGECKDENHCPPDNAADLKGLRDNLKKFIKKESPILAAKIESAEILRCENNCLTIGFP
                     ANFIFLDEMKNVQKQRLEQIAGKFFQQNMTLVIETLSAENGGANGGRPRSKAGILNDI
                     KQEAMKQPLLRKIIDELPDARVIDVRVQTEKTKS"
     gene            2813..3121
                     /locus_tag="PLF77_10640"
     CDS             2813..3121
                     /locus_tag="PLF77_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002107277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HPC09354.1"
                     /translation="MQNFNTIMKQAKKMQERMASLQKELETKTVEAQAGGGMVSVVVN
                     GKYEIVSLKIEKEVVNPEDIEMLQDLIAAAVNEGVRKSQEMAAAEMGKITGGLNIPGF
                     "
     gene            3135..3734
                     /gene="recR"
                     /locus_tag="PLF77_10645"
     CDS             3135..3734
                     /gene="recR"
                     /locus_tag="PLF77_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPC09355.1"
                     /translation="MKAYAQPIKKLIAELGKLPGIGEKTATRLANYILRSTEDDVIKL
                     AQSIVEVKKKIKLCRICLNLTEDDTCHICRDASRDQEVICVVEDPDALAAIEESGGFK
                     GIYHVLHGALAPLDGIGPDNLRLGELVQRINRGNIKEIILATNTNVQGESTALLLTEM
                     FKEKNIIITRIAMGMPMGGDLKYIDKMTISKSLEYRRGM"
     gene            4017..>4333
                     /locus_tag="PLF77_10650"
     CDS             4017..>4333
                     /locus_tag="PLF77_10650"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF017493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPC09356.1"
                     /translation="MATEITTKTKLRKINAKKITKAVKKLFIEANTVLGEDVVEALHQ
                     ALSREESSSGRQVLQKIIENADIARRKKMPICQDTGLAVVFVELGQDVHITGMPLHAA
                     IEEG"
BASE COUNT         1228 a          947 c         1097 g          961 t
ORIGIN      
        1 gtgtgtacgc ccccaaaggt gtaccgcggg ttcaaatccc gctctctccg ccattatttt
       61 gtgaaactcc aattccgaaa gcgaaacgca acggaattgg taagttgaac aatcccacat
      121 aaatgtaggg aaatgagacc caaatttcag aaacaaagtg cactgaaatt tgatagtgga
      181 attatccctt gtgcgaagca catagggata tcccgcctaa gcaggaataa tatagcattc
      241 gtgatttatc aatcttctgt aaaaacaata acatcgtttg tgaaatctag acgcaaaaaa
      301 tttgagttga ttcctgttcg tttatctatt aaaagtctca gcaggttgtt tatttaaaaa
      361 tatggccggg tcccgcgcaa cggaggtagt gagactccaa ttgcgcaagc gaagcgcggt
      421 ggaattggta agtcgaacta tctccaaagc gctgagacta atgcccggag tgacaaagag
      481 agattttgtt tcttttcatt atatgaaaat aatatggccg ggtcccgcgc aacggaggta
      541 gtgagactcc aattgcgcaa gcgaagcgcg gtggaattgg taagtcgaac tatctccaaa
      601 gcgctgagac taatgcccgg agtgacaaag agagattttg tttcttttca ttatatgaaa
      661 ataatatggc cgggtcccgc gcaacggagg cttgtgaacc ccgtcaggtc cggaaggaag
      721 cagcggtaac aatgtccgcc atgtgttgcg gtaaacccgg tcaccagaat accaatctgt
      781 tttatgctgc attaaagttc cctcggggaa aacggacaag atgatgtaat gagatcgcgc
      841 accccacccc caagtcccct cgaccctgaa gggcaccgcg gtctcaaggg gcggggaaat
      901 tatttcggag gtttcattgg aatatcaggt attagcccga aaatttaggc cgcaaacttt
      961 tgaagaagtt gccggtcagg cgcatgtggt caaaacgctg cgcaatgcca ttgcccaggg
     1021 gcgtgtggcc catgcttttc ttttcagcgg tccgcgcggt gtcgggaaaa catcggtggc
     1081 ccggattctg tccaaggcgc tcaactgcga gaaaggaccg acacccacgc cctgcaacca
     1141 atgctctaac tgtatcgaaa taaccaataa ttcttcgctg gatgttcatg aaatcgatgg
     1201 cgcatccaac cgcggtattg atgagatccg cgaactgcgg gaaaatgtca agttcgcgcc
     1261 ggcgtcatcc cgatataaaa tttacatcat tgatgaagtc catatgctaa ccgatccggc
     1321 cttcaatgcg ctcctgaaaa cactggaaga gcctccggcg cacgtcgttt ttatttttgc
     1381 cacgaccgag agccacaaaa ttccggccac gattctttcc cgttgtcagt gctacgattt
     1441 tcgccgcata ccgctgaaag aaatcattgc caatctggaa ctggttgctt ccaaggaagg
     1501 catcaatatc agtccgattg ctctttcctg gattgccgaa gcgggcgacg gcagcatgcg
     1561 tgactcgcag agtattttcg atcaggtgat ttcctacgca ggactggatg tcaaagatgc
     1621 cgatgtcgag gaaagcctgg ggcttgccgc acggcaatac cttttcagat tgtccgaagc
     1681 tgtcttgaat caggatgccg gttcgtgcct catcattctg gaggaagcct atctggccgg
     1741 catcgatatg aagtatttct accagatgct tctgaagcac ttccggaatc tgttgctggt
     1801 caaaattgcc ggcgataaaa gcgcgtcgtt tgatattgcg accgagcaga ttgaaaaatt
     1861 gaacacgcag gtgcagacaa catcgcggga aactcttcag cggtatctgg aaattctgat
     1921 tgcggaagag gggaatttcc ggcgcagtca ggaaccgcgc atgaaactgg aaacgatcat
     1981 cgtcaaaatg gcgtatctgg aaccgatcat gccgctgggt gaaattcttt ccaccatcga
     2041 gaccatcgaa aaaaaattac agagcggctt gtcgggaaac gatgnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnn nnnntccacc atcgagacca tcgaaaaaaa attacagagc
     2221 ggcttgtcgg gaaacgatga aaagacggta ccgcaatcta ttgcagcgca gggctcatcc
     2281 ggcgggatgt gctctgccgg ggcggcgaac agcttttccg gagaatgcaa ggatgaaaat
     2341 cattgtccgc ccgacaatgc ggctgatttg aaaggactgc gcgacaatct gaaaaagttt
     2401 atcaagaaag aaagtccgat tctggcggca aaaatcgagt ccgctgaaat cctccgttgc
     2461 gaaaacaact gtctgacaat cggcttcccc gcgaatttta ttttcctgga tgaaatgaaa
     2521 aacgttcaga agcagcgact ggagcagatt gccgggaaat tttttcaaca aaatatgacg
     2581 cttgttattg agacactgag tgcagaaaac ggtggagcaa atggcggcag accgcgcagc
     2641 aaagccggta tccttaacga tatcaagcag gaagcgatga aacagccgct tttgcgaaaa
     2701 atcattgatg aacttcctga tgcccgggtg attgatgtca gagtgcagac agaaaaaaca
     2761 aagtcgtaaa aaccgaatga aaaatattca ttaaccatga ggaggatgaa gcgtgcagaa
     2821 ttttaacacc atcatgaagc aggccaaaaa aatgcaggag cgtatggcca gccttcagaa
     2881 agaactggag acaaaaaccg ttgaggcgca ggccggcggc ggcatggtca gcgttgtggt
     2941 caacggcaaa tatgaaattg tttcgttgaa aattgaaaag gaagttgtca accccgaaga
     3001 tatcgagatg cttcaggatt tgattgccgc tgcagtgaac gagggcgtcc gcaaatcgca
     3061 ggaaatggcc gcggcggaga tggggaaaat taccggaggg cttaatatcc ccggatttta
     3121 aataaggtac atttatgaaa gcatatgcac aaccgattaa aaagctgatt gccgaactgg
     3181 gcaaacttcc cggcatcggc gagaaaacgg ccacgcggct ggccaattat attttgcgct
     3241 ccaccgaaga tgacgtgata aaactggctc agagcattgt ggaagtcaaa aaaaagatca
     3301 aactttgccg gatttgcctg aatctgacgg aagacgatac atgccacatc tgccgcgatg
     3361 cctccagaga tcaggaagtg atctgtgtgg tggaggatcc ggacgcgctg gcggccattg
     3421 aagaaagcgg cggttttaag ggaatttatc atgttctgca tggggctctg gcgccgctgg
     3481 acggaatcgg gccggacaac ttaaggttgg gcgaactggt tcagcgcatt aacagaggta
     3541 atatcaagga aattattctg gcaacaaaca ccaatgttca gggagagtcc acggcattgc
     3601 ttttgaccga aatgttcaaa gaaaaaaata taatcataac gcgcatcgcc atgggcatgc
     3661 cgatgggcgg cgatctgaag tatattgata agatgacgat atcgaagtcg ctggagtata
     3721 gacgcggcat gtagcatgac gcgtgaaaaa gtccggatac tgcgttgcgc ttcggcatcg
     3781 cgacgtacgc taaaagtacg cctcacttcc aatgcatcgc gtgccttgtc tctggaaatt
     3841 ttttgacgcg tcatcaaata atgtcatact ggcacaggcc ggtatccaga aattgacctg
     3901 ttagaaacgg gaatccgttt caaagagact ggattccgta tcaaggacgg aatgacaaaa
     3961 gagcggcaat catcgatttc ctaaaagtta aaaagtgtca taagggaata aggtttatgg
     4021 ccactgaaat tacaaccaaa acaaaattgc ggaagattaa cgccaagaaa atcaccaaag
     4081 cagtgaagaa gctttttatc gaagccaata ccgttttggg cgaagatgtt gttgaagcgc
     4141 tgcatcaggc actctcccgt gaggaatctt caagcggcag acaggttctg caaaaaatca
     4201 tcgagaacgc cgatatcgcc cgccgcaaaa aaatgcccat ttgtcaggac acaggtcttg
     4261 ccgttgtttt tgtggaattg gggcaggatg ttcacattac cggtatgccg ttgcatgcgg
     4321 cgattgaaga agg
//
LOCUS       DAOOLB010000136         4190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_25519_length_4190_cov_45.204595, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000136 DAOOLB010000000
VERSION     DAOOLB010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25519_length_4190_cov_45.204595"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..2134
                     /locus_tag="PLF77_10655"
     CDS             68..2134
                     /locus_tag="PLF77_10655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPC09357.1"
                     /translation="MKTNRLINEKSPYLLQHAKNPVDWYPWGKEAFEKARREDKPVFL
                     SIGYSTCHWCHVMERESFEDDDVAGLLNETFVNIKVDREERPDIDAVYMTVCQVMTQS
                     GGWPLTIMMTPDQEPFFAATYIPRTTRYGRAGMLQIVPRIKEVWKTKRADVLSAASEI
                     TGILNQSATDKGNRLSEETLRQANRSLSMRFDKTFGGFGAAPKFPSPHHFFFLLRYWK
                     RTGDADALHMAEHTLRNMRSGGIYDHVGFGFHRYSTDERWVVPHFEKMLYDQALMAMA
                     FIELFQATGKTEYEATVREIFTYVLRDLTDEQGGFYCAEDADSEGVEGKFYLWTEKEI
                     RGILNEKEADLFLSLYRHPEDGDLPGMHEIPAGCFIPHLTPSAGDKKDLSGLLSSTEH
                     IREKLFSAREKRIHPHKDDKILADWNGLMIAALARGAQVLDEPVYAKAACRAMDFILE
                     SMQTGDGKLLHRYRSGEAAIDANVDDYAFVIRALLELYEATFQTQYLQKALEYNDDLF
                     SNFLDQKNGGLFFTSLKAERLLTRPKELYDGAVPSGNSVAYGNVLKMSRMTGNADMEA
                     KADAICKAFEAPAGAMPEAFTQFLCGLDFAAGPSGQVIITGDLGKPDTREMLRTLRKQ
                     FAPNNVVIFRPEKPVQPDIETIAPFVKAHLPLNGKATAFVCTDFACHLPTNDPQKMLE
                     LLDTRK"
     gene            2250..2639
                     /locus_tag="PLF77_10660"
     CDS             2250..2639
                     /locus_tag="PLF77_10660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09358.1"
                     /translation="MKKWLISIMVIAFTLSLAGCNFPNFIKKQSPLQEQCNKQCEAWS
                     KSYMQKYPSDKLTYESHYNKRLNKCFMLVTYSKSQLKSLKEISENKMYGSLLVKQNSK
                     TLICNVLENKCKTEKEWDALVKPYMEE"
     assembly_gap    2931..3030
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3031..3467
                     /locus_tag="PLF77_10665"
     CDS             <3031..3467
                     /locus_tag="PLF77_10665"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HPC09359.1"
                     /translation="HQLGVQDIGLVGASLGGYVTGLYVCLSSLPEFAALMVPAVTFLR
                     PISPALFYNQAPYGPQWRKQVYHAADFHSPLHFYPKIPAEKILVVASRGDRICPADPV
                     EQLRIKWGLSRCHYRTGGHWLVFDKIRGRVWYGLLRDMGFIA"
     gene            3613..3918
                     /locus_tag="PLF77_10670"
     CDS             3613..3918
                     /locus_tag="PLF77_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HPC09360.1"
                     /translation="MKWYVTVARIRKECCLRVFILILCFFAAFLTSANAAELYRCVDQ
                     NGNVIVTDRPQAGMENCITNDADEEDSPADNKTDEENASADRPENNQEASDHQAAEP"
     gene            4006..>4190
                     /locus_tag="PLF77_10675"
     CDS             4006..>4190
                     /locus_tag="PLF77_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09361.1"
                     /translation="MSETVITEKDRKMAQRCVECPVCKKARKEQRGFAYWFVKNVEDG
                     LCPNCAAYERVYGKKAH"
BASE COUNT         1092 a          983 c         1071 g          944 t
ORIGIN      
        1 aaagacgcga ttgaaaaagc attttccgcg gcgctgaaat aaatcgaagc aagtttctgg
       61 cacaaaaatg aaaaccaacc gcctgatcaa cgaaaaaagt ccttatctgc tgcagcacgc
      121 aaaaaatccg gtggactggt acccgtgggg aaaagaagca tttgaaaaag cccggcggga
      181 agacaagccg gtttttcttt ccatcggcta ttccacctgc cactggtgtc atgtcatgga
      241 acgagaatct ttcgaggatg acgacgtcgc cgggctgctg aacgagacgt ttgtgaatat
      301 caaggttgac cgggaagagc gtcccgacat tgacgcggtc tacatgaccg tctgccaggt
      361 catgacgcaa agcggcggct ggcccctgac gatcatgatg acgccggatc aagaaccgtt
      421 ttttgccgca acctacattc ccagaacaac cagatacgga cgcgccggaa tgcttcagat
      481 cgttcccaga ataaaagagg tctggaaaac aaaacgcgcc gatgttcttt ccgccgcgtc
      541 ggaaattacc ggcattctga atcaatccgc caccgacaag ggcaatcgac tgtcggaaga
      601 aacgctgcgg caggctaacc ggtctttgtc tatgcgtttt gacaaaacat tcggcggctt
      661 cggcgcggct ccgaaatttc cctcgccgca tcatttcttt tttctgctgc gctactggaa
      721 gcgcaccggc gacgcagacg cgctgcatat ggccgagcat acgctccgaa acatgaggag
      781 cggcgggatt tacgatcatg tcgggttcgg tttccatcgc tattcgaccg acgaacgctg
      841 ggtggtgccg cattttgaaa agatgctcta cgatcaggcg ctgatggcca tggcctttat
      901 cgaactgttt caggcgacgg gaaaaacgga atatgaagca acggtccgcg agatttttac
      961 ctacgtctta cgtgatttga cggatgaaca gggcgggttt tactgcgctg aagacgccga
     1021 cagcgaaggc gtggaaggaa aattttatct gtggacggaa aaggaaatcc gcggcatcct
     1081 caacgaaaaa gaagccgact tgttcctgtc actttatcgc catcctgaag acggcgattt
     1141 gccgggcatg catgaaattc ccgccggttg ttttatcccc catctgacgc cgtccgccgg
     1201 ggataaaaag gatttatcgg gactgctttc ttcaacggag cacatccgcg aaaaactttt
     1261 ttccgcgcgg gaaaaacgca ttcatccgca caaggatgac aaaatattag ccgactggaa
     1321 cggcctgatg attgcggcgc tggcgcgcgg cgcccaggtc ttggatgaac ccgtttacgc
     1381 taaagctgcc tgccgggcga tggatttcat tctggaatcc atgcagaccg gggacgggaa
     1441 actcctgcac cgttaccgca gcggcgaggc cgcgattgat gccaatgtag acgactacgc
     1501 tttcgtcatc cgggcgttgc tggaacttta tgaggcgacg tttcagacgc aatatctgca
     1561 aaaagctctt gagtataacg atgatctgtt tagtaatttt cttgatcaaa aaaacggcgg
     1621 attatttttt acttccctga aggcggaaag gcttttgacc cggccgaagg aactttatga
     1681 cggcgccgtc ccgtccggca attccgtggc ctacggcaat gtgctgaaaa tgtcccggat
     1741 gaccggcaat gccgatatgg aggcaaaggc ggatgcaatt tgtaaagctt ttgaagcccc
     1801 ggcgggtgcg atgcctgagg ccttcacaca atttctctgc ggtctggatt ttgcggcggg
     1861 accttccggc caggtcatca ttaccggaga tttgggaaaa ccggataccc gggaaatgct
     1921 gcggactttg agaaaacaat ttgccccgaa caatgtcgtt attttccggc cggaaaaacc
     1981 ggtgcaaccg gatattgaaa ccatcgcgcc gtttgtgaaa gcccatttac cgctcaacgg
     2041 gaaagcaacc gctttcgtct gcaccgactt cgcctgccat ctgccgacga acgatccgca
     2101 aaaaatgctt gaacttttgg atacgagaaa atagtattgt tttgtatttt ccgtttgccg
     2161 gcgcttctca aaaccaagcg ttaaaaaagc gcctcaaagc agagcaatcc gaaattccat
     2221 gccggtcacg gttcttcagg aggcacatca tgaaaaaatg gttgatatct attatggtca
     2281 ttgcgtttac actatcactg gccgggtgca attttcccaa tttcataaaa aaacaatctc
     2341 cgttacagga gcaatgtaac aaacagtgcg aggcgtggtc aaaatcttac atgcagaaat
     2401 acccgagcga caaattaacc tatgaaagcc attacaataa gcgattgaat aaatgtttta
     2461 tgctggtaac ctactcaaaa tctcagttga aaagtttgaa agagatcagt gagaataaaa
     2521 tgtacgggtc cctccttgtc aaacagaaca gtaaaacgct tatttgcaac gttctggaaa
     2581 ataaatgtaa aacggaaaag gaatgggacg cgcttgtaaa accttatatg gaagaatagt
     2641 cgtgcaacaa aaaacggcat gataaaaaag tcgattttaa acaacgacct cttttttcat
     2701 cgaatttccg tcgacacaac atcatgattg atattccatt gacaggggaa attcttgttt
     2761 atatattcgc gtatgcgaat gaacggattg gatttattag gcagtatggt tgacgagatg
     2821 tttctggcgc cgggccggtt tattgcgtgg ctgcaggctc gccggaaaac agactggccg
     2881 gccttcgccc gtgaaattgc cttttatcac gagcagggat tcgtcaaaaa nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tgcatcaact gggtgttcag gacatcggcc
     3061 tggtcggcgc cagtctgggc gggtatgtca cgggattgta tgtctgtttg agcagtctgc
     3121 ctgaatttgc agccctgatg gtgccggcgg tgacatttct gcgacccatt tctccggccc
     3181 tgttttataa tcaggcgccg tacggtccac agtggcgcaa acaggtttat cacgccgccg
     3241 attttcattc gccgcttcat ttttatccga agatcccggc tgaaaaaata ctggtagtgg
     3301 catcgcgcgg cgaccggatt tgtcccgccg atccggtgga acaactgaga attaaatggg
     3361 gcttgtcgcg ctgtcattac cggaccggcg ggcactggct ggtattcgac aaaatccgcg
     3421 gccgggtctg gtacggtctg ctccgggaca tgggatttat tgcctgaaca gcgagccggt
     3481 tgtgccggct atgaatcagg ccaataaagg ggcttgtttt tcttccggat aagaattaaa
     3541 aacaaagcat caccagagaa gacgaagggg aagatacttc ccgaaaaagg atcttacgga
     3601 gaaaatccgg ctgtgaaatg gtacgttacc gttgcccgga tccggaagga gtgttgcctg
     3661 cgagtattca ttttaatcct gtgtttcttc gcggctttcc taacctccgc caacgccgca
     3721 gagctgtaca gatgcgttga ccaaaacggc aacgtcatcg tcacagaccg tccgcaggct
     3781 ggaatggaaa actgcattac gaatgatgct gatgaagaag attcgcctgc ggacaataaa
     3841 acagacgagg agaacgcctc ggcggaccgg ccggaaaata atcaggaagc gtcggatcat
     3901 caggccgcag aaccttgagg ccggtaaaat acgcaggtgc tcagacagtg gacaaaatca
     3961 tgacgttatt atcaaatgaa gtaaatcttt tttccggagg ccgatatgag tgaaaccgtg
     4021 attaccgaga aagaccgaaa gatggcgcaa cgttgtgtcg aatgcccggt gtgcaaaaag
     4081 gccagaaaag agcagcgggg attcgcctac tggtttgtta aaaatgtcga agacggcctg
     4141 tgcccgaact gtgccgcata tgaaagagtt tacggcaaaa aagcccacga
//
LOCUS       DAOOLB010000137         4078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_26461_length_4078_cov_52.448173, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000137 DAOOLB010000000
VERSION     DAOOLB010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26461_length_4078_cov_52.448173"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..518
                     /locus_tag="PLF77_10680"
     CDS             12..518
                     /locus_tag="PLF77_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HPC09362.1"
                     /translation="MVYAALFGAMTAAGALIVIPLPPVPVTAQTFFMNVAALLLGGPL
                     AVVSQIIYVLLGVVGMPVFAGGKAGLGILFGPTGGYLFGFIIAAFVIGHVHRMKKNAG
                     AVWQVFSMVLGMLVIYVAGSIQLAWIANMSLPQVLIVGVVPFIPGDLIKIALAVLISS
                     RLKNRIEI"
     gene            529..1362
                     /locus_tag="PLF77_10685"
     CDS             529..1362
                     /locus_tag="PLF77_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC09363.1"
                     /translation="MIVLEDVYCRYEPEGIEALRGVSLQIREGERLALIGPNGCGKTT
                     LIRHCNALLVPEKGHVTVGGLRTDHKENQPDIRSRVGMVFQNPDHQIVGMTVEDDVAF
                     GPENLRLPAAKIRARIAESLSRVGIYGLAARNIEDLSGGEKRLVAIAGVLAMRPRYIA
                     LDEPTAFLDPAASGRVLAIIDKLHAEGVGIIHITHNMAEASRAQKIVVMDQGQICMQG
                     DPEEIFSRFEILQKLGLQLPQITELLIRLKQTGFPVRTGIVHMEDALKEIISMTARRK
                     D"
     gene            1365..2162
                     /locus_tag="PLF77_10690"
     CDS             1365..2162
                     /locus_tag="PLF77_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HPC09364.1"
                     /translation="MYRTGSFIRGESFIYRLDPRLKLAVTVLLSLLIFQVEQPLVFAS
                     GIGLFLLAMASGIRLRTIGESIRPVLFFIVLIFLSHLLFSDDKRSMMTIPFLGIAFSS
                     AGLKQGTLVVGQFLCLIVGAVLLTMTTAPSQIIAALRYFLKPFKKLRLPVDDVAVMVA
                     LALRLMPLLLMEKERIDAARKARGFCLRRQSVAGRMNSFLSLISKMLLGIFRKADDIA
                     LAMEARSYRRGGRSSFVELKFSSADYVVLVFLAIFLVIFVALNSHFG"
     gene            2191..3018
                     /locus_tag="PLF77_10695"
     CDS             2191..3018
                     /locus_tag="PLF77_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HPC09365.1"
                     /translation="MVDTQNKLKRAVADFERAKILSIDTEYDSFHYFREKLCLIQIHA
                     NHTTYLFDPLGDVALSFLGDFFDNQRIVKILHAADNDIRLLRRDYGFGFRNIFDTHRA
                     AFLLGFRELSLDKMIKEFVGVELKKNKKIQRSHWDNRPLTEEQVEYAVQDVIYLPDLY
                     QKQTAELTAKNLQESAREAFARIAETDWREKKFNRWGYAKIKGYNALNQEQKDLLKKL
                     YLWRFHRAKEENRAVFMFLPDKNLLDLALDADHPEKYLSQRKMRIYGSEIETIIKRM"
     gene            complement(3191..3979)
                     /locus_tag="PLF77_10700"
     CDS             complement(3191..3979)
                     /locus_tag="PLF77_10700"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09366.1"
                     /translation="MKKLAMTQEKTASLMMLVMFLMIVFIGACATTPATRAVWSKPNA
                     AQQDFAKDMDDCMKKAQATGSASTNVDMYNRCMETRGWTLKEVKSVSESSGITERYTF
                     ADGTRYEGTMANGKPHGRGTYNWTNGNKYEGDWFEGKMSGKGKYTCKNGREFTGNFEN
                     NKPVGFTINCSGNVYYVPDSRQKAQPRHGSYTDANGNKYEGQIINGKLHGKGKITSPS
                     GNKYEGDFVNGKIEGRGTYTCSTGKTFTGYFKNNKPVGFKVKCK"
BASE COUNT          912 a          944 c         1078 g         1144 t
ORIGIN      
        1 caaccagagg tctggtctat gcggcgcttt tcggagccat gacggcagcg ggggcgttga
       61 ttgttattcc tcttccgccc gtgccggtta ctgcgcagac attttttatg aatgtcgccg
      121 cccttcttct tggcggtccg ttggcggttg tcagccagat aatttatgtc ttgctgggcg
      181 tggtgggaat gccggttttt gccgggggca aagccgggct gggcattttg ttcgggccaa
      241 ccggcggtta tctattcggt tttatcattg ccgcttttgt catcggccat gtccatcgca
      301 tgaaaaaaaa cgccggcgcg gtctggcagg tattttccat ggtgctgggc atgctggtca
      361 tttatgttgc gggaagcatt caattggcat ggattgcgaa catgagtctt ccgcaggtgc
      421 tgatcgtcgg ggtagtacct tttatcccgg gcgatcttat caaaatagcg ctggctgtcc
      481 tgatcagttc ccggttaaaa aaccgcatag agatttgagg aaacgacgtt gattgttctg
      541 gaagatgttt attgccggta tgaaccggaa ggcattgaag ccctacgcgg cgtttctctg
      601 caaatccggg agggagaacg tctggcttta atcggtccga acggctgcgg caaaaccaca
      661 ttgatcaggc attgcaatgc gctgcttgtg cccgaaaaag gtcatgtcac cgtcggcgga
      721 ttaaggacgg atcataaaga gaaccaaccc gatatccggt cacgggtggg catggttttc
      781 cagaatcccg atcatcaaat cgtcgggatg acggtcgaag acgatgtggc attcggtccg
      841 gaaaatttgc gtctgccagc ggcgaagatc agggcaagga ttgccgaatc cctcagccgg
      901 gtgggcattt acggacttgc cgcccgcaat atcgaagatc tttccggcgg cgaaaaaaga
      961 ctggtggcga ttgccggcgt gctggctatg cggccgcgtt atattgctct ggatgagccg
     1021 accgcctttc tggatccggc agcctccgga agggtgctgg cgattatcga caaactgcat
     1081 gccgaaggcg tgggaatcat tcacatcacc cataacatgg cggaagcttc ccgcgcgcag
     1141 aaaatcgtcg tgatggatca gggccaaatc tgcatgcagg gcgatccgga ggaaattttc
     1201 agccggtttg aaatcctgca aaaactgggg cttcagcttc cccagattac ggaacttctc
     1261 atccggctga agcaaacggg ctttccggtc cgaacgggca ttgtccatat ggaagacgcc
     1321 ttaaaggaaa ttatttccat gaccgcgcgt cgtaaggatt gaacatgtac cgcaccggtt
     1381 cgtttatcag gggagagtcg ttcatttacc ggctggatcc ccgcctgaaa ctcgctgtaa
     1441 ccgtcttgtt gagtctgttg atttttcagg tcgaacaacc gctggttttt gcttcaggca
     1501 tcggtttgtt tttgctggct atggccagcg gcatacgtct gcggacaatc ggtgagtcga
     1561 tcagaccggt ccttttcttt atcgtcctga tttttctgtc gcatctttta ttcagcgacg
     1621 ataaacgctc catgatgacg atcccttttt tggggattgc cttttcgtct gccggattaa
     1681 aacagggaac ccttgtggtc ggacaatttt tatgtctcat tgtcggggcc gttttgttga
     1741 cgatgaccac ggcaccttcc cagatcattg cggcactgag atatttttta aaacctttca
     1801 aaaaactgcg gctgccggtg gatgacgttg ccgtgatggt cgcgctggct ttaaggctca
     1861 tgcccctgtt gctgatggaa aaagaacgaa tcgatgccgc ccggaaagcc agagggtttt
     1921 gtctgcggcg acagagtgtt gccggccgga tgaattcctt tttgtctttg atttcaaaaa
     1981 tgctgctggg cattttccgg aaagcggacg atatcgccct ggccatggaa gcccgaagct
     2041 accggcgtgg cgggcgttcg tcctttgtgg aattgaaatt ttcgtccgcc gattatgttg
     2101 tccttgtttt tttggcaatt tttctggtta tttttgtggc attaaattcg catttcggtt
     2161 aaattacccg ccatgaatgc gaaatggttt ttggtcgata cacaaaacaa actgaagcgc
     2221 gccgttgctg atttcgagcg ggcaaaaatt ctcagtatcg atacggaata cgattcgttt
     2281 cattatttca gggaaaaact ctgtctgatt cagatccatg ccaatcacac gacctacctc
     2341 tttgatccgt tgggcgatgt ggctttgtct tttttaggcg atttttttga taatcagcgt
     2401 attgtcaaga ttcttcatgc tgcggataac gatatccgtc ttctccggag agattacgga
     2461 ttcggtttca gaaatatttt tgatacacac cgggcggcgt ttcttctggg tttccgggag
     2521 ctgtctctgg acaaaatgat taaagaattt gtcggcgtgg aattgaaaaa aaacaaaaaa
     2581 atccagcgtt cccattggga taatcgtccg ctgacggagg aacaggttga atacgccgtc
     2641 caggatgtga tttaccttcc cgatctttat caaaagcaga cggcggaact gaccgccaaa
     2701 aatcttcagg aatccgcgcg ggaagcgttt gccagaattg ccgagacgga ctggcgggag
     2761 aaaaaattca accggtgggg gtatgcaaaa ataaaaggtt ataatgccct caatcaggag
     2821 cagaaggatc tgctgaagaa gttgtaccta tggcgttttc accgggcgaa ggaagaaaat
     2881 cgcgcggtct tcatgtttct gccggataaa aatcttttgg atctcgcgct ggatgcggat
     2941 catcccgaaa aatatttatc acaaaggaag atgcgaattt acggttcgga aatcgaaacg
     3001 attataaaaa ggatgtaatc atttgaaatt cgatcctgag gatgtttcca gcggcaaaaa
     3061 agccttcaaa tgccgatctg tctttcgtag aggtggcccg aaaataaccg gattatcaag
     3121 ttgttgtcga aagagatttg ataacggtct ttcgtgtttt caaagcgggg ggggggaagc
     3181 gtgacgggat tcatttgcat ttaactttaa agcccaccgg tttattgttt ttaaaatagc
     3241 ctgtaaacgt cttgccggtg ctgcaggtat aggttccccg gccttcgatt ttgccgttta
     3301 caaagtcgcc ttcatatttg ttgccgctcg gagaggtaat cttgcccttg ccgtgtaatt
     3361 tgccgttgat aatctgcccc tcgtatttat tgccgttggc atcggtatac gacccatgtc
     3421 ttggctgggc cttttgacgg ctgtccggaa cgtaatacac attgccgctg cagttgatcg
     3481 taaaacccac cggtttgtta ttttcaaaat tgccggtaaa ctcgcggccg tttttgcagg
     3541 tgtatttccc cttgccgctc atttttcctt caaaccagtc gccctcgtat ttgttgccgt
     3601 ttgtccagtt gtaagttccc cggccatgcg gtttgccgtt tgccattgtt ccttcataac
     3661 gggtaccgtc ggcaaaggtg taacgctctg taataccgga actttcagaa accgatttga
     3721 cttctttcaa tgtccagccc cgtgtttcca tgcagcggtt atacatatcc acattggtcg
     3781 acgcgctacc cgtcgcctgg gcttttttca tacagtcatc catgtctttt gcaaaatcct
     3841 gctgtgcggc attcggcttt gaccagactg ccctggtggc cggcgtcgtg gcgcatgctc
     3901 cgatgaaaac gatcatcaga aacatgacga gcatcatcag cgatgccgtt ttttcttgag
     3961 tcatggcaag ctttttcatg tttcccccga agattgggtt tgctgaagaa ttaacattgt
     4021 tcagcaagac gatgataact gctgaatgag attctggtgg agatgagggg gatcgaac
//
LOCUS       DAOOLB010000138         4060 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_26625_length_4060_cov_45.949064, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000138 DAOOLB010000000
VERSION     DAOOLB010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26625_length_4060_cov_45.949064"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="PLF77_10705"
     CDS             complement(<1..602)
                     /locus_tag="PLF77_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09367.1"
                     /translation="MKMNLKQTRVRPLFFKIFCALFAVTLFVAFSSSTGYAPTRMSDS
                     ELSEIEGQAFFKIEHFPASSEFWDVVDPSGAPVGRKYNTGSQNVIRISMGIDVEAHAF
                     IESQKMGYYNGGWDQDHTNFWVGDRSSTGDGGPLRLNGLYIDLGYDNIGNAANRTLNY
                     VEMGSMHVTGNVTQTINSINGLVASGTGSNNGVMLRQTASG"
     gene            complement(804..1670)
                     /locus_tag="PLF77_10710"
     CDS             complement(804..1670)
                     /locus_tag="PLF77_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09368.1"
                     /translation="MRTKLLFPLLVFVLLFCAMPVDAKTIVKIGRDVDISEDQQATGV
                     IAVGGQITVRGLVEKNVVAVGGSVVITGDAVVRGNVLCIGGVVVRGSGARIFGNVTEV
                     NSANFLAAVSSAFYEEEEEWSWLTDIIYFCFFTFLLMLALMLTFLFPRPLNAIIDSVA
                     AQKTKSFFWGILGALMIAPLFMLLALSFIGIPLIPLAFSLVLLAFIFGFLAMSALLGR
                     FVLTNFFRHHKLSLMKEALLGMMLWWVIGWAPFYTGMMIKAAVITMGFGGVLVALIDR
                     GRNRQKTARQAF"
     gene            complement(1683..2336)
                     /locus_tag="PLF77_10715"
     CDS             complement(1683..2336)
                     /locus_tag="PLF77_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HPC09369.1"
                     /translation="MDVLFHLIDFFLHLDKYLPLIIQSFGIWAYVIMFLVIFCETGLV
                     VTPILPGDSLLFALGAFAAQGALNIEILLISLCIAAVAGDTVNYSIGKFLGPKVFHYP
                     DSRFFKREYLVRTHQFYEKHGGKTIIIARFIPIIRTFAPFVAGIGTMKYLRFISYNVV
                     GGISWICLFLLAGYYFGGIPAVKRNFTLVIIAIIIISVMPGVIEYVRQKYFVSRAKN"
     gene            complement(2487..3980)
                     /locus_tag="PLF77_10720"
     CDS             complement(2487..3980)
                     /locus_tag="PLF77_10720"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPC09370.1"
                     /translation="MRLACVFNPFQYKLHEENLRVVQKYFGLFPPLSLAWVCAIAEKA
                     GHDALLIDARTLRLTREDVLDRLKQWKPDILGFMMTTYMFRETLEWVTFLKKHLKLPV
                     IIGGYNLRVYPNESLSVEGPDFGCFNSAFYTLPRLLEELEGRRRFADVPGLIYKENGR
                     TTMTPYGRDPVFDDYPNPARHLLPNDLYAEFTTERKNFTVMVTSKGCPNGCLFCEAGH
                     TSYNPRSPQTVLGEIKECRTRYGIREIDFFDYDFCIDRRRTQAICDGIIQSGMDITWA
                     CRTRVDCVDEALLRKMKAAGCSRIYFGIESGVQEILDRLNKGISLQQIRDTIETTRSL
                     GIRALGFFLVGSPGETRETFQQTVKFAKSLKLDYVQFSKLTAKPLTPYWRQAVDQTGV
                     DYWREYILGNIEDKSLARPWTALANDDIDRLAKKAYVTYHLRPMFLLRHTLQVKSFGE
                     FKRKFYAFLEMMLRQEPIGTLDENFVAYPEDMGKLCFYNQNFKKNKP"
BASE COUNT         1116 a         1036 c          970 g          938 t
ORIGIN      
        1 ccggaagccg tctgcctgag cataaccccg ttatttgatc cggtgccgga tgcaaccagt
       61 ccgttaatag agttaatggt ttgtgtaaca ttgcccgtaa catgcatact gcccatttca
      121 acgtaattga gtgtccggtt cgcagcattg ccgatattat catagcccag atcgatgtac
      181 aggccgttga gcctcaaagg cccgccgtca ccggtggaac ttcgatctcc cacccagaaa
      241 ttcgtgtgat cctggtccca tccgccgtta tagtaaccca ttttctgaga ctcgataaac
      301 gcatgggcct ccacgtcgat tcccatggag atgcgaatga cattttggct gccggtatta
      361 tattttctcc caacgggagc acccgacgga tcgacgacat cccagaattc actggatgcg
      421 ggaaaatgct ctattttgaa aaatgcctgg ccttcaattt cagaaagctc ggagtcggac
      481 atccgggtcg gcgcataacc tgtggatgaa gaaaaggcga caaataacgt taccgcaaac
      541 aaagcacaaa atattttgaa aaaaaggggc ctcactcttg tctgcttcaa gttcattttc
      601 atcccgaaac gggctcctta agaaatcatc atcagtttta tgttttaagg actaatattt
      661 ttacgagtga tttaccatat acagaccaaa aataaatgtc aagcgcaaat ttttccggtt
      721 tatttcttaa tcatcttatt gctttaacac ctttaaaaaa cagagccgtc tttcaaacag
      781 gcccggatgt cttttatggg aggctaaaaa gcctgacgag ccgttttttg ccggttgcgg
      841 ccccggtcga tgagggcaac cagcacgccg ccgaaaccca tggtgataac ggcagccttg
      901 atcatcatcc ccgtataaaa aggcgcccag ccgataaccc accataacat catgcccagc
      961 aaggcttctt tcatcaatga cagcttatga tgacgaaaaa aatttgtcaa gacaaaccgg
     1021 cccagaaggg cgctcatcgc caggaatcca aaaataaacg ccagaagcac aagagaaaaa
     1081 gcgagcggaa tcaaaggaat gccgataaag gaaagcgcaa gcagcatgaa aagaggagca
     1141 atcatcaggg cgcccagaat tccccagaag aaagattttg ttttctgagc ggcaacggag
     1201 tcaataatcg cattcaacgg acggggaaac aaaaacgtca gcatcagcgc cagcatcagg
     1261 agaaaagtga aaaaacaaaa ataaatgatg tccgtcagcc aggaccattc ctcctcttct
     1321 tcgtaaaagg cggaagaaac cgcggccaga aaattcgccg agttcacttc cgtaacgttt
     1381 ccgaaaatac gggccccgct gccccggaca acaacaccgc cgatacagag cacgtttccc
     1441 cgcacaacgg cgtcaccggt gatcacaacg gagccgccca cggcgacgac atttttttca
     1501 accagccccc ggacggtaat ctgaccgccg acggcgatga cgcccgtcgc ctgctgatct
     1561 tcggaaatgt cgacgtccct gccgattttg acaatggttt tggcgtcaac cggcattgca
     1621 caaaacaaca atacgaaaac gaggaggggg aaaagcaact ttgttctcat gttgcaccgt
     1681 tcttaatttt ttgcacgcga tacaaaatat ttctgccgga catattcaat gacacccggc
     1741 atcacggaaa tgatgataat cgcgatgatc accagggtaa aattcctttt gacggcggga
     1801 atgccgccga agtaatatcc cgccagcaga aaaaggcaaa tccaggaaat gcccccgaca
     1861 acattatagc tgatgaatcg cagatatttc attgtgccga taccggccac gaaaggggcg
     1921 aaggtgcgga taatgggaat gaaccgggcg atgataatgg ttttgccgcc gtgcttttcg
     1981 taaaactgat gggttcgcac aaggtattcc cgtttgaaaa agcggctgtc cggataatga
     2041 aacaccttgg gaccgagaaa tttcccgatg gaatagttga ccgtatcgcc cgcaacggcg
     2101 gcaatgcaga gcgatatcag taaaatttca atattcaacg cgccctgcgc ggcaaaagcg
     2161 cccagggcga acaaaagcga atcgccgggt agaatgggtg tgaccaccag gccggtctcg
     2221 caaaaaataa cgagaaacat gatgacgtag gcccaaatgc cgaaactctg gataatcagc
     2281 ggcaggtatt tgtccagatg aaggaaaaaa tctataaggt ggaagagcac gtccataagt
     2341 aatgtacgat gaaaacctca aaatataaat aaaggtgacg ttaccgtata gagaagaacc
     2401 tccggcctgt caacctgaaa aagatttttt ctgttcaaca atgccttctt cggcgatgca
     2461 tttttaattg ccggaattac acggggctaa ggtttgttct ttttgaaatt ttgattgtaa
     2521 aagcagagtt tgcccatgtc ttccgggtag gcaacaaaat tttcatccag cgtgccgata
     2581 ggttcctgcc tcaacatcat ttccagaaaa gcatagaatt tgcgtttgaa ttcgccaaag
     2641 cttttgacct gaagcgtgtg acgcaaaaga aacatcggtc gcaaatgata cgtcacataa
     2701 gccttctttg ccagacggtc aatgtcgtca ttcgccaggg cggtccaggg tcttgcaaga
     2761 gacttatcct cgatattacc gagaatatac tcccgccagt aatcgacccc cgtctgatca
     2821 acggcttgcc gccagtaggg cgtgagcggt ttggcggtga gtttggaaaa ttgtacataa
     2881 tcgagcttca gggatttggc gaattttacc gtttgttgaa acgtctcccg tgtttcaccg
     2941 ggcgatccga ccaggaaaaa tcccagtgcc ctgatgccca gacttctcgt ggtctcgatg
     3001 gtgtcgcgaa tctgttgaag gctgatccct ttgttcagac gatccaggat ttcctgcaca
     3061 cccgactcga tgccgaagta tatgcgggag catcccgccg ccttcatctt ccgcagcagg
     3121 gcttcatcaa cacaatcaac ccgggtgcgg cacgcccagg taatatccat gccgctttgg
     3181 atgatcccgt cacaaatggc ctgagttcgt ctgcggtcga tgcagaaatc ataatcaaaa
     3241 aaatcgatct cacgaatgcc gtaccgggtg cggcattcct ttatctcgcc caggacggtc
     3301 tggggactgc gcggattgta cgacgtgtgg ccggcttcgc aaaacaaaca gccgttggga
     3361 cagcctttgc tcgtcaccat gacggtgaag tttttgcgct ctgttgtaaa ttcggcgtaa
     3421 aggtcattgg gcaaaagatg gcgcgcagga ttcgggtagt cgtcgaaaac cggatccctg
     3481 ccgtacgggg tcatcgttgt ccgaccgttt tctttgtaaa tcagaccggg cacatcggcg
     3541 aaccggcgcc ggccttccag ctcctccagc aaccgcggca gggtgtaaaa agcgctgttg
     3601 aagcagccga aatcgggtcc ctctaccgac agggactcgt tcgggtaaac cctcagatta
     3661 taaccgccga tgattaccgg aagcttaaga tgttttttga gaaacgtcac ccattccaac
     3721 gtttcacgga acatataggt cgtcatcatg aaacccagga tgtccggttt ccactgtttc
     3781 aaccggtcca ggacgtcttc cctggtcaaa cgcagcgtgc gggcgtcgat cagcagcgcg
     3841 tcgtgtccgg ctttctcggc gatcgcgcaa acccaggcca gagagagagg cgggaaaagc
     3901 ccgaaatatt tttgaaccac tctcaggttt tcttcatgca gcttatattg aaatggattg
     3961 aaaacgcacg ccagtctcat ttttcctcta cacttttttc atgaataaca tgtactatat
     4021 cattcaacaa ggcattcccg acatattttc tttccgcgtg
//
LOCUS       DAOOLB010000139         3997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_27194_length_3997_cov_52.466261, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000139 DAOOLB010000000
VERSION     DAOOLB010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27194_length_3997_cov_52.466261"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(145..1968)
                     /locus_tag="PLF77_10725"
     CDS             complement(145..1968)
                     /locus_tag="PLF77_10725"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HPC09371.1"
                     /translation="MKVLVINSGSSSLKYEVFDLNAEQSLMSGAVSRIGTKEADHACA
                     CGDKKQLTKIEAPDHRAALDFVLQAITGMEGTPIKNLEEIDAVAHRIGHGGSLYRGPV
                     VIDQSVIDEIRRLIPLMPLHHPAMLAGIDACQKALPDIPHVAVFDTSFHSAIPDEAAI
                     YGLPHEFFTAGVRKFGFHGNSHEYVASKAAEYLETPMRRLNLISCHLGNGASVCAIQR
                     GHSIDTSMGFSPLEGLIMGTRIGDLDPGIIPYLMRQHHLDIDALDKMLNNKSGLLGIS
                     GVSSDMREIIAAADADNARALLAIKAFCYRIKKYIGAYHGVLGGADALIFTGGIGENN
                     RSIRSRCVQGLEKLGFAIDKVRNDRCEVHAEKPVCDISARYANTQILVIATDEELMIA
                     RQCARALDYTSSIKHHLLATDRRPIRVSVSVRHAHLSKSDVEALFGPGYVLTSKSPLY
                     IDSEFAANETVDLVGPRGRVNNVRIIGPERLKTQVEISRTEEFQLGIDAPVRESGDLD
                     GTPGIILEGPAGRIEIPEGVICAMRHIHMTPEDAELYGVKDKDIVMVRIEGDRELIFG
                     DVLVRVKPNYKLEMHIDTDEANAAELPPVSEGYLVRIESRS"
     gene            2267..3091
                     /locus_tag="PLF77_10730"
     CDS             2267..3091
                     /locus_tag="PLF77_10730"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPC09372.1"
                     /translation="MLPDIKTVELFRIGVLHISPFGVLAATGILTGRALALHRAKQAG
                     IPEEDMDDALLYTLLSAFFFAHIAEAVFYHPDQIMRQGPVYLLKFWKGLSSFGGFFGG
                     LFGFMFFVYRRKIRSPLLFAECILQGLVLGWIFGRLGCTIIFDHPGQLSDFFLAFQQP
                     GGARHNLGFYEFLLTLLVLFPVTLVLHRLQARPGSYTAAVLILYTPVRFFLDFLRLDA
                     AQNGDIRYGALTPGQYGSMILFVLGVAIAYRLMTSLPLPSGIVQPVKKKKKKNKER"
     gene            complement(3148..>3997)
                     /locus_tag="PLF77_10735"
     CDS             complement(3148..>3997)
                     /locus_tag="PLF77_10735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPC09373.1"
                     /translation="HVGRTMMSGFSNKLAFMTGGSSGIGLATANLLAAQGCNLVLFAR
                     GQKMLDEACKTVKTKMKSSSQKVNAVSMDVADNDDVQQKIKLAVEKFGVPDILINSAG
                     VGSGDYFENISYEQFDRLLKINVYGTRNTVSAILPFMKQKGGGHIVNIASVAGLIGMY
                     GYSLYCTSKYAIVGFSECLRSELKRFNINVTLICPPEVKTPFIEEEAKTLPPEARIVK
                     NLAGLLTPEQAAKAIVRGIQGRKFLFVPGLMARSLLFWHRISNGWLTRTPSDLIIKLA
                     ARRSRQ"
BASE COUNT          880 a         1050 c         1060 g         1007 t
ORIGIN      
        1 ctacgtttct gaaatttggg tctcattaaa aaagctctct tttcagggag agcttaaaat
       61 atttatcaca aattgaacac tctggcaaac agggaatcgc gctgccgtcc aatacgcctg
      121 tccttttttg cagaacagtc ccgtttaact gcgcgactcg atgcgcacca gataaccttc
      181 ggataccggc ggcagttccg ccgcgttggc ctcgtccgta tcaatatgca tttccagttt
      241 ataattgggt ttgacgcgta ccagcacatc gccgaaaata agttcgcggt cgccttcgat
      301 gcgcaccatg acgatatctt tgtccttgac gccgtatagt tcggcgtctt ccggtgtcat
      361 gtggatgtgc cgcatcgcgc agatgacgcc ttcgggaatt tcgatccgtc ccgccggtcc
      421 ctccagaatg atgccgggcg tgccgtccag atcgcccgac tcacgcacgg gagcatcgat
      481 gccgagctga aattcctccg tacgggatat ttccacctgc gttttgagtc gttccggccc
      541 gatgatgcgg acgttgttga cccggccgcg gggcccgacg agatcgacgg tttcgttggc
      601 ggcaaattcg ctgtcaatgt agaggggcga tttggatgtc agaacatatc ccggtccgaa
      661 cagcgcttcc acatccgatt tgcttaaatg cgcgtggcgc acggagaccg aaacgcggat
      721 gggacgccgg tccgtggcca gcaggtgatg tttgatggaa cttgtataat ccagcgcgcg
      781 ggcgcactgc ctggcgatca tcagttcctc gtccgtcgcg atgaccagaa tctgggtatt
      841 cgcgtagcgc gcgctgatat cgcagaccgg tttttccgca tggacttcgc aacgatcgtt
      901 gcggacttta tcgatggcaa atcccagttt ctccaggccc tgcacacagc gggaacggat
      961 gctgcggttg ttctcgccga taccgccggt gaaaatcaac gcatcggctc cgcccagaac
     1021 gccgtgatac gctccgatgt atttcttgat gcggtaacag aacgctttaa tggccagaag
     1081 cgcgcgggcg ttgtcggcat ctgctgcggc gataatttcc cgcatatcgg aactcacgcc
     1141 ggaaataccc agaagcccgc ttttgttgtt cagcattttg tccaatgcat cgatgtccag
     1201 atgatgctga cgcatcagat aggggatgat ccccggatca agatcgccga tgcgcgtgcc
     1261 catgatcagc ccctccagcg ggctgaagcc catggaggtg tcgatggaat gtccccgctg
     1321 aatagcgcac acgctggccc cgttgcccag gtggcaggaa atgagattca ggcgtctcat
     1381 cggagtctcc agatactcgg cagcctttga ggcgacatat tcatgcgagt ttccgtgaaa
     1441 gccgaatttc cttacgccgg cggtgaaaaa ttcatgaggc aggccgtaaa tggcggcttc
     1501 gtccggaatg gcgctgtgaa acgaggtgtc gaaaacggcc acatgcggga tatccggcag
     1561 cgctttttgg caggcatcga taccagccag catggcggga tgatgcagcg gcatcagggg
     1621 aataagccgc cggatttcgt caatcacgct ctgatcaatg accacggggc cgcggtacag
     1681 cgagccgccg tgaccgatgc ggtgcgccac ggcgtcgatt tcctccaggt tcttaatcgg
     1741 cgttccttcc atgccggtta ttgcctgaag gacaaagtcc aacgcggcgc gatgatccgg
     1801 cgcctcaatt ttcgtcaact gttttttgtc accgcaggcg cacgcatgat ctgcctcttt
     1861 cgtgccgatg cgcgagaccg cgccgctcat gagcgattgc tcggcgttga ggtcaaaaac
     1921 ctcatatttt aatgatgaac tgccgctgtt gatgacgaga actttcatgt tggatcctct
     1981 tgccggggtt gggttaagag tcctttgctc aaaagaccgg ggacgcggct tgccttgctt
     2041 cccgtatgaa aatgactttc agaaagtata ggaaaagcga aaaggcctgt caatgaaaaa
     2101 tccggacctt ccggttacgg atgtcccgaa cggtttgggg aataaaaaaa ataactgaac
     2161 tccgataaaa catgtgctac aaccagcagg cgatttcccg cttttttgaa aattcaaacc
     2221 cgggatggct gggattccct gatgaaaaga atggaggcat agacttttgc tgcccgatat
     2281 caaaaccgtc gaattattcc gtatcggtgt tttgcacatc agtcctttcg gtgtgctggc
     2341 cgccacaggc attctgaccg gtcgcgcgct ggcgttgcat cgggcgaagc aggcgggtat
     2401 tcccgaagaa gacatggatg atgccctgct ctatacgttg ctgtcggcat tcttttttgc
     2461 acacattgcc gaagccgttt tttatcatcc cgatcagatt atgcggcagg ggcctgttta
     2521 cctgctgaag ttctggaaag ggctcagttc tttcggaggc tttttcggcg gactcttcgg
     2581 gttcatgttt ttcgtttatc gaagaaaaat ccgctcgcct ctgctttttg ccgaatgcat
     2641 ccttcaggga ctggttctgg gatggatttt cggcagactc ggctgcacga ttatttttga
     2701 tcatcccggc caattaagcg attttttcct ggcctttcaa cagccgggcg gcgcacggca
     2761 taatctcggg ttttatgaat tcctgctgac cttgcttgtc cttttccccg tgacgcttgt
     2821 tctgcaccgc ctgcaggcca gaccgggaag ttataccgcc gcggttttga ttctctatac
     2881 gccggtgaga ttttttctgg attttctccg tctcgacgct gcacaaaacg gggatatacg
     2941 gtatggggcg ctcacgcccg gacaatatgg aagcatgatc ctgtttgtgc tgggagtggc
     3001 tattgcgtat cgactgatga cctctctgcc gctgccttcc ggcatcgttc aaccggtcaa
     3061 gaagaaaaaa aagaaaaata aagagcgata gaaagaaact ccacgtcaaa aaacatcgca
     3121 tcagacagaa tatttttgca gatatcttta ttggcgtgat cgtctggcgg cgagtttgat
     3181 aatcaaatca gaaggtgttc gcgtaagcca tccgttgctg atacggtgcc agaacagaag
     3241 agacctggcc atcaagccgg gtacgaagag aaacttcctt ccttggatgc cgcggacaat
     3301 tgcctttgcc gcctgttccg gtgtcagcag accggcaaga ttctttacga tccgcgcctc
     3361 gggcgggagt gtctttgctt cttcttcgat aaaaggagtc ttaacttccg gcggacaaat
     3421 gagcgtcacg ttaatattaa accgcttgag ttcgctgcgc aggcattccg aaaatcccac
     3481 gatggcatat ttacttgtac agtacagaga ataaccgtac atgccgatca atccggcaac
     3541 ggatgcaata ttaacgatgt gacccccgcc cttttgtttc atgaaaggca gaatggcgct
     3601 tacggtattc cgggtgccgt acacattgat tttgagtaac ctgtcgaatt gttcgtagga
     3661 aatattttca aaataatccc cggaaccgac accggcgctg ttgattaaaa tatcgggaac
     3721 gccgaatttt tcgacggcca gtttgatttt ctgctgaaca tcatcattgt ccgcgacgtc
     3781 catggagacg gcgttgacct tctgagaaga gcttttcatt tttgttttga ccgtcttgca
     3841 tgcttcatcc agcatcttct gccctcgggc aaataaaaca aggttgcatc cctgcgccgc
     3901 gagtaaattg gccgttgcca gaccgatgcc gctggaccct ccggtcataa aggcgagttt
     3961 attggaaaag ccgctcatca ttgtcctccc gacatgt
//
LOCUS       DAOOLB010000140         3861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_28507_length_3861_cov_42.713873, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000140 DAOOLB010000000
VERSION     DAOOLB010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28507_length_3861_cov_42.713873"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1268
                     /locus_tag="PLF77_10740"
     CDS             <1..1268
                     /locus_tag="PLF77_10740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09374.1"
                     /translation="ISSDLFSYIITDANGNYTLRLPAALVEQYNVILTPGKKGYTFTP
                     ARRTFDVGLTDITGVNFTAAEASVYGQADLAGTWRTNMLRTGTENGWVRARLTIGATG
                     VATCESYETSTSAGDTTCPADFDLTFTVNPDTGAIIQSGAYAAGDGGHMTMNSDMDFA
                     AGTATAGTNYQIMIAQKEGTTNYSNANLASHNFVVHGMVVGQTNEWWYGTGATDTALV
                     TTMSEEVGPTGAPSATINGVTLAVAVDGVVTLVDALGARASDFEGFLSVDKKTMVGTY
                     TDINGDYQLMVVQITDGQAVANLTGSSYNHLLGVGDDVFWAHHDINVSRFETSFLPDI
                     LALTSNLDVMLSYNWNLSLVYTPVQKLGLVSVKKLTIAPNGQAIVTNPETDAIVFHGQ
                     LAFDGTFMVGTETMELTEGTFYTLNVITH"
     gene            complement(1352..2287)
                     /gene="epmA"
                     /locus_tag="PLF77_10745"
     CDS             complement(1352..2287)
                     /gene="epmA"
                     /locus_tag="PLF77_10745"
                     /inference="COORDINATES: protein motif:HMM:NF012379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HPC09375.1"
                     /translation="MNDFFQDNIFHPARKSQALEMRAKLIQGIRHFFINSSFLEVDTP
                     VRIPSPAPEEHIEALRSEDWFLQTSPELCMKRLLAAGYPRIFQICKCFRAGERGDLHL
                     PEFTMLEWYVAGFDYRQLMAQCRNLIVAVAQNQGLTEIKRDNRTIALTPPWESITVED
                     AFLRYAPLTLDEALGQGTFDEILVDCIEPRLGIGKPTFLYDYPASLAALAKLKDNHRT
                     VSERFELYIGGLEIANGFSELTDAAEQRKRFEEALKKRAEKKWSEYLMPEKFLQSLGH
                     LPPCAGIALGIDRLVMIITGANCIDDVVAFIPENL"
     gene            complement(2418..2981)
                     /gene="efp"
                     /locus_tag="PLF77_10750"
     CDS             complement(2418..2981)
                     /gene="efp"
                     /locus_tag="PLF77_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPC09376.1"
                     /translation="MYTASDLRKGLRLKIDGEPYVIVEFNFVKPGKGQALYRTKLKNM
                     INGNQFERTFRSVDTFEQADLREKTMQYLYKEGDKFCFMDNETFEQVYLTEEQVDDAV
                     NFMTDNIEVEILLFEDRPIGISLPNFVELVVTEAEPWAKGDTAAGSTKPVKVQTGYTI
                     QVPTFVTEGEKIRIDTRTGEYLTRVKG"
     gene            complement(3037..>3861)
                     /locus_tag="PLF77_10755"
     CDS             complement(3037..>3861)
                     /locus_tag="PLF77_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPC09377.1"
                     /translation="RAAEDPLQEDLHMPVPKLVHRYPDRCLAIVTETCAVYCRHCNRK
                     RFWSRPASLNLTDRLQKITRYIAESPRIREVIISGGDPLTLDDRVLEKILASLKAIPH
                     VDVLRIGSRVPVVLPMRITRTLCRMLKRYRPLWFNTQFNHPGEITPEAARACNMLQEA
                     GIPVSNQSVLLKGVNDSPDIMGDLLYGLQKMSVRPYYLFHCDPAQGCLHFRTEVPAGL
                     DMMEKIWRHCSGLCVPQYVLDVPGKVGKIPLNILSETMKNDLKKHQHFFDKRQEID"
BASE COUNT          932 a          964 c          997 g          968 t
ORIGIN      
        1 cgatcagcag tgatttgttc agctatatta tcacggatgc caacggcaat tacacattga
       61 ggctgcccgc agcgctcgtc gaacagtata acgttattct tacacccggt aaaaagggtt
      121 acaccttcac ccccgccaga agaactttcg atgtaggttt gaccgatata acaggcgtga
      181 acttcacggc tgcggaagca tccgtatacg gtcaggccga cctggccgga acgtggcgta
      241 ctaatatgct tcgaacgggc accgaaaatg ggtgggtgcg tgcccgcttg accatcggcg
      301 ccaccggtgt tgccacgtgt gagtcgtatg aaaccagcac gtcagcgggc gatacaacct
      361 gtccggccga ttttgattta acatttaccg tcaatccaga tacgggtgcg atcattcaaa
      421 gcggtgccta tgctgcggga gacggcggtc acatgaccat gaactccgat atggattttg
      481 ctgccggcac ggcaaccgca ggaacaaact accagatcat gatcgcgcag aaagaaggga
      541 ccaccaatta cagcaatgcc aatctggcaa gtcataactt cgtcgttcat ggcatggttg
      601 tcggtcaaac caatgagtgg tggtatggaa caggagcaac cgataccgct ctggtaacaa
      661 cgatgagcga agaagtaggt cccacaggtg ctccttctgc aaccatcaat ggcgtaacgt
      721 tagccgtagc ggttgacggt gttgtgaccc tggtggatgc tttaggggca agagccagtg
      781 atttcgaagg atttctctcc gtcgataaaa agacaatggt tggaacctat accgatatta
      841 atggcgatta tcagttaatg gtcgttcaga ttaccgatgg acaggctgtg gccaatttga
      901 ccggatcgtc ctataaccat ctgctgggtg tcggcgatga cgtcttctgg gcgcatcatg
      961 atattaatgt aagccggttt gagacgtcct tcctgccgga tatattggcg cttacctcca
     1021 atttggatgt catgctttcc tacaactgga atttgtctct ggtttataca ccggtacaga
     1081 agttgggtct ggtcagtgtc aagaaactga ccattgctcc caacggtcag gcaatcgtca
     1141 cgaatcctga aaccgatgca attgttttcc atggacagct ggcatttgac ggtacgttca
     1201 tggttggtac ggaaacaatg gagttgacag aaggcacttt ctatacactg aatgtcatca
     1261 cccattaatc actaaaaacc agataaacaa aaacccgccc cttattcggg gggcgggttt
     1321 ttttatgcat taatttctta acaacgatgg atcaaagatt ttccggtata aacgcaacca
     1381 catcatcaat gcagtttgca ccggttatga tcatcaccag ccggtcaatg cccagcgcaa
     1441 tgccggcaca gggcggcagg tgtcccaggg actgaagaaa tttctccggc atcagatact
     1501 ccgaccattt tttttcagcc cgttttttca gcgcctcttc aaaacgtttt ctttgttccg
     1561 ccgcatcggt cagttctgaa aagccgttgg caatttccag cccgccgatg tatagctcga
     1621 acctttcgct gacggtgcga tgattgtctt taagtttcgc cagcgccgcc agtgaggccg
     1681 gatagtcata aagaaacgtc ggtttgccga ttcccagccg gggctcgata cagtcaacaa
     1741 ggatttcatc gaacgtgccc tgccccagag cctcatccag ggtaagcggc gcgtatctta
     1801 aaaaggcgtc ttccacggtg atgctctccc acggcggtgt taaggcaatg gtccggttgt
     1861 ctcttttgat ttcagtaagg ccctgatttt gtgcaaccgc aacgatcagg ttccggcatt
     1921 gggccatgag ttgccggtaa tcgaatccgg cgacatacca ctccagcatc gtaaactccg
     1981 gcagatgcag atcgcctctc tcgccggcgc ggaaacattt gcagatttga aaaatacgcg
     2041 ggtagcctgc ggccagcaac ctcttcatgc agagctccgg cgatgtctgc aaaaaccaat
     2101 cctccgaacg gagggcctcg atatgttctt ccggcgcggg cgaggggatc ctgaccggcg
     2161 tgtccacttc cagaaagctg ctgttgatga aaaaatgacg gatgccttga atgagtttgg
     2221 ctcgcatctc caaggcctga gacttgcgcg ccggatgaaa gatattatct tgaaaaaagt
     2281 cgttcatatt gattaagctt tttcaaattg tctcagaggc aaggcgcacg aaggatgcgg
     2341 agtgaagcgt attatgatac gacgcaagga cgcaggctga agcgcaacgc agcagataag
     2401 cattttgaaa agctgcgtta acctttgact ctagtcaggt actcacccgt tcttgtgtca
     2461 attctgattt tctcaccttc ggttacgaag gtcggaacct gaatcgtata gcctgtctgt
     2521 accttgaccg gtttggttga accggcggca gtgtcacctt tggcccaggg ttcggcttcg
     2581 gtgacgacca gttccacaaa gttgggcagg ctgatgccga tgggccggtc ttcaaaaaga
     2641 agaatttcca cttcgatgtt atccgtcata aaatttacgg catcatcgac ctgttcttcc
     2701 gtcagataaa cctgctcgaa ggtttcattg tccatgaagc agaatttgtc tccttcctta
     2761 tatagatact gcatggtctt ttcccgtaaa tccgcctgtt cgaatgtatc tacagaacga
     2821 aatgttcgtt caaactgatt gccgttgatc atgtttttga gtttggtgcg atacagggcc
     2881 tgtcctttac cgggcttgac gaaattgaat tcaacgatga cataaggttc gccatcgatt
     2941 ttcagccgca gtccttttct taaatcactg gcagtgtaca taaattttct ccttgcttag
     3001 tgtggcaaaa tcgcctgttt tgcctcaggc gcttctctaa tctatttctt gacgcttgtc
     3061 aaaaaaatgt tgatgtttct ttaaatcatt tttcatcgtt tcggacagga tgtttaaggg
     3121 gatttttccg accttgccgg ggacatccag aacatattgc ggcacgcaaa gtccggaaca
     3181 gtgccgccat attttctcca tcatatcaag gccggccggt acctcggtgc gaaaatgcag
     3241 acacccttgt gccgggtcgc aatggaaaag ataatagggg cggacggaca ttttttgcag
     3301 accgtaaaga agatcaccca tgatgtccgg ggaatcgttg acgcctttga gcagaaccga
     3361 ctggttggag acgggaatgc ccgcctcctg tagcatatta caggcccgcg ccgcctccgg
     3421 tgtaatttcc ccggggtgat tgaactgcgt attgaaccac aggggccggt atcgtttcag
     3481 catccggcag agcgtccggg tgatgcgcat cggcagaaca acaggcacgc ggctgccgat
     3541 acgcagaacg tcaacatgcg ggatggcttt cagagaagca aggatttttt cgagaacacg
     3601 gtcatccagg gtcaggggat cgccgccgga aataatcact tcccggatgc ggggcgattc
     3661 ggcaatgtaa cgggttatct tttgcagtcg gtcagtcagg ttcagggaag cggggcggga
     3721 ccagaaacgc tttcggttac agtgccggca gtacaccgcg caggtttccg tgacaatcgc
     3781 cagacaacgg tccggataac ggtgcaccag tttgggaacc ggcatatgca aatcttcctg
     3841 aaggggatct tccgccgccc g
//
LOCUS       DAOOLB010000141         3814 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_29002_length_3814_cov_45.658154, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000141 DAOOLB010000000
VERSION     DAOOLB010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29002_length_3814_cov_45.658154"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="PLF77_10760"
     CDS             complement(<1..1361)
                     /locus_tag="PLF77_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPC09378.1"
                     /translation="MSENPLHLLMNPQSIAVAGANNNPSKMGTIQALNIVKGGYGGRF
                     YPIHPVEKTVLGCPAYATPEVLPEAPDLAILIVPIRAVASLLEGFGKIGTKRAIVITA
                     GFKETGAAGRDMEKKINEIADRYQMRFVGPNCMGVINSGISLNTTVLATSREPGLLGF
                     ASQSGTFLSQTLPYLNKRGIRFSKAISLGNEANINMIDALEYLGEDEQTKAIILYIEG
                     IREGRRFLDVARNITPHKPIAALYVGGSASGARAGLSHTGAMAGPDFLYNGIFKQAGI
                     IRVNSIEDLYYHGWTLATQPPMRGKRVGVMTNSGGPSTTISYTCDAVGLEVPRFSDGL
                     QNEIRKHIEPHASASNPVDMTFDLSMNKLALTLPEMVMKSGEVDAVVLHGTMMTGYLK
                     EVYPTLKDIIGNISLEDFLKYGQMDRTIANETFKLPSKYNMPMLISSFFDHEDNYIKG
                     YQDT"
     gene            complement(1361..1963)
                     /locus_tag="PLF77_10765"
     CDS             complement(1361..1963)
                     /locus_tag="PLF77_10765"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPC09379.1"
                     /translation="MPPKVIYSRSDIIDSSLRIVKEKGFKQLSAREVARQLISSTRPV
                     YEHFQSMDELKKAVLQKTVDLLYDYVTRQYTKNAFLNTGVGYVLYARDHKEFFKMIFL
                     EDNDASGIIDEMLKKLDKEIVKVPELKKLAPAERKDLLRNGWIYTHGCAVMVFAGYIK
                     NNQDKQIIKMLAEAGPIFIEDALRKHNADALINTTIKEIE"
     assembly_gap    2036..2135
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2234..3715
                     /gene="lysS"
                     /locus_tag="PLF77_10770"
     CDS             2234..3715
                     /gene="lysS"
                     /locus_tag="PLF77_10770"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HPC09380.1"
                     /translation="MEDNELLNIRREKIADLKSAGIDLYPNDIKPKNTTQEITLKFGD
                     MDNDSLAKQEETFSVAGRLMAVRNFGKAAFIKIQDYKGQIQGYIGKNSLSPEEYFVFK
                     KLDIGDIIFISGKLFRTKTNELTIEAQKLMLLSKSIRPLPEKWHGLTDIETRYRQRYL
                     DLIANPKVKEIFHRRSLIIKLIRQFMDEHDFLEVETPMMQPRAGGAAARPFKTHHNAL
                     DMDFYLRIAPELYLKRLVTGGLERVYEINRNFRNEGISTFHNPEFTMMEFYQSYATYE
                     DLMAFTEDLFCFLAKNIFGGLKFNYQDTEIDLTPPWRRISVKDALLQLANMDAAALDD
                     PALALSFARKVGCKVNDNDPLGKILMAIFDEVVEKKLIQPTFVTHYPVVISPLSRRSK
                     NDPDLTDRFELYIYGREIANAFSELNDPVDQKGRFLQQLHEREAGDDEAHEMDEDYVR
                     ALEYAMPPTAGEGIGIDRLVMLFTDSSSIRDVILFPLLRTRQE"
BASE COUNT          926 a          922 c          903 g          963 t
ORIGIN      
        1 gtatcctgat agcccttgat atagttgtct tcatgatcaa agaaagaaga tatcagcatc
       61 ggcatgttat acttagatgg caatttaaaa gtttcgttgg caatcgtccg gtccatctgg
      121 ccgtatttca aaaaatcttc cagagaaata ttgccgataa tgtccttcaa cgtgggataa
      181 acctctttca gataaccggt catcatggtt ccgtgcagaa cgaccgcatc gacctcgccg
      241 cttttcatca ccatctccgg cagggtgagc gcgagtttgt tcatgctcag gtcaaacgtc
      301 atatcgacgg gattggacgc agaggcgtgc ggttcaatgt gttttctgat ttcgttttgc
      361 agcccgtcgg aaaagcgggg cacttccaga ccgaccgcat cgcaggtata ggaaatggtt
      421 gtggagggcc cgccggaatt ggtcatgacg ccgactcttt tgccgcgcat cggcggttgt
      481 gtggccagcg tccagccgtg atagtaaaga tcttcaatgg agttgacccg gatgatgccg
      541 gcctgcttga aaataccgtt atagaggaaa tccggaccgg ccatggcgcc ggtgtgactc
      601 aaccccgcgc gcgcgccgga ggcggaaccg cccacgtata acgctgcaat cggtttgtgc
      661 ggtgtgatgt ttcgggcaac gtcgagaaac cttcgccctt cgcggatgcc ttcaatgtaa
      721 agaatgatgg ctttggtttg ttcatcttct cccagatatt ccagcgcgtc aatcatattg
      781 atgttggcct cgttgcccag actgatggcc ttgctgaaac gaatccctct tttgttgaga
      841 tagggcagcg tctggctcag gaatgtgccg ctttgcgacg caaatcccag aaggcccggt
      901 tccctggacg tggctaaaac ggtggtgttt aatgaaatcc cggaattgat gacacccatg
      961 cagttgggac cgacaaaacg catctgatag cggtctgcta tttcgttgat ctttttttcc
     1021 atgtcccgtc ccgccgcgcc ggtttcctta aacccggcgg tgattacgat ggctctcttc
     1081 gtgccgattt tgccgaagcc ctccagcagg gaagcaaccg ctcgtatggg cacaatcagg
     1141 atggccagat cgggcgcttc gggcaaaacc tccggtgtgg cgtaggcggg gcaacccaaa
     1201 acggtttttt ccacgggatg aatcgggtaa aatctgccgc catagccgcc cttcacaata
     1261 ttcaacgcct gtatagttcc catcttcgac gggttgttat tcgctccggc aaccgcaatg
     1321 gattggggat tcatcagcaa atgcaatgga ttttccgaca ttattcaatc tcctttatcg
     1381 ttgtattgat gagcgcatca gcattgtgct ttcgaagtgc atcttcaata aaaatgggac
     1441 ctgcttcagc aagcatctta ataatttgtt tatcctgatt gttttttatg tatccggcaa
     1501 aaaccatgac ggcacatcca tgggtgtaga tccagccgtt tctcaacaga tcctttctct
     1561 ccgccggagc aagcttcttg agttccggca ccttcacgat ctctttatcc agctttttca
     1621 gcatttcgtc gatgattccg ctggcatcat tatcctccag gaatatcatt ttaaaaaatt
     1681 ctttatgatc ccgggcatac aaaacatagc cgacgccggt attcaggaag gcatttttcg
     1741 tgtactgccg cgtgacataa tcatagagaa gatcaacggt cttttgcagc accgcctttt
     1801 tcaactcgtc catggattga aaatgttcgt ataccggccg ggtggaggaa atcaattgcc
     1861 tggcaacttc gcgggcgctc agttgcttaa atcccttttc tttgacgatt ctcaacgagg
     1921 aatcgataat atcggacctg ctataaataa cttttggcgg catgcaattc ccggtctgtt
     1981 taaattacat gtgtaatgta acgtgtgtta tgtattatgt caagcgttta ttgatnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgac gtcgcatgga ggcctattcc
     2161 ccttattatc gttatacttg actatttggc taaaaaaata tagttaagca gactttacaa
     2221 aagtggagaa gctatggaag ataatgaact tttaaatatc cgccgtgaaa aaattgccga
     2281 tctgaaatcg gcgggcattg atttataccc caacgacatc aaaccaaaaa acacaacaca
     2341 ggaaataacg ttgaaattcg gcgatatgga taacgattcc ctggcaaaac aggaagagac
     2401 tttttccgtt gccggacgtc tgatggccgt gcgcaatttc ggcaaagcgg cttttatcaa
     2461 aattcaggat tacaagggac agatccaggg ctacatcggc aagaacagtt taagccccga
     2521 agaatatttt gttttcaaga aactcgacat cggcgacatc atctttatct cgggtaaatt
     2581 attccggaca aagaccaacg agctgaccat tgaagcgcaa aaactcatgc tgttatcgaa
     2641 atcgatacgt cccctgcccg aaaaatggca tggcctgacc gacatcgaaa cacgataccg
     2701 ccagcgatat cttgatctga tcgccaaccc caaggtgaag gaaatttttc accggcgcag
     2761 cctcattatc aagttgatcc gtcagttcat ggatgagcat gattttctgg aggtggaaac
     2821 cccgatgatg cagccgcgcg cgggaggcgc cgcggcccgg ccgtttaaaa cgcatcataa
     2881 cgctctggac atggattttt atctgcgcat tgcgccggaa ctttatttga aaagactggt
     2941 gaccggcggc ctggagcggg tctacgaaat caaccgcaat ttccgcaatg agggcatctc
     3001 caccttccac aatccggaat tcaccatgat ggagttttat cagtcctatg ccacgtatga
     3061 agatttgatg gcctttaccg aagacctgtt ctgttttctg gcgaagaaca tattcggcgg
     3121 tctgaaattc aattatcagg atacggaaat cgatctgacc cctccgtggc ggcgcatttc
     3181 cgtcaaagac gctctcctgc agctggcgaa catggacgcg gcggcgctgg acgatccggc
     3241 gctggcttta tcttttgccc gcaaggttgg ttgcaaggtg aacgacaatg atccgttggg
     3301 aaaaatctta atggctattt ttgacgaggt ggtggaaaag aaactgatcc agccgacatt
     3361 tgtcacccac tacccggtcg tcatttctcc gctctcccgg cgatccaaaa atgatcccga
     3421 tctcaccgac cggtttgaac tgtacattta cggtcgagag attgccaatg cgttttcgga
     3481 attgaatgat ccggtcgatc agaaaggacg atttctgcag cagctccatg agcgggaagc
     3541 cggcgacgac gaagcccacg aaatggacga ggactatgtc cgcgccctgg aatacgccat
     3601 gccgccgacc gccggagaag gcatcggcat cgaccgtctg gtgatgcttt ttaccgacag
     3661 ttcctccatc agagacgtta ttttattccc gctgttgaga accagacagg aataaacgga
     3721 ttattgacgg cgttgccgaa ccggacttat cccggaaaaa ggatataaca ataccgatgc
     3781 cctacgaact tttcatcagc aggcgttatt taaa
//
LOCUS       DAOOLB010000142         3790 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_29234_length_3790_cov_46.710843, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000142 DAOOLB010000000
VERSION     DAOOLB010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29234_length_3790_cov_46.710843"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLF77_10775"
     CDS             <1..294
                     /locus_tag="PLF77_10775"
                     /inference="COORDINATES: protein motif:HMM:NF017494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HPC09381.1"
                     /translation="PKLIENGLRGMIGKGNRSPEVVEAIKKYKSVYFGAIGGIAALMS
                     QCVKKVEMVAYEDLGPEAIRKLTVRDFPLVVVNDSSGKDLYVSAKQKWHISLI"
     gene            393..941
                     /locus_tag="PLF77_10780"
     CDS             393..941
                     /locus_tag="PLF77_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HPC09382.1"
                     /translation="MIEVRWHGRGGQGAVTSVELLAQAAIAAGKYAQGFPSFGPERRG
                     APVAAFTRVADKKINVRSGIYEPDVVLVLDSSLIGLVNVLDGLKPGGKLIVNTHKTPE
                     EIRKALDYKGILAIVDGTGIARKEMGVPIANTTMIGALLKVAGIMELDAMKESIEHRF
                     GRIAQKNLNAMKRAYDEITIYN"
     gene            955..1233
                     /locus_tag="PLF77_10785"
     CDS             955..1233
                     /locus_tag="PLF77_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPC09383.1"
                     /translation="MALKTWKELPIGCVVSEPGSASEYHTGSWRSQRPIWDNAKCIKC
                     GICYVYCPEGCVQQTEDGFFQADLDYCKGCGICAHECWPRAIKMLEEE"
     gene            1235..2410
                     /locus_tag="PLF77_10790"
     CDS             1235..2410
                     /locus_tag="PLF77_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HPC09384.1"
                     /translation="MGKRIGMEVAIAVAEAVGLCDIDMAAVYPITPQSHIAEHLSDLV
                     ANGEIDAEFVTVESEHSAMSAVIGASGAGARAFTATSSQGLMLMHELLPIASAMRLPI
                     CMAIANRAVSGPLNILNDHTDIMPQRDSGWISLFVENGQEAIDMSIIAFKIAEHKDVM
                     LPVNVNIDGFQLTHMVEPFEMPSKEEVDRFLPPFVPHATLHPAKPVTMGAFAMSDYFT
                     EIMKAKDEALKNSKKVIANVLDEWGKMFGRNYKLVETYKSEDAEVIMLTMGSMGETAQ
                     MAIDDLRAKGVKAGLVKLRLWRPFPFEEIKAAIKNAKKLIVTDRAISFGGPGGPVFSE
                     IKSAMYAATQRPLIYNYIYGLGGRDVTVGDFIGMFEKVLADTEHKAVDTYEFWGVRE"
     gene            2410..3360
                     /gene="porB"
                     /locus_tag="PLF77_10795"
     CDS             2410..3360
                     /gene="porB"
                     /locus_tag="PLF77_10795"
                     /EC_number="1.2.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019164 - pyruvate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate synthase subunit PorB"
                     /protein_id="HPC09385.1"
                     /translation="MNIVENFDLFAPRLINKKELISSGHRACSGCAEVLAVRLMCKAL
                     GENTVIASATGCMEIISSMFPTTAWRVPWIHVAFENTAAVASGIEAGLKALRRKGKID
                     DRYVKVVGMAGDGGTMDIGIQALSGAMERGHDMMFVCFDNEAYMNTGIQRSSGTPMGA
                     STTTAPAGKVSSGQKTWKKNMAEIMVAHEIPYVATVCPSYPLDFIRKIEKAKSIKGPS
                     YIHCFSMCPTGWRSPSHTAISMGRLAVETGVFPLFEVEHGHYRVSPDMPKKLRPIREY
                     FKPQGRFRHLKDNEIDQIQERVSMEYQKLMEKAKYLKTGN"
     gene            3399..>3790
                     /locus_tag="PLF77_10800"
     CDS             3399..>3790
                     /locus_tag="PLF77_10800"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPC09386.1"
                     /translation="MTKASSIAAKTKEIVKKHGKDKSALIAVLQDIQESFNYLPKDAL
                     KTAGKALGVPFSRVYEVATFYTAFSLKPRGEHIVKICMGTACHVRGAAAIQDKMERTL
                     CIKPGETTPDNKFSLETVNCVGACALGPV"
BASE COUNT          992 a          834 c         1054 g          910 t
ORIGIN      
        1 ccgaaattaa tcgaaaacgg ccttcgggga atgatcggca agggcaaccg ttcgccggaa
       61 gttgtcgaag ccatcaaaaa gtacaaatct gtttatttcg gagcgatcgg cgggattgcc
      121 gctttaatgt ctcaatgcgt caaaaaagtc gaaatggttg cctatgaaga cctcggtccc
      181 gaggcgatca gaaaactgac agtcagagat tttccgctgg ttgttgtcaa cgattcctcg
      241 ggcaaagacc tgtatgtttc ggcaaagcaa aaatggcata tctctttaat ataactagct
      301 atttcagcgt tcgtgaaaat ctgttgactg aaatttgctt ctatattatg aatgatggtc
      361 atttttgtat cacgataagg agacgaaaat aaatgataga ggttagatgg cacggacgtg
      421 gcggtcaggg agcggtgacc tcggtggagt tgctggctca ggcggcaatt gcagccggaa
      481 aatacgctca gggatttccc agtttcggtc cggaaagaag aggggcgcct gtagccgcat
      541 ttacccgtgt tgccgataaa aaaatcaatg tccgctcagg catctatgag cctgatgtgg
      601 tgctggtgct ggattccagt ttgatcggtc tggtgaatgt tctcgatggt ttgaaaccgg
      661 gcggcaaatt gattgtcaat acgcataaaa ctcctgaaga aattcgcaag gcgcttgatt
      721 acaaggggat attggccatt gttgacggca ccggaatagc acgtaaggaa atgggcgtgc
      781 ctattgccaa caccaccatg atcggcgctc ttttgaaagt ggccggcatt atggaactgg
      841 atgcgatgaa agaatctata gagcaccgtt tcggcagaat cgcgcagaag aatctcaacg
      901 cgatgaagcg ggcttacgac gaaataacaa tatacaatta aggggtagat ctcaatggca
      961 ttgaaaacat ggaaggaact tcccattggc tgtgttgtgt ccgaacccgg aagcgccagt
     1021 gaatatcata cgggaagctg gcggtctcaa agacccatct gggataatgc aaaatgcatc
     1081 aagtgcggca tttgttatgt ttattgtccg gaaggttgcg tgcagcagac cgaggacggc
     1141 ttctttcagg ccgatctgga ttattgcaaa ggttgcggta tttgtgcgca tgagtgttgg
     1201 ccccgcgcga taaagatgtt ggaggaggag taatatggga aaacgcattg gaatggaagt
     1261 ggcaattgcc gttgcggaag ccgtgggtct ttgcgatatt gacatggccg ccgtttatcc
     1321 tattacgccc cagtcgcaca tcgcggaaca tctttccgat ctggttgcca acggtgaaat
     1381 tgacgcggaa tttgtcaccg ttgaatccga gcattcggcg atgagcgcgg tgatcggcgc
     1441 ctccggcgca ggtgcccgtg cgtttacggc aacaagttct caggggttga tgctgatgca
     1501 cgaacttctt cccattgcgt cggcgatgcg cctgccgatt tgcatggcaa ttgccaaccg
     1561 cgcggtgtcc ggtcccctga acatattaaa tgatcacacc gatatcatgc cccagcgcga
     1621 ttcgggctgg atttctcttt ttgtggaaaa cggccaggaa gcgattgata tgtcgatcat
     1681 tgctttcaaa attgccgagc acaaggatgt catgctgccg gtcaatgtta atatcgacgg
     1741 tttccagttg acgcacatgg tggagccttt tgaaatgccc tcaaaggaag aagtggatag
     1801 atttctgccg ccgtttgtcc cgcacgcaac gctgcatccc gcaaaaccgg ttacgatggg
     1861 cgcttttgcc atgtcggatt attttacgga aattatgaag gccaaggatg aggcgctgaa
     1921 aaattcaaag aaagtcattg caaatgtatt ggacgaatgg ggcaaaatgt ttggccgcaa
     1981 ttacaaactg gtggaaacgt ataaatccga agatgccgaa gtcattatgc tgaccatggg
     2041 ctcgatgggt gaaaccgcac agatggctat tgatgacctt cgtgccaaag gcgtgaaggc
     2101 cggtctggtg aaattaagat tgtggcgtcc ctttccgttt gaggaaatca aagccgcgat
     2161 taaaaacgcg aaaaaactga ttgtgacgga tcgtgccata tcgtttggcg gccccggcgg
     2221 tccggttttt tcggaaatca aatccgccat gtatgcggca actcagcggc ctctgatata
     2281 caattatatt tacggactgg gtggtcgcga cgtgacggtg ggcgacttta tcggcatgtt
     2341 tgaaaaagtt ctggcggata cggaacataa agcagtggat acctatgaat tttggggagt
     2401 gagagaataa tgaatatcgt agaaaatttt gatttatttg ccccgcggct gattaataaa
     2461 aaagaactga tctcttccgg tcatcgcgcc tgttcgggtt gtgctgaagt tctggctgtg
     2521 agattgatgt gtaaggcgct tggtgaaaat acggttatcg cttcggcgac cggctgtatg
     2581 gaaatcatct ccagcatgtt tcccacaact gcctggcgcg tgccttggat tcatgtggct
     2641 tttgaaaaca cggcagccgt tgcatccggt atagaagccg gactcaaggc attgcgcaga
     2701 aaaggaaaga tcgacgaccg ttacgtcaag gtggtcggca tggcgggcga cggcgggacc
     2761 atggatatcg ggattcaggc cctttccgga gcgatggaac gcggtcatga tatgatgttc
     2821 gtctgtttcg acaatgaagc gtacatgaac acgggtattc agcgttccag cggtacgccg
     2881 atgggcgctt cgacgaccac ggcgccggcc ggcaaagtga gttccggaca gaaaacatgg
     2941 aagaagaaca tggcggaaat catggtcgcc cacgagatac cgtatgtcgc aaccgtttgt
     3001 ccgagttacc cgctggattt tatccgcaag attgaaaagg cgaaaagtat caagggaccg
     3061 tcctatatcc attgtttctc tatgtgtccg accggctggc ggtctccttc gcatacggca
     3121 atatccatgg ggcggctggc ggtggaaacc ggcgtatttc ctttgtttga agtggagcat
     3181 ggccattacc gggtttctcc ggatatgccc aaaaagctca ggcccatccg ggagtacttc
     3241 aagccgcagg ggagattccg tcatttgaaa gataatgaaa ttgatcagat tcaggaacgc
     3301 gtttccatgg aatatcaaaa actgatggaa aaagccaagt atctgaaaac aggcaattaa
     3361 gaggaatgtc ctcaaaagaa taccgaggtg aaaaaagcat gacaaaagct agcagcatcg
     3421 cagcaaaaac caaagagatt gttaaaaaac acggtaaaga taaaagtgca ctgattgccg
     3481 tcttgcagga tattcaggag tcctttaatt acctccccaa ggatgctctg aaaacggccg
     3541 gcaaagctct gggcgttccc ttcagccgcg tttacgaagt tgccacgttt tatacggcct
     3601 tcagtctgaa accgcgcggc gagcacatcg tgaaaatctg catggggacc gcctgtcatg
     3661 tgcgcggcgc ggcggccatt caggataaaa tggagcgaac attatgtatc aaaccgggcg
     3721 agacaacacc ggacaataaa ttctctctgg aaaccgtcaa ctgcgtgggc gcctgcgcgt
     3781 tggggcctgt
//
LOCUS       DAOOLB010000143         3683 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_30387_length_3683_cov_36.007442, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000143 DAOOLB010000000
VERSION     DAOOLB010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30387_length_3683_cov_36.007442"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1276
                     /locus_tag="PLF77_10805"
     CDS             29..1276
                     /locus_tag="PLF77_10805"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPC09387.1"
                     /translation="MILPDYYEFCCRVKIVAGHDALEKIPALLAEMGSKKPLIITDKG
                     VAAAGLVDIVVNALKDGVTIGGIADNVPPDSDLKVVNQLAGLYRDNACDAIIAVGGGS
                     VMDTAKGVNIVVSENATDLMAFSGAGALNRRLKTLVAIPTTAGTGSEVTLVAVIKDHE
                     KHLKMAFTSYFLLPSVSILDSRMTMTMPPFITAMTGMDALSHAVEGYYCLQKNPLSDA
                     HSLAAVNMIHHYLPTVTKNPADKDGRLAMAVSACLAGISFSNSMVGMVHNLGHATGSV
                     CGVPHGICMAILLPYGLEYNMHKRGDTIGELLLPMAGEDVYLKTSKADRADKVVSLIR
                     QMNQDLNEITGGRHARFFSEIKDRDGKPMVSREILPEIAKAALLDGATIYNPEDSDYE
                     DNLLVLEAAWEGKPLDRNKIKKG"
     gene            1298..3253
                     /locus_tag="PLF77_10810"
     CDS             1298..3253
                     /locus_tag="PLF77_10810"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HPC09388.1"
                     /translation="MSKAYMGKILMVDLSSKTMTEETIPDSVYQQYLSGMGLGAYILY
                     NRIPAGADPLGPDNILGFCSGLLTGTGSLFSGRWTLVGKSPLTGGWGDANCGGTFSPA
                     IKHCGYDAIFFKGISPNPVYLYVKNGKTFLKDASDLWGMDAVETEEKLKKQYGERAGV
                     AVIGPAGEKVSLISGVCTDKGRIAARSGLGAVMGVKKLKAVVLDGVQRIQAQDPKSMK
                     SLSQKCIKWTNFPMPLGLLPGVGLALMGTFMGATPVMFAQDGLLYKTILRTWGTGGMN
                     QMSVEMGDSPLKNWDGSSRDFHLGKSKTINPDIIKKAEIIKYHCYSCPLGCGGICATK
                     GKYSHTHKPEYESILALGGLCMNEDLDSLFYLNELLNRAGMDTISAGATVAFAIECYE
                     KGILTKEDTDGLELKWGNTEAIVALIRKMVNREGIGDILADGSKVAAAKIGRGAEECT
                     VHAGGQEPAMHDGRGDPGFNVHYSVEPTPGRHTIGAHLYYEMFQLWKRVKGLPKPNLL
                     YMKGSKYKINKEKAEASAACSKFMSMGNGAGMCMFGLFIGAKRIPTFDWLNAATGWTF
                     TPEEYMTIGERIQTIKQNFNIRHGIEPRKNFISSRAQGKIPQKKGPNKGRFVDVEKLA
                     ADYWEQFGWDRTNGKPKEETLKKLGIK"
     gene            3265..>3683
                     /locus_tag="PLF77_10815"
     CDS             3265..>3683
                     /locus_tag="PLF77_10815"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPC09389.1"
                     /translation="MSYTITEKCNGCGACARVCPADAITGEKKKIHSINAALCIECGA
                     CGRVCPQGGVLDADGVLCVMIKRSEWPKPEIIVEKCIACGICVGTCPAGCLAMSDRPR
                     NQGVDAFPYLKDAKSCIGCGFCALECPVDAIVMQKPE"
BASE COUNT          947 a          886 c         1027 g          823 t
ORIGIN      
        1 caaggcctga acggaaagga tataacccat gatactaccg gattattatg aattttgctg
       61 ccgtgtaaaa attgttgcgg gacacgacgc gctggaaaag ataccggcgc tgcttgccga
      121 aatggggtca aaaaaaccgc tgatcatcac cgataaaggc gttgccgccg ccggtctggt
      181 ggatatcgtt gtcaatgctc tcaaggatgg tgttacgatc ggaggtattg ccgacaatgt
      241 tcctccggac tccgatttga aggtggtcaa ccagctcgcc ggtctttacc gggataacgc
      301 ctgtgacgcc attatcgccg tcggcggcgg ttccgtgatg gatacggcca aaggggtcaa
      361 catcgtcgtt tccgagaatg ccaccgatct gatggccttc agcggcgccg gggccctgaa
      421 tcggcgcctg aagactctcg ttgccatacc gaccacggcg ggaacgggat cggaagtcac
      481 actggttgcc gtgatcaaag atcatgaaaa gcatttaaaa atggccttta ccagttattt
      541 tttgttgccc agtgtatcca ttctcgattc ccggatgacc atgaccatgc cgcctttcat
      601 tacagccatg acgggtatgg atgccttgag ccatgccgtt gaaggatatt actgccttca
      661 gaaaaatccg ttgagtgacg cccactctct ggctgccgtc aatatgattc accactatct
      721 gcccacggtg accaaaaatc ctgcggataa agacggtcgc ctggccatgg cggtaagcgc
      781 gtgtcttgcc ggcatctctt tctccaattc catggtcgga atggtccata atctcggtca
      841 tgccaccggt tctgtctgtg gagttcctca tggaatctgc atggccattt tattgcccta
      901 tggtctcgaa tacaatatgc ataagcgcgg ggataccatc ggggaactgc tgctgcccat
      961 ggcgggcgag gacgtctatc tcaagacctc caaggcggat cgggcggata aggttgtttc
     1021 cctgattcgg cagatgaatc aggatctcaa cgagatcacc ggcgggcgac atgcgcggtt
     1081 cttctcggag atcaaagacc gcgatgggaa gccgatggtt tcacgggaga ttcttcctga
     1141 aattgccaaa gcagccctgc ttgacggagc aaccatttat aatccggaag attcagacta
     1201 tgaagataat ttattagtgt tggaagcggc atgggaaggc aagccccttg accggaataa
     1261 aattaaaaaa gggtaaacat tacaagagag agtttttatg agcaaagctt atatgggaaa
     1321 aatcctcatg gtggatctgt ccagcaaaac catgacggaa gagacaatac cggatagtgt
     1381 gtaccagcaa tatctgtccg gcatggggct cggagcctac atcctttaca accgaatacc
     1441 ggccggcgcc gaccctctgg ggccggacaa tattctgggc ttctgttccg gtctgctgac
     1501 cggcacaggc agtttatttt caggacgatg gacgctggtc ggcaagtcgc ctctgaccgg
     1561 aggctggggc gacgcgaatt gcggcggcac gttttctccg gccatcaagc attgcggcta
     1621 tgacgccatt ttcttcaaag gcatcagccc caatccggtt tatttatatg taaaaaacgg
     1681 caagacgttt ctcaaagatg cctcggacct gtggggaatg gatgccgtcg aaacggagga
     1741 gaaactcaag aaacagtacg gcgagagagc gggcgtggcg gtcatcggcc ctgccggaga
     1801 aaaggtttct ttgatttccg gtgtttgtac cgacaaaggc agaatcgccg cccgatcggg
     1861 attaggcgct gtcatggggg tgaaaaaact taaagcagtg gtccttgacg gtgtgcaacg
     1921 gattcaagct caagacccta aatcaatgaa aagtctgagt caaaaatgta tcaagtggac
     1981 caacttcccc atgccgctcg gcctgcttcc gggcgtgggc ctggctctta tgggaacatt
     2041 tatgggggcg actccggtca tgtttgccca ggatggtctc ctgtacaaaa ccattttaag
     2101 aacatggggt acgggcggca tgaatcaaat gtctgttgag atgggcgatt cgccgctgaa
     2161 aaactgggat ggttcaagtc gggatttcca cctgggtaaa tccaaaacca tcaatccgga
     2221 tatcattaaa aaggcggaaa tcataaaata tcactgttat tcgtgtccgc tgggatgcgg
     2281 cggtatttgc gccaccaaag gaaagtattc ccatactcat aaacccgaat atgaaagcat
     2341 cctcgcgttg ggagggttgt gcatgaatga agatctggac agcctctttt atttgaacga
     2401 acttcttaac cgggcgggca tggacaccat ttccgcgggc gcaacggtgg ccttcgccat
     2461 tgagtgctac gaaaaaggta ttctgacgaa ggaagatacg gacggtttgg aattgaaatg
     2521 gggcaatacg gaagcgattg tcgccctcat caggaaaatg gtcaaccggg aaggcatagg
     2581 cgatattctg gccgacggat ccaaagttgc cgcggcaaaa atcggccggg gcgcggaaga
     2641 gtgcactgtt catgcgggag gtcaggaacc ggccatgcat gacgggcgcg gcgatcccgg
     2701 cttcaatgtc cattacagtg tggaacccac gccgggtcgg catacgatcg gcgcgcattt
     2761 atattacgaa atgtttcagc tctggaagcg ggtcaaagga ttgcccaagc ccaatcttct
     2821 ctatatgaaa ggcagcaaat ataagatcaa taaggagaag gccgaagctt ccgccgcatg
     2881 cagtaaattt atgagcatgg ggaatggcgc gggcatgtgc atgttcggcc tttttatcgg
     2941 agcgaaaagg attcccacgt tcgactggct aaatgccgcg accggctgga cgtttacccc
     3001 tgaagaatac atgaccatcg gcgaacggat tcagacgatc aaacagaatt ttaacatccg
     3061 gcacggcatc gaaccgagaa aaaactttat cagcagccgc gcacagggga agattcctca
     3121 gaaaaaaggt cccaataagg gacggtttgt cgatgtggaa aaacttgccg cagattactg
     3181 ggaacagttt ggttgggaca ggaccaacgg caaacccaaa gaggaaacat tgaagaaact
     3241 gggaatcaaa taagaggtaa cagcatgtca tatacaatta cggaaaaatg caatggctgt
     3301 ggcgcctgcg ctcgagtgtg tcccgcggat gccattaccg gtgaaaaaaa gaaaattcat
     3361 tcgatcaacg cagcgctatg catcgaatgc ggcgcctgcg gcagggtttg tcctcaggga
     3421 ggcgtcttgg atgccgatgg cgttttgtgc gtcatgatca aacgatctga atggcccaag
     3481 ccggaaatca tcgtggaaaa atgtatcgcc tgcggcatct gtgtcgggac ctgtcccgct
     3541 ggatgcctgg ctatgtcgga ccggccgcga aatcaaggcg tggacgcttt cccatatctg
     3601 aaggacgcca aatcctgcat cggttgcgga ttctgcgctt tggaatgtcc ggtagatgcc
     3661 atcgtcatgc aaaagccgga aaa
//
LOCUS       DAOOLB010000144         3418 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_33516_length_3418_cov_64.189117, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000144 DAOOLB010000000
VERSION     DAOOLB010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33516_length_3418_cov_64.189117"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..676
                     /gene="hisD"
                     /locus_tag="PLF77_10820"
     CDS             <1..676
                     /gene="hisD"
                     /locus_tag="PLF77_10820"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HPC09390.1"
                     /translation="VDKIVGPGNAYVAAAKKMVFGQVDIDMIAGPSEVVVIADAKANA
                     SFAAADMLAQIEHDEMAAALLLTPSETFAITVYREIRRQIKTSARKKIIEKSLARYCA
                     IIITGSLDEAVNIANRFAPEHLELMTQSPDKILKKVRNAGSVFLGSYTPEALGDYLAG
                     TNHILPTGGTARFSSPLGVYDFYKRMSVLSFSRKAFEKLSRPTADFARMEGLDAHAHS
                     VLIRGK"
     gene            1069..1848
                     /locus_tag="PLF77_10825"
     CDS             1069..1848
                     /locus_tag="PLF77_10825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HPC09391.1"
                     /translation="MISKNNKYPSVKKVTDEERIGIVRDIFSTITGSYDFLNHFLSLG
                     RDIAWRKFAAKKMVFPRSGKFLDIACGTGDLSIIACATHQDMSVTGADFVHPMVRLAN
                     AKVRKKRLSGRIKFIQANALNLPFADNTFNVTAMAFGIRNIPQKDRAMKEMLRVTVPG
                     GQIMILEMTFIQNRLFKAAYYLYLNFLLPLLARLFSKNAAAYHYLADSIMNFPKPEAF
                     AVFMEKTGVTDVQIFPRTFGITCLYLGEKPAQNNRATQNHD"
     gene            complement(1859..>3418)
                     /gene="polA"
                     /locus_tag="PLF77_10830"
     CDS             complement(1859..>3418)
                     /gene="polA"
                     /locus_tag="PLF77_10830"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPC09392.1"
                     /translation="MAPAFNHTGIKKYFYDLKTALVCLDTLAVCSGDDLQLAAYLLNP
                     AKSSYELDDIVWEYLHEKIPSLNEIAGGKGKMYPLSLAPLDKMAAAAGQRAEAILDLA
                     GVLAGRLEKIDVLDLYRKVEMPLLHVLAEMEKKGVLLDLALLKQMSAELTQLLALSES
                     KIHHLAGEKFNINSPKQLQHILFEKLKLPAGKKTKEGYSTDMDVLTELARSHEMPAEV
                     LAYRSLAKLKSTYVDALPALVNPQTGRIHTSYNQTVTATGRLSSSNPNLQNIPIRTLE
                     GKRIRQAFIADPDCFLISADYSQIELRVLAHLSEDDALIDAFTSDEDIHSRTASDVFG
                     VFPQMVNPDMRRQAKVINFGILYGMSAFGLAKELGVSMKTAQEYIDGYFNRYKKVRLY
                     LDGILEGARQNGFVSTLLNRRRYLPELKSKIPSVRQFAERMAINAPIQGTAADLIKVA
                     MVNIAKLLLKKKLSARLIMQVHDELVIEAPAGEKADVMALVKKEMEEVIRLKVPLKVE
                     IASGKNWDEAH"
BASE COUNT          846 a          860 c          845 g          867 t
ORIGIN      
        1 ggtggacaaa atcgtcggtc ccggaaacgc ctatgtcgcc gcggcgaaaa agatggtctt
       61 cggtcaggtg gatattgata tgattgcggg acccagcgaa gtggtggtta ttgccgacgc
      121 caaggccaat gcgtcttttg cggcggcgga tatgctggcg caaatcgagc atgatgaaat
      181 ggcggcggcg cttcttttga cgccatccga aacattcgcc ataacggtgt accgggaaat
      241 caggcggcag attaaaacct cggcacgaaa aaagattatc gaaaaatcgc tggcccggta
      301 ctgcgccatt atcatcaccg gaagccttga tgaagccgtg aatattgcga atcgctttgc
      361 gcccgaacat ctggagttga tgacccaatc gcctgataaa attttaaaaa aggtcagaaa
      421 tgccggatct gttttcctgg gctcttatac gccggaagcc ctgggcgatt atctggcggg
      481 aaccaatcac attctgccca cgggcgggac agcccgtttt tcatcgccgc tgggcgtata
      541 tgatttttac aaacggatga gcgttctttc tttttccaga aaagcgtttg aaaaactgag
      601 caggccgacg gccgactttg cccggatgga gggtctcgac gctcatgccc attctgttct
      661 gattcgcggc aaataacagt tatccaaaag atttgattga tatcatgatt tattccgatg
      721 ctctggtgga cattaccgat aaaaacattc ttttaaaaaa ttacagtttc ttcaaccgca
      781 acagattggt ttttttcacg acatcgaaaa attgtcatca aaaaaccttc cgtccggacg
      841 ggcaaattcc gctttcacgg aaccggggat ttccacgcct gggttgcgaa agattttgaa
      901 agacacaaac gcgacaggat gttcatcgct tttatcccca gccaacggtg gcggatcgga
      961 tttaaggttg agcgttcaga tgccgttctg aaaatctttc aggaaagagg tttgatcaga
     1021 ccggcgggtt aagcatcctt tgttgacgaa tcgaaagaaa gtttgcagat gatttcaaaa
     1081 aacaacaagt atccttccgt aaaaaaagta accgatgagg aacgcatcgg catcgtcaga
     1141 gacatatttt cgaccattac cggcagttat gattttttaa atcatttttt gagcctgggt
     1201 cgcgacatcg cctggcggaa atttgcggca aagaaaatgg ttttcccccg ttccgggaag
     1261 tttctcgaca tcgcctgcgg aacgggagac ctgtctatca ttgcctgcgc cacgcatcag
     1321 gatatgtcgg ttaccggagc cgattttgtc catccgatgg tccgcctggc aaacgccaag
     1381 gtccggaaaa aaagactcag cggcaggatc aaattcattc aggccaacgc actgaatctt
     1441 ccgttcgccg acaatacctt taacgttacg gcaatggcct tcggaatcag gaatattcct
     1501 caaaaagacc gggcgatgaa agagatgctt cgcgtcacgg tgccgggcgg gcaaatcatg
     1561 attctggaaa tgacctttat tcaaaaccgc cttttcaagg ccgcctatta tctctacctg
     1621 aattttctgc ttccgctgct tgccagactc ttctctaaaa atgcggcggc ctatcactac
     1681 cttgccgatt ccatcatgaa ctttcccaaa cccgaagcgt ttgcggtatt catggaaaaa
     1741 accggcgtta ccgacgttca aatttttccg cggacgttcg gcatcacctg tttatatctc
     1801 ggggaaaaac ctgcccaaaa caatcgcgct actcaaaatc atgattgaaa atttctgatc
     1861 agtgcgcttc gtcccagttt ttgccggagg caatttccac tttcaacggc actttcagcc
     1921 tgataacctc ttccatttct tttttgacca gcgccatgac gtccgctttc tcacctgccg
     1981 gcgcttcaat aaccagttca tcatgtacct gcatgatcag gcgggcagac aacttctttt
     2041 tcagaagaag ctttgcgata ttcaccatgg ccaccttaat caaatcggcg gccgttccct
     2101 gaatgggggc attgatcgcc atgcgttcgg cgaactggcg cacggaagga atcttgcttt
     2161 ttaattccgg cagatagcgg cgccggttga gcagcgtgga aacaaagccg ttttggcgcg
     2221 ccccttccag aataccgtcc agataaaggc gcactttttt atagcggtta aagtatccgt
     2281 caatgtattc ctgagcggtt ttcatggaca cacccagttc ttttgccaga ccgaaagcgc
     2341 tcattccata aagaatgccg aagttgatca ctttggcctg ccggcgcatg tccgggttca
     2401 ccatctgggg gaaaacacca aagacatcgg aggccgtccg gctgtgaata tcttcatcgg
     2461 aggtaaaggc atcgatgagc gcgtcatctt cagacagatg cgcgagcacc cgtaattcga
     2521 tctgcgaata atcggcggag atcagaaagc aatccggatc cgcaataaag gcctggcgga
     2581 ttctttttcc ctcaagcgtt ctgatcggga tattctgcag gttgggattg gagctcgaca
     2641 accggcccgt cgccgtcacc gtctggttat aggacgtgtg gattctgcct gtttgcggat
     2701 tgaccaaggc cggtaaagca tccacataag tggatttgag cttggccagc gagcggtaag
     2761 ccaggacttc cgccggcatt tcgtggctgc gggcaagttc ggtcaacacg tccatatcgg
     2821 tggaataacc ttcctttgtt tttttcccgg cgggcaattt caatttttca aacaaaatat
     2881 gctgcagctg tttgggggaa ttaatattga atttttcgcc ggccaggtga tgaattttcg
     2941 attccgacaa cgccagcagt tgcgtaagct ccgccgacat ctgctttaac agcgccagat
     3001 cgagaagcac gccttttttc tccatctcgg ccagaacatg cagtagcggc atttccacct
     3061 tgcggtaaag atccaggacg tctatttttt ccagccggcc ggcgagcaca ccggcaagat
     3121 ccaggatggc ttcggcgcgc tgtcccgcag cggcggccat tttatcgaga ggcgccagcg
     3181 acagcggata cattttgcct ttgcctccgg cgatttcatt cagggacgga attttttcat
     3241 gcagatattc ccagacgatg tcatcgagtt cataagacga ttttgcggga ttgagaagat
     3301 atgccgccag ctgcaaatcg tcgccggagc aaactgcaag agtgtccaga caaacaagcg
     3361 cggtttttaa gtcataaaaa tatttcttta ttccggtatg gttgaaggcg ggagccat
//
LOCUS       DAOOLB010000145         3361 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_34287_length_3361_cov_46.943134, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000145 DAOOLB010000000
VERSION     DAOOLB010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34287_length_3361_cov_46.943134"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PLF77_10835"
     CDS             <1..593
                     /locus_tag="PLF77_10835"
                     /inference="COORDINATES: protein motif:HMM:NF014801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HPC09393.1"
                     /translation="LAEKTAEPPEKFFDSLIQHVPAGSKGLMLQPYWTPGREIDPFAK
                     GSIIGFGDVHSRAYVYRALLEGLVYGLREGGELTQRKTKVPVTRLKVSGGGSQSDAAM
                     QITADIFGMAAERPHTFETSALGAAIDAAVGLKLYPDFPAAVKAMTRTGRVFEPILKN
                     HQIYDQLYKRVYLKMYERLQPLYKEIRDITGYPATL"
     gene            703..>3361
                     /locus_tag="PLF77_10840"
     CDS             703..>3361
                     /locus_tag="PLF77_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09394.1"
                     /translation="MPLAWNEIKSRALAFSKEWAGESSEDAEAKTFWDGFFNVFGITR
                     KRIASFEEPVKKLGNQYGYIDLFWKGVLIVEHKSRGKSLDKAYDQALGYFPGLKERDL
                     PKYVIVSDFARIRLYDLEENQQHEFELKDLHKNARLFGFIAGYQTHKIQEQDPVNIKA
                     AEQMGRLHDRMKDVGYSGHPLELYLVRLLFCLFAEDTGIFEKQQFKEYIEERTSEDGS
                     DLGHHLSTLFQVLNTPREKRLKNLDEQLTAFPYVNGKLFEELLPAAAFDSAMRQELLE
                     CCSLDWSRISPAIFGSLFQSIMDKQARRNLGAHYTSEENILKLIKPLFLDALWEEFEK
                     IKHNKNKLFEFHKKLRRLNFLDPACGCGNFLVIAYRELRLLELEILRASHSSDQSFLD
                     VHSLLHLNVDQFYGIEIEEFPAQIAQVALWLMDHQMNLLVSEEFGLYFVRIPLQTSST
                     IVCGNALAIDWKTVVSPWSVSYIMGNPPFVGKKEQTSEQKKEVVGIFNDVKGNGVLDY
                     VTCWYKLAIRQMKSNENIKAAFVSTNSIVQGEQVCVWGNWMRSQGAEIFFAHRTFQWS
                     NEARGKAAVHCVIIGFSLQNSFSKIIFYYDTPKSDPQAVKAKNINSYLADGPDVFITS
                     RTIPLSMDVPEMKYGSMPIDNGNLILSKEQMKELLACEPEAKSYIRQYMGGDEFINSI
                     VRYCLWLVDCPPSLLRKMKKVHERVEKNRKYRLGSGREATKKLAGKAALFGEIRQPGG
                     PYLIVPKVSSENRHYMPVGFCETKIIASGTTLIVPNAKMYHFGVLQSEMQMSWMRQVC
                     GRMKSDYQYSAGIVYNNFPWPENPTEKQKQAIEAAAQGVLNARAQFPGSSLADLYDPL
                     TMPPALLKAHQTLDRAVDAAYGKTNFKTEA"
BASE COUNT          906 a          866 c          840 g          749 t
ORIGIN      
        1 tgcttgccga gaagacggca gagcctccgg aaaagttttt tgattcgttg attcaacatg
       61 tgccggccgg ttccaaaggc ctgatgcttc agccttactg gacgccggga cgggagattg
      121 atcctttcgc gaagggatcg attatcggct tcggcgacgt gcacagccgg gcctatgtgt
      181 atcgggcgct gctggaaggg ctggtctatg gattaaggga aggcggagaa ctgacccagc
      241 gcaaaaccaa agtccccgta accaggctca aggtgtcggg aggcggttcg caaagcgacg
      301 cggcgatgca gattacggcg gacattttcg gcatggcggc ggaacgaccg catacatttg
      361 agacctccgc cctgggcgcg gccatcgatg ccgcggtcgg ccttaaactt tatcctgatt
      421 tcccggccgc ggtcaaagcc atgacccgca cgggaagagt gtttgagccg attctgaaaa
      481 atcaccaaat ctacgaccaa ctatacaaga gagtttacct caaaatgtat gaacggctgc
      541 agccgctgta taaagagatc cgggacatta ccggttatcc cgcgacgttg tagaatggtt
      601 ttaccagccc cattgccgtc aatcagcccc caaacgaaaa ccgattgaaa caatggatca
      661 ttttcagtat agtcacggca attcaaagaa gaaccggaac ctatgccgct tgcctggaat
      721 gaaatcaaat cgcgcgcctt ggccttttcc aaagagtggg ccggggaaag ctccgaagac
      781 gcggaagcca aaaccttctg ggatggtttc ttcaatgtat tcggcattac ccgcaagcgc
      841 atcgccagct tcgaagagcc ggtcaaaaaa ctcggcaatc agtacggcta catcgatctc
      901 ttctggaaag gcgttttgat tgtagagcac aaatcgcgcg gcaaaagcct ggataaagcc
      961 tacgatcagg cgctgggcta cttccccggc ctgaaggaac gcgatcttcc caaatatgtc
     1021 atcgtctctg atttcgcccg aatccgtctg tacgatctgg aagaaaacca gcagcatgaa
     1081 tttgagctga aagacctgca caaaaacgcg cggctgttcg gctttatcgc cggataccag
     1141 acgcataaga ttcaggagca ggaccccgtc aacatcaaag ccgccgagca gatgggcagg
     1201 ctccacgacc ggatgaaaga cgtgggttac agcggccacc cgctggaact ttatctggtc
     1261 cgcctcctct tctgtctgtt tgccgaagac accggtattt ttgaaaagca gcaatttaaa
     1321 gaatacatcg aagagcgcac ctccgaggac ggatccgatc tgggccatca cctgagcacg
     1381 ctgtttcagg ttttaaacac ccccagagaa aaacgcctga aaaatctcga tgagcaactg
     1441 accgcctttc cctacgtcaa cggcaaactc tttgaagaac tgcttccggc ggccgcgttc
     1501 gattccgcca tgcgccagga gttactggag tgctgctcgc tggactggag ccgcatctcg
     1561 cccgcgatct tcggctcgct tttccaatcc atcatggaca agcaggcccg gcgcaacctg
     1621 ggcgcccact acaccagcga agaaaatatc ctcaagctga tcaagccgct gtttctggac
     1681 gcgctttggg aagagtttga aaagatcaaa cacaacaaaa acaagctctt tgaatttcac
     1741 aaaaagctgc ggcgcttgaa ttttctcgat cccgcctgcg gctgcggcaa ctttctggtc
     1801 atcgcctacc gtgagcttcg gctgctggag ctggaaattc tgcgcgcgtc gcactcaagc
     1861 gaccagtctt ttctggatgt ccattcgctc ttgcatctga acgtcgatca gttctacggc
     1921 atcgaaattg aagaatttcc cgcgcaaatc gcgcaggttg ccctgtggct gatggaccac
     1981 cagatgaatc tgctggtttc cgaggaattc ggcctctact ttgtccgcat ccctctgcaa
     2041 acgagcagca ccatcgtttg cggaaacgcc ctcgccatcg actggaaaac cgtcgtctca
     2101 ccctggagcg tcagctacat catgggcaac ccgccgtttg tggggaaaaa agagcagacc
     2161 agcgaacaaa aaaaggaggt cgtaggtatt tttaatgatg tcaaaggaaa tggagttctt
     2221 gattatgtga catgctggta caaattggca attaggcaaa tgaagtccaa tgaaaatatt
     2281 aaagcagcct ttgtttcaac caattcaatt gtgcaaggag aacaggtgtg tgtatggggc
     2341 aactggatgc ggtcacaagg agctgaaata ttttttgccc accgaacgtt tcaatggagc
     2401 aatgaggcac gtggcaaagc ggcagtgcat tgtgtgataa taggcttcag tttgcaaaac
     2461 agcttcagta aaattatttt ttactatgac actcccaaat ctgatcctca agccgtaaaa
     2521 gccaaaaata tcaattccta tctcgccgat gggccagatg tctttattac gagtcgaaca
     2581 attcctctga gtatggatgt gccggaaatg aaatatggca gcatgccaat cgacaacgga
     2641 aatttgatat tatcaaaaga gcaaatgaaa gaacttctcg cttgtgaacc ggaagccaaa
     2701 agctacatac gacaatatat gggtggagac gaattcatta acagtattgt gcgttactgt
     2761 ctttggttag ttgactgtcc acccagcctt cttcgcaaaa tgaagaaggt tcatgaacgc
     2821 gttgaaaaaa atcgcaaata tcgtttgggt agcggtcgtg aagctacaaa aaaattggcg
     2881 ggaaaagcgg cattgtttgg agaaattaga caacctggcg gaccttatct catcgtgccc
     2941 aaagtatcat cagaaaatag acattacatg ccggtcggat tttgtgaaac gaaaatcatt
     3001 gccagcggca ctacgctgat agtgcctaat gcaaagatgt atcactttgg agttctccaa
     3061 tcggaaatgc aaatgtcatg gatgcgtcaa gtatgtggcc ggatgaaaag tgattatcaa
     3121 tattcagcag gcatcgttta caacaacttc ccctggccgg aaaatccgac ggagaagcaa
     3181 aagcaagcca ttgaagccgc cgcgcagggc gtgctcaatg cccgcgcaca atttcccgga
     3241 tcgtccctgg ccgatcttta cgacccgctc accatgccgc ccgccctgct caaagcccat
     3301 cagacgctcg accgcgccgt cgatgccgcc tacggtaaaa ccaattttaa aaccgaagcc
     3361 g
//
LOCUS       DAOOLB010000146         3354 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_34381_length_3354_cov_37.463474, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000146 DAOOLB010000000
VERSION     DAOOLB010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34381_length_3354_cov_37.463474"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            233..1201
                     /locus_tag="PLF77_10845"
     CDS             233..1201
                     /locus_tag="PLF77_10845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPC09395.1"
                     /translation="MASVLMDIQQLKKTYVLGGGFMGHKPRVIRAVNGVDLQIFKGET
                     LGLVGESGCGKSTLARLLMKLEEPDEGSVSFRDRDITSLSSEDVKTYRRRVQMIFQDP
                     YSSLNPRKSAQSIISEPLTIFRMSSRAERKEKVLQLMKKVGLSEEQAHRYPHEFSGGQ
                     RQRIGIARALALQPELIIADEPVSALDASIQAQILNLLSDLKKDFGLTYLFISHDLNV
                     IHHVSDRIAVMYLGRIVELADKNELYREPLHPYTRMLMAAMPGCDPIRKKKEMPVRGE
                     AGDAGDGGCSFKNRCMYKIAVCDEKVPELRSYPGRGLCACHRTGEI"
     gene            1550..2170
                     /locus_tag="PLF77_10850"
     CDS             1550..2170
                     /locus_tag="PLF77_10850"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPC09396.1"
                     /translation="MHHEEKVIWKNGSLTGAGLWHQGSVDACAVICHPHPQMGGSMHN
                     NVVETVRDEWIAAGFSTLRFNFRGVGESTGHYDEGRGEKQDLLSACAFAKSRGVEKIF
                     LAGYSFGAWICCRLLKDHPSVSSFVVLISPPLKYFEFDWTGLENAVELMICGDQDMFC
                     DAEDLRKKAELIFSKWTILPGVDHFYFGQEARLAERLRQYICEKKA"
     gene            2396..2596
                     /locus_tag="PLF77_10855"
     CDS             2396..2596
                     /locus_tag="PLF77_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HPC09397.1"
                     /translation="MAEGKVKWFNEKKGFGFIENDEGGDVFVHYSAIQGTGFKTLYEG
                     QRIRFDIEQGNKGPSAANVQPL"
     gene            2661..>3354
                     /locus_tag="PLF77_10860"
     CDS             2661..>3354
                     /locus_tag="PLF77_10860"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPC09398.1"
                     /translation="MKKNLIVILGPTASGKTAMAARLAAEFGGEIISADSRQVYRGMD
                     IGTGKDLEEFRIDGRVIPCHLIDILDPDQEFNVFEFQKRFYEIFSALREKQVLPLLVG
                     GTGLYLESVLTRYFMPNAETDERMREELAGLTMEELQDILLAMKPQLHNQTDLHDRER
                     LIRKIEIEKARQNSSNHSDPFPQIHAGVFGIFWERAELKKRISTRLRQRLAEGMIEEV
                     SRLHDEGVSWERL"
BASE COUNT          841 a          772 c          950 g          791 t
ORIGIN      
        1 ttgtctcctg cttcgtcgcc gagagacttg cgaaaagtta atgacgaacc ttattgtcat
       61 gactataaaa atagaatcaa ggcaaagctt tccggcgcac ctgaaggtac gccctacata
      121 gctcttttgt cattctcgcg caggcgggaa tccagaatat gcttaatcag tagattcctg
      181 cactcgtgcc ctttagggta ttcgcgagta atgacacatt gtcgaggtgt tcatggcttc
      241 cgtattgatg gatattcagc aactgaaaaa aacctacgtt ttaggcggcg gatttatggg
      301 gcacaagccc cgggtcattc gggcggtgaa cggcgtcgat cttcaaatct tcaaaggaga
      361 aacgctgggg ctggtcggcg agtcgggatg cggcaaatcc acgctggcgc gcctcctgat
      421 gaagttggag gagccggacg aagggagtgt ttctttccgg gatcgggaca ttacttcctt
      481 gtcatcggaa gatgtaaaga cgtatcgccg ccgcgtgcag atgatttttc aggatcctta
      541 ttcttccctg aacccgcgaa aatccgccca gagcatcatt tccgaaccgc tgacgatttt
      601 ccggatgtcc agccgcgccg aacggaaaga aaaagttctt caactgatga agaaagtcgg
      661 cctgagcgag gagcaggccc atcgctatcc ccacgagttc agcggggggc agaggcagcg
      721 catcggcatc gcccgggcgc tggcgcttca gccggagctg atcatcgcgg acgagccggt
      781 ctccgcgctc gacgcttcca tccaggcgca gattctgaat ttgcttagcg atctgaaaaa
      841 agatttcggt ctgacctatc tgtttatctc ccatgacctc aacgtcattc atcacgtgtc
      901 cgaccggatt gccgtgatgt atctggggcg catcgttgaa ctggcggaca aaaatgaact
      961 ctaccgggag cccttgcatc cgtacactcg aatgctgatg gcggccatgc ccggctgcga
     1021 tccgataaga aagaaaaagg agatgccggt tcgtggagaa gcgggagatg ccggggatgg
     1081 cgggtgcagt tttaaaaacc gctgcatgta taaaattgcc gtttgtgatg agaaggttcc
     1141 ggagcttcgg tcctacccgg ggcggggact gtgcgcctgt caccgcacgg gcgagatatg
     1201 atcccaaacc gtttgtgatt ttatagaaga gacaatgcgc tgtcattgcg agcaatttac
     1261 caggagctac gcaccaggtt ataaatggaa gaaactgtaa tctcccctcc ctcgatggga
     1321 ggggcagggg gagggtgaaa acaaatgctt ccccctcccc tcaatccccg cccaccaggg
     1381 gcggggaagt atcttaggag cagactcttg gtctgcgtgg caatctaaaa caggagattg
     1441 cttcactttg ctcgcaatga ccagttcttg ttatcgtgag aatacaaaac ttaacctgcg
     1501 tgccacctgg ttatggcgta atcttaaata ttcaaatgga gcaatgacca tgcatcatga
     1561 agaaaaagta atctggaaaa acggcagtct caccggagca ggtttgtggc atcaaggatc
     1621 cgtggatgcg tgcgcggtga tctgtcaccc gcatccgcag atgggcgggt ccatgcataa
     1681 caatgtcgtg gagactgtcc gcgatgaatg gattgccgcc ggcttttcca cgctgcgctt
     1741 caattttcgc ggcgtcgggg aaagcaccgg gcattatgac gagggcaggg gagagaagca
     1801 ggatctttta tccgcctgcg cgtttgcaaa aagccgcggc gttgaaaaaa tatttctggc
     1861 cggttattcg ttcggtgcgt ggatctgctg caggctgctg aaagatcacc cgtccgtttc
     1921 gtctttcgtt gtgttgattt ctcctcccct gaaatacttt gagttcgact ggacgggtct
     1981 ggaaaacgct gtggaattga tgatctgcgg ggatcaggat atgttttgcg acgcggagga
     2041 tcttcgaaag aaagctgaat tgattttttc aaaatggaca attcttccgg gggtcgacca
     2101 tttttatttt gggcaggaag cccgattggc tgagcgtttg aggcaatata tttgtgaaaa
     2161 aaaagcttga ttttaaaaaa aatggtgtta tgttcgggtc caacgcaccg gttttataac
     2221 cggtttcgca agtgttggaa ggttcgggtt ggtaaataag tagtgcagag atcgatttac
     2281 aaaaatagaa aataaccgca aagagttctt gttgcaaaaa gaattcttgc ggttttttta
     2341 gtttctgacg gaatcaaccg gaagaagcaa atatttgaaa ggggagggat tagcagtggc
     2401 agaaggcaaa gtgaagtggt ttaatgagaa gaagggtttt ggcttcattg aaaatgatga
     2461 aggtggggat gttttcgtac attacagcgc cattcaggga acgggcttca agaccctcta
     2521 cgagggacag aggattcgtt tcgacatcga gcaggggaac aagggcccca gcgcggcaaa
     2581 cgttcagcca ctttagtcgc tgaaaaagat gaacaaaaaa atgccccgtg atgcggggca
     2641 ttttttttga tccgtacatc atgaaaaaaa atctgatcgt catcctggga ccgacggcat
     2701 cgggaaaaac ggcgatggcc gcaagactgg ccgccgagtt tggaggtgaa atcatttccg
     2761 cggattcgcg gcaggtttac cggggcatgg acatcggcac cggcaaggat ctggaagaat
     2821 tccggatcga cggccgcgtt attccctgcc atctgatcga cattttggac ccggatcagg
     2881 agtttaatgt ttttgagttt caaaagcggt tttatgaaat tttttccgcg cttcgggaaa
     2941 aacaggttct tccccttctg gtcggcggca cgggcctgta cctggaatcc gtcctgaccc
     3001 gatacttcat gccgaatgcc gaaacggacg agcgcatgag ggaagagctg gcggggctga
     3061 cgatggaaga gcttcaggat attctgctgg ccatgaagcc ccaactgcac aatcaaaccg
     3121 atcttcacga ccgggagcgg ctgatccgga aaatagaaat cgaaaaagcc aggcaaaact
     3181 cctcaaacca ttcagacccg ttcccgcaaa tccatgccgg agtcttcggt attttttggg
     3241 agcgggccga gctgaaaaaa agaatctcaa ccagactgcg ccagaggctg gccgagggga
     3301 tgattgaaga agtttcccgt ctgcacgatg agggtgtgtc ctgggaaagg ctgg
//
LOCUS       DAOOLB010000147         3350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_34433_length_3350_cov_29.800910, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000147 DAOOLB010000000
VERSION     DAOOLB010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34433_length_3350_cov_29.800910"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..2585)
                     /locus_tag="PLF77_10865"
     CDS             complement(204..2585)
                     /locus_tag="PLF77_10865"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HPC09399.1"
                     /translation="MNEAETRAEHIDPALKAAGWGVTEGSRIRREFPITPGRIEGLGR
                     RGKPLSADYVLEYRNTKMAVVEAKAWDEELTEGVAQAKNYADKLAVRFTYATNGKGIY
                     AIDMKTGKEGEIAAYPKPEELWKLTFAEENTWRDRFASVSFEDRGGSHPSRYFQDIAV
                     ERVMEAIAANKPRILLTMATGTGKTFVAFQIAWKLFHSRWNLSREPSRLPRILFLADR
                     NILADQAYNAFSAFPEDAMVRISPADIRKKGKVPKNGSIFFTIFQTFMSGPPIDGKPS
                     PYFGEYPPDFFDFIVIDECHRGGANDESNWRDILEYFAPAVQLGLTATPKRKDNVDTY
                     AYFGEPVYIYSLKDGINDGFLTPFKVRQISTTIDDYVYTPDDMIMEGEVQFGKIYLET
                     DFNKIIEIKEREAHRVKLFMDLIDQREKTLVFCASQDHALAVRDLINQMKTSKEPNYC
                     QRVTANDGALGEQHLRDFQDNEKTLPTILTTSQKLSTGVDARNIRNIVLMRPINTIIE
                     FKQIIGRGTRLYDGKDYFTIYDFVKAYQHFSDPEWDGEPLEPEPCSKCGCYPCACVKE
                     PPKPCPVCGQRPCVCPKEPCPKCGRRPCVCIKKAKVKLADGKERSIQHMMVTSFWHPD
                     GTPMSAQQFMEMLFGKLPEFFKDEEELRAIWSAPDTRIKLLRGLAEKGFGHQQMAEMQ
                     KIIDAEKSDLFDVLAYVAYALPPITREERARQAKVIISAHFNGKQQVFLDFVLSHYVS
                     VGVEELEQSKLTPLLRLKYHDSLADAVSDLGQPEEISRVFSGFQKYLYQSAAA"
     gene            complement(2693..>3350)
                     /locus_tag="PLF77_10870"
     CDS             complement(2693..>3350)
                     /locus_tag="PLF77_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HPC09400.1"
                     /translation="ENASFIPVAPEHLQQAMDDWERYLNITAELDALVQLAVLHVEFE
                     AIHPFKDGNGRLGRMLIPLFLYQRKLLGSPNFYMSAYLEANREEYQEKLRAVSREGNW
                     TEWCEFFLRGIQQQAAENERKARAILALYDRVKKQVSDWTHSQYSIKAVDFIFKSTIF
                     SAPQFTGHSSIPRPTAARILGILKDRGLLVPLRQGKGGRAGIFVFRDLFNIAEGRDVF
                     "
BASE COUNT          800 a          886 c          810 g          854 t
ORIGIN      
        1 agatgatcct cccgcgcatc atccgctcgc agatggtaaa ggggctggaa agacccaaag
       61 cctaaggaac ggtttgaacc accctttcgg gtggcgcaac cgttccctac actctgtgta
      121 tcccatattc aggatagaag gcgggggttt cccccgccat gttgcataat ccgtatcact
      181 gaattgcaag tcacaagaga actttacgct gctgcagatt gataaagata tttctgaaac
      241 cctgaaaaca cccggctgat ttcctccggt tgcccaaggt ccgacacggc gtcggccagg
      301 gaatcgtgat atttgaggcg caaaagaggc gtcagtttgc tttgctcaag ctcctcaaca
      361 ccgacgctca cataatgtga aaggacaaaa tccagaaaga cctgctgctt gccgttaaag
      421 tgcgcactga tgatcacttt tgcctgccgg gcgcgctcct cgcgtgtgat tggcgggagt
      481 gcataagcca cgtatgccag cacgtcaaat agatcgctct tttcggcatc gataatcttt
      541 tgcatctccg ccatctgttg atgaccgaag cctttttctg caagcccccg cagcaacttt
      601 atgcgggtgt cgggagcgct ccagattgcg cgcaattctt cctcatcctt gaaaaactcc
      661 ggcagcttgc cgaaaagcat ttccataaac tgctgcgccg acatcggagt gccgtcggga
      721 tgccagaagc tcgtcaccat catatgctga atgctgcgct cttttccgtc agcgagcttg
      781 actttagctt tcttgataca aacacaggga cgccggccac acttcgggca cggctccttc
      841 gggcagacgc agggtctttg accgcaaacc ggacagggct tcggcggttc tttaacacag
      901 gcgcagggat agcagccgca tttggaacag ggttccggct ccagcggttc accgtcccat
      961 tcgggatcgc tgaaatgctg ataggcttta acgaaatcgt aaatcgtaaa ataatccttg
     1021 ccgtcataga gccttgtgcc gcgtccgatg atttgtttga attcaataat cgtattgatc
     1081 ggccgcatga gcacaatatt gcggatgttg cgggcatcaa cgccggtgga aagtttttgc
     1141 gacgtggtca ggattgtcgg cagcgttttt tcgttgtcct gaaagtcccg cagatgctgc
     1201 tcgcccaggg ctccgtcatt ggccgtcacc cgctggcagt aattcggctc tttgctggtt
     1261 ttcatctgat tgatcaaatc gcgcacggcc agcgcgtgat cctggcttgc gcaaaagacc
     1321 agcgtctttt cccgctgatc gatcagatcc atgaacaatt ttacacggtg ggcttcacgt
     1381 tcctttatct cgataatctt attgaaatcc gtctccagat aaatcttccc gaactgaact
     1441 tctccctcca tgatcatatc atctggcgtg taaacataat catcaatcgt tgtggagatt
     1501 tgcctcacct taaaaggagt aagaaacccg tcgttgatgc cgtctttcaa tgaataaatg
     1561 taaaccggtt caccgaaata ggcatacgta tccacattgt cttttctttt gggggtggcg
     1621 gttagtccca actgaacagc aggcgcaaaa tattcgagga tatcccgcca gttgctttca
     1681 tcattggcgc ctccgcgatg acactcgtca atcacaatga aatcaaaaaa gtcaggggga
     1741 tattcgccaa aatatgggct gggctttcca tctattggcg gaccgctcat aaacgtttgg
     1801 aagatcgtga agaagatgct gccgttcttg gggactttgc ctttcttgcg gatgtccgcc
     1861 ggtgaaattc tgaccattgc atcttccggg aaagcggaaa acgcattata ggcctgatca
     1921 gccagtatat tccggtcggc caaaaagaga atgcgcggca ggcgcgacgg ctcgcggctc
     1981 aaattccagc ggctgtgaaa cagtttccag gcaatctgga aggcgacaaa tgttttacct
     2041 gttccggtgg ccattgtgag caggatgcgc ggcttattgg cggcgatggc ttccataacc
     2101 cgttcaaccg caatatcctg aaagtagcga ctcggatggg aaccgcccct gtcttcaaag
     2161 gaaacagacg caaaacgatc gcgccaggta ttctcctcgg caaaggtcaa tttccatagt
     2221 tcttccggtt tgggataagc ggcaatctcg ccttccttac ccgttttcat atcgatggca
     2281 taaatacctt ttccgttggt cgcgtaagtg aaacgaacgg ccaatttgtc ggcatagttc
     2341 ttggcctggg caacgccttc ggtcagttct tcgtcccatg cttttgcctc gacaacagcc
     2401 atttttgtgt tgcgatactc cagaacataa tcagccgata atggttttcc gcgccgccca
     2461 agaccttcaa tgcgtcccgg agtgattgga aattcccgcc ggatacggct tccctcggta
     2521 acaccccagc cggcggcttt gagcgccggg tcaatatgtt cggctctggt ttcggcttcg
     2581 ttcatggcgt tttagcaaaa agtgtttttg tctcacggat tgaatgcttt aagttaattg
     2641 tctcataata agcgatatat gagataaaat agccattttg tctcatcgtt gctcaaaaca
     2701 cgtcccttcc ctccgcaatg ttaaaaagat cacgaaaaac aaatattccg gcccgtcccc
     2761 ctttaccttg gcgcaatggc acaagcagcc cacgatcctt taggataccc aggatacggg
     2821 cagccgttgg tctgggaatt gacgaatggc cggtaaactg cggcgccgaa aatatggttg
     2881 atttaaatat aaaatcaact gctttgatcg aatactgaga gtgggtccaa tcgctcacct
     2941 gcttcttcac acgatcataa agggccagaa tagcccgtgc tttgcgttca ttttcagcag
     3001 cctgctgctg aatacctctg agaaagaatt cgcaccactc tgtccagtta ccttcacgcg
     3061 atacagcccg aagtttttcc tgatactctt cccggttcgc ttccaggtag gcgctcatgt
     3121 aaaaattcgg acttcccagc aatttacgct gatagagaaa aagcggtatc agcatccggc
     3181 cgagacggcc gttaccatcc ttgaagggat ggatagcttc aaactccaca tggagaacag
     3241 caagctgcac cagtgcatca agttcggcag tgatgttcaa ataacgttcc caatcgtcca
     3301 tcgcctgctg caagtgttcc ggcgctacgg gaataaaaga agcgttctct
//
LOCUS       DAOOLB010000148         3240 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_36052_length_3240_cov_45.735008, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000148 DAOOLB010000000
VERSION     DAOOLB010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36052_length_3240_cov_45.735008"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(102..>1066)
                     /locus_tag="PLF77_10875"
     CDS             complement(102..>1066)
                     /locus_tag="PLF77_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPC09401.1"
                     /translation="DKEQVRSQITGLGSLVTWEDDDLSCQTADFRHLNAEKTLHIITD
                     AAGSLTTQDAKIHGFTLLNSYLTIGDQCLPETFFHPEDLYRAMKKDIRVSTSQASVYE
                     RHQFYQSALERFDKVLYLCVGSVFTGNYQAAVQWKKDHDKEDRFIILDTGAASGRLGT
                     IALSTARYLAESRDVTNTIIFARKAIRLCEEYLFLDKLHYLAAGGRLSKTSAFFGDKF
                     NVKPVISPLPDGAKKVGTAKNQEDQITFALAKLDATIKDDSRALIMLEYSNNRRFVDG
                     TVRKNIKELYPDAEIILQPLSLTSGAHMGPGTWGVAFLPQINIR"
     assembly_gap    1067..1166
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1167..1978)
                     /locus_tag="PLF77_10880"
     CDS             complement(<1167..1978)
                     /locus_tag="PLF77_10880"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HPC09402.1"
                     /translation="MNEVLKQSFTAGVERLMARSDLLDSINVFPVADGDTGRNLSLSL
                     MPLRNTDLPQATMIHRLLLSARGNSGNIASQFFSAFCAVENIADLAGRAREGKIKAWQ
                     ALVNPRRGTMLNVFDALADVLSEQFTPDKENIENIIRSMENAVHQTYEQLPKLQEAGV
                     VDAGALGIYIFFEGFFYTLTGLSEDGRPLTEIFHDRLHISSSFQENIESGYCVDFVVK
                     APRYSPDEIKMITSVQEEVIVIPEGEFYKVHLHTQDKEQVRSQITGLGSLVT"
     gene            complement(1980..2396)
                     /locus_tag="PLF77_10885"
     CDS             complement(1980..2396)
                     /locus_tag="PLF77_10885"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HPC09403.1"
                     /translation="MKEKQNSIDVKIRVPFFDLDPMQIVWHANYLNYFEIARAALFES
                     RGIDLYSFYEKHQLIFPIIKTSTKHVLPLFHRDEIICKATLVEARIKLIVDFEIRKAE
                     DGKIVARGRTEQVTVKVPEREMLFRIPEEISRAFGF"
     gene            complement(2393..>3240)
                     /locus_tag="PLF77_10890"
     CDS             complement(2393..>3240)
                     /locus_tag="PLF77_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HPC09404.1"
                     /translation="ALKGNARHYHPVISEAKPFPGQAFVAAQITRLLKAKISTRHLED
                     DSLDALQDPYSLRCTPQVAGVLYDGLEWIRTWVEIEANSSNDNPIFDPATGEILMGGN
                     FYGGHIAFAMDGLKAALASVADMCDRQIMLLVSPNLNRGLPGDLVRKPPHEKGLRHGF
                     KAMSISSSALTAEALKLTMPAASFSRSTESHNQDKVSMGTIAARDAERICTLTERVVA
                     IHLLAAAQACDIRDNTNARPLLSKTMGKIRSVSAPVIEDRAMDNDIENICTAIRYSDF
                     FKIKP"
BASE COUNT          719 a          799 c          827 g          795 t
ORIGIN      
        1 gccacctgcg ctaccgttcc ggcatgatta taaacgggaa tgacaaaagc aaaacggttt
       61 ttgttatgat cttttgacct ggtcaatgtt tgtttctcat tttaacggat gttgatttga
      121 gggagaaacg ccacccccca cgttcccggc cccatgtgcg cgccggacgt cagcgacagc
      181 ggctgaagta taatttcagc atcaggatac agttccttta tattttttcg aaccgtgccg
      241 tcgacaaatc gtctgttgtt ggaatattcc agcatgatca gcgcacggga atcgtctttg
      301 atcgttgcgt caagtttagc caaggcaaac gtgatctgat cctcctgatt tttggccgtg
      361 ccgacttttt ttgcgccgtc cggcagcgga gatatcaccg gctttacgtt gaatttgtcg
      421 ccgaaaaagg cgctggtttt ggaaaggcgt ccccccgccg ccagataatg caacttgtcc
      481 agaaacagat attcttcgca caggcgtatg gccttacggg caaaaataat ggtgtttgtc
      541 acgtcacggc tttcggccag ataccgggcg gtggacaaag cgatggttcc cagtcggccc
      601 gatgccgctc ccgtatccaa gatgatgaac cggtcttctt tgtcgtgatc ttttttccac
      661 tggacggccg cctgataatt gccggtaaat acggagccga cgcaaaggta caacacttta
      721 tcaaaacgct ccagcgcgct ttgataaaac tgatgccgtt cataaacaga ggcctgggac
      781 gtggataccc ggatgtcctt cttcatggcg cggtagaggt cttcgggatg aaaaaaggtt
      841 tccggcaggc attgatcgcc gatagtcaga taactgttta acagggtaaa accatggatc
      901 ttggcgtcct gagtcgtcag ggaaccggcc gcatcggtta taatatgcaa tgtcttttct
      961 gcatttaaat gcctgaaatc cgctgtttga caactcaggt cgtcatcttc ccaggtcacc
     1021 aaagagccga gccctgttat ttgagagcgc acctgctcct tgtcctnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnncagg tcaccaaaga gccgagccct gttatttgag
     1201 agcgcacctg ctccttgtcc tgcgtgtgaa gatgaacctt gtaaaactcc ccttcgggaa
     1261 tgacgatcac ttcttcctgc acgctggtga tcatttttat ttcatcggga gaatagcggg
     1321 gcgccttgac cacaaaatca acgcagtaac cggattctat gttttcctga aaagacgatg
     1381 agatgtgcag ccgatcatgg aatatttccg tcagaggacg accatcttcg gaaaggcctg
     1441 tgagtgtgta aaagaatcct tcaaaaaaaa tatagattcc cagcgcgccc gcatcgacaa
     1501 caccggcctc ctgcaattta ggcagttgct cataggtttg atggaccgcg ttttccatgc
     1561 ttcggatgat gttttcaatg ttttctttat ccggtgtgaa ttgttccgac agtacgtcgg
     1621 ccagggcgtc aaaaacattc agcatggtcc cccgacgcgg attgaccagg gcctgccagg
     1681 ccttgatctt tccttcacgg gcacgtccgg ccaggtcggc gatgttttcc accgcgcaga
     1741 aggcggagaa aaactgtgag gcgatgttac cggaattgcc gcgcgccgac agaagcagcc
     1801 gatgaatcat cgttgcttgc ggcagatcgg tgttgcgcag gggcatcagg ctaagggaaa
     1861 gatttctgcc ggtgtcgccg tcggcaaccg gaaaaacatt gatggaatcc agcagatcgg
     1921 agcgggccat aagacgttcc acaccggccg tgaaggattg tttgagcacc tcattcattt
     1981 cagaaaccaa atgcccggct gatttcttcg ggaatgcgaa acagcatttc tctttccgga
     2041 acttttacag tgacctgctc cgtgcgtcca cgggcaacaa tcttcccgtc ctccgctttt
     2101 cggatttcaa aatcgacgat cagtttaatg cgggcctcaa ccagcgtcgc tttacagata
     2161 atttcatcgc ggtggaaaag cggcaaaacg tgtttggtcg aggttttgat gatcggaaaa
     2221 atgagctgat gcttttcgta aaacgaataa agatcgatgc cgcggctttc gaaaagcgcc
     2281 gcacgggcga tttcaaaata gttcagatag ttggcatgcc agacgatctg catcggatcg
     2341 agatcaaaaa aaggaactct gatttttacg tcgatactgt tttgtttttc tttcatggtt
     2401 ttatcttaaa aaagtccgaa tatcgtatgg cggtgcagat attttcaata tcgttatcca
     2461 ttgccctgtc ttcgatgacc ggcgcgctga cggatctgat ctttcccatg gttttcgaca
     2521 gcagcggtct tgcgttggtg ttgtcccgga tgtcgcaggc ctgggccgcc gcgagaagat
     2581 gaatggcaac aacgcgttcc gtcaacgtgc agattctttc ggcatcgcgc gccgcgatgg
     2641 tgcccatgct gaccttgtcc tgattatgcg attcggtgga acgggaaaat gacgccgccg
     2701 gcatggttaa tttcagagct tccgccgtca gcgcggatga tgaaatggac atggccttaa
     2761 acccgtgccg caatcctttt tcgtggggcg gctttctcac caggtcgccg ggcagccctc
     2821 tgttcagatt cggactgacc agaagcatga tctggcggtc gcacatatcg gcgacagaag
     2881 ccagcgccgc ttttagtccg tccatggcga aggcgatgtg cccgccgtaa aaattgccgc
     2941 ccatcaggat ttcaccggtc gcgggatcga aaatggggtt atcgttggag ctgttggctt
     3001 cgatttccac ccatgtcctg atccattcga ggccgtcata caggacaccg gccacctgcg
     3061 gcgtgcagcg tagagagtaa ggatcctgca aggcatcgag ggaatcgtct tccaggtgcc
     3121 gcgtcgaaat ttttgccttc aacaggcggg tgatctgagc ggccacaaat gcctgaccgg
     3181 gaaaaggctt tgcttccgaa atcacgggat gataatgccg ggcattgcct ttgagcgcat
//
LOCUS       DAOOLB010000149         3138 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_37623_length_3138_cov_33.602984, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000149 DAOOLB010000000
VERSION     DAOOLB010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37623_length_3138_cov_33.602984"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1729)
                     /locus_tag="PLF77_10895"
     CDS             complement(170..1729)
                     /locus_tag="PLF77_10895"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPC09405.1"
                     /translation="MEYFNILNLKKEPFSNSPEPEFLFQLPQYSDCLQKLELAVRLRR
                     GINVIIGDIGTGKTTLCRKLIQNFSPSSKDAGKTEIEPHLLLDPAFKSNLEFLQTFAS
                     MLGINDSEPETESEWHLKEKIKDCLFDKGVDEKKIVVLMIDEGQKIPDDCLETLREFL
                     NYETNEFKLLQIIIFAQKEFYDILKKKANLLDRVNLLYRLRPLNFKQMKAMINYRLKV
                     AGHFEKHPPVFSFSGFLAVYLATGGYPRKAVFLCHQVLLKMIIREEKKAGWFLVRSGR
                     ENMKAPLSRKLGWGLAGLLFLIVLGISAGAVALQGLHSGKEKQGRLASTGSGINESAP
                     AMSVVHPSGDSTVQAMDVPDEKMPDALGKISMAKRRTLWWVSYNIYGDNTPQFMEKIF
                     AVNPHIKNKDVVAEGTIITLPAVPADSSPLNPGDILIALESGQDLEIMYNLFRNHPHE
                     QILPPLAFLSLWNKKKGFEFAVVINKCFKNEEEASQAINKLSPSVAAKAKILSQWDAD
                     TVFFNRRAPEY"
     gene            complement(1865..>3138)
                     /gene="pilQ"
                     /locus_tag="PLF77_10900"
     CDS             complement(1865..>3138)
                     /gene="pilQ"
                     /locus_tag="PLF77_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HPC09406.1"
                     /translation="MRQADLKAVLRAMAKSVNKNLLVKNELKGEISVDFKGVAWDEAF
                     TGLLRTYGLSYVWEGDIIRVMTADDLKQELERKVQMRDIQWVEPLLEPVVVSVDYADS
                     KKLAETAQELLTKDKDGKPRGSIKVDDHSNSLIISAMRDDLTKILPIIEKLDKPTSQI
                     LIKANIVETTKGVARDLGVMWGGYNRSTQGKEDLIITGGGVSPAGPIGGTGTAPQRSG
                     LGVNFPVDTTNLSSVIAPMGSLGLLFGRIDGNLLEVQLQALQQDNKLNILSSPSITTL
                     DNQEASTKNGERIPYVTTETSGGTTTQTTKFEDVVMELKITPHVIDGKNLKMKILVKK
                     DEVDMTRRSSMGDPYIIKKQTETELIVKDGETIVISGLTKQTNTYSQSGVPWLKDIPV
                     LGWAFKSDGKGENMEEVLIFITPHILPVGNS"
BASE COUNT          765 a          790 c          666 g          917 t
ORIGIN      
        1 aatctgtttt tccttgacaa tgccctgccg tattaaatac tgaccgaatt tcagattggt
       61 tttccgttgt tcacttaata ctttggcaag catttcttcg cttaaaatac cttggttgac
      121 cagtacctca cccaatcgtt ttctggtttt catgcatccc ccaatcagat caatattccg
      181 gcgcgcggcg gttgaaaaaa accgtgtccg cgtcccattg agataaaatt ttcgctttcg
      241 ccgccacaga tggcgacagt ttgtttatag cctgcgatgc ctcctcctca ttcttaaagc
      301 atttattaat cacaacggca aactcaaagc ctttcttttt attccacagc gaaagaaatg
      361 ccaatggcgg cagtatttgc tcatgaggat gattgcgaaa aagattatac ataatttcca
      421 gatcttgtcc actttctaaa gcgatcagga tgtcgcccgg attcaaggga ctggagtcgg
      481 cgggaacggc cggcaaagtt attatcgttc cttccgcaac aacatccttg tttttaatat
      541 gaggatttac ggcaaaaatc ttttccatga attgaggcgt gttgtcgccg taaatattgt
      601 atgacaccca ccagagcgtt cttctttttg ccatagagat ctttcccaga gcatcgggca
      661 ttttctcatc cgggacatcc atagcctgaa ccgttgaatc cccggacgga tgaacaacgg
      721 acattgccgg tgcagattca ttaatgccgg aaccggttga agccaaccgc ccctgttttt
      781 ctttgcctga atggaggccc tgcagcgcaa ccgcaccggc tgaaattccc aaaacaatca
      841 aaaataaaag cccggcgagt ccccatccta atttccggga taggggcgct ttcatgttct
      901 cccggccact tcgcaccaga aaccacccgg ctttcttttc ttcgcgaatg atcatcttta
      961 aaagaacctg atgacacaaa aaaactgcct tgcgcggata tcctcctgtc gccagataaa
     1021 cagcaagaaa gcctgagaag ctgaacacag ggggatgttt ttcaaaatgg ccggccacct
     1081 tcagacggta attgatcatg gccttcattt gcttaaaatt taacggacga agccggtaaa
     1141 gaaggttaac ccggtccaga agattggctt tctttttaag aatgtcgtaa aattccttct
     1201 gcgcaaagat aatgatctgt aaaagtttga attcgttggt ttcgtaattc agaaattcac
     1261 gcagtgtttc caggcaatcg tcgggaattt tctgtccttc atcgatcatg agaacaacga
     1321 tttttttttc gtccacaccc ttatcgaaca ggcagtcttt aattttttcc tttaagtgcc
     1381 attcgctttc tgtctcgggt tctgaatcgt tgatgcccag catggacgca aacgtttgca
     1441 gaaattccag gttgctttta aatgcgggat ccagcaaaag atgcggttca atttccgttt
     1501 tgccggcatc cttcgaagac ggtgagaagt tctggatgag tttacggcat aacgttgttt
     1561 tgcccgtgcc gatatctccg atgattacat tgattcctcg acgcagacgg acagccagtt
     1621 ccagcttttg taaacagtcg ctgtactggg gaagctgaaa aaggaactcc ggttcgggcg
     1681 aattggaaaa cggctctttt tttaaattga gaatgttgaa atattccatt tttaacggcc
     1741 atgttaacgt ttcaataccg gccattgttt agcgacacgg ccgctccgcc ggtataatgc
     1801 cggttttgcc cgttttgtgg atgatgagtt atctgatgct caactacgta ccgaataacc
     1861 gatgtcaact gttgccaacc ggcaaaatat gcggcgtgat aaatataagc acttcctcca
     1921 tattttcacc cttgccatcc gatttgaacg cccagcccag aacgggaata tcctttaacc
     1981 aaggcacgcc gctttgtgaa tacgtattgg tctgcttggt caaaccggag atcacgatgg
     2041 tttcaccgtc tttgacaatc aattcggttt cagtctgctt tttgatgatg tagggatctc
     2101 ccatggaaga tcttcgcgtc atatcaactt catctttttt tactaaaatc ttcatcttta
     2161 aattcttacc gtcaatgaca tgcggtgtaa tcttcagttc catcacaaca tcttcgaatt
     2221 tggtggtttg cgttgtcgta ccgccgctcg tttccgttgt gacgtaagga attctttcac
     2281 cattttttgt agatgcttcc tggttatcca gcgttgttat ggacgggctg gataaaatat
     2341 ttagtttgtt atcctgctgc agggcctgta actgaacctc cagcaagtta ccgtcgatcc
     2401 tgccgaacaa caaccctaac gatcccatgg gagcaataac ggatgacaga tttgttgtat
     2461 ccacggggaa attcacaccc aatcccgatc gttgcggagc cgttcctgtg ccgccgatag
     2521 gcccggccgg agaaacaccg ccacccgtga tgatgagatc ttccttacct tgcgtacttc
     2581 tgttgtatcc gccccacatc acgcccagat ctctggccac gcctttggtg gtttccacga
     2641 tgttggcttt gattaaaatc tgagaggtcg gtttatccag tttttcgatg atcggcagta
     2701 tctttgtcag atcatcgcgc atggcggaaa tgatcaacga gttgctatga tcatcgactt
     2761 tgatggaccc gcgcggtttt ccatctttat cttttgtaag aagttcctgg gctgtttccg
     2821 ccagtttttt ggaatcggcg taatccacac tgacaacaac cggctccaaa agcggctcga
     2881 cccattgaat atcacgcatc tgaactttgc gttccaattc ctgtttaaga tcatccgccg
     2941 tcataactct gatgatgtct ccctcccaga cataagacaa accgtaggtc cgcaacaatc
     3001 cggtaaatgc ttcatcccag gcgacccctt taaaatcaac actgatttcc cctttcagct
     3061 cgtttttcac cagcaggttc ttgttcaccg atttggccat tgcccgcaac accgccttga
     3121 gatccgcctg gcgcatgg
//
LOCUS       DAOOLB010000150         3081 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_38535_length_3081_cov_50.833113, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000150 DAOOLB010000000
VERSION     DAOOLB010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38535_length_3081_cov_50.833113"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1607)
                     /locus_tag="PLF77_10905"
     CDS             complement(<1..1607)
                     /locus_tag="PLF77_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HPC09407.1"
                     /translation="MLVNAVHKEQRRMAAVNEKGILVDFNIEMPTKEPITGNIYKGKV
                     LKVERGLQAAFVDYGGSKDGFLPLKDVSHDYFTEPEKGQSNGKPALKVGQEIIVQAVR
                     EAIGNKGALLTSYISLPGRYLVLLPNKPGSGISRKVEDEEDRQKIKNMMKQIKIPETM
                     GFIVRTAGYNRTKQEISRDYQHLVRLWKEINKKAKSIGKTSMIYQETDFGVRSIRDYL
                     TSEIEEIWVDDLETFRKMRAYLRAISPRYIKLLKHYKEKTPLFDRHNLEDQIRVIYQE
                     RVELKSGGHIVINPTEAMITIDVNSGRGSNKKNIEETAFKTNIEACEEIARQLRLRDL
                     GGLIAIDFIDMMDKKHIAEVEKSFKKALSIDKARIQLSRISKFGILELSRQKKQSTIQ
                     EISYMKCPYCKGSGLRPSLEYAALGAFRKVESEAVKGLCCELKINLPHEIADYLLNRK
                     RSELLDLESSFDVTLTICGNSEIPWDRVDIQQIAKEHIDSADHPQEADEPKGQEAPAT
                     AEEEKAVPGGEKPARKRGRRRPRHKKR"
     gene            complement(1715..2323)
                     /locus_tag="PLF77_10910"
     CDS             complement(1715..2323)
                     /locus_tag="PLF77_10910"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPC09408.1"
                     /translation="MLFSINSQNPQMRLIKKAADILRDGGIVIYPTDTVYGLGCDLSN
                     KKGIEKIYELKRRNRKQPLSFVCSDLKHISEYAKVTDYAYKTMKRLLPGAYTFILEAS
                     RLVPKIILPKRSTTGIRVPDNQICLALVRELGQPIISTSVKTEKGETLGDPAVIKEYF
                     GKTVDLIIDGGIIVPEPSSVISLVDDNIDIIRKGKGDISAFL"
     gene            complement(2489..>3081)
                     /locus_tag="PLF77_10915"
     CDS             complement(2489..>3081)
                     /locus_tag="PLF77_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011417634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HPC09409.1"
                     /translation="SDLAGKSNVEYKAKELGIDLEKNDAVAKKIVHEVKLMEDRGFQF
                     DAADGSLSLLIKKTIGEFVAPFNLETFSVITSRKENNPCLSQATIKISVAGEEELTAA
                     EGNGPVNALDHALRKALTKFYPQIKNMRLVDFKVRTLEGSEGTGAGVRVLVDSTDGED
                     VWSTIGVSENIIEASWQALVDSIQYKLSKENNLNRR"
BASE COUNT          611 a          822 c          764 g          884 t
ORIGIN      
        1 ttcctttttt tatgacgcgg tctgcgccgt cctctttttc tggcgggttt ttccccgccg
       61 ggcacagcct tttcctcctc ggcggttgcc ggagcctcct gtcccttggg ctcatccgcc
      121 tcttgcggat ggtcggcgct gtcaatgtgc tccttcgcga tctgctgaat gtccacccga
      181 tcccagggaa tttcagaatt cccgcaaatc gtcagcgtga catcaaatga cgattccaga
      241 tcaagcagtt cgcttctctt gcgattgagc aggtaatcgg cgatctcatg gggaaggttt
      301 attttcaatt cgcagcagag ccctttgaca gcttccgatt cgactttccg aaaggccccc
      361 agcgccgcgt attccagaga aggacgcaag ccgctccctt tgcaatacgg gcacttcatg
      421 tagctgattt cctgaatggt cgattgtttt ttctgccggg ataattcgag gattccgaac
      481 ttggaaatcc gggaaagctg aatgcgggcc ttatcgatgc tcagcgcctt cttgaaggat
      541 ttttccacct cggcgatatg ttttttatcc atcatatcga taaaatcgat ggcgatcagc
      601 ccgccgaggt cgcgtagcct gagctgccgg gcaatttctt cacaggcttc aatattcgtt
      661 ttaaaagcgg tttcttcgat atttttcttg ttggagccgc ggccggaatt gacatcaatc
      721 gtgatcatgg cctccgtcgg gttgatgacg atgtgtccgc cggatttcag ttccacgcgt
      781 tcctgataga tgacgcggat ttgatcttcc agattgtggc ggtcgaaaag cggcgtcttc
      841 tccttgtaat gcttgagcag tttgatgtaa cgcggcgaga tggccctcag gtaggcgcgc
      901 atcttacgga atgtctccag atcatccacc cagatttctt cgatttccga tgtgaggtaa
      961 tcgcggatac tgcgcacgcc gaaatccgtt tcctgataaa tcatggaggt cttgccgatg
     1021 cttttcgcct ttttgttgat ttccttccag agacgaacca gatgctgata gtcgcgggag
     1081 atctcctgct tggtccggtt gtagcccgcc gtccggacga taaatcccat cgtttcgggg
     1141 atttttatct gcttcatcat atttttgatt ttctggcggt cttcctcatc ttccactttg
     1201 cgggaaatgc cgctgccggg tttgttgggc agcaaaacca gatatcgtcc gggcagggaa
     1261 atgtaggacg tgagcagcgc ccccttgttg ccgatggctt cccggacggc ctgcacgatg
     1321 atttcctgtc cgacttttaa agccggtttg ccgttggatt gtcctttttc cggttcggtg
     1381 aaatagtcgt gactgacgtc cttgagggga agaaaaccgt ctttgctgcc gccgtaatcc
     1441 acaaaggcgg cctgcaggcc gcgttcaact ttcagaacct tgcctttgta aatgttgccg
     1501 gtgatcggtt ccttggtcgg catttcaata ttgaaatcga ccagaattcc cttttcgtta
     1561 accgcagcca tccgtctctg ttctttatga acagcgttaa cgagcataat atgtttcata
     1621 aatgtccttt ttgcggcggc ttttttgcgc gcacccgcgc tccggcagcg catttaaaca
     1681 tctgcaggtt aaaattccgt tccgcgtttt atatttagag aaaagcgctt atatcgcctt
     1741 ttccttttct tatgatgtcg atattgtcat ccaccaggct gatgacgctt gacggttccg
     1801 gcacaatgat accgccgtcg ataatcagat cgactgtttt accgaaatat tccttaatga
     1861 cagcagggtc gcccagagtt tctccctttt ccgttttcac gctggtgctg ataatgggct
     1921 gtccgagttc ccggaccagc gccagacaaa tctgattgtc gggtaccctt atacccgttg
     1981 ttgaccgttt aggcaaaata atttttggga ccagacgcga tgcctccaga atgaaggtat
     2041 aggcgcccgg caaaagccgc ttcatggttt tataggcgta atcggttact ttggcgtact
     2101 cgctgatgtg tttcagatcg gaacagacaa agcttaacgg ttgctttcgg tttctccgtt
     2161 tcagttcgta aattttttca atcccttttt tgttggacaa atcgcagccc agaccgtaaa
     2221 ccgtgtcggt aggataaatg acgatgccgc cgtcgcgcag aatatcggcg gccttcttga
     2281 tcaggcgcat ttgcggattc tggctgttga tggaaaaaag cattctctgt ccgtcccttt
     2341 tgaaccttga agaaactcat ataccaaaaa aggttcttgc gcaacggaag cttcacggga
     2401 cgtccgccat gcaggatttg tgatcatccc cggttgtctg ttccgcgcgg caccgcgccc
     2461 gacacgaaag cccaaagaag gttgagcatc atcttctgtt gagattgttc tccttgctca
     2521 gcttgtattg aatgctgtcc accagcgcct gccagctggc ctcgatgata ttttcggaga
     2581 cgccgatggt gctccagacg tcttcgccgt cggttgaatc cacgagcacc cggacacccg
     2641 cgccggtgcc ttccgaccct tccagcgtgc gcaccttgaa atccaccagg cgcatatttt
     2701 tgatttgcgg ataaaatttc gtcagcgctt tgcgcagggc gtgatccagc gcattgaccg
     2761 ggccgtttcc ttccgcggcc gtcaactcct cttcgccggc caccgagatc ttgatggtcg
     2821 cctgagacag acagggattg ttttccttac gcgacgtaat cacgctgaag gtttccagat
     2881 tgaacggcgc gacaaattcg ccgatggttt tcttgatcag cagagaaagc gaaccgtccg
     2941 cggcgtcaaa ctgaaatccg cggtcttcca tgagcttgac ttcatgcacg atctttttgg
     3001 caacggcgtc gtttttctcc agatcaatgc ccagttcttt cgctttatat tccacgttgc
     3061 tttttcccgc caggtcggac a
//
LOCUS       DAOOLB010000151         3078 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_38588_length_3078_cov_53.678134, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000151 DAOOLB010000000
VERSION     DAOOLB010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38588_length_3078_cov_53.678134"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1336)
                     /locus_tag="PLF77_10920"
     CDS             complement(89..1336)
                     /locus_tag="PLF77_10920"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HPC09410.1"
                     /translation="MQKRIVITGMGTLNAIAHNIPDFSPALKNGHCGIREIDIFDTSG
                     FSARTGGQIKNFAARNYIPQNFSMKRMSRADLLSFAATLEALHDAGLYPFPEELQKDT
                     GVSIGGGAGGLLEAESFYANFLKSNGRRARFSRLASLFCASSADRIASAFGLAGPKAT
                     FMTACSGGATAIGYARDLIAGGQASVVIAGGVEPMCRLTYAAFNALKSVAAATCRPFD
                     KNRDGLSLGEAAAIVILEELDSALARGARIHGEILGYGITCDSYHMTAPDEQASGAVR
                     SMEAALRDSGLSPDDIDYINAHGTATPVNDAMETKAIKNVFGKRAYTIPVSSTKSMHA
                     HTLGAAGALEAIVSILALRDGFVPPTINHQERDPQCDLDYITSGARSANLKTALSNSF
                     AFGGNNTTLIFGKYTERSARHER"
     gene            1604..2131
                     /locus_tag="PLF77_10925"
     CDS             1604..2131
                     /locus_tag="PLF77_10925"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HPC09411.1"
                     /translation="MAKKNPETKNEEKVFVKERIIAKRLKNSKKEAADQDVKAARKAE
                     RLDEENEVLITILSEIENLPKEDILYNYSEDISMTGTRIQGNCLLPVDTFLKIDLVLK
                     NLKQTVTVFGKVKWSKPAEDVKSYEAGVEFVDTPEESIKKLGDYILSINQYKNLNPVG
                     VPYWIFAKFNKPSSK"
     gene            2404..2679
                     /locus_tag="PLF77_10930"
     CDS             2404..2679
                     /locus_tag="PLF77_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09412.1"
                     /translation="MKKTLRTIAVAAAVVIFTATFGFAEYAATGAADFPYFQLGCLIV
                     GGLILMTLKRKYEKMYVAEMVGAFALYTLLMALFTNPVIDAVKTMVM"
BASE COUNT          844 a          693 c          752 g          789 t
ORIGIN      
        1 tcgaccgaat tcttctttgc cgatgcccaa aggtgtcacc atgccgatac cgcttacaac
       61 cacgcgccgg gcatcatctt gcggtcggtc agcgttcatg acgtgcgctc ctttccgtgt
      121 atttgccgaa gatcagcgtg gtgttgttgc ctccgaaagc gaaggaattg gaaagcgcgg
      181 tctttaaatt tgcggatctt gcgccgctgg taatgtaatc gaggtcacac tgcggatcgc
      241 gttcctgatg attgattgtc ggcggaacga agccatcacg cagcgcgagg atggaaacaa
      301 tggcttccag agcgcctgca gcgcccagag tgtgcgcgtg catggatttg gtggaactga
      361 ccggaatcgt ataagcccgc ttgccgaaaa catttttgat ggctttggtt tccatggcat
      421 cgttgaccgg tgtggctgtg ccgtgcgcgt tgatgtaatc gatatcatcg ggagacagtc
      481 cgctgtcccg aagcgccgcc tccatggacc gaacggcgcc ggaggcctgt tcgtcggggg
      541 cagtcatatg ataagaatcg caggtgatgc cgtagcccag aatctctccg tgaattcgag
      601 ctcctcttgc cagcgccgaa tccagctctt ccaggatcac gatcgccgcg gcctcaccca
      661 gagacaggcc gtcccggttt ttatcaaatg ggcggcaggt agcagcggca accgatttga
      721 gtgcgttgaa ggctgcatat gtcagccgac acatcggctc cacgccgccg gcgatgacaa
      781 cggacgcctg gccgccggca attaaatcac gcgcgtagcc aatggcggtg gcgcctccgg
      841 aacaggctgt cataaacgtt gctttcggtc ccgccaaacc gaaagccgaa gcaatacggt
      901 cggcggaaga agcgcagaat aaagaagcaa gccgggagaa ccgggcacgc cgtccgttac
      961 tcttcaaaaa attcgcgtaa aaggattctg cttcgagcag tccgcccgcg ccgccgccga
     1021 tcgatacgcc cgtgtccttc tgaagttcct ccgggaaagg atataatccg gcatcatgca
     1081 aagcttccag tgttgcggcg aatgaaagca gatctgcgcg cgacattctc ttcatggaga
     1141 aattctgggg aatgtagttg cgggcggcaa aatttttaat ctggccgcct gtccgggcag
     1201 aaaatcccga tgtatcgaaa atatcgattt ccctgatgcc gcaatgaccg tttttcagcg
     1261 ctggcgagaa atcggggata ttatgggcga tggcgttgag cgttcccatg cccgtgataa
     1321 caattcgttt ttgcaaattc aaccctcatc tgatttttac ggcgattcta aaaatcgatg
     1381 taaaacttaa attgacgtcg gtaaaaagtc aaataaatat tccccgataa ccatgatcct
     1441 gcctcttttt gtttcgcttc cgtcttgcgt ttccccaatc cggatacggt tgtccggcag
     1501 ggccttcggg aaacggtcca aaaaaccgct tgtttttttg gtatcctgtt gcgataatat
     1561 taggaagact atcatttgat attttaatta ttaggagatc gtgatggcca aaaaaaatcc
     1621 tgaaacaaaa aatgaagaaa aagtcttcgt caaagaaagg atcatcgcca aacgactgaa
     1681 aaacagtaaa aaggaggcgg ctgatcaaga cgttaaagcc gcaagaaaag cggaacgtct
     1741 cgatgaagaa aacgaggtgc tgattaccat tctttccgaa atagaaaatc ttcccaaaga
     1801 agatatcctc tataattaca gcgaagacat ttccatgacc ggaacccgga ttcagggaaa
     1861 ttgtcttttg cccgtcgata catttttgaa aatcgattta gtattgaaaa atttgaaaca
     1921 aacggtaacg gtattcggca aggtgaaatg gagcaagccc gcagaagacg tcaagtctta
     1981 cgaggcgggc gtggagtttg tggatacgcc cgaggagtcc atcaaaaaac tgggagatta
     2041 tatcttgtcg atcaatcagt ataaaaatct taatccggtc ggggtgccct actggatttt
     2101 tgcaaaattc aacaaacctt catcaaaata aatttttatc ggtccgtttc aattttcaaa
     2161 tccgctttac atcgcttttg ccaaaaagca tttcgttctt atctttttgt atgtttcatt
     2221 aaacgtcttg aacaatttat taaaaaaatt tcggataacg gtctcagaga aaaaatccgg
     2281 ccgatgtcaa aagacaaata ttttattatt tcaatgacat agaaaataac gatcctttca
     2341 cgcaaactgg cacgataatt tcaataaata taggcaacaa cgcaggaaag gaggtaacag
     2401 atcatgaaaa agacattaag aacaatagcg gtggcagcgg cggttgttat ttttactgca
     2461 acgttcggct ttgcggagta cgcggcaaca ggcgcggctg attttcccta tttccaactg
     2521 gggtgcctga ttgtcggcgg gttgatcttg atgactctga aaaggaaata tgaaaaaatg
     2581 tatgttgccg aaatggttgg tgcgtttgct ctttacacgc ttttgatggc attgttcacc
     2641 aatccggtta tcgatgcagt taaaactatg gttatgtagc acgtgttaaa aagatttgaa
     2701 ggaacgaaaa cagaatgtaa ataaccgcta tcaacaaaaa tcatgatctg tttttatagt
     2761 ggttgccccc ttgaatcgga agattcaagg gggtttttaa tgatgttgat tgaggattgt
     2821 cctttatcgg aaaaaaagtt atagggataa aaagttcgtc tgaaaaacga acaattattg
     2881 acagatctca tggatgaatc gggttgatca tgaattttct ggccctggat tttgaaaccg
     2941 ccaattatta tcaggacagc gcctgtgctc tcggcttggt gcgtgtcgaa gataacaaaa
     3001 tcatccaccg gacatccttt ctgatcaggc cgccataccg gtattttgtc ttcacacaca
     3061 ttcacggtat tacctggg
//
LOCUS       DAOOLB010000152         3058 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_38907_length_3058_cov_52.454878, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000152 DAOOLB010000000
VERSION     DAOOLB010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3058
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38907_length_3058_cov_52.454878"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="PLF77_10935"
     CDS             complement(<1..1029)
                     /locus_tag="PLF77_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HPC09413.1"
                     /translation="MDESNRKPLLALVDGSNYIYRAFYGLPLLTNSKGFPTNAIYGFT
                     TMLLKLKRELKPDYIVITFDLKGPTARHEEFEDYKATRKPMPDDLVPQVPLIKDVVRG
                     FSVCVLEKQGTEADDLIGTLTVRAGEKGWRTVIISGDKDLLQLVNENVVMIDTMKDKT
                     YDAAAVKEKFGVGPDKVVEMLGLMGDTSDNIPGVPGIGPKTAQRLIEEYGTVENVISN
                     TENLKNVKLRESFRRHAEQARLSRQLANIRKDIPIDFDFQDAVVREPDREALSKLFTE
                     FEFSSLLQEIRTDQIKPVLECKMVVNEKALAEFAACLKESHEFSMEFIYEKSQPAHLI
                     GMAVSTGRH"
     gene            complement(1048..1494)
                     /locus_tag="PLF77_10940"
     CDS             complement(1048..1494)
                     /locus_tag="PLF77_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family transcriptional regulator"
                     /protein_id="HPC09414.1"
                     /translation="MDAIDKKILNILQKEFPLEEQPFLIVGKRCGISEEETIRRVQNM
                     KNDGIIRRIGAVFDGAHLGRVSTLCAARVPEEKIDTFVKVVNAGKNVTHNYLRNHEYN
                     IWFTVSAANAEELNVFLNEIKGKTGITDLLDMRSVRTFKINASFDL"
     assembly_gap    1568..1667
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1670..2728)
                     /gene="ahbD"
                     /locus_tag="PLF77_10945"
     CDS             complement(1670..2728)
                     /gene="ahbD"
                     /locus_tag="PLF77_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme b synthase"
                     /protein_id="HPC09415.1"
                     /translation="MKSEIDNTPRMIAWEVTRSCNLNCVHCRAASSLGPYSGELSTEK
                     CLQLIDQIAAVSTPVIILTGGEPLLRPDIFEIAAYGTNKGLRMVMATNGMLVTPAVAQ
                     KMTESGIQRVSISIDGKDAHSHDAFRQQKGAFNGAMQGIEALKNAKMEFQINTTITTN
                     NLDQIKDVFKLAKDIGAAAHHIFLLVPTGRGKDLAAQAITAADYEETLMWFHQESLSC
                     GIQLKATCAPHYFRIMHQNKPKEEKPVKKSGGHFHESTRGCLGGITFCFISHTGQVQP
                     CGYLELDCGNVKKKSFASIWENSEVFRNLRDYSKYEGKCGRCEFIKVCGGCRARAYEA
                     TGNYLAEEPLCLYQPKLK"
     gene            complement(2780..>3058)
                     /locus_tag="PLF77_10950"
     CDS             complement(2780..>3058)
                     /locus_tag="PLF77_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HPC09416.1"
                     /translation="EISLDVNEGADIIMVKPALAYLDILCRAKQEFDLPLAAYNVSGE
                     YAMVKAAAQLGWLDEDKAMLESLTAIKRAGADIIITYFAPQVANLLNK"
BASE COUNT          681 a          774 c          671 g          832 t
ORIGIN      
        1 atgcctaccg gtactgacag ccataccgat cagatgtgcc ggctgacttt tctcatagat
       61 gaattccatc gaaaattcgt ggctttcttt cagacatgca gcaaattcag ccagcgcttt
      121 ctcattgaca accattttgc attccagaac cggtttgatc tgatccgttc tgatttcctg
      181 caaaagcgag gagaattcaa attcagtgaa cagttttgac agagcctccc tgtccggctc
      241 tctgacgacg gcatcctgaa aatcgaaatc gatcgggata tcttttctga tattggcaag
      301 ctgccggctg agcctggcct gttcggcgtg cctgcgaaaa ctttcgcgta gtttgacatt
      361 ttttaaattt tcagtgttgc tgatcacgtt ttcaaccgtt ccgtattctt cgatcagcct
      421 ctgcgccgtc ttgggaccga tgccgggtac accgggaata ttgtcggatg tgtcacccat
      481 caatcccagc atttcaacca ccttgtccgg accgacgccg aatttttctt ttaccgcagc
      541 cgcgtcatat gttttatctt tcattgtatc gatcatcacg acattctcat taaccagctg
      601 cagcaaatcc ttgtcgccgg aaatgatgac cgtacgccag cctttttcgc cggcccgtac
      661 cgtcagcgtc ccgataagat catcagcctc ggtcccctgt ttttccaaaa cacaaacaga
      721 aaatccccga acaacatcct taatcagcgg aacctgcggg accagatcat ccggcatggg
      781 cttgcgcgtc gccttgtaat cctcgaattc ctcgtggcgg gcggtgggac ctttgaggtc
      841 aaaggtgatc acaatatagt ccggcttcaa ttcgcgtttc agtttcaaca gcatagtggt
      901 aaagccgtaa atggcattgg taggaaatcc cttggagttt gttaaaagag gaaggccgta
      961 aaaggcccgg taaatataat tgctgccgtc aaccaacgct aaaagcggtt ttctattgct
     1021 ttcgtccata taaaatcact ttatttttta caaatcaaaa gatgcattaa ttttaaacgt
     1081 tcttaccgag cgcatatcca gaagatcggt gatgccggtt ttccccttga tttcgtttag
     1141 aaaaacattg agttcttcgg cgttcgcggc gctgacggta aaccagatat tgtattcatg
     1201 attgcgcaga taattatgcg ttacattctt gccggcattg acaaccttaa cgaaggtgtc
     1261 gattttttct tccggcacac gggcagcgca aagcgtgctg acacgaccca gatgtgctcc
     1321 gtcaaagacg gcaccgatac gacggatgat gccatcgttc ttcatattct ggacgcgtct
     1381 gatggtctct tcttcgctga tgccgcagcg ctttcccaca atgagaaagg gctgctcctc
     1441 cagcggaaat tccttctgta aaatgtttaa tattttctta tctattgcat ccataatcta
     1501 ttttaatttc ggtaatctat tttaatttcg gttggtataa gcacaacggc tcttccgcta
     1561 aatagttnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnatc tattttaatt
     1681 tcggttggta taagcacaac ggctcttccg ctaaatagtt gcctgtagct tcataagcac
     1741 gcgcgcggca accgccgcac accttgataa actcgcagcg gccgcacttg ccttcatatt
     1801 tactgtaatc ccgcaggtta cggaacactt ccgaattttc ccatatgctc gcgaagcttt
     1861 tcttcttcac attgccgcaa tccagttcca gatagccgca gggttgcacc tggccggtgt
     1921 gcgaaataaa gcaaaatgtg attccgccca aacagcctct ggtcgattca tgaaaatgac
     1981 cgcccgattt cttcaccggc ttttcttctt tgggtttgtt ctgatgcata atgcggaaat
     2041 aatgcggcgc gcaggtggct ttgagctgga ttccacaact caggctttcc tgatgaaacc
     2101 acatcagcgt ctcttcgtaa tcagcggcag taatggcctg cgcggcaaga tctttccccc
     2161 ggcctgtcgg caccagcaag aatatatgat gagcggccgc accgatatct tttgctaatt
     2221 taaaaacatc cttaatctga tccaggttat tggttgtgat ggtcgtgttg atttgaaatt
     2281 ccattttagc atttttgagt gcttcgatgc cttgcatcgc cccgttaaac gcacctttct
     2341 gctgacgaaa tgcatcgtgg ctgtgcgcgt ctttcccgtc aatgctgatg ctgactctct
     2401 gaatgccgct ctccgtcatt ttctgagcaa cagccggtgt gacaagcatg ccgtttgtcg
     2461 ccatcaccat gcgcagaccc ttgttggttc cgtaggcggc aatttcaaaa atgtccggac
     2521 gcaggagcgg ctcaccgccg gtcaggataa tcactggcgt tgatacggct gcgatttgat
     2581 cgatcaactg caggcacttt tcagtggaga gctcccccga ataagggccg aggctcgaag
     2641 ccgcgcggca atggacacag ttgagattgc agctgcgcgt cacttcccac gcgatcatac
     2701 gaggcgtgtt gtcaatttct gatttcatca atcaagtcca tttccgtctt gtctttcggc
     2761 ctgcgccgga atgataaaat tatttattca gcaggttcgc cacctgcggg gcgaagtagg
     2821 tgatgataat atcggcgccg gcgcgtttga tggccgttaa cgattccagc atcgctttgt
     2881 cttcatcgag ccagccgagt tgcgcggccg ctttaaccat cgcatattcg ccgctgacgt
     2941 tatacgctgc caggggcaaa tcaaattcct gcttcgcgcg gcagagaata tccagataag
     3001 ccagagcggg tttcaccatg atgatgtccg cgccctcatt cacatccagt gaaatttc
//
LOCUS       DAOOLB010000153         3052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_39002_length_3052_cov_30.452119, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000153 DAOOLB010000000
VERSION     DAOOLB010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39002_length_3052_cov_30.452119"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(32..442)
                     /locus_tag="PLF77_10955"
     CDS             complement(32..442)
                     /locus_tag="PLF77_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HPC09417.1"
                     /translation="MKKQKYILDSYALLAYFQAEPAGLKVRDILKDALSTHTTAYLSV
                     ISLGEIYYIIARKIGEEKADASLEDISCLPVSLIDATKERILAAAHVKAQHAVSYADA
                     FVVAAAVEHSATIVTGDPEFKQTESLAPVLWLRP"
     gene            complement(414..683)
                     /locus_tag="PLF77_10960"
     CDS             complement(414..683)
                     /locus_tag="PLF77_10960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HPC09418.1"
                     /translation="MDTLHSTIVTVSSKGWVVIPSLFRRQIGLKPGMKMMVTEADGKI
                     VLTPQAIDPVDALYGKLAAEESLTRALLTDRKEERTREETKIHSG"
     gene            complement(849..2084)
                     /locus_tag="PLF77_10965"
     CDS             complement(849..2084)
                     /locus_tag="PLF77_10965"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPC09419.1"
                     /translation="MIQRNITQNILDALADTPVVLINGARQTGKSTLVQAIAEQAHPA
                     RYITLDTASVLAAARNDAAGFLAGIDGAVVIDEVQKAPELFAAIKADVDRRRQPGKFL
                     LTGSTNVLLVPGLSESLAGRMELLSLWPFSQGELAGVKETFIDALFADTFHLKNLPKE
                     DPHDLREKIIKGGYPEAINRLKEDRRQAWFESYMTTILQRDIRDMANIEGLTNLPRLL
                     AVIAARSPALLNFAELSRTLAMPQSTLKRYMTLLSATFLIQLVPAWSGNLTKRLVKTP
                     KIVMGDTGLMSHLLGVNQKRLNTGEMIGPLLENFIIMEIKKQITWSAIKPQIFHFRTQ
                     TGQEVDIVLEDRAGNIVGIEVKASATIHANDFKGLRAFAETAGKRFRRGIVLYTGTES
                     IPFGEALTALPVQILWHNK"
     gene            complement(2490..>3052)
                     /locus_tag="PLF77_10970"
     CDS             complement(2490..>3052)
                     /locus_tag="PLF77_10970"
                     /inference="COORDINATES: protein motif:HMM:NF025953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HPC09420.1"
                     /translation="EPRTRFFAHETAIIDDNVEIGEKTKIWHFSHILSGSKIGENCNI
                     GQNVVIGPDAIIGNKCKIQNNVSVYKGVTLEDGVFCGPSMVFTNIFNPRAEIGKMDQV
                     RPTLIKKGATLGANCTIVCGHTIGRYAFIGAGAVVTKDVPDHALMVGNPARQIGWMCE
                     CGEKLNTKQKCPACKKQYKPMGDTLK"
BASE COUNT          705 a          688 c          808 g          851 t
ORIGIN      
        1 gggttagggg ttaggggtca taggtcatag gttatgggcg aagccagaga acgggggcta
       61 gtgattcagt ttgtttaaat tccggatcgc ctgtaacgat tgtagctgaa tgttccacag
      121 ccgcggcaac tacgaatgca tctgcgtagg aaacagcatg ttgcgcttta acatgcgcag
      181 cggcaagaat acgttctttg gtggcatcga tcaaactgac aggcaaacag gatatatctt
      241 ccaggcttgc gtcggctttt tcttctccta ttttacgggc tataatgtag taaatttcac
      301 cgagactgat tactgataga tatgctgtag tatgtgttga caatgcatcc ttcaggatat
      361 cccgcacttt caaacccgca ggttccgcct ggaaatacgc aagcagggca taactatcca
      421 gaatgtattt ttgtttcttc acgggttctt tcctctttcc ggtcggtcag caatgccctt
      481 gtcagggatt cctccgcagc tagtttgcca taaagcgcat caacagggtc aatggcttgc
      541 ggtgtgagca caattttgcc atctgcttca gtcaccatca ttttcatgcc gggcttcaaa
      601 ccgatttgcc gcctgaacag ggaaggaatg accacccatc ccttggaaga aaccgtgaca
      661 attgttgaat gtaaagtgtc cataatgacc tcctgacgtt atacgctttc tgaaacatag
      721 tataacgttt tggggagttc gtcaagggat taagtgttga ggggttagag gtaatgggta
      781 atgtattaag tattaagagg ttaggggtaa taggtaatgg gttaggggtt ataggacagt
      841 aagagattct atttgttgtg ccatagtatc tggacgggca gtgcggtaag ggcttctcca
      901 aagggaatgc tctccgttcc ggtatataaa acgatgcccc gccggaaacg cttgcccgcc
      961 gtttccgcaa atgcccggag tcccttgaaa tcattcgcat gtatcgtcgc gcttgccttg
     1021 acttcaatgc cgacaatgtt tccggctctg tcttcaagaa cgatatcaac ttcctggcct
     1081 gtctgggttc gaaaatgaaa aatttgtggt ttgatggcgc tccaggtgat ttgctttttt
     1141 atttccatga tgatgaagtt ttccagtaac ggaccgatca tttcgcctgt atttaatcgt
     1201 ttttgattta cacccagcag gtgtgacatg agtcctgtat cgcccatgac aatttttggc
     1261 gtttttacca gtcttttcgt caggtttcct gaccatgcag gcacaagctg gataaggaat
     1321 gtcgccgaaa gcagggtcat gtatcgcttg agcgtgctct gcggcatggc cagcgttcgg
     1381 gatagctccg caaaattcag aagcgccggt gaacgtgcgg caataacagc aagaagccgg
     1441 ggcagattgg tcaaaccttc gatgttggcc atatccctga tgtcccgctg cagtatggtt
     1501 gtcatatagg attcgaacca cgcctgccgc ctgtcctctt tcaatctgtt gatcgcttcc
     1561 gggtagcccc ctttgatgat tttttcgcga aggtcatgcg gatcttcctt cggcaaattt
     1621 ttaagatgaa atgtatcggc aaataacgca tcgataaagg tttccttaac gcctgcaagt
     1681 tcaccctgcg agaaaggcca aagggaaagc agctccatcc tgccggcaag ggattcggat
     1741 aaaccgggaa ccaaaaggac attggtggaa ccggtgagta gaaatttgcc cggttgtcgc
     1801 cggcgatcca cgtcggcctt aatggctgca aagagttccg gcgccttttg cacttcatca
     1861 atgacgactg ctccgtcgat tccggcaaga aaaccggcag catcatttcg ggcagcagcc
     1921 agtacggacg ctgtgtcgag tgtaatgtac cgtgcaggat gggcttgttc tgcaatggcc
     1981 tggacaagcg tacttttacc ggtctgacga gcgccattga taaggacaac cggcgtatcg
     2041 gccagggcgt cgagtatgtt ttgcgttatg tttcgttgga tcatggttga attataacca
     2101 tttggtggtt gattatcaac cattaaatca acctgaagca ggataattag agtcggactt
     2161 ttaaggagca gagaagcccg gcactattga ggcttgtggc gtgcttcgca aaactactct
     2221 tcttcgtcat acaggcctgc gccaggcttg tggcgtgctt cgcaaaactg cataaaatta
     2281 acaatattgt cattcccgcg actaccctga gacgtttgca taacaacaga aaaccaagaa
     2341 ttttgtcatt cacgcttgtg cccttcaggg tacgaaggcg ggaatccagt tttttcaatg
     2401 actttctgga taccggcact cgccggtgtg acgaaaaaag gagttgtgca acgctctcgg
     2461 tttcatcaag atttacttct ttaacttaat tacttaaggg tgtcccccat tggtttgtat
     2521 tgttttttgc atgctgggca tttttgtttt gtattcagtt tctcgccaca ttcgcacatc
     2581 cagccgattt gacgggcggg gttgccgacc atcagagcgt ggtcggggac gtctttggtc
     2641 acgacagcgc cggcgccgat gaaggcgtag cggccgatgg tgtggccgca gacgatggtg
     2701 cagttggcgc cgagtgttgc gccttttttg atgagggttg gacgcacctg gtccatcttg
     2761 ccgatttccg cacgagggtt gaagatgttg gtaaagacca tcgaggggcc gcagaaaacg
     2821 ccgtcttcca gtgtgacgcc tttataaacg gagacgttgt tttgaatttt gcatttgttg
     2881 ccgatgattg cgtcgggacc gatgacgacg ttctggccga tgttgcagtt ctcgccgatt
     2941 ttggagccgg aaaggatgtg ggagaagtgc cagatcttcg ttttttcgcc aatttcaacg
     3001 ttgtcgtcga tgatagctgt ttcgtgggcg aaaaaacgag ttctaggttc aa
//
LOCUS       DAOOLB010000154         3004 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_39890_length_3004_cov_38.893862, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000154 DAOOLB010000000
VERSION     DAOOLB010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39890_length_3004_cov_38.893862"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PLF77_10975"
     CDS             <1..292
                     /locus_tag="PLF77_10975"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPC09421.1"
                     /translation="VMVILAGFTQHVAQGTSLLVMIPSGAVGALTHWKMGNTEQGILW
                     GLIPGILLGTYIGGTVANIIPNDTLRLIFAGMLIFLGIRYFGMSSPRRIGKG"
     gene            295..1314
                     /locus_tag="PLF77_10980"
     CDS             295..1314
                     /locus_tag="PLF77_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09422.1"
                     /translation="MKKTDGYRKFSFFTDVRGMKNAIGEMRGWGHPHQPIFNAGYQRL
                     PDDPVFNEDWFISTIKEKIATHPENTMEYPFYGEKIFTEPLIGFIQGDDPIFQQYKTI
                     IGPHHFTPEEIMQWQARQHGVPAPRAEDLSVVSFILPLTKITKNDNAAMKEWSAERWA
                     QTRLLGEIFSQIFVREIVTCLMKRGILAIAPDVTPMFNKKRYPKVGWASPWSHRHMAY
                     AAGLGTFGMHDFFITEKGCAHRTGSFVVNVKLTSNRERPDDIHAHCLQYQGKSCMQCA
                     KRCPVNAITAENGHNKEVCYQRVAKSLIHCNKNYHIFIYGCGLCATGVPCESEIPKIL
                     QKNSK"
     gene            complement(1458..2570)
                     /locus_tag="PLF77_10985"
     CDS             complement(1458..2570)
                     /locus_tag="PLF77_10985"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPC09423.1"
                     /translation="MIKSRLCDMTGIKYPIIQAGMGPFSNNNLGVAAANAGVLGLFST
                     SGLCNKDFLPFIYNDWIKSAEANPDDDIPTAIEKLLKRAYRLTKDTNGVFGINVMVSA
                     ELLFQAELIINAAIQVRAANPGMEKHFKVIFSSAGDPVPWKEKIRGAGFTWMHVVPSV
                     KGALRCKKAGVDVIVASGHEGGFHTSWEPVHSMILLPAVVDAVSDEKTLVCGAGGFCD
                     GKTLAAALVMGADGVQMGTRFLATQESDFPPIWRQGVVEAGDRGTLVARGFVGPARWL
                     KNPRSEEHAQNTLQKSPGCYLGTPDDMLSIDFKLIEFEMESMQAAYDGNKEKALLAAG
                     EAAQRINDMPKVADMVQSMMKETEDILKGVQTKMLA"
     gene            complement(2610..>3004)
                     /locus_tag="PLF77_10990"
     CDS             complement(2610..>3004)
                     /locus_tag="PLF77_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPC09424.1"
                     /translation="EKEHTIENASVIICGGRGMGNKEKFKKLQELARLLGGDVGATRP
                     VVYAGWADHDALVGQAGKHIKPKLLFSFGISGAIQHTAGLGEAHFIIAINKNPQATML
                     KMADVAIIGDASDVLNHMIRELKKRIRE"
BASE COUNT          732 a          772 c          715 g          785 t
ORIGIN      
        1 cgtgatggtc attcttgccg gctttaccca gcatgtggca cagggaacat cgcttctggt
       61 catgattccc tcgggcgctg tcggcgcact cacacactgg aaaatgggca atactgaaca
      121 aggaatcctc tgggggctta ttcccgggat tcttttaggc acatacatcg gaggaactgt
      181 ggctaatatc atcccgaatg ataccctgcg cctgattttc gccggcatgc tgatttttct
      241 aggcattcgt tattttggga tgtcttcccc ccgaagaata ggcaagggtt gattatgaaa
      301 aaaacagatg gctatcgtaa attttcattt ttcaccgacg ttcgcggaat gaaaaatgcg
      361 atcggtgaaa tgcgtggatg gggccatcct catcagccaa tatttaatgc cggttatcag
      421 cgcttgcctg atgatcccgt attcaatgaa gattggttta tcagtaccat caaagaaaag
      481 atagccactc atccggaaaa taccatggaa tatccctttt acggagagaa aatcttcacg
      541 gagccgctga taggatttat tcagggtgac gatccgatat ttcaacagta taaaacgatt
      601 atcggaccgc atcacttcac gccggaagaa attatgcagt ggcaggccag gcaacatggc
      661 gttcccgctc cccgggcaga ggatttaagc gtcgtatctt tcatcctgcc gctgacgaaa
      721 attacaaaga acgataatgc ggccatgaaa gaatggtccg cggaacgttg ggcgcagacg
      781 cgcctcttag gtgaaatatt cagccagata ttcgtgcgtg aaatagttac ctgtcttatg
      841 aaaaggggca ttttggccat agcgccggac gtcacgccca tgtttaataa aaaacgttat
      901 cccaaagtag gctgggcttc tccctggtcg caccggcata tggcgtatgc tgccggtctg
      961 ggaaccttcg ggatgcacga ttttttcatc actgaaaaag gctgcgctca cagaacgggc
     1021 agttttgtcg tcaatgtaaa acttacatca aaccgtgagc gacctgatga tattcatgct
     1081 cattgtctgc aatatcaagg caaatcctgc atgcaatgcg cgaaacgctg tccggtgaat
     1141 gcgataaccg cggaaaatgg ccataataag gaggtttgct atcaaagggt ggccaaatct
     1201 ctgatacact gcaataaaaa ttatcacatt tttatctatg ggtgcggtct ttgcgcaacc
     1261 ggagtcccct gtgaatccga aatcccgaaa atcctgcaaa aaaattcaaa atgaaaataa
     1321 caaaaaaacc ggggtttatt ccggcttttt tgttatttgt ttttaaagtt tcagcttttt
     1381 aggagctgtt caaaaaagct tagacacaag gcacgtgccc ggcattcgcc gggtttccct
     1441 ttattttaaa atgggtgcta cgcgagcatc tttgtctgga cgcctttcaa aatatcttcc
     1501 gtctccttca tcatggattg caccatatcc gcgactttgg gcatgtcgtt gatgcgttgc
     1561 gctgcttcgc ctgctgccag aagcgccttc tctttgttgc cgtcgtaagc agcctgcatc
     1621 gactccattt caaactcaat taatttgaag tcgatcgaca gcatatcatc cggcgttccc
     1681 agataacatc cgggcgattt ctgcagtgtg ttttgggcgt gctcttcact gcgcggattt
     1741 ttaagccatc gcgccggccc gacaaagccg cgggcaacca gagtgcctct gtcacctgcc
     1801 tcgaccacac cttgtcgcca gatcgggggg aaatcgcttt cttgtgttgc aagaaaacgc
     1861 gtgcccatct ggacgccgtc ggcgcccatc accagggccg cagccagcgt cttgccgtcg
     1921 cagaaaccgc cggctccgca taccaaagtt ttctcatcgg aaaccgcgtc tacaaccgca
     1981 ggcagcagaa tcatggaatg caccggttcc caggaggtat gaaatcctcc ctcgtgtccg
     2041 gacgccacga tgacatcaac ccctgctttt ttgcagcgaa gagccccttt tactgaaggc
     2101 accacatgca tccaggtaaa accggcgcct ctgatttttt ctttccaggg caccggatcg
     2161 cccgcagacg agaaaataac cttgaaatgt ttttccatgc ccggattggc tgcccgcacc
     2221 tgaatggccg cattgatgat aagctccgcc tgaaaaagca attcggcgga gaccattacg
     2281 ttgataccga ataccccgtt tgtgtcttta gtcaaccggt aggctctttt gagcaatttc
     2341 tcaattgcgg tgggaatgtc gtcatccggg ttggcctcgg cgctcttaat ccagtcattg
     2401 taaataaagg gcagaaaatc cttattgcag agtccgctcg tggaaaacaa gcccagcact
     2461 ccggcgttgg cggcggctac gccgagattg ttgttactga acggtcccat gcccgcctga
     2521 atgatgggat atttgattcc ggtcatgtcg caaagtcttg atttgatcat ataagacatc
     2581 tcctataaaa ttattgtatc ttatacacat cactctctga tgcgcttttt gagttctcga
     2641 atcatatgat tgagcacgtc gcttgcatca ccgatgatgg cgacatcggc cattttcagc
     2701 atggttgcct gcggattttt attgatcgca atgatgaaat gtgcctcgcc caagcctgcg
     2761 gtgtgctgga tcgcaccgct gataccaaag gaaaacagga gtttcggctt gatatgcttt
     2821 ccggcctgac ccaccagtgc gtcgtggtcc gcccatcccg cgtaaaccac cggacgggtg
     2881 gcgcctacat cgccgcctaa aagtctggcc agttcctgaa gttttttaaa tttttcctta
     2941 ttgcccatgc cgcggccgcc gcaaataata accgacgcgt tttcgatggt atgctctttt
     3001 tcaa
//
LOCUS       DAOOLB010000155         2976 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_40420_length_2976_cov_36.597740, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000155 DAOOLB010000000
VERSION     DAOOLB010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40420_length_2976_cov_36.597740"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..502)
                     /locus_tag="PLF77_10995"
     CDS             complement(83..502)
                     /locus_tag="PLF77_10995"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPC09425.1"
                     /translation="MNQKDRETILKKAQNEPFAKKFQLTFVDLQKGYAKVAMVFTPDM
                     ENIFGMAHGGAIFSLMDAAFEIASNSHGTMAVALNMSINYLASPEKGATLTAEAREIN
                     RTKKTAAYDIRATDDSGKLLASCQALVYRLEKPLPFL"
     gene            complement(1107..2267)
                     /locus_tag="PLF77_11000"
     CDS             complement(1107..2267)
                     /locus_tag="PLF77_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HPC09426.1"
                     /translation="MYRIDLLNKISEKGLSLFSDQYEYRTDIPDPDGILVRSKEMKDM
                     TLPLSLKAIARAGAGVNNIPIDKCSEKGIVVFNTPGANANGVKELVILGMLMACRNVV
                     GGIAWAKGLVGEGDKVPDLIEKGKSKFAGTEIMGKKLGVVGLGAIGTMVANTAEGLGM
                     EVWGYDPFLSIEAAWRLSRNTKKAASLDKLLAECDFITLHVPQNKDTKGFMNTEKFAV
                     MKKGAVLLNFARGGLVDTAALKKAIADGIVGCYVTDFPDEEVIKTDKVIPIPHLGAST
                     EESEENCAVMAAMQLMDFLENGNIKNSVNFPECALDKSGKKRITIANQNTPGMIEKIT
                     HLLADNKLNIGDMINKSRGNLAYNIIDLEGDISEDLINKITSIEGIIAVRVI"
BASE COUNT          754 a          673 c          700 g          849 t
ORIGIN      
        1 cggttatcat tgatatttag tgatgcaaat aagtatgaca tttttaggta aaatcagcga
       61 ccacttcata aataattgcc gcttataaaa acggcagcgg cttttccagc cgataaacca
      121 gcgcctgaca ggacgccaga agctttcccg aatcgtcggt tgcgcgtata tcgtaggcgg
      181 cggttttttt cgtcctgtta atttctctgg cttcggcggt cagcgtagcg cctttttccg
      241 gcgatgccag ataattgatg ctcatgttaa gggcaactgc catggtgccg tgtgaattgg
      301 aggctatttc aaaagccgca tccataagcg aaaaaatagc gccgccgtga gccatcccga
      361 aaatattttc catgtccggt gtaaaaacca ttgccacttt tgcgtatcct ttttgcagat
      421 cgacgaacgt taactggaat tttttggcga aaggttcgtt ctgcgctttt tttaaaatag
      481 tctccctgtc tttttgattc atgagtatta ctcctgcatg tttttctgga caattgatac
      541 gttcttgtaa cgctataata aaaagggggg taatctgtcc agctgaaaac aaaaacgctc
      601 aattttgatc ttgcttttgt ggtacaggct gcaatattcc gactactacc tatagtagtg
      661 caaaaatacg attaccacca catattgatt tttttcttgc tttttcaaaa aaatattata
      721 aaataactga aagtttgaaa gaaaatcttt tggcagagaa attgaggttt cggtttggaa
      781 ggttctttga agcaaacggt ttttagatgg caaaatgcca aagtttgttt aaactggaga
      841 gggaccgctt ctgacgggga attttcgatg tatcgtcggg gagatagcaa aacagaacat
      901 gatcatatgg caaacgccga atgaagcggt tgcaaatggt taaccaaatt ttaaaccctt
      961 gcctgaaggg cggatcgtaa aagatccgcc ctttttcttt cgttaaatgt tcaggcaagt
     1021 taaaatccgc cattgctgta tcgaaaaaaa agtgtctgac gaaggccgcc agacactttt
     1081 tgatatgaca cgtgacagat gttttcttag atgactctaa cagcgatgat tccttcaatg
     1141 gacgtgattt tatttatcag atcttcgctg atatcacctt ccaggtcgat gatgttgtac
     1201 gccagattcc ctctgctctt gttgatcatg tcgccgatgt tgagcttgtt atcggccagc
     1261 agatgcgtga tcttctcaat catgcccggc gtattctgat tggcaatggt aattcttttc
     1321 ttgcccgatt tgtccagggc gcactcaggg aaatttaccg agtttttgat gttgccgttc
     1381 tccaggaaat ccatgagctg catggcagcc atcacggcgc agttttcttc cgattcttcg
     1441 gtggaggccc ccagatgcgg aatggggatg actttgtctg ttttgataac ttcttcatca
     1501 gggaaatcgg taacgtaaca gcccacaata ccatcggcaa tcgccttctt cagggccgct
     1561 gtgtccacga gcccgcctct ggcaaaattc aggaggacgg cgcctttttt catgacagcg
     1621 aacttttcgg tgttcataaa tcctttggta tctttgtttt gcggaacgtg cagcgtgatg
     1681 aaatcgcact cagccagaag cttgtcgaga ctggctgcct ttttggtgtt gcgggagagc
     1741 ctccaggccg cttcaatgga gaggaacggg tcatatcccc agacttccat gcccaagcct
     1801 tcggcggtat tggcgaccat cgtgccgata gcgcccagac cgaccacgcc cagtttcttg
     1861 cccataattt cagttccggc aaacttggat ttgcccttct cgataagatc aggcactttg
     1921 tcgccttcgc caaccagacc tttagcccag gcgattcccc cgacaacatt gcggcaggcc
     1981 atcagcatcc ccaggatgac cagttccttc acgccgttgg cgttggcccc cggtgtatta
     2041 aaaacgacaa tgcctttctc tgaacatttg tcgatgggaa tattgttgac gccggcgccg
     2101 gcgcgggcga ttgctttcaa tgataacggc agggtcatat ctttcatttc tttacttctc
     2161 accaggatgc cgtccggatc gggaatatca gtgcgatact cgtactgatc tgaaaaaagg
     2221 cttaacccct tttcagaaat cttgtttaac agatcgatgc gatacataaa tacctcctat
     2281 tcctttctaa tgagacccaa atttcagaaa cgatgtccac tgaaacttgt aggtcgaatt
     2341 tttccgaggc gaagcatggc gggacattga gccatcccga acagcgtaga tgatgaacct
     2401 caaaatttgc ttcggggttc atacccgtga ataaggagtt ctggcaggaa aactcctgag
     2461 ctttgtcgcc atagtacaat acagattgaa tttatctcat ggatacgaaa ccatctgttt
     2521 tgcaggacga gtatagaatg ggtgctttta cgtgtcaagg aaatcttaat gagatccatt
     2581 gagagcaaac gaagtgatgc tcgaaatgcg agggatactg tccaataacc ggagtttatt
     2641 ggacagtatc ccccggtgca tagcgccgtg gaaactgtgt ttacggtaag gaaatatgaa
     2701 ttttaacgag acccagattt cagaaacgaa gtgcaccgaa atttgatggt cgaattatcc
     2761 ccgaggcgaa gccgagcggg ataaatgtgg gtctaattcc ccgcccccta gagcgcagtt
     2821 cctttggata ccatgctgtt gtcggcgggg ttgtttatca tatcaaatga gtcttggtag
     2881 atgtaaaaaa aggttccctt agggggaacc cttatttttc ctggtgggct gtgcggggat
     2941 tgaacccgcg gccaatggat taaaagtcca ctgctc
//
LOCUS       DAOOLB010000156         2917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_41576_length_2917_cov_85.407058, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000156 DAOOLB010000000
VERSION     DAOOLB010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41576_length_2917_cov_85.407058"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1299)
                     /locus_tag="PLF77_11005"
     CDS             complement(70..1299)
                     /locus_tag="PLF77_11005"
                     /inference="COORDINATES: protein motif:HMM:NF021207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02710 family CRISPR-associated CARF protein"
                     /protein_id="HPC09427.1"
                     /translation="MKKVMILSLGGSPEPLKKSIAEHRPERLIFFASQESNAKLGEVL
                     QIEGYKPLKIETEIAENPNLLYECYKAARRCVDRASRLGVPDNDILVDYTGGTKVMSA
                     ALLLATAGYAFSFNYVGGDIRSKDGLGVVQNGYEKMYADMNPWAAFAEEERRQIVTLF
                     NARRFSSAIQILDLCKRELPAQINAFFQFVRPLANGFLFWEQFRHKEALDCLKKGTAL
                     VDEYLKHYADDHYRQLRENLQDHIDYLSDILKKTSGLQNFDSILVKELINNARRRMED
                     KRFDDAAARIYRALELYGQICFEKKIGCSNDKVKPAKIPQELRDEFIRKYRDPQSDMM
                     KLPLQATFTVLCAVGHEAGEKFFKYEDKIKNIQSARNKSILAHGAQPVSEHAAQSILD
                     TVTDFVQMKEFFDFPKL"
     gene            complement(1346..1906)
                     /locus_tag="PLF77_11010"
     CDS             complement(1346..1906)
                     /locus_tag="PLF77_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09428.1"
                     /translation="MTSVAILRQLPSLFRYADVEKFTGNANVFLTRAQSKSLVTRIAG
                     GFYLNALREPAPGIEEIACFLRTPSYISCEWALNRHGVLLQAPTVLTVLTLSTSVGPS
                     RNVVSGGVTIEFSRIAPKLFTGFEAKEGYNLATPEKALLDTIYLRKRVPFPDELDFDA
                     IDRKKLVRLAEPFPAVVRKRLEEVLR"
     gene            complement(1903..2760)
                     /locus_tag="PLF77_11015"
     CDS             complement(1903..2760)
                     /locus_tag="PLF77_11015"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPC09429.1"
                     /translation="MKNYRETHIRKAEIAQLILLHELYRQKDSRSLIFQGGTALRWCY
                     GGSRFSEDLDFVTSLAPKSVQTMLQTALRGARNLMIAHFGPGTLALRPKSARGEALKC
                     FVDFRPEAAREKISVKLKFEELLPDKAPDVRQLVLSSLGSVAHLAATGDFRVPRASTV
                     LVAETPEEILSDKVRALLERRYLKGRDFYDLWHLHTVLKIPADMNIIQRKWTFYQAAF
                     VARRDFRFFQKPSKEEKSQMREAIEQDLSRFLPPEAMAVFRAGQYSDLLEAARALFEE
                     LSAKGVRLP"
BASE COUNT          685 a          761 c          743 g          728 t
ORIGIN      
        1 ggaagcgaaa gggatggcgg catatttgat ttttacgata tgaagttgtc aaagagcctg
       61 cgctgtttcc tataatttag gaaagtcaaa aaattctttc atctgaacga aatccgttac
      121 tgtatccagt atgctttgag ccgcgtgttc gctgaccggt tgtgcgccat gagccagaat
      181 ggatttgttg cgcgcgcttt ggatattttt gattttgtct tcgtatttaa aaaatttttc
      241 tcccgcttcg tggccaacag cgcaaagaac ggtaaatgtc gcctgaagcg gcagtttcat
      301 catatcgctt tgcggatccc ggtatttccg tatgaattca tcgcgcaatt cctgtgggat
      361 ttttgccggt ttgactttgt cgttggaaca gccgattttc ttttcaaaac agatctgacc
      421 ataaagttcc aatgcccggt agatacgggc ggccgcatca tcaaaccgtt tatcttccat
      481 tctgcggcgg gcattgttga ttaattcttt taccaaaata gaatcgaaat tttgcaggcc
      541 gctggttttt ttcagaatat cggatagata atcaatgtga tcttgcaggt tttcccgcag
      601 ttgccgataa tggtcgtccg cataatgctt cagatattca tcaactagcg cagtaccttt
      661 cttgaggcag tccagggctt ccttatgcct gaactgttcc cagaacagga aaccgttggc
      721 cagcggtcta acaaactgga aaaaggcgtt gatctgcgcg ggcagctctc ttttgcataa
      781 atcaagaatc tggatggcgg aagaaaagcg ccttgcattg aaaagcgtga cgatttgccg
      841 tctttcttct tccgcaaaag ccgcccaggg gttcatgtct gcatacattt tttcatatcc
      901 gttttgcaca actcccagac catctttaga gcggatatcg ccgccgacgt aattaaagct
      961 gaatgcgtat ccggctgttg ccagcaaaag agccgccgac atgactttgg tgccgccggt
     1021 ataatcaaca aggatgtcgt tatccggaac accaagacgg gatgcccggt ccacgcaccg
     1081 cctggcggcc ttgtaacatt cgtagagcaa attcggattt tcagcgattt ccgtctcgat
     1141 ttttaagggt ttataacctt caatctgcag cacttcgccc aatttggcgt tggattcctg
     1201 ggaagcaaaa aaaataagcc gctccggccg atgttcagcg atgctttttt tcaagggctc
     1261 gggcgatccg cccaaagata aaatcatcac ttttttcata agattgtctc cgttaattga
     1321 caattttctc aataatccta taatgttacc gcaaaacctc ttccaaacgc tttctgacga
     1381 cggccgggaa cggttcggcc aaccgaacca gttttttgcg gtcgatagcg tcaaaatcca
     1441 gttcatcggg aaacggcacg cgttttcgta aataaatggt gtcgagcagc gccttttccg
     1501 gcgttgcaag attgtagcct tcttttgcct caaaacccgt aaaaagcttc ggggcaattc
     1561 gggaaaattc gatggtaacg ccgcccgaaa cgacgttgcg cgacggcccc accgacgtcg
     1621 aaagcgtcag cacggttaag accgtgggcg cctgcagcag aacaccgtgg cgattgagcg
     1681 cccattcgca ggagatgtaa gacggcgtcc gcaaaaagca ggcgatttcc tcgataccgg
     1741 gggccggttc acgaagcgca ttgagataaa accctccggc aatccgcgtg accaggcttt
     1801 tggactgcgc gcgcgtgaga aaaacattgg cgttgccggt gaatttctcc acgtccgcat
     1861 agcgaaacaa agacggcaat tgtcgcagga tcgcaacgct cgtcacggca gacgcacccc
     1921 ctttgcggag agttcttcaa aaagggcgcg tgccgcttcc agtaaatcgg aatactgtcc
     1981 ggcccggaaa accgccatcg cctccggcgg cagaaagcgc gacagatcct gttcgatggc
     2041 ctcgcgcatt tggctttttt cttctttcga cggcttttgg aaaaatcgaa agtcgcggcg
     2101 ggcgacaaaa gccgcctgat aaaatgtcca cttcctctgg atgatgttca tgtccgccgg
     2161 aatctttaaa acggtatgga gatgccacaa atcgtaaaaa tcccgtcctt tcagatagcg
     2221 ccgctccaga agcgcgcgga ccttgtcgga gagaatttct tcgggcgttt ccgccaccag
     2281 gacggtgctg gcccgcggca cgcgaaagtc tcctgtggcc gcaagatgcg cgacggaacc
     2341 cagggaagaa aggacaagct gccggacatc cggcgctttg tccggcaaaa gctcttcgaa
     2401 tttcagcttg acggaaatct tctcacgggc ggcttccggg cggaagtcca caaagcattt
     2461 cagcgcctcg ccgcgggcgc ttttggggcg aagcgcgagc gtccccggac cgaaatgggc
     2521 gatcatcaga ttccgcgcgc ccctgagcgc cgtctggagc atggtttgga cggacttggg
     2581 ggcaagggac gtcacgaaat ccagatcttc cgaaaagcgg ctgccgccgt agcaccagcg
     2641 cagcgccgtg ccgccctgaa aaatcagaga gcggctgtcc ttctgccggt aaagctcatg
     2701 cagcagaatc aactgcgcga tttccgcctt gcggatatgg gtttcacgat aattcttcat
     2761 atttgtttac gatataatac atatttgtat tagaatgtaa acagttgatt ggggcgcact
     2821 tctacccggc ttgctctacg ccgccggctc gaccttcacg atttcccagc tattccccgc
     2881 cttgcgtacg gtaacgcggg ctttgacggg ttttttc
//
LOCUS       DAOOLB010000157         2867 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_42572_length_2867_cov_35.510669, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000157 DAOOLB010000000
VERSION     DAOOLB010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42572_length_2867_cov_35.510669"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLF77_11020"
     CDS             <1..482
                     /locus_tag="PLF77_11020"
                     /inference="COORDINATES: protein motif:HMM:NF014819.2"
                     /note="FabB; beta-ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HPC09430.1"
                     /translation="RGATILGEVIGFACNNNGGDMILPNLTGITNTLKLGVETAKINP
                     EEVDLISAHATATKMGDIIEAQAINAVFGCNPHVIGLKSYTGHTMAACGAIETIMALY
                     MMQEGFIAPTLNLENIDERCAMLKHVQKLTDHPVKTAAIQNFAFGGVNTMLMIKKFS"
     gene            559..1281
                     /locus_tag="PLF77_11025"
     CDS             559..1281
                     /locus_tag="PLF77_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPC09431.1"
                     /translation="MFGYLFILLFLYLPVYVFAKNRATALQNLNHIHLKIFFTLTKRL
                     APKTKYNIPDNLREIHSSVVVCNHLSYLDPILLVSLFRQQRTIVKHTFFKVPIFGWFL
                     KNYGYISSGSDTMMGAAMINNLEDIKQHLSSGGVLFVFPEGTRSRDGSLSPFNKGVFT
                     IARYCNAPLKLILIRGTNKLFQPGHFLFHTQEHNEIELRLIGSLNPDYKSKDFSIAAL
                     ADEARKIFEEKIDHLKSDNKGD"
     gene            1285..1842
                     /locus_tag="PLF77_11030"
     CDS             1285..1842
                     /locus_tag="PLF77_11030"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPC09432.1"
                     /translation="MELQEAILKRRSVRKFTDDVVTDEELKQIFEAVRWSPSWANTQT
                     WEFIVVRDKALMEKVAGTYSEKNPATKCSLSASALIVACAKTAISGCYGGKQVTSISN
                     WEFFDLGIAVQTLCLKAHEMGLGTVVVGLMNHEACEKILAVPEGHKVYAVIPIGRPAT
                     EPKAGPPRKSLSEMVFLNNLNKPMF"
     gene            2171..2635
                     /locus_tag="PLF77_11035"
     CDS             2171..2635
                     /locus_tag="PLF77_11035"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HPC09433.1"
                     /translation="MLNEDYKDILHALSDENVRFLLVGAYALAAHGYPRATMDIDIWV
                     MPSPENADAVLRALQRFGASLQNLTKEDLQKDGTIFQIGVAPRRIDIITAASGLQFEP
                     AYQHSIQLNIEEMEVRIPSIDDLILNKKATGRTKDLADIEILESLKNSEKRL"
BASE COUNT          817 a          686 c          610 g          754 t
ORIGIN      
        1 agcgcggcgc gactattctg ggagaagtca tcggttttgc ctgcaacaac aacggaggcg
       61 acatgatttt gccgaatctc accggcatca ccaacacgct taagctgggt gtggaaaccg
      121 ccaaaatcaa tccggaggaa gtcgacttga tcagcgcgca cgccacggcc accaagatgg
      181 gcgacattat tgaagcgcag gccatcaacg ccgtcttcgg ttgtaatcct cacgtcatcg
      241 ggttgaaaag ctacaccggt cacaccatgg ccgcctgcgg cgccattgaa accatcatgg
      301 ccctctacat gatgcaggaa ggctttattg cgccgacgtt gaatctggaa aatatcgatg
      361 aacgctgtgc catgttaaaa catgtacaaa aattaaccga tcatccggta aaaacagcgg
      421 ccattcaaaa tttcgctttt ggcggcgtca atactatgct gatgattaaa aagttttcct
      481 gagttgaaga tgtttctgtc ccgtattttt cattttgcag ttaatttatc cgtaacactg
      541 attttgtggt gctatttcat gttcggatac ctttttattt tactgttttt atatctgccc
      601 gtttacgttt ttgcaaaaaa ccgcgctact gctcttcaga atttaaacca tattcattta
      661 aaaatattct tcacgttgac gaaacggctt gcgcctaaaa caaaatataa tattcccgat
      721 aatttgcggg aaattcattc atcggttgtt gtctgcaacc atttatccta tctcgatccc
      781 attctgctgg tgtcgctttt caggcaacag cgaaccatcg tcaaacatac ttttttcaaa
      841 gttcccatct tcggctggtt cttgaaaaac tacgggtata tttcctccgg atccgatacc
      901 atgatgggcg cggccatgat caacaacctg gaagatatca aacagcatct gtcgtcgggc
      961 ggcgttttat ttgtttttcc ggagggcacc cggagccgcg acggcagtct ttcgccgttc
     1021 aacaaaggcg tctttacgat tgcccgttat tgcaacgcgc cattgaaact cattttaatc
     1081 cggggaacca acaaactgtt tcaacccggt catttccttt tccacactca agaacataat
     1141 gaaattgaac tccggttaat cggttcgctc aatccggatt acaaaagcaa agatttttcc
     1201 atcgctgccc tggctgacga ggccagaaaa atattcgaag aaaaaataga ccatttaaaa
     1261 tcagataaca aaggggacta ggttatggaa cttcaggaag ccattttaaa acgcagaagc
     1321 gtcagaaaat ttaccgacga tgttgtcacc gatgaagaac tcaaacaaat ctttgaggcc
     1381 gtacgctggt cgccctcctg ggccaatact cagacttggg aattcatcgt cgtccgcgac
     1441 aaagcgctga tggaaaaagt cgccggcacc tattccgaaa aaaacccggc cacaaaatgt
     1501 tctctaagtg cgtccgctct aattgtcgcc tgcgcgaaaa cagccatttc cggttgctac
     1561 ggcgggaaac aggtcacttc aatttccaac tgggaatttt tcgatctggg catcgccgtt
     1621 cagacgcttt gcctgaaagc ccatgaaatg ggacttggaa ccgtggttgt gggtttaatg
     1681 aaccacgaag cctgtgaaaa aattctggcc gtgcccgaag gtcacaaagt ttatgccgtc
     1741 attccgatcg gccgaccggc caccgaaccc aaggcaggac cgccgcgcaa atccctttcc
     1801 gaaatggtat ttttgaataa cctcaataag ccgatgttct aaatcattcc aacccaccgg
     1861 cagggagcta tctccctgcc tttccaaaaa gcgtgtaact tcagcttccg gctgcgttat
     1921 tcataaatga agatataaaa aatagcaaag tgtccccaga ttttcgaaat tttcggaacc
     1981 ggccacaggg aatatccggt tgattttact tataaaaaat ggtaatttaa atttatgaat
     2041 aaaaagcaaa ccgccataca ccgaatgacg gatcatcagg acgacaatta tgtccccggc
     2101 acgccggaag agcggattct gcttgtttgg ccgttgacac gtgaaatatc ctccctgagt
     2161 ataaagcatc atgctgaacg aagactacaa agacatttta cacgccttat ccgacgagaa
     2221 cgttaggttc cttcttgtcg gagcgtatgc acttgcggct catggttatc cccgggccac
     2281 gatggatatc gatatctggg tcatgccatc tccggaaaac gctgatgcgg ttctgcgggc
     2341 tttgcaacgt tttggcgctt cattgcagaa tttaaccaaa gaagaccttc agaaggacgg
     2401 aacaattttt caaatcggag ttgccccaag acggattgac attatcaccg cggcctcggg
     2461 gctccagttc gaacctgctt atcaacactc aattcagctg aatatagagg aaatggaagt
     2521 ccgtattcct tcgatcgacg accttatcct caataaaaaa gcaacaggaa gaacaaaaga
     2581 tcttgccgat attgaaatct tggaatcttt aaaaaattcc gaaaaacgtc tttgaaacaa
     2641 atgcacaaat aattctgata ctggtttagt cgtccttcga ttgaagccac tatgaatctc
     2701 gataagccga tgtgttaata tttaatttaa agcgatcatc cgcaaaatac ccagcgatga
     2761 tgaaaatatt ttcaaaataa gaaggggaag ttgaatgtta tgaaagcctc ttttatccgc
     2821 aaagttctga tcatgatcgt cagtctgtat gtgacgctga gaattac
//
LOCUS       DAOOLB010000158         2672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_46978_length_2672_cov_57.635843, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000158 DAOOLB010000000
VERSION     DAOOLB010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46978_length_2672_cov_57.635843"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            552..1148
                     /locus_tag="PLF77_11040"
     CDS             552..1148
                     /locus_tag="PLF77_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09434.1"
                     /translation="MNELPKLSFDEAALLFHSSHPSEFYLGLVVLFHSAPNVPKVWDW
                     FIEFFKNKNISDIPPILVYYFAHIPWHGDIAYHGEGFNKEIKAHVKKRFDEFNKQDVE
                     KLLCFIDKERGIARGTLGQSAEAIISSLSKRDNFLLEIIQDSQLAMSVRESAALIYAY
                     HHQKEALPVLKALSDQGSWYAGELVSFMQRNGWIDPYA"
     gene            1472..1831
                     /locus_tag="PLF77_11045"
     CDS             1472..1831
                     /locus_tag="PLF77_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09435.1"
                     /translation="MPKRQILIRDVPESLYEWIEKERHAKRVSQKEFLLKVLENAAVH
                     SSGEAGTAGPSAFSKMTLVYWKSKKFWLGKLLEHPEIMTQGRTLEELEKNIREAFLLM
                     ALDEVPEQYQIREVAMA"
     gene            complement(2067..2306)
                     /locus_tag="PLF77_11050"
     CDS             complement(2067..2306)
                     /locus_tag="PLF77_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09436.1"
                     /translation="MIEAQARRIPLKVLIMDNGCAMATGGQPVPEGLLERVLGGWTSF
                     VRRIDKPADPRAVREVLSRAQASERLEIVHAVFRG"
     gene            complement(2452..>2672)
                     /locus_tag="PLF77_11055"
     CDS             complement(2452..>2672)
                     /locus_tag="PLF77_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09437.1"
                     /translation="GISIGAIGTAMSQQHLRNVLAFLDMPTMGQPEVFLQAKDGLFDE
                     AGNIGADSKQFLQDWMDKYVAWVKKHTA"
BASE COUNT          705 a          609 c          644 g          714 t
ORIGIN      
        1 tttgtaacaa cggaatcctc ttgatctttt atgagagaat aaattcaccc tggagcccgc
       61 tgggctgctc cggctcagct tttcgttcag tgatgaatgg aaacacaaaa catgatgcca
      121 aaatactctg aggcaatcgt cacttctaaa cttgggatca attttgtaaa aacggtggtt
      181 gagtcgtcgg gttgtatctt tcacagaatc gatcaagagg atgatctcgg tatagacgca
      241 atcattgaat tggtgaaaga cggtcttcct ctgagcaagc agatcgctat ccaaataaaa
      301 tctgggcaat cattttataa tggacaatca aatcaatgcc tgattcctgt tggcaatcat
      361 tgtgaatact ggagtaaata ccctttgtcc gtctatggta ttgtatatgt gccatcactg
      421 aatttggcta actgggttga cataaaatgg tatctgaaac actctggacc atgtgttact
      481 attaaattcg atcgaacaaa gcaaacattt tcgataacat cagtttcacg aaaatctttg
      541 ttcccgcgat catgaatgag ttgcccaagc tttctttcga tgaagcagct ttattattcc
      601 attccagtca tccctcggag ttctatcttg ggttagttgt tttgtttcat agcgcaccaa
      661 acgtccctaa agtgtgggat tggtttatag aattttttaa aaataaaaat atctctgata
      721 ttcctcccat tctcgtttac tatttcgcac atattccatg gcacggtgat attgcgtatc
      781 atggagaggg cttcaataaa gaaataaaag cccatgttaa aaaacgcttt gacgaattca
      841 acaaacagga tgttgaaaag ctcttgtgct ttatcgataa agaaaggggc attgcacgag
      901 ggacccttgg tcaatccgca gaagcaataa tatcgtctct ttcgaaacga gataactttt
      961 tactggaaat aatacaggat agccaattgg caatgtctgt aagagaaagt gctgctctaa
     1021 tatacgccta tcatcatcaa aaagaagctc tgcctgtttt aaaggcttta tctgatcagg
     1081 ggtcttggta tgctggagag ctcgtttcat tcatgcagag gaatggttgg attgatccgt
     1141 atgcataaaa caccgaacaa gagcaatgca cccgatcgct acgctccggc tgattttttc
     1201 gtggacacag tgacactgtt tccttctcaa cttcccgttg tttttgaacc acaggtaagt
     1261 cgttgtctga ttgtgaaagc cagtagtgga cgtgattttc catgacatgc cttgcgtgta
     1321 tttattgtgt atttttcata acataggctc ataatatgtc aaggcaaatg ttggctggta
     1381 aagtgctggc gcacccgatg ccgccgcacc cgattgacat cgtcagggat atttgatatc
     1441 aatgttatca atattaccgg aggtgagtga aatgccaaag agacagattc tcatccggga
     1501 cgtaccggaa tcgttgtacg agtggattga gaaggagcgg cacgcgaagc gagtgtcaca
     1561 gaaggagttt ttgctcaagg tgctcgaaaa cgctgcggtc cattcatcag gggaagcagg
     1621 cacggcggga ccatcggcct tcagcaaaat gacgctcgtt tactggaaga gcaagaagtt
     1681 ctggctcggc aaactgctgg agcacccgga aatcatgacg caggggcgaa cgctggagga
     1741 attggagaaa aacatccggg aagccttcct gctgatggcg ctggatgagg ttcccgagca
     1801 atatcagatc cgtgaagtgg cgatggcatg aaacggcaag tactgcttcg gcggcttaaa
     1861 aaagagggct gcgaattgtt tcgttccggg ggcaaccatg acatctatgt gaacacgaag
     1921 acgggcaaga agcaacctgt cccgcgacac agcgagatcg acgagcacct ggcgcggcac
     1981 atcttgcgat atctcggact ggagcaatga gaatcttagc tggttcaaac ctgcatcttg
     2041 aactggaatt caattagtct gcgcgtttac ccgcggaata cggcgtgtac gatttccagc
     2101 cgttccgagg cctgcgctcg gcttaaaact tcccgcacgg cgcgcggatc ggcgggcttg
     2161 tcgatgcggc gaacaaagga tgtccaaccg cccagcaccc gctccagcag gccttcggga
     2221 acaggctggc cgccggtggc catcgcgcag ccgttgtcca taatcagcac cttcagggga
     2281 atccggcgcg cctgcgcctc gatcaatccc atctgacaca gtgacgctgt ttcctactca
     2341 acttcccgtt gtgctttaga cttttttgca atacttttat gcaatctgac agaaaatatt
     2401 ctgcggctgt gcaacgttgc cggtagtttt atgctgctct gtttttattt atcaggccgt
     2461 gtgctttttc acccaggcaa catatttatc catccagtcc tgcaggaatt gtttgctgtc
     2521 ggcgccgata ttgccggcct catcaaacaa tccgtctttt gcttgaagaa atacttccgg
     2581 ttgccccatc gtcggcatat ccagaaaagc aagaacattg cgcaaatgct gctgggacat
     2641 ggccgtgccg atagcgccga tggaaatacc ga
//
LOCUS       DAOOLB010000159         2524 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Smithella sp. isolate AMR_MDS_2712
            NODE_50890_length_2524_cov_43.054273, whole genome shotgun
            sequence.
ACCESSION   DAOOLB010000159 DAOOLB010000000
VERSION     DAOOLB010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864787
            Sequence Read Archive: SRR5007116
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Smithella sp. (wastewater metagenome)
  ORGANISM  Smithella sp.
            Bacteria; Thermodesulfobacteriota; Syntrophia; Syntrophales;
            Smithellaceae; Smithella.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 10:21:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,214
            CDSs (total)                      :: 2,187
            Genes (coding)                    :: 2,179
            CDSs (with protein)               :: 2,179
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 23
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 8 of 8
            Pseudo Genes (internal stop)      :: 0 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Smithella sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50890_length_2524_cov_43.054273"
                     /isolate="AMR_MDS_2712"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053614"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="33.8 N 118.28 W"
                     /collection_date="2015-04-22"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            99..221
                     /locus_tag="PLF77_11060"
     CDS             99..221
                     /locus_tag="PLF77_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09438.1"
                     /translation="MIDVDVKELIFLVVFIGIIAVPISLSVLFRKKKENKKDPG"
     gene            290..775
                     /locus_tag="PLF77_11065"
     CDS             290..775
                     /locus_tag="PLF77_11065"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HPC09439.1"
                     /translation="MFNPNKYLDRAGKGFNIIACAAVIIMMLLSAGDVLLRLIGKPIP
                     GAYEMVGFLGTLVVSFALGYTTLEKGHIAVELLFKRLPQRLQLVLQAFTDLICFLFFS
                     LLAYQAFVYALDIRQSGEVSATLQMPLYPFIVGMAFGFTLLGIILAADFFKSLQRAFS
                     R"
     gene            1109..>2524
                     /locus_tag="PLF77_11070"
     CDS             1109..>2524
                     /locus_tag="PLF77_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPC09440.1"
                     /translation="MNIQKRIAAGIVFSLFILFAATGGFAAEEKVSMEQWLKIINPVS
                     PKETPPGQAGKKTDTDAGEPAKPRDTQGIAVTIYWHMADDADVYLNGKPLRKYEPSFK
                     TRPDEAPHPAFSAAATLFDGDVFTVGGRRGGSFGLMLLAVDSTGTIIFQTDSQSWKVY
                     DPGERIDWFNPQVALASPSGPVTVQSDPWYPQKELNAKFGNKALSIWSAPDKTFAYLY
                     GVVSGAGPKAPPLPEEKVADDAGIPDLQDVAPFALQEPLDIHTLSIAQYQGAVTAAME
                     AFRQTMGELSPEEEKRFSAKWGAYFDYSTPESVAYFNKLNPLLAEFLTLRAAIATAAV
                     EFDGAWEEAVIAAGYESENGIREGLAIAEMQKNQLLTMQARLAQVVKEITAVGNPPDV
                     QEAKKAARKKHQETMKTAKKAIKAEKKREEQKNAQAAKDGKTAAEAPTAASSPATPAA
                     GEKPPEPIVVDRATVDKKYPSS"
BASE COUNT          622 a          623 c          684 g          595 t
ORIGIN      
        1 cagcgtttct gaaggaattt gatggtcggg ccgccatctg acgaaaatgt cggatggcgg
       61 ttttttttat gccaatctga ctggaaagga tgatgctcat gatcgatgtg gatgtaaaag
      121 aattgatctt tctggtggtg ttcataggaa tcattgccgt tccgatcagt ttgtccgtgc
      181 tgttccggaa gaagaaggaa aacaagaagg atccgggctg ataacgctcc tgatttgttt
      241 aaggaacttc atgtttccgg atacgggaaa aaaagccggg ggacgtctaa tgttcaatcc
      301 aaataaatat cttgatcgcg cgggtaaagg atttaatatc attgcctgcg ctgcagtgat
      361 catcatgatg ctgctttccg caggcgatgt tttactgcga ctgattggca aacccattcc
      421 cggcgcatat gagatggttg gatttcttgg gactcttgtg gtgtcattcg ccctcggtta
      481 taccaccctg gaaaaggggc atatcgccgt ggaacttctt tttaagagat tgcctcaacg
      541 cctgcagctg gtcctgcagg cgtttacgga tcttatctgt tttttgtttt tctcgctgct
      601 ggcttatcag gctttcgtct atgcgctgga catccgccaa agcggtgaag tttctgcaac
      661 ccttcagatg ccgctttatc cattcatcgt cggcatggcc tttggtttta cactgctggg
      721 tatcattctt gccgcagact ttttcaaatc actgcagagg gcgttttcca gataatttgc
      781 gcggagcaca tggctggttc cggataccct gtgaacggga gaatttcttc gtgaccgggc
      841 atcttcctgg cgatgtgtgc ctgttatcat gaaccgactg ggcataaaag ggcaaaaccc
      901 ggcccggcaa aaccctcttg acacgtaaat tcccgtcttt tatactcaaa tccgagaaat
      961 aatatggttt ccgtctcttt tatatcgatg gcctgaagga aatgtatctg atttcctgcg
     1021 ggtgccgcgt caaacgattg gaaagtgatc atgcaagcca cagttcttct taaccgtgaa
     1081 acaaaagccg gacgacagga gtcgaaaaat gaatattcaa aaacgaattg ccgcgggcat
     1141 tgttttcagt ttgtttattc tttttgccgc gacgggcggt tttgcggctg aagaaaaagt
     1201 ttcgatggag cagtggctga agattatcaa ccccgtcagc ccgaaagaga ctccccccgg
     1261 gcaagccggc aaaaagacgg acacggatgc cggggagccc gcaaaacccc gggatacaca
     1321 gggtatcgcc gttaccatct actggcatat ggcggatgac gccgatgtat atctgaacgg
     1381 aaaacctctg agaaaatacg aaccttcttt taaaaccagg ccggatgaag ctccgcatcc
     1441 tgcattttcc gccgctgcaa cgctctttga cggagatgtt tttactgttg gtggccgccg
     1501 cggcggcagt ttcggcctga tgcttctggc tgtcgattcc actggtacaa tcatttttca
     1561 gaccgattcg caatcctgga aagtctatga cccgggagag cgaatagatt ggttcaaccc
     1621 ccaggtagcc ctggcttcgc catccggacc ggttacggtt cagtctgatc cctggtatcc
     1681 ccagaaagaa ctaaacgcca aattcggcaa taaagctttg tcgatctgga gcgctccgga
     1741 taaaaccttt gcctatcttt acggcgttgt gagcggtgcc gggccaaaag ctccgccgct
     1801 gccggaagaa aaggtcgcag acgacgcagg cattcctgac cttcaggatg tggcgccttt
     1861 tgcgcttcag gagccgttgg acatccacac gctcagcatt gcgcaatacc agggcgcggt
     1921 aaccgcggcc atggaagctt ttcggcagac gatgggagag ctgtcccccg aagaggaaaa
     1981 acgtttcagc gcaaaatggg gagcgtattt tgattattcc acgccggaaa gcgttgccta
     2041 cttcaacaag ctcaatcctt tgctggcgga gtttcttaca ttgcgggcgg ccattgctac
     2101 ggctgccgtg gaatttgacg gggcgtggga agaagcagtc attgccgccg gttacgaaag
     2161 cgaaaacggc atccgggaag gcctggccat tgcggaaatg caaaagaacc aattgctgac
     2221 catgcaggcg cggctggcgc aggtggtcaa agaaattacc gccgtcggaa atccgccgga
     2281 tgttcaggaa gccaaaaaag cggcccggaa gaagcatcag gaaacgatga agacggccaa
     2341 aaaggcgatc aaagcggaaa agaaaaggga ggaacagaaa aacgcgcagg cagccaaaga
     2401 cgggaaaaca gccgccgaag cgccgacggc agcctcttcg cccgcaacgc ccgcggcggg
     2461 agagaaaccg cccgaaccga tcgttgtcga tcgggcgacg gtggacaaaa aatatccttc
     2521 atcc
//
